LOCUS       DACDIG010000001       129427 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.001, whole genome shotgun sequence.
ACCESSION   DACDIG010000001 DACDIG010000000
VERSION     DACDIG010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 129427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 129427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..129427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.001"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            598..1287
                     /locus_tag="IV247_000001"
     CDS             598..1287
                     /locus_tag="IV247_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8776103.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1274..2482
                     /locus_tag="IV247_000002"
     CDS             1274..2482
                     /locus_tag="IV247_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8776104.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2776..4047
                     /gene="serS"
                     /locus_tag="IV247_000003"
     CDS             2776..4047
                     /gene="serS"
                     /locus_tag="IV247_000003"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAP8776105.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4596..6080)
                     /gene="guaB"
                     /locus_tag="IV247_000004"
     CDS             complement(4596..6080)
                     /gene="guaB"
                     /locus_tag="IV247_000004"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAP8776106.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6585..7280)
                     /locus_tag="IV247_000005"
     CDS             complement(6585..7280)
                     /locus_tag="IV247_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAP8776107.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7370..8470)
                     /gene="ychF"
                     /locus_tag="IV247_000006"
     CDS             complement(7370..8470)
                     /gene="ychF"
                     /locus_tag="IV247_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAP8776108.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8479..8670)
                     /locus_tag="IV247_000007"
     CDS             complement(8479..8670)
                     /locus_tag="IV247_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAP8776109.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8691..9581)
                     /locus_tag="IV247_000008"
     CDS             complement(8691..9581)
                     /locus_tag="IV247_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAP8776110.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9568..10335)
                     /locus_tag="IV247_000009"
     CDS             complement(9568..10335)
                     /locus_tag="IV247_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP8776111.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10347..11060)
                     /gene="rsmG"
                     /locus_tag="IV247_000010"
     CDS             complement(10347..11060)
                     /gene="rsmG"
                     /locus_tag="IV247_000010"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAP8776112.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11292..13193)
                     /gene="mnmG"
                     /locus_tag="IV247_000011"
     CDS             complement(11292..13193)
                     /gene="mnmG"
                     /locus_tag="IV247_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAP8776113.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13210..14607)
                     /gene="mnmE"
                     /locus_tag="IV247_000012"
     CDS             complement(13210..14607)
                     /gene="mnmE"
                     /locus_tag="IV247_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAP8776114.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14951..15289
                     /locus_tag="IV247_000013"
     CDS             14951..15289
                     /locus_tag="IV247_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8776115.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(15457..16215)
                     /locus_tag="IV247_000014"
     CDS             complement(15457..16215)
                     /locus_tag="IV247_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP8776116.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16233..17051)
                     /locus_tag="IV247_000015"
     CDS             complement(16233..17051)
                     /locus_tag="IV247_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP8776117.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17048..17404)
                     /gene="rnpA"
                     /locus_tag="IV247_000016"
     CDS             complement(17048..17404)
                     /gene="rnpA"
                     /locus_tag="IV247_000016"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAP8776118.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17522..17656)
                     /gene="rpmH"
                     /locus_tag="IV247_000017"
     CDS             complement(17522..17656)
                     /gene="rpmH"
                     /locus_tag="IV247_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAP8776119.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18179..19513
                     /gene="dnaA"
                     /locus_tag="IV247_000018"
     CDS             18179..19513
                     /gene="dnaA"
                     /locus_tag="IV247_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAP8776120.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19711..20841
                     /gene="dnaN"
                     /locus_tag="IV247_000019"
     CDS             19711..20841
                     /gene="dnaN"
                     /locus_tag="IV247_000019"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAP8776121.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21070..21312
                     /gene="yaaA"
                     /locus_tag="IV247_000020"
     CDS             21070..21312
                     /gene="yaaA"
                     /locus_tag="IV247_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAP8776122.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21299..22423
                     /gene="recF"
                     /locus_tag="IV247_000021"
     CDS             21299..22423
                     /gene="recF"
                     /locus_tag="IV247_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAP8776123.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22420..24366
                     /gene="gyrB"
                     /locus_tag="IV247_000022"
     CDS             22420..24366
                     /gene="gyrB"
                     /locus_tag="IV247_000022"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAP8776124.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24386..26857
                     /gene="gyrA"
                     /locus_tag="IV247_000023"
     CDS             24386..26857
                     /gene="gyrA"
                     /locus_tag="IV247_000023"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAP8776125.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27093..27392
                     /locus_tag="IV247_000024"
     CDS             27093..27392
                     /locus_tag="IV247_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAP8776126.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27442..27954
                     /gene="ssb"
                     /locus_tag="IV247_000025"
     CDS             27442..27954
                     /gene="ssb"
                     /locus_tag="IV247_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP8776127.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27980..28216
                     /gene="rpsR"
                     /locus_tag="IV247_000026"
     CDS             27980..28216
                     /gene="rpsR"
                     /locus_tag="IV247_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAP8776128.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28359..30332
                     /locus_tag="IV247_000027"
     CDS             28359..30332
                     /locus_tag="IV247_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAP8776129.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30338..30790
                     /gene="rplI"
                     /locus_tag="IV247_000028"
     CDS             30338..30790
                     /gene="rplI"
                     /locus_tag="IV247_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAP8776130.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31064..32431
                     /gene="dnaB"
                     /locus_tag="IV247_000029"
     CDS             31064..32431
                     /gene="dnaB"
                     /locus_tag="IV247_000029"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAP8776131.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33211..34503
                     /locus_tag="IV247_000030"
     CDS             33211..34503
                     /locus_tag="IV247_000030"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAP8776132.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            35290..36153
                     /locus_tag="IV247_000031"
     CDS             35290..36153
                     /locus_tag="IV247_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8776133.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(36282..37904)
                     /locus_tag="IV247_000032"
     CDS             complement(36282..37904)
                     /locus_tag="IV247_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8776134.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            38153..38674
                     /locus_tag="IV247_000033"
     CDS             38153..38674
                     /locus_tag="IV247_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8776135.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(38860..40590)
                     /gene="spxB"
                     /locus_tag="IV247_000034"
     CDS             complement(38860..40590)
                     /gene="spxB"
                     /locus_tag="IV247_000034"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAP8776136.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            40755..41573
                     /locus_tag="IV247_000035"
     CDS             40755..41573
                     /locus_tag="IV247_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAP8776137.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(41676..41873)
                     /locus_tag="IV247_000036"
     CDS             complement(41676..41873)
                     /locus_tag="IV247_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8776138.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            42042..42620
                     /locus_tag="IV247_000037"
     CDS             42042..42620
                     /locus_tag="IV247_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP8776139.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(43665..44168)
                     /locus_tag="IV247_000038"
     CDS             complement(43665..44168)
                     /locus_tag="IV247_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP8776140.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            44527..45552
                     /locus_tag="IV247_000039"
     CDS             44527..45552
                     /locus_tag="IV247_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8776141.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNLMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            45875..46753
                     /locus_tag="IV247_000040"
     CDS             45875..46753
                     /locus_tag="IV247_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8776142.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            47229..47345
                     /locus_tag="IV247_000041"
     CDS             47229..47345
                     /locus_tag="IV247_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP8776143.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            48273..48611
                     /locus_tag="IV247_000042"
     CDS             48273..48611
                     /locus_tag="IV247_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8776144.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            48705..48935
                     /locus_tag="IV247_000043"
     CDS             48705..48935
                     /locus_tag="IV247_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8776145.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            49335..49706
                     /locus_tag="IV247_000044"
     CDS             49335..49706
                     /locus_tag="IV247_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP8776146.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(49980..50672)
                     /locus_tag="IV247_000045"
     CDS             complement(49980..50672)
                     /locus_tag="IV247_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8776147.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(50675..51460)
                     /locus_tag="IV247_000046"
     CDS             complement(50675..51460)
                     /locus_tag="IV247_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8776148.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            51697..53097
                     /gene="lacG"
                     /locus_tag="IV247_000047"
     CDS             51697..53097
                     /gene="lacG"
                     /locus_tag="IV247_000047"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP8776149.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            53122..53439
                     /locus_tag="IV247_000048"
     CDS             53122..53439
                     /locus_tag="IV247_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP8776150.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            53452..55125
                     /locus_tag="IV247_000049"
     CDS             53452..55125
                     /locus_tag="IV247_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8776151.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            55139..56770
                     /locus_tag="IV247_000050"
     CDS             55139..56770
                     /locus_tag="IV247_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAP8776152.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(57261..58826)
                     /gene="guaA"
                     /locus_tag="IV247_000051"
     CDS             complement(57261..58826)
                     /gene="guaA"
                     /locus_tag="IV247_000051"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP8776153.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(58885..59808)
                     /locus_tag="IV247_000052"
     CDS             complement(58885..59808)
                     /locus_tag="IV247_000052"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAP8776154.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            60004..60609
                     /locus_tag="IV247_000053"
     CDS             60004..60609
                     /locus_tag="IV247_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8776155.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            60917..61933
                     /locus_tag="IV247_000054"
     CDS             60917..61933
                     /locus_tag="IV247_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8776156.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            62074..63375
                     /locus_tag="IV247_000055"
     CDS             62074..63375
                     /locus_tag="IV247_000055"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAP8776157.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            63421..64083
                     /gene="deoC"
                     /locus_tag="IV247_000056"
     CDS             63421..64083
                     /gene="deoC"
                     /locus_tag="IV247_000056"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAP8776158.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            64104..64502
                     /locus_tag="IV247_000057"
     CDS             64104..64502
                     /locus_tag="IV247_000057"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAP8776159.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            64568..65650
                     /locus_tag="IV247_000058"
     CDS             64568..65650
                     /locus_tag="IV247_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8776160.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            65862..67424
                     /locus_tag="IV247_000059"
     CDS             65862..67424
                     /locus_tag="IV247_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8776161.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            67417..68550
                     /locus_tag="IV247_000060"
     CDS             67417..68550
                     /locus_tag="IV247_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8776162.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            68550..69506
                     /locus_tag="IV247_000061"
     CDS             68550..69506
                     /locus_tag="IV247_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8776163.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            69605..70771
                     /gene="deoB"
                     /locus_tag="IV247_000062"
     CDS             69605..70771
                     /gene="deoB"
                     /locus_tag="IV247_000062"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAP8776164.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            70795..71613
                     /locus_tag="IV247_000063"
     CDS             70795..71613
                     /locus_tag="IV247_000063"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP8776165.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            71630..72337
                     /gene="deoD"
                     /locus_tag="IV247_000064"
     CDS             71630..72337
                     /gene="deoD"
                     /locus_tag="IV247_000064"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP8776166.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(72635..73321)
                     /gene="gpmA"
                     /locus_tag="IV247_000065"
     CDS             complement(72635..73321)
                     /gene="gpmA"
                     /locus_tag="IV247_000065"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP8776167.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(73493..74074)
                     /locus_tag="IV247_000066"
     CDS             complement(73493..74074)
                     /locus_tag="IV247_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8776168.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(74207..74890)
                     /gene="rpiA"
                     /locus_tag="IV247_000067"
     CDS             complement(74207..74890)
                     /gene="rpiA"
                     /locus_tag="IV247_000067"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAP8776169.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            75209..75622
                     /gene="rpsL"
                     /locus_tag="IV247_000068"
     CDS             75209..75622
                     /gene="rpsL"
                     /locus_tag="IV247_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAP8776170.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            75706..76176
                     /gene="rpsG"
                     /locus_tag="IV247_000069"
     CDS             75706..76176
                     /gene="rpsG"
                     /locus_tag="IV247_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAP8776171.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            76270..78357
                     /gene="fusA"
                     /locus_tag="IV247_000070"
     CDS             76270..78357
                     /gene="fusA"
                     /locus_tag="IV247_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP8776172.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            78529..79716
                     /gene="tuf"
                     /locus_tag="IV247_000071"
     CDS             78529..79716
                     /gene="tuf"
                     /locus_tag="IV247_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP8776173.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            80123..80431
                     /gene="rpsJ"
                     /locus_tag="IV247_000072"
     CDS             80123..80431
                     /gene="rpsJ"
                     /locus_tag="IV247_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAP8776174.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            80462..81091
                     /gene="rplC"
                     /locus_tag="IV247_000073"
     CDS             80462..81091
                     /gene="rplC"
                     /locus_tag="IV247_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAP8776175.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            81119..81742
                     /gene="rplD"
                     /locus_tag="IV247_000074"
     CDS             81119..81742
                     /gene="rplD"
                     /locus_tag="IV247_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAP8776176.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            81742..82032
                     /gene="rplW"
                     /locus_tag="IV247_000075"
     CDS             81742..82032
                     /gene="rplW"
                     /locus_tag="IV247_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAP8776177.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            82071..82904
                     /gene="rplB"
                     /locus_tag="IV247_000076"
     CDS             82071..82904
                     /gene="rplB"
                     /locus_tag="IV247_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAP8776178.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNRAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            82947..83225
                     /gene="rpsS"
                     /locus_tag="IV247_000077"
     CDS             82947..83225
                     /gene="rpsS"
                     /locus_tag="IV247_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAP8776179.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            83248..83595
                     /gene="rplV"
                     /locus_tag="IV247_000078"
     CDS             83248..83595
                     /gene="rplV"
                     /locus_tag="IV247_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAP8776180.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            83609..84265
                     /gene="rpsC"
                     /locus_tag="IV247_000079"
     CDS             83609..84265
                     /gene="rpsC"
                     /locus_tag="IV247_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAP8776181.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            84268..84702
                     /gene="rplP"
                     /locus_tag="IV247_000080"
     CDS             84268..84702
                     /gene="rplP"
                     /locus_tag="IV247_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAP8776182.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            84692..84880
                     /gene="rpmC"
                     /locus_tag="IV247_000081"
     CDS             84692..84880
                     /gene="rpmC"
                     /locus_tag="IV247_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAP8776183.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            84905..85171
                     /gene="rpsQ"
                     /locus_tag="IV247_000082"
     CDS             84905..85171
                     /gene="rpsQ"
                     /locus_tag="IV247_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAP8776184.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            85229..85597
                     /gene="rplN"
                     /locus_tag="IV247_000083"
     CDS             85229..85597
                     /gene="rplN"
                     /locus_tag="IV247_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAP8776185.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            85634..85942
                     /gene="rplX"
                     /locus_tag="IV247_000084"
     CDS             85634..85942
                     /gene="rplX"
                     /locus_tag="IV247_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAP8776186.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            85970..86509
                     /gene="rplE"
                     /locus_tag="IV247_000085"
     CDS             85970..86509
                     /gene="rplE"
                     /locus_tag="IV247_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAP8776187.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            86528..86713
                     /locus_tag="IV247_000086"
     CDS             86528..86713
                     /locus_tag="IV247_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAP8776188.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            86749..87147
                     /gene="rpsH"
                     /locus_tag="IV247_000087"
     CDS             86749..87147
                     /gene="rpsH"
                     /locus_tag="IV247_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAP8776189.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            87179..87715
                     /gene="rplF"
                     /locus_tag="IV247_000088"
     CDS             87179..87715
                     /gene="rplF"
                     /locus_tag="IV247_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAP8776190.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            87876..88232
                     /gene="rplR"
                     /locus_tag="IV247_000089"
     CDS             87876..88232
                     /gene="rplR"
                     /locus_tag="IV247_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAP8776191.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            88253..88753
                     /gene="rpsE"
                     /locus_tag="IV247_000090"
     CDS             88253..88753
                     /gene="rpsE"
                     /locus_tag="IV247_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAP8776192.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            88768..88947
                     /gene="rpmD"
                     /locus_tag="IV247_000091"
     CDS             88768..88947
                     /gene="rpmD"
                     /locus_tag="IV247_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAP8776193.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            88991..89431
                     /gene="rplO"
                     /locus_tag="IV247_000092"
     CDS             88991..89431
                     /gene="rplO"
                     /locus_tag="IV247_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAP8776194.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            89431..90723
                     /gene="secY"
                     /locus_tag="IV247_000093"
     CDS             89431..90723
                     /gene="secY"
                     /locus_tag="IV247_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAP8776195.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGTM
                     FVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFVA
                     ILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASSF
                     IATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNPE
                     KLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDLP
                     QSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            90785..91432
                     /locus_tag="IV247_000094"
     CDS             90785..91432
                     /locus_tag="IV247_000094"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAP8776196.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            91623..91841
                     /gene="infA"
                     /locus_tag="IV247_000095"
     CDS             91623..91841
                     /gene="infA"
                     /locus_tag="IV247_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAP8776197.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            91874..91990
                     /gene="rpmJ"
                     /locus_tag="IV247_000096"
     CDS             91874..91990
                     /gene="rpmJ"
                     /locus_tag="IV247_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAP8776198.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            92008..92373
                     /gene="rpsM"
                     /locus_tag="IV247_000097"
     CDS             92008..92373
                     /gene="rpsM"
                     /locus_tag="IV247_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAP8776199.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            92401..92790
                     /gene="rpsK"
                     /locus_tag="IV247_000098"
     CDS             92401..92790
                     /gene="rpsK"
                     /locus_tag="IV247_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAP8776200.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            92870..93808
                     /locus_tag="IV247_000099"
     CDS             92870..93808
                     /locus_tag="IV247_000099"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAP8776201.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            93836..94216
                     /gene="rplQ"
                     /locus_tag="IV247_000100"
     CDS             93836..94216
                     /gene="rplQ"
                     /locus_tag="IV247_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAP8776202.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            94488..95327
                     /locus_tag="IV247_000101"
     CDS             94488..95327
                     /locus_tag="IV247_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8776203.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            95303..96172
                     /locus_tag="IV247_000102"
     CDS             95303..96172
                     /locus_tag="IV247_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8776204.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            96162..96959
                     /locus_tag="IV247_000103"
     CDS             96162..96959
                     /locus_tag="IV247_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP8776205.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            97162..97908
                     /gene="truA"
                     /locus_tag="IV247_000104"
     CDS             97162..97908
                     /gene="truA"
                     /locus_tag="IV247_000104"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP8776206.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(98363..101032)
                     /locus_tag="IV247_000105"
     CDS             complement(98363..101032)
                     /locus_tag="IV247_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP8776207.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFFYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(101270..101458)
                     /locus_tag="IV247_000106"
     CDS             complement(101270..101458)
                     /locus_tag="IV247_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAP8776208.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            101728..102090
                     /locus_tag="IV247_000107"
     CDS             101728..102090
                     /locus_tag="IV247_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP8776209.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            102142..103824
                     /locus_tag="IV247_000108"
     CDS             102142..103824
                     /locus_tag="IV247_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAP8776210.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            103941..105977
                     /gene="recG"
                     /locus_tag="IV247_000109"
     CDS             103941..105977
                     /gene="recG"
                     /locus_tag="IV247_000109"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAP8776211.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            106026..107027
                     /gene="plsX"
                     /locus_tag="IV247_000110"
     CDS             106026..107027
                     /gene="plsX"
                     /locus_tag="IV247_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAP8776212.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            107149..107391
                     /gene="acpP"
                     /locus_tag="IV247_000111"
     CDS             107149..107391
                     /gene="acpP"
                     /locus_tag="IV247_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP8776213.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            107659..108663
                     /locus_tag="IV247_000112"
     CDS             107659..108663
                     /locus_tag="IV247_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8776214.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            108664..109608
                     /locus_tag="IV247_000113"
     CDS             108664..109608
                     /locus_tag="IV247_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8776215.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            109608..110570
                     /locus_tag="IV247_000114"
     CDS             109608..110570
                     /locus_tag="IV247_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8776216.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            110597..111511
                     /locus_tag="IV247_000115"
     CDS             110597..111511
                     /locus_tag="IV247_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8776217.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            111537..113318
                     /locus_tag="IV247_000116"
     CDS             111537..113318
                     /locus_tag="IV247_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8776218.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            113666..114352
                     /locus_tag="IV247_000117"
     CDS             113666..114352
                     /locus_tag="IV247_000117"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP8776219.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            114372..117953
                     /gene="smc"
                     /locus_tag="IV247_000118"
     CDS             114372..117953
                     /gene="smc"
                     /locus_tag="IV247_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAP8776220.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            117962..118771
                     /locus_tag="IV247_000119"
     CDS             117962..118771
                     /locus_tag="IV247_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8776221.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            118782..119780
                     /gene="ftsY"
                     /locus_tag="IV247_000120"
     CDS             118782..119780
                     /gene="ftsY"
                     /locus_tag="IV247_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAP8776222.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            119967..122234
                     /gene="gshAB"
                     /locus_tag="IV247_000121"
     CDS             119967..122234
                     /gene="gshAB"
                     /locus_tag="IV247_000121"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAP8776223.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(122323..122775)
                     /locus_tag="IV247_000122"
     CDS             complement(122323..122775)
                     /locus_tag="IV247_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAP8776224.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            122925..123875
                     /locus_tag="IV247_000123"
     CDS             122925..123875
                     /locus_tag="IV247_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP8776225.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            123868..124827
                     /locus_tag="IV247_000124"
     CDS             123868..124827
                     /locus_tag="IV247_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP8776226.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            124824..125579
                     /locus_tag="IV247_000125"
     CDS             124824..125579
                     /locus_tag="IV247_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8776227.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHIGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            125602..126555
                     /locus_tag="IV247_000126"
     CDS             125602..126555
                     /locus_tag="IV247_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8776228.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(127199..127495)
                     /locus_tag="IV247_000127"
     CDS             complement(127199..127495)
                     /locus_tag="IV247_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAP8776229.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            127850..128206
                     /locus_tag="IV247_000128"
     CDS             127850..128206
                     /locus_tag="IV247_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP8776230.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            128216..129115
                     /locus_tag="IV247_000129"
     CDS             128216..129115
                     /locus_tag="IV247_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAP8776231.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT        41029 a        23658 c        27034 g        37706 t
ORIGIN      
        1 ttcatcagga gtgtttcccg attcgttttt ggctgccgta gcaactttta tatcatatca
       61 gagagacaag taattgtcaa ctctttttta aaagtttttt cgatttcgtt tttcgaaatc
      121 ttccagcaac tcatttatca taacttatcg tttgttattt gtcaagaact tttttgaagt
      181 ttttttcgat agaatcaagc ttcgcaagtg gttctttttt aacgactcag ttatcttatc
      241 atgtcatttc caacatgtca acaacttatt ttttattatt taataagtta ttggtgttct
      301 tttgaacaaa taataatata tcaacatcac atgtgaaagt caataagaaa atgcttattt
      361 atcaacattt ctagcttaca tgatcattgg ttagttctgg tcctttttcg ttcgtcttta
      421 taactccgag agacgtaaaa tccttttgtt ttcagtttaa tgttcatgtt cttgtcttat
      481 tcacaccaac tagatgtctc accttctaaa attttcctta acaaagtgct gcttttatct
      541 atttctgctt ttttttgcta gaataatagt aacaatatga ctctggagga tttatttatg
      601 aaaattttga ttgctgacga tgataaagaa atcgttgaat tattaagtat ctatgttcac
      661 aatgaggggt atgaagcagt aaaggcttat gatgggaaag aagcattatc taaaatcaga
      721 acaatccctg atattgaatt acttatttta gatatcatga tgcctgagat ggatgggatg
      781 caagtagtga aagagctacg aaaagaatcc caaataccaa tcattatgct gacagcaaaa
      841 actactgata tggataagat caaaggactg gtcgctggtg cagatgatta tgtgaccaag
      901 ccatttaatc cgttagaagt aatggctaga gtaaagtctt tattacggcg tacaaaaatg
      961 cagattgccc cagatcagcc tgatgtatta gaagtaaatg cattaatcat caataaagac
     1021 tctcatgaag ttaaaacaat cgatgggaaa gaaatccagt taacagcttt ggaattcggt
     1081 atcctctact tgcttgctag tcatcctaat cgagtattta gcgcggatga aatttttgag
     1141 cgtgtttgga aacaagaaag tattgtctct gctaaaactg tcatggttca cgttagtcat
     1201 ttaagagata agattgaaga agcaactggt ggtgaaaaag tcattcaaac cgtttgggga
     1261 gtgggctata aaattgacgc aagatagtaa acgagcaact gaaaaacgcc gtcggatcaa
     1321 tttgacttca aaagaaatca gcgaattgtt agcggaagga atcatcacga ttatcctttt
     1381 gcttctatta aatgtttcta ttctggttgt tatcagttcg gtgatcaaca gttcaccttc
     1441 tttgacgaat gcgatttggg attcaaagaa tatttttgca gaacgattga acactgattt
     1501 gttctggaac ggccggaatt ttattattcc ctttttcttt ctattagata ttggtgtttt
     1561 gtattggcgc ttgattcgaa gatatcgcca aatgcaattg cggcatatca tcagcgaact
     1621 tcactacatc gcaaacggaa attatgatca tcgtatccct tttgaactga gtggtgactt
     1681 aagccgagtg gtaacaagta tcaatggatt ggtggatagt accgtagcag cgattgaaga
     1741 cgaacgtaaa atcgaaaaat caaaagatga attgatcacg aatgtcagtc atgatatccg
     1801 taccccactc acgtccatca ttggatacct aggactgata gaagatggcc agtatcactc
     1861 agaagaagat cttttaaagt atacgcatac tgcttatatc aaagcaaagc agatgaaatc
     1921 tttagtggac gacttatttg aatacactaa ggtacgccag cctgctgtcc cggtgaattt
     1981 ttctgccttt gacatgatcc agttgatcga acagttagca gctgattttg agttggaagc
     2041 ttctaaaaaa aatatccaaa tacttgttca atcaaaagta gactccctta tcatggatgg
     2101 cgataccgaa aaacttgtac gtgtcttcaa taatcttttg acgaatgcgt tgaaatacgg
     2161 aaaaggagca acgaagatcg taatcgaagt agaacgtatc ggttcagaag ttgttgcaac
     2221 agtcaaaaat aacggagcaa tgatcccaca acaggcaatc gataatctat tcgaccgttt
     2281 ttatcgtgta gaagaatctc gttcacaagc aactggagga actggacttg gtttagcaat
     2341 cgctcaaagc attgtggctt tgcacggcgg atacatttat gcaaaatcag ataagcaatg
     2401 gacttctttc atcatgcacc ttccaatcaa gaaaaatgaa aaattaccta ttgatacaca
     2461 ggaagttatt gacgaaagct gaaatattcg ctattcttat tacaattatt agaataaatg
     2521 aaaaacacag aacccagtac actttattct ttttcagaga gctgatggca ggtgtaaatc
     2581 agcaaaggat aatttggaag ttccagttcg tttacgtgcc ctttcgaaat aagaaaggtc
     2641 ggttcaacag ccgttatgtg ttcaacaagt gcagctgacg ctgaacttgg gtggtaccgc
     2701 gaatttcaga ctgattcgtc ccaagaataa tgggatggat cagtcttttt ttattttcaa
     2761 aaaggaggaa atcatatgtt agatgtaaaa atgatgcgac aaaatattga agaagtacaa
     2821 gaaaaattga tgactcgggg cgtaaaaaaa gaaacattag ctgaattcat ggaactagat
     2881 gaaaatcgtc gtaagctact agtcaaaagt gaagaattaa aaaaatatcg aaatgatgtt
     2941 tctgctgaga tcgcccaatt gaagcgtgca aaagaagatg cttctgctaa aatcgctgaa
     3001 atgaaagaag tcggtggcaa catcaaagca ttggatactg aaattgctga agtggatgaa
     3061 aaattgaaac aaattgcaac tactttgcca aaccttcccc atgattctgt gccagttgga
     3121 acagatgaag aggacaatgt agaaatccgt cgttggagcg aaccacgtac atttgctttt
     3181 gaaccaaaac cacactggga aattgcagaa aatcttgata ttttagattt tgaacgtggc
     3241 gcaaaagtag ccggtagccg ttttgtctat tacaaaggat taggtgctcg attagaacgt
     3301 gctgtctaca acttcatgct tgatcagcat atttacgaac acggctatac tgaaatgatc
     3361 actccttata tcgtcaacag taaagcgatg tttggtacgg gacaatttcc aaaattcaaa
     3421 gaagatgttt tccaattgga gaatactgat ctaacattaa tcccaacagc tgaagtacca
     3481 ttaactaact attataatga tgaaatttta gatggtgcag atttacctat ttatttcact
     3541 gcactaagcc ctgcttttcg ttctgaagcc ggaagtgctg gtcgggatac acgaggactg
     3601 attcgtcttc accaattcaa taaagtagaa atggtcaaat tttcggatgt agagcattct
     3661 tacgaagaat tggaaaaaat gacagctgat gcagaagata tcttgaaaaa attgcatttg
     3721 ccttatcgtg tgatggcctt atcaactgga gacatgggct tttctgcagc taaaacttat
     3781 gacttagaag tatggatacc tgcacaagat acttatcgtg aaatcagttc ttgttctaac
     3841 tgtgaagatt tccaagcacg ccgcgcaatg atccgttatc gcgataatga tggaaaaatc
     3901 cactatactc atactctgaa tggttcagga cttgcagttg gccgtacagt agctgccatt
     3961 ttggaaaact accaaaatga agatggttct gtcacagttc cagaagcgct cgttccttat
     4021 atggggaatc tgaaagttat taaataaaga ggctgtgaaa aagccgctta gtttcgagta
     4081 ttaagaaaaa ttttgtgaaa atggctttcc acatttttgc ataaattgga cttaataccg
     4141 agaaactggc ttttgaacac catttatcga gaggttgtga aatcagcgaa ttaacctgag
     4201 tattagagaa aaaaatggaa aaatagtccc tcatatttta ccgtttgttg aactaatacc
     4261 gagggtcagc tgattgaaca ccgtttagtg gagaggtcgt aagcctaccc cccagctcct
     4321 agttacaagg acaaattcaa aaaacagctc ttcatatttt atgaatgttg tacttgtgat
     4381 tcggaggtgg caagggaaca ctgtttattc aaaaagaggc tgggacagaa gcgtttaact
     4441 ccaagaaata agaagaaatt cacgaaaatt gcttttcaaa tttttgtgaa tttcttctta
     4501 tttccgaagg agttgcttct acttccgccg tttatacgtg tttagagcgt gagacaaaag
     4561 tgttctttac ttttgtccca cgctctttaa tgtttttaag attcaactga atagtttggt
     4621 gcttcttttg tgatttgtac atcatgaggg tgtgattctt ttaatccact gccgctcatt
     4681 tggataaatt gcgcttcatc acgtaattgc tgtagattgg ctgccccaac ataacccatt
     4741 cctgaacgca aaccgccgat catttggaaa ataatatctg caacacttcc tttataagca
     4801 acccgacctt cgattccttc tggtactaat ttatttgctt cattgacact accttggaag
     4861 taacggtctt ttgaaccttt ttccatcgcg cctaatgagc ccatacctcg gtatgttttg
     4921 aatcgacgtc cttggaagat ttcgaattca cctggtgatt catctgtccc tgccaacata
     4981 cttcctagca tcactgcatg tccgccggca gctaatgctt tgacgatgtc accagaatat
     5041 ttgatccctc cgtctgcaat aatcgctttg ccatattgac gagcaactga tgctgcatca
     5101 taaatagctg tgagctgagg tacacctaca ccggctacca cacgtgtcgt acaaatcgat
     5161 cctggtccga taccaacttt tacgacatct acacctgcat catataaagc ttttgtcgct
     5221 tcagccgtcg caacgtttcc tgcaatcaat gttgcatctg caaaagttga acggatttct
     5281 tgaatttttc ggatcacacc tgcactatgt ccatgagctg tatcgatgat gattgcatct
     5341 gcccctgctt ctaataaagc attcgcacgc tcaaatgtat cgcttgttac accaacggct
     5401 gctgctacaa gcaatcgacc atgttcatct tttgctgcat ttggaaattc gatcactttc
     5461 tcgatgtctt tgatcgtgat caatccgctt aaacgacctt cgttatctac gattggtaat
     5521 ttttcgattt tatgtttttg caaaatcttt tctgcatctt ttagagaggt acctactggg
     5581 gcagtcacta agttgtcttt tgtcatcaca tcagcaatcg gcatgtgata atctgttaca
     5641 aaacgcatgt cgcggtttgt aattatccct accaattttc tgttttcaag tgtttcaacg
     5701 attggcacac cactgatacg atatttgctc atcaattctt ctgcgtctgc tacaagatgc
     5761 tgtggcgtta agaagaacgg atcaatgatt acgccacttt cagaacgttt gacttttctt
     5821 acttcatccg cttgctggct gatcgtcatg tttttatgga tcacacctag tccgccttgt
     5881 cttgccatag caatggccat cttgctgtcg gtaacagtat ccatacttgc actgatgatt
     5941 ggaatattca acttaatatt tttagctaat tgtacgctca tatccacttc gtttggtaac
     6001 acgtgacttt ctgctggtat caataagaca tcatcaaaag taaatccttt tttcgtgaac
     6061 tttgtttccc agttggacat ttttagtacc actcctctaa tttagtttat ctattaaaat
     6121 aattgatttt ttcatttaag tcaatgatct acttggaatc aagttagctt tctcatagaa
     6181 taccaatata aagcgtttac atctgtgtgt attaaaagag attgtaaaaa agctgtcctg
     6241 catcaagtaa taagaacagc caatcaaaaa cagcttctca tgtttttgaa ttggttgggt
     6301 ttattaccgc agatgcaaac ttttgaacac cgtttagtgg agaggctgtg aaaaagctgc
     6361 ttagtttcga gtagtaagaa aaattttgtg aaaatggctt tccacatttt tgcataaatt
     6421 ggacttaata ccgagaaact ggcttttgaa caccgtttat cgtaaaaagg gactgtgaca
     6481 agacttttgt cacagtccct tactattatt taactatgga taaacaaaat gaggttcttt
     6541 actaccccta gatatctagc aaaatagctg atctttttgc tagtttatct tcttgggacg
     6601 gataaacttc caataatccc gtattttttc ttcaagtagt aacggattgc taaagcagtt
     6661 gccccgataa tgataactac tactggatct aatactggat tgataactgg tggcaatagt
     6721 gttgtcgcag aaaacagtac gacccaaaga agtgtcgttc cacctaggat aagcaatgtc
     6781 ttacccattc ctggtttctt gcttcgatct gctccgggat attcatattg atagatgtat
     6841 ttgtacatca tataaaagac ccagccaccg attgcagaac ctagaacgag tgtagaaaag
     6901 ccataagctg gcgttgttcc acgtgaaaac aatccggtaa tcgcaatcat gatacttaat
     6961 agaccaagca acagcaacgt attatctaac cacatcaata aaggggtgga tgaatttttt
     7021 acttctgctg gcttattgat gattgcttcc gttctttcag agactgtccc aaacaattga
     7081 cgggctgttc ttccgccttt ttgttctgca actaaagtag gcagaatgtc atgaagggct
     7141 tttacttttt cttcttcaga caaattcgct gcttctaaag attttttcaa atcaaaaatg
     7201 tactgttgat ttcttttggt taattttgtt tctaaactat tgttttcttt tacgattgct
     7261 cgtaattgtt ctggttccat tatctaatag tcctccttag ccgttccttt tctcaacggt
     7321 cgtcacaaaa gacattctac catattctac agctagtttg atagcccttt tacacattaa
     7381 agcggaataa cattacgtcg ccgtcttgta cgacataatc ttttccttct aatcgaacac
     7441 gtccagcttc ttttgctgca tgcatgtttc cgtaatgatc caagtcttca aaagagacag
     7501 tttctgcacg gataaatccg cgttcgaaat ctgaatggat gattcctgct gcttgtggtg
     7561 ctttgattcc ttttttgaat gtccaagcac gtacttcttg ttccccagca gtaaagtaag
     7621 tagctaagcc taacaaatca taagcagcac ggatcaactg atccaaacct gattcttcga
     7681 ttcctaaagc ttctaaaaat tccgctttgt cttcttcatc taattctgcg atttcttctt
     7741 ctgcacgagc actgaccacg atcacttctg cattttcttc tgcagcaaag ttgcgcactt
     7801 cttgtacata atgattcgcg tctgcatctg ctacgtcatc ttctgagaca ttggctacat
     7861 aaagaattgg ttttgtcgtc aataaaaata gtgatttaac gattttttgt tcttcttcag
     7921 taaattcgat cgttcgggca gattttcctt cttccagtac aggtttgatt ttatccagta
     7981 cagctagttc tgccactgca tctttatctt tggttttagc aacttttgct acacgagtat
     8041 agcgtttgtt gatagattct aagtctgcta acactagttc caagttgatc gtatcaatat
     8101 cttctagtgg atcaacacgt ccttctacgt gagtaatatt atcatcatca aaacagcgaa
     8161 caacgtgaca aatagcgtct acttggcgga tatggcttaa gaattgattt cccaaacctt
     8221 ctcctttact tgcgcctttt acgattccag caatatctgt aaattcaaaa gtcgtcggaa
     8281 cagttttttt cggatgtaca agttctgtta agcgttgcaa acgccagtca ggtacttcta
     8341 ccatccctac gttggggtcg atcgtcgcaa agggatagtt tgctgcttct gctcctgctt
     8401 ttgtgatcgc attaaacaaa gttgattttc ctacgttggg caacccaaca attccagcag
     8461 tcaatgccat aaatttattc actctttctc tgcatttttt tcaattactt tttttaattt
     8521 cttttcaaaa tctcgacggg tcatcatgac gatatgtccg caattcatac atctgatttt
     8581 gatatctgcg cccattcgaa tgacttccca gcgattcgtc tggcaagcgt gaggtttttt
     8641 catttcaacg atatcgtgca agtcgtacat aaaaatagcc tcctttttgt ttattcttca
     8701 tcaaattgaa tattcaagat atcaagaatt cgagtcaaat ctttttgaga aagatattca
     8761 atctcgatct tacctttccc atttttttct tggatagcta cacttgtacc aaatttatcc
     8821 atcaaacgat cttcgctttc tcggatataa tatggttttt cctttgctag ccttgggatt
     8881 tttttctttt cttttccatc attttgattt aactcagaaa caagctgttc cagttggcgg
     8941 acagttaaat tttcagttac tgcacgattg gctaatttca agatcaatgt tttatttttt
     9001 aaacctaaca gcgtacgggc ttgtcccata gataaacgct gatcttgtac catttccttg
     9061 acaagatccg gtaaagaaag caagcgcaaa taattggcga tataaggtct gctctttcct
     9121 aaacgactag ctacttctgc ttgtgttagt ttcaggtttt tcatcagcat ctcatatgct
     9181 tctgcttctt ctaacggatt cagatcttct cgctgcaagt tttccagcac tgctacttgc
     9241 atcatcgctt cttcgtcaaa gtctctaacg atagcaggga tcgtttcttt ttcagctagt
     9301 ttggacgctc gaaaacgtcg ctcccctgca ataatctcat atccttttac tgcggaacgg
     9361 cgtacgatga tgggttgaaa gacacctgat tgttcaatag aattagctag ttcctgcaaa
     9421 gatacttctt caaatgtttt acggggctga tacggatttg gtcgcagctc attcaatggt
     9481 agctggacaa cggtatcatc ttttacatcc accgtctcta agttttctaa atcttgaaac
     9541 agtgcatcga ttcctcggcc taatccttta cttttcttca cgacttacca cttcctttgc
     9601 caatgcttgg tagacttcag ctcctcgaga acgtggatcg tagtcgatga tcggttttcc
     9661 atgactaggt gcttctgata aacgaatatt tcttggaatg atcgtttcgt aaactttttc
     9721 gcggaagtat ttacgtactt cttctacgac ttcatttccc agatttgttc gtgcatcata
     9781 cattgtcagt aagactccct cgatctctaa ttctggatta aagtgttttt ggactaaacg
     9841 aaccgtattc agtaattggc ttaatccttc caatgcgtaa tactcacact gaacaggtat
     9901 caaaatcgaa tcacttgctg taaatgaatt gattgtcaga tggccaagag aaggcgggca
     9961 atcgatcaaa atataatcat actgactgct tacttctgct aaagatcctt ttaatcttga
    10021 ttctcttgcc atcattgacg tcaattcgat ctctgctcct gctaattgga gagtcgcagg
    10081 cacaatactc aaattttcgt gttcagtgat caaagttgcc tcatcaattg gcaattcatt
    10141 gactagaaca tcatagatgt ctcttgtcac atcaggtttt ctgatcccga caccactggt
    10201 agcatttcct tgtgcatcca tatccactag caatactttt ttgccaaggc tagcaagaca
    10261 agcacctaga ttcaccgtcg tcgttgtttt gccgacgcca cctttttggt tcgcaacaga
    10321 tattatttgt gccatctctt ctcctcctat ttgattggct gcttattagg cagtccgggc
    10381 tttcttgggt atttctttgg ggttggtttc tttttttgaa taacgatgat atgccgttga
    10441 tcagagacat tcggcaaggt aaccgcctct tctttgagga atttaccacc taataaagca
    10501 atcgcttgtt ttgcttctgt caattcctct tcactttttg ctgctttcaa tgcataaaaa
    10561 tagccttctt gtttagccaa gggcaaacac aattccgcta acacattcaa acgagcaacc
    10621 gcacgagcag tgacaaggtc aaatgatgca cgaaagtgag gattttgccc aaatgtttct
    10681 gcccggtcat gatataagga agcatcgatt cctaataatc gggttaactc agataagaag
    10741 gttattcgtt tattcagaga atcaacgatc gttatctgca gttctggaaa ggcaattttt
    10801 aaaggtatgc taggaaaccc tgcccccgaa ccaacatcgc ataaagataa aggttgcgta
    10861 aagtctactt caaatgctaa tgtaagtgaa tcgtaaaaat gcttcagata tacgtctttt
    10921 tcatccgtaa tagctgtaag gttcatcttt tcgttccatt cgactaatgt ccgataataa
    10981 atagcgaatt gattcatttg atgatcggta agctctatcc ctttttctgc caaggattgg
    11041 cgaaattctt ctggtatcat aaatattcct ttcattgtga aacagcaatt gtttcacaga
    11101 ttctctccct actttaacgc aaaatctcta gacatgcaat ccttccatat ggattcttaa
    11161 aaatttgaaa agtaagaaaa gccgatagac tttttctttc tagtatgact cataaaatat
    11221 acacagaaga gagaactagc tttgagcaat ttctgcaaaa actagttctc tcttctattt
    11281 attattacgt attacgctgg ttcactgact ttcgcaatct tcccttgttc gatatagacc
    11341 attaggatac ttaggtctgc agggtttacg ccactgattc ggctagcttg ggcaattgtt
    11401 tctggttgga ttttctgcaa tttttgtttt gcttctgttg ctaatccgtt gattgctgcg
    11461 tagtcgatgt tttctggaat ctttttcgct tccatgcgtt ttaatttttc tactttttcc
    11521 atggcttttt tgatgtagcc ttcatatttg atttggattt ctacttgttc gatttctttt
    11581 cgtgtcaatt cttcattttc tggaataaac cgtagaagtt ctgcataagt aatctcagga
    11641 cgtcttaaaa agtcgcttgc tagtacacca tcttttaatg gtgccgcatt aatcgttgct
    11701 aaaaagtctt ggacttcttc tgttggtttg attcgtacat tcatcaaacg tttgatttct
    11761 tgttcaactg cttctttttt cttcaaataa ttcttgtatt gatcttcttt tactaagcct
    11821 acacggtgac ccatttctgt taatcgcaaa tctgcattat catgacgtaa aagcagacgg
    11881 tattcagcac gtgaagtcag caaacgataa ggttcgttcg ttccttttgt taccaaatca
    11941 tcgatcatga caccgatgta accgtcactt cgtttgatca ctaaaggttc ttttccctga
    12001 actttcaaag ctgcattgat accggcaatc agcccttgtc cagccgcttc ttcataaccg
    12061 cttgtaccat ttgtttgtcc agctgtataa agattttcaa cgactttagt ttctaatgtc
    12121 ggacggagct ggtgtggctc tactacgtca tattcgatag catatcctgt acgcatcatt
    12181 tctacttttt ctaatccagc aatagaatga agcatgttga cttgaacatc ctctggtaaa
    12241 gaagtagata aaccttgaac atagacttct tccgtattta agccttctgg ttccaaaaat
    12301 aattgatgac gtggtttgtc tgcaaaacgc acgatcttat cttcgatgga tggacaatag
    12361 cgagcgccta ccccttcaac gatccctgta aacattgggg cacgatgtaa gttgtctcga
    12421 ataatttggt gagtcgtctc acctgtatag gtcagccagc aagattcctg atctagttta
    12481 taggcactat ctggtgtgct gaagctaaaa tgattcggct ctttgtcacc aggctgctct
    12541 tctgtaacag aatagtcgat cgtacttgat tttactcgag gcggtgttcc tgttttgaac
    12601 cgttcgatag caaagccaag ttcttttaga tgattcgcta gtccaataga aggttgagag
    12661 ttattcggac ctgatgaata tttcaattca ccgataatga tttctccgcg aagtgctgtt
    12721 ccggctgtaa taacaactgc tttggcccgg tagctcgctc ctgtggacgt cactactcca
    12781 tgacaaacac catcttcgac gatcagttct tcgacgattc cttgtcttag cgtcaaattt
    12841 ggttcttttt cgatcgtatg tttcatttct gttgaataag catgtttgtc tgcttgtgct
    12901 cgcaatgctc ggacagctgg gccttttcct gtgttcaaca tacgcatttg gatataggtt
    12961 ttatcgatat tcttacccat ttctccgcct aacgcatcga tttctcgaac gacaacgcct
    13021 ttagctggtc cgcctacaga aggattacat ggcatgaatg ctaccatgtc caaatttatt
    13081 gtcagtaata atgtttttga tcccatacgt gcagcagcaa gtgcagcttc tgaaccggcg
    13141 tgtcctgcgc caacaacgat cacgtcatac gcatctgctt cataatgttc caatcgtttg
    13201 ttcctccttt tattttccta aacaaaattg actgaataat tgagtgatta gctcatcttg
    13261 tacactgtcg ccgacaactt ctcctagata gtcccagcaa cgcgtcatat ctatttgaac
    13321 gagatccaca ggcattcctg aatcaatacc tgcaatgact tcttcaagtg agagggaagc
    13381 tttttctaac aacgcgatat ggcgggtatt agaaacataa gtagcgtctc tttctcctgt
    13441 ttctccgctg aagaataaat cagaaatagc tgattccaaa cgatctagtc cgtcgttttt
    13501 agctacggaa attgaaaaaa ctggttcatt ttcgatcaat ttcttcaatt tttcttgttc
    13561 cagctgtgct ggcaaatctg ttttatttag aagaatgatc cgtttcaatc cacttgtcgc
    13621 ttccaataat tgttcatctt ctgctgtcag tggttcgctt tggtttagaa caagtaagat
    13681 caagtctgct tctgctaatg cttttcggct tctttcgacc ccgatccgtt ccaccacatc
    13741 ttctgtttcc cgaattccag ctgtatcgat tagcttgagc ggtacccccc gaacattgac
    13801 atactcttcg attacatctc gagtcgttcc tgcaatatcc gtaacgattg ctttttcttc
    13861 tcttaacaaa tggtttaaca aacttgattt gccgacattt ggccggccga tgattgccgt
    13921 acttaaccct tcccgcagaa ccttcccttg cttggatgta gcaagtagcg cttggattct
    13981 ttgttggatc atctgcgctt tttcaagcaa taacttcgta gtcaattctt cgacatcatc
    14041 atattctgga taatcaatat ttacttctac ttgtgctaat gtctctaaaa tctcttgtct
    14101 caatgagcgg atcaaagcag acagattgcc gtctaactga ttaagagcta gtcccattgc
    14161 tttgtctgtt tttgcccgaa tcaaatccat cacagcttct gcctgtgaaa ggtcaacacg
    14221 accattcaag aatgctcgtt ttgtgaattc gcctggctca gctaatcttg caccttctct
    14281 taacaatagc tgcaaaattt gattgactac aacgatccca ccgtgacaat tgatttctac
    14341 tacatcttcc cgcgtaaatg tctttggtgc tctcatcacg gagaccatga cttcatcgac
    14401 aagctgattg ctcttgggat caacgatatg tccataatgg atcgtatgac ttggtacttc
    14461 tgataatcgt ttgtttccag attgataaac tttatctgct agttgcacag cttgatcgcc
    14521 acttaaacga acgatactga ttgcaccttc tccaggtggg gtagaaattg ccgcaatcgt
    14581 atcaaattct aaagtaattt cagccatttt ttcacttcct ttttcatttt gcgcacaaaa
    14641 aaagtgccca ctccaccctt tatgcacaat aaacggtgaa cttcgcactt tgactgcttg
    14701 ttcttgatta aattctggtc atagattatc acacttgcta taaggaatca agtgtttaaa
    14761 acaattagat gaagttgagc ctgttctgct tccattacct tatatttaat tgtccttgga
    14821 tgtctctttg cgttctttgg tttattcgaa gagaagatac tgtataaaca aggctttatc
    14881 tttcttaaaa aaaacggtac actaaatggt agcgctatct aaactataca cttttgaaag
    14941 gagaaaatat gtgagcccgt tcagatctgc tgcattggaa gaagaaagtg accctcggat
    15001 caacgatgtc gttttatcta cagggaatat caaccagaaa tatatcgtcc gcgatatcgt
    15061 ttttgttgtg gaatgcttag aagctgattt gtttgaccca tcttttccca aagatgacct
    15121 tttcttaaca acgaagagga aattaaagac gaaggtactt gaatatggtg cgaatgcagt
    15181 aatcaactgt cattttgagt atcagcaaaa aaaacgcgac ggaaagtatt ttctggaact
    15241 ttttgcttat ggaacagtga tccaatttat ccaaacaacg attggttaaa tgaaacagac
    15301 aaaaaaagcg gatcaaaact gaagacagtt tcgatccgct tttttctatc aaagttagtg
    15361 actacttcat cgtatactct gataagaatg atttctagta aaatatactt ggtttgaaaa
    15421 actttgtcaa cgtgatttat tcaaaaagta atggaatcaa taatattttt tagcaggttc
    15481 tactactaaa taacgataag gttcctcacc ttccgaatgt gtttttacat gttcatcttt
    15541 gcttaatgca gcatggattt gtttacgttc aaatgcaggc attggttcta agaaaacagg
    15601 ctgccctgtc cgttttactt tttcagccgt gcgatcagct aaacgctgaa tgatctcttg
    15661 gcgcttttca cggtaattgc ctacattgac tactacagac agcttatttc ttgctaaacg
    15721 atggatgaag acttgtgcca gatattgcaa tgcattcagt gtttttccat gtttcccaat
    15781 cagtatgcct tgcttttgtg tttctaaatg gaaaacaacc atattatttg attctctttc
    15841 cattttgaca agagctggag catcaagctg ttttgaaatt tcagtaagat aaactgccaa
    15901 ttccgttaaa gctgcttcat cacctagctc acttacttct tctgtttttt cttcaagaat
    15961 cgtttcgttg actacctcga tctcagctgt tggttcttct tccaaaattt cttctacagt
    16021 ctgttctact gtttcagaaa ctttttcgct gatacttggc tggatcgaaa gccgtgcatt
    16081 ctttcgaccc attcctagga aacctttttt tccttcttcc agtatttcga tttcgacttg
    16141 gtctttggcc aaacccaaat cagccagacc tagttggaca gcttcgtcta ttgttgcagc
    16201 ttcataaatc ggcattagca ttacctcccc tattatttcc gttttttctt aggattcatt
    16261 gctttcttaa gagcacgttc tctttcgcgg attctccgtg cttcttcttc acgctctttt
    16321 cgaattttga aagggttgtt gatcagcagc gtttgtacaa cttggaaggc attggaaact
    16381 acccagtata atgaaagacc gctggctaaa ttgacaccca tcactaaaat cattacaggc
    16441 atcacgaaat tcatgatctt cattgatgca ttcgattcaa tttgactcat gctagataaa
    16501 tacgtacttg caaaagtaaa gattgctgct aaaatcggca aaataaatgt tggatccgcg
    16561 ttccctaagt tcaaccataa gaaatgccct tgtttcaact ctggtacacg tgaaatagat
    16621 tgccataacg ccatcaagat tggcatttgt actaataaag gaagacaacc agcatatgga
    16681 ttgacgccat tttctgcata taaacgctgc tgctcttctc ttaacttact ttgtgtttcc
    16741 ggatctcttg aactatattg ttgctgcagt gcttttaatt tcggttgtaa ttcttgcgtt
    16801 ttacgcatgc tctttgtttg gaaatgcatc aacggcaata ggatcacgcg gatgatcagt
    16861 gtaaataaaa tgatccctat ccctgcatta ccaaatgaca acgccttgat cgcctcagca
    16921 aagtaataaa caatataacg atcccaaatt cctgtactct gtgcgctgac ttccccagtt
    16981 ccgcaaccgg ataagacgat gaccaaacta acgataccgg ccatcaaaag taaacgtttg
    17041 tatttcttca cttactcttt tcctctctgt cgattatttt tgcaagtttt aagacgtgga
    17101 ctaaattgga gcgaacctca tcataagtca accccttcac acttggtcta gcaatcacaa
    17161 taaaatccaa ctccggatca atttcttctt ttatagaata aactgctgct cggatttttc
    17221 gtttgacttc attgcggttt accgcgttgc cgaccttttt tcctacagat aaaccaactc
    17281 gaaaatgagg ctgtcccggt ttttccaaac ggtaaacgac gaatttgcgg ttagcaaaag
    17341 aatctcccgt atggaacacg gcttgaaact ctcgttcttt ttttactcga tacgcttttc
    17401 tcattagttt tctttccttt tctatctctc acttacgctc ataatcatac cataattacg
    17461 attgtccttt catcatttcc ctaaaaaaga gtataaaaaa aaccactgaa accccagtgg
    17521 cctaagctga aagaactttt cttcctttgc gacgacggct agctaacaca cgacgtccat
    17581 ttttagtact catacgttta cggaatccgt gaactttttg acgtttacgt ttatttggtt
    17641 ggtatgttct tttcatatat tccacctcca aaatgttctt atctatttta taaacacaga
    17701 catactttga tagtataacg acaaagtagt gattttgtca atataaaata gaagatcgat
    17761 tgttaaaact tatccacaag attatttttt tctatgggaa tcctatgtgg aaaaagttat
    17821 gcacagggat tatccacaga taataaaagg ctggttttac acagaaaaca cacgtgtaaa
    17881 aaaagtaatc cacaatcagg cgatttcctg tgtattccgt ctcctaacta ttggtatatc
    17941 tgtatttttt atccacagac gcaagaggaa atctattggt ttttacgagt tttccacctg
    18001 tgcattagtg gtgtggaaaa ctttttttga caactgtgtt tttctctttt ttctttttgt
    18061 cgattgtgtg gataattgta attaaaaaat ttttttcttc ggtttctgtg gaaaactatt
    18121 gaaaaaaatg ctaaattagt actagttatt cactttttaa tgaaggagga ggccttatat
    18181 ggtatccctc gatgctttat ggaatgaatt aaaagcaaca taccaaaagg atttatcgcc
    18241 tgctagctat aatacatgga tcgaaacagc gaaccctcga acccttgatc aaaaccagct
    18301 cgttgttgag gttcctagca agattcataa agaatattgg gaaaaaaacc tggcgacaaa
    18361 aatcgttgaa gtcggttata tgttatcagg aaatgagatt atccctcgct ttattactgg
    18421 cgaagaagca gagcaagaag aagttataga agaaaaaaat ccaaaagttg tggcacctag
    18481 tccgctaaaa aaagccatgc tgaaccctaa atataccttt gatacgtttg tcattggtaa
    18541 aggaaaccag atggctcatg cagcagcgct cgttgtagca gaagatccag gttctattta
    18601 taacccgctc ttcttttatg gaggcgtggg acttggtaag actcacttga tgcatgcaat
    18661 tggtcatcaa atgctgcaaa gtcagcctaa tgccaaagta aaatatgtaa gcagtgaaac
    18721 ttttgctaat gatttcatca attcgatcca aaacaaaaca gcagaagagt tccgtcaaga
    18781 atatcgaaat gttgatttac tgctagtgga tgacattcaa ttttttgcag aaaaagaagc
    18841 tacgcaagaa gagtttttcc atactttcaa tgccttgtac aatgaaggga aacaaatcgt
    18901 actaaccagt gatcggttgc caaacgagat tccgaaactg caagaacgcc ttgtttctcg
    18961 ctttgcatgg gggctgtctg ttgatattac accgccggat cttgaaacaa gaacagcgat
    19021 tttacgtaaa aaagcggcgg ctgaacgtct agagatccca gatgacacac taagctacat
    19081 cgcaggacaa attgattcta acattcgtga attagaaggt gcgcttgttc gagtgcaagc
    19141 ttttgctgcg atgaatagtg aagatatttc gactagtcta gctgcagacg cactgaaaac
    19201 gttaaaatca ggaaaaggac atcctcaatt gtcaatcctg cagatccaag aagaagtggc
    19261 aaagtattat cacatccaat tgaaagatct caaaggaaag aaacgcgtca aatctattgt
    19321 tgttcctaga caaatcgcta tgtatttggc acgtgagctc acagataatt ctttaccgaa
    19381 aatcggagct gaattcggtg gcaaggatca tacaacggtt atccatgctc atgaaaaaat
    19441 ccagcagctg atggattcta gtgtcagtat gcaaaatgaa gtatcagaaa tcaaaaactt
    19501 tttactaaat taatttgtcg attctatcaa ctgaaaatag catgtggata aaagaaaaaa
    19561 acgttcaaaa gttatacaca ctttttccac agctgttttt tcgtttctct cttacgtttt
    19621 ttgagttttc cacagaatca acatgcccta ttacttttat tattcttttt aactaataat
    19681 aaataaacaa acattcaaag gagatacatc atgaaagtta ctttaaaccg agctagcttt
    19741 atgcaggaat tgcaaactgt tcaacgagct atttcaagca aaaccacgat ccctattttg
    19801 acaggtgtaa aaatcacact gacacaagaa ggtttgactt tgacggggag caacgctgat
    19861 atatcaattg aaactttttt gtctgttgaa aacgaaaaag caaatatgca aatcgaatct
    19921 actggttcca ttgttttaca agcacgtttc tttagcgaaa tcattcggag acttcctgaa
    19981 gaaacattta ctttagaagt tttagaaaat aaacaagtag cgatcacttc tggaaaagcg
    20041 aattttatcg taaatggatt agatgcagat aactatcctc atcttcctgt tgtcgaaagc
    20101 cataaccaga tgaaattacc tgtacacgta ttgactaaac taatcaacga aacagttttt
    20161 gctgtctcac aacatgagag tcgtccaatc ttgacaggtg tgcatttcat tttatctgat
    20221 aattctttat tagctgtagc tactgattct caccgtctaa gtcaacgcgt gattccagta
    20281 gaacaagcgg ctgatcattt tgatattgtt attcctggaa aaagtttgat cgaattatct
    20341 cgctcattaa caaatgaaga agaaatcgtc gaaatcagca ttatggaaaa ccaagtgttg
    20401 ttcaaaacag aaacgatgta tttctattcc cgtttgctag aaggaaacta tccagatacc
    20461 aatcgtttga ttccttcaag ttttaataca gaggttgaat tttctgttcc tagcttttta
    20521 gcggcgatcg aacgggcttc tttactttct catgaaggcc gtaacaacat cgttcgttta
    20581 tctattcgtc cagatgcggt tgttttatac ggaaattcgc cagaaatcgg gaaagtagaa
    20641 gaaagcttga gttatacagc aagcagtggc gatccattag atatttcttt caatccggat
    20701 tatatgaaag cagcgttgcg tgcatttgga gatatgagca tcaaagtgaa atttatctct
    20761 gctattcgtc catttacatt ggaaccaacg gaagatggcg ttcaatttat tcagctgatt
    20821 actcctgtac gtacaaatta acaatgattt tcaatgttct tgaaaattta attgaaaaga
    20881 aatagaaaaa ataaaatttt aaacagaagg ccctaaaatc tcttttaagg ctttctgttt
    20941 atttttgttt aaatcacctt ttttagatta aatcgtctta tatggaaaaa tatatggata
    21001 aatttgtttt attgcgaaaa aaaaggtata atagagtata cttttaataa tgaaaatgag
    21061 ggatgggcat tgaaacaaca agtcattcta aatacggaat tcatgacgtt gggccaaatg
    21121 ttgaaagaag tcacagttat cggtagtggc ggacaagcaa aatggtattt ggcagaaaac
    21181 actgtgttag tcgatggcga accagaaaat cgtcgtggac gcaaacttta tcccggcatg
    21241 atggttgaag ttccagaagt aggaactttt tttatggtaa aaaaaggaaa cgacgataat
    21301 gaggctgaat aagctgtatt taaaaaatta tcgaaattac gaagagctga acatcgaatt
    21361 ttccaagaac ctcgtcatct ttttaggaga aaatgcgcaa ggaaaaacga atcttttgga
    21421 aagtatctac gtattagcta tgacgcgaag tcatcggaca agtagtgagc aggaattgat
    21481 tggctggact gacgatcagg cgatgattca aggagagatc acaaaaggtt catccactct
    21541 tccattagaa atcttattat ctaaaaaagg ccgaaaaacc aagatcaacc atatcgaaca
    21601 gaaaaagtta agtacttacg tgggacaaat gaatgtcatt ttatttgcac cagaagatct
    21661 ttctttagta aaaggcagcc cgcagatcag gcggaagttt ttagatatgg aattaggcca
    21721 gatcaatcca atctatcttt atgatcttgt ccaataccaa gcaattttga aacaaagaaa
    21781 ccagtacttg aagcaattga atgaaaaaaa acaaactgat ttgctttatt tggatatttt
    21841 gtccgaacaa ctagcagcgt ttggcagcaa agtattgaaa gcaagagcac agttcgtcaa
    21901 gcgattggag ttttgggcta attctttgca tcagcaaatc acccatcaaa aagagcagct
    21961 ggaaatcgag tatctcaccg cagtcgattc tttagaaaca catacgcaag agcaaatcca
    22021 agaacaattt ctcgcacttt tgaaccaaaa taaaaagaaa gacctttttc ggggaacaac
    22081 gacagttggt cctcatcgtg acgatctttc cttttttatt aatcagaaaa atgtgcagac
    22141 ttatggatcg caaggacagc aaagaacaac tgcactaagt gtcaaattag cagaaatcga
    22201 tttgattaaa gaagagacag gggaatatcc aatccttttg ttagatgatg tgatgagcga
    22261 gctggatgat aatcgtcaac tccatctgct agaaacaatc gaaggaaaag ttcaaacgtt
    22321 cttaacaaca acgacacttg accatgtaaa aaataaaatg actgttgaac ctgagatttt
    22381 ttatgttcaa caagggaaac taaaaggaga agaaactgaa tgacagaaga aagaagttta
    22441 gtagaacgcg ctaaagagta tgatgccagt cagattcaag tacttgaagg acttgaagcc
    22501 gttcgtaaac gacctggcat gtatatcggt tcgactagtt ctgagggtct gcatcacctt
    22561 gtttgggaaa ttgtcgataa ttcgatcgat gaagtattag caggttttgc aacgaaaatc
    22621 catgtgatta ttgaaaaaga caacagtatc accgttattg acgacggtcg ggggatacct
    22681 gtagatatcc aggcaaaaac tggacgtcct gcagttgaaa cggtctttac tgttttacat
    22741 gctggaggaa aattcggcgg tggtgggtac aaagtttctg gtggacttca cggagtagga
    22801 tcttctgttg taaacgcgct ttctacacag cttgatgtca aagtatacaa agaaggaaat
    22861 gtgtactatc aagaatatcg tcgtggagct gtagtggacg acttaaagat tatcgaacaa
    22921 acggatcgcc atggaacaac ggttcacttt acgccagacc ctgaaatttt tacagagaca
    22981 actgagtttg atttttcaaa attggctacc cggatccgtg aacttgcttt cttgaaccgc
    23041 gggatgcgta tatcgattga agacaaacga gaagaagaac cagtcatcaa tgaataccat
    23101 tatgacggcg ggatcaaaag ttatgtcgaa tacttgaatg caaacaaaac cgtgattttt
    23161 ccagaacctg tatatttgga aggtgaacag caagatatcg cagttgaagt ctctatgcaa
    23221 tatacagacg gctaccattc caatatcatg agttttgcca acaatatcca tacgtatgaa
    23281 ggtggaacac atgaatcagg attcaagact gctttaactc gtgtgataaa tgattatgcc
    23341 cgaaaacaaa agctgatgaa agaaaatgac gacaacttga ccggtgaaga cgtgcgagaa
    23401 ggattgacag ctgttatttc tatcaagcat cctgaccctc aattcgaagg acaaacaaaa
    23461 acaaaattgg gcaattccga agtacggaca gtcactgatc ggttatttag cgaacatttc
    23521 atgaaattct tgctggaaaa cccaagtgtg ggccggcaaa ttgttgaaaa aggattacta
    23581 gcttccagag ctcgtctagc agcgaaacgt gcgcgtgaag taacgagacg taaaggtgct
    23641 ttagagatta gtaatctgcc aggaaaatta gcggactgct ctagcaacga tcctgaaaaa
    23701 tgcgaattgt tcatcgtcga aggagattcg gccggcggat cagctaagca aggtcgtaat
    23761 cgagagtttc aagccatctt gcctattcgt gggaaaatct tgaatgttga aaaagcaagc
    23821 atggacaaga tcttggcaaa tgaagagatc cggtctttat ttactgctat gggtacaggg
    23881 tttggagaag attttgatgt atcgaaagct cgctaccaca aattagttat catgactgat
    23941 gccgatgtcg acggcgcgca tatccgtaca ttgctgctga ctctttttta tcgttacatg
    24001 cgtcctattg ttgaagctgg atacgtctat attgctcagc cacctttata cggggtaaaa
    24061 caagggaaga atattacgta tgtacaacca ggaaaaaatg cagaagaaga gttgaaacaa
    24121 gtcgttgctt cacttcctgc ctcaccaaaa ccaagcgttc agcgttataa aggacttgga
    24181 gaaatggatg atcatcaact atgggaaaca actatggacc caagcaatcg gatgatggcc
    24241 cgcgtcagtg tggacgatgc tattgcagca gatcagatat ttgaaatgtt gatgggggat
    24301 cgagtagaac cccgtcgtgc attcatcgag gaaaatgcac actatgtgaa gaacttagat
    24361 atttaatcat gaaggaggaa aaagaatgag tgaagaaatc agagaaaaca tccatgatgt
    24421 caatctaacc agcgaaatga aagactcctt catcgattat gcgatgagtg ttattgtcgc
    24481 acgtgcactt cctgatgtca gagatggtct gaaacccgtt catcgccgta ttttatacgg
    24541 catgaacgaa ttaggtgtaa caccggataa agcccataaa aaatctgcca gaatcgtcgg
    24601 agatgtcatg ggtaaatacc atcctcatgg agacattgcg atctatgagt caatggtacg
    24661 gatggcacag ccattcagct accgttatat gctagtggat ggtcatggaa actttggttc
    24721 tgttgacggt gacggtgccg ccgctatgcg ttataccgaa gcacggatga gcaaaatcgc
    24781 tacagaaatg ctgcgtgata tcaataagaa cactgttgat ttccaaagca actatgatga
    24841 tacagaaaaa gaaccagttg tactacctgc tcgcttcccg aatcttttag tgaacggaac
    24901 aacggggatc gccgtaggta tggccacgaa tatcccacct cataacttgt ctgaggtagt
    24961 agcggctatc gatttgctga tggataatcc tgatgtgacg accaatgaat tgatggaagt
    25021 attacctgga ccagattttc caacaggtgg tttggttatg gggaaatcag gaatccgaag
    25081 agcgtatgaa acaggtaagg gttctattac cgtacgtgca aaagttgaga ttactgagat
    25141 gccaaatggt aaagaacgga tcatcgtgac agaattgcct tatatggtca ataaagcaaa
    25201 attgatcgaa cggatttctg aactgcacag agacaagcgt atcgaaggga tcacagattt
    25261 gcgggacgaa tcttcccgtg aaggtatgcg gatcgtcatt gacatccgtc gtgacgccag
    25321 cgcatccgtt atcttgaata acttatataa attgacatcc ttgcaaaatt catttggatt
    25381 caatatgttg gcgatcgaaa aaggtgtgcc gaaagttttg agcttgaaac aaatccttga
    25441 aaactatatc gaacatcaac gcgaagtcat cacacgacgg acggtctttg aaaaagaaaa
    25501 agcagaagca agagcacaca tcttagaagg tttgcgtatt gctttggatc atatcgatga
    25561 aatcattgcg attatccgcg ggtctaaatc agatgacgag gcaaaagcaa caatgatcga
    25621 acggttcgat ttatccgatc gccagtctca agctatttta gatatgcgtt tacgccgatt
    25681 gaccggtttg gaacgggaaa aaatcgaaaa tgaataccaa gaactcttaa aactgattga
    25741 agatttaact gacattctgg caagaccaga acgtgttaca gaaatcatca agaatgaatt
    25801 agcagacttg aatcaacgat ttggtgataa acgacgcaca gaattactag tcggtgaggt
    25861 cttaagtctg gaagatgaag atttaatcga agaagaagaa atcgttatta cattgacaaa
    25921 caatggttac atcaaacgtt tggcaaataa tgaattcaga gctcaacgaa ggggtggacg
    25981 tggtgtccaa ggaatgggta tccacaatga cgacttcgtc aaaaatctgg tttcttgttc
    26041 tacccatgat acattgttat tctttacgaa taacggaaaa gtgtatcggg caaaaggata
    26101 cgaaattcca gaatacgggc gtacagccaa aggaatccca atcatcaatt tactaggtat
    26161 cgattcgagt gaaagcatcc aggcaatcat tgcggtgacg ggagaagcag aagaaggcca
    26221 ctatctcttc tttacgacgc gtcaaggtac agtaaaacgt acttctgtca ctgcttttgc
    26281 taacatccga agtaatggtc tgatcgcaat tggtttgaaa gaagacgatg agctcgtcaa
    26341 tgtgctactg acagatggtc aatctaatat cattattgga acacataacg gctactctgt
    26401 cactttcaaa gaagaagcag tccgcgatat gggacgtaca gcttccgggg tcagagggat
    26461 acgcctgcgt gaggaagatt atgtcgttgg ggcagcctta atgaaggaaa atcaagaagt
    26521 attagtgatc acagaaaaag gttacggaaa acggacaaaa gtatcggaat atccagtgaa
    26581 aggccgcgga ggtaaaggga tcaaaacggc gaacatcact gaaaaaaatg gtccactagc
    26641 tggattgacc actgtttctg gagatgaaga catcatgctg attacagata aaggtgtcat
    26701 tatccgtttc aacgtatcga ctgtttctca aactggacgt tcaacattag gcgtacgttt
    26761 gatgaagatg gaagctgata cgaaagttgt cacgatggca gcagtcgaac cagaagctgc
    26821 agaacaaaca gttgaagaac aaggatcaga agaataacca tcaagactaa gtgtgtaagc
    26881 tagcaagaat aaccgattaa aaaatctggt tattcctgct ggcttttttg attagttgaa
    26941 tttctcttaa aatattcgaa actgttcatt ctgtattgct tttgaccaaa atagagtgta
    27001 taattagagc ttgtgagtag tctaattaat tagataactc tccttgctct taggaaccta
    27061 agagccgaaa gtccacaggg aggtgaatat caatggaaaa cacgaaatat gaaatcatgt
    27121 acattattcg tccaaacatt gatgaagaag caaaaactgc tttaatcgaa cgtttcgatg
    27181 caatcttgaa agataacgga gcagaagtta tcgaatcaaa agattgggaa aaacgccgtt
    27241 tcgcttatga aatgaacggt taccgtgaag gtatctatca catcgttaaa gtatcttctc
    27301 catcatcagc aaatgctgtc aatgaatttg accgtcttgc taaaatcaac gacgatatca
    27361 ttcgtcacat gattgttaaa gaagaagctt aattgttgtt tcatgtgaaa caatagaaaa
    27421 cgatgagagg agatgactat tttgattaat aatgttgtat tagttggacg tctgacgaaa
    27481 gatccagatt tgcgttacac agcaagtggt acggcagtcg caacatttac tttggctgtg
    27541 aatcgtaatt ttacaaacca aagcggcaat cgcgaggctg attttatcaa ctgtgtgatt
    27601 tggcgcaaat cagcagaaac tttagcaaac tatgctcgta aaggaacact tttaggtgtt
    27661 actggacgta tccaaactcg ttcttatgat aatcagcaag gacaacgtgt atacgtgaca
    27721 gaagtagtag ctgacaactt ccaattgctg gaaagtcgtt ctgcatctga aaatcgccaa
    27781 caaagcggcg gattccaaag ttctggtcaa tcagctggcg gatttggcgg aaacaataac
    27841 tctaatcaaa catctcaatc atccaacggt atgcctgatt tcgaccgtga tacttctgac
    27901 ccattcggct caagttcaac gatcgacatt tcagacgatg atttaccatt ctaaacgaat
    27961 ttgataggag ggaaatagaa tggcacaaca aagaagaggc ggacgtaaac gtcgtaaagt
    28021 agactacatt gctgctaacc atattgaata tatcgattat aaagatatcg aattactaaa
    28081 aagatttatt tcagaacgcg gtaaaatctt gccacgtcgt gtaactggta caggcgctaa
    28141 aaaccaacgt aaaattactg ttgcaatcaa acgcgctcgt atcatgggct tgctaccttt
    28201 cgttagcgaa gactaataaa tcctaggaat tgaatgactg atccttttga tcagctattc
    28261 aattcctttt tttctgtaaa aataacctct attcgtttag tcgctcgaat gacgaaagtt
    28321 atggtaaaat caaattagtc atatatggga ggaaaattat ggaaaataaa aacaatcatt
    28381 tatccccgaa acctctcttc cttatcctgt ttatattaat cgaagtattg gtagtcttaa
    28441 ttttaccatt tcgttttgtt agcgcttcgg tcattctatt gatgaattta tggggtgttc
    28501 gctattggtt gaaaatgaaa cgttggctta ttttaaataa acgtgaaaaa atccagcagg
    28561 cttcgactgt agctgaaacc aatctagctt atgtctcaga agtcatgcct gtaggagtga
    28621 ttgcttatca gccatcaagc caaaaaatcg aatggctcaa tccattagca ttgaaaatca
    28681 aagatcaaag tggattaaca gaggaagaat tgctagatac tttttttact gcaaaagaaa
    28741 aaaagaaaaa ccaagtctat ctagcagata aaacatttca gtttgatatg gatacagtaa
    28801 agggtgttgt ttattttaat gatattaccc aaaaccagtt gttaaaactc catcagacga
    28861 acagtcagcc ggtgattggt attttatcta ttgataacta tgcagatgcc attgataaat
    28921 tagatgacaa agagatttct tatttgaact ctttcgtcac tacactagtt tctgactgga
    28981 tgaatgaata ccatgtcttt ttcaaaagaa tcaacgcaga acggtttttc ttcgtagcac
    29041 agatgatcga tctgaaaaag atgatggata aaaaatttga tttgctggat cgctttcgag
    29101 cagaagctga aaaacagagc tcgcctatca cccttagtat ggggattgct tatggctcaa
    29161 gaaccctcga tgaaatcggt ggagaagctc agaataattt agatgttgct ttagtccgtg
    29221 gaggcgacca agttgtcgtg aaagaggtaa aagaaggagc aaaacctgtt tattatgggg
    29281 ggaaatctgc taagaatgtc aaacgcacgc gtgttcgttc tcgagcgatg agtaccgctt
    29341 tgcgtcggat ttttgatgaa acaggcgatg tgtttatcat ggggcacaag tttccggata
    29401 tggatgcttt aggctctgct tttggtgtca gttgtctggc acgcttcaac cagaaaaaag
    29461 cttacatcgt tatcaatgag aacgagatca ttccagatgt agaacggtgt cttgaagaaa
    29521 tccataagca tcctgaatta gaaatgcaac taatctctcc agaacgtgcc atagagctga
    29581 aaaaagaaga ggacctcctc gtgatggtgg attaccataa accttctcta tcgatctctc
    29641 agggcctata tgagcaattt gataagattg tgatcattga tcaccatcgt cgtggggaag
    29701 agtttccaag taaacctctg ctgacttata ttgaatcttc agcttcttca gcttcagagc
    29761 tagtttctga gctgatccaa taccaaagca gcaaaacaaa tcgcttagat cgttttgagg
    29821 ccacattatt gcttgcaggg atggttgtag atacaaatag tttcaatgtc cgtacaactg
    29881 cacgaacttt tgacgttgcc agctatttaa ggtcttgtgg agcagacgca tccttagtac
    29941 aatatttatt gagctcggat ttagcgacat acttggagat cagcaatttg attgctggaa
    30001 gcgactatgt gacttctgat attgttgtgg cggcagggac agacgaaaaa gagtataata
    30061 gtgttatagc agcaaaaaca gcagatacat tgctttcgat gatcaatatc aatgcagctt
    30121 tcgtcattac aaagcggaca gacggcttga ttgggatcag tgccagaagt accggcacga
    30181 tcaacgtcca aatcatcatg gaatccttag gtggcggagg acacttcaca aacgcagcta
    30241 ctcaactgga aaatacgaca gttgaggaaa caaaacagaa gctatttgct gcaatcaaag
    30301 aaaatatgaa tcaaatatat gataaggagt aatccaaatg aaagtaattt ttttacaaga
    30361 tgtaaaagga aaaggtaaaa aaggggacat caaagaagtt ccagcaggtt atgcacaaaa
    30421 ttatctaatc aaaaataact tggcaaaaga agcaacgaaa agcagtatca gtcagttagc
    30481 aggacaaaag aaagcacaag aaaaacacga agctgaaatc ttggaagaag ctaaaaagtt
    30541 aaaagaattc ttggaaaaag aagatactgt agtcgaattg aaggcgaaag caggggaaga
    30601 cagccgtttg tttggttcga tcccttcaaa acaaattgct gaagggttaa acaaacaata
    30661 caaaatcaaa ttagataaac gaaaaatcga attagaaaat ccgatccgtt cactaggcta
    30721 cacaaacgta cctgttaagc ttcatcatga ggtgacggct acgatcaaag ttcatgtcgt
    30781 acaagaataa caaatcttga tagcgatgtt cagctggtaa gtataaccag aaggaaaaaa
    30841 gacaaaccga aatttatctg agaaaacagc tgcagcaatg aaagcaaaag ctgcaaatcg
    30901 atttcagatt gatttcggtt tcttttttgg gaacagcaaa atagcataaa gaaaacgagt
    30961 ataataaaag agaactattt ttttattaca ctttcggtac gaatgatgac ttttctctca
    31021 ttttcttgta aaatgaagca attgaagaca aggagcgata cgtatgaatg aagtgtggca
    31081 ggatcgaatc ccaccgcaaa atattgaagc tgaacaagcg gttttaggct ctgtttttct
    31141 tgacgcagaa acgatcattg atgccatgga gtacatagag ccaaaagact tttatcgtcg
    31201 tggccatcaa attatttttg agacaatgat cgagttaaat gatcgtaatg aagcgattga
    31261 cgtggttact gtcaaagacc ggctggaaca agccaatttg ttagaagatg ccggtggttt
    31321 aagttatctc tcagacttgg cattagcagt tccgacagca gcaaatatcg tctactatgc
    31381 gaaaatcgtt gaagaaaaat cattgctgcg tacattgatc cagacagcaa caggaatcgt
    31441 tacgaaagga ttcgagcaag gagaagaagt tcaaacaatt ttagatgatg ccgaacgcag
    31501 tatcttggag gtttcggaaa aaagaaatcg aagcggcttt ttatctattg ctgaagtact
    31561 gaactccacg attgaaaata tcgatcgact agctcaaaac aatgaagaaa tcacaggtct
    31621 accaactggc tatcaaggtc tggataaaat gacagcaggt ttccagcccg aagaactaat
    31681 cattcttgct gcacgtccag cggtaggtaa aacagcgttt gcattgaata ttgctcaaaa
    31741 tgtaggaaca aaaacagatc gttctgttgc gatatttagt ttggaaatgg gggcagaatc
    31801 tcttgtaaat cggatgcttt gtgcagaagg ttcgattgag gccagccatt tacgtacagg
    31861 tcagctttcg gaagaagaat ggtcgaatct gatcgttgcg atggggagtc tttctcgagc
    31921 aaatattttt atcgatgata caccagggat caaaatctct gaaatccgtg cgaaatgtcg
    31981 gaaactagcg caagaaaaag gcaatctagg cttgatattg atcgattatt tgcagttgat
    32041 tgaaggtaac ggtaaagaga accgtcaaca agaagtatca gatatctctc gccaactgaa
    32101 aaaactggcg aaagagctga aagtacctgt cattgctctt tcacagttat ctcgtggagt
    32161 agaacagcga caagacaaac gaccagtgct aagtgatatt cgtgaatccg gatcaattga
    32221 acaagatgcg gacatcgtag ccttcttgta tcgagatgat tattatgaaa gagaaggcgg
    32281 agatgacgat gatggaaacg agccaaaaaa tgacaacgtc atcgaagtca tcatcgagaa
    32341 gaaccgaagc ggtgcacgag gaacagttga actactgttt atcaaagaat acaataagtt
    32401 ttcgtcgctt tcgccaagag aagaatttta aagaagaggt tgtgagcctt gagcaatccc
    32461 gagagctctc gtaagaagat gcaaggagta accgcaggaa cgactagagt acttaattta
    32521 aacagatctc caaagggatt gtgacaaaag tcttgtcaca gtcccttttt ccgaataaac
    32581 ggtgttcaat cagctgaccc ttggtattag ctcagcaaac gtcaaaatat gaggaactat
    32641 tttgacgttt gtttctctaa tactcaggtc aaaacgctga tcttacgacc tcttttcctt
    32701 aaacggtgtt ctcctgccaa ctcctcggtc acaagtcaca atatcatcaa acatgagaag
    32761 cagttttttg aaattgctcc ttgtgactca gagttaaacg gcaggctcac aacctctttc
    32821 ttaaacggtg ttcaaaagct tgcatctgcg gtaatacgcc caatcaaacg aaaaatatga
    32881 gaagctattt ttgtttggct gttcttatta cttgatgcag gacagctttt tcacaacctc
    32941 tgattcacgg tgttccaaaa gtagcttctt cgaaaaataa gtcgaaattt cacaaaaatt
    33001 tgagaagcaa ttttcgtaca ttccttctta tttcttgaag ctgaccactt ctgttacaac
    33061 ctctttttta ataaccattc taaaaagata aagttcgttt tttaagcgtt tttataaaat
    33121 aaaaataaaa atcattcgat tttatttgaa atatgtatct attattgata caatggttca
    33181 ggattaaata atatagaacg aggtgttcac atgtcatctg tagtagtagt aggtacgcaa
    33241 tggggcgatg aagggaaagg aaaaatcaca gactttctca gtgaaaatgc tgaagtgatt
    33301 gcacggtacc aaggtggcga caatgcaggg cacacaatta agtttaacgg tgtgacttat
    33361 aaactgcatt tgattccttc tgggattttt tataaagaca agatcagtgt gatcggaaac
    33421 ggcgttgtag tcaatcctaa atcattggtt aaagaattag cttatttaca tgaaaaaggt
    33481 gtagaaacaa gtaatttgcg tatttctgac cgcgcgcacg taatcttgcc ttatcatatc
    33541 aaattggacc aattacaaga agatgctaag ggcgaaaata agattggtac aacgattaaa
    33601 ggaatcggac ctgcctatat ggataaagca gctcgtgtag gtatccgtat tgcagactta
    33661 ttagataaag aaatttttga agaacgtctt cgtttgaatc tagatgacaa aaaccgtcaa
    33721 tttgtaaaaa tgtttgattc agaaccactt gattttgacg atatttttga agaatattat
    33781 gaatacggac aacaaatcaa acaatacgtc acagatactt ccgttatctt gaatgatgct
    33841 ttggatgcag gcaaacgcgt gttgtttgaa ggcgcacaag gtgtaatgct ggatatcgac
    33901 cagggaactt atccttttgt tacttcttct aacccagtag ccggtggggt aacaatcgga
    33961 agcggtgtag ggccttcaaa aatcaataaa gttgtcggcg tctgcaaagc ctatacgtca
    34021 cgtgtcggtg atggaccatt tccaactgaa ttatttgatg aaacaggaga acaaatccgc
    34081 gaagtaggac gagaatacgg tacgacaact ggtcgtccgc gtcgtgtagg ctggtttgat
    34141 tctgtagtta tgcgccattc taaacgagtt tcaggtatta ccaacctatc tttaaactcg
    34201 atcgatgtat taagtggatt ggaaacagta aaaatttgta cggcttatga attagatggc
    34261 gaactaatct accactatcc agcaagcttg aaagagttga accgctgcaa accaatctat
    34321 gaagaattgc caggctggtc agaagacatt acaggatgca aaacacttgc agaactgcca
    34381 gaaaacgcac gtaactatgt acgtcgtatc tcagagcttg ttggtgtacg tatctcaaca
    34441 ttttctgtcg gacctgatag aacacaaaca aacatcttag aaagcgtctg ggcgcaaatc
    34501 taaggataaa aacgacgact gaatcaggac gttcttacca gagcgagtcg aagtgtttat
    34561 ctatacgata ataataactt gaagaggtag tgaaagagac gtttagcctt attgccgata
    34621 ggctgtctct tgaacactgt ttattcagaa taagggactg tgacaaaaac cgtgtcacag
    34681 tccctttttc cgaacaaacg gtgttcaaaa gcttgcatct acggcactaa gcccaatcag
    34741 atgaaaaata tggagtgcta tttttatctg attgttctta ctgcttgatg cagagcagct
    34801 ttttcacaac ctctccacta aacggtgttc tcctgccaac tcctcggtca caagtcacaa
    34861 tattcaacaa acatgagaag ctgttttttg aaattgttcc ttgtgactcg gagctaaacg
    34921 gcaggctcac aacctcttgg taaacggtgt tcaatcagct gaccctcggt attagctcaa
    34981 aaaacggcaa aatatgagga actatttttt ccgttttttt ctctaatact caggtcaaaa
    35041 cgctgatttc acaacctctg attcacggtg ttccagaagt agcttctacg gaaataagcc
    35101 gaaatttcac aaaaatttga gaagcaattt tcgtaaattc cttcttattt cttgaagctg
    35161 accacttctg tcacaacctc ttattttgat tagaacttgc gaaaataaac cggttaaagt
    35221 gtgtctagcg caagagattt gctataatag acgataaggg taaaatgtaa cgagaagggg
    35281 ttgaaaaatg tgtatcaaat tgtaacagat tcttgttgtg atctgccata ccatgtatta
    35341 gcaaaagaaa aagttgattt tttatcaatg aatctccaat tgaatgatca agagttagta
    35401 gatgacctcg gaaagacatt caattatgac cgctacatga atcagctgaa aaatggtgcg
    35461 atgcctacga catcacaaat caatgttggc cgatatttag aatttttccg ttcttatgcg
    35521 gaaaaacaga ttcctgtttt atatgtggct ttttccagtg gattaagcgg ctcttacagt
    35581 agtgcgcttc aagcggttga attgctgaag gaagaatatg aagaccctga gatctatgtt
    35641 tttgatacaa aagctgcaag tattggggaa gggcttttag tattggaagc tgtaagattg
    35701 aaaaaagaag gtaaaagcat cgaagaaacc ctagagtggc tggaaagtaa ttatttgaaa
    35761 gtccattcat gggtaacagt agacgatctg aaatatctag aacgaggcgg taggatttcc
    35821 aaagcaagtg ctactcttgg cagtctattg agtgtaaagc ctattattac ggtggataaa
    35881 gaaggaaggc tgacgaatgt tgacaaagtt cgaggaagaa acaagtcgat ccagacaatc
    35941 gtcaatgaga cggtaaaaac aatcgttgca ccacttgatc aaatcgttta tgtggcttac
    36001 gctggggatc tagagagcgc ggagaaggca aaacggcttt tggaagagca gataaagatg
    36061 aaagatgtga agctttatcc cttaggacca acgatcgcca gtcacacagg ctatggctgt
    36121 atcgcgattt tttcgatggg aatcagtcga taaaagcagt aactcattct gtaaaaaagc
    36181 ccagaaattg attgtaacaa aaacagcgac tcttaaaaga tagatgagca tcctatctct
    36241 tagaggtcgc tgttttttta ttttttaagc taaataggcc attatgccca taactcgttc
    36301 aattgttttt gaatatctgc attttccaaa aattcatcat atgtcgtatc agctcgatcg
    36361 atcacacctt tatccgaaac ggcaatgatg cggtttgcca atgtttggat aaattgatgg
    36421 tcatgtgacg caaataagat tgagccagta aatgccatca agccgtcatt caatgctgtg
    36481 attgattcta agtctaaatg gttcgttgga tcatctaata caagtacgtt agctttcgaa
    36541 agcatcattt ttgaaagcat gcaacggact ttctctccac cagataaaac attcactggt
    36601 ttcaatactt cttcaccaga aaacaacatt cttcctaaga agcttcttaa gaaggtgtta
    36661 tcatcttctt ctttacttgc atattgacgc aaccagtcta aaatcgttag atcagtatcg
    36721 aattctttcg ttgtatcctt tggtaaataa gaattacttg ttgtcacacc ccaacgtact
    36781 gttccagtat ctggcgtaat ttcacccatg ataactttga atagcgttgt tgttgttata
    36841 tcattttctg cgacgaaagc gaccttgtct tctttgctca aattaaaaga tatatcatct
    36901 aaaattttct ttccatcaat cgttacactt acattttcta cctgcaataa gtcgtttcca
    36961 atctcacgtt caggtttgaa tccaacgaat ggataacgtc tagaagaagg ttggatatcg
    37021 tctaaagtaa ttttttcaag cattttttta cgtgaagttg cttgttttga ttttgatgca
    37081 ttcgcactaa agcgggcaat aaattcttgg agttctttga tttgttcttc ttttttggca
    37141 tttgcgtttg cttgaagttt agccgctagt tgactagatt ccaaccaaaa atcataattt
    37201 cctacataaa gtttgatttt accaaaatct agatccgcca tatgtgtaca tactttgttc
    37261 aaaaagtgac ggtcatggga tacaacgatc acagtatttt caaaattgat caaaaattct
    37321 tccagccaat tgatcgattg gatatccaga ccgtttgttg gttcgtctag caaaagaaca
    37381 tcaggtttac caaatagtgt ttgtgctaat aacactttta ccttttgtcc accagttaat
    37441 tcactcattt gctgatggtg caattcttca gggatgttca gaccttgcag taaaacagct
    37501 gcttctggtt cagcttccca tccattaagc tcagcaaatt ctccttctaa ttcagctgct
    37561 cgaataccat cttcgtcagt gaaatcctct ttcatgtaaa tcgcatcttt ttctttcagc
    37621 acttcataca gacgcttgtg tcccatgatg actgtttcaa gtacagtata gtcttcatag
    37681 tcgtaatggt tctgtttcaa tacggccatc cgttcatctg gtcccatagt aacggaacct
    37741 gtggaaggct gcagatcccc tgataacact ttaaggaacg ttgatttacc tgcaccattt
    37801 gcaccaatca agccatagca gtttccgggg gtaaatttaa tattcacatc atcaaatagt
    37861 ttgcggtctg aaaagtgcaa actcacatca tttacagtaa tcaatttttt tcctcacttt
    37921 tatcattatt agttttgaca agtcttatag aattatatag gggaaaggta aaagattcaa
    37981 ggcaatcgaa cattctttgt gtttcacgtg aaacagaatg aaaaaaataa aaataaaagc
    38041 ggagcgaaaa tagctactat aggatttatt agtttgcaac agcataattt ttgacgttat
    38101 ttataagagg gagtactata aaactgtaaa agaaatgaaa gaaggattga aaatggatga
    38161 aaaaagaacc atcgactggg gctcattgat tttgggaatt ttatttgtac ttgtctcatt
    38221 gttatcattt caagaccctg tcggaaattt agttgcaatt gtagtagtct ttgcgatttt
    38281 tgcctttatt aaagggatat ttgaactgtt tgtacggaac agattaaaag agctaacagg
    38341 atataaagga aagatgccct taataatcgg gatcatcgat attctagttg gcgtcttttt
    38401 cttattcaat attggtgcag gagtcgcagc attgcctttt gtttttgcag tatggtttat
    38461 tgctgattca gttttagcgt tatttactgc tgacttggca agaggagtaa gtgaaggata
    38521 ttattggttt acgatcatcg tcaatatttt aggaatttta ttaggaattt tcttattgtt
    38581 taatccaatt tcttctgcgt tgacattaag ctttttagtt ggattctatt ttatgttatt
    38641 tggtatcaca catatcgtct atgcatttag ataagtaaaa tcaaacaaat aaaaaagccg
    38701 gcttccgatt acagttttac tgttgtaatt ggaagccggc ttttttatca tggacaaata
    38761 tgcacgaggt gatttttagg tgtcgagaca aatttatgca aaaatgtgag aagctatata
    38821 cacaaatttt atcttaatac tcgaagcata gcgcagttgt cacaacctct tttcatataa
    38881 ttcattcaat actggcaaat catggacttc atatttttct ttgaatgctt ggatttcttc
    38941 ttcagaatat tttcttggat ctaaatgcag atcttctact ggcaatgggc gcttgttatt
    39001 gatcttcaca tcgattacta caggacgatc tgattttgca gctgcatcaa aggcaaatcg
    39061 aagttgcgag tattctgtga ctgtaaagct atttgccccg agcgcttctc caactttacc
    39121 gaaatctgca tcttctaaaa atacaccaaa tttcttttgt tccgtatctt cttgttctgc
    39181 ttcgatgaaa ccaaaagaat cattcgaaaa gacaacatta ataattggaa gctgatattt
    39241 gacttgtgta atgatatctt gcatgaccat agcaaacccg ccatcaccac ttaaggtaaa
    39301 tacttgtttt tctgggtagc ttagttgtgc ggcaattcct cctggcacac cattacccat
    39361 agttgcgaac catccagatg tcgtatgttt ttgtttaccg ttcatattca ataaacgaat
    39421 agaatggata gtcgtgttac cgacatctgt gacgaaaatc gcatcgtctt cagcaatacg
    39481 attgatttct ttatagacag cctctggacg aatcggttct tcctctcgat cttcaaaaga
    39541 tttcaaccaa tttacccatt tttctttatt ttcttgattt gctaggtacc agctatcagc
    39601 agggcgagca tcccctaact cgacaagtct ttgcaaagct gtgtttgcat caccgaggat
    39661 cgacaaagat gtttcatggc gtcgtccaaa ttttgacgca tcgatatcga tttggataaa
    39721 ttcagcagtt ggattaaaaa atgcacgtcc aaatgggaag tcgctaccga caaataagat
    39781 cagatcggct tctgccaatg cttcatttcc gggtttagta gcgacacgtc cagcaaaacc
    39841 aagaaagttt tcataatcat caggaataat tccttttgct agaacggaag cgacaacagg
    39901 aactgaaaaa tgttctgaga attctttgat ttgcggaaat ccgtttcgcg ttccttgacc
    39961 aacataaaga actggttttt ttgctttttc aagataaggc aaagcagcta gcaaatcttt
    40021 ttcttctggt aatatcacac ttgttttgtg agtgtgagcg gtggagaaag gttgttcgtc
    40081 aatttcttca aaaccaagat caacagggat agtcacgata gccacgcctt tatgctcata
    40141 ggcggcttta atggcttcat ctactacatg cggaagactt tgtggtgtca tgactgtacg
    40201 attgtatacg ctaacatctg cgaaaatagg attttcattt agttcctgga aagagttata
    40261 gttcatggat gtagaagcaa cttgtcctaa gaacgctact agaggcacat ggtccatctg
    40321 tgcatcatac aaaccattga tcaaatgtgt cgcaccaggt cctgcagagc cgaagacagc
    40381 gccgactttc cccgttaatt ttgcatctgc agcggctgcg agtgcgccga cttcttcatg
    40441 acgaacttga atgtagttga tttcggtttt ttcttgatat agcgcatcca taattgaatt
    40501 gaaagatcct ccgggaatgc cgtaaatatg gtcgataccc cagctttcaa gaacttttac
    40561 cattgcgatt cctgcattga tttttgacat ggtgtttcac tcctttagca atgatgatac
    40621 ataaattttt tgtatctaga gtatagaact ttttatcgaa aatcccaact aattcgtctt
    40681 ttcacaaaaa tgtagtcatg tttttgggaa tatgctagag tagtacatag atagattcaa
    40741 gagaggggaa tggcatggtt gaatggctat tgagacttga accttggcag caagcacttg
    40801 ctgggacagc ttttacttat tttatgacag ctcttggtgc cgggttggtt tttttcttta
    40861 aagaaatcaa aaaagaagtg ctaaacttga tgttaggatt tgcttctggt gtcatgattg
    40921 ctgcaagttt ttggtctttg ttagatccgg ctattacaaa agcagaagaa aatggagata
    40981 ttgcttggct agttgtaagt attgggtttg gtttaggcgg tgtgttttta tatatggcag
    41041 ataaaacact gccccatatg cattttggac cacaacatga agcagaagga ttgcctaccc
    41101 atttaaaacg gacgatcttg cttgtttttt ctattacatt gcataacatt cctgaaggtt
    41161 tagccgtggg ggtagcattc ggtgcagcag caactgcgga taatccgaca gccgctatat
    41221 tagccgctgt ttcggtggcg ctaggtattg gtatccaaaa cttcccagaa ggagcagctg
    41281 tatcaatccc gttgcgacaa gaaggtctga gtcgtaaaaa ggcctttgtt tacggacaag
    41341 cttctggaat cgttgaacca attgctggag tgattggagc gctactagta acgaaagtgg
    41401 agctgctgct tccttatgca cttgcttttg ccgcaggagc aatgatttat gtcgtggtgg
    41461 aagaattgat ccctgaagca cagcagacat tgtccagcaa acgtcatttt gcagtttttg
    41521 gcgtcatgag tggatttatc attatgatga ttttggatgt ggcattagga taaataaaaa
    41581 aaatatatat gatatatcaa atagggccgc gaacttatat cgcgacccta tttttttagt
    41641 aagttatatc ataatgaagt gaatggtatc atacattaat aatagttttt tctatgtgta
    41701 tttcctttga tccagcgaac aagccagatg ataagtgcga ttgggattag aactttgacc
    41761 gcaaaccata aaagcccgct taggacactg acaaaaagac tgatgacgaa gctagctgca
    41821 attagcacta aaagaaccat gaagatattt aagaacatat ttttttgacg catacaaaac
    41881 cctcctcttg ttcattcttt actatagaaa aagtataaca gggattgttt aaaaacacct
    41941 ccaactgaag actgattttt acttcatcta cttaaatgat caaacaattg ctttttctct
    42001 ggtttatcat ctaaaataga gcggaaaagg aggagaatat catgcctaaa aatcgtcttg
    42061 aagcatttac agatgctgta atcgcgatta tcatgactat tttagtttta gaacttcatg
    42121 aacctaacgg agacacgtgg gaggcgctcg ctgcattagg ttaccgattt tttatctata
    42181 ttgtcagttt tatcatttta gcgatttatt ggaacaatca tcatcattta tttcaagttg
    42241 tacataagat aaatggacgg gtcttatggg cgaacagttt ctttgttttt gcgctttcgc
    42301 tttttccgtt tgcaacttca tggatcagtg agtacatcaa tagcctggct ccagaaatga
    42361 cttatggttt tgttaccttg accgctaata ttgcgtatta tatcttggca aaagaacttg
    42421 tcaaagcaaa taaaggccac gagaaagcat tgaaggcgat gttgaataat tacaagaaat
    42481 cgtatctatc gattggtttg aatgtgttag gaattgtctt agggtattta gtagcgccag
    42541 tagccgtgct gatcgcaaac atcgtgattc tagctgcttg gatcatccca agtcgtacca
    42601 tcgaatcaca atatcggtaa aatgatccgt gtttaaaatt taagagaaaa atatagaaaa
    42661 aataaataaa cgatcaaata tccgagtgat actagtattc aaagagggat gcagatggat
    42721 caaaaaagaa ttcatgtagg actgattact agttttgtca cgatgcttat ttatttggat
    42781 caaggtcatt tgacttggac agtagttggt gtcgtaggat ttttgacatt attcacagca
    42841 ataaaaaaat aaacatcttt atgtaatctg atgttctatc tatttaagaa aagcatgttt
    42901 acagcagtat gattctgttg taaacgtgtt tttatttgtg gaaaatttaa tttttagaaa
    42961 agtgtatcga aaaatagata gttagttttt ccacagtgga taacttcgta agagataaaa
    43021 ataaaaaatt agtcaaagaa gggcgtatag atttcttttg ataggagata tacaacagaa
    43081 atgaataaat attaacgtgt tttcaaacag tataaaacgg tgtggttttt ggaataaaaa
    43141 aataacatat ctcattttac acttttgtta ttttatactt aaaaaataac gacacagaaa
    43201 ataccgtgtc tcagatttct ctactacttc taaaaactac tgtttttttc ctttgaaatg
    43261 ccttcaaaaa gtaggatcag gcagaatata ctggcagaat caaggagaag ggagcctaaa
    43321 taaggagaaa aaccatagaa aatataaagt aattacttat aaaacaagct ttttatagaa
    43381 aaataatata cctgagtagg gtataaatta agacggaatt cctttctaga ttgagatatt
    43441 gactaggaag aatgttaaga ttttaagcct ttaagctaaa aaaaaaccct ttttgttaaa
    43501 taaggggaat atagcctgtc agtttcaaac tgcaagtcgt cctgctgcaa taatatagta
    43561 gattagagat gaaaatagat ttttttaaac caatgactga ataaaaagac cgtcatagat
    43621 acacattgga atgtgtctat tacggtcttt ttttacgaac gattttattg aagacggaca
    43681 gttcgggtca aaaataaaaa agctacgatc agaaataaga ataaaatgta tcccaaaata
    43741 atggctagac ggtctaactt atttgtcgat agatgtttgt ttagaagttg ataggcccat
    43801 aaacctatca gtcctcctgt tgtattagtt atcaaatcag tgatgtccgc tacgccaata
    43861 ccgaatagga attgaatcaa ttctgccaag aagcttacaa ggaaaataat ccctaatttt
    43921 ttagaaaaag aaagtctctt gaaattgaca ttcaaaagca ccccaaaagg aaaaaagaca
    43981 atcaagttat aagtggtctc acgtaatacc gtcgtatttt caaaagaaaa agtagaaaaa
    44041 gggacgagat tgacattgct gtaagaataa gtgagaaatt gtggaagatt aaatgatagc
    44101 ttgaataaaa tcaaccataa tagaaagaat aaataaatcc ctaataaaaa ttttgataca
    44161 taccgcatag acatctcctc catttaaaaa gtataccatc gaaaaccgct gaaaaatcat
    44221 aaaaaagtat gaagtttaag gaccaacgta gttatcgtac gaaaattaga agcttttttt
    44281 tgttgggaat ccggtttctt taacaaagct aagatagcga gttgcttgga aaagttgcta
    44341 tcttttttct gtcaaaacga ctcggaatgt tcgttttttg ttctaatttc tccaagttgt
    44401 ttactttttg atactaagtt tatgaaaaat caagaaatag ttgttatact aggtaatgct
    44461 gacaaacggg attggcattt catcttggtt cagctttttt tccaattact aggaaggagg
    44521 gatagattgt tcaagactta ttttcgtttc aataaaatac aagataatcc ttttcgtatc
    44581 atgaatctga tgctacttat ctttattgta ttatggttag gttacataaa tcatgatttt
    44641 ttgatttcaa gtttttcatc attagggatc tttacttttt tctattatca aaacataccg
    44701 atgaaatatt tgctgaaacg tatgtttctg atcggttgcg gactgttggt tgctttcgtt
    44761 ttggggatct tatcgactta tgttttatgg cttgaaccat ttgccgtagc tttggttgct
    44821 ttttccagca gattcgtgtt gcgattattc catatttcta aaccaggtgg cttgtttttt
    44881 gcgatgcttt ctgccatggg aacgagcatg cagctgccaa tcgctcaatt accgatcgtc
    44941 tcactttatt tttttatggg tgtcgttttt gccctgattg cggcagtgat tacaaaatta
    45001 ctggattcac ggcctgagca gacaattgaa aaagcaactt taaaagaaag atttcacgaa
    45061 gagcctttag tgatcattga ttctgttttc tacagcgctg ccttatttct ttccgtttat
    45121 gtcagtcacg gcttgaatct gcataatcct tattggttga ccttgtcatg tgcatcgatt
    45181 ttgctagcag aaaacttgga tgcgatgaag catcgacaag tccaatatct gattggatcg
    45241 atgggtgggt tatgtgtgtc ggcattcttg tcatttgtgc cgtttacgca gttgcagacc
    45301 atttttctga tcacttttct atatggaatc gcacaatttc tagttgctag aaattatgca
    45361 gttgctaata tttttcttaa tctgatggcg ttgatgctat ctacacttat tcgtggagca
    45421 tacttgattt ctttgatcga atatcgtttt ttagggatcg tcataggaag tttcatcggt
    45481 ttaggtgttg cgtgggtaat gacggttggt ttgcaacatt atttaacggt tgttcgtcaa
    45541 aaattagaat agaaggacaa atatatgtgc caaaagaggt tatgacaaga tttttgtcac
    45601 aacctctaat tcaaggtgtt caaaagccag attctcagca ttaagtcaaa ttcacacaaa
    45661 aatgtggaaa ggaactttca aaaaaatttt atatttattc tatcaaaaaa ttttgtttat
    45721 gatggtaact gaataaactt tcctgagatg gtcaatgctt tccttgaaag tcttacagat
    45781 attctttctt catagaaagt ttaagtttta tagttagtgt acaataaaag tatgtttata
    45841 ccatatggtg tccggataat cagtcattta aacaatgagg ttcaaagata tgaaaagggg
    45901 aaatttgatg aagaaaataa gcaatgaaat ccaaattctg gcagaaactt taactcttcc
    45961 caaagtcgtt atagagcaaa tgattgtaca agcaaaaaac atccttgatg aggtgtttga
    46021 gcaatatagt catgaactaa tggatcctaa taaagcaaag gcgactgcaa agaaaataga
    46081 caaagtctat gaagaggaac cttttgcagc attagctatt tatctatatg cagcttctca
    46141 aagttggaaa aaaatttatc aaccattgaa gattccaaga actatctatt tggcaacaat
    46201 gaatgctttt actagattta ttcatgaaca tttccaagta acaggaagct atcgctttga
    46261 ccgtggtttt tggatttggc attacactag tggcttgatc tttcggattg gtgaattgga
    46321 gtacgaaatg acatattttc cgaaagggta cacggatcta agattagaag gaaagcagtg
    46381 cctttctctt catattcctt ccgatgcaga tttatctcag aataagataa gagatagtta
    46441 cgaaagtgcc attcgttttt ttgaacttta ttttcctgat taccattacc aagtgatgta
    46501 cacagatact tggttactat cacccaatct tactaaatgg ttgaaaaaag agtcaaaaat
    46561 ctgtcttttt gcagccgatt atcgattatt atcagttgat gaacaggacg atagtggagt
    46621 cccttggatc tttggacgag tagatgccca aatacatgat tatccagaaa gtacgagttt
    46681 acagcgacaa gcaaaagagc aattgcttgc aggcgagcac attggctctg gcttaggtat
    46741 tcttacttca taagtttagt acttcgaaat agtttaaaag agcgtgagac aaaagtaaag
    46801 aacacttttg tctcacgctc taaacacgta taaacggcgg aagcagaagc aactccttcg
    46861 gaaataagct gaaattcaca aaaatttgaa aagcaatttt cgtgaatttc ttcttatttc
    46921 ttggagttaa acgcttctgt cccagcctct tttttagtgt tctatcgaac acacagtacg
    46981 ttcaataggt aaactagcta taacaataga tattaaagtt aacggacaag agggcctttt
    47041 ttaggtagct aaaacgtaat aatgaaaatt tatttccaga gataggagtc aatcagctag
    47101 tctgatcgac tgttttaaca agttgacttt cattttaact tttgatacca tttaaaacac
    47161 aaatcgtaat tgtttcgatt tgtgtttttt agcatgacta caatcaaaga agaaagtggg
    47221 tcgtccatat ggaaaaaaca gatatctcaa gtgcataccg ccgcttgaaa agcccaaata
    47281 tcaaaacacg aaaacgtgcg ctgaagatca tcaaagatgt gaaacggaat agtgggaaaa
    47341 ggtaagaaga aggaaactta attaaaaaaa tagccgaaaa acgtttttcg gctattttca
    47401 ataatcggtt tttgtgtaag gtttccgaaa gaactgctgt tgattttcca tttttaacat
    47461 atgataaatt ggtagaatct ttcttttcaa attgatacca ttccctaatt ccagttatat
    47521 agttaactac agaaccacaa agcacgtacg tttataaaag tattatctgt agttattttt
    47581 caattccctt tcaacctctt attttgacca gtagtattga tcactttacc ttttaacacc
    47641 tttgttcgtt tttgaaacat ttttagtcaa gaatggaaac gtaactacat aatcaaaggt
    47701 tttgcgaaat atttcatgaa atatttcatg tagaagcgag caatacggtt gcaaataacc
    47761 attatcgttt aataagcagt tacgaaagct cccaatatct ctaacgttca gctcactcca
    47821 tttcttttct tcaaaaaaat cagtgattcg tatctctttc tatttgtcga aaaagttaac
    47881 tttaaaaagt aacgccgaaa aaatatgcaa aaaaataaaa aagcagttac tattgctaga
    47941 ataacatcaa ggaataataa gttttggttt tcagataata gtcgataagt tgtgtattgg
    48001 aattttagat acagtaataa agtatttttc atgaaatttt gtgagaatca ctattacaag
    48061 tatctttaat aaaattaatg gttaaggagg aacttaaagt agaatgtaaa tgaatagttg
    48121 tagtcagtcg attgtgatac tagactcaaa gttgcaggaa taagtttttc aatttgtgat
    48181 tggaagataa aaaatgaaaa agaagggctt ttttttatac tttgttgatt gtagtgttta
    48241 ttttccctct gtttagccaa gtaacatttt ctatgactca accagtttca agtgaggaac
    48301 atgataacct ttttcgtgct acagaagagt ttaagaaata tcaggaaaat attgttccgg
    48361 agactccatt agctagtgag ccaattactg atattgatgg aaaccaaata ggatggtata
    48421 tacagtacga agttatggtg gtaaataatg actcaatagt aaaaaataca gtaggcttag
    48481 agctaacacc gattgaaaag gtgagttcat tgttaacgta tatttatact gatgaaagtg
    48541 acgagactga gatcatttta acggattact caaaaattgc tactgataaa aagtatatat
    48601 tgtggatgta gacactagtg aaatacttta tgatatagaa attgatgaaa atttactaga
    48661 tgtagcacaa gaaatcgatc atcaaaagca ggaagctatt cgatatgctg gtagaaaagg
    48721 gaaaagaaaa tatttattac gtgaatgtag caaaagttag agaagatgaa aatgagtgga
    48781 aagaattcaa gagtcgctac tcaattaact cgactccgac atttacagtt tacagagaag
    48841 gatcgataga aaaaactgtt ttttggacta aagaaagtgg aatgtcttta gctgaagtag
    48901 aagagttttt ggattatgtt agtatgcaac agtaagggag ttcttattat gaaaaatttt
    48961 gacacatatt atgttattgt tttttcgata tctttgctgt taatattact aactttaatg
    49021 cagtataaag agtatccaga gtattttatt ttaacttttg tatatttagt aagtagctac
    49081 acacttctat ataaattcag aagttcttct agagtttcta aagtttgttt cttaattagc
    49141 ttattgattc tataatattt ttgatttata taggaaatta aaaatattat agaatcaaca
    49201 ctcagagaac gggttggttt ttaatagatt taataagtac tgataatgaa atttctcctg
    49261 gttgggaaaa cgaagttggt taccctgtag aagaattttt tcaagattac tttgcggagt
    49321 atgccgatag gataatgaga gattacgtaa cagataaaac tcaagaagtc atcaatcaat
    49381 cccagtcatt aaatgaatta tctgataagg cgaaagaaga agcagttgcc attatttctt
    49441 cagggattag tgactatatt gatatgagtg gtgaaatcaa ttatggcgat aaagacaaaa
    49501 ttgtacgcat gataaaagct tttatcaaaa cttttgatgt gaaggcagat cttaagttta
    49561 atgatactta cctagtagga aaattaatta atatccttgg gacagagaaa gatacggaaa
    49621 cgtttatttc ctctttgtct atgttgttta cagataagca tttttcaact cagcttggtg
    49681 gagaggaact agtggaaatc ttttaaccat tacgcccagc attaataaaa gtatatcaaa
    49741 aaagtactcc tgatgatttt taagattggt ttgagaaata atgtgaaaac actattttta
    49801 atgttaatta tagatagaga tgaacgcgtc atatggttaa aagaatgaaa aagtcaaaga
    49861 tacactgcta tatttcacta aggatatagc agtgtatctt tcatgtcgtt tcactggcag
    49921 ttttttataa aaggaggtgt gcatttcagt tgcttttctt tggtagttag ttgaatcgtt
    49981 taagaattaa cggatgtggc agacaggtgc tctgtccaaa aaccagtaaa atctttgatg
    50041 gttccatctt tatttagctg tgcctgttca aatactgttt tgtttttacc gaatggatca
    50101 attgcaataa ttgttccgat ccccgcacgt ttggcagcaa gtaatcctga gtaagcatct
    50161 tcgataacaa gacaatcagc cggagctaag ctcagtttgt ctgccgcttt aagaaaaata
    50221 tccggttgcg gtttcccagg gaaagagccg tcgtcataga caatcttgtc ccagtcaaac
    50281 cagcgggcta aatcgaaaat atcaaaataa aaagcaacat tctctttgac agtagccgtg
    50341 gcaatcgtta atggaatctg ttggtcagtc agctggtcaa gagtcgcttc aagccctttt
    50401 gtcaactgaa gctcgttttc attttgcaag caaagcatac gataatagct ttctttttct
    50461 tctgacaata cggcgatctc agaggcggtc aaatgctctg aaatgaaatg tttcaaaatc
    50521 tccgtattcg tccgaccatg aatatttttt aaaatatcct cgtctgataa accatctttt
    50581 gtatgatcat gtatcatatg cagccaagca gcttcatgca gatgagagtc caaaaacatt
    50641 gttccgttga aatcaaatat aatccctttc atagttacgc tcctatagtg gtattttcgg
    50701 ttgaatcagc aatttgcaga tcgacatatt gtgcatagtg ctcttgaacc tcccgcgaga
    50761 tctggcgatc cgtaatgagc agatcgaaat catgcaaatt ataaaacaca taaaaatcat
    50821 ctcgattgaa ttttttatta tcaacgagta gatatttctt acgggaatta tttagagcga
    50881 tctgctgtgc ttctccttct tcggtactgt aagtagagat cgcttcgttg tgaacgccat
    50941 ttgaactgat aaatgctttt gtaaacttta gtcttcttaa attgtcgttg gtgatcggtc
    51001 cgacaaatgc gccggtattt ttccggaaat caccgccaat cagtagaatt tctggtccat
    51061 ctgcttgagc aagaatatca aaaactggat aattatttgt aatgatccga attttcttgt
    51121 tcagtaaatg cgtcgccaat agttcgattg ttgtacctgg tcctaagaaa atcgtttctc
    51181 cctcgtgaat caaggaggct gctgtggcag caatttcttt tttttcttga atctgcagag
    51241 tggctttttc agtgtgagaa agttcttgat ctaaagtgta ggaaagactt tgagcccctc
    51301 catggacacg cagcagcttg cccgattttt ctaattcatc taaatctctg cgaacggtca
    51361 tatcagaaac atctaattga ttgatgattt cattaacggt aaggattcct tttttattga
    51421 ccatttctac gatagtaagc aatctttctt ttttcaacat tgtcttcacc tctaatttaa
    51481 ggataatcta tcatataaac ggacaacagt caatctaatt cgaacaaaag taaacattta
    51541 gggtggtttt tatgtttgtt acaatagatt ttattgctgt tttctggttt ttatgattat
    51601 ttgtgtttgt ttttgttgac aaatgttttg gatgcgttta caatgcaatc agttagaaag
    51661 agcgaaaact gattatagat ttgaggagag atgtacatgc ttaaattacc agaagatttt
    51721 atttttggcg gagccacagc agcctatcag gttgaaggag ccactaagga aggtggcaaa
    51781 ggcgctgttg cttgggatga ctttttagag gaacaagggc gattcagtcc cgatccggca
    51841 agtgattttt atcatcaata tgcaaaggac attgaattgt gtgaacgttt tggtgtaaat
    51901 ggtctaagat tatccattgc ttggagccgc atttttcctg atggagctgg gaaaccaaat
    51961 ccagaaggaa tcgcttttta tcatcgtgtg ttcgaagaat gtaagaaaag aaacgtaacg
    52021 ccgtttgtga cgttgcatca ttttgatacc cctaaacgtt tgtttgacca tggagatttt
    52081 ttgaatcgag aaacaataga agcatttgtt tcctatgcca ttttttgttt tcatgaattc
    52141 aaagaagtca aggtgtggag tacctttaat gaaatctatc cagttgcaac caatcaatat
    52201 ttgttaggtg ttttcccacc aggaatcaaa tatgatttta caaagatcgt cgcctgtctt
    52261 cataatatga tggttgctca tgcccgtgta gtgaactatt tcaaagaaaa cgaacttccg
    52321 ggtgaaattg gtgtagttca ctcattagag acgaaatatg cggcaacaga cgcgccggaa
    52381 gataagcatg ctgccttttt agacgatgca ttgtcgatcc gtttcttatt agatgctacc
    52441 tatttaggct attattcaac tgaaaccttg actgctcttg atgagatatg tgaagcaaat
    52501 caggccagct atcattttcc tgaagaagat ttcgttgaat tgaaaaaagc atccacgcgt
    52561 aatgactact tagggatcaa tcactaccaa tgccattttg tcaaagctta cgatggagaa
    52621 aatgcaatcc atcataacgg tacgggtgaa aaagggacct ctgtttataa agtgaaggga
    52681 atcggagaac gtatttataa agaaggaatt cctcgaactg attgggactg gctgatttat
    52741 ccagaaggtc tatatgactt gttgctgcga attaaaagcg attacccaca ttacaacaaa
    52801 atctatatca ctgagaacgg gatggggtat aaagatcagt ttgaagatgg cattattatg
    52861 gatcagccga gaatcgacta tttgagagtc tatttggaat ccttaagcaa ggcgataaca
    52921 gccggggtaa atgtgaaggg gtattttttg tggtcgctta tggatctttt ctcatggaca
    52981 aatgggtata acaaaaggta tggcttgttt tatgttgatt ttgaaaccca gaagcgttac
    53041 ccaaaagaat cagcttactg gtacaagttg gtcagtgaaa ccaaaacgat tatttaataa
    53101 gtgataatgg aggaagaaaa gatgaataaa gaggaattac aaatgcttgg ttttgagatc
    53161 gttgcatatt caggcgacgc tcgcagtaca ttattgaaat tattgaaaga agtaagacaa
    53221 ggaaactttg aacatgttga ttcggcgttg aaagaagccg acgaaaattt aacccttgca
    53281 cataattcgc aaacaaaaat tttagcggaa gaagcatctg ggaaagagat ggagatggga
    53341 ttcattttta tccatgggca ggatcattta atgaccactt tattgctgag agatctgatt
    53401 caagacttta ttgtactgta cagacagaat caaggatagg agaagaacgc tatggatgga
    53461 atcattaaac aaatcgaaaa agggaaacca ttttttgaaa aaatctcacg taatagttat
    53521 ttaggagccg ttcgtgacgg atttttaaca gcgatgccag ctattttatt ttcaagcatt
    53581 ttcattttga ttgctgcggt tcctgagatt tttggttacc aatggcctga atctgtctcc
    53641 acatggctat ggaaagtcta taattattcg atgggtgttg tagggttact cgtttcagca
    53701 accactgctc gctgcttggc tgattcgatg aatcgtcgct taccgaaaaa caaagtaatc
    53761 aacaccacct ctgtgatgct ggcctctgtg gtaagtttcc tgttgttaag cgtctctccg
    53821 attgaaggtg gattttcaac ggaatatatg gggacaaagg gactactggc ttcctttgtc
    53881 gctgcatttt taacagttaa tttatataag ttttgtgtgc tgaaagatat taccattaaa
    53941 atgcctaaag aagttcctgg aacgatttcg caaaccttcc gagatatttt tcctttctct
    54001 ttttcagtat ttgcggccgt tttaattgat ttaggcattc gacaaatctt ccaaatctca
    54061 tttgcagaag caatcattac ggtgctgcag ccgctattta cagcagctga cgggtactta
    54121 ggaatcgcta ttatctgggg agcgatggcg ctgttttggt ttgtcggtgt tcatggaccg
    54181 tcgatcgtgg agccggcaat tgcggcaatt atctatgcca atgtggaaac caatctgcaa
    54241 ttgttcaatt ccggtgaaca agcttctaaa gtactgacgg tgggtttagg gaattttgta
    54301 ggaactatgg gaggaactgg ggcaacattg gttgttcctt ttatcttcct actctttgct
    54361 aagtctaagc agttgaaggc agttgggaaa gcttccttta ttccagtaag tttcgctgtc
    54421 aacgaaccat tgctatttgc ggcacccatg attttgaacc cttatttttt tattccattt
    54481 ttgttaacac ctgttgtaaa cgtttgcctg ttcaagtttt ttgtcgatgt attgggcatg
    54541 aacagcttta tgtacgtttt gccttgggca actccagcac cgatcggttt ggttctagga
    54601 acgggcatgg gtatcttagc ttttgttcta gcagcggttc taatcgtcgt tgacttcctg
    54661 atctatctac cattttgcaa agcatacgat gctgtcttag tagagaaaga agcacaaaaa
    54721 gcagaggtga tcgcaaccga ggaaggtgct tctgccgatg tacgtgagga agtacctgtg
    54781 gcagcgagtc aagttatcag cgatgcaaat aaagaaatca atgtgttggt actttgtgct
    54841 ggcgcaggga caagtgcgat gttagctaat gctttaaaaa aaggtgcatc tgaatcgaaa
    54901 gcaccgatca gtgcaatggc gggtgcttat ggttctcatt acgaaatgct gcaggatttt
    54961 gatatggtgg tcttagcacc gcaagtcaat tcttactatg atgatatcaa agaagacacc
    55021 gatcgcttag gaatcaagct ggttgcgaca aaaggagcag aatatatcgg attaacgaga
    55081 gatcctgaaa aagctgttca gtttattctg aaccaatttc aataaaggag gagccataat
    55141 gaaaatggga atttggttgc gtaaagctgt tttatttgca gctgtgttag gggctttggg
    55201 ggctgtttca aaaactgttg aagcatctgc cagcgaattt attcgcggag cagatatttc
    55261 gatcctgtca gatatggaaa aatctggtgc aaaatattat gaagacgata tttcaaaaga
    55321 tgccttagag attttaaaaa ataatggtgt caactatgtt cgactgcgtt tgtggcagga
    55381 tccttataat agtaatggag aaagttatgg agcagggaca aatgatctgg aaacgacgat
    55441 tgaccttgca aaacgagcaa aaaatctggg acttaaggtg ctgctggatt ttcactacag
    55501 tgatttttgg gttgatcctg ggaaacagaa tcttcctaaa gcatggcaag gtctgacctt
    55561 tgaagaaatg aatacggcat tgtacgacta tacaaagaac gttctgtcgg aaatgaagca
    55621 gctggacgtt tatcctgata tggttcagat cggcaatgaa ctgaatagcg gaatgctctg
    55681 gccttatggc aagtcttggg gagaaggcgg gggagaattt gatcgattgg ctgctttttt
    55741 gaagtctggt atccaagctg ttcgagacac tcaacctaaa acaactccag taatgctcca
    55801 cttagctgat ggtggcgata caggagcatt tacttggtgg tttgatgaaa tcacgagtcg
    55861 aggtgtgtca tttgatttaa ttggagtgtc gtattatccg tattggcacg gttcaatgaa
    55921 tgaacttcaa accaatatga atgcgatcag ccaacggtat ggtaaagaag tgatcgtagt
    55981 tgaaacagct tatgggcata caacagcgaa tgccgatact atgccaaatg cttttggtga
    56041 agccgaagca gctgcggggg gatatgaacc aacaccgaca ggacaagcag agtatttgct
    56101 ggatcttgcc gatcggattc aggcagtacc gaataatcgc ggggcgggct tcttctattg
    56161 ggaacctctt tggtacaacg gaaatgtgag ttgggcaaca caagcaggta tgaattatct
    56221 cagtgttcaa tccactatgg gaaacgaatg ggacaaccaa gcgatgtttg attttgctgg
    56281 aaacgccttg ccaagcttgc gtgcgtttaa acaagcaggg gcgcaaataa atctagttaa
    56341 aaatgcaagt ttcgaaagcg atggaccaac gaccagtcct tccaattggc aaacctggtt
    56401 tcaacaagga accgctaagg gtacgatcaa aacggaagcg ggcacttttt ttggggacta
    56461 caaactaact ttttggcagg atcaagcata cgaggcatca gcttatcaaa atgttagtgg
    56521 cataagcaaa ggaacttaca cattgagtgc ctatgtaatg tctgggggag agtttgatac
    56581 taatcagtta tatatcaaaa attatggtgg agcggaacta aatactgcgg ttgttaaaac
    56641 aagtgaatgg acaaaaatta caatcaaaga cattccaatt acaacaggtg tttgtgaaat
    56701 tggggtttat agcaaggctt caggaaatgc ttggacgaac gttgattttg tttcattggt
    56761 gaaacaatag actgcggatc gtttcgattc aagatcggag gttcccttca gccacatcgt
    56821 tagcgtagaa ggcaggaatc gaacctgcac ctcgcctttg ccaaaaataa ccagaaacga
    56881 tttccagtca tttttaccaa aacgagtaaa gcaccagact tcatctacac tttgtattat
    56941 gctaaagaga gttcaacgat ttcgtaagaa gcaatgaaaa aattgcaaaa agttgtgcag
    57001 ataacataaa gtttgtttaa gaaagggtaa ccttagaatt ccgtcgtgaa tatgatttga
    57061 gcacttagat ggatagaaaa aagttctcgg aaaacagttc gtttttcgag aacttctttt
    57121 tatgataaga atgaaacaaa ttaacttata ttatagtctc ttttggtagg gaaccatctt
    57181 atttttaatg aaaatcaagc attttagcta ttgaagaatg ttgatttata gggattcccc
    57241 ctccatatta tgttggctct ttattcccac tccactgttg ctggtggttt agaagtgatg
    57301 tcatacacga tacggttgac gtgggctact tcgtttacga tacgtacaga gattttttgc
    57361 aagacttccc atgggatacg ggcaaagtca gcggtcattc cgtcgattga agttacagca
    57421 cggataccga ctgtgtagtc gtaagtacgt ccgtcaccca taacaccgac agagcggatc
    57481 cctggaagaa cagtgaaata ttgccagata tcacggtcaa gtccagcagc tgcgatttct
    57541 tcacgcaaga tagcatcaga ttcgcgtacg atttctagct tgtcttcagt gatttcgcca
    57601 agaacacgaa taccaagacc aggaccaggg aatggctgac gccaaacgat tgaatcaggc
    57661 atgcctaact gagtccctaa ttcacggact tcgtctttga ataatgtgtt taatggttcg
    57721 atcaattcaa attgcatatc ttctggcagt ccacctacat tatggtgaga tttgatcgtc
    57781 tgtgctgttt ctgtaccaga ttcgataacg tcagtataca acgttccttg tgctaggaaa
    57841 gagacgcctt cttcaccagc aagttttgtt gcttcgtcat caaaaacata tacgaattca
    57901 ttcccaatga ttttacgttt ttgttctggg tcagatacac ctttcaactt gtctaagaag
    57961 cgatctcttg cattgacttt gatgatgttc aagccgaatt ttccgcctag actttccatt
    58021 acttgttccg cttcgccttt gcgtagcaga ccgtggtcaa caaagataga tgtcaattgg
    58081 tcaccgatcg ctttttgtaa aagaacccca acgacactag aatcaacgcc acctgaaaga
    58141 ccaagcaaga cttttttgtc accaacttgt tcacggattt tttctacttg catatcgata
    58201 aagttatcca ttgtccagtt accttcacag tgacaaacat caaaggcaaa gtggcgcagc
    58261 aattcgattc cgtattcgga atgacgtact tctgcatgga attggatacc ataaaaatta
    58321 cgtgttgtgt cttggataga agcaatcgga caatctttgc ttgttccgac tgtttcaaag
    58381 ccttcaggaa ctttagttac caagtcacca tggctcatcc atactgtttg ttgttttggt
    58441 gtttctttaa acaagacagc ttgatcgctt gttacttcta acaaggcttt cccatattcg
    58501 cggtttttag caggctctac ggaaccgcca agtttgtacg tgatcaattg cataccatag
    58561 caaatcccta atacaggaat acctaaattg aagatctcag gatcgatccc aaatgcattc
    58621 tcatcatata cactgtttgg tccgccagaa agaatgattc cctttggatt catcgctttt
    58681 acttcttctg cagtaatgcg gtggctcaat aattcggaaa agactccaag atcacgaata
    58741 cgtcttgtga tcaattggtt atactgacta ccgtagtcta atacgataat cttttcaacg
    58801 ttcgtcatat cggcaacgtt tgtcaaattt attaccccta tccctttaaa ttttatctca
    58861 aaatagtcaa actattttaa aatgctaata ttttcgcatg aaaatcgaat cgatgatatg
    58921 attttcagtt ttatggagaa ttatatcggc tcttcctcgt gtaggcaaga tatattcctg
    58981 taaatttttc aaattcacat ttttccatac atcttttgcc attttaaagg cttcttcacg
    59041 atctccgata gcaaaacgat aataataatt ctctggattt tggaaagccg tatccagtaa
    59101 cgcaccaaaa cgatccaaat accatttttc gatcagcacg ggatcggcat cgacatagac
    59161 agaaaaatca aaaaagtcgc tgacgtagat ttgctgattc gcaggtaatt gaagcgtatt
    59221 gatcccctca acgatcaaga tatcaggctg ctggatcaat tcgtacttgt ctggaatgat
    59281 atcatacaca tcatgtgagt aaacaggtac ttttatctct tcctgaccag attttacacg
    59341 gttcaagaaa tcgatcagca tctccatatc ataactttca ggaaatcctt tacggtccat
    59401 gattccacgc tcttttaata tacgatttgg ataaagaaat ccgtcagtcg tgatcagctg
    59461 aacgtttctt cgtttgaagg tacgagacaa aatcgtctgc agcagtctgg ctgttgtact
    59521 ttttccaacg gctacacttc cggcgatccc gataataaaa ggcggtactt ctacgtattt
    59581 atgaagaaat aaacctttac tcaagaacaa agattcaaat tctttcatat aaagataaat
    59641 cagatgagca agaggtacat agatatctcg aacatcctgc atggaaatgc ggtcattcaa
    59701 acttttgata ttatctagtt catcttgtgt caaaggtgct tgcccattac gataaaattc
    59761 tcgccattct tctcttgcca cacggtagta gttcattgga tcattcatat tagcctcctg
    59821 cgttctccca tcaatggaac tattttaaca caaaataaac gcaaacccca ccacttaaga
    59881 gagattgccg atctttttgt ataatcttta taatagaaag aaacggagtc ttattcttga
    59941 cttgcgcacg gatacataaa aggatagact gttgtcatga aagaaatgag aaggatggaa
    60001 gacatgacaa atcattacta ttctgaacgc ccagatacgg cgcacgattt tgaacaatgg
    60061 gcttttgagt taaaaggcag aacctttcaa tttgtaacag attctggtgt attttcacgt
    60121 gaaacagtgg attatggttc acgtgtcctg attgatgctt tcgattggga agagttacct
    60181 gatgaaggaa agatcttaga tgtgggatgc gggtatggac cgattgggtt agctttggca
    60241 tttgcgacac agcggtttgt tgaaatggtc gacattaatg aacgagcggt aagtctagcg
    60301 caaggaaacg caaagcgtaa ccaaatcgaa catgttgata tccatcaatc gaatatctat
    60361 gaagcagttc atgaagaaac ctatgcagct atcgtaagca atccgccgat ccgtgctgga
    60421 aaaaaagttg tacatgaaat tttgacaggc gcatatcctc ggttgaagaa gggcggtaca
    60481 ctgacaattg tgatccagaa gaaacaagga gcgccgagtg cgcaaaagaa aatggaagaa
    60541 acatttggta atgcagaaat cgtcacaaaa gataaaggat attatatcat caaaagtgtg
    60601 aaagaataac tagctgaaaa atagtggagc gacgaaagag tggaactggg cgtgcatgta
    60661 cgggtacgta tcagttcttt ttttgttgag aagtttttaa aaacaaagaa ctattttttc
    60721 ttcaaaaaga aatagtagga acagagtcgt tgttttttac gataaaataa ataagataca
    60781 gaacttgtat agaaggctgc caagtcggta gataaaggag gtttttatca aaaaacatac
    60841 tttccttttt tctctattct tttcttgggg gttttggtac actgataggg aatttagata
    60901 gaagggagcc aataggatga agctgaccat cagagatatc gcagaaatgg caggcgtatc
    60961 agtaacaact gtctcacaga ttttgaataa caaaggaagc agatttagtg ataagacaag
    61021 aaaaaaagtg ttgtcgatcg tgaatgaaca tcattataag cccgattatt tcgcttcaaa
    61081 tgtgatcaat cgacattcaa agaccatcgg aatgatcgtc cctgatgtca ctgatttctt
    61141 cttctccaaa ctgatcgaag gtgtagagag ctatttgaat cctttgggct atgtgatcat
    61201 gttatgcaat tctcgtcata gccaagaaaa tgagatcaag tatttacaag agctttccca
    61261 ccgctcagtg gacggaatct tgctggccac accaaatatt ttacctgaga aatatgcatt
    61321 agacagcggt ttttatcaaa aaatgccaat cattttaatc gatcgcggat tgaatcgtcg
    61381 ggataacgga cggcttatcg taaaggaata tgagggcgcc tatcaagctg tttcgctgtt
    61441 tattgaaaat ggccatacga agatcggcat gctgaaggaa accactggat actatcaatt
    61501 agaagaacgt ttcaacggat atcgtcatgc gctgaaagat catggcctgc cttttaatgg
    61561 gaaatttgtt gaacatggag agttgactgt ccaaggtgga tatactgcat caaaaaaatt
    61621 actcaaacat aaagatatca cggctatttt ttgcgggaat gatgccatgg cgatcggatg
    61681 ctatcaggcg atcgatgaat tgggcaaaaa aatcccagaa gatatctcag tcataggatt
    61741 cgatggatta aagctgtcag aatacatgat tccgcaattg acgacagtgc agcagccaag
    61801 ttttgatatt ggattttatg ctgcacgttt cctgatcgat acgatcgaat ttcctcaaag
    61861 aaaagttccg aacaaagtgt tcgaaacaaa attaattatt cgtgagagcg taaaagtatt
    61921 gacaaagggc taactcggcg gtatattgct tatgtaagca tttacaaagg ctagttagtg
    61981 ctttttttat ttttagtaaa ccagtttact aaagtggttt acctttccta aaaataatgt
    62041 ataataccga atgaagacaa ggaggatcag gcaatgcgaa tggtggatct gattgaaaaa
    62101 aagagggatg gaaacgagct gtcgaaagag gaaatcgagt acatcgtcac gaactatacg
    62161 aatggaaaga ttccagatta tcaagtcagt gcattattga tggcaatttt ttatcaagac
    62221 atgacaaatg aagaaatcac gaacttaaca ttggcgattg ctaattctgg tgatgtcatc
    62281 gatttgagtt ctcttgaagg aataaaggta gataagcatt cgacaggtgg cgttggtgac
    62341 acgaccacat tgattttagc ccccctagta gctagtgtcg gagtgacagt ggctaaaatg
    62401 tctggacgcg gtctaggtta cacaggtggt acactagaca agctagaagc aattcccgga
    62461 tttcagatcg agctttcgga tgaagaattt gtacgcattg ttaatgaaag taaagtagcg
    62521 gtaattggtc aatcaggaaa cttggcaccg gcggacaaaa aattgtatgc cttgcgtgac
    62581 gtcacagcga ccgttgattc tcttccgctg attgccagtt cgattatgag taaaaaaatc
    62641 gcggcaggag cagatgcaat cgttttggat gtgaccactg gagacggtgc tttcatgaag
    62701 aacatcgaag atgcaagacg tctggcaaag accatgacga gcattgggaa actggctaac
    62761 cgagaaacag tggcggttat ctcagatatg tcagaacctc ttggcgaagc tatcggaaat
    62821 agtctagaag ttgtcgaggc aatcgaaaca ttacaaggaa atggtccaga agatctagta
    62881 gaaatgtgct acgccttagg aagccaaatg gttgttcttg ctggcaaagc taagacaacc
    62941 gatgaagcaa gaacgttgct tcaagaagct ctggaatcag gtaaagcgtt agcaaaattc
    63001 aaagaaatga tccaaaatca agggggagac ccagcgatcg tggaacatcc agaacgaatt
    63061 ttgactgcac gctacacaat ggagcttcct gctaaacaaa gcggagttgt gtcaaaaatc
    63121 gtggcaaatg aattaggaat tgctgcaatg atgctaggtg ctgggcgtaa aaccaaagaa
    63181 gaagatattg atcacgctgt tggattgaaa ctgcataaaa aaattggtga cactgtgaca
    63241 aaaggtgagt cactattgac gatctatagt aacgacgaag aaatcacttc tgtcattgaa
    63301 ttattatata aaaatattga aattggtgaa tcggcaatga agccaacgtt gattcatgac
    63361 atcatcacag aataaaggaa aaaagcggac tttaccgttt taaagaaaag gggaaaagaa
    63421 atggaactaa accgaatgat cgaccatact attttgaaag cagacgcatc taaagaagct
    63481 gtgatgcaaa ttatcgaaga agcaaaaaaa taccattttt attctgtctg catcaatcca
    63541 gcatgggttg cgctagcgaa agaacaatta caaggtgagc cagtagccgt atgtacagta
    63601 atcgggtttc ctctaggagc aaacacaccc gagacaaaag catttgaaac aacagatgcc
    63661 atcaataacg gtgcagatga agtcgatatg gtcatcaata tcggtgcttt gaaatcaaag
    63721 gatgatcaac aagtccaaaa agatatcgaa gcagttgttg aagcagcaaa agatcgtgcg
    63781 cttgtcaaag ttattattga aacatcgtta ttagatagag aagaaatcat ccgagcttgc
    63841 gaaatagcaa aagctgcagg cgctgatttt gtcaaaacat caactggttt ctcaacaggc
    63901 ggagcaaagg tagaggatgt ccgtttgatg cgtgaaactg taggaccaga aatgggtgtc
    63961 aaagcttccg gcggtatcca taacgaagaa gaagcaatgg caatgatcga agctggtgct
    64021 acaagaatcg gaacaagcgc aggagtagca atcgtatctg gttcaactgg tgaaggctat
    64081 taattttttc aaaggagaag ctcatggcag aactcaaaca agaatggatc aacacagcta
    64141 ttgaagcgtt agataaagcc tatgttcctt attcccattt tccggtaggc gcttgtctcg
    64201 taacggaatc aggcaagatt tatcaaggca tcaacattga gaatgcatca tttggattaa
    64261 cgaattgtgc cgagcgcaca gcttttttca aagcagtatc tgaaggagaa aaaacattta
    64321 cgcatctagt cgtagcgggg cacacccccg atcctatctc tccttgtggt gcatgtcgac
    64381 aagtaatggc agaattctgt tcccctgata tgccggttac gctagtagga gacaatggtg
    64441 tgacgaaggc aacgaccgtt cgtgcactgc tgccttatgc atttactgaa aaagatctat
    64501 aactcatgtt cattcagtca gctgactgtt gaaaaataaa acaattattt taggaggctt
    64561 tacagaaatg aaaaaagcaa aattatttgg tttaggtgct gtcgcactag cagcagggtt
    64621 atttttaggt gcatgcggca acaacggctc tactgattca tccggtggca aaagctcatc
    64681 agacacaaca actgcagcat tgatcacaga cacaggtggt gtagacgacc gttcattcaa
    64741 ccagtcagct tgggaaggac tagaaaaatg ggggaaagat caaggacttt cacgaggaaa
    64801 tgatggcttc caatatttcc aatcttcaaa tgaatcagat tacatcccaa atattgacca
    64861 agcattgaat gctggattca aaacgatttt tggtattggt tataaactaa aaccagcgat
    64921 ccaagaacaa gcaacaaaca atacaggtac gaactttgtt attatcgatg atgtcatcga
    64981 tggattagat aacgttgtat ctgcaacttt caaagacaac gaagcttctt atcttgcagg
    65041 tgttgcagca gcttatacga ctgaaacaaa cgtagtcggc tttatcggcg gtgtaaaagg
    65101 tgaagtaatc gatcgttttg atgctggatt caaagccggt gttgatgcag gtgcgaaaga
    65161 attaggaaaa gaaatcaaag tattgaatca atatgctggt gacttctctg ctccagataa
    65221 aggacgttca attgctcaag gtatgtatgc acaaaacgca gatatcatct tccatgcttc
    65281 aggcggtaca ggtaacggtg tattccaaga agcaaaatca ttgaacgaat caggcgacaa
    65341 aaaagtttgg gttatcggtg ttgaccgcga ccaatcagac gaaggcgaat acacattaaa
    65401 cggcgaaaag aaaaacttca cattgacatc tacactgaaa gcagttggaa cagtagtaga
    65461 agatttggca caaaaatcag cggacggtaa attccctggc ggcgaacaca ctgtttatgg
    65521 attgaaagaa gacggtgttg gtctaacaga aggacaactt tctgacgaag cgaaaaaagc
    65581 agttgatgaa gcaaaagaaa aaatcatttc aggtgacgta aaagttccag aaactcctga
    65641 agaaaactaa aaccatttct atagccttgt tagacacatg tttgacaagg ctatggtttg
    65701 attagaacaa gaattgttca tttatccaag ctgccttgtg acagtttgga taagggaaca
    65761 actccctgaa gtaatgtttg tttcaaaaga agaacgctca ctaagcgtga atcttagtca
    65821 atcgttgccc tagatagaaa tcgttaggat gtgaaaaaaa ggtggttcaa gaaaatattg
    65881 tcatagaaat gcagaatatt accaaagaat ttggaacatt caaggcaaat gacaacatca
    65941 atttacaagt aaaggcagga gaaattcatg cgttgcttgg agaaaacggt gctggcaaat
    66001 ctacattgat gaacgtgctt tccggattat tagagccgac atcagggaaa attttgatgc
    66061 gtgggaaaga agtaaagatc acaagtccga ccaaagccaa ccaattaggg attgggatgg
    66121 tccatcagca ctttatgctt gttgatgcct ttactgtaac agaaaacatc gtgttgggaa
    66181 gcgagcctag tcgcgcaggg atgcttgacc ataaaaaagc acgaaaagag atccaaaaag
    66241 tttctgaaca gtatggattg tcagtcaacc cagatgctta cgttcgtgat atttcagttg
    66301 gaatggaaca acgggtagaa attttaaaaa cactttaccg aggagcagat gtactgattt
    66361 ttgatgagcc gacagctgta ttgacccctc aagaaattga tgaattaatc gtgatcatga
    66421 aggaattagt caaagaaggc aagtcgatca ttttgattac gcataagtta gatgaaatca
    66481 aagcagtagc tgaccgctgt acagttatcc gccgtggaaa aggaatcggt acagtcaacg
    66541 ttaaagacgt aacctcacaa caattagccg atatgatggt tggaagaacg gtttcattca
    66601 aaacgatgaa aaaagaagcg aagcctcaag aagtcgtttt gtctattgaa aatctagtgg
    66661 tgaaagaaaa tcgtggatta gaagccgtga aaaacttgaa cttagaggtt cgtgctggcg
    66721 aagtacttgg tatcgctgga atcgatggaa acgggcagtc ggagttgatc caagctttga
    66781 ctggtttgcg aaaggcagaa agcggacata tcaagctaaa aggggaagac atcactaata
    66841 aaaaaccccg aaaaatcact gaacatggtg taggacacgt gccagaagac cgtcataaat
    66901 acgggttagt cctagatatg acattatctg aaaatatcgc actacaaacc tatcatcaaa
    66961 aaccttacag taaaaacggc atgctgaatt attcagtaat gaatgaacat gccagagaat
    67021 tgatcgaaga atatgatgtt cgaacaacga atgaacttgt tcccgcaaaa gctttatcag
    67081 gcggaaatca gcaaaaagca attatcgctc gtgaagtcga ccgagatcct gatctgttga
    67141 ttgttgcaaa tccaactcgt ggactggatg taggtgcgat cgaatttatt cataaacgtc
    67201 tgatcgaaca aagggacaaa tacaaagcag tgttactgat cagtttcgaa ttagaagaaa
    67261 ttttaaatgt ttcggatcgt attgctgtta tccatgaagg agaaattgtc ggtatcgttg
    67321 atccgaaaga aacatctgaa aatgaattag gtctattgat ggcgggctat accttagaag
    67381 aagcacgaaa agagttagaa aagggggctg gcgcacatga atgatcgcag cgaaaaattc
    67441 cgtaatttat tagtgcctgt actttcagtt gttttaggat tgatcttagg cgcgatattg
    67501 atggctgctt ttggttttca gcccatccaa ggttattctt ctatgctaga tgcagccctt
    67561 ggttcgcaaa gagggatagg tgaaacgtta aggcaagcaa cgccgttgat ttttacagca
    67621 ttaggttttt ctgtagctaa ctcagctggg tttttcaata ttgggctttc tggacaagca
    67681 ctttgcgggt ggatcgtcag tgtctgggtg gcacttgcgt ttccagatct gccaaaagtt
    67741 gttttgcttc cgttatgcgt gatcttaggt gccttagctg gtgcgatggc agcggctgtt
    67801 ccaggtttac ttcgagcatt ttttggaaca agtgaagtga tcgtaacaat catgatgaac
    67861 tatatcttat tatacgtaag cacatttgct ttacatgaag tgatgcctga aagctatcgc
    67921 tcaagtttgg actcttccaa tatgatcagt caaaatgcct cactgcggtt accatggtta
    67981 acacaagctt ttggcggctc tcgcgtaaac tcaggattat ttttagcaat catcgcgctt
    68041 attttcgttt ggctgctgat gaagaaaacg actttaggat ttgaaatccg ttctgttgga
    68101 ttgaatcctt ttgcttctga gtatgcagga atgagtagta aacgtacgat cgttttgtcg
    68161 atggtcattt ctggcgcttt ggcagggctt ggcggtgtgg ttgaaggatt aggaacgtat
    68221 caaaacttct ttgttcaaac gacttcgcta tctattggat ttgacggaat ggctgtttcc
    68281 ttgcttggtt ctggatcagc aatcggtatt cttttatcag cgatcttgtt cagtattttg
    68341 aagatcggtg gacttggcat gcagacagga gcaggtgttc cgtttgagat cgtcaatgta
    68401 tcgattgctt tgattatctt ctttgtcggg atcaacttcc tgattcgttt cgtcatggct
    68461 aaagtcttcc aagggaaaaa acaagaagag atcgttgcga caatagaatc gaaaccaacg
    68521 aatcaaaacc atgaaggggg cgaactataa tgtcgactat cgaattaatt gccttgatcc
    68581 ttacttctac actggtttat tcaacaccgc taattctaac gtcactaggc ggaacttttt
    68641 ctgaacgaag cgggatcgta aacgtagggc tagagggaat catggtcatg ggtgctttta
    68701 gttccatcgt tttcaacttg acgatgagtc catcacttgg ttctatgaca ccatggatcg
    68761 ctattttagt gggtggcgtc gtaggtatcc tattttctct gattcacgca gtagcaacag
    68821 tcagtttgcg tgctgaccat attatcagcg ggacagtaat taatctgatg gcacctgctt
    68881 taggtgtttt cttgatcaaa gcatggtatg gtaaaggtca aactgataac atcacgcaga
    68941 atctggggta tttttctttc ccaggattag cagatattcc ggtcattgga ccaattttct
    69001 ttaaaaatac attcttacct gcttattgtg ctattttgat tgctgtccta gcttgggtga
    69061 tcatcttcaa gacgaaattc ggcttgcgcc tccgttcagt tggtgaaaat cctcaagctg
    69121 ccgacacatt aggtatcaat gtatatgcta tgcgttattc aggcgtactg atttctggct
    69181 tgttaggcgg tatgggcggt gctgtttttg ctcagtcgat ttccggtaac ttttcagctg
    69241 gtacgatcgt tggacaaggg ttcatctcca tggctgctat gatctttggt aagtggaatc
    69301 ctttaggggc aatgggtgcc tcattgttct ttggttttgc tcaaagcttg agtatcattg
    69361 gcgcacaatt accagttatt tcttcgattc cgccagtatt gctgcaaatt gctccatatt
    69421 tgctgacaat cattgtgctg gttgttttct tagggaaagc ttctggtcca aaagcaaacg
    69481 ggaagaacta tatcaaatca aaataatctt ttagcgaata aaaagggtgg gttacgggaa
    69541 gtgtttttcg tctcgccctt tttgttttaa aggcaataaa aataaaaaga aagaaggaat
    69601 aatcatgttt aaacgcgtac atctgatcgt catggattca gtaggaattg gagaagcacc
    69661 agatgccgat aagttcggtg atgttggttc tgatacatta ggacacattg cgaaagaagc
    69721 aggattgaac gttccacatt tagagcagct tggtttagga acgatccgtc ctcttgaagg
    69781 tgtggaatca gtgaaagatc atcaaggcta tgcgacgaaa ttagaagaag tttctgtagg
    69841 gaaagatacg atgacaggcc actgggaaat catgggattg aatatcaaaa ctccatttcg
    69901 tgtgtttcct aatggattcc ctgatgatct gatcaaacaa atcgaagaat tttctggccg
    69961 taaagtcgta tgtaacaaac cttatagcgg aacagctgtt attgacgatt acggtgaaca
    70021 ccaaatgaaa acaggcgatt taattgtata tacttctgcc gatcctgtcc ttcagatcgc
    70081 ggcacatgaa gaaatcattc ctttagaaga attatatcgg atttgccaat tcgttcgtga
    70141 tataacaaaa gatgatccgt atatgattgg ccgaatcatt gcacgtccgt acgtgggtga
    70201 accaggagac ttcacacgaa caagcaatcg ccatgattat gcgttgaatc cattcggaca
    70261 cacagtcctt gattctttga aagaagcagg aaaggacgtg attgctgttg gaaagatcaa
    70321 cgatattttc aacggacaag gaatcactga atctgtccgc acgaaaagca atatggatgg
    70381 cgttgaccag ctgctgaaag ttatgaaaaa cgatttttca ggaatcagct ttaccaattt
    70441 agtagatttt gatgcattat acggtcatcg tcgggatgta gtcggctatg cgcatgcgat
    70501 tgaagaattt gacttacgaa ttccagaatt gcttgaagcg atggaagaag acgacttgct
    70561 aatgattaca gccgatcacg gaaacgatcc gacgtttact ggtaccgatc atacgcgaga
    70621 atatgtaccg ttattggttt acagtaaaaa aatgaaagaa caaggggcat tgcctcaagg
    70681 atattattca gatatttcag caacgattgc tgagaacttt agtgtttctt cgactgaaaa
    70741 tggcgaaagc ttcttgaaat tattaaaata atctttttaa ataggagaaa gcaaatgacc
    70801 aagttaagcg agatgttaca agaaacgacc cagtttatcc ttgataaagg agtaaaagaa
    70861 attgattttg gtttgattct aggttcagga ttaggtgaat tagcagatga aatcgaagat
    70921 gctgtaatga tttcttatga tgagatcccg ttctttcctg tttcgacagt agttgggcat
    70981 gcaggaaagc tagtttatgg gtctctggca ggcaagaaag tgttagccat gcaagggaga
    71041 ttccactact atgaaggtca ttccatgcaa acagtgacct atccagtacg tgtgatggcc
    71101 ggactagggg cccattcagt cattgtgact aatgcatgtg gtggtgtcaa cgaatcattt
    71161 gtaccaggag atttgatgct gattaccgat catatcaatt ttactggtga aaatcctttg
    71221 atcggtccga atgaagatgc aatgggccct agatttccag atatgagtca agcttatacg
    71281 cctgactatc aagcaacagc aaaagcagca gcagaaaaac taggtttgga tcttaaagaa
    71341 ggcgtatata tgggctattc tggaccaact tatgaaacgc ctgcggagat ccgaatgtct
    71401 cggacaatag gagccgatgc agtagggatg tcgactgttc cagaagtcat cgtagcagct
    71461 cacagcggat taaaagtgtt aggaatctct tgtattacca acttagcagc aggtatgcaa
    71521 gcgaatctga gccatgaaga agttgtagaa acaactcaga gagtcaaaca atcttttaaa
    71581 gcattggtta aagaaacatt gacattattg taataggagg aacaataaaa tgagtgtgca
    71641 tattgaagcg aaagaaggcg agatcgccga taagatttta cttccgggag atcctttgcg
    71701 agctaaatat attgcggaga cttttttgga agatccagtt tgttacaatc aggtacgcgg
    71761 tatgttaggc tttaccggca aatataaagg agaacgtatt tctgttcaag gaacaggaat
    71821 gggaatgcca tcagccacca tctatgccca tgaattgatc cagtcgtatg gtgtaaagaa
    71881 attgattcga gtaggtacat gcggtgcatt gtcaaaagac gtccatgttc gtgatttagt
    71941 actagcacaa ggtgcagcga ccagttcttc gatgatcgaa aaaaatttcc aagcctttca
    72001 ttttcctcca atcagtgatt tcaatctatt actgaaagca tatgagatag ctaaagaaaa
    72061 gggctatacg gttcatgtag gaaatgttct ttcggaagat tctttctaca aagatgattt
    72121 gacagagacg ttccaattag ctgaacttgg cgttttagga gtggaaatgg aagcagcggc
    72181 tctttattac ttaggcgcga aataccatgt gcagacatta agtttgatga ccgttagcga
    72241 ccatttgatc accggtgaag aaacaacagc tgcagaacgt caatctacgt tcaatgaaat
    72301 gatcgaagta ggattggaaa cagcgatagc agaataagaa ggaaatgaaa aagctgccct
    72361 gcattaagta ataagaacat ccaagcaaaa atagctcctc atgtttttga cttggctgga
    72421 cttattattg caaatgtaag cttttgaaca ccgtttacca agaggttgtg aatctgccgt
    72481 ttaactctga gtcacaagga acaatttcaa aaaacagctt ttcatgtttg atgaatattg
    72541 tgacttgtga ccgagaagtt ggcaggagaa aaccgtttat acgaaaaaag ggactgtgac
    72601 aaatcgtgtc acagtccctt ttttatagtg ttttttatag gtagtatttt ttagcaactg
    72661 ttaagtcatc atttaattca taaacaagtg gttgacctgt tggaatttca agatccatga
    72721 tgtcttcgtc agagatacct tcgatatgtt tagctagggc gcgtaatgag ttaccatgtg
    72781 cagctactaa aacagttttg ttgtctagta atgctggagc aatttcatct tgccagaatg
    72841 gcaaagcgcg ttctaatgtc actttcaagt tttctccacc aggaatatcg cgtttgtcta
    72901 acattgcgta acgacggtca tttgctgcag aaccttcgtc agtttcttcc attaatggag
    72961 gaagagtatc gtaagaacga cgccagatgt gcacttgttc gtcgccgtat ttttcagctg
    73021 tttctttttt gttcaaacct tgtaatttac catagtgacg ttcgtttaag cgccatgatt
    73081 tgatttgtgg tacccaaagt tgatctgaat attctaagat caagttacat gttttgattg
    73141 cacgagttaa aacagaagtg taagcaacat caaattcgat tccagcttct ttgattttgc
    73201 gtccgccttc ttttgcttct tcgattcctt ctggtgctag atttacatca gcccaaccag
    73261 taaattggtt caatgcattc cattcactaa gaccgtgacg agaaaaaact aattttggca
    73321 tgtttagctc tccttttaag ataggtttat atttctgaaa ttttcagttc tgttttattg
    73381 tacaattttt tggaagagat ttccatcgaa aaattagata agattctatg agaaaacaag
    73441 tagagaatat aaaaaaacga acagtgccca cgcaactgtt cgtttttagg tattagatgc
    73501 ataattgctc gatttctttt tcaggtaaat tgatatattt ataacatgtt ccaactgaaa
    73561 caccgcattt tgaagaaata aattggatcg tttctttttt ttcataatag agacgtcgga
    73621 tttttttgat cgtttttgca tcgatttttg gtcttccgcc gatttttcct tttttacgag
    73681 cttcatctaa tccaactaat gtacgttctt tgatcaatgc gcactccatg tttgctaggt
    73741 tttccattaa ttggaaatag atttttccca tttcgcgcgt atcgattcct tcatcaagac
    73801 taatcaagtg cagcccgcga tctttgaata attgcgtgag ttctgttaat tgccgcgtag
    73861 atttgcctaa tcgatgaagt tggcaaatga ctagtgcatc gccttgttgc atgtcagcga
    73921 tcactgactc caaatgaaca gcagtagaat cattttgttc cacttcatat gtctcatgga
    73981 aaatttcttg acaaccaaag tcagataatt gatcaacctg agttgttaaa tcaacgtcat
    74041 ttatggttgt gcgtgcataa ccaataattt tcatggtgat tcctttctag cagtgatttt
    74101 ctaactggat ataacagtaa aaatgtagca taaagtacgt caaaaagata gttttttcgc
    74161 aaattttccg ctgtcttttt ttattggatg atcaagcagt ttttctttat ttacgacgga
    74221 ttgcgtcaag aatctcaacg cctttttctg agccgacaac tactttagaa accatgttta
    74281 ggaaaagccc atgttctact accccaacaa gttcatctaa ataggtagct aagctttctg
    74341 gctgttcgat gacttctaga tgcagatcaa tgatgtaatg accaccatct gtggttaaga
    74401 cttctccgtt ttcgtctgta cgcaagacgg gttggaatct tttttcttca aaaatatgaa
    74461 gtagctgttg ggagccataa gggatcactt caactgggag tgggaacttc ccaagtttat
    74521 cgactaattt agaagaatca acgatccaaa tcgtttcttt tgagtacgta gcgactattt
    74581 tctcgaaaag caaagcagcg ccgccgcctt tgatcccttg aaaatcagca ctgatttcat
    74641 cagcaccgtc tattgtcaaa tcgactacgg ggacttcatc aatactcttt aacggtattc
    74701 ctaatttttc cgcttgttct tttgtcgcat ttgaagtagt aactcctgtg atacgtaaac
    74761 cttcttcacg catacggcga ccaatctctt ctaccatata ataggcggtt gaaccggtcc
    74821 cgagacctac gatcatcccg tcctttacga atttcgcggc ttcaatgcca gctaattgtt
    74881 ttaaattcat ctttgcgtcc accttttttt agtttgagca tcgtgctttc ctcactattt
    74941 tagcgtgatt tacggtaaaa agataccatt cttttttatt ccaataaagc tttttcaaaa
    75001 aaaattgcat ctcttgttga catgtttttt cattcgtgat attctaacaa aggtgcttta
    75061 tctacaggtt tctgtgaaga aacgtgctga ctgtttatca aagcatattt gataaggatg
    75121 cagacattgc atttattttt tatcacaaaa aaagaaccac ctggatgtgt ggaactagac
    75181 gcaaatcgtg ggaaggagga aacaaggaat gcctacaatt aaccaattag tacgtaaacc
    75241 tcgtaaatca aaggtggaaa aatctaattc acctgctttg aacaaaggat ataacagctt
    75301 taagaaaact caaactaacg taaactcacc tcaaaaacgt ggggtagcaa ctcgtgtggg
    75361 tacaatgaca cctaaaaaac cgaactcagc tttacgtaaa tatgcacgtg ttcgtttgtc
    75421 aaacttaatt gaagttactg cttatatccc agggattggt cataacttac aagaacacag
    75481 tgttgtatta ttacgcggtg gtcgtgtaaa agacttacca ggggtacgtt accatatcgt
    75541 tcgcggtgct ttagatactg ctggtgttaa cgaccgtaaa caaagccgtt ctaaatacgg
    75601 aactaaaaaa cctaaagctt aattttaatt attaaacaaa tgaaacttgc ttctactata
    75661 tagaagtaat tactatgaac aatatttcgg aaggaggagt tacggatgcc acgtaaaggt
    75721 cctgttgcaa aacgtgatgt tttaccagat cctatttata actcaaaatt agtaactcgc
    75781 ttgatcaacc gtgtaatggt tgacggaaaa cgcgggattg cagctaacat tatctataat
    75841 gcctttgact tgatcaaaga atctacaggt aacgatccat tagaagtgtt tgaacaagca
    75901 atgaaaaacg ttcaacctgt tcttgaagtt aaagcacgtc gtgttggggg ttcaaactac
    75961 caagtaccag ttgaagttcg ccctgaacgc cgcgttactc taggtcttcg ttgggtagta
    76021 aactacgctc gcttgcgcgg tgaacacaca atggaagaac gcttagcaaa agaaatcatg
    76081 gatgctgcaa acaacacagg tgcttctgtt aaaaaacgtg aagacactca taaaatggct
    76141 gatgccaacc gtgcgtttgc tcactatcgc tggtaagatt ctctctgttt ggtcggcttt
    76201 tataagtcga cctcagatag tgtccttatt tttagcaact agatagtaaa gagaggagaa
    76261 acaaggagaa tgaccagaga attttcttta caaaacactc gtaatatcgg tatcatggcc
    76321 cacatcgatg ctggtaaaac gactgctact gaacgtatct tgtactatac tggtaaaatc
    76381 cataaaattg gtgaaactca tgaaggagct tcacaaatgg actggatgga acaagaacaa
    76441 gaacgtggta ttacgatcac ttctgctgct actacagcac aatggaaggg tcaccgaatc
    76501 aacatcatcg acacaccagg acacgtagac ttcacagttg aagttgaacg ttcattgcgt
    76561 gtacttgatg gtgctgtaac tgttcttgat gcacaatcag gtgttgaacc tcaaactgaa
    76621 acagtttggc gtcaagcaac aacttatggt gtacctcgta tcgtatttgc aaacaaaatg
    76681 gacaagatcg gtgctgactt cttatattca gtatcaactt tacatgaccg tttgcaagca
    76741 aacgctcacc caatccaatt accaattggt gcggaagacg acttcacagg aattatcgac
    76801 cttgtaacaa tgaaggctga aatgtataca aacgatcttg gaacagagat cgaagaaaca
    76861 gaaattcctg aagaataccg cgaattagct gaagaatggc gcgaaaaatt ggttgaagct
    76921 gtagctgaaa ctgatgaaga acttactttg aaatacttag aaggtgaaga aatcactgaa
    76981 gctgagttaa aagaaggtat ccgccgcgca acagttaacg tagaattcta cccagtacta
    77041 tgtggttcag catttaagaa caaaggggtt caattactat tggacgctgt tcttgattac
    77101 ttgccatcac cgttagatat cccagctatc aaagggattg accctaaaac tgatgaagaa
    77161 gtagaacgtc ctgcagacga cagcgctcct ttctcagctt tagcatttaa agttatgact
    77221 gacccattcg taggtcgttt aactttcttc cgtgtgtact ctggtgtttt acaatcaggt
    77281 tcatacgtac aaaacgcaac aaaaggcaaa cgtgaacgtg ttggtcgtat cctacagatg
    77341 catgccaact ctcgttcaga aatctctgaa gtttacgctg gagatatcgc tgctgctgtt
    77401 ggtttgaaag acacaactac tggggacaca ctatgtgatg aaaagagtct agttattctt
    77461 gaatccatgg aattcccaga accagttatc caagtcgcta ttgaacctaa atcaaaagct
    77521 gaccaagata aaatgggtgt ggctttacaa aaactatctg aagaagatcc tactttccgt
    77581 gctgaaacaa acgtggaaac aggcgaaaca atcatcgctg gtatgggtga acttcactta
    77641 gatatcatcg ttgaccgtat gcgtcgtgaa ttcaaagttg aagctaacgt aggtgcgcct
    77701 caagtatctt accgtgaaac tttccgtgct ggcactcaag ccgaaggtaa gtttgtacgt
    77761 cagtctggtg gtaaaggtca atacggtcac gtatggattg aatttacacc aaatgaagaa
    77821 ggtgctggtt tcgaatttga aaatgcgatc gtcggtgggg ttgttcctcg tgaatacatt
    77881 ccagctgttg aaactggttt gaaagatgct atggaaaatg gtgtcttagc tggctatcca
    77941 ttagtagata tcaaagctaa actttacgat ggttcatacc atgatgtcga ttcaaatgaa
    78001 actgccttcc gtgttgctgc ttctatggca ttacgtgcag cagctaagaa agctaatcca
    78061 gtaatccttg aaccaatcat ggctgtagaa gttgttatcc ctgaagatta tttaggtgat
    78121 gttatgggtc acgtaactgc tcgtcgtgga cgtgttgaag gtatggaagc tcgcgctggc
    78181 ggccaacaag ttgttcgtgc gatggttcca cttgctgaaa tgtttggtta tgctacaact
    78241 cttcgttctg ctactcaagg tcgtggtaca tttacaatga catttgacca ctacgaagat
    78301 gtaccaaaat cagtacaaga agaaatcatt aagaaaaacg gcggaaaagc tgaataattt
    78361 cttgaaaccg gatagtcctg caaagagatt atccaaagag actatatttt taatatagtt
    78421 tcgtgtaaaa taattatcga tgatatgggc atgtggtgta ccttgtgtac atcatatacc
    78481 tatcagacaa ataaaaaata tattgtattt ttaggaggac attttaaaat ggcaaaagaa
    78541 aaatttgacc gttctaaacc acacgttaac attggtacta tcggacacgt tgaccatggt
    78601 aaaactacat taacagctgc aatcacaact gtactatcta agaaaaacgg cggccaagct
    78661 atggcttacg atcaaatcga tggtgctcca gaagaacgcg aacgcggaat cacaatctca
    78721 acagctcacg ttgagtatga aacagataca cgccactacg ctcacgttga ctgcccagga
    78781 cacgcggatt acgttaaaaa catgatcact ggtgctgctc aaatggacgg agctatcttg
    78841 gtagtttctg ctgctgacgg cccaatgcct caaactcgtg aacacatcct attgtctcgt
    78901 caagttggtg ttccttacat cgttgtattc ttgaacaaag tagacatggt tgatgacgaa
    78961 gaattactag aattagttga aatggaagtt cgtgacctat taacagaata cgaattccct
    79021 ggtgacgatg ttcctgtagt tgctggatca gctttgaaag ctctagaagg cgacgcttca
    79081 tacgaagaaa aaattcttga attaatggct gcagttgacg aatacatccc aactccagaa
    79141 cgtgacaacg acaaaccatt catgatgcca gttgaagacg tgttctcaat tactggacgt
    79201 ggtactgttg ctacaggtcg tgttgaacgt ggacaagttc gcgttggtga cgaagttgaa
    79261 gttgttggta ttgctgaaga aacttcaaaa acaacagtta ctggtgttga aatgttccgt
    79321 aaattgttag actacgctga agctggagac aacattggtg ctttactacg tggtgttgca
    79381 cgtgaagaca tccaacgtgg acaagtttta gctaaaccag gtacaatcac acctcataca
    79441 aaattctctg cagaagtata cgtgttgaca aaagaagaag gtggacgtca tactccattc
    79501 ttcactaact accgtccaca attctacttc cgtacaactg acgtaacagg tgttgttgaa
    79561 ttaccagaag gaactgaaat ggttatgcct ggcgacaacg taacaatgga agttgaatta
    79621 atccacccaa tcgctatcga aaacggtact aaattctcaa tccgtgaagg cggacgtaca
    79681 gttggtgccg gtgtcgttac agaaatcaaa gcttaattca cttaagtttt aaagtaaagc
    79741 aacatttatt cggacgtaat cgctcggaac gaaggtctta ggatctttgt tccgagcttt
    79801 tttatttatt gcaggatcag gaaaaaagac aggagtatta taaatagaag aaacacgaaa
    79861 taaaagagtc agtattttct aaatgaaact ttatgaaaac aaaatgtttt ttacaagaaa
    79921 aagccttgct ttgtgagttt atatatatta taatgacaaa ggtgcgttta ctttagtgaa
    79981 ttgcacagac gtaatcagaa tgatttcgtc ggctaagaaa cgagaggttg cgacacgccc
    80041 ggacgctttg ccacgaacga gcgtgacgga aattttcgtg gagctatgtc tacttttcaa
    80101 aataggcgaa ggagggaaca agatggcaaa acaaaaaatt cgtatccgtt taaaagcgta
    80161 tgaacaccgt attttagatc aatcagcgga taaaatcgtg gaaactgcaa aaagaactgg
    80221 agctagcgtg tctggtccaa ttccattacc aactgaacgc agtctttaca caatcatccg
    80281 tgcgactcat aaatacaaag attcacgcga acaattcgaa atgcgtacac acaaacgtct
    80341 tatcgacatt gtgaacccaa caccaaaaac tgttgatgct ttgatgaagc tagacttacc
    80401 atctggtgta aacatcgaaa tcaaattata aaactaaaac aaaaataatg gaggtgtact
    80461 catgaccaaa ggaatcttag ggaaaaaagt gggaatgaca caaatcttca ctgaatctgg
    80521 tgaattaatt ccagtaactg ttgtagaagc tactccaaac gtagtactac aagtgaaaac
    80581 aatggaaacg gatggctacg aagctattca agttggttac caagatatgc gtgaagtttt
    80641 atcaaacaaa cctgcgaaag gtcatgttgc aaaagcaaac acggctccta agcgcttcat
    80701 tcgtgaattc aaaaatgttg agctaggaga atatgaagta ggaaaagaaa ttacagtaga
    80761 tgttttccaa gcaggagaca ttattgatgt aacaggtact acgaaaggta aaggattcca
    80821 aggggttatc aaacgccacg gacaaagccg cggaccaatg gctcacggtt ctcgttacca
    80881 tcgtcgtcct gggtcaatgg gtccagttgc acctaaccgt gtatttaaaa acaaacgtct
    80941 tgctggccgt atgggtggca accgcgtaac tatccaaaac ttggaagttg tacgtgtcga
    81001 tgcagaaaga aatgttatct tgatcaaagg taatgttcct ggagctaaaa aatctttaat
    81061 tactatcaaa tcagctgtga aagctaaata attgtaggaa gaaaggagga actaaggaat
    81121 gccgaatgta gcattattca aacaagatgg aagccaaaac ggtgaaatca cacttaacga
    81181 agaaatcttc ggaattgaac ctaatgaaag tgttgtttac gatgcaatcg taatgcaacg
    81241 tgcgtcatta agacaaggaa cacacgcggt taaaaaccgt agcgctgttc gcggcggcgg
    81301 acgtaaacca tggcgtcaaa aaggaacagg tcgtgcacgt caaggatcta tccgctcacc
    81361 acaatggcgt ggaggtggcg tagtctttgg accaacacca cgttcttaca gctacaaact
    81421 tcctaaaaaa gttcgtcgtt tagcaatgaa atctgtattg tcagaaaaag ttgctgaaaa
    81481 caacttggta gcaatcgaag gcttgaactt cgacgcacca aaaacaaaag aattcaaaca
    81541 agttcttgct aacttatcta tcgactctaa agtattggtt gttctagaaa aaggtaacga
    81601 ctttgctgct ttatcagcac gtaacttacc aaacgtttct gtagtaactt ctgataatgt
    81661 gagtgtgcta gacgttgtat caaatacaaa agtgttagca acacaaactg ctcttactca
    81721 aattgaggag gtgcttgcat aatgaactta ctagacgtaa tcaaacgccc agtgatcact
    81781 gaaaaatcaa tgcttgccat ggatgacaag aaatacactt ttgaagtgga cactcgcgca
    81841 aacaaaactt tagtaaaaca agcggtagaa gctgcttttg acgttaaagt caaaaacgtg
    81901 aacatcgtga acgtacgtgc gaaattcaaa cgtatgggca aatatgcagg atatacaaaa
    81961 aaacgtcgca aagcgatcgt tacattgact gaagattcaa aagaaatcca attattcgaa
    82021 gctgctgaat aagcactaat agataactca ttaaattagg agggaaaaac gtggcgatta
    82081 aaaagtacaa acctacctca aatggtcgtc gtaacatgac aggttctgat tttgctgaaa
    82141 tcacaacatc aacacctgaa aaatcattgt tacaaccatt gaaaaaccgt gctggacgta
    82201 acaacaacgg ccgcatcact gtacgtcatc aaggtggcgg acacaaacgc caatatcgcg
    82261 tgatcgactt caaacgtaac aaagataacg ttgttgcaac tgttcaaacg atcgaatatg
    82321 atccaaaccg ttcagctaac atcgcgttag tacactatga agatggagtg aaagcttaca
    82381 tcttagcacc taaaggacta gaagtgggta tgaaacttgt ttcaggtcca gaagcagata
    82441 tcaaagtcgg aaatgctctt ccattagaaa acattccagt tggtactgtt atccacaaca
    82501 ttgaaatgaa acctggtaaa ggtggacaat tgatccgttc tgctggtaca agtgctcaag
    82561 tacttggtaa agaaggcaaa tacgtattga tccgcttgaa ctctggtgaa gttcgtatga
    82621 tcttagctgc atgccgtgct acaatcggtt ctgtaggtaa cgaacaacac gaattaatca
    82681 acatcggtaa agctggacgc tctcgttgga tgcgtaaacg cccaactgta cgtggtagcg
    82741 taatgaaccc taatgatcac ccacacggtg gtggtgaagg taaagctcct atcggacgta
    82801 aagcaccagt ttctccttgg ggtcaaccag ctcttggtta caaaactcgt aacaaaaaag
    82861 ctaaatcaga caaacttatt gttcgtcgtc gcaaaaataa ataattactt tgaccatgtg
    82921 tcgcacattt tgagaggagg ttcaccatgg gtcgtagttt aaagaaagga ccttttgtcg
    82981 atgagcattt aatgaaaaaa gttgaagctc aagcaggggt tgaaaagaaa aaagttatca
    83041 aaacttggtc acgccgttca acagttttcc caagttttgt tggatatacc atcgcagttt
    83101 atgatggacg taaacatgta ccagtttaca tccaagaaga catggtagga cacaaattag
    83161 gtgaatttgc acctacaaga acttatcgtg gacatgctgc tgacgataag aaaacgaaac
    83221 gctaatttga ggggaggata aatccaaatg gcagaacaaa tcacatcagc taaagcaact
    83281 gcaaaaacag ttcgcacttc acctcgaaaa gcccgtttag taattgacct tatcagaggt
    83341 aaaagtgtcg cagatgcaat ttcaatcttg aaattcacac caaacaaatc tgctggaatc
    83401 attgaaaaag tattgatgtc agcagttgcg aatgcagaaa ataactttga cttagatgtt
    83461 gaaaacttgg tagtaagcga agcttttgtt aacgaaggac ctacaatgaa acgtttccgt
    83521 cctcgtgcaa aaggatcagc ttctccaatc aacaaacgta caagtcatat cacggtagtc
    83581 gtatcagaaa aataaggagg gataatcagt gggtcaaaaa gtacatccaa ttggaatgcg
    83641 tgtaggcatc atccgcgact gggatgctaa atggtatgct gaaaaagagt atgctgagtt
    83701 cttacacgaa gatttaagaa tccgtaaatt tatttcaact aaacttgctg atgctgctgt
    83761 atcaacagtt gaaatcgaac gcgcaacagg tcgcgtaaac atttcaattc acacagctaa
    83821 accaggtatg gttattggta aaggcggttc tgaagtcgaa agcctaagaa aagaattaaa
    83881 caaattaact ggtaaaagaa tccatatcaa tatcgtggaa atcaaaaaac cagatctaga
    83941 tgcaaaatta gtaggtgaag gaatcgcacg tcaattagaa aaccgtgttg ctttccgtcg
    84001 cgctcaaaaa caagccatcc aacgctcaat gcgtgctggc gctcaaggga tcaaaactca
    84061 agtatctggt cgtctaaacg gagcagatat cgctcgttca gaaggatact cagaaggaac
    84121 tgttcctctt catactttac gtgcggacat tgattacgca tgggaagaag cagatacaac
    84181 atacggaaaa ctaggagtta aagtgtggat ctatcgtgga gaaattcttc caactaaaaa
    84241 aaacactgag aaaggaggga aataatcatg ttagtaccta aacgtgtaaa acaccgtcgt
    84301 gaattccgcg gaaaaatgcg cggggaagct aaaggcggaa aagaagtagc attcggtgaa
    84361 tacggtttgc aagctgttga ttcacattgg atcacaaacc gccaaatcga agctgctcgt
    84421 atcgcaatga ctcgttacat gaaacgtggt gggaaagtat ggattaaaat tttccctcac
    84481 aaatcttata ctgccaaagc aattggggta cgtatgggtt ctggtaaagg ggcacctgaa
    84541 ggatgggttg caccagtaaa acgtggtaaa atcatgtttg aaatcgcagg cgttcctgaa
    84601 gaagtagctc gtgaagcgtt acgtctagct tctcacaaat taccaatgaa aactaagatc
    84661 gtaaaacgtg aggaaatggg tggtgaatcg aatgaaggtt aaagaaatca gagaattaac
    84721 cactgccgaa atgcttgatc aagaaaaaca attaaaagaa gaattgttta atcttagatt
    84781 ccaattagca acaggtcaat tagaaaacac tgcacgtatt aaagaagtac gtaaatcgat
    84841 tgcacgcatc aaaactgtgt tgcgtgaaca agctaagtaa ttatggaagg aggccattaa
    84901 gcgtatgact gaaggaagaa atcaacgcaa agtttatcaa ggtcgcgtgg tttcagacaa
    84961 aatggataaa acaattaccg ttgctgtcga aacaaagaaa aaccacccta tctatggcaa
    85021 acgcatgaac tattctaaga aatacaaagt gcatgatgaa aacaacacag caaaagttgg
    85081 cgacatcgtg agaattatgg aaactcgtcc attatcagct acaaaacgtt tccgtttgtt
    85141 ggaagtagtc gaagaagcag ttattatcta ataacacgag attttcctat atagattgaa
    85201 tgcaaaatct gaaaggagga tacacaacgt gatccaagca gaaagtcgtt taaaagtcgc
    85261 tgataactca ggcgcacgtg aaattttgac gatcaaagtc cttggtggat ctggtcgtaa
    85321 aactgctaac atcggtgatg tgatcgttgc tacggttaaa caagcaacgc caggtggggt
    85381 tgtcaaaaaa ggtgacgtcg ttaaagccgt tatcgttcga acaaaatcag gagctcgtcg
    85441 tacagacggt tcatatatta aatttgatga aaatgctgca gtgattattc gtgatgataa
    85501 aagccctcgt ggaacacgta tctttgggcc agttgcacgt gaactacgtg aaaacaactt
    85561 catgaagatc gtttcactag caccagaagt tttataatct tgagacactt atcaaaggag
    85621 gtgcgaaaca gttatgtttg ttaaaaaagg cgataaagtt aaagttatca ctggaaaaga
    85681 caaaaacaaa gaaggcgttg tactagcagc gttcccgaaa aaagataaag ttattgtcga
    85741 aggtgtaaat gtcgttaaaa aacaccaaaa accttctcaa gctgctcctc aaggtggaat
    85801 tgttgagatg gaagcgccaa ttcatgtttc taacgtgatg gtggttgact ctactggtgt
    85861 agctggccga gttggctaca aagaagtaga cggcaaaaaa gtccgtgttt ctaaaaaaac
    85921 cggtgaagtt ttagataaat aaaagtttaa ggaaggaggg tcttactgaa tgaaccgcct
    85981 aaaagaaaaa tatcttaaag aagtaactcc atcattgatg gaaaaattta actatagctc
    86041 tgttatgcaa acacctaaag ttgaaaaaat cgtcatcaac atgggtgttg gtgatgcggt
    86101 atcaaacgct aaaaacttag ataaagcagt tgaagaatta actttgatca ctggtcaaaa
    86161 accaatgatt acaaaagcta aaaaatcaat cgctggattc cgtttacgtg aaggaatgcc
    86221 aattggtgca aaagttacat tgcgcggaga aagaatgtac gaattcttag acaaattggt
    86281 ttctgtttca ctaccacgtg tacgtgactt ccatggtgta agtaaaaaag cctttgacgg
    86341 tcgtggaaac tatactttag gtattaaaga acaattgatc ttcccagaag tagattatga
    86401 tttagtagat aaagtacgtg gtatggacat cgttattgta acaacagcaa actctgatga
    86461 agaatctcgt gagttgttga cacaattagg catgccattc caaaaataat taaaggaggc
    86521 gaactacgtg gctaaaaaat caatgattgc taaaaacaaa cgtcctgcaa aattttcaac
    86581 acaagcttat actcgttgcg aacgttgcgg acgtccacat tcagtttatc gtaagttcca
    86641 tctttgccgt atttgcttcc gcgaacttgc ctataaaggt caaattcccg gcgtgaagaa
    86701 agctagctgg taaaaagtaa attcgcataa gaaggaggta aaagatcaat ggtcatgaca
    86761 gatccaattg cagattttct aactcgtatc cgtaacgcaa acatggttaa acatgagtct
    86821 ttagaagtac ctgcatcaaa aattaaacgt gacatcgctg aaatcttgaa acgtgaaggt
    86881 ttcgttcgtg atgttgaata catcgaagat gacaaacaag gcgtgatccg tgttttccta
    86941 aaatttggta aaaacggcga acgtgttatc acaaacttaa aacgtatctc taaaccaggt
    87001 ttgcgtgctt atgtcaaagc tgacgaagta ccaaaagtat tgaacggttt aggtatcgca
    87061 atcatttcga cttctgaagg tgttgtcact gataaagaag ctcgtgaaaa aaatatcggc
    87121 ggcgaagtta tcgcttacgt atggtaatca agtagaatct acaaggaggt gtctttaagt
    87181 gagccgtatc ggaaataaag tcgttgttat ccctgaagga gtaacagtta ctcaagacgg
    87241 aaacaacgtt acagttaaag gaccaaaagg tgaattgact cgtacttttt cagcagatat
    87301 caaaatgaat atcgaaggaa acgaagtaac tttcactcgt ccaaacgaca gtaaagaaat
    87361 gaaaacaatt catggaacaa ctcgtgcgaa cttcaacaac atggttgtag gtgtcagcga
    87421 aggtttccaa aaagcgttag aactaatcgg ggttgggtac cgtgcacaat tacaaggatc
    87481 aaaacttgta ttgaacgttg gttactcaca tccagtagaa atcacaccac cagctggagt
    87541 aactgttgaa gtaccagcta acacacaagt aattgttaaa ggtgcgaaca aagaagaagt
    87601 tggcgaatta gctgcaaaca ttcgtggaac tcgtcctcca gaaccttata aaggtaaagg
    87661 tattcgttat gttggcgaat atgtacgccg taaagaaggt aaaactggta aataatcgtt
    87721 tagaaaaatt gcatagaagc cagcagcagg aaaaaggcat ttggctgaag tttaccttca
    87781 aaaaaataca tttttacttg tgccgcagct tctatttttc gaaacgtatt tactgcttga
    87841 aatagtagta caataaaaat aaagaggtga caattgtgat tacaaaacca gataagaaca
    87901 aaacacgtca aaagagacat cgtcgtgtac gtaacaatat ctctggtact gctgagcgcc
    87961 cacgcttgaa cgtttttcgt tctaacaaaa acatctacgc tcaaattatt gatgacgtag
    88021 cgggtgtaac gctagcaagt gcctctacct tggataaaga aatttcaggt ggaacaaaaa
    88081 cagaagcagc acaagctgtc ggtaaactag ttgctgaacg tgcagctgaa aaaggcatta
    88141 aagtagtagt ttttgaccgt ggtggatacc tttaccatgg ccgtgtgcaa gctttagctg
    88201 aagctgctcg cgaaaatgga ctagaatttt agaagaagga ggaataccat tcatggttta
    88261 tatcgatcca aaacatttgg aattagaaga ccgcgttgtt gcaattaacc gtgtaacaaa
    88321 agttgttaaa ggtggacgtc gtctacgttt tgctgcttta gttgttgtcg gtgacaaaaa
    88381 cggacacgta ggatttggta caggtaaagc tcaagaagtt cctgaagcaa tccgtaaagc
    88441 aattgaagat gcgaagaaaa acttagttga agtacctatg gttggttcaa caatcccaca
    88501 cgaagtaatc ggtgctttta gtggtggtag aatcctaatg aaacctgctg tagaaggttc
    88561 tggggttgct gctggtggac cagttcgtgc cgtattagaa ttagctgggg tagcagatat
    88621 cacttctaaa tctttaggct caaatacacc aatcaacgtt gttcgcgcaa cagttgaagg
    88681 tttgaaacaa ttgaaacgtg cagaagaagt tgcagaactt cgcggcaaat cagttgaaga
    88741 attaatcgga taaggaggac aacaataatg gctgaattaa aagttacttt aaaacgcagt
    88801 attatcggac gtcctcaaaa ccaacgcgat acagttaaag cgttaggtct tacaaagatt
    88861 aacagttcag tggttaagcc ttctaatgaa gcaattaaag gcatgatcaa tactgtttct
    88921 cacctagtgg acgttgaaga aatttaattt agaaaaatca taactagaac attaaggagg
    88981 tgccaaacct atgaaacttc atgaattaaa acctgctgaa ggttcacgcc aagtacgtaa
    89041 ccgtgttggt cgtggtactt catctggtaa cggaaaaact gccggacgcg gacaaaaagg
    89101 acaaaaagct cgttcaggcg gcggtgtgcg tttaggattt gaagggggac aaacaccatt
    89161 gttccgtcgt ttaccaaaac gaggattcac aaacgtgaac cgtaaagact atgcagtcat
    89221 taatcttgat gtattgaacc gctttgaaga cggagctgaa gtaactcctg ttgctttagt
    89281 tgaagctgga atcgtgaaaa acgaaaaagc tggaatcaaa gtattagcta atggggaact
    89341 aaacaagaaa ttaactgtga aagcagcaaa attctctaaa gcagctcaag aagcaattga
    89401 agcggctggt ggatcaatcg aggtgatcta atgtttagac tattaaaaga tgcttttaaa
    89461 gtaaaagaca ttagatcgaa aatcctattt actgtatttg tgttgtttgt atttcgccta
    89521 ggtgcacata ttactgtacc aggcgtaaat gctgcttcat tgcaaaacct ttcttctttg
    89581 ccatttttca acatgttgaa cttggtaagt ggtagtgcga tgcaaaactt ttccattttc
    89641 tcaatggggg tttcaccata cattactgct tctatcgtca ttcagcttct tcaaatggac
    89701 atcgtgcctc gatttgttga atggtcgaaa caaggggaag ttggtcgtaa aaaactgaat
    89761 caagcgactc gatatctgac attagtattg gcatttatcc aagtcggtat cactgctgga
    89821 ttcaatcaat acgctcaatt aggtttcgtg aataatccta acgtagcaac ttacgtaacg
    89881 atcggtttga ttctaacagc cggtacgatg tttgttactt ggctaggaga acagatcaca
    89941 gaaaaaggta tcggaaatgg cgtatcaatg atcatttttg caggtatcat ttctagattg
    90001 cctgaaagta tcagagaaat ttatgaagat tactttataa atattgatcc ttcagacata
    90061 tggaaatctg ctatttttgt ggccatttta ctagtagcgg tcttgttgat cgtgacattc
    90121 gtaacatttt tccaacaagc tgaacgcaag atcccgatcc aatatacgaa acgcgtagct
    90181 ggcgcgccta caagcagcta tctgccgttg aaagtaaatg cagctggagt tattccagtt
    90241 atctttgcta gttcgtttat tgctacgcca aatgctgttt tacaagcatt gagcggtaga
    90301 ttcggttctg aaaactggta caaagtgatg atggagatct ttaactataa tacagcacct
    90361 ggtgctacga tttatacgat tctgatcgtt gccttcactt tcttctacgc ctttgttcaa
    90421 gtaaaccctg agaaattagc ggaaaacttg caaaagcaag gaagttatat accaagcgta
    90481 cgccctggta aggggactga agagtatgtc tcaagattac tgatgcgttt aagtacagtt
    90541 ggtgcagtct tccttggttt agtggcattg cttccaatca ttgctcaaat ggtttgggat
    90601 cttccacaat caatcggatt aggcggtaca agcttactga tcgttatcgg tgtagcttta
    90661 gaaactgcga aacagttaga aggcttaatg ctgaaacgta aatatgttgg attcattgag
    90721 taagaaagcg tgttgaggtt tcccctcaac acacgttctc acacatttat taggagggaa
    90781 aacaatgaac ctcattttaa tggggctgcc aggcgcaggt aaaggaacac aagctgaaaa
    90841 aatcattgat gcttatggta ttccgcacat ctcaacagga gatatgttcc gcgcagcgat
    90901 gcaaaacgaa acagctcttg gcctggaagc aaagtcttat atggataaag gtgcattagt
    90961 tcctgatgaa gtaacaaatg gaatcgtgaa agagcgacta gctgaaccag atacagagaa
    91021 aggcttctta ttagatggct ttccacgtac gctagatcaa gcaaaagctc tggacgcaat
    91081 gctaaaagat ttgaataaaa aaattgatgc tgtcatcgat atccatgttg gtgaagaaat
    91141 cttggtcgaa cgtctagccg gccgatttat ctgcagcaat tgtggagcaa cttaccataa
    91201 aattttcaac cctacaaaag ttgaagacac atgtgatcgt tgcggtgggc atgaattcta
    91261 tcaaagagaa gatgataaac ctgagacggt taaaaatcgt ctggctatca acatcaaaaa
    91321 tagtgaacca atcttggctt attataaaga acaaggtttg ctaaacacta ttgatggtgc
    91381 tcgtgaaatc gatgctgtat ttgcagatgt acaaaaaatc attgaaaaat agcacgattg
    91441 cccgtagccg acttgcatta tgcagttact cttgtcaagt tcaataaatt atgatagaat
    91501 atgtcagtcc gacttctaga gaatcctcta gatgatccat tttgagaatt ttgaggtggg
    91561 aactgcagtt tccgccattt tatcgtgtga aaatgcgaaa caagtacaag gaggtactgt
    91621 gcgtggcaaa agaagatatg attgaagtcg aaggtacagt cgtcgaaact ttgccgaatg
    91681 caatgtttaa agttgaacta gaaaacggac accaagttct tgctactgtt tcaggtaaaa
    91741 ttcgtatgca ttacatccgt atcttacctg gtgacaaagt aacggttgaa ttgtctccat
    91801 acgatcttaa ccgtggccgt attacttatc gctttaaata attgtactcc gtaaaactac
    91861 aggaggtatt atcatgaaag taagaccatc agtaaaacca atgtgcgaac attgtaaagt
    91921 aattcgtcgt aaaggacgcg ttatggtgat ttgcccagca aatccaaaac ataaacaacg
    91981 tcaaggataa ttggaggtgc tataaatatg gctcgtattg caggagtaga tattcctcgt
    92041 gataaacgtg tagtagtttc acttacttat atctatggta ttggtaacac tactgctaaa
    92101 aagatcttag ctgatgctgg tgtatctgaa gatgttcgcg ttcgtgaatt aacgaatgaa
    92161 caaacagatg ctatccgtgc agaagtagat aaattaaaag ttgaagggga tcttcgtcgt
    92221 gaagtgaact tgaacatcaa acgtttgatg gaaatcggtt cttaccgtgg aatccgtcat
    92281 cgccgtggat tgcctgttcg tggacaaaac acgaaaaaca atgcacgtac tcgtaaaggc
    92341 ccagctcgcg ctatcgctgg caagaaaaaa taattcttaa gtgaaggagg ttaaaacttc
    92401 atggcagcaa aaaaagtgaa tcgtaaacgc cgtgtgaaga agaatataga agcaggtatt
    92461 gcacatatcc attctacatt caataacact atcatcatga tcactgatac tcacggaaat
    92521 gcattagcgt ggtcatcagc tggttcatta ggtttcaaag gaagcaaaaa atcaactcct
    92581 tttgccgctc aaatggcagc agaaactgct acaaaagcag caatggaaca cggattaaag
    92641 actgttgaag taacagttaa aggacctggt tctggacgtg aagcagcaat tcgttcatta
    92701 caagctacag gtttagaagt gactgcaatt cgtgacgtga ctccagttcc tcataatggt
    92761 gcccgccctc caaaacgccg tcgtgtttaa tgagtgccgc tcattttgag ttttatcagt
    92821 aacgtcattg aacaagacac ttttcgtttt gaaaggggta aagataagaa tgattgagtt
    92881 cgaaaaacca agaatcgcaa aaattgatga agaaaaagat tatggcaagt tcatcgttga
    92941 acctcttgaa agagggtatg ggactacttt agggaattcc ctacgtcgta ttttattatc
    93001 ttctttgcca ggtgctgcca tcactaatat tcaaatcgat ggtgtactgc atgaattctc
    93061 taccatcaaa ggtgttagag aagacgtcac tcaaatcatc ttgaacatta aaggcttagc
    93121 gctgaaaatg tatggccaag aagaaaaaac ccttgaaatc gatattaccg gacctgctac
    93181 agtaactgct ggcgatatta tcgttgatag tgatgtagaa attcttaaca aagatatgta
    93241 catctgtagt gtctctgaag gagctacgtt ccatgctcgc ttaacagtga aacctggacg
    93301 tggttatgtt caagcagatg aaaataaaaa agaagatatg ccaattggcg ttcttccagt
    93361 cgattcaatc tacacacctg ttcgtcgtgt gaactatcaa gttgagaaca cacgtgttgg
    93421 acaccgtgaa gattttgata aattgacgat ggagatttgg acagatggtt caattgaacc
    93481 gttggaagca atgagtttgt ctgcgaaaat catgactgaa catttggata tttttgtgaa
    93541 cttaactgat gaagcaaaaa atgctgaaat catggtcgaa aaagaagaaa cacaaaaaga
    93601 aaaaatgttg gaaatgacga tcgaagagtt agacttgtct gttcgctctt acaattgttt
    93661 gaaacgtgca ggaatcaata ctgtacaaga acttacaaat aaatctgagc ctgaaatgat
    93721 caaagtccgt aacttaggac gtaaatcatt agaagaagtt aaagcaaaat tgcacgactt
    93781 aggtttaggt ttacgtaaag aagattaaaa ccttttacga aggagggaaa ccaacgtgag
    93841 ttatcgtaaa ctaggacgca catctagcca acgtaaagcg atgttgcgtg atttaacaac
    93901 tgatttatta atcaacgaac gtattgtgac tactgaagct cgtgcgaaag aagttcgctc
    93961 aactgcagaa aaaatgatca ctttaggtaa acgcggagat ttgcatgcac gtcgtcaagc
    94021 tgcggctttc gtacgtaatg aagttgccag cgtgcgtgaa gaaaatgagg acatcgtcat
    94081 tgaatcagct ttacaaaaac tgttcaacga tattgcacct cgttacgcag aacgtcaagg
    94141 cggatacaca cgcattttga agacagaacc aagacgcggt gatggtgcac caatggttgt
    94201 cttagaactt gtctaaaatc acactaataa catcacttta tagatgattc ttttagagtg
    94261 ttatgatgtt ggaagatatt tttccgagtc tagctcagcg ctgctcctca agcgaatatc
    94321 gcttggggag taagcactca tcataaagtg tttttttata ccttcaatta cctgtcatga
    94381 cagatatgtt tgaaggcata aaaaaacatt ttaagcgaga aataattgaa aattttgtct
    94441 ttttttgata gtgttagtag gaactttagg atgaagaagg agtaatcatg gagccaatta
    94501 ttgaattaga aaagataaat tataaatacc agccagatga tcttcgcccc gcattgaaag
    94561 atgtctcctt tacaattgat aaaggtgagt ggatcgctat tattggacac aatggttcag
    94621 gaaaatcgac acttgccaaa acgatcaatg gattgcttct gccagaatca gggatcgtga
    94681 aagtgggaaa tcaaatattg gatgaagaaa atatttggac gatccgacag atggttggaa
    94741 tggtttttca aaatccagac aatcagtttg ttggttcgac agtagaagat gacgttgctt
    94801 ttggtcttga aaatcaaggg attcccagag aagaaatgct agtgcgtgtg aaagatgcac
    94861 ttgagaaagt tagaatggct gagtttgctt cacgcgagcc tgctcgttta tcaggagggc
    94921 aaaaacaacg tgttgccatt gccggcgtgg ttgccttacg acctgatatc attattttag
    94981 atgaagcaac cagtatgcta gatccagaag gtcgtgaaga agtgatctct acgatcaaga
    95041 agatcaaaga agaaagtcaa ttgaccgtta tatctatcac tcatgatatt gatgaagcag
    95101 ctaatgccaa tcggatacta gtgatgagac aaggcgaact tgtccgtgaa ggtacaccaa
    95161 aagaaatttt ttctgctggt cctgaactga tcgacttagg tctagatcta ccatttcctg
    95221 aaaaactgaa gagcgcactg aaagaacgtg gggttgatgt gcccagtgaa tatatgacag
    95281 aagaaaggat ggtggattgg ttatggacat ccgttttgaa caagtagact tcacttatca
    95341 gccgaataca ccatttgaac aaagagcctt atttgacatc aatatgacga tcaaagaaaa
    95401 cagttatact gcattagtag ggcataccgg aagcgggaaa tcaaccttac ttcagcattt
    95461 gaatgcgctt gttaagccaa caagcggtac agtccatatt ggagaacgag atattcagcc
    95521 ggatacggat aataagaatt taaagcccat ccgaaaaaaa gttggcatcg tctttcagtt
    95581 tccagaagca cagctattcg aagaaacggt agcaaaagac attgctttcg gtcctaaaaa
    95641 ctttggtgtc agcgaagaag aagcactagt cctagcaaaa gaaacattag aacaagttgg
    95701 gctggatgaa agctatttgg aacgttcgcc atttgaactt tcaggagggc aaatgcgtag
    95761 agtagcaatc gctggtgtgc ttgccatgag gccggaagta cttgtattgg atgaaccaac
    95821 ggcaggactt gatccccagg gcagaaaaga aatgatggag atgttttggc gtcttcataa
    95881 ggaacagcag atcactattg tattagttac gcatttaatg gatgatgtag ccaattttgc
    95941 agattatgtc tatgtgttgg aaaagggacg aatcgttaat agcggggagc cgcaagaggt
    96001 ctttcaaaac attgaatggc taaaagaaaa acaactaggc gtacctactg ccaccgagtt
    96061 tgcagaagaa ttaatggcta aagggatgaa ctttgctacc ttgcctttga cagctgaaga
    96121 attagcagac gctatcgtcc attatgctgg aggaaatgcc catgatgaat aaattgatat
    96181 ttggtcgtta tatgcctgga gattccttga tccatcgatt ggatccccga gctaaattat
    96241 tagccagttt ttattttatc ggaatcattt ttttagcaaa taattgggaa acgtaccttt
    96301 tgttggcatt tttcacatta ctttctgtct tcttgtcaaa agtagatttg aaatttttta
    96361 tccgaggcat tcgcccattg atctggctaa tcctcttcac tgtagctttg caggtcttgt
    96421 ttacaagtgg cggagaaact tattggagtt ggggtgtttt ccatatcact gaatttggtg
    96481 tgatgaacgg cctctttatt ttctgtcgat tcgtgttgat catttttatg tcgactctac
    96541 tcacgcttac tacaccgcct ttggaattat cggatgcgat tgagtacttg cttcgtccat
    96601 tgaaagccgt acggttccca gtccacgaaa tttcattgat gctgtcgatt gctcttcgtt
    96661 ttgtgcctac attgatggac gaaacagaaa agatcatgaa cgctcagcgc gctcgcggtg
    96721 ttgattttgg tgaaggaaac ctagttcaga aaatgaaagc agtcattcct ttgctgattc
    96781 ctttatttgt cagcagcttc aaccgtgcgg aagatcttgc gactgctatg gaagcaagag
    96841 gctacaaagg tggggaagga agaacgaaat atcgtgtatt gcattggcat aatagagaca
    96901 cgatggttat cattgctttt gtattattaa ctgtgctact gatcttttta agaggataaa
    96961 cgttcagtgg gccgctatct attcttttct tgctgatctg tttgcaagaa aggatgcaag
    97021 cagtgtatga caagaaatat tagggtctgt ggcagactaa ggtcacaggc ctctttcttt
    97081 tatctgaaaa gaaagaacga aaaaatttta tagaaaaatc attattccag ataacctttt
    97141 aggaacaaag gagagaaaaa catggtacgt tacaaagcaa tcattgcgta cgacggaaca
    97201 aattttaatg gattccaaaa acagccaaat gggcgtactg ttcaagaaga agtagaaaaa
    97261 acattacaaa aaatggcaaa tggaaaagaa atcacggtct ttggttcggg aaggacagat
    97321 gccggagtac atgcgatcgg gcaagtaatc cattttgatt atccagagga gcgtccgtta
    97381 gaacgaatgc gtttcgcgct agatacccag tcaccagagg atattgctgt aagacaagta
    97441 gaaattgttt cagaagactt ccacgcaaga tatttggtca aagaaaagac gtatcaattt
    97501 cgagtggata tagggaaacc acgcagtcct tttcgacgcc attatgcaag ttattttcct
    97561 tacccacttg atttggaaaa aattcagcgg gcgcttcctg atttattagg gactcacgat
    97621 ttcacctctt tttgtgcatc aggaagctcg atagaggata aaatacgtac gatctacgag
    97681 gctaagatgg aggtaaatga aacaaaagac gaattgttgt ttacatttag gggaaatggc
    97741 ttcttgtata agatgatacg gatcttagta ggaacgcttt tgaaaattgg caatggccga
    97801 ttgcctgaag atagtatccc agatattatt gcgaaaaaag atcgtaatgc agcaggacct
    97861 acagcacacc ctgaaggact ttatttgtat gaagtgaaat atgaataaga ggttatgata
    97921 gaagtggtca gcttcaagaa ataagaaggt atttacgaaa attacttctc aaaattttgc
    97981 aaaatttcgg cttattttcg aagaagctac ttctggaaca ccgtttaagg aagaggtcgt
    98041 gaaatcagcg ttttgacctg agtattagag aaacaaacgg gaaaatagct cctcatattt
    98101 tgccgtttgt tgagctaata ccgaaggtca gctgattgaa caccgtttaa gaaagaggtc
    98161 gtgagcctgt catttaactc tgagtcacaa agagcaattt caaaaaacag cttctcatct
    98221 ttgatgaata tcgtgacttg tggacgaggc gttgataagt gaatcatctt tttgttacct
    98281 attttttctg taaccaaaaa acgatggaac gttgttttga ttgcccttgg tgcggacagt
    98341 ctgaacaggt tccatcgttt ctttattttc tacgtcttct tctgtgtgtg tttcgtggtt
    98401 gttcttctat tgctggatca atcgtgccat aaaaggggcg attgcggtca aggtaccaga
    98461 cataaagaat aaacaatgca atacatagga caaaaaggat tgcacctatc gtaaatcctt
    98521 caggcaagta actgaaagtg acagtatgtt tacctgctgg aacttttatc atcaagaatg
    98581 cgtctttgaa attgctgacc ttgactggtt tcccatcaat tttagctttc catcctttgt
    98641 cataaggaat agttgtcacc aacatggaat cttcagcggt atttacagtc ccagaagctt
    98701 ttctgccagt ggcttccatt tcgacacctt tttctttgat ctgattcatt gcggcttgat
    98761 aggctacaac atctagacca acgactttag gctccatgaa acttatttct ttcgtcccat
    98821 aaaaacttgc agtaaatgtc actgttgtct cttgaggata atagccaaga tcatagtatt
    98881 gaccagaaat gttgatttgc gtttttcgcg atgtaccatt gacagtgatc gtcgcactag
    98941 aactttctaa ttgcgcaaaa ttggttggga acaaacttaa gtaagcttgt gtatttgcag
    99001 gcacatggac ttcccaagtg agctcttttg gctggttatc tgctgtttct gagtaagtga
    99061 cgccagtcgt tgtctttgtg atcgtcgtat tcaactgatt cttcaaagta ggtgtataga
    99121 atgtaaaata ctgttcattt agaccagaca atgtattgat caagtttgtc tgactggtca
    99181 aattatctgt cactggctgc tcaacatcat taacggacaa cggtgcagta aatgctagtg
    99241 gcaatgcatt gacattctga tacagtgtat agtcgccgct ttgaccagct tggcggaagc
    99301 catatttact gaacgtgctt tcgtttttag aaatgttata tttgattcct gtaaacccat
    99361 ccatcagcaa agtattattg ttataacgga tattcaaatt tgtccctctt gaacggaaac
    99421 ctaatttatc caggtaagag gaagaattac gatttcttat agaagaaaac aaactgatac
    99481 cactgtaacc atagttgatc gaatcgtttg cagatactgg atccagattt tccaagcggt
    99541 aaaacgagtc ggaatctttt tttgtttcat ttacaagagc tttcaagtca ggataaggat
    99601 cagtatataa actgcgggaa gcataattcc aatcatcaag aatgccatgg atcatactgt
    99661 tcgtattgac cagtgcttca gcagacatga tcaataataa caatatactc agatgattga
    99721 atgaaagttt tttatgctga taaaatccca cagcaaaagc ataaatccct aagaaaacca
    99781 ctgtcaatac aaaggaagtc atcgtcacat aggtgtagct tccttcaggt ttgatgcccc
    99841 atgccaccgc aaagagagca gctacgacca aaatgattcc taacaacagc ccacgatcgt
    99901 cttgttctat cttttcccat ccttttcctg caagcaggag cactaagaaa gagaacagat
    99961 aggcataacg gaataaaaac atattcggtg catgcatacc atgccagaac aaattcaatg
   100021 gaacaagata aaaacttgcg ataagtatgg cgaaaagact gccgtacaaa aatttatttt
   100081 tcaacggtac tttccgactg gcaaaataaa acagacagag gactaatggt agcagacctg
   100141 cgtaaacgaa cggaatcgat ccgtattttg tcgtgtcata aacaccaatc atatttttca
   100201 tgattagatc cagataagaa gtcgcttctg ttttcaacgt agtgatctga ctcagtgcct
   100261 cgccatttga ccgaagatcc agcactgctg gcaatacaat gatcattgaa gcgccgcctg
   100321 ccagtaaaga agtgatacca taaggaacaa tcgttttttt atattctgac cagtttgtca
   100381 atagacgggc gataaaatat aagaaagaaa atacgccaat cataaaaccc atataaaaac
   100441 tggataaaaa caagagtaaa taactgataa ataaaagttt cggccgttta agatccataa
   100501 cacgatgaat ccccaaaatc actaatggaa gataaaaaaa tgcatccagc cacatgatga
   100561 tctccgaatg tgcagtgata aatgagatca atgcgtaact gacgctaagt gcaacatgcc
   100621 cccagcgagg aatcttgaac gtacgtgcaa gaaaccaaaa agccaatccg gcacagccga
   100681 ccttaagcaa agtcagaaca tacagtgcat ctggcatcat ctggtttgga aaaaagaaca
   100741 ccagtggcgt gaagatcccg cctagatagt aagaaattaa agataaataa ttcaatccta
   100801 gagaagcatt ccatgtataa aaaatacttt gctttccaag aagaacattt cggaagctgg
   100861 catgaaaatt agaaaactgt gaaaaggcat cacttgccaa aatacttctg ctgcttccag
   100921 gatagattcc gatacttaga taaacaagtg ctaaaatcaa aaacggaatc aggaaactag
   100981 ctgctgcgta gcgtccgttt tctctaaaga atgactttaa attttttttc atatttattt
   101041 tccaccaaat ttctatgaag ttgtattcag tttatcatat ccttgttttt ctctgcttaa
   101101 aaagagtcta gttttcacaa aaaaataaaa tttagaataa ataacttcat aatttgttcg
   101161 tataacaaaa ataaacgcaa aaaaagggta ggaatcaaaa gtcccaccct taaaaatttt
   101221 tattaaataa aaaaccgttc actatctgaa cggtccaagt atcattcaat taaacgcgtt
   101281 caacttttcc tgatttcaaa gcacgagttg acacccaaac ttttttaggt tttccgtcga
   101341 ccataacgcg aactttttgt aagttaggtt taacagtacg ttttgttgag ttcatagcgt
   101401 gtgaacggtt gtttccactt tttgtcttgc gacctgtaaa gtaacaaact tttgccatgg
   101461 taatttcctc ctttgccttg atttggagca atagaataat tcagctcata ccagactaac
   101521 atatcatata gaatgggaat ttgcaagtac attttcatag atacaattga caattgagaa
   101581 gaataattgt aaattaaaca aggttatacc tgaaattttg caaggttttt gagtactttg
   101641 ggtaactgcc ttttatttca agttgcgcta tgataaaata tgatagaaga aaaatgggag
   101701 ttttttaccc gttaaggagg ctcattcatg gctgtaaaaa ttcaaacgtc agctggaacc
   101761 attgaaatta ccaatgatgt aattgcaaca gttgttggtg gcgccgcgac agatgtgttc
   101821 ggtgttgtag gaatggccag caaaaatcaa attaaagata atattactga gattttgcgt
   101881 aaagaaaatt actcaagagg agtagttgtc cgccaagaag ataatggtat tgctgtagat
   101941 gtttatacga ttatcagcta tggtacgaaa atctctgaag tttcccgcaa cgtccaagaa
   102001 aaagtgaaat acaatctaga ggcattatta ggcgtaactg ccaattcagt taatgtcttt
   102061 gtacaaggtg ttcgtgtact gccagactag gcaggagact ggctggtaac tcagcagtcg
   102121 atctgaagga ggattatcta ggtgagtgta acagaaataa gtgcaagcca gttccaggaa
   102181 atggtccagg caggtgcgaa tcgtctgcaa gtaaatgcag aatacgtcaa ttcattaaat
   102241 gtattccctg tgccagatgg cgatacggga acaaatatga atctatctat gactagtgga
   102301 gctaaggcag tagtagattc aacctcagaa aaagtaggag aattagcagc aatgctttca
   102361 aaaggattgc tgatgggtgc gcgcggaaat tccggcgtta ttttgtctca attgtttcgt
   102421 ggattttcta agcaaattcc agatgttgtc tcattaaatg cttctgatct tgctgcagcc
   102481 ttcactcatg gagttgaaac tgcgtataaa gcagtaatga aaccagtaga aggaacgatt
   102541 ttgacagtag ctagagaagc agcgaaagct ggagaaaaga aagcaaaaag tactgatgat
   102601 gtcgttgaag taatgacagc tgttgtaaaa ggagcaaagc gtgctttagc taaaactcca
   102661 gacttgcttc cagtcctaaa agaagtcggt gtcgtagaca gtggagggca aggactgctg
   102721 tttgtttatg agggcttttt aagtgcattg aatggagaat tccaagcgga tgaaacatat
   102781 gaaccttctc cagcagaaat ggatgatatg gtcaatgcag aacatcatcg cagtgtccaa
   102841 ggtcaaatgg cgacagaaga tatccatttt ggttactgta cagaaatcat ggtaaaaatc
   102901 ggagaaggac ctacagtcga tagtacattc gattacgaag aattccgtaa ctatttagat
   102961 ggtataggag attctttact tgtggtcaat gatgacgaga tcatcaaagt ccacgtgcat
   103021 acagaaaacc caggagaagt cttgaattat ggacaaaaat tcggttcatt agtcaaaatc
   103081 aaagtcgaca acatgcgctt gcagcatgag acgattttgg aacacgatga agaagtgaac
   103141 gagtttgcga aaaaagaaat accacgtatc ccgtatggta tcgtagcgat cgctgctggt
   103201 aaaggcgtcc aagagctgtt tgaaagttta ggcgctaatt atgttatcag tggtggacaa
   103261 acaatgaatc caagtacgga agacattgtt aaagcgattc aagaagtaaa cgcagataaa
   103321 gtgattgtct tgccaaacaa taagaacatc ttcatggcag ctgatcaagc agcagaagta
   103381 gcagatatcc cagtagcagt tgttccttca agaacgattt cccaaggatt gacagctatg
   103441 ctttcattca atgatcaagc ttctttagaa gaaaacaaag aaacgatgac agaaatgttg
   103501 tctagcgttg tgagcggtca agtcactcac gcgatcagag atacctcaat tgacggtgta
   103561 gagatcaaag aaggagactt tttgggcatg atcgatggaa aaatcctgat ctctaaccca
   103621 gatatcacag caacagcgtt aagtacacta gaacaaatga tcaatgagga cacagagatc
   103681 gtaacgatct tggcaggtga tgaaggaaca gttgaacaag caaatgaatt tgctgagaaa
   103741 ctaacagaaa aacatcctga tttggaaatc gagatccacc aaggagatca gcctgtctat
   103801 ccttatctat tttctgctga ataatttctg tgtcagaata tctttctaac acagtattca
   103861 ttcataaaag gccttcggtt agtgatcagg tatgatcgct gaccaagtcc tttttttgtt
   103921 aaaggaggga ggaaaaattt atgcgttctt tagatgatcc aatcactatg ctggcaggag
   103981 tcggagaaaa aagagcagct agtcttgcca gcttgggcat ccaaacgatt gaagacttac
   104041 tgacttatta tccttttcgc tacgaagata ttcaggaaag aaatctaaac gagatccagg
   104101 atcaagaaaa ggttactttg aaaggaatcg tagtctcgcc tcctgtgatg aatcgatttg
   104161 gttataaaaa aagccgattg caatttcgta tgatgcaaga acatgatgtg ttcaacgttt
   104221 ctttttttaa tcaaccttat ctaaaagaaa aagtcatttt atcggaggaa atcgccgtat
   104281 acggtaaatg ggatgcaaag cgtaaagcct tgaacgggat gaagatttta ggttctcaaa
   104341 caatggatga cttttcgccg atatatcacg tcaacaaatc aattcgtcaa acgacattag
   104401 ttgatctaat caaaaaaggg tttcaagaat acggcacatt gattgaagaa aatctgccaa
   104461 acaaccttgt ggagaaatat cgactgcttg accgagcttc tgctgtaaga agtatgcatt
   104521 ttccaaaaga ccacgaagaa aaccatcaag ccaaacgacg tgtcgttttt gaagagttct
   104581 ttttatttca aatgcgtctt caaggattga aaaaagcaga aaaagcagaa acgaatggtt
   104641 tggagatcct ttatgatgtc caaaaattga aacaatttac tcaaaagcta ccttttgaac
   104701 tgacaaatgc acaaaaacgt gtaacgaatg aaatctgtcg ggatcttcgc agtccgcagc
   104761 atatgcagcg acttttgcag ggagatgtag gtagcggaaa aacagtggtt gccgcgattg
   104821 cgttatacgc taccgttaca gcaggtttcc aaggtgcgtt gatggtgcct acagaaatct
   104881 tggcacaaca acatatggaa agcttgcaac agctgtttga tcccaatgaa gtaaccatcg
   104941 cattgattac ggggtcaacg aaagcaaaag aacgacgtga gctccttgaa aaactagagc
   105001 aaggggaaat taacgtcgtg atcggcacac atgcattgat ccaagatgga gtcgaatttc
   105061 agaatttagg tctagttatt acggatgagc agcaccgttt tggcgtgaac cagcggaaag
   105121 tacttcgtga aaaaggctgg cgaccagatg tcttatttat gacagcgact ccgattccta
   105181 gaacattagc tattacagca tatggtgaga tggatgtatc tatcattgat gaacttccag
   105241 ctggacgtat cccgatcgaa acacgctggg taagaacacc tcaattggac agtgtactgg
   105301 attggacgaa aaaagaactg gcaagaggac atcaaatgta tgtcatctgt ccgctgattg
   105361 aagaatcaga agcattagat gtaaaaaatg cagtggaaat ctatgaaaaa cttcgtgaac
   105421 tatttgcacc aaaatatgaa gttggcttgt tacatggtaa aatgaagaat caggaaaaag
   105481 aagtaatcat ggaagccttc aaagacaacc agatgcagat attagtttcg actactgtaa
   105541 ttgaagtagg ggtaaacgtg ccgaacgcga ccgtcatgct gatcatggat gcggatcgat
   105601 ttggattggc acagctgcac caacttcgtg gacgtgttgg acgcggaagt gatgcttcct
   105661 actgtatctt agttgctaat cctaaaaatg aactaggtgt ggaacggatg aaaatcatga
   105721 cagagaccac caatggtttt gtactaagcg aaaaagatct ggaattacgt ggtcctggtg
   105781 aagtatttgg cttccgtcaa tctggtctgc ctcaatttgc gattgctgac ttggtgacgg
   105841 atggaaatgt tttggaggta gccagagaag aggctgcagc cctttggcaa ataaaggatt
   105901 ggcagatgct tcctgagtat cagcctttag cgcaaagcct ccaaatagaa aatcaggata
   105961 atcgttattt cgattagcag actgtgctat actttatact gatgacggaa aaaggagtgg
   106021 aaacaatgaa aattgccgta gatgcaatgg gcggagatca cgcacctcaa gcaattgtag
   106081 aaggcgtggc tttagcgcag caagattttc cagaagtaga attcttactt tacggaaaag
   106141 aagcagaaat caagaaatac ttaacaaatg aaaaaaacat cacgattatc catacagacg
   106201 aaaaaatcaa tagtgatgat gagccagtaa aagcaattcg acgaaaaaag actgcatcaa
   106261 tggtccttgc tgcacaagca gtgaaaaacg gtgaagcaga tgctgtattt tcagcaggga
   106321 atacaggcgc gctactagca gcagggttgt tcatcgtagg acgtatcaaa aatatcgaac
   106381 gccctggctt gatgtctacc ttgccagtag tagggaaaga aggtgccggt ttcgatatgc
   106441 tggatcttgg cgctaacgca gaaaacaaac cagaacattt gttgcaatac ggtattttag
   106501 gttcttttta tgcacgtaaa gtccgaggta tcggacgtcc tcgtgtcgca ctcttaaaca
   106561 acggaacaga agcaacaaaa ggtagtgaag tgacgaaaaa agcctatgaa ctccttcaga
   106621 atgaaacaag cttgaatttt attggaaacg tagaagctcg tgagctactc aatggtgtgg
   106681 ctgatgtagt ggtcacagat ggatttacag gcaacgctgt tttgaaatct attgaaggaa
   106741 cagcaatgaa tatgatgaat ctattaaaat ctgctattct aaatgaaggg atcaaaggaa
   106801 aaatgggcgc tatgctttta aaagacggct tacgttcttt gaaagcagaa atggattatt
   106861 ccaaacatgg cggtgccgtt ttgtttggct tgaaagcacc agtcattaaa actcatggag
   106921 caacaggccc cgatgctgtt cgctatacga tccgccaaat ccacacaatg cttgagacag
   106981 atgtagttgg acaattagtc gaacaatttg aaaaaagcga agaatagtat ttggatatct
   107041 gaagcgatcc gttccattga aaaaaaggtg gacattcctg aaaaatgcca gtaaaattag
   107101 ccatggtgta ggtaattgac aagtgtatga ttcttggagg tgaaaaagtt gactcgcgaa
   107161 gaagtattta acaaagtagc gaaaattatt tcaaatcatt ttgagataga taccgataaa
   107221 gtgacggatg aattgaacat caaagatgat ttaaaagctg attcaattag tattatggaa
   107281 ttcgttttag agcttgaaga tgaattcggt acggaaattt cagatgaaga cgctgaacaa
   107341 attgaaacag tcggtggcgc agtagattat atcagcagcc atcttaaata atttcaaata
   107401 aaagcaaaga aggataaaaa gctgcatgca gctttttatc cttctttttt taatcttttt
   107461 gacacgtaaa agtcattttt ctgataattc tggcgataaa cagaaattat gttgcaaagt
   107521 aaagcggttt tctatataat tattaataat tcattacaca aagataagaa taacctttgt
   107581 tataaaacga tttattctgt gtaatgtcct tttttattaa gaaaaacttc catttaggaa
   107641 gcagagaggg gtattcgatt gtccgataac caattattag aagttcagaa cctacataca
   107701 ggattccgac tcaaagatga ctattacgat gcagtagatg acgtttcatt tactttagac
   107761 aaaaacgaga ttttagcaat cgttggggaa tctgggtgcg ggaaaagtac cttggctact
   107821 acgattatgg gattacttga tcctaataac acaaaaatta caggtgagat tttatataac
   107881 gatctgaatt taatcgacct gaacgaaacg ctctataata aaatccgcgg aaatgatatc
   107941 ggaatgattt tccaagatcc gttgtctgca ttaaatcctc tcatgagaat cgaagaccag
   108001 atcaaagaag gactttccta tcacacaaag atgaatgcag aacagcgtca agctcgtgca
   108061 ctagaattgc tagaacaagt agggattcct aatcctgcga gagttggacg ccaatatccc
   108121 catgaactat caggcggtat gagacaacgt gtcatcatcg caatcgcaat cgcctgcaaa
   108181 cctccaatcg tcattgcaga tgaaccgacg acagccctag atgtaaccat ccaagcacag
   108241 attcttgatt tactgaaaga ccttcaggaa gaaacaaaaa cagggatcat cttgatcact
   108301 catgacttag gagtcgtagc agaaatggct gatcgagtgg cagtgatgta tggcggacag
   108361 ttcgttgaag tagcaggtgt aaaagaactg tttgatcatc ctaagcatcc ttatacacgc
   108421 tctttactga actctatacc acaagaagga aatcatgatg cagaactcca tgtgatcgaa
   108481 ggtgtcgttc cttctctgaa aaacatgcct cgtaaaggtt gtcggtttgc accacgtatt
   108541 ccatggatac cagcaagcga tcatgaagaa gagcctatcc ttcatgaagt cgcaccgaac
   108601 cacttggtac gttgctcctg ctataagcat ttccacttta gagacgaaaa aggagaggtg
   108661 tagatgacag aattaatcac aatcaaagac ctgaaagttc attaccctat tcgcagtgga
   108721 ttcttcaatc gagtaacgga tcatgtatat gctgtagatg gtgtggattt tattatcgaa
   108781 aaaggaaaga catatggttt agtgggagag tctggctctg gaaaatctac tacgggaaaa
   108841 gccgttgtgg gattagaaaa ggtaacagcc ggacaaatca tgtatgaagg aaaagatgta
   108901 acgaagcgaa gcaaccgcaa aaaaatgggt tataacaagg atgtgcagat gatcttccaa
   108961 gattcaatgt ccagtttgaa tcctaaaaag cgagtgttgg atatcattgc tgaacccatc
   109021 cgaaattttg aacggttaag cgatcaagaa gaaaagaaaa aggtaaaagg actgctagat
   109081 atcgttggga tgccggaaga tgcgctatat aaatatcctc atgaattttc aggcggacaa
   109141 cgtcaacgtc taggagtagc ccgtgctgtt gctaccaatc caaaactgat cgtagccgat
   109201 gaacctgtat ctgctctgga tctatctgtt caagcgcaag tattgaattt catgaaacga
   109261 atccagcaag aatacggcct cagctattta ttcatttcac atgatttagg tgtagtaaaa
   109321 cacatgtgcg acaatatcgc aattatgtac aaaggtcgat ttgtagaaat cggtacaaga
   109381 gaagacattt ataatgaccc gcgacatatc tatacgaaac gtctgctttc agctattccg
   109441 caaatcgatg tagaaaatag ggaaagccac aaagaaaatc gacgaagagt agaacaagag
   109501 tatatccaaa accaaaaaga atactatgat gcgactggtc gagtctacga tttgcgtacg
   109561 atcacaacta cacacaaagt ggcgttgaaa gatggaggtg caagctaatg tggaaaacga
   109621 ttctgcgacg tgttttattg atgatcccgc aagttattat tttaagtgtt ctgatcttct
   109681 tattagccca agcgatgccg ggggatccat ttaccggttt gatcaatcca aatcaggatc
   109741 cggcagtgat cgaacagatg cgacaagcag ctggcttgaa tgacccatgg tatgtccagt
   109801 acttccgttg gatcggaaat gctttgcaag gcgattttgg tcaaagtttc ttatacaaac
   109861 gctctgttgc tgacttgatc ggcgaacgga tcatcaacac tttatatcta tcgatcttga
   109921 cagttgtcct tacttatttg attgctgtgc ctctggggat gcttgctggc cggtatcaaa
   109981 attcaatctt ggataagatt gtcgtaatct acaacttctt tagttttgct gttcctttat
   110041 ttatcttcgg cttgatcatg ctatttgttt ttggttatcg tttaggctgg ttcccaacaa
   110101 gtggatcaca aacactaggt ttgcaaggtg gatttatcac tcaatggata gaccgtttga
   110161 aatatattct gttaccatca attacccaag catttttggc gactgccgtc acaatccaat
   110221 atctgcgaag tgaagtaatc gattcgaaat cgctcgattt tgtcagaaca gcccgttcca
   110281 aaggggtacc gacgaataaa gtattcagtc gacatatttt ccgaaatgca gcgttgccga
   110341 tggcttctca aatggggtat gaaatcactt cgttgattgc tggttctgta gttatcgaaa
   110401 agatttttgg ttatccggga gtcggaaaac tatttattga ttccatcggc caacgggact
   110461 ataccgtgat cacatcctta gtattgattt taggaatcgc gacgctagtg gggacattac
   110521 tttcagatat catcatgagt atcgttgacc cgcgtattcg gattcaataa aaccaatcag
   110581 aaaggagaat ggatcgatga gtgaaaaaaa caatacagca gttcaagaaa gtattcctcc
   110641 tatggggata cgaatgattg ctcgagaatt taaaaaagat aaaattgcta ttttttcatt
   110701 aggtttgcta gtggttttat tattagtgat ctttatcggt gcgatgctga ccgatcaaga
   110761 caaagtgatg tatgttagta tcttcgacaa atacgctgca ccaggaacag tttcgacaca
   110821 aggacaaaag ttcattctgg gagcagatga aggcggacgt gatgtattag gacaattgat
   110881 cataggtgct agaaactctg ttacgatcgg atttgcgatc acgatcctta cttcgatcat
   110941 cggggttgga ttagggatca tctctggcta ctacggaggg atcattgaca acatcttgat
   111001 gcggatcgtt gattttatca tgattctacc gatcatgctg atcattatcg tcttcgtatc
   111061 gattatttcc agatacaatg tctggtcctt tatattcatc atgagtgcat tttattgggt
   111121 agctaaagcc cgattattcc gaagcaagac attatctgaa gcaagaagag attatgtcag
   111181 tgcctcaaaa acaatgggaa ccaatgattt taaaatcatg ttccgcgaaa tcatgccgaa
   111241 tctaagttca ttaatcatta ctaatttgac gatgaacttt gcggcaaaca tcggtatcga
   111301 gacaactttg actttcttag gtttcggctt gccggcaaat gtacctagtt taggtacatt
   111361 gatcggttat gcaagtaacg gagaagtctt agtgaatcgg caatggatct ggttacccgc
   111421 atcaatcctt attttagttt tgatgttgag tatcaattat gttggacaag cattcaagcg
   111481 ttctgcagat gcgcgccaac ggttaggcta gtatagaagc gaagggggaa ataggaatga
   111541 agaaaacgat ttttggattt gcagcgctga tgtctgctgt cgttcttgca gcatgcgggg
   111601 gcggaggtac agatacctct ggaaacaact ctggaggcaa cggcaataac aaagaggaat
   111661 taacttttcc aattgcaaca aaaaataatg atgatgcaat cgaagacggc gaattagaag
   111721 ctgcggtcgc aacggacagt cagtttaaag gattgttcca gtgggagttt taccaagatt
   111781 catatgatgc agcttttatg gctccttctc atgaaccgct atttgcaaac gatgacgatt
   111841 tcagaatcac agacgacgga gctgcaacgc ttgatctaga tgaagatacg aaaaaagcga
   111901 caatcaaagt aaaagacaac gtcaaatggt cagatggtga agatgtaacg gcggacgatg
   111961 tgattttccc ttacgaaatc attgggaata aagattacac tggtatccgt tatgacgaca
   112021 ctttccgtaa catcgtaggt atggaagaat atcatgatgg aagtgccgac acaatctccg
   112081 gaatcaaaaa aatagacgac aaaacagttg aaatcgaata taaagaagta aacccaagca
   112141 tgcttcaatc gggtggtggt atctggtcat atgcagcacc aaaacatacg ttagaaggtg
   112201 tagcaatcaa agatatggaa tcaagcgatc aagtacgtaa aaatccagtg acttttggcc
   112261 cttattacat gagtaatatc gttgcaggag aatcagtaga atacttgcca aacgagcact
   112321 actggaatgg tacgcctaaa ttgaaaaaga tcacgatgaa atctgtaccg atggcaagtg
   112381 caactgaagc attgaactct aaattatatg atatgatttt ccaaatgcca acagatactt
   112441 acgataccta taaagaaatt gctggctata caaacttagg ccgtgaacaa acttcttata
   112501 cttatctagg attcaagtta ggtaaatgga atgcagatac aaagagtgtg gaatacaatc
   112561 cagattcaaa aatggctgat aaatcattac gtcaagcaat gggctatgca ttagataata
   112621 atgcagtagg tgaacgtttc tatgcaggtt tgagaagcaa tgcgacttca ttgattcctc
   112681 cagtattcca gtcataccat gatgatgaga tgaaaggatt cacacaagat ctagacaaag
   112741 cgaaaaaact attagatgat gctggctaca aagatgtgga tgacgatggt ttaagagaag
   112801 acaaagatgg aaataaacta acaatcaact ttgcttcgat gtccggtggg gaaacagcgc
   112861 aaccattggc agattactat atccaacaat ggaaagaaat tggtttggat gtccaataca
   112921 caacaggtcg tctgatcgaa ttcaattctt tctatgataa attagaaaac gatgatcctg
   112981 aaattgatgt ttaccaagct gcttggaata caggaactga tccaaatcca agtggactat
   113041 ggggaccaaa tgctgcgttc aactacactc gttatgaatc agaagaaaat acaaaattga
   113101 tcaacgatat cagttcaagc aaagcatttg atgaagagta ccaaaaagaa gcatatcaaa
   113161 aatggcaaca atatgcattt gaagaagcat ttgcgattcc aacgttgttc cgtaatgaag
   113221 tattgccagt caacgaccgt gtcaaagact gggattggtc ttatgatgta ccaaacccat
   113281 gggcaaatgt ttctgttaca gcagattcac gttcataatc actaaaaatg accgaaaaag
   113341 ggcgatgtcc tttttcggtt ttttgatatt tttctgttta tttctctaat attcaggtca
   113401 aggcgctgat ttcatgactt ctccaataaa cggtgttcaa agtccagttt ctcggtatta
   113461 agtcaaattt atgcaaaaat atggaaagcc attttcacaa aattttttct tcatactcga
   113521 aactaaacga atttttcaca acttctcgtc aatcactagg attattaaag ctttacttac
   113581 cttttgaccg aaccatttca aatttgacaa aaaaccagta taataaggaa gtatcgaaaa
   113641 aatatccata aaaggaatgt ggaaaatgga caaccagctg acaaaagaac taaaagaaaa
   113701 ctataacatc gtttttcacg atctaaattt gcttgaacaa gcttttaccc attcatccta
   113761 tgtgaatgag catcgcaatc tgcaattatc ggataatgag cgacttgaat ttttaggaga
   113821 tgccgtttta gaactgatgg tttctcagta tttattccgt ttatatcctc atttgccaga
   113881 aggaaaattg acaaaaacac gagctgcgat cgttcgcgaa gacagcttgt cgaaatttgc
   113941 caaagaatgt cactttgatc aatatgtttt gttgggcaag ggagaagaga attcaggagg
   114001 acgtacgcga ccagcattgc tttgcgacct attcgaagct tttttaggcg cgttatatct
   114061 tgaccaggga ttcgacacga cccatgcatt catcgaaaaa gtgatttttc ctaaagtcaa
   114121 agcaggtgct ttttcacatg agatggatca taaaacaaaa ctacaagaag tcttgcaaaa
   114181 atcaggtgat gtatcaatcg aataccgatt gatcaatgaa gaaggtccag ctcatgaacg
   114241 agtattttgg atcgaagttt atgtagatga tcaattgatt ggcactggac aagggaaatc
   114301 gaaaaaatta gcagaacaag ctgcggcgga aaatgcattg gcagcactgt aaaagtaagt
   114361 gagggaaagc tttgtatttg aaacgaattg aaatagccgg ttttaaatca tttgcagaca
   114421 agacagtcat tgattttgaa aatagcgtca cagcagtggt tggaccaaat ggcagcggaa
   114481 aaagcaatat tacagaggct atccgctggg tattaggaga acaatctgct aaaagtctgc
   114541 gaggcggaaa aatgccggat atcatctttg ccggttccga tacccgtaag cagctgaata
   114601 ttgcagaagt cacagtgatt ttagacaata gtgatcatta tctgccattg gagtacaatg
   114661 agatcagtgt aacccgccgt tatcgacgga ctggggaaag cgagtttttc atcaataaac
   114721 aaagctgcag attaaaagat atccaagaat tgttcttaga ttctggatta ggaaaagaat
   114781 cgttctcaat catttctcaa ggaaaagtag aagcgatttt cagtagtaaa ccggaagacc
   114841 gtcgtgggat ctttgaagaa gcagctggtg tcttgaaata caagcagcgg aaaaagaaag
   114901 cagaacagaa gctttttgaa acagaagaca acctcagccg cgtccaagac atcatccacg
   114961 aattggaaga acagttaact cctttggccg cccagagtga ggcagcaaag gagtttttac
   115021 ggctaaaaga aacactgact caaaccgatg tctctttgat ggttgctgaa atcaagacag
   115081 caaaaaaaga ttgggacaac aaacaagcac agcttgcgaa attcaatcta gaattaggca
   115141 aactatctga gtcgatccaa gaacaagaat ccattttggc gaagcagcgt aaagaaaatg
   115201 cgcaagccga tcgtctgatc gaaaaaaatc agcaagtatt attagatctt tcggaaaaac
   115261 tgaagcagac agaaggacaa aaagatgttt tgcaggagcg gacaaagcat acacaaaaaa
   115321 gctcgcaaga atatcaaact tcattagcag aagcacaaaa aaaggttaaa cattttgaaa
   115381 agcttcaaga aagcttgatg aaagcagcag cagaaaaaga aacagagatc caaaaagctg
   115441 aagcaaatct cataaaaaca cagcaagaat tagaaaaata ccaaaaatca acgaaagaat
   115501 tattggcaga attaagagat caatatgttg atttgatgca agaacaagca gctgtaggaa
   115561 acgaactgaa gtatctagaa cgtcaatata tacaagaaac agcaaaaagc aagcagacgt
   115621 tagccaagca atcggaagta gaagcatcag tagaccgatt gatcttacaa aaagaagaat
   115681 tgacacaaaa gcaggcgcag ctaaaatcat ctttaactga aacaaaagag aagttagaaa
   115741 tgatccaaca aaacgggaaa aagttccaag aagcattagc aaaagaacag cctaaaatgt
   115801 atcaattaat gaatcaagtc cagcagctac gtgctcgtaa gaaaagcctt caagaaatcc
   115861 aagaaaacta ttttggtttt taccaagggg ttcgattggt cttgcagcat aaacagcagc
   115921 tttcgggtat tgtcggagca gtggctgaac tgatcgatgt tcctgcagat ttcacgttag
   115981 ctatcgaagc agctttaggt ggggcagccc agcatgtcat cgttgaaaat gaaaaagatg
   116041 ctcggcaagc catcacgtat ttgaaacagc aaagaggagg aagagcaaca ttccttccac
   116101 tgacgacaat caaaccaaga caattaccgg ctcatatatt gacacaagca gcagctgtag
   116161 aagggtttat agggattgcc agtgaacaag tcagttatcc cgatcaaatc caaactgtgg
   116221 ttcaaaacct gctaggaacg atccttttag ccaaagactt aactagtgcc aatgcaatcg
   116281 cgcaaacgat ccgctatcaa tatcgagtgg tttccttaga aggtgatgtg atgaatgccg
   116341 gaggatccat gacaggtgga gcgaataaac gaggaaacca aggcagtttg tttgtacaaa
   116401 atcaagaatt gaaacagttg actagtgaat ttgaagaagc agataaacaa ctccaagcac
   116461 aagaaaaaaa agttcaggag ctgcaacaag aaacagctcg tttagccgaa gaacaagaag
   116521 ttttgcgtac acgaggcgaa caattacgtt ttgaagaaca agaagcgaca aatcagctac
   116581 aaaacatcat aaatgaactt gaacgttttg aaaaagaaaa gcaaatctca acttttgaga
   116641 cacgtgaact gcagcagttt atcgaagatt acgaaaagca aacgaacgaa ttgaaagaca
   116701 aacaaacaga tcttgaatcg caacgacaaa aaatcgacga agaaatcaaa tcgttaagtc
   116761 aagaaagcga ccagatggaa gccagaagag cacaagtaca aagtcaaaaa gcacaagaac
   116821 aagcagatct tgctgtcttg aaagagcagt ttaatcatct gcagatccag ctccgcggtg
   116881 cccgagtcca aaaagcagag gcaaccgaac gtcaagaagc gatagaaaag cagttggcga
   116941 cattgacagc agacttttct gatcatgaag tcacagaaga aagtttggaa aaacaaatta
   117001 acgaattatc tgctcagcga gaaacattaa aagccgagtt ggcaaaagcc aaagagcagc
   117061 gagatagaag gcaaaaggag atcgataagc ttgaagcagt cttggcagag cgtaatcgtg
   117121 agcaaaaagc acgactgtcg gaacaatcca aattagaagt ccaaaaagac cgagctgaaa
   117181 tgattttaga taatcatttg acctatctgc agtcagaata tcagctgaca tttgagaaag
   117241 cttcccaaga ctatcaagaa acaaccgata tcgaagacag tagaacaaag gtttcatcct
   117301 taaaagaaca gattgaaaaa ctgggaccag taaacttaaa ttcaatcgaa caatacgaac
   117361 aagtcagtga aagacataca ttcttagcaa cacagcgaga tgatctgtta gcagcgaaaa
   117421 accaattgtt tgaaacgatg gatgagatgg atgatgaagt gcggacaaga ttcaaagaag
   117481 tatttgaagc tatccgtcaa gaattcaaaa ttgtctttcc aaatatgttt ggcggcggta
   117541 aagctgaatt agtactgacg gaaccaactg atttgctgaa aacaggtatt gagattgaag
   117601 tacaacctcc tggcaaaaaa ctgcaaagtc taagtttgct ttctggagga gaacgagcac
   117661 ttacagccat cgcgttgctc ttttccatta tccgtgtctg tcctgttccg ttttgtatct
   117721 tagatgaggt agaagcagcg ttagatgaag ccaacgtgaa acgttttggt cgttacttga
   117781 gcgatttcca agatgacaca caatttatcg tagtcacaca ccgcaaagga acgatggtcg
   117841 cagcagatgt actgtacgga gtgacgatgc aggaatccgg tgtatcaaaa atcgtttctg
   117901 tacggatgga agatatcaat gaagaggcga aaatcaacga aacgaaagtg taggaacgaa
   117961 aatgatcaaa ttagtagcaa tcgatttgga cggtacatta ttaaatgaac aaaagctgat
   118021 ttcagatgag aataaacaag cattagcaca agcaaaacag caaggggtca agatcgttct
   118081 atgtacagga cgccccttag ctgcgatggc gcattatctg caagaattag gactggtaga
   118141 tgaaggtgac tacagcatca ctttcaacgg cggtttggta caaaaaaatg atactggtga
   118201 aatcatggaa aagaaagtga tggaagtatc agatatccaa cgcttataca aactggctca
   118261 gcaattggat ctgccactag atgttttgtc tgacaatgtg gttttacagc tgcctagtgc
   118321 cccaaggaag caatccttgt acaatgtgtt gaataagctg ctgcaattcc aaccagcgga
   118381 tataagcgat attacagatg aatttgtgct taataaggca gtaatcgctt atgaccaaga
   118441 agaattagat cctaaaatca aagaaatacc agcagaattc catgaaatgt atgagatcat
   118501 caaaacaaga agtatgcttt tggaatttat gccaaaaggt gttacaaaag cttatggtat
   118561 ttctttattg gcaaaagatc ttggtttgaa acaagaagaa atcatggcga tcggtgatga
   118621 ggaaaatgat ttaccgatga tccaatatgc tggactaggc gtagcgatgg agaatgctgt
   118681 gcctttcgtc aaagaagcag cagatttcgt gacagccagc aatgaagaaa atggcgtagc
   118741 caaagtgatc caaaaatttg tattagagta gggggagtca aatgggattt tttgataaaa
   118801 tcaagcgtgc ttttactggc gaagagaaaa cagaagaagt tgaagtacag gagaagtacg
   118861 acaagggttt agaaaagacg cgtaaaacat ttggtgaacg gatgaatgag ttgtttgcca
   118921 attttcgaac agtcgatgaa gacttttttg aagaattaga agaaacactt atcggtgcag
   118981 atgtaggttt tgaaacagcg atacgaatca ccgaagcatt acgtcaagaa gtgaaactca
   119041 gaaatgcgaa aaaatcggca gaagtccaaa atgcgatcat cgagaaactt gtggatctct
   119101 atgaagcgga aggaatcaat gaagtaaatg aactaaatat ccaagaagac ggactaacag
   119161 ttattttgtt tgtcggtgtt aatggtgtag ggaaaacaac aagtattggt aaacttgccc
   119221 atcagttcaa aaacgaaggt aaaaaagtat tgatggcagc agcagatacc ttccgcgctg
   119281 gagcaatcga ccagttagtt gtctggggcg aacgcgctgg tgttgaagtt gttcgtggca
   119341 atgcaggagg cgatccagca gctgtcgtat tcgatgcttt ggaaagagca aagacagagc
   119401 aggcagatgt tttactagtt gatactgcag gccgactgca aaataaagtg aacttgatga
   119461 acgaattaga aaaaataaaa cgagtaatcc agcgggaatt accagaagca ccccaagaaa
   119521 cacttcttgt attagatgca accactggtc aaaacgcgat ggttcaagca aaacaattca
   119581 aagaaacaac agatgtgacc ggtcttgttt tgacaaaatt agatggtaca gcaaagggcg
   119641 gtatcgtact tgctatccgt aatgaattgc atttaccagt gaaattggtc ggtcttggtg
   119701 aaggaatcga tgacttggag ccgtttgatc caaatgattt tgtcgttgga ttgttcaaag
   119761 gattactaaa agaagaataa cccatgtcta aaaagcagga gctgtgacaa aagttgcagc
   119821 tccttgtttg aatgattgtt tttaacatag ctgtgttttt gatgccttca tttttatact
   119881 tatttgtaag cggttctcaa acaatttcgt tctttttctt agactttttg attataatta
   119941 gattcatatg aattggaggt cacttaatga tgaattttaa gcaattatta ttgcatgtca
   120001 atgcgcgccc ttttatcgac caagctcgat ttggtataga acgggaagga cagcgggttg
   120061 atcttgcagg aaatctagca aaaaccgatc atccagcaat ctttggcgat cgatcctatc
   120121 acccctatat ccaaacagat tttagcgaaa cacaaacaga gatgatcacc cctgttaccg
   120181 attctattcc cgaattattt cagtatctcg ctgctgttta tgatgtgact gctcgttcta
   120241 taccgaaaga agagatgatc tggccattaa gtatgccacc tgccttaccg gaaaaagacg
   120301 aagagatcat tattgcaaaa ttaaaaaatt tcgaagatgt cttgtatcga cgttatttag
   120361 caaaagaata cgggaagcga aaacagatgg taagcggtat tcatttcaat tttgaatttg
   120421 gcgatgagtt gctaagaaca ttgttcagcc atcaggaaga atttcaagat ttttccgaat
   120481 tcaaaacaga actttatttg aaaacagcga gaaactttat gcgctatcgc tggatgatca
   120541 cttatttatt cggtgcttca ccaatgagtg agaagaacta ctttttagat gaatcacatc
   120601 cgcaagaacc tgttcgcagt attcgcaaca gtgcgctggg ctatacgaat catccaaatg
   120661 tgaaagtttc ctatgcttct atgaaacagt atttagcaga tatcgagcga atgatcgaag
   120721 aagggaaact ttcggaggag aaagaatttt atacaccgct tcgtttccga ggcggaaaga
   120781 aagtcgcaga tttagcaaca acaggtgttc gttatattga attgcggaat atcgatttga
   120841 atccttatgc aagattgggt atcaatccag agcaagttcg gttcttacaa ttattcctga
   120901 tgtacatgtt atggacagaa gaaaaagaag actgtgacca atgggtagca gaaggtacga
   120961 ctcgaaataa caaagtagca ctagaacaac catcagatca aacagaattt catcaagaag
   121021 gtagagaaat tcttgaagga atgaagcaaa tgctggttga attggattgg ttggattctc
   121081 tttatttagt agaggaagca ttgactcaaa tggatcatcc tgaacaaacg ttagcagcga
   121141 aactctatca agaagcacag ctatcgagtc agcaagaagt tgctgtggca ttgggacatc
   121201 aatattataa agaaagccat gaacgtccct atcagttggc cggttttcgc gaaatggaac
   121261 tttctactca gatatttatg ttcgatgcca tccaaaaagg tgtccaagtc aaagtattag
   121321 atgagtcgga tcaatttttg cgtctgcaat tccaagacca tgtagaatat gtgaaaaatg
   121381 caaacatgac aagtaaggac agctatatcg tcccacttat catggaaaac aaaacagtaa
   121441 cgaaaaaagt cttaaaagaa gctgggttcc gggtaccagg aggcgcagaa ttttcatcca
   121501 tggaagaagc ggtaaaagct tatccgagat ttgccgagca ggcatttgtg atcaaaccta
   121561 aatcaaccaa ttacgggtta ggtatcacga ttttcaagga aggagctagt ctggaagatt
   121621 atcaagcagg tttagccatt gcttttcgtg aagacagttc ggttttagta gaagaattta
   121681 tgccgggaac ggaatatcgt ttctttgtga tcgacggaga agtccaagcc atcatgttgc
   121741 gagtccctgc taatgttatt ggtgacagta tccgaacagt gaaagaactt gttgaagaaa
   121801 aaaatagtga ccctttgcgt ggaacgaatc atcgtgcacc tttagaattg atccaattag
   121861 gtgaattaga acaattgatg ttgaaagaac aagggttgac gatcgaatca gttccccaag
   121921 ccaatcaaat tgtttatcta agggagaact caaatatcag tacaggtggt gattcgatcg
   121981 atatgactga tgaattttct gaagcttaca aaaaaatcgc cgtttctgcg gtagaagcac
   122041 taggagctaa gattagtggt atcgatttga ttataccaga taaagaaatc gatcctacaa
   122101 ctgataaaaa agcatatggg atcatcgaag caaatttcaa tccagccatg catatgcatg
   122161 tttatccttt tgcaggaaaa ggcagaagat tgacgatgaa tgtattgaaa ttgttatatc
   122221 ctgaagtatt ttaaaagaaa aagcttttga cagattgtcc tatttgatgt caatctgatc
   122281 aaaagctttt tttgcatgta aaaatttatg tgttttttcg ttttaaaata aacgatccca
   122341 gaagcttttt ttcttttttg gtttttcttt ttcagctggc gaagagtttt cttctgggta
   122401 ttttttttcg atatcaatca caggttcatt tactgcttct tttgctgtta agagcaatcc
   122461 gaattcctca ggatttgcag tgacgtaatc gttgactaca gtgaaacggc tattgttttt
   122521 cgttgctagc tggatatatt ggttttggac agaaaggggt agagccccat tcaagagtat
   122581 ggaagcatct ggatgttttt tgagttcttt taaaaagttt tctttgtttt ttggttggat
   122641 catttcagca acagtcatac taagatagca gcgttccctg aatgtcccta aatatttatg
   122701 ctgttcttca gggttcacta aaggtgtacc ataaagtccc ttatccaaat ggctttttac
   122761 atcatcttgt gccataaaaa ataccctcct tcttctcttt gtctttctta cgattatagc
   122821 atattcccaa taatacctaa aacatatgaa aaagcggttg cttttgttca acgattcact
   122881 taaaataaga atgaaaatca ttctcaatta gaaaggtgga ttggatgcgc tacggacgac
   122941 tagtagcatt attagttatt ttaagtagta tttcattgtt catcggagta tcgaatgtac
   123001 aattgtcaga actgctccag ttagatgggc aaagcttaca aatcttgcta gtgactcgtt
   123061 tgccacgaac gatttgtttg atccttgtgg gagcgaccag tagtatttgt gggttgatca
   123121 tgcaacattt gacccaaaat aaatttgttt caccaacgac tgcgggcacg atggatagtg
   123181 cgcgtttagg tattttagtg gctatgattt tcctacctgg tgcatccttg attgtccgat
   123241 ccttgacagc attttgcttt gcttttgcag gaacattgct ttttttatcc cttacgcgat
   123301 tgttcctcca gaaaaatcaa ttgatgcttc cgttgattgg agtcatgtta gggaatatca
   123361 ttggttcagt tgctactttt tttgcttatc aattccagtt ggtacaaaat atgacttcat
   123421 ggcttcaagg gaattttgcg actgtgatac gaggaaatta tgagttattg tatgctgttg
   123481 ttccattgtt gctcttggtt tggctatttg cttaccgatt tacgatcatt ggtctgggcg
   123541 aatcgtttgc agtaaatctg ggaatagatt accagaagat gcagttttta ggcattggct
   123601 tagtatcatt ggctagtgca gtcatgttga tcatggtagg aagcattcct tttatcggag
   123661 tgatcattcc gaatcttgtc tctagaatat atggagatca agtgcacaaa acaattggcg
   123721 taactgcttt agcaggtagt ttgttcttag tattttgcga tcttgtcgct cgcgtgttga
   123781 tttttcctta tgaaatacca gtcagtgtga tcgttggaat catcggtggg gctatcttcc
   123841 tttatcttct gataagggga cgacgatatg cttaaaaaaa agccatctat ttttattggt
   123901 atgcttgttc ttgttttatt tgttacagga atgtacttga cgatccatac atatggaaac
   123961 tggcattttg catgtgcatt gaggagtaag aaaatttttg catttatcct tgtctcgcta
   124021 gctgtcagca gtgccacaat cagctttcag acaattacgc ataaccagtt tttgactcct
   124081 tcagtattgg gacttgagtc gttatatgtc cttgtccaaa cggtcctttt cttctttatc
   124141 ggaggagtga cgatgcttca gcagacaggt acagtttttt tcttattgaa cgttgggctg
   124201 atggtcgggc tgagtctatt gttatttttc tttcttttga aaaaaatgag agcgaatctt
   124261 tttttattac tgatgtcagg gatgatttta ggtacgcttt ttggtagtgt cagtaccttt
   124321 ttacaagtca tcatggatcc gaatgaatat gatttgcttc aaggcagatt atttgcaagc
   124381 tttggcaata tccagagtca gtatcttttg cctgcttctt tgatcattat cggctgctgg
   124441 tacctgttat ttcgatatcg acatgtgcta gacgttttcc atctcggaaa tgaacaagca
   124501 gtaaacctcg ggatagacac agatcgtttt gaatttttga ttttatgttg tgtcagtctt
   124561 ttgacaggga catcaacggc tcttgtgggt ccagttactt ttttagggtt tattgtcgca
   124621 aatatcagtt atcgtgtctt ttctacttat cgtcatggtc agctatttat cggaagcttt
   124681 ctggtagcta tgctgtttct gacaggagga cagctcgcag tagagcagat tttcggctgg
   124741 aatacaacag tgagtgtcgt cgttgaattt gtcggaggaa tttattttgt cacaaaattg
   124801 attcgcgaaa ggaagagtat cctatgattg atttacaaaa ggtcaataaa ctgtaccatg
   124861 gaaaatcagt ggttaaagat gtacagttaa cgattcctga aaagaagttg actgctttta
   124921 tcggtccaaa tggtgctgga aagtcaacgg tgttgtcgat gatcagtcgt ttgataccaa
   124981 aagatacagg tgaaatctat ttagaccata acgaagtaaa ggcttggaaa tccaatgaat
   125041 tagccaaaaa attatcgatc ctacgacaat cgaatgaaat aaatgtccaa atcaccgtga
   125101 aagaattagt agaatttgga cgatttcctt atacgaaagg aaaattgacg acaaaagacg
   125161 aggaaatcgt tcaggaatca ctggaacaca ttgggttaga agaaatagcg gatcaatcga
   125221 ttgacactct ttcaggcggt caactccaac gtgcgtatat tgcaatggtg ctagcgcaag
   125281 acacggatta tattttactg gatgaacctt tgaacaatct agatatgaat tttgcggttc
   125341 aaatcatgca gattttaaaa aatttagtac aagaaatcgg gaaaacaatt gttattgttc
   125401 ttcatgatat caactttgct gcaagttatg cagacgaaat tgttgcaatg aaagatggac
   125461 agttatttac gcaaggaacg acccaagaaa tgataaaaaa agatgtattg gatgaattat
   125521 acgaaatgga catacggatt tgtgagattg aaggccgcag attctgtctt tatttctaaa
   125581 actaacatta ggagagaaga gatgaaaaaa ttagtagtag gtatcttgat tctagcatca
   125641 tttggtttag cagcatgtgg aaatagcaac aacaccagtc aggcagacac aaaaaaaagc
   125701 agcacacaaa ctgaaactac attgacgata acagatagta atggagacca aatcgaggtg
   125761 ccaaataatc cagaaaaagt cgtcgtattc gacaatggtt ctttagatac gatggatgct
   125821 cttggtgttg gagataaggt agttggagca gctacaagca gccttcctga gtatctttca
   125881 tcctataaaa aagtagaatc agcaggtgga ataaaagaac cagacttaga aaaaattaat
   125941 cagcttcagc cagatctaat catcatttct ggccgacaaa gagattttca aaaagatcta
   126001 agtcaaattg ctccgaccat tttcctttct ttagatgcga aaaatccatg ggaatctttc
   126061 cagcaaaatg tcactgcttt aggggagatc tttggtaagc aagaagaagc taaaactcaa
   126121 ttagaggaat tatctagtgc aatcgatcag acgaaaaaga aagcagaagc tactgacaaa
   126181 aaagcactag tcacacttgt gaatgaaggg caattatcag cctacggatc tggttctaga
   126241 ttcggtttca tccatgattt atttggcttt gaacaagcag atgatcaaat cgaagcatcc
   126301 actcacggtc aaagtgtttc ttatgaatat gtgctggaaa aaaatccgga tatcttattt
   126361 gtagtagaca gaacaaaagc tattggtgga gacgacagca aagatgatat ttctgccaat
   126421 gaactggtag cacaaacgaa tgctggtaaa aatcagcaga tcatctccct tgaaccagat
   126481 gtctggtatt taagcggggg cggtttagaa tcaatgaaac tgatgatcga agatgtgaat
   126541 caagcattta aatagcataa ataacagagg attaaactct catactatgt aacaataatg
   126601 tttcttacat tcttaaatag caagagccta tcctcaaaga agttatcaaa aaccatccgt
   126661 aaaatcgtat tgatgatttt acggatggtt tttatttatt tgagaaatct tttttcaacg
   126721 tgaattcatt atctacatca tacgggttga ttctagaatg gggagtggca aaaaatagtg
   126781 tgatccattt tttcagcatc aagatcgcct cctttgattt gaatcaaagg tacaaagcga
   126841 aacttagata aaacttaaat ttagatattt ttattttgta ccgatctctt ctttaaacga
   126901 tgttccatct gctgcttctc ggcaataagt ccactagact agaaaatatg agaagctatt
   126961 ttctgtccag cgttcttatt actcaaaaca aatcgcagtt gtcacaatct cttctaaacg
   127021 atgttccatc tgctgcttct cggcaataag cccactagac tagaaaatat gagaagctat
   127081 tttctgtcca gcgttcttat tactcaaagc aaatcgcagt tgtcacaatc tcttctaaac
   127141 aatgttcaat cagctgacct tcggtattag ctcaaagcac tgatttcaca acctctattt
   127201 aggcatgtgt ctcagcaagc ggttgatagt tgatgatctg catttttcga taaaaatcgg
   127261 cgggaagctt actccaccgt tttagtaatt gatagaaata actctcttct gctttgattt
   127321 tatactcgct taatatcaat ccaaaactat tcgttttttt tgaagctaat tttttataaa
   127381 tatccaatgc tttggtttta tcttcattga caaagagtgt ttttacttca atataagaac
   127441 cctcgatatt ttgtgtttct tcgataaaat agatgttttt tatatattcc ttcatagtga
   127501 ttcctccaaa gaagtctttt atttgataag tttactctac cgaagagttg ttaataatcc
   127561 gtgaacaaaa agtaattctt tttccaatag ttgttaaagg ttttttattt tttatagtat
   127621 tttttgttat ttaagaaagg aataaggtta aacaaataac aggttgcttt ttgggtcttt
   127681 ttccgttcaa gaaactgaac gaagaaaagg gaaagttgtc tcttttgtga taagagaaat
   127741 cataataaac gaatgactta ctttccaata aattgagaac agtcagaacc agacaaatta
   127801 gttgcatctg atgaataaag tgattattct atgacatgag gtgatacaga tgaaattaga
   127861 agtgacaaaa gaagcacaag aagtattgaa gaataaatta gaagcagacg atcaactttt
   127921 attagatatc gaaaacggtg acggtccatt tgcagaaagc caagtcagtt gccaattgga
   127981 tacggcattt cgattgatca ttgtcaaaaa agatacacaa acagatctaa gtctttattc
   128041 tgtagtggct gatacaccag ttggaccaat caaaatcaaa gacagtgcaa tactttacat
   128101 ggatgatcca tccacacttg ctttagaacc aacttataat agtttacaat taaaaggacc
   128161 aagcggcttg agaaaaggca acttgcaagt tgttcgaaaa gactagaggg ataaaatggg
   128221 aaaattggcg atcatcagcg acctacatgt ggacatcaat aaattcggcg aatcagagct
   128281 gatgcagctt tggcaagttt tgcaaaagca caagatcact cggcttcatc tagccggaga
   128341 tacggctaat aaagtagacc gttgcctagc tgttgtcgaa tttttcgtca ataaaggatt
   128401 atctacgaca tttcatttcg gcaatcacga gttggcagat gtgcccaatg aacagatgat
   128461 ggaccatttt ccagacccac attttttaaa tgaacggtat attgctttaa atgagcagac
   128521 cgttctttta gggatgaatg gctggtacga ctatcagttt tccgaattga aagattacga
   128581 ggcaatcgta cgaatgaagc gtttgtattg gtatgatcgg ttcatccagc gtgggaaaac
   128641 agatctcgag gtcaatgcag aaatgcttgc agctgcaaaa aatttgctag accagctgga
   128701 agcaaaaaaa cttgaggtga ttttatccac tcatttcgtt cctaaacggg catttatcat
   128761 gtatcaatct gctccttatg agcgttggaa caaattaaat gcatttttag gatcagaatc
   128821 attcggagaa ttacttgacc gttattcaaa tatcaaacaa gtagtttttg gccatacgca
   128881 tcgtcgcttt gaagataaat ccatcggtca cacgatttac agctgcagac cttttggtta
   128941 ttattatgaa tggcagctga cccgagattt tgtcttgaaa gaacagttgc tagacagcta
   129001 tgatcctatg aaattacgtg tcctcttgcg tgcccatcac ccagcttttc aggcctatca
   129061 aacacaacat ttgcaagagg aatttgaaca agcgatgaca atcgttccgt attgagacag
   129121 cattagtaat gtgtaaaaga aaataccttg taaaacataa ggataagtaa gagcagtaga
   129181 tccatttatc gtggatatgc tgcttttttt gcggaaaata atcagcgaat gtttatttga
   129241 tcggatgatt tgtaactcgc agagtactag atgcaagcgt gtattctttt tttgaacgat
   129301 aatcacaaaa aaaaatataa tatacatttt ttatttaatt ataattaata ttgattgaag
   129361 tctaggcagt caagtcgaga gtgtaaaata ttttgtgtaa atgaaaaaat ccatacaaaa
   129421 aaggaag
//
LOCUS       DACDIG010000002       118581 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.002, whole genome shotgun sequence.
ACCESSION   DACDIG010000002 DACDIG010000000
VERSION     DACDIG010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 118581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 118581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..118581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.002"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(27..1523)
                     /gene="lysS"
                     /locus_tag="IV247_000130"
     CDS             complement(27..1523)
                     /gene="lysS"
                     /locus_tag="IV247_000130"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAP8776232.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="IV247_000131"
     CDS             complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="IV247_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAP8776233.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="IV247_000132"
     CDS             complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="IV247_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAP8776234.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3743..5854)
                     /gene="hflB"
                     /locus_tag="IV247_000133"
     CDS             complement(3743..5854)
                     /gene="hflB"
                     /locus_tag="IV247_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAP8776235.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="IV247_000134"
     CDS             complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="IV247_000134"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAP8776236.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="IV247_000135"
     CDS             complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="IV247_000135"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAP8776237.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8054..8524)
                     /locus_tag="IV247_000136"
     CDS             complement(8054..8524)
                     /locus_tag="IV247_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAP8776238.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8570..8995)
                     /locus_tag="IV247_000137"
     CDS             complement(8570..8995)
                     /locus_tag="IV247_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAP8776239.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9062..9331)
                     /locus_tag="IV247_000138"
     CDS             complement(9062..9331)
                     /locus_tag="IV247_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAP8776240.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9342..10937)
                     /locus_tag="IV247_000139"
     CDS             complement(9342..10937)
                     /locus_tag="IV247_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8776241.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="IV247_000140"
     CDS             complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="IV247_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAP8776242.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14490..15050)
                     /locus_tag="IV247_000141"
     CDS             complement(14490..15050)
                     /locus_tag="IV247_000141"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAP8776243.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15403..16377
                     /locus_tag="IV247_000142"
     CDS             15403..16377
                     /locus_tag="IV247_000142"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP8776244.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16547..17947
                     /locus_tag="IV247_000143"
     CDS             16547..17947
                     /locus_tag="IV247_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAP8776245.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18025..18747)
                     /locus_tag="IV247_000144"
     CDS             complement(18025..18747)
                     /locus_tag="IV247_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP8776246.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18826..19332)
                     /locus_tag="IV247_000145"
     CDS             complement(18826..19332)
                     /locus_tag="IV247_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP8776247.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19455..21254
                     /locus_tag="IV247_000146"
     CDS             19455..21254
                     /locus_tag="IV247_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAP8776248.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21322..22896)
                     /locus_tag="IV247_000147"
     CDS             complement(21322..22896)
                     /locus_tag="IV247_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8776249.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23151..23660)
                     /gene="mreD"
                     /locus_tag="IV247_000148"
     CDS             complement(23151..23660)
                     /gene="mreD"
                     /locus_tag="IV247_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAP8776250.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23664..24518)
                     /gene="mreC"
                     /locus_tag="IV247_000149"
     CDS             complement(23664..24518)
                     /gene="mreC"
                     /locus_tag="IV247_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAP8776251.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24673..25311)
                     /locus_tag="IV247_000150"
     CDS             complement(24673..25311)
                     /locus_tag="IV247_000150"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAP8776252.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25312..25962)
                     /locus_tag="IV247_000151"
     CDS             complement(25312..25962)
                     /locus_tag="IV247_000151"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP8776253.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25975..26874)
                     /gene="rsgA"
                     /locus_tag="IV247_000152"
     CDS             complement(25975..26874)
                     /gene="rsgA"
                     /locus_tag="IV247_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAP8776254.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27039..29108)
                     /gene="pknB"
                     /locus_tag="IV247_000153"
     CDS             complement(27039..29108)
                     /gene="pknB"
                     /locus_tag="IV247_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAP8776255.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29105..29845)
                     /locus_tag="IV247_000154"
     CDS             complement(29105..29845)
                     /locus_tag="IV247_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAP8776256.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29858..31216)
                     /gene="rsmB"
                     /locus_tag="IV247_000155"
     CDS             complement(29858..31216)
                     /gene="rsmB"
                     /locus_tag="IV247_000155"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAP8776257.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31216..32154)
                     /locus_tag="IV247_000156"
     CDS             complement(31216..32154)
                     /locus_tag="IV247_000156"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAP8776258.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32147..32638)
                     /gene="def"
                     /locus_tag="IV247_000157"
     CDS             complement(32147..32638)
                     /gene="def"
                     /locus_tag="IV247_000157"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP8776259.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32653..35061)
                     /gene="priA"
                     /locus_tag="IV247_000158"
     CDS             complement(32653..35061)
                     /gene="priA"
                     /locus_tag="IV247_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAP8776260.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35314..35619)
                     /locus_tag="IV247_000159"
     CDS             complement(35314..35619)
                     /locus_tag="IV247_000159"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP8776261.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35624..36238)
                     /gene="gmk"
                     /locus_tag="IV247_000160"
     CDS             complement(35624..36238)
                     /gene="gmk"
                     /locus_tag="IV247_000160"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAP8776262.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(36725..37009)
                     /locus_tag="IV247_000161"
     CDS             complement(36725..37009)
                     /locus_tag="IV247_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8776263.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(37027..37917)
                     /locus_tag="IV247_000162"
     CDS             complement(37027..37917)
                     /locus_tag="IV247_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAP8776264.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(37937..38155)
                     /locus_tag="IV247_000163"
     CDS             complement(37937..38155)
                     /locus_tag="IV247_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8776265.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(38125..39825)
                     /locus_tag="IV247_000164"
     CDS             complement(38125..39825)
                     /locus_tag="IV247_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAP8776266.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            40092..40865
                     /locus_tag="IV247_000165"
     CDS             40092..40865
                     /locus_tag="IV247_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8776267.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(40946..41515)
                     /gene="thiT"
                     /locus_tag="IV247_000166"
     CDS             complement(40946..41515)
                     /gene="thiT"
                     /locus_tag="IV247_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAP8776268.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(41581..41683)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(41689..42660)
                     /locus_tag="IV247_000167"
     CDS             complement(41689..42660)
                     /locus_tag="IV247_000167"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP8776269.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(42754..44127)
                     /gene="glmU"
                     /locus_tag="IV247_000168"
     CDS             complement(42754..44127)
                     /gene="glmU"
                     /locus_tag="IV247_000168"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAP8776270.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(44630..45448)
                     /gene="purR"
                     /locus_tag="IV247_000169"
     CDS             complement(44630..45448)
                     /gene="purR"
                     /locus_tag="IV247_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAP8776271.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(45615..46439)
                     /locus_tag="IV247_000170"
     CDS             complement(45615..46439)
                     /locus_tag="IV247_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP8776272.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(46412..47092)
                     /locus_tag="IV247_000171"
     CDS             complement(46412..47092)
                     /locus_tag="IV247_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP8776273.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(47115..48053)
                     /locus_tag="IV247_000172"
     CDS             complement(47115..48053)
                     /locus_tag="IV247_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAP8776274.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(48298..49149)
                     /locus_tag="IV247_000173"
     CDS             complement(48298..49149)
                     /locus_tag="IV247_000173"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAP8776275.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(49303..51876)
                     /gene="mprF"
                     /locus_tag="IV247_000174"
     CDS             complement(49303..51876)
                     /gene="mprF"
                     /locus_tag="IV247_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP8776276.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            52017..52823
                     /gene="proC"
                     /locus_tag="IV247_000175"
     CDS             52017..52823
                     /gene="proC"
                     /locus_tag="IV247_000175"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAP8776277.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(52902..54458)
                     /gene="rny"
                     /locus_tag="IV247_000176"
     CDS             complement(52902..54458)
                     /gene="rny"
                     /locus_tag="IV247_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAP8776278.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(54869..55918)
                     /gene="recA"
                     /locus_tag="IV247_000177"
     CDS             complement(54869..55918)
                     /gene="recA"
                     /locus_tag="IV247_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAP8776279.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(56027..57268)
                     /locus_tag="IV247_000178"
     CDS             complement(56027..57268)
                     /locus_tag="IV247_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAP8776280.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(57508..58086)
                     /gene="pgsA"
                     /locus_tag="IV247_000179"
     CDS             complement(57508..58086)
                     /gene="pgsA"
                     /locus_tag="IV247_000179"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAP8776281.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(58295..59179)
                     /locus_tag="IV247_000180"
     CDS             complement(58295..59179)
                     /locus_tag="IV247_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8776282.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            complement(59957..61243)
                     /locus_tag="IV247_000181"
     CDS             complement(59957..61243)
                     /locus_tag="IV247_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP8776283.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(61236..62504)
                     /locus_tag="IV247_000182"
     CDS             complement(61236..62504)
                     /locus_tag="IV247_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP8776284.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            62756..63217
                     /gene="mscL"
                     /locus_tag="IV247_000183"
     CDS             62756..63217
                     /gene="mscL"
                     /locus_tag="IV247_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAP8776285.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(63276..63722)
                     /locus_tag="IV247_000184"
     CDS             complement(63276..63722)
                     /locus_tag="IV247_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8776286.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(63815..64060)
                     /locus_tag="IV247_000185"
     CDS             complement(63815..64060)
                     /locus_tag="IV247_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8776287.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(64175..64753)
                     /locus_tag="IV247_000186"
     CDS             complement(64175..64753)
                     /locus_tag="IV247_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP8776288.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(64830..65360)
                     /locus_tag="IV247_000187"
     CDS             complement(64830..65360)
                     /locus_tag="IV247_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAP8776289.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(65364..66224)
                     /gene="rlmB"
                     /locus_tag="IV247_000188"
     CDS             complement(65364..66224)
                     /gene="rlmB"
                     /locus_tag="IV247_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAP8776290.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(66217..66612)
                     /locus_tag="IV247_000189"
     CDS             complement(66217..66612)
                     /locus_tag="IV247_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP8776291.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(66609..68021)
                     /locus_tag="IV247_000190"
     CDS             complement(66609..68021)
                     /locus_tag="IV247_000190"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAP8776292.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(68018..68554)
                     /gene="cysE"
                     /locus_tag="IV247_000191"
     CDS             complement(68018..68554)
                     /gene="cysE"
                     /locus_tag="IV247_000191"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAP8776293.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(68876..70336)
                     /locus_tag="IV247_000192"
     CDS             complement(68876..70336)
                     /locus_tag="IV247_000192"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAP8776294.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPKMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(70506..71663)
                     /locus_tag="IV247_000193"
     CDS             complement(70506..71663)
                     /locus_tag="IV247_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAP8776295.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(71697..73067)
                     /gene="radA"
                     /locus_tag="IV247_000194"
     CDS             complement(71697..73067)
                     /gene="radA"
                     /locus_tag="IV247_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAP8776296.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(73130..73657)
                     /locus_tag="IV247_000195"
     CDS             complement(73130..73657)
                     /locus_tag="IV247_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAP8776297.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            73887..74693
                     /gene="proB"
                     /locus_tag="IV247_000196"
     CDS             73887..74693
                     /gene="proB"
                     /locus_tag="IV247_000196"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAP8776298.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            74690..75937
                     /locus_tag="IV247_000197"
     CDS             74690..75937
                     /locus_tag="IV247_000197"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAP8776299.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            76013..76357
                     /locus_tag="IV247_000198"
     CDS             76013..76357
                     /locus_tag="IV247_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8776300.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQRERW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(76474..76800)
                     /locus_tag="IV247_000199"
     CDS             complement(76474..76800)
                     /locus_tag="IV247_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8776301.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(77415..78617)
                     /locus_tag="IV247_000200"
     CDS             complement(77415..78617)
                     /locus_tag="IV247_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8776302.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            78734..79249
                     /locus_tag="IV247_000201"
     CDS             78734..79249
                     /locus_tag="IV247_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8776303.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(79448..79894)
                     /locus_tag="IV247_000202"
     CDS             complement(79448..79894)
                     /locus_tag="IV247_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP8776304.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(79941..81419)
                     /gene="msr"
                     /locus_tag="IV247_000203"
     CDS             complement(79941..81419)
                     /gene="msr"
                     /locus_tag="IV247_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP8776305.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(81773..82402)
                     /locus_tag="IV247_000204"
     CDS             complement(81773..82402)
                     /locus_tag="IV247_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP8776306.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(82386..82850)
                     /locus_tag="IV247_000205"
     CDS             complement(82386..82850)
                     /locus_tag="IV247_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8776307.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(83337..84605)
                     /locus_tag="IV247_000206"
     CDS             complement(83337..84605)
                     /locus_tag="IV247_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAP8776308.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(84836..85573)
                     /locus_tag="IV247_000207"
     CDS             complement(84836..85573)
                     /locus_tag="IV247_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8776309.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(85640..86208)
                     /locus_tag="IV247_000208"
                     /pseudo
     CDS             complement(85640..86208)
                     /locus_tag="IV247_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
     gene            86336..87208
                     /locus_tag="IV247_000209"
     CDS             86336..87208
                     /locus_tag="IV247_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8776310.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            87550..88422
                     /locus_tag="IV247_000210"
     CDS             87550..88422
                     /locus_tag="IV247_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8776311.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            88419..90377
                     /locus_tag="IV247_000211"
     CDS             88419..90377
                     /locus_tag="IV247_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8776312.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(90709..91560)
                     /locus_tag="IV247_000212"
     CDS             complement(90709..91560)
                     /locus_tag="IV247_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8776313.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            91683..92150
                     /locus_tag="IV247_000213"
     CDS             91683..92150
                     /locus_tag="IV247_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAP8776314.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(92413..92895)
                     /locus_tag="IV247_000214"
     CDS             complement(92413..92895)
                     /locus_tag="IV247_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8776315.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(92995..93858)
                     /locus_tag="IV247_000215"
     CDS             complement(92995..93858)
                     /locus_tag="IV247_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8776316.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            94456..95331
                     /locus_tag="IV247_000216"
     CDS             94456..95331
                     /locus_tag="IV247_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8776317.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(95476..95628)
                     /locus_tag="IV247_000217"
                     /pseudo
     CDS             complement(95476..95628)
                     /locus_tag="IV247_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(95896..97227)
                     /locus_tag="IV247_000218"
     CDS             complement(95896..97227)
                     /locus_tag="IV247_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8776318.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(97376..98089)
                     /locus_tag="IV247_000219"
     CDS             complement(97376..98089)
                     /locus_tag="IV247_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8776319.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            98264..99688
                     /locus_tag="IV247_000220"
     CDS             98264..99688
                     /locus_tag="IV247_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8776320.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            99987..100694
                     /locus_tag="IV247_000221"
     CDS             99987..100694
                     /locus_tag="IV247_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAP8776321.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            100681..101004
                     /locus_tag="IV247_000222"
     CDS             100681..101004
                     /locus_tag="IV247_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP8776322.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(101093..102130)
                     /locus_tag="IV247_000223"
     CDS             complement(101093..102130)
                     /locus_tag="IV247_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8776323.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(102451..103434)
                     /locus_tag="IV247_000224"
     CDS             complement(102451..103434)
                     /locus_tag="IV247_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAP8776324.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(103849..104619)
                     /locus_tag="IV247_000225"
     CDS             complement(103849..104619)
                     /locus_tag="IV247_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8776325.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMIFGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(104612..105547)
                     /locus_tag="IV247_000226"
     CDS             complement(104612..105547)
                     /locus_tag="IV247_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8776326.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(105863..107512)
                     /locus_tag="IV247_000227"
     CDS             complement(105863..107512)
                     /locus_tag="IV247_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP8776327.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(107629..110232)
                     /locus_tag="IV247_000228"
     CDS             complement(107629..110232)
                     /locus_tag="IV247_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP8776328.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(110244..111293)
                     /locus_tag="IV247_000229"
     CDS             complement(110244..111293)
                     /locus_tag="IV247_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAP8776329.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(111319..111633)
                     /locus_tag="IV247_000230"
     CDS             complement(111319..111633)
                     /locus_tag="IV247_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAP8776330.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(111649..112095)
                     /locus_tag="IV247_000231"
     CDS             complement(111649..112095)
                     /locus_tag="IV247_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP8776331.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(112096..114072)
                     /locus_tag="IV247_000232"
     CDS             complement(112096..114072)
                     /locus_tag="IV247_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAP8776332.1"
                     /translation="MKIEIIIVHLSCIQEVKIMGIEFSERQMKILYFLIENEHTTYKE
                     LSEQLYVSTKTISKDILILKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKIN
                     IADSPEDRLTYVFAKLLNTNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLV
                     SDRNGVRIKADEKSKRRLMSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDI
                     LKKVAELLNRYMNKTQLKFTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNT
                     LILIKMIEKAFSIIVPIDEQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVR
                     IKLNEFYPDEELINNLVLHLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEE
                     IEERFCIHFNEDEIAYVTLHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFA
                     DMVEIEDVLSINELQDRKVTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQL
                     NEQTEDAFLNLLDKRFCFFTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKL
                     SSTAMNDFAMPHIEVDFIKKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKI
                     YETFNEIASNHELLANLKTSSSFEKLVYILKEGL"
     gene            complement(114230..115126)
                     /locus_tag="IV247_000233"
     CDS             complement(114230..115126)
                     /locus_tag="IV247_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8776333.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(115119..115799)
                     /locus_tag="IV247_000234"
     CDS             complement(115119..115799)
                     /locus_tag="IV247_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8776334.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(115800..116582)
                     /locus_tag="IV247_000235"
     CDS             complement(115800..116582)
                     /locus_tag="IV247_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8776335.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(116575..117492)
                     /locus_tag="IV247_000236"
     CDS             complement(116575..117492)
                     /locus_tag="IV247_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8776336.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(117656..117979)
                     /locus_tag="IV247_000237"
     CDS             complement(117656..117979)
                     /locus_tag="IV247_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8776337.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
BASE COUNT        33718 a        25226 c        20936 g        38701 t
ORIGIN      
        1 gaattcctta gacagctttt attcgattat ctcattgttg ggaatagcaa aacatcccga
       61 attgattgtg catcagttag caacattact aatctgtcga tcccgatgcc tagaccacca
      121 gttggcggca taccgtattc taaggcttcc aagaagtctt cgtctacacc gtgagcttcg
      181 tcatttccta gttcacgttc tttttcttgc gcttcgaatc tttctctttg atcaattgga
      241 tcgttcagtt ctgtaaaggc attagcaaat tcgcgcccca ctatgaatag ttcgaatcga
      301 tctgtgaatc gaggatcttc tggatttttc ttcgctaaag gcgaaacttc gactggatgt
      361 ccgtatacaa acgtcggttg tgtcaatgtt tcttcaacaa atgtttcaaa gaattcattg
      421 ataacatgtc cgacacccat agcttcggtt acttctacat tgtgtttttc ggctaaagct
      481 aatgcttctt ccaatgtcat ttcttgccag aagtctacac cagtttgttc tttgattgca
      541 tctaccatat ggatgcgttt aaagtcactt tctaagtcga cagcttgtcc atcatatgtg
      601 atttttgctg ttcctagtac tttttcagct gcgttacgga taataccttc tgtcagattc
      661 attacatcat tgaaatcagt atatgctgtg tatgcttcta acatcgtaaa ttctggatta
      721 tgtgtagtat caattccttc attacggaaa acgcggccga tttcataaac tttttccatt
      781 ccgccaacaa ttagtcgttt taaatgaagt tctagcgcaa tacgcaaata taaatcaatg
      841 tccagtgcat tatgatgcgt gataaatggg cgtgcggcag ctccacctgc ctcattgtgc
      901 aatactggtg tttctacttc aatatagcca ttgccatcta aatagcgacg gatttcgctg
      961 ataatttggc ttcgtttcat aaatcggtca aagctttcgc gattactgat taaatccaag
     1021 tagcgttgtc tgtagcgttg ttcaatattc gttaagccat ggtatttatc tggcagtgga
     1081 cgaagtgctt ttgacaaaat cgtgatttct gaagctttga tagaaacttc gcctgtgtct
     1141 gttttcatga tttgtccagt tacaccaaag aaatctccaa ggtctgcatg tttgaatact
     1201 tcataggcct catcacctac ttggtctttt cttacataaa tttgaatttg gccttcacga
     1261 tcttgtagat gcgcaaatcc agttttccct tttcctcggc gagtcatcat acgaccagct
     1321 acactagcgg tcaatcccat ttctgctagt tcttcttttg ttcttggatc aaatagttca
     1381 tgcaattctt ctgagttgtg cgtgcgttca aatcgttttc caaatggatc gattccatct
     1441 tcacgcaatt gttccatttt ttgacgacgt acaagcattt gatcatttaa atcttctact
     1501 tgatgttgtt gttcctgtgt cacaaaagtt cctccattct catgttaatc tctttcctat
     1561 aatgccaatg aaacacaaaa aaagcaagcc gaacttggaa tttcggcttg aaaattcacg
     1621 aaaaacttag aaatcacaac gtttgtttca atagtacttt ttccgctttt tctacaaatt
     1681 gatccagcag gtcattgatt tcttgctgtt tttctgtctg attgattgct actttcactt
     1741 tcgctgctcg aggaatccct tttaaataat aagccgcatg tgttctgaat tctctacaag
     1801 ctactttttc tcctttgaga tctgctaagc gttgtaaatg aagtttagct gtagcaattt
     1861 tttcggctgg tgttggttca ggaatcagtt ctcctgtttc gagataatgc tgtgtgcggt
     1921 gaatcatcca tggattgccc agcgctgcac gaccaatcat cacaccgtct gcaccgacat
     1981 agtcaagcat ccattttgca tcttctggcg ttttgacatc cccgttcccc ataaatggga
     2041 tcgtcaaatg ttttttcact tctttcaata cttcccagtc cgcttttcct tcatacatct
     2101 gaacccgtgt acgtccatgc atagcgatgg cacttgctcc agctgattgt gctgctaatg
     2161 cattttctac agcaaagacg tgctcttcat cccaaccaat acgcatcttg attgttactg
     2221 ggatatttac tgctgaagaa acagcttgga ccatttcgta tactttctca ggatctaaaa
     2281 gccattttgc tcctgcttct gctttgatta ctttatttac tggacagccc atattgatat
     2341 ctattatatc ggcagctgtg ttttcttcga caaattgagc agcttcaact agactatctt
     2401 tatttccgcc aaatatctgt aaacttaatg gatgttctgt ttcttcaatg tggagcatct
     2461 ctaaagtttt tttgtttctg aaatgaatac cttggtcact gatcatttca catacgacta
     2521 agcccgcacc aaattctttg acagtgactc gaaatgctga atttgttatt ccagccatcg
     2581 gtgcaacaac gacacgatta gggatttcca cattgccaat tttccatgtg tgttccactt
     2641 aaatccctcc taaggcttct ttcttcaatc catctaattc ttcttcgcta tacttgtatt
     2701 tgttattaca gaaagaacaa accgcttctg caccgtgatc ttcgtctatc attgcttgga
     2761 tttcttctgc acctaacgcg ataattgctt ctgcaaattt ttcttttgaa cagtcacatt
     2821 tgaattgaac aggcattttt tccaaaatgt tcaattcttc tgtaccaagc aatcctttca
     2881 atatttcttc tggtgtctgt ccttcatcta agagggtaga gattcttggt gtcgttttta
     2941 attgttcttc gatcttgctg attgtttctt catcggcacc tggcatgatt tggatcatga
     3001 atcctcccgc cgtcttgatg ctgtcgtcag tattgaccaa tacactcagt ccaacagctg
     3061 aagggatttg ttctgagata gcaagatagt aagtaaaatc ttcagcgatt tctccagaaa
     3121 caatcggcgt ttgcccagaa aaaggttctt ttaatcctaa gtcttttgtg acagtaaaca
     3181 ttccttgtgt tccaactgct ccccgcacat cgattttacc ttcactattt agaggaagac
     3241 tgagatgcgg atttttgatg taacctttca catctccctt tccatttgca tctactacaa
     3301 ttcctgttgc tggaccatcg ccttggattc gaacagttag tttgtcttct cctttgagcg
     3361 tcgcaccaag tagcagacta ccgaccaatg tccgtcccaa agcagctgag gaggtgtgcc
     3421 aagtatcatg acgtctttgt gcctccgcta cggtatttgt tgcttgaacg gcgtatgctc
     3481 ggacgaatcc gtcgtgagca agtgccttaa ccaaataatc tttcatttgt tatgtactcc
     3541 tttttccgtt tttaaactca tagtgaatca tactagcaac tactgctttt ttcaactttg
     3601 gaatgcttct tttaaaaata attcagaatc atctagaaaa caggacatca ccctatttac
     3661 aacttaaata aagaaatctc ccgaaaaccg gtttttgttc cggttttcgg gagtcatatc
     3721 agatcaattg acctatattt ttttatttgt attgatcgtc tgaatgatta tcttctgatt
     3781 gttgagaatc tgaatcatgt tctgctttat tttcctcatt cagttcatgt tttgcttctt
     3841 caaaatcacg tttttcttcc gcttgttttt cagcgtcttt ttcttccaaa gcacgttttg
     3901 cttcttcaaa agttttcgct tctttttcgc ttggataatc atttgactca gtaccttcag
     3961 gcatcacacc gtgttcaaac aatgacttga ttgctttggc atccaatgtt tcgaattcca
     4021 aaagtttttc agcaatcagt ttatgctgtg cacgatgagc ttcgatgatt tctcgagctt
     4081 tatcatgagc ttccatcaag atttttcgta cttcttgatc aatctcgaaa gcgacttgtt
     4141 ccgagtaagc ttttgtttga ccatagtcgc ggccaacaaa cacttgatgg tttccttcat
     4201 attgaacagg acctaaacga tcactcattc catattctgt taccatgctt cgtgctagag
     4261 ctgtcgcttg ctcaaagtca ttggaagcac cagttgattg gacattgaag atgatttctt
     4321 cagctgtacg tccaccaagt aagcctacga tttgttcaaa catatcttct ttcgtcatca
     4381 agaactgatc ttcttttggt aaagcgatca tatatccgcc ggcgcgtccg cgagggatga
     4441 tcgttacttt gtggacgacc cgagcgcgac tcaatactaa tccgacaatc gtatgaccag
     4501 cttcgtgata agcaaccatt tcgcgttctt ttttgctgat tacgcgatct tttttagctg
     4561 gacctgcaat cacgcgatct tccgcttcat cgatatcaga agcatcgatt ttctttttat
     4621 ttcttctagc agctactaac gctgcttcat tcaagacatt ttctaaatct gctccggcaa
     4681 aacctggtgt ctgttgagcc acaactttca agtcaacgtc atctgccatc ggtttattcc
     4741 gtgcatggac tcgcaagatt gcttcacgac cttttacatc tggacgtcca actaaaatct
     4801 ggcggtcaaa acggcctgga cgtagtaacg ctgggtctag tacatcagaa cggttggttg
     4861 cagcaatgac gattactcct tcatttccgt cgaagccatc catttctaca agcaattggt
     4921 tcaatgtttg ttcgcgttca tcatgtccgc cgcccatacc ggcaccacgt tgacgaccta
     4981 ccgcatcaat ttcatcaatg aaaatgatag ctggtgcgtt tttcttcgct gtttcaaaca
     5041 aatcacgaac acgacttgct ccaacaccga caaacatttc tacgaaatca gaacccgaga
     5101 tcgagtaaaa cggtactcca gcttctccgg caacagcttt tgctaataag gttttacctg
     5161 ttcctggagg tccttctaaa agtacacctg ccgggatacg ggcacctaat tcaacaaatc
     5221 ggcgaggatc tttcagaaat tcaacaactt caactagttc ttgtttttct tcttccgcac
     5281 ccgctacatc agaaaaacgt acgcgattag cttttttgtc tgcttctttg gcttttgatt
     5341 taccaaagtt catgacacgg ccgccaccgc caccgccgcc gccttgttgt cccatcatca
     5401 tgtaaaagaa aaagatgatg attacgattg gcaagaagct aagcaatagg gaaacccata
     5461 taccgctgct tgattcttct tttactgttg tagcaacttt gttttctttt gccagttctg
     5521 ttacttggct caatgttgta tcatttggca aaatgattgt tgtgaaatgg gtagtagtcg
     5581 tatctgttga acctaagata gctaacccac cgctgttagc gacggtctgc tgttctttgt
     5641 attcaccagt gatcttatat acaccatttg caggctgcac ttcaaattct ttgatcttgc
     5701 ctgcttgtag ttgttctgtg aattttgagt actcgatatc tgacgatcgc tggccgttat
     5761 taccaaaaat gaaataaacg accattacca tcgctaagat tacgaggaca taatacaagg
     5821 catttttcat catgccgttg ttctttttgt tcatagttgc cctccctaag cgtcaattta
     5881 atttaacagt tttttatcaa tcctgaccat tctattgtac cataatttaa ttgagataca
     5941 gcattaattt gattgataaa cactaggttt caatacaccg atgtacggaa ggttacgata
     6001 tgcttctgca taatccaagc cataaccgac cacaaattca tttggtacgt tgaagccaac
     6061 ataatctgct tcgatattta ctactcttcc ttcaggtttg tcaagtagtg taacaatttt
     6121 gactgatttt gctttgcggt atttgaaaag atccactaaa taagcaagtg ttcttccgct
     6181 gtcaatgata tcttcaacga tcaacagatc gcgaccttct acattcgtat ctaagtcttt
     6241 gacgattttt acttcccctg acgagactgt tgcattcccg tagcttgaaa catccatgaa
     6301 atccaattcc aaatacgtat cgatagaacg aacgatatct gccatgaatg gtaccgctcc
     6361 ttttaagaca cctactacta ggggattggt gttttggtaa atttctgtta actctttgcc
     6421 tagttctttg caacgaactt gtatctcttc ttgtgagatc aaaattctct caatatcctt
     6481 ttccaacatc aggttctcct tccaattgct tctttttgat atttgtaaag aagtatgtag
     6541 tgtattttat cagtttcttc acgaatactc aaatatgaaa aggcaaaagg tatcaagcta
     6601 tatatatttc ttttggcatc tgtaatgatc caagcatgct gcctttgtga attaggtact
     6661 ttctgatcga taaaatatct agatactttt tttgtgagat tctctgttaa tttaatgcgg
     6721 tctcctgctt ttcttttttc gacatacagt tgcacatcag caggaatcca aatgtcatga
     6781 gaaaatagag accatgaatc tgttgctttg atgtcccctg cattttcagg tttgaccaga
     6841 ccaatccatt ctgtttcatt caaatatatt ccttgttctg gaatcagcaa caagcgctgg
     6901 tcttctgtct ggctagttgc ttgtttttct tttttagaga gtacaacaaa atgatactct
     6961 cttttcagta tccaattatt gccaatactg agttgccatt ggcttttttt ctgttgccac
     7021 tggtctaaaa tcgactccat ttgttgttct ttcaaaataa ttcctgtttt tgagaatgct
     7081 ttgtcgaaaa aggagtaaaa aaaatagtat cgttcagcat ctgtcatttc ttcaatcatt
     7141 tctgcagaaa atcgaaattg ttcctcccac tgttcgactt ccttttcgat caactgtccc
     7201 atatattttt gaatgacttg gtctgcccat tggatctgtt gcgaaaattg ctggaaatgt
     7261 ctcaacgcct gtgtattttc ttgtttcaat tgaggaacta ccaaatgacg aagccgattt
     7321 ctctggatat ctaacaattg gtttgtctga tcctcaaagt aaacaagctg cgattccgct
     7381 gcataagtat ataggtcttc tttgctaaaa ctcagcaacg gacgaatcag tctgcccgta
     7441 gcaaatggtt gtacttcttt gattccagaa aaagtattta actgtccccc acgaacgatt
     7501 ttcattaaca ccgtctcgat ttgatcatct ccgtgatgag cagtcatcaa cgtgtcatat
     7561 tgtccttctt ccatcacgcg agtgaattct tgataacgaa agttacgagc ttctgtttct
     7621 atggcggatt tggctggatc ttcccaaata cttacatata aagggagatt cttctcttca
     7681 caatatgagc gaagataggc agcttcttct gctgattctt ctcttaattg gtgattgatg
     7741 tggacgatac ctaaagaaaa accgatttgg cgttgcactt gtaaaagtaa atggagcaaa
     7801 accatcgaat caacaccacc tgataccgct gctaaaattc tcgatccttg ttgccagaat
     7861 ccttgttcat tcccgtgccg aataaatgac tgctgtaata gcggtgcttt tttcccgcct
     7921 ttttcaacgt tcatcctttc cacacccttt cttcctaaaa aaaagagagc gggaaaacgt
     7981 ctaaaacgac aattaacctg ccctctgttg atactatgac ccaaatagtc gcactatcat
     8041 tatttgcggc tttttagctg cgacgtccgc cacggccacc gcgctttcct tctgtgtttc
     8101 gcttcaatga agataagcgg tcatcgctat ctttcaaaaa tgaactcatc aatgaatcga
     8161 agtcttcttt ttgagctgaa ggttgactgc gagaaaaaga ttttttctgt gctggacgat
     8221 ttcggttgtc acggggatct ttttgttgat attttttagg acggctttct tctccagatt
     8281 cagcaggttg ttcctgcgct ttccggatag atagtccgat ttttccatca tcatttactg
     8341 aaataacttt aactgttact tcatctccta cacttaaaac atcatggatg tcttttacat
     8401 agccatttga cacttcgcta atatgaacta gacccgtttt tccagcacct agatcaataa
     8461 acgcaccaaa atttgtgatt cctgatactt ttccttgcag ttttgctcct acctcgattg
     8521 acataaaaaa aatgttcctc ctaattattt ccctttttta tattcctgct tattttgcag
     8581 ttgtagaact agttgattga tctgtttcat ttgtcgagct gtctgtattt gattctaata
     8641 taggataaac tttttcaccg tcttttgagt aaaagaattt gcttctggca agtttagcta
     8701 catactcatc atctttcagc aatgaaacat cttggtgcag ctgatcaacg tctgcgttga
     8761 cttctttcat ttcagtattt gcttcgactc tcaattcgtt caattgatgc atcctttgca
     8821 tgtcatggaa aatctgcaca cctattatcg cgaaaatgac caaagcacca acaaaaattg
     8881 cagccagtct gcgtcttcga aaaatcagtt gacgttgttg ttgctgaaat tcagcatact
     8941 ttttctttgc atattccgta tctagcgcag cgattttatt gctttttttc gtcacttgga
     9001 ttcgctccaa tctttttgat tgtctttttc attataccaa tatcgtgaaa gcttgtctaa
     9061 tctatttctc tagtgtttct gtgcgatttt cagaaataat ttcgtacatt ttttgtgcat
     9121 cttctttttt ggttgattca tgtaattccg taattttcac ttccaacgtt ttgttgccaa
     9181 actggacttt caaaatatcg cctactttga catcactgga agatttcgcc aatttaccgt
     9241 tgatttgaat tcttccttta tcagctactt cttttgctac tgatcgtctt ttgatgattc
     9301 gggaaatttt taaaaattta tctaatcgca tatcttattc ctcacttttt aattttttta
     9361 tttaatcgca ggattttctt accaaaaggc aacattagcc attctcgtac tgtaaacaga
     9421 cgaagacgaa tcactgcata aatgaacaca gaagccccga tcccaactcc tatcacgctg
     9481 acaaaaaaag cagtggaacg atgagttaca ggaccaaata ctatattcaa tacaccataa
     9541 tacagcagca gtactactgt catacaaaga aggctgaaca tcaacttccg tccaaaatgc
     9601 cgttctttcc aaaaagcatt tagctctttt gtttcaattc gaacaaaata ccaaagcgtc
     9661 acacctaatc ctaaaagggt agacaggctt gcacctgctg ttcctaataa accggtcaga
     9721 aaagaagtag tcaatccttt taccaatagt ccccatcctg cgccttttaa agaagggcga
     9781 aacgagttct tgctttgagc aatactttga taagcttgga tgactgcagt gaaagcaatg
     9841 gaaaaaacaa ataatacaag cgctgcattt cccgcataat ctttaaacaa cgcataattg
     9901 atataaggga gcagcaaggc taatcccaaa gacgcagcta atgctaaagc ggtcgtcaat
     9961 ctaagataga ttttggctgt ctgtaaaaac tgacgataga tccggttggt caaatgacga
    10021 gtcaacgcag gcaggaatgt cgcacttaat gcagtggcaa tcactaaacc taattgtact
    10081 aaaggctgac ctcgatcata gacccctttt gctatcttcg caccgtattc tgacagtccg
    10141 taaacttcca aagcattttt tacaaagaaa gagtcgatca gctggaaaaa gataagcaat
    10201 ccactataaa tcgaaaccag tccgccttct accagaaaac gccggatcaa gggctttgac
    10261 ggctgtttga acaatggaaa ccgtcggaag cttaacgctc ctccatgaat cttttgttca
    10321 tagtagtgaa ggatcccata tgcgcataaa ccgccaaaaa cagctccact cattgctgcc
    10381 gtccctgttt ggtacaccgt ccatccaagc tttctaaaag caaacgcact acagacaatc
    10441 acaaaaacac gaacgaactg ctcaactact tgagaaacag cagttggttc catcaagaat
    10501 cttccttgga aattccctcg ataaaaagat agaccaggca tcagcaaaaa ggtaaaggaa
    10561 actacttgga tcagcggttt caattgtatg tctcccatca tccaagcaat ccagctgcta
    10621 aatgcaaaga caaacaacca caaaaagaca cctgtccaaa atactaaggg ataaagattt
    10681 tgcaaagctt gctttcgctc gaaaggattc tttctttctg caacgtactt tgatatgaac
    10741 tgcggaagac cggataaagc taaagtcatt gctatcccat aaattggata aacttgctga
    10801 taaacataaa aaccttcgtc gccgactaag ttttggaggg gaattcggta aagggcactt
    10861 aatacttttg ctatgaaaga agcaatcgtc aatacaaatg ctccttgcat gacgcgccgc
    10921 atttctttat gatccaaatc attgctcctt cctacgctga cagatatttt tgctcacgca
    10981 acgctttgac aaacttttgg atttcctgaa gccatactgc ttcttccatt tggttgggga
    11041 tcgtcaagcg tatcaccatc tgttcttttt caacgcctag actggctttt agttttgtcg
    11101 ctgtcagtgc ttcgaataac tgttccacac tatagcgttt tgttccaact ttacttagag
    11161 taaagactat atgcgcctcc tgtttcctta tcgtttctag gagcgctcga tcgccatcca
    11221 ttttgatctg cccaattgtc aagagatgtg ctacttcttc tggatattct ccaaaacggt
    11281 cgagcagatc atcttctagt tcatctagca tttcttgcga atcaagttca cgaattcgtt
    11341 tatagatctc gattttctgc cgttgatctg ccacgtatgt ttcaggtaga tacgcatcga
    11401 caccgaggtc gatctcaaca gtggtcttct ccacttgact attctttcct tgtttacgag
    11461 aaacagcttc gcttagcatt tgagaataca tatcgaaacc gactgcatca ataaatccat
    11521 gctgctgagc acctaagaga ttacctgcac cgcgaatgga aagatcccgc atcgcaatct
    11581 tgaatccaga tcccagttct gtaaaatctt tgattgcctg aagacgtttt tcactgactt
    11641 cgttaaggat cttctgctgc tcatacataa agtaagcata tgcgacacgg ctgcttcttc
    11701 ctacccgccc tcgcaattgg tataacgtgg ataatcccat ataatcagcg ttctcaacga
    11761 acaacgtatt tgcgttcggg atatctactc ctgtttcaat gatggttgta gtgacaagaa
    11821 catcatattg accttcaata aattcaaaaa gcgtattttc taattgcgcc tctgtcattt
    11881 gcccatgagc ataaccgatc cgtgcttccg gaacgagttc ttggatttct tctacttttt
    11941 gttcgatcgt ttctacccga ttatataaat agaatacttg tccacctcgt cccatttctc
    12001 gatggattgc ttcgcgaata gccccaggat ttttttccat aacatacgtt tggatcggat
    12061 aacgattcgc aggcggtgtt tcgattactg aaagatcccg cacgcctaac atcgacatat
    12121 gcaaagttct cggaatcggt gttgcagtca gagtcaaaac atccacttgt gcacgtaact
    12181 gtttcaatcg ttccttatgt tttacaccaa aacgttgttc ttcgtcaatg atcagtagac
    12241 ccaaatcgct aaattcaata tcttttgaca aaattcggtg ggttcctaca acaatatcaa
    12301 cttgacctgt acgcagttgt tcgatggttt ctttttgttg ctttcttgtt ctgaaacgac
    12361 ttaatagccc gatatttacg gggaaacctt caaatctttc cagcatcgtt tcatagtgct
    12421 gctgtgccaa gatcgttgtt ggaactaaaa aggctacttg tttgctttcc tttatcgctt
    12481 taaaggccgc tcgaagcgct acttctgttt tcccataacc aacatcacct acgagtagtc
    12541 gatccattgg tttttctttt tccatatccc gtttgatctc cgctgcactt ctcaactggt
    12601 catcagtttc agagtagggg aaggcatttt cgaattcttt ttggtaggcg tcatcaggtc
    12661 caaaagcata tcctttttct gactctcttg cggcatataa tttgattaga tcgtcagcga
    12721 tatcctcgat ttttgaagag acttttcgct tggttttcgt ccattcactt ccgcctagct
    12781 tattgatgcg aggcgctttg gcttcagagg cgacatattt ttggatcaga ttcagttgag
    12841 taacaggaat aaacagtttg tcgtcgtttt gatacaaaat agtcatatag tcttggtgaa
    12901 cgccatctac ttctagcgtt tccatcccaa tgtatttccc gataccatga ttggcatgga
    12961 caacgtaatc tcctgctttt aactcattat agcttttcaa tcgctctgcg tttgttactg
    13021 tttgacgtct cgtttgtttt ttcgtcgttt tttggaaaat ttctttttct gtgatagcta
    13081 ctattttttc ttgaggaagt tcaaaaccag cctgcaaagc tccttcaact aattgtacct
    13141 gtcctctgat caatgcattt ttttctgttt ctactactaa tatgtcttca tctcgaagca
    13201 tttgttctgc tttgcgtatt ctgtcctttg tcggaataaa gatcacaaca gtctgttttt
    13261 gcttgcgcca acggtccatt tctgttttca gcaatggcat ttgtccgaag aactgttgca
    13321 tcgtccgata ttgaaaatta tggattgctt ggaaacgaag atttcccatc cctttttgga
    13381 agagtgagaa aaaggttgtg gtgaactttt ccttctgcac gattttttgt atatctgctg
    13441 aaaatgtctg ctcagaaaac acccgcaatt cgctgatttt ttgagtctgc cattctgccg
    13501 cttccctttc gatttcccga tttgtttcca tgattcgctg ataatcatca acgactagta
    13561 aactatttga agggaaataa tccaagatcg tcacagattc ttggtaaaga agatctgagt
    13621 agaattttgc ttcttctgtc gggacacctg cttgccagct tgaagtcagc tgaccgaagt
    13681 aatcttgcaa aaactcgcgg tctgcttctt ctgtggtcgc agcaagccgt ttttctagca
    13741 ttttttgcat ttgctctatt ccatgttcta gatcctctgg tccgtagact tgttcactcg
    13801 ttggggaaac ccagatttct tccgtttttt cgattgagcg ttgtgtttct gcttcaaaat
    13861 accgcataga atctatctct acatcaaata gttctacacg aacaggatag gttgtcgtta
    13921 acgggtaaat atcgataatg cttcctcgaa tactgaattc tcccggtttt gcgaccatct
    13981 cttctcgtac atagcccatc aaagtcaatt ggcgaggaag attttccacg tctatctcat
    14041 caccaatatg ccaattcaat tgcgcctgta gccaagtttt cttattcggc agtcgtttgt
    14101 gcaaagatgc gactggcaac aaatagatac ctgctttttt ttcttgaagt gcgttaagcg
    14161 ttgcaactct ctcgcctcgc gcttctggag aagaaaaggc catttcagca gaaagcactt
    14221 catccactgg aaataaatgg atctgatctt gcggaatcac atgttgcaga tcttctgcta
    14281 actgattcgc ataatacaaa tttggcatca gaataatcat gtttttattt gttttcttaa
    14341 acgctcgagc aaatagaagt gttttcgctg agcccgctaa tcctgtgatt agttgtctag
    14401 gcattttttc ttctaaattg ctgacccatt cttttacttg cggatctttt ccgactaatt
    14461 gaataatatc cattcctttt cctccttcct caattgaatc gattcatcgc atccacgaac
    14521 gaatgccctt ctatgagata ttcgactgct ttaacagatt gacacatgct ttcttctatc
    14581 aaaggctgat tttctttgct gaaaggacta agcacatgct ggactactgt ttttttccct
    14641 tctggcctgc cgattcccac cttgattcga tcaaagacat tcgtattcaa atgggaaata
    14701 atacttttga taccgttatg cccgccggca cttccctttt gacggagccg gatttttccc
    14761 actgccagat ccaaatcatc gtagattacg actaattcct ctggataaat cccaaaataa
    14821 gtcattagtg ggccgactgc acgaccagat tcattcatga atgtttgtgg tttgactaaa
    14881 aggatctttt ctccattatg aaaaaattcg gcaacttctg cttcgaacgg attttttttg
    14941 aaagtagctt gatgctcgcg tgcaatttta tccacgacca taaagccgat attgtgtttt
    15001 gtatattggt atttggtacc tgggtttcct aacccgacga tcattttcat ttttgttttc
    15061 cacctaattc taaaaagcag cacaaaaggc tggactgcta attagcggtc cagtccatcg
    15121 agcttttttc ttttttatac ttgtcaatgc atagtataac acaaaacaag gcgtttcggc
    15181 taaaagagaa tcgttaataa atgataaaga aattaaaaat ttcttcaaag tttaatgaaa
    15241 taattgatat ataaaaatag tgaaataacg cacatgtaag cttttacata taatacaacc
    15301 ttgttatctg ttatatttta tagccaaata gcgtgacatg gggcttgaga aatgttagca
    15361 taagcatggg aaaaaacaaa tcaatggaag gattgatact aaatgactgc agctgcagaa
    15421 aaaaaagatc atcaaaaagt tattcttgtt ggagacggcg ccgtaggttc tagctatgcc
    15481 tttgctctag ttactcaaaa tatcgctcaa gaagtaggta ttgtggatat caatactgct
    15541 aaaactgaag gagacgcaat tgacttgtct cacgctttgg catttacttc acctaaaaaa
    15601 atctattctg ctacttacgc agacgcacat gatgctgact tagttgttat tacagctggc
    15661 gcaccacaaa aaccaggtga aactcgttta gacttagtaa acaaaaacct aaaaatcaac
    15721 cgcgatgttg tcacacaaat cgttgaatca ggatttaacg gtatcttttt agttgccgct
    15781 aacccagtag atattttgac ttactcaact tggaaattct caggtttccc taaagaacgc
    15841 gtaatcggtt caggtacctc tcttgactca gctcgtttcc gtcaagcact tgcggaatta
    15901 gttgacgttg atgcacgtaa cgtccatgct tacatcttag gtgaacacgg agattcagaa
    15961 ttcccagttt ggtcacatgc aaatgttgcc ggcttgcaaa tctatgaatg ggtaaaaaac
    16021 aacccagaaa tcgatgaaga agctatggtt aacctattct ttggcgtacg tgacgctgct
    16081 tacacaatta tcgagaaaaa aggcgctact ttctatggta tcgcagtagc attagcacgt
    16141 atcactcgtg ccatcttaga tgatgaaaat gctgttcttc ctttatcagt attcatgaat
    16201 ggcgaatatg gattgaacga catctacatt ggtgcacctg ctgttatcaa ccgtcaaggt
    16261 atccaaaaag taatcgaaat tcctttaagt gacagcgagc aagaccgtat ggctgcttct
    16321 gctaagcaat taaaagaaat tctagatgaa gcttttgcta aactagacgc tgaataatct
    16381 ttgaacatga cggaaaacat ctattcggtg ttttccgttt ttttatttta tcacccttat
    16441 ggtttctaaa ttttttcata aagtagagat aaccttcgtc ttttcctcta aattatgcta
    16501 tcatgagacc gttccaccct aaacttttac ttaaggagtt gtcgatatga caagatcttc
    16561 gcatcgaaaa aaatcgaaac atgttgcttt gctttcagtt cttggtgttc tcttagtgct
    16621 gatcagcttt tatacctatc gcagtacata ctacacaaag cattttttgc cgaatacaga
    16681 aataaatgga ataaatgtca gcaatctaac agtcgaaaaa gcaaatgaaa aattaaaaga
    16741 agcttattct gataaaaaac tggctattaa agaagatgga aaagtctggc aggaagtacc
    16801 aaaatccgag ctcggctaca aagatgattt cacttcggag ctttctccga tcctaaatga
    16861 acaaaacgga tggacttggg gaatgactta tgtctcagcg gcagaaaaac aagaaataga
    16921 tcctgtcagt caaaaccacc aaaaactaga cactaccgtt cagacattga caactaagct
    16981 gactgatttg aacaaagacc ggacacctac tactgatgca acgatcgaaa aagcaggtga
    17041 ctctttcgtt atcaagcctg aagtcaatgg aaatacgatc gacgtcgatg ctgcggtcaa
    17101 acaattaaaa agtgcggtaa actctggtaa agatacaatt gaattgactg aattcaaaga
    17161 aaaaccaaaa gtaacatctg aagacagctc actgaaagaa caactcgcat ccatgaatgc
    17221 tatcgcaaat gtaaaagcta cttacagtat aaacggtgag actttccaga tcccatcaag
    17281 tgatattatg agttggctga cctacaatga tggaaaagtc gatttagata cagaacaagt
    17341 acgtcaatat gtaacagatt taggcacaaa gtataacaca agtacgaatg acacaaaatt
    17401 taaaagtaca aaaagaggag aagttacggt cccagttggc acttacagtt ggacgatcca
    17461 aacagacagt gagacagaag cattgaaaaa agcgattctg gcaggccaag attttactcg
    17521 ttcacccatt gtgcaaggtg gcacaaccgc tgatcatcca ttgatcgaag atacgtatat
    17581 cgaagttgat ctggagaacc agcatatgtg gtattacaaa gacggaaaag tggcccttga
    17641 aacagatatc gtttccggaa agccaactac acctacacct gctggagtct tctatgtttg
    17701 gaataaagaa gaagatgcaa ctttaaaagg aacaaatgac gatggaacac cttacgagag
    17761 tccggtaaac tactggatgc cgattgactg gacaggtgtt ggtatccacg attcagactg
    17821 gcaacctgaa tacggtggag acttatggaa aacccgcggt tcacacggct gcatcaacac
    17881 cccaccaagt gtaatgaagg aattattcgg gatggtcgaa aaaggaacgc ctgtattggt
    17941 cttctaacaa aaataatata taaaaagaaa gcacccgtac gattggtaca ggtgctttct
    18001 ttttattatc taacaatgtg ctacttacat ttcagcagca gtaaagactt ctgaaacatc
    18061 atcatcgtct tctagcttat ctactagacg ttccaattgc tctttttgtt cctcatttaa
    18121 atcaatcgtc gtttgtggaa tcatcgtcaa ttctgcttgc gccaaagtga actctttttc
    18181 caactcatct cgtacagcag caaaatcttc tggtgcagta taaatctcga atacttcagg
    18241 agaagtttgc agatcttctg cccccgcctc taatacatcc tcaaacatcg tctcttcatc
    18301 aacagtcaac ccatcgcgtt caatagcgat atatcctttg cggtcaaaca agtaactgac
    18361 tgaacccgtt tctcccagtg atccaccgtt tcgagtaaaa gctactcgga catttgtcgc
    18421 tgtccgatta cggttatctg tcaatgcatg gactaagatt cctactccac ccggaccata
    18481 tccttcatac gtgatttcat cgtaattcgt ttcatctacg gcactcgtag cctttttaat
    18541 cgcccgttcc acattgtcgt taggcatatt tgcagctttt gctttgtcca caacaagccg
    18601 caaagctgga ttcatagatg gatcaggacc gcctgcttta gctgccatat aaatttcacg
    18661 agaaagtttc tgaaagactt tccctcgttt tgcatcttgg gcattttttc ttccttggat
    18721 attatgccat ttactatgtc ctgacatgat ttttcctccc ttgttctttt gagaatacac
    18781 ttctattcta gtactatttc acaaaaaaac aaagccacag ccaatctata aacgcttctt
    18841 gaatgccgca aaaagcacac ataatatcac agcagtcaga gcaccgatcg aatccaacat
    18901 gacatcttgg aacataggtg ttcgtccacc ggtcatcatc tgatggaatt cgtccaaacc
    18961 tgcgtaccct gttgctgaaa gccaagcaag gatcgctgtc agccaccaaa tttttagctt
    19021 ggggaaaaca cctaaaaaca agctgcctcc caaaataaaa taggtaccga aatgtgcgcc
    19081 cttccttaaa aagaactcta aaaatttggc gtagcccaga tggttgatac tcaccatact
    19141 tccaccataa ttaaaacgta tacccgctaa tgtgtcttta aatggctgat taggcagcca
    19201 gctttctaat cgcgaaactt gtgattgctg tccgtaagtt tgagaagaac tgtaaaataa
    19261 gattataacg actatcagtg cgattactaa ataaatattt ccgtttttca tttgtttttt
    19321 tgaagaattc ataagtcacc tctgtatttt atcttagact ttttttgaag aaacaacaat
    19381 cattttcata gagacttctt ccataaaaaa gtgctatact taattttgta ttacaatatg
    19441 aagggagttt tttcatgagt tattcattaa attggaatct agattctatt ttttccggag
    19501 gcagtcattc tgatgctttg aaccaacgga tgaagcaatt ggaagaccaa atgaacgagt
    19561 attatcaccg cgtaacaaaa tggtcaccta gttctgacaa tgcagagcag ttaaacgcta
    19621 ttcttcaatt gcaagaaacg attacgaatg gttttaccca atgcagcagc tatattactg
    19681 cattgctttc tgccaatgtc aatgattcag atgcaaaggt cttatcaggc aagctttacg
    19741 caatgctgcc tcgtctgcaa tcagccgaaa ctgtattgtc aaagaaattt gcagaaatat
    19801 ctgacaatga ttggaatcag atgctttctc aatcttcgtt tgaaacaatt gcttttcgtt
    19861 taaacgagat tcgtcgtgat ggctcacaat tattatcaga agctgaagaa aacattataa
    19921 atacgctttc tttagatggt ctaaatgctt ggagcagtca ttatgatacg attgtagcca
    19981 gcatttcgat tcccttcgaa caagatggac aagtagtcga attatctgct ggacaagcct
    20041 tcaataagat gatgggcgat ccagatccaa aagtcagaga aacacttttc gcagcatggg
    20101 aagaagcctg gaaagaaaag gcacccctct tcacggatac tttgaatcat ttagatggtt
    20161 ttagactttc tgactatgaa cttcatggtg tcacagattt tctacaaaaa ccattagagt
    20221 acaatcggtt gaagaaagaa acattgtctg tcatgtggga tacaatccag aaaaacaaac
    20281 aaccccttgt taattacttg actagaaaag ctaatctttt cggtaaagaa aaaatggaat
    20341 ggcaagatca agatgcacct attattttag gcgatttgaa agaaaaaacc tttagttttg
    20401 acgaagcagc agcatttatt atcgaaaact ttaacaaatt cagtccaaaa atggctaaat
    20461 ttgcccaatc tgcttttgaa aagagctgga tcgaagcaga agatcgtcct gggaaacgtc
    20521 ctggtggtta ttgtactgag ttaccagaaa caaaagaatc aaggatcttt atgacctaca
    20581 gtaattctgt taatgaggtt gcgacacttg ctcatgaact aggacatgct ttccatagca
    20641 gcacgatgtg ggatttacct tctttaaatc gcgaatatgc catgaatgtc gcagaaacag
    20701 caagtacatt cgcagaactg atcgtagccg atgctacatt aaaagtcgca aaaacaaaag
    20761 aagaaaaaat caatttgcta gatacaaaac tgcaaaatgc tttagctatg ttcatgaata
    20821 tccattcccg attcattttt gaaaatcgat tctatgaagc acggcaagaa ggattagtct
    20881 cagaagataa gatcacagag atgatggttg aggcgcaaaa agaaggctat caaggagctt
    20941 tagctactta ccatccatat ttctgggctg caaaattgca tttctttatt gatgacgtac
    21001 cgttttataa cttcccatac acatttggct acctctttag tttgggcatc tatgcttacg
    21061 ccaaccaaaa gggagcaaat tttgaagaag aatatatcca tttactacgc gatactgcct
    21121 caatgacgac ggaagatctt gcgaagaaac atttgaatgt cgatcttacc caaccagatt
    21181 tttggcaagc aggtatcgac atggtgactg aagatatcca aacgttcatg gaattgacag
    21241 aagaatttat ctgatttcta ttttatcaca ataaaaaaag agttagtcca gaatgggcta
    21301 actctttttg attaatcatt cttacatgct gacagcaaag tcaggggcaa accattgaac
    21361 tgaaccaact tttacgcttt cgcctggttg aggagcatgg atatattgtc ctccgcctaa
    21421 agcaatcgct acgtggtaag ttccgccttg tgacccccag aataacaagt ctccagcttt
    21481 tgcttgagaa actgaaattt ttgttccagc tgattcttga ggaactgtcc aaccaccgat
    21541 gtcacgacca gttacttgca tgtaaacata gcgtgtgaac cctgagcagt caaagccact
    21601 tggatcttta ccgccccaaa catatggagt accaatatat ttgtaagctt ctgctacgat
    21661 tgctgcacca ttcacacttc cagaaggagc tggagcaggt gttacaggtt ttggttgttc
    21721 aggtgttggt gttggcgttg gagccggagt actacttcct gtgccgtttc ctgtatcaac
    21781 actttgatct gttgatggtg ttgtaggcgc aggtgttgtt gattcttctg ttgttgaaga
    21841 ttcaggcact gttgttgatt cttctgttgc tgaagattca ggcactgttg ttgattcttc
    21901 tgttgctgaa gattcaggcg ctgttgttga ttcttctgtt gctgaagatt caggcgctgt
    21961 tgattcttct gttgaagatt caggtgttgt tgattcttct gttgctgaag attgtgtagc
    22021 tgatgatgat tcttcagtca ttgaagattg tgtagctgat gattcttctg ttgctgagct
    22081 ttgtgcagta gatgaagctt gagtagcttg tgcttcagcc tctgcttgtt cgcgtgcttc
    22141 tttttcagct ttttcttgtg cagcttgttg acgagcttgt tctgctagac gttgttgttc
    22201 acgaatacga gcttgctctg cttcagcttc tgctttttga cggttcaagt cagctttctt
    22261 gtcttcagca gttgcttgtt cagcagctaa tgaagttttc agtacgttca aatcagcttg
    22321 tttagataaa agatcgcctt tttgagattc caaagcagct tggttttcag ctaattcttt
    22381 tagtttagca tcgttttcag cttttttgtc ttctacagct tttttatctt gtttttgttg
    22441 ttccatcaag tcgttgtttg cttttaccat cgttgtcatt gcttgaacgc gtccaatagc
    22501 atctgctaat gaatcagcat tcaatacagc atcgatgtag tttgagcttg tgttgcttac
    22561 ttgtgcttcg cgtgcttgtt tttggattgt atcttcacgt ttttcaatac gttcttgtaa
    22621 atctgcaatg tcttttacta attgtgcaga ttcttgacgc aatgtatctt gttttgctaa
    22681 taggtcttgt gcttgtgtgt tgatttcaga aacttggctt tctaaggcat cgatttgtga
    22741 ttgtgcatct gcttgttgat ttttcaagtc tgcaatcttt tgatcttgct gttgtatctg
    22801 agaatcaaaa tcgtccgccg cggcagcgat cggtgatgct acggcagtca aggtcattga
    22861 acataccatt actgctgata ttaaactctt tttcactctt cattcctccg actggcttaa
    22921 tttaataaat atctaaatat atttaagtct ttcttaacga tataaaaatt gtaccatagc
    22981 agtgtgtcaa cgatataata gttatgttac aaaaatgttt caaaacaact tttaaaaatg
    23041 acaaaactgt tcgtttcaga aaatgatcat gaaaaagccg cgttaagtct agcatttctt
    23101 aagataacaa cgattctttt tcgctttcga cctaaaaata aaaaaagaaa ttattcatca
    23161 ctgaataatt tcttaaaggg aaaaatgaat aatacaaata taatcatgtt caacagcagt
    23221 gtaggtccta aaaaccttgt aataaaatac gtgttattta ctactgctaa tttaaaaatc
    23281 aactggacca ccatcgtaaa caattcataa cccgtgacaa agatgatcat actgaaaaat
    23341 tcagtaaaga tgttgacatg gataacgtct ttcattacat acatcaacca tacaattaaa
    23401 gggagagcta ctgcatagat accgataacg ccaatatagt aagcgtcata gatagccccg
    23461 agaacaatcg tggtaatcag taaataacgt ttttcaaacg ccatactgca acataataat
    23521 gccaatatca gaaagtgggc atttgacata taggtgcctt tcgaccactc ccctagcatt
    23581 cttgttaaat gaccatctat caacatcaaa aagaacaaaa cgattggcag atagtacttc
    23641 attgtttctt tcttcaacat ggactaatcc tccgctagcc gttggacaat cgtcacaact
    23701 gacacgtcat acatttctgc ataaggtttc acataaactt cccgatccag cccataacta
    23761 tctggtttcg ttttaataac cgtaccgatc ggcaagttag ctggagaatt ccctccaaga
    23821 ccggatgtct ggacaacatc cccttctttg atatccgtat caccagttaa ttgggtcacc
    23881 actaaggcat ggagcttttc atcatagttt ttaagcaaac caaacgcttc gccattagcc
    23941 gaagataccc gtactggaaa atgattggag ctttcattag aggatgacag taattcgatt
    24001 ttagacgaag ccgtattgac ctcgatcact cggccaatca atcctttttg cgacatgact
    24061 gccatatttg cttcgatacc gtctttagaa cccttgtcta cgatcagcat gtcctgccaa
    24121 ctgtcaggcg aacgagtaat gacatttgcc gttacttttt cgtaactagt cagcgtctga
    24181 ttcaactcaa gttctttttt caacgcttcg atttctctct cgtaattctt gttttgctgt
    24241 gaaacgtcat catagctatc gattttttct tttagccgct cattttcggc ataagttgaa
    24301 aaaagattat ggacagaatc tactccattt cgtacccacc gtactggggc agagattgtt
    24361 ttatcaacca ttgcaacact gtcattaacg atggattgga agaaattggg ctttccttca
    24421 ttcgctcgct gcgcagcggt cacacttagt atcgtcacca cgatgataac aataaccaag
    24481 gtaataataa tatttttatt aggattgaac ttcttcacaa agacacctcg aattaattag
    24541 actcttgtgt tcaattttat cactaaatgc cgtgaaacaa aaggtaagtt gctcctctta
    24601 agcttttttt tagcaaataa tagattctgt catttttagc ctgaaagaca aattttttta
    24661 tttgtttatg gatcaatctt tgctttgaat aactgcaatc ataccatctg aaaaagaaaa
    24721 ttctgcggta tctgaaagaa attcgttact tgcataagat gtcggatatt ctacttcttt
    24781 attttccaat acatacttgc tgccttttaa tgtaagattc gagacgggag tcaagcagca
    24841 ataagctaaa tacttcatat ctggtatctg tcttactata tgctctccag gcaaataata
    24901 agacaaataa ttttgacaat ctctgataat aagctgatgg gcaaagggtt tgaagcgagg
    24961 ttccatacct aaccagagat tggctaaaag atgatccaat cttccgcccg tagctccaat
    25021 caaagtgact tccgcttgcg ggtaacgttc aaatgttttt aagataccta actgtgtatc
    25081 tgtatcgtct ttttctgcag gggaacgggt atactctttg gcttttgcaa atacttcctt
    25141 ctgctcttcg ttcgacaatg aatcaaaatc gcccactgcc agatctactg ggtagcctgc
    25201 ttgaagcagg tacagcgcac cacggtctat cccgacatag gcatcgtatt tggacagcag
    25261 agactgcggc cagttttgcg gattacctcc agcagctaat aaaattttca tttagtctaa
    25321 tgcctctttt aatcgttcga tttgtccgac tggattttcg gctccataga tatatgatcc
    25381 tgcaacgaag acatccgccc ctgcatcttt acagattttt gctgtttctg gaacgattcc
    25441 gccatctact tcgattgcat aagtccaatt gttctctcta cgtagatcag cgagttccgt
    25501 aattttttct actgtttctt caataaagct ttgtccgccg aacccaggat tgacagtcat
    25561 gactagtact tgatcagcca aaggcaaaac atgtttgatc atggagactg gtgttcctgg
    25621 attgataact actccagctt taactcctaa gctttttgtc atttgaagtg cccgatgaat
    25681 atgaggagtt gcttcgacgt ggacagtaat gatatctgct ccagcttttg caaatgctgg
    25741 aatgtagttt tctggatcaa cgatcatcag atggacgtct aacggtaatt tagtaacagg
    25801 acggattgcc tgaacaatat ttggccctaa tgtgatattt gggacaaatt ggccatccat
    25861 tacatctacg tggatgtaat ctgcgcctcc tttttccact aactcaatgt ctctttgtaa
    25921 attggcaaaa tctgcactca aaatggatgg tgctatcttc attcttgttt cctcctattt
    25981 tcgtttacct tctttttttg catacattgg ttttcttttt tcgatttctt gtaagaattg
    26041 caaataattg tcgtaacgtg tctgtgcgat accacctgtt tctactctat gttttacttc
    26101 gcagccaggt tctttagcat gcatgcactc tctaaatttg caatggacag aagcttctac
    26161 aaattcagga aattgtctcg gcagttctcc tgcttcaatt gtcaaaaagt caatcgagct
    26221 aaatcctggt gtatctgcta ctaacccgcc atagatagga atcagttcca catgacgtgt
    26281 tgtatgccgt cctcttccaa gtgcatcaga aatctctcct gttgctaaat tcagttccgg
    26341 tgatatttga ttcagtagcg tcgattttcc tgctccagac tgacccataa agaccgatag
    26401 ccgttctggg aagtaacgga tcagttctgt cgtatcaccc gtatgattgg gtacaatcac
    26461 cggatagcct attttagtat aggtttgctt gatctcttcg attttttctg ggtcgtctga
    26521 caagtcggtc tttgtcaaaa aaataatcgg ttcgatcgac tcgtattcca aggtaactag
    26581 aaagcggtca agtagattat aagaaaatgc aggctctacg atacttgtga caatgattcc
    26641 aagatcaaca tttgtgactg gtgggcgaac taattgattt tttctcggca agatttccag
    26701 aagatagccg tctgtttgat tttcactctc aaagatcacc tgatctccga caagcggcgt
    26761 gattttccga ttccggaaat ttcctcttcc tcttgtttga taggtctctc cgtttgcgta
    26821 tacatagtaa aaaccgctga ttgcttttct gatttgtcct ttgataagtg ccacctccgt
    26881 ttttgtttat tttaccatac gaagaggaaa gtaggtgtta ttccatgcaa acttctcaaa
    26941 gtatatggct tgaaaattga tcaaaagaga tagaaacaca aaaaagagaa tgcaacaaat
    27001 caatgtcgca ttcccttctg atatatttta ttcagcactt attttgaact ttctgaggtg
    27061 ctagctgatg aacttgtgga gctggaagat tcttcttttg aagagttgct tgtgctactg
    27121 gagctacttg agctgctaga actgtctgaa gagtcttttt cagaactaga agacgctgga
    27181 tctggtcctt tagagtaaat tacgttcaca accattcctt tggtaattgt cgttcctgcc
    27241 cctggtactg tacgaatcac tttatcttta tccaccgtgt ttgaatattc ggaagtctca
    27301 tgcccgatat attctgcacc gacactagct aaataacttt gtgcttcgct ttttgtatag
    27361 ccggagatat cattcaattt aggctctgtt ccttggctta cgacaaaatt gatgtaagtt
    27421 tcttctgcta caacttcacc cgaggctgga tcctgactga tgatcgaacc tgatggtaca
    27481 gtgtcgctat agtcataagt agcactaata ttattgatat tgaatccttg tgtaacaagc
    27541 caattacgaa cagtttcgta gttttctcct acatgactac tcaaagtcac tgtttctggt
    27601 cctttgctga ctgtgaaagt aatcgtgtct gttttaggat cgacttctgt atcttctgat
    27661 ggagattgcg aaatgatcat tccgctttct acctctgaac tgaattcctc ttctttttga
    27721 atcaagtcag cagaaaaccc taattctttc aaacttgctt cggcatcttc ataactttta
    27781 ccactgtaat catctaattt gatttttttc gtgccgctac taatatacag agtgacttcg
    27841 cgtttttgct tgactgtcgt tcccgctgcc gggtctgtct tcactacatt accttcttcg
    27901 attttgtcat ctgcaatttc ttctgttttt gttgctactt ctaatttcgc ttttgcgagt
    27961 gtttctctag cagaagcttc agaaagatca gaaacatcag gaacaacaac ttctcctcgc
    28021 ccgccagaag caaagaatac agctgttccg attcctaaaa cagcaagaat agctaatagg
    28081 atcagccacc attttttctg tcctttcttt ttaggatcag gcagaacttc ttcctcctct
    28141 ggtaaggttt cgttttctgg cttgtctttt ggcagaggca tagatttgaa tgaatcaggc
    28201 attggtgcat cttccgtaag gggtgtgata acttttgttt cattatccat cacatgaggc
    28261 tgccattttt cttcgtttgc acgagctggt gataatactg tagaaagatc atcagacatt
    28321 tcctcggctg acttataacg gtctgcaggt tctttagccg ttgcccgtaa aatcacgttt
    28381 tccagtgctt gtggcacatt tggatcaagt gcttttatag atggaagatc atcttgaaaa
    28441 tgttttaaag caattgttac agcagattca ccatcaaacg gtacgctacc cgtcaacatc
    28501 tcataaagga tgatccctaa agcataaata tctgactgct tcgttgccat acttcctctg
    28561 gcctgctcag gggacagata atgaacagat cctagcatcg tattcgtctg cgtgatcgat
    28621 gtttcagata aagcgatagc aatcccgaaa tccgtgattt ttacaacacc ttcattatcg
    28681 atcagtacgt tttggggttt caaatctcgg tggatgattt gatggttatg agctaaagaa
    28741 atagcagaaa ggatctgctg cataatattt actgctgttt cataaggaac aggataatgt
    28801 gtttggatat atcgtttcaa gtccattcct ttgacgtatt ccatcaccag atactgcatg
    28861 ttgtcttctt caccaacatc gtaaacacta acaatgtttg gatgaacgag ttctgttgct
    28921 gctaaagctt ctctctgaaa tcgtcgaatc gcagtctggt cattttgaaa gtcaaatcgt
    28981 aagactttaa ctgctacatc acgatccaag attaaatcgt gcgccaaaaa tacatttgcc
    29041 ataccaccgc taccgatatt tccagtgatt tgataacggc catttaattt tttgcccaac
    29101 tcgatcatac actgtcctcc ttatattcga taagaagaac ggtgatatta tccgctccgc
    29161 ctgcttcatt ggcacgatca atcagctcag ttactttatg agacaatggg ccttcttgat
    29221 tgatgatcgt tcctatttct tcttcagaca gcatgttcgt caagccatct gaacataaca
    29281 gcagtcgatc ttttagttgc caaagatgag tagaaacatc tacttcgact gttccaggca
    29341 tgccgacaga acgtgttaaa atatttttcc ttggatggtt cactgccatt tcttcgctga
    29401 tttctcctga tttgactagt tcattgacta aagaatgatc ctccgtcaat tgcatgattt
    29461 tatcttcacg aatcaaataa gctctgctat ccccaacatt ggcaatcgtg aaccagtcat
    29521 ctgacagagc ggccgctacg attgttgttc ccataccgtt atattccggc tgttcttgtc
    29581 cacgctgata aatcagcata ttttcttctt gaatcgattg aatgaaccat tgggcgattt
    29641 tttcatcgtc aacagcttct tgtttttccc aagcttcgcc tagattcgtt accgccatct
    29701 gactggcaat gtctccggca cgatgaccac ccatcccgtc tgctaataca gcaagcttga
    29761 gacctgaacg gctggtaaat acactggcat agtcttgatt tgtgttacgt cgtcttccta
    29821 catctgattg atattcaatc tgcatttttt cacctcatta ctttttgcgc aaacaacaaa
    29881 tgaaaaatcc atctgtgtaa aactgatgcg gatacaaagt caacattttc tcttcgattg
    29941 atgattgtag cataggattg gtcaaaacat caatcctctc aaaatcaggg tgtctttcca
    30001 agaaagcaga aacaacgtct tggttttctt cttttaagat cgtacaagtg ctataagtca
    30061 gaataccgga agactttaaa gttggtgcgc agctttctaa aatttccaat tggatttttg
    30121 gtaaattcgc taattcatcc ggctgttttt tataacgaat atctggtttt cgccgaagca
    30181 gcccaagacc tgaacaaggc gcgtctacta aaatccgatc aaactgctct gcttcgaact
    30241 gtttactgac ttctctagca tccatctttt gagcgtagac agcagaagag acacctagac
    30301 gtgctgcatt atcttcaatg agtttgactt tgtgttcgtg tacatctaaa gcgaccactt
    30361 ctccaccgca tgacggatct aaaaaagaag caatatgcgt cgtcttacct ccgggtgctg
    30421 cacaggcatc taagacatga tgttcaggct cgatttgcat agacggcgct acaagcatcg
    30481 aactttcatc ttggatcgtc atgacgccat cgtggaacaa tgagctagaa gctaaatgcc
    30541 cttttttcgc tactataccg tatggactga cttggctttt ttcagctgtg atctcttctt
    30601 cttgcaaaac agtgatggcc tgatctctgg aaagtcggcg tgtatctact cgggcactag
    30661 catggctagg ctgatatagc gataaaccga gcttttcagt ttcttcgtat ccgatttgat
    30721 ccaccagtcg tttcgtcatc catagaggca ggctgatttt gacagaaagt ctttcgatag
    30781 gatcagcaat gccttgcaga tctggtgctc cttgacgctg ataattcctc aagacaccat
    30841 ttacaaattt ccctgtccca ggatttcctt ttacttttgc gatttcgacc gcctcgttta
    30901 aaatagcatg tgcagggact ttatctaaaa attctaattg gtatacagaa agataaagaa
    30961 gcatcttgac ccagtcatcc actttttttg ctttagcgat caatggtgca acgtaaaatt
    31021 ccaatagtcg ctgatggcta atcgtaccat agatcaattc tgtaaataaa cccacatctt
    31081 tttgatttag ctcgctttgt ttgatcatct cattcaacaa aagattcgag taggcgccac
    31141 ccttgttcac tcgctctaat gtcagtaatg ccatatatcg tactgatttt ttcatttttt
    31201 tagggatttt tttcattatt tcacctgctg accaatctga acttgctgac cgcttccgtt
    31261 aaggaaatca tgtacggcct gttttccttt accagctggc tgtaattcat tgatggctaa
    31321 gacggtctgt ttaccgcaag caatccatag attttttttg tctttccgga tgatcgtacc
    31381 aggtgctttg tctgtagttt ctgccaatac ttcaaccgat aataatttcc agcgtatgcc
    31441 ctcataggtc gtgaatgcaa ttggccaagg acgcattccg cgtacttggt tgtcaatttc
    31501 ttctgcagtt ttatgccaat cgattgcttc ttgttcccgt gtgatgtttg gcgaaaaagt
    31561 tgcttctgac tcgtcttgag gtactggttt ttgtccatcc aaaatattgg gcaatgtttc
    31621 tagcaatagc tttcttccta aaaggctaag cttttcaaac atcgtcccta catcatcttg
    31681 tttagtgatt ggtatgcttt cttgcgcata gattccgcct gcatccattt ttttgatcat
    31741 ttccatgatc gttacaccag tttctttctc cccattcatg atggcataat gaacaggcgc
    31801 acctccccga tatttaggaa gtaaagaagc atggacattg attgccccta attttggtac
    31861 ttgcaataac ttttcaggca agaattggcc aaatgccgca gtgacgatca catcaggcac
    31921 caattcaatg atttcttcca tttctggcga accggagatt ttctctggct gcagcacacg
    31981 gataccgtgc ttcagagccg ctttttttac cggtgtcggc gtaattactc gtttccgccc
    32041 aactggacgg tcgggctgtg ttaccactgc ctggatctcg taacctgatt ctatcagccc
    32101 ttcaagaatc ggcacagaaa aagcaggtgt gcccataaat actagcttag tcatcaagat
    32161 gttcctccat atattcttca agttcttctt ccgggatctg ctgtatcatt ttctcaatga
    32221 acaagatacc gtctaagtga tcgatttcat gctggaaggc acgggctaaa tagccaaaag
    32281 ccgtcacttc tatctcttct ccgtctcgat cataataacg cacggtcact tcatctgctc
    32341 gcttgaccgt tccatagaca tgcggaatac tgagacaacc ttctacgtca aggctttcac
    32401 cttttgcttc gatgatttct gggttaatca gttcaaattt ctcgccttca tctacttcga
    32461 tcacagcaat tctcttgttt tgtcctactt gaggagcagc gatccctatt ccgtcattcg
    32521 caatcattgt ctcatataaa ttgtctaata aagcgattgt ctcgtccgtg attacatcga
    32581 ttggctgagc agttcttttt aatttatcat tagggtggat caatatagga tagcgcataa
    32641 tttggccctt tcttaaataa agtttaatgg ttcgttgtca attgccacaa aaagaccttg
    32701 tcgctgttct ttctggcttc cattcaaaat ttcatccaaa acatgaggaa gattcggttc
    32761 atgtttatat ttgatgatta gttgataata atatcggttt ttcactctca aaatcatgct
    32821 gggcgttggc cctaatagca agctttgagg gcttaacact tcttttagct gattggcaat
    32881 ctgaaagatt ttctttgccg ctacttgttc ttcaggatgg ctggcggtta ttttcacagt
    32941 aaagtaataa ggaggatagc ctccacgatg ccgtacatgc atttcctgct gataaaactg
    33001 ctcataattt tgttttttag ccaattctat ggcgtaatgt tgaggattga atgtttggat
    33061 cacgacttcg cctgcttttt ccgctcgccc tgctcgtcct gaaacttggg taagcaattg
    33121 gaatgtatgc tcactagaac gaaaatccgg taaattcagg gaggtatcag cattcaacac
    33181 cccaactaat gtcacttctg gaaagtccaa acctttagca atcatttgtg tccctaataa
    33241 tatatctgct tcttttgcac cgaatttttg taaaatctgt tcgtgtgccc cttttcttcg
    33301 agtggtatct acatccatac gcaaaatcct agcttgcgga taaagttctc ggagttcctc
    33361 ttctactttt tgagtacctg ttccatagta gcggatttta ttgccgccac aattaggaca
    33421 gcgattggga ataggttctt catgtccgca ataatggcag cgcattgaac gtgtatccat
    33481 atgcagtgta agagagatat cgcaattagg acatggaaga acaaatccac agtccctaca
    33541 catcacaaaa gacgaatatc ctcgacgatt gagcagtagg acagtttgtt cttttttgtc
    33601 taatcgattt tgtatttttt cctgcaaatt agcagaaaaa gtagaggtcc gatgattctg
    33661 aaactcttca cgcatatcta cgatctcaat agctggaagc tgtgcagctg ctttggctcg
    33721 gtggtttaac tgcaataact gataaacgcc tttttgagct cgtgctcttg attctaaaga
    33781 gggcgtcgcg cttcctagaa ctattgggca atgatgatat ttactgcgcc aaattgctag
    33841 atctctggca tgataacgcg gtgtctcatc ttgtttgtaa cttgattcat gttcttcatc
    33901 gataatgatc agcccgatat ttttcagcgg ggcaaaaata gcagatcgtg cgcctactac
    33961 tacttgcgct tcttcccgct caatcttgcg ccattcatca tatttttcac cttgggaaag
    34021 tccgctatgt aagaccgcaa ctgattctcc gaagcgtcct ttgaatcgct cgaccatttg
    34081 cggagtcaat gcgatttcag gcactaacat aatagccgtt ttattttctg acaaaacatc
    34141 agcgattgcc tgaagatata cttctgtttt cccgctgcca gtgattccct ccagcaaaaa
    34201 gacctgatct ttttgctgct gacctgctgt gatgatttgt tcgacagcat ttttttgttc
    34261 tgcattcagt tccaatgccg tcgtttggtc aaatactcgg tctttataag ggtcgcgata
    34321 acgttctgat tctataaact ccagccagcc cttttctttt ccttgattca agacagcagt
    34381 actgattcct ttatttttga aataagcaat tggttcttct tggctcagtt cagataaaca
    34441 caaaagaagt tctttttgtt tcttggcatt ttgccgtaat tttgcccatt cttcttcgat
    34501 ttgcttttca gttaacaaag aacggaccaa acgaatcgtt tttactttat ttcgtgtatg
    34561 gacttcatat ttgatatcta ctaattgctc ttggcgccag cgaatcaatt gagaaaggct
    34621 gccttcttct tctgcttcct tccaggaaat cacttctcgc tgatcaaaca atacctgaac
    34681 ttctggtgtg tctgccaaag gatagatcaa tttatcatat tctgctttca tcacactcgg
    34741 aagcatcgtt tgtagacaag tgattttgaa tgcgtaagta atttttttca tataatcagc
    34801 taatgaaagt aattcttcat tgaccaccgg agcaaggtct agcaaacgaa taactgcctt
    34861 caaagatgga ttagctgatt cttctgattt ttggatagcc ataacaaatc cttggacatg
    34921 acgattgcca ttaccaaaag ggacttctac tcgcatcccg acttgcaaag ctgtttccat
    34981 ttcagaggga accaaataag tgaatggctg atcggtctgc atagtaggta cgtccacaat
    35041 gacttcagct gttaatgtca tggttcgttt cctccttttt ttgtcttttc ttatcaagcg
    35101 acaaaaagtt ccttgcctat tttactagag atcgatggat ttgtcttatc ttctgccaaa
    35161 tcctaacaaa aaattaacag cagataagaa aaaaaagaac tgtgtcaaaa agttcagaca
    35221 cagccttttc taccagacaa taagtggcga atgtttgaaa aggaaacagc tgggtttgct
    35281 tttcttttta gatgttcgct ctatttttct ttattacatt ttttgctctt cacggatacg
    35341 tgattccaat tcgtgttgtt cacgctcttt ttgtgcttga cgttcctctt cttccatctt
    35401 caaacgttca cgttttaatt caggatgtgg atcattgact acatttcctg catcgatttc
    35461 ttctaaagct tgaccgacgc tttttactga atcaaaagaa tctagagtag gctgtgcact
    35521 tgcatccaat tcatgcgcgc gtttacttgc taaaatcact agagaatatt ttgaatttac
    35581 acgatctaat agtgagtcaa ttgaaggttt taacatcata agactacatc tcctttaaca
    35641 ttttaatata tttaccgatt acgcgatcta ctcgaaaatg ttcactggca ataatgtttt
    35701 tgattcgttc aactgctagc ggcacttcat catttacaac agcatagtcg taaagagcca
    35761 tcatttcgat ttcttctttt gcaactttca ttcgtttttc gattacttca tctgcatctg
    35821 ttcctcggcc tacgatccgt gattttaatt ccgctaaatc tggcggagtc aaaaagatga
    35881 atacaccatc gggcactttt tctttgactt gttttgctcc ttggacttca atctccaaga
    35941 aaacatcttt tccttcatcc aatgtcttgt tgacatatga caaaggagtt ccataataat
    36001 tcccgacata ttctgcgtat tccagcatct ctccagcttc gatcattgat tcaaactctt
    36061 ctttgcttcg gaagaaataa tcaacgcctt cgacctcccc ttcacgcatt ttacgggtag
    36121 tcattgagat cgaatactga aaatcattat cctcactctc aaaaattgct tttcgtactg
    36181 tgccttttcc tacaccggat ggaccagaca acacgattaa taatccacgc tctgacatga
    36241 cgacgtcctt tcaactagtc tttttgtact tatcgtttat tttgcacttt ttttctgcaa
    36301 ctttcaaggt tatctttaat tatagctaga aattggtaaa aaagctaccc tgtaccaaga
    36361 ggtcgtaaaa aagctgtcct gcatcaagta ataagaacag ccaaacataa acagcttctc
    36421 atgtttttcg tttggttggg gttattatcg cagatgcaag cttttgaacg ccgtgtataa
    36481 gaggtcgtga agaagccgat tagtttcgag tatgaagaaa aattttgtga aaatagtttc
    36541 tcacattttt gcataaattt ggcttaatac cgagaaaatg gtttttgaac atcacttatt
    36601 caaaataaaa aaagattgtg acacgaattt tgtcacaacc tttaaatgta ttcctagagc
    36661 aaagaagcta agcgaagaaa tccaattctg tttatttatt gtatttcttt gttcagcatc
    36721 cttgctaaaa gcccatattg gacaaagtat tcactacacc catgatctca tcttttcgag
    36781 aacgtgcttt ttctgtcgga tcttctacct cttctgtgtc aattcgaaca ataaatttcc
    36841 gattcatcgt cacgtaagca ccgtcgttga tcaaaataaa cagttcgttc tcattatagt
    36901 ctgcacaggc atctttccat acgtcaaagc cgtttttttc agaagggata tcccgtttaa
    36961 ccacagtttt tccacttaat tcgtggaacg ttacgtcata atatttcatt atctctacct
    37021 ctttcgtcat tcaatatttt gtacttgttc tctgattttt tctaagatcg tcttagactg
    37081 gacgacttga tttttaattt cgattgcatt tgatttggaa ccgatcgtgt tgatttcccg
    37141 attcatttct tggatcagga aatctaattc ccgacctaca ggacattgtg cttcgataag
    37201 ttcatctagt ttttgtacat ggatcgccag acgatccagt tcctcgtgaa tatctccgcg
    37261 ttccaacaag atggctagct ccgtcaacaa ccgttgctca tcgactttcg ctccaagaaa
    37321 ttcctccagc tttttttgat accttatttg aaattccttt tcatagattt ctacaaaact
    37381 atcgagtttt tttagtactt ctttgaattc ttcagtattc tttttcaaca ctaaagctaa
    37441 agcttgacct tctttttctc gactcgcctc aatagaatca agtgcgcttt tcaatgtatc
    37501 tagagctaac tgtgccaatt cttccatgct ctcttccggt ttttcttcta ccgttacgaa
    37561 gttttcgtcc agcacgaagc gttccgccat ccgactaatt gacacttgat tttgactacc
    37621 gtatcgttgt tgaattccgt ctgtcaattg agtgataagt gaatcaatca attcccagtt
    37681 gatgacgatc tgtttttcgt ttttgccaat ataagctaga ttcacaaata cttcgattcg
    37741 accgcggctt aactgctgtt taataagctg acgaaaatcg ttttcaacaa agttcaggat
    37801 tttaggactt cgtatttgaa catctagaaa acgctggttg acacttttta tttcaatatc
    37861 taactgatac gcttttgttt cgcggatggc tttgccaaaa ccagtcatac ttttcattcg
    37921 acagggtcct tcctcattac atattttcta ttgctttttg cagttgttca aactcttcgt
    37981 tcgtcaatgt caggcctttc cccatttttt catggtctgg cgcccaatct cgaagatcga
    38041 attttggcgg gcgaccattc cagctgacta ggttcagttc tttacgccac cccttgttgt
    38101 tttctgacaa tatagcgatt tcttctacga tttcatatga aaattcttgt gccatggttg
    38161 ttcctccttg agataatata aatataggat cagtaatgtg tcaactggtt gcttaagcaa
    38221 ttcatgctgc aaacgatcct tgcgttttcc ttgtagcaga taatcaaaat aaacagtttt
    38281 cgtgcgctca ttttgtattt tttgctggta ttttcgatac cttcgccatt cttcagtact
    38341 taattgacca atttgtgcca ataaatcatc cggcatactt tttatatctt gttggcagat
    38401 catttgaatc atcatgtcta cttgatagat ttcattgggc agtaaaaaat gtttttttgc
    38461 taaaagttct agcatcgcca atccgcatgt ctctggtacg accctgttct tatataaatt
    38521 acgcagtttt tgccaaaatt ttatgctttt gaaaaaagat aattctaact gaatttcttt
    38581 tgttaagtaa gacaaaaatc ctcgccagta tgttcttgca ggtttaacag aatgaaatgt
    38641 ataccagttc ccatagtaat agcctaatcg ttttttcatt tccagatagc ctaattgtaa
    38701 atttaattgg ttcctttggc tatcaaaaag cagaaagctg cctagtgtcc acaatgtttg
    38761 acatttccaa tggataaaaa tgtctggcat acgaattttt ttcgctgggc ctggcccttg
    38821 cacatctacg acaaatgcct ctgttgctcc tttgttaatc gcaatatcaa taggtatttt
    38881 gttgcgatac ccaccgtctg catatttatt ttttccgatc tttcgataag ccatagcggg
    38941 ataaaatgaa gcagaggcga gtatccaatc tggtatttct tctgtttttg tctgttggat
    39001 atcagtcact acttccgcta gttctggcag tttagtagac accgtatata aacgtatttt
    39061 gggattatgt ttgagtaagg acaaatccaa attgtctttg accatctttt ccaatggagc
    39121 agaagaaacc ccgccttcaa ttagtgctgt tttcgtcatt tggcgtcttt ctcgatagaa
    39181 gcgttcacga aggtcttcaa ctgcagccat ttctggtaac gcaagcactt gtcttgtcga
    39241 cagctttttc cataaattca cagctttttt catatctcct tgtaaaatca atgcgccgtt
    39301 caacgcacct accgaggtac ctgtgataat ctcaaagttg atatcatgtt ccttcagcgc
    39361 ctgccacaca ccaatctgat aagcaccgtg cgctcctcct ccacctaaaa caagtgctgt
    39421 atgatagacc agctttttat ttaattgtgt ggttccttga tagccgtttt tatgaaaaat
    39481 acgcaaccat tcttctgata gaggagattg aaataaaaaa caacagtttt ctttaataaa
    39541 aaaacgagcg caagcttcca tataccgaaa aaattttttc cattcaaaag tttcaccttc
    39601 aatcaacaga ttattaaaaa taacatctcc tctactttta gcaatagtaa aatagacggt
    39661 atattttttc gatactgctt taaaagcaca ttgctttttt gcagaacgcc aaaaagcgtc
    39721 tctactttct tgatgacttt gaaaaaatgg aaggggttgt tttgcttgct ggataggcga
    39781 atacagcgac attttctgtg aaaagtaaac tttttttatc tccatcgcgc tctccttttt
    39841 cttcaagtat accagtacgt cataaaaagg tcatgagtct cgtattcgac tttgtagatt
    39901 ccataagaaa acaattcata aaacctcctt taaataaaaa aatacaaaaa tcacaaaaat
    39961 taattgaaaa tgagagtgtt ttcaatgaaa acgtgatttt gtaatggaat tttaaaataa
    40021 aagaaacaag cttttttaat gcaaatgttg taaaataaaa gagattaaga ggaaataaga
    40081 ggaggaacat gatgactgag tcagtttatg tacatattga taccacaagt aatgctgttt
    40141 taacaaaagg gctgactgct actgactttg caaacagtat cgtgcatttt cctcaaaact
    40201 tgctcttgct tgatccatct gcttctgctg gagaatatga atcccataca gggctgaaag
    40261 taatccgtgg tacagaaaat attcagcgtt tcttttcctc ttctcgtaac aggcgtggat
    40321 tcgatgaatt gaagtggatc gactttactg atttaacgat gctgaaagaa ttgacacctt
    40381 tagaaatttc agaattatta tattttggtc atatgaaaac acatcttcat tcgccgtttt
    40441 tttataaact acagaataat tttgtatact tcgatcttaa tgatcagctg aatcggattt
    40501 attatcgcta tttagatgaa ttttatagga tttttgctag taaattaagt tcgatcttgt
    40561 cagataagat caatgtaaaa aaatcttttt tcaaaaaaga aacacctgtt gaaaaaatca
    40621 gtgtggaatt attgaaagaa atgcgtaatt tgatgcagga agggatcatt ttttcttttg
    40681 atcaagttgg tttgatcgat ggtaaatatc gtatcccaat ctatgtgatt gaagattata
    40741 tttggaaatt aaaaggcacc cggtatcgag aagaggaaat catcgctact ttaatctact
    40801 ctgtcaccga tcgacactgg caattggtag aagatgccga tctttcttat ctgccattga
    40861 agtagatcta tttcttatca aaaaataaaa aggaaacaag aaactaggac atgattctgt
    40921 ctcaagtttc ttgcttcttt tttatttaca gctcctgtcg tttatctggc acaaaaatcg
    40981 aaggagtcgt ttgttggatc cctaataaaa cgatgatcgt tacaatagct gtactcaacc
    41041 cgctggcacc attcattacg aaagaaaaga gccaaggatt catcccccat agtgcatacg
    41101 ctccccagaa aaccactcct gcaataaaat gccaaaaata tctggaaagt gcgccaatca
    41161 atgcaccaaa cacgatgctt tttctaattt gtttcgtatt atcgtctaca tagccttttt
    41221 ggacatttga agccatgagc cctgctgcgc ctgcaaaagt ataggcaatc gggtactcaa
    41281 tcaacacttg aacgacactt aaataataaa cttgtcccaa cggaaaatgt aggatgcccc
    41341 aaatgaaacc tgccagcatc cctgcctttg tccctcttct taacgcaaaa agagtcaatg
    41401 gaatctgccc taatgaaata gaaaaactgc tgccgatctg gatcggaatc aacgacaaca
    41461 ccatcgctaa cgctgcaaca atcgttcctt ctacccaaac tctatttttt cccataaaaa
    41521 aacttcctcc tttgaataaa aaagtcacaa aggaggaagc agctttcgaa aatagtcaaa
    41581 cagctgttta ttcacaattc ctacgctcgc ataaccgata caggtacgaa gggtttagaa
    41641 atagattcaa tctcagccca attggactcc cctttgtgag tttatttatt atttattttt
    41701 tgtttcaaac aatggactaa ctggtttgtt ttcatggata cgtttgattg catctcccat
    41761 tagtccgcca acacttactt catcgatttt gtcgatttta cgatcttctg gaagatagat
    41821 cgagtctgta acaactagac gctcgatagc tgaatcttct attctttgta aagcagggcc
    41881 ggataatact ggatgtgtac atgaagcata cacactagtt gcaccagctt ctttcaaagc
    41941 attggcagcc aatgtgatcg tacctgctgt atcgatcata tcatcgatca gtacacaagt
    42001 tttgcctttg acttgtccaa tgatattcat tacttctgca acatttgctt ttggacggcg
    42061 tttgtctatg atcgcaatcg gagatttcaa aaattccgct aatttacgtg cccgtgtgac
    42121 accaccatgg tctggtgaaa caacgacaac atcatcgcct ttgattcctt tttctaagaa
    42181 ataattggca ataagtggcg cacccatcaa atgatcgact ggaatatcaa agaaaccttg
    42241 gatttggact gcatgaaggt ccaaagtcag cattcttgtt gcaccagctt tttgaatcat
    42301 gtttgctact aatttcgctg tgattggttc ccgtgcacgt gctttacggt cttgacgcgc
    42361 atagccataa taaggcatga ctacgttgat tgtttttgca cttgctcgtt tcagtgcgtc
    42421 aatcatgatc agcaactcca tcaagttatc attgactggg ctgctcgttg attgaataac
    42481 atagacatgc gatccacgaa tactttcttc aatgtttact tgaatttctc catcactaaa
    42541 ttgagtaaca gatgattttc ctaactcgac gccgactgct gctgcaattt tctctgctaa
    42601 tgggcgattg gaatttaacg caaaaatttt taatcttggg tcaaagtaat gttttgacat
    42661 ggggacctcc actttctttt tcacaactct agaattctac ctaataaata gtagtcatat
    42721 tttttaaata aatcaagtga ttttgcaaaa gtcttagttt agataaggga gtttttttgc
    42781 atatccttct ttattgactt gtcttgctct cgcaattgcc agcgcatatt ctggaatatt
    42841 gtctgtaatt gttgacccgg cagcaatcga actgttttta gctacctcga caggtccgat
    42901 gatattcgtg ctggaaccaa taaagctgtg gtcgccgaca gtcgtatgat gtttgttctt
    42961 tccatcatag ttgacgaaga caacaccaca tccgacattg atttcttctc ccagcgtagc
    43021 atcaccaaca taagtcaagt gaccgacttt agttcctttg ccgatttgag catttttcac
    43081 ttccacaaag tttccgatat ggacgttttc gccaatttca gcttttggtc gtaaatgtgc
    43141 ataaggtccc acatctgcat gtttcttgac atggctgctt tcaatgactg agttttcaat
    43201 gacgacgtga tcctcaatcc tactgtctac gatttttgaa tgtgacccga tgacacaatc
    43261 agaaccaatg actgtatttc cttggatttg tacacctgct tcgatcaatg tgtctggacc
    43321 gatttctacg cctgcatcga tgtatgtcgt tgctgaatcg acaaagctta cgccattgac
    43381 catatgcatc tggttaattc gctttctcat gatctcattt gctttagcta acgcgatccg
    43441 atcattgact cccattgatt cttcaaaatc atccgtttga taagcagcaa ctgtatggcc
    43501 ttcttctttt aagatttcga tgatatctgt tagatagtat tcaccttgag cattgtttgt
    43561 tccaactttg cttaacgcat cgaacaaagc ttcgttgtca aaacagtaag ttcctgtatt
    43621 gatttcttgt acaagagctt cttctggtgt tgcatctttt tgttccacga ttttttctac
    43681 gatcccaata tggtcacgga tgattcgtcc atatcctgtt gggttttctg cttgtgccgt
    43741 caagatcgtt gcacttgcat tttttccttg gtgatattca aaaagattgt tcagcgtttc
    43801 agtagtcaat aatggtgtat cgccactgat aactaacgtt gttccttttt ttccttccaa
    43861 gaaagaagca gcttgcaaaa cagcatgtcc tgttcccaat tgttctgctt gtaacgcata
    43921 tttactgcgt tctcctagtt gtgctttaac ttgttctgct ccgtgtccaa cgatcgtaat
    43981 cacttcatct ggtttcgttt cactgacccg attaatgata tgttctacca taggtttacc
    44041 agaaacggga tgtaaaactt tatataattt tgacttcatg cgggtacctt tccctgccgc
    44101 caaaataatt gcataacgtg cgtccaactg cgtcactcct aaatatagtt tgctgatttc
    44161 agtttatccc agtataccca ttttttcaac aaaacaagtg attttctttt atcccagcgt
    44221 tttctggata aaaaaataga tagaccataa cgggcctatc tatttggtaa ccggtgctct
    44281 cctgccaact cctcggtcac aagtcacact actcataaaa catgaaaagc tgttttttga
    44341 aatcgtttct tgtgacttag agttaaacgg taggcttaca acctcttcat taaacgtttt
    44401 tcaatcagct gaccttcggt attagctcaa taaacggcaa aatatgagga gcaatttttc
    44461 cgtttattga gctaatactc aggacaggac gctgatttca caacctctag ataaacggtg
    44521 ttcaaaagcc agtttctcgg tattaagcca aatttatgaa aaaatgtgga aagccatttt
    44581 cacaaaattt ttcttactac tcgaaactaa gcggcttttt cacaacctct tattctgaaa
    44641 aataatttcc tggcacaacc gtaatatttt tcgtttgagt atctacatct ttgacataaa
    44701 gcagtgacgt ataatcatcg atggcgcgat gaccgttgaa ttttgattcg gcaaatacag
    44761 tgatgccaac aagttctgat tcgaattctt caatcaagct cttcattccg ttgactgttc
    44821 cgccgccttt catgaaatca tccactacaa ggacacgtga accacgtttc aaacttctct
    44881 tagacagttc catcttttca attctttcag aagatccaga cacatagttg acacttactg
    44941 ttgatccttc tgttattttt gagtcccggc gtacaataac gaatggcgca ttcagataat
    45001 aggacacagc ttgggcaata ggtacgcctt ttgtggctac agtcattaca gcatcgattt
    45061 tttcaccaag gtatttagaa gcaatgattc ttccaacttg acgcagcagt tcaggttctc
    45121 ctaacaaatc agagagataa acatatcctc ctggaagcag acggtcttgt tctgatagac
    45181 gttctgctag agattcaata tattttttcg cctcttcata tgaaatttca gggatgaaca
    45241 atacaccacc tgcagcaccc gggattgttt ccaaggttcc ataccctcgt tctttaaatg
    45301 ttttcttgat gattcccaaa tcttcactga tagaagattt agcagattca taacgttcgg
    45361 caaagcttgt taaggaaatc aattcatgcg ggtgattcaa taaatactgt gtcatgtcaa
    45421 ctagacgttc actgcgacgt actttcacct attccaactc cttttaagaa attttatcgt
    45481 tttacattat ataagttttt cttcaaaaat tcgagcattt tatgaaaaaa taagaaaata
    45541 gttcggtatt tattaaaata aaaagaagcc tataaaaaat ttttcgtttt tataaggctt
    45601 cttttcttta taatttagtt attttttctt ctgaaggcaa ccaatattcg tttgatgata
    45661 tttacaatca ggaacaaagc aataaagact aatgtgattg ttgctcctgg aggtgtatct
    45721 agataaaacg agctagttag tcctgacagc atccctagaa atcctacaca aacactgata
    45781 gcaatcacga cattgaagct tttgcctaat cgcataccga ttgctgcggg aagaaccatg
    45841 attgcggata tcaaaagtgc accggcaatc gggatcatca cagcaatagc cacaccagta
    45901 atcacattga acatcatcga catcaaatga acgggtagcc catccacgtg ggcggtatct
    45961 tcatcaaaag tcagaacata catcggccgt ttgaataaaa agaacaatat accgatggca
    46021 acgaataaga ttcctaaaaa gatgacttgc tcccatgtaa tggtcacgat cgaaccaaac
    46081 agataggatt ggatacttgt tgctgaattc cctccgctca aattcatcaa tactaacgct
    46141 agcgccaaac caccagccat taaaatggca atggagattt ctgaataggt tcggtagatc
    46201 gttcgcagat actccaatat cacagctgct aatacaacga ctagcatggt cgtaagtgta
    46261 ggattaaggt tcaaaaagaa tcctaacgct acccctgcta aagagacatg agaaagcgta
    46321 tcagccatca gcgattgtct tctgatcact aaaaacacac ctagcatcgg ggcgattccg
    46381 gcaatgaaga tagcggcaag aaacgctctt ctcatgaatt catatgaaag caacgccatt
    46441 gagaatcctc ctttctcacc agtcggatct gtctgtccgc atactgtttg atatcttcat
    46501 gatcatgggt gatcattaga actgatttcc catggacatg cgcactatgt cggagaagac
    46561 gataaaattc atttctggat tcttcatcca tacctgttgt tggttcatca agaacaaata
    46621 agtcagggtc agtagcaaaa atacgcgcta aactgattcg ctgcttttgt ccgccagaca
    46681 attcacctat tcgccgattt cgcatatccc acatccctac agcttctaaa gcacgctgta
    46741 catgttgatg atctttttca gagagacgct tgaaccagcg gcctcttgga tatcttcctg
    46801 aacggactaa ttcgatcacc gtactaggaa aacctgcatt gaaagatgca acttgttgag
    46861 gaatataacc gatacttaat ttttttcctt cttgattttt ctcagcaatc tttatcattc
    46921 cttttgaagg tttcagtaat cctaacgatg ctttcaccaa ggttgacttg gctgctccat
    46981 tttctccagt taaaatgaca aattcacccg catctacatg ataagagaca tcttctagta
    47041 caggttcatc atcatagtaa aaggtcaaat cagatatttc gatataacgc accttacaac
    47101 ctccattcta aaacttaatt gatactttct tttaaagctt tcagattttt ttccatgaca
    47161 gaaatataat tttcgcctgc tttgatttgt tcattagtca agctttctaa aggattcaac
    47221 acttctagct tcactcctgt ttcattcgac aatgtctcag ctaccttcga agaggcattt
    47281 tcttcaaaat aaatcacttt gatgttgttt ttcttcacaa attcttttag ctcagctaag
    47341 cgacttggtg tgggttcctg atctggtgaa acgcccgaaa tcgctacttg ttttaagtcg
    47401 tattgattcg ctaaataagc aaaagcagca tgctgagtga caaatgtgcg gtttgttgca
    47461 tcttttagct catcggagta tttttgatcc agtgcttcaa gctttttgat ataagcatcg
    47521 gcattcttcg taaaagtttc ctttttatct ggatattttt cgcctaattc ttcagcaatc
    47581 gttctgactt cttttatagc taataccgga tcgagccaaa catgtggatc gtattcatgt
    47641 gaatgaccat gcccttcttc tgattcttca tgttcgtctg cttcttgcga ttctaaacgg
    47701 tcgattcctt tagctgcttc gatcatcaag gtctgtttgc tatccactga atcctttaac
    47761 gaatccacaa aaaattccat gtctgaacta ttatacacga aaacatctgc atcggaaatt
    47821 tctgctcttt cttttgcact tggttcgaaa tcatgcggct ccgttccagc agggatcaaa
    47881 agtttcacgt ttccttcatc ccctacgatc tcttttgtaa aatcatacat cggataaaaa
    47941 gtcgtaacga tcgtcaaatc ctcttttttg tctgcttcct tgttcgtatt tccacatgct
    48001 gcaaacagta aagcacctat cattaccgta gctgtcaaaa gatatttttt catttatatg
    48061 ctcctttcaa tgaacaaatc ggaattttac gatttattaa cgtgaactaa tgtatcaaaa
    48121 taaaaaaaca cagtcaatag aaatccatca atttctgatt taagcatgta tattcttgat
    48181 taaatcgtaa aatggatgag ttgctgctca tatctagcca tttcactaca ataaaaaact
    48241 gaagaatacc attttaggat tcctcagttt ttcatcacct attttacttc ttatatttta
    48301 ttgcagggtt cgtactaaat aaacttcctc acaaaaacct tttaacccat tatatactct
    48361 ctgcgctctt gaataatgtt gacacaaggc aaaaacggtt ggtccgcttc cactcatcaa
    48421 tgcagcatct gctccgtact tgatcatacg ctctttgatt tgatggatga ctggatgccg
    48481 ctttcccgtt atatcttcta aactgtttcc catttttttg atcatctgct gataatcttg
    48541 ctctcggatg gcttcagcaa gtccgtggat gtcttgatgt tggatggctt gcgtatctac
    48601 ttctctaaac actgtacgtg tagagacact cattcttggt ttgaccaaca caacccagca
    48661 ctgtggcata gaaggtaata cttggatttc ttcccctttg ccaccgacaa atgccgtact
    48721 tccgtagaca cagtaaggaa catctgtacc gacttccaaa ccgatttctg ccagttcttc
    48781 taacgaacag cccagattcc ataattggtt gatccctctt agagcagctg cacagtctgt
    48841 acttcctccg cctaatccag ctgctactgg aatattcttc ttaacaaaga tcttgacacc
    48901 ggttttgata ttgtatttat tttttagcaa taaagcagct tgataaacat tgttccgttc
    48961 atctaccggt aaaaatgctt tatttgtttc tacaatgatt ttgtcttctg ttatttcttc
    49021 aaaaaataga tgatctgcca agtctacgct cgacatcacc atttccaact catgataccc
    49081 atcttgccgt ctatataata catccagtcc taaatttatc ttggctggcg ctttttcaat
    49141 caattccatc ctctcaccca ttcgaaaagt ctaagtcgca agtattctat catagggaaa
    49201 aataaaagta tagcttatgt ctgcctagtg gttgattttg tcatcgtttc aacagctttc
    49261 acagaatttt ctcttcataa tccaaacaag ttctagtgat tcttataatt ctgtgagcac
    49321 ttcttctgcc aaactcggaa catttccttt ttcttttcct atcagactga gtagctgcat
    49381 ggtagcaaat ccgaaattac ttcctttcct ataagcaaaa taacgtcctt cccaatgatc
    49441 gacatatttt aatttctttt cataagcaga ctggaaggaa tagaaaaaat taccataatt
    49501 atatacgata tgcatgactt tttcttgaaa gaaagaaaaa tctgtttctc caacattagc
    49561 taatggagct aagcctagat tggcttccga gtagcccatt ttctcgtata caataaacag
    49621 attggctaac agatagtcag ctaattcctc agattcttta ggtaagaccc gcaacaaatc
    49681 atatcccgcc tgcttcttcg tcatcggctg agcggtgatg aagccaacga ctttttgatc
    49741 tttacggaca ataccgacac cgctagataa tacatatgat ttatcaaatc gtcccacaga
    49801 gaagtatttt tctcgtgcac cttgaagcca tttttgtgaa acttcattta cagcttgatg
    49861 taattcttcc ggcagttgat cgtaccaagt aaaatggtat ccttctttca tcaaacgttg
    49921 atattcaagt tgctgactca gccgagacaa gtcttctgtt gctagatcaa caatccctgc
    49981 ttcacctact ttcgtgaagt gaaaacccaa ttcatgaagc atcactacgt agctttctga
    50041 tatcttataa aacgcaagct gatagcctaa acaatctgct tgtttcataa actgattcgt
    50101 tgctgcccgc atcattttag gattgccaac tggatctcct aaaacaaagc atttgtttcc
    50161 tttgatctga taaccaaaaa cgacttgatc ttggtcgtct tcttggtaat aaaaataact
    50221 gtaacctgac aagcgaagct gatggcttgt agctgtgcct ccgtatgttt ccaataaacg
    50281 gtcaaaacgg tctttttcgt actcactgcc aagcttcgta gtatcttcag ctaaatattg
    50341 ataaagcgcc actaaagcta gcaatgagat gcccaaacca ataagccctg agaaccacac
    50401 atcatctgaa ggaaacaaaa taaaatgatt tcctgttatt cgaccgacta aaaagcgtcc
    50461 gctgtgatag cctgcaattg cgtaaacgat aaacaaaaag ccgaacaagc tagcatcaaa
    50521 gattatagct ccccaagaat aaacgaattt tgttcgataa aattcttttc tggataacca
    50581 cacagctaac agaatcaaga catataccaa tatcaactgc caagataacg tacgtgtgat
    50641 tgtatttaaa ataccaaaag atagtaaagc aatcgtaggc agaaatgcct ttttgacttt
    50701 tactgaaagt gctctagcaa ggccgagtaa taaaaagcca accaatagat tcactgtctg
    50761 atctaaaaaa ttgaatgaga acggcaataa tacttgaaat agccgactga ttgcagacag
    50821 attagtgatt gttgatagaa gtaccatcat gatccccgca aaatagacag cacttacgag
    50881 cacgatatgc gcaacctttt ggaaaaacag tcgcggaatg ttatctaaaa atcgattgat
    50941 tttgactcct gtatgtgtga tgaacaatag aagtcccgtc ataaaaggta gaacataata
    51001 aaacaaccga taatataata gccaaactac tgctacatct tgccgaacag acaattgact
    51061 cattcctaag atcatcaaaa cgtcaaaagt ccccattcca cctgggacca tcgtcagcat
    51121 tcctatcaat gtagcaatca tgaacatagg atagatcgaa agcatcgaaa tatctattct
    51181 catcaacttg ccgatgatca aaaataccgc aagggctccc gtccattgtc caaaggatgc
    51241 cagtagcaga ccaagcaagc cttttggata aaactcgaag aacaatgtct ttctctttag
    51301 ataagcaaaa aagaaaatga ctggtgctaa aaaactaccg ccgaaaagcc agatccaata
    51361 gcttcgaaaa atactttctg actgaataaa aaagacatca ataaatgtaa gaaacgacca
    51421 tacagacaaa ccagtcagca taaatagtgc gacttttgat acagtggcta aaaccatttt
    51481 tctatttgtc tgctttccat aaaaactagc ccgcagactt gctcctacca caccaccaaa
    51541 accggcgaga ttgttgatcg tgtttgttac ccacgcagca agaagaaact cttttctttc
    51601 catttttggt tttccttgtt tttctaagac tctgatcgtc acccaatcat acaacaacat
    51661 cggagtgaca cccagcagtc cagccagaat catgagcagg atcgtcatgc ggctttgttg
    51721 tcccatcctt tgccaaatat cttgccaact catgccatta gcaatatttg cgacttgatt
    51781 tgccacaaag actaacacgg agccgaaaaa aatcagtttt aaaatcagac tatgttcctt
    51841 tatccattgc agcatcaagt atcgtttatt tttcacattt tcccattcct aacttgtata
    51901 cttttgtgtc ataccccaat gttatcttat ttattttaat ttttccactt tagacctgtt
    51961 ctttgcttgc gatgatagaa aaagaaagat atcctaatgc taaaggagag gataacatga
    52021 aagtaggatt tatcggcaca gggaatatgg cacaagctat tattgatggt ttattaaaaa
    52081 aaggaacagt ctcgaaagaa gaggtgttcg tttccagtgg acattttgaa aatgcacaaa
    52141 aatttgctgc tgaaactggc gtccatgcat gcaaaagtaa tcaggaagtt gcatcacaat
    52201 cagatatcgt tcttttagcc gtgaaaccaa aaatcattga aaaagtattg gaagaactaa
    52261 gagaatatag ccagaaagtc ttattcattt ctattgcttc aggaaaatca ttaatagatt
    52321 tagaaacagc gcttgaagat cctcaggcct ccatcgtacg tgtgatgccg aatgtcaatg
    52381 tcagcgtagg agcaggcgtt tctgcgattt gcaaaaatga agctgccacg gaggaacagc
    52441 ttcagcaagc tacgatgatt tttgaagcaa tcggcagcgt ctatccttta gcagaatcag
    52501 atttcagtac attcattggc cttgctggca gctcacccgc attcgtttac atgctgatcg
    52561 atgcgatggg acggactggt gtactgaatg gtttgtccaa acagacagcc actgagattg
    52621 ctgcaaaagc aattatgggt tcttgtcaaa aattcctttc aagtgataaa aatccatggg
    52681 aacttgtaga ccaagtttct tctcctggcg gtacaactgt tgcaggtgta gttgccttag
    52741 aagaagcagg attcatccca gcaatcatca aaggaatcaa tacgacgatc gagaaagatc
    52801 ttgagatgtt gaataaaaaa taatgatagc aatgtttttt cgtgccttac aaaaaaagaa
    52861 gagctgtggc ttttgccgcg gctcttcttt tgtatttttt attatttagc ataatcagtt
    52921 gcacgagttt cccgaataac gattactttg atgtgtccag gataatccag ttcttcttcg
    52981 attcgtttac gaatatcacg tactaaacga acagcgtcta aatccgagat ttcttctggc
    53041 ttgaccatta cccgaacttc gcgtccagct tgaactgcgt aacttgattc tacaccggcg
    53101 aagctgttcg aaatattttc cagacttctt aaacgacgga tgtagttttc taatgattca
    53161 cttcgcgcac caggtcttgc tgctgagagc gcatctgctg cagctacaag gacagagata
    53221 acagaagtag cttccacatc tccatgatga gaagcgatcg cgttgattac tactgggttt
    53281 tctttgtact tggcagctaa ttccgcaccg atctcaacgt gagatccttc aatctcatgg
    53341 tcaagtgctt tcccaatatc atggagcaac ccggcacgtt ttgctaattg gatatcttcg
    53401 ccaagctctg ctgctaagat accagctaat tttgcaacct cgaccgaatg cttcaagacg
    53461 ttttgtccat aactagttct gaatcgcaga cgtccgatga tcttgattag atctggatga
    53521 agtgtatgag caccgacttc aaacgctgcc tcttccccgt attcgcgaat acgttcatcc
    53581 atttctttac gagatttctc gaccatttct tcgatacgcg ctggatggat tcgtccatct
    53641 tgtatcagtt tttccaaggt catccgtgcg atttctcttc ggattggatc gaatccggaa
    53701 agaacaactg cttccggtgt atcatcgatg attaaatcga taccagtcag cgtctcaagc
    53761 gttcgaatat tacgaccttc tcgtccaatg atacgacctt tcatttcatc attcggaagg
    53821 gtaacgactg aaacagtcgt ttctgaaact tgatctgctg cgcagcgttg aatcgctaat
    53881 gataataagt ttttcgcttt gcgatctgct tcttctttgg cacgctgttc agattctttt
    53941 accattagag ttaactcatg attcagctct tcttcagtcg atttcatgat gacatctcgt
    54001 gcgtcctctt ttgataaacc agcaattttt tctagttctt tttgttgatc ttcaatcatt
    54061 ttttcaacgt ctttttctcg ctcttcaatt aattgctgtt ttgaactaag tgcttcttct
    54121 ttactttcaa gtgagttctc gcgtttttct aacgagtcat ctttacgatt cagcgtttgt
    54181 tcgcgttgaa tcaaacgatt ctcttgagat tttaattcta acttactttc tctcagctca
    54241 ctttcaactt cagaacggta tttctgattt tcttccttag cttctaacaa tgcttctttt
    54301 tttagagttt ctgcttcttt ttttgcacta tcgatgattc cagcagctga tgattgtgca
    54361 ccagctattt ctttctcgtg acgtgacttt gcgaccataa accctaaacc aagaccgaca
    54421 attaaaccga tgatagcgag gagaatgttg aataccataa aatccacctc catactatct
    54481 ttttcatttt tatcatctat acagcttcta tgataaatta ttctaaactc gtcaatatgc
    54541 atcttcgcat atatgtgtac aaaaaacatg cacaacttta ttctaatgtt tgtaccccgt
    54601 agtgtcaact ttaaataaac aaagaaacgg ctttctactt attcattaaa cgatggtttt
    54661 taaaataaat gacactagtt tcatataggt aaacctttaa attgagaaac ttttctatta
    54721 aattattctt tgtagatgaa cagatatttt tattttatgt acccactcgt ataattaaat
    54781 aactcacgat ttctttattc aataaaaaaa cggcaggtca aggagagtac ctactcttca
    54841 gaccagccgt ttttgtttat cataattttt attcttctac taaaggcaat tcttcttgtc
    54901 ctttttcatc ttctgggaca gcgacttctt caccaatacc ataagctgct ctaactagag
    54961 cagaaacttc tgccatcatt tcaggatgat tagccatata gattttagcg ttttcccgtc
    55021 cttgaccaat ccgatcttct ttataagaat accaagcacc gcttttatca acaatgtctt
    55081 tttctactgc catgtctaga agttctcctt cttgtgagat ccctaaacca tacatgacat
    55141 ctacttcagc gattttgaaa ggaggtgcta ctttgttttt tacgactttg atctttgtac
    55201 ggttaccaac aatatctgta ccttgtttca attgttctgc acgacgtact tccaaacgga
    55261 tcgttgcgta gaatttcaat gcgcgtcctc caggagtgat ttctggattc ccaaacatca
    55321 cgcctacttt ttcacggatt tggttgatga aaatagcgat tgtttttgtt ttgttgatcg
    55381 aaccagagag tttacgcaat gcttgagaca tcaaacgtgc ttgtaacccg acatgtgagt
    55441 cacccatttc gccgtcgatt tctgctcgtg gaactaaagc agcaactgaa tcgactacta
    55501 cgatatctac agccccactt gatactaaag catcagcgat ctctagacct tgttctcctg
    55561 tgtcaggctg tgaaagaagt agttcatcga tattcacacc taatttttgt gcatattgcg
    55621 gatctaacgc atgctcagca tcaatgaaag cggccgttcc gccatttttt tgtacttctg
    55681 caatagcgtg tagtgcaact gttgttttac ctgaactttc aggtccatat acttcgatga
    55741 tacgtccacg ggggtaaccg ccgacaccta aagcgacatc taacgcaaga gagccacttg
    55801 gaattgtaga gatttgttgg tcgatttttt cgcccaattt cataatagag cctttaccat
    55861 agctcttttc aatttttttc agtgcagcat ctaaggctgc tttacgatca tctgccaata
    55921 cgataatcct ccttatattt acaaatcaga gcaacatttc attatcctta tcatatcttt
    55981 tttagtgaaa aaaagcaaga aaaaaccgaa caaatattcg catttatcat tttcttaaaa
    56041 gctgttttct caaaagatca aatccttgca taaccgcatt atatctgata gcagcacgat
    56101 ctctggcaaa gtgatatttt tccgaaacca cgccgtcttt agatgaaata gcgatccata
    56161 ctgttcctgc agattgacct tctaacgtgt ccggtccagc cactccggtg aaagctacac
    56221 tgtaatctgt tcttgctatc tcacgtgctt gaatggccat ctgctccaca cattctttgc
    56281 tgactgttcc atattctgct aatagctccg gatcaatctc cagaaaacca gcttttgttt
    56341 ggagcgagta ggtaacgaat ccgcctggaa atacttcaga tacaccagca atgtcgccta
    56401 atgcggcttg gaaggcgccg gctgtcaaac tttccgctgc tgttacagtc tgcttgtttt
    56461 ctttcagtag ttccactacg acttttgcta gcgaattatc atcgccgtat ccataaaaat
    56521 attcccctac tcgttcttgg attttttctt ctaatgctaa aagagcttga ttccctgcct
    56581 gaacatcatt tgttttcact gtcagtcgta atgtcacttc atttggtttg gcatatggtg
    56641 cgatcgttgg attgatctgt gtctcaatca gatcttttag ctcagtgacc agtcgcgatt
    56701 caccaatccc ataaaagcgc aaaactttag aaattagttt ttcttcagaa ggaaaacgtt
    56761 cctctagtaa aggacgtact tgttccacaa acattggttt caactcatta ggcggaccgg
    56821 gaagaaggat atacgcatgt gtatctgttt gataaaaagt ccctaaagca agccctgtac
    56881 gatttggtag aggaaccccg ccttcgataa tctgggactg ttgcagattg tttttggtca
    56941 tttttcgatg ggttgtttca aaatatgcca ataatttttt gtatccttcc gtattttgta
    57001 tgagagactt accgaggtga gccgcagttg cttcttttgt caggtcatct tctgtcggtc
    57061 ctaatccgcc acataagagt atgagttcac ttcttgtttc agctagagca atcaactctt
    57121 ctaaacgctg ctgattatct cctacaactg tttgataata aacttcgata cctagatcag
    57181 ctagctgttc tgacaagaaa gtggcattcg tattcacgac ttgtcctagt aaaagttctg
    57241 ttccgacagc tattatttct gctttcatta cttcccgctc ccttatatag tatacgctgt
    57301 atttttatat ttttttattt aaaaagaggg caaggagctg tgacaaaaat cttgtcacag
    57361 ctccttgttc caaataaaca gtgttctcct gccaaaacct cggtcataag tcacaatata
    57421 cataaaacat gaaaagctgt tttttgaaat tatcccttgt gactcagagt tagacggcag
    57481 gctcacaacc tctccactaa acgatactta cattgatcct ttgaacacac cgctgttttt
    57541 gatgaagtaa tccacacctg aatagatagt aaagatcaga caagcataaa gcatgatttg
    57601 atctactgga ataccgatca ggctgaatgg gatattattg ataaacagca agataatagc
    57661 aaccatttgt gtagccgtct tgacttttcc cggccaagct gcagccatga cttctccatg
    57721 ttcgacaagt agtaaacgca agcctgtgac ggctaattca cgacagacaa tgatcgctac
    57781 tacccaacca ggggcctttc cttggtcaac caacatgata aaagcagtca taactagcat
    57841 tttatctgcc aacgggtccg caaacttgcc aaaattagtg acaaggcctc gcgcacgggc
    57901 aatttttcca tctaaccagt ctgtgatact agctaaagca aaaataactg cacctactag
    57961 ctgtgtgaca gccagtgtgg tgtctcctac gttaaattgt ccccaatcta aaggtaaacc
    58021 tacaaccaaa atgaaaatcg ggatcataca aattctaagt acagttaatt tatttggtaa
    58081 attcacacta tcgactcctt tcttccctat tctacgtgac tatcagcgaa gtttccaccg
    58141 atattattta cttttccaaa tgaatttatt caaaggagta tttttattga aattgatctt
    58201 tttgaattaa cgaagaagga ccgcgacaca gatttgtcac gatccttctt ttttatacct
    58261 acaagtaaac agatgcgtaa actaatttat acgatcattc tgcgtattga agagtaaggt
    58321 tcacattttt tggactctga ttgttttccc ctggattaac tggtacggat tctccattcg
    58381 ctttgatcgt tgcattgctt gctgcaccaa tcgtgatggt cgcatttgtt gctgtttccg
    58441 gtaacacggt actctgtgtt tcatttgctg ccagtgtccc ttgataaaca taggcattgt
    58501 ctactagcac acctaaccat acacgattga cagcagtaaa ttcaagggtt acaggcttgg
    58561 tagcgtctgt gatatttact gcgatggcac tgccagaatc ttctcctgta gctattgcca
    58621 ttttcttttc ttcacttgat tttgttgttg attctactgt agaagactct ttggtctgcg
    58681 tgctttgctc tgaggaagag gactcctcga ctgatccttc tactttaagc gaagaagctg
    58741 tgtctccgat tattggatca gcttgacgat cttgccaagt catatagccc acgacaacga
    58801 caatcgccaa agcaaccaat cctaataaaa tgacaggaag acttgtccaa aagcgacttg
    58861 gatgcgattc ttcctgatgc attgcttttc tagaagtact tactgtttca ggctgcggac
    58921 gttcagggac cgatcctgtc aatttgcttt tcccatcaaa aacatcgact aaatggtctc
    58981 catcttctcc tacagcttca gcgtattgcc gaataaatgc ccgcacgtaa aatttaccag
    59041 gcaattggtc aaaatcatca ttgtcgattg cttccaaata acgtttttga atttttgtaa
    59101 tctgctgtaa ttcatctaaa gatatttttt tattcaggcg tgcttgccgt aatttttctc
    59161 cgatggtttc aaaagccact cgttcatctt ctccagtctg tttgattctc cgaagcatct
    59221 gctgttgcaa cgaaagacgt tatgtttggg tgctgtgaca aaagtcatgt cacagctcct
    59281 tttttccgaa taaacattgt tctcctgcca actcctcggt cacaagtcac aatatccaat
    59341 aaatataaga agctgttttt tgaacttgtt ccttgtgact cggagttaaa cggcaggctc
    59401 acgacctctt tcttaaacgg tgttcaatca gctgttcctc ggtattagct caaaaaccgg
    59461 caaaatataa ggagatattt ttccgtatgt ttctctaata ctcaggtcaa aacgctgatt
    59521 tcacaacctc ttccttaaac ggtgttcaaa agcttgcatc tgcggtaata agtccaacca
    59581 agttaaaaac attaagaagc tgtttttact tggctgttct tattacttga tgcagggcag
    59641 ctttttcaca acctcttggt acacggtgtt ccagaagtag cttcttcggc aataagccga
    59701 aatatcacaa aaatttgaga agcaattttc gtaaattcct tcttatttct tgaagctgac
    59761 cacttctgtc acaacctctt tgattacttc gtttgatttt attttaatat aagttgatta
    59821 aaagagagca tgatttgttt tattttctca ctaatttttc caacacaact aagaatacca
    59881 taaaagctga ttttggagga ggttcctact acaagtctaa aacgaaattt aagacttttc
    59941 ctcataaaat actggtttat tttggtctca tgtaaaactc gctgaaggcc tcttcactta
    60001 taaatgcttc cccagctgcc agtacgtctt tcatttggat cgaatcgatg atttcaggaa
    60061 gatcaaataa agttcgatcg ccaaataaac tttgcgtaaa ttgattggca atatattcga
    60121 tcgaatttaa tgattggaaa tattgtccaa gcattttctt cttcagcaaa tcgagatttg
    60181 tttctgacac ttccggatca tcagcgaatc caagaatgat ttctttgact ttttctgctg
    60241 ctttctctgg ttcgtccgta tctccgctga agtccgcaaa gtgaaattca cgatccaagc
    60301 tgaattcaaa accaaagctg tcatcgataa ttccttgatt gtacatcgct agatagtttc
    60361 gagaagtatt gcctaaaatc atttggaaca acagattgat tgctgtttta tagcgtaaca
    60421 gttctgttcc ttcttgcggc aatgtgtcta atcctttaat accaagtaca aatttatctc
    60481 gtgtaatggc tgcttccaat gcactttgtt tgatgatttc ttttgtgttt tcagggaaat
    60541 aacgtacaat ttcttgcttt ggagggaaat tttttgcttc ttggttttcc cgaataagtt
    60601 tcatcaattt ctcaggctcc atcttaccaa ctacaaataa aaccatattg cttggctgat
    60661 aaaacgtccg atagcacgta tacaagtctt gagctgtgat tttgtcgata ctttcaaccg
    60721 tgcccgcaat atcgatatgc aatgggtgtg taggatacaa gttatttagt atgccaaaaa
    60781 acatccgcca gttagggtcg tcttcataca tttggatttc ctgaccgatg attccttttt
    60841 ccttgtttac agtttcttct gtgaagtaag gtgcttgtac aaaatcgatc agagtcgtca
    60901 gattcttttc tacctgatcc gtcgtagaaa acagatagct ggttttcgta aaacttgtga
    60961 aagcattggc ggaagctcct tgtttcccaa ataattgaaa gacatcacca tcttcttttt
    61021 caaacaactt atgttctaaa aaatgggcaa ttccatctgg aactttcacc ttttcttttt
    61081 caccataggg aataaattcg ttatcgatcg aaccataatt cgtactgaac aaaccatatg
    61141 tcttgtgata gtcattttta ggcaacaaat acacggtcaa accgtttggc agtacttcat
    61201 gataaagtgt ttcattgatt tgctcatatt ctgttttatt catttgcatt tcctcctgct
    61261 aaaaagaaga ttgcctgcaa ttcgatttgc tcagctactt gttgtatctc tttgatcgtt
    61321 acgctctcca tacgtttcaa ccattcttca tcagataatt ttgtttccgg cagccagctg
    61381 tccaaaaaag aagcttcgat tgccgcttgc ggagagtcta gagaaagcag atattggttt
    61441 ctcagcattg cttttgtctg agacagttcc aactctgtta tttcaccatt tcgcaaactt
    61501 tctaattgct cgtgaatcag atgaagtact tgatttcggt tcttttcatc gataccggtt
    61561 tggacactca tgaatcctcg gaacgtatcc acgctactag aggcataata agctagactt
    61621 tctttctctc tgacattcat gaaaagttta gagtgaggaa aaccgccaaa taaaccgtta
    61681 aagaccatta gcgcaaaacg ttctggttca tcgtaataca catttgtctg ataagctagg
    61741 ttcagctttg cttgaacgat tggctcacgt acttgtcgtt ccttcacgat attcacttga
    61801 ggttgggtat aaaacagatc tggataaact gtttcccgcg actcaaatgg taattgccca
    61861 actgcttcag ctgctttgtc cggatcaaca tcgccgacaa caaaaatgtc gatttgatca
    61921 tttgccatca tttcttgata gtaggacgct agcgaactag cagtgatctt gtccaaatct
    61981 tcgactacgc ccaaccctgg gatcttttgt tccgggctgt tttcaaaata aagttcttgc
    62041 aaggcatagg aagcaaaagt ttgtttatct tccttcaaac tttccaaata tagacgcaga
    62101 ttgttctttt ccaagtcaaa cgtcaactga tcaaactgat gatttttaat atttggataa
    62161 aataaaacct cttttaaaaa atctaccgct tgagaaaaaa ggtttggatc attgacatat
    62221 tcaccattca caaaactgat ccctgcgttt agccaatgta aatttccttt ttttctaact
    62281 gataatccaa agcttgcgcc ataaagctct gcaagttgcg cacttaactt tgtctgatcc
    62341 gaataattca aactatttgt ttccaacatg ctgctgagaa gtgaacgttt cgtgatgact
    62401 tcttgttgca gacgcgcagt aaaacggata aaaatccgga tcgttttgta ttgttttgtt
    62461 ggtaagacat gaagattcac gccttctgcc aattgataca ccatatttat atgctccttt
    62521 tcacagttga ttgcccgctt actcttttaa tgggtcaacc tgcctttttt acttattcct
    62581 ctgcttgaaa tttataagtt caggcaattc gccgtttttt ctaaatgatg ttacacttta
    62641 tagtatagca tagatgtttt ttatcaaaag aaacagacgg ctaaaagcgt atattgtttt
    62701 tttatttttt atttttcgat atactaaaga caaataaaag aagggagggt cattcatgat
    62761 caaagaattc aaagagttca tcatgcgtgg agatgtcttg gatcttgctg tcggcgttgt
    62821 tattggtagt gcgtttacaa gcatcgtaaa ccaagtggtg caaggcttga tcacaccact
    62881 aataggttgg tttgtcgcaa tgatcacagg tacttctgat ttagaaggag cactttcaat
    62941 tcttgactgg tcgccgacaa aaggcgtcac atttgcgttt ggtaacgtta ttagtgctat
    63001 cattacattt ctgatcactg gttttgtctt attcttagtt gtcaaagcgg caaaccgtgc
    63061 gaagaattta cgctccaaag aagagcaagt ggaagtggaa gaaactccta ctgcagaaga
    63121 ttatcttagt gacatacgcg atcttcttgc caaacaagtg gcagaagaca atacacatcc
    63181 agtagagcaa acaaatcttt ccgatacttc taaataataa agaatctaaa aaaaagaaag
    63241 ccgattttca gtgtcggctt tcttttttta gattcttaac ttgctacttg ttcctttttc
    63301 atgtcgatat gtggaatatc atcttccaaa tacacatctg agattgcctt gaatccaaac
    63361 gactcataaa aagcttgtag gtattcttgt gcgccgatca cgattggttt ttctggaaaa
    63421 tttttctgga tcatgttaat cgtttcttga acaatctttc ttcctaaacc atctttacgg
    63481 ttgtcctgat gaacaaggac acgtccaaaa tggatactct ccgcttcttc atatactcgc
    63541 gtgtaggcaa ggatcttccc atcagtatct tgaaaaaacg tatgccaagc ttctccatca
    63601 atgtcatcaa cttcttgata aggacactgt tgttccacaa caaaaactgc gattcgttct
    63661 ttgacgatcg cgtaaaactc tgctgttgac aattcttcaa atttctttac ctgatattcc
    63721 actaagcaaa cgccccctgc ttttttattc ttagcatacc gattcgcaca caaaaaagca
    63781 aacgaacctt acgaatctca ggctcgtttg ctttttaaac tgcttcagtt aaaaattcga
    63841 tctcaactgt ccgagtaagt acatctgaat agctgtaaga tactcgttca aaagaatttt
    63901 cttcaggatc caaatcaaca acgaatactg aaggataggt ttctgttaaa acgccgcggc
    63961 gttctgtttg acgctttctt cctgtctgag caaccaacat gattgggctg ccgatacgac
    64021 cttctaaatc tttcttgatt gaagctaaag ttgttggcat acagttcacc tctactatca
    64081 actataccac atatttttaa aattttcaag tttaccataa aaaaaaaaga aatgcaataa
    64141 aaataaataa gaagctcatt aagatttttc taaattattc ctcattataa aatttcaagt
    64201 gtttttttat tttgagtttt actctcccgt aagcgctcat tacttgcttg tgatccattg
    64261 ctaaacattc agccatgtct acaaaatcaa cgccttgcat ataataatga aaaattttag
    64321 cttctatttt tgaaaactgg atttcgcaat tttcgattgc ttcttttaat aaaagatgct
    64381 gaattgctaa aggttcttgc aatgtgatct ctgctaaaaa gtctggtcct tctgtctcta
    64441 ttttttggtc gattgagact tcatcttttt gatcacgacg cttcagcgcg ttctgcttcc
    64501 tcaacagaga atagatttga ttgcttataa cgcttttata ataacttcca aagctaacac
    64561 ctttgctttt ttcgtaagtt ctcaatgcct gatataaagc gatccttgct tcttgttccc
    64621 aatcctcaga aacataattt cttaaataat aggtgttttt caaacgtagt aaaagtggaa
    64681 aatatttttg atacagggca gcaaatgctt cttcgtcacc attacgtgca gcgcgcaata
    64741 actcctcaga cataaaataa ttcctccata aattcgttta tggaggaatc ttatcatcaa
    64801 tgattcatcg aactgattag aacgatatgt tatttatttt ttctcattaa atcgtccaat
    64861 ttttgggaca atttttttaa ctgctcttca ttccatggag aattacgtcg gaaatcaata
    64921 aaatgcatat ctttattttc ttgggctatt gttttttccg ttttttttat cgtcttatat
    64981 aattcataag cagaagttcg taatgctcct tgggaaaaga tcatccactg ttctgctaaa
    65041 tcgcttgtcg ccactgtcac ttgtgtcaaa cggtcattca gctgaccagc gattctctcg
    65101 atataactgt cagccgtttc gtcttctttc gtaaaaacaa catccaactg gtatttctta
    65161 taattttgtt ggatgcccgg aactagttgg gcatcgaaga caacgatgac ttccattcct
    65221 tcatatttcg cataatttga taaacgatgt aaaagagctt ctcttgcatc cgccattttg
    65281 tcctgctttt taagctggac aagttccggc cacgaaccaa tcatgttgta gccgtctaca
    65341 atcaacagct gttttttcat ggtctactcc ccctgacgtt gggaaaaagc ctgatacata
    65401 agcaaaccag cagcaacact ggcatttaag ctctggacgt gcccggtcat cgggatcgtc
    65461 agcaattcat ccatttcttt tttcaatcct ggactcattc ctttgccttc attaccaatg
    65521 actaaagcaa ttgatccttt ggcattccat ttttggaagt ttgtgccggt catatctgtg
    65581 ccaaataccc aaaatcctgt ttctttgagc tgttgcacag cttgtaccaa attcgtcacg
    65641 cgtgcaacag ggacatgttc gactgcccca gtggaagtct ttgttacgat aggtgtgatt
    65701 ccaactgctc ggtgttttgg tatgatcaca ccatcgacgc cacttgcgtc tgctgtccgt
    65761 aaaatcgacc caaaattgtg tggatcttcg atgctatcca aaatcaaaaa gaaggggtct
    65821 tcttttttct cttttgttcg ttcaatcagt tggtcaaggg ataaatattc ataaggtgta
    65881 atcgcaagaa cgactccttg atgcacgccg tgatcgctca aagtttctaa tttttgttta
    65941 ggaacccatt tgactggcac agcttgttca cgtgcgattt ctttcagttc ttctactttt
    66001 tctcctcgtg aatcttcttg aagaaagagt ttattccctc gcccttgttg tagcgcttct
    66061 actgcggcat ggtgcccaaa gacgaaatta tcttctaatg cttcttcatt ttgatcagaa
    66121 actgactgat ttcgtccagt ttgcttttta tttgaatatc tattttgttt tttgtcttca
    66181 aatccacgtt tcgattgatg tttgtttgtt ctttctttac ccattttttt caccaacttt
    66241 ctgaatacac cagcggatca attcttccat ccgctcggtt tgttttgtca aatgcagata
    66301 gcccatgagc gcttcgaacc ctgttgcgac ccgatacgtc gtaacatcag cattttttgc
    66361 gctcgtatgg cttttagcat ttcttcctct gcgataataa agttcttctt cttctgtcaa
    66421 aagatgttct tccagcattt gctgaatcaa aaaggcttgt gcattcgcag aaacataatt
    66481 cgttgccgca cgatgcagct tattgggttt tgtttctcct tgatcaatca ggtggtcacg
    66541 aacatagatt tcgtaaatcg catctccaac ataagccaag gctaaaccgt tcaattgtgt
    66601 gtaatctctc atgcttctct tctccatcgg gttccttgcg gtgtatcttc taataagatc
    66661 cctttttctt tcaacaaatc acgaatttcg tcgcttcgga caaaatcacg attgcttctt
    66721 gcttgatttc gttcttcgat caattgttca atctcttcat ctaataactc ttctgctgta
    66781 aataaaatac cgaagatcga aagccattgc gagaataaag cttctccttg tgccaagaca
    66841 gcggcagaaa ctttcggctg gtctagataa gtgttcagcc acttagcaag ctcatagacc
    66901 actgtgatcc catttgctgc attaaaatca tcgtccattt cttcgatgaa tcgctgttct
    66961 aaattttcta acaaagcgat tttatccgca tcatctgcca aattctctga ggcagtttct
    67021 tggcggaatt tcaaattgtc aaacgtattc ttcagtcgtt gtaaattggt ggctgcttct
    67081 ttcatcgtcg tttcactgta gcggatcgga cgacggtatt gtgtggtcgc catgaagaaa
    67141 cgcaagactt gtggatcaac gtttttgatc atttcatgta cagtgatgaa attccctaga
    67201 gatttactca ttttttcatc gtcttcgcca atcgttacat agccattgtg catccagtaa
    67261 ttagcgaatt ttttccctgt tttcgcttca ctttgtgcga tttcattttc atgatgagga
    67321 aattctaaat cttgcccgcc accatggata tcgatcgttt ctcctaaatg ttttgttgcc
    67381 ataacggaac attcaatatg ccaaccggga cgtcctttgc cccatggtga atcccaagaa
    67441 atctcgccag gttttgcttg tttccataaa gcgaaatcta atgggtcttc tttttgctgc
    67501 tgttcttccc ctgtacgctg acttgctccg acttccagtt catcgatgga ttggtcactt
    67561 aatttcccat aattttcaaa tttacgggta cggtaataca catcacctga agattcataa
    67621 gcatagcctt tttcaaccaa cacttgaata aatgctaaaa tatctggcat atgatccatg
    67681 actcttggat gcagtgtagc aggttcgaca ttcaacgctt tcgtatcttc ttcaaatgct
    67741 cggatgaaac gatcagcaac ttctggtgct gtaataccta attcgtttgc tgtacggatg
    67801 attttatcgt ctacatccgt gaaattcgat acgaaatcga ctttatagcc tcgatattca
    67861 aaatagcgtc gaattgtatc gaaagcaatg gtgctacgag catttccgat atggatataa
    67921 ttatacacag tcggtccgca gacatacata ccgacatgtc ccttttgtat agggatgaat
    67981 tcttcttttt ttctggtcaa tgtattgtag atttttatca ttctgatctc ctcacttttt
    68041 ctcctttgat tcgaacgact tttgctggta ctcctaccgc tgtagcgtcg tctggaacat
    68101 ctttcaacac aacggcacct gcaccgattt tggcgttttt tccgatcgtt accggtccta
    68161 atatctgcgc attcgcagag agcatggctc cttgtttaac agttgggtgt cttttacccg
    68221 tttcttttcc tgtgccgcct aatgttacgc catggaataa aatcacatcg tcctcgattt
    68281 ctgcggtttc gccaatgacg attcccattc catggtcgat gaacacgcca gttccaatcg
    68341 ttgctcctgg atggatctca acaccagtta ggaaacgcca gaattgagca ttgatcttcg
    68401 ccaataaaaa gagccggtgc tgatataaaa aatgagagaa acgatgccaa aacaaggcat
    68461 gaaaaccagg gtatgtcaaa acgatctcag ctgtcgaacg tgcagccgga tcgtttctct
    68521 ttgctgcatc aatcgctcgg gtaaaccaac tcatgacttt ccactccttt cgtaagaatt
    68581 ttatgtagga aatcatcttc ttataaagaa aagaagcgcc ttttaaataa gtttccttat
    68641 caaaagacgc tttccgcgtg gttccacttt tcttcataat caatgattat ctctgacagc
    68701 taacgcctgt tatacgttct tggctacttt gtttcctata aaacagtttt tcctgttttc
    68761 tgtgtttcgc caagaccact cccagaggca tttcggcaac cattcattga taccttgcac
    68821 cacccggtat ctctcttgta atgatcagct gcttactttt ttctgttcat cgtgtttagt
    68881 tcagtacttg atttaaatgt gctaatgatt tttcacgacc aagcaattcg atcgtttcag
    68941 gcaattctgg accatgcatt tgtcctgata cagctacacg gattggcatg aaaagatttt
    69001 ttccttttac gccagtttct ttttgaacag ctttgatggc agctttgaca gttggagcat
    69061 ctactacttc catttgttct agctgtgctt tgaatgcgtg gagaacagta ggaactgttt
    69121 caccagcaag tacttctttt gctgcttcat tcaatacagg atgttcattg aaaaatagcg
    69181 tagacagatc aacgatttct gcagcatagc tcattttagg ttgatacagg ctaacgatct
    69241 tcttcaccca ttcgattgtc ttttcatctg ggttttctgc tacacgtcca tcagcgatta
    69301 gataaggcag acacatgtca gtaaatgcat ctagatccat tgctttgatg taatggttgt
    69361 ttacccattg cagttttttc gcatcgaaag ctgcgggtga tttacttagg cgttttggat
    69421 cgaacatttt gatcaattct tggtgttcga agatttcgtc ttcgcccaca ggtgaccagc
    69481 ccaataatgc aataaagttg aacattgcat ctggtagata gcctaattcg cgatattgtt
    69541 cgataaattg caagattgat tcgtcacgtt tgcttagttt tttacctgtt tcactgttga
    69601 tgatcaatgt catatgtccg aaggttggtg gtgtccagcc aaatgcttca tagatcatca
    69661 gttgttttgg tgtattggca atatggtcat ctccacgtaa aacgtgagtg attttcatca
    69721 aatgatcatc gaccgcaaca gcaaaattat aagttggcat accatcgcgt ttttggatga
    69781 caaagtcgcc accgatatta tcggattcga acgaaatagc gcctttcacc atatctgtaa
    69841 atgcgtattc tgtattgcga ggcacacgga aacgtacaac agattctaga ccttgtgctt
    69901 ctttttctgc ttgttcttca ggtgtcaagt tcgcacatgt tcctgcgtaa tgaggcattt
    69961 ctcctcttgc gcgttgtgct tcccgttctg cttctaattc ttcttctgtg caatagcatt
    70021 tgtatgctcg attgctagca agcagctgat caatcagcgg ttgatagatt tctttacgtt
    70081 cagattgacg gtaagggccg tattctccag gattttcagg agattcatcc caattcattc
    70141 ccaaccaagc aaggttttcc aactggcttt tttcgccatc ttcgatatta cgtttttggt
    70201 cagtatcctc aatacgaata ataaagtctc catcattgtg gcgggcaaac aaataattga
    70261 ataatgcagt tcttgcattt ccaatatgca ggtgcccagt tggacttggt gcatagcgta
    70321 cgcgtacttt cgtcattttc ttcctcttct ttcaaactag actttgacta acagcaaaaa
    70381 ttccgtcagt tacggcttct caaatatcat ttatttaaag ccactcagca caaattttac
    70441 cttttctaag gtaaatagta aagccaactg gttgaatttc cttaccagtt ggctgaattt
    70501 cttttttatt ttgattgttt agtctttttc tctgtctctt ttttattgtc gattcctcgt
    70561 cctgcatgtg caggtttagc aaagatcatg cgtccagcag ctgtttgaag ggcacttgtt
    70621 acaacgactt cgatacgttc attcatgtaa tgctgtccat cttctacgac aaccatcgtg
    70681 ccgtcatcaa ggtaggctac tccttgttgg cgttctgtcc cggcttttac tactagaaca
    70741 ttcatcgttt caccagggat gacgactggt tttacggcat ttgccaacgc attgatatta
    70801 agaacaggta cattttggaa ttcagagact ttattcaagt tatagtcatt ggttacaatg
    70861 acaccatcta aaagcttagc caatttgatc aatttgctgt ctacttcact gatatcttca
    70921 aagtctccat cgtacatttc gacagatata ccctcttctt tttgcaaggc atttaggata
    70981 tctaatccgc gacgaccacg cacacgtttc aaactatccc ctgagtccgc gatgtattgt
    71041 agttcgtaaa gaacaaaatt agggatcatc aaggttcctt ctaagaatcc agtctttgta
    71101 atatcataaa tccgaccgtc aatgatcacg cttgtatcca agattttata tttatggaaa
    71161 ttttctcctg attttcgttc taacagttga ctctcaactt gactagctgt ttcttgctcg
    71221 atttttttgc ttcgtgaacc aaaaactttc ttccattctt cgattcgtgt cgttcccatt
    71281 ctcaatccca gatagcctaa gatgatcatc agtaaaatcg gcaatacact attgacgaat
    71341 ggaatttcca tattgtaaag aggggtcgag atcacaacac ctaaagtcaa tccgatgatc
    71401 gcaccgatcg caccgaagag aagataagtc aagctgacct cacttagttt ttgttctact
    71461 tgtttgataa tagctgagat atgtttggcc aacagcaaag ataataaaaa gaaaataagt
    71521 gcgcctaaca ggctgttggt aaatgtatta ttcagccatc cgtttgactg ttgaccgatt
    71581 gcctgccaag ctgagggtaa aaatgaaata cccaagcttg cgccgacaat gatcatcagc
    71641 aaagtaatga cacgtttttg catcgttttt cctccgtttc gacaatcatt tattagttag
    71701 cggaacacac gcttcaacgt ttcagccaaa gttgctactc caactatctc gattcctttt
    71761 ggcgcttccc agttacctaa gttgttttta ggtaggtata cttttgtgaa gccgagtttt
    71821 tggacttctt tgactcgttg ttcaatatga ctgacacgtc tgatttctcc agtcagtcca
    71881 atttcaccga tgaaacattc cgttggctgt gttccttttt ctttatagct tgaagcaata
    71941 ctgacagcga tagctaaatc aatcgctggt tcatttaatt ttaccccacc tgcagctttt
    72001 aagtaagcat cttgattctg aagaagcaac ccagctcttt tttccagcac tgccatgatc
    72061 agagataccc gattgaaatc aagacctgtt gttgttcgtt tggcatttcc gaacatagta
    72121 ggtgtgacta atgcttggat ctctaccaaa attggacgtg tcccttccat tgccacaacg
    72181 atagcagagc cagttgcacc gtctaggcgt tcttctaaga aaatctgtga aggattagcg
    72241 acttcttcca atccatgctc atgcatttca aaaatcccaa tctcattcgt tgagccaaat
    72301 cggtttttta ctgctcgcaa aatccgaaaa ctatgatgtt gttcaccttc aaaatataac
    72361 accgtatcca ccatatgttc taacattctt gggccggcga tcgagccttc tttggtaaca
    72421 tgaccgacga taaaaatggc tatcccattt gttttagcgg ttttgagtag ttctgcagtg
    72481 gtttctctta cttggctgac acttcctgcc acacttgtga tgtctggctg cgtcatcgtt
    72541 tggatggaat caatgataac ataatcaggg gagatatgtt cgatggcacg actgatttcg
    72601 ttcatatctg tttcagcgta tagataaaat tctgtgttga tacttccgag gcgttccgcg
    72661 cgtaatttta tctgctctgc gctttcttcg ccggatacat ataagacttt tccgcctatg
    72721 gctgctaatt gctgagaaac ttgtaacagc aaagtggatt tcccgatacc tggatcgcca
    72781 ccaatcaaga ccatagaccc tggcaccaca cctccgccta acacacggtt cagttcttct
    72841 aattttgttt tgatccgagg ttctttttta gggacgacgt ctgcgatttt tgttggtttt
    72901 gctttttctc ctgtcaaact tgtccgagtt ctgcggtccg ttgtatcttg ttcgatttct
    72961 tcgaccatcg tattccattt tccgcaattt gggcagcgtc ccaagaactt aggcgacaca
    73021 tacccgcaat tttggcaaac aaattgtact tttgattttt ttgccattgt accgcctcct
    73081 tttatcctaa tcatgagaca ttaaatcaag cttaagtatc tttaacgttt tatttactgg
    73141 aagaaccaaa tccgccttga cgttgagcag tcgtttgatc ttgatctgct tttaaaaacg
    73201 gcaaaaagat cccttggccg atacgctcgc cttttttgat cactacatcc ttgaaaccaa
    73261 agttggtgaa ttggaacatg atatggcctt catttgcctc attattgtaa taatcactgt
    73321 caatcacacc tactccgttt gtcaacacta aaaaccgctt caacggattg cttgaacggt
    73381 tagcaagctg caaaaattca tcttctccca tgtaagcttt gattccagta ggcacgaggg
    73441 ttggtttcaa ttgtttttca ttttctgata catctttttt caatccatct gcgactgttt
    73501 ccaacccctt tttccagcct gctttccaga tacttggaat cacgacctct tctgctgctt
    73561 caaaatcata gccagcagca tgatgtgtag cgcgctgcgg tagattgata ttttgatctt
    73621 tatatttaga aataacttca aatcctcttt ttctcaccgg ttttcctccc taaataaact
    73681 actcctattc taacatatcc gaggctgtaa aagactttct ttacaaattc ttcaagaaat
    73741 tctgctatct taaagtaaac cattaattcg ttctttttcc aaacgactgc aagttgtaca
    73801 aaaaaactat tttttacatg actttactcg tcaaaacaag tagaatagaa cgaaattaat
    73861 gagtggatat ttgattgagg tgtcacatgc gtaaagaaat taaaaatgca aaacgaatta
    73921 ttgtcaaagt aggaacaagt acattgattt atcccaatgg aaatatcaat cttgcggcaa
    73981 tcgatcagtt agcttttgtc ctttctgatc ttagaaatcg gggaaaggaa gtgatcttag
    74041 tctcatcagg agctgtaggc gttggtatgc ataaattaca tttgtcagaa cgtccgactt
    74101 tgattcctga acagcaagct gcggcggctg ttggtcaagc cgaactgatg aatatctata
    74161 atcaaagatt cagtgtatat aatcaacaga cggcacagat cctcgtgaca agagacgtgg
    74221 tagattatcc tgaaagtcgt aaaaatgtga ggaatacatt ggaacagctt ttgattatgg
    74281 acacgatccc tatcgtgaac gaaaacgata ctgtagcagt ggatgaatta gatcatctga
    74341 caaaatttgg cgataatgat cagctgtcag ccatcatggc aaaacttgtc gcggcagacc
    74401 ttttgatcgt cctttctgat atcgacgggt tttattcaga taatccgact acaaatcccg
    74461 aggctcagat gtatcgctat atcgataaaa tcgatgaaaa gattctggca caagcaggtg
    74521 gtgctggtag cacatttgga acaggcggta tgcagagtaa attaaaagcc gcttctagga
    74581 tatttgaagc aaacagcgca atgattttag caaatggaaa aaatccggca attatttttg
    74641 atattcttgc aggaaaagaa gtaggtactt tatttaagga ggacttgaaa tgactgactt
    74701 gatgcaatta ggacaacagg caaaagatag cgcgcaacaa ctagcactta tgcctacaaa
    74761 ccgcaaaaat gagctacttg ttcaaatggc acaaaccatc aaaaagaatc aacaagcgat
    74821 cattgaagca aatcataaag acttagaaaa agcagtcgaa aacaatatca gtgaaacgat
    74881 gcaagaccgc ttgcagctga cacctgagcg aatcaatgcc atggctacag agatcgaaaa
    74941 aattgcttcg ttagacgatc ctattggaaa agtagatgag atgtggacga acaccgacgg
    75001 attgagaatc ggcaaaaagc gcgtgccact aggcgtgatc ggcatcattt acgaatctcg
    75061 tccaaatgtc acgacagatg cagctagtct agcattcaaa tcagggaatg ccgttatcct
    75121 tcgcggtggc aaagaagcat tcttctcgaa tcaattatta gtccaattat tgcagcaagt
    75181 actggtcagt gctggcgaat ctccttatgc catccagttt gtcgatgata cttctcatga
    75241 aactgcagga cagttgatgc gattgacaga atacttagat gtgctgattc ctcgaggcgg
    75301 agcaaaatta atccaacgag tcaaagaaca ggcaacggta cctatcattg aaacaggtac
    75361 aggaaataat cacgtatata tcgataaaga agcacagtta tctatggcag tgaacatcat
    75421 cgtcaatgca aaagcttccc gtccatctgt ctgtaacgcg gcagaaacat tgctgatcca
    75481 ttctgagatc gcaccttttt tcttgccagc tattgaaaaa gaactggtag aacacggggt
    75541 ctctttacgt gcggatgctc gtgcgttaga atatctagag acggctgctt tagcagaaga
    75601 cacagattgg gatactgaat atctagatta tatccttgct gtcaaagtgg tcgattcttt
    75661 ggatgaagca attgcacata tcaatcggca taatactaaa cattcagaaa cgatcgtgac
    75721 agatagttat gcggctagtc aacgtttctt aaatgaagtg gacgcggcgg ctgtctatgt
    75781 caatgcctcc actcgtttta cagacggatc ggtatttggt tttggtgcag agattggtat
    75841 ctctactcaa aagttgcatg cccgtggtcc aatggggtta aatgaattaa cttccacaaa
    75901 atatatcatt tatggcgatg gacaaatccg cgaataatgt ttcgtgtgaa acattattcc
    75961 cgccttttgc cttttttctg taaaggttct atccaactag aaaggtgtca atatgtttga
    76021 aattttaatg atttttttta tttaccttat ttctctgaat atcgctgctt ttctgggagt
    76081 cggtatttta tcccttttct ttcaattcaa aaaacgaagc atagggagtc aacgggaaag
    76141 atggtcgcag tattttgaca aaatcgggca aaaagggctg gttacacgct tgcacattag
    76201 ttacatggtt gcattatgct tattagcgac aatcaattac tattcttttt tcgatcattc
    76261 aatcgcttat acgattacgc tactgattgc tgggattttt cacttatctt ataaatatca
    76321 gctgaataaa aaccacttga atcggacttt ccgctaaaaa atatccgtac gataagtaga
    76381 gtgccttgaa gatcggcaaa aaactgccgg actttttaga ggctgtgaca aagattttgt
    76441 cacagcctct ttttccatat aaacggcgtt cgcctaccag ctgcctggta aatctgtttc
    76501 ctcttcgtta agaagcgaaa atccgtcaaa tgtttcgcca tcataataac gaagcagctc
    76561 atccatggac aagttcatcg ttttttcttt ttcttcttcc gctgattcag ccagtagctg
    76621 ttcccgatac acctgataca taccagctat tttttgctgc atcgcttttc tagtttcacc
    76681 ttccgcattg attattggca attctttgat atagccacga taaatcaacc gatcatctga
    76741 aaattcaatg ggtaattcaa cgagtgtcaa cccgacttcc tgaaaatatt cttccagcat
    76801 ctattatccc cccattcttt ttcttaaatg aattttagct tacctactga aaattttcca
    76861 gtttcattat tttgagaaaa aaagggaatg tgacaaaaat cgtgtcacat cccctttttc
    76921 cgaataaatg gtgttcttct gccaactcct caatcataag tcacaatatt caacaaacat
    76981 gagaaactgt tttttgaaat tgttccttgt gattcagagt taaacgacag tctcacaacc
    77041 tcttggtaaa cggcgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggtaaaata
    77101 tgagaagcta tttttccgtt ttgtttctct aatacttagg ataaaacgct gattttacaa
    77161 cctctgttaa acggtgttca atcagctgtc cttcggtatt agctcaaaaa acggcaaaat
    77221 atgaggaact atttttccgt ttgtttctct aatactcagg acaaaacgct gattttacaa
    77281 cctcctgata aacggtgttc aaaagcttgc atctgcggta ataagcccaa ccaaacgaaa
    77341 aatatgagaa gctatttttg tttggctgtt cttatgactt gatgcaggac agctttttca
    77401 caacctctga tttcttattc ttcttcattt gtagcaaaat aattacggtg ataaatgata
    77461 caacatacac tgataagaac aatggcacct gcaaaataaa acgcatagct ttcaccaata
    77521 tgttcaccaa tcacacctgc aataaatggt gctaaagctc caccgaaaaa gcggacaaaa
    77581 ctatagctgg acgaagcaac gtttcgttgc agacctggta tttccatagc aatcgtcgtc
    77641 aacagtgtat tgataattcc ttggaaaaat cctgctaaca caatgctgat cggtacaagg
    77701 actggcatag aagttccagc tcctaataac agcaaacaaa taatgaataa gacaaatgct
    77761 gctaaaattg tccgataagt cgtaaacttc cgctcaagag ccggtgcaac gaaaatcgaa
    77821 gcaatagcca gaagcacacc ccagccaaaa aatacaaaac cgacttctaa ttcagaatag
    77881 ccaatcaata aaaatggcgc atacgcaagt aaagtaaaga atccgaaatt atataacaat
    77941 gcgattagtc caactgaacg aagtttatga ttgcttaacg ctttgacacc ttcccaaagg
    78001 cctacttttc tctttggttt ctcagtttca ggtaaaagta ccgctagggc tataaaaccg
    78061 caaaacatca aaaatgctac cccaaaaaat ggataacgcc atgaaccact gcccaagacc
    78121 ccaccgacta gagggccaac agccattccg cagccaagtg ctgcttcata catcatgatt
    78181 gctttttctg tgttaccaac taaaatactg acgatcgttg ctaaagcagt agagacaaat
    78241 aaagcattcc cgacacccca gccggcacgt aacccaatca gcattccgat attcccagat
    78301 aatccgccaa gaccagcgaa tacaacgatg ataaacaatc ctgtcatcaa tgtttttttc
    78361 aaaccgatcc gactagataa gaaacctgta aatagcatca ccacaccagt gactaacatg
    78421 taactagtaa ataacagggt cgtttcagct ggcgtagcgt ttaactgtgc agcaatcgtt
    78481 tttaagattg ggtctactag acccactccc ataaaagcga tcatacaggt agctgctaca
    78541 actagcgctg aacttttccc ctttgtttct tcatttgttt gttcttgtat atctatttga
    78601 ttctccaata tttccatccc tttctctcta ttagcatcat acatgattta ttttacatgt
    78661 caatatgcac atgtaaaacg tggaaacttt actgtgatat aataaagaaa ataagacgag
    78721 aggtaggatt tttatgaata ctttagccta tatattatta tgcgtgcttt tatccaaacc
    78781 acgttctggg tatgaattga agcagctgat cacaattttt tgggaagccc atcacagtca
    78841 gatctataca acgcttgcca agttagacca gcagggttat gtgaccgttt tggacaaaga
    78901 cgagcactct caaaaaaaga tctaccactt gactgatgaa ggacgtgctc ttgtagagga
    78961 atggatcaaa gaggaaactc ccgctcctac gcaaaaagat gaatttctag caaaaatcta
    79021 tgctttttcg acgctcgata aaacaacagc tactggactg ctattcacta gagaacaaca
    79081 gctgaacaaa atcatgcaaa aaaatcaaaa aaaattgaat gacctctcgc tagacaagaa
    79141 acaagaattt ggacgttatg ttgtgatcca gcgcagaatt atgctttgtc agcaggaact
    79201 ccaatggtgt cagcaagtaa gagaacaaat gcagcagttt tttgagtaag ggataaacag
    79261 aacctttcta gaaatatcta ttgaagagca aattatcttc aataaaagag caatacagtt
    79321 ggaaaagaaa aagcgatccg aacgacaggc caaaacctat cgttcggatc actatttttc
    79381 tagaactgca ttcaagagac tatgacaaaa agcatgccaa ggcctttttt caaataacag
    79441 tgttccatca ttcattactt tgaattgctt ttagcacact tcttgaaagc gggccaacga
    79501 tgatcaactg caacggtaaa gcggcaatca cattcatttt ccaagcattc atataagcag
    79561 agaataattg atcaggtatt ccttgttcca tgatcacacc gaataaagac atacatgtca
    79621 ccattcctaa aaccatcaag catgaaatag ttaagatcat ttggaccttg ctttctggat
    79681 ttactggtag tttaaaggca atttttttcg ccagcacacc gatgatgaag acatccaaaa
    79741 tgaaagccac cacgaatcca ggcactagac caatcaacaa acttgataaa tgaagatctt
    79801 gatgcagcca taaattatac gcgctcatcc ccaagaccat caaaaaacac atcattgttg
    79861 taaaaattat tccttctttt ttatttgtag gcaatctgta ccactccttt aaatttgtca
    79921 cgaaaatcat cgtagcacat ttaaaaattt ctcgtaagta ctttttttat tttctgccct
    79981 tttatttcat aaacttcatc agccgtacgc tcgacaaagt aagaatcatg agaagtaaac
    80041 aaaatcgttc ccggatatat ttgcattagc ttctctaaag cttcgatcgt tgccatatca
    80101 ataaaattag tcggttcatc taacagcaac acattacttg gcttagtaaa aagcaaagca
    80161 atcgtcagac gggtcgcttc tcccccactt aatgtacata acggtttcgt caaagtttcc
    80221 tgagcaaacc ctaagtgatt cagcaaacta cggacaagcg attctgaaaa ctctgtttgt
    80281 ctcatgacaa aacgaatgat ggattcttca gatgtcattt gataaccctt ttgctgatat
    80341 acttgaaagc ttactttcgg agagagaagg attccttctc cttgtttgcg gatatgttct
    80401 aataaggttg tcttacctga accattttca ccgacaatcg cgattttttt acctaaagaa
    80461 aactgaaaat caccatttac cagtaatgtt ctcccacttc tttccaattg gacattttgt
    80521 cccatgatgg gatagcggct gtggagttca agagattttg gtactggaaa acggatttgt
    80581 ttcatctgct gcggcttggt tgtttctcct attttttgga gtcttctctc taatgttttc
    80641 gcattctttt ggatggcttt ttgtacactg tctttttgtt tagaggaaga caaccgatcc
    80701 agtctgattt gttgcttttt ctttttactg gacacttttt ccaacttttc cgcttgtttt
    80761 ttcttttctt ggatcgattt ttccaatcgt tttttctctt tcatgatcga ctctgcttct
    80821 cgtagctgag tctttttctc caattccttt tgagtgagat aggcactata attccccgaa
    80881 aactctcgga tttctccgtc ttttacttcc cagattttcg atgctagctc atctagaaaa
    80941 tagcgatcat gactaacaaa aaccagcgta ccataataat aacgaagctc ttcaatcagt
    81001 cgatcaatac tttttctatc tagatgggtt gttggctcgt ctagaagcat cccttgttca
    81061 taagaagaaa gaagacgtat caactcgatc tttcgttctt cccctccgct ccatttccgc
    81121 tctgaagttt cttccagtct taactggaaa tgactggcta attccaaatt taggtccttt
    81181 gcttcagcga gataggttaa ttgaggcagg tagttgaagg tgatctctgt ttggattttc
    81241 ccatgatcgg gaaaaagttc accggctatt agtttgagca aagtcgattt accgacacca
    81301 ttttctccta tgatccctat acgatcttgc tgatagacac gaagagaatc aatagatagt
    81361 tctagctggt tgccaaagct gacttgtaga tttgttatat ttactgctaa attttccata
    81421 tgttttacac tcctatttca tgtaaaacaa caattatcga tactaaaaaa gagcagaaaa
    81481 atctgtttgc aaaaaaactg catcacatct tttcctactc tgaacgaaat aactatgact
    81541 aaatcaccaa atctgtcata gctttcacct attctactgt cgttcgaaaa aatggacata
    81601 ctccatccat gaaattgttg tttatacatg tcttttgaat ttatttagtt gttattcaaa
    81661 agttcgaaac gtaatttcat cgatgcagtc atttttccga cctccttttt tattaggtta
    81721 ccacacacga ttcaaaaaaa gcaatagcta ctgaatagta actgaaaacc ttttattcga
    81781 acatagtgat cttttctggt gctaaacgaa cagactcgta accttcttct accgcttttc
    81841 ctattgataa aataataact ggaacataac ggtctttatc caaaccaaat gcctctgcca
    81901 actgatctgc ttcgaatcca ccaattggat ttgtttcata gccatgatca cgcgcaacta
    81961 acatgaattg catagccgct aagctagaat cgactttgac tacatcgttc atttcttctc
    82021 ttgaaaaact cttgtaataa ggaatgatcg cagctaattg ttgatctctg acttcttttg
    82081 gcatcttccc tgattcgtaa gcttggttgt agatttcttc gccatattcg taacattgca
    82141 tatcgccaaa aatcatgacc attgcagaag aagtatcatt ttgtctcgta ttgaaacgga
    82201 tcaaaggacg caagatttct tttgcttcct cgctttcagc gaccacaaag cgccatggtt
    82261 tcatattgac agaagaaggt gcagtcgtcg cttctttgat catttccagc atttcttcgt
    82321 gtgggatttt gtatgtttca tcatatacgc gaacggatct tctaccagtc ataattgttg
    82381 aaaaatcatt tttttgttcc attattatta acctctttcg tttctgttgt tatattgcaa
    82441 agttgggtat tcaatttatc taaaagtgtt agaaattgtt tcatttctac atcgctgaaa
    82501 gccggggata gcacggaacc tgtatcttta tggtccattt cacaatgctt gattttcttc
    82561 tgggcttttt ctgttagcgc tacatacact tcccggttgt tctgaggatt tctctgacgc
    82621 gaaacatagc catttgcttc caataccttc agatgacgag taactgctcc ttgatcaatc
    82681 tgcaactttt cttgcaaact gctttgtagc aatggatttt cttctttcag tctcatcatg
    82741 atctcgtatc tagtaagact tataccgata ttttcttcaa agatctggac gattttttga
    82801 tcggctaatt tgatttgata taaaaggtga ctaatctctt ctaaagacat aatttcctcc
    82861 tttctaataa ttgactcgtc aacgattgac ttgtcaataa tagaataaaa aaagaaaaaa
    82921 gtcaaagaat cagctgtgta ataaaaaaaa cgctctcaat agaaaagtat agagaaaaga
    82981 aattttttgt agaatgaacg gaaaaaatga taaagaaagc acaacgctaa aaccaagatt
    83041 attgattctt atcaaactat agaagatagc tcttctgcag tagataattt tacaccgcca
    83101 aaaatttccg aaatcgcctt ttgcaagaat cccgtcgaat attagaaatt ggatcagtat
    83161 gcttaaacag taataaaaaa gttatgacaa gatgttgtca caaccttttt tgagtaaacg
    83221 gtgttcaaaa acttgcatct gcggtaataa gttcaaccaa acaaaaaaca tgaaaatctg
    83281 tttttgtttg gctgttctta ctacttgatg cagggcagct ttttcactac ctctttttaa
    83341 taaccataaa atcctattcc ttcatatctc cagcccatcg gtgttagcca atctctttcc
    83401 ttagcactca aggtatagtg atgggtacca gttttttcat ttggattaaa tagtcgataa
    83461 attggcttgg aaccaccaga ataaaaactt agaccttcat aacgccaacc gatttttgtt
    83521 aaagagttga tctcagattg tgccattgtg taatgatggt ctccactgta aggattgtat
    83581 aaacggtaga caggttgtcc gctgtttggt gcttgccaag caatcccttc atattcccag
    83641 cctacagttg gcagataatt tttttctccg gcattcaatg tatataaatg ttctcctgta
    83701 tgacggttat acagacgata catcggagaa gttgaagttg gtgctgtatc atactgtgtc
    83761 agattatagg tttcgattac attgttcaat gtagaaccat aattaggcgc agtggcataa
    83821 cgacctgtta gccaagccgt agcatctttg taagagttcg tattgctttt ccatgcaccg
    83881 gaatagtagt aaacgcctgg ctgaaaagaa gtcgtcttca ataccgttac gttatcttgc
    83941 aatgattctt tgtacgaagg atattttcta aaagcggcat tcaccgtaat ccattgccca
    84001 ttcacatatt ccgaagtagg catcgtaaca gattggccat tatagcttcc tttaatccca
    84061 aatagattgt aattaggggc agaagccaaa gtactgttcc cccagccact ttcaacgatg
    84121 gcttgagcaa tcatgactga tgcatataag tcattggcac ttgccaaact ttgtgcagtt
    84181 gaggcaatac tatcaatgaa cgcttgttga gaattagcag atgagcgagc ataaaaagca
    84241 tagccagtat agttttcgaa ttcagacaaa tctgcttgat gggtatgtgt caatgtttgc
    84301 ccatctgtca attggttcgt gatttcttct gcattctctg gattgttctg agcttctgct
    84361 gatgtactgt cttcggtttg tgtttgttct gttgtttcag ttgttggttc ttcggttgaa
    84421 attgtatttt ctgagacagt agctgactgc aaagtacttt ctgtatcgtt ttcaattcct
    84481 gtttctgaag aactcgatgt ctgagttgcg acagtttctg tcgattcttc agctagcact
    84541 gttgtgttgg caactgctag atttcctaaa agaataacgc taaataataa attctttgct
    84601 ttcatctttt ttctcctttg aattatgaaa cttttcctaa ataacagtgt aactaaaaaa
    84661 gtaaaagcaa gtcaatgtcc atatttaaat aacatttgtt tctttttaat aaatttgctt
    84721 tttctgcata aaaagacgta aatgaagaat attcttctcc attcacgcct taaattgatg
    84781 gtcgtttgaa taactattta acaagtaatt ctctctggat attatccgtt atggattata
    84841 cttcctgtgt cgtcggacgg ataatcatgt tgctgacaga catccggtct ggcgtttcta
    84901 tcgcaaatgc tacagcagaa gctatatctt cagatgttaa tccccattct ttttgcgctt
    84961 catgaagttc aagagcagcc tttttgtcgc tgatggtttg atataattca gtttgaacag
    85021 ctcctggaga gatgatcgta gatttgatgt tattttgccg ttgctcttga cgtaatcctt
    85081 ccatgattgc acggacagca aattttgttc cgcaataaac ggcggaatca ggataaacaa
    85141 catgtcccgc cacagaatca gttgaaatga tatgcccaga tttttgtttt tccatgattg
    85201 gaagtacggc ggaaattcca tttaacacac ccatgatgtt gatatcaagc atattttgcc
    85261 attcatcacg gtgaccttca actaatggag cggttggcat gattcctgca ttattgtaca
    85321 atacatcgat tcgaccgaac ttttccatcg ctaaatcgat aactgcttgg acttgtgcaa
    85381 aatctcttac atctgctgtt tgaatatata gctcagcttc tggtaaagat tcttttattg
    85441 cttttaggcg atcttctcgg cgtgcagcta taactagttt agcccctttt tctgctaata
    85501 gcttcgttgt tgcttcaccg attccgcttg aagcgcccat gatgacaatc actttttctg
    85561 ataaatcatt cattctattc tcctcctgtt ttgaaaaatc tcttgcttat actcactatc
    85621 tcttatttca tcttactgtt caaataatcc gttcgacagg tttgacgatt ttggcaggga
    85681 ttcctgcaac gatggtattg tctgggacat ctttcgtaac agtgcattcg ctgcaacaat
    85741 ggcattctcg ccaatcgtta cgcctggcag aatagttgct cctgctccga tccaagcatt
    85801 tttcttgatg tggattggct ttactgacaa gcctcttcgt tcttttggat cggttagatg
    85861 gttcaccgtg atgaggttga cacgaggacc aatcaatact gaatcttcta aacagatgcc
    85921 acctaagtca acgaacatca cttgacgatt gataaagaca tttttcccta tactaatatg
    85981 tttgccgaaa tctgtataaa aaggcagcag aacagtcacc gtatcatcta ctttgtcact
    86041 aatgatattt tctagccgtt tgcggatatc tgctggttca tggtacccag tattcagcgc
    86101 tgctaaaggc tcttcgtttt ccgcttggat ctgatggatc acttcaaaga gcggatccgt
    86161 tactcgaatc tctttattga cgatttgttt ttcgatgagg cttgtcattt tgctccctcc
    86221 ttttatcctt actttataac ggacggctta tgatgtctaa tacttataaa acatacttaa
    86281 ctattccttt taactatact tagtggtatg cttttttaaa aacaggaggg aaaaaatgga
    86341 tattcgtata ctgacgtatt ttttagcagt agctagagaa aaaaatatca gccgagcagc
    86401 aaaatctctg catatcacgc aacctactct ctcaaaacaa ctgaaagatt tagaagaaga
    86461 acttggtgtg acacttttca ctcgaggctc ccgagagata cagttgacgg attctggtcg
    86521 atatctttat tcaaaaggaa aagaaatctt gtcgttggta gaaaaaacgg cgaacaattt
    86581 aacaaatgac gcaatcatct ctggagaaat ctacattggc ggaggcgaaa ctaaagcaat
    86641 ctctttcatt tctgatagcc ttcattcgtt gatcaaggcg catcctgata tccagtttca
    86701 tttatatagc gggaatgcag atgatatcat agacaaactg gatagcggat tacttgattt
    86761 tggactcgta atcgaaccga caaacaaaca aaaataccag tcatttcgac tgccagccca
    86821 agatcgttgg ggacttttga tacatcggaa tcatccttta gcaaagcaaa cagtcatcca
    86881 tccgcaggaa ctgaaaactg ttcctttgat catttcccgg caaacgtcag tggacagtca
    86941 actagctgaa tggctaggtt tcagcataga tcagttatcg atcgtcggaa catataactt
    87001 attgtacaat gcttctttga tggtcgataa cgggactttt ggtgcattag ctctagatgg
    87061 gatcatcaat acttatggga cggaaaaagt attcatccca ctcgatccgc cattacaagc
    87121 ttcactgaat ctcatttgga aaaaagacca gcctctttct cctgctgctc gtaaattttt
    87181 agatgtccta acggagaaat acagttaagt agagtttttt tccttcgctt tcagcttttt
    87241 aacaacgaat aagttaagca aaagatccaa tgattaaata acataagttt cttaatttgt
    87301 tccttccttt tatttctgat aattccagta aattactata attataaaaa aagaattgac
    87361 aaaacataac tgtctatata aaattgatta cgttaacaat aattcgtttc atgtgactag
    87421 atcatactag gcatgaaaaa tctctgagga acatggtttc cctaggaggt ttttcgtgtc
    87481 tatttttgtt tggccgcaat caactggtct agcaaacgtg aaaaccaaat cttcaaaaag
    87541 ggtgcgcgca tgaaaatcaa aaagatcttt aatcaaaatg cagtattggt agatgacggt
    87601 ggacaggaaa aagtagctat tggaaaaggg attggatttg acagaaaaag aaatgatttg
    87661 atttttgatc gtgatgtcga acggatattt gtcatggaac cagaaggtca aatcaagttg
    87721 caaaatttac ttaatcagat tgatgaacgc tttttatttg cagcagaaac gatcattgat
    87781 catgcggaaa ctgttttgat ggaaaagttg aatgaacatg tattgatcgc tttgaccgac
    87841 cacattgctt tttctgcgga gaacattaat aacggtatcg tcatccgcaa caaactttta
    87901 agagaaatcg aagtgcttta cagtgatgaa ttcagtattg ctcaatgggc agtagagtat
    87961 ctgacaaaag agcttgggat tccatatact tatgacgaag caggatatat cgcaatccat
    88021 attcacagcg gacgaagcgg tcggaacaat aaccatcgca gtatccgtga agtgacgatt
    88081 gtatctgaca tcatccattt agtggaatca gaattggaaa cagatatcca ttcagaacca
    88141 ttttcactga attattcaag actggtgaat catttacgac tgcttctaca acgaacccat
    88201 gcgcaacaat atgctgtttt agacacggaa atcgtagaaa tggtcaaacg aaaataccca
    88261 gaaagctata agatttctaa aaaaatccgt gtgttgctga ccaaagaata ccagctcgcg
    88321 atcacgaaag aagaactggg ttacttggcc atccatatcg agcgtttaag aagcgcaatc
    88381 gtacaaacaa atgttaacaa tcatgaggag gaaaaaaatt gaaagcatat ctacaacgaa
    88441 tgggacgttc ccttcaactt ccagttgcgg tcttacctgc tgctgcttta ctagtaggga
    88501 tcggaaactg gtgggcatct tatagtaacg acattgtcgc tcatttttta caagctggcg
    88561 gtaacgcagt tttaggtcaa ttacctttgc tgtttgccgt tgggttagca ctagggatgt
    88621 cagtggacaa atcaggtgct gctgccttgg caggtttggt cgcatttgaa gttccagtaa
    88681 atgttttaaa aactgattca gtagctactt tattgaatat caaaccagaa tcagttgatc
    88741 catcttttgc tcagatcagt aacgtattta ttggtattat tgccggattg attgcggcag
    88801 ctctttataa tcgtttccac gaagcaaaat taccgatggc tttatctttc tttagcggca
    88861 agcgtctagt gccaatcttg gctgcctttg tcatgttgct gatcagtgca gtgctactgc
    88921 tcgtatggcc tccggtttac tctgctttag tttcttttgc taaattcatt ttgagtttag
    88981 gctggggtgg tgcaggtctt tatggcttct tcaaccgttt actgatccct acaggattgc
    89041 atcaagcaat gaactccgtg ttctggtttg acgtagcagg aatcaatgat attggtaaat
    89101 ttttggcttc tgaaggaaca aaaggcgtaa caggaatgta tcaagctgga tttttcccaa
    89161 tcatgatgtt cgggcttcca gcaggagcat atgctattta cagaaatgca cgtccagaaa
    89221 acaaagcaaa aacagcttcc ttgatgttag cagcagcttt tgcttcattc tttactggtg
    89281 taacagaacc acttgaattc tcatttatgt tcgttgcttg gccattatat gtcttgcatg
    89341 caattttcac tgggatttct ttagcagtga gtgcattctt ccattggaca gcaggatttg
    89401 cctttagtgc cggatttgtc gattacttcc tatctttgaa aaatccgatt gcgaatcagc
    89461 cattgatgtt gatcgtccaa ggattagtaa cagcagtgat ttactacttt ggctttaact
    89521 ttgctatcaa aaaattccat ctgatgactc ctggccgcga ggaagcagat ctttcagatg
    89581 acgatactgc aacgactaat tcagacaata aatatgcggc acaagcaagc aaaatctacg
    89641 ctgcattagg cggagccgac aatgtaactt cgatcgataa ctgtactaca cgccttcgtt
    89701 tgcaagtcaa agatacaggc ctgatcgatc aaaataaaat caaagccact ggcgtacctg
    89761 gtatgaagat catcgacgga aaaaatgcgc aagtcatcgt aggtacagaa gtacaattcg
    89821 ttgcagatga gatggcaaaa ctacatggtg gtgctgcaac acgacctgca caaacaaaca
    89881 ccgtggttaa aacagaaaca tttgccggtg gaacgatcga acaagatatt tacgctatcg
    89941 caaatgggaa actgattcca atcactgaag tttcagatga tgtattcgcg gaaaaaatga
    90001 tgggtgacgg ctatgctgta ttaccagaaa atggagaaat cttctctcca atcgctggta
    90061 cgatcacaaa cattttccca acgaaacatg ctgtcggaat ccaaacagat gctggtatcg
    90121 aagtcttgct tcatatgggc atcaacactg ttgacttaaa aggcgaacca ttcactttat
    90181 atgtaaaaga aggtcaaaaa gtcgcaagag gtcaattgat tgctttagtt gatcttgccg
    90241 ctattcaatc tgcaggcaaa aacacggata tggtcgttgt attcacaaat ggcgacaaag
    90301 tccaaagctt agaaatcgaa cccgcacgtg atgtaaaagc aaatgataaa atcggctctg
    90361 tttccaacaa agcctgagcc atcgtcttcc ccagactttt ttatcgataa acaaacagga
    90421 gctgagacaa aaatcttgtc tcagctcctt tttccgaata aaaacggcgt tctcctgcca
    90481 actccgcggt aataagtcca gccaaacgta acatatgaag agctattttt tatttgactc
    90541 ttcttattac ttgatgcaga acagcttttt tacaacctct gataaacggt gttcaaaaac
    90601 cagtttatcg gtatgaagtc aaatttatgc aaaaatgtgg aaagccattt tcacaaaatt
    90661 tttcttcata ctcaaaacta atcggctttt tcacaacctc tttatacatt attcctgatc
    90721 caatatttct tttaatgctg cttcaagaga agttaatggt tttcccaata ctttttctaa
    90781 atcatcagag gaaatagcta actgatcctt tttaatatca tgttggatcg ctaaaaacat
    90841 gtcagctgct tcttgaggga atccggcttc ttttagattt tcgacaaacg ctgcatcact
    90901 tgcttcaata atttctaact ttttacctgt cgcacgctct aatgccttgg ctaattcttc
    90961 atagtcagtt agattgccgg ataattctaa gatttctgga gagtcacttt ctactagtac
    91021 ttttgctgca gcttctgcat attcacgttt caatgcccaa cctgtttttc catttcctgc
    91081 agcataaacg aattgaccag tttttaaagc ctgcccaatg ataggtaatt cattctctac
    91141 ataccaatta ttccgtaaaa atgtatggga aatacctgaa tccttaatca ttttttcagt
    91201 gaattggtga tctggcgcta atacactgat cgagtgatcg gcatctgcaa aacttgtata
    91261 agcgatataa gataccccag ctgcttgtgc tgccttcact acattcttat gttcggcttg
    91321 acgatttcct ggaacgccag agacaaaaag caatcgatct attcctttca acgcttcttt
    91381 cattgagtct aaatcatcat aatcaccaat acgtatatta aatccagctt ctttcagtgc
    91441 ttctcctttt tcttcactac gtactagtac atagatttca ttttgtggaa ccatcttttt
    91501 taagaattcc agcgcataac caccaaaacc tccagttgct cctgttacta aataatgcat
    91561 atttgccact ccctattctt tttatttgtt aagatttaca gctgtacatt tataaattac
    91621 acctttatca taaagagtgt ggtaagataa gtcaagcaaa aaaaatgaaa ggatgacaac
    91681 tgatgaagta ttctctgcaa tttagtgatg caatccatgt acttgcctat attgaaatgt
    91741 ttcaagatac ccgatcactt tctagtgaga tgattgccag tagtatcgaa acaaatgcag
    91801 caaatgtacg taaaatcatg agtcagctca aaaaatccgg tttgatcttt acaaaaaccg
    91861 gaaaaccgca gccttccctt gccaaaagtc cagatcagat ttctttacta gatgtctatc
    91921 gcagcatcga aggtaataca cagcttatcc aagtcgatcc aaaaacaaat ccaaattgcg
    91981 tcgttggtgc gaatatccaa gaagctttag aagaaagcta cgctcggctg cagaaaaagg
    92041 cagaagaaga aatggctaag atcacacttg atagtctgat acataaaata gccgttttgg
    92101 aaaaagaaaa aagaccagaa aacacaggcc tcgtagagag atttttataa ttctctctac
    92161 tgtgcctgca tttttctgat cggaatcgtc tacataaatt cttttattct ccgggggaca
    92221 aaagccttgt catgcccctt tttcctctct aatgctcagg tcaaagcgct gaagtcatga
    92281 cgtcttgata aacggtgttc aagtagctga ccctcggcat tagttctgaa aaatgcaaaa
    92341 tatggaaagc tatttcccat ttttcctctc taatgctcag gtcaaagcgc tactttcacg
    92401 acctctatat tatcatggtt tattgactac cggtatccag atttctccca tatatttccc
    92461 gttggtaact cccatttcga ctacagcatt tggaccccca acataggcgt aatcttctac
    92521 ttctcttaaa acatgcccaa aggccgtatt cgtcaaatca tttcttaatt cttcaggcga
    92581 ttctgctaaa gcgattacaa caagatacgt tccttttggg aatgggatga ttcgggtagt
    92641 gtttggaaca tcctgactcg tttgaacacc gacatagtac atcattttcc cgttataggg
    92701 ttcgttgact gtaaagaaat aatgattttc agcagcttgt ttcaatgcat ctattgttcc
    92761 attaccgata actttttgct caaatgcttg tttctctgca gccaaaccga caaaatccgt
    92821 attgctactc ttcaattcca caccaatacc cgtcaccgta aaatctggtt tttcttcgat
    92881 cctatacttt gccatttctc ccaccctttc tattatatta agttcatctt acataaaatt
    92941 aaagggaaat gacacgatta cgctcttttt tctagggata tttttatttt ttaactattt
    93001 ttttgctttc actgcaatcc aaatctcact gtaaacatta ctgaaatcct ctccccattt
    93061 tgtaaaagag atctcgggag cttcgactaa ctcataatct gaagaaggaa gccattctgc
    93121 atagattttc ccccaagtat cttgtaaagt ttggggaaat ggccctttat taggaaaaat
    93181 ggcccaagtg tgttcttcta tagcaagcat ttctaaatca gcgtacggat tttcttttgt
    93241 cgtagcaaag ccaatcatgt gcatcaaact gcccttttct tccaagcggg ctccatcaaa
    93301 tccataagaa acgttgatta cttgatttgg atataaatct cctaacttat gcatctcttc
    93361 cctttgagtc tccgtgatcg tctgcgctaa cttttgtatt tcttgattga ctccttcaaa
    93421 tttgacagga acttttttcg ccacacctgc cacataaaac tgttctttct tttcgatacg
    93481 aaattccatc gattttcctc ctctgacatc aataaaaaat gtcaagcgtg gaaaagattt
    93541 ttgtatgccg tatctgctga tttctgaagg cagatatcct gaccaatcac gaaaagctct
    93601 tgaaaaacct tcgactgttt catatccata tctaaacgcc gtttctgtca ctcttgatcc
    93661 atctaggaga tcgcgattag ccaaagccaa tctacggttt ttgatatagt ccattagcga
    93721 catccctgca ataaacgtaa agatgcgttt taattgatat tcagagattc ctgtattttc
    93781 ggctattatt ttataggaaa gtccttctgt caaatgttct tcgatgtaat cgatcaattg
    93841 gttcagatct ttaagcatat ttatcttcct ccttgcttgc ctttttatca tattatcaaa
    93901 gaaagtcaat tacttgatga atcaggaacc aatttagtca gatgtagaaa atgttgtatg
    93961 gatctaaaga aaattctcaa gcagcgtttc gtaacggata attggtatta tctgcctatc
    94021 tatgttttac ctttagaaaa ataaattttg gggaagacac attctaagta aacaaagcgg
    94081 taaaaatatc ttttattccc ttagttaatc cgcttactac ctactgtaca aagcaaattg
    94141 ttgttcaaac tgctgatcag aacgctgctt ggatttttct ttgcgataca tcatacctct
    94201 tttatcaatt ggaacaatta tttatttcta acaaaatcac ttcatttttg tcagataaat
    94261 aaaaaacaaa aaaagttcca tctttaaaac acgctattct tttacttttt ctcacattct
    94321 ctaagaaaag aaaaggagaa tgaatttttg acaattcgag caaaagtgat tatcaaatat
    94381 gtaaaatcag ctactttttt cttaaacgtt tctttatact aaatataaat aagtgtttca
    94441 tgaaaggaga actagatgaa taattggctt gagtattttc ctgaaaacgt tttggaacgc
    94501 ggttacagct atcatctgca cggttttgtc cgtcatttga actatacaag caagtacttg
    94561 tccgcaacag tttctggcac ggaagattat aaggtagtga tcacttggga tgaaaagacc
    94621 aacatgactt gtgattgtct atatgccata gaaggaaaaa aatgcaaaca catggctgct
    94681 gtcttattcg cttacgaaga acgtccaata aaaaaatcca actactcact ttccgaactc
    94741 tcgtcccttg tttcctccgc tagttcctct cttgtacggg agcttttaac agagattctt
    94801 atagaacatc cgcaatttat cgaacgcttc aaagttaaga tgccatttca tgcaataaat
    94861 tattcagata aattaacaac tatcatccat aaatacgatc atattatcaa gaaaaacaaa
    94921 aacaggaaaa cagcgaagtt catcatggag atgcgaaagt tcatccaaga agcagttgaa
    94981 tcgctcattc agcaaaatgc ctatcttccc gcattcgaat tgatcaatga agtgattgct
    95041 actctcgaaa cattctattg ggagcctgag gatgaacgaa cccttcttct gatagaggac
    95101 tgttattatt tatggaaaga attattagca gaagcacctc atgcagaaaa aaggcaaatg
    95161 ttctcttggt tcgtctgcca agtcgatcat actgacgcct cttattcaaa acgctactcc
    95221 ataaaaatcc tgaaagaaga ctttcgggaa aaagaatttt ctaaccaaaa gaaaaaaata
    95281 gataaaaaaa ctaaagaaac ggtaaagaaa gacgatttca atgaaaaata gcaagtaaac
    95341 gataacaacc atcctaaatc cacaagaaat acagcagacc ttgctttaaa aacacgaatc
    95401 atgcaaagga tgaagtaaag aagacttaac gcgtacccaa tattagtcat ttgactatta
    95461 ggtacgcgtt ttgttttata agtcatgaca aatttccttc gttgctgggt agattttccg
    95521 gcattgttga tagattttat gatacttttc tacattttgt aagagaggtt ccactgcatc
    95581 tgtatattgg ataaaattct gacaacaact ttcaggtgtg tcgaaccact ctaatcttta
    95641 aagataaata ttaaaataac gattaataat attaacagtc tttatttttc accaccaaaa
    95701 acctatcttt gcatatacta aatagacgta tatacacaga taggtttgtc ttgtgaaaaa
    95761 taatgattat ccttaccaaa aaatgtttgt ttttctacat gttcgtactt atcaaaaaat
    95821 atgatcctta ttgaaattac tggctattta tattccaacg acttcttgtg ttaatttttg
    95881 ttatctcata gtaaattatg tttcattttc acttaattta gttgtcacgt gttcgtcaat
    95941 tcgtaaaaac ggtaaatata taaacacact tgcggctata attaatattt gtaaaacagc
    96001 tgctcgccag tctccggctg ttgctaaaaa tcccgatata actggtggcg tagtccaagg
    96061 aatttgcact actacatgat taattaatcc cacagcagtt gcaaaatatg ctactgtcaa
    96121 agaaaaaatc ggtgcaagca caaagggaat aatcagactg atattaaata caataggcaa
    96181 tccaaaaata ataggttcgc ttatattgaa tatagcggga gttaccgata atttagcaaa
    96241 gtccttataa tcttttctac gactgaagat aaatattgca atcaataaag caatcgtatt
    96301 tcccgatcca cccattaccg caaaggtatc tttaaaggcc atattgataa tatgtggaat
    96361 ttgctctttg tttgcataag ctgccatatt ttcttgcata ttctgaatca aaaacggatc
    96421 taacaatgaa cctgatatta ctgcttgatg gataccaaat ccaaaaaata gattagcaat
    96481 agatgtaatt aacaaaaatc ctggtaaacc agttgtaatt ttacttaatg gctctgtaac
    96541 tatcgtggta attagcttat ttatatccat attaaacaat tgaatgatta taaatgacaa
    96601 taaagcaaac gaggtaatcg taataatgat tgggattaat acattaaaag actttaaaac
    96661 agcgggagga atatttccgg atagctgaat ctgtagtttc ttattattag aaagtttaat
    96721 aaataattct gtagccgcta atccaacaaa tatacctaca aacattcctg aagctcctgt
    96781 ataggatata ggtatcacac ccgtcacttc aacagcttta tccgcatttt caggagtaaa
    96841 catcatctgc ataggtgtta atacaacaaa tgtcgaaata gcaactaata ccgctgcaat
    96901 atgatttgta taatttctgc ttgcacacat atgataagat actgctgcta caactaataa
    96961 tgtagttatg cttaatgtac cattggcaat tgaagtacca atttgttgta acgtggtcaa
    97021 tgtactagta tcgacaattt cccccatgaa tcctgttggt tctaagatca ctgaattaat
    97081 aagtgtcaca aatcctgcta acatcataaa tggcataaat gtagcaaacg catctcgcaa
    97141 agttcttaaa tggatctgat tccccaactt agtcgcaact ataccaaatt tttcaaatac
    97201 aatgtccttc ctgctacttt tgcccatatt ttttctcctt tatgcttatt gtaatcgatt
    97261 actttttcca cccaaataat acatcactta tcaaaaaaag taaatacttt taattaaata
    97321 tatatatctt ttttagtata aaaaaataag ctctgaaaaa ataattttcc cgagcttatt
    97381 tttcaacaaa aacagcttca aaaacaaagt cttgatataa atgtctcgat accgaatact
    97441 cgaaaggttt accagacgat agagcaacaa ctttgtctat ctcaatcata aattcacctg
    97501 gatctatttt taatattttt gcgtcaaatt cattcgcagt atctcctttg atacgtatgt
    97561 gcgaagtctt tatttccaga cccagttcat ttgtgatata agcatataca gatttcttta
    97621 aatgttttgg ttccaagcct ggtatgactg ctaatggcat gaatgtttgc tcaatcgagt
    97681 atggttgaca atcaataaaa cggcttcgta caatattata tacatagtct tttacactga
    97741 tacttaattt atcagctact tttttcgatg gaagttcaat tgagaaagta tggatttctg
    97801 aatctacgtg ttcttcacca accacgctaa acaaaccttc gtttggacca agaactcgtg
    97861 agttatcaat tggtgttctg acgacttcag tcccataccc acttctactt cgcaaaactc
    97921 cttcggaaac aagtaagtct acgcctttct ttactgttaa cttgcttgta tgataaataa
    97981 ctgccaattc atctcctgta ggaagtttct caccaatttt atactctcca tttacaattt
    98041 ttttctttat atctaagtaa atttccatat acttcgcaat ttttttcaat ggtgaccctc
    98101 caatcaaaaa catatcttca tctatacttt aacataaata aaccgataaa atccacatca
    98161 aaattataca cacaataaaa aagatatata tatttaaaca aaactattta ctttaagttg
    98221 taagcggtgt tatcattaag tggaaacagg aggtaaaaaa attatgacaa ctatgaaaaa
    98281 gctaactttt ccagatgatt tttggtgggg ttccgcttgg tcagctgagc aagcagaagg
    98341 aaaaggagaa acaggaaaag cagagactat ctgggatcgc tggtttaaag aacaacctta
    98401 ccggttttat caacgaatcg attcctctat tacaaccgat cacatccatc aatataaaga
    98461 agatatagaa ctaatgaaaa aaactgggca taactccttt cgcgtttcta tttcttgggc
    98521 acgaatgttt ccaaatggcg gtaccggaaa agtaaatccc aaagccattt ctttctatcg
    98581 aaaattattt tccgaaatga ataataacgg aatcaaagta tttgccaact tatatcattt
    98641 tgacatgcct gctactcttc aagataaagg aggatgggaa tcacgtgaag tagttgatgc
    98701 ttatgttcat tacgctgaaa cttgttttaa agaatttggt gacttagttt gtcattggtt
    98761 taccttcaat gaaccacttg ggcctatttt aggtacttat cttgaagatt ttcattatcc
    98821 aaacctcatt gactttaaac gcggagcgca agcagcattc ttcactatac ttgcacacgc
    98881 aaaagcaata caagtattta aagaactagc tcttcctagt aaaataggcg ttattctaaa
    98941 tttgagtcct acttttcctc gtagtcaaaa tcctgctgat gtgaaagcag cagaaatcgc
    99001 tgatttattt tatacgcgga gctttttaga tcctatggtt aaagggaatt tttctaaaaa
    99061 gcttatcctg ttattacaag aatataatca gcttcctgaa gattatacga aagaagatct
    99121 tgtatgtatc tacgaaaata ccgctcaaat tcttggacta aactattacg agccacgtcg
    99181 cgtcaaatct cgttcttcta tggtggatac atctagtcca tttttaccag agtggttttt
    99241 cgaaccacat atcatgcctg gacgacgaat gaacgaatac cggggatggg aaatttacga
    99301 aaaaggaatt tatgatttgt gcatggatat caagaaccat tatggaaaca tcgaagcatt
    99361 tatctcggag aacggaatgg gcgttgcgga tgaagaacgt ttcatggatg atacagggca
    99421 aataattgat gattatcgga ttgagtatat caaagatcat cttgcttact tatggaaggc
    99481 aataaatgaa ggatgtaaca ttaaggggta tcatctttgg acatttattg attgctggtc
    99541 atggatcaac gcctataaaa accgatatgg gctggtttca ctcgacctta ctactcaaaa
    99601 aagaacaatt aaaaaaagtg gcgaatttta caagcaatta agtgatacaa atggatttag
    99661 atacgatgta gacttgttag ttgaataata ttctaaaatt ccacttgaag tgaacacatt
    99721 agaaaacaag aataatgaga aataccaata acaaatactt tttcttcata cttcatttat
    99781 acttgtcagt ataagagact gtgacaaatg tagtgatcct cagaagttag gtctggaaat
    99841 ctaacttgag aagatttttt taaactacgt ccagtctttt ttatttgttt aaaattgaag
    99901 ttgacaagtc ttcattaaaa atttagaata ttcacaagtt tgacgacaat tatgaaaatt
    99961 tatgtcgtgc taaaggagtt ttgttattgg atgaaaagtt agatataaaa actgcgatca
   100021 aagacacgtt acctaccgtt tttggttata tcggtattgg acttgcattt ggtatcgttg
   100081 ggaaagctgc cgggtttcat ccgctggtcg tcacgttgat gtccctgctg atctatgctg
   100141 gttctgccca atttatcacg gtcagcatgc ttgctagcca tagtccactg ctctccatcg
   100201 ttctctcgac ctttctagtc aattcccgaa tgattctgat gagtatgacg attgctcctt
   100261 atttcaagaa aaatcggctg cttcaaaatc tgctgattgg cacattgctg acagatgaaa
   100321 gtttcgcact gggaatgaat aaattgaact atactggaca aaagttaaac tttcgttgga
   100381 tgaatacagc taattggatt tcttatttga cgtgggtggg ttcttctctt gttggcgcac
   100441 ttttagggaa ttttattact gatccgaaaa agtttggttt ggatttcgcc atcgtcgcta
   100501 tgtttatcgg gttactttat cttcaagtca tttctgatag aaacacctct aaacgattac
   100561 agttgatcct gattggtctc actttgattc tggtatatgt cggactgatt tttatcccaa
   100621 gtaacttagt gattttagtt gtaacactga ttgggtgcgg attgggggtg tggatcaaac
   100681 atgccttctt ttgagtttac attgctgact attttaggtt gtactttagc aacttggctt
   100741 tctaggatcc taccatttgt cttactgaaa aaattcgatt tgccgcaacc gttgcttgag
   100801 tatttaagtt ttgtcccgat cgtcattatg tctgctttat ggttcagcag tctatttacc
   100861 caaaatatcg gacatctgcc gcagatcaat ctcgaaaatg ctttggcttc agttcctaca
   100921 cttttagctg ctattctttc aaaaagctta ttggtgatcg tcttggtagg aattctttcc
   100981 ctttcgttga ttcgcttatt ataaataaaa aaacaagagc ttatggcaaa ttactgtcat
   101041 gcgctcttgt ttttatcaaa gtcaaataag aaaaaaatcg gcgtattctt gcttatccgt
   101101 tgaacggacc actaacaacg acttttccta atatatgtcc tgtttctacg tctttggttg
   101161 cttgtttcaa gttcttcgca ttcatcccat ctgaatagac cttgctcaat gtactgcgga
   101221 taatcccttc atttgctaaa gcagcaatgt gcgctaaagc ttctccttgc gtttcgattt
   101281 catagttata gtctgtttta gcgaacatat actcccagtc gaagctgata gattggtttt
   101341 tccaaacacc tatatctaac ggctctttga tccctacgat cgttcccaca tgtccaaatg
   101401 gtcggacaag gtgtttgatt tgatcaaagt agcgggtaat atcgaataat acagcgacat
   101461 actctacttg atcgattcct aattcacgaa aacttttact taagtcgtga tgataatcga
   101521 ttggatagtc tacacctgtt tttttcagcc attcaaaatt cttcggactt gcagtcgcgt
   101581 agacttctag gccagcccaa tgcgctagtt gattcaaaat cgaaccgacg cccccagctc
   101641 cgttgatcac taagattttt ttcccgcggt tggcattttc ttctggagtc agaccaaatt
   101701 tttcaaataa caattcataa gcggtcaatg aggtcaacgg caaggctgcg gcttcttcat
   101761 ctgaaagatt ttttggtgca agagcgacaa tacgttcgtc aaccagttgg tattcttgat
   101821 tgcttcctgc tcttgtcgtt gtacctgcgt aaaaaacgcg atcgccgaca gaaaattttt
   101881 gcacttcatc acctacagca acaactttcc cgactccatc aaaccctaag atacgtaaag
   101941 catttcttat cccgttatct tggcgaagtt tcgtatcgac aggattaaca gatactgcgt
   102001 tgacttttac cagaagatcc cgtgaactgg gtacaggtat ctgttctttt tcatcaataa
   102061 agctttttgg atcatcgatc gacaaccctt cataaaaacc gactaccttc attaattcct
   102121 tttctgacat acgaattcct ccttagcttc atcaagttgg agcatccata cagttttcat
   102181 ttcaatatat atagtatgct atttgttcca cttttattca tcataattat tctcttactt
   102241 aaagtaaact atttgcactt ttttggttaa aaaagaggtt gcgacaagac ttttgtcaca
   102301 acctctttta aacggtgttc aatcaactga ccctcggtat tcgctcaaaa aacgacaaaa
   102361 tatgaggagc tatttttgtt tggctgttct tattacttga tgcaggacag ctttttcacg
   102421 acctctaatt cacggtgttc aagcaagaaa tcattgttca ctgaaaagcg ttggatgttc
   102481 tttttcaaag gtgtctaaga tctctcgtac ttcatcagta gatttcgtat tcatcaactg
   102541 aactcgcaag tcatttgctc ctggaaatcc tttgatatag attttaaaga aacgatgtag
   102601 tcctgtgatg gaacgcggga tgatttctga ataatgatct tgcagatcta gttgcagacg
   102661 aagtagtccg atcagttctt tttctgtatg ttccctcggt tctttttcaa atgcataagg
   102721 attcttgaaa atcccccggc caatcatgat gccatctacg ccatattttt cggccaattc
   102781 ttctcctgtc tggcgatctg ggatatctcc gttgatcgtg atcagtgttt gcggagcgat
   102841 ttggtcacgt aaagcaatga ttttagggat gacttcccaa tgtgcatcta ctttactcat
   102901 ctctttacgc gtacgcaaat ggacagaaag attggctata tcttgatgaa gtaggtgact
   102961 gatccattct tccatctcag acatttccgt atagccgata cgcgtcttga cgctgacagg
   103021 caaaccgcca gctttagccg cttcaatcaa ttgtgccgct acttctgggc gaaggatcaa
   103081 gccactgcct tttccacgtt ctgctacatt agggactggg cagcccatgt tgatatcgat
   103141 tccttggaag cccatttctg ccatagcaat gctcatttct ctaaaaaatt ctggcttgtc
   103201 tccccaaata tgggcaacca tcggctgttc atcttctgta aaggtcaatc ttcctcgtac
   103261 actgtctttt ccatctggat gacagaaact gtctgagttt gtaaactcag taaagaagac
   103321 atccggtgct gctgcatgct ttacgacatg gcgaaataca acatctgtta catcttccat
   103381 tggtgctaaa acaaaaaaag gctttggcag ctctttccaa aaattagtag tcatttattt
   103441 gttactctcc attctatcaa gcagtgaagt cgcaatcttt ctcaaaaaca ttgtcattat
   103501 actaggaaac aaaaaaagaa gcaaagtgaa cagcttaaaa gatgtttctg attgagcaaa
   103561 aaaagactgt gacaaaaatc ttatcacaat tccttttccg gaataaatgg tgttcaaaag
   103621 cttgcatctg cggtaataag tccaaccaaa cgaaaaatag gaggagctat ttttgtttgg
   103681 ctgttcttat tacttgatgc agaacagctt tttcacaacc tctccactaa acggtgttca
   103741 atcagctgac cctcggtatt agctcaacaa acgacaaaat atgaggaact attttcccgt
   103801 ttgtttctct aatactcagg tcaaaacgct gatttcacaa cctcttttct aacttctcaa
   103861 tcctcttcct ttattgatca aataccaagt aacggcgtac aaaacaacga tgaatagcac
   103921 aagtacgatc atcgatgttg ctactggcac atcgattgtt ccaaggaatc cgtaacggaa
   103981 accgaagatc atataaacga tcggattgac tttcgaaaca gcttgccaaa atggcggcaa
   104041 catagagatg gcatagaaaa cgccacctag ataagttaac ggctgcaaga caaatgtagg
   104101 cacgatcgat acatcgtcat aagactgagc aaaaatcccg ttgagcagtc ctgctaacga
   104161 aaacaagatt gccgtcatca ataatgtaat gatgaccatg aaccatgaat acacatgaag
   104221 tggtacaaag aacaaggaaa tgatcgttac gagagagccg acgagaatac tgcgacccac
   104281 tccgccaatc acaaagcccc aaatgatcac atgcgtagga acaggcgcta ccaagatttc
   104341 ttcgatgttt ttctggaatt tttgagagaa gaaggacgaa gaaacgttgg catacgaact
   104401 agtgatagca gacatcataa tcaaaccagg cacgatgaat tccatatatg aaaaaccacc
   104461 catgtcaccg atccgaccac cgatcatttt tccgaaaata acaaagtaca aggatgtcgt
   104521 gatcacaggc ggaaccagtg tttggaccca gatacgcaaa tagcggttcg tttccttgat
   104581 agctaaactt tttaatgcag taaaataaag attaaacatt tttctcctcc gtatgattat
   104641 tttcttctgt gattttcaaa aacagttctt ctaaacgatt cgacttattc ctcatagaaa
   104701 gaactttgat tccttgtctt gtcagctgat cgaagattcc gttcacccct tgatttcgtt
   104761 caacttcgac agcgattgtc tgttcatctt caaatgtaca agcgtatcct tcaatcacag
   104821 gtttggtctc atatggcgct aaatcaaaga taaatgtttc aaattgcagt ttggatagaa
   104881 gctctttcat actagtattc tcgatcagtt cacctgattg gatgatcccg atgttgcgac
   104941 aaagcatttc agcttcttct aaataatgcg tcgtcaaaat gatcgttgta ccattttcgt
   105001 tcaattctcg gagaaactcc cacatctcgc gtctcaattc aatatcgact cctgctgttg
   105061 gttcatccaa gatcagcaaa cgtggttcat gcatcaatgc cctagcaatc atcagtcttc
   105121 gcttcattcc cccggaaagc atacgtgcac ggacatttct cttttcccaa agattggact
   105181 gcttcaaata tttttcgctg cgtttcatcg cttctttgcg agagacaccg tagtagcctg
   105241 cttgattcac gacgatttgc tggaccgttt caaagggatt gaaattgaac tcttgcggga
   105301 ctaatccaat ctgttgcttt gctcggacta gatccgtatc cagatcataa ccgaagacat
   105361 tcacttttcc agatgtttta ttgactaaag aagtaatgat tccgatcgtt gtagacttcc
   105421 cagctccatt tggtcctaat agcgcataga aatctccttc tttcacggaa agatcgattc
   105481 ctcgcaatgc ttcgacccca cttccataaa cctttttcaa attatttatt tcaagcgcat
   105541 ttgccattta tttcctatct ccttatagac atgattcttt attgtatcat ggaatacagc
   105601 agatgaaaaa acaacttggt tccagcgaaa aatcaggttg ctgggctcag gaaaatctcc
   105661 taatgaaaga catcaatcgg acgaagtcag acaacctttt cagaaaaaac aaatcgaaga
   105721 taaaggatta ctattcatat gtgcaacaaa aggagcgtga gacaaaagta aagaacactt
   105781 ttgtctcacg ctctaaacac gtataaacgg cggaagcaaa agccaagatt tttgatccaa
   105841 taaatacttt cgatcacttc gcctatccca atacatgcca ctgccgcttt tcatcaaata
   105901 acgcggcttc cgtttttgcg gtttctacta aagtctctgg gtaatccaaa actttcagca
   105961 aggcaatcgc atttcgggtc cttgaaggtc cttcttttaa tttgaaatca aaactgattc
   106021 cttttccttc tgttacttgt tcttcaaagt ggacattctc acaataattc tttaaaatct
   106081 ctgtcaattc gatatcatgt gtggcgacaa atgctaaaga tggatactcc gcaagccacc
   106141 gaacgactga agcggaagaa gcaatccgtt cgatcgtatt tgtccctttt aaaatctcat
   106201 ctacgaagca gtagcagcgt tctttggaag ccacttggtc caacaaacgt ttgatcgatt
   106261 tgatttctga aacaaaataa ctgtcgcctt caaataaatc atcttcgatt gccatggaag
   106321 tcaacacatg tccaggttgc agggtaaact gttctgcaat cgctgtttgg atcgtctgtg
   106381 ctagaataca gctgatggct atacttttga catacgtaga ttttcctgat gcattggaac
   106441 ccgtgactaa tgtattcttc tgccagttga ccggattcac taccgcttct tttaacaatg
   106501 gatgatagat atctttcgct gtcacaccgc cctcagtaaa ctctggtcgg caagtgatgg
   106561 gcatataagt acggtaattc aatattgcag cggctacttc taattctccc agcaattccc
   106621 acagcttgat ggcttcagtt gaatgctttt ctaatttagt gagaacgaaa tgataggaga
   106681 taaatggcag catgaacatg atgttgacat attcaaaaat caactctgct tcactcccac
   106741 tcttcatacg aaatgaaaaa cctaatttag ggatgtctct taatggttta gtttgctgtt
   106801 tcagctcttc ttgaagtggt gtcggcagtt ttgctagctg attggcggct gaaatcgttg
   106861 aaaccaaata actcatgcta ttcagctctg tttctagtgt ttgtttcttc actagataat
   106921 agaccgtatt gaatactaac ccaccgattg ccaaatagat tcctagtact tgtcctaaaa
   106981 gaagcaaacc caaacccacc agcggaagca agccaagaaa aataaataaa cctacgcgtc
   107041 caatcttttt actttttcca tccgctagat attgttttgc aaaattatga tctttttttc
   107101 ctaaacgagc gaattgatat tggatttttt ctctcagttg aggattttcc tgataaaaag
   107161 caatcagttt ttctaactgc tgatcttcac caaaatcaaa gctgcgtaaa cgttgataca
   107221 acgcttctga acctacacta gaataggtac tgttgattcc ttcgaatact tcgaacatat
   107281 ccaggtcata ccaagtaaga tcatcgattt cgctatccca tgaacggaac ttcttctccg
   107341 tgagccatgc ttccttcaag ctttcttctt tatcaaatct cggttgataa ggaatttttt
   107401 cccatttgct acgaactgtt tcctttaatt tcaaacgtga aaaaatgtct aaagcaacta
   107461 ataaaataac agctgcaata ataattaaaa cgataaattc tctttgaccc atatccctct
   107521 ccgttccttt atttgttagc tttagtatag cactgttgcg ctatttgaaa agagggtaaa
   107581 aaaacacctt tcaaaataaa aggtgttttt ttcttgtata ttacatattt atctctcatt
   107641 tatcaaaatt gacaaataat catagggagg aattgttgtt aattcttgtt tattctccag
   107701 tgcattgaca actgtcccaa gggaaaggat agcctcaggt actatttttt cttgttctgt
   107761 tggattagct aagcgaagaa caaatccttg ttcatgaaaa taagaaggat aaatcgatga
   107821 tacaattaag tcttcaggta attcaatcaa tgaaaattta cgaggaacat caagttcatt
   107881 ttgtaatggc caaatcttgt tatctaacct gtgaataaat acattcagtt tctgcttttg
   107941 ataagaaata gattgttcta accgtttttc tgcctgtttt gccactttga actcattaaa
   108001 tcttccttct tctacattaa atccaaaagt gactttatac tcacctatct cttgagctag
   108061 cggtgtttcc atcatgacat gcccttcatt tgtcgtatct cctgaagctc gcccaggtcg
   108121 ccaagcaaga ttaggttttc caagttcccc tgttgttgaa aataaggtta tgattaattg
   108181 attttctata cgttcatact cttttaaacc atctgcataa aacgtaaaat aatgatcatt
   108241 ttctgccaca gagacactct tatcgaatat ttctaaatta acaggtttct ctaccatagt
   108301 ttctgaccaa ttcggaggaa tgacatcagg ggtattttca ataaatccgt tttgaatttg
   108361 tgcaattgat ttttcagatt tatctaaaac attaattgct aagcgcatac gatgcgagta
   108421 aacagtatta tgaattttga gtgtgccttc aacgatttga ctttctccca gactgaacga
   108481 tatttcataa gatatttctt ccatttcggg tttttcagat aaacgatctt ttaaatcttt
   108541 aggaagttga gcttttccgt aaacaactaa tgtttcttgt aaagaatctt tatatacttt
   108601 agctgtttct aattttagag atagttctgt atctccttct aatggtgaat aatcatacgt
   108661 atcgccatca tttgctgaat cgatcaaatg aacgaaatca agatattgtc tatctccaac
   108721 tatcaaattc aattgcccat ctttaaaaga aagtttatat ttattgttac ttatctcagt
   108781 tttgtctatc tcgattgctg tttctaattt ttcattattt ttctcaaatt caacaacagt
   108841 gtatcctagt gctggcacat ttacatttat ttttatgtca agttcataat aagctggttc
   108901 atcaaaatat tcgaaacctg tagcaatcat ccgataagca tttttccgtt ctggaaaata
   108961 aacttctttt tcaatgatat tatagggact acctttgaag tgtattttct ttgaatcagc
   109021 tactacatgc acgattttcg atccactata attctcagtc gttgtgttga aaacaaggac
   109081 ttcattcgat tttagcttta atgcatcagc catccgtttt acaataaggt tttcgattcc
   109141 gtcagctatc tcattagctt ctttgattcg atggaaaata tcttccgcaa cattatctga
   109201 aacacagcca ccaatactat catgagcctg gttttgcaaa atctttttcc ataaccgacg
   109261 aagtaggcct ttactgatat ggataccatt tctttctgca atcagcatga gcggctcaac
   109321 tcgttttaga attttttgtt ccaatttaaa attttctatt ttcatttttg ttcttactga
   109381 gccaatagaa cgatgaacac gagagtaaac cggctgacga agttcacctt tatattcagg
   109441 taattctttt ttgtttctga taacttctac ataagaagga taatctgaaa taatattgtg
   109501 gaatttactg tatttattca acttctctac gacttgatca aaatttaaaa tcattgactt
   109561 ttgatctaca cccgaaggta ctaaaatatc tttgtttttt ccttgcatat gaagcatttc
   109621 aactgcttca tccaactttt cttttgcaaa ttcaggaata tctttaattg cgtctaaggt
   109681 catcattcca gtcatataac caaatggaaa attcatggca taaacaaatt tatctcccaa
   109741 tcctttccag ataaaataag gagatggaac catcgtttca aagtttaatc ctcgccaaca
   109801 catgaaatta tcgatttcta cttgatgcaa aagtgtaggc aaattcgcat tgaacccaaa
   109861 cgtgtcaggc aaatacccta ccatcattgg atctccatat ttaaggatcg tatcactgat
   109921 tccaatcatt aagtttctta aaattgattc agcatccact aataaggcat ctgtttgtgc
   109981 ataccacggt cccacgaata gttgtcctgt tttcatcagt cgttggataa ccttaagctt
   110041 ttgcggattg atctccaaat actcatcgac gattgaagtt tgtccatcta aacaaaagtt
   110101 tactttaggg ttcctttcca attcatctag aacttcagta aagacctgat cagacaatac
   110161 gatggcatct tcatttgtaa aataccattc tctgtcccag tgcgtgtgtt gaattatatg
   110221 tgcatttacc atatcagatt ctcctacttt ccttcttcta ctgaatttac ttgttgttct
   110281 tttttatact tgatggattt tagtgcattg accattaacg ctgtaaccac tcccccaata
   110341 acaattgata ctaaatataa aggtatattc gtaacaaaag gaattgccat aaaggttgaa
   110401 agcattgttt cttgagtaac accaaaaaat ccatttagac cacatgctac tgcagtgccc
   110461 accacgatgc ttggtattac tcgtaaagga tctttcacgg caaacgggat cgtcccttca
   110521 gtaatcccca caaggcccat aatcatagct gtctttgcat tatctcgttc cgattcatca
   110581 taaagttttt tgtttaaaat tgttgctaaa aacattccaa atggcggaat agcaattcct
   110641 aataacatgg ctgtactagg tccaaagctt ccgcttgaaa aagaagcaac cccaaaagca
   110701 tatgcgattt tattgatagg tcctcccata tcaaacgcca tcattcctcc taaaatagcc
   110761 gacatcaaag cagcatttgc tcctgacatg ccttctagcc aattagtcag tcctgttgtc
   110821 attgcttgaa taggcgtagc tagtacatac cataatatga ttccaacaac taatgtagta
   110881 acaaaaggaa tcagcataat tgattttaga ctaataactt gcatcggtac ttttactttt
   110941 ttcaatgcat tagctaaata gccacaagct aatccagcaa ccattcctcc aataaatccc
   111001 ataccaagat tttgacacat catcccagtg ataaaagctg gtgctagacc gggacggtca
   111061 gcaattgaat atgcgatata ggctgaaatc atcggtacga taaactgtaa tcccgtctga
   111121 ccaatcattc gcaggactcc ccagaatgta tccgtctggt cccaaacacc tgtgccacca
   111181 aaaatgactg caatagaagt tagtaaccct gctgctacga caagcgggat catataggaa
   111241 accccacttt ggaaatgatt tagaatttct cttccttgtt tcttgaattc cattagtaac
   111301 tcccccacga tatatttttt atttgttaat taatgcaatt gcttcatcaa tcgtctcttc
   111361 accatctttt actacctctg aggtccctac tgttaaaatc ggtttaccgt caaagcgacc
   111421 tcgttctgaa acatttgtat ctactgcaaa tatgacaaca tctgcctcgg aaacatccga
   111481 aattgaaagt ttgttttcta tccccatagc cccctgtgtt tctactttaa tatctaaccc
   111541 tttcttcttc gcaaactttt ctaaattagc ttttgccata taagtatggg caatccctgc
   111601 gatacatgct gttactgcta ctatttttgt catatgaaat tcctcctttt aaaatgatag
   111661 agcttttacc aattcttttt tatttctcga atcttttatt cttttaacaa actcgtcatc
   111721 catcaatctt ccagataatt ctgctaaaat acgtaaatga ttagtacctt tatcagtgtc
   111781 ttttattgct aacaaaaaga cgagatcaac tggttgatca tctaatgcgc cccattcaac
   111841 agaccgcttt aacttggcta cagccactgt tgattcaatt actgctaggc ttttgccatg
   111901 aggaatggcg atatttttcc caattcctgt ggtcatatgg ttttctctgt caaatacgtc
   111961 tttttgaaat tgtttgtggt cagacacaaa cccattagtc tctaacaatt tcgataattt
   112021 ttcgactaca ctttttttat cagatgcatt taaatctatc actatattat ttatattgat
   112081 tttttcactg atcatttata agccttcttt caatatataa actaattttt caaacgacga
   112141 actcgttttt aaatttgcta ataactcatg attcgaagcg atttcattga atgtttcata
   112201 aattttattc aattcaggtt ttactttttc attaagtgca aaaaagaaaa cagaatgaac
   112261 aagtgtccca tcccaatcaa ttccttctgg attaacgtag acacagatta ctggcttttt
   112321 tataaaatct acttcgatat gcggcatagc aaaatcattc atcgctgttg aagatagttt
   112381 ttctcttttg attgctccct ctagtatccc ttctttcgcg taattttctt ttaccaattt
   112441 tttggtaatt atatttaaaa cgttcacttg attatccttt atttggtggg taaaaaagca
   112501 aaatcgttta tctaataaat ttagaaaagc atcttctgtt tgttcattta actgtagata
   112561 ctgttttact tttctgacat ctgcccctaa catcaaagga ctaacaacaa taacaggaaa
   112621 atttgtattt tcaatcggaa tcgtgcttat aacaagttca tcagtgactt ttctatcttg
   112681 tagttcattg atgcttagta catcttctat ctcaaccata tctgcaaaac cgttatttat
   112741 tctttgctct aataattttg atgttccata tccgctacta caaactaata tcactttcgt
   112801 tttgtcaggt ttgtaacgat ccaaaagtga ttgaatatgg agtgttacgt aagcgatttc
   112861 atcttcatta aaatgtatac agaatctctc ttcgatttcc tctaataaat ctacacttat
   112921 cataaatgac cttttaaaag aatatttgat tttgtccgta taagggttat agatatttac
   112981 tcccaatttg agtcttttaa ctgctgcatt gagatgtaat actagattat tgatcaattc
   113041 ttcatcagga taaaattcat ttaatttaat tcttactaac tcttgtagct caacacccat
   113101 atctaaatag gaaagcttcg tttgatctaa gtcgtgattc atagcatttt tttcaatcga
   113161 agtaatgtga atgtcaacat aagcttgttc atctattggc acaataattg aaaatgcttt
   113221 ttcaatcatt ttgattaaaa taagtgtatt ctttgagatt tcttcttcag aattattact
   113281 acgtgataag aaattgcttg tttcaatcct ttctatcgca atagcaaggt ggatagctaa
   113341 agtttgaaat tcataatctg taaatttcaa ttgtgtctta ttcatgtatc gattaagcaa
   113401 ttcggctact tttttcaata tgtccttatt taagaaatta ttcgcttcac caatgaaatt
   113461 gatattcaag tctaagagat ctgttttact gttagatgct gtaaagccac cccaataata
   113521 gttcacaact tctgacatca accttctttt acttttttca tcagctttga tgcgcacgcc
   113581 atttcgatct gaaactaagg acatcccatg cttgtttagt ctttttctaa catctttcat
   113641 tgtgctttct accgtcgacc tgctcacgta taattcgtca gcaagatttt gaatctttag
   113701 agaatcatta gtattcaata atttagcaaa cacataagtt agacggtcct ccggagagtc
   113761 tgcgatattg attttatgac tggataataa atttcgaagt gtttcttttt ttccaataag
   113821 cgaaacgcct tttttaggtt gaaccaacat ttgtacatct ggcatttttt ggattatttc
   113881 tttcaaaatt aaaatatctt tgctaattgt tttcgtagat acatataatt gttctgataa
   113941 ttctttgtaa gttgtgtgct cattttcaat caaaaagtat agtattttca tttgccgttc
   114001 gctaaattcg atgcccatta tcttcacctc ctgaatacag cttaagtgta caattattat
   114061 ctctattttc atacagaaga aagttcttga ttaatgtaga agtttctaat attcttgatt
   114121 atttcaaatc aataaaaaag cctgcaatat ttatatcaca ggcttttttc tctattaaat
   114181 atatataaga taatacattt ttctactagt tctcactaat cttgtataat taaaccctcc
   114241 gcaacttgac ggagatggag aagatgcccc cgtctaatct agcggtcatc tctcctccca
   114301 ttccttccac caattttttt gacaggtaaa gcccgattcc gctagattcc gtatttgtgc
   114361 ggctggtatt ttcggtataa aaacgctgga agactttttc aacagaggtt tgcgttcctg
   114421 gtttgatagg attggccact gttaatcgaa tgaatggatc ctcttttgaa aatgtcacct
   114481 ttgcttgtcc atctgcataa cgcaataaat tctgtatgat attttgcagc actcgcagca
   114541 acatgagcgg atctgctaat acagttgtat ttatcgagtg ttcagcgaac gctacctcga
   114601 tatttttctc agaaaattgt tcataaaaag agaaaaatac ttcttccacc tctttagaaa
   114661 aagaaagcgt atggagatcc atttcttttt gcttcgtctc tattcgagca agatcataaa
   114721 actgatccgt catactgagt aatcttgcaa cagattgttt gatgatcgtt aaatacttct
   114781 cccgtttctc cgtatcctct acctgttcca gtaattgtac ataaccgtta atagcagtca
   114841 aaggagttct gagatcatga gaaaggccga cgatcgatag tctgatgttt tcttccattt
   114901 cttgaaatgc ttgtcctttt tgttcatttt catccaacca tgtattcagt gcatctacta
   114961 ggtccattag attcttttct cgaaattcga ctgaaagacg accaccatat tcttgtcgtt
   115021 cagaaagagt ggcgattttc ttagttaatc tcttgatttc cctttgcagg aagaaaataa
   115081 aacttcccat aaagataaca acgagaagca acagccattt aatcaaatct aaaccccact
   115141 ccccacaccg tatgaatata cacagcatgt gcctttgcta gcttccctcg aagattggaa
   115201 atatgcatat tcactgtttt ctcactatca aaatatggtt cttgccaaac tgtttcataa
   115261 agatttgctc gcgaaaatac ttttttcgga tgttctaaaa aaagcttcag cagatcgaat
   115321 tctcttcctg tcaaatggac agccacacca ttcacattta catccctcga ctctttgtcc
   115381 agtaagattt ccttgtaata gatgcgttgc gacagatctt ttacttggac agaagtctga
   115441 tgacgaagtt ggatgccgat tcgtaacaaa agttcttcga tatcaaatgg tttcgtcaca
   115501 tagtcatccg ctcctgctgc caacaaggaa actttatctc tttgatcgct ttttgctgaa
   115561 agaacgatga tcggaacatt cgatgtctgt cggatttctg ctaacaatgc ttcacctgaa
   115621 agccctggaa gcatgagatc tagaaggatc aaatcgacag gatatgtgct taacaataat
   115681 tttccttccg tacctgaata agcatcaaga agtttgtatc tctgttcaag aacctcctcc
   115741 agtaaggaat gaacagcttc atcgtcttcg ataatcaaaa tagtttccag caggaccacc
   115801 tacttaatgt cagatttttg gaaaataaac attcctagag ctgatgatac cacgattgtt
   115861 actagactga ccagaatcat tttcggtgct tgcgaataag ttgcttgttg cgcaaagtcc
   115921 gtatacatga gccacgtttc cgtaaaccac tccgttagaa attccaagcc tttgattttt
   115981 gcgaaaacac cgggaatgaa ggccgttccc atgatataaa gaaaagaaag aagcaaactg
   116041 acactggtac ttctagtgat gaatgtcaaa gccactggaa cagataatgt ccctaatgtc
   116101 agcagaagca atacaggaaa tacttttaga acataatttc catagttttt gattccctca
   116161 acttcaccaa atccgaaagc aagtgtgtaa gcaagcatgg ctagtcccgt gatcaaaacc
   116221 acgccaagtg ttgtgatcaa aagcgtaacg agaagtttcg acacataata agtcattcga
   116281 ttccgtccta aagataacgt attgcgaatc gtaccgtttt gaaattcttc cccaatataa
   116341 aagcacgcaa atgctgcgaa agccagaaga atccatgcag ccattttttc taccattaaa
   116401 atccctgttg caccgttgat gactgtatcc gttgcttggt ttaattcatt gccgtatttt
   116461 gtatgcatat accattcgtt ccaagaataa aaaagcggaa gtacgaccat cagtcctagt
   116521 gagacccaca ttttacggct cttccatact tttatttttt cagcagttaa tagattaaac
   116581 atgtggattg cctccagtta attgtaagaa ataatcttcc agtttttgcc caactaggtg
   116641 aataccatcc acaggaactt ttgcttctac caatgcttga ttgatggctg ctacttggtc
   116701 aattccttca aaaagctgga taacacggct agattgtaca aaataatccc tataaccaag
   116761 agagtcgaga accgttactg cttgttccgt cgccgaagtg tcaattttta taaattgacg
   116821 agattcttgt gccaattctt ctttcgataa ttctttgatc aagtgtcctt catgcaaaat
   116881 accatagtga gtggcgatct gtgacaattc atctagcaag tggctagaaa gcaagacggt
   116941 gatgccccgt tcttttacca aacgttggat gatttctcgc atttctgcca tccctgctgg
   117001 atccatccca ttcgtcggtt catccaaaat caaaaattct ggattcgtga tcagtgcatt
   117061 tgcaatagct aaacgctggc gcatacctaa agagaaattc tttgctttct ttttccctgt
   117121 attttccaat cgcatcattt tcagcaggtc ttcgatttct ctatccgaca caccgccgtt
   117181 cgctgcttgg atacgtaggt tatctcttgc agttaattct ggataaagag caggggtttc
   117241 aatcgattga cccatacgtc gtcttgctgc ctgcaaatcc gtcgttccaa ataattcgat
   117301 cgaaccttca tcaatcgaga tcaaacccat aatagttctc ataaaggtag acttacccgc
   117361 tccattttcc ccaatcaatc catagatcat tccacgtttg atttcgattg atactttatc
   117421 taatgctttg gcagcaccgt attttttggt tataccagtt gctttcaaga ccgtttcatt
   117481 tccaaaatcc atacttatcc ttctttctta ttgatgagtg aagtatagtt gacgggtata
   117541 aagaaagcag ccagaaatgt ataaagaaag tgtaaatttt ccaattgaat gtcaaaaaaa
   117601 acaatcggac aatacgtgtc tttcctatta tcaggcagtt tgttatacgc ttgtatcagt
   117661 ttcctgattc tttcttttcg accaaagtcg tttctctgta ttcctcttca ctgaaaattt
   117721 ttgcttcttt cagatcaagc ttgagtaatt ttaccagacc ggttataagg ataactatag
   117781 caagactccc tcccagaaaa attagactgt ctcgcagacg tggttgcgtt gtcttccaat
   117841 caaagagcgc accaaacagt gctaatccaa gtggactggc caccatggta atcgttgtta
   117901 tcgttgacat aagtcgtcct tgtatatgcg ttggaacttc tcgctgaagc cagaccatat
   117961 aaaaagcaaa agaaaacatt aatccttaaa aacggactta tgaaaaataa tacatgccgt
   118021 ttgggattta caattagcta ccaaataaga ggttatgaca aaagactttt cacaatccct
   118081 tcttccgaat aaacggtgtt ctcctgccaa ctcctcggtc acaagtcaca atatttatca
   118141 aacatgagaa gcaatttttg taaattcctt cttatttctt gaagctgacc acttctgtca
   118201 caacctcaga agcggataaa taatattgtt tgatgatact ttatcttttt cccgtttgtt
   118261 tatagatcga atgatattat agaccaagaa tcctacctat taacatagct aaaaacacaa
   118321 aaaaggcact ttccaaattg tggaaagtgc cagaaaagtt aggattaagg taactgcgct
   118381 cagcattgct tcgcccagta cgattctcgt aactcgtgaa ctcgaacgat aatcgcaatt
   118441 aacgttttga gaactgtgaa gctttacggg ctttcttaag acctggtttt ttcttcgtat
   118501 cattgaataa gtttgaaaaa ccttgattta taaaggtttt cccgtaaata tattttatta
   118561 tatttgatta aatttgaaat g
//
LOCUS       DACDIG010000003        88314 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.003, whole genome shotgun sequence.
ACCESSION   DACDIG010000003 DACDIG010000000
VERSION     DACDIG010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 88314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 88314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..88314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.003"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            9..587
                     /locus_tag="IV247_000238"
     CDS             9..587
                     /locus_tag="IV247_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8776338.1"
                     /translation="MNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNT
                     MGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGI
                     YLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKE
                     LMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
     gene            complement(827..910)
                     /locus_tag="IV247_000239"
     tRNA            complement(827..910)
                     /locus_tag="IV247_000239"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(874..876),aa:Leu,seq:gag)
     gene            1293..2510
                     /locus_tag="IV247_000240"
     CDS             1293..2510
                     /locus_tag="IV247_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP8776339.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            2694..3530
                     /locus_tag="IV247_000241"
     CDS             2694..3530
                     /locus_tag="IV247_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8776340.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            3606..4754
                     /gene="nagA"
                     /locus_tag="IV247_000242"
     CDS             3606..4754
                     /gene="nagA"
                     /locus_tag="IV247_000242"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAP8776341.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            4975..6033
                     /locus_tag="IV247_000243"
     CDS             4975..6033
                     /locus_tag="IV247_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAP8776342.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            6052..7071
                     /locus_tag="IV247_000244"
     CDS             6052..7071
                     /locus_tag="IV247_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAP8776343.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(7173..7889)
                     /locus_tag="IV247_000245"
     CDS             complement(7173..7889)
                     /locus_tag="IV247_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP8776344.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(7858..8643)
                     /locus_tag="IV247_000246"
     CDS             complement(7858..8643)
                     /locus_tag="IV247_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP8776345.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(8634..9383)
                     /locus_tag="IV247_000247"
     CDS             complement(8634..9383)
                     /locus_tag="IV247_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAP8776346.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            9681..10205
                     /locus_tag="IV247_000248"
     CDS             9681..10205
                     /locus_tag="IV247_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8776347.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            10221..11720
                     /locus_tag="IV247_000249"
     CDS             10221..11720
                     /locus_tag="IV247_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAP8776348.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(11808..12410)
                     /locus_tag="IV247_000250"
     CDS             complement(11808..12410)
                     /locus_tag="IV247_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8776349.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            12514..13470
                     /locus_tag="IV247_000251"
     CDS             12514..13470
                     /locus_tag="IV247_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAP8776350.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(13775..14731)
                     /locus_tag="IV247_000252"
     CDS             complement(13775..14731)
                     /locus_tag="IV247_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8776351.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            14905..15465
                     /locus_tag="IV247_000253"
     CDS             14905..15465
                     /locus_tag="IV247_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8776352.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            15458..16283
                     /locus_tag="IV247_000254"
                     /pseudo
     CDS             15458..16283
                     /locus_tag="IV247_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
     gene            16293..16910
                     /locus_tag="IV247_000255"
     CDS             16293..16910
                     /locus_tag="IV247_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAP8776353.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            16928..17611
                     /locus_tag="IV247_000256"
     CDS             16928..17611
                     /locus_tag="IV247_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP8776354.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            18294..19577
                     /locus_tag="IV247_000257"
     CDS             18294..19577
                     /locus_tag="IV247_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8776355.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            19593..20480
                     /locus_tag="IV247_000258"
     CDS             19593..20480
                     /locus_tag="IV247_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8776356.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            20497..21333
                     /locus_tag="IV247_000259"
     CDS             20497..21333
                     /locus_tag="IV247_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8776357.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            21336..21959
                     /locus_tag="IV247_000260"
     CDS             21336..21959
                     /locus_tag="IV247_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8776358.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            21956..23248
                     /locus_tag="IV247_000261"
     CDS             21956..23248
                     /locus_tag="IV247_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8776359.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      23607..23704
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            23820..24401
                     /locus_tag="IV247_000262"
     CDS             23820..24401
                     /locus_tag="IV247_000262"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAP8776360.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            24404..25711
                     /locus_tag="IV247_000263"
     CDS             24404..25711
                     /locus_tag="IV247_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAP8776361.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            26127..26615
                     /gene="purE"
                     /locus_tag="IV247_000264"
     CDS             26127..26615
                     /gene="purE"
                     /locus_tag="IV247_000264"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAP8776362.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            26599..27732
                     /gene="purK"
                     /locus_tag="IV247_000265"
     CDS             26599..27732
                     /gene="purK"
                     /locus_tag="IV247_000265"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAP8776363.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            27745..29040
                     /gene="purB"
                     /locus_tag="IV247_000266"
     CDS             27745..29040
                     /gene="purB"
                     /locus_tag="IV247_000266"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAP8776364.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(29220..29597)
                     /locus_tag="IV247_000267"
     CDS             complement(29220..29597)
                     /locus_tag="IV247_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8776365.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            29853..30581
                     /locus_tag="IV247_000268"
     CDS             29853..30581
                     /locus_tag="IV247_000268"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAP8776366.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            30581..30835
                     /gene="purS"
                     /locus_tag="IV247_000269"
     CDS             30581..30835
                     /gene="purS"
                     /locus_tag="IV247_000269"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAP8776367.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            30837..31508
                     /gene="purQ"
                     /locus_tag="IV247_000270"
     CDS             30837..31508
                     /gene="purQ"
                     /locus_tag="IV247_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAP8776368.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            31509..33731
                     /gene="purL"
                     /locus_tag="IV247_000271"
     CDS             31509..33731
                     /gene="purL"
                     /locus_tag="IV247_000271"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAP8776369.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            33716..35155
                     /locus_tag="IV247_000272"
     CDS             33716..35155
                     /locus_tag="IV247_000272"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAP8776370.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            35187..36230
                     /locus_tag="IV247_000273"
     CDS             35187..36230
                     /locus_tag="IV247_000273"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAP8776371.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            36227..36805
                     /gene="purN"
                     /locus_tag="IV247_000274"
     CDS             36227..36805
                     /gene="purN"
                     /locus_tag="IV247_000274"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAP8776372.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            36802..38337
                     /gene="purH"
                     /locus_tag="IV247_000275"
     CDS             36802..38337
                     /gene="purH"
                     /locus_tag="IV247_000275"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAP8776373.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALVFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            38353..39627
                     /gene="purD"
                     /locus_tag="IV247_000276"
     CDS             38353..39627
                     /gene="purD"
                     /locus_tag="IV247_000276"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAP8776374.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            39749..40663
                     /locus_tag="IV247_000277"
     CDS             39749..40663
                     /locus_tag="IV247_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAP8776375.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(40796..42361)
                     /locus_tag="IV247_000278"
     CDS             complement(40796..42361)
                     /locus_tag="IV247_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP8776376.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(42354..43805)
                     /locus_tag="IV247_000279"
     CDS             complement(42354..43805)
                     /locus_tag="IV247_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8776377.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIRESKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(43819..44835)
                     /locus_tag="IV247_000280"
     CDS             complement(43819..44835)
                     /locus_tag="IV247_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8776378.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            45055..46143
                     /locus_tag="IV247_000281"
     CDS             45055..46143
                     /locus_tag="IV247_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP8776379.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            46197..47723
                     /locus_tag="IV247_000282"
     CDS             46197..47723
                     /locus_tag="IV247_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAP8776380.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            47725..48930
                     /locus_tag="IV247_000283"
     CDS             47725..48930
                     /locus_tag="IV247_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8776381.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            49187..50164
                     /gene="guaC"
                     /locus_tag="IV247_000284"
     CDS             49187..50164
                     /gene="guaC"
                     /locus_tag="IV247_000284"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAP8776382.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            50428..51852
                     /locus_tag="IV247_000285"
     CDS             50428..51852
                     /locus_tag="IV247_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP8776383.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            52313..53422
                     /gene="pdhA"
                     /locus_tag="IV247_000286"
     CDS             52313..53422
                     /gene="pdhA"
                     /locus_tag="IV247_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAP8776384.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            53425..54402
                     /locus_tag="IV247_000287"
     CDS             53425..54402
                     /locus_tag="IV247_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAP8776385.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            54427..56070
                     /locus_tag="IV247_000288"
     CDS             54427..56070
                     /locus_tag="IV247_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAP8776386.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            56078..57484
                     /gene="lpdA"
                     /locus_tag="IV247_000289"
     CDS             56078..57484
                     /gene="lpdA"
                     /locus_tag="IV247_000289"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAP8776387.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            57646..57921
                     /locus_tag="IV247_000290"
     CDS             57646..57921
                     /locus_tag="IV247_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAP8776388.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            57927..58694
                     /locus_tag="IV247_000291"
     CDS             57927..58694
                     /locus_tag="IV247_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAP8776389.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            58862..60691
                     /gene="typA"
                     /locus_tag="IV247_000292"
     CDS             58862..60691
                     /gene="typA"
                     /locus_tag="IV247_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAP8776390.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            60952..62724
                     /gene="recQ"
                     /locus_tag="IV247_000293"
     CDS             60952..62724
                     /gene="recQ"
                     /locus_tag="IV247_000293"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAP8776391.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            62838..63140
                     /locus_tag="IV247_000294"
     CDS             62838..63140
                     /locus_tag="IV247_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAP8776392.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            63143..64306
                     /locus_tag="IV247_000295"
     CDS             63143..64306
                     /locus_tag="IV247_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP8776393.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            64332..67760
                     /locus_tag="IV247_000296"
     CDS             64332..67760
                     /locus_tag="IV247_000296"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAP8776394.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            67781..68905
                     /locus_tag="IV247_000297"
     CDS             67781..68905
                     /locus_tag="IV247_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8776395.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            68895..69332
                     /locus_tag="IV247_000298"
     CDS             68895..69332
                     /locus_tag="IV247_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP8776396.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            69316..69654
                     /locus_tag="IV247_000299"
     CDS             69316..69654
                     /locus_tag="IV247_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAP8776397.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            69773..70333
                     /gene="rsmD"
                     /locus_tag="IV247_000300"
     CDS             69773..70333
                     /gene="rsmD"
                     /locus_tag="IV247_000300"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAP8776398.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            70330..70821
                     /gene="coaD"
                     /locus_tag="IV247_000301"
     CDS             70330..70821
                     /gene="coaD"
                     /locus_tag="IV247_000301"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAP8776399.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            70811..71854
                     /locus_tag="IV247_000302"
     CDS             70811..71854
                     /locus_tag="IV247_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP8776400.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            72013..72741
                     /locus_tag="IV247_000303"
     CDS             72013..72741
                     /locus_tag="IV247_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAP8776401.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            72857..73102
                     /locus_tag="IV247_000304"
     CDS             72857..73102
                     /locus_tag="IV247_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP8776402.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            73099..73317
                     /locus_tag="IV247_000305"
     CDS             73099..73317
                     /locus_tag="IV247_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAP8776403.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            73453..73992
                     /locus_tag="IV247_000306"
     CDS             73453..73992
                     /locus_tag="IV247_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAP8776404.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            73985..76258
                     /locus_tag="IV247_000307"
     CDS             73985..76258
                     /locus_tag="IV247_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAP8776405.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            76296..77342
                     /gene="holA"
                     /locus_tag="IV247_000308"
     CDS             76296..77342
                     /gene="holA"
                     /locus_tag="IV247_000308"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAP8776406.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(77431..77685)
                     /locus_tag="IV247_000309"
     CDS             complement(77431..77685)
                     /locus_tag="IV247_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAP8776407.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(78085..78354)
                     /locus_tag="IV247_000310"
     CDS             complement(78085..78354)
                     /locus_tag="IV247_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8776408.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(78824..79279)
                     /locus_tag="IV247_000311"
     CDS             complement(78824..79279)
                     /locus_tag="IV247_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAP8776409.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(79417..81345)
                     /locus_tag="IV247_000312"
     CDS             complement(79417..81345)
                     /locus_tag="IV247_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP8776410.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(81542..82375)
                     /locus_tag="IV247_000313"
     CDS             complement(81542..82375)
                     /locus_tag="IV247_000313"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAP8776411.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(82567..83271)
                     /locus_tag="IV247_000314"
     CDS             complement(82567..83271)
                     /locus_tag="IV247_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP8776412.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(83294..83920)
                     /locus_tag="IV247_000315"
     CDS             complement(83294..83920)
                     /locus_tag="IV247_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP8776413.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            84246..86621
                     /locus_tag="IV247_000316"
     CDS             84246..86621
                     /locus_tag="IV247_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAP8776414.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(86645..87136)
                     /locus_tag="IV247_000317"
     CDS             complement(86645..87136)
                     /locus_tag="IV247_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAP8776415.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            87323..88243
                     /locus_tag="IV247_000318"
     CDS             87323..88243
                     /locus_tag="IV247_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8776416.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
BASE COUNT        28619 a        15250 c        18966 g        25479 t
ORIGIN      
        1 ttttagacat gaatatctta tttttattgc tatttttctt aattttcctt ctaacatata
       61 ggaaatgtca aaactattat gtgcgtatat tgactgcatt accgtttttc ttaaacctga
      121 ttatttattt tggtaataca atgggtcaaa gctttactta cgtcaatgga aataaaaggg
      181 caatgatttg gagtagtagt aacctgaata atctctttac agagttagga acgaaactgt
      241 cactttttta tccaggtact tggatagcta ctttagtagt cttggcatta ttactatgtt
      301 taattgttgg aatttattta agttttgaca ataagaaaac ttctattttt ttagttattt
      361 tgatgataat gggattttgt tcaaggttaa ttatgggatt ttctcccaca gtatgggctt
      421 ccggaatgcg gacgtactat atactttatg ttgttatcgc catcttagta ctaatggcag
      481 ttaaagagct aatgaaaagc atgagtgtac aaaaaaatga atttatgcaa tttggattaa
      541 ccgtgcttgg tatttgtacc tttataataa cggtgataaa cagataatat acaaaaaatc
      601 cctcgcttta gtattaggtg agggatttta ctcgtttttc gattaattgt tttaacactt
      661 gcaagtttct acgagtagct tgtttccgac cttcatatac tcacctcagg ctgtttacat
      721 gattgtacgt tctttttgta aaggatacag tgatatgtat ataatggcat aaagaaacgt
      781 tcaaacaaaa aaactcgtga agcaaaatta acttcacgag ttgtagtgca gatgatggga
      841 ctcgaaccca cacgagctaa cgctcacatg accctcaatc atgcttgtct gccaattcca
      901 acacatctgc gtaataataa cacactaatg atagcgaggt tttacatagt tgtcaagctc
      961 aaaaatggga ggacttatta agatggagaa atatgacgga gaattttcag gactgggaat
     1021 gatcttaggt gtattgattg gacttgcatt cggacgtttt ttgcttggtt ttatgctagg
     1081 aatcatttgt ggaattgcaa tggattgggc agcgaatcta tggaatgact atcatgacaa
     1141 ttaagttctc aacgcttgtt tgtgtttttt taatacttac gtttttccta aataagtggc
     1201 aaaattttct aggatggagt atgatattga aatagtaaat aacaaaaaat ttggaaagtt
     1261 ctaacttttt gaaatgaggg attggctaag tgatgagaga atttgaaaaa tccaacaaat
     1321 tagaaggtgt cagttacgat gttcgcggtc ccgttctaga agaagcggag cggatgcaag
     1381 aagagggcat tagtatttta aaattgaaca cgggaaatcc cgcacctttt ggatttgaag
     1441 caccaaacga agtcattcgt gatttgatca tgaatgcccg tgactcagaa ggctactcag
     1501 attccaaggg gatcttttct gctagaaaag caattgagca atattgccaa ctgaaacatt
     1561 ttccgaatgt gacgatcaat gatatctaca ccggcaacgg agtgagcgaa ttgatcacga
     1621 tgtgtatgca ggggttgctg gacaatggag atgaagtact ggttccaatg ccggactatc
     1681 cgttgtggac ggcttctatt gcattagctg gaggcacacc tgttcattat atttgcgatg
     1741 aagaggcaga atggtatcct gatattgatg atatcaaatc aaaaataacg agtcggacaa
     1801 aagctattgt tatcattaat cctaataatc ctactggggc actttatcca aaagaattat
     1861 tagaagaaat cgttgaagta gctcgacaaa ataacctgat tatttattcc gacgaaatct
     1921 atgatcgctt agtgatggat ggtctagaac atattccgat tgctacatta gcgcctgatt
     1981 tgttcgttgt tacgctgaac ggtctatcca aatctcatcg tgtagcagga tttcgagtag
     2041 gctggatggt cctaagtgga gataagtcac acgtcaaagg ctatatagaa gggctgaata
     2101 tgttgtcctc catgcgtttg tgttccaatg tattgtctca gcaaattatc caaaccgcgc
     2161 taggcggtta tcaaagtgtc gatgagctgc tcctgccagg cggaaggatc tatgaacaaa
     2221 gagaatatat ctataatgca atcaatgata ttcctggtct ttcagccgta aaaccaaaag
     2281 ctgcttttta tatattccca aaaattgata caaagcgatt caatattttg gatgatgaaa
     2341 aattcgtttt ggacttttta catgaacatc atattctact tgtacatggt ggtggattta
     2401 attggaacca gccagatcat tttcgaatcg tttatcttcc taaaatggat gatttgaaac
     2461 aaacagctaa gaaaatgaga gaattcctag caacctatcg ccaaaaatga ttttaaaaac
     2521 caaatttttc agtaggaggg ccacagacaa acacactgag gctctctttc tagtggagtt
     2581 ttcttaaata actaaagtat attatttagt tgcaagtctt ttcttgaagt ttgcgacttc
     2641 ctaaagtaaa atacgaatag aatgcgaata cgcatatttt ggggaggata cttatggaaa
     2701 cgttaatcga gtcctttgga acgatcatta aagagattcg aaaagaacaa aaaatgacgc
     2761 aaaaaatgct ttcgcatgat atttgttcac aaagtgtatt aagtcggatc gaaaataatg
     2821 aggaactgcc gaatgtattg gtgatgatgc agatttgtca acggctaggg gtaacgattg
     2881 atcatgtgat gaatctctct cgaaaaacta ctcggacaaa cgataaatta tttgaactgt
     2941 tggatagttg tttccaaaaa cgtgattatc ggaagttaga acaagtacta aaatcttctg
     3001 atatttctga aaatctgtat caagcaactg actttcaacg ctattattat tatctcggtg
     3061 tttgcgaatt cacgttgcgt caaaatatct ctcagtccct ttattatttg aaagaagcat
     3121 taagctactc tagccaaaaa gaaagaatcc atgtttctga cacagaaata caactgatta
     3181 gttgtatcgg aaaaatatat ggtgtggcag gaaaaacggc tgaagcacgc tactatttga
     3241 gtcgaagcat ccaactgctg catcaatcag ctgatgaacg atcaaaaagt gaattttcgc
     3301 aaatttttta taattatgga aatttcttat tccatcaaaa tgaaatcgac gaagcgttag
     3361 aacaggtgaa tcaaggaatc tattgggctc aagagaagaa tagctactat tacctcaatg
     3421 atttatttgt gcttaaaagc ttaatataca agcgtaaaga agaaccagaa agagcagcat
     3481 tctatgaagg cttagcacaa gctgtgaaac aaatttcaaa aaatttgtaa atggtgtaga
     3541 ccattcttga cttcttctct aaataagagt actttagagg tagaaaaaca aagggagaga
     3601 atagaatgag aacttttatc tatgctgaca aattcttttt agcgtcaggt gtcaaacatg
     3661 caggctatct tgaaattacc gatggacttt ttggtgaata caccaaagaa ctacctgagg
     3721 gagaagtcaa catcattgac caaagcggca aatggattgc tcctggtttg gtagatacac
     3781 atattcacgg ctatatgaat catgatgtaa tggataatga tgcagaaggc atcaaagtga
     3841 tgtctgaagg tctgttatct tgtggagtga cttcattttt accaacaaca ttgacgtcaa
     3901 gcaaagaacg tttaaaagat gtagctgaga caatcggcaa aatgtatcag gaagcaccag
     3961 gagctaaaat caaaggaatc tattttgaag gtcctttctt cacagaagaa cacaaagggg
     4021 cacaaaaccc aagttatttc ggggatcctg atttggatac tttccatgaa tggcaagaag
     4081 cttcaggtgg tttaatcaaa aaaattgctt tagcaccgga acgtcaaggt gtagaagagt
     4141 ttgtcaaaca agtgactgaa gaaggagtag ttgtttctct aggacacagt aatgctacac
     4201 tagaagaagc gcaaaaagct gttgaagcag gcgcaagtgt atttgtccat gctttcaatg
     4261 gtatgcgtgg attgaatcat cgcgaaccag gtatggtggg tgcgttatta tctttagaac
     4321 atgtcttttc tgaattgatt tgtgatggcc atcatgtaca tccaaatgct gcgggaatcc
     4381 taatggaaaa agcaggacat gatcatgtcg cattaattac agactgtatg atggccggtg
     4441 ggatgcctga tggaaattac aatttaggcg aatttccagt agtagtaaaa gaaggaacag
     4501 cacgtctaga atctggcaac ttagctggaa gtatattgaa attgaaagaa gcaattaaaa
     4561 atgttgtaga ctggggatta gcaacaccag aacaagcagt catgatggct agtctgattc
     4621 cagcaatcag ctgtaaaatc gatgatacat gtggaaaaat cgctcaagga cgcgatgcag
     4681 actttatcgt attgaaccct gatatgacat tggaagcaac ttatctagat ggcgtggaac
     4741 gtttccacgc ataatataat ttaagttaac cttaaaacta aacttcttaa gtttggtttt
     4801 aaggtttttt ttgtgttcta gtaaaaatca ctttacataa gtttcaaaag tttctttaat
     4861 ttcctattgt tcaataagta acaatgtaaa gataaaatta aaaatgttaa tacgtattag
     4921 caaaataaaa gcttttttta ttgtgaataa atgaacataa ttaggaggag aaagatgaac
     4981 ttatttgaca tttttcgatc gactttgaca gatatgtcga ttgtcagtgc aatcacttca
     5041 acagttttta ttatattatt agggtttgtt tgtagaaaaa aggggatttt ttctgaatct
     5101 gttggaaaaa ttctatcgaa agtcgtctta acagtggctt tgccagcact ggcatttcga
     5161 gctttcatgc aagatatcaa tacagaatta ctgatgcaag gaatcaatgt cttgatttgg
     5221 ggattagtta tttatattat tcttattcta gtttcaaaac cttttttctt aagctataaa
     5281 ggggataagc aaacaacgtt gcgtgtcctg acgatctttg gttctacgac ctttttcgga
     5341 acgcctatcg tcagtgcgat ttatggtcct gtcggcgtga tgtattcctc aattttcaat
     5401 attggctacc gaatcttttt gtattcttat ggatacataa aaatgagtgg cttgaagatg
     5461 gaagctaaaa atatcaaagc gatgttcgtc aatccaatca ttatcgctac ttttgccggc
     5521 cttttcattt ggctgttcca agggtatctg ccacaagtgt cagttacagg tgcagaagga
     5581 gagataagcc aagtagcgtt tttgcgaatt gaccagactg caccgtggct atttcagccg
     5641 atggattatt tagctggttt aagttcacca ttggcgtggc tagcaatcgg cgcaacatta
     5701 ggtgaagtaa gcttcaaaaa agctgcatct gaaaaaactt catggtatta cagtctagtc
     5761 aaagtggtat ttgtacctgc attgaatatc gtgattctag cagttttgac ggcaacgaat
     5821 attctaccag tcaattttga agcactggca acaatcgtta tcatgatggc tacaccagct
     5881 gcaacagtgg cagcagccta tgctatcagt tttgataggg aggcactttt agcctccaat
     5941 gcatccttac tgtcaactat ttttgctgtg gtactgacac ctgtatggat cgttgtatta
     6001 caaatgattc atggcatagg tttattttca taagaaatgg aggaagaaag tatgagaatc
     6061 gcatgttacg gtgttcgtcc gaacgagata gatttttttg aaaaattgaa tatttaccat
     6121 tatgatctca gtctttatga agaactgttg acacatgaca atatcgtgtc agcaaaggac
     6181 catgatgctg tattgttgag gggaaattgt atagcagatg agaccaatct tgcaaaaatg
     6241 caggaatatg ggattcgtta tgtttttaca cggactgtgg gagtcaatca tatcgatcta
     6301 caagcggcag ctgattttgg tatgacagta gcccgtgtac catcgtattc gccaaatgcg
     6361 atagcagagt tatctttgac ttttgccatg atgcttttga gaaatactgc gtatacgacg
     6421 atccgaacat ctttcaaaga ttttcgagtg gatgagcaaa tgtttagtcg tgaaatcaga
     6481 aattgcacag tagggatcat aggaacaggc aggatagggt tgacggaagc tgttttgttt
     6541 aagggactag gagccaaagt gatcggctac gatattcatc agacagaagc tgctaaaaaa
     6601 gtattgacgt tccactcgct tgaagagtta ttagccaaaa gcgatattgt cagtattcat
     6661 gtacctcata tcccaggtga aaacgatcaa atgatcaatg aaggattttt agctcaaatg
     6721 aaaaaaggta gcatcttgat taatacagcc cgtggagagc ttcaagataa tgaagctatt
     6781 ttaagagctt tgaaaagcaa tcatttagct ggttttgcaa ccgatgtttt tgcaaatgaa
     6841 acagaggttt ttttccgatc cttcaagcca tgggagacga ttccagaccc tgctatccag
     6901 caattagtag aaatgtaccc tagagttctt gtaaccccgc atgtaggttc aaatacagat
     6961 gaggcattat caaatatgat ttcgacaagt ttcgagaact ttcgtgaaat aatagaaact
     7021 ggaaaaacga aaaatgaggt atctcttcct aaagcccgtc aattaagata agaaaacatg
     7081 gatattcgtc aataaaatgt ttaaaaatag tccaagtaaa cagttgttac ttctgtatac
     7141 ttggactatt ttgtatggga taattcgatg actcaacagt aatctgtgag ctttttccgc
     7201 ccattctttg tcaattcata acaggttagc ggccggccga tttccaaata tttgattctt
     7261 tcgttcaata tatcttgttc tactaaaaaa ttcaaatatt tcttgacgga tatacgtgac
     7321 agtgagcttt tttgagccag ttcttttgtt gaaaaaatag tatcttcttt tagaataaat
     7381 gcacttattt tttttaaagt cagttttgac agacctttag gcaaatcgat gaatgatggt
     7441 atttcgtgaa tgggagtggt gtggttgaat acttgatcga tcaatttctg atcggctttt
     7501 tcagtcgttt ggattgtctg agagaaagca agatactttt cgatcgccag ctgaaaacgt
     7561 tgcaaagtga acggtttgat caaataatct aaggcaccgt agcggatcac attctttatg
     7621 gtttcgatat catctgcagc agtgatcaaa atcactggga tatcatttcc ttgctgtcta
     7681 agccaagata aaaaatggat accactttcg ccaggcatgt atacatcaag cagcagcaga
     7741 tctgcgtttt tttgtctgaa atattttttt ccttcttcta catttctgaa actttcgatt
     7801 acgttcatat tagggatgct ttccacaaaa gtacgattca attctgcaac cattggatca
     7861 tcttcgatta tcaggacatt tgtcatcatt ttgatcttcc ttttttggta attctagata
     7921 gacgattgta ccgccatttg tttgtttgga aaattctaaa aatccaccgt ggcgtttgac
     7981 gattgaatgg atgagattca atccaaaccc acgattgatt cccttcgtcg aaaaacctag
     8041 cgtaaatatt ttttctgaga tttcttctga tatcccgaca ccgtcatcgc agacggtcac
     8101 gatgaatact tcagcttctg tgtcatagtt taaatgaatc cggacctgtc cgtttctttc
     8161 atgtttttgt agcacggctt cttttgcatt atcaagtaag ttccctaaca cttgcaaaag
     8221 tgaatggatg gagtcatcca tctccatttt agggataaaa gaatcgcttt ctaacagcat
     8281 ttcaacagat tgttctttgg cttcgtttat tttgcctaat aaaaaaccgg caattgcagg
     8341 tactttgatc agacttgaga cttggccaat ctctttttta taattttgat ttagctgctg
     8401 gataaaatgg gtgacttctt catacttctt caaatcaatc aaaccagaaa tgacatgaag
     8461 tttattcatg aattcatggg tttgagctct catggagttt atatattgtt tcgtcccccc
     8521 aagctcctcc attaatttca ttacttctga tcgatttcgt aaagttgcga ctgctcctaa
     8581 aaaacggtca ttgctaaaaa gaggtgtaac gctagaaata gtttctatcc catttaaata
     8641 catggattga gtccgaacgg tcgtttgatg ctcaatcgtc tctttaaaca aaacgtcata
     8701 tagattatca ctgatttttt tcctacttcg aaatccaata gctgctctgt ttggtagaaa
     8761 atgttttttg caggtgtatt gatcaagagg atagttttat cagaagagat ggctaagatt
     8821 ccttcatcta cttcattttc gataatcgtt ttttcactta actgttctgc tatttcttgt
     8881 ggttcaagtc caagcagtac ttttttcatt ttttgggcaa gaaaaacggc gcctatgatg
     8941 cctacgctaa gtccaataat caatgaaaaa acaatcattt tttgtccatc tttgacttga
     9001 ttgtcaatcg tgttgtttgt taagccaaca caaactgcac caataatctc ttgatcataa
     9061 taaataggga cgaaatagcg gaagccgaac cctaacattc cttctttttc agagaaaaat
     9121 tcttgtcctc caatcacttt tttcacatct tcatgacttg agaaaggaag accgatgtaa
     9181 cttgggtctg cgtgagaata tcgaatgcct tcagggtcca tgacaacgat aaaatctaaa
     9241 ttcgatgcaa tcatgatttc atttgttatt ccttgaatcg catgtgtcgg gtcaccagtc
     9301 tgcagctttt tttggatgag tttatcattg gcaacgattt tagcaatgct agagatttta
     9361 tctgacacac tagatcggtt catcgaaaga ataaaattcc tgatcaatat ggcagaaaca
     9421 atcaaagaaa cgaatgtgga gaaacaaacg acaaggatga tcgtgttctg gagctttaat
     9481 ggttttttct taaaaaaagg aaacataggc tacctcgaaa gaatttattg tgaaaatttt
     9541 aacatgaaaa caaaaattta acaaattgtc aacgataaac acaaccttcc tataaaaaac
     9601 accactgagt cggtaaaaga agctatacag aattttcaaa cattggtatg atagacgtag
     9661 aaatggaagg aggcgtgtat atgatagggg gaatcgcact tgagacgatt tattggtata
     9721 cattgctgat ttgtgcagga ttggcagtcc tattgattat ttttggagat gtattcgatt
     9781 ttgatggccc gcttgatccg atgcttatta tcccttggtt aactttcaca agcttatttg
     9841 gttttctagg tgagcgttta acagatcaat catctttatt tttatttatt gctagtggag
     9901 gcatatcgat agtgttggta tttttgctta atttctatat attgatgccg atgaaacatg
     9961 ccgaatcaac gatgtcgtct tctgaaaaaa ctctggaagg acaagtagct gttgtgatca
    10021 caccgatccc tgtacgaggt atgggagaaa ttcagttgaa aagtgttacc ggttccatta
    10081 gtcgtccagc ttgtttttac agcccccaag aacagacagc tgagcgggga acgaaagtat
    10141 tgatcattga gatcaaggaa agaatctgtt atgtcatgcc ttatgaaagc atgataaaag
    10201 cttaaaggag cgtgtgttaa atggaaatac cagttgtttt tatcgcgatc gcaatcgcag
    10261 ctttgatttt actgatgttg ctgatcgtct ttgtagcgaa gtaccagaca gcaaaaccgg
    10321 atgaagcgtt gatcatcagt ggaagttatt taggaagcaa aaatgtgcac attgatgaaa
    10381 gcaataacaa aatcaaaatt gtgcgtggag gtggcgcatt cgtcttgcca gtttttcaac
    10441 gttcgaatcg gatcagtcta ttatcgagta aattggacgt ctccacccct gaagtgtata
    10501 cggaacaagg cgtaccggtg atgtgtgatg gtacttcgat catcaaaatc ggttcatctg
    10561 tagaagagat tgctacagca gcagaacagt ttttaggcaa gacaagagaa gaactggaaa
    10621 atgaagcaag agaagtatta gaaggacact tgcgctcgat tcttggttct atgactgtag
    10681 aagaaatcta tcaaaatcgc gataagttca gtcaaagcgt acaagaagtc gcttctgtag
    10741 accttgcaaa aatggggttg atcatcgttt ctttcactat caaagaggtc agagacaaaa
    10801 acggttatct tgactcttta gggaaaccaa gaatcgccca agtcaaaaga gatgcagata
    10861 tagcagaagc ggaagctcta aaagaaacgc gtatcaaaaa agctcaagca gaaaaagaat
    10921 cacaagcagc tgaattacag cgacaaacag agatcgcaga atcattaaaa gaaaaagaac
    10981 tgaaattagc aacctacaaa caagaacaag atgttgctaa agcaaaagca gaccaagcct
    11041 acaatttaga aagtgcccga gcacaacaac aagtaatcga acaggaaatg cagattaaaa
    11101 tcgtagaacg tcaaaaacaa atcgaactgg aagaaaaaga aatcacacgt cgtgaaaagc
    11161 aatatgattc ggaagtgaag aaaaaagcag atgccgatcg gtacgctaaa gaacaagaag
    11221 cacaagctca aaaagtcaaa gaagtgactg aagccgaagc agaacgattc cgtgtggaag
    11281 cactggcaga agcggaagcc aataagacac gtttagctgg acaagcagaa gccgaagcag
    11341 cactagcaaa aggaaaagca gaagccgaag caaaacaaaa gatcgccaat gccttcaagg
    11401 aatatggcga agcagctgtc ttgagtatgg ttatcgatat gctaccgcaa ttgatgcgcg
    11461 aagcagcaca gccgctagga aatatcgaaa aaatctctgt tgtcgataca ggaagcagtt
    11521 caggagaaac cagtggagcg aaccgagtga caaattatgc cacaaatttg ctttcttcta
    11581 ctcaagaaac actaaaagcg acaacaggac ttgacttgaa ggaaatcgta gagaatttgt
    11641 cgacacgtgg gactactagc caagtaaata tgtatgaagc gccaaaagag gcagaacaaa
    11701 actcgatgaa ggaagaataa agtagtacac ttcaccgttc aatcaaaaat ggaggaagac
    11761 tttttaaagt tcttctctcc atttttttat tttattgcaa aagaaaatta gtcctcgcta
    11821 tttttctttt gtttttggat cttagcttct atctcttgta aatttgttct tagcaatcgt
    11881 tctttctttt ggagttcgac caaatcttct gatagttctt ccttcttttc tgtttcagga
    11941 ggacgaagaa agaaattgat accgattgca atgaatgtcc ctataaaagt atctagtaca
    12001 cgttggatag catacaaagc ggattcgcct tgagggatgc tcaacgcaat cagcaacaac
    12061 gtagctattg cggaaatgat accggaatta ttgttaatac catctgacac gacgatcaca
    12121 ataataacta gaataggaag caagatcaat tctaccaaaa aatcattgtg aaaatagctt
    12181 ttgacaaaaa aataaatgat agctaatgta ccacctaatg tattgccgat gatcctagac
    12241 tttccaaaag aaacggttgt agtcaagtcc tgacgcaaag aaaaaacagc agataatgca
    12301 gcaatcatcg ggattcctcg ttgagtcaaa tgaaaaaaca agatacataa aaaaacagct
    12361 aaggcacttt tgatcgtacg catacctagt cgaaaacggc caaattgcat agagattctc
    12421 ctttttggtc tttttactat catacctaaa cttttgtctg atagctagtt tttcattatt
    12481 caagtataat taaaataaaa aagggggaag taaatgcgta taggaatgcg aacagtcaaa
    12541 acagtgatca gtgtcttctt ttcgatggtc acagcgggta gcttatcgct gttgtattgg
    12601 ccagctgcag ggatcattgc attattaagt gtaggaaata ccaagcgctc tacattgcgt
    12661 acgggcatga atcgaatcgc agcctttata ttagctacgg caacagcact gttcagtttt
    12721 tttgctgttg ggtatactat ttttggcttc agtctttttt tattcctgtt catcccgctt
    12781 gcctctcgct tcaaattaac cgaagggata gtagtaaatt cggttttggt cactcattat
    12841 ttagtggagg aaaacatgtc ttggcagctg atcggcaatg aagcgggatt aatggcgatt
    12901 ggattagtat ttgctttgct tgcaaatgtt tacatgccag atcgtacgaa gcagctgaag
    12961 gaaaaccaaa tacagatcga aaaagaattt cgaaatattt tgagacaaat ggcagaattt
    13021 cttttatcag aaaataaaga agatgtgcag atcaaatgcg aacatttact gacattcatt
    13081 cgcgaaagcc aagaagatgc cagagaacat caagaaaact actggctcag acagccgcta
    13141 tactacgaaa cttacttctc tatgagaaga gcacaggcca atgtgattaa agacatgctg
    13201 gaaaatctag agagaatcca gcaaccagct tattatggaa aacatatcta tggtttattg
    13261 atttatactg cagaaacatt ttcagagtca aatgatggca gacaaatttt aacacgtatt
    13321 gaagaagttt atgtattgta tcggcaaatg cctctgccga ctagtcgccc tgaattcgag
    13381 gatcgagcag aattgttcca gtttttgcag tcttttaaaa gttttattga aatcaaggca
    13441 gaattctccc aacaaaaaat ccaaaaatag aggttgtgaa ttcagctaat attcaggtca
    13501 aaaagctgat ttcacaacct cttctttaaa cggtgttctc ctgtcaactc ctcggtcaca
    13561 agtcacaata ttcaacaaac atgagaagct gttttttgaa attgctcctt gtgactcgga
    13621 gctgaacggt aggctcacaa cctctttctt aaacggtgtt caaaagcttt cctctgcggt
    13681 aataagtcca gccaaacgaa aaataggagg agctattttt gtttggctgt tcttattact
    13741 tgatgcagaa cagctttttc acaacctttt aaaattacat atttttccat agaacttgca
    13801 tttgatccaa gatttcgatg acatcttttg tcaagaagag agagcgattg ggcaagtcac
    13861 cttcaatcat gtgtaccata ttctttattt cgtaattcat tgcttcagat gtattcccgc
    13921 tttcgatgaa ttcctttgta ccatctgtaa ataagatctc cgcacgatca gcacgcgggt
    13981 aatcagcaat cgtcacataa ccgttctcaa aggcaacgat accgactttt ggcatttttg
    14041 cctgaaaagt caagctgaca gcagcaagtt cattttcttt gttacgcaag atagtgacgg
    14101 attgttcgtc cacgcctgtt tcaaaaggaa ccattgtaga ggcaactact tcaggttgag
    14161 agctcaaaaa gaaacgagcg aaagatacag catacgtacc gatatccaac agagctcctc
    14221 cagctaattc tggattgaag aaccggtttt tagggtctgg ttctttgtaa ctgccgaaag
    14281 gtgcttggat catttttaaa gcccccagtt ttcctgtatc cattattgag cgtaactgct
    14341 gataaagtgg catgttgaag attgtcatcg cttctgctaa gatcaaattc ttttcttctg
    14401 ctaagcgcat agcgtcagcc agttcttttt tattcatcgt gatggctttt tcacatagaa
    14461 cgtgtttgcc tgcttccaat gctgccaaga tatggtcgat atgctgtcta tttggtacag
    14521 caatgtacac gatgtcgatt tcagcatcgt ctaacatttc ttgatagctt ccgtaagctt
    14581 ttggaatatt gtaacggtga gcgaagtttt ccgctttttc tgaagttctg gaagcaactg
    14641 ctgttagttc gctcgtctct tgattgaagc tttcggcaaa ctcatgtgcg atggtaccaa
    14701 gaccaacgat tccccattga tattttttca tctgaaaacg ctccttcata gttggattga
    14761 gtttcttgtc tgtctattat tataacaaaa atcacgaaga ataaagagga gaggacttga
    14821 aacattaaaa aaaatgaaag cagcgattta agaggtgctt tttgatcaaa acttaagtag
    14881 aatagttaaa aggaggggat aaacgtgcat caagtatata ttgatcttat tatgagtgta
    14941 ttattagaac agtttgaaac tgaaaaaaat tttttagatg aatacttgct gatcagtcca
    15001 gaacaatggc aaaaatggaa agatggcgag aaaaatctgc catctgaagc gatgcagaaa
    15061 gtaaaaaatc ttttcactga ttatgaatgg atgctaacgc aaaagatttt gcgtcaaacg
    15121 attatttttc ccgaaaaacg aaacgttgcc gttgcagagt acaagcaaat caaaacacgc
    15181 atcgcgcaaa aatggcttag cagcaaagca gcaaaagtcg agctgatccc tttgaaaaat
    15241 aatcagcagg ctggtagata ccttgacttg aaagtaagta ttcaatatga tgaatggggc
    15301 tatgatgata ttttgaattt tcgtctgcca gcagatgttc aaaaacagat cgaaggagaa
    15361 aaaatcgaac ttctcgattg ggtgaatgaa aatctggaag aaacttacgt tcatgaggcg
    15421 aatggggaac gtgaagaaca acgaaaggat ttttcaagtg aataaatttt taggatttgg
    15481 agcagttgtg attatgatcg cagcaatggg ctatgttatt tttagcgatg atgattcgca
    15541 tgttcaagcc ggcaatcaag cagtcgaacg aacaagcacc aaaacaactg ctagtaaaga
    15601 aaagaacagt caaacaacca gcagcaaggt aaaggacaaa gaaacaaaag atcgtacgtc
    15661 caaacaattg ccagatgtta aaacagacga ttggaagcta gttttggttg gtccagatca
    15721 caaattagaa aaagaaattg atgagtctac tcaattggca tcattaagta acggatactt
    15781 gatcgacaaa cgcattgctc cagaatatga agcatttgcc aaggctgctg aagcagctgg
    15841 atttccatta gtgatggtct cagcttaccg gtctgttatc atcccaacaa caagttttcg
    15901 aacaaaatgt ccaagatgtg atgagtcgtc aaggtgtatc agaagaggag gcgacaaaga
    15961 ttactaaaga aacgattacg gagccaggat acagcgaaca ccacacaggt ctggcagtcg
    16021 atgtcgtcga tcaaaactgg tacaacagtt atccttctac tgtattggat gcaagttacg
    16081 gagatcagcc tggagctaaa tggatcgcag ataatgctgc gcagtatgga tttatcgttc
    16141 gttatccaaa aggacacgaa gatataacta agatcacata tgaaccttgg cacttacgtt
    16201 atgtcggtaa agaaaacgct gagtatatca ccaaacacca attgacgcta gaagaatatc
    16261 tgaatgaatt aaatgaaaag taggaattga tcatggcgaa aaaaaagtgg aaaaagcaaa
    16321 agcgcaagaa tatccgttgg ccagcagtgg ttgccggtgt agcagtgata ctcttagcct
    16381 ttgttttttc cttgaagaat ctgtcttctc cttttactga taatcagcaa gaaaatgaag
    16441 cgcaatccca tcagcaattt attgaacggc ttgttccgca tgcccaagaa ctacaagatg
    16501 gttatggagt gcttcctagt atcattttag gacaagctat attagaatca aattggggaa
    16561 aaagtacctt ggctagcaaa tacaataatc ttttcgggat caaggcttat ggggatcaga
    16621 agaaagtaag cctggaaaca aaggaatttg ttaacgaaga atggataact atccaaggag
    16681 acttcaaagt gtatgattca tgggaacagt ccatggatga ccatacacaa ctgttcgtcc
    16741 aaggagtaga ttggaatcct gcgctgtatg aaaaagtgat tacggccacc aactatcagg
    16801 aagccgcaca agcattgcaa gatgcaggtt atgcgacaga ccccggttat gcgcaaaaaa
    16861 tcatccaggt catcgagacg tatcaattga atcagtatga ccggagatag caaaggagaa
    16921 gtggctgatg gcagaacaat ttacgccaga aatcatgacg gaattgagaa aagatatcgt
    16981 aagagtaccg gaaatcattt tagaagcaag cgggatcaag atatttggaa aattgatccg
    17041 ttcgatcatc tttactacgg atattgcgat catacgtaat acaaatgcga atgcagtaat
    17101 tgctgtgtat ccttttacac ctcatccagc gattactaaa agcattattg aggcagctga
    17161 cattccagtt ttctccggtg taggtggtgg tttgacacaa ggtgctcgtt cttcctacat
    17221 gagcatgttc gcagaagcgc aaggttcgtt aggggtcgtt ttgaacggtc ctacgccttt
    17281 agagacagta aaagaagttt gtcgagtagt tgatattcca gttatcagta cggtaacgtc
    17341 gaaatataca gaaatagatg aaaagttagc agctggtatt tcgattatca atatcagtgc
    17401 aggtaaagaa actgcagaag cagtacgcca ttttcgtaaa aaatatcctg aacttcctat
    17461 cattgcgaca ggtggtccaa cggaagaaag tattgctgag accattcaag caggagcaaa
    17521 tgccatcaca tatacgccac caagtagcgg agagcttttt agtcgtaaaa tggaaaaata
    17581 tcgggaaact gagaaaaaga atcatgaata acaggtgaga atcaaaagac gaggaagagc
    17641 agatagtggc tcttcctcgt cttttgtact ccagtactat ttgttcagaa ttcaaaaaaa
    17701 agagaaattt ttttgttttt ttacctgtaa tgtacataag aagagttaaa accttttgtt
    17761 attattagca ttctaacaaa aaaatgtctt gatttattga agcgctttct atagaatgaa
    17821 aaacgaataa aaactaaaaa aggcacccga caatttagta gcagctaaat tgtcggggct
    17881 tgaaaagtca gcgggaactg accaatcaag ttgccatagt caatgcttgt gcatcgacgt
    17941 gtatattgta cctaatttca tacagaatta ctagttattt ttctgttttt tagggaagag
    18001 cgcacacgaa gaagcatgct tatttagcta tgtcaaggat attgggcagt tgtctgatat
    18061 ccttttttct ttgcgtaatt atcctcccat cattgaagca agaagtgttt ttagcgatga
    18121 aagaagaatc agcactatct aatttttctt gaaagtattg agttgttcat cctaggcaga
    18181 acgataaggc aagtcttggt ttttattccc cttgaaaaag atcgtttaat aaatatgttc
    18241 tgtataaaaa tacagtatat tgaaaaataa caatggaagt agaggtgcag agaatgaaga
    18301 aaagaatgag aatcatctta gctttaagtg ttatgttatt cttcttgacg gcttgtgggc
    18361 aagaagatga caaaaaaatc acgctgaaca tggcttggtg gggttcgcaa gtccgacacg
    18421 atgcaacagt aaaagtaatc gagctttatg aagaaaaaaa tccccatgtg acgatcgatt
    18481 atgaatttta tgattatgaa ggatattaca caaaattagg tacactagca gcatcagatg
    18541 atatctggga cattttccaa ctgaacaatc aatttcctca atacatcaat cagattgaac
    18601 cattagatga atatgtggag caagggataa ttgatatttc taatgcagat gaatcctatt
    18661 tagcaacgac gacttacgac gggcaattag ttggactgtc taacggggtg aattcgtttg
    18721 cgattgctta caataaaaat atatttgaac aattaaatat ccctgaacca gcttcaaatt
    18781 ggacatggga tgaatttgaa caaatcagct atcaaatcac tgaagaatta ggaatctatg
    18841 ctatttcaag attcgaagat tttatagctg gctgtattat tcaaatacca caagttgaga
    18901 aaggattgaa tttttataat cgggaagatc tatctaacag cttgggattt gaaaatccag
    18961 actatctaac agattttttc cagctgcgta aaaatctggt agacaaaggg gcccatccag
    19021 aaccaggtgg tggagctgca agtacgacgg atctgtcttt tcaggcggtt agggatagag
    19081 aagcggcaat ggtgtttcta tcaagtaacc agttgacaga aatattgtca ggggctcctg
    19141 aagatatgga gattcagttg attaatccac ctcgcagaaa agcagatgga gaatctggta
    19201 tccctttacg ttcttcgcaa atgctgagta tggcaaaaag ctcagcccat aaagaagaag
    19261 ctgctaagtt tattgatttc tttcaaaaca gtgaagaagc caatgaaatt ttaaaaggag
    19321 aacgtggtgt accaatcatg tcgaatattc gagaacagct cgctcgtgca gatgatgcaa
    19381 ctttgcaagc gacatttgat tatttagaaa tgatttccga tatggacaac ggcgaagtga
    19441 atgtctttga tagtgctttg aatccggaaa ttcaagacca atacaatctt ttagttgaaa
    19501 aagtcattta tgaagaagta acagcagaag aagcagcaga gagtctttat gagtttgcgt
    19561 caaacattct aggatagcag caggaggaag gcatgaaaaa agaaaaacta aaacggttct
    19621 tggatcaaga caatgtggtc ggttacgtat ttgcttctcc attcatccta ggatttctat
    19681 tgataacagt gataccgatg atcttgtcac tttattattc gttcaccgat tatcgtttgg
    19741 gaaatccagt ctcatggatc ggcttggata attatataaa gatgctgagt gatgcccgtt
    19801 ttttgaactc catccgagtc acttttctct atgtatttgc atctgtacct gtgaaactga
    19861 tctttggtct attgattgct tatcttttaa cgaggaatat caaaggaacg actttctttc
    19921 gttctttgta ttatgtgcct agtttgatcg gtggcagtat ttctatcgct ctagtttggc
    19981 gggagttgtt ttctaaaaac gggttagtga atattttcct ggaacgatta ggcgcacctg
    20041 cgctttcctg gtttagcgat caaaaactgg tcatgatccc tcttgttttg atgtctgctt
    20101 ggcaattcgg ttcggcaatg atcatctttg ctgcaggtat caaagagatc ccaggctcgt
    20161 attatgaagc ggcagagatc gatggagcga acaaatggca gacgttttac aagatcacgt
    20221 tacctcttct ttcgccgatc attttgtata acttggttat gcagacaatc gcggcgttta
    20281 tgacttttac gcaggcattt atcattaccc aaggtggtcc gaatgatgcc acgaactttt
    20341 atgctcttta tgtgtataac caagcttttt cttttaattc tatggggtat gctagtgcta
    20401 tttcgtggat catgctgttg attatgggag ctgtcacctt tctgattttc aaatcatcaa
    20461 aaaaatgggt cgttgtgtga atgagaggag gaagaaatga ataatacagt aacgaaatgg
    20521 tttcaatacg gttttatttt gctattaggt ctagtcatga tttatccggt cttatggatg
    20581 atcgcaggtt cttttaaaac ggatcaagag attatgagtg gttcgctgaa tttgattcca
    20641 gagacttttc aatgggaaaa ttacgcaaaa ggatgggcgg ggtttgctgg aatttcattt
    20701 tttactttct ttaggaattc gtttgtcatc tctgcagttt cgacgatagg gacagttctg
    20761 agctcgactt gtattgctta cgcattatcg agaatcgatt tcaaaggaaa aagattctgg
    20821 ttcgtcgtca tgttagtaac gatgatgatt cctgcacaag tgattttgat tccgcagttt
    20881 attatttaca atcggctgaa tttggttggg acgtatgtgc cgctgatttt accacatttt
    20941 tttggacaag catttttcat ttaccagatc atgcaattca tggtcagcat ccccaaagag
    21001 ttagatgaat cagcgattat tgacggatgc agcaagtata gtgtctttac caaaattatt
    21061 tttcctttgc tgaaaccttc gatcatcacg acgatcatca tccagtttta ttggaaatgg
    21121 gacgacttca tgggaccatt gatttattta aacaagccga gatcttacac ggtatcgatt
    21181 gccatcaaat tatttgcaga cgctggatct agtacgactt acgcgagtat gtttgcgatg
    21241 tctacgttgt cgctgctacc ggtttttctg attttcttat tcttcaataa atatcttgtc
    21301 caggggatca gtacaagcgg tctgaagggc tgaatatgaa aaagccaata gaagagatgt
    21361 ttctttacaa gctcaactac tatgtctgtc ggattttttt aaccagtctt atcagtaatg
    21421 ctcttttcgc tttagttcta ggatgtttgt tcactttagg attagtgctg tctgctcgtg
    21481 ttgtctatct gatttatttt ttgatgatgt atccttctta ttatctatct gtccattact
    21541 tggcagaaaa aaaggtatgt tggaggaaag gaaaaatcaa acggatgtgt ttcatcatta
    21601 cgttgtttca gctgtttgtc attatttttg cagttgattg gtatttatta gtgggaaaga
    21661 ttggagcttg gtatctgatt ccttttctat tgtgtctgtt gctttttgta tcccatagtt
    21721 tttttagtac gctaattgat atctgcaaag aagataaagc agatagtctt gtttatttga
    21781 tcaaaaggaa tatctggctg ttgtcaaaaa acgtcaaggg gtttctgtgg tatttatgcc
    21841 ttttgtatgg actgttctgg tgtatacagc atgatttttt actcactgca ttactgtatc
    21901 cagggctgcc atttgtcaca gtattttgct taaaagaaaa ggagaatgac tttaaatgaa
    21961 gaaaaaatat gtacaagtcg gacttggtgg gagagcgcgt tttttttatg aggcaattgc
    22021 ctctacgtat catcaaactt gtgaactagt cggtttttgt gatgtatctc aaacaaggat
    22081 ggattatgca aatcggatac ttgtgaacaa gtatggacag aaaaaagtaa agacatgtat
    22141 gccatcacaa tttgagcaac tactggatga cgtacggccg gctgaagtca ttattacgtc
    22201 agtagatcga acgcatcatg attatatcat tcgtgcaatg aaaaaaggat atgatgttat
    22261 ttgcgaaaaa cctctgacta tcgatgagga aaaagctcaa gaaataataa cggtccaaaa
    22321 acagacggga agaagcctgc gagtcacttt taattatcgt tatgcaccgt atcacacgaa
    22381 gataaaggaa ctgatagaaa gtgatgtgat tggtgatgtg ttttctatcc acttcgaatg
    22441 gctgttaaat acgcaacacg gggcagatta tttcagaaga tggcatcgaa ataagcacaa
    22501 tagcggcggg cttcttgttc ataaatctac ccatcatttt gatctagtga atttttggtt
    22561 gaacagccgt ccccaaaagg tatttgcatt cggtggatta aacttttatg ggatgcataa
    22621 tgcggagaaa cgcggagtag aaatatttta cagtcgatgc tatggaagcg aactagctaa
    22681 agctgatcca tttgctatag acctgtcagc caatgaggaa ttgcgagaaa tgtatttaga
    22741 agcagaaaaa gacagtggat atattcgtga ccgcagtgta ttttccgatg atatctctat
    22801 tgaagataca ctaggcgtaa cggtcaaata tgaaactggt gcgattttga cgtattcgct
    22861 aaacgcgtat atgccgtggg aaggattcaa tgtttctttc aatggcagca aaggacggat
    22921 cgaaatgaaa gtgatcgaaa agtcttacat caatgcaggt ggagataaaa aggatgaagg
    22981 agcagccgtt cttcagtcca tccatgtcta tccgatgttc ggtgagccat atgaggtaat
    23041 cgtcgaacaa gaagcgggcg gacatggcgg aggagatccg cgtttattag atgatctatt
    23101 tggtgaagaa aaagaagaag atccgtggaa tcgggcagca actcacgtag atggtatttt
    23161 gtcgattttg acaggaatcg cagcaaatca ttctattgct tcaggaaaag cagtagtgat
    23221 tgacgagctt gtcagcttaa aagaataaga atagatttac ggaaattact tcccgtagtt
    23281 tataaaatct tgccttattg ccgataggct gtcttttgaa cactatttat tcggaaaaag
    23341 gggctatgac atgctttttg tcatgccttc tttttatgtg ggctgctttt ttagatagga
    23401 gaaaataaaa ataactattt aaacccgaac aaaaaggaat aatgcattaa aaatcattag
    23461 attttacaaa aaaataagta aaccacgttg tcagtttcag aaagtagtgt tagactgttt
    23521 tcaatatttg aaacaacatt ttttattcaa atatggcaaa ctgccttcac gtgcaaaaca
    23581 gttgcaagaa aatgaattta cactattatt agttttacat acctacttat atatcgtcat
    23641 aatatggatg acagtttcta gccagtaccg taaatgctgg actataagta aaagattggc
    23701 tatttcaccc ttcttttctt atgataaagt ggagggtgtt tttctttaac agaaacatag
    23761 taggtttttg cacaaaagcg gttatttcgg tattaataaa tgataaagga gcaaataaag
    23821 tgaaagaatt agttgaacgt attcaaaaag acggacgtat attaggcgag ggcgtattaa
    23881 aagtcgacag cttcgtcact caccaagtag accctgtatt gatggaacaa atgggaaaac
    23941 ggtttgcaga agtatttaaa gaacaaggaa tcacgaaagt tgtgaccatt gaagcttctg
    24001 ggatcgcacc agctttattt gctgcaagag aattagatgt tccaatgatc ttcgctagaa
    24061 aagcaaaaag tttgacgatg gatgaagaat tattgacagc ttctgtttat tcttttacga
    24121 aacaagtgac aagtacgatt tctatttctc gtaaattttt atcttcagaa gacaaagtat
    24181 tgattatcga cgatttcttg gcaaacggac aagcggcaaa aggattgatc gaattatgcc
    24241 agcaagctgg agcgcaagta gaaggaatcg ggatcgtcat cgaaaagtct ttccaagatg
    24301 gacgtcagtt acttgaagaa atgggattga gagtcgtttc tcttgcgcgt attgcttcct
    24361 tgacgaatgg acaagttgag tttcttgagg aggacgcata atcgtggaaa aagaaacaca
    24421 aaatggaaaa gctgcggttt tagggctgca gcatctgcta gcgatgtatg caggagcagt
    24481 agcggtgcct cttttgatcg gtacaggact aggctttaat caagaacaga tgacttattt
    24541 gatttcaatt gatatcttta tgtgtggatt agcgacatta ttgcaattga cagttaaccg
    24601 atttttcgga attggcttgc ctgtcgtttt aggatgtgcg atccaagcag tagctccact
    24661 gatcttgatc ggaaccaatg aaggagtagg agctatctac ggatctatta tcgcttctgg
    24721 gatctttgtt gtgttagttt ctggtgtctt ttctaaaatt aaaaaactat tccctcctat
    24781 agtaacagga acggttatca ccgttattgg tttgactttg attcctgtag caatcgagaa
    24841 aatgggtgga ggtaatgcaa cagcagctga ttttggtgac aaaacaaatc tgctacttgc
    24901 atttgtgacg attctcctaa ttgtaggcgt gcaaatgctg gcaaaagggt tcattcgttc
    24961 aatcgcggta ttgattggtt tagtcggagg aagcatctta gctgctttca tgggaatgat
    25021 tgatctgagc gcgattggag atgcgccgtt gttccatgtt ccacaaccct tttattttgg
    25081 taaaccaact ttcgacgtat ggtcgatttt actaatgatc atcatttcca tggtcagcat
    25141 ggtagaatca acaggtgtat attttgcact tggtgacatt actgggaaaa atgttggaga
    25201 agaagaattg aaaaagggat atcgtgcaga aggacttgcc gttatcttag gcggtatttt
    25261 caatacattc ccttatactg gcttttctca aaatgtcgga ttggttcaac tttcgggaat
    25321 caaaacaaga aaacccatct atttctctgc attcttcttg attgttttag gattattccc
    25381 taaaatcggt gcgttagcac aaatcatccc tgagccggta cttggtggag gaatgttagt
    25441 catgttcggg atggtggcag tccaaggaat ccgtatgctg ttggaagttg atttcagcaa
    25501 tgataaaaat ctattgattg ttgctgtttc tgttggattt gggctgggtt tcaatgttac
    25561 gccgacatta ttcagtcaaa tgccgcaaac attgcaaatg ttcactggaa atggcatcgt
    25621 aatgagtagc attacggcta tcatattgaa tttgatcttt aatggattga aaaaagatga
    25681 agaaatattg gaagaagcct tcaagaagta atagagcaaa agaaaggttg tgaaatcagc
    25741 gttttgacct gattgttaga gaaacaaacg gaaaaatagt tcctcgtatt ttgccgtttg
    25801 tttgagctaa taccgagggt cagctgattg aacaccgttg aaggaagagg tcgtgagcct
    25861 gccgttcatc tccgagtcac aaggaacaat ttcaaaaaaa agcttctcat gtttgttgaa
    25921 tattgttcct tgtgaccgag gagttggcag gagaacaccg tttatccata aaaaggaatg
    25981 tgacctaact tttgtcacat tccttttttt tatctttaat aaacacgaac aataaaatgt
    26041 ttttctttgt taacattcga aatttctttg acatcaaaaa aagtgattgt taaaatatga
    26101 atgtagaaaa actgggagga aaaacaatga cagcaaaaat ctctgtgatt atggggagta
    26161 cttcagattg ggaaacaatg aaagaagcat gtcaaatatt ggatgaattc ggtgtgtctt
    26221 atgaaaaaaa ggtcgtttct gctcatcgaa ccccagattt gatgtttgaa tatgcacaaa
    26281 acgctcgaaa aaaaggaatc aaaatcatta ttgcaggagc agggggagcc gcgcatctgc
    26341 cgggtatggt cgcatcaaaa acaactttgc cggttatcgg tgtaccggtc caatcacgaa
    26401 cattaaatgg tttggattcg cttctttcga ttgtccaaat gcctggagga gtacctgtag
    26461 ctactacagc aatcggaaaa gcgggagcaa ttaatgctgg actacttgcg atacagatgc
    26521 tttccatgta tgatttagaa ttggaggcta aattggctga aagacgttca atgttggcta
    26581 aaactgttat agaaagtagt gatcaacttg gttaaacctt tgttgcctgg ttcaacgatc
    26641 gggatcgtcg gtggcggtca attaggtcgt atgatggcga ttagtgcaaa agaaatgggc
    26701 tttaaagtag gtgtgctgga tcctgtgagc gattgtccag ccgcacaagt tgcagattgg
    26761 catattgaaa gtgtctatga tgacaccttt gcgctgaaag agcttgccag aagaacagat
    26821 gtcattacct atgaattcga aaacgtcagt gtagaggcgt taaatgcgat tttacccatg
    26881 tcatttattc ctcaagggac agacttgctt gcgattacgc aagaccgcct gttagaaaaa
    26941 tcttttttgg aaacgaataa catcgtgatt gctccttatg caacgatcgt cagtccgacg
    27001 gatatccaag atgcgattga cgggatcggc tatccttgtg ttttgaaaac cactcgaggc
    27061 ggctatgatg gcaaggggca gtatgttttg aaaagtcggg cggatctagc tcctgccatg
    27121 gaccttttaa gagaaggaac ctgtgtttta gaagcatgga tcccgtttga aaaagagatt
    27181 tcgatcatgg tggcaggaaa tggtcaagga gatttcacga cttttccggt cgtggaaaac
    27241 atccaccaca acaatattct tcatgaaacg attgctccag ccgcgattga tcaggatgtg
    27301 atcgaggagg ctgagcgtat tgcccgtgtg attgcagaag cagtgagcct ttcaggtgtt
    27361 ttaggggtag aaatgttttt aacaaaaaca ggcgggctat atgtgaatga attagctcct
    27421 cgtcctcaca attctggaca ttattctatt gaagcttgtt ctatgagtca gttcgatgcc
    27481 catattcgag gtatttgtgg ttggccatta ggagaaaaag tacgtttgat aagcaatgca
    27541 gtaatggtca atgttttggg agaacaactg ctggaaagct atcgcttgat tgagaataag
    27601 ccagactggc aattccattt ctatggaaaa aaacaagcaa aaaaaggtcg caagatggga
    27661 catatcacta tcttgacaga agacatccag caaacgctgg aagaaatcaa acaaacgaat
    27721 atttgggatt agaggagaaa aaacatgtta gaacgttata cacgccctga aatggggaat
    27781 atctggacgg aagagaacaa ataccgtgcg tggctcgaag tcgagatttt agcagatgaa
    27841 gcttgggcag aacttggtga aatcccgcaa gaagatgtga aaaaaattcg tgagcatgca
    27901 tcatttgata tcgatcggat cttggaaatc gaaaaattaa cacgccatga tgtcgttgcg
    27961 tttacacgag cggtttctga aacattggga gaagaaagaa aatgggtcca ttatggtcta
    28021 acaagtacgg atgtcgtaga tactgcttat ggctatctca tcaagcaagc aaatgatatt
    28081 ttacgggaag acttgaaacg ttttacggaa atcattggcg aaaaagcaaa agaacacaaa
    28141 tatacagtga tgatgggtcg gacacacggt gtccatgctg aaccaactac ttttggattg
    28201 aaacttgcca catggtattc agaaatgaaa cggaacatcg aacgatttga acatgcggca
    28261 aaaggagtag aagcaggaaa aatctcagga gcagtgggaa cgttcgcaaa tatcccgcca
    28321 tttattgaag agtatgtatg taaacaattg gggatccgtc cacaagaaat ttctactcaa
    28381 gttctccctc gggatctaca tgctgaatat ttctcagcga tggcgcttat tgctacaagt
    28441 attgaacgtt ttgcaacaga gatacgcgga ttgcaaaaat ccgaaacacg tgaagtagaa
    28501 gaattttttg cgaaaggtca aaaaggttct tctgctatgc cgcacaaaag gaatccgatc
    28561 ggttcagaaa atatggctgg tctagccaga gtggtgcgcg gacacatggt cactgcctat
    28621 gaaaatgtca cattatggca tgaaagagat atttctcatt cttcagcaga acgaatcatc
    28681 ttgcctgata ccacgatttt attgaattac atgttaaatc gttttggcaa tatcgttaaa
    28741 aatctaacgg tctttcctga aaacatgaag cggaatatgt ctgcaacatt tggtttgatt
    28801 tatagccagc gcgttatgct gaaattgatc gataagggaa tgacaagaga agaagcttat
    28861 gatcttgttc agccaaaaac tgcgtacgct tgggacaacc aagtgcagtt ccgcccgctg
    28921 ttagaagaag atccaaccat cacatcggta ttatctaaag aagaaataga cgatgccttt
    28981 gattatcatc atcatttgaa aaacgttgac gtgatattca aacgagtagg tttggaataa
    29041 agaagagtga gttgcaaaat tcaattaaaa tagaatagca aaaataacca aaaaagaagc
    29101 agatccgtta gcggttctgc ttcttttagt ttattatatt ttcttatcaa taataggaac
    29161 ctgattttaa aagcggctac ctctttaccg tgcctcttta catacaaaaa atagaggaat
    29221 taagcgtaaa tggcttggct attataacta aaatgctggt tatggagatg ttgcagcaag
    29281 ttttcgagaa aagcgtattc catctcttgt ccattgaata agatctggat acgataatga
    29341 tttttgaaac gcttctgttc atgaatctga ctgattgaaa gttcgccttt gtgttctgtc
    29401 tgataaagaa aagtcctttt acggcattca gcacagcgct tggttgctcg gcggattttt
    29461 cggaatgtat aaaaactagt atctttattg cataaataaa cgtctttgat cgacagcaaa
    29521 tcattatata gacaggaacg ttcttcagct gttaattctg ataaaaaaag ctgaccttgt
    29581 tttattgcat cttccatcgg caagtgcctc ctcgaaccca ttatccaaat ttttagttaa
    29641 ttctaatata ccatcattaa gtataaatgt aaacgcttgt tgatggtata agattttcgt
    29701 ttctgataaa acacgaacat taaaagaatc taatgcctta aatgtacata tttttgttga
    29761 tttataaaaa gatgattgct aaaatttaaa gtgaaaaaac gaacaaaaaa cgatgtattt
    29821 gcttttaatg taaataaaaa ggagagacgt acatggagaa gaaagaactt ttgtatgaag
    29881 gaaaagccaa aaaaatatat cttacttctg atgagaactt gatttggata gaatatttag
    29941 atcaagcaac tgcgttgaat ggcgcgagga aagaccagat aagtggaaaa ggagaattga
    30001 ataatcaaat cacaagtgca attttccgtt atctgactca aagaggaata aaaaatcatt
    30061 ttattgaaca attgtctaaa accgaacaat tagcaaaaaa agtacaaatt atccctctgg
    30121 aagtggttat ccgaaatatt gcagcaggaa gtttctcaaa acgtctaggt gttcccgaag
    30181 gtaaagaatt catcaaacca atcattgaat tctactataa ggatgaccag ttggatgatc
    30241 catttatcaa tgaagagcat atctatttct tacaacttgc aaagccggaa gaaatcagtg
    30301 agctgaaagc ccaagcgttg gctattaatg aaattttgac cgaattattt gctaaggtcg
    30361 gtttggtttt agtagatttc aagctagaat ttggccggtc gaatggacaa attttgcttg
    30421 cggatgaagt aacaccggat acttgccgat tatgggatga acgtacgaat gaacatatgg
    30481 acaaagacgt ttaccgcaga gatctaggtg atttgatccc agtctatcaa gaagtatata
    30541 accgtctatc ggaactaact gaagaaaagg agcgagtatg atgtatttcg taaaagtata
    30601 tgtaacctat aaagattcag tgttagatcc tcaaggggag gcagtaaaaa aagcagtcca
    30661 tcgtatggga tatgaaacaa tcgaagatat ccgaatagga aaatactttg agatacaact
    30721 tgctgatgca gaagaaccaa tcgatacagt gattgaaacg atttgtgaca agctgcttgc
    30781 aaatgtaaat atggaaacat accgatatga aatcaaaaaa atggaggaag tataacatga
    30841 aatttgcggt catcgttttt ccaggttcaa attgtgacgc agatatgtta tgggcagttc
    30901 gcgagatcat gggcgctgat gccgaatttg tacggcatga tgagtcctct ttagaaggat
    30961 ttgatggggt tttactgccg ggaggattct cctatggtga ctatttgaga tgcggtgcga
    31021 ttgctcggtt ttctacaatc atggaagaag tgattcgttt tgctgaagaa gggaaaccag
    31081 tattcggtac atgcaatgga ttccagatat tgacagaagc aggacttttg ccaggtgtac
    31141 tgagacgaaa tgagtctttg cattttattt gcaaaactgt accattgaaa gtcaacccca
    31201 agacggcctt tacttcagag tatgaagccg acgaaataat ctatcttcca gttgctcatg
    31261 gggaaggaaa ttatttctgt gacgaagaaa cacttcaaga actaaaaaaa aataatcaga
    31321 ttgcctttac ttataatgac aacatcaacg gcagcgtgga aaacattgca gggattacga
    31381 acaaagcagg gaatgtgtta ggaatgatgc ctcatccaga acgtgcagta gaagacttgc
    31441 taggatcaga agatgggaaa cggttctttg cttccatttt aaaaaacttc ggaaaggtga
    31501 ccgtatagat gaaaacattg aaagaaccga cagctgaaga gatcaagaaa aaaagaatct
    31561 atgccgaatg gggattgact gatgaagaat atcgaatgat cgaagaagat atcttaggaa
    31621 gaatgcctaa ttatacggaa actggactgt tttctgttat gtggagcgaa cattgttcct
    31681 ataaaaattc taaaccggtt ctacgcaaat ttcctactag tggacctcat gtgttgcaag
    31741 gccctggaga aggcgcaggc atcgtcgaca tcggtgatgg actagcagtt gtcttcaaag
    31801 cagaaagtca caatcatcct tcagccgtgg agccttatga aggagctgcg acaggagttg
    31861 gcggaatcat cagagatatt ttcagcatgg gggctcgtcc aattgcgttg ctagattcat
    31921 tgaggttcgg cgaattagat aatccaagaa cgaaatattt actagaagaa gtagttgcag
    31981 gtatcggcgg atacggcaat tgtatcggca taccgactgt aggcggcgaa attgcatttg
    32041 atccttgtta tgaaggaaac ccattagtca atgcaatgtg cgtaggtttg attgaacaca
    32101 aacatatcca gaaagggcaa gccgctggcg tcggtaattc gatcatgtat gtaggtgcaa
    32161 aaacaggtcg cgacggtatc cacggagcaa cttttgcctc agaagaattt aatcaagaag
    32221 aagaacagca acgttctgct gtacaagtag gcgatccttt tatggaaaag ctgctgcttg
    32281 aagcgtgttt ggaattaatc ttgcatcatt cggattctct aataggtata caggatatgg
    32341 gagcagcagg acttgtttca tctagtgcgg aaatggcttc aaaagctggc tcaggactta
    32401 tattgacatt agatgaagtc ccacaaagag aaactgggat gacaccttat gaaatgatgt
    32461 tatcggaatc acaagaacgg atgttgattt gtgttaaatc aggagaggaa gaaaaaatcc
    32521 aagaactatt tcagaagtat gatctagatg cagtcactat cggtaaagta acagacgacg
    32581 ggcaatatcg gctatatcat caaggagaag aagtagcaaa tctgccagtc gatgcattag
    32641 ctgaagatgc accagtttat cataaagaaa tgaaagaacc agctcgaatc gtagaatttc
    32701 agcagttagc tccttatcag ccagttatcg aagaacctgg acaagtattg cttgatctgc
    32761 tgcagcaacc gacgattgct tcaaaacgtt caatctacga aacttatgat tcacaagtac
    32821 aaaccaatac agttgtccgt ccaggaagcg atgctgctgt gatgagggtt agagggacga
    32881 ataaagcgtt agctatgacc acagactgca acgcgcgcta tctgtatttg gatccaaaaa
    32941 tcggaggaca aatagcagtg gcagaagctg ctagaaatat tattgctagc ggtggtaagc
    33001 ccttggcgat caccgactgc ctgaactatg gttctcctga taaaccagaa gttttttggg
    33061 aattatcgac ttcagctgac ggaattgctg ctgcgtgtga aacgttagga actcctgtta
    33121 tttcaggaaa tgtctcttta tataatgaaa cggatggtca agcaatctat ccaacaccaa
    33181 tgattggtat ggtcggattg attgaggatc ataaacatat cactactcag gaattcaaaa
    33241 aatcagggga tctaatctat atattaggta agacgtttgc agattttgat ggcagtgaat
    33301 tacaaaagat gcagcttgga agaatcgaag gtgtgatccg aaactttgat ctatccattg
    33361 aaaagagaaa ccaagagtta gtattgacag ccatccaaaa cggattgatt gaaagtgctc
    33421 atgactgttc agaaggtggc ttggcaattg cactggcaga atcagctttc aaacatcagc
    33481 tagggatatc tgtacagttt gaattgtcct ctgctcaatt atttgcagaa actcaatcaa
    33541 gatttgtgtt gacagtcgca ccggaaaata agacacgatt cgaagaaatg atgggagatg
    33601 ctgctgtttt agcaggtaag gtgacggatg aagcgataat cgaaatttca gcaactgacg
    33661 gtcagatcaa aatagaaact gcagtagcaa gaaagtgctg ggaggatgca atcgtatgtc
    33721 ttatgaagtg aagagtttaa atgaagaatg cgggatcttc ggtgtttggg gtcatccaga
    33781 cgccgctagt gtggcatact tcgggctcca tagtcttcag catcgaggac aagaaggtgc
    33841 aggtatcgtg tccaatgatc gaggaaagct taaggggcat cgagatttgg ggctgctttc
    33901 tgaggtattc aaagatcaaa gaaaattgca gcagttaaca ggagaagctg caatcggaca
    33961 tgtacgttat gcaacagcag gcaatggaag tgtggacaat attcagcctt ttctattcaa
    34021 atttcatgat cagtcgatcg gcttagcgca taatggtaac ttgatcaatg ctcgttccct
    34081 tagaaaagag ttagaggaaa atggggcgat cttccattct aactccgata cggagatatt
    34141 gatgcatttg atccgacgaa gtgaagaaac agatttttta aataaattaa aaagtgcttt
    34201 gaatgaagtc aaaggtggct ttgcctattt attaatgaca gaaacggcaa tgattgccgc
    34261 tttggaccca aatggttttc gtccattagc aatcggacaa atgaaaaacg gagcatacgt
    34321 gattgcctcg gaaacttgtg cattagaagt tattggggca cgttttataa gagatgtcca
    34381 gcctggtgag atagtgatca tcaatgacga aggaatccag attgattgct ttacgaagga
    34441 cacgcagctt tctatttgtt cgatggaata tatctatttt gccagaccag attcaaacat
    34501 cgcagggatc aatgtgcata ctgcacggaa aaatatggga cggaatttag cgaaagaatc
    34561 gccagtaaaa gcagatatgg tcgttggcgt acctaattct tctttgtcag ccgcaagcgg
    34621 ttatgcggaa gctagtggaa tcccttatga aatcggatta gtaaaaaatc aatacattgc
    34681 ccgaacattt atccagccaa cgcctgagct aagagagcaa ggtgttcgaa tgaaattatc
    34741 tgctgtacga ggagtagtgg aaggtaaaaa ggtgattatg gtcgatgatt caattgttcg
    34801 gggtacgaca agcagacgga tcgtccatct tttgaaagaa gcaggggcaa aagaagtcca
    34861 tgtgaggatt gcttctcctc ctttgaaata tccatgtttt tacgggattg atattcaaac
    34921 gcgtaaagaa ctcattgcgg caaatcacac gattgatgaa atcaaagaat gtatcggtgc
    34981 agattcttta ggttttttaa gtgaagaggg tctgatcgaa tcgatcggtc tggaaaaaga
    35041 tgctccgtat tcaggattgt gtatggctta tttcaacgga gattatccga cgccgttata
    35101 tgactatgaa gaaaaatatc aggaatcact aaaagaaaaa gtatcgttct tttaaaagca
    35161 gaccaagaag ggagtatatg aattcaatgg caaatgcata cgcaaaagct ggcgtagatg
    35221 tcgaagctgg atatgaagta gtagaacgga tcaagaagca tgtcaaaaaa acggaacgtt
    35281 taggtatgat gggcgctata ggaggattcg gcggttgttt tgatttatcg gtctttcaat
    35341 taaaagagcc agtccttgtc tcggggaccg atggcgtggg gacgaaatta gcagtggcaa
    35401 tcgaagcaga caaacacgat acaatcggga tcgactgcgt agcgatgtgt gtcaatgata
    35461 tcttagcaca aggcgcagag ccgttatatt tcttagacta cttagcaacg gggaaaaatg
    35521 atccgttacg tttggaaaat gtagtaaaag gtgtagcaga tggatgtgtt caaggaaata
    35581 tggcgctgat tggcggagaa acagctgaaa tgcctggtat gtactcaggt aatgattatg
    35641 atctagccgg atttgctgta gggatagctg aaaaatctca attattgaca ggagaaacga
    35701 tcaacccagg agatgtgttg cttggaatag catctagcgg gattcattca aatgggtact
    35761 ctcttgttcg caaaattttc tttgagcagc agcagttttc tttatcatca aaactgccag
    35821 aattgaaaaa aacacttggc gaagaattgc ttgagccaac gaaaatctat gtttcttcct
    35881 tgcttccgct gatcaaagaa aagaaaataa aaggagctgc acatatcact ggtggagggt
    35941 ttgtagaaaa tatcccgcga atgttaccag aatctatggc tgcacaaatc aaacttggct
    36001 catggccgat tcttccaata ttcggctgtt tacaaaaata tggtgaaatt cctgagatgg
    36061 agatgtatga aatcttcaat atgggcatcg gtatggtcct ggcagtcgaa gaatcgaaag
    36121 taaaagaaat agtacacgaa attgagcaag ctggcgaaaa agtttatgaa atcgggaaag
    36181 tgatcgccaa agaaaaacat cgtgtggtgt ttataggaga agaacgatga gaatcgctgt
    36241 ttttgcttct ggaaacggaa gcaactttca agcgctagcc gattatctat cgaaaaaagg
    36301 attggaatct tcgattgact ggttgttttg tgatcagcct gaagcatatg ttttgaaacg
    36361 tgcaactgcg ttgagtgtac cggcagattg tttttcacct aaagaatttg attcaaaaaa
    36421 agagtatgaa gaagcaattt tgcataaatt gaaagaaaaa aagatcgatt tgatcgtatt
    36481 agctggatat atgcgaatca ttggaccggt attgctgaaa aattatgata aaagaatcat
    36541 caatatccat ccgtctcttt tacctgcctt ccctggtttg catggcatac gtgatgcctt
    36601 tgaagctgga gtaaaagaaa cgggtgtcac aattcattat atcgaccaag gtgtagatac
    36661 tggaccaatc atccggcagg aaaaagtgag aattgaacag gaggacacgt tcgattcttt
    36721 agaagaaaaa atccatagag tagagcatcg aatctatcca gaagtgatta gtgaaattat
    36781 tgaaaatgga ggctatctga aatgacaaga gcattgatca gtgtatcaga taaaactggg
    36841 attgtagcat tcgcccaagg tttgattgaa caaggagtag aaatcatttc aacaggagga
    36901 acaaagcatg cgttggataa agctgggatc aaaacgcttt cgatcgaaga agtgacaggg
    36961 tttcctgaaa tgatggatgg ccgtgtcaaa acacttcatc ctaaaatcca tggtggtctt
    37021 ttaggaagac gcgatctccc tagccatatg gaggcaatgg aaacggaaaa tatccaacca
    37081 atcgactttg tttgtgtcaa tttatatcct ttcaaagaaa cgattatgaa accagatgtc
    37141 gaaatagcag aagctattga aaacattgat atcggtggtc ctagtatgct ccgtagtgca
    37201 gcaaaaaacc atgaatttgt cacctctgtt gttgatccat ctgactatga agaagtactt
    37261 tcagaattac gtcagtatgg gcatacttca ctttctttca gacaaaaact agcggcaaaa
    37321 gtctttcgtc atactgcggc atatgatgca ctgatcgctc aatacttaac ggattgggtg
    37381 gaagaaaaag aaccggaaaa attgacactt acgtatgaac gaaaacaaac gcttcgttac
    37441 ggtgaaaata gccatcaagc cgctgccttt tatcagtccg ctcttctgga aaaatattcg
    37501 atcgcagcag caaaacaact ccatgggaaa gaactttctt ataataatat ccgagatgca
    37561 gatgctgcgc tacggatcat gaaggaatat gacgagccaa cggtagtcgc cttgaaacac
    37621 atgaatcctt gtggtattgg gacagcatct acgattttag aagcgtggga agcagcgtat
    37681 gaagcagatc ctgtttcaat ctttggtggg atcatcgttt tgaatcgaga agtagatctg
    37741 ccaacagcag aacagatgca taagctgttt ctagaaatca tcattgcccc ttcttataca
    37801 caagaagctt tggaattact gcaaaaaaag aaaaacatcc gattactgac agtagacttt
    37861 gcgcaggaag aaaatcaaaa tcgagaagaa acggtctcag tcttaggtgg acttcttgta
    37921 caagatcaag atctggcaat agaaaatgaa gaagagtgga aagtcgtgac aaaacgtaag
    37981 ccgactccag cagaagcaaa agcattagtg tttgcttgga gagctgtcaa acatgtgaaa
    38041 tctaatgcga tcgtactggc aaacgaacgt cagacagtag gaatcggagc cgggcagatg
    38101 aatcgagtcg gttcagtaaa aattgcgatt gaacaagcgc aagagaaaat ggaaggtgca
    38161 gttttagcta gcgatgctta ttttccaatg gatgattcgg ttgaatatgc agccaaacat
    38221 ggtataaaag caatcattca gccaggagga agcatcaaag accaagattc tattgatatg
    38281 gcgaataaat acgatctagc aatggtcttt actgatgttc ggcattttag acattagaga
    38341 ggagtcggat aaatgaacat tttggtgatc ggtgcaggtg gtcgtgaaca tgcaataagt
    38401 aaaaaacttt tagccagtcc gaaggtggat acggtgtatt gtgcgccagg aaatccgggg
    38461 atgacaaaag atggtatcaa ttgtgtcgct atcagtgagc gagatcattc agcgttaatc
    38521 cattttgtta agaaagaggc tattgattgg agtatcgttg gtcctgaagt tcctttgtta
    38581 aatgggatcg tcgatgattt tatggcggaa ggattgaaaa tcttcggtcc gacaaaagca
    38641 gcagctctga tcgaaggttc gaaaagtttt gctaaacaat taatggatac ttatcaaatc
    38701 ccgacagcta aatccagaag ttttgaatgt tacgaaaaag cagcagcgta tatcaaagaa
    38761 aatggtgcgc caatcgtagt gaaagcggac ggcttagcag ctgggaaagg tgtagtcgtg
    38821 gctaccacag tgaaggaagc gctggatgcg gcagaacaaa tgttgaacca gcatcgattt
    38881 ggggatagta gcaaaaaaat cgtcgtagaa gagtttttgg caggagaaga attctccctt
    38941 ttggcgtttg tccgtaatac caaggtgtat ccgatggtca tttcgcaaga tcataagcgg
    39001 gcatttgata acgataaagg gccaaataca ggcggaatgg gtgcttattc accagtaccg
    39061 caaatttcaa aaaagatgat tgaaaaagct gtggaagaag ttttaaaacc agcggctttg
    39121 ggaatgaaag aactaggacg ctctttcacg ggtgttttat atgcgggttt gattgctacg
    39181 aaggaaggac caaaagtgat cgaattcaat gcgcgtttcg gtgatcctga aacgcaagtc
    39241 gttctttctc ggttgaagag tgactttgcg caagtaatcg atgatttatt agagaatcgt
    39301 acagtagagt taaagtggca gcaagaagga tacaatgtag gcgttgtggt tgcagctgcc
    39361 ggttaccccg aagaatatga gacaggaatg gttctaccag attttctgga agaagagctt
    39421 tctgtttact atgctggagt ttcctcaaat aacggcaaac tagttggagc aggaggacgt
    39481 ctttatttag tagaagcata cggtgagacg atcaaagaag cacagaaaaa aatttacacc
    39541 gcgttaatgg ataaaacgac agatggaaca ttttatcgta cagatattgg agaaaaagct
    39601 gccaatagcg aactgatcgt gaagtaaatc agctgcttcc atttataaaa aatacaacaa
    39661 acataatcgg aagcgccgat tttagtgaag ccttttcata aagagtgtgg tacacttttg
    39721 ttgtgtatat aaataaggag gagttcagat gaaaaaaatt caattaggta cttctgatca
    39781 gaaagcagct gcagtgatct taggctgtat gcgtatcaac aatgcggaag atccagtaaa
    39841 agtgattgag acagcttatg aaaacggcat tgattttttt gaccatgcag atatttatgg
    39901 aaacggtgaa tgtgaagaaa tctttgcaga tgctctagca aaaacatcaa tcaaacgaga
    39961 agatcttttt attcaaacaa aatgtggaat cgttccaggt gtgatgtttg atttttctaa
    40021 aaaacatatc attgaatctg tagaaaaaag tttgacccgt ctgaaaatgg attatgtgga
    40081 tgctttactt cttcatcgtc cagatacatt ggtagaacca gaggaagtag ctgaagcgtt
    40141 tgatgaattg gaacggtcag gaaaagttcg ttattttggc gtcagcaatc aaaaaccaat
    40201 gcaaatcgaa ttgctgaaaa aatatgttcg tcaaccatta gtagctaatc agctgcaatt
    40261 tggcttgaaa catactggaa tgatcgatca agggattcat gtcaatatga cagatgaagc
    40321 aagtatcgat catgacggta gtgtacttga ttattcccga ttgcatgata tgacgatcca
    40381 agcatggtca ccataccagt atggattttt tgaaggtgta tttatcggga acgaaaaatt
    40441 ccctgaatta aaccaaacat tagaagaact agcagctaaa tatcaaacga caccaactgg
    40501 cttggcttct gcttggattt tacgccatcc agccaatatg caagtgatcg ccggtacgat
    40561 gaacgtaggg cgaatcaagg aaatcgctca agcttcagaa attaatatgt ctcgggaaga
    40621 ttggtacgaa ttgtaccgtg ctgctggaaa cattttacct taaagattgt cataagcagg
    40681 tcaaaagggc tataaattaa aaagacagaa taacttgaaa gctgttttca gcttcgagat
    40741 attctgtctt tttttattgc aaatggaaag agtatcattg tacggatttc gtttattagt
    40801 tatttttgtt tttaaggaaa agctgtgtcc gatattcttt tggagaaatc ccttgttttt
    40861 tcttaaataa ataactgaaa tagttagggt cattataacc aatttcaaaa gcaatatctg
    40921 aaatacgata gtctgattgc tgcaataatt tttttgctaa ttcgattctt tgttctgtca
    40981 gataatcgat gaatgtttgg ttaaacgatt gagaaaaaat cgtactgaaa tgagagggac
    41041 ttaatgcaac ttgttgagcg acccaagtta aggacatatc tggatcggta aaattttctt
    41101 tgataaaatc caatgcatgg ttcaaaattg gctgatattt gttccttgaa ggctgtgagg
    41161 gcagagcagt taagaaagaa atgaacatat aaactgtcga acggtaaagc gagtcgtcgg
    41221 cgctgattgc taagcgttga ctggtggatg tgcaatgaac catttcttgc gggattttct
    41281 tttctttcag gcagacatgc tggagtagct tagtcaagac aaataaacga tataatcgtg
    41341 ttcgagttgg tgtgccttgt ggtttcgtca agtgttcgat gtacttagat acttcgtttt
    41401 tcgataatga agacaaatca agtgagaggt caaagtaaaa gacagaatca actggaagtt
    41461 tcgcttcttg aatcaaatcc tcatagctga taatttgttc tttaagggaa ctggcggttt
    41521 caagtatcat ctgcgtgatc tcaaaagatg caggtaattc gctgattctt tctgcgacag
    41581 gaccaatggc gacagaaaaa ttattttttt gtcccttttc taattcatgg accaacgttt
    41641 gggctacctg ataactggtc tcgatcacat tttcttcaag agaatcaact attaaaaatt
    41701 tgatgaatcg agaggaaaca gcagagaaga tcacttgttg attctcccta aataaaaact
    41761 tcaaataatc actgagatga gcgtaatctt caaagtctgt actgacttgg tttgctgcta
    41821 ataatacggt catttttttc cctgctaaag agcgattgaa agattcagca agtttcaatg
    41881 tttcactgat cgtaagtgag cctttgaaca aaccgttcaa gaaatgattt tttttcaatt
    41941 cgaaaacaag atcagaagaa gtgtgcgttt ctttttgctg caaatctaaa gcagttactg
    42001 ctttttctat tgcttgtttc aattcgctac ttttaatcgg tttaagcaga taatcatctg
    42061 cctgtacctg gattgctcct ttcatataat cgaaatcgtc gaatccacta ataaatatga
    42121 gacgagtcca aggaagtagt ttccttgctt cttttgaaaa agtcaacccg tccatgaaag
    42181 gcatttggat atcagtcaga atgatgtcgg gctgtaagtc gataattgaa gacagtgcca
    42241 tctcaccatc tccagcttcg ccgacataag tgattggata ataagaacca aggacagtta
    42301 tttgttcccg caagttattg cgaatgaggt attcgtcttc gatgataaaa actttataca
    42361 tgtttgtttc ctccaattgt tttgggagat agagggatca tcagtgtgac agttgttcct
    42421 tctcggtatt tgctgtcaat cgtgataggt ttttgatcat ttccgtaata aagaaggaga
    42481 cgcttgttta cgttgtaaag tccgtagccg atactgaaat ctgaatcgat ccctttggct
    42541 aactctttac gtatttctgc cagtttttct gcagggatgc caatcccgtt gtctataact
    42601 ttgaattgga tcatctcatc aattttgcat gtggtaatac gaataaatcc tttgtgacgg
    42661 acgtacttcg ttccatggta tacagcattt tcaatcaatg gctgcaagat catttttaaa
    42721 atagaaacag gaaaaagtgt ttctggtata tcgatcttat agtcaagcat atggccgtat
    42781 cttattttta agatgactag ataatcattg atatggctaa tttctttttc aagaggaatc
    42841 cagtcttgtc ctttgcttaa agacgtacga aaaaagccgg aaagtgcatg ggtcatagtc
    42901 agagcttctt caaagcagtc ttgttcgatc aaagaaataa tggcatccaa actgttataa
    42961 ataaaatggg gagtaatctg agcttgcaat acacgaactt cactttgtgc taaatggtat
    43021 tgtttcaaag cgttctcccg taaagactgg tttagatctt ccgccatttt attcatgcta
    43081 gcggtaagtg tttgtaattc cagtgtttcc ggcagggtta aacgaaagcc aaggttccct
    43141 gaagaaagtt gttctgacat ctcgatcagc tgattgatcg gttcttggac ctttctctct
    43201 aaaaagcgac gattcttttt cgcatagaaa aaaatggtac taagaactcc aagttccact
    43261 attgttaata gaatgagcga gcggacaagt gtcgtatttt ttttgcttgc taagtcgatc
    43321 tccacacgaa caaaatcttg taagatatct gacagcagtt gagtgacaga atctacctga
    43381 ttgatgatct cttcattggt atccataaaa tcttttgcca agatattatt gatcagctcg
    43441 ttttgataat cttccagtgt atcgatcgta cgtagcgata catctaacgt actaatttct
    43501 ttaagtgtat ttgtattatt tttgctctct cgaatatcat tcctaagttc ttctaggata
    43561 ttttcctcat tgaactcatc tacggttact tggccagaaa tcaagtccca catactttcc
    43621 aaaacttttt gatcaacctt caaagcaatt gcatctgctt tttggatatt tgcgatcgtc
    43681 tgttgataaa tcaaaagctg ttgcgaatat aacagtatgc tcagtagcaa agggataaca
    43741 gcaaatagaa gcatggcttt gtttgcttgg gcaaaaatct gttttatgct taaatttttt
    43801 gtcatgatac ttctccagtc aataatttct ggttgggatg ttagctatat tctgctggga
    43861 aaagacagtc tcggaaataa aaatatcttt aggtaatgtt tttcctgttt gaatatttcc
    43921 aaaataacga gtaacggtat tggctacata ccagccgaca tcaggtttac actcgactac
    43981 acaattgacg attcctgagc gtagcttctc gatcatatct tcttgtccat caatagttac
    44041 aatgatcaaa tcttctccag gtttgattga tgtttctttt atcgcagcta aagcaccgtg
    44101 agtcatttca tcactatggc tgtataatac atcgatttcg gataaatcat ttgtttctaa
    44161 atatcgtttc atctggtctt ttcctttcat ccgaataaaa tcaccgacaa ttcggtgaac
    44221 aatagtcatc ggaggtttat ggtcgatggt tccatctttt tttagggttg gtttacgatt
    44281 gattgattcc aaaaatcctt cggagcgtag cttggtcggt gtggagttct ctgaaccagc
    44341 aagttctagg attctgattt cctgtttctt tgaatttgtg aagtgattgc tgacaaataa
    44401 acccgcccga tttccttctg ctttgaaact ggggccaatg tgggtgacga aaagctcggg
    44461 atcattcgtt tgaatatgtc tgtcgacaag tatgacaggg atccctgctc gctttgcttc
    44521 ttttaataca gagtcccacc cggattcttg caaaggagta aaaatgatca tatccacttt
    44581 ataggcaatg aacgtccgta tatcttggat ttgccgttcc tgattcatgt agccatttcg
    44641 gtagagaact tggtaatctt ctttttcaag cgcagtgata attgattcag tatgcttttt
    44701 ccgccagcta ctctccgttc ctgattgcga aaaaccaacg actagtgggt caggtgcttc
    44761 ctttgtatta ggtgtacacc ccatcatttg gtaagcgatt acaacaaggg ctaaaaaaaa
    44821 taaaacacgt ttcactttaa atcactccat ctggatttgt tagtactatc ttatcagata
    44881 aaagggaaaa tcaaagtcaa aaggaatcaa tcgaaaaaaa tccaagaaac ttaaaaaaat
    44941 ccaagacaaa aaaagtacac ctaaaaaatt taaagtaaat cccataaaaa aatcaatgtt
    45001 aacgctttct ttattagcta tactcggtgt atcaattgaa atgggggata aaaaatgaaa
    45061 aacaaattta gaaagattgt tgggactatc gctattttga ccgcaagcat tctattaagt
    45121 gcgtgtggca attcaggatc tgcagatgat agtacggggt atgtaggaat cgctatgccg
    45181 accaaatcag cagaaagatg gatcgctgat gggaacaaca tggtctcgga attagaaaaa
    45241 ctgggttata aaacagatct tcaatatgga gaagataaag tagaaaatca agtagcacaa
    45301 atagaaaata tgattacaaa aggggtagat acacttgtga ttgcttctat cgatggttcg
    45361 gctttaactg atgtgttagc taaagcaaaa gaagcagata tcaaggtgat cgcctatgat
    45421 cgtttactga tgaattcaga aaatgtcgat tattacgcga cttttgataa ttttggagta
    45481 ggtgtatcgc aagcagctta tattgaagag cacttaggat tgaaagaagg aaaaggacca
    45541 ttcacgattg aattatttgg tggctcgcct gacgacaaca acgcactaat caattacaac
    45601 ggagtgatgt ctgttttaca accatatatg gacaacggtc agttggtcgt gccatctgga
    45661 cagacgagtt tcagtcaaat cgctacgctt cgctgggatg gttctacggc gcaagcaagg
    45721 atggataatc tgttaagcgc aaattatact gaccaaacat tggatgctgt tttgtcgcca
    45781 tatgatccaa taagtttagg aatcatttcg tcattaaaag gcgtggggta tgggagtgaa
    45841 agtaaacctt tacctgtgat tactggacaa gatgcgacag ttgctggcgt taaatcgatc
    45901 attgccgggg aacaaacaca aacgatcttt aaagacacac gtatcttagc aaaaaatacg
    45961 attgaaatga tcaaagcaat cagtgatgga gaagaggttc cggtcaatga tactgaaact
    46021 tatgataatg gggtgaaaac tgtccctacg tatttagcaa atacagtatc tgtcgataaa
    46081 gataattatc aagcagagct gatcgacacc gattattata aagaatccga tctgaaaaat
    46141 taagaggaac caaacaagga actaaagaga tctaagtcag agagaggata agaattatgg
    46201 cggattgtat tttaaaaatg gcagatatca ccaaaaaatt ttctggaatc cgagcgttag
    46261 attgcgtgaa tctgatgatt gaacgtgggg aaatccatgc attgtgcgga gaaaacggag
    46321 cgggaaaatc aacattgatg aatgtcttga gcggcttgta tccatatggc tcctatgacg
    46381 gggaaatctt ttatgatgga aagctgtgtc aatttaaaga tttgaaagat agtgaagaaa
    46441 aaggcatcgt catcattcat caagaattag cactaagtcc ttatctttca ataaaagaga
    46501 atatttttct cggaaatgaa caagcagaaa gaggcgtgat tgattgggaa ctgacagaaa
    46561 agaaaacata tgaactgctg aataaagtcg gtttgtctat cgacccgaat cttctagtat
    46621 cccagattgg cgtagggcaa cagcaactag ttgaaatagc taaagcatta tcaaaatccg
    46681 tccgtctgtt gattttggat gagcctaccg ctgcattgaa tgaagaagaa agcgcgaatt
    46741 tgctgagatt gatccgagag ttgaaagagc aaggtgtaac ctccatcatt atttcacata
    46801 agctgaatga aatcgtggat atcgcagatc gaatcaccat tttacgggat ggacagacga
    46861 ttgagacact agaaaatgaa atgatcagcg aagagcatat tattcgcgga atggtgggca
    46921 gagatttagt tgatcgttat cctgaaagaa aaccggatat tggagaagtt tattttaaag
    46981 tagaagactg gacggtctat caccctcttg atcacgagcg aatcataaac gaccacatca
    47041 acttgcattt gaaaaaagga gagattgtag gaatagctgg actgatggga gcgggcagaa
    47101 ctgaattcgc tatgagtcta tttggacatt cttatggaag caagatatcc ggacgtatat
    47161 taaaggaagg ggcagagata tcagttaaga ccgttccttc agccattgca aacggactcg
    47221 cttatgtctc ggaggaccga aaatctttag gactgaatct gctgatggat attcgggaaa
    47281 acgtgacgct gagcagtttg gataaaataa gtcaaaaagg agtattagat aaagaaaaag
    47341 aagtaactga ggcagaaaat ttccggaaaa agatgcgtat aaaaacaaac tccatccatc
    47401 aaattgtcag tagtcttagt ggtgggaatc aacaaaaagt cgttttatcc aagtggctga
    47461 tgacacaacc cgatattctt tttttggatg agccaacccg tgggatagat gttggggcaa
    47521 aatatgagat ctacacgatt atcgaagaaa tggcagcaaa tggcaaagct gtctgtctta
    47581 tctcttctga acttcctgaa atattaggga tgtgtgatcg aatctatacg atgaacgaag
    47641 gacgtattac aggagaagtc tcgcgagaag atgcagatca ggaagtcttg atgaaattaa
    47701 tgacgaaaga agaggtgggt taagttggaa caggcaaaaa atcttacgga taaaaaagag
    47761 aaaaaactaa atttgaaaag tagagcagct gatctttttg gtcgctacag catggtgatc
    47821 attttagcgg gactacttat tgcgtttcaa ttgatgacgg atggtatttt ctggcgtcca
    47881 ttgaatataa ctaaccttgt tctgcaaaat agccatatat tagtactagc tgcagggatg
    47941 ttattagttg tgctcttagg tcatgtggat ttgtcggtag ggtcagtgat ggcgtttgtt
    48001 ggtgcaattg cgggcatgat gatggtcaat cgccaaatga acccgtggat gacagttatc
    48061 gcatgtcttg cagtaggcgg gctgatcggc gcatggcaag gtttttgggt agcttatgca
    48121 gggataccgg cgtttatcgt gactttagca ggattgctga tgtttcgtgg actgacacaa
    48181 gttgttctag gaggccaatc tctagcacct tttccagctg ttttccaaaa aatgtctacg
    48241 ggttatttac cagacttaag cgaaggaagc agccttcact tcttgacatt gattttagga
    48301 atgatcatca cgatcgtgtt agttgttggt caatggcgaa caagaaatcg tcggaaaaat
    48361 catttatttg aagtggaatc tccaacagct ttcttggtaa aggcggtatt tacagcagtc
    48421 gttgtactag gaataactta tatctttgcc tcttatcaag ggtatccgat tattttgatc
    48481 attcttggtt tgatcgttgc agcatatggg tttttgacaa ataaaacagt agcaggtcga
    48541 cagatttatg cgacgggcgg aaatcgaaaa gcggctgaac tatcagggat caaaacaaag
    48601 cgaatcacgt tctgggtatt cgtcaacatg ggagtcatgg cagctttagc tggattagtt
    48661 ttagctgctc gattgaatgc ggcaacacct caagcaggta catcgatgga actggatgca
    48721 atggcggcag tatattttgg tggtgcttcg acttccggcg gcattggaac aatcatgggt
    48781 gcgattgtcg gtggcttggt catgggtgta ttgaacaatg gcatgtcgat tttaggcgtc
    48841 ggggtagact ggcagcaggc aatcaaagga ttgatcttgc ttttagccgt tgtgctagac
    48901 ttgtataata agaaaaaacg aacaacgtag gagaacgccg gttattcaga aaaaagactg
    48961 tgacaagaca tttgtcacag tctttttttc gctaaaagcg aacattcatt gtttcgtttg
    49021 tatagactaa aaataatata tgttttttta ccattcgtat aaattctctt tttgctcttg
    49081 atttttttgc ttaattgagt taaattacat agggcgctaa aaaaagcctt gattaaatta
    49141 ataatgttcg tgttttgctg gatgtgaaca tcaggaggat aatgtaatga aagtatttga
    49201 ttacgaagat attcaattaa ttcctaataa atgtatcgta aatagccgtt ctgaatgtga
    49261 tacaacggtc acattaggta aacatacatt caaaatgcca gtagtgcctg ccaacatgca
    49321 gacgattatt gatgaaacaa tcgcagaatt cttagcggaa aatggttatt tctatatcat
    49381 gcatcggttt gatgaagcag ctcgaattcc gtttatcaaa aaaatgaaaa agcgtggatt
    49441 gattacctcg atcagtgttg gcgtcaagaa agaggaatat tcctttatag agaagttggc
    49501 agaagaatcg ctaaatcctg attatatcac tatcgacatc gcgcatggac atgctaactc
    49561 agttattgat atgattcagc atattaaaaa atatttgcct gaaacttttg tgattgccgg
    49621 taatgttggt acaccggaag ccgtccgtga attggaaaat gcgggcgcag atgcaacaaa
    49681 agtcgggatt ggtccaggaa aagtatgtat tacaaaaatc aagacaggct ttgggactgg
    49741 aggctggcaa ctggcagctc ttagatggtg tgcgaaagca gctcgcaagc cgatcatcgc
    49801 tgacggtggg atcagaacac atggcgatat tgcaaaatct gtccgttttg gtgcgacaat
    49861 ggtcatgatt ggttcattgt ttgccggtca cgaggaatct cctggcgaaa caaaagtcga
    49921 aaatggaatc gtgtataaag aatactttgg ttctgcttca gaattccaaa aaggcgaaaa
    49981 acgaaatgtt gaaggaaaga aaatctggat ccaacacaaa ggatctttga aagatacact
    50041 agtggaaatg caacaagacc ttcaatcatc tatttcttat gctggaggaa gagatctgga
    50101 agccattcga aaagttgact atgtcatcgt gaaaaattcg atttttaatg gagacacaat
    50161 ctaaaaaata aataaaatat taggatgttg tgaaaaagtt gtcctgcatt aagtaataag
    50221 aacagccaaa caaaaatagt tctttatatg ttttgtttgg ctggaattat tatcgcagat
    50281 acaagctttt gaacatcatt attcagaata agggactgtg ataagatttt tgtcacagtc
    50341 ccttatgttt tgcaaaaaaa gtttttcttt tttctcgtaa attcggtaaa atcaaaagaa
    50401 aaaatagaca atagaaaaga gaagcttatg tgggaaaaag tagagaaaaa acaaggaccg
    50461 atttatcgtc aaattatgga acagattatg agagaaatcg aaaacggaag gatcaatcct
    50521 ggaagtcaac ttgaatcaga acgaaaacta gggcaacttt ttggtgtgaa tcgttccacc
    50581 gttgtccatg cattggaaga gcttagggag cttggggtac taacgagcaa acgtggcagc
    50641 ggatggtttg ttaatcagac tgaatggggg aaattcgctg ttccaagaat cgattggcgg
    50701 caaatgatct ctcctcgtta tgagcagacg gactggtatg agcaaaaaat aagagaagca
    50761 aagcagatcc aaaaaacatc gtttcttgat ttatacgcta gcgagatgcc agatgagctt
    50821 ttgccgaatt ttgagtttcc ttcttattct cttgaagaaa tcttagcgga agaaaaagaa
    50881 atggatcttt taggctatac tccgttacgt aacaaaatca aacggtattt ggaaagtaaa
    50941 tttgaattgg cattttcccc caaccagctt cttgtgactg gtggcggtca gcaagcaatc
    51001 tttttgattt tacaaacgat tctttcagct ggagagggga tagctgtaga gtcaccttct
    51061 tttttttacc gattacctct tttcaaagct tcaggaacgc gtttatttgg gataccgatg
    51121 gatgaagaag ggatcgactt agaaaaactg aaagaatcga tccataaaaa taaattgaaa
    51181 gcagtgctta tcaacccgaa ttttcaaaac cctacaggaa aagtgatgag tcaaaaacga
    51241 cgagaaaagc tagtagaatt gtgtcgacag tatcggcttc caattattga agacgatgta
    51301 tttacggatc tttcatttct aaaccaagaa gaagtccttc ctatacgaac gattgatccg
    51361 gataatgtgt tatacgtcgg ttcgttatct agggttttag gaaaaacaac gaaaatcggt
    51421 tgggtgatag gacctgagac ttttatctcg caactagctc aagcgcaaga aatgatggag
    51481 ttttcgatga atatccttac acaaatcaca gtggctaatg tatttaatga agcaattgat
    51541 ttgaaaatgc aagatgtaag aaaaaagttg aaagaaaaaa gccgactgat aagcgcttgg
    51601 ggagattcac aatcgttttt caagcttcat tcaattttag gtggttacta tgcctggatt
    51661 acttgggatg ggaaaaaact agaccccgaa ctagcagaaa aagtagtaga tatgggtctt
    51721 ggaattgcac caagtctact ttttgggcaa gatataaatg gaatcagaat caattacagt
    51781 aggctggatg aaaaccaaat accgttgttt tattcaaaga tgaaaattct agaaaagtgg
    51841 ttaacagaat aatttcacaa agtaaataga ttaaagaata taacagcttg gatttaaatt
    51901 tcaagctgtt tctttttttt cggaaaaaga atacgatata aagaaactaa gaaaaaacca
    51961 ttatttgtac gtccgttcac gatgtgtaaa taaaataaat gtttgcaaca aaactgtata
    52021 acaccaacgt ttttaaatag aaacagatgg ttgtaagttt gattagaaaa tagaaaaaat
    52081 gcagatgaaa tataaaacgc tttccttatt gtgattttat tcactaatgc tgttaatatg
    52141 cggattggca taatttcctt tcatcgtgaa aatttgtgct tttagcttgt atttctcata
    52201 aatccatgat aatttattaa tgaagacggt taaatagtga tacagtttta ttcactgttt
    52261 caatcgcaga atgaagagat tttcttatag atgagagagg aatgtgtgac aaatggcaaa
    52321 caaaaaagca ccgatcgact tccaagcact gctagatgaa gtcaatgcag atttcccagt
    52381 atatcaagca cttgatcaag atggaaacgt agttaacaaa gatttagtac ctgatttatc
    52441 cgatgatgaa ctcgttgaat taatgactca tatggtatgg tctcgtgtat tagaccaacg
    52501 ttcaacagcg ctgaaccgcc aaggacgcct aggctttttt gcaccaactg ctggacaaga
    52561 ggcaagtcag ctggctagtg cttacgcatt tgataaagaa gatgttcttc ttccaggtta
    52621 tcgggatgta cctcaactag taaaacatgg tttaccttta tctcaagctt ttttatggtc
    52681 tcgaggacat gctgcaggta attcttatcc ggaagaattg aaagctctac caccacaaat
    52741 tatcatcggt gcacaatatg tacaagcagc cggtgttgca ttaggtttga agaaacgaaa
    52801 caagaaaaat gtcgtcttca cttatactgg tgatggcggt tcatcacaag gtgattttta
    52861 cgaagcaatc aatttcgctg gcgcttacca tgcaaatgcc gtattttata tccaaaacaa
    52921 cgggtatgcg atctctacac ctcgtgaaaa acaaactgca gcaaaaacac ttgcacaaaa
    52981 agctgtcgca gctgggattc ctggtattca agtcgatgga atggatccgc ttgcagtata
    53041 tactgtatct aaaatggcac gggaatgggc gatcagtgga aatggaccag tcctaatcga
    53101 aacactgact tatcgttatg gaccacatac actatctggt gacgatccaa cccgctaccg
    53161 tacaaaagac acagatgacg attggcagaa aaaagatcca ttgatccgct tccgtaaata
    53221 tttgacggaa aaaggacttt ggtctgaaga aaaagaagaa caagtcatcg aagcaactaa
    53281 agaagaaatc aaagcagcta ttgctgaagc cgacaaagta ccaaaacaaa aggtttcaga
    53341 tttcttgaaa aacatgttcg aagtatctcc acaatcaatc aaagaacaaa ttgcaattta
    53401 tgaagcgaag gagtcgaaat aatcatggca caaaaaacaa tgatccaagc aatcacagat
    53461 gccttagcac ttgaactaga aaatgacgag aatgtcgttg tttttggtga agatgttggg
    53521 aaaaacggag gagttttccg ggcaactgaa ggattgcagg aaaagttcgg agaagaccga
    53581 gtattcgata ctcctttagc tgaatctgga atcgcaggat tgagtttcgg tcttgcgtta
    53641 gaaggatttc gtccagttcc tgaaatccaa ttctttggtt ttattttcga agcaatggac
    53701 gaagtagtag ctcaaatggc tcgtacaaga tatcgaatga gcggcactag aaatctaccc
    53761 atcacaattc gttcgccatt tggtggcggt gttcatacac cagaattgca ttcggataat
    53821 ttagaaggtt tgatcgctca gtctcctggt attcgagtag tgattccttc caacccttat
    53881 gatgcaaaag gactattgat ttcttctatt agaagtaacg atccggttgt tttcctagaa
    53941 cacatgaaac tatatcgctc attccgagaa gaagtgccgg atgaagctta cgaagttcct
    54001 ttggataaag cagcagttac tcgtgaggga acagatgtat ctattatcac atacggagca
    54061 atggtgcgtg aagcaatcaa agcagcagac aatcttgcaa aagaaaatat atctgttgaa
    54121 atcattgatc ttcgtacagt tgcgccatta gatgtagaaa cgatcattca atccgtggaa
    54181 aaaactggtc gcgtagttgt tgtccaagaa gcacaacgac aagcgggcgt tgccgcgcaa
    54241 gtagtttctg agatctcaga acgcgctatc ctttcattag aagccccaat cggacgagtt
    54301 tctgcacctg atacagtctt cccatttgga caagcagaaa atgtctggtt gccaaatgca
    54361 aaagatatcg aagacaaagt aaaagaaatc gcggaatttt aagaaaagaa gggacgaaac
    54421 aaagtcatgg cttatcaatt taaattgccg gatatcggtg aaggaatcgc agaaggtgaa
    54481 atcgtcaaat ggttcgtcaa accaggagat acgatcaatg aagatgatac attactagaa
    54541 gttcaaaatg acaaatcagt agaagaaatc ccgtcacctg ttacaggaac ggttaaaaat
    54601 gtgattgttc cagaaggtac cgtagcaaat gttggggatg tattagtaga aatcgatgca
    54661 ccaggacacg aagacaatga aggggacagt ggtgtagccg cagaatccca aactccagct
    54721 aaaccggctg ctgaacccac tgttgataca gaatcagctg gatcgtcaag cgaaggagtc
    54781 ttccaattta agttaccgga tatcggtgaa ggaatcgcag aaggtgaaat cgtcaaatgg
    54841 ttcgtcaaac caggagatac gatcaatgaa gatgatacat tactagaagt tcaaaatgac
    54901 aaatcagtag aagaaattcc gtcacctgtc acagggacag ttaaaaacgt gattgttcca
    54961 gaaggcactg tagcaaatgt tggggatgta ttagtagaaa tcgatgctcc tggacacaat
    55021 agtgcaccgt caactagtgc gccatcagct gaagcaccaa aagaaaaagt ggaaacatca
    55081 ggttcagcaa gtgtcgttga agctgccgat ccaaataaac gtgtattggc tatgccatct
    55141 gttcgtcaat ttgcccgtga aaaagatgta gatatctcac aagtaactgc aactggaaaa
    55201 ggtggcagag taacaaaaga ggacatcgaa aacttcttgg caggtggtcc aagcagtgca
    55261 ccagcgaaat cagaagcgcc agaagcggca gcaccgaaag aagcagctcc tgcagcagaa
    55321 agcaaaccag ctgcaccagc aaaaccattc aagtccaact taggagactt ggaagaacgt
    55381 gtagcaatga cgccaacacg taaagctatc gctaaagcaa tggtcaacag taagcataca
    55441 gctccacacg tgacattgca tgatgaagta gaagtgtcta aactatggga taatcggaaa
    55501 cgatttaaag aagttgctgc tgcaaatggt acgaaactta ctttcttgcc atatgtcgtg
    55561 aaagctttaa cagcgactgt taagaaatac ccagtattga atgcatctat cgacgatgca
    55621 aaccaagaaa tcgtttataa acattactac aatatcggta ttgcaactga tacggatcac
    55681 ggactatatg taccaaatgt gaaagatgca gaccgcaaag gaatgtttgc tattgcagac
    55741 gaaatcaatg agaaagcaaa attagctcat gacggcaaac tatctgcaga agatatgcgc
    55801 aatggaacaa ttacgatcag caatatcgga tctgtaggtg gcggttggtt tacaccagtc
    55861 atcaactatc cagaagtagc tattttaggt gtaggaacga ttgcacaaca accaatcgta
    55921 aatgctgaag gcgaaatcgt tgtcggtcgt gtgatgaaac tgtcattaag tttcgatcac
    55981 cgtatcgtag atggagcaac tgcgcaacaa gcaatgaata atatcaaacg tcttttagct
    56041 gatccagagc tattaatgat ggaaggatga taaacaaatg gtagttggag attttgctgt
    56101 agaattagat acagttgtaa ttggtgcggg acctggtggc tatgtagctg ccatccgcgc
    56161 tgctgaaatg ggacaaaaag tagcaatcat tgaaagagag tatattggcg gtgtttgttt
    56221 gaatgtcggc tgtattcctt caaaagcact gatagctgca ggacaccatt atcaagagtc
    56281 actagattct gaaatgtttg gagtaactag tgaaaacgtc aaactagatt ttgcaaaaac
    56341 acaagaatgg aaagaaaaca aagttgtcca tacgttgact tctggtgttg gtttcctatt
    56401 aaaaaaacat aaggttgaaa caatcgaagg agaagcgttc ttcgtagatg atcacacact
    56461 acgcgtcatt catccagatt cagctcaaac ttattcgttc aataacgcaa tcatcgcaac
    56521 aggttcccgt ccaatcgaga ttccaggatt taaattcgga ggacgtgtgt tagattctac
    56581 tggcggatta gcattgaaag aagtacctaa gaaatttgtg atcatcggtg gcggtgtgat
    56641 tggtgccgaa ttgggcggag cgtacgcaaa cttaggagca gaagtaacga ttcttgaagg
    56701 ctcaccacaa attttaccaa cttacgaaaa agatctagta aaattagtag aagatgattt
    56761 caagaaaaaa ggcgtaacag tcgtaacgaa tgcaatggcg aaagaagcag tcgataacgg
    56821 tgacagcgtg acagtgaaat atgcagtgga cgggaaagaa gaatccgtta ctgctgatta
    56881 tgtgatggtc actgtaggtc gtcgtccgaa tacagatgat atgggcttag aacaagcggg
    56941 tgtcgaagta ggagaacgag gattgatcac ggtggataaa caaggacgta cgaacgtacc
    57001 taacatctat gccatcggtg atatcgttcc aggggcggca ttagcacaca aagccagtta
    57061 tgaagcaaaa atcgctgctg aagcaatctc tggcaaaaaa gtagctgtag attataaagc
    57121 aatgccggca gtagccttca ctgatcctga attggcttct gtaggtatga cgatcaaaga
    57181 agcaaaagat gctggtatcg aagcaaaagc atataaattc ccattttcag ggaacggtcg
    57241 ggcattatcg cttggtaaaa cagaaggatt catccgtcta gttacgacga tcgaagataa
    57301 cgtgcttatt ggcgcgcaaa tcggtggagt cggcgcaagt gatatggtct ctgaattagc
    57361 tttagcaatc gaatcaggaa tgaatgctga agatatcgcc ttgacgattc atcctcatcc
    57421 atctcttggc gaaatcgtca tggatgcttc ggaattagca ttaggtttac caatccatat
    57481 ttgatttgaa taatacaggc gaaacgcaac tgaaaaatca gttgcgtttt gttttttaga
    57541 attatcaata atattattat gtaaacttaa aaatatgaca acttcttggt ttattaccac
    57601 ttcctatgct acactgaaga ggaatatgaa ataggagagt tgaagatgca agaatatcag
    57661 tatccacttg atttagattg gacaacagac gaaatggtca ttgtaatgaa gatgtgggaa
    57721 gatctggaac gtgcgaatga aaaagggata aacaatgaag cttttttgaa gacttatcag
    57781 cagtttaaaa ctgttgtcaa atccattggt gaggaaaaac gattgggacg ggaatttgaa
    57841 aaagcttccg gttattcttt atatcgtaca gtgcagcaag caaaaaaaaa tccaaacaaa
    57901 actttgagaa tgaacgggtg agataaatgg aacagatgat tgatcatatc aagtcctggc
    57961 ttgaagaagc tggtgagaaa atcaaatctt ctcaacaaac attaacaatc tcgcaaaaat
    58021 caaatcgaac ggatctagta acaaatgtcg atcgaaacat ccagaatttt ctaatcgaaa
    58081 aaatccattc ctttgatcca gaggcgaaaa tattggcaga agaagaaggg tgtaatcaat
    58141 tatcggatct cgatggaaga gtctttatca tcgatcctat tgatggcacc ttgaattttg
    58201 tgattgaagg ggaaaatttt tgtattatgc tcgctgttta tgaatccggc gttggaaagc
    58261 ttggtttcat ttatgacgtg atgagaaatg aacttttttg gggtggaaaa ggaataggcg
    58321 tgtataaaaa tgatgagcca ttgcctagac cagcaaataa atccttatcg gaaggcttac
    58381 ttggtgtgaa tagttatctt ttcggtcata ataaatatgg aatccgagaa attgcggaaa
    58441 gcagcatggg gacacgtatg tacggatgcg ctggtcttga actagtggca atcttgaaag
    58501 gaaaccacat tgcttatatt tcaaacctta gcccttggga ttatgcagcg ggaaatgtct
    58561 tattggaaga gtttggtatg gtaacaagcg gtctttttgg tactcctttg acatttgaaa
    58621 aaagggaata ctatttagca gggacttctt gtgcatatgc agaaatcgca gaccttttga
    58681 aaaaagaagg ctgaaaaaaa ggtagaaatc attcacaaaa atgataaaaa cgagcaaaag
    58741 attaattttc tcgaaaaaat gaaaacattt tactatattt catgaaaaaa gtctgctata
    58801 attagcagtc gagtaaaatt tgccctgtca taaagaacca ataaaaaagg agttagttac
    58861 attgaattat agaaacgata tccgtaacgt cgcaattatt gcccacgttg accacggaaa
    58921 aacaacatta gttgatgaac tattaaaaca atcagataca ttagacgcac atacgcaatt
    58981 gcaagaacgt gcgatggact caaatgcgct agaaaaagaa cgtggtatca cgattttagc
    59041 caaaaacaca gctgtagact ataaaggtat tcgcgtaaac atcatggata ctccaggaca
    59101 cgcggacttc ggtggcgaag tagaacggat catgaaaatg gttgacggcg ttgtgttggt
    59161 cgtagatgcg tatgaaggaa caatgcctca aacacgtttt gtattgaaaa aagcactgga
    59221 acaacatatc acaccaatcg ttgtcgtaaa caagattgac aaaccttctg ctcgtcctga
    59281 gcatgtcgta gatgaagttc ttgaactatt catcgaatta ggagcagatg atgatcagtt
    59341 agactttcct gtgatttatg catctgcact aaatggtacg tcaagcttgt cagatgatcc
    59401 agcagatcaa gaaccaacca tggcacctat ttttgataca attattgaaa aaatccctgc
    59461 tcctgtcgat aacagcgacg aaccattaca attccaagtt tcattacttg actacaacga
    59521 ttatgtaggt cgtatcggaa tcggacgtgt atttagagga acaatcaaag taggagacca
    59581 agtagcatta attaaattag acggaacagt taagaaattc cgtgtaacga aattatttgg
    59641 tttctttgga ctaaaacgtc tagagattca agaagcaaaa gctggagact tgattgctgt
    59701 ctcaggtatg gaagatatct ttgtcggtga aacagttact cctgtggatc atcaagatgc
    59761 gttacctatc cttcacattg acgaaccaac attgcaaatg actttcttag ttaataattc
    59821 accatttgcc ggtcgagaag gtaaattcgt aaccgctcgt aagattgaag aacgtttgat
    59881 ggcagaactt caaacagatg tatccttacg agttgaacca acaaactctc cagatgcatg
    59941 gacagtttct ggtcgtggag aattgcattt gtctatcttg attgaaaaca tgcgccgtga
    60001 gggatacgaa ttgcaagtat ctcgtccaga agttattgaa aaagaaatcg acggtgtaaa
    60061 atgcgaaccg tttgaacgcg tgcaaatcga tacaccagaa gaatatatgg gtagtgttat
    60121 cgagtccttg agcttacgta aaggtgaaat gcaagatatg gtccatacag gaaacggaca
    60181 aatccgtttg acattcttaa cacctgcccg cggtttgatt ggttattcaa ctgaattcct
    60241 ttcaatgaca cgtggttacg gaatcatgaa ccatactttt gaccaatatc tgccaatgct
    60301 tcctggtcaa attggcggac gtcaccaagg ggcgcttgtt tcgatcgata caggaaaagc
    60361 aacgacttat tctatcatga gtatcgaaga acgcggtaca gtcttcgtag aaccaggtac
    60421 agaagtatat gaaggaatga ttatcgggga aaacagccgt gataatgatt tgactgtcaa
    60481 catcacgaaa gcaaaacaaa tgacaaacgt acggtctgct actaaagacc aaacatctgt
    60541 catcaaaaaa ccaaaacaat tgacgttgga agaatctttg gaattcttga atgatgatga
    60601 atattgcgaa gtaacaccag aaagcattcg cttgagaaaa caaattttag agaaaaatgc
    60661 tcgtgaaaaa gcatctaaaa agaaaaaata atctgtaaaa ggatgaagag agaaaccagc
    60721 ttgttataaa aaatagctgg tttctttttt tgtccaactc ttttctgtgg agaagtcatc
    60781 tttgtcaatg gacataaatt cagaaaatac gccgaaagac gtttaaaaga cttttaaaaa
    60841 acttatttct agagagtttt tgtttcataa taagataatg tttcacgaaa ggagatcaac
    60901 ttgtccttcg aagaaaatcg aagtaaaatg gcaagagaaa ggagcgggaa gatgaacgac
    60961 ttattaaaac aatactttgg ctatgatgag tttagacctg gtcaaaaaga aattatccag
    61021 aaagtcattg atcaggaaaa tgtattagga atcatgccta ccggaagtgg gaaatcaatc
    61081 tgttaccagc ttccagcttt actattagat gggcttactg tggttgtgtc ccctttaatc
    61141 tctttgatga aggatcaggt ggacgcggcg aatcaattag gtatacccgc cacttttatc
    61201 aatagctcgt tagatggtta cgaaacagct agacgcttcc aagaaatcga tagacagcag
    61261 taccgtttac tttatatcgc tcctgaacgt tttatcatgc cggattttat ccaagcaatg
    61321 aagcgatgga acgtccgtat gatcgctatt gacgaggcac attgtatctc tcagtggggg
    61381 catgatttcc gcccaagtta tttacagatg gcgaaccagc tagatcaact gccaaatcgt
    61441 cctgtaattg ttgctttgac agctacagcg actgtgcaag tagctgctga cataaaaaga
    61501 cttttaaaaa ttccagaaaa caaccatatt cagactggtt ttgaacgaga aaatctacga
    61561 tttcaagtaa ttaaagatca aaaaaaagaa caatatctaa tcgaatattt aaagatcaac
    61621 aaaaatcaat ctggtatcat ttatgcagca acgagaaaag aagtagaccg tctctatcat
    61681 ttgcttaaaa aattcgattt ttccgtgggt agatatcatg gtggtctaaa tgagaatgag
    61741 cgaacagaaa tgcaagaagc ctttttatac gatcgcttgc agctgattgt agcgacaaat
    61801 gcatttggta tgggtataaa taaaagcaat gtccgttttg ttatccatta tcaaattcct
    61861 ggctctctag aagcatacta tcaggaagct ggtcgggcgg gaagagatgg tctttccagt
    61921 gaagccatcc tgcttttctc tcctcaagat atccaagtcc aaaagttttt tatccagcag
    61981 tcccaaagag aagaagggca aaagcaaaaa gaatatgaaa aattaaaagc gatgacagaa
    62041 tacgtgtata ttgagtcctg tcttcagcaa tacatcctaa actattttgg cgagacaagc
    62101 agtccgtgca accgttgtgg taactgttta gatgacagag aaatagtaga agtaacgaca
    62161 gaagcacaga tggttctttc ttgcttaaaa cgtatgggtg agaactatgg taaacaaatg
    62221 ctgatgaaag tcttagcagg atcgaaagaa caaaagctgc aagctttagg ttttggacat
    62281 ttatctacct atggactgat gaaaaaccaa tcgcaaaaag aaacgatgca gttaattgaa
    62341 tatttgatat cgaatggcta tcttctgacc atcaatggtg agtatcctgt tttgaaagta
    62401 acagaaagag ggatacaagt attgaaagga caagaatctg tttatcgaaa ggaacccaag
    62461 aaagtccagc agctatcgga tgaagaaacc gatacactat ttgaagtgct gcgggaactg
    62521 cgaacggatc tagcatcaga agcaggcgtt cccccgtatg tcgtattttc agattccaca
    62581 ttgaaagaaa tgagccgaat acgtccatca agcagattgg agatgcttca gataaaagga
    62641 gttggccaaa gcaagcttga taaatatggc gaagcctttt tatctcggat aaaaaatgcg
    62701 gactctaacg ttctggataa gtagaaagaa cgaatacgta aaaatgaata aaaacaactg
    62761 gttaaatctg ttttttttgc tccttatgaa aatcaaatat gttataatta aggaattaga
    62821 atgggaggta agcacagatg gattctttgt cgaaaaaact tgcgatccaa gtattaaatg
    62881 atgaagcaga acggatcaaa cgattaatcc gaaatcaaaa caacagtctc tgtatctcac
    62941 aatgcaaagc cttcgaagaa gtggtagata cacagatgta cggcttttcc cggcaagtcg
    63001 gttttgctgt ccgtatagga gtaatcggaa agaacgaggg ccagctgatg ttaagtgagc
    63061 tagaacgaga attgaatcag ttatatagtg aggtttacca agaaatttat gataaaaaag
    63121 aagttggcaa ggaggaatag acttgcctaa gaaagtgaaa aaaaggcatc tattagacta
    63181 tagcatcttg attccctatt taattttatg tgtattggga ttgatcatgg tatatagttc
    63241 tacgtcgtat ctattattag aaaacggaca aaatccgtcc gcatcagtca ttaaccagag
    63301 tattttttgg gtgcttagct tgattgctat cgcactactt tataaaatga aaacagatgt
    63361 tttgaaaaac cagcgtttga tcatggcagc catcgcagtt ttgacgattt tgctgctgat
    63421 tgttgtgttt tttggtaagg agatcaatgg ggcgaaaggt tggctgcaaa ttgcaggatt
    63481 ctcgatccaa cctgccgaat acctaaaaat catttctatc tggtacctgt ctctgacttt
    63541 atctaaaaga caaaacagtg tacaaaaaga ttttcttggg actgtgaaac gtccacttgc
    63601 aatggttatc ggacttactg cattagtcgc tattcttccc gattttggta atgcggcagt
    63661 tatctttttg attattttgg ttctgctttt agccagcgga gtcaattatg tgtatacttt
    63721 gattgtagga gtaggaggct tttgtctaag tacttttacg atctggctga tcaatatcac
    63781 aaacggaaag atcttacccg gacgtttaca gtatatctat aatcgatttg ctatttacca
    63841 aaatcctttc tcagacgaat taaataaagg gcatcaacta gtcaacggct actatgccat
    63901 gttcaacgga ggactatttg gcaggggatt aggtaacagt atccagaaaa aaggattttt
    63961 acaagaagca caaaccgatt ttatttatgc aatcgtagtg gaagaactgg gagtgattat
    64021 ggggattttg atattagcgt tgctattctt tatgattgtg cgcattgtcc ttgtaggtat
    64081 ccgttcaaaa gatccgttca attcgctttt gtgcattggg atcggcgcaa tgtttttgat
    64141 ccaagtattt gtcaatcttg gaggaattac tggcgttatt cctttgacag ggatcacgtt
    64201 cccattttta agtcaaggag gctccagttt gctgatgctt tcgatttgtg tcggctttgt
    64261 cctcaacatc agtgcggatg aaaaacgcaa gagcttaggt ttataataaa aaaagatgta
    64321 ggagtggttc gatgaaaaaa gttttagttg ctaacagggg tgaaatcgct gtacgtgtat
    64381 ttcgtgcttg tacagaatta ggaattaaaa ctgttggaat ctatgcagaa gaagatgaat
    64441 attccgtaca ccgtttcaaa gcggatgaag catatttagt aggacaagga aaaaaaccga
    64501 ttgatgcgta tctagatatt gaagggatca tttctatcgc gaaagaatgc ggagctgacg
    64561 ctattcatcc gggttacggt cttttgtcgg aaaacttgaa ttttgcgcaa cggtgcgaag
    64621 aagaaggcat catttttgtt ggacccaaac tgcatcattt agatattttt ggagataaaa
    64681 tcaaagcaaa agctgcagct atcgaagcgg ggattgcttc gattccaggc acagatggac
    64741 caatcgcttc tatagacgat gccttggagt ttgcgaaaca atacggttat ccgattatga
    64801 tcaaggcagc tctaggcggc ggcggacgcg gcatgcgtgt agctcacgat gaaaagagtg
    64861 caagagaggg ctacgaacgt gcgaaaagcg aagcaaaagc agcctttggt agtgatgaag
    64921 tgtatgttga aaaatacatc gctaatccta agcatatcga agtacagatt ctcggggata
    64981 cgcatggaaa cgtcatccat ctttttgaaa gagattgttc tgttcagcgc cgtcaccaaa
    65041 aagtagtaga agtagcgcct tgtgtctcta tgaatgaaca gcaacgtcaa aaaatctgcc
    65101 aggcagctgt ccaattgatg aaatatgtcg ggtatgtcaa cgcaggtaca gtagaatttc
    65161 ttgttgaagg agatgatttt tactttatcg aagtcaaccc tcgcgtccaa gtggaacata
    65221 cgatcacaga aatgattaca gatattgata tcgtaacaac gcaactgttg attgctcaag
    65281 gtttggatct gcataaagaa atcggattgc ctcagcaaga aggaattaaa ttaaatggtt
    65341 cagccattca atgccgaatc actacagaag acccgttgaa taacttttta ccagacacag
    65401 ggaaaatcga tacgtatcgt tcacctggtg gttttggcgt acgtctggat gtcggaaatg
    65461 cttacgcggg ctatgtagtg acaccgtatt ttgattcatt gctagtaaaa gtatgtacgc
    65521 atggtgctac ctttgaaaca gccattcaga aaatggaacg ttgcttgaaa gaatttagaa
    65581 tccgtggagt gaaaacaaac attccattta tgctcaatgt catcacacat cctgaattcc
    65641 aatcaggaaa tgcaaaaaca accttcattg atagtacagc aacattattt gaatttcctc
    65701 gactacgaga ccgcggaaac aaaacgatga aatatatcgg tgaaattact gtgaatggtt
    65761 ttccaggtat cgaaagtggc gaaaaaccat tttatgaaga accacgtatg cctaaagatc
    65821 taatcacaag aactgattac gtaacggcta agaatgtatt agatgcgaag ggtgccgatg
    65881 ctttggtcga atggatcaaa ggacaggaaa atttgttgtt gacggatacg acatttagag
    65941 acgcccatca aagtctgcta gcaactcgtg ttcggacaaa ggatttcaag cagattgcac
    66001 gtttgactgg agaaggtctt cctgaactct tttcaagtga gatgtggggg ggagcaacat
    66061 ttgatgtggc ttaccgcttt ttgaatgaag atccttggca acggttgaga aaaattcgtt
    66121 ctttaatgcc taatacgttg ctgcaaatgc ttttcagagg ttcaaatgcc gttgggtatt
    66181 ctaattatcc tgacaatgta ttggtggaat ttgtcaaaga agcagcagcg caaggaatcg
    66241 atgtatttcg aatctttgat agtttgaact ggactccaca aatggaaaaa agtatccaag
    66301 ctgtacggga tactggtaaa atcgcagagg cagctatttg ctatactggc gatatcaacg
    66361 atccaagtcg ggcaaaatac aatgttcagt attataaaga catggcaaag gaactagaac
    66421 aactgggtgc gcatatcatt gcaatcaaag atatggctgg gctgcttaaa ccgcaggcag
    66481 cttatcgttt gattagtgaa ttgaaagaaa cgacagatct gccaatccat ctgcatactc
    66541 atgataccag cgggaatgga atcatcactt attctgctgc ttcaaaagca ggagtagaca
    66601 tcgttgacgt ggcaatgagt gcaatgagtg ggaatactag tcagcctagc atgagcagct
    66661 tgtattatgc actggtcaat ggtccacggc tgccagaaat cacgattgaa aatgcacaga
    66721 aattaaacca ttattgggaa gatgtgcgca tgtattacaa accgtttgaa aacggcttga
    66781 atgctcctga aacagaagta tatatgcatg aaatgccagg aggacagtat tcgaatctac
    66841 agcaacaggc aaaagctgtc ggattaggtc atcgctggga tgaaatcaaa caaatgtatc
    66901 atactgtcaa cttgatgttt ggcgatatcg ttaaagtaac tccatcatct aaagtagttg
    66961 gagatatggc actatttatg gtccaaaatg atcttacaga agaagatatt tatgaaaaag
    67021 gcgaaacact aagtttccct gaatctgttg tgactttctt ccaaggagaa ctagggcaac
    67081 ccgttggcgg atttcctaaa aaattgcaaa agattattct taaaggcaga cctgcattga
    67141 atgaacgtcc aggtcttttg gctgaatcag tcgacttcaa cgaagtaaaa aaagaattgg
    67201 cagaaaaaat tggctatgaa ccaaaacaag acgaagtatt aagctatttg atgtatccgc
    67261 aagtcttttt ggactatcaa aaagcatata atcaatttgg cgatgttaca ttactggaca
    67321 caccaacatt tttccaaggt atacgtttgg gtgaaacaat caatgtacaa atcgaacgcg
    67381 gaaaaacgct tatcattcgt ttagatgaaa tcggagaacc cgatatcgaa gggaatcgcg
    67441 tactgttctt caatttgaat ggacaaagac gtgaaatcac tatcaaagat aattcgatca
    67501 tcagtacggt ccaaacccga ttgaaagctg aaccgacaaa ccgagaacaa attggagcaa
    67561 cgatgtctgg ttctgttttg gatgtattag tgaaaaaagg cgataacgtt aaaaaaggtg
    67621 atacgttgat gattactgaa gcgatgaaaa tggaaacggc catcgaagca cgttttgacg
    67681 gagaagtcgc acatgtatat gtgtcttcag gagacactat ttcgtctggt gaccttttga
    67741 ttgaagtgac tgagaaataa taagcgaaag gtggggaaac atgaaaagac tgggtttgtt
    67801 ttggctgatt ctgctattag ttattgtggc attctacatg gaaccagtct tgtttccgcc
    67861 aaaacttgaa gtgaaggaaa actctgatca acaaatatca aaccaaatga caacgtggaa
    67921 ataccaggag cttaaagcaa ctggtttttc tacttatatc ggaaaaccga tcgatcagct
    67981 ggaaaaggat tttggccaag cctatgatcg attaactagc ggatttggtt ttgaaacaag
    68041 atattatgaa aataaagcta gtcatctcac atttgaagcg aacatccaag acagcagagt
    68101 cacaactgtt aaagtactgc aaacagggcc tgctgatatc gcgccgtttt atttagggat
    68161 gacgatgcaa gatcttacga aaatcactac tatctatact aatttcactt ttgagtacca
    68221 tgatacagaa gttggaatcg aactaatgga agaggatatg aattatcgtc cgctaattgc
    68281 ttttgataac gacacttttg ctattttatt ttttgatcaa acaacaaacg gcctattttc
    68341 agttgtgtat ttagataaag atagtctttt gaaactattg ccatatcaag tgtttggtga
    68401 aggccttcca cattatcaag cagataaatc ggctgattgg gaatcaatca atcgcgttaa
    68461 agaacgaaaa agtacgactt tattaaatat gcttagaaga gaagacgaac ttcccatata
    68521 caatcaaaca cttgcctttc aaaaccacag tcagctgcta ctacatgatt ttttgacaag
    68581 gcctgaggaa atattgtcag aggaacgtat ggctgattgg caaaaatcgc taggcagtgc
    68641 tcaaacgaca gtgaaattct cactgacgaa tgatgaattg gagaagttaa tcagtaaaca
    68701 aaaattagaa aatactagtg ggctatttat tcatccggcg attgacgcta ctttttcgtt
    68761 tttatattgg tacagcgatc cttacaacca tgaccgtttc atggacccgg gaacggattc
    68821 tgtaggcatt gctttttcta aagaaaatat gttagtctta ttgcaagaag aaaacggaga
    68881 aagtagtgat agcagtgatc gttaacgaag agctgtttca actggaggat caatgcctcc
    68941 aagtcgctga aatgataaaa aagagtaaaa cgatggcttc ttatcttgaa cataaacagt
    69001 caatggaaga atctgcaaaa gtttgccagt tgaaagaagc gttcttgtct aagaaagaag
    69061 catttgaaaa tgtagcggca tatggtgagt atgcacctga ttatcgtgaa aaaaaacgag
    69121 ctgttcgaaa agcaaaaaga cagcttgata tggatgagca tgttgcagct ttccgatata
    69181 gtgaaacaca gctgcaaagt ctgttggatc aaattgtcca agtacttgcc gaagaagttt
    69241 ctaatgaaat caaagtagat gcaggtagcc cgttttttga aacgaaaaaa cactttggat
    69301 gcggaggtaa ttgtcatgca attagacaat agtgaatttg aaataacaaa acgacgcggg
    69361 ctgatcgtat gggtctacag cttaaaacaa ttaaagaatc tgaaaaagtt cggcttgttg
    69421 cattatgtct caagaagaat gaaatatgtc gtgctttact taaatgaaga gacattggaa
    69481 gaaacagaag aacgaatccg taaattacat tttgttcgaa acgtagaacg ttcttaccga
    69541 ccggatatcg aaatgaattt tgccgaaaaa ataggaaaca aaacagtatt aaaagacgaa
    69601 ggtttcgaaa ttgaagagct aaatacagaa atccgattag ctgaacatct ttaaaaatct
    69661 cttatgagca gtttttaaat aaaaaataac ttggataacg atcgattttt gatcattgtc
    69721 caagtatttt tttggtatac tagtaccagt accatttcta aggagtgagt agatgcggat
    69781 aatatcagga aattatggcg ggcgccgttt aaaaagttta gcaggggcga acaccagacc
    69841 aactacagat aaagtaaaag aatccatatt caatatgatt ggaccatatt ttgatggaga
    69901 gacagtattg gatctgtttg caggaagcgg tggtttagct atcgaagcag tatctagagg
    69961 ctgcagtcat gcggtatgcg tagacaaaaa ttaccaagcg ctaaaaatca tcaaagaaaa
    70021 catcgaaatt acgaaagaac cagagaaatt taccacaata aaacttgatg cggacaaagc
    70081 aatcgaacgg ctagcagcgg agaaacaaca attcgactac ttgtttcttg atccgccata
    70141 tgcaaaacaa aaaataatcg atcaaatcca taaaatggaa gaactgggac ttttttctaa
    70201 aaccgcagtt atcgtatgcg aaacggataa aacagtcgaa ttgccagaaa ctgtggctgg
    70261 attccatcaa ataagaaaac agatttatgg cataacggcc gtaactatct atagaaaaga
    70321 gggaaatcaa tgaaacgtgc attattccca ggaagttttg atccttttac aaaaggtcac
    70381 ttagatacgg tggaacgtgc ggctaaatta ttcgatgagg tagtaattgg tgtttttatc
    70441 aatacttcaa agaaaagttt gtttccccca gaagaaagaa tgacattgat cacaaaagct
    70501 gtttctcatc ttcctaatgt aaaagtcatg catcaagaaa atcaattgac ggtagaaaca
    70561 gcaaaagaga ttggtgcaga tgcgttgatc agaggcatac gaagtatcaa agactttgaa
    70621 tatgaacgag agattgctca gatgaatcat catctatatg atgaattgga aactgtcttc
    70681 ttattagcaa aaccagaata tagtcacgtg agttccagta ttttaaaaga agtactgcac
    70741 tttggcggag atgtgagcag ctacttaccg ccagtgatca acgaagcttt agctggaaag
    70801 cgtgaatcaa atgaaagcta aacaatggtt gaaaacagta ctgattatca tatttggaat
    70861 gttgatcatt ggatttgtag tagttccctt gccgtattat gtagaatcac cgggagccac
    70921 gatcgatctg aaagaactaa ttacggtcaa tgataaggaa gataaaggtt ctgggtcttt
    70981 ctctcttacc tctgttggta tcaggcaagc tacaggtttc ttagctttaa aagctaaaat
    71041 gacccctttt gaagaattga tcagtgaaga agaattgact ggtggggcga caaatgaaga
    71101 atataatcaa atgcaacagt attatatgga ttcttcccaa aatgcagcaa tcgaacaagc
    71161 gttaaaactt gcagacaagc cttataaaat ggaattcaaa ggcgtatatg tcatgagtgt
    71221 agagaaaaca tcgaatttct ttggcaagct ttcagttgga gatacagtaa caaaagtaga
    71281 cgggaaaagc tttcaaagta cagaagcatt tatggattat gtaaaaagcc aaaaagttgg
    71341 acaaacaatc gaaatcagtt atttgcataa tggtgaagaa aaaacagctt ctggtgatct
    71401 gatcgagtta ccaacagata aaaaggcagg aataggtatc acgcttactg accataccga
    71461 gatcgaatca gatacagatg ttcgtattaa ttctggctct atcggtgggc catcagcggg
    71521 attgatgttc actttagaaa tctatgaaca agtcactggt aaaaacttgc gacatggtaa
    71581 gcaaattgca ggaactggaa cgatcaacag tgaaggagaa gttggccgaa tcggaggaat
    71641 cgataaaaaa gtggcaagtg cggataaagc tggcgtagag attttctttg caccagatga
    71701 tacgatttca gacgatgtta agaaagaata tcctgaaata aaaaccaact acgaagaagc
    71761 aaaagctgcc gctaaaaaat tgaattcctc aatgaaaatc gtaccagtaa aaacagtaca
    71821 agaagcgctt gactatctag agaaaatgaa atgaaactaa ataattaaag aggggttgtg
    71881 acacgagttc tgtcacaacc cctctttttc gtcaaaaatc ttcacgataa aaataaaaac
    71941 aaaagaaaaa attgacgtaa ttagatgttg aagggacgat tttgccagag ctcactgttt
    72001 cttcaatttt aaatgaaagg agtaatgaat gatttttact ggtcattctt tgaaaaaaga
    72061 acaatgatag aaaaaatcag caagaaaata ctagcttcac ccaaattgat catttggatg
    72121 tctatgatag ttcttatctt ttgtatcgca agtggttttt tattatcaaa tttgctattt
    72181 tatagagaaa cagaagagac tgctgcaaaa gaaatacttt cttcgtttag ttccagtgga
    72241 gaagatccat cagctgaagg aaacgatgta gaagaggatg ctcgtccaaa ggtcatgtac
    72301 accgatatca aaggaagtgt gaaggagcct ggtatctatt ccttttcttc agaagaacgg
    72361 gtctatgatg tactaaagcg tgcaggtgga ttattagaag aagcggatag cgatcgaatc
    72421 aacttttctg cgaagataga agatcagcaa gtcctatata tccctgctgt aggtgaagag
    72481 ctgccagaac atcttgatca gttggcatcc tcggaaggaa aacagcctac agcagattcc
    72541 gagcctccca aaataaatat caatacagcc actccatcag aacttcagca gattcctggg
    72601 atcggatcag tcaaagcgca agaaatcatt cgatttagag aagaaaacgg atccttccag
    72661 aaagtggaag acctgcagga gatttctggt atcggtgaaa agacagttga aaaactaaaa
    72721 aacttcgtta caattaaata gaatgattca aaatcgactg gcacgagcaa taaatggacg
    72781 ctaatggata ctaagaataa tccaattcta agagcttggc aggacgagga ggtagtattt
    72841 ttggatagga aatagcttga ttgttcgcaa agaaacatta aaaaaaccaa tgctgaatgt
    72901 ctatctccaa aataaaatat ctgggattca tattatgaat acggctgtca gcggcaataa
    72961 cagtcaagct ctacgagaac gctttgccaa agatgtactt agctatacag cagataaagt
    73021 atttattctc attgggacga atgatctagc agagcataaa caactttcta aagaaacgta
    73081 ccaaaaaatc tgcagtggat gatagttcag ctgaaaaaag actatagttc tgatcatgct
    73141 ttctttatca cgccaccagc agtcgatgag acaaaacaaa aatatcggaa taacaagttg
    73201 atcaaagaat atatctctgt tctcacagaa gtagcaaaac gccatgattg tgaagtatcg
    73261 atttttatac ggaatttctt gaacaaatgg attcgaagaa tttttataat gggctaatgg
    73321 atgacggact tcatttcggc agtgaagggt aggatctttt gtcagacagg atgattcgtt
    73381 atatttaaag aatattttgt ttaagcaatg agtacttttt aatcaacttg aaaaaacgga
    73441 ggaatataaa acatgttaga aaaatcttca aaagcatgcg atacgaataa agaaaaagtt
    73501 actgataagt cacataatga acgcattcct tgggaccagt actttatggc tcaggcagta
    73561 ctgttatctt tacgtagtac gtgtacacgt ttagaagtag gagcgacact cgttcgagaa
    73621 aaaaggatca ttgctggcgg ctataatgga gcagtcagtg gagatgtcca ctgcatagat
    73681 gaagggtgtt acgtcgtgga tggacactgc ttgcgaacga tccatgcaga gatgaatgca
    73741 ctcctacagt gtgcaaaact tggtatcccg acggaaggtt cagaaatcta tgtcacacat
    73801 tttccatgtt tggcctgtac aaaggctttg ttgcaagctg gtgtgaaagg gatcaattat
    73861 ctccatgact atcgaaatga tccttatgcc caagcgttga tcgaacaatt aggtgtatct
    73921 gtacaccaag ttgctttaga ctcacagcat ttttcaaaac ttcaaaatga attaacagga
    73981 aaacatgtct agcgttttag aaaggcatgt tcgaaatcgg ttgatttttc cggcaattac
    74041 gtcgatgatt atctgtttgt tgtattacac tgacaattgg ggttatcgtt tgcttcttgc
    74101 cggaattttt ttatggtttt cgctaaaaga aggacgtttt atcgcgtact tgtgtatttt
    74161 gtgtagcata acggtatcca tttcttatag gctacaagag tattcatttt tagaggaaaa
    74221 aacaacatct gcgcaaatta ctgtatttac agatacgatt cgtgcaaatg gtaatttttt
    74281 gacaatggaa ggaaggttat cagagaataa acaaaagctc cagctttctt atcaagtaaa
    74341 cacagaagca gaattgaaaa catggcttaa cttccgagga aagggaatag aattgactgc
    74401 tgaaggcgag ttcatctctc cagcaaaaaa gcggaatctt tataattttg accaagaagc
    74461 ttacttcagg aaccatagaa tttctggacg gttccaaatt caaaagattg aacagatcac
    74521 catgcaaaat cactggcgaa actggcttca gagaaaaaga ggatctttta tctcctttgt
    74581 ccaatctgaa tttccgcaaa aaacaagtgt atatattaat tctttattat ggggctataa
    74641 agatgcagga ttcagagaac cagaagaaat ttttagggag agtggtttgc tgcatctgtt
    74701 cagtctatcg ggtctacaca tccaatttta tttagggtgg ctgtattatt tatttcgacg
    74761 tatcggatgg ccactacacg tcagtattat tccgttgagt ctactaacta tctgttatgt
    74821 ttctattgca ggaagttcta tttctgtttt gcgtgctagt ttgctgtttt tattgcgatt
    74881 gctagtcaaa ttgctgaata agcattattc caccatggac ttatttgcag ttgtcttttg
    74941 gcttctttta ctatttgatc ctcgatttct ttttcatgcg ggtggacagc ttagtttttt
    75001 catgagtttt ttgtttatcc tgatttcttt tgatggagat tggttgaaaa aaacggcctc
    75061 tcacgtcctg tttactatga tcacgatgcc gttgattgta tggcattttt atgagtggcc
    75121 tattttaggt agtcttttta cattactgat tgcaccggta ttcaagtttc tttttcttcc
    75181 tggtgtgtta tttctcacac tcttcaatcg ctggctgccg gaaactttac ttttgctgat
    75241 agaagaggga ttaatcttat ttgagcgaat aatcgatttt tcttcagggt cgtctctcat
    75301 tataggacgt ttgccccttg tgctatctgg gatgtatatt ttcggattac tgatttgtat
    75361 ggataggttg aaagaacaac ctgtgaaaca cttgttttat gttggtcttt tttcttttat
    75421 ttttttgact ttgccatttt tacgcttttc ttcaacgatc gcttttatcg atgtcggtca
    75481 aggagacagt atctttttgc aaagtccttt tcgaaaagaa acgattttga tcgatactgg
    75541 aggtcagtta cactttgaaa gagaaaagtg ggcgcaaggt atgatacgtc ctcctgctga
    75601 aagaaatatc gttccatatc taaaaggtca ggggataagt gtaatcgata aactcatctt
    75661 gacacacgac gatacggatc atgtgggaga attacccacg ttatctcagc attttactat
    75721 caaaaaaatt tatatcggtt ggggcgctgg acgaaatgag ccattacaaa ggcatttatc
    75781 tgaagctcaa aaaaatggta ctgagatcat tgaaatcaaa catggggaca ggatcagcgg
    75841 ttattatgat ctttatgtat tgactccttt tgaaaaggga gagggaagaa atgaagattc
    75901 gattggtcta tggatggaat ataacaatag gcgcttttta tttttaggag atttgaatca
    75961 agcaatggaa aaacaattgc ttttgattta tcctaatctg aaagccgatg tagtcaaatt
    76021 aggtcatcac ggtagccgta cgtcaagtaa cacagatttt ctttcccata ttgaagcaaa
    76081 acatgggatt atatcctgcg gcgtaaataa tcgttatggt cacccgcaca gcgaagtatt
    76141 ggagacacta gaagaaaatc agataaaaac atggcgaacg gatcaacaag gaatgatcac
    76201 ctatcgctgg catccagttt ttcatcctca tgggctagtg gaaacaatga tagactagta
    76261 attctcatct gctcatggta ggataatttt tgaaagtggt gagtaatttg agtatacaag
    76321 cagagttaca aaaaatacgt aaagaaaaac ttgcatcttg ttacttgatt ttaggaacag
    76381 aaaagtttct ccaagatcaa gttcggacag aaattgtcaa aaaaatccaa ttagagggcg
    76441 aagatgactt aaatttttta agttttgaca tggaaaatgc aagtttagat gaggtagtag
    76501 ctgaagcaga gacgttgccg tttttcggag atcaacggtt agtattcgta gagaatcctt
    76561 attttttaac aggtgaaaaa gtaaacaatg gaatcgaaca aaatactgat ttattgacgg
    76621 cttatttgaa agaaccatta gaatcgactg tattagtatt ctttgcgcca tatgaaaagt
    76681 tggatgaacg gaaaaaagta acgaaacaat tgaaaaaaac agcaattatc atcaatgtcc
    76741 aacagttgaa tgaaaaagaa gtacgtcaat atctgatgaa taccttagag aattcctcaa
    76801 tcaaaatgga acgaagtgca attgatttat tcttgaggtt gaccgactta gatttatcca
    76861 aaatgatgag agagttacag aaattggtcc tttatggaca aaatcaacag caaatcacgg
    76921 taagagaagt agaagaatta gttcctaaaa cgttagaaca taatattttt gacatgaccc
    76981 aatatatttt aaaagggaag acggaacaag ctttgcgttt atacgaagat ttagttatgc
    77041 aaggggaaga aacaatcaaa attaatgcga ttttgctttc tcaattacgt ttatttttac
    77101 agacaaaatt tcttgtaaag attggttatc agcaggcaaa tattgctgaa acattgaaaa
    77161 tccatccata tcgtgtaaaa cttgccatgc aggaagtaaa aaaattcgat gaacatttgc
    77221 tggtacagtt gtttgatcaa ttagttgaga tggattatca aatcaaaacg ggacaaatcg
    77281 aaaaagaatt aagttttcaa ctattcgttt tgcgcacagg gcagttgtta agaaatcgat
    77341 aagaagaaag acgagggcaa agcatgcggg aaggtcgttt gtttttgtcc tttttttgac
    77401 aaataaaaaa atcggccgaa gccgattctt ttatttagcg atttttttgc ttaaacgaga
    77461 tttgtcgcgg ttagctttgt ttttgtggat tagacctttt gattcagcca tatcaatagc
    77521 tttaacagct tctttatata acgcatctac gttgtcagca cctgaagcta cagcatcttc
    77581 aaattttttg attgcagtac gcatagcgct tgtttgagat gaatttttca cattagcgtt
    77641 ttcgcttgta cgaacacgtt tgattgcaga ttcaatgttt ggcattagtt tcacctccga
    77701 taacttaagt tcttacgcgt gttaccgcat aattcaacat tcattattat acctaaaggc
    77761 agcgtgcttt gcaatatgaa catgacaagt ttttttcctt aacagtcttt tattaggatt
    77821 ttttttcagt aaaaacggat agtacaagct gaaaaaaatc aaaaagacgc tattgtggga
    77881 ggcaatagcg tcaaaccaaa ctagattttt atttatctat ttaaacaaag tttgaatggg
    77941 aatgtttggt actaagttat tatataacaa agagaaccgt ttgcaaagtt ttttttgaaa
    78001 agggatacga ttccctgttg ttctttgttt ttctattaag aaactgcagt tattttataa
    78061 ccagtatcct ttgatcagta aaggctattc tggtgtaccg attattttaa tgtattcatc
    78121 gatatttttt atcaacattt tttttatgac caactggttc cgacggttcc atttgccact
    78181 tacttccagc acatatttat ttaggggcaa acgtaagata tattttgcca aatagcgatc
    78241 tttaagcagg ccattgatca cacggtcctc aagctgcaag ttgatcccag tcagcatcat
    78301 tggctgttct tgtaaaacgt acgtatcaat taaaagacct tcaaaagttt ccacaagcgt
    78361 cttcctttct tttcaaagta acctgaaaac aggcactaat tcttccattt tgatattcgt
    78421 tgaagtggat gcgatcattc tttcttttgt ccactgtaat ctagtataac aaaaaagagc
    78481 agaagagcca aaaagaaaaa agagcatcga attctatagg aagtctcatt ggcacggcct
    78541 aaattattac aaaacattga ttaaaaaaag cccctgcatc gcaggagcat cccctatatc
    78601 gttttaatag gtcaatcaaa aagcagtatc aaacacattt tgtatcaaat caggaaataa
    78661 tagataagct aagcccagaa tcaaaagtac cgctagaaga aaccagccga ttgttcctat
    78721 gaaatacttt aatttttcga acattgtatc acctcgttgt cagtatatca cggatcagta
    78781 aattgggggt gttaagaatc tcctaaaata gacaaaattt gttctatttt cctaaagcaa
    78841 atacttgtaa gatacagtaa gtgataccag aagcaatgac cggtccggcg gcgattcctc
    78901 ttaagaagac aacacctaaa atcgtgccga aaacaagtgc aacagtgatt tgtggatcag
    78961 aagcaatcaa tcctacaccc ttggaagata agacagccac tagcccaccg caaaaaacag
    79021 ctacccagcc aataggggat ttaaaggctt ctaatagatc acgtagaccg atttctcctg
    79081 ttgcgatagg gattagaata gccgctgtga tgatcgtcac cccaatattg attcctttgc
    79141 cttgaagcca agggaaaaag gaacgggagg ctggtatgag cttcataatc aaaacaaaag
    79201 ctgccgctat catcagactt tgatttttag ctaaaattgc tacaagtaaa ataattccta
    79261 aaaaaagcca actttccatg aaatatattc ctcactttct tcgaaacaat cataacatgt
    79321 ttttagaaag ttacaaaagt aggaattcat ccttcaagac ctattcataa gctgtttttt
    79381 tcataaaata aacaaaatca taaaaaaaca ctttgcttat ttgacagaag acagccgcgc
    79441 tttgtatagt ggacaagact tacatttttt tgaagaatca gaaagatcag gtaaaggtct
    79501 tagtaaacgc aagccattca gaataaccaa tattgtgctt ccttcgtgtc ctacaactcc
    79561 aagaggtaga tttaaaaatt gcaggaagtt agaagcaatc aacaagagaa taacaagaat
    79621 cgaaaaaata atattttgtt tgatgatccg ccgcattttt aaagatagcc gatgactgta
    79681 gacaaattta tttaagtcgc ttttcattaa gacgatgtct gcgatatcca tagcggcatc
    79741 tgtcccttgc cccatagcga cacctaaaga agcggctgct aaagcaggcg catcattgac
    79801 tccatcacca accatcgcat ttacttggta aagttcattc tcttttttga ctagttgtgt
    79861 tttttcttcc ggagtacagc ttgcatagta atgttcaatc cctacttgtt cagcaatggc
    79921 ctttgctgtg ccagaatggt cgccggttat catacttgta tggatgtttt gggatttaaa
    79981 gtaggaaatt acttgttggg tatttgcttt tggaatatct agcagaccta aaacagcaat
    80041 taactgctga tctttagaaa gataaatcac ggttttgcct tgattttcca aacgttcgat
    80101 catttccgca atctctttgc tgattatcat cgtctcagga tcatatgcgt gcttgccaac
    80161 tttccattgc gcacctaaat aggtagtttg taatccaaaa ccagtaattt cttctattgg
    80221 taagtattgg atttcttcag gtattgtgca atcgaaatat tgcaaaatcg catgagcaag
    80281 tggatgcgtc gttttttgtt ccatagcaac aagaagttgt tgtgccaaat ccttttcatc
    80341 aaagaaaaag ctgtcagtta caactggttt tcctttagtg agcgttcctg ttttatcaaa
    80401 ggcaatcgct tttaattcag aaagcttttc taaatggata ccgcctttga ttaaaatccc
    80461 attttttgca gaatttgaaa gagcagctag agaagctggt gtagcagatg cgacaagcgc
    80521 acaaggagaa gccacaacta ataataccat tcctcgataa aagctttcat cccaactcca
    80581 acctaggaaa aagtggggga gaaggatcat tagaggaaca gcaagcaaaa tcaattttac
    80641 ataattattt tcgattcgtt cgataaatga agctgtttga gttggcgtat tctgcgcttc
    80701 ttctactaat tggataatct tagcaaagac agtttcggaa ctcgtttttg ttactcgaat
    80761 cgtgatggga tttccttgat tcagcgttcc tgcatacatc tcatcgccta aatatttttc
    80821 tacaggcaca gattcaccgc ttaatgcaga ctcatcgatc gttgcctgtg tgctttctaa
    80881 aaagccatct atcggaacag ctgctccttt agcgacaaat accaagttat ccacctttag
    80941 ttgagaaaca tctacttctt gcatttgtcc attttctttt aataattggg ctttttgtgg
    81001 ttgaagattc atcaaagcag taatttcttt ttgactttta ttagttgtgt attcctctaa
    81061 tgcaccgctt aaacaaaaga tgaatgtcaa cattgctcct tcaaagtaat taccgatcaa
    81121 acaggcacca atagcagcta aagccatcaa taaatccaca ttcagtgtcc gatcattcca
    81181 caattctttc aagccttccg atgtttgttt atatccaccg atgataatgg cactgatata
    81241 aaaaagtggg gtgatggtaa gagagaatcg gtcgaagata aaaccgaaga tcataaataa
    81301 aaggcagccg attgttgctg caaaagcttt tttctgatta gtcatcgtta ttagacctcc
    81361 ttgtcaaaac aaggatagca taataaaaat gtaattgata ataataacag ctaaatgaga
    81421 acgataatgg ttatcatttg gtgattaagc ttgatgttgt ttttattatt tataatgatt
    81481 attattaaag aaaataaaaa accagaacat ttctcaaata agagaagtgt tctggttttt
    81541 tctacattgc aataaaccaa taaccaatca aaatgatacc aaggataata cgataccagc
    81601 caaaagcggt aaagtcgttt ttcttcaaat agcttagtaa gaatttgata gctaagacgg
    81661 aaaccacaaa agatacgaat gttcctacaa gaagaacaac agtttcttcc attttgaatg
    81721 aattcccttc agctaaatat ttgacgatct tcaagccgct tgcgccaaac atgacaggaa
    81781 tccctaagaa aaatgaaaac tctgtagcga caaaacgcga agcaccaatc aagatagatc
    81841 ctaggatcgt tgcgccagaa cgggatgttc cgggaacaag tgccaagact tggaataacc
    81901 caacaagaag agctgcttgg tacgtaaagt cattcagaga agtccaacga ggtgttttgt
    81961 ttttgttgcg tttttcgaca ataataaagg caataccgta aacgatcaaa acaatggcaa
    82021 ctggtaagaa cttgtggaaa tttgcttcta accaatcatc caaaggcaaa ccaatgatga
    82081 tggctggtat gcaagcaacg atcactttaa accaaagtgt ccacgtatct tttttttcta
    82141 ccgttgattt acttggagaa aatgggttta acttgtgaaa ataaagaacg acgacagcca
    82201 agatcgcccc taattggata acgacattga acatcgacat aaattgtgaa ctggcatcca
    82261 gtttgatgaa ttcatctgct aggatcaaat gaccagtact actgatgggc agccattccg
    82321 ttattccttc aataataccg agaatcactg ctttgataat atttgcaaaa aacataaatt
    82381 ccttccttcc caattaagat acatcttata gtataccgtt attttttgag aaacccaatc
    82441 tgttttttaa tattaagaaa aacttgagaa ataaagcaga taagtacacg aagagaaaaa
    82501 agaagacaaa gccctcgttc agactttgcc ttttatgatt ctctggtaaa aattaaaata
    82561 tccccattat agaaacgagt cgcgactatg tgttttcccg ttatcgatat aagacgtatc
    82621 attgatgccg tggatagaaa atcgaccgtt tgcataagtg acctttgtta cgctgccatt
    82681 taaaagccca gcacgaacag gctgtttagg atcgatcaga ttaagcaaaa aggcgatcgt
    82741 caaaccatga gatacgacta aaacatttcc accaccattt ttttcgcagt gatgggcgat
    82801 atcttcaaat cctgtccata cacggtctcg caatgtttca taagtttccg cccacccagc
    82861 ggtatctaag ttgtatacag cattagcaat ttgttcaaaa gaaacttctg tggtaaacat
    82921 ttcatcatat gttttaaaat tcaatacacg agggatcaca ccccacattt ccatgtcata
    82981 tccaccttca agagacccga aacaccattc tctaatcctt ttttcaataa aataaggaat
    83041 ttcttttccc ttagaatgtt ctcctagaat aatacgggca gtttcgattg cacgaccact
    83101 gtcactagaa taagcaaatg aaaagtcgac attttttaac ccgcgaccta aataatgaat
    83161 gccttcttgt ccttgctttg tgaggggtgt atcacaccag ccttgtactc gttcaattgt
    83221 attgaacatc gtttttccgt gtcgtacgat ataaagtgta gtttctgtca ttttattctc
    83281 ctcctaagtc gttttaacga ttgaaaaaag gatttgtcct tttttcctgt tcaatcgtcg
    83341 ttgcatcgcc atgtcctgga tagactgcaa attcatcagg taatacaaac agctgggtcc
    83401 gaatactatg aagtaattgt tctaaattcc ctgtatacag atcggttcta ccgatacttc
    83461 ctctaaacag ggcgtcgcca gaaacgacaa aatcatcaaa aataaagctg acgcttccta
    83521 tagaatgccc tggggtaggg acgacgcgaa aagacatgtt tcctaaagta taagttttca
    83581 tttcaaattc atattctgca ggcgagacaa taacatcagc aatatcatca tgtctaccga
    83641 gtccagaaag attcaaaatc ggatcaccta gccatttttg ctctagtggg ctgacataaa
    83701 ctgggatttg ataatacgcg cgtaaaggtt ccactgcacc gatatgatcg tagtgagtat
    83761 gggtaagcaa aatcgctacc ggctgttggt ttgttttttt gatttcttgt ttgattcgtt
    83821 ccgcatcttc tccagggtca atgatcaaaa gtgcttcttc gttgtagacc aaataacagt
    83881 tttcttcaat cattcctgtt tttaatcttt cgatatgcat gtgcattcct ccttgatagc
    83941 caaaacttct ttcatccatt agtataacgc attcattgct aggaaacaaa aacaggtact
    84001 tatttctctt tacttatctg aattatcaga ctatatgaaa agtatttgga tattctaaag
    84061 agaaggaaag taagttttca tagcatttta atatgtcctt gttacattag attcatccaa
    84121 aaatccaaat tctatatcct atatccctgc ctaatcagca gggtgttttt ttatgtttgg
    84181 aaagagagct ctttttgcta aactaggttt tttcaggtaa tcttagagag aaagaggtgg
    84241 tggaaatgat caaacagtca aaaatatctg tgcggcactt agtagaattt attttgcgac
    84301 gtggaagtat cgacaatcgt aaaaaaagta accatactgc attagaagga gcgagaatcc
    84361 accgcaagct ccaaaaagat gcgggagaaa actatgaaaa agaagtcttt ttaaaaacaa
    84421 cagttcaatt agatgaatat tacctgatcg ttgaaggccg tgcagacggt attttcaaaa
    84481 gagacggtat ttattatata gatgaaatca aaacctccga accacgattt gaagatttag
    84541 aaccagaaca agtggaactt ttctttcatc aagcaagggt gtatgcctat atctatagtc
    84601 aagaacatga tttacaagaa ataaatctgc aattgactta cttccagacg gcagaagaag
    84661 tgatcacgag aaaggttgaa catcagacaa gagagcagtt agaaactttt tttaaaaagc
    84721 tgactgaaga ttataaagaa tggcttgttt ttcaagaaaa ctggcgcact gtacgcaatg
    84781 cctctttgat ggctttgaaa ttcccccatg atgagtatag gaaaggtcag cgtgaactag
    84841 cggtagctgc ttataaaacg atccgtacga aacagaaatt gtttgtagaa gcacctaccg
    84901 gaacaggaaa aacaatctct acgttattcc ccgctttgaa agctgtaggg gaagaagagg
    84961 gagaaaagat cttttatcta acggctaaaa caataactag acaagtagct gaagatgcta
    85021 tgacagcatt aaaagataca ggagctgaag taaaaagtgt cactttgacg gcgaaagaca
    85081 agatctgctt tttagatgag acgacctgta atccagatca gtgcccatat gccaatggtt
    85141 attacaatcg gattaatgaa ggtttatggg atcttttgaa tcatgagaat cagatcactc
    85201 gtgaagtcat cgaaacttat gcaagaaagc atacactttg tcctttcgaa ctatcattag
    85261 atgtcagtat ctggtgtgac gtaatcattg gggattacaa ttatttattt gatccaactg
    85321 tttatttaag acgttttttt gaagatgaaa aaaacgagga ctatctgttt ttgattgacg
    85381 aagcacataa tttggtgaat cgttcccgag agatgtattc ggcagaacta tcttatgaaa
    85441 aaacgaaacg aagtaaggaa gctataccaa aggaattcaa aaaacttcat cgccgtttca
    85501 acaaactatt aaaagaattt gattcgatcc gagaaatagc aaaagaagat cattgggatt
    85561 atcatcatca aaaagcaccg gctgaatctt tagtaaaggc aggttatcaa ttaagcgaaa
    85621 aaattaaaga atggctggca gaatttcctg agcacccatc acaagaacaa ttgcttcctt
    85681 attattttga tttgcttcat tttttgaagg tcagtgaatt ttatgatgat cactacgaaa
    85741 caacagtcga aaaaacgtat cgcgatctga tagtaaaaga gttttgtatt gatccaagct
    85801 tgtttcttga gcagtcgttg gataaaggaa aaagcagtat tcttttttct gctagttttt
    85861 caccactctc ttattatcag gaaacactgg ggggacaaaa aagtctggct tacaaactgc
    85921 cgagtccttt cccagaagga aaccagcaag ttttcatcgc aaattaccta gagacgactt
    85981 atcgaaaaag ggaacacagt ctggcaaaat tagtagagac gattcatgct tttgctgatg
    86041 gaaaggtcgg gaattatttc gttttctttc cttcttatca atatttagat cttgcggtgg
    86101 aagcattcag acagcaatat cccgacagca atgtgctaat ccaagaaacg gacatgaatg
    86161 aggaagaacg cgaacgattt ttaacaaaat ttcaaacaaa tcccgctgaa acattgattg
    86221 gattttgcgt acttggcggg attttttcag aaggaatcga tttgagaggg gaccgattga
    86281 tcggcagtat gatcgtaggt gtaggtttgc cgcagatgaa tcatgagcag gaactgatca
    86341 aatcatatta tgatgagaag gaacaccttg gatttgctta cgcttatcag cttcctggaa
    86401 tgaacaaagt cttgcaagct ggtggtcgag tgattcgtgg catggcggat caaggaatcg
    86461 ttgtattggc agatcaacga ttttctagtt tttcttatcg acgtttattc ccacaacatt
    86521 ggcaaacagc aagagaagta cgaagtgttg gggaattgga agaaggaata cagcgttttt
    86581 ggttatcaaa agaagcagta aatcaaaaaa acgaggaatg acagacgtca gcctcgtttt
    86641 ttgattaacg atagttagtg aattgcaact cgattggtaa gtcaagttta cgtaataaag
    86701 caatcacagc ttgtagatca tcacggttct tacctgtcac tctaatctga tcttcttgga
    86761 tttgtgcttt tactttcagt ttttcatttt tgatagctgt agtgattttc ttcgcgtttt
    86821 cacgatcgat tccgttgacc aaatcggcag tttgtcgagc tttgccacct agagcgtgtt
    86881 ctgattcgga aaaatggata tttttgattg gcacatttct tttgatcagc ttaccaaata
    86941 atacatcttt gatctgttcg attttgaaat catcatcgct caatacaact agattgctgt
    87001 tctctaattt gatttctact gtagaatcct tgaagtcaaa gcgattggac aattcttttg
    87061 ttgtttgctg aatcgcattt tttacttctt ctgtattgac ttctgataca atgtcgaagc
    87121 tagcatcttt tgccatagga accctcctga tatacatata aatatttacc atacatctat
    87181 cttatcatag gattagggca gattcttacc tgtttgctaa aattcttctc atttgagttc
    87241 gaagtatttt attttaatag ataaactgtt attcttacat ttggaatacc aaagtagggg
    87301 agaggtgctt gtgagtaaaa ttatgactgc gtatcataaa aatgaaagat taaagaaaat
    87361 agtggttatt ttgataaccg gattgaccgc tgcggtcgct ttaaattatt ttttgattcc
    87421 agccaatgtc ttttctgcag ggatgaatgg gatcgctcaa atcatttcct cgcttttata
    87481 tgaatggttt catatcaaga tagacacagg aacatttatt tttcttttga atatccctgt
    87541 gtttgtatta gggtttctca aagtaggaaa acgagcaacg atccttagtt ttgtgaatgt
    87601 tgtatgtgta tcggtgatga cgatgctgct tccagtcgga cagatcactg ataatatttt
    87661 gatgaatgcc ctagtaggcg gtgttttatt aggtcttggc gtaggattat ctttaaaaat
    87721 gggctttaca actggtggga tggatatcat ctctcttgta ctttcacaaa caactggaaa
    87781 aacagtcgga aactacatgc tgattctaaa tggtatcatt gtagtcatcg caggcttctt
    87841 ctttaactgg gaaagtgcct tgtatacgat tatttccatt tatgcaatga ctcaagtaat
    87901 tgatgccatc cacacgagtc atcaaaaagt aacagcaatg atcgttaccg tcaagccaga
    87961 agaggtaaca gaagaactag caaaacggat ggtacgtgga atgacattac tgccgtctgt
    88021 cggtggctat tcaaaaaaac aagggaacat gatcatgatg gtcatcaccc gctacgaaat
    88081 gtatgatctt gatcagatcg ttcatgaaat cgacgacgat gcatttatca atattttacc
    88141 aactcattca gtatttggcc ggtttgcaaa tgaaaacgaa caaaaaatgt atcgaacaac
    88201 tggcgtattg ccagaaataa aaaatcgttc acaaaaaaaa tgaggaatca ggtttatttt
    88261 agaataaatt taatctatta catctaataa gaacattttt ataaatttaa acga
//
LOCUS       DACDIG010000004        74969 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.004, whole genome shotgun sequence.
ACCESSION   DACDIG010000004 DACDIG010000000
VERSION     DACDIG010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 74969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74969
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.004"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            186..1475
                     /locus_tag="IV247_000319"
     CDS             186..1475
                     /locus_tag="IV247_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP8776417.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(1514..2119)
                     /locus_tag="IV247_000320"
     CDS             complement(1514..2119)
                     /locus_tag="IV247_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAP8776418.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(2324..2788)
                     /locus_tag="IV247_000321"
     CDS             complement(2324..2788)
                     /locus_tag="IV247_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP8776419.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(2810..3130)
                     /locus_tag="IV247_000322"
     CDS             complement(2810..3130)
                     /locus_tag="IV247_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP8776420.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(3274..4353)
                     /gene="pepA"
                     /locus_tag="IV247_000323"
     CDS             complement(3274..4353)
                     /gene="pepA"
                     /locus_tag="IV247_000323"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAP8776421.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            4553..4903
                     /locus_tag="IV247_000324"
     CDS             4553..4903
                     /locus_tag="IV247_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP8776422.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            5126..6073
                     /locus_tag="IV247_000325"
     CDS             5126..6073
                     /locus_tag="IV247_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8776423.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(6130..7548)
                     /gene="pepV"
                     /locus_tag="IV247_000326"
     CDS             complement(6130..7548)
                     /gene="pepV"
                     /locus_tag="IV247_000326"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAP8776424.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            7697..8542
                     /locus_tag="IV247_000327"
     CDS             7697..8542
                     /locus_tag="IV247_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAP8776425.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            8546..8986
                     /locus_tag="IV247_000328"
     CDS             8546..8986
                     /locus_tag="IV247_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAP8776426.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            9036..9230
                     /locus_tag="IV247_000329"
     CDS             9036..9230
                     /locus_tag="IV247_000329"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8776427.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            9366..10052
                     /locus_tag="IV247_000330"
     CDS             9366..10052
                     /locus_tag="IV247_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8776428.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(10460..11149)
                     /locus_tag="IV247_000331"
     CDS             complement(10460..11149)
                     /locus_tag="IV247_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8776429.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(11124..13253)
                     /locus_tag="IV247_000332"
     CDS             complement(11124..13253)
                     /locus_tag="IV247_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAP8776430.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(13301..14638)
                     /locus_tag="IV247_000333"
     CDS             complement(13301..14638)
                     /locus_tag="IV247_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAP8776431.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(14890..15249)
                     /gene="rplT"
                     /locus_tag="IV247_000334"
     CDS             complement(14890..15249)
                     /gene="rplT"
                     /locus_tag="IV247_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAP8776432.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(15302..15502)
                     /gene="rpmI"
                     /locus_tag="IV247_000335"
     CDS             complement(15302..15502)
                     /gene="rpmI"
                     /locus_tag="IV247_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAP8776433.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(15574..16098)
                     /gene="infC"
                     /locus_tag="IV247_000336"
     CDS             complement(15574..16098)
                     /gene="infC"
                     /locus_tag="IV247_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAP8776434.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            16418..17050
                     /locus_tag="IV247_000337"
     CDS             16418..17050
                     /locus_tag="IV247_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP8776435.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(17052..17957)
                     /locus_tag="IV247_000338"
     CDS             complement(17052..17957)
                     /locus_tag="IV247_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP8776436.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(18042..18695)
                     /locus_tag="IV247_000339"
     CDS             complement(18042..18695)
                     /locus_tag="IV247_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP8776437.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVAI"
     gene            19017..19961
                     /gene="mvk"
                     /locus_tag="IV247_000340"
     CDS             19017..19961
                     /gene="mvk"
                     /locus_tag="IV247_000340"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAP8776438.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            19954..20931
                     /gene="mvaD"
                     /locus_tag="IV247_000341"
     CDS             19954..20931
                     /gene="mvaD"
                     /locus_tag="IV247_000341"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAP8776439.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            20944..22029
                     /locus_tag="IV247_000342"
     CDS             20944..22029
                     /locus_tag="IV247_000342"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAP8776440.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            22026..23090
                     /locus_tag="IV247_000343"
     CDS             22026..23090
                     /locus_tag="IV247_000343"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAP8776441.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(23339..24142)
                     /locus_tag="IV247_000344"
     CDS             complement(23339..24142)
                     /locus_tag="IV247_000344"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAP8776442.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(24162..25004)
                     /gene="kduI"
                     /locus_tag="IV247_000345"
     CDS             complement(24162..25004)
                     /gene="kduI"
                     /locus_tag="IV247_000345"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAP8776443.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(25020..26027)
                     /locus_tag="IV247_000346"
     CDS             complement(25020..26027)
                     /locus_tag="IV247_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP8776444.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(26034..26678)
                     /locus_tag="IV247_000347"
     CDS             complement(26034..26678)
                     /locus_tag="IV247_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP8776445.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(26778..27536)
                     /locus_tag="IV247_000348"
     CDS             complement(26778..27536)
                     /locus_tag="IV247_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAP8776446.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(27663..28814)
                     /gene="fucO"
                     /locus_tag="IV247_000349"
     CDS             complement(27663..28814)
                     /gene="fucO"
                     /locus_tag="IV247_000349"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAP8776447.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(28896..29207)
                     /gene="rhaM"
                     /locus_tag="IV247_000350"
     CDS             complement(28896..29207)
                     /gene="rhaM"
                     /locus_tag="IV247_000350"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAP8776448.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(29209..30054)
                     /gene="rhaD"
                     /locus_tag="IV247_000351"
     CDS             complement(29209..30054)
                     /gene="rhaD"
                     /locus_tag="IV247_000351"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAP8776449.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(30068..31324)
                     /gene="rhaA"
                     /locus_tag="IV247_000352"
     CDS             complement(30068..31324)
                     /gene="rhaA"
                     /locus_tag="IV247_000352"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAP8776450.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(31321..32781)
                     /gene="rhaB"
                     /locus_tag="IV247_000353"
     CDS             complement(31321..32781)
                     /gene="rhaB"
                     /locus_tag="IV247_000353"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAP8776451.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            32934..33803
                     /locus_tag="IV247_000354"
     CDS             32934..33803
                     /locus_tag="IV247_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8776452.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(33929..34828)
                     /locus_tag="IV247_000355"
     CDS             complement(33929..34828)
                     /locus_tag="IV247_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8776453.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(34993..35817)
                     /locus_tag="IV247_000356"
     CDS             complement(34993..35817)
                     /locus_tag="IV247_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP8776454.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(35828..37123)
                     /locus_tag="IV247_000357"
     CDS             complement(35828..37123)
                     /locus_tag="IV247_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HAP8776455.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(37139..37618)
                     /locus_tag="IV247_000358"
     CDS             complement(37139..37618)
                     /locus_tag="IV247_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAP8776456.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(37624..38652)
                     /locus_tag="IV247_000359"
     CDS             complement(37624..38652)
                     /locus_tag="IV247_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAP8776457.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(38669..39775)
                     /locus_tag="IV247_000360"
     CDS             complement(38669..39775)
                     /locus_tag="IV247_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP8776458.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(40004..42601)
                     /gene="adhE"
                     /locus_tag="IV247_000361"
                     /gene_synonym="adhC"
     CDS             complement(40004..42601)
                     /gene="adhE"
                     /locus_tag="IV247_000361"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAP8776459.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(42906..43286)
                     /gene="yajC"
                     /locus_tag="IV247_000362"
     CDS             complement(42906..43286)
                     /gene="yajC"
                     /locus_tag="IV247_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAP8776460.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(43383..44528)
                     /gene="tgt"
                     /locus_tag="IV247_000363"
     CDS             complement(43383..44528)
                     /gene="tgt"
                     /locus_tag="IV247_000363"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAP8776461.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            44746..45444
                     /locus_tag="IV247_000364"
     CDS             44746..45444
                     /locus_tag="IV247_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP8776462.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(45612..48251)
                     /locus_tag="IV247_000365"
     CDS             complement(45612..48251)
                     /locus_tag="IV247_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP8776463.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(48870..49901)
                     /gene="queA"
                     /locus_tag="IV247_000366"
     CDS             complement(48870..49901)
                     /gene="queA"
                     /locus_tag="IV247_000366"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAP8776464.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            50094..51269
                     /locus_tag="IV247_000367"
     CDS             50094..51269
                     /locus_tag="IV247_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8776465.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            51273..51914
                     /locus_tag="IV247_000368"
     CDS             51273..51914
                     /locus_tag="IV247_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8776466.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            51911..52828
                     /locus_tag="IV247_000369"
     CDS             51911..52828
                     /locus_tag="IV247_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8776467.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            52825..53496
                     /locus_tag="IV247_000370"
     CDS             52825..53496
                     /locus_tag="IV247_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8776468.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(54037..54525)
                     /locus_tag="IV247_000371"
     CDS             complement(54037..54525)
                     /locus_tag="IV247_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAP8776469.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(54726..54992)
                     /locus_tag="IV247_000372"
     CDS             complement(54726..54992)
                     /locus_tag="IV247_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP8776470.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(54992..55255)
                     /locus_tag="IV247_000373"
     CDS             complement(54992..55255)
                     /locus_tag="IV247_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP8776471.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(55248..55520)
                     /locus_tag="IV247_000374"
     CDS             complement(55248..55520)
                     /locus_tag="IV247_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8776472.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            55731..55916
                     /locus_tag="IV247_000375"
     CDS             55731..55916
                     /locus_tag="IV247_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8776473.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(56058..57881)
                     /locus_tag="IV247_000376"
     CDS             complement(56058..57881)
                     /locus_tag="IV247_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAP8776474.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(58479..61184)
                     /locus_tag="IV247_000377"
     CDS             complement(58479..61184)
                     /locus_tag="IV247_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAP8776475.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(61490..62437)
                     /locus_tag="IV247_000378"
     CDS             complement(61490..62437)
                     /locus_tag="IV247_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAP8776476.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLEASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            62627..62896
                     /gene="rpsN"
                     /locus_tag="IV247_000379"
     CDS             62627..62896
                     /gene="rpsN"
                     /locus_tag="IV247_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP8776477.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(63072..63434)
                     /locus_tag="IV247_000380"
     CDS             complement(63072..63434)
                     /locus_tag="IV247_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP8776478.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(63445..64566)
                     /locus_tag="IV247_000381"
     CDS             complement(63445..64566)
                     /locus_tag="IV247_000381"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAP8776479.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(64582..64932)
                     /locus_tag="IV247_000382"
     CDS             complement(64582..64932)
                     /locus_tag="IV247_000382"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAP8776480.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(65062..66573)
                     /locus_tag="IV247_000383"
     CDS             complement(65062..66573)
                     /locus_tag="IV247_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP8776481.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(66895..68247)
                     /locus_tag="IV247_000384"
     CDS             complement(66895..68247)
                     /locus_tag="IV247_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAP8776482.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(68400..69476)
                     /locus_tag="IV247_000385"
     CDS             complement(68400..69476)
                     /locus_tag="IV247_000385"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAP8776483.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            69672..71417
                     /locus_tag="IV247_000386"
     CDS             69672..71417
                     /locus_tag="IV247_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAP8776484.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(71697..72134)
                     /locus_tag="IV247_000387"
     CDS             complement(71697..72134)
                     /locus_tag="IV247_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAP8776485.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(72118..73491)
                     /locus_tag="IV247_000388"
     CDS             complement(72118..73491)
                     /locus_tag="IV247_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8776486.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(73813..74301)
                     /locus_tag="IV247_000389"
     CDS             complement(73813..74301)
                     /locus_tag="IV247_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8776487.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(74323..74925)
                     /locus_tag="IV247_000390"
     CDS             complement(74323..74925)
                     /locus_tag="IV247_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP8776488.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        21514 a        16049 c        13767 g        23639 t
ORIGIN      
        1 tttccaatcc tatccgcgta gttttacaca gtatctgtgg aaaaagaaaa tcaaatattt
       61 tcatctgaaa acaatgattt ttcctaaagt ttttcataaa aagttcacag tctcaaagta
      121 gactaaagtc aacaaataag actatactaa cagtagataa atgaatgtaa ttggaggagg
      181 aacacatgga tagaaaaaat gtgacaccaa agatgaaaaa aagtaaaaat aatagcctct
      241 ggcgtaagtt aggtctgggg cttgttggag ggatcgttgg aggactactg acagctggca
      301 ttttttatgc agcgatgggc actggcaaca atagttctac tgctacaagc ggcaatcaga
      361 attctgccgg tgaaacagtt gtggaaaatg tcaaagtcaa tgtagattct gatatcacca
      421 aagcggtaga taaagttcaa gacgctgtcg tttctgttat caacttacaa agtcaaaacc
      481 aatcaagcgg ttttggcgga ctattcgggc agcaagaaga aagtagcagc tcagatgata
      541 gtaatttgga agcttatagt gaaggaagcg gcgtgatcta caaaaaagac gggaatactg
      601 cttatgtagt cacgaataat cacgtagtag atggccagca aggtcttgaa gtcttgatga
      661 aagacggtac gaaggtaaaa gctgaattag ttggaacaga tgcttattca gatttagcgg
      721 tgttgaagat caattcagac aaagtcgaaa cagttgcctc ttttggtgac tcaagtgctc
      781 tgaaggttgg tgagcctgcg attgcgattg gttccccact aggatctgaa tatgcaaatt
      841 ctgtcacttc aggaatcatt tcttccttga accgacaagt gacaagtacg aatgaatcga
      901 acgaaacagt caatatcaat gcgatccaaa cagatgcagc aatcaaccct ggaaattctg
      961 gtggtccttt agttaatatc gaaggacaag ttatcggtat caactcaagt aagatcgcaa
     1021 gtacttctga atctacttct aatgtaagcg tagaaggaat gggatttgct attccaagta
     1081 acgatgtagt gaatatcatc aatcaactgg aaaaagacgg aaaagtgaca cgtccagctc
     1141 taggtatcac aatggtcgac ttatctgccg tttctactca gcaacaagaa caaatcttga
     1201 agattccttc atctgtaact aacggtgtca tcgtgacaaa tgtccaaaca gctacaccgg
     1261 ctgaaaaagc tggattgaaa caatacgacg tcattactaa gatcgatgac aaagaagttt
     1321 cttctggtgt agaacttcaa tctgctttgt ataagaaaaa agtgggagat tctgtcaaaa
     1381 tcacttacta tgaaggctcg aaagaaaaaa caactactgt tgaattgacg atcgatcaat
     1441 cggcattaaa acaaaatcaa aatagtgaaa attaataaaa aagaggttgt gacaaagact
     1501 tgtcacaacc tctttatttt ggaaatgctt ctccgactac ggcatcctca ggcaatacta
     1561 agattccttt ttttgctgga gcatttggca aatgcaattc acgagcggaa cagatcattc
     1621 catagctatc tacccctcgc aaagttcctg gccagatcat cattccatct ggcatcatag
     1681 ctcctggttt tgcaacgaca actttttgtc cagcttcgat attgggtgca ccgcaaacga
     1741 tttgcaatac tgtaccattg tctacttctg tctcagtaat cgataaatgg tcagaatcag
     1801 gatgtgggac gcatgttttt acgtacccga caacgatctt cggttcatga tcagcttcaa
     1861 gctgttcttc gaatcctgct tctttgatca atcgattcaa tttcgctaat tcctcatctg
     1921 ttaagcttac ttggccgttc ccttcgattg ttagatgatc ggagatatgg aaaaagttcc
     1981 aagcaacaac ttgaccttgt tcatctttga tccgagcaac attccatttg cgttcaacag
     2041 tgttttctgt tcctttatcg tcggctataa taaccatcaa ggtgtctccg acgtgtgctt
     2101 tgttataagc aaaaatcatg ttctttcctc cattatcctt tgctgagatt tatcttaaca
     2161 aaaaattgca ttcactgcat gaaatttttt tcatgcttta tttccgaaga agcctcttct
     2221 ggaacaccat ttattcgtaa caagggactg tgacaagact tttgtcacag tccctttact
     2281 gttttagtta attgattaaa aaggtcgtac aagttccact tgcttaatta cttggctgtt
     2341 cttcttcgtc agaaacgatc aagacatcac aaggtgaatg acgtatcacg tagctggcta
     2401 cacttcctgt cataaagcgt tcgactgcat ttagacctga ctgcccaacc atgattagat
     2461 cgatctcata tttttttggc aatttgactg ccatggattc acgaacagaa ccatatgcaa
     2521 taattccttc tatatttgtg taatcaacag atttcccata ttctttacat tcttcgatca
     2581 tttccttggc tgtttcagtt tgctgatcga ctagattttg atcaataggt gcatagccca
     2641 taaagttata aaattgctga tcgattacgt ttgctacata tactttccca ttattacggc
     2701 gagccacttc taccgctttt ttgaaagcag caagtgcttg ttcactaccg tcgataccta
     2761 ctaaaatatt cttatattct tgattcatct tgacccctcc taacagatgt cagccttttg
     2821 ttacactttc taggaacgat tcgatttcct gttttgtttt acgatcttta ttcactagac
     2881 gacctaattc ttttccgtct tggatcacga caaaacttgg gataccaaaa atgttccatt
     2941 cagcggctac atctatgtac ttgtcacggt ctacttcaat aaattgccaa tcagaaaagt
     3001 cattttcaat ttcaggcatt tgcggtttga taaaacggca gtctccgcac cagccggcag
     3061 taaagaaaaa tacactcttt ccagtttcta cgtagcctgc taattcttct aaactatttg
     3121 ggataatcat tctttgtcct ctactttctg tttatgtatt tttcaattaa attatacacc
     3181 tttctttcga actaacaaac attgatcctc tttttataaa aaaaatctgg caaaaagcaa
     3241 tcttttgcca gattttatag tttcactatc gcattatttt ccgtatacga tcgtatttac
     3301 tgttgattta tccaaacctt tcagtgtttg aatcaacatt tctcgtgcag cttcaaaatc
     3361 gcgaatactg aacatcgttt gatgcgtatg gatataacgt cccactacgc caatcactgt
     3421 acttgggatt ccttcgtttt gtgtatgagc agcacctgca tctgtaccgc cttttgaaac
     3481 aaagtattga taagggatat tgtttgtttc agcgatatcc agcaagtact cgcgcaaacg
     3541 aggaagcatg atcaaacctg gatcttggat acgcatcaat gtaccttctc ctaaatgtcc
     3601 gaatgtgcca tttttcgtca ctgtatcatc tgctgcagag cagtctactg caaagaacag
     3661 atctggcttg aatttattga cagaagcttt agaaccacgc aagcctactt cttcctggac
     3721 attcgcgcct gcaatcaatg tatgacctaa tgtttcattt tgcaacgctt ctaaagcttc
     3781 taataccatt gtacagccat agcggttgtc ccatgattta ctgataatat tctttccgtt
     3841 ggctgttttg atcgtttcag cgtaaggaac gattgtgtct cctggaagga caccgtaact
     3901 cattgcctct tctcttgatt caaacccagc atcgaacaaa atatcggtta cttctactga
     3961 tttttgtccg cttgttcctc gcaataaatg tgggggaacg gaagaagaaa tacatggata
     4021 attcttacca gtagaggttt taagtgtaaa acgctgagca gaaacaacgt aaggattcca
     4081 gccgcctaga ggtacgactt taaaaagccc gttttcttgg atttgtgtca gcatgaatcc
     4141 gacttcatcc atatgtgctg cgaccattac acggggtgca tccgcttctt catgatggcg
     4201 cagaccaaaa atgccgccca aaccatctaa ctgtaactca tctaccagcg gttccatatg
     4261 actttccata taagcgcgaa catcttgttc aaaaccgctt gttccttgta attctgttaa
     4321 ctctttgatt cgttgaaatg ttttttcttc catatgctat tcgtctaaat ttcagactaa
     4381 actcctctca gacttatatt gatctgcgga taaaatatga ttttttctgc agaccgatcg
     4441 tatttattat agcttatttt ttctggtagc ggttatttat ttcaaagaaa atatgagagt
     4501 gtggtaaaat aagcacaagg agtaacaagt gagaagaagg aggtatgtcc acatgtatga
     4561 agaaagcgaa ctaagctatt ttaaaggcgg gttagcagtt ggaatcggtc taggattatt
     4621 gagcggggta gcttcaacga tttggtatca tcgcaaaaaa gtattgtcag cagaccaagt
     4681 acttgaaaat atcaagtcat cttttctaaa agaaggtgca atcgaaggtt cttggatctc
     4741 ttttgaaaag gaacctatta ggaaattcgc cgttcggtcg agtgcttatc ggggaggtct
     4801 ttctcgaata gaagatggcg aaactgttta ttatgaattt ttagcagatg ctcatacagg
     4861 aaccgtatta gaaatttcaa gaaaaaaagg tgcaacagat taatacgtca aaaaagtgag
     4921 agaacgaata cgcacccact attcaaaaga gaggtatcat gtccttcttt tgtagtgggt
     4981 ggttttttag atagagcttt ccaaacatat attccagtat tttcttttta aataaatgat
     5041 atttagcggg aaaaactagc atttcctttg attatgaggt atttttaatt tagcttttgt
     5101 tttctatagt taaggagagt gaaaaatgga acaacaagaa aaaattgctg ttctgattcc
     5161 ttgttacaat gaggaagcaa caatttcaac agtcattgca gactttaaac gagaattacc
     5221 agaagcagat atttatgtct atgataataa ttcgaccgat cgaacatacg aattggccgt
     5281 tcaaggtgga gcaatcgtaa aaaaagaacc tcgtcaagga aaaggaaatg tgatccgcca
     5341 aatgttcttt gatatcgatg ccgactatta tttaatggta gatggcgatg atacttatcc
     5401 tgcagaagct gtacatggtt tgctcgagaa attacgttct ggtgaagcag atatggtcat
     5461 tggtgatcgc ctatcaaatg gaacctactt tgatgagaat aagagacctt tccacgattt
     5521 tggaaataac cttgttagaa atacaattac tagaatgtat aaaacaaaaa ttcttgatgt
     5581 catgaccggc tatcgaggat tcaatcgtat ctttgtcaaa agttttccta ttatgagttc
     5641 tggttttcaa atcgaaacgg aattgacgat ccatgcttta gataaaaaat tcaagttcgt
     5701 tgaaatccca atcgattatc gtgatcgtcc tgaaggcagc gaatcaaaat tgaatacatt
     5761 ctctgacgga ttcaaagtga tcatgatgat tattaaaatg tttaaagatt ataagccttt
     5821 gcttttcttt gggatttgga ccattttctt ctttttattt ggattggtca cggggattcc
     5881 tgtgattcgc gaatatatgc tgacaagctt tattacacgc atcccttctg cgattttaag
     5941 tactggattg atgactttag cacttctttc attagtaaca gggttgattt tggatacagt
     6001 cgtcacgaac gctaagaaag aatatgaatt gaatctatat catgtttatc atgaatacca
     6061 cagtagaaag taaaaaaagg atcaagactg caaaaataat ttgcaattct tgatcctctt
     6121 tttaattaat tatttcgcca aacgatagat ggcatctgca tagattgctg ttgctctgaa
     6181 caagtcatca agactcataa attcgtttgc ttgatgcatt gtatcagtat atcctgggaa
     6241 catcgctcca taagcaacgc cacgtttcaa caaacgtccg taagttccgc caccgatgat
     6301 ttgttcgtag cctttttctc cagtgtggtc ttcatagact tgcaataatg tagccaccaa
     6361 tggatcatcc attggtacgt aatgtggttc cattacgcga gcacctcgag taactgttgc
     6421 gccttcttgc cccactgttt gttcgatgtc actttgcaat ccgtctactg ttacaccttt
     6481 tggaaaacgg aaattcatat tgatgaagtt cgcttcctct gtaccatttt cttcaaaagc
     6541 aaatagtcca gcattcattg tcaatttgcc cattttttca tcttcataag cgactcctag
     6601 tttttctcca taaaaatctt catggacata agcagctgct acatggataa attgtttagc
     6661 agatcctagg aattcatagt tatctaagaa caatgccaag aaagaacctg cattgatacc
     6721 agattgtggg cttgcaccat gtgcgccttt accaactaat tcgattttta catacgtatt
     6781 gtccgcttca atcgttccgc ttactggatt tgcttcgatg aattggtaaa atgcttcttc
     6841 catcgcgata gctgcatctg cacttggtac ttgaagtgct gcagttgctg ttcctggcac
     6901 catattttcg cgaagtccgg aaacaaaaga tttcaagaca taatctcctg cattgtcgcc
     6961 tttgaagcgt aaagctaaag aaacatttcc tttttcacca ttgatgattg ggaattcagc
     7021 atctggtgag aaaccaaaat ctggttcttc ttcatgttcg aaataataag ccatatcgcc
     7081 ccagccgctt tcttcatcgc tgccgactac gaagcgaact ttttttgata gtttcaagtc
     7141 aagttcttta atgatcttca tcgcatagta agctgccata cttggacctt tgtcatcgct
     7201 tgatccgcgt gcaaagatct ttccatcctt gattactggt tcataaggat ctgtgtccca
     7261 gccatctcct gcaggaacga catccatgtg tccaaatatc cctagcgttt cgtctccttc
     7321 gccaagatca atatgccctg catagttatc gacatttttt actttaaatc catcacgttc
     7381 gccataagct aacatatgtt tcaatgcgtc gcgaggtcca ggtccaaatg gtgcatctgg
     7441 tgttactttt gagtcatcgc gctcgctgtt gacacgtaaa agatttttca agtcttcaag
     7501 caagtcttct ttacgtgctt ctacttcttt ttgccaatca attgtcataa agttaatcct
     7561 ccacttattt aagtcttcgt gttcatgata ccacaaatta cagaaaaaaa tagttcaaaa
     7621 aatctgacaa ttccttttct tatctgtttt tctctaaaaa aaatatacgc tataatgagt
     7681 aaaaggaggt ttttagatgc tgataaacga acaactagtg aaaagagtca tcacacctcg
     7741 tccaaaaatt tcacataaag gaacatttgg acgtatcgtt ttgattggcg gcaatgaaca
     7801 atatggcgga gcaatcatta tgagtgcttt agctgccgta cacagcggag cgggtctcac
     7861 gactgtagta actgatgaaa aaaatcggag tgctcttcac agtcatctgc cagaagcaat
     7921 gattgtagtt tggacggata ctgaccagtt tctttccgta ataaagcaag cagatgtact
     7981 cttgattggt cccggattgg gtacttcttc taagagtttg gaacgtttca aacaagtact
     8041 agaaaaccaa tcaaaagaac aatggctggt gattgatggc tctgctttaa gtttacttgc
     8101 tgctaatcat cttttgctac cctatcccga acaaactgta cttacacctc atcaaatgga
     8161 gtggcaacgg ttgagttctc taccgataca agaacaacgg gataaagaaa acaaaaagaa
     8221 gcaacaggaa atcggtgctg tgctcgtttt gaaaagtcat gagacaaaaa tttatggtca
     8281 ccagcacttc tacaccaatc ctttaggttc tgctgctatg gcaacgggcg gcatgggtga
     8341 cactttagct gggatgatca caggattttt agcccagttt cctcaaaatg accaaacgat
     8401 tgcagcagct atttatcttc atagtttcat tggagaccag ctggcaaaag aacaatatat
     8461 tgttttgccg acacaaatca gcgaagcact tccgaaatgg atgaattatt tttgtaaaaa
     8521 atcaatggaa aacgaggcat gagtcatgga gaaatttaca ggttatgtgc gagaaccttt
     8581 ttattatgaa acagatcaaa tggggattat tcatcacagc aattatatcc gctggtttga
     8641 agaggctcgg gtagccttat tggtacatct agggtttccc tataaaaaaa tcgaagaatc
     8701 tgggatcatc atccccgttc tggaagtaaa ttgcagctat aaagaaatga tccattacgg
     8761 agaaaaagtg agcatccatc caactatcca aaagtatacg gggacacggt tggattttag
     8821 ctacgaaatt gtgggaactg aagacaaaaa attaaaaact actggcagta gtaaacactg
     8881 ttttttactt tctgattcgc aacgattagt gaagctgagc aaagtcaatc cggagttaga
     8941 tcagttattc caagcgtacg cttctttgga tgatgtaaat tcttaataaa gaccgtctaa
     9001 gcatttttct tgtattgtgt cgagtagttc gtataatgaa aagaaaaaag caggtgatcc
     9061 ccatgtatgt cgttaaagta atgcacggtt acatcgataa aactggctgt cgcactcggg
     9121 aaaaaaactt agacaatctg ctcattttca aagacaaaaa agaatccgaa gcttttgcta
     9181 aaagaatcgg cggtcgtgtc aaaccgatcc aagaggtacg accagattga ttcaatattt
     9241 ttcttttcga ggctctaata ggcatgccta tcagggcttt ttttgcagac tgtgttttta
     9301 tgcccacacg atttgtaagc gatttatgtt ataatagatt atatctgcat agaaaggaag
     9361 gttaaatggt tatcaataat ttgcaacagc aagcttatga atctatacgt aaacaaatca
     9421 tttattccga gttagcacct ggaagtaaaa tctccgataa agtgttagaa gaacaactag
     9481 ctatcgggcg tacaccgatt cgagaagcac tgatccaact gcgcaatcaa gagcttgtaa
     9541 aaaccattcc acagtccggc acttatgttt caaaaatcga tatccgatct gcttcattag
     9601 cccgttatac gcgggaaaag ttggaaaaac cgatcctgca agaatgcagt gcgaaaatga
     9661 ccaagcaaga tcaagctaaa ctagaaaaaa tactagaaca aaccgatcag gcagtaatcg
     9721 ctcaaaataa aaaagctttt tttgatttgg atactgattt tcaccgaact tgttatgaaa
     9781 ttgctggaaa acgagaaatt tgggattggc tagaaagtta tagtacgcac ttgaaccgtt
     9841 tcagatggtt acgtttgacg atttcagaat tagactgggg tcgagtatta gatgaacatc
     9901 agacaatgct tcaatcaatg attgatcata actttgatga agtcggattt ctttgcacga
     9961 tgcatttaca tatgattatc gaggagcaag aatacgtgat tcataattac cccgattatt
    10021 ttgaagatat tcaggacaga cccatcaaat aagaggtttt ggaatcagcg ttttgacctg
    10081 agtattagag aaagaaacgg caaaatagtt cctcatattt tgccgttttt tttgagctaa
    10141 taccgagggt cagctgattg cacaccgttt attgagaggt tgtgaaaaag ctgccctgca
    10201 tcaagtaata agaacagcca agtaaaaata gcttcttatg tttttaactt ggctggactt
    10261 attaccgcag atgcaagctt ttgaacaccg ttttccaaga ggtcgtgagc ctgccgttca
    10321 tctccgagtc acaaggaaca atttcaaaaa acagcttctc atgtttgttg aatattttga
    10381 cttgtgaccg aggagttgac aggagaacat cgtttattcg gaaaaaaagg actgtgacac
    10441 gacttttgtc acagtctctt tatttgtaat ctaaatcata ctccgtacat cttttttcta
    10501 ttttttttat ttcttctaag atcccttggc tacgaatttc attatatggc caaagtaaga
    10561 ctgcctctaa tacataaggc gaaaaatcgc tttgattcat ctcttttgaa aaactcatcg
    10621 ctgctatctc gctcaatgca cgaatgtttg attgatgtgt ataaaaaggc aaccgccaca
    10681 gcacaatttt ttcgctttgc gtataaattc ccttaacaga agcttcgatg tggtgaaata
    10741 gttcatgtcc aataatcaaa gcttgcgctg tttctttaga gatcgaagct agtcctgaac
    10801 attttaattt ttctaacggc tccgtcatca atcgaatccc ttcatcttct gcataagttg
    10861 caaaagtgat tcgttcgttt atttgctctg attcacagga aacgattgga acagccattt
    10921 gttcacaaat ttctcctaca tcggcaaaag ggtacttttt tttgatgcta catgccgttt
    10981 cgataccaca atcgattgat tgttgtataa gaattttctg cttggacaaa ggaatctttt
    11041 tccaaaataa atctcgccga aactggtaga atccccaaat ttcttcgggg atctgttgta
    11101 attcgtttag taactcttct aacttatgtt tgtttgatat ttccggcaac gacgatcact
    11161 tcttcctccg gttccatagt agatacttcc agatccatca aaagctgtag ttgttccaga
    11221 tccgtcgctg taaaatcaga gactcttgct cctaagcaga gataaaattc tggtcgggca
    11281 atcaattcag tttgataaac tgccatatct tcccaaagtt gttcgacagc tgtcatataa
    11341 ttggctgcag ttgttttcat acatgaagca tgtccacgtt ggaccttgat aaatcctttt
    11401 tgatcgatga tccgaagact tccagcattt tttgaacgat tctgttcgcc gcatacataa
    11461 aagaaaggtg tattttccaa taagcaaact tcatttttat ttaatcgcat atcctctgca
    11521 gcaagctcta gtgcttcttc tgttgtgatc tccttggtta gatcggttgc tttcacttcc
    11581 gtagaaccag tagcgatagc cgttactttt gaagtctgtg gatcgatttc tacgtggact
    11641 tcgatcgatt ctggtgtagc gcctgattca atcgctttat tcatcgcttc atttttcaac
    11701 gatcgtatat cttctttact tggcgaaggg atgatacgtt ctacaacatc tcggaccatt
    11761 gcaagtgcta caccaataga agaaatcact tcggcgtttt caggaatcga atatttcaca
    11821 cccattttat tactaaagta agtaatcaat gaggcagcgc cgccccctac acctacaaga
    11881 gaaatctgat ctttctccaa gtgatacttg tctgccaatt ctaaaatcac cggttcaatc
    11941 ttagcatagg atttttccat gatctgttct gcgatgtctt caaccgtcgt atgacaataa
    12001 tcagctaatg cttggatggc ttttcgtgca gatgggacat ttccataaga aaaatgttct
    12061 tcttgcacta atccaaggac attggctgca catgtatttg taatagtcac ttcttctccg
    12121 tcttccatca ctaccttgac gtaatcagca gggtctcctt ctttaggaga gaaaaattca
    12181 accttaggac ctttgatttt ttctgtctcc gtaaatactg cataatctag accggcaata
    12241 tgtgcagaac gtggaccgac atcgatgatt cctgactgat tagcccgtac catcgaaccg
    12301 cctgccacac ctagaacccg aacatctaat gaagagatat atgttgggtg gccacctaca
    12361 attgaataat cgattgctgg acgtccattt ttaatgaccc cgatattggt tgttgttccg
    12421 cctacttcaa aataaacacc attagacgct ctcaagtaca tcaaagagcc catgactgat
    12481 gctgctggac cggaaagcat ggttaaaaca ggtcgttttt tcatttcatt gatttccatt
    12541 actccgccgt ctccgcgcat aatcatcaaa gaaacattga cccctgcttc tcgtacggaa
    12601 tcttctgtag aggttgccgt atctagcatt tttggtaaaa tagatgcatt gattgctgct
    12661 gtccgagttc ttcttgtcaa tccatataat tttgtgatgt cagaagccat cgttgtaggg
    12721 atcgatttta ccgatgctgc ttcataaaca actcgttctg gttccccatt atctactcca
    12781 aatgccatag aagaaaccaa gacctccgct ctttctcttt ctaaagaaga aatcgtttct
    12841 gaaacacggt ctacatttaa gtgtttgaca ggtaagaatg cattgacaat ctctattttt
    12901 ttcttattcc ctaaatcgat atcgttcaat ctagtttgtc gttttgctaa aaaaccttct
    12961 aatccacctt ttgccatccc gattacgccg acttttgcta cgtctccttc aatcagtgca
    13021 ttagtcgctt gtgtggtaga atgtgcaaca aacacaacat cttctggact gatattattt
    13081 tccagcaaac aattttgaaa agactgaacg acacctgcgg ctactccacg gacatcatcg
    13141 tgggttgttt tcactgaaga ttttccgatg atttcgtgcg tcgcattatc gattgcgact
    13201 gctttcgtat gtgtaccgcc aacatcaatt cccattcgca caaaacgttt cattttcttt
    13261 tcctccattc atctaatcgt ttttatcgac gttttgttta ctaggcgttc ccgtacaata
    13321 agaacgtgat caggcataag atcgctccga tgatccaacc agttgggatg gatttcttca
    13381 tatattctcg agaagttact tttgtataac ctaatcccca agcaacccaa gattgtgtaa
    13441 tatctaaatg ctgaggagca atcgttgtga ttgcaaataa cgggaacaaa aaggctggcg
    13501 acatcgttgg attaacagct aatacgacag ctaaaatagc tgagcctgaa ccgactagat
    13561 tcattggacc gcggaaaaat cctagtggtg tcaatatagc aaatacgatt gccaagacta
    13621 atggtgattg cggtatccaa tctccaaaaa tagctgaaaa ataaggagaa gcatacgcag
    13681 ctgaattatt gaacatcgct aaggtcatca agaaaccaac cattggcgca acatctactg
    13741 cgccatctgt aaataatttc gcaaactgac gacagatttc tttataacct ccgcgcaatt
    13801 ttccagtaaa cagcaatgcc cagatacctg ctagaataaa acctaagatg attggaagat
    13861 ctaaaagaac gacccctaaa actggtaata atacggctaa ccatgagatc attggtgcgt
    13921 cgccaccttc agctggtacg tttgctgcca ttgcatagcg tttctttttg ttcattgata
    13981 tattagcgac cgttagtacc acaactaagc ttacaatcat cccaatcata ccaatttgga
    14041 aatagtcatt ataagtatag ctttcagccg ctggattgaa accagcataa atcgtttgat
    14101 attgtttgaa attgacgata ttagcaaaga ttccagccat aatcgagccc ataaatgaga
    14161 acagtgcaac cggtactgaa atacctaatg acatcaagat tggcaacaca ataactgcaa
    14221 tagaaatcac tggtccgatt cctgtcatcg acatgaatat aacagcggtt acaatacata
    14281 gtaagctcat cgtgattctt ggcttgtccc ctccaagttc aactaccttt cgaatcaggg
    14341 tagctgcgat tccgctatct actagaacac gcccgaaaaa ggcaccaaag aagacattta
    14401 ccaagatcga tttggcgtac tcagctggcc ccgtcgtata gatatgcgtc aacacatcaa
    14461 ttactccttg cccttccatc gccgggtttg gtgaaaacgt gtttccgact agcgctaatc
    14521 ccatccatac agtagccatg atggcaaagc cgaccatcaa gttgtagcct tttacacagt
    14581 accagaccat gaaaaagaat gagataacta acaaaatacc aataataata tcactcatgt
    14641 tattcctccg tccattttgt ttttctgata agcaaactaa tatatcagtc aaactcattc
    14701 taacttatgt agttagcgct ttcattaaag ggtgaccata aatgttcagt tttcaattaa
    14761 aaaaccttaa ttatgctatt ttagacttat acgttattgt cctgtttcat ttgatggagg
    14821 attgtaaata aaaaaagagg aaagaacata acttttcgtt atcttctttc ctctttataa
    14881 ttgtttagct tatttagcca aagcttcttt tgcttggtca gccaatgcag taaatgctgc
    14941 tgcatcgtta acagccaagt cagctaacat tttacggttg atgtcgattt cagcaacttt
    15001 caaaccgtgc attaattttg agtagctcaa gccattcata cgagctgctg cgttgatacg
    15061 agcaatccac aatttacgga agtcgcgttt cttttgacga cgatcgcgat atgcgtagtt
    15121 gtatgaattc attacttgtt cttttgctgt tttaaataaa gtgtgttttg aaccgtagta
    15181 accttttgct aattttagca ctcttttacg acgtttacga gttactgttc cacctttaac
    15241 acgtgccatg tataattcct cctaataaaa ataaatcttc agattctgtt atttgtactt
    15301 cttatttcat ttttgctagt tgttggcgaa tacgtttgaa gtcgccacta gaaaccatgc
    15361 ttgctttacg caattgacgg cgttgttttt ttgttttgcc gtggaaacgg tgacttgtaa
    15421 acgcgcggaa gcgtttcaat ccgcctttac cagtacgttt gaaacgtttt gctgatccgc
    15481 ggtgtgtttt ttgttttggc atgactatat ttcctcctca aaatctttcg ttatccgact
    15541 atcggctgtc caacagccag ttagtcattc aaattactta ctgtcgtttt tcggtgccag
    15601 cgtcaagaac atgctgcgtc cgtccatttt cgctttttgt tccactgttg caatatccgc
    15661 agtttcttca gctaagcgat tcaagacttt ttgaccaatt tctttatggg taatggcacg
    15721 gcctttgaaa cggatagaag ctttcacttt atcgcccttt tccaagaact tacgtgcatt
    15781 acgaagtttt gtattgaagt cattcaagtc gatcgtagga cttaagcgaa cttcttttac
    15841 attgatcact ttttgttttt tacgcgcttc gcggtctttc ttttgttgtt cgaaacggta
    15901 tttaccgtag tccatgattc gcgcaactgg tggtttcgca ccaggggcaa ctaacacaag
    15961 atccaagttt gcttgttctg caatattcaa tgcttctgct tttgtcttaa ctcctaattg
    16021 ctcaccatct gaaccgatca atcgtaactc tcgtgcacga atgccgtcgt taaccatcat
    16081 atcctttgct atggtcattc acctccaaat attatgagag aaagaaaagc ttaccgaata
    16141 aacggcaata aaaaaacgag ttctcttaca gaacccgcgc gtacttcacc taatagaacc
    16201 agtgaaaact atctagccca gtgacattat caactaaggc gagaagcggg tgcttctgct
    16261 tttatttctc aaccatcata gtatagcaaa aactaatctt tttgtcaatg tttttcatac
    16321 agtttgtctt ccttttctga aaatgattag attagcagac tttctagcag tgattcccgc
    16381 ttctttgcta tactaaagaa aaacgaggtg aacccacatg aaaaaaatcg aacctattcc
    16441 accttctctt cctcttctta caaagggaga gttttattta gaagatgagg agatcttctc
    16501 tggtcttctg atcaacgggg gtgaccaaag ctatctcgat gccaaaaata tggtattaag
    16561 agagagtcat atccaaaaac ttacgatgca aaaaacagtt ttacgtcatt ttgaatgcag
    16621 taatgtcatt tttgaaaaat gtgatttttc caatctggag tggattggtg gcagtttcca
    16681 ccaagtcgtg tttcatcaat gtaaattaac aggtacgaat tttgccgaaa gttatcttcg
    16741 agactgtact ttcactgatt gtattgcttc gatggcttcc tttagtagta caaacttgaa
    16801 agctgtctcc ttcgatcatt gtcaattaga agacagcgaa ttttatgagg tcacttggaa
    16861 aaaccttttt ttgtctgaca atcagttgac cggttcgaac tggtttcgaa cttctctgaa
    16921 gggattagac tttacgacta atttcttcac agcaatcgct ttatctcaag attctttggc
    16981 gggactaatc gtcaaccaag agcaagcact tgtgatcgcc caagcccttg gtttgcagat
    17041 caaagactag atcaacgaat ccgccgccac agtgcaataa gcatccaaat agataagacc
    17101 aaactgatga tcaaggcgat tccccatccg aaaacggaat gggtaaaggg taactggaga
    17161 ttcattccga agaatcctgt cacaatagtt ggaacggtca agatcagcga ccagaccgtc
    17221 aaaaatttca tcgtgtcatt caagttgtta ttcagcaaat tattataagt gccagagagc
    17281 tggtctaaaa tctgcgaagc taagttcgtc atttcaactg cttgtcgtgc ttcgatcagc
    17341 gcatcgtcca actcttcttt ttccttttct gacaacttgc gataaatagc caatgccttg
    17401 atttgttcta aaagtactac attttgtttt gttcctgtaa ctaagtaaac caaaccgatc
    17461 tctaaatctg aaagttgcag cagattcttg ttcgttgttt tttctcttaa tttcagatta
    17521 agccgctgac gttcactatt cacttcctcg attaaaggaa aaaaggaatc agaaatcaaa
    17581 aacaatgtac gaaacaataa actgtataca ctcatgtctg gcattcgttc caaaagcgat
    17641 cgaattagtc gaatcacgta ctcggtattc ggcgtggtaa atgtaaacaa accgtcttct
    17701 tttaaaataa aagcgatagg actagtttca taatgattat cgactttttg ctggtgcgga
    17761 acatttgata caagcagtaa cgcttcatca aaagaatcgt actctacgcg cgcccgttca
    17821 tttttatcta gagaataagt cagcatctcg tcactaatgc catattcttc ttttaagttt
    17881 ttaacagcta gcacatcgtt ttgctgtaca ttgatccaaa aattacggtc ttgattaaaa
    17941 tagtgttttt cccgcatact ctatcctcct gacatttgtt tccagtataa agaaaaaagt
    18001 cgctcaaata aaggtttacc ctttatttga gcaactgtaa atcaaatggc tactttggtt
    18061 tggcgaatct ctgctaattt ttcttcgatc attttcaaga cttcttcttt tgcttgagga
    18121 tcttccacaa aattatagcg gtctccatcg atttggattt ttggactttc tttgtattct
    18181 tcataccatt gatcgtaacg tctgttcaat tctttgtaat aatcgtatag acttggatca
    18241 aaggacaatt gttcatacga acgaccacgt ttttcgatac gctccaacat cgtatcaaaa
    18301 gatacacgaa tatgaactaa taaatccgga tgttttttat gggcagcata gggaagttct
    18361 tccatcatgt tttccagcag ttcatcatac acacgaactt ctgtttctgt tgctcttcct
    18421 agatctgcat ttagatggaa aagcagtgaa tcttcataaa tcgaacggtc caatacatta
    18481 ttgtcatctt tcattgcttg tttgatgctt gcaaaacgtt tattcaaaaa ataaatttgc
    18541 agcaaaaaag catactgttc agggtttgca taaaataagg gcaagacttc attatcatca
    18601 atcgattcat aaaatgcctg actatcaaag tgttcagcca tcatttctgt taaacttgat
    18661 tttccggcac caattgtacc tgctaaaacg atcacgctca tttacctcca gctcacttca
    18721 tcactaaata ttgtgtttaa ctccaacctt tttaaatata cccccaatat ctagtagtgt
    18781 caattgtatt tttttctagt caaagaagaa aaaaagtcaa aaaattctta agaaaaaagt
    18841 gttttttctt tagaaaacgt attcttagtt ttacttctga gatggtttta gtaaaataaa
    18901 ggaggaatgt tttttgagac ccttagtgga caaaaaaatt cgtgaagcag cgcaactcgt
    18961 tttgggcgct ttattttttg cttaaaaatc acttttacgt gagaaggagt tagattatgg
    19021 caaactatgg ccaaggagaa tcaagcggaa agatcatatt gatgggcgag cacgcggttg
    19081 tttatggaga accagcgatt gcctttcctt tctatgcaac aaaagtcacc gcattccttg
    19141 aagagctgga tgcaatggac gatcaactgg tttcttccta ctattcagga aatttagccg
    19201 aagctcctca tgcattaaaa aatatcaaaa aattattcat tcacttaaaa aaacagcatg
    19261 acatccaaaa aaacttgcaa ctgaccattg aaagcacgat tcctgctgaa cgtggaatgg
    19321 gatcaagcgc tgcagtcgcc acagcagtca ctcgtgcttt ttatgattac ttagcatttc
    19381 ctttgtctcg tgaaatacta ttagaaaatg tccagctttc ggaaaaaatc gcccatggta
    19441 atcctagtgg aatcgatgca gccgctacta gcagcttgca gccgatttat tttacaaaag
    19501 ggcatccttt cgactacttt tctttgaaca tcgatgcttt tttgattgtc gctgatacag
    19561 gaatcaaagg acaaacaaga gaagccgtca aagatgtcgc tcacctcttt gaaacccagc
    19621 ctcatgaaac tggacaaatg atccaaaaat taggatactt gacgaagcaa gcaaaacaag
    19681 caatcattga aaattcacca gaaacgttgg cacagacaat ggatgaatca caatcacttc
    19741 tggaaaagct gacaatcagc aatgattttc ttaatctatt gatccaaaca gcaaaagata
    19801 ccggggcatt gggtgccaaa ttaactggcg gtggacgcgg tggctgtatg attgcattag
    19861 cacaaacaaa aacaaaagcc caagaaatca gtcaagcact tgaagatgcg ggtgctgctg
    19921 aaacttggat acaaggatta ggagtacata cctatgttta aaggcaaagc acgcgcatat
    19981 accaacattg ctctaatcaa atattggggt aagaaaaatg aagaactcat ccttccaatg
    20041 aacaacagcc tttcgttaac attggatgca ttttatacag agacagaagt catcttttct
    20101 gatagctata tggtagatga attttatcta gatggcacct tgcaagacga aaaagcaaca
    20161 aaaaaagtca gtcagtttct tgaccttttt cgtaaagaag ccggcctctc actaaaagct
    20221 tcagtaatca gtcagaattt cgttcctacc gcagctggat tagcctcctc tgccagcggg
    20281 ctagctgctt tagcaggcgc ttgcaatact gctcttaagc ttggattaga tgatctctct
    20341 ctttcaagat ttgctcgacg cgggtctggt tcagcttgcc gaagtatttt cggtggtttc
    20401 gtcgaatggg aaaaaggcca tgacgactta agttcttacg ctaagccagt cccttccgat
    20461 tctttcgaag acgatttagc aatggttttc gttttgatca acgaccagaa aaaagaagtg
    20521 tccagcagaa atgggatgcg tcggacagtc gaaacatcca gtttttatca aggctggtta
    20581 gattccgttg aaggggatct atatcaattg aaacaagcaa tcaaaacaaa agatttccaa
    20641 cttctcggag aaacgatgga aagaaacgga ctaaaaatgc atggaacgac tttagctgcc
    20701 cagcctccat tcacttactg gtctcctgac agcttaaaag cgatggatgc ggtgcgacaa
    20761 ttgagaaagc aaggcattcc atgctacttt acgatggatg ctggccctaa tgtcaaagtt
    20821 ttagtggaaa acagccattt atctgaggta caagaaacat ttaccaaatt atttagcaaa
    20881 gaacaagtaa ttactgcaca tgctggtcca ggaattgcga ttattgaata aagggagatt
    20941 tttatgattg aagtatctgc accaggcaaa ctctatattg ccggagaata tgcagttgtt
    21001 gaaacaggcc atccagcagt tatcgctgca gtcgatcaat tcgtgacagt cactgtagaa
    21061 tctgcacgaa aagtcggaag tatccaatct gcacaatata gtgggatgcc tgtacgttgg
    21121 acaagacgca acggagaatt agttttagat attcgggaaa atccttttca ttatatcctt
    21181 gctgctattc gcttgactga aaagtatgca caagaaaaaa acattctttt atccttttat
    21241 gatctgaaag taacaagtga gttagacagt tcaaatggcc ggaaatatgg attgggttct
    21301 agcggtgccg tgaccgttgc aacggtcaaa gccttgaatg ttttttacgc gttgaattta
    21361 tctcagttgg agattttcaa gattgcagca ctagccaatt tagcagttca agataatggt
    21421 tcctgcggcg acatcgctgc tagctgttat ggtggctgga tcgctttctc aaccttcgac
    21481 catccttggc tccaagaaca agaaactcag cattctatca gtgagttact tgccctggat
    21541 tggccaggtc tatccattga gccattgatt gctcctgaag atttacgttt attgattggt
    21601 tggacgggta gccctgcctc tacttctgat ttggtcgatc aagttcaccg ttcgagagaa
    21661 gataaaatgg tggcttatca gcttttctta aaaaatagta cagaatgtgt caacgaaatg
    21721 atcaaagggt ttaaagaaaa taatgtaacg ttgatccaac agatgattcg aaaaaaccga
    21781 caattactgc atgatttatc tgcaatcact ggggtcgtca tcgaaacgcc tgctttgaac
    21841 aaattgtgta atttagctga acagtatgaa ggagccgcaa aatcttctgg tgcaggtggg
    21901 ggcgattgcg gaatcgtaat tgttgaccag aaatctggca ttcttccttt aatgagtgca
    21961 tgggaaaaag cagaaatcac tccactgccg ttacatgtct atagcgatca aagaaaggaa
    22021 aaccgatgaa tcgaaaagac gaacatgttt cactagctaa agcctttcat gataaacaaa
    22081 aaaacgaatt tgacttcgta cggatcatcc ataacccttt gccgcaaata gcagtttctg
    22141 atgtcgatct aagtacacag gcagttgggt ttacactaag cagcccattt tatatcaatg
    22201 cgatgactgg cggaagcgaa aaaacaaaaa aaatcaacca agatcttgca atcgtcgcaa
    22261 gagaagcaga tttgatgatt gccactggtt cagtcagcgc tgccttgaaa gtcccttctt
    22321 tagccgatac ttatacgatt atgcgacaag aatatcctca cgggaaaatc attgccaaca
    22381 tcggtgcagg gacttctgtc gaaagagcca aagaagctat ccgattgttt cacgcagatg
    22441 ccttacagat tcatttgaat gcacctcaag agttggttat gcctgaggga gaccgtgatt
    22501 ttaccaattg gaaagtactt attcaagaaa ctcaaacagc cattgacgtc cccctcatcg
    22561 taaaagaagt cggttttggc atgacaagag aaacgctcaa tgatctagct gctttaggag
    22621 ttcatacagt tgatatcagc ggtcgaagcg gaacaagctt cacccagatc gaaaacgccc
    22681 gtcgctccaa gcgggaactg agttatttag ctgactgggg gcaatcgaca gtctcttcat
    22741 tgcttgaagc aaatgaagca gacacttcta tggaaatcct agcctccgga ggtatccgca
    22801 atgcctatga catatttaaa gccctttgtc tcggcgcaaa tgccgtgggt acttcaggaa
    22861 ccgtactgac gcatctgatg aatcacggcg tagaagaaac tattatactg atgaagcaat
    22921 ggcaagaaga gcttcgtctg ctttatacta tggtcggcgc aacgaatacc gcggccctac
    22981 accaacaatc tctgatcttt tccggaccag tgaaggattg gtgcgaagca agagggatcg
    23041 acttagtgaa gtatggaaga cgcacagaaa agagcgtggg acaaaagtaa agaacacttt
    23101 tgtctcacgc tctaaacacg tacaaacggc ggaagtaaaa gcaactcctt cggaaataag
    23161 ctgaaattca caaaaatttg aaaagcaatt ttcgtgaatt ccttcttatt tcttggagtt
    23221 aaatgcttct gtcccagcct cttttttcga ataaatgatg tttaatcagc tgtccttcgg
    23281 tattcgttca atccgctgat ttcacaacct ctccactaaa cagtgtacta aagctctttt
    23341 aaggttgttt tccgatatag gcaagtatgc ctccgtccac atacaagaca tggccgttaa
    23401 cgaaatcaga tgcgctagaa gccaagaaga cagatggtcc agccaaatcg attggctcgc
    23461 cccagcgtgc tgctggcgta cgtccaacga taaattgatc gaatggatga cgttcaccgt
    23521 tttcttgtgt ttcgcgtaat ggagcagttt gcggtgtagc aatataacct ggtccaattc
    23581 cattacattg gatattgtat tgaccgtatt cagaagcgat gtttttcgtc agcattttta
    23641 atccgccttt tgccgcagca taagcactga ccgtttcgcg tcctaattca ctcatcatgg
    23701 agcagatatt gatgatttta ccgccgcctt tttcgatcat atccggaata actgcttttg
    23761 aaacgatgaa tggggcattt aaatccacgt cgattacttg gcggaagtct gccgcatcca
    23821 tttcgatcat cggtgtccgt ttgatgatcc ctgcattgtt gactagaata tcaactgaac
    23881 ctacatcttc tttgatctgt tgaaccatcg cttgaaccgc ttcttcatcg gtcacatcgc
    23941 agacataacc ttttgcatcg atgcctgctt ctttataatg cgctatacct tcatcaactg
    24001 attcttgatt caagttatta aatacgattt ttgctcctgc tgtagctaaa gagcgtgcaa
    24061 tctcaaatcc aataccatag acagcaccgg tgactaatgc tacttttcca tctaaacgaa
    24121 acatatccat actaaattcc atcattttct ctcccttatg cttattttaa ttgatccatt
    24181 tgcaccatat ccatgtctgt ataggtgata ttttctccgc acattgccca gatgaatgag
    24241 taattgcttg tacctacacc tgaatggatc gaccagcttg gcgaaataat ggcttgttca
    24301 ttgtctacaa ctaaatgttt tgtttcatct ggtttgccca tcatgtggaa cacacgtgta
    24361 tcttcattgg cataatcaaa gtagacataa gcttccatac gacgttcatg tgtatgacaa
    24421 ggcatcgtat tccaagcgct tcctggttct aaaatcgtat aacccatttg taattgacag
    24481 ctttcgcata catttggatg gatgtattgg tagattttac gttcattcag tgtcaggcct
    24541 tcaccagtct ccattggttt tacttggtcg atactgattt ttacatttgg atatttatga
    24601 tgagctggaa cagaactaat atagaattta gctgggtttt cagcatcttg agaagaaaat
    24661 acaacatgtc gtgtttcttt tccgatatag tatccgtctt gttttttcat ttcttctttt
    24721 tgcccatcga tctcgatgaa tcctggtcca ccgatattga tcacacctag ttcacgacgc
    24781 tctaagaagt aatcaacacc tagttctttg tctaatgtga tttctaattc tttttcagta
    24841 ggtgtcactc caccaaagat cattcgatcg ttgtgcgtat aagtcaaact gattttccct
    24901 ggttcaaata ccttttctac taaaaattct tttctcaatt gttccgttga ataatgacgg
    24961 atatcttctg gactgtgtgt ataacgtgtt tccatttctt gcattttctt ctactccttc
    25021 tatcgaatga tttttccaga gccgcatgct aaaaattgtg cgacttcttc ttgactaaat
    25081 tggttgcagt ccccatggat cgtatgcttc aaggcagaag ctgccgtagc aaaatcaatg
    25141 atttcttgcg gagacatatt ggataaaatc ccatggagca cgccacctgc aaaagcatct
    25201 ccgccaccta ctcgatccac gattggggtg attctatgga cttgggattc cacatattca
    25261 tggttcatcc acaatgtccc cttcaattca ttttcacttg ctgaatgaac ggtacgcttg
    25321 gtcgaataaa acacctgaat attcgggaac ttttcctgca ttttttgata ataataaatt
    25381 agaggttgtt cttctgcttc tggcgctttc tcgataccta acaaataaag agcatccatt
    25441 tttccagctg agcagatatc tactaaaggt aaaagctgat ggattacttc gctggcttct
    25501 tgctgtgtcc ataattttcc acgatagttg atatcgaagc tgatcaggca gccagctttt
    25561 tttgcagctt ctatcacctg tttcgtcatt ttttgccatt ttttagacaa agctggggtg
    25621 attcctgaaa catgaaatat atctattcct tggaataact cctctagcga ccattccaaa
    25681 tgatccattt cagcaaaact tgatccggca cgatcataga tcactgaggc ggcgcgttcg
    25741 ccactaccag tttccatata ataagtacct agtcttgatc caccagataa tacctgtttc
    25801 gtatctactc cataacattg caagtgcttt ttgactgctt ttcccaatgc gttatcaggt
    25861 acttttgtag caaaaatcgc ttcatgtccg taattagcta aagaaattgc tacgtttgct
    25921 tcccctccgc cataatgggc agcgaatcgt tcacttgaat cgatacgggt tccctgctct
    25981 gtggacaatc gcaacatgat ttctccgagt gtcactactt ttcccataag cagctacctc
    26041 ttgatttctt tcatttttgc tgcatactgt tgcgcgactt ccgttacttt gtcaaaatct
    26101 cctgtagctg cgggtgctaa aaggttccca cctactccga ctgttacgac accagcatcg
    26161 aaccactctt tcatattgtc cagactcaca ccgccagttg gcatgatatt gacttgaggc
    26221 atcggcgcct tgaaggcaga aatgatactt ggtccataag cgcttccagg aaatagtttg
    26281 acgatatcta ccccgctttt cagcgctgtt ttgatctcgg taatagtcat acatcctggt
    26341 aagtaaggaa tttgatacag attgcacatc tctgccgttt cttgatcaaa ggaagggcta
    26401 acaatatact ctgcgccagc taagatagcc aagcgagctg tgacagcgtc taatactgtg
    26461 cctgcgccaa tcactatttc tggttggtct ttgtagaatg acaaaagttc tttgattact
    26521 tgatctgctt gagggactgt aaaagtcaac tcaataccag ttagaccacc tttgatgatt
    26581 gcgtggcaag cattcaaagc ttcttctttg ctttttccgc gtaccaccgc aatcactccg
    26641 gcgtttttca atcgagataa aatatcaatt tttttcatct tctccaccta tttcctattt
    26701 agaaatttta ttttataact tggaaatcta tacccatatt atacggcaga aaagaaaaat
    26761 tgcaaacgct tttttcatta tttttgaatt ctttgctcga tttcgctttt tgtttgcttg
    26821 agggcactga ttatttgttc ttttgtctca tctgttaaac gatatttcgg catacttaca
    26881 ctaaatgcac caatgatttg ttcgtttttc acgattgagg ctcctataca aaaaatatca
    26941 cgttcgattt cttcatcgtc aaatgcgatg tgggtttctt ttaccgtttt gatttccttt
    27001 ttcaaccgta atgcattggt gatcgtatat tctgtgtaag ggatcaacgg atgcttttca
    27061 agataatcag tgtactgctc ttcactgaat tctgctaata cggctttgcc catcgcagaa
    27121 ttgtataatg gtcgggtgat tccgattttt gaagacatgc ggatcgtttg atgttttgga
    27181 tctagtttgt tcacgtacaa gatctcattg ttgtccaata cccctaagtg gatagtctca
    27241 tcgatctttt gctgcagctg ttctaaaaac ggtaaagtca actctaccaa atcgatttgt
    27301 tcaatgtttt tattcgcgta gcgaataagc tttgcaccta aacgatagtt tttctctgaa
    27361 tttttattta cgtagccgat catcaacaaa gtatctagta ttttcaagac tgtagaagag
    27421 gtcattccca caccctttgc gatcgtctgc aaagagacat cttggttttc cgctaaatag
    27481 tctagtattt tagaggcttt gatcaataca gttccataag gctttttatt ttccatataa
    27541 tattctccta tttctttata agaaaaatta tagcggaaat accatcaatt taaaagagat
    27601 atccagatat aaactaccat ttctctttac aggcaattta atgttgaaga tccgcttcgt
    27661 ttttacatca aactttgata taaagcaatg atttcttcta ctgtcgtttc tcttggattt
    27721 cctggtgtgc atacatctcg caaagcatct tctgcaattg ctggaatatc tgcttctttt
    27781 acacctaatt cagaaatggt ttctgggatt ccaaccgctt tgctcaaccg atcgatctca
    27841 ccacaagccg cttctcttac atcttctaaa gacatttcag ctgctccttt aatccctaga
    27901 acaagagcga tctcacggta cttttctcca gtatattctt tgttatattt catgacagtt
    27961 ggcaaaagtg ctgcacacgc tacgccatga ggaatgttgt accatgcact taacgggtga
    28021 gccatcccgt gtaccagtcc taaaccgaca tttgagaagc ccattcccgc aatatactgt
    28081 cctaatgcca ttttttctcg cccattttga tcgccatcta cagaagcttc caaagaatgg
    28141 ccgatgattt cgattgcttt taaatgcagc atatcagtca tttcccaagc gccttttgtg
    28201 ataaaccctt caatagcatg cgtcatagca tccatacctg ttgatgcagc caattttttc
    28261 ggcattccca tcatcatatc gctgtcgatg aacgcgacga ttgggatatc atgaggatca
    28321 acacaaacaa attttcggtg atttacttca tctgtgatga cgtagttgat cgttacttct
    28381 gctgctgttc cagaagtcgt tggtatagct aatataggta aacatggatt tttcgtatca
    28441 gcaacacctt ctaaactaat gacgtctgaa aactctggat tcgtcaaaat aatcccaatg
    28501 gcttttgccg tatcgattgg cgaacctcca ccgattgcga tcaaacagtc tgcctctgcc
    28561 tctttaacaa aagccactcc atcttttaca ttttggatcg ttggattagg tacgatcccg
    28621 tcgtataaag cataagcgat tgctgcttta tctagaagtt ctgttacttt tgttgctata
    28681 ccgtgctcga tcaatccttt gtcagtaatg acagctgctt tgccgaatcc tcgctgctga
    28741 aattcaggga cgatcgactc aatcgctcct tttccaaaat acgatgtttc atttaaaatc
    28801 attcgattac tcattttcta ttcctcctaa tttgtattat ttttcacaat aaaaacgaaa
    28861 gaaccccaag caaccggttg cccagttgct tgttgctaca gttcaaagac tggcatcaat
    28921 tcccaactga ctggagaggc atctggattt gtctccatga tatcttccat gtacttccac
    28981 catttttgat tgatagatgt atttgccatt ttttcccata acttttcatc ttcgatcact
    29041 aagtaaccaa ataacaaact tgtactcgga tcaagataga tcgaatagtc gatgaccccg
    29101 tgttcacgaa gtgctgtacg catttctggc caaagttcat catgtctttt tttgtattct
    29161 tcttgttttt ctgggtaaac tttcatgcaa aaagcttttt tcaccatgtc agacacctgc
    29221 tttcgtatga acttcctcca aataacctgc ttttggagtg acaccaaacg catctgctaa
    29281 atgccacagg ttttcgtccg tgattgtttg caagattggt ccttgcgctt tgacgtacgt
    29341 atagacttct gctgcctttt ctgctgtctc gatcaaacca aagacttcat ccatgtcttt
    29401 tcctgacccg tagattccat gttgtggcca taaaaccaat cgagtttctt tcattttttc
    29461 agctgttgct tccccaatct catttgttcc cggaaccatc cacggaatga tggaaattcc
    29521 ttctggaaaa acaactaagc attccgtaca catttgccat agcgtacgac taaaggaacg
    29581 ttcgtctaat tcatgcgtaa aactcatcgc taatagatga ctagcatggc aatgcataac
    29641 gatccgatta tctgggtcta cttctaaacg ggcgatatga ctcatgaaat gacttggcaa
    29701 ctcgcttgta ggaaggcatt cgttttccag cccccacaaa agttccagtg ttttgccatc
    29761 ttcagcaact cgaacgattc caagattctc ggcaggttcg ctcgctacat ttttaaaata
    29821 tcttcctgaa ccagttacaa taaaatagca tcctgctaaa cggctggcat caaaaatcat
    29881 cgagatgcgg cggatcactt tttcaggatc caagaacggt gtgatctctt cttcttttag
    29941 taaataagaa atattcccgc catttctttc atcccatccc aagcgataaa gattcgacgt
    30001 cgtttcgatc atttctttca cataaggcgc ttgtaaaata tctatttttt tcaatatagc
    30061 cacactccta ttttcttaat tccaacacgt ctttttgata ttcctggact tcttccagcc
    30121 aatccaaacc aactggaact tgatttttgt aacagaaata attccataca tcggcatacg
    30181 ggaaatcttt caattcttcc gtataagcca ttcttgaagt aaagtcaaaa gccagctcca
    30241 tttctttcaa tcgctcaact ggttccagca tagctttcag cagtgctttt tgtgtatttc
    30301 tcgtaccgat gacccaagca gcgatacggt tgatcgttgc atcaaagaaa tccagaccga
    30361 tatttgtttt ttccaacaat tcgttacgga ctaattcttt agcgatttcc tgcaattcat
    30421 catccatcaa aaccacatgg tctgaatccc aacgcactgg acgggaaaca tgcaacatga
    30481 ttcctttgct gaataaagct aatgaagata atttattgga aatcacttct gttggatgaa
    30541 aatgtccagc atctagacaa acaagtttgt cacgagtcaa cccgtaaccc atataaaatt
    30601 catgggaacc gactgtataa gcttcagcgc ctaaaccaaa tagtttgctt tctactgcat
    30661 cttgtacata ccgttcatct aactcttctg taaaaatttc atcgagtgat tccattaaac
    30721 gttcccgtgg ggtaaaacga tcaatcggat tatctttcat cccatcaggt acccaaaagt
    30781 tattgatact tgtttgccca gtttctttcc caaagtactc cgcgattttg cggcttcttt
    30841 tcccgtgttc gatccagtat tcacgaactt ctttatctgg atgagccaat gtaaagccgt
    30901 ctttcatcat tggatgcgaa aagaaagtcg gattgaagtc aagaccgatt tcttcttttt
    30961 ttgcccactc tacccatttt tcaaaatgtt tcggctcaat ttcatttaga tcgacagatt
    31021 cttctgtatc caaatagata gcatgaaggt tcaatttgtg tttgcccggt atcaacgaat
    31081 aagccttctc cagatcttgt ctaagctctt caggtgttcg tgcggctcct ggaaaattcc
    31141 ctgtcgccat gattccaccg gtcaattcgc catccggtgt caagaaacct ttgacatcat
    31201 ctccttgcca gcaatgcata gaaattttga tgtcctgtat cttttctagt acagcatctg
    31261 tatctacacc gatagttgca tatttttctt ttgcttgttc gtaatttctt tcaatcgtgt
    31321 tcatacgatt actcctttga ttttattcac gtaccattcc atcgtgtttt gcttattttc
    31381 atctgggtaa aaagtttcac atggaaacga ctgtttgatt acatttcttg cttgttctat
    31441 attcgaaaac tgacctgtac tgagcatttg catggcaaca ttcccgattg ctgtggcttc
    31501 actaggtcct gcttctacaa tgattccggc ataatctgct gtcaattgat tcagtagacg
    31561 attattcgat ccgcccccta cgatgtgcag cttttccaat ttcgttccag ttaagtgttc
    31621 tactttgatc agctcttgtg cgtaacaaag agcaagatta tcatacacac aacgagcgat
    31681 agctcccgat gagacaggga ctggctgctc tgtttcaaaa cagtattgct gaatctcttc
    31741 tatcatattt gggggattca aaaatcgagg gtcattgatt tccatcactt gctgcagcgg
    31801 tttttctttt gcagcaagct ctgccaattc cgcataactg tactcgtact cttgcattcg
    31861 ggcaacttcc tggatcaacc acatccccat gatgtttttc aaaaaacgca ccgtgttatg
    31921 ggcaccccat tcattcgtat aattttcaga aaaagcattg cgagaaacat tggcgatctt
    31981 tgtttcaatc cctaaaagcg accatgtccc actgctgata taaccccaac gttctccttg
    32041 acctggcgtt cctaatacag ctgacgccgt atcatgactt gctactgtga taaaggtcac
    32101 atctggcaaa tcatattttg aaaattgttc tttttgcaga tctcctagta tcgttcccgg
    32161 ctctgtcaat ggtggaaata attccttcga gacaccgatt ttttcaagaa gttctatttc
    32221 ccattctttt gtccctgcat tcagcagctg catagtagat gcattagttt tctccgtcac
    32281 catgttacca gtaaagacat accctaaata atcaggtatc agtaacaatt tatgtgcttt
    32341 tgccaaattt tctttttctt cagcaaacaa ttggaacaag gtattgaaag gctggatctg
    32401 aatccctgtc tgctcataca acttatctag agaatagatt tcagaaaact gttctactgc
    32461 tccattggta cgcttgtccc gataagcaat cggatcattt atccgatccc cttgttcatc
    32521 aatcagacag taatcgactg cccatgtatc gatccccgca aaaaggcgat caatccctgt
    32581 ctgctttaat ttttcaaatc caatcaaaat ctctttgata agataatcga tcttccagcg
    32641 atcgtgaccg tctttgttcc gaaagccgtt tttgaaccgg tgtacttctt ccaagatcaa
    32701 agaacctttt gcatccctcg tacttttcat tagtcgtccg cttgatgcac cgatatcgat
    32761 ggcaacaaat gttggattca tttctccacc tcttttactt tattgttgat tagcacttta
    32821 ttaataaagc gttatcattt attgacaatg ctatcataat acagaatctt atattttgct
    32881 tcacactatt ttggtatata attgcattat cttgacaagt tgggagtgtt tacatggatt
    32941 actgggaaaa agccaaccac tcatggtcgg aagactcaaa aagagtgatc ttgacgccta
    33001 cacaaagaag caggaattta tttttttata tccaagaaat cggttacttc aaagcaccaa
    33061 tcccttacta tactgaacga gcacatttac cttcttactt gataaagttt actttgtctg
    33121 gcaaaggttc gcttacatat aatgataatc agcaacagct aaaagctgga gacttatttt
    33181 ttattgactg ccaagaatat cagcgttatc aaacgatcag cgaagaacca tgggaaatgg
    33241 attggatcca tttgaatggt cagaacgtcc aatcatttta tcaggagttc acaaaagacg
    33301 gccataacac cttccattca acaggagatc catcagaaaa caagatccac catttgatca
    33361 cagaattgat ccacatgcaa caaggcacga atgcccgtac cgatttccag tcctctgtag
    33421 ctatccacga gttgctgaat gaactgattt tgcaaaagta tcagctagat ttcgaagaag
    33481 aggatattcc cgcctacgtt tacaaactga agcaatactt agacgaacat ttcaaagaaa
    33541 cgattacact tgatcaatta gaacaacaat tcttattgaa taaatatcag ctgagcaaag
    33601 atttttccaa atttatcggt tctccaccaa tcgattattt gatcagcaaa aaactttctt
    33661 acgctaaaga ccttcttcgg tatacagaat ttacggttcg aatgatttcc gaagaaatag
    33721 ggattgagaa ttttgcttac tttagccgcc tgttcaaagc aaaaacagga ctatcgccga
    33781 gagactttcg taaattcagc tgattcatat aatatgtacg tcattttttc aaaatatttt
    33841 gaaatatata gataaaaata aaataaccgc agtttctgct tctatgtaaa caacagcagc
    33901 tactgcggtt attttttagt gatcattctc attgttcatc ttcaaaatgt tcataatgaa
    33961 ccaactgttt ctgtgtatca cgtttcaaaa attctttagg cgttatgccg aactctctct
    34021 tgaatgcttt cagaaaatgc ggataagaag aaaaaccact tttactatat acagcactag
    34081 agctgcggta ttctcgcaac aagtgcttgg catagatcag ctttttcttc attacatact
    34141 gatgaaacgt aaatccagtg tactttttaa attctcgtgt gatatggtat ttgctgacaa
    34201 aaaactgctt ttccatttcc tctaaagaaa gcggatgaga taaatttttt gagatatatt
    34261 gtaacatttg ttccacacgc tcattaggaa ctacagcatt ctgttcttgc gcactctctt
    34321 ctttttgtac aacttgattg agaaaaatca agtattctac cagagcttgt tcatatttta
    34381 tgtcttcacc gtaaattttt ttatcaggag gatggtcgat aaatgataat ttttgcttca
    34441 atacttcctg ttctatttgc aatacacgac tcctcttctc tcccattggt tgaaaacaag
    34501 ccgatagatt agaccatggc gtggaacgac tttctagaaa agaaggggtg acaaagatat
    34561 agacacgctc gaatacatct gaagattggc ggacgatacg atgcaaatca ttggaatgga
    34621 tgagcagcac acttcctgct ttgatcgtaa attgtcgtcc gttcaaataa aaagtcgctt
    34681 ctccctttaa tgtagcatgg atctcataga aatcatgatg atgatagatc gttttatttt
    34741 ggctgacgac atctttgaca tgatagattt catactgatc agaaaccatc gtattgactt
    34801 gttcaccgct gattttcttt tggagcatgt gccgcacctc cttttgttca cttatgcaca
    34861 attttattat accacgtttt ttgtaatcaa aaagagctgc ttttatatac tcttataaaa
    34921 aagaagagac gtggcatctt ctagccccgc cccctccatt ctaaatatta gttaaccaac
    34981 gtcttcttta cattattcaa gaagacttct tccataacga atcccatcat cttttgtttc
    35041 ttcaagaacc acatacaggt acggacgatt cttcatgatg atttctttga tttttgtgta
    35101 ctcgatcaca ccgtctccca aatttaccgg cacgattttt tcctgttcga cacgaaaatc
    35161 tttcaaatga accgcacata tcttttcacc aaaacgttca aaagcttctt ctaccaattg
    35221 gacttgttct tgataatcac tacttgtgat caaattggtc ggatctaaaa tgattcctag
    35281 atagtccgaa tctactgctt ggatcagctg ctctaccctt gctaaagaat acaccggatg
    35341 attcaaacct ggttctatcc ccaccatcat ttggtatttt tctccagctt ttaccaagcg
    35401 acggataacg gacacagctt cagcaaatgc ttcatctgtg aaattttctt ccgtatattg
    35461 gatttccggc aaaacacatc cagtttccga agcaaccatc gacgcaccaa aggaagaagc
    35521 atagcgaaga tactgctcga actttgtcag caactcttgt cgtatcgtta aatctggatg
    35581 aatcatattg atatagcaac ttaaaattcc tacggtcact tcttgctttt ctaaaactcg
    35641 tttgacataa tttcccatcc cagaattgat ctcatttgcg ccagaaggta tttcaggaaa
    35701 cgaaagacct aaggcgagct gcagtgtatg aatcccttgt tcttttgttt tttctgcaag
    35761 atcttctatc gtttggactt cggaaagatc atgtcctcta attgtaatgt tcaacataac
    35821 tattctccta actcagcata ttgggtagcc acatgacgat ttgcgggaag taagtaatca
    35881 aaaacaagac gactaagata accccataaa aaggaagaag cggtttcagc attgcctctg
    35941 cttttacgcc tcctacactt gcaccgacat atagtcctgt acctactggc ggcgtgatgg
    36001 agcctacaca cagattcatg attgaaaaca acccgtaatg tacaggatca acccctacac
    36061 ttgtgacgat tggtaaaaat attggtgtaa aaatcaagat agcaggcgcc acatccataa
    36121 acatacccac cagtaataac acaagattga ctaataacag aataataaat ttattatccg
    36181 ttacgcccat aatcaacgaa ctaattgcct gaggaatacc ggtaaacgcc atggcgaaag
    36241 acatcatgga agaagtagcc aataagaaca tgatcgtccc tgacattgct actgattgaa
    36301 acaacatatt aggtatatct tttagtttta ccgttttata gtaaaacata gcaatcaaca
    36361 gggagtaaat gacagctatc gctgaagctt ctactgctgt aaatatcccc gtcaagatac
    36421 caccgataat gatgacaatc aatagaaggc taggaatcgc ctcttttatt atttttccag
    36481 catgagccat ctgtcctttt tcgacaacag ggtatttatt ctttttagca acgatgaatg
    36541 ctactagcat gattcctagc gcccacaaag cgcctacgag ataacctccc ataaacaaag
    36601 cagagatcga agcaccacca gcaacaagtg aatacatgat aaaagcagta cttggaggaa
    36661 tgaccatacc tgtcggagct gacgcgatat tgaccgctgt cgcaaacttc cggtcatatc
    36721 cttcttccac ttgctgaggg atcaacactc cgccaatcgc ggtagaagcg gcaatggccg
    36781 agctagagat ggaaccaaat aaagcattcc ctaacacatt cgtatgagcc aaagcccccg
    36841 gcacgcgccc gacgaacaac atcgcaaaat ccactagttt cttagctatc cctccattat
    36901 tcatgataat gcccgagaga acgaaaaacg gaatagcgat cagcgaaaaa ctattgatac
    36961 ttcccaccat tttttgagct gaactaaaag cagaaacatc aaacggtata actaacaata
    37021 aagtcatcat cgatgagaat gcaatactta tagcaatcgg catccctaat acaagcaaca
    37081 caacaaatac aaaaaataac actaatccgg cttctattgc cattttattc ctccattttt
    37141 atattttttc ttgaattgct ggcttgttgc ttaatgtatc gattagattg atcaggctat
    37201 agataatgat gagtaccccc gatacaggta ctgacagata aacatatccc ataggaatac
    37261 ttaaagaagg cgagatctgc cccattgtca atgtgacagc attccatccg ccatacacca
    37321 tgatcatcgc agcaaatacc agaaataaac tttgaacaac aacttctaaa attttacttt
    37381 ttttgccagt taatttttca cttaaaaccg ttacggctaa atgtgctttt tgtcccacaa
    37441 catacgcgga agacaacatg gcaaaccaga caagaccgaa tcgaacaagc tcttccgtga
    37501 ccgtatttgg atttcctaaa accgttctgg aaaatacttg ccataaaaca acagcgacca
    37561 tcccaacaag caaaacagct cccaggatct ctaatacctt gtttaaaatc attttcatca
    37621 cagctacgct ccttgctctt ggatcttttg ataaaggtct ttgtaggtag gatcgttttt
    37681 gaatatatca tgaagtgggg caactgcctc tcgaaacggt gtattatcta catcattgaa
    37741 ttggactcca tgttcttctt gtgctaaagc tttttcttct tcaattgctt ttgcaaaagc
    37801 ttcaatttca aaaaccgttg attctttcgc tgcttcttca atggcttttt cctgctcatc
    37861 agttagctgt tctttgatgg catcattcat gatgatgata tctggcacac gtgtatgctc
    37921 atcataggaa tagtatttag ccacaccacc atgtccagct gtatacaaga caaattcatt
    37981 gttttctgat ccgttgatca aattggattg aagagacgtg tacacttcat cacttcccat
    38041 aggtacgggc gaaccgccta aaagctctac cattttgata gcggtttcgc tttgcatcac
    38101 acggatcttc tttcccttca aatcttccgg cgtatggatc ggaccgtcta ccatataaaa
    38161 gctgcgctga ccagaatcat aataggtcaa tccggtaaat cctaaatttt tggtcgattg
    38221 gtagattggc tccattattt cttcattatc catcacctta aagaaatgtt cttcatcatt
    38281 gaatagataa ggaactgaaa aaatcgaata atctttagag aaactttcca acgctgaggc
    38341 acttactttt gtgaaatcta ctgctcctga ctgtgtcaat tcgatcagtt cacgttctcc
    38401 acccagttgt ccatctggga aatactggat ctgtacggaa ccatctgttt tttcttttac
    38461 taaacgccca aattcattca ttgcatcaat tactggctgg ctgttgcttg cataagcaaa
    38521 gcgcaaggta actatctcat ttacatctgc atctgcgaca gttgccacac tagaattatt
    38581 tccgcagccg cttagaccta gcagacctag gctaagcgaa cttgctgcaa acagcagtga
    38641 tgtttttttc atatgggttt cctttctttt aacaataaga gacgagcaat tctgtcaaac
    38701 aaagcaccgt taacgactgt ccataaggca ttgctgtgat tccgatattc cgataaaaat
    38761 ctaaactgtc ccccattcct gtcccgattg aaacgttttg aacttctcct ttttcatcga
    38821 tttgttcaag caatccttgg atagcacgat aagcgacttc tttatattct tgtggtaaat
    38881 aacgtttatg gaccgctttc aaaataccgt aagcaaatcc tgcagttgca gaagattcta
    38941 cgtaagaaga aggatcatcc aacaacgtat gccataaacc gctttcagac tgataagttt
    39001 ttaatgcacg cacttgtgct tcaagtgttt cgatcaataa tttgcgcaat gcatcttctt
    39061 tcgataattc taagatttca atgatctctg gaatagcaat cgtcaaccag cagtttccac
    39121 gagcccaaaa tgcttcggca tagttatgat ttccttcaaa cgtccagccg tgataccaca
    39181 atcctgttcg tttatccatc aagtatttga tatggatcat aaactggtaa cgagcttctt
    39241 ctaaatattc tgggcgattc aacagtttcc cgatttttgc caaaggaagc actgtcatca
    39301 tcaatgtatc gtcccacaac tgattcttat tttccggtcc atatgtggca tgctgtaacc
    39361 cgtcttcatt tgtccgaggc atgtcattca tgacccattc tgcccattgt tctaaataag
    39421 gcaaatagcg tgaatctttc gtgtcttcat acaagtaagc catagtcaat agcggtgcca
    39481 ttgtattgac gtttttgggc ggagcgcctt cttccatacg atcctcaaac cattcattga
    39541 ccacttgata agcgcgagga ttttttgtca tctggtaatt tttataaata ccatatagac
    39601 cgacaccttg tggccaattc cacactgtcc agcttttgtc atctactttc agtccatcaa
    39661 agtccaacaa aaattcacca ttatcgtctt tgatcgttgt caggttttca acgagcaggt
    39721 caattttttc tttcactaat tctttaggta caaaaatctc ttctttaata atcatcttta
    39781 cgccaccttt ttaaacgctt tcattttata tccttaatat agcagtaata ccgtccacta
    39841 taaatatcgc tttttgaaca aaaactgtct aaaattgacc taaatgttaa ataacaaaag
    39901 taactttcag ttacttggaa attaaaaaaa gaacttggag aaacaacgtt ttccaagttc
    39961 tttttcatct attttcttat cttttttcta cgctttcttt tgcttatccg ttgtaagcat
    40021 caatgatgat ctgtttcaat tcagagatca atggttcttt cggattagct gtcgtacatt
    40081 ggtcttcgta agctaattca gccatacggt ctacggttgt atctagtgtt tcttgtgtga
    40141 ctccttgagc ttttagactc atgttgatgc cgacagattt tcctaattca taaacagccg
    40201 ttgctaaagc ttcgactaat tcctctgttg tttcgccttt cagacctaag aattttgcga
    40261 tatctgcgta atctgtatct gcacggaaat agtcatattt agggaacatc gcatgttttt
    40321 gtggatcttt tgcattatag cggataatat gcggtaaaag aatcgcattt gtccgtccat
    40381 gagggatccc gtattccccg ccgattttat gtgcgactga gtgacaaata cctaggaatg
    40441 cgttagcaaa cgccatacca gccattgtag aagcgttgtg cattttttca cgtgattcca
    40501 tatcaggtgt ttttactgat ttttctaaat agtcaaagac taacttgatt gcttgtaggc
    40561 tcaatccacg tgtataatct gaagccatga ctgaaacata ggattcgatt gcatgtgtta
    40621 acacatccat cccggtatct gctgtgacag atgctggaac tgacatgacg aattgaggat
    40681 cgacgattgc gacatctggc gtcaatgcat aatctgctaa cggatatttc acatgtgttt
    40741 cactgtcagt gatgaccgca aatggcgtta cttcagaacc agtccctgat gttgtcggga
    40801 tacatacgaa ttgtgttttt tctggtttgt cgatcttata tgtccgttta cggatatcca
    40861 agaatttttg ttttgcacca aagaaagatg tgtctgggtg ttcatagaac atccacattc
    40921 ccttagctgc gtccattgca gaaccgcctc caagagcgat tactgtatct ggcttgaagt
    40981 ctgccatcag tttcgttcct gcgtatacgg tatttgtcga tgggttcggt tcaacatctg
    41041 agaagacttc gatcttcaca tcatttttac gtcgtgccaa tacttcacga actgtatcgc
    41101 agtaaccgaa ttggaccata ccagggtcac aaacgatcat gacacgttca acgttttcca
    41161 ttttctctaa gtacaataga gagttctttt caaagaagat ttttggcggt aatttgaacc
    41221 attgcatgtt atttctccgt ttcgctacag ttttgacatt gattaagttt acagcagaga
    41281 cgttacgaga aacagagttc ttaccataag aaccgcatcc caaagtcaat gacggaatca
    41341 tttcgttata gatatcaccg atgccgcctt ctgcagatgg tgtattcact aaaatacgac
    41401 aagctttcat tcgaagaccg aatttgacat gcaactcttc gttttctgaa tggataactg
    41461 ctgtatgtcc taatcctcca agattcaaca taccttcaca caaatcaaac gcatggtccg
    41521 tgttgttagc tttgatcatg gcaagaactg gagaaagttt ttctcttgat agcggataat
    41581 ctgggcctac gccttccaat tcagcaatca gcattttcgt tccttttggt acactgattc
    41641 ctgccaattt tgcgatttcc atcgcagaat gtccaacgat tgatggattg accgcatatt
    41701 ttccttcgtt catcactgca tcttctaatt tttgcaattc atctggtttt acgatataaa
    41761 cttggtgtgc ttggaattct gcttttactg ctgcatagat ttctttatcc acgatcacag
    41821 cttgttcaga agcacaaatc attccattat caaatgtttt cgaaacgatc aaatcgttca
    41881 ctgcacgttt aatttttgct gttttttcaa tataagctgg tacgttacct ggacctacac
    41941 ctaaagctgg ttttcctgta gagtaagcag acttgaccat tccagctcca cctgtcgcta
    42001 agacgatcgc gatacctgga tggttcatta gcatagaagt cgcttcgatc gaaggatgtt
    42061 cgatccattg gatacaattt tcaggagctc cggcagcgat agcagcatca cgtaccactc
    42121 gtgctgcttc tgcagaagat ttttgggcac ttggatggaa agcaaaaacg attggatttc
    42181 ttgttttcaa cgcgatcatt gatttgaaaa tcgttgtgga agtcggattc gttgttggag
    42241 ttactccgca aaccacaccg actggttcag caatttcaat caaccctttt tggacatctt
    42301 cattgatcac accaactgtt ttatcatgtt tgatattatg ccaaatatat tcggacgcat
    42361 acatattttt gattgcttta tcttcgtaaa caccgcggcc tgtttcctct acagccatct
    42421 tcgctaatgg catatgttga tccaaagctg ccattgccat ttcatgcacg atatggtcta
    42481 ctttttcttg atcaaagctt tccatttctt tcaatgctgc atttgctttt tctgccaaat
    42541 catcaatcat tgcttgtacg tcaattgtct tcgttttttc tttttctatt actttagcca
    42601 tcattatcct cctaatgggt catggttatt tgttaaataa ttcacatgta tatcatacaa
    42661 tatttatttt tctatgtaaa cacttttttg tgattttttt cacagttatt tttaaatata
    42721 aaattgcttt atttacaggt gtttgtatag aaaaatatat aaagttattt attattattt
    42781 atatgacaca cccgattatt caatattgag caatatatcc catgctctta tctctttctt
    42841 ttttgaataa aaaatgaggt aggacatctt tctgccctac ctcgtcttta aataagcttt
    42901 gatacttatt tttcttcctt ctcttctgtc tcttccagcg tttctgttgt ttcagttttt
    42961 acttcaactg tctcagctgg ttcttctgtt gtttcaacag tagtcgttgc atcatttgtt
    43021 acgacagtcc ctggtttgac cgttttgatt gctgcacgat cgtactcaag gaagatccct
    43081 tcgcagtcta aaacaactgt ccgtttgtca ttgtcgattt ctgaaacaac tccatgtaat
    43141 ccgccgatcg ttacgactga atcacctggt ttcatgctat ctaacaagtt ttgacgttct
    43201 tgctgttgtt tcttttgcga acgtgacata aaaaaccaca ttgctaatag cgcgataaac
    43261 atcacaagca ttggtaatgt tcccattttt ttcacctcac taattctgta tctacaaaaa
    43321 acactttagc agaaaaatgc tgataacact agttttttac ttaattttta aaagattttc
    43381 ctttagaagc tttttgcgtt ttctttattg aacccgtatt cttcaaaaaa ggcttctcta
    43441 aattcaagca aattatcatc catgattgct tgacgtactt gtttcattag attcaacaag
    43501 aagtataaat tgtggtaaga agtcaaacga atcccaaatg tttcatcaca tttgatcaaa
    43561 tggcggatat aggcacgtgt atagttttta catgtataac aatcacattt ctcatcaatt
    43621 ggacggaaat catgggcata tttcgcattt ttcactacta gacggccttg agaagtcata
    43681 catgtgccat ttcgagcgat tcgagtaggc aagacacagt caaacatgtc tactccacgg
    43741 ataacaccat cgatcaatga atcagcagca ccgacaccca tcaagtaacg tggtttgttt
    43801 tcggggatca acggtgtagt gaattctaat acacgattca tttctgattt aggttccccg
    43861 acagataacc cgccgattga gtaaccaggg aaatccatcc cgactagatc tttcgcgctt
    43921 tgacgacgaa gatcttcaaa ccctgctcct tggatgattc caaataatcc ttgacggtct
    43981 ggattagcat gagctttcaa tcctcgttct gcccatcgag acgtacgttc tactgatttt
    44041 ttcacgtagt cgtaactttc atcgaatggt ggacattcgt caaaactcat catgatatca
    44101 gagcccaatt tattttggat gttgattgct ttttctggtg ataaaaacat acgcgaaccg
    44161 ttcaagtgat ttttgaaatg cacgccttct tcttcgatat tccgcatatc acttaatgaa
    44221 aaaacttgaa agccgcctga atctgttaaa atcggttgat cccagttcat aaatttatgc
    44281 agtccgccag cttcttctac taagtcttca ccagggcgta gccataaatg atatgtatta
    44341 cttaaaataa cgccagcacc catttctttc aactcttctg gggacattgt ttttactgta
    44401 gctaatgttc ctactggcat gaacattggt gtggggaaag ttccgtgtgg cgtgatcaat
    44461 tcccctaaac gcgcgccagt atgtttttct ttcttgatta aacgatagcg aatggcaggt
    44521 tgcgtcattc atttccctac tttctatata aaatatcttg acaactccca ccatcataat
    44581 cgtttcaagt gctttttgca agttccctcg gcttccttct actttattta atattctttt
    44641 ttttatcctt tttttatgaa taacaaaaaa aagaaaaaca ttatgacaga aatatttttt
    44701 gatacatggt atattggtgt tattaaagaa aaggagggaa aaatcatgca atcttctgca
    44761 gaattaaaac gccaagcaaa agagagtcta aaaggacgtt ggggacaagc tgttttacta
    44821 aatctaatcc ctacattgat tggtattgct actattcttc ttattatgat tcctattatc
    44881 ttgtttgcca ccaccatata tgatggatca gcgagcgtct cagaagcgac aacttcagga
    44941 ggcggtagtt caggaggcgg tggcggaatt atttccagca tcattagcgc tttgttcatg
    45001 agcggtattt cttggactta tcttgatatt ttacgtggac aaaaagatca gatcgaacca
    45061 ttcaaagatg catttcgtgg attcaaaggt ttgtttatcg gcggagtgat cagcttagct
    45121 gtgctgatct cgatctttac aacattatgg ggtttgctat tagttattcc tggaatcatt
    45181 aaaagctatt cttattctca aagctatttt atttactacg atatcgtcca agaaacagga
    45241 caaacaccta aagtactgga tacgatcact gcaagtcgaa aattgatgga tggttataaa
    45301 ggacaacttt tctggttaga tctttcattt atcggctggc atatcttagc attagcaact
    45361 gctggtattg gttatctatg gctcagccca tacatttctg caacaaaagc agcattttat
    45421 gaacaattgc caaaagatat ttaaaagaga gtgtgacttg tgaccgaggg ggtggcagga
    45481 gaacaccgtt tatcgagagg cagtaaaaga aacattcagc ccattagcaa aaaggaggtg
    45541 tgacaacatt catgtcacac ctcctttttg tatagttcca gtaaaaagaa aatcaactta
    45601 gaaacagtta atcattcctc ttcttctgct tccggtaatc gatggaactt gatcagttca
    45661 ttcaccgcga ttggtacgat agataaaatc cctaccaaga accagtgatt caaactgatg
    45721 tgtactaggc cgaatagttc ttgcgtaaat ggcagcaaag caacagccgt tgtaatagct
    45781 aatgccagta acgccatttc taccaaagct ttattggtcg ctaaattgac tttgaagatc
    45841 gattggctgc tgcgtacatt gaacacatgc agaatcgatg aataagctaa gatcaagaat
    45901 gcaacagttt gaccgacatg ctgacttgcg tctacccatg ggctgatagt atcgacataa
    45961 ctacctaaat aataaccaaa caaggttacg atggtgaaga cccctgcatt tatggcaatt
    46021 ttttgcaata aacctcgagc aaagatccct tcatttttcg gaatgggtgg ctgatccatg
    46081 atgccgtgtt ccgctggctc tcttcctaaa gcaaatcctg gaagtccatc agcaactaca
    46141 ttgacaaaca gtagttgcac agctgtaaat ggtgctcccc atcctaacag catcgcaatc
    46201 aaaacaataa agatctccga aatattacag cttagcaaaa agttgatagc tttacgaata
    46261 ttctgataaa ccgaacgtcc ttgactgaca gcgtctacga tcgtcgcaaa gttgtcatcc
    46321 gtcaaaatca tatctgcggc actttgcgcc acatctgttc ctgtgatccc catggcacac
    46381 ccaacgttac ttgctttcaa cgctggtgca tcattcacac catccccagt catcgctacg
    46441 actgcacccg tacgttgcca agatttgacg atccggatct tgtcttctgg cgttacccga
    46501 gcatagacag agagagcttt gacacgcgca tctaattctt cgtctgataa ttgatgcagt
    46561 tctgatcctg aaagtgcttc tgacggatca ttcaagatac ctaattcttt cgctatggca
    46621 ctggcagtca ccacatgatc tccagtgatc atgactgttt tgatgccagc ctttttcgct
    46681 cgcttgattg ctcctttact ttctggacgt ggtggatcga tcattccgat cagaccaatc
    46741 aaacgtaagt ctttttccag tgcttcagaa gaaatatctt gcggttcttc gtcataagta
    46801 gcataaccaa cagcaatcac tctcagtgcc cgtttaccaa agcgatcatt aacgatcgcc
    46861 gcttgttcta catcgcctga actaaagcgc ggagcaagaa catcgaatgc tcctttggtg
    46921 attgagatgt atttttcgcc catttggtga actgttgtca tcatttttcg ttcagaatca
    46981 aaaggaagct cgtttactcg tggatatttt tcttctaatt ctgcctttgt atgataattc
    47041 tcttcaacag cccttacgat ggctacttct gttggattac ctttgataac aaggtcgtca
    47101 tcttcttgct cgaccgtcac atctgtacat agtgcagcca ttttcaatat ttccatcgcg
    47161 ctgttcgtca ttgaatcttc gatattcgtt acttcatctt ctttgctcca tacccggcgg
    47221 acgcgcattt cattttgtgt cagcgttcct gttttgtctg agcagataac attagcggtt
    47281 cctagcgttt caactgctgg caactgtcgg atgatcgcat gtttcctcgc catcttctgc
    47341 acaccgtatg caagtgtcaa cgtaacaata acagtcaagg tttccggtac agctgcaaca
    47401 gccaaggaaa tcgaagtcat gaacatctca aggagtggtt ctccttgcag ttcaccaatc
    47461 acaaatacta aagccgcagc cgctaaagcg atcatactga ttcgtttgcc tagctgattc
    47521 aatcggacct gtaaaggtgt tttttgcata gtattttcgt tcaaaagatc cgcgatctta
    47581 cccatttctg tagccatgcc aatggcagtg acgatcgctt tcccacgacc tgcagcaacc
    47641 gtacatcctt taaataccat attaagttgg tcacctaatg actcatcttc ttcagcaata
    47701 aatgctgcat ctttttcaac tgcttcactt tctccagtca acgccgattc ttctacacgt
    47761 aattgtgatg cttcgatcaa acgggcatcg gcagaaatag catcaccgga ctctaaaaca
    47821 agaatatcac ccactaccag ttgctctgac tcaacagtgg tcactttgcc gtctcgtatg
    47881 acagttgccg tttgtttatt catatcttgc aaggattcca gtgctttttc tgcgtttcct
    47941 tcttggacaa tcgccaatac cgagttgatc acaacgatag cgatgatcag cagtccttca
    48001 aataaatccc catgttctgt agcaaatgcg gtataaaagg aaatcaccgc tgctacaaga
    48061 aggatgatcg ttgtaaaatc ggacaagctt cgaaagaatt ttttgatcat cgattctttc
    48121 ggcgcctcat cgaatttatt caatccctgc ttttttcgcc gttcatccac ttcttgtgaa
    48181 gacaacccag tatctggatc tgcttctgtt accttcttta cttcttctat tgtctgcttg
    48241 tattcttcca tcctattcct cctttaaaat cttcgtacaa aaaaaggtac ttattttact
    48301 ataacatcct ttttttattt acttcaaata aagggcttat cgattatttg ttaagaaagc
    48361 gtaaaattta aagtgcttat atttgcaaac gctaccaaag gatgctatta tatagatgta
    48421 aggaaggagg ataccatcaa atagacagaa gaatttctcg atagaacagt gtaagcaaac
    48481 aaagttgtta gaagtacagg tttcacccag acatgcgtgt agagtacaaa cctataaata
    48541 gtgaatgcgt atggatgttt ggatcacgaa gtatccgaaa caagaagtaa tgcgatacgt
    48601 aacaattgaa taacgtaaac gtctgaaaat tgaataggaa caagagatgt ttcaaatgtt
    48661 gaagatgtaa cgaaaacaaa atttttgttt aacatgaaga aattcaacac cgtgctttgc
    48721 atgtatgatg gaaagttaca atcatttctg tttatttgaa tccttccgag gtacaagaaa
    48781 gccgagctta tcgagagctc ggctttttct ttgtcataaa atctttgtaa agcttttttg
    48841 ttattcaaaa ctagcggctt ttcataacct tatttcacga acatcgcatc tccaaaacta
    48901 aagaaacgat agcgctcttc aatcgcatgc tggtaagcag ataaagtcaa ttctcttcct
    48961 gcaaaggcac taactaacat gactaaagtc gattttggca aatggaaatt agttgagaaa
    49021 gcttctacca ctttgaattc atagccaggc gtaatgaaaa tatcagtcca tccgctgtct
    49081 gctttgattt ctccattaaa tttcgttcca atcgtttcta atgttcgaat tgaagttgtt
    49141 ccgacagcca cgattttccc gccattttta cgtacttggt tcaatcgctc agccgcttct
    49201 tccgtcaaac ggtagaattc actatgcata tggtgttctt caattttatc tacgctgaca
    49261 ggtcggaatg tacctaaccc tacatgtagt gtcagataga ctaattcaac accctttgct
    49321 tcgatctcat caagtaattc tttcgtaaaa tgcaatccag ctgttggcgc tgcagcagaa
    49381 ccattttctt tcgcatagac agtttgatag cgatctgggt cttccagacg ttctttgata
    49441 tatggaggca atggcatttc tcccaatgat tcaagaatct ctagaaaaat tccttcatac
    49501 tcaaagtcaa tgatccgtcc gccgtgttcc aattcttctt tcactactgc ttgcaatctg
    49561 ccatcaccaa aagagattct tgtcccaact ttggcacgtt tcgcaggttt gatcaatgtc
    49621 tcccacgtat ctccttgtgt attcgttaga agaaggactt ctaaatgcgc accagtatct
    49681 ggtttttctc catataaacg agcaggcaga actcgtgtat tattcataac tagcgcatca
    49741 ccaggctgta actcttcaat gatttcatgg aaatgtttat cttccatctc tccagttttc
    49801 cgatccaaaa caagtaaacg agaatgatcg cggtctttta atggtgtttg tgcgattaat
    49861 tcttccggca agtcaaaatc aaaatcttct gttgttaaca tatcgtcact cactttctat
    49921 catgtgcttc atcattctat catgtttttc gttctgttgg caaaatcagc actttttcac
    49981 ttctattttt tgttttccta aataaaaaaa ctaccctatt tgtatttttt cattgtttta
    50041 ggcatactag taatagatag ttcttcattg aaaatagaaa aagaggtggc accatgattg
    50101 aatttcaaaa tgtgtcaaaa atttataaag gtggaaaagt cgctgtagag gacgtcaatc
    50161 tttcatttga aaagggtgag ttcatttgtt ttatcggtac gagcggaagc ggaaaaacga
    50221 cgacgatgcg gatgatcaac cgaatgactg atccaacaca agggaaaatt ttgattgatg
    50281 gaaaatccat ccaagaaatt gatccagtgg aattgcgacg tcagatcggc tatgttatcc
    50341 aaagtattgg attgatgcct cacatgacga tccgtgaaaa tatcactctt gtccaaaaat
    50401 tgttgaaagt cagtcaagaa gaacggaata aaacagctga aaaaatgatt gatcttgtgg
    50461 aattgccccg tgagatgtta gatcgctacc cacatgaatt atctggtggt cagcagcagc
    50521 gtattggagt cgttcgtgcc cttgcagcga atcaagatat catcttgatg gacgagccat
    50581 tcggtgcgct tgatccaatc actcgtgatt ccttgcaaga ccttgtcaaa gatttacaag
    50641 agcgtttggg aaaaacgatc gtatttgtaa cccatgacat ggatgaagcg attaaactag
    50701 caagccgtat cgccatcatg agtgaaggcc gtgtgatcca atttgataca ccagaaaata
    50761 ttttacgtca tcctgcaaac gatttcgtag aagagttgat tggagaagat cgcctactac
    50821 aagccaagcc aaatgcgaca agagtcggcg aagttatgct aaatacagca atcacgatta
    50881 caccggaaaa atcattacaa gaagcaatca aactgatgag ggaaaaacgt gtagatacgt
    50941 tgttagttac cgataattcg aatgtattaa aaggatttat cgatgtagaa acgttgaata
    51001 aaaaacgagg aaaagtatcc agtgtcggcg acattttgaa taaacatgtt ttttacgtaa
    51061 aagaatccgc ctatcttcgt gataccttgc aacgcatttt aaaacgcggg ctgaaatacg
    51121 tgccggtcgt tgatgaacag aacaaagtcg taggtatcct gactcgtgca tcattagtgg
    51181 atatcgtcta cgatgtgatt tggggagaag atcccgcaac tactgatgtt gcagagtcag
    51241 ctactcctga agaatctaag gaggtctgac ctatgcagca gttttttcat cagcacggtt
    51301 cagaaattct ctcaaaaggt tttgaacatc tttatatttc aggacttgct ttgatcattg
    51361 gtatcatcat tgctgtaccg cttggtgtct tactgacacg ttttccaaaa gctgccacga
    51421 ttgtgattgg cttaaccagt gccttgcaga caatcccttc tcttgcttta ttggcattga
    51481 tgatcccgct atttggcgta ggtaaattac ctgcgattat cgcactcttt atttattcat
    51541 tattgccgat tttacgtaat acgtacattg ggatgaaaaa tgtcgattgg aattatcggg
    51601 atgtggcaaa aggcatgggg atgactgagt tccaatccat tttttccgtt gaattgccca
    51661 ttgctatgcc aacgatcatg gctggtattc gtttagctgc cgtttacgtg attgcatggg
    51721 ctacacttgc ctcttatatc ggcgctggtg gtttaggcga tttgattttt agtggactga
    51781 ataattacca accagatctc attttggcag gtacgatccc tgtaacaatt ttagcactat
    51841 tggctgattg gcttctaggt ctcttggaac accggctgac accgatcgca cttagagagg
    51901 agaatgaaga atgaaacgta tactaaaaat gaccatggtt cttttctctc ttcttatact
    51961 tgcgagttgt tccttccctg gtcttgccag caatacagat gaagacacca tttcgatcac
    52021 aggtggaatc acttcagaag cacagatctt agcaagtcta gttgccggaa tggttgaaca
    52081 ttatacagat aaaaataccg caatcatcaa caatctggct actacaacta tcaaccatca
    52141 agcgatgatg aacggagatg cttcaatttc agcggctcgc tacacaggaa cagatttaac
    52201 gactacattg aaccttcctc ctgaaaaaga tccgaaaaaa gcgtttgcta ccgtgaaaga
    52261 tgaatttgag aaaagatacg gtcaaacgtg gttcccgtcc tatagttttg aaaatacgta
    52321 tgtctttctt gttcgaaaag acaccgcaca aaaataccat ctatctaaag tcagcgatct
    52381 gaaaaatgta gcggatgaat tagtagctgg tgtagatact tcttggatca atcgtaaagg
    52441 agacggttat gacggcttcc aagaaactta tggcttctcc ttcaattcga tattacctat
    52501 gcagattgga cttgtttatg acgcagtcga agcaggaaaa atggatattg ttcttggcta
    52561 ctctacggat ggacggatcg ccagttatga tctagtcatg ttggaagatg atcgtcaatt
    52621 cttcccacca tatgatgctg ctccagttgt agacaacaag atattagctg atacgcctca
    52681 tttaaaagaa gccttaaata agttaggtca taccatcagt acggaaaaaa tgcaggaatt
    52741 gaactacgaa gcagataata atctagtgga accatcagta gtagcacaac gtttcttaca
    52801 agaaaaccac tattttgaag ggaagtgaag caattggata aaatgaatgt ctttcaacaa
    52861 tttttctatt atttccagga aaatggctcc tatatctttg cacaatttat ccgtcacttc
    52921 ctgatttcta tctatggtgt cctctttgca gcagttgtcg ggattccggt tgggatcatg
    52981 atttctagga gacgtaaatt agcaaactgg gtgattcgtt tggcgaatat catccagacg
    53041 atcccttctc tagccatgat ctccatttta atcattggat taggcctggg agtcaatgtc
    53101 gtgattgtta ctgtattttt gtattccctc ttaccgatca tcaagaatac gtataccggg
    53161 atgatccaag tagacaaaaa catccttgat gtcggcaaag gcatggggat gactgctaga
    53221 caacgactat tcatggtaga gttaccactc tctgtttctg ttatcatggc aggcataaga
    53281 aacgcgttag ttgttgccat cgggataacc gcgatcggtg cattcgtcgg tgctggtggt
    53341 cttggagata tcattatccg aggaaccaat gcaacagagg gcacatcgat tattttagca
    53401 ggtgcattgc caactgcatt aatggcgatc atcacggact ggcttttagg tattttggaa
    53461 agacggctag atccagccag ccgtacatca cgctgatgtt attgtaaaaa acaaaagctt
    53521 ctgcgaccat ttgttggtcg caaaggcttt tttagttctg tcatttttaa ggcaataatc
    53581 cactctgatt cttccctcct tatgggcaaa gagttgcttt tcttagctat ttatccattc
    53641 cgtatactcc aacttcatac tgccaacggg ttcatatctg catccaccga ctaagttttt
    53701 ttcttatccg ctaaggcatt ttgccaatcc attttcccat cactcgtcat ggaattcata
    53761 ccacatgatc tctaaatact cgtctgcttg ttctttgaat tgggcatcaa ttcttacatc
    53821 ctttggcgtg gctttcattt aagccttttc catcagtcct cgattgttcg atgccttcag
    53881 ctgcatcatc aaaactggtg actgccgtat cggtaacttc gatcatcttt tatacaatcg
    53941 ggaacttgcc atcctcatcc ttggctaaaa acatatctag ccattccagc ttctcgccat
    54001 gggaggtttg cggttcctaa aaccggtaca cgattcttat ggactgcatc cgtcgcaact
    54061 agacttggcg atacgatgat ccttcaccag aagagcaccc ccgtaaacca attgatcgat
    54121 atattgccaa taagaaaaga cattggcgta aaagatatcg atccctcgtg atgcattgcc
    54181 gttgatactg ctattcatgg ccgaaagcgc cacgacttta acttcttccg tttgagtgac
    54241 attcatgttc ggcaatgtcg ctttggatac ccgctctgct aaaggaacga cactggtatt
    54301 gtatttcgta tctgtatctt ttccaaaaga ccatgccaac gaatcctctg ggaaccaata
    54361 agacgactcc ggttgattgt ccattgcttt tttcaacata cttttggctg tttctcgata
    54421 aacgacattt ccaattgtct tcgcctcact ttggcgataa tcgatggtcg ttgccactgc
    54481 caacactaac aacgatgctc caattttact gattttcctt gtcacacttc ttcctccttg
    54541 ttttacataa acccgtcaac attccattcc taaactcaaa caatgaccct tcaaaggtgg
    54601 agatatagga acaaattaaa gaaatcatgc aataaatcca agaaggttgg ttttagaaca
    54661 aaaaaagagt ctcctttttg gggagactct ttgttaacta tctaaaaagg ttggctcttc
    54721 tcacttcact cataatgtgt ccaggcagaa taaatttcta caactttatt ttcctcatca
    54781 actttgtaaa ccactcgatg ctgtctattc agcctgcgtg agtatctgcc ttcatgtgta
    54841 ggtctcagct tttcaaaaga ctgcgtgggc atatatggat cttccttcaa tgtctgaatc
    54901 acttcttcaa attgtttctt taaattcgtt tgcttgattt ttcttaaatc cactttcgca
    54961 gagtttttga ttgcgactgt atagttactc atcaaagagc ttcccaatca atatcatcga
    55021 tattcgtaaa gctactgtcg tctttttcgc ggtctcgtac tttatctaaa gtacctgttt
    55081 gctcaagaaa aagggtttct tgaatcgctc tccagtcttc cagcccaatc aatacagcat
    55141 tgtttccact acgatcacta ataatctcga tttcagtatg attttcattt acctctttca
    55201 ataactgata aaaattttta cgagcagtgc taggattcat cgttgtatta gccattgtct
    55261 ctcctccttt ttctgatata attatagcgt acttgattaa gtacgtcaaa aaaatacaat
    55321 aaagcactta aattcagcaa acttttcctc ttttttacat agagaaaaag accccttttt
    55381 agggaggatc tttgtccacg ctcacttctt actcctttac gactagcggt agccaaacgt
    55441 aaagtaattg gtaacaacgt atcatcttta gcaaaggtcg aatccaaagc ctgtgagtct
    55501 atgcaacaac accaatacac gaaggttcct cgttgcagtg tgacttgatt gatatcttga
    55561 ataacctgag gatacgcacg aaagatttgt aacggttatt caaaactgat tttggccgtc
    55621 aagtcttcta ctgcctcaaa aatgattgcc tctttcgtct cctgtcattc atgttcacta
    55681 tcgatttgat acttgcttag accgttgggt ttgtgaatca ccaattaaaa atggcactga
    55741 gttatggtaa caaaaaatgg tcacttgacg aggctgagat tcctacttat gaagacacat
    55801 ggcacttaag agtcgaggta aaggaagaag ctgaaaatat aggaggctgt acattatttg
    55861 gcaaaggatt cggaaattat gatcaagaca tcaaaatcaa aatgttttac agctaaatag
    55921 gaagaataat aacatttcat acgctgttcc catctttaat ggcttataga gggcaccttt
    55981 agctgagaat aagacaaaac agaccctcta gcttcaatta agagctagag ggtctgtttt
    56041 tgattttaaa acttggttta tttcttcaaa tggtagctat aggtgctgac aacgatattt
    56101 tcacgccaag acaatcgcaa tcttaaccgc aaacttgttt ggttatggag aatattctgc
    56161 cagcgtctgt ttggtacaaa ttgaggaatc aacacagtag ttgtatagtt ttgtttcgct
    56221 gcatttttac ttacgatatc tacataacgg ataattggat tttcaatcga gcgataagaa
    56281 gagtggacga ttgaaaaacg gacatctggg aaatgctttt taaattctgc ttcgatttct
    56341 ttttcttttt cgacatcttc gtctagcgaa acatgcatcg caacgacata atcaccgatc
    56401 gaacgtgcgt aattcaacgc accgatattt acccgtgtca cattcccgac taatacaaga
    56461 acggtattcc cgtcataatc atgtagtttt gcatcatttt ctaaacgaag ctgttctgct
    56521 acttttttat aatgatcatg gatcttataa aaaatgaaca taacgattgg cataatgatg
    56581 aagaaaggcc aaatgtcccc taaccgatag acaaacaaaa tcgcaatgat tgcatatgag
    56641 atgaacgcac ccgtaatatt ggcaatcgat ttgcttaacc atctttttcc ttcttttttc
    56701 catttgacga ccatccctgt ttgagataaa gcaaatggaa tgaacacgcc gatagagtat
    56761 aacggaatca agcgttctgt tgagccatgg aaaataaaca gcaatacgat cgaacctagc
    56821 gctagtgtga taatcccatt ggaatagcct agtcgatccc cacgatcttg atacatgtga
    56881 ggcatgaatt tatcttttgc cagattgtat gcaagaactg gaaaagcaga aaaaccggtg
    56941 ttagcagcaa ctgctaaaat cagcgctgta gcaaattgaa ggacgtaata caaaatacca
    57001 tgtccaaaga cagctttacc aatttgagac aaaacagtga cttctttttc aggaacgatc
    57061 ccataccaat agttgataaa tgtgatcccc acgaagaaga aacctaagat caatgccatc
    57121 atcgtcaatg tagcagcggc attttttgct cttggcttct taaagaatgg tacagcatta
    57181 ctaatcgcct ccaccccagt taaagaggaa gaaccagaag aaaatgctcg taaaatcaaa
    57241 gcgatagaaa cacccggcac agccgctcct ggaagtgcag tagcttgcaa tggcacagca
    57301 cctgtcacga tattatataa accaacaacg atcagtaacg agatcacaag gataaatgtg
    57361 tacaccggaa ataataggaa cgatgcagat tccctcagcc cacgaagatt cagcatcatc
    57421 agtagcaaaa cgattgtcac agaaatagcg acttgatgac cgtagagtgc tggcaccgct
    57481 gatgtgatcg cttcagcacc tgctgagaca gatactgcta ctgtcaacat gtagtcgatc
    57541 aataaagagc caccggaaat caagccagca ttctttccaa gattctcact gctgacgaca
    57601 tatgctcccc cgccatgagg ataagcatgg atgatttgac gatatgatag cgttaatgaa
    57661 atcaataaaa taataacaaa agcggcaatg ggtagcgaat accagatggc tgcagcagac
    57721 aaagcgacta aaacgacaac gatttgttcc gtaccatatg caatagatga taacgcatca
    57781 gacgacaata atgccaaagc agcaaatcgg gttaatttgt gctcatcatt ttcagcagat
    57841 tttaaaggct tacctactaa caaccttttt aagtaatcca atttctaaac tcctttacac
    57901 acaattttac ttcaagtttt atagtcgaaa atttatagta aacaagatgt tatagtacgc
    57961 tcactgacct taaaagtcaa taaactttaa gaaaataatt ggaaaaaaac agaaaaatat
    58021 caagcgcttt ttcaaacgct ttaatacagc aaaaatctaa cattttttta tgggcttttt
    58081 ctttgaattc tataataaaa ggatagaact tgacttagaa aatcaagttc tatccttttt
    58141 gatatcttaa cttttggaag agagatgtaa ggctgctttc tacccatcat tcttactact
    58201 cgaagcatag cgcagttgtc acaacctctt gataaacggt gtttaaaagc ttgcatctgc
    58261 ggtaataagc ccaaccaaat taaaaacata agaagctgtt tttatttggt cgttcttatt
    58321 acttgatgca gagcagcttt tttacaacct cttggtacac ggtgttcaat cagctgaccc
    58381 tcggtattag ctcaaaaaaa cggcaaaata tgaggaacta tttttccatt tttttctcta
    58441 atactcaggt caaaacactg atttcacaac ctcttttttt aatgaaaaat atggctctca
    58501 tgcgccgctt ctttgaattt cgtcattttt tcttcgatga acggatagat tgccgttcct
    58561 acgattccaa agacaacaaa caaaccgatc atgatccaga ttgctttcgt agcatttggc
    58621 caataaattc ctcctgcaga ttcccgtaac aagtttaccg catgagtaaa tggcaagaac
    58681 ggattgatca tttggaagaa tttccctgag acttggatcg gatagttccc gcctccgcca
    58741 gagatcgaca gtactaagat gataatagcg atccctttac cgacattatc gaataatgca
    58801 gccaagacat agacgatcat catgaacgct aaggcgatca agacagcaaa tgacacgctg
    58861 tagaccggat tttggacatc tactcctagt aagaaaatat tccctaatgt aacgatcaag
    58921 ctttgcgcaa tcgccattgt caaaaatgtc agcatacgag caacgaattg ttctcgtttc
    58981 gtaaaattgt ttcggtcttt ctttcctaaa tagtattctg tcgtagcaac acttgaaaga
    59041 agaacagcgc cgacccacag acatagtgca gtataaaacg gggtgctagc tgaaccgtta
    59101 tttgcgattg gataaagcgc attggtttgt agctcaacag gttgtgtaaa gaagtcactt
    59161 tcttttgttg cgtctagttt caataatttg atgacttcgc ctagatcgac ttcttgttcg
    59221 ccttttcgga tcgcgtctgc tgctttttgg ataccagctt tgatatttgg ccagtcattg
    59281 ttgatcaagt ccgcagcagc gttcacagct gtttcgactt ctggcatttt ttcatttact
    59341 gttgtcaatg taccggtgat ttcatttttt agtgctggcc aatcattttg gataaaatca
    59401 gcggccaatc ctaatttttg ctgaatgatc ggtaattcat tgttgtatag gtctgcccct
    59461 tcattgatgc cattgacgat ggtatccata tttccattca gcattgtatt ggcatcatgg
    59521 atttcttgtc caattgcagg catttctttt tgatattttt ctaaaattgc cactgcattc
    59581 gttacagttg ctttcgttga gctgagaagc gaatcaaagt cgatctggtc cgctttgctc
    59641 agtgcgcctt gtgcagtccc aatcgtatcg atgacttggt ctagtagaga agtgacagtg
    59701 gaactgattt gacctgtatt gataccagca atgatgccat tgatattatt aacaacggca
    59761 tcaatttgat ctagcaattc atcaatagcc ttcgtgtctt cattttgaac agctgtgttc
    59821 aaagcagata ggcggttttg cagattgctt agcaagggtt ttaattgatt tagctgatca
    59881 ataattggct gtaatttcgt atttccagca cttgtttgca atttagtcag gaactcgacc
    59941 agtttatcga ttgcattttg ttgttgagat attccgtcaa taaagccgtt caatacattg
    60001 ctgatgtttt tcttttcatc atcagtcagc ttaccatctt caatagctgt tcttacagta
    60061 tctattaacg aacctgtcgt tgttgccact tgtccaattg acttcaatgt aacatctaca
    60121 ctgtttgtga tgcttggtat cgcatctttc agctgagagg cacctgattt tgtttggttt
    60181 gctagatcac ttgcctgatt ttctaaattt ttcacatctg gcaagatatc ttgtacttgt
    60241 tggatgatcg tcaagccttg ttttgcttca tcgattcctt ggttcattgt ctcttggatc
    60301 gaagcaaaat cgccgtcgat ctctgctagt tgcttaccgg cattttggat ctctgggatt
    60361 ttttcttgca aatcaaggat gatttttgct tgatctttca actcaggcat tttgtcattc
    60421 aatgcaacga ctttttcacc catctcatct actttaggga tgtaatccac gaattcattt
    60481 gctttattca gtttttcctt gatttcaggt agttgacttt gtaattcgag gacttgtttt
    60541 gtgtagccgt cgatcgtgtc caaattttca tctgttgata aaatcatatc tttaacttta
    60601 ttgatactta ctaggttcgt atcgatgtcg taacctatct cattgaatac tttcaataaa
    60661 gtcgaactgg ctgttttgat aaattcattt gtgatttgcg attggatcga acttgctcct
    60721 ttatcggtga ttttcggtgc aatcgcattg attttttcat ttaccgtata ttcgattttt
    60781 ggtttcttga tatcaccact agtaaaactc agcagatctt ctgagaaatc ctttggcaag
    60841 taaatacctg cgtaatactt tccggaacgg actccgtctt ctaattgttc ttttgaatca
    60901 acgaattgcc atcctaattg tttattttta tgaagcgatt cgatgacttg ctccccaatg
    60961 gccacttctt taccttgaaa ctctgctggc ttgtctgcac tgtaaacagc aattggcagc
    61021 tctccagtat ttccatatgg atcccataat gctttgatgt taaaccacgc atatagcgaa
    61081 gggatgatca tgatcgcgat gatcagaagt gttgcaatcg ggtttttgaa aatccgtttc
    61141 cagtcaagca aatataatcg aagcgtattt ttgattgact gcatagttct cccccttctt
    61201 cttttttcag ctgaattact ttaccatgta tttcatactt ttttcaatcg gcttttttca
    61261 aaaaagtcga atttttggac atatcacggc aattgtctca ttgtaatctc ttacatattg
    61321 agaaataatt aaggattaca gcctttactt tatcatgaaa taaaagggaa agataatttt
    61381 atgccaaagt ttttatctat tatacgtata cgcaaaaaag ctaaaacatt ttttgtcttt
    61441 gcccctacaa aaatgtttca gcttttttcg ttttttattc ctgtatttct tatttttcga
    61501 atgacaggat gattttccca gcaggaggat gtccttcaac tagctgatgt gcttggatga
    61561 catgcttaag tgaagcagac agattcattg aaataaaaac atgaaaggct cctttttggt
    61621 aggctttgat tatgccagca aaggcttctg catctagata ttcctttctt ggaacaaacg
    61681 attcataaaa gccttccttt ttttgccgca aatctagatc tgggaggtca tttaatgcaa
    61741 catacctgcc gcctggtttc atgatctgga tgccggtttc tcctttgata cttcctttcg
    61801 tcgcatcgat tacaaggtct gcctgatcag caaattgcag acctggattt gttttatcat
    61861 aagcagcaaa tgctgaagct tctaattttt ttaccttttc ctcattttta ctggatgcac
    61921 tggttaagat tcggattccc ttttcatgga gaagctggat cagacttgac ccgaccgcac
    61981 ctgatgctcc tagtatcatt acagtatctg tcggctggat ttccgtcaaa tggttgatta
    62041 gattataagc cgtaattcct ggcgtgacca ttccggctgc ttcttcccaa ctcattttat
    62101 ctggaatttt tgctactttt gctgatggca acacgacttc ttctccgtaa gtcccgccga
    62161 ctgcatggac agctactcga tcacctacat gaacagttgt gacatctgaa ccaatctctg
    62221 tcacaatacc agcgccatca ttacctggta cataagggaa tttcaatgtt cgtatctctt
    62281 tcatttctcc taaacgcaag gcaacatcat aaggattcac actgaatgct tttatttcta
    62341 cacgaacatg tccagaaccg acttctctgg gatgagcatc gatttcttca aaaacatctt
    62401 gtgcttctcc gtaaccgtta attgcaaatc ttcgcatggt caccatctcc ctttttcttt
    62461 aagtttacgt ttatgccttt attttgacaa aaaaaatgtc ttttcgtttt ggcgcatgat
    62521 tcaaaacaaa atagttgtct tttttcgcgt aacgtgttaa attatttatc taaaagcgta
    62581 aagattacga tttacgctaa taaaaaataa ttggaggaat ttgttgatgg ctaaaaaatc
    62641 aaagattgcc aaagcaaaaa aacagcaaga aacgattgaa cgctatgctg ctttacgcca
    62701 ggaattaaaa gcgcaaaaag attacgaagg cttgcgtaaa cttcctttag atgctcgtcc
    62761 agaacgattg aaaaatcgtg atttaatcga tggacgccct cgcggataca tgcgtaaatt
    62821 cgggatgtcc cgtataaaat tccgtgaatt agcacataaa ggtttgattc caggagtaaa
    62881 aaaagccagc tggtaatcca agtctggctg taatgtaaag gaggagggcg catggaccac
    62941 tccttggatc atcaaaaaag atacgttcgc ttcttctagc agaaatgctg gtactagaag
    63001 cagacgtatc tttttttacg cacagctggt ccttttttat atgtaaaggc tgcatggtta
    63061 cttcgctata tttatttagt aacaggagtc agtgcttcga tacccacact aattttcaat
    63121 gctcggtcaa cttctgccat aatgggtcga tccaaatggc agaccttttc tttcaatctt
    63181 gatttatcga tcgttctgat ctgctccaac aggataacgg agtctctttc tattcctgtt
    63241 ccagtagctt tgatcccaat gtgagtcgga agcttaggtt tagccatttt tgccgttatg
    63301 gccgcaacaa tgatcgtcgg gctaaaatgg ttacctagat tattttgaat aaccaaaact
    63361 gggcgtgttc cgccttgctc cgatcccacg actggagaaa gatcagcaaa ataaatatct
    63421 cctcttttta ccataggatc ctccttattc ttgatattct cttggtatcc gctgtcctaa
    63481 tccgcaagcc acttcatagt ggatcgtacc taattgatct gctatatcct gcatagtgat
    63541 ttctttgtct gcatttttgc cgataagcgt cacttttgtt ccgacaggaa attcttgagg
    63601 caatcggatc atcagttgat ccatacatac acgcccaatc gtttcacagt agttcccttc
    63661 taccaataga gaaaaaccct gcatctttcg tggccagcca tctgcataac cgatcggtat
    63721 cgttccgatc cactctgctt caggagtgat atatgtttct ccatagccga ttccttcacc
    63781 tgctgggagt tttttgactt gaatcaattc agaaaccagc tcaagtgctg gctgcaatgg
    63841 ataaacttcc ggtagtgcgt gcccagaggg attaagtccg tacattgcta ctccataacg
    63901 aatcatattg ccgattgttt catcatgcca taaagccgtt gcactattgc tgacatgtac
    63961 atatcgagga agttcgggaa gttttttcag cacttctata aagcgttctt tttgcagatt
    64021 ccaatatgta tcatctgcct gatctgctgt agaaaaatgg gtaaataaac cttcccattc
    64081 taatacacga ctttctttga tgaatgcagc tgcctctttt acttcttcag gtgtacaaaa
    64141 tccgatacga cccatcccag tgtctgcttt tagatgaatc gaaagaggcg tctctagctt
    64201 acttttctct aaaacagcag ctgctgcttg caaccattcc cgtgtacctg cagttactga
    64261 taaatcatga gcaacagcta cagtcagtgc gtcgtaagga accatattca agataaggat
    64321 gggctgagta aatccagctt ctctcaactc gactccttca tctaaatttg aaacacaaaa
    64381 gcccgaagct ccacctgcaa ctgctgcctc agctgtttca atagctccat gaccgtatcc
    64441 gttcgctttt actactgcaa acaattcttt tccttgcgga agtcttgcaa cttcatttgc
    64501 tacattttca gttatagctt ttttatgtat cactgctttc gttgggcggt gccaagcgac
    64561 aaccatcgtt tttccttctt tctagtcttc caaaataatt tgtgcaaaag ctgtttcatt
    64621 tgtatgagta atactgaccc atacattgcc ttcatgaggt gaacgcgtca caacaggctg
    64681 accattttca ttttttaaaa tctcgacatc ttgaaaggta acttttccga tcccagttcc
    64741 ccaagcttta gagaaagcct ctttgcaagc gtaacgcccg cctaaaaatt ctacttggcg
    64801 atgaaaaggc aaggactgga atagtttcat ttcatcagag gtcaagattc gtgcaatgaa
    64861 ttttggtttt tcttcgatca gccgagtgat cctaggaagt tcgactgcat cgatcccaat
    64921 tccttttatc atatgctttt ctcttttcaa taaatttaga acactctcat tctaccaaat
    64981 tataaccatt ttgccacaaa taagtccaca aataacaaaa atgaaagttt ctcttaaaaa
    65041 agagaaactt tcattttatg tttaatcgtt atttgaacgg attacaaatc cgcgtttttt
    65101 atcattgtga cgtttaccgc catctttttg gctgttatta tttttgctat aaccgccttt
    65161 tgatttgttt ccacgataat taccgccgtc acgacgatta cggttgtttc cgccaccacg
    65221 tttgccattt ttattgtatc cttttttctg cattggcaat gggcgttctg gtgtgatctt
    65281 cactggcact gcatcagctg gatcttttgc tgtcgttttt agcaaaagtg caactaaatc
    65341 ttgtgcagaa tattcttcta ataatttatc tgctgtttgc aggtatttat ctagaccatt
    65401 ttctgcaagt tttgtttcga tttgttcaac tgcagcacct aattgacctt tgaatgcttc
    65461 tttttcggtt ggtggacgta aagtcgtcat ccgttttttc gtcaagtttt caatcacatg
    65521 caagtagtcc atttcatttg gtgtaacgaa agtcacagac ataccgcctt ttccggcacg
    65581 gcctgtacgt ccgatacggt gaacgtagct ttctggatct tgagggatat catagttgta
    65641 cacatgagtg acacctgaaa tatctaatcc acgtgcagca acgtctgtcg ctacaaggat
    65701 atctaaatga ccgctcttaa atgaacgcaa tacgctcatt cgtttttgtt gagacaaatc
    65761 gccatggatt ccttctgcac ggtaaccgcg tgcttctaat ccgcgtgcca attcgtctac
    65821 acggcgtttt gtacgtccga agacgattgt taattcaggt gtttgaacat caaacaagcg
    65881 agtcatgata tcgaattttt cgtattcttt tgcgcggaca tagtattgat cgatcaagtc
    65941 agctgtcatt tctttcgctt tgatcttcac atgatgtgga tttttcatga atttaacgcc
    66001 aatattcttg atagctggcg gcatcgttgc tgagaaaagt aatgtttgtc tttgatcagg
    66061 aacttgtgaa atgatttttt cgatatcttc caagaagccc atattcaaca tttcatctgc
    66121 ttcatccaaa actaatgttt gaacggttcc aagtttcaat gtgtggcggt taatgtggtc
    66181 aagcatacgt cctggtgtac ccacaacgat atgtggacga tctttcaagc cacggatctg
    66241 acggccaata tctgctccgc cgtaaacggc ttgtacacgt acttttttgt cgcgccctaa
    66301 acgatataat tcttcttgtg tttggatagc taattcacgt gttggtgcga taactaaacc
    66361 ttgtaattcg tgacgatctg gatcgatttt ttctaacatt ggtaatccaa atgcagctgt
    66421 tttccctgta cctgtctgtg cttgtccaat aacgtctttt ccagctaatg ctagtggaat
    66481 cgtttctgct tggataggcg ttgcttcttc aaaaccagcg cgttcgactg atttcaacaa
    66541 ttctggtgat aattctagtt ctttaaattt caaatgaatc ctcctatatg gttgttaact
    66601 gaggatgtag cttttttgct accccgtcga tttgttcggt caaagtgtat tgtttctttt
    66661 tgacagtggc cacgcacgtt tttatccatt cttagcccct gtggcaccac cgaaaaatca
    66721 ctttgcgaag tttaggcatt agaaaagcca aacgggtcgc gttcagcttt atcattatta
    66781 tgacatctgg cttcaacaag ttgtttctat ggcaaaacca gtccaatttc tcttcatctt
    66841 tcggcaatag gttattctaa catagttttt cgttttctgc aagtttacag atttttattt
    66901 catttgctga agttgttcta ccacttcaaa taagcccatg ctgttgctgc cttttagcat
    66961 aatactatct gttgcttcaa gattttgttt gatagcatca atcaatttat tcttttcttt
    67021 ttcaaaatag aacacaggta gttgggggta tttttggttc aattcttctt ttagtgcatg
    67081 catttgttcc ccataaagga aaatcatggc gaatttctca ggatcgatat gttcactcat
    67141 ttgcgcatgc atatttaaag actctttccc taattccagc atatctgcta acacagcgat
    67201 tctgcgtcct tttgttggca gatttttaaa agtatctaat accagtccca tggctgttgg
    67261 atttgcattg tagacatcgc tcaaaatagc tgcaccattt cctgccgtta gccattcggt
    67321 tctgttttga gtgatagaaa catgggcgag cccttggcgt atctcaggta tggacagtcc
    67381 aaagaagcgc ccgatcgtat acgcaatcaa agcattcgtt acattataac ttccaggcag
    67441 cgggatcgta aatgtttctc cttctactac aaaatcggtt tgtgcttttc cttttgctgt
    67501 cacttccgca tgaacatcgc catttccaat accgaatgtt gtgactgttt gtactaatgt
    67561 tgccgttaaa ggtgtcagca acggttcatc tgcgggcaca acgagtaatc cgtctgatac
    67621 aagtccgtca gtgatttcca ttttggcttt agcgattcct tctcgcgacc caagattttc
    67681 tatgtgtgct tcaccgatca ttgtgattgc tgcagctttt ggttgagcca taagtgaaag
    67741 ttccgtgatt tcattggcgt gatccatgcc catttccaaa acgatctttt ctgtatcttg
    67801 gggcatgtgc aaaatcgtgt aaggaagacc aatattattg ttgtaatttc cttgcgtctt
    67861 atatgttttg tatttttcag acaagacaga cgcggttaga tctttcgtgg tggttttacc
    67921 attgcttcct gtaattgcaa cgacttctgc gcctactttt tccaagtaat aggttgccaa
    67981 ttgctgcatg gctttcaaag gatctgctac aggaataact ggaaaatttt ttggagcttg
    68041 ttcaacaggc aagctccaaa atgaagcaga tgctccgttt gtgatcgctt gctggataaa
    68101 ttcatgtcca tctcgcgtac cagcaagagg aacgaataaa cttccttcgg ttatcttccg
    68161 gctgtcaaat tcgacattgg taatctttgt cagtgcagag cattcgctgc ccacggcttg
    68221 tgcaatttct tgtattgtta gattcattga gatctctcct aaaaaaggtt gtgaaaaaag
    68281 cgtttggcct aaacggcggg ttcgcatgac tctcacttcc aagtaaattt ctctattcgt
    68341 atttagaagc tagaatgaac gcggttacgc aatcactcca gcctcttctt tatttctctt
    68401 tattcatttt tttcaaaaaa agattgacgc tgatggtatc gattcattcc taactggatc
    68461 agttcttcaa tcaaatcacc gtatttcaag cccatatttt cccataagag tgggtacata
    68521 ctgaactccg taaatcctgg catagagttt aattcattca ggaataattc atttttattt
    68581 gtcaaaaaga aatcgcaccg gctcaatccg cttccaccta acatcgtgta agctaacttc
    68641 gcgtactctt gcgctttttg ataaacttct tctggcactt cggctggaat ctgcatttcg
    68701 attttattat tgatatattt tgcttcataa tcatagaatg ctacgtcttt tacgacttca
    68761 ccaggcaaag tcgtccgaac gtcttcattt cctaatacag caacttcgat ttcgcgcgct
    68821 tcaattcctt gttcaacgat tgctcgagaa tcatactgat aggctgttgc taaagcattt
    68881 tgcagctctt ctcggttttc tgcttttgta atgccgacac tagaacccat attcgccggt
    68941 ttgacaaaca tcggataaag caaagaacct tcacattgat caaatacttt tttaggattt
    69001 tctttccatt gattcttaag tactggtaca taaggaactt gcggcacacc agcagcttgt
    69061 aaaatatact tggtcatgat tttatccatg gcacatgcac tggtcaatac gcctgcgccg
    69121 acataaggca tattcaatgt ctctaagaag ccttggatcg ttccatcttc cccgtttggc
    69181 ccatgtaaaa ctggaaaaac gatggctcct tcttctttga tttcgcccgg attgatcact
    69241 tttcctgtaa agccttcctc tgtctgtcca cttgggtccc atgaaagatg caagacatct
    69301 ttgctggcag gtttttctgt tagtaatgga cctttgaccc attgtccttc ttttgtaata
    69361 aatacgagtt gaacttggta ataattataa taaatggcat ttaaaactga aaatgcggaa
    69421 agaatggaca cttcatgctc tgcgctgcgt ccgccatata gtaaagtaat cttcaaaaga
    69481 ttgtcctcct gtattctaaa tcgttcagtg atttactctc atattttagc acaaaacaaa
    69541 aataaacgaa taacttattt ataaaaaaac gagccagctt ctaaaagaac gagcgtcatt
    69601 tattttttgt cagctagatt atttgtcccg ttttttataa aaagttatga taaagaaaag
    69661 gagggatgat gatggcgcaa acaaatccac gcagccgctt ggcagaaatc attcgagtgt
    69721 tcatcaatta caatgtagtg ccaaactttg tacagcaaaa aaatcccgaa caggtaaaaa
    69781 aagcctttga agaactaggc cccaccttca tcaagatagg acagatgctt tctgttcgag
    69841 aagatttgct ttcttctgcc ttcactcaaa cattcaaaac attacaagat agtgtaccta
    69901 gcgacacatt ttcaacggtc aaaaagacga tcgaaacaga actttctctt tcattatcag
    69961 atatattcga tgatttttca aaatcgcctt ttgcctctgc ttctatgggg caggctcatc
    70021 gtgccaagct gaagaacggt gacgcggtag tcgttaagat ccaacaccct aacatcgccg
    70081 aagagatccg acttgatctt caactatttg aacgagcgat ccctttgatc aaatacattc
    70141 ctgaaactag cgtagttgat ctaaaagggg tactccaaga agtaaaacgt tccttgatca
    70201 atgaaatgga cttcttaaaa gaaagtcaaa atggcgaaca attctatcaa aagaacaatg
    70261 gctggaaaga gatccgcagc cccaagattt atgacgcttt ttgttcgaaa aaagtgatcg
    70321 tgatggaaga aatgtctggt aaaaacttga atcatttaat gaacgccgaa aacaaaactg
    70381 aaacttttat cactggaatt caaaacaagc agctgaaaca agaagttgca aagcttttag
    70441 ttgagaactt tatgaaacag gtgtttgatg atggtttttt ccatgctgat cctcatccag
    70501 ggaatctgct attccatgta ctaacaaaag aagaacagac acaagctagc cgaaaaactg
    70561 aaacagtcca tgaaaaagag ttcggttcct ttgcttttcg agcctccact tctgcagaag
    70621 accctgttgc gccatatacc atcaactaca tcgattttgg tatgatgggg catctttctg
    70681 caggacttag gcaaaaactc acacaagccg ttttagcgct gtatacgaaa gatgcttatc
    70741 gaatcgaaaa agcggtcctt cgactttgtc agcaagaagg ctcgtttgat gaaagtcggt
    70801 tccaccaaga actgacaagt tttcttgaac agtattatga ttctcctatt gatgaaatca
    70861 acttgcaaga agtattcaca catgtcgtga cgatttgtca tcaaaataat ctgcagttcg
    70921 atcgtgatat cacgcttttg ctgaaagcat ttgggacatt agaaggggtg atccgcgtcc
    70981 tcgatcctga agtttccctc atggaagtcg cttcgccttt tgctcaacat tattttctca
    71041 ctcatctgga tgtagaagat acattgaaac aaagcggatt agacttattg gaagggatga
    71101 aggctgcgcc taagatccct caacagctcc accatctatt agaaatgtgg acttcgggac
    71161 aaggaaaagt caatttggaa ttgaaaaagc aagacaaatt attgagcagg atcgaaagta
    71221 tgatcaatcg gcttgttttc ggtatgattt tagctgcatt gatcgtaggg tcctccctgc
    71281 ttgtccaagc agcacctgta gaaaacgcag aggtcgtttc cttattaggg atatttacgt
    71341 atgctattgc cgcattcgtc attatttttt tggcaatcga tgcattgatc caaatgtata
    71401 agaagaggaa aaagtagaaa aaggttgtga cagaaagaat cagcttcaag aaataagaag
    71461 gggttttaca taaattgttt ctcaaatttt tgtgaaaatt cggcttattt ttcgaagagg
    71521 ctacttctga aacaccgaga ataaaaaatg tgaaaaagct gttctgcacc cagtaataag
    71581 aacaatcaaa aagggaacat ggaacacatt tatcctgttt ccctgttccc tttttgatgt
    71641 attcgagcat aaataaaata ccttcgttac ctactatctt ctgtacttgg taaaccttag
    71701 tcaattgcct tttttaacgc ttgacgaaaa gacatatgat ctttctccgt aaagggtttt
    71761 ggaccctttg tccttatgcc agcttttctt aactttccac tatgataacg ttgttctaga
    71821 agcgtgccga tattcgcttc gtcaaattct tttccagagt gatggaacac tcgcacaccc
    71881 ttcggcaata caagtgaagc taaaccataa tcttgcgtga tcaaaaagtc attcttctta
    71941 accataccga cgattttata atccgcacta tcagctccgc ggtctacgta gacgatttgg
    72001 acatgattag cgtattcttt ggtcgtataa tggtccacac tggtcacgat aaccacgtca
    72061 agaccatagg attctcctaa ttttatgaca tcttctttta ctggagaacc atctccatca
    72121 atgaatagcc tcattctttt tcgccttctt ccataaatga taaacagtta ctaatccgct
    72181 cagtaaaaga ccaacattga tccattttgc caagataagc agttgtacac tttctatcgg
    72241 cactgattca tggttcagcg tgatcaataa tagatggatt ggctctccca tttcttttgc
    72301 taaaacaaac aaaagcgtca tcaataaaaa ctggtggatc acactccctt tattttgagt
    72361 agtatacttg acaacgatcc ggcgatccat tggaatagct gtatcagttt gataatattc
    72421 cttatttagc cagctgcctg ttactacgtg ccagaaacta agcagaaaga cacctatcgc
    72481 aaaaccattt ggaatcaaga ggaccaataa agaagaactc agtccaagca attgcatagc
    72541 caatggagac catgttaaac gtttcaaaat ccatggtccg actatttttg ctgcaatcaa
    72601 gcctgctgca taaggcaaat acataaaaat cgaaagtttt tctaatccgt acagtatgcc
    72661 aacataaaaa ctgctaaata aaaaaaggaa attccctagc attccattta gaaaagtaaa
    72721 cagatttagc cagcttggca acttccattc cttcttctgg tgtcctaaaa caaacacgaa
    72781 caagatgaac agcagcaaga aaccataaat agcaagatca aaaagccttt catcgaataa
    72841 caatcgggca tttctcaaaa gaaacaataa gccaaagaat atcaaaaata aaacaagctc
    72901 tcgcgtagct accagatttc ttttgatgtc tttgaaatcc agttcgtaac gcggataatg
    72961 actaaccgta tggtaggcca tgacgtaaaa aagtgtataa atagtaaagc ttgctgcaat
    73021 ctgagtcggt gcaggcaact caatcgcttg atacaggaga aatagccaga tagctgcaaa
    73081 taagtacttt ttcccatcca tattgataaa accttgcgtc ttttcgtgaa aattgaccgt
    73141 ggtattagca ggtacaagcc atgctgcact cagccctagc aaaacacttg aaaaataaaa
    73201 taaaggaaaa taaaatacgc ctaatattcc aagcaatgaa ccagcaccac caatcagcag
    73261 agcgatcatc agcaacgtgt aactatctag accaaatcgt agactgttga ctaaaaatag
    73321 tccagtcatc cgaaaggcat aaaacagcgt aaatggcaaa atcgtcagcc aattatgcga
    73381 gtctgttagt cccaaaaata aaagataagg aataaaaata accgtgttac ttaagataaa
    73441 gggcgttgtt tgtgtaaaat tacgcccaaa tcgtttgagt ttttcgaaca tgacaatggc
    73501 tccttaaaca gtcttataac taatctgctc ttcgatttta gcattaaagg ggaaataaac
    73561 caattttaca gaatatattc ttgtaaaaaa aaatacagaa agaagcataa gcttctttct
    73621 gcatttttac ggtacttgat tactcaagca gacaagccct tctccacaaa agtcttgccc
    73681 gacacccctg catccggcag gtgataaaga tagaaatcaa ttatcctgtg cctccaaaat
    73741 caattaggag ctctgtaaac cagagtcctt ctcgacccgt cgcataaatt tactttaaca
    73801 cagttcctat gtttatgcga gagctttttt cattttataa tcgaccattt gaaatccttg
    73861 accgatatct gaaataagtt cttctgtaat ctcaaatcct tcatgcttat aaacctcgat
    73921 tgcttgatga ttcccctgat tcactcgcaa aaagatctct ctttcctttg tttgcgcagc
    73981 aagttcttca taccagcgaa aaactgaaga agtcaatcct tgaccacgta ctttatttag
    74041 caaatataat ttacttaaat aaatttttcc atttatctct tcataagcag tgtaaccgac
    74101 agtcttttct tcgcttttta ataaaaagta ggacacacct tctgtcagtt ctttttgaat
    74161 ctgttttttt gactgataag ttttcagcat gtaatccact tgtttttgac caatgatcgg
    74221 cacaaaaact tccggccata cttcttgtat gactgccatc agctcatcca gttcattttc
    74281 tgttactttt attttttcca tacacccacg tcctttttta cattataaat gaccttgcgc
    74341 ttgatggaat aaaaagagca tgatcatgct atacacaccc accgaccacg gtttcaagaa
    74401 aatcacaatc tgcataaatt ccctaaaaga taatttcgtc aaaccagcta ctagacagat
    74461 caagtcgtct ggcgcaaagg gaagcagcaa ggtaacaact agtacttttt taatatcttt
    74521 cgtttttctt gtcagcaatt catcaaattt ctgatacttt tttggcgata aaataagctg
    74581 gacaaaggct acgccatagt aacggacaag caaaaatccg attgcttccc caatcactag
    74641 gccaatacaa ctatataaaa gcccaaatcc attgccaaac atcagcatgc ccgctactga
    74701 ggagatccct cccggcaaaa taggaatgat cacttggatc acttgcaaca gaacaaatat
    74761 catcactgca tacttgccaa attgctggat aaacgcttgc aaagacgtgc tcgatcgaaa
    74821 aatcccatga tgataagcat aaagaatcaa cacaaagaaa aaaaacagac caataatggg
    74881 catgatctga agacattttt gtaaaactga tcctttttta gacatctata ttccccacat
    74941 cttttttcat ttattaaaac tgcctcctt
//
LOCUS       DACDIG010000005        61237 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.005, whole genome shotgun sequence.
ACCESSION   DACDIG010000005 DACDIG010000000
VERSION     DACDIG010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61237
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.005"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(38..1189)
                     /locus_tag="IV247_000391"
     CDS             complement(38..1189)
                     /locus_tag="IV247_000391"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAP8776489.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1202..1639)
                     /locus_tag="IV247_000392"
     CDS             complement(1202..1639)
                     /locus_tag="IV247_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8776490.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1654..3420)
                     /locus_tag="IV247_000393"
     CDS             complement(1654..3420)
                     /locus_tag="IV247_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAP8776491.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3684..4361
                     /locus_tag="IV247_000394"
     CDS             3684..4361
                     /locus_tag="IV247_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8776492.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4364..5491
                     /locus_tag="IV247_000395"
     CDS             4364..5491
                     /locus_tag="IV247_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8776493.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5691..6131)
                     /locus_tag="IV247_000396"
     CDS             complement(5691..6131)
                     /locus_tag="IV247_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8776494.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6142..8175)
                     /locus_tag="IV247_000397"
     CDS             complement(6142..8175)
                     /locus_tag="IV247_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP8776495.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8177..9880)
                     /locus_tag="IV247_000398"
     CDS             complement(8177..9880)
                     /locus_tag="IV247_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8776496.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9974..10924)
                     /gene="manA"
                     /locus_tag="IV247_000399"
     CDS             complement(9974..10924)
                     /gene="manA"
                     /locus_tag="IV247_000399"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAP8776497.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10963..11988)
                     /locus_tag="IV247_000400"
     CDS             complement(10963..11988)
                     /locus_tag="IV247_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP8776498.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(12197..13066)
                     /locus_tag="IV247_000401"
     CDS             complement(12197..13066)
                     /locus_tag="IV247_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAP8776499.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13525..15417)
                     /locus_tag="IV247_000402"
     CDS             complement(13525..15417)
                     /locus_tag="IV247_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8776500.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15414..17141)
                     /locus_tag="IV247_000403"
     CDS             complement(15414..17141)
                     /locus_tag="IV247_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8776501.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17157..17807)
                     /locus_tag="IV247_000404"
     CDS             complement(17157..17807)
                     /locus_tag="IV247_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8776502.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18011..18307
                     /locus_tag="IV247_000405"
     CDS             18011..18307
                     /locus_tag="IV247_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP8776503.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            18479..19099
                     /locus_tag="IV247_000406"
     CDS             18479..19099
                     /locus_tag="IV247_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAP8776504.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19191..20357)
                     /locus_tag="IV247_000407"
     CDS             complement(19191..20357)
                     /locus_tag="IV247_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8776505.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20369..20722)
                     /locus_tag="IV247_000408"
     CDS             complement(20369..20722)
                     /locus_tag="IV247_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP8776506.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20850..21872
                     /locus_tag="IV247_000409"
     CDS             20850..21872
                     /locus_tag="IV247_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAP8776507.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(22311..22745)
                     /locus_tag="IV247_000410"
     CDS             complement(22311..22745)
                     /locus_tag="IV247_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8776508.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(22855..24714)
                     /locus_tag="IV247_000411"
     CDS             complement(22855..24714)
                     /locus_tag="IV247_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP8776509.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(24832..25992)
                     /locus_tag="IV247_000412"
     CDS             complement(24832..25992)
                     /locus_tag="IV247_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAP8776510.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(26299..27723)
                     /gene="araA"
                     /locus_tag="IV247_000413"
     CDS             complement(26299..27723)
                     /gene="araA"
                     /locus_tag="IV247_000413"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAP8776511.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(27739..28437)
                     /locus_tag="IV247_000414"
     CDS             complement(27739..28437)
                     /locus_tag="IV247_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP8776512.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(28394..30055)
                     /locus_tag="IV247_000415"
     CDS             complement(28394..30055)
                     /locus_tag="IV247_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAP8776513.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            30273..31673
                     /locus_tag="IV247_000416"
     CDS             30273..31673
                     /locus_tag="IV247_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAP8776514.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(31925..33004)
                     /locus_tag="IV247_000417"
     CDS             complement(31925..33004)
                     /locus_tag="IV247_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8776515.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(33090..34049)
                     /locus_tag="IV247_000418"
     CDS             complement(33090..34049)
                     /locus_tag="IV247_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAP8776516.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(34061..35584)
                     /locus_tag="IV247_000419"
     CDS             complement(34061..35584)
                     /locus_tag="IV247_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP8776517.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(35594..36157)
                     /locus_tag="IV247_000420"
     CDS             complement(35594..36157)
                     /locus_tag="IV247_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP8776518.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(36163..37026)
                     /locus_tag="IV247_000421"
     CDS             complement(36163..37026)
                     /locus_tag="IV247_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8776519.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(37040..37936)
                     /locus_tag="IV247_000422"
     CDS             complement(37040..37936)
                     /locus_tag="IV247_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8776520.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(38111..39382)
                     /locus_tag="IV247_000423"
     CDS             complement(38111..39382)
                     /locus_tag="IV247_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8776521.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(39372..40889)
                     /locus_tag="IV247_000424"
     CDS             complement(39372..40889)
                     /locus_tag="IV247_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP8776522.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            41087..41977
                     /locus_tag="IV247_000425"
     CDS             41087..41977
                     /locus_tag="IV247_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAP8776523.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(42050..42874)
                     /gene="nadE"
                     /locus_tag="IV247_000426"
     CDS             complement(42050..42874)
                     /gene="nadE"
                     /locus_tag="IV247_000426"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAP8776524.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(42886..44358)
                     /locus_tag="IV247_000427"
     CDS             complement(42886..44358)
                     /locus_tag="IV247_000427"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAP8776525.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(44995..45357)
                     /locus_tag="IV247_000428"
     CDS             complement(44995..45357)
                     /locus_tag="IV247_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP8776526.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            45580..46332
                     /locus_tag="IV247_000429"
     CDS             45580..46332
                     /locus_tag="IV247_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8776527.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(46472..47395)
                     /locus_tag="IV247_000430"
     CDS             complement(46472..47395)
                     /locus_tag="IV247_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP8776528.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(47410..47682)
                     /locus_tag="IV247_000431"
     CDS             complement(47410..47682)
                     /locus_tag="IV247_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8776529.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
     gene            complement(48152..49777)
                     /gene="groL"
                     /locus_tag="IV247_000432"
     CDS             complement(48152..49777)
                     /gene="groL"
                     /locus_tag="IV247_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAP8776530.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(49828..50112)
                     /locus_tag="IV247_000433"
     CDS             complement(49828..50112)
                     /locus_tag="IV247_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAP8776531.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            50337..50999
                     /locus_tag="IV247_000434"
     CDS             50337..50999
                     /locus_tag="IV247_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP8776532.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            complement(51075..52367)
                     /locus_tag="IV247_000435"
     CDS             complement(51075..52367)
                     /locus_tag="IV247_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8776533.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            52537..53166
                     /locus_tag="IV247_000436"
     CDS             52537..53166
                     /locus_tag="IV247_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HAP8776534.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            complement(53269..54078)
                     /locus_tag="IV247_000437"
     CDS             complement(53269..54078)
                     /locus_tag="IV247_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP8776535.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(54133..55002)
                     /locus_tag="IV247_000438"
     CDS             complement(54133..55002)
                     /locus_tag="IV247_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8776536.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            complement(55003..56319)
                     /locus_tag="IV247_000439"
     CDS             complement(55003..56319)
                     /locus_tag="IV247_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8776537.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            complement(56316..58151)
                     /gene="walK"
                     /locus_tag="IV247_000440"
     CDS             complement(56316..58151)
                     /gene="walK"
                     /locus_tag="IV247_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAP8776538.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            complement(58156..58860)
                     /locus_tag="IV247_000441"
     CDS             complement(58156..58860)
                     /locus_tag="IV247_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8776539.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            complement(59050..59910)
                     /locus_tag="IV247_000442"
     CDS             complement(59050..59910)
                     /locus_tag="IV247_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8776540.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            complement(59897..60466)
                     /locus_tag="IV247_000443"
     CDS             complement(59897..60466)
                     /locus_tag="IV247_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAP8776541.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            60847..60919
                     /locus_tag="IV247_000444"
     tRNA            60847..60919
                     /locus_tag="IV247_000444"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:60880..60882,aa:Lys,seq:ctt)
BASE COUNT        17703 a        12807 c        10557 g        20170 t
ORIGIN      
        1 aaaaatttcg tcacattccc ctttttcgta tcctatttta agcgacttgc ttggcataac
       61 ggttcacgtt ttgcttgata ttcccgattg ttttcggatc attgattcct gtgacttctt
      121 gaatcaattg ttccaagttt tcatggatct tcatttcatg caattgacgg ctttgttcat
      181 cttcaggatc atagtaatta aaaataatcc ccattgttgc cgtcaaatgt gggcaagtca
      241 agttatgttc ttgtagttct ctcactggac gtgtaaatct ttcttgataa cctaacttac
      301 gtaatggtgt ccgagcgaca cgtgtgatcg catcagagat atttttgttt tggaagcgat
      361 gaatgatttt ttcaatatac gcatgatgct gctctgcatc aaacccccat ttggcaatca
      421 gcaaacttcc tgtttcctgc aaaactgatt ttaattgtgc cacaactaac gcatcttgca
      481 ttgcttcatc aatggtctgg taacctaaca aggcacctgt ataagcgaca gtcgcatgac
      541 cagtattgac actaaatagt ttgcgttcga tatatggttc tagatcatct acatataaaa
      601 ccccttttaa ttggattttt ttattttttc tttggctatc gtcaatgacc cattctttga
      661 aaggttccac ttggacgaat aaaggatctt catggtgttg taatgggacg attcgatcga
      721 ctgctgcatt tggaaagccg atatatttgt cagtaaatga tgtttccgga agatatttat
      781 acacttcttt ttctagaaat gctgaaccgc caatcatatt ttcacaggca atgatatcta
      841 aagatgttgt gcttccttcc ttttctcttt cttggatacc ttttgcaatc aactcagcaa
      901 taaaaggtaa gatattcggg ccaatggcgg tagtaactaa atctgcttca gcgatttctt
      961 ggattacttt ttctggattt gtttgattgt tgattccttt gacatttttt acagtgattt
     1021 tttcttttga ttcatctgct aattcgattt cgtattcttt tctttcattt aaagcatcaa
     1081 tgatcgtgtg attcacatct acgaaagtaa tctcaaatcc attagctgcc aaaacctctc
     1141 caataaatcc tcgaccgata tttcctgcac caaaatgtac tgctctcatt tttctctctc
     1201 cttaagcaat tgctagattt tccgttactt cttctttgct gagcgcgtct gctagctgga
     1261 caacattgtc catatcactg caataaagtg cgatttgctg tacaagttgc aaatgctcat
     1321 ctccgactcc tgcaataccg aacaaaacag tcgcaatctt ttcttcttct tcggtaccaa
     1381 aatctacacc atctggtact tgaacgacac agatgccgga ttttttcaca tatttttttg
     1441 cttcatctgt tccatgagga atcgcgataa aattccccat ataaacagat aacattttgt
     1501 ctctatctac catggcatcg atatattcct catcaacgca ccctgcttct acaagcatct
     1561 gaccgcaata gcggatcgct tcttctttgg ttttaaaaga tcgattgagc tcgatcatgt
     1621 cgaatggcaa actttgcatg ctgttttcct cccttaagct gtctgtttca aatattgtgc
     1681 aatcgattcg tatttttgcg gattgaccat gctgttgact gataaatgaa tggattgagg
     1741 tgcagccatc tgtacttttt ccgctaaact ttctttggct acgatcagtg aatcaggttc
     1801 atcttttaaa tcttggatat tgacttttct taacgggata gttaaatggt tcttgtctaa
     1861 tatatctttg agcacggtca tccccattgt tgctgatccg gaacgatcat cgtggacaag
     1921 tagaatctct ttgatttgtt tgatgtttgc tgaaagaaac tcttctgctg gctcattctc
     1981 aatttcagaa actgatgttt tctgcttgct gcttaattct tgaatgattt catcatatcg
     2041 aggtgaattc aaaaagtttt ctactgagac aaaacgcgcg ctcggtgctt tttgttttgc
     2101 tctttcttgt agttcgacct gagtaacgat caatgtattc gagtcatctg tcagttggct
     2161 gattgcttga ttggtcaccg tttgaggcaa gtctgcttcc tgtacttttt ttctcaagat
     2221 cgaagcgccc atcgcacttg atcccattcc tgcatcacaa gcaaaaatga ttttttgtac
     2281 gccagagaat gtagctcctt gaggaatacc ttgggcacct ttggaagctt gcttagctgc
     2341 ctttgtctct tgaacagatt tttcgaagtc ttcatttgta tctttcgcat ccgctttcaa
     2401 aattactgca gaaataccaa acgaaacagc tgcggcaaca atcacgccca aaataacagg
     2461 aaggtaagaa ttgaacggtg tcatcgctag aatcgcaatg atcgaccctg gagaagctgg
     2521 cgctcttagt ccagcatcca tcaattggaa aatgaaactt cctgacattc ctccgcctat
     2581 aactgataag aataataacg gtttcatcat aacgtaagga aaatagattt catggatccc
     2641 gcctaaaaag tgaatgatca tggctcctgg tgctgaagat tttgctgctc ctttgccaaa
     2701 caaagtaaaa gcaagtaata cgccaagtcc tggaccagga tttgcttcaa gcaagaatag
     2761 aatagatttt ccagcctctg ctacttgttc agttcccaat ggtgtcaaaa tcccatgatt
     2821 gattgcatta ttcaaaaata agatttttgc tggttcaata aaaatacttg tcaatggaat
     2881 caaatgggca gataaaatga tctctacacc ttttgccatt gcttgagtca atgcatcgac
     2941 aactggacca atcgcagaaa aacctaataa cgctaatcca aaaccgataa gacctgctga
     3001 aaaattattg actagcattt caaaaccgga tttgatccgt ttttggaata gctgatcaaa
     3061 tttcttgatg gcatatccac caagaggtcc catgatcatc gcgcctagca tcattggaat
     3121 atctgttccg acaatcacac ccatcgttgc aattgcccca acaactgatc cgcggtcacc
     3181 agcaattact tttccgccag tataaccgat caataaagga atcagataag ttagcattgg
     3241 gcttaccatg gtagccaaag cctcatttgg taaataacca tctgggataa atagagccgt
     3301 taatacaccc caggcaatca gtgctccaat atttggcatg accatgcttg ataaagtact
     3361 gcctaatttt tgtaccctcg cttttagcga aatgttgttt ttgggaatcg tccgttccat
     3421 tttgtctctc cctctctcct tttgatactc taattgtatc cgcttactaa aaagagaaat
     3481 agtcgtactt tggcacgaaa ctttgtgcca aaggtttgag tatgtcagca aaataaaaag
     3541 atttcggtat cagaaaactt caattttaaa taaaactgtt attttaattt ttttataata
     3601 tgcaaacaat aacttttgtt agcatttaat ttagatgttt tccattctat tatgttatta
     3661 aattttatat tggaggccta tctatgacat tactgatcaa aaacatgaac atgaaacgtg
     3721 gtaaaaaacg tgtgattcga caattgaatc ttttagtaga atcaggagaa gtagctgctt
     3781 tgattgcccc gaacggattt ggcaaaacaa ctttgctgaa tggcattgcc caactgctgc
     3841 caacaactta tgatcaattg tcccttcaca acatctctcc tactgcttat acagattatc
     3901 gccggtcttt cttttttcta gaatcaacga atcagttatc cgactatcta tcggctgttg
     3961 attatttgac tttgatcaaa tactattggc agagcaaagt agaatgccaa actgttttcc
     4021 aacgattgaa gatgactgag taccttactt tgccgattgg aaaaatgtct ctaggaatga
     4081 aacaacatgt cttgcttgct gcttacttga tcagtgattc atctgtcatg ttattcgatg
     4141 aaccgttgaa cggattagac ccaggaagta ttgatatctt gaatagcctc atcagacaat
     4201 ggcagaaaga ggggaagacg attcttcttt cttcccacgc tatcgcgaat atccaatcga
     4261 tttgttctaa agcatgtttt ctaaaaaatg gagaaatcgt cacagaatcc tcaaatatgg
     4321 aagagatttt agctatttat cgtaaattat atgaggtgta actatgtctt tacttacatt
     4381 tgaacgcaat ctcttactaa aaaacaaagt aaacttttta tttcctctat tgttggtcgt
     4441 gctctttgca ttcccacttt tttttgatca taaacttgct tatacagaat tcgatgaact
     4501 gaatcataac tatgaagaga tgcaacgttt gattgaaacg ttgaaagaag atgagaatga
     4561 gaaagaattt gtggaaagtt tagagaaaag caacaaactg atcgaagcaa tcttacatgc
     4621 aaaaaacaca gggaatgtcc agcaaatggt cgaagcaact tatcattatg aaaaagatat
     4681 actggatcga ctgatttctg gtcaaagaca aggaattcca atcattgaac aacaaaaacg
     4741 tgtcgagttg ctccgttata tgaaagaaca tcagatccaa agatattcta tttttgactt
     4801 gccagcgcat ctccctttag caaattacta cgaaaatatt ttttcaggaa tgatttctag
     4861 ttttttgatt ttatgtataa ccgccttgtt tctttcttcc atcatctctt atgaaaaaag
     4921 aaagcaggta atctctctag tcaatctatt accggatagt atggtaaaga aacatagcat
     4981 ccgttttacc atttattatg gtgcagctat gctaagtctc gtgatgccat ttttgatcgt
     5041 ctctatacta gtaataatca aaaatggttt gggagacttc cgttatcctg taggaacgat
     5101 cattggtcag gagatacgaa tccttccgat gtatgagtat cttttccaga gctttctgtt
     5161 cctgttgttg tgggtgctct ttttaagtac aatcagtttt cttctatctg ctttatttga
     5221 acattctcta gtcaatcttt taggtacact tctttgtctt ttcttagcag agtatcgctt
     5281 gttttcttcg attggatgga tagaatcaat cagtcattac ttgccaactt cttatgttga
     5341 ttttcaaaat gtgatcatag gaggagatat tttctcgcca ctagcttcag aacaggttac
     5401 tttcatgaat ggtatcttga cacttggtat atggagtatc gttttattat tcataggtat
     5461 gggcactatc tatataaaaa agagctatta aaacacaaaa ataatagggg cggtgacaaa
     5521 aaccttgtca ccgtccccta tttcaaataa acggttttct agaataaata gttttcaaaa
     5581 gcttgcatcc gcagtaataa gaacctccca acaaaaatag ctcctcttat ttttatcttg
     5641 gttgaagtta ttacttgatg caaggcagct ttttttactg tgaacattgg ttatatatgt
     5701 ccatgttcaa aaatcatttg gacttcttca atggttttcg catcactgag cgccattact
     5761 tggtcgatgt ctgaacagaa aaaggctaaa tcttggagca ccgctaactg atgttctttt
     5821 tttaaagcca ctacaaataa gagtgtcgcg atttttggtt cctcttttgt tccaaagtcc
     5881 acaccatcag gcacttggac taaatagatt ccttcttcta ctacttcttc atctttgtca
     5941 gtcccatgtg gcaaggctac gaaattcccg atatagacac tcgctttttc ttgtctttcg
     6001 atcattgatt ggatatatgc cggtttgatt cccccttgtt gggtcatcca ctggccgatc
     6061 tcggtcaacg cttctttcca attttgatag gattgatcga gcaatatttt ttctgctggt
     6121 agctgtatca tcttttcacc ttcatttctt tatttcttca atcaggattt tcgctaacaa
     6181 ctgataaacg atttctttat ttcctgagcg gaagatctcg gtgttcaagt cattcatgat
     6241 gattgctcca ctgatctttc ctaaaagaat cccgtctttt tcttggattg gcgaaggcgc
     6301 cagcataact aacatacggg acaaatcgac tggctgtcta tccatccctt caatggtcaa
     6361 cggatgtttc aaattgaaaa tacaaaagat cggtttagaa atcaatgagc tggatgtatg
     6421 gaataacccc atatttgtat gaggaatacc gataggtgct tgttcatatc gcttaagcag
     6481 ccggcgatga accacctctt catccgttac tagtccttca ggaaaataag gaaataagct
     6541 gcgaatcgtt tcggaaatcg tctcaggatt atccagttct tggataaaaa aacgaccgag
     6601 taattgattg atttgatcca tgaaatgcat tgtttcctca taagtatctt ctatcatcca
     6661 ctcgttattg ctgttatacg taattttttg cctttttttc ttctcaatat gcgaaaaagc
     6721 ttcttttagc tgcttgactt catcttctag cagtagcggg gagactaaaa tatattttcc
     6781 accgtatcct ggtaaaatag atgtagaaat aacaacatca tattcttctt gtaaatcgat
     6841 tttttttaaa tcagcaatcc gatagaaatg aatttgttgg atgaagggaa aatacctttt
     6901 caatcgtagt tccaacatgc tagtagaagc taatccactt ggcgaaaatc ctgcaatact
     6961 gatattgctt tctttcggac ttcgttccaa tgaattcgca aaatgaagga ccatataagc
     7021 gatctcttct tcggataatt ttttttcttg aaaaatccgc tctactgctt tacggattgc
     7081 attcgcaact tcctcgtatt gaaccatgat tttttctaaa atcggattgg tcagtgtctc
     7141 ttcttgtaaa acggctcttg aaaacacacc agataaatga gtcaacagca ttttgtacaa
     7201 agtttcatct tcataaaaat ggacattcgt ctcttcactt acttcttgaa tcagttgttt
     7261 tactaaataa gcaagctgtg tatcgaacgt ctcttcaaag aaattctgat caaatgagtt
     7321 cgtgaagtca tttaataatc ctgcaaaaaa gacgatctca tttactggat aaagttgttt
     7381 tgtctcagaa gcgatccgtg aaaacaatcg tttagctgtc tgtagtaatt ctttgtttgc
     7441 cagactatta tatgtctcat ttgtcagtaa atgattttta gtcacacgct ccatcgtcaa
     7501 tgtcagcatc aaaaccaaat gttcaagctt tctatcactt agattggtct ttttttggat
     7561 caattcttcc ccgattactt tgcgtgcaag agatagatgt tgcggatcaa cgaatcgaaa
     7621 aaaataattc agtgatgcat cgaaagaaat actatgaaaa agttcatatt cattgatttc
     7681 taattctaat acattcgctg tcagaagccg gcggtatttt tccggtcctt cgatctcgta
     7741 tccctggttc cgaatgattt ccaaaggcag ttggcgaata ctttcttcca actgtttgat
     7801 atctgcataa aaagtggtat tactgatgcg atatgattcc atgaaatgag caagactcaa
     7861 cggctctgtc gtcagtaaca actcggctaa aatcgcgtgt tgtcgtttag cagtggataa
     7921 gtcattactt ttcccatctg tattttcttt taattgctct aatacttctt ctgatgcctc
     7981 aatgacaaat gagcctctag ctgctttttt caaagaagca tttaccgatt tcaatgagac
     8041 ttcaagatta tctaattcgc ggtaaatagt ccgtttactg accttcaata aattcatcat
     8101 tttttggata gatacaggtg acgggctact gagaaattct gctaataatt gtttttctct
     8161 gggcgataaa tacatcttat atttctccct tcacgagttt ttcgtattca ttcgtagcta
     8221 cccaatgttc aactagaact agccgaggga tcttttcttc tagctgttca gttacagcta
     8281 gttctctttg tgcgatataa atcgtttctt cttggatttt gattttttct attgattctt
     8341 ttgagataac aaactgtttt ttcgcttttt ttgctaattg acttaaaatc gtcatgccca
     8401 tagtttgtga gccgcgtctg ttgtttttat acaaaaatac aatattagca ggcgactttt
     8461 tttgttctgt gatatctgct atctcttttt tttcttcccg ctttggaaat tggatcgctt
     8521 cttttattcg tgacagcaag atatctgttt ccaaaaagtg atccagtaca tagatttgac
     8581 tttgaggtac tttttctctt gcaagacccg ctagttcatg ttgcacgata accagctgat
     8641 ttacttggtt attcaattca tagatagagc tgtatccgac agaaagatcg attttttctg
     8701 ctgctaattt ctttttcagc agggcggctc ccatagcact tgatcccata cctgagtcgc
     8761 aagcaaaaat gacggaatga atgatagatg gttccaagat ttctcccctt tctttgaccg
     8821 ttttgttctc tttttcttta tttggtaatt tcaatagtat cgctgcacaa atgaaggtca
     8881 caaatgtact gattgctatc cctatactta ccccagctag ttgtggataa ggcgcattgg
     8941 caaaaatcac gagtagtgac ccaggcgata caggtgcagc taatcccgta tctaaccatt
     9001 gaaatatcaa cgtgccgctt gccccaccag ccattacagc taaaaataga tacggtcgca
     9061 gcaagacaaa tggaaaatag atttcgtgaa tcccgccaat cgcatggatc atcaatgctc
     9121 cactcgcatt tactttttcc tgtttttctg caaaacaaag gatagaaagt aagatcccta
     9181 aaccaggtcc aggatttgcc tccatcaaaa ataaggaaga gtacccgact gtttgtgaga
     9241 attcgattcc taaaggtgtc aatacaccat gattgattgc attattgaaa aatagtactt
     9301 ttagaggttc taagatcaga ctgacaagtg gtaacagatt cattgtgact agccaagaca
     9361 caacggaggc gagcaacaag tgaacactat ttaaaactgg ttcaataaaa acgattccaa
     9421 ttatacaaat aacgctacca gtcagtccta ctagtaggtt gctgacaagc atttcatatc
     9481 cagccttgaa tttcacgtgg aatctagaaa caaattgttc atatacgatg ccggcaagcg
     9541 gcccgataat catcgctcct acgatttgtg gagcattgga acgagcgatc aaccctaaag
     9601 aagcaattgc accggcgact gccccttttt gtccccctac cgatttcccg cctgaataac
     9661 tgatcaagat gggtaaaagc agctggatca tccaatggca tacctcttct aatgtggtgt
     9721 gcaaatcgcc agacacacct tttgttgcta atgacaagat tccccaagcg ataaacagac
     9781 ttagatttgg catgatcatt tcatttaagt gatgtccagc ttttctcagt tgtttcatag
     9841 acacgcgact gctgttgttt gctttccatt tgtattccat ctttttcacc atacttctat
     9901 ttattgatgg acggatttta tgtagaaaaa gcagcaaacg aaaaaccgtc tgctgctttc
     9961 agctatctgg gaactatttg cctggttcag tcgcaatgat tgctagttca ccttggaccg
    10021 cccactcaga aacgccattt ggaataatac agcttgttcc tttttctaat ggatatgttt
    10081 ttccgtcaac gacaaggtct cctgctcctt cgattaccgt catcaatgta taaggggctt
    10141 gttttttgaa agatgtgatt cctttaatat cccactcata tacattgaaa aactcagttt
    10201 ctaaataagt gacgatcgat gaattccctt ttcggacttc tttgatttgt aattctggtg
    10261 ttttcgcagg gacagttgtt acatcgatcg attgttggat atgcagctct cttgtttgtc
    10321 cttgcgcatc ttttctgtcg tagtcataca cgcggtaagt cgtgtcagaa ctttgctgtg
    10381 tttctaaaat catgattcct ttaccaatag catggatcgt tccgcttggt acataaaaga
    10441 aatcaccttt tttaactggt actttcttca aaaggtcatc ccagcgccca tctttgatca
    10501 tctcagctag ttcttctctt gtctttgcat gatgaccata gatgatttct gcaccaggtt
    10561 cagcatcgat gatataccag cattcagttt ttcctaattc accttcatgt ttcatcccat
    10621 aggcatcatc tggatgtact tgtacagata gatcatcttc tgcgtctaag atcttgatca
    10681 ataatggaaa cactggttct gaaggatggc caaacaattc cttgtgttct gaccatagtt
    10741 catctaatct tttccctttg aattcgccat tttctactac actgacacca tgaggatgcg
    10801 cactgatcgc ccaatcttct ccgattttat cgttagggat atcaaaacca aagactgaac
    10861 gtaaacgact tcctccccaa attttttctt gaaaaactgg ttttaaaaac ataggttcct
    10921 gcataattca aactcctctc atttttcata gctattatag cattatttcg gtaataccgc
    10981 tttctctttc aaaaagtttt caacaatttc atctcgacat atgagatact ccgcacgatg
    11041 atcgacagga tgtacgcgat ttgacaaaaa tatcaacgct tcttgtgttt ttatatcaat
    11101 caagaaaaac gtacctgtat aaccagtatg aaaaagaatc gcatgaccgt cttctacatc
    11161 cttttttaag tcccatccta aagaacgata gccatttttt ttcggtgttt gatcttgcag
    11221 taaagaatca atcgtctctt cttctaaaaa acgaccatta cggatttttc ccttgttcaa
    11281 atacatttgg acaaaagtaa tgctgtcttt caaatcaata aagagacccg cgttacctgc
    11341 atgttcagcc aaaactcttg ctttcggatc atgagtttgt ccacgcaaaa cctcacccga
    11401 aggcagttgt tctgtgggaa cgattttttt ggaaggaaaa ggaagaaata agctgttcat
    11461 cattcctaat ggttccaata cttgttcttg aaagatttcc actgcagttt cttggtaaat
    11521 ttcctctagc atcaacccaa gaagaatcgt accggcatct gtatatttta cttcttttcc
    11581 tagttgctca cctggttcta gcttcaaata agcggcgatc aattcttcct gagatagctt
    11641 atctctgttt ggtatccatg ttttgatatc agaagtatgt gtcagtaaat gacgcaaagt
    11701 gattgtctga ttttcaaatt caggaagata tttatgaagc ggttgatcga gttctatctt
    11761 tccatcttct aatagacgta aaactaatga agtggttgca acgactttcg tcagtgaggc
    11821 tgcatcaaac aacagatctt ctgtcatcag ctgcttttca ggaacgactt gggcaaagcc
    11881 ggctgtaaat gtttctactt gattttcttt tatgaaggcg actgctacac ctggataaaa
    11941 tccttcttcc atctttttca aaataagctg tttcgtgtgt ggatacatct ttttctcctg
    12001 cttttctatc agattcttac agtccaagct taaagcttaa tctatgagga atcaaccttt
    12061 tttacacaaa aaaactgcgc gaaaaaccgc gcagtttccc ccggtatact attttctctt
    12121 tctatctcta gaataagcga tagtatgacc gttcataaaa tgacgagaca tgcgacaatc
    12181 gttaagaaag aaaaagttaa gcgtcgcatc gaatgtcagt tttattcttt tcttttggct
    12241 cactgacgaa ccaccattcg attcctatag catcatagat tgtgagtact gtgtgctttt
    12301 tgtctaagaa gaaatcaact gatagttgct gcaagtgagc aatcagtcct tccatgcttt
    12361 cagtatcgat catgaatttc agaaaatcaa gtccaatcac ttcatctgta ggcaaatcaa
    12421 ttgcgcctcc gctagcttct gtcaaaccaa catggaaatc tccttcattc aatacatgga
    12481 tgccctcttc ttcattatgt accttcaaac caagtacatg attaagaaat tggcgttctt
    12541 ttgttaagtt agtaacattt aaatggattt tttcaaaata agaacctgtt gatagtttac
    12601 tgaatttttc ttcagatttt cttaataatg cttcttgatc cattgtttct ggaatttctc
    12661 tttggtcgtt cttattctct tcatggtaaa tttccaattc attctcttct gggtctacga
    12721 gcaaaatccc catacgtcca tgatcttcta aagcttcttt gattggataa ttcattttac
    12781 ggatacgaca aaggatatct gccatttctt ctgtgctagg gataatcaat gaaaaacggt
    12841 tcagcttttt gatcaccccc atgtgatcat tcgctctagg actttcttcc agcagcaaca
    12901 tttcacttcc agcttcttct gttcctaaaa tggctaattc gttttcttct cttttcaaat
    12961 caaagccaac aacgttcttg taaaaatcaa tcattttatc tcggtctttc acccgaatgg
    13021 ctacactttt taaataggtg gcagccccca attgaaaatc tttcatattt cttgctcccc
    13081 tctacgtcaa gctttatttt aacgatggat tcttcttctt tcaagcaata actttgattt
    13141 tgttaaaaaa aatgtgagaa aattcgcatt ttgcttgttt tttgtaaatt aatgagtaaa
    13201 aactcttata aaaagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    13261 acaaaaatag ctcctcctat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt
    13321 tgaacaccgt ttaaggaaga ggttgtaaaa tcagcggttt gacctgagta ttagagatac
    13381 aaaacggaaa aatagcttct catattttgc cgttttttga gctaataccg aaggacagct
    13441 gattgaacac cgtttattcg gaaaaaggga ctgtgacacg acttttgtca cagcccctaa
    13501 aaaaaataat aaatttgtac tattctatga agcttcttct tcagaaaact gactgttata
    13561 aagatctgca tagaatccgt ctttttccat caatgtatcg tggtttcctg tttctacgat
    13621 cgaaccatga ttcatcacaa taatgttgtc tgcatcgcgt attgtagaca agcggtgagc
    13681 aacaacgaaa cttgtccggt tttccaatag gcggttcatc gctttttgaa tcagtatttc
    13741 tgttcgtgta tctacactag atgttgcttc atcaaggatc aggacatctg gatttgctaa
    13801 gaatgcccgc gcaattgtga ttagctgacg ctgcccttgc gaaatattgc ttgcatcctc
    13861 gtttaataca gtgttatatc cttctggcaa tttacgaaca aaatcgtcta cgtgagcagc
    13921 tttagcagcc tcaatgattt gttcgtctgt tgcatcgtcg ttcccatatt tgatattgtc
    13981 atagatactt cctgtaaaca gccaagtatc ttgcagtacc attgaaaact gtgcgcgcaa
    14041 ttcatcacgt gaaagatcac gggtatctac accatcgtaa cgaatactac ctccagaaac
    14101 atcgtagaac cgttcaagaa ggttgatcag tgtcgtcttc cctgcaccag tcggacctac
    14161 aatagcgacc atctcaccag gtttgacatc caagttaaaa tcagtcatta gcaagttatc
    14221 tggtgtgtag ccgaattgaa cattttcaaa acgaactttg tatggtgtat ctttttcttc
    14281 tggaaggcca gatttttcat tggacatctc ttcttcatcc aacacttcaa atacccgttc
    14341 ggctgaagca actgttgatt ggatagtgtt catcaaactt gcaagttgag tgattggttg
    14401 agagaactgt gttgtatatt gcaagaacgc ttgaacatcc cctaatgtca tgttcccatt
    14461 tgcaactttt actccgccta gtacagcgac gaacacatag cctagatttt taacgaaatt
    14521 catcaacggc atgatgattg ccgaaataaa ttgagctttc caaccagcgg catacagatt
    14581 ctgattttct ttttcaaata cttcctggtc tttttcagta tgattgaatg ttttaacaat
    14641 ggtatgacca ccatatgttt cttctacttg gttattcaat aaacctaaac ttttttgctg
    14701 atctgcgaaa aacacttgtg atttcggcgc tacgatcatc acgatgatca agcttaaagg
    14761 gatcatcaat aacgcgacta acgtcaattg ccagctgatt gaaagcatca tcacgatgac
    14821 cccaatcaat gtgactgcac tagttacaaa ttgagtcaaa ttttgctgca gcgtgcttgc
    14881 gatgttatcc atatcattga ttgctcgaga catgatatcc ccatttgtat gtgtatcata
    14941 ataggaaatc ggtacacgat tcattttggc ttccaaatca tgacgtaatt cataaactgt
    15001 tcgttgggaa acacgtgtca taataaattg ctgtaaaaag ttgaatacag cagaaatcaa
    15061 atacatcacg attactgtcg caatgatctg cgcgatttta tcaaagtcta tcggaaattt
    15121 tgttacttgt tggccttgtt gcatctgttg agcgccagcc attacacctt tgaatatttc
    15181 tgtggttgct ttccccaaga cctttggtgt ttggatttgg aagatcacgg ctgctattgc
    15241 tagtacaaat acagcgatga tcgcaaccat acgtttagac atatagccaa acaatcggcg
    15301 taccgttccc cagaaatttt taggcttttc accttttaca ccgagatttc tgccaggtcc
    15361 accatgtcct ggaccgcctg ctggtccatg agagggacgt gtgtttgttt tactcatgct
    15421 aagtcctcct ctcttagttg tgaatgaacg atttcttgat aagtttcgtt tgttgctaat
    15481 agttcttcat gtgttccttt gccaaccagt tttccttcat ctagcacaag gatcatatct
    15541 gcatccatta ctgtacttac tcgttgagca actaaaacgg tgatactttc tttcatattc
    15601 gttttcaatg cttggcgaag attggcatcc gttttgaaat ccaacgctga gaaggaatca
    15661 tcaaacacat agatatctgc tgttttgact aatgcccgtg caattgccag acgctgtctt
    15721 tgtccaccag agaagtttcc tcctccttgt tccacatggc tatctaagcc atcttccatc
    15781 tctgaaacaa aatctttagc ttgtgcgatt tctaatgctt tccaaatcat ttcatctgtt
    15841 gcatccggct taccatactg catattgtct cggatcgttc cagagaacag aactgctttt
    15901 tgaggaacaa agcccattaa ttctcgcaaa ttatattgag tcatgtcact aatgtccgtt
    15961 ccatttattt ggatcgaacc agattcgatg tcatataatc gagggatcaa attgactaag
    16021 gtcgttttac ctgaaccagt cccccctatg attgcgacga tctcgcccga cttggcttgg
    16081 aaatcaatat cttccaatgc aagtttttct gctccatgat atcgataatt gacatgattg
    16141 aaggccaatg ttgcgttttt tccagcaaaa gaaactgttt taggattttc agggtcatgg
    16201 ataccgattt tttcatccaa cacttcatta atacggtcag ctgaagcttg tgcacgaggc
    16261 accatgataa aaatagcgga aagcatcata aagctgatca agatctgcat cgcataagtc
    16321 ataaatgcaa tcagattacc gacttccaaa gtcatatctg ctatgtagtg tccaccaaac
    16381 caagtgattg ctacgtttgt accgctcatg atcaatgtca tcattggcat catcaatgcg
    16441 acgatcgtat taactttgat tgccgtattt gtataatctt tattcgctaa atcaaaacga
    16501 ttttcttcaa agcgtgtctt atcgaatgct cgaatcactc tgacccctgt caatccttct
    16561 cgaaatacca ggtttaaacg gtcagttttc ttttgcatac ttttgaataa tggaacagca
    16621 aagtacatga ttccgccgat caaaataatc attactggga tgacaactaa gaagattttt
    16681 gtcaactgat gatctttttg ataagcaagt atgctcgcgc caatcaacgt gattggtgca
    16741 ttgatcatca tacgtaaaaa catttgtgta accatttgga tttgagtcac atcattggtc
    16801 gttcttgtga tcaaagaggc cgttccatat ttatcaaacg catcttttgt cagactctct
    16861 gattttttat agacatccgt acgcaattgc ttacccagtt tctgtgattc acgagtagca
    16921 aaaaatgtat ttgcgatagc agctaagata ctgatcaaag aaaagccgat catcacaaaa
    16981 cctgtatgcc aaatataatc gacatctcct tgcgctaccc ctttatcaat aatattggag
    17041 gtaagtgtcg gcaaatataa atctgccagt acttggatca ccatgaaaac tgcagctgca
    17101 attgctgaag tcaaggagat ccgttttacg atttttagca tctagttccc ttctttctat
    17161 ttatatactc cgtttttcag ccaagaaagt ttcaaattga attcactgat tgcttgctct
    17221 actgagtaat cttcagatgt tgaaaataag cgatacgaat gcgcaattgt tgtaaataca
    17281 gtattcatca ctaattggat cagtaattcc aattcatgat cattttttac tttcagcttc
    17341 gtccgatcga tcaattcaag caactctttt gcatcttgtt tgtgttcttt atgtggatcg
    17401 tgaggcgttt tttggcgact cgtatgagga gcgacttttc ttgaggcacg gtaatccatg
    17461 ttcataaaca agttacgata aaaagaagca ttctcgcctt ccaaaatctc ataaatcatt
    17521 ttagaaaaat aggattcgaa tcctttgaat agatcaccat cggattcttt cattagttgt
    17581 aacatatctt tagagctgtc acgtctcatt gtctcaaagt aataaaaata aagatcctct
    17641 ttatcttcaa aatactgata gaaactgcct ctagggatat ccgctaattt gacgatatta
    17701 gcaatcgaag cttcttttaa gggtactcta gaaaactcga ttctcgcagc ctcgattagt
    17761 cttttttgtt tttctttagg caaatggaaa aatgtctgtg tcggcatatg gatcctcctt
    17821 tctttcaaga tgacacattg tcatatgacg ctgtgtcatt ataacacctc ctacctgttt
    17881 tgcaagacat ttgcatagtt tttttcatta tcatccgata cattaaaaaa gtgataagag
    17941 gaatccttcc atttgaatta atgagagttg cccattaaaa tagccacata ctaaacaaag
    18001 gatgagataa atggcccaat caattagtgt ccaagacttc aataaattgc ctctcgatgc
    18061 tgacagcatc gttttagata caagaaatac cttagattac acaaaaaacc acttaagcca
    18121 ctcgctttct atccccgctg ctatcttgcc aagacaatta aaaaatctag atccaaagaa
    18181 actttattat attctaagct atacaggcag gcgatcagaa gcattagccg atcaacttac
    18241 tgctaatggt tttcgtgctg tttatatcat tggtgggatg gaagctcttc agtcgagtgt
    18301 tgcttgaata ttgcttgtta gattcaaaag aggctgtgac aaaaaatcgt gtcacagcct
    18361 cttttatacc ctgtcttttt ttagttattt attcttcaac cttatcaata tgacttgata
    18421 gccttttctt tttctcatag ttataacaat catagcaata aaaggagaag gtaagcatat
    18481 ggcatctgtt ttagtagtca aaggtcatcc cctaactgca gaagaatcgc gcacagttaa
    18541 ggcattgacg tcttttttaa caagttataa agaaaatcat ccagatgatg aagtgactgt
    18601 tttggaactt tatcgcgatg atattccgga aatcgatgaa gaattattat caggttggga
    18661 agcattacgt gctggtgcag aattcacttc cttatcagaa agtcaacaac aaaaaattgc
    18721 tcgtttcaat gaattgactg atcagttcct tgctgctgat aaaatcgtta tcgcaaacgc
    18781 cttatggaat ctaaatatcc caacaaaatt aaaagcttgg ttcgataccg tcaatgtagc
    18841 tggaaaaacg ttccgctata cagaaaacgg gccagaagga ttagtgacag gtaaaaaagc
    18901 actgcacatc caatctaatg gcggtgtata caacggacaa gactttgcct ctcaatatgt
    18961 aaaaggtatt ttaaactttg taggcatcga tcaagtagat cagctattca tcgaaggaat
    19021 cgattatgac cctgaccgtg cagatgagtt gatgcaaaat gcattagata aagctgctgc
    19081 tttaggtaaa tcattttaaa aattgagata aatgcataca aaagagacag aggaaaaaat
    19141 cttcaaagca gcatttgtac cttggatatt ttcccatctg tctctttttg ttattctttc
    19201 tgctttttca acggattttc ttcataaaga gaagagattg ttgtgtacca ttcaaaaaca
    19261 tgagtaatca ctactttagc tgctgcataa acaggaatcc ccaagatcac accgatgatt
    19321 ccgaacaatt tcccagaagt cagcagaact aataaaatcg ttactggatg gattgctaac
    19381 tgattcccta gtactaatgg agagatcaag cgtccttcta ttgtctgctc tactgcaaaa
    19441 acgatcagta ctttgatgat catgaatgga cctgctacaa tggctaaaaa gatagccgga
    19501 atcatcgcta aaaatgatcc taagtaaggg atcaggttca aaaaacccgc aataatcccc
    19561 aacgtgacag catactctaa accaatgacc gcaaaaccaa tcataaacat gattgccaca
    19621 gcaaaagcca cagttagttg tcctcttata taagaagaaa cttgactatt catttcactc
    19681 agcacattca acgtcggttt ttgcatgcgt gtcggtaaaa atttgacaaa ataaggtgct
    19741 aattgtttac cgtctttcaa tagataaaat aagataaacg gcatcgtcag cagagccact
    19801 aaaatagtcg ctaccgcgcc gacaaaactt ccaagactct gaactgtact cttagatata
    19861 tcttggatca tatcgcctgc cgaattcatg aacttatcac ctgctgtttc caactgttcg
    19921 cgaaattggt tgaacagcgg gtcccgcaaa atctcggtta atttattatc aatcgtatct
    19981 acatattgag ggaagttact gatgaagctc actgtttgtt cctgaatctt agggatgatc
    20041 actacgctcc cccatgcgat tagtgcaaca accaaaacaa acagcccaat gatactgtaa
    20101 agccttggta cttttcgctt ttcaaagtaa tcaactaccg gattcatcaa gtaatacaag
    20161 attcctgcta aaatgatagg cagacctacg attgctaaaa attgccagat tggtgcaaac
    20221 aaatatgaaa cttttgtaaa taaaaagacg atcagcaata ttaacaatac aacaagtaag
    20281 gctgtgacta cttgattatt taaaaaccag cgccaaaacc acgataccgg tctgttcttc
    20341 ttattttttc cttccataat tcctcctgtt agttataaaa aatttggctt cccaaaccaa
    20401 tagcaggcag tctatgcgga tgaagataga ttccatttgc aatttgtcct tcttctggac
    20461 aagcatcaaa aatcaatgat acgtgagcta ctgtctctaa attcccattg gccatcggac
    20521 cgacataatc gatcgtatat acctgatcat caaaagcaat tgttccgcct gctttcaaag
    20581 aaaaaggttc gtcttttgat acttcttgaa tcaccgaata ttttcgtaag gcagctgttg
    20641 ctgttttatc gaacaaaatc agcatgggtt cttccttgtt tattgcttct gtcccaattt
    20701 ctgtaatctt tgcctgaatc attgcactct ctcctttaat tgctttcaaa aacagtataa
    20761 cacattctca ttctttagcc gtgtggaata attatgtgat agaataaaaa cgaaaactat
    20821 ttcattacaa ttaggaggaa tcagctcata tgatcgaaaa gataatttta ggaacctata
    20881 cgcgtcgcga aagtaaagga atctatacga tcgaccttga tacagaaaaa gaagaattag
    20941 ccaacctttc attagctgtt gaagaaaata gccctaccta tgtagcaaaa agtaaagctg
    21001 gcaatcttta cacagttact agtgtggatg gattaggtgg tgccggtgcg tacgataagg
    21061 attttcattt tttgaatgcg gtaacagagt ctggcgctcc tttgtgttat gtagcggttg
    21121 atgaagcacg tcaattacta tacggggcga actatcataa aggtgaagta aatgtctacc
    21181 atatattagg agatggcgaa ctgaaagcag cagattcaat attccatcaa gaagcgacag
    21241 gtcctcataa aaaccaagat catgcgcatg tccactatac ggatctgact cctgaccaac
    21301 gattagtcgt atgtgatctt ggtacagatc gtgtgtatac ttatgatgtg tccgaaaacg
    21361 gcaaattgaa tcttgtaact accttcactg cagagccagg aacaggccct cgccatcttg
    21421 tctttcatcc aaatggacaa ttcgcttatt tatttggtga attagacagc acagtccgcg
    21481 tgctttctta taataaagaa acaggtgctt ttgaagaatt acagaaaata tcgaccttgc
    21541 cagaagaatt cgacggtgaa aatggagggg cagctatccg catttcaaat gatggcaagt
    21601 tcctttatgc ttctaaccga ggacataact cgattgctgt attcgcagtt tttgaagatg
    21661 gttcccacat tcaaaacaag caaatcatct caacagaagg ggattttccc cgcgatttcg
    21721 cactagatcc aagcaataat tttgtcgtgt gtgccaatca gaacaccgat aatctgacat
    21781 tatatcgccg tcatcctgaa acaggattac tggaattact gaaaaaagat gtctatgtac
    21841 cagaatgtgt ctgtgtttat tttgatcgtt agtaagaaaa ggagaatgaa atgaatcagc
    21901 aagacgtatt agacagacta cgggaagaac taaatattcc tttctttgat gggaaaatcg
    21961 aagataaaga gtatagcgaa gaggaatatc agaaactcaa agccgattta cagaactact
    22021 ttgaacaata tgtacagaat gtagaaaact aatcagcttt ccatactatc tgaaaagaac
    22081 atcaggacaa gacttttccg tctatcctga tgttcttttc tacaaataaa aaaccgccga
    22141 gatggttaga ctcgacggaa aaggagtatt tactcgattt tgagtaagta caaggttgtt
    22201 gttggttata ggtatgtcat tatcataaag gattttgatc actctatcat aaaggtaata
    22261 ttacatttat attagataac tctagttttt attgttatca aagatttctt ttattttttc
    22321 aacaacaaat tagtcatttt ttggatggca gctgtcaatt ggtctgtcgc ttggtcttgt
    22381 tttgcttcag atgtattgaa tagatcttca tctacccctg attcaagatc gatactttgg
    22441 atcatttctt cacagcctgc tacatatgaa agataagctt tttcaaaatt tttatgaatg
    22501 cccattgctt ttgctggtgg acgcaaagta ctgactttct taagcattgc ccgatatttt
    22561 tttgttcctt cttcaaataa caggatgatc tcttcaagag tcgatttgtc cagttcactt
    22621 gtttttcctt catcaattgc gtttctgatc acttcatatt tcggatgcat tccttctccg
    22681 attgcttctg tttccttgac gatttgatcg atggactgca cataatagcc aatattcggt
    22741 aacatgttcg ttttcctact ttctccattg ttgttattat ttattctact tgtttttatc
    22801 ctcaaatgac aagtacttga aaaaatttaa tggtttctat acagctcgcc attcttattc
    22861 gtgctcaaag aaaccatctg ctaaactata ttgtttccct gcgacaatgt catattggat
    22921 caattgatat tcgtcgtaaa tctcttgttg cgagcgattg aattgcgctt ctttatacca
    22981 ctgtccgttt tgatagtaca gttctcgttc aaacgccgga agttcctgtt ctaatgataa
    23041 aagcagctga taaaagccat ttgttgtctg attcgtctgt tccattagat tcgctgcaaa
    23101 ataaaacgga ctggtgattg catcgtgagt cgtttgcttt tctaattctc ctttactgtc
    23161 gaacataaga aattgtgttt catgcaatgt ttgtttgtca ttttttgctt ggatcgtatc
    23221 tgaatagatc cctggaagat gatcccccca aaaaacaact aaagtccttc tagaaagatt
    23281 cttcaattct tccgtgaact gcttcagtgc cgtgctgcta taggaaatat cttgcagata
    23341 attctctaag tcaagtgtcc cacttccatc tgaagttttt gcaggatagt ctagattatc
    23401 atactttcca tcgtagggca tatgtgtctg catcgtgact aaatgaatga actgaggtgt
    23461 tttttcttcc ttgagaagtg tcattacttc tttgtaagct gactcatcag aaatatatgg
    23521 attattctct atcgtatccg tataggtcat cgtacgctcg ctgatgaact ggtcaaatcc
    23581 taatgtctga taaacatcct ctcttttata catcgaagta ttgtatggat ggatcgctgt
    23641 cgtctggtat gattgtgcgt ttaacgctga aacgatagaa ggaagctgat ccatttttgg
    23701 tacaagcatc gtgtaagggg tggtgagttg cgcattaaat aacgccatag aaaaaccagt
    23761 caatgcttca aattcgatat ttgctgttcc accgccatag ttttgagaaa gcatattgcc
    23821 actatatgtt tgatcagcta cttcataata atctgccaat ggctcaccag atacttctac
    23881 gccgtttaat cgactaggat ctgagaaact ttcactcatc acgaacacga tgttcggcga
    23941 ctcttcctct acagttttat ttttttcatc ggctagcttc tggtattttt cagtgatttc
    24001 tttgatcttt tctttcgaat atccttcagg ttcatccatt ggttctacct tcagattgta
    24061 caaaaaccca ccgataaaac cagtgttata gtagttcatt ttctggctgt aaggtatcca
    24121 tagtgccgtt ttattatatg cttttcgcaa caaattatcg ggattgttga aattactgat
    24181 ataccctagc agtccaattg ccacaagcag tgtcgttaat cggatcaatt ggaatcgcct
    24241 tccttttttc aggcttcgaa acatgtacca gcaaaacaat cctatcacgg ttcctgcggc
    24301 agctaagatc actgtaaaca gcatagtgcc ggtcatatct ttcagcaatc cgatttcagt
    24361 gatcatcttc aaatcgtctg gataaatcgg ttcttgccga taactcattt tcatataatt
    24421 tgcaaaacct aatacaccga tggctacgac atacgtaagg gaacctaaag ggacagatcc
    24481 tgctagtgca atcagaaaaa taagaataat cagcaagacg agacaggcta agaagaattt
    24541 ttccgtatgc caagaaaaag caaatttcaa cgcaaggtct acggatagat cattttgaca
    24601 ccactgcaaa aataaattac taaaaacaac aagaaaaaag atactgatga ttgctgctgt
    24661 tccttttaaa atctttttct gtttggtcga aagtctgctt atatatcttt tcaaatgctt
    24721 gctccttcat ttctatcaat aaaagaatgt tgcttctctt attttagttg tacgaaattc
    24781 gaaagtaaag aacggaaaga agaattaatg aaatccaaat attctctttc tttatggcct
    24841 ttctggtttc tttaataagc ggacctttcg tactccagaa attttttcta gctgttgttt
    24901 cacttgattg agctgttcaa aatcagtact gtctatgtca atcaatatgt agacatgctc
    24961 attcttatgg ttacgcgata tatctgcgat cgctagctcg ttctcattca gcagagaaaa
    25021 tatctctgct aatatattgg ttttcttttg ataaaatata gtgaatcgaa acggcgcttg
    25081 gaaaaacatt cgtgcggatg gataattgac cgcttctcta accgtaccga acaaaagata
    25141 atcttttaag gcttcaactg ccaaacgtcc gctgtctgcc aaagcttcct gagtcgatcc
    25201 ccctagatga ggcaacataa gtatacggtc attttctaag aactcttctt ctggaaaatc
    25261 agtcaagtat ttagccagat gttgtttttc caatgcttgc ataacagcat atttatcaac
    25321 aatttcactt cttcctacat tgataagtac agcatctttt ttcatttttt ctatattcgc
    25381 ctgatcgatc aatccttttg tatcttctgt caacggcagc atgactacga tgtaatccga
    25441 ggtagaaaga actgtttcta aatctgcttg ccgaacataa tccaactggg catcgcggat
    25501 agaataaccc aagacatcca tccctaagct atagcaactt agcgctactt ttgtgcctat
    25561 cgctccaaga ccaagaagac cgatggtttt gccttgcaat tctcgtccca cataagcact
    25621 tcttttgttt tctgcctgtt ctaaaatatt aggacctgtt agtgtttgta ccatacgact
    25681 tgcttcgatt atcggacggg aagataagag caaacagcac aaaacaagct cttttactgc
    25741 attagcattt acacctggtg tattcatgac gatcgtcccg ttttcagaag ctttttcaac
    25801 gttgatcgtg ttcaccccaa caccagctct tgaaattgct agcaaatctg gtgtaagcca
    25861 ttcatctttc actttcgtac tacgaataac tattccttcg gctggcaaat catctattat
    25921 tttgaaatat tccccttgta acttttctat ctcttgttct ggaatagcat caatcaaatg
    25981 aacgtgatac ataaaacccc ctccttacta acaagtatct cgaaatttct gcaattagac
    26041 aactgtttac catttgactt cctagaagca aaaagagcag ttagagcaga gtaggctgag
    26101 tgataaaaat aaggctatga caagttactg ccataagcct tattttcgag agcaaaatat
    26161 tgagggtgag atatcttgat aaatggtgtt ccgttagctg acattgataa taagactaaa
    26221 tttcaaaaaa gttgaaaagc aaatttcgtc aatctcttct tatttcttaa aactaagtgg
    26281 ctttttcaca acctcttttt atctaataac cactgtttcc aactcaacta attttgccca
    26341 cgtttcaatt tgatctgttg ttagatttag cgagacaacg gtatgatgac cgccacctgc
    26401 ctgaatccat gaatggacac cttcatggaa attcggttta gttttccata acacacgtgc
    26461 aacaggtaaa ttaggtgctt cagtcgttgg ttcaaaggct tctacttcat tgatcagcag
    26521 tttataatgc gtaccgaaat ctgccatcga tacgactacc ccttctcctg ctttcccatc
    26581 aaatacaaga cgcgccggat cttctctatc tcccattgat aaaggagaaa caacgattct
    26641 tggtttcgtc gcagcaagcg tcggatctac ttccatcata tgggattcca agattgcttc
    26701 ttggccacta gctagctcgt aagtataatc ttccatgaat cctgtatctt tccctcgagc
    26761 catgattttc agcaaccgat caatcgctgc agttttccag tcgccttccc cagcaaaacc
    26821 gtatccttct gccatcaaac gttgtacagc tagtccaggt agttgtttca ttccatacag
    26881 gtcttcaaaa ttagtcgtaa aagcgttata gcctccttct tctaagaaac ggcgaatacc
    26941 gatttcctgc tgagcttgta cttttacatg ttcctcccat gtcttttctt cataatcacc
    27001 atagtcgaag gtatacaatt ccttgtactc ttcaaataat tgatcgactt cttcgtctga
    27061 tacgcgatcg attacttgta caagatctcc gataccaaaa taatcaacag tccaaccaaa
    27121 ttgaatctgt gcttccactt tgtctccttc tgtgacaccc acgtttctca tgttgtcacc
    27181 aaaacgagcc acttttattc caaaactttc attataggct actgcaacat ccatccaatc
    27241 agcaatctct ttttggatct caggtcgttt ccagtagcca acgacgattt tattttgttt
    27301 atttaatcga gcattgatga acccatattc gcggtcacca tgagcggatt gatttaaatt
    27361 catgaaatcc atatcgatcg ttttccaagg aatgctttcg ttatattgcg tagctaaatg
    27421 aagcaatggt ttttgtagaa gctttgtccc gcggatccac atttttgctg gtgagaaagt
    27481 atgcatccaa gtgatcacac ctgctacttc ttctcgataa ttgacctctt tcatgatttt
    27541 tgtgatcgta tcagcagtaa ctgctaattc ttgaaggcgg attggataag gcaattggcc
    27601 attttcattc agttcatcga ccatttcttg tgcatgtttc tttacttctc gcagtgcttc
    27661 ttccccatat aaatgctgtg atcctacaac aaaccaaaat tctttttctc caatattcaa
    27721 cataattagc cactcctttt atttgttttg tccgtaatac gcattttttc cgtgttttcg
    27781 taaataatgt ttatctaaaa tccgttgagg cagctcttct gaaaatgaat tgagctgtct
    27841 ggtaaataaa ttcatcttgc atacttcatc tagtacgact gcgttcatta ccgcactttg
    27901 cgcatctttc ccccaagtaa atggaccgtg tccgtgtaaa agtacccctg gaattgctaa
    27961 gggatcaatt ttgcgttctt tgaatgtttc tatgataact tttcctgttt catattcgta
    28021 cccgctgtcg atttcctgtt gtgtcaaaaa tcgagcacaa ggtaccgatc cataaaaagt
    28081 atctgcatgc gtcgtcccca tcgcaggaac atccaatcct gcttgtgccc agatcgtcgc
    28141 ccatgtcgag tgagtatgga cgattcctcc gatttgagga aatgaacgat ataaaacagc
    28201 atgagtaggc gtatccgaag agggattgag tttaccttca atgatatgat tgtccaagtc
    28261 cacaacgacc atgtcgtctg ctgtcatctc ttcataactg actccgctag gtttgataac
    28321 gaaatatcgt gtttcaggat caaaagcgct cacatttccc catgtatatt tgacgagtcc
    28381 gtgtttaggc aagtcaagat ttgcttggta tacttcttct ttcagttgtt ccagcatttt
    28441 ctatcactcc tttttcttgc aattgatgaa tcgctgtttt ttcaatgtcg atcccctcac
    28501 ggtaacgtct cataaatatc tgatagcctg ctatttcttc tgctgaaggt gtgatggtga
    28561 ttccttggtc ttgtccaaac acgtcttctg ccaagaagtt ttctaaagac ttcccatgtc
    28621 gatccatcat ataggctgct aataacgcga ttccccacgc tccaccttct ccggctgtgt
    28681 ccatcactgt aactggtgcc tccatagcag atgctagaat ccgctgtcca acttctggtg
    28741 ttttaaaaat ccccccatgc cccactaaac gatcaatttg gacattctca gatttcaata
    28801 tatccattcc gattctcatt gcaccaaaag cactggctaa atggatccgc atgaaattcg
    28861 ctaaatcaaa acggctacct ggtgtacgta caaataatgg ccttccttca tttactcctg
    28921 taatgttttc accggaaaaa taaccataag aaagtaatcc cccgcaatcc gtatcgcctt
    28981 ctagcgcttt gttgaataat accgtgaata atttatctct gcttatctcc atacccaaag
    29041 aattggtaaa ttcttcaaaa atactgaccc aagcattgat atccgaagag cagttattcg
    29101 tatgaaccat gcctactagt tctcctgaag gtgtagtgac catgtcgatt tccggatgga
    29161 cattttctag ttctttttct aatacgatca tagcgaaggc ggaagtacca gccgaaatgt
    29221 tccctgtccg tttggctaca ctgttcgtag cgaccatacc ggttcctgca tccccttctg
    29281 gcggacaaac cgggattcct ggtctcaaat ttcccgtagg gtcgatcaga taagcccctg
    29341 attcgcttaa cttgccagct tcttctcctg ctactagaac tttaggcaaa atgtgatcca
    29401 agtcaatcgc tacgtgcgct ttttcgatta actgaccaaa aatatttttc atttcttgat
    29461 ggtaggtttt cgttgaagga tcgataggaa acatgcctga tgcatctcct acgcctagta
    29521 ctttctcacc agtaagctgc caatggacat aacctgcaag cgttgtcaaa aaattaattt
    29581 ctttcacatg ttcttcttta ttcaaaatag attgatacaa atgagcgata ctccatctct
    29641 gcggaatatt gtattgaaat ttttccgtta atattcgttc tgcttcttca gtgattgtat
    29701 ttcgccaagt acgaaatggt actagtaact gttccttgct atcaaatgct agataaccgt
    29761 gcatcattgc actgaaccca atcgacccga ttgtcgtaag attctcgcca tagatttcga
    29821 atacttctgc tgccattttt cggtaactga cgcgcagtcc tttccaaata tcttctaatg
    29881 aataggtcca gataccattt tctagtttgt tttcccactc ataactacca gcggcaatag
    29941 gagtaaaaga tgaatcaatc aatacagctt tgatcctagt tgatccaaac tcaatcccta
    30001 aagatgtacg accatctatg acatctaaca tactttgcgc atttacttcc ttcataatga
    30061 tttaactccc tccttctcgt caaacctgat caaaccgtct ttttagtagc gcttacaatg
    30121 attattgtat acttttgtac gtacatgtca agcggttatt tacaagaata caataaaaaa
    30181 cacttgtttt gtacgtacat aagttataaa atgaaattgc ttacaaaatc tgatcaaccg
    30241 ccttctcgaa attctagaag gagagaatac ttatgaaaaa agaaaaaaaa gtttcctcat
    30301 cgtttattta tttttttggc gcatttggcg gcattctatt tggatatgat attggtgtta
    30361 tgaccggcgc cttacctttt ttacagcatg attggggttt ggcaggtaaa gcttctttga
    30421 taggatggat cacgtcttcg gtcatgttag gtgcgatcct tggcggctct ttatctggtc
    30481 ttctttctga taagcttgga cgacgaaaaa tgattcttct ttctgcactt atttttatgg
    30541 ctggttctgt cctttctgca tctgcccctc acaatggttc ttattttctg attgcagcaa
    30601 ggattcttct aggtttagct gttggcgctg catctgccct agttcctgct tatatgtctg
    30661 aaatggctcc tgctagacta aggggaagat tgtcagggat caatcaagtg atgattgctt
    30721 ctggcatgct cctttcctat gttgcagatt atttgctaaa agtgttgcct gaaacgatgg
    30781 cttggcgcgt gatgttaggg ctcgctgctg tccccgcgct gattttattt ttcggtgttt
    30841 tagctctacc agagtcaccg cgttttctta tgcaatctgg ccgcttggaa gaggcaaaaa
    30901 gagtattgaa ctatattcgg acaccaaaag aagctgaaca ggaattcgaa caaatccagc
    30961 tgaatgtcaa acaagaaaag acaaccggaa cttcttggca tactttgttt ttagaaaaat
    31021 atcgttcctt agtcattgcc ggtattggtg tagcagtatt ccagcaattt caaggagcta
    31081 atgctatttt ttactatatt ccgctgatcg tagaaaaagc gactgggcat gcagcgagcg
    31141 atgcattgat gtggccgatc atccaaggaa tcattttagt tgctggttct ttgctctttt
    31201 tggttattgc agacaagttc aatcggcgaa ctttattgaa aataggcggt agcgtgatgg
    31261 gactttcatt catcctacca gctgttttag gaactgtact ggatgctcat acaaattcgt
    31321 tgttgattct cttgttttta tgtatttatg tcgcatttta ttcttgcaca tgggctccac
    31381 tgacatgggt aatcataggc gaaatattcc cactagctgt ccgcgggcga gcatctggcc
    31441 tagcctcttc ttttaattgg attggttctt tcctagttgg cttgcttttt cctgtgatga
    31501 ctgcttctat gtctcaagaa atcgttttcg gcatttttgg gatcatctgc tttttagggg
    31561 ttttatttat tcaagagatc gtaccagaaa ctcgtgggaa atctttagag gaaatcgaac
    31621 aaagtgcatc taaaaaaact tatcctaaac gtatttctat ccatcattct taaagaggtt
    31681 gtgacagaag tggtcagctt caagaaataa gaaggaaccg cttagtttcc agtcacaagg
    31741 aacaatttca aaaaacagct tctcatgttt gatgaatatt gtgacttgtg accagttggt
    31801 aggagaacac cgtttagtgg agaggtggtg aaagaaacgt tcagccttga gcgatgacaa
    31861 aagctctcac aaacattgcg acgagtaacc gtaggagcaa taagaggcgg taaaaacccg
    31921 tctcttattt ttcgttttct ttcactgttt cccgttcaac tagtacaggt tcatagagaa
    31981 tatttggctg tgcctgtcct ttttcaatcg ttgtgacgat ccaccgtgcg gcatcttgtc
    32041 ccaacttttc ttttggatgc gtcaatgttg tcaatttttt attccctaaa tggcttaaag
    32101 aagaatcgtc tgttcctacg atcgataggt catctggaat atgatagcca ttttctgtca
    32161 acttatctag cattaaacta gctatttgat cgttatagca aatgatgcca tctaaagctt
    32221 gttcttcttt caaccgttgt actgtttttt cagcgattgt ttctcgtgtt tccgtggtga
    32281 aagtaatgat atcttccgtc aaaaaacgca cattatactg ttcacacgcg ctgataaaac
    32341 ctttcatcct gtattttcct tgaagatcgt cgatttttgt gatgaacaac agacgctgat
    32401 gtcctttttt tatcaaatat tctgctgcca taaaaccgac tttcacatca tctaaacaaa
    32461 tataagaggc atttaactct tcataagcag cattgatcat caccatcgga atgccttgtt
    32521 ctctcaaagc aacataaagt gctaagtttg ggttatactg attacttttc gtaggctcaa
    32581 cgatcaaacc atctacccca taagcaatca ttttcttcaa acactctttc tccaattcgt
    32641 gatcgttatt cgtactggct aaaagcaatg aatacccaga ttctcgtaat tcattttcga
    32701 ttcctcggat aatcgacgga aaaatataat cagagagata tgtggtgatg acaccgatcg
    32761 tttttgttct gttcaatggt gttttttctt tcttatagct gtcatctaca tatgtgcccg
    32821 accctttttc tttacgcaga tagccctcat ttaccaacac agcgattgct tgccgaatcg
    32881 tatgtctgct gacgtcatat atttcttgca gcttgaattc tgagggaatg gttgtattta
    32941 cttcgtattc ctcacgaagg atcttttgtt ttatctcttc tgcaatatct tgatattttg
    33001 ccatttgcct tgcccctttt tatttgtacg tacatatatc ataacgagtc tgcaaggttt
    33061 attcaagcaa aaatcaaggt ttcttgtgct cataaggcat acggaacagg ttcaccaaat
    33121 tctggaaatc cttgctcatt ccacgtaaag cattgcgcgt tcgcgtgccg attaggattg
    33181 tctaacggat cccctttttg gttcttctct ggtcgagcat gatagatcaa aatatcaaag
    33241 cacccgtctt ctgaaaccgt aaacgagttg tgaccaggac caaattgctt atttttttca
    33301 gaagaaacga atactggctc cgataattta tgccatgatt cttttaataa aagattcttg
    33361 tgctcatcag cccacagcag gcccatggca tagttttcat ctgttgcact accagaatac
    33421 gtaatgaata ttttcccatt gcgagtaagg actgctggac cttcatttac tgaaaaaccc
    33481 cgtttttccc attcatactc aggtatagaa aggaggatct gcttcccctt taacgtccaa
    33541 ggattatcca tttcggatag ataaagatta gaattcccag taatttcagg atcttgttgg
    33601 gcccacacat aatatagctt tccttgagaa gagaaagttg ttccgtctaa actgaaagtt
    33661 tcccttttcg tttttatttg tcccttttct atccaatctg tattcattgg atctatatgc
    33721 tgattttcaa gtacgaacat gcggtgatga tgagattcat cacgtatagt tttatgattg
    33781 cttgcagcaa agtaaatata ccattttcca ttgatataat ggacttcagg tgcccagatc
    33841 aattcactca tcggtccttc ttcatgagcc tgccatacaa tcaggcttgc agcatgttct
    33901 aattcattca gtgactttgc ccttcttaat tcaatcactt ggtagcctgg aaccgatcct
    33961 gtaaaataat aataaccatc tgtatgttta tatacccaag gatctgcccg ttgccgaacg
    34021 ataggattat tataaagtgt cttcatcata acaaatttcc tcacattttc tttattctaa
    34081 ttacattcca agagtatttt tctatcccta catttaattc gtcatgtttt tctattgaaa
    34141 aatagtttat ttcaggtttt attttttctg gttcttcacg tgtatttttg tccaacagat
    34201 cgctactggt taatgtgtag tgctctcctt gatctagcaa attgaaatca tgttcaatcg
    34261 ttatcttttg ttgttcattt gtgcgattga tcaagaaaat agtcagctga ttgccgttat
    34321 ctacgatcac actgtccaca taaggaacat tttttatctt ttcacaatca taaacagggc
    34381 aaatgatttg ttgtttcaat acttgacctt tagcatattt cgataagtgt tgcatcacat
    34441 aataagttgg attcctccat gcttgtcctt tagcatctgt cagtattaaa ggtattgtgt
    34501 ttactaacaa tgattgacaa ctaatcttta cgcgatctgc gtgtcgtaaa atagccagcc
    34561 ctaatgaacc aaataataat gtatcttcca tagtaaagta acaccaatcg atgggactac
    34621 cgacttcaaa atccctatgt cttttttccg gttgggcatg tacattccat tcatcaaacg
    34681 ctagatacat tgttttctca cttctcaaat atccttttac ataatcacaa gtagcaacga
    34741 tttcttcaat ttgacgatcg aatcgataag cttttgcaag gtatgtcgct gtatcatcac
    34801 gctcattttt aggaggttta gtcaagtctt cttcatcata cttatcaata tagttatgca
    34861 aagctaaata atctacattc tcataagctt cttctaatgc gatacgatcc cattcagggt
    34921 acgatggtaa acgtggcgta gagcttccaa ctaagattgt ttcaattgaa tcatccacta
    34981 acttcatgac ttttgaggct tcatttgcta acctaccata ttcataagca ctcttagccc
    35041 ccacttgcca tggaccgtct agctcattcc ctaaacacca taatttaacg ccaaaaggct
    35101 ttttttgccc attttttatt cttaaatcac tccagaaggt tccttttgga aaattacaat
    35161 attctactaa attttttgct tcatcaattc cttttgttcc taaattcacc gtgaatatcg
    35221 gtttcgcttg tacatgactg atccatttaa aaaattcgtg gagtccaaaa gaatttggtt
    35281 ctatacctcg ccatgctaaa tcaatttttt ttggtcgttg ttctttccta cctacagtat
    35341 ccacccaatc ataaccagat gtaaagtttc ctccaggata gcgaatgaca cctaattcta
    35401 attcctttac ataagagagg acatcttgtc taaacccgtc accatcagct gtttcatggc
    35461 ccggttcata aatccctgta tagacgacac ttcccatatg ctcaacaaat gagccaaaca
    35521 gccgagaact gatctctcct accgtaaaat cctctctgat ttttactttc acttcttcgt
    35581 ccaacagctt tccctacctt tcatttccta tagtcaagat aatagataca agtttccata
    35641 cgccaccagc aaagataaaa ataaaaggag ggaaaagaaa tatgagcagt acaaatccta
    35701 ataacaatac gcaacaaaga atatttctca aaaaatcaaa aaatactttc catgcacttt
    35761 gttcaaaaat ttgtaaaaat gtgatatctt tgttttcaga aatcacatat atccaggata
    35821 aaagaaacat ggaataaata agtaaaacta catatccact aattgaaagt acgattgtct
    35881 gttcttctaa taattgatta acaagaaaat aaataatgat cgttaacaga ctaaggaaca
    35941 agttcttacc aaaatgttgc tttattcctt gaaagaaaaa ataaatgata tttcctgatc
    36001 cctcaccttt ccattgcgcc attacataaa aaacagcaca tgtagcaggc acgatagtaa
    36061 taacaggcaa acacacgatt acccagagaa tacctaataa gatgtaatca gaaaccacaa
    36121 tcaactgtcc ataaaattta gtctcaacta ttttcatctt cctcactctt tcattccaga
    36181 catagctgtt ccttgaacaa aatatttctg tgcaaaaata taaaccacta gtaatggcag
    36241 cattgaaata accgttccag ccattacagg tccataaaaa gaaatgttct gccctgtaaa
    36301 taaagctaac cctgctggta aagttctcat ttttgaacta cttgtcaata tcaatggata
    36361 aagcaagtca ttccaactat tcatcagcgt gaaaatccct aatgataaaa gagtaggttt
    36421 gctcaatggc aacatgattt tcaaaaaaat attaaactca cttaatccat caatgcgtgc
    36481 tgcctcttcc aaagaatctg gtaaagtaat aaaaaaggaa cgcatcatga agataccaaa
    36541 tgcagttgtc atacgaggaa tgatcaaacc tgcataagta tcaagcatgc caagcaaatt
    36601 gacttcaata aatagaggaa tcatgaagac ttggaatggg atcatcattg tcagcaaaac
    36661 gaaatagaac aaaactgatt ttcctttgaa acgcatacga gcaaatgcat accccgcaag
    36721 agaatcaaag aatacagaag tgatcactgt ccctccagca aaaatcactg tatttttgat
    36781 gtaatctaat aaagggatcg ttgtccaaac cttaatatag ttatctaggg tatagctatc
    36841 tgcaaatatt ctaggcggaa atgagataat gtctttttcg tgtttaaaag aagaaataat
    36901 cagccacaaa aaaggaaacg caataaccaa caatataact agcatactaa tcgttcgaat
    36961 gattttttta ccactaactg cttttcgact attcacagta actgtaatcg agctttctgg
    37021 tgccatacag gtttccctcc tatttcattt gtttttcttt ccgtgacatg tacacattag
    37081 tggagatagc tatagttgca acaatgataa acaaaaccga agacagtgct gatgcatagc
    37141 ccaattcata aggtgctgta aatcctcgac tgtaaatata ttgaacagct gtttctgtct
    37201 taaattgtgg gccaccagcg gttgcaacat atacttggtc aaacacttgc atcgaaccaa
    37261 tcagattcgt taaaatacaa aaacctaagg agggaacgat ctgaggtagc gtaatgttaa
    37321 aaaactgctg tcgatttgtt gcaccatcca tttgagcaga ttcatacaaa gagggagaga
    37381 ttccctgaat agcagtcaag agaatcgtca tagtaacacc aaaattcttc caaacagtca
    37441 tgatggctac cgtcggcata gcaagggttg gatcgcgaaa aaattgaggg atcggcaagc
    37501 cgacttgtga aaacaagtaa ggaatcgccc ctacattcgg atcaagcaac atagaaaaaa
    37561 taatcgcaat tgacgtcaat gaacaaataa ctggaatgta aaatgtcgaa cgacaaaaac
    37621 gattgaatct ggtattttta gataatgcta ctgctgctaa taaaccaacg ataatttgtg
    37681 ttggcgtttc gagtacggtg aaatataaag aatgaatcaa cgaattaact gctcgttcat
    37741 cactaaatat acgtgtgaaa ttttcaaaac ttacaaaatt tcttgatgta aagaaaatat
    37801 tcaaatctgt aaaactaata gcaaatgttc ctaaaagcgg tatgacagta aataataata
    37861 aaactaaaaa agatggagtt aaaaacaaat acgcagcacg atcaggacga ttccaatatt
    37921 tggtgaatcc gttcatatag tccacctcat ctttcatttt gataaaatat acaatcttgg
    37981 tttaaacgaa tctgaataag atatccgaac aatcgctgtg atttttgcaa taacacctat
    38041 ctgcaaatac taggtcgatc attcagatat ctgtatcttg ccaacagtct atcctgtata
    38101 aagttctgta ctatttttgc agtaattgat tgatggcatc atctgcatcg ttcatgagtg
    38161 tctcaggatc ttctcctgct aataattttt caatcagtgg attgatgata tcgttattga
    38221 tcgcagagat ttgggtaaaa cctggtaaat atggctctgc atagtccatt tgtttcgata
    38281 attcagatac aataggatca ttttttactt cttcatcttt tgctactgaa tccaagtagg
    38341 caggaaaacc attttcgata ctccattttt taccaatttc agttgtattc caatatttca
    38401 aaaactcata gacagcttct tttttattat tatctgtact tgccggaatg gcatatccta
    38461 cgccatctaa aatagctgtt tttttgtctg aatcactccc ttgtggtaag gtagtcacat
    38521 tataatcaat ctcgtttgct ttcaatccgt tatttaacca tggcccattg atttcaatgg
    38581 ctagttgacc agcattcatt aaattgtcca tttcggcgcc agaaatcgat tctggaccag
    38641 tcttgtctac atggatcatt ttttggatcg cttttaacgt tttaaggttc tcaggagacg
    38701 caaattcaga cttagtataa tcatcgttca ctaatttacc gccatttgct aacatccagt
    38761 tatataacgg tgcagttcca tccttattga agacaaaacc tgatacattt ttactactat
    38821 cagttaattt tggtgccatt tctgtcaatt cttcccaagt agtaggtggt tgttcagtat
    38881 ctaatccagc ttgtttaaac aaatcattat tccaatacat atacatgcct tgtacctgca
    38941 tggggacaaa atactgttta ccttctactt cacctaattc aatagccgtt tctttaaaat
    39001 cagatttatc tactccatca tattcccaga aatcgtctaa tggttgtacc gctccgtttt
    39061 ctacatactg tgcaaaatct ggataattta atgcaataaa gtcaggtgct tttttagaag
    39121 aaatcgctgt aggaagtttt tcattcaaat tggcccaagt catgatatcc atttcaattt
    39181 tgatgttttt atcatttgtt ttattaaatt cattaacgat ttcttgcaat atttcaccat
    39241 cagatgcagt aaacccattc caaaaagtca aagtcacttc tttatcagaa tcagattctg
    39301 ctgaacttcc gccaccgcaa gctcctaaaa tacctgttgc agctaccaac aaagagccaa
    39361 ttaaccattt tttacctttc attttttgtt cctccttcat ctgataaacg acatctgacc
    39421 acattccaag atagcggttt caataaacaa ttgattttcc catcttcagc ttttacattt
    39481 gttgaatgac ttggttttac ttgttcactt ttaggaagat tagttgcttt tgtatcaaat
    39541 ccactcattt cagaaaaatc aatcactccc tctaatgata attcagacaa gtgaacagtt
    39601 agattcattt cttcttctcc acggttaact gcaaatatga caagttcttt gtcatttatt
    39661 acggctatac tttctaaata cggcacttct tcaaaatctt ccgtcgcata gtttgctgaa
    39721 cgaatttgtg ggcttaacac ctctccttgt ccatacacag cagcttgcat aaatggataa
    39781 tagatcgtct gtctccaagc ttctccgttt gtttctgtcc tgattggtgc aataacgttt
    39841 accaattgag caagacaagc aatctttact ctatctgcat gtttcaataa agtgattaat
    39901 agacagccta atagtaatgc atcttcaaaa ttatagttat cttctagtaa aggaggagca
    39961 atctgccaag gcgctgcttg ctcatcttgt tccgctgaat gataccaaat attccattca
    40021 tcaaaggaca ggttgatctg cttcttacta tgtttcttcg ccttgactgt atcgcacatc
    40081 gctgtcactt cagaaatgaa ccgatccatg tctaaggaac gtgcaagata atgagaaaga
    40141 tcattttttt ggttgccata atattgatgc agagacaaat aatctatttg atcgtacgct
    40201 tgatcaagaa ctgttaactc ccaatcacca aatgtaggca tttgccgatt cgaacttccg
    40261 cataaaacca cctcaattga atcatctacg agtttcatcg ccttagccgt ttcctctgct
    40321 aaccgtccat attcttccgc tgttttatgt ccaatctgcc aaggaccgtc catttcattc
    40381 cctaaacacc atgtcttaat tccaaaaggg ctctcttgac cattcgaacg ccttaaatca
    40441 ctccaataag ttcctttagg aaaattacaa tattcgacta aattacgagc tgcatctact
    40501 ccacgagtac ccagattgac agccatattg atttcggccc ccacatgctg acaccagtta
    40561 taaaattcat gaatcccaac ttcattcgtt tcaattgtcc gccaagctaa atccaacctt
    40621 ctaggtctat ttgctatggg cccaatactg tcttcccaac gaaatcctga cacaaaattt
    40681 ccaccaggat atctaatcat gggaatgttc aattctttta ccaagtgaat gacgtcttgg
    40741 cgaaatccac ttttattagc tgatggatga tctggctgat aaatcccttc atatactgct
    40801 cgtcccatat gttcaataaa tgaactgtac aaccgcggat caactttgcc tacacgcttg
    40861 acactactaa tcaacattgt ggcttccata actgacctcc tttaatttat acatgaataa
    40921 tacacctcgt tgaaagcgtt gtcaataata taaatgttaa acttacttat tttttatcga
    40981 ttttaattaa ccaaaatatt aataatgatt aaaacaaata atttaatata acttttatta
    41041 acaaatatgt taatatataa gaaaaacctt ttaagaggtg atatatatgc aactacaaat
    41101 aaacgaagaa tccttaccgg tatatgaagc attagctagt aaaacaagga tcaagatcat
    41161 ccagctactt tccaaaaaaa agatgaatgt caaagatttg gcaaaagaac ttggagtgag
    41221 cagtgctatc acgacgatgc acgtgaaaaa gctagaggaa gcaaacatca tcaaaacaga
    41281 aaaagtagga caacaaaaaa tttcgagcct tcgtgtcgat aaaatcgata ttagttttcc
    41341 agaaaaaata ttcaatgctt ttgatacgaa agaaacatcg atacctattg gtcactatac
    41401 aaattatgca attgaaccaa catgcggttt agctacgatc catgatttta tcgggaaagt
    41461 ggatgaacca cgctatttta tggatcctcg aaggatggat gcccgcattc tctggtttac
    41521 cagcggattt gtcgagtatc aggcacctaa ttttttaaat acagaggata cattggagat
    41581 gttggaagtt tctgtggaaa tttcttctga atttcctttt tcaaatgaca attggccttc
    41641 agatatcact ttcagcctaa atggtgtgga gctaggaaca tggacgagcc ctggagattt
    41701 tgctgatatc cgcggtaaat atacgcctga ttggtatcca gataatctta atcaatacgg
    41761 cttgctgaag acgatccgaa ttaccaaaca cttgacgaat atgaatggag aacccttgtc
    41821 aaacatcacg atcaacgaca tccctaaaga gcaagacact tggcatttgc gaatcgaagt
    41881 gaaagacgat gccaaacatg tggggggctg tactttattt ggtaaaggat tcggtaatta
    41941 cgatcaggat atcaaattaa aagtttacta cagctgatat ttttcttttc tattttataa
    42001 aacaaaaaca gccaatccct atgaagggac ggctgttttt gctttattgt tatttccaga
    42061 aatcatccaa aacggaaatt ggcaaatgtc gtttatgttg tgtcttgttc caccatgctt
    42121 cgattttttc ttgtgcttga gcgggaactt cttttccttc tagataatca tcaatttcat
    42181 tatatgtgac acctagtgcg acttcatctg caatcattgg tttcccatct tcaagatctg
    42241 cagtcggaat ttttgtgtat aatttttccg gtgcattcaa tgcttgaagc aattgtttcc
    42301 cttgtcgttt gtccaaacgg aataatggca aaatgtcggc tccaccatca ccaaatttcg
    42361 tgaagaatcc agtaatgttt tctgcagcat gatctgtccc taatacggct cctgctcttt
    42421 ctccagctac ggcatattgc gtaatcatac gttgacgtgc tttgatattt cctttgttaa
    42481 aatcgctgat tcccacacct gcttcgctta atacttgtac ttgtgcatca acagctggtt
    42541 tgatatttac tcgcagacta acatcgggct ggataaagtc aagagcagct tttgcatctt
    42601 cttcatctgc ttgttcacca taaggaagac gaacagcgat aaattgatag ttttcatctt
    42661 ttgtttcttc acgcatttct tccatcgtta gttgcgccaa acggcctgct aaagtagaat
    42721 cttggccgcc gctgatacct agtacaaaag ttttaagaaa cggatatttt ttcaaatatg
    42781 ctttcataaa gtcaatactt ttacgtattt cttcttttgg atcaattgtt ggtttgacac
    42841 ctaattcatt gataatttct gtctgcaggc tcatttattc tcctcctatg cttctgtatg
    42901 aagttctgct acgctttttt tcattcgttc catgatagcc attttatggt tccagcattc
    42961 agtagacaga tcgactgggt atttttgcgg attcaagtct cgtttatact cttcccatag
    43021 tgaatctaga ttttcttttg tatattcttt gatttcgttt agtgcaggta attcgtaaac
    43081 tctttttcct tcgataaaga tatcttgtaa aactgggcga gctgtaaagt ctcgaacagt
    43141 tttattgata aacgtatgca ctggatggaa catgaagata gcttcttctt ctcgagggtc
    43201 ttcatcccat aatgtcacgt agtctccttc agatttccca tcaaaatttc gagtgatgcg
    43261 ccacacttgt tttttccctg gagtagttac tttttctgca ttgcttgata acttgatcgt
    43321 atccatcatt tttccttcat catcttcgat cgatactagc ttgaatactg caccaagggc
    43381 tggctgatca taggctgtga tcaattttgt cccaacaccc catacatcaa ttttagcttt
    43441 ttgcatcttt aaattcaaaa tcgttgcttc atccaagtca ttcgaagcat agatttttgc
    43501 ttccgtaaat ccggctgcat ctaattgttc ccgaacacgc ttagatatat aggccatatc
    43561 tccactgtcg atgcgtacgc cttggaagtt gattttgtca cctaattctt tcgccacgcg
    43621 aatcgcactt ggcacacctg atttcaacgt atcataagtg tcgactaaga acacgcagtc
    43681 tttatgggtt ttagcgtagg ccataaatgc ttcataatca tttccataag attggactaa
    43741 cgaatgagca tgggtcccgc tagctggtat cccaaatatc ttcccagcac gtacattgct
    43801 tgttgcatct gctccgccga tatatgccgc tctagttccc cagactgctg catccagttc
    43861 ttgtgcccgt ctagttccaa attccaatag tggatcttct ccgatgacag atttgatacg
    43921 agctgctttt gtcgcgatca acgtttggaa attgaccata ttcaacagtg ctgtttctac
    43981 taattggcag tgcgctaaag gcccttccac ttggatcaat ggttcatttg caaaaactag
    44041 ttctccttca cgagcagaac gaactgttgc tttaaattca aaattttgta aatactccaa
    44101 aaaaccttca ggatagactt ctaatccacg caagtaagca atatcggaat cattgaacgt
    44161 cagattctct aaataattga ctaaccgttc caatcctgca aaaaccgcgt atccgtggtt
    44221 aaaaggcatt tctcgaaagt aacattcaaa aacagcatgt aaatcagctc gacctaattc
    44281 ccagtatgtc tgcatcatat tgatttgata tagatccgta tgcagcgtca aactgtcgtc
    44341 tgggtaagta ttattcatct tgtattactc ctcttggcaa gttttcattt cctacccatt
    44401 ataacaaaaa aaattaatag gaaaagaaat cttccatttt ttctgaaaaa gcacatgttt
    44461 tacggatatt tcttgctaaa aatgaactcc atctgctaaa actatccttt cttattggta
    44521 tttaacctaa acgagaaaga atacttattt tattcaatgt atccgagaca cgaagaaatt
    44581 agcgttttct atttttctta cttgataaaa taagtagatt ttcctactac aatcgtagcc
    44641 gttaagatac cgatgattga acaaatataa ccaaatgtct ctacaacata atcaatggca
    44701 aaagtttttg aagcaataat acgtctttcc ttttttgctt cttatcccca aagaaacgga
    44761 tataacttat tatgtattga atttttttgg aaaggcgaat tatgacaaga tataccttgc
    44821 gttgaaaaaa ctcgcggaaa aaatatcaca cgtttttaaa aagatacaaa gtaagaaagc
    44881 agctgtcaat agaatggaaa caaaaatggc gagacatcaa tcagtctcgc cttgtttttt
    44941 attgtataca ttagatatgg agcaggttac cataaagatg attattagtc gttattaaat
    45001 agaatccttc gtattttttt gttctatcac tacaatatct ttatttggac gtttgaattg
    45061 tattccggca cgttctagct cctgtttcac tcctacatgg actttgacat cttctcgtgt
    45121 catttttaaa acgtgatgaa tcgcatagtc gatttcttgc cttgtgattt ttttcttcgt
    45181 attgatcatc acgacttgct ttttatcata tgtgtctacc aatagcagtt gtgttcgatc
    45241 taacgaaaat aatttgtaat aattaatagc cgtattccca aaaaagaatt ccactacttt
    45301 aggataaaat gtcttaatat ttaaattact attcacaggt gtttcgatta gcttcatctc
    45361 attcctcttt tctatatagt cgatttagat ccaatcttaa gttatgttac catctttcct
    45421 agcattaagg aatcggttac aattgaacct ttaaagacaa aaggatttac cttttatcac
    45481 ttaatagtat aacataattt catgagaaaa aaatacaatt ttttcttgac catctcgtta
    45541 cgtaaaggat tatgcttaca tttgtcagga ggaaaaataa tggagtacac aataaagaaa
    45601 atgtcagaga tatcaggagt cagtccgaga accttgcgct tttatgatga gattggactg
    45661 ctaaaaccag ctaggatcaa ttcttcaggt tatcggattt atggcaaaaa agaagtcgat
    45721 cgtctgcaac acatcttatt ttatcgaaca atggcattca agttggatga tatccaagag
    45781 gtcttagaca atccatcttt tgaccaccag aaagctttaa tcaagcatca gcaaatgttg
    45841 ctggaaaaac gggcacaaat cgataccctc ttgacaactg tccaacagac acttgatatg
    45901 tacgaaggag gaagaaaaat gagtgacaaa gaaaaatttg aagggtttaa acaacaaaag
    45961 ttgaaagaaa atgaagaatc ctatggacaa gagattcgac aaaaatacgg aaaaaaaaag
    46021 gttacagcat ccaatgaaaa atggatgaat atggataaag aaacgtatga agaaatgcaa
    46081 actgttgaaa agcagatgtt atttgatctc actctttact taaaagaacc agaagatgaa
    46141 agcttagcag accgtatctt ccaagctcat aagaagtggt tatcttacag ctggcctgaa
    46201 tatcagccaa aagcgcataa aggactagga atgatgtacg aagccgatga acggttcact
    46261 gcatattatg acgagcgaag tggtcatggt gcagcaaaag ctttaaatga aattatccaa
    46321 aaaaaggcgt aatcttttac atgatccaac aaaaaagtac acgtaaaatg acctaataac
    46381 aaaacagcag acacccaagt tagattttta tgctaacttt tggtgtctgc tccattcttt
    46441 cacaagtttt ttcatcctac tttaaatata ttcatcgttg aataaacgta taaggtattt
    46501 tcactttatc ctttttttct gtaagaaaca attgaaatgc catttctccg caagcttcca
    46561 gatggtggtc gatggttggg aaaccaagaa gctcgctgat cagcaaatta tcgcgtccaa
    46621 tcaccactgg tgtcttatct tgcagatagc tttgcaagat gcctgctgca accatatccg
    46681 aatttgtaaa gacaccatca actccaagct ctttaaaaaa gaagcctgct tgttttcctt
    46741 tctctgcatc tatacaatcc caaaaaacaa gtgattcatc aaaagatgga aaaagttgat
    46801 gacatagctg caaagtgatt tttgagttat aacttagcct gccacttcgc cccaacgtga
    46861 tgcccaattg ctttatacct tgctgcttca aataagtaag accttctgta attgattgtt
    46921 ctcgatcaat aaagacacag cttgcctgtt ttcccttgat ttcttcacaa aaaactagag
    46981 gtccatattt ttgatatgcc aataaatcat ctagtgtatt ggcacgagac gtaatgatca
    47041 atccatcgaa agctttagct gcaaattctt caagatacct tcgctctttt tctttctgat
    47101 agcccgtcgg taaaagtgtc actttgtaat cataatcaaa cgctgcttca atgatcccac
    47161 tgatcaactg atcaaaatac ggatgattgg tataaggcag aatcactcca atgcttttcg
    47221 tttttccata acttaactgt ctggcatttg cattagggat ataatcgact tctttcatga
    47281 ccgcaagtat ttctttcctt ttttcctcat ctacatatgg atgtccattg attacacgtg
    47341 aaatagtaga aactgaataa cctgtgagct ttgcgatatc tcgaatgttt gccactgctg
    47401 cttcgccacc tatacaaaaa gattttcttg taaatagtct accacagctg gaccgcattc
    47461 aaaaaactga tacatttttc cttcatgcga aaaactgatc cgctgcccgc cccaatatat
    47521 atcttttgtc aaattttcga tttcattcat tggtatttct tttactatat tcctattcaa
    47581 cccttgaaaa ctgtacaacg ttaatacgga tcccttgaac tcgacaaacg cataaaaata
    47641 attgttcgtt gtgccatctg ttatctctac ataagtattc attgcaagcc ctccttttga
    47701 tactttgagt atagtgtatg aaacgcgtct catagcaagg agtttgtttt gccaaagaaa
    47761 agttttattc ctctttcatt attaccaaag tctcttattt taaattgaat acattataat
    47821 gatactgtaa atatatcttt attattagtg aatggatatt tacttttaat agataaaata
    47881 ttataactat taaaaataac tattttcatt ataattacac acaaaaaaga gcgtgagaca
    47941 aaagtaaaga acacttttgt ctcacgctct aaacacgtat aaacagcgga agcagaagca
    48001 actccttcgg aaataagctg aaattcacaa aaatttgaaa agcaatttcc gtgaatttct
    48061 tcttatttct tggagttaaa cgcttctgtt ccagcctctt tttatctgtc tttcactaat
    48121 gatcagttgt agataagcaa gttgcttttt cttacatcat accgcccatc attgatggat
    48181 ccattgcagg agctgctggc gctgctggtt ctggtttatc tgcaacgact gcttctgtcg
    48241 ttaataacaa ggcagaaacg gaagcggcat tttgcaaggc agaacgtgtt actttagttg
    48301 ggtctacgat acctgcttca accatgttta cccattctcc agtagcagca ttgaaaccag
    48361 tgcctagttc cacgttcttc aatttatcaa caatcactga tccttcataa ccagcgtttt
    48421 cagcgatttg acgaattggt tcttctaaag cacgaacaac gatcttgata cctgttgcca
    48481 cgtctccttc tgcttctaca gcagaaactt tgctgataac gttcactaga gctgtaccac
    48541 cacctgagac cattccttct tctactgcgg cacgtgtagc attcaatgca tcttcaatgc
    48601 gtaattttaa ttctttcaat tctgtttcag tagctgcacc aactttaaca acagctacac
    48661 cacctgctaa tttagctaag cgttcttgta atttttcgcg gtcaaaatca gaagttgttt
    48721 cagcaatttg gtttttgatc aattgcacac gagcttcgat tgcttctttt tctccagaac
    48781 cttctacgat cgttgtattg tctttatcta caacaacttt agaagcattt cctaagtttt
    48841 cgattgttgc atctttcaat tctaaaccta gatcgtcagt aattactgta ccacctgtca
    48901 aaatagcgat atcttcaagc attgctttac gacgatcgcc gaaaccagga gcttttacag
    48961 ctactacgtt gaatgttcca cggattttgt tcaatacaag agttggcaat gcttcaccat
    49021 ctacatcgtc agcaataatc aacaatggac gtgattgttg caagatttgt tctaacaatg
    49081 gcagaatatc ttggatgtta gagatttttt tgtctgtgat caagatatat gggttttcta
    49141 gaacagcttc cattttgtcg ttatctgtta ccatatattg tgataagtaa ccacgatcaa
    49201 actgcatacc ttctacaaca tctagttctg tttcgatccc ttttgattct tcgattgtga
    49261 tgacaccgtc gtttccaact ttttccattg catcagcaat caaatgtcca actttatcag
    49321 aacccgaaga aacagctgct acttgtgcaa tggcttcttt tgaatcaaca acagttgaga
    49381 tattatgcaa ttcttcgact gctgcttttg ttgccaattc gatcccgcga cgaatgccta
    49441 gaggattagc acctgcagtt acgtttttta aaccttcgcg aacgattgct tgtgtcagaa
    49501 cagttgctgt cgttgtaccg tctcctgcaa tatcatttgt ttttgatgct acttctgata
    49561 caagttttgc acccatgttt tcaaaatgat cttctaattc gatttctttt gcgatcgtca
    49621 caccatcatt tgtgatcaat ggagagccgt aagatttttc tagtacgaca tttcggcctt
    49681 taggtcctaa tgttactttc actgtatctg ctaatttatc taccccgcgt aacatagctg
    49741 cgcgtgcgtc ttctgcaaat ttcaattctt ttgccatgat tgattcacct cattgattat
    49801 ttttgattat ttttttatct tccaaattta ctcaacaatc gccatgatat cttttcctgc
    49861 gacgatcaga tattcttttc cttcgtattt tacttctgta ccggcgtatt tttcgaacat
    49921 aacttggtcg cctgctttta cagcggcagg aactttttca ccgttttcta acaaacggcc
    49981 ttcaccgact gcaacaactg tcccagtttg gggtttctct ttagcagcag aagctaaaac
    50041 gattcctccg acagtttttt cttcttcttt tgcgacttcg ataatgacgc gatcacctaa
    50101 tggttttaac acgataaatc cctccgataa ctcttttttt aatctgttag cactcttaac
    50161 cttcgagtgc taacctacat ttttaataat actcattctc cctctttttt gcaagctttt
    50221 aaatcaaaga aaagaaattt tattctgaca gctgcttatt gaaattatga tataattgaa
    50281 atcaatcgat ataagcttgc cttgcatgct tgttatccct taaaaggagg aattacatgt
    50341 ctttaaaaaa atatagcttc ctcagcattt tttgctatgg tctaattttt atctcaccct
    50401 tgatcctagc ttcgatagga cttgtaaaat ctacgtctga attgatcaca gcaactgcag
    50461 tcatgtatat actaggcgcc gttgtcctag ctatttttta ttttaagcaa cgtgaaccct
    50521 tagcgatcga atcagcggta aaaaaagctt ctgctccaaa gattgttatt tatggattgg
    50581 ttggaatctt tgtcgcattg attctgcaaa gcatcgctgt catgttagaa tctgtattgt
    50641 ttggcaaagc aaccccttcc gagaatacac aaaatatcat ccagatgatc atggaagcac
    50701 ccgcttttat tttggcgaca actgttgctg gacccatcat ggaagagttc gtattccgcc
    50761 gcagtatact ggggatcatc agtcgatatt caaatttttg ggtcggagca gtgatcagct
    50821 ctttgctgtt tgcgtttgct cataatgatg gtcatctgct aatctacttt ttcctaggtt
    50881 tcttcttcag cctagagtac aaagcaactg gtcggatctg gacatcgatg atcacacacg
    50941 taggaatgaa cacattagtt gttcttgttc aattagctgt ccaaaaaggg ctagtataaa
    51001 catgactata aatactcctg tcatcctaga ttggtgattt tcccaatcta aagcgacagg
    51061 agtatttttg ttttttagtc gttcatcttt tctgctgctc catcattttt tgtctgtctt
    51121 tcctttttgc agtttgcaga atctattttt ttcaaaaatg ggaaataaat gaaaaaacta
    51181 attgacaaca cgataccttg taaaatcgct gcaggcactc cgcctactaa gaaacccgag
    51241 aggatcggcg gtgttgtcca agggacttgg actgctgtaa ataaaggaac gaatccaaaa
    51301 tataaagcgg aataggtaat caatcctgaa acgaccggtg cgaaaataaa aggaacagcc
    51361 atcatcgggt tcatcacgat cggtgtggaa aaaataattg attcattgat gttgaaaaag
    51421 gctggtaaaa gagcaagatt tcctaattct ctgtatttct tcgatttcgc aaaaaccgtc
    51481 ataaaaaata cagcccctaa cgtcaatcca gcacccgtaa ccgtcatgaa ctgatcgaga
    51541 aattgctgag taacgatatg tccgccattt gccaccgtca actcttgtcc tgaggctaag
    51601 atttctgcat ttgctaatga attcgcttgc aagacgggac taaccaaccc attgaccatc
    51661 gcagatccat gaagaccaaa taaccataaa aacggaacaa aaaaacctaa taaaagcaca
    51721 ccaaacaacg tgtctatgaa cgattgaatg ggaagctgga catatgtcca tactccttga
    51781 acaacagtca ttctgaacaa atgttgaaaa accgctgaac cgataagcgt aaaagtgact
    51841 ataacaatga ttggaatcaa tgaagtaaaa gactgccaaa cattttcagg tatcttctct
    51901 ctttgcttcc accctttcgc aagtcttact aaacgtgcat agccccatcc gatgatgagc
    51961 ccagttgtaa ctgctcctag tatcccttca gcacctgtcc agacagtatc tatctgagta
    52021 tttagtggct gaataagcag tagataagca gacaaggctg ttatgccgac tggtaagcga
    52081 tcgatgtctt cttcttttgc ataagcgtat gcaatggcaa aaacgatcca aaaggctatc
    52141 acatcaaatg tagcctggta tacttgtgta aaatacatag aaaatcctgt tttgtctatc
    52201 cactgagcga tgtaatccac aggaaaatga gcaaataaga aaaacagcga gccaacaata
    52261 attattggga tcgtgtaagt cattccatta cgcaaagcag ttaagggtct aagattcaca
    52321 acagatgtta tcattggtaa aaaggcacga tctaaccact ctttcatttc ttatctcctt
    52381 caacttctat ttttatcaat cagttttctt aatttaacta tacccaatga tcacgaacca
    52441 tgaaaggaat gcgcactgag cttttcaaaa atcggattct ttgacgaata ttcagcaact
    52501 gcttacaatg gacaggaata aaaaaggagg ttttttatga aaattgcact cattggagct
    52561 actggccatg caggttcatt tattttagac gaagcattgt ccagagattt agacgtgacg
    52621 gcaatcgtgc gtcatccaga aaaattgcca tcagatgttc cgtttataca aaaggattta
    52681 tttgagttga caactactga tcttgcatct tttgatgttg tcgttgatgc tttcaacgct
    52741 cccaaaggaa aagaagaaat gcatcaaact tctttgtccc atttgattga gatgcttgaa
    52801 ggaaccgata cacgcttaat tgtcgttggg ggtgcctctt cgttattctt agatacagaa
    52861 aagacgactc gtatgattga ccaagtacct aaaaatgcac ccttctatcc aacagctttc
    52921 aatatgtacg aaggattgtt aaaattgaaa caatctaaaa atctacattg gacttatatc
    52981 agtccagctt ctttatttga tccacatggc aaacgtacag gtacttattt gttaagtgat
    53041 gattatttac aaagaaacgc tgctagcgac agtacgatca gcatggcaga ttacgcgatt
    53101 gcattagtag atgagatact ggatgagaaa catgaaaagc agcacatcag tgtagtcagt
    53161 cgataatacg gttatttttt taatatgcta aaaaaataaa aatagaagat aaaaccaacc
    53221 ctgattgatt ttatcttctg ctttttatat ttttattgat tgaactgttt aaatcgaaaa
    53281 tagctcagtt gcttcatctg gatctgtatc atagatttca aaatcatgtc caacgccaaa
    53341 atctttttct ttcaaaacat tttccattgt taaatgagca agttctttca tattgttttc
    53401 cttgctaaga tggccaagat agattcgttt ggtccgatta ccgatcacat cagtcatgac
    53461 aagtgcaccg tcttcgttgg aaagatgtcc tctatctcct aaaatacgct gttttaaatt
    53521 ccacggataa ggtcccatgc gtaacatctc aagatcatga ttactttcca tcaaataacc
    53581 atcggcattt tcaatgacac ctcgcatata atcactgcaa tatccggtgt ccgtcaacat
    53641 gacgaatgac cgattttctt tatggaaacg ataaaattgc ggagccgctg cgtcatggga
    53701 gacgccgaaa ctctcaatat cgatatcgcc gaatgttctg actttcccca tctcaaatag
    53761 atgtttttgt tccactggta catttccaat cagtggttcc atcgcttccc aagtctgctg
    53821 attggcaaaa acatccaaat gatacttacg agccaagaca cccacgccat gtatatgatc
    53881 tcgatgttca tgcgtcacaa gtattccatc taattggtcc ggtgtccgat caatttgagc
    53941 catcaaggaa gtgatttttt taccgctaag tcctgcatcg accaaaagcc gttttttttc
    54001 actttcgata taaagtgaat tacccgaact tccacttgct aggatactga ttttaaaagc
    54061 tggtttttct tcagccattt gactcctact tcccttctta ctttgtaaac ttttttcatt
    54121 atactcgctt ttttattgat cttccacttt tggtacagta ttgtttgtca cgatcgtatt
    54181 gctgaaagcg ttgacgctct caatttggac cactttatcg cttgtttcga tcgctacatg
    54241 ccaaacagga acatacacat ttttttcccg gactttcaga atacaggaat aagctagctg
    54301 acgccataaa atttttgatc ctcttggtat tttgttattg atgtacaatg tattgattgc
    54361 ttcctcttcc gtatacagtt ccgtcttttc gcgcagctgc tcgatatcag ataaatgcgt
    54421 ctgcatgtat ttcgtgatgc tataatcatt atcgtttttt tctaaagaaa gaacgatttt
    54481 tgctgtatca tcattgaatg ggatgccttc atactcttgg cttgcttgga tttctagtgg
    54541 ttcttcttct aaatgagaaa agtttttcgt gtacacataa tcattaccga ataaaatatt
    54601 gtctttgtta gataaaaaat cttccaagga actactaatt ttatctggat cgatcacgtt
    54661 cttttcagca tctgaactgg ataaagattt cgtcaaaaca tcaccgtcta aaaacacacc
    54721 acttgccagc aagccgcttt taccagcctt gcgataattc gctaatgaag tgctaagatt
    54781 ggtctgctcc gcgctaagat aatagccttg cttgttctca gacgaaatct tccccgtgta
    54841 agtgatatcg tcgctagcca gacgttgttg gattgattca gtctgattag agcgaatgac
    54901 attgttttct tggtataccg cgtcatgata actgctgaat aaaaacacat tcaaccctaa
    54961 aaaagcgacg aaaaatatcc attcgatttt tttgaaatcc aattaattcg cctccaatcc
    55021 aggtaatttt tccagaaggt cgttagctga ataccagtta tctttatatt tcacatacca
    55081 ttcaggtaag agatcaacga cacgattcgt ttctttgaca ttttgccatg tatagccaac
    55141 gatcactgaa ccaatcttgt tgctatctgc tcccttagct tttaactgct ggatgatttc
    55201 attcgtactt gcaagttcta cttcttcgtc tgaaggtatc ggcacttgaa tcgtattcaa
    55261 actggtttgg atattcactt gcacagaacc attcgtttca ccgccaactt ccacaccaat
    55321 ctgtcctctg ctgtctgtcc caaaaaccgg gaagccttcc acgaatatcc gataatcgat
    55381 cgtattgtca ctgcggtcaa aatatctgag gttccctaaa gaactgccta atttgctaat
    55441 ataggggaaa ctttcagaaa atacactagc attttctttt ttctctggca gctctccacg
    55501 aaaatcaact acttgcgttt ccgaatggat agacattgtt tcattcccac cgtaaaaaat
    55561 caattcattg ctttcatcat tatttttcac gctatctggt ttctggaaaa aagcatttct
    55621 aaaaagtgta tagggttgct gtgacaggat ataactatac ttcttcagtt tgatcgaatg
    55681 tttcgtagca tattgcgtgt cgatcgtcga tggttctgtt gtcatttctg tccattttgt
    55741 gtcttttgct gttaattctt tattgatatc tgtccaattc aacgaaatat ttgcttcata
    55801 tacgagatgg ttcgaataat tcaaaaatcg gattttttta tttccttgat cgaattgtat
    55861 tctagtaaaa tacatatttt cgctttcttt tgccaagttc gacacatcta aatctaaatc
    55921 aaatgcttcc ttgtattctc ttagcaaaaa aggcgcatta taggccaact caaaaccaga
    55981 gttcagattt ttcacctgtt caaactgtga tttgtctcct gaaactactt cagaaagctt
    56041 tccaaatctt gctttatcga tgatcaattg caatcggtac acgagatttt cactgttggt
    56101 ttcttggatc attcccgaat ggtgccatgt aacatgaagc ggaagaaaga cttcggaagt
    56161 ttttcgattg ctttgagact cgatcatttg agtgtttgtc tcatctacgt tgattgaact
    56221 ttttcctgct gggctcagcc agattgcata agagaagtat atgcttaacg caaccatcaa
    56281 aatgagtcct atacgaacga ctttttcaga gattctcatt cccaccattc ctcctcataa
    56341 ggttcatatg gcagcgagat ataaaaagtc gacccttttc cttccttgct ctctgcccaa
    56401 atagcgcctc gatgcgcctt aatcacttct ctagaaatag ctaatccaag tcctgtccca
    56461 ccttgtgctc ttgcacgtgc tttatctact cgatagaacc gctcgaacac tcggttcaga
    56521 tcttttttag gaatccccaa tccttgatcg gtgatactta aaatcacgtt attatgcgtt
    56581 tctaataagc gacaagtaat cgttccccca tctggtgaat acttgatcgc gttattcatg
    56641 atattatcga ctacttggat gattttatct gtatctactt cgacccaaag atctctttgc
    56701 gtaaattcac gacggatctt ataatttttt tcctgatttc ccaccatcat atcaaaccgg
    56761 tcaagaacaa aattcactaa ctcgttgaaa ttgacatatt ctaattgaag ctgggtatta
    56821 cctgtatcca tgcgagatag atttaagagg tcattgatca ttcgaatcat tcggtctgtt
    56881 tcatctagcg ttaccttcaa aaaatttggt gctacttcct cgtctttcca cgcaccttcg
    56941 ctcaatgctt cgatataact acgcatactt gtcaacggtg tacgaagttc atgggaaaca
    57001 ttagagacga attcacgacg ttcccgttct gttttttctt gttctgtcac atcatgcaag
    57061 acagctacta atccgctgat aaaacctgat tcgcgacgga tcatagaaaa gtctactcgc
    57121 aagatcatct gatcactttc caatgtactg gttgaacggt caagtaccat ttcgtttgga
    57181 tcttctaata atttccgcaa cgtatattct tcttcgatat ctaaaagtgt caaaatcgat
    57241 tgtccgattg cttcctcgtt tttgatatct agcaaggaaa cagccatatc attgatcgta
    57301 atgactttac ccctgcgatc tgtcgcaatg acgccgtctg tcatatggga tagcacgcta
    57361 tccaaacgat tgcgttcagc ttccattgta tcttgagctt cttcaatacg ttcagctaac
    57421 tgattgaagg tatcagccag ctgtccaagt tcatcttgtc catgcacttt gactttccga
    57481 ctgtagtctc ctctagcgat tcgaatggct tgttctctca tttcaccgat cggctgtgta
    57541 atcgaacgag caactaaaac agatacgatc attgaaataa atgccgcaat tagtgaggca
    57601 gtaaagaaaa tgactgctgt gttattgatt tccgaatatt tttgttcgat attacttttg
    57661 acgtaaagag ccccgataac ggcatcgccg gtcggggaca ggatcggttg aacattgata
    57721 ttcacccgtt tatcgtcgtt atctaaagca acataacgtt tagtcgtgaa atcattgata
    57781 tcaacataat catttttctt tcccacagcg ctttgttcat tgacatcggt tgttcctcgg
    57841 atgatccctt tttcgtcaac gacccgtatt tcgatgatat cgctagtcgc actgttttca
    57901 ataaatcttt tgatattcgc atttacctct tcatcatctg cattatcttt tcgattcaat
    57961 tccgggctga ttgttgttgc taatgattcc accgtttgat tcatatcttt aataaatgta
    58021 ttgatcgtgg aacgttctaa accacgtata aagtaggcac caataatttc gatcgagatc
    58081 aataaaatca gaataaaggt aatagcgatc ttaaaattga ctgatcgaaa aaagcggact
    58141 tttcttttca tccaattact cctgttctgg gttacgcaaa tagtaaccga caccacgacg
    58201 tgtcactaaa taatttggat ggcttggatt atcttcgatc ttttcacgca aacgacgtac
    58261 agttacatcc actgttcgta catctccaaa ataatcataa ccccaaaccg tttgtaataa
    58321 atgttctctt gtcatcactt gtccaaggtg cttcgccaag taaaagagta gttcgaattc
    58381 acgatgtgtt aactcgattg tttctcctcg tttcgtcacc atgtaagctt ctggatggat
    58441 cgtcaaatca ccaatcgtca attccgcagg tgctgcctct tctggttcct tcgcagccgt
    58501 ggcaccgcga cgaagatttg ctttgacacg tgcaactaat tctcggttgg aaaatggctt
    58561 ggtcacgtaa tcatcggcgc ctaactctaa gcctaggact ttatcaattt cagaatcttt
    58621 ggctgttacc ataatgatcg gcatatcata agttttgcgt acctcacgag caacttctaa
    58681 gccgtccatt ttaggtagca tcaaatcaag taaaatcaaa tcaggttcta cctcagcaac
    58741 tttttccaag gcttcttccc catcatatgc agtataaacg tcatagcctt ctttggctaa
    58801 attgaatttg acgatatccg aaatcggctt ctcatcatcg acaactaaaa cttttttcat
    58861 aaagagccac ctcttatata ttattgtcat accttctcat tataccgtat ttctgctttt
    58921 gtttcatctt tgcctataaa acaaaagaga aaattaaaat gaaagtgcaa aatactttat
    58981 tattttttgt tctaacttca ataaaaaagg aaaacactga ttttcacctc agtgtttcct
    59041 ttttttgatt tatccttcta aaacttgtac accgacaccc aataatcccg gaccagtgtg
    59101 gactactaat gccggagaaa tctggccaaa gtagatttgg tttgcttgtg ggaactctct
    59161 tttaagtctc gcttccattt caattgcttc ttctttcgca tccccatggg caacagctaa
    59221 gttgaagacc tttgcattac cgattttttc tttgacgtaa gcaaccgttt tatccaaact
    59281 ttttttacgt cccctagctt ttcctactgt gtaataaatc ccttcttcat tacatgagat
    59341 gattgggtta agctttaaag cggttcctac aatagaagca actaaaccga tccgtccacc
    59401 tttttgaaga tattccaatg tcgcaacatt aaagaaaact tttgttcttt cgactaactc
    59461 attcagatta ctgatcagtt gatcccattc tactccttct tccagccatt ttgctgcttg
    59521 gatcgcagaa aaacctgcac caataccaat atttttcgta tctagtacat atgcttccaa
    59581 ttcttcttgc tgttgtgcaa ttaaacgaac cacattatat gtcccgctca atccactgga
    59641 aattgttacc gcaagtacct tttcataacc gtcttttttg atttgctcaa agatttctgt
    59701 gatcgactca ccatccggca aagatgtacc agggatttcc tctggcaaac gttgatagat
    59761 ctcttcaggg gtaatgtcaa ttttatctgt atatacctga tctttataaa taattttcaa
    59821 tgggatcata tacatctcgt atttttttat caattcttct ggaacatccg tacctgaatc
    59881 tactaaaatt gcgattttat tgtttttcat ttgttttctc ctcttttaaa tattcggttt
    59941 ctagaacaat cgttttttcc gcaagaagtt tcattgcaaa cgataaagtt gccgctctta
    60001 cagcaatata ttctacaggg gttgcctggt cgaatttttg cggatgagat ttaccttgtg
    60061 ctaattggct gaccatccac acagctgctt cttgttcatc gcaaaataga ttataggctt
    60121 cacgaattcc tactgttttt ccttggaaca atatcccttc tttgatttca ggaatagtca
    60181 atacttgttt caacgtagtg attgcaagta aaaatgctac gtgttctttg ttatatcttt
    60241 ttttaactgg tgctggtatc aaacctagtt taacgtaatt gtttaccata gaagaggtaa
    60301 gcaatggatg cttttctcct tgtatcacag gtgaaagata acgatcaact aacgttctga
    60361 cttggtccat ataaagttct aagtcaggga gttcttccca gtgaggcaga cggaatgttt
    60421 ctagttcctt cccccactcc attaattgct gacgcatttt ttccatcata accgctcctt
    60481 ttttcatttc ataaacatga tatcagactt atgataaaca ttcaatctag ttgctttaac
    60541 taggtgattt gattagcggg attccatgaa aacaaaaaac aagaaggcta agccttcttg
    60601 tcttttatcg ttaagaacag gagacccgcc ggggagacgg cgggaaagga gtaaaaaatg
    60661 aaaaaagtgt tagggtttgt tctcttgcaa gaactttgtt tgttggtatg cttatataat
    60721 agcgagcaaa tgtgaataat ctatgaatga tttcctataa ggaagtaaag atttttctga
    60781 ttttctttaa atgtagtctt gcattttaaa attcttagtg ctactattat taagtacttt
    60841 ttgtacgggc cgttagctca gttggtagag cagctgactc ttaatcagcg ggtcgcgggt
    60901 tcgagcccct cacggcccat tgggtgccaa acccacggaa caggtaaatg ttggtgcgaa
    60961 agcatttcga aaacatttac gctgttcttt ttttattttt caaaataact agggcttttt
    61021 atttttaact caaagtccac tgtagccctt tggactttga gttaaaaaag aagcataatt
    61081 atgaacagta aagtttctaa tataacaata attcgagaag ctttgcaaaa tcatgctgcc
    61141 aatgaactct ctaaacatat aggattaaat ctaagtacta taaaaggtta aaaagtgaag
    61201 aatatttaat agaaaaacta aatttacttg ctgcttt
//
LOCUS       DACDIG010000006        59186 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.006, whole genome shotgun sequence.
ACCESSION   DACDIG010000006 DACDIG010000000
VERSION     DACDIG010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59186
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.006"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            287..1198
                     /locus_tag="IV247_000445"
     CDS             287..1198
                     /locus_tag="IV247_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8776542.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(1576..2493)
                     /locus_tag="IV247_000446"
     CDS             complement(1576..2493)
                     /locus_tag="IV247_000446"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAP8776543.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            2695..3132
                     /gene="zapA"
                     /locus_tag="IV247_000447"
     CDS             2695..3132
                     /gene="zapA"
                     /locus_tag="IV247_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAP8776544.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            3190..3738
                     /locus_tag="IV247_000448"
     CDS             3190..3738
                     /locus_tag="IV247_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAP8776545.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            3853..6213
                     /locus_tag="IV247_000449"
     CDS             3853..6213
                     /locus_tag="IV247_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP8776546.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            6300..6614
                     /gene="trxA"
                     /locus_tag="IV247_000450"
     CDS             6300..6614
                     /gene="trxA"
                     /locus_tag="IV247_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAP8776547.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            6796..8574
                     /gene="uvrC"
                     /locus_tag="IV247_000451"
     CDS             6796..8574
                     /gene="uvrC"
                     /locus_tag="IV247_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAP8776548.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            8719..9618
                     /locus_tag="IV247_000452"
     CDS             8719..9618
                     /locus_tag="IV247_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8776549.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            9611..10873
                     /locus_tag="IV247_000453"
     CDS             9611..10873
                     /locus_tag="IV247_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8776550.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            11034..12932
                     /locus_tag="IV247_000454"
     CDS             11034..12932
                     /locus_tag="IV247_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAP8776551.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(13262..13981)
                     /locus_tag="IV247_000455"
     CDS             complement(13262..13981)
                     /locus_tag="IV247_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAP8776552.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            14318..14650
                     /locus_tag="IV247_000456"
     CDS             14318..14650
                     /locus_tag="IV247_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8776553.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            14637..16640
                     /locus_tag="IV247_000457"
     CDS             14637..16640
                     /locus_tag="IV247_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAP8776554.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            16659..17129
                     /locus_tag="IV247_000458"
     CDS             16659..17129
                     /locus_tag="IV247_000458"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAP8776555.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            17150..17734
                     /locus_tag="IV247_000459"
     CDS             17150..17734
                     /locus_tag="IV247_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAP8776556.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            17749..18735
                     /locus_tag="IV247_000460"
     CDS             17749..18735
                     /locus_tag="IV247_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAP8776557.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            18725..19036
                     /locus_tag="IV247_000461"
     CDS             18725..19036
                     /locus_tag="IV247_000461"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAP8776558.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            19058..20839
                     /locus_tag="IV247_000462"
     CDS             19058..20839
                     /locus_tag="IV247_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAP8776559.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            20832..22208
                     /locus_tag="IV247_000463"
     CDS             20832..22208
                     /locus_tag="IV247_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAP8776560.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            22215..22850
                     /locus_tag="IV247_000464"
     CDS             22215..22850
                     /locus_tag="IV247_000464"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAP8776561.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            22880..24235
                     /locus_tag="IV247_000465"
     CDS             22880..24235
                     /locus_tag="IV247_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAP8776562.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(24427..25821)
                     /locus_tag="IV247_000466"
     CDS             complement(24427..25821)
                     /locus_tag="IV247_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP8776563.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            25913..26884
                     /locus_tag="IV247_000467"
     CDS             25913..26884
                     /locus_tag="IV247_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAP8776564.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            26881..27663
                     /locus_tag="IV247_000468"
     CDS             26881..27663
                     /locus_tag="IV247_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAP8776565.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(27832..28203)
                     /locus_tag="IV247_000469"
     CDS             complement(27832..28203)
                     /locus_tag="IV247_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP8776566.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            28337..28780
                     /locus_tag="IV247_000470"
     CDS             28337..28780
                     /locus_tag="IV247_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP8776567.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(28823..29632)
                     /gene="yidA"
                     /locus_tag="IV247_000471"
     CDS             complement(28823..29632)
                     /gene="yidA"
                     /locus_tag="IV247_000471"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAP8776568.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(29651..31018)
                     /locus_tag="IV247_000472"
     CDS             complement(29651..31018)
                     /locus_tag="IV247_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8776569.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(31002..31832)
                     /locus_tag="IV247_000473"
     CDS             complement(31002..31832)
                     /locus_tag="IV247_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAP8776570.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            31995..32432
                     /locus_tag="IV247_000474"
     CDS             31995..32432
                     /locus_tag="IV247_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAP8776571.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            32508..33128
                     /locus_tag="IV247_000475"
     CDS             32508..33128
                     /locus_tag="IV247_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAP8776572.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            33317..33823
                     /locus_tag="IV247_000476"
     CDS             33317..33823
                     /locus_tag="IV247_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAP8776573.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            33840..34739
                     /locus_tag="IV247_000477"
     CDS             33840..34739
                     /locus_tag="IV247_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAP8776574.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            34826..35710
                     /locus_tag="IV247_000478"
     CDS             34826..35710
                     /locus_tag="IV247_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP8776575.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(35902..35973)
                     /locus_tag="IV247_000479"
     tRNA            complement(35902..35973)
                     /locus_tag="IV247_000479"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35938..35940),aa:Arg,seq:ccg)
     gene            36033..37367
                     /gene="rpoN"
                     /locus_tag="IV247_000480"
     CDS             36033..37367
                     /gene="rpoN"
                     /locus_tag="IV247_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAP8776576.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            37570..38607
                     /locus_tag="IV247_000481"
     CDS             37570..38607
                     /locus_tag="IV247_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAP8776577.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            38648..39649
                     /gene="gap"
                     /locus_tag="IV247_000482"
     CDS             38648..39649
                     /gene="gap"
                     /locus_tag="IV247_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP8776578.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            39759..40949
                     /locus_tag="IV247_000483"
     CDS             39759..40949
                     /locus_tag="IV247_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAP8776579.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            41053..41808
                     /locus_tag="IV247_000484"
     CDS             41053..41808
                     /locus_tag="IV247_000484"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAP8776580.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            41924..43222
                     /gene="eno"
                     /locus_tag="IV247_000485"
     CDS             41924..43222
                     /gene="eno"
                     /locus_tag="IV247_000485"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAP8776581.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            43622..44761
                     /locus_tag="IV247_000486"
     CDS             43622..44761
                     /locus_tag="IV247_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAP8776582.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(44828..45403)
                     /locus_tag="IV247_000487"
     CDS             complement(44828..45403)
                     /locus_tag="IV247_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8776583.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(45408..46382)
                     /locus_tag="IV247_000488"
     CDS             complement(45408..46382)
                     /locus_tag="IV247_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAP8776584.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(46406..46450)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            46665..47633
                     /locus_tag="IV247_000489"
     CDS             46665..47633
                     /locus_tag="IV247_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8776585.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            47746..48024
                     /locus_tag="IV247_000490"
     CDS             47746..48024
                     /locus_tag="IV247_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP8776586.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            48142..48378
                     /gene="secG"
                     /locus_tag="IV247_000491"
     CDS             48142..48378
                     /gene="secG"
                     /locus_tag="IV247_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAP8776587.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            48490..49236
                     /locus_tag="IV247_000492"
     CDS             48490..49236
                     /locus_tag="IV247_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP8776588.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            49239..51596
                     /gene="rnr"
                     /locus_tag="IV247_000493"
     CDS             49239..51596
                     /gene="rnr"
                     /locus_tag="IV247_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAP8776589.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWATELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            51618..52082
                     /gene="smpB"
                     /locus_tag="IV247_000494"
     CDS             51618..52082
                     /gene="smpB"
                     /locus_tag="IV247_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAP8776590.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            52384..53103
                     /gene="atpB"
                     /locus_tag="IV247_000495"
     CDS             52384..53103
                     /gene="atpB"
                     /locus_tag="IV247_000495"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAP8776591.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            53156..53371
                     /gene="atpE"
                     /locus_tag="IV247_000496"
     CDS             53156..53371
                     /gene="atpE"
                     /locus_tag="IV247_000496"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAP8776592.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            53462..53986
                     /gene="atpF"
                     /locus_tag="IV247_000497"
     CDS             53462..53986
                     /gene="atpF"
                     /locus_tag="IV247_000497"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAP8776593.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            53973..54515
                     /locus_tag="IV247_000498"
     CDS             53973..54515
                     /locus_tag="IV247_000498"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAP8776594.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            54538..56094
                     /locus_tag="IV247_000499"
     CDS             54538..56094
                     /locus_tag="IV247_000499"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAP8776595.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            56108..57010
                     /locus_tag="IV247_000500"
     CDS             56108..57010
                     /locus_tag="IV247_000500"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAP8776596.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            57033..58439
                     /gene="atpD"
                     /locus_tag="IV247_000501"
     CDS             57033..58439
                     /gene="atpD"
                     /locus_tag="IV247_000501"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAP8776597.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            58453..58875
                     /locus_tag="IV247_000502"
     CDS             58453..58875
                     /locus_tag="IV247_000502"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAP8776598.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT        19616 a        10386 c        12655 g        16529 t
ORIGIN      
        1 atgattatca gggtatattt ttatttatta tgtcggtgtt ccaggaagtg ttttaattta
       61 ataacgaaga aattaattgt ttgtagaatg aaggaaacta tttattgggt agatgaatca
      121 ccacgaatag tcaaaaggag atttcaggat atgccaaaaa tgaaaaaaat cattttagca
      181 gtaatgttat tttctacggt tatatctttt acagcccctt ttttgcttac ttaagctgat
      241 tttaagtttt ggcccttagt ctggatctac gaggtgagag tcaagaatga aaaaaataat
      301 tggagctttt gtatttgcaa tcattatcat tattatctgt tttcaaatag atgtttttgg
      361 tcaaaaaaag atagttggac aaacggtcag cagtacgaac agtacggtta caaagacaaa
      421 ggaatcattt gatgatacag aacaagagac attaatcaaa atcggaaaaa atgaccgtac
      481 actatataca gatgactcgc taactacccc acttgcaact ataaacggcg gagagctggc
      541 ggagtttctt tctgaaacaa aaaatgctta tcagatcaag acaaatgatg gctatactgg
      601 atatttagcc ctcgaagatg gaaccaaagt cacaaagaat atacaaacaa aaccggaaga
      661 gctatctgat gcagtgattg tattagatcc tgggcatggc ggaaatgata ctggggcatt
      721 aagcaatgat gaacaaacag aagaaaaaga tataacatta agtacagcca taaaagtaaa
      781 aaaggcgcta gaggatgctg gagcaacggt ttatttgacc catactacag atgaattagt
      841 acagcttgga gatatatgcg actacagtga agaaaaaaag gcagatgtct ttatcagttt
      901 acacgcagac tctacggaat atgcgaatga agcaactgga atcacgacat attattacta
      961 tggacaagag gaaacattag cacagacaat tgctgactcc ttcactgatc tgcctctgga
     1021 ttcaagagga atctccactg ggaactatca agttcttcga gaaaatcttc aaccttctat
     1081 tttaatcgag atgggttata tgaataatga tagtgattta aaggaattag taacagacag
     1141 ttatcagcag cagattgctg caagtttgac acaagcattg accacttatt ttcagtaata
     1201 aataagccca gccaaacaaa aatagctcct catatttttc gtttggccgg gcttattatc
     1261 gcagatgcaa gcttttaaac accgtttaag gaagaggtcg tgagcctgcc gttcaactct
     1321 gagtcacaag gagcaatttc aaaaaacagc ttctcatgtt tgatgactat tgtgacttgt
     1381 gaccgaggag ttggcatgag aacaccgttt aagaaaaagg ttgtgaaatc aacgttttgt
     1441 tctgagtatt agagaaacaa acggaaaaat agttcctcat attttgccgt ttgttgagct
     1501 aataccgagg atcagatgat tgaacaccgt ttattcggaa caagggactg tgacatgact
     1561 tttgtcacag ttccattatt tttttagtag cttcaaagct ttttctgtat tggcaaaatg
     1621 aagtttggca tatttttcaa gcataggcat gccgcctttc tccaatagtt ttgccgctac
     1681 ttggtcagat ttcgttccag ctcctgacgg aagggtgatt ccagcttcag ctgatgcttt
     1741 gtctaactct tccaaaaagc tggcacgact gatgatagaa gcagcagcaa cagctaaatg
     1801 atattgttct cctttagtaa cgaagtacag tttttcttgt aattgatttt tttctaaacg
     1861 cgcatatttt ttaaaatttg cttccggcgt aaactgatcg atcaaaatcg cttctggttt
     1921 ttctggctcg atttttttta gcagcaaatg gatcgcttga ttgtgcaaag cgactttcat
     1981 ccgaacggca ttgtattttg gttggatctc attgtatttt tctggagata agacaagcag
     2041 tttatagggg atggtttttt ttaagatgtc agcaagctgg atgatttggc tatcttttaa
     2101 ctcttttgaa tctctaacac caagtttacg taattctgca agctgatcac gtttcacata
     2161 agcagcacat actgttagcg gaccgaaata gctgccattt ccgacctcat cactacctaa
     2221 aacggataat tgactgatat tttttggcaa agaagaggct gtttttgctg gcttggcttg
     2281 tttatcctgt gcctcccccc atttatcagc ttctattgct gcattcgtac cttgaaacat
     2341 cacttttccg gatgtataag cggtaatggt cgtcccgtct ttttttgcag caaagctagt
     2401 ataaggaatc tgtttattta acaaataaga gcgatagtat gctttcattt gactaagttg
     2461 gtcgtttgtt gctttgataa tgacgttgct cataaaattc tcctcgtttt tatacataaa
     2521 actataccgc aactcacgac ttttcgtttt ctaagagatt gcgagacata gcattttttt
     2581 aaagtttatc atataatagt taagtatgtg aaaaatattt gtggagaacg gtgaaagaac
     2641 caacttcatg gtacactaaa taaaactttc atcaaatagg aggaaatgat tcgtatggcg
     2701 cacgaaaaaa cacgctacaa agcggtgatt gccaaccaaa catatactat tatcggacgg
     2761 gaaaccaagc atcatatgga tatcgtaacg aagctgatca atgaacagtt agcagaattg
     2821 aaacaattat caccacaaat ggataatgaa caagcagcaa tcttgatggc tgtcaatgcg
     2881 ctttcggatc aattgaagaa acaagaaaga atattggagc tggaagaaga aacagcagaa
     2941 ttaaagaaaa aaatgatcaa atttacagag cttgaaaatc gtgtgaaacg cattgaagca
     3001 atcgagaacg aagctcggga agtattgaaa gaaaatggtc aagcagatca taccattaaa
     3061 aatcatgtgg aagcacaaca gattttaaac gagaaacgaa aaggacaaat taaacaaaaa
     3121 tcttctaatt aagatccttt tatcaaaata agaaaaattt aaaaaagtta gaagagaaag
     3181 gaacgtagca tgctgagttt gctgatttta tttttattgc taattgcgtt tttttctgga
     3241 gcacgtcgcg ggtttgcttt gcaaatcata ttcgcgattg gatatgtatt gtcatttatt
     3301 gctgcccagc atttttacaa accattagca agtcatttgg aattatacat tccataccca
     3361 gctgtcaccc caacatcaaa gttagcattt tttgatcagg tatttgcctt tcacctagat
     3421 gaagcttttt atgcaggaac tgcttttctg attattttgc taattggctg gctgcttacc
     3481 cgatttgtgg gggtttttgt ccatggattg acgtatgtgc cgatcttgag acaggtagac
     3541 tggattgcag gaggcatcct gtcactaatc atggcttacg ttactatatt tttgattttg
     3601 cagttacttg cctttgttcc attagatgtg gtccaaaatc aattcggcga gcatagtttc
     3661 gctcgtttta tcgttgagag aacgccattt cttaccaaca agatgtatga gctttgggta
     3721 acgaatattt tgaattaagg aaaatagtgc tggaaaatat ttcgtttttt gtacagagta
     3781 gtttatttaa gaaggctgcg tagcgtatgt ccggcacagc tcttttttca tagtgaaaga
     3841 taaggtgata aaatgaataa ccgaatcctt gaaacattgg aatttgaaaa agtcaaacag
     3901 atggtcagac aatttgtagt gactgcacaa ggaaaagaag aactggcaga attggtacca
     3961 gtgagtgaga aacaaacgat cacaaactgg cttcaagaaa ctgaggatgg attgaaagtg
     4021 caacgtctgc gaggcgggat ccctatacct aaaattgaaa atatccgtcc ccatatgaaa
     4081 cggattgaga tcggagcaga cttgaatgga gtggagcttg cacaagtttc cagagtattg
     4141 tctacgacaa gcgaattgaa tcgtttcatt gatgatcttt ctgacagtga aatcgaattt
     4201 gcacgtttgt atatgtgggc agatcagtta gtcacgatcc ctgttttgag ccgccgttta
     4261 aaggaagcaa tcgatgaaga cggacgcgtg actgatgacg cttctcctga gttaaagagc
     4321 ataagacaga acattcggcg aagtgaacaa gctgttcgtg aacagttaga tgggatcgtc
     4381 cgtgggaaaa atgcaaagta tttaagtgat gcgatcatca ccatgcgaaa tgaccgctac
     4441 gttatcccag tcaaacaaga ataccgcggt gtatttggcg gtgtagtcca cgatcaaagt
     4501 gcttcaggtc agactttatt tattgaacca aaacaagtgg tggatttgaa taatcgtttg
     4561 cgtcaatacc aaatcgctga acgaaatgag attcaacgta ttttaagtga actttctgct
     4621 gaacttgttc cccatcgtca agaaatcatc cacaatactt atgtgattgg taaaatggat
     4681 ctgatgaatg caaaagcacg atttggtaaa gaagtaaaag ccatcgtgcc gggaatcagt
     4741 gaagacaatc atgtcgtctt gaaacaagca agacatccac tgatcgatca ggaaaaagta
     4801 gtttctaatg acatcacaat cggaaaagac tatcaagcca tcgtcattac aggacctaac
     4861 acaggtggga aaacgatcac gttgaagact ttaggtctgt tacaactgat ggggcaagca
     4921 ggattaccga ttcctgcagg tgaagaaagt caaatcggta tttttgagga agtatttgcg
     4981 gatataggag acgaacagtc gattgagcaa agtctatcga ccttttcttc tcatatgacg
     5041 aacactgtag atattttatc aaaagtcaat gaaaaaagtc tggtattatt tgacgagctc
     5101 ggtgcaggaa cagatccgca agaaggtgct gctctagcaa tcgcaatctt agatgatctt
     5161 ggtaaaaagt ctgcttatgt gatggcaaca acgcattatc ctgaattgaa agtttatgga
     5221 tataatcggg caaatacgat taatgccagc atggagtttg atgtggatac attaagtcca
     5281 acgtatcgtt tgctgattgg cgttcctgga cgaagcaatg cgtttgaaat ctctagccga
     5341 ttaggtcttg atacagaagt tattgatgaa gcgaagcagt tgatgaatga tgagagccaa
     5401 gatttaaacg aaatgatcac agatttagag aatcgccgga aaatggcaga gaccgaatat
     5461 ctggaaatgc gtcattttgt ttctgaggca caagaacttc acgatgattt gaaagaagct
     5521 tacagctatt tctttgaaga acgtgaaaaa gaaatggaaa aagcaaagaa aaaagcaaat
     5581 gaagtcgtgt cagaggcgga agaaaaagca gaaaaaatca ttgctgatat tcgtaaaatg
     5641 caacagcaga ttgggcaagg aaatgtgaaa gagcatcagt tgatcgatgc aaagacccaa
     5701 ttagcaaatc tacatcaaga agaaactttg aagaaaaata aagtattgaa gaaggcgaaa
     5761 gaacaaaaaa cgttgaaacc gggagacgaa gtgttggtta cgacttacgg ccaaagaggg
     5821 acgcttttac gtaaaaacgg gaatcagtgg caagtagaaa tcggtatatt aaaaatgaat
     5881 gtgtccgaag acgagctgac gcctgttgct cctcaaaaag agccgacaca acgagttatc
     5941 catgctgtac gatctgaatc tagtagtcac gtgccgaatc aattggacct tcgaggaaaa
     6001 cgatacgaag aagcgctaag tgaggtagat caatatttgg attctgccat tctagctggt
     6061 tatcctcaag tgactattgt ccacggaaaa ggaacaggcg cacttcgcaa gggaattact
     6121 gattatctta aaaaccatcg gagcgtcaaa agctttgagt ttgcaccggc aaaccaaggc
     6181 ggaaatggtg cgacaattgt taaatttaag taaagcttac taaaggtaag cagatggttc
     6241 gctatccaag tatagaaatg ctatactagt catagatttt aaaaatggag gtctttgata
     6301 tgtcacaagt aattacagat aagacattta atgaagaaac agataaagga cttgtattga
     6361 ttgatttttg ggcaacttgg tgtggtccat gtcgtatgca agcaccgatt ttggatcagt
     6421 tggaacaaga atatgatgaa gaagaattca gaattgctaa aatggatgtg gatgaaaatc
     6481 cagaaactcc tcaacaattc ggtatcatga gcattccaac attaatgttg aaaaaagatg
     6541 gtcaagtagt agaaaaagct gttggtgttc attcaaaaga acaactacgt caaatgatcg
     6601 atcaatattt ataaaaactt acagcaaaaa taagctgagg gtgggactga atccagttcc
     6661 gtcctcggtt tttttgcggt tgagttaagg ttattcaaat taatattttt tgagtataat
     6721 tacgttgtca ggctggattg tttttttgac agacatgcta taataacttt aattgaatat
     6781 gaaggggagt accagatgaa cgaacgaatc aaaaacaaat tagctttact tcctgatcag
     6841 cccggctgtt acttgatgaa agataaaaat ggaacgatca tttatgtcgg caaagccaaa
     6901 atattaaaaa atcgtgtccg ctcatatttt actggtagcc ataatacgaa aacggaacgg
     6961 cttgttagtg aaatcgaaga ctttgaatac atcgttacag aatcgaatat cgaagcattg
     7021 ctcttagaga tcaatctgat aaagaaaaat gatcctaaat acaacattat gttaaaggat
     7081 gataaaacct atcctttcct taagataacg aatgaaaaat atccccgttt ggtgattact
     7141 cgtaaggtac taaaagacaa agcactgtat tttggaccat atcccgatgt cggtgcggcg
     7201 aatgagacaa agaagatcct cgatcgatta tttcctctga gaaaatgcaa acctagtcag
     7261 acgaaggagc cttgtcttta ctatcatttg ggacaatgcc tgtgtcctta ttatttcgac
     7321 gtagatccga aagtctatac gaaaatcgtt gatgaagtga aaaacttttt aaacggagga
     7381 cacgaaaaaa tcgagtcaga gatccatcag aaaatggaaa aagctgctga aaacatggag
     7441 tttgaaaaag ctgcagaata ccgagaccag atccgtgcca ttgagacaat catgactcgc
     7501 caaaaaatga caaatactga tttactggat cgagatgtat ttggttatgc gattgataaa
     7561 ggatggatgt gtgtccaagt gtttttcgta agacaaggaa agctaattga acgagatgtt
     7621 tcaatgtttc ctttctataa cgaagcagaa gaagatttct tgacttacct cggtcagttt
     7681 tatcaggaaa atgagcattt cattcctaaa gaagtcttga ttcctgatac gatcgacaag
     7741 cccagtgtag aagctttgtt agctacgaaa gtgctgcaac caaaacgagg agaaaagaaa
     7801 aaattagttg aattggcgag taagaatgcc tcagttgctt tgaatgaacg gtttgatctg
     7861 attgccagaa aacaggaacg gacgatcgga gcagttgaac gtttaggaaa cgcgatgaat
     7921 atcccgaccc caatacgtat cgagtctttt gataactcta atatcatggg gacgaatcct
     7981 gtggctgcga tggtcgtatt cattgatgga aaaccggcaa gaaaagaata tcggaaatat
     8041 aaaataaaaa cagtcaacgg accggatgat tatgcatcaa tgagagaagt catctatcgc
     8101 agatattcgc gagtgattcg cgaagggttg cctttacctg atttgatctt gatcgatgga
     8161 ggaaaaggac aagtagatgt ggcaaaagat gtcttagaaa atcaattagg aatcgatatt
     8221 cctattgctg gcatggtaaa aaacgataaa cataaaacaa atgagttgct attcggtaca
     8281 gatttggctg tagtaccgct tgagcgaaat tcaccagagt ttttcttact ccagcgaatc
     8341 caagatgaag tccatcgatt cgcaatcact ttccatcggc agctacgcag taaaaacagt
     8401 tttgcttccc gattagatga aattgaaggg ttaggaccaa aacgaaaaaa aatgctgctt
     8461 aaagagttca aatcgttgaa aaatatcaca gcagccacga cagaagaatt acaagaaatc
     8521 ggactgccga aaaatgtcgc acaaaacgta tttgataaat tacatcagga atgaggaaag
     8581 ggcactttac aaagaaagta aagtgtccta tcattttatg tacaacaaaa agagcgttta
     8641 cgttttgttg gtaaataata aatcgcttac gtctggtgtc taaggatata tgtgatttct
     8701 tgaagaagga gtgaagaaat gttagaagtc aaggatcttg ttaaaacttt tgggaactac
     8761 acagctgtcg atcatgtctc tttccaaatc cccgatggaa aaatcatggg gctaattggc
     8821 caaaacgggg ctgggaagac aacgactttt cgtttgatct tggatttttt gacacaagac
     8881 caaggagaag tgttatggaa tggccatcat cttggggaga aagattatga tgtcatcggt
     8941 tatcttccgg aggaacgggg actttatccg aaagtaacga ttcaagacca actgctttat
     9001 tttgcagaac ttagagggaa atcaagaaaa gagattgaac caaaaatcga cttttggatg
     9061 gaaaaatttc aggtaaaagg gaaaaagacg gataaagtaa aatctttgtc taaaggaaat
     9121 cagcaaaaag tccagctgat cgccacgtta atccatgaac ctaaattgat cattttggat
     9181 gagccattca gcgggttaga tccggtaaat gctgaattgc taaaagatgg aatattggaa
     9241 ttgaaaaaac atggttcatg tgtcattttt tctagccaca atatggataa tgtagaaaaa
     9301 atctgcgatc atttgatcat gttgcgaaac ggaaaaatgg ttttgaatgg aaaagtacat
     9361 gagattcgtg aatcatttgg ccgtacgaag ttattcttgg aatctggttt aagccaacag
     9421 gaagtagaag aaatcgaagg cgtgaaaaaa gtgatccttc atgaagacaa tacgttagag
     9481 attaccttag aagatcctga agctgggaaa gaaatcttta ctcgtgcgac tcagttcggc
     9541 tacattccta tgtttaacca gcaaccgcca actttggaag agattttcaa attgaaagca
     9601 ggtgaaatcg atgcgtaagt tttggattat taccaaagat gtctatttaa aaaatgttaa
     9661 gtcgatttct ttcttgatca tgatattagt cccttttatc atgctgggta tcatttatgt
     9721 agcaggaaac tttgctcaac aaaatagtga tgtcaataaa atcggcgtat tggctgataa
     9781 tgagcaaatc agtcagcaaa tgagccaagt caaaacagat gattttgagt tcaaagcagt
     9841 tgattctgaa gaaaaagcac aaaaagagct ttcggaagaa aaaatcgatg cgtatttagt
     9901 agtctcaact gagaatgata agataaatgg gatgctttat agcgaaaatt cattaggaca
     9961 aagtacacaa ctactgatac aacaacaact gactggcttg caatcgatgc ttcgagcgag
    10021 tcaattaggc atttcaccag aagaagtagc tagcttgagc caacaagcaa gtttgactcg
    10081 acaaaaagtc aattttgatt caaaaggaaa aatgacagtc ggagaagata actcgacagt
    10141 ccaatacgtc gtaagttatg tagcaacgat tgtcttgttc attattattt tgacgtatgc
    10201 gcagatcatt gcacaagaga tcgcttctga aaaaggcacg cggatcatgg aagtgatcct
    10261 gtccagtacg actgcacaaa agcattttta cggaaaatta acaggtgttt tacttgttgc
    10321 attaacgcaa atggcattat acggtattat ttttggtata ggatacagtc aattcaaaga
    10381 tatggaaatg gtcaaaaata tattagacgg tatctcattg gataacttga ttggaccatt
    10441 cttgtggtat tctcttctat tcatgatctt tggcattttg attttttctg ttttagcagc
    10501 tttatgtggc tctttggtca acaaagcaga agatacagca aaagcaattt tgccagtgac
    10561 ttatctatct ctaggcggat acatgcttgg attgatacta ggagcttcag atccaaacaa
    10621 tatcgttatt cggatcactt cgtatattcc attcttatct tcatatatta tgccgattcg
    10681 cttagcaaat gagacagtcg gtatgggtgg agcattgatt tccttgttgg tacttgtagc
    10741 cgtaacattc ttattgatga tcttgtctgc aaacatgtac aaatcgaatg tactcgttta
    10801 tagcgaaggc ggattatgga gttcattgaa acaatcgatt tcaattatga ggaatgaacg
    10861 aaaaaaagca taaaacaatc ctaaaataca gaaggaagct ggacatctat tgttcccagc
    10921 ttctttttgt atagacctat tttatttatt ttactatatt ggatatatat tcattgaaaa
    10981 tagtgtataa caactttata atattgagta taccaatttt tggaggcgtt ttaatggacc
    11041 gttatttaga tttactttca gaaaaattcc cctcaacgga ggatgtcatc acggaaatca
    11101 tcaatctcga agcaattttg cagcttccaa aaggaacaga gttatttttg agtgatatac
    11161 atggcgaatt tcctgctttt gatcatattc tgcgtatcgg ttcaggaaat cttaaagaaa
    11221 agatcaaaga ccttttttct gaacaaatga cggaaacgga tatcagccaa ttcactattt
    11281 ttacggctta tccagaatat gcattaacga ctgattggta caaagaacaa gataaatcgc
    11341 agctgatcca tcatttgatc gatcttttac ggtttacgac agtcaaatat acccggtcaa
    11401 aagttcgaaa aggattgccg aaagaatata gctatatcat tgaggaactc ttgtatatcg
    11461 atgaccgtat caatggaaaa aaagactatg taaaaaaaat cattgaacaa cttgttttga
    11521 tgaaggaaga agaaaaattt ttaacgaaac ttgcacagac gatccaaaaa tctgtcatcg
    11581 atcatttgca tatcgttgga gatatttttg accgaggaac tcaagcagcc aaagtgatgg
    11641 atcgaatcat ggatttttct tctgtagata tccaatgggg aaatcatgat attttgtgga
    11701 ttggtgcgta ttgcggctct gaggcatgtt tactgacact tttacggatt gcagcacgct
    11761 ataattattt atttgaatta gagaaagaat atgggttgaa tttacgtcca ttgttttctt
    11821 ttgctcatca aacttatcag aagaatccag tatttgcgcc taaaaacagt aaaaatctat
    11881 ccgaaagaga gcaagaagaa ttagaaaaaa tccatcaggc actttctatc cttcaattca
    11941 aatcagaaca tcagttattg gatcgtcgac ctgaatttaa aatggacgat cgtaaattgc
    12001 tggaaaaaat cgactatgaa gcatcaacga ttgatttaga aggtcagctc tatgagttga
    12061 aaggaacttg ttttcaaacg atccagccag atgatccaaa aaaattcttg gaagaggaac
    12121 aaaaagtgat tgatagtttg atgtattcat tccagcactc tttgagaatg caaaaacata
    12181 tgacgttttt gatcgaaaaa gggggaatgt atctcacgaa caatcagcat gtgctgtttc
    12241 atggctgtat acctgtagac gagaagggac agctcttagc atttgaatta gaccaatctt
    12301 atcgcggaaa agaactgctg ggcttttttg aaaaacagat aactgaaagc gcaaaagatc
    12361 tgactgcaaa agaagattta gcaaccgatt tgatttggta tgcgtggagt gggccatttt
    12421 cgcctttatt cggcaagagt aaaatggcga cttttgaacg ttattttctc acagagcctc
    12481 atacgcacgt cgagaaaagc aatccttatt atcacttgcg agatgaagag tggttcagtg
    12541 aaaaaatttt agcagaattc gacacaaaag aagaagggtc agcaatcatt aatgggcaca
    12601 cgccagtaaa agtaaaaaaa ggggagtcac cgatcaaagc aaatggaaaa gtatttgtca
    12661 ttgacggagg tttatccaaa gcttatcaaa aaacgacagg aattgccggt tattcactat
    12721 tatgcaattc ttatggtttc caaatcgtga cacattatcc gtttttgcct attgaacaac
    12781 aatttgcaaa aaaatccgac caaacaaatg tgaaaaaagt aatcgaacag caactaccac
    12841 gcaaactcaa tcgtgataca acaaaaggta tggaactgca gcagcagatt acccagctca
    12901 aaaggctgtt ggactatcga aaggacgatt gagaagaatt ccagtgaaca ttgttccata
    12961 gactggagac tgagacaaaa gtcgtgtcgc agtctctttt tccggataaa cggtgttctt
    13021 ctgccaactc ctcggtcaca agtcacacta ttcaacaaac atgaaaagct gttttttgaa
    13081 attgctcctt gtgactcaga gttgaacggc aggctcacga cctcttggta taaacggtgt
    13141 tcaaaagcca gtttctcggt attaagccaa atttatgcaa aaatgtgaaa agccattttc
    13201 acaaaatttt tcttaatact cgaaactaaa cagctttttc acaaccccct cagaaagtct
    13261 attaaaattc attcacaaag aaatcgaata agcgctgttc agatggatgt acacggtggg
    13321 taagttccgg atgccattga acaccgagga ttttatgcat atcatctttg ctttgaaccg
    13381 cttcaacgag tccatcgtta gaaaaagcga tggcttttaa taaaggtgaa agatctttga
    13441 ctgcctgatg atggtaagaa ttcacaaaat atggatcggg gagaaggttg cttaacaaac
    13501 tgtctgaaac aatcttgact tcgtgagtcg taaattgcgg ggcagtgggc tgttgttcat
    13561 gttttactga ccactttgga tataatgaaa gatcttgata aagtgtacct tccaaaacga
    13621 cattcaatag ttggagtccc cggcagactg caaaaattgg ttttctttgt ttcaatgctt
    13681 ccaagatcag tgcctgttca aattggtctc gttgaatatt tgtttccgtt agctttggat
    13741 gaggatcttc catgtaaagt tgtggactga catcttgacc acctgcaaga ataagtttgt
    13801 cgatttgtcc aatcagttga ggagctgaat ctggagacat gactggcaga ataagaggca
    13861 atccttgagc atctaaaact gcatccacga atccttgcgg tgtatagctc acttgatttc
    13921 cttggaaagt atctgttgct cgaatcagtt gatttccggc aatgccgatg attggtttca
    13981 tttgtattcc ccctaaagta gacttattgt aacaaggatt taaaaaaaat taaactatgt
    14041 aagacttgcc cctatactta tctgttcttg ctgtgttact atctgtaaag agaaatttcg
    14101 tgtgaaattc atgacgatag ttgaaaatat tgcttaattt gttataataa aaagtaagca
    14161 gaaataaaaa ggttgggcgt aagcttcaag gatctagttc ttcaaggggt gagaaggaca
    14221 ttttattgaa ggggcctgcc tatactaagt tatctttaga ggtacatact ttcaatgaaa
    14281 aaagaaagca tgtacctctt ttttaaatcg aaaggaagtg aagcaagtgt tagaagcttt
    14341 ggaacaaatc cgaaaagcag aagaagaaaa tgaaaagcgt gctcaaacac tgcggcagca
    14401 gctgaagatc tatgcaacag aaaaagagca ggaattggaa acgatccaaa caaactatcg
    14461 acaacaacta aaacagttgg tggaagaaaa agaacaattt gaaatacaag aattagaaaa
    14521 agaaaaaatg aaattgaaag cagaagcaga caaggtgcaa gcaactttga aagatcaata
    14581 tcaaaagcat cagagtcaag cgatcggtgc gatcattgaa agggtgaaag aaacttatgg
    14641 ccgtcactaa gatggaaaaa gtgaccctca tttcagataa aagaaatcag gagaggattt
    14701 tacaagcagt acaaggaatc caaaacgttg aaatccggga tctgtatcaa aatagaacaa
    14761 acaaccagtg ggtggagaag tattttccag ataccacgat tattgataaa gaagcaaaat
    14821 taagcgcttt aggtaatcgg ttgaaagaca tccaagaagc tatccaattc atacaacacc
    14881 acggcgatag ccgtcaaaag aaactccatt tgaaacgtca agaactttcc ttacaagatt
    14941 tggaagccaa ttattccgaa gaaaatttca taaataaatt gaaagaagtc cttgatttaa
    15001 aagaaaagtg ggaacaacta actgaagaac gtgatcgttt agaagaagag gaagaatggc
    15061 ttcttcattg gcaacagctg gatattgctc caagtatgta tgcaagtaag acgaccattt
    15121 ttgagatggg gactgtgagt gcagcaaatt ttgaaagttt caaggaagaa ctgagtaagc
    15181 ttgatgacgt ttatatggaa gaaatagatt atacgccaaa acaagtttat ctttcgtatg
    15241 ttgctttaaa aaaatcagtt gctgttatag aagaaattgc tgggaaatac gggttcaata
    15301 aagaaacgta tccttatgaa caaaatccta aagaaaaact gcaagagacc aagcagcaat
    15361 tatccattgt tcacgagcag caaaagaaga taacgacagc gttaggtcag tgcagtggct
    15421 atatccaaga atttgaatgg gtagaagaag tcacacttgc cgttgcagaa cgagaaaaaa
    15481 taaaagaacg gtttatccat gcgacttatc tgatcgtttt gcaaggttgg gtagatgcag
    15541 aagaaaaaca gaatttgctg catgtgttaa acgaaaccgt atcagaagaa gatatatacg
    15601 tcagtttcga aacaccaaac tcagaagaga ttcagcagga agtccctact aaattgaaaa
    15661 accatccgct tgtcgaacct tttgaattgc tgactgaaat gtacagtctg ccaaaatatg
    15721 aagaagtaga tccaacacct tggatgacac cattttatct tgtcttcttc gggatgatgg
    15781 tcgcagatgt tggttatggt ttgctgatgc tcattgggac aatactagct caaaaactac
    15841 ttgttctacc acgcgggatg aaacgtttcg taaaattctt tgagattctc tcgataccat
    15901 cgatcatctg gggattgatc tacagttcat ttttcggaca gtcattacca aaagaactgt
    15961 ttggcatccg cttgcctttc ccaatcctat ctacgacgga tgacgtcaat acgattttga
    16021 ttttatccgt gattttcggt ctgatacaga ttttggttgg attatttgtg gcagcaaagg
    16081 aaaatatcag gagaaaagat tacttgagtg ctgtcagtga tggatttgcc tggcaaggga
    16141 tcctaattgg gatcgttatt gcgttggttg gtgccatgtt gataaaaaat acagcatttg
    16201 tttaccttgg cgctgggcta tcgatcattg ctgccttatg tatcttgatc gttccgatta
    16261 tccaaacgcc gtctaaagtc aaaggtgctg ccaaaggtgc gtataatctt tacggactga
    16321 ccggctatat cggtgattta gtcagttata cacgtttgat ggcattgggt atttcaggcg
    16381 gaagtattgg agcggcattc aatatgctag ttgctttcat gccgccagct gctcgattca
    16441 gtgtagggat ccttttgatc atcgctttac acgcactgaa tatgtttttg acactgctaa
    16501 gtgcctatgt ccatggcgca cgattgcagt atgtcgaatt ttttgggaag ttttatacag
    16561 gaggcggacg tgcctttgat ccgctgaaaa cggcggaaaa atacgtcaac atcaatcaca
    16621 agaaaaaaaa caataaataa ttggaggatt tttatataat gatggattac ttaattagtc
    16681 aaaatggtgg aatggtattt gcggtattag cgatggcaac agcgacgatt ttttcaggga
    16741 tcggttcagc taaaggtgtt ggaatgacag gtgaggcagc agcagcattg acaacaagtc
    16801 aacccgaaaa attcgggcag gctctgattc tgcaattgct tccaggcacg caaggtttgt
    16861 atggattcgt tattgccttt ttgattttta tcaacttagg aaatgacatg tcagttgtac
    16921 aaggactgaa ctttctaggt gcttctctgc cgattgcttt tacaggcttg ttttcaggta
    16981 ttgcccaagg aaaagtcgca gcggcaggta ttcaaatttt agcgaaaaaa cctgaacatg
    17041 ctacaaaagg aatcattttt gctgcgatgg ttgaaacata cgcgatttta ggtttcgtta
    17101 tttccttcct attagtgttg aatgcctaag cataaaggag gaaaagcaga tgaacgccat
    17161 tgaaaaaatc atctctcaga tgaacgaagc agcagagcag gaaagagcgg cgttagaaca
    17221 agaagagcga atgaagatcg atcaaaactt tgaacaaaaa cgtacgcaag ttgaaacaga
    17281 acatcaaaaa caaaaagaaa aacaaatcga attactcgaa aaaaaatacc gtcagctaag
    17341 gaatcgccag caagtagaag tacgacaaga aaatttgaat gcgaagcagg aatttttaag
    17401 acgattgttt gcagatgcgg taactgaaat ggaaaattgg gatgaatctg aacaaatcca
    17461 gtttatcaaa aatgcattgt attcattacc tttgacagga aaagtagcat tcattgctgg
    17521 agaaaaatca gcagcctatc tttctcagac gttgttggat gaatggaaca atgaacttcc
    17581 ctttatgatg gttttaagtg atgaaactgt tgctgatcaa gcgggttttc tgataaacga
    17641 tcaaggagtt cagtataatt ttcttttcag cagccttgta caagatatcc aaggaacgat
    17701 gagttttgag attgccaatc aattattcga ataaggaggg atcagtgtat ggaataccac
    17761 gaactaaatc ccttgattcg cggaagagag cttgaacttt tgtccaatga gacatttgaa
    17821 cggatgattc aaacaaattc tgtcgaagca cttggtgaaa tattgaaatc cacgatttac
    17881 agtccatata tttatgacgg atatgaatat gacttcgaac agaatctttt aaaggagcag
    17941 agccgtctat ttcgctggtt aaaggaatca gcaccggagc ctgaaatcgt ttggatctat
    18001 acgatgcgtt atactttcca taatctgaag gttttgacaa aagctgagat gacaaatcaa
    18061 aatctggacc atttgtatat cgacgatgga ttttattcat tagaaacctt gaaggatgcc
    18121 attcatacgc aagtttcaaa tgaacttcct gaaagcatta tggacagtat acgagaagta
    18181 cgtgattatt ttgaagagtc taccattctt cagggaattg acgtgattta tgatcgacag
    18241 tttttgacag agcagcgacg gcttgccgat aagctaggat atcaagaatt gctgacagaa
    18301 gtcattgctt ttattgactt gaccaatatc acaacgatgg ctcgaggaat attgcagaaa
    18361 agaacatatg ggttcatgac gacggtactt tccagctcgg gaagtatcga aaaagaagta
    18421 tttttgaact ttgtagataa agatatggaa gattacacga attttctatt agagacagat
    18481 tatgctgatt ttctgcgccc tgtcatccgt gagggaaata ttgatctcgt aaagcttgaa
    18541 cagttgaaag atgattatct aagttcattg tatcaagaag cacaaaccca ggctttcgga
    18601 ccactgccac tactagcctt tttgaacgca aaagaggtag aaagcaaaaa ccttcgttta
    18661 ctggttatcg gcaaaagaag ccatttcgaa tcagaaacaa tcagaaagcg ggtgagacaa
    18721 gtctatgacg cataagatcg gtgtagttgg cgacaaagat tctgttttgc cattcaaatt
    18781 gtttggcttt gatgttcgtt atggtacaac caagcaaacc gttcgtcagg caattgaaga
    18841 aatggcaaag aatgagtatg gcgtgattta tgtcacagag caatgtgcag cattgagccc
    18901 agaaacgatc gaacgttaca aggaacaaat gatacctgct atcgtattga ttcccaatca
    18961 tcaaggatca ttagggatag ggctggaaga aattcaaaaa aacgtagaaa aagcagtagg
    19021 tcaaaatatc ttatagtatg aaaaggagat agaaattttg caaattggaa aaatcataaa
    19081 agtctccgga cctcttgtaa tggctgagaa catgtctgat gccagtatcc aagacatgtg
    19141 tctagtggga gatctaggag ttatcggaga aattatcgag atgcgtggcg atgtggcgtc
    19201 tattcaagta tatgaagaga cttctggaat tggtccaggc gaaccggttc gctctactgg
    19261 tgaagcttta tctgttgaat taggaccagg gatcatttca caaatgtttg acggaatcca
    19321 acggccatta gataccttta tggaaatcac acaaagtaat ttcttagggc gcggagttca
    19381 attgcccgca ttggaccatg agaaaaaatg gtggttcgaa ccaacagtag aagccggaga
    19441 aacggtgagt gctggagatg ttatcgggat cgtagaagaa acaaaagtta taaaacataa
    19501 aatcatggtg ccaaatggga taaaaggaac gatcaaaaca atcgaaaaag gttcattcac
    19561 tattgatgaa acagtatgtg tcattgaaac cgaaaatggt gacaaaaatt tgacgatgct
    19621 tcaaaaatgg ccagtaagac gtagtcggcc aatcaaagaa aaattaaacc cggatgcacc
    19681 catgatcact ggacagcgag tgattgatac attcttccca gtgacaaaag gaggagcagc
    19741 agctgtacct ggtccttttg gtgcagggaa aacagtcgtt cagcatcaaa ttgcaaaatg
    19801 ggccgatgtg gacatggttg tctatgtggg atgtggcgag cgtggaaatg agatgacgga
    19861 tgttttaaat gaattccctg aattgattga ccctaataca ggagaatcgt tgatggaacg
    19921 tacggtattg attgctaata catcaaacat gccagtagca gctcgagaag cttctatcta
    19981 tacgggaatc acaattgcgg aatatttccg tgacatgggt tatgacgttg ctatcatggc
    20041 agactccact tcacgatggg cagaagcact tcgtgagatg agcggacgtt tggaagaaat
    20101 gcctggtgat gaaggttatc cagcttatct tggttcacgg cttgcggaat actatgaacg
    20161 atctggaaga gtcattgcat taggatcaga aggtcgcgaa ggaagtataa ccgcaatcag
    20221 tgctgtatcc ccgtctggag gggatatttc ggaaccagtc acacaaaata cattgcgggt
    20281 cgtaaaagta ttctggggtc ttgattccag tttagcccaa aaaagacatt ttccttcgat
    20341 cgattggata caaagctatt ctctttattc aacagaagta gggaagtata tggatcatat
    20401 ccttcaagct gactgggcat ccatggtcac cgagggcatg cgtattttgc aggaagaaca
    20461 gcagctgaat gaaatcgtgc gtttggtagg gattgattcc ctttcagata atgaccgtct
    20521 gacattagaa gtggcaaaat ccattcgtga agattatttg cagcaaaatg cttttgatga
    20581 tgtcgataca ttcacttcaa gagagaaaca attcaaaatg ttgaagatga tcttgacctt
    20641 tggtgaagaa ggacgccgtg cgttgtcgtt aggtgcttat ttaagtgaga tcatggacgg
    20701 taccgtatcg attcgtgaaa ggatcggacg aagcaaatat atcccggaaa cagaattaga
    20761 aaaactagat caaattattg gtgaaatcaa gcaaacgatg caaacgatcg tttcagaagg
    20821 aggcatgagc aatgattaaa gaatatcgta cgatcggtga agtcgtcggt ccattgatgg
    20881 cggttgaaaa agttgctggt gtcaaatatg aagaattaat cgaagtccgt atgcaaaatg
    20941 gcgagattcg tcgtggacaa gtattggaag tccaagaaga taaagcaatg gtccaaatat
    21001 tcgaaggaac aagtggtatc aatttacgtg actcttctgt acgattttta ggccatcctt
    21061 tagaactagg tgtatcggaa gacatgatcg gtcgagtatt cgatggtttg ggacgaccga
    21121 aagataacgg accagagatc ctgcctgaaa agcatttgga tattaacgga gaagtaatca
    21181 atccagtagc tcgggattat ccagatgagt ttatccaaac aggtatctct gcaatcgatc
    21241 atttgaatac attagttcgt gggcaaaaat taccagtatt ttctggctct ggtcttcctc
    21301 ataaagaatt agctgctcag atcgcgcgac aagcaacggt actcgattca tcagatgatt
    21361 tcgccgtagt ctttgctgct attggaatca cttttgaaga agctgaattt ttcatggaag
    21421 attttcgtca aacgggcgca atcgaccgtt ctgtcatgtt tatgaattta gcaaacgacc
    21481 ctgcaatcga acgtatcgct actcctcgta tggctttgac tgctgcagaa tatcttgctt
    21541 atgaaaaggg aatgcatgtt ttggttatca tgacagacat gaccaactat gcagaggcat
    21601 tgcgagagat ttctgcagca cggcgagaag ttccaggaag aagaggatat cctgggtatc
    21661 tttacacgaa cttagctact ttattcgaac gagcaggaag aatccgcgga ctaaaaggat
    21721 cggttactca aattccgatt ctaacaatgc cagaagacga taaaactcat ccaatccctg
    21781 acttgactgg ttatattaca gaaggacaga tcatcttgac tagagagctt tacaagagtg
    21841 ggattcagcc accaattgat gtactcccgt ctctttcgcg gttaaaagat aagggaactg
    21901 gacccggaaa gacaagagaa gaccatgcag ctacgatgaa tcagcttttt gcagcctatg
    21961 cacaaggaaa acaggcaaaa gaattagccg tagttttagg tgaatctgct ttatctgatg
    22021 tagacaagat ctatgcgaag tttgctgagc ggtttgaaaa agaatatgtc aatcaaggat
    22081 tctacacgaa ccgttcgatt actgaaacac ttgatctagg gtgggaactg ctatcgatgt
    22141 tgccaagaac cgaattaaaa cgaatcaaag acgacatgct ggacaaatat ctgccagaag
    22201 ggaagtgagt tctgatggca cgattgaatg tgaatccgac ccgcatggaa ttgactcgat
    22261 tgaaaaaaca gttgacaact gccacaagag ggcataaatt attaaaagat aagcaagatg
    22321 aactgatgcg tcaattcatc ctgttgattc gaaagaacaa ccagctgcgt caagaaatgg
    22381 aagagtctat gacagaagca atgtctgatt ttgtattggc aaatgcttcg atcacggaac
    22441 ctttcatcga agaattattt gttttgccgg cggaaaacgt tgatttgtct atcgtagaca
    22501 aaaatatcat gagtgtaaaa gtacctgtga tgaattttga atatgatgaa cagctgacag
    22561 acacaccttt ggaatacggg tatttgaatt ccagtgccga actggatcgg tcaatcgatc
    22621 ggtttactac cctgcttcca aaacttttgg aactgacaga aatcgaaaaa acttgtcaac
    22681 tgatggcgag tgaaatcgaa aagacacgtc ggcgagtcaa tgcattagaa tatatgacga
    22741 tccctcaatt agaagaaacg atctattata ttcgtatgaa gctggaagaa aacgagcgcg
    22801 cagaagtaac aaggttgatc aaagtaaaaa atatgggtac cactgactaa gtggtatctt
    22861 tttttaaaag aggaaaacga tgattaaaca aagcaaacaa ataaagaaac gtttgccggc
    22921 tgttcagctt ctcgcattag gattttttct cctgatttta gttggcggcg gcctacttac
    22981 cctaccattt tttagtaata gtgggcatgg gacaaatttt atcgatgcgc ttttcactgc
    23041 aacttctgct gtctgtgtaa cgggattgac aacattgaat acagcagagc attggaacag
    23101 tgctggtcaa tttttgatca tgcttttgat tgagatcggc ggtcttggtt ttatgatggt
    23161 tccgattcta tgctttgcat tgataaagaa gaaagtcagt ctcagcacac gtattatatt
    23221 aaaagaagcg ctgaatctag aagaagtatc aggtgtgatg aagctcatgc tttacatcct
    23281 aaaattggcc tttgtgatcc aattgattgg tacggttgct ttatcttttg tatttgtgcc
    23341 tgaatttggc tggttgaaag gtatctggta cagtttgttc catgcaatct ctagtttttg
    23401 taatgcaggg tttgatttgt taggagacag tttagccaat caccagcaaa atgtttattt
    23461 gatcatggtg gtatctgctt tgatcatttc tggtgggtta ggcttcatcg tatggcgtga
    23521 tttactggaa tatcatcaga aaaagaagat gacgattcat tcaaaaatcg cattaaccgt
    23581 tacaggtacc cttttattag gcggattcgt tgtgttcttt ttcacagagc gcaatggggc
    23641 aacgctagtg gaagggaata tgtttcaacg attggcaaac acctttttta tgagtgtgac
    23701 acctagaacg gctggatatt attcagttga ttacctccat atgagccatg caggtctgat
    23761 tttaacgatg tttttgatgt acatcggcgg aacatctggg tcgacggccg gcggattgaa
    23821 aacaactacg ttaggtgtgt tgctgatcca gatgcgttcg atgttgaaag gacgtacgag
    23881 agcggaagca tttggccgta ccatccgaca aggcgcagtg ttgcgggcac ttactttatt
    23941 ttttgttacg ttgacgttgt gtgtagtcgc catcatgatt ttatcggtga ctgaaacgat
    24001 acctaaaact tctggcatcg aatatattgc ttttgaggtc ttctctgcgt ttggtacagt
    24061 aggtcttacg atgggattga cgccagactt gactgcaata gggaaattga tcattatagc
    24121 gcttatgtac attggacgag ttggtgtatt gactgtggtc ttctctctga tgacaaaagc
    24181 acaaagaaca caatcaaaat acaaatatcc agaagaaagt gtcatgattg gataatttca
    24241 tcttctgaca gaaacctagt gatttagtaa aataagaaaa taaaataatt tttcttgctt
    24301 cttggatact ttaagtgcaa aatgttcgtt tattttttga ggcactaccc gttgttgaac
    24361 ctcgggtagt gcctcttttt tattgaaagg aatcaataag atttgattcc tttatttatc
    24421 aataggttat aattcatatt tttttatttt ttcttttacg atacttagtc cagaagctaa
    24481 aatttcaagg ttattagagg atagggaaat tcgaacgaat gcatctggtt gcgcacgtgt
    24541 agtaaagcgt ctggaatgat aaagtcggat accgttagca agaaagtcat tctcaattat
    24601 cttagggtca atctcaggat caactggaag aattcgatag taagggacac gattcgttgg
    24661 tttttctact ttgaaaagag aatcaaaaag ctgattggct ctttttgtaa gacttaattt
    24721 agtttctaag atcctatttg cttcatttga atggaggatt tgagtgatga cttccacatc
    24781 taatccagaa gttttcacat ttatattaaa aattgcttgt tctatcttgc tttggacatg
    24841 tactggaact acaagataag caactcgcaa tccagaacaa ataaattttg tcattccagc
    24901 taagtagatt gtttggttag gtaataaatt ttggaaagga ggaatgatcg tatgttgata
    24961 gtaagttgtt aaaaatgaat ggatatcatc ttcaataacc catagacctt ctttttcaat
    25021 cacttcggct aattgctccc gtcttttgtg agagatttga aaccctatag gattattaca
    25081 agaaggcata agaaagacac catgaatctt cttttttcta cattcctgaa tcaatatatc
    25141 aggtcgcatt ccttgctcgt caaaatcaat tggaacgatc tctaaatgat ataactgagc
    25201 caattcaatg aaattagcgt atgtatatcg gtctgttgct attctatcac ctggagagaa
    25261 aagtgcagaa agtgtgatag ccaatccatt ttgaacaccg gaaacaatag atagagattc
    25321 taaggacgga atgactcctt gagtttttaa ccaatttgct gcagtagtca attgaaaatt
    25381 tgtgcctttg ggatcttggt aattaagcaa accaacaaga tttgggttat tactagttga
    25441 ttgaataaac gggacaacga atttattaca ttcttcaaaa gaactaacca gaccaaaatc
    25501 aatgatatgt tcggttactt cattggtaga gatggtgatg gactcaaaga cattgggaga
    25561 aataaatgtg ccacttccaa tattcgtata cagcaaacct ttctcaattc catatttata
    25621 agccttgcca acggtagtaa aatttaaatc taaataatca gctagctctc tttgggaggg
    25681 taactgagtg tttttctgta atttattctt taaaatatct tgttctaatt gttgaatcaa
    25741 cgatcgataa taaggacgct tcaatttttg tttgtctggt cgccaagtta acgggtaatt
    25801 gtcataagaa ttaataggca ttttctccac tccttaaaat tgtataccat acaattttac
    25861 cattgattgt attgatttta gaagttaatc ttttgtgagg agggaattgt ctatgaaaat
    25921 tattaatttt gaggaagtag tagcttcttt tagttttgaa gaagcgattc aagtaatgaa
    25981 aaaatgtttt tttgattatg aaagtggtaa aatcagccaa aatcctcgat tggtggaaat
    26041 tttacctgac ggaaaaggca aaaatatgtt tgcgatgatg cctgcttatt tgggggagaa
    26101 cagatatttt ggcacaaaag tcattaccgc ttttccggat aatcatcagt atcatttacc
    26161 ttctcattta ggagagattt tcttgtttga tgctcaaaac ggactaccca aagcattgat
    26221 aaacgcaaat gcagtcacct ggattcgaac agctgctgta tctgcgctgg caacagatta
    26281 cttagcaaat ccgcatgcag aaacacttgc tttaattgga gctggtcaac aagcattgtc
    26341 gcatttgact gcgatttcta ccattcgtcc catcactaca gtgtttgttt acgatatttt
    26401 agaagaaaga cgaaatactt ttatcgctaa agctaaaaaa cagtatccta aaataacgtt
    26461 tataaatgca atatcagtag aagatgcagt acgtccagcg gaaatcattt gtacgttgac
    26521 ctctagtaaa gaggcatttc ttcaaaaaga gtgggtaagg gaagacgcac atatcaatgc
    26581 tgtgggcaca tttactcctg atacaagaga gataagtaca tcgttgatgg aaacttctca
    26641 aatctatgta gatgattact cagctgcctt aacagaaagc ggggacctgc ttatagccat
    26701 ggcagaagga gatttagagg aatcttgtat tgttggtaca ttaggcgaat tagtttctga
    26761 gaaaaaaaca ttttataaag gagaatcgag gccgacgatt ttcgacgcag ttggtctagc
    26821 tattgaagat ctttgttgtg cagaatatat ttataacaaa ttagagaaag gggctttaaa
    26881 atgaacttag attattacgt agttgatgca tttgcagatg aggtgttcaa aggaaatcca
    26941 gcagcggttt atgtgctaga agaatggctg ccagaaggta cgatgcaaaa aattgccatt
    27001 gaaaacaatt tatctgaaac agcatttaca gtgaagaaaa accaagaatt tgagcttaga
    27061 tggtttacgc cagatcgaga aatcgatctt tgtggtcacg cgacattagc aacagcattt
    27121 gttttattta actattacaa aatacctgat gaaacgatta aattttctac tcaaagcggt
    27181 aatctatttg tgacaagaat taaagattat tattacatgg attttcctag tattatgccg
    27241 aaaaaagtgc cgatattgtc tgaatatgaa gaagcgattg gggctaagat taaagaagcc
    27301 tacttagcac gtgatttatt tttcgtatta gaagatgaaa aaacggtggc taaattgcag
    27361 ccagatttta cagctatcaa aaattttgaa ttaggaatag gagtcatcgt gacagcagaa
    27421 agtgtgcaaa aagactttgt ttctagaacc ttctttccaa aattaacaat caatgaagat
    27481 ccagtatgtg gatcagcaca ttctaatctt attccttatt gggcagaaag aatgaataaa
    27541 acaaaattgt cagcttatca aatgtcatca aggggcgggg atctagaatg tgaattaaaa
    27601 gaagatcgtg tgataatagg aggaaaagca gttttgtttt ctaaaggtac ctcgtttatt
    27661 tgatttcttt caaatgaatt aaaaataaat tggtggagag tggcaacctt atagctacaa
    27721 tggttgttaa aaaaagacgc aaactttttc aatttaatga aaagtttgcg tctcatttta
    27781 attattggtg tttgtttttt attcaatcaa gagtaaaatg ccccatacta actatcctcg
    27841 gatgacttcg attttataac catcagggtc aatgacaaag tagtaagaag gcgcagtgcc
    27901 aggtaatcct tttaagtcag taatctcaag accagcagct ttatgttgct cgtgtaattt
    27961 ttctagatca tcagctgcta tagcgatatg accatagccg tctcctagat catacgcacc
    28021 atgatcatag ttgtaagtca actctaattc atagtcatcc ccaggaagtg tcaaataaac
    28081 aagtgtaaat ttgttttctg gaaaatcacg gcggcggctt tcttcaaaac caaatgcctt
    28141 ttgataaaat tcgattgatg cttcaagatc tttcactcga acacatgtgt gagccatttt
    28201 catagattca tctctccttt tccttttact caatcataac aaaaagaagt aaggaaatag
    28261 ttgatttgtg cttgttcatg agaaatttct catgtaaaat ggatagaaaa taaaactgtt
    28321 aaggtggaaa ctattcatgg ataaaccaat ttttggtgaa aaaaagcaat ttgctgatta
    28381 tcaaacccgt tatgcagcat atattgtcat agaaagagat cagcagattg cagtaatcga
    28441 ggctccaaat ggtgcttttt ttcttcctgg cggggaaatt gaaggagaag agacgaaaga
    28501 agaagcgatt gtccgtgagc tgatagaaga aatgggaatt gtggcagaaa tttcttctta
    28561 tctgggagaa gctgatgagt atttttactc gaattatcgt gcaacttatt attataatcc
    28621 aggatatttc tttgttgctg attcttggaa gcgtatagga gaaccaactg aaaagaccaa
    28681 taaaatttgg tgggtgagcc ctcaagaagc gcttgtgaaa ttgaaacgtg gtagccaccg
    28741 atgggcagtc gagagatgga tcgaaaaaaa taaaaactga aagaaagcga gtagatcagc
    28801 tttctctcag tttttttatt tattacagta catatttttt gattgcttca gctacaccgt
    28861 gctcgtcgtt tgatgaagtg atgacatttg ctgcttcttt gacaagagaa acggcatttc
    28921 ccattgctac tcctagacca gcatattcaa tcatggataa gtcgttctcg ttatctccga
    28981 tagccatgat ttcatcttga tcaataccaa ggtgctgtgc gagattcgca actgccgcac
    29041 ctttacttgc ttctttattt agtacttcga agtaataggg cgcacttttg accgttgtgt
    29101 atttatcacg gaaagtttgc ggtaaacgcg caattgctgc atctaaaatt tctggttcgt
    29161 cgatcatcat cattttgacg atgttcatgt ctggtgtcat ttcttcaggt gttcgatatt
    29221 ttagcggcat cttgactaaa cttgcttcgt ggatcgtata agcgctgata tctcgattag
    29281 gcgtgtagat cgctttgtca tcgatcgaat ggagatgact gccgacacgg cgtgccatta
    29341 cttcgatctc taggaaatct tcatgtgtca gtccgtgacg ggcgatgatt ttacctgatt
    29401 tcgtttgttg tacgagtgcc ccgttataag taataacgta atcattatct tgaaaaaggt
    29461 tcaactcgtt taactgttcc tggacacctg gtaagggacg gccagtgcat aaaacgatcg
    29521 aaacaccttt tgcaacagca gcggcaatcg cttcttttac ttcttttgtt acttctcttt
    29581 tctcatttaa taaagtacca tcgatgtcga tagcgactag tttgatagac ataatttcct
    29641 ccaatatatt ttaatttatt tcaatcaatg cgccattacg gatatggctg gcaaattctt
    29701 gataagtttc gtcaaataaa tcataatgat cacgcaagga cggatcaacc atttcttttg
    29761 gaaagaaaaa tcgctcatct ccttgttctt gtcccgctaa tgcagcaaca agctgactaa
    29821 cctgtgataa ttctatcaaa gttccgtcat ttcgcaaaat ctcgatttgt gtgcgatgtc
    29881 tgccttgttc tgggcgataa aaatcataag gtagatcgta gctagaattc acagcagtgt
    29941 aataaacagg attatatccg accttttcta ctaacaaagt gagttcttgg actaattttg
    30001 aatcccggtt gccagaaaaa gtagcagatt tcagtggttt acgattcaaa aaacgtttag
    30061 ctaaatcatt taaaatagga tcagcagatt cagaccattg agtaaagtaa gtgcccaaga
    30121 ccccatcatc aagtttgagg tattcttcta aagtaaagct tccgtcaaga aatggcagaa
    30181 gtaattgaga atggagttca aaactgctgg catcttcttg atacaactcg tgtgcgcgat
    30241 gaagcagatg ctccaaaatt acttccatac cacgggaaac aggatggaag tacacttgca
    30301 catacatttg atatcggcta acgatataat cttccactgc atgcatgcca ctcatggaaa
    30361 aagcgatccc gccttcataa ggacgaatca cacgtaatat tcgggtaaga tcaaatgttc
    30421 catattctgt tccagtgaaa taagcatccc gaagcaaata gtccattcga tctgcatcga
    30481 tctgactaga aatcatttgg acaacttgtg ggttggggta agttttttgg atcacactag
    30541 ctaccttttc aggaaaacct tcttctactc ggttaagaat ctgatacact tctgtttctg
    30601 gagaagtgat gatctctaca gtgatggctt catgattcgt atggaagata tgttcaaatg
    30661 tatgagagta aggaccatga ccgacatcat gcaacagtgc cgcacataaa gtcaccagcc
    30721 gttcctgatc atcccagcct ccgttgccta ttttttcttt tgaaaaattt cgagaaaaga
    30781 tatcgcagat tcgtcttgaa atctcataaa cgcctaatga atgagtaaaa cggctatgtt
    30841 ctgctccgtg aaaagtgaaa gaagaagtgc ctaattgttt aatgcgtcgt agacgttgga
    30901 cttctttcga attgatcaaa tctaagatta cttgatgctg gacatggata tagttatgga
    30961 ctggatcacg aaaaactttt tccataggca gcatttgata tttataaggg gttgtcataa
    31021 tgtagctcct ttatgttatt acgttgttcc attctctgga tttggttgtt ccattgctcc
    31081 agctggattt ctttttttag ccagtcggta gaatcctgta aatcacttgc agaatacagc
    31141 ttttcaaaag cttttgcaaa tgcttctttg acttctttta caaaaagggg ttggtcaagc
    31201 agttcttcaa gtgttgccat tgtagcagga tccactgcag gatagccatt tgtaccaaag
    31261 tcttctttta aagcagattg ataaaatcgc ttcatgactt gcccacgttt ttcctgatta
    31321 ccgttgacac ttaagtacat cataacagaa atcccatttt tgatccgacg ctgagcgata
    31381 ccggcaaatt ttttgccatg gatgcttaaa tcataagttc caggacaata agaatgggtg
    31441 atttcgaatg cttgtatcgg tgcctttgga aaagcgtatt tagtcaatag gagcatttgc
    31501 tcgtaagcgt catctatggt tatttttttt gtgtcacttt gggggaaaat caaagaaata
    31561 ttcaaaattc cttcatctga tacgatccct aatccacctg aatttctgat cactggagcg
    31621 taacctgcat cttccagtgt ttggatcccg tcacttagaa aaggagttcg aacatccttc
    31681 atacctaaga tgactgtgca gtcatattgc cagaaatgta aaaagatttg ctggtgttct
    31741 ttagaatatt ccgttaatat atctgttaat gcaaaagggg aaaagtcgtt tttttgataa
    31801 atgtgctgat ctagtcgaat cgcagggttc attcaaccgg ctcctttgta taaattcata
    31861 ttttctacaa tattatactc taaaatcaaa gagttgtttt ggaaaaaagg ttttctgtaa
    31921 aaatggacaa gttggttgac aaaacaaaag aaactaggaa caatgtagct atgagaagta
    31981 aaggagaatg aataatggaa ttatcacaag gaacaccgat ttcaatcaaa ttgcgtacaa
    32041 aagtaaaaca gcaaggggaa gtgcaagatt tttattttga tttgaaaggg caaatggtga
    32101 caattggcga tacgctgtac atacgttata aagagattca ggaggagacg ggcgatgaga
    32161 ttcctgtaac gatcaaatta gttccagatg gtcatgtaca gctgatccgt gcaggagaaa
    32221 tgcggatgcg tttgaaattt ggctataaag aacggttaga aacatcctat cgaacaccat
    32281 acggtatgat ccagattgct acttttacga aagaactgca tgttagtttg aaagaccgtc
    32341 ccacagcagg gaaggttcga atcgattatg atctgttcat gggaccggaa aaaatcggcg
    32401 aatattattt gaccttggat tttacagcgt aaatataaaa gaaaagtttg attttttgtg
    32461 tctgattttg tatgataagg agtagactgt gaaaggacgt gtcctgattg gaaattagtg
    32521 tatttgaagg tgcaaacaaa agcgaattat caatgatcga agtagcacac gcgatcttag
    32581 aacaacgtgg cgatgtcatg gatttttctg atttagctaa ccagatccaa aactatcttg
    32641 ggaaatcaga cagtgaaatt cgtgattcac tcgcacaatt ttatactgac ttgaatattg
    32701 acggcagctt tatctcatta ggggataatc gttggggctt gcgttcatgg tatgctatcg
    32761 attcgatcga tgaagaagta aaccatggaa tggacgaaga ggatgaagat actccacgtc
    32821 gccgtaaacg caagaaagtc aacgccttta tcaatgatga tgaagacgcg atcgattaca
    32881 atgacgatga cccagaagat acagatctga cagaagaaga tgacgatgat ctgtttgatg
    32941 acgatgatga tgaagatgaa gaaatcgctg cttataattc tgacttgcaa gaaatcggag
    33001 cagatgaccc gacagatgat gaagaagact tgccaggaaa tatcgaagaa gatttaagta
    33061 tcatagacga agacgatgat gatgaagatt atgaagagga agaattttct gactctgaaa
    33121 aagaataaac attattttct tacgagaggt tgtgacagag acgatcagcc tatcagaata
    33181 aggaggtgtg acacgatttt tgtcacacct tcttttttgt atataaaaat cccaagtaaa
    33241 tacataaaaa ataaaaaaat tgaaactttt agcgcacctt gtacgtcata ttaagtgagg
    33301 cagaaaggag tggagaatga tttttactgg atataagaaa aaacggcaga tcgaaaaata
    33361 ccgtcacctg ctgaatggac tcatcgaaac tgattatcaa aaaatgttcc gtatcacgat
    33421 gaattacgta catgataaag aagaggcctt agatgtgatg caagatagtt ttcataaagc
    33481 cctgaactat tttgaaaaag aaaaagagat cgagcatttt agtgcatggt tttatcggat
    33541 attgatccgc acggcattag atagttggcg cagacaaaaa aggacgcctg tggacttatt
    33601 tgaaattcaa gatatccccc aacagttacc tttagaaact tccgttgcag agctccatat
    33661 gattttagat aaaatagaca gtccggaaaa agagattctg atcctctact tttttgaagg
    33721 atttcgtttg aaagaaattg cgactatctt ggatatgaat gaaaacacag ttaaaacgaa
    33781 aatgtaccgc tcattgaatg ctttacgtca aatcctagaa tgaaggagag aagagtgcca
    33841 tgtattccca aaaagaaaat ttaaaaaaac taaagaaaac ttataaagaa caagcaattc
    33901 ccaaagggct taaagaggag atccgccagc gttttattat cgaagagcaa tcatttataa
    33961 aagcaaagcg gagaaagaga cgtctccaag ggttcggagt atctttggct atcgtaatga
    34021 ttacaacaag tagccttctt tttaatagtc aagttcgaag ctttgcagaa gatttaccca
    34081 tcttaggatc agtcattgaa ttgatcttag gcgaacgctt tactgatcga tctgaaaaaa
    34141 tcgatattca ggtaccgaag atcaatacac aaaatgagaa agaaaataaa acaattcatg
    34201 gattaaatca aaaatacttt cgcgaaggac aagcagattt tgaaaaagct gaaaaagaat
    34261 atggaaattt tgaaacggat cattaccaag ttttgggaga ttatcagaaa atcgtagacg
    34321 ataaccggtt tctcgtgatt gaaaggcaga tcacccaaac agcagcagat agccatgttg
    34381 aaaaaagata cgacacaatc gacaagaaaa acagtgtcca gctctcactt ccacttttat
    34441 tcaaagatga cacctatcta tctgttttga caaaagaagt caaacggcag atggccgaac
    34501 aagtcaagga agatccatct aagtactatt ggactgagca agatattcaa gaagggacga
    34561 tagaaaaacc aacacttgtt acgcctacac gaagttttta tttgaacaaa gagcatcaat
    34621 tagttttgac tttttctcaa tacgaaattg ctcctggtta tatgggtaca ccagaatttg
    34681 tcattcctaa gtccgtgaca aaaacgattt tggcatcgga agattatttg gatcattaga
    34741 cgagaagtag ttgcgactag cagtttcaat tttcaagtaa gtacaagtaa ataataagaa
    34801 gagaaaaaga gaaagaagag atatagtgaa aagaaattat ttagttatca gtattattgc
    34861 tttatccgtg cttttgtttt tcgaaattac ttcggtggcc tccttgatca ccgaagtaaa
    34921 aggacaaaca cactcttcgg agcattcgtc acaatctttc aaacaatcag aacaatcaac
    34981 cattaaaagc caatcaactg aaacagctaa cgtcaaagaa gccattcaag aatcagcgga
    35041 aaaggtacaa agtcagtcat cacaatcaac acaagacagt acaaaaacga gccgagatcc
    35101 ggctgtcaaa agcgtcgcaa tcagctttga tgatggacca ggagcgacca caacaccgca
    35161 gcttctgcga atcttaaaag aaaaaaacgt gcatgccact tttttcgtct taggagaaaa
    35221 tacggcgcag catcctgaaa tcgtgaagca gacagctgaa gcagggcacg agattggcaa
    35281 tcacacatac gatcatcaga atctggccac tctttctgcc cagtcgatga cggaggaagt
    35341 gacgaaagca gacactgaga tcaaaaaagt cactggcaaa acccccactt ttgttcgtcc
    35401 gccctatggt tctgtcacga atgtaggagc aacgatcatc cagcgaccga tcatcgaatg
    35461 gtctgttgat tcagaagatt ggaagacaag aaatcctgat ttgattttgc agaagatcca
    35521 agcaacagta tacgatgggg caattatttt attccatgat atctatcctg aaacgattcg
    35581 tgccgttcct caagtgatcg attatctgaa agagcaaggg tatcgcatca cgacagttgg
    35641 ggatttatta gggcatccaa cggctgtcga gaactattat ggcagaaacg atcatcggcc
    35701 agttcagtaa gtgagacaag tatctttaat aaataacaca ttttaataag tagttgaaca
    35761 cataactacc ttacttgcaa aggcttaaca atatctatac aggtacaaca aaaaactgtc
    35821 caccaatttt ctttggcgga cagttttcac ttaaaaataa agaagcaact attcgtaaat
    35881 tatgctaaaa tagcagtaga acgcgtcctg agggaatcga acccccgtct caagaaccgg
    35941 aatcttacgt gatatccact acactaagga cgcaagcact gggataattg taactaaaca
    36001 gataaaaaat tacaagggga ttggacaaaa aaatgaaatt tgagcaaaat ttttcacaaa
    36061 aacagcagca gacacagaaa ctagccatga cgcaacagtt gcaacagtcc atccaagttt
    36121 tacaatataa cagtgaggag ctgttggcat tcgttgagaa tcaagcaatg gaaaatccat
    36181 tggtggaagt tgttgagccg gaatggcagc cagattacat aaaagcttct tcatcttctt
    36241 atgaaggaga agagacaaat tacctaaatc agattcctga tacaaaaggg tctttatttg
    36301 attcattgat cgaacaagtc catttgaatt accgagatac ttttttgagg aaaatcgttc
    36361 tttatcttgt agaatacatc gatttgaacg gatttttgac gattagttta gaagaagccg
    36421 gaaaagagat tggagctacg cctatccaaa tgctcgatgc gttgactctg attcagcaat
    36481 tagaaccagc tggagtaggt gcacgtagtt tgcaagaatg tctcatgctt caaacagaac
    36541 gagatgatta tgcgccggaa ttagcttata tcgtgttgga agaatgtttt gaggaactgg
    36601 tggagcgaaa atggaaagaa atcgcacaac gtttttctgt tgatctacat gctgttcagc
    36661 agatattcga ctatttacaa acgttgagtc caagccctgg ccggattttc gaccgtagca
    36721 gcgaattgta cattattcct gatgtccggg tattagtgga cgacgataaa aatgtacaag
    36781 ttatctcaaa ccgaaaaaat cagccaaata tacgctttca agagagttac ttcaaacaaa
    36841 tgtcacaaca agcagataaa gaaactgaaa attatttaaa agaacggaaa caggaattcg
    36901 aatggttgaa gaaaacgatc ctacaacgtg gggatacaat tttgcgggtt gctcaagtca
    36961 ttgtttcccg gcaaaaagct ttctttatcg acaaggaacg tccaatcaag cctcttacgt
    37021 taaaggaagt tgcaactgag atagatgtgc atgaatccac ggtcagtcgt gcagtcaacg
    37081 ggaaatattt ggaaacagac ttcggtgtat ttgaattaaa aaaatttttc actacacgaa
    37141 ttcctacgaa tagtacagaa cagacagaag acctttcagc agatacagcg aagaaaaagc
    37201 tgcaggaatt agtcgatcaa gaagacaaaa acaagccgtt gtctgaccag aaattggttg
    37261 aattgctgaa aaaagatgag atagcgatct caagacgaac agtagcaaaa tatcgtgatt
    37321 tattaggaat cccgagttca tcaaaaagaa aacgttatga taactaatca gtcaaaagac
    37381 tagaaaaagt atttcacttt ttctagtctt tttagttgaa aaacgagagc aaaactgtta
    37441 gacttacagt cgtgaggttg agatttccct ttattgatgt gaaattcgaa tagttaattt
    37501 ctttttttat cgtatatggg tcgttttaag tctacattgg acgtaaagtg tccaactaag
    37561 gagatcgcta tgctggatga aataaaaatg attgaatctg ttgcaccaga tattatcgaa
    37621 gtgttgcaag aacgctataa aattttacga aatatctatt ggatgcagcc gattggacgt
    37681 cgaagtttat cggaaagtat ggggatcaca gaacgagtat tgcgaacaga aacagatctt
    37741 ttgaaaaatc tgaatttgat cgacacctca aaaagcggta tgacgctgac aactaagggc
    37801 gaagaagttt atcaaagcct tgaaaacttc atggatcagc ttttaggtac gcatcaaacc
    37861 gaacaacagt tggcagaata ttttggtaac caacgctgta tcgttgtttc aggaaacagc
    37921 gaagagcaga ccaaagtggc tgatgcattc ggcgaagcgc tgagcgaagc cctagaccga
    37981 ctacttccag aaggcgagaa tatcatcgct gtgatgggag gaactactat ggctgtggta
    38041 gccgaacaat tgtctaatct ggaaaataag aaacggcata acctgtttgt ccctgcaaga
    38101 ggcgggattg gtgaagcgat aactgtacag gcaaattctg tcagtgcaag aatggctgct
    38161 aaagcaaacg gtaaccaccg tgcactttat gttccggaac aattaagtct ggcaacttac
    38221 aattctttac tcaatgagcc atctatccaa gaagtcttaa acttgattag tgaagcgaat
    38281 tgtgttattc atagtattgg gcgtgcatta cacatggcag cacgtcgtaa gatgactgaa
    38341 aaagaattag tcatgctgaa acaagcaaat gctgttgcgg aatcttttgg ctactttttc
    38401 aatgaaaaag gtgaagtcgt ttataaggtc cctagaatcg gcatagaatt gcaagatttg
    38461 gaaaaagtcc cgatcatcat ggcaatcgcg ggcggaaaat caaaagccaa agcaattcga
    38521 gcttatatga aaaatgcacc aaaacaaacg tggctcatca ctgatgaagc tgctgcaaat
    38581 gagattttaa aaggggtaac cctttaaaat aaatattttt ttgattttca taaggaggaa
    38641 atcttaaatg acagttaaag taggtattaa tggttttgga cgtattggac gtctagcatt
    38701 ccgtcgtatc caagatgtag atggaatcga agtagtagca atcaacgact tgacagatgc
    38761 taaaatgttg gcacacttgt taaaatacga cacaactcaa ggacgtttca acggaacagt
    38821 tgaagttcat gaaggttcat tcaacgtaaa cggaaaagaa gttaaagtat tagctaaccg
    38881 caacccagaa gaattaccat ggggcgaact aggcgtagat atcgttctag aatgtactgg
    38941 tttcttcact tctaaatcag cagctgaaaa acatttaaca gctggtgcta aacgcgttgt
    39001 tatctctgct cctggcggaa acgatgttcc aacaatcgtt tataacacaa accacgaaac
    39061 attaactggt aaagaaacag ttatctcagg tgcttcatgt actactaact gtttagctcc
    39121 tatggctaaa acattgaacg acaaatttgg tgttgttgaa ggattaatga caactatcca
    39181 cgcttacaca ggtgaccaaa tgactctaga cggacctcat cctaaaggtg acttccgccg
    39241 tgcacgcgct gctgctgcaa acatcgttcc taactcaaca ggtgctgcta aagctatcgg
    39301 tttagtaatt cctgaattga acggtaaatt agacggagct gctcaacgtg ttcctgtacc
    39361 aacaggttca ttaacagaat tagtaacagt tcttgaaaaa gaagtaactg ttgacgaaat
    39421 caatgcagca atgaaagaag cttcaaacga atcttacgga tacaacacag acgaaatcgt
    39481 ttcttcagat atcgttggta tgacttacgg ttcattattt gatgctacac aaactaaagt
    39541 aatgacagtt ggcgacaaac aattagttaa aactgttgct tggtacgaca acgaaatgtc
    39601 atacactgca caattagtac gtactttaga atacttcgct aacttataag atagacaaac
    39661 gtacaagtgt aaattataga catgtacatt taaaagcggg gaagcaatcg cgcttcttcc
    39721 gctttttttg ataataagtt tattcaggag gaaataaaat ggctaagaaa acagtgaaag
    39781 atatcgattt aaaagacaaa aaagttcttg tccgtgttga cttcaacgtt cctttgaaag
    39841 acggcgtgat cactgacgac actcgtatca aagcagcatt gccaacaatc aactacgttc
    39901 tagaacaagg cggaaaagca atccttttct ctcaccttgg acgtgtaaaa acagaagaag
    39961 ataaagaagg aaaatcttta gctccagttg caaaacgtct aggcgaatta ttaggtaaag
    40021 aagtaacatt cgtacctgaa actcgcggag aacaattaga agaagcaatc cgcaacatga
    40081 atgacggcga cgttgtcgta ttcgaaaata cacgatttga agatattgac ggtaaaaaag
    40141 aaagcaaaaa tgatacagaa ctaggtaaat actgggcttc attaggcgat gtatttgtta
    40201 acgatgcttt cggtacagct caccgtgcac atgcttctaa cgtaggtatc gcttcaactg
    40261 gtatcccaac agttgctgga ttcttgatgg aaaaagaaat caagttcatc ggtgaagcag
    40321 tagaagaacc aaaacgacca atgattgcaa tccttggtgg cgcaaaagtt tctgataaaa
    40381 tcggcgtaat cgaaaacttg attcctaaag cagataaaat cttgatcggt ggcggaatga
    40441 cttacacatt ctacgaagca aaaggaatca agatcggtaa ctctctagta gaagctgata
    40501 aagtggaact agcaaaagaa ttaatcgaaa aagcaggcga caaacttgtc ttgccaatcg
    40561 acaatgtctg tgcaccagaa ttttcaaatg atgttgaaac acaagtaatc gaaggagaca
    40621 ttcctgatgg tttgatggct ttagacatcg gcccagcatc agtaaaatta tttgctgata
    40681 cattacaagg tgcaaaaaca gttgtttgga acggacctat gggtgtattt gaaatgagta
    40741 actttgctaa aggtacaatc ggtgtttgtg aagcaatcgc taacttagaa ggcgcaacaa
    40801 caatcatcgg tggcggagac tcagccgcag cagcagaaca attaggattt gctgacaaat
    40861 tcacacacat ctcaacaggt ggcggcgcaa gcttggaatt gcttgaaggt aaagaattac
    40921 caggactagc agcaatcaac gataaataaa cgaccaagtt tgctcaattc tgacgagtaa
    40981 tcgatcaaaa ggtgaaggga gttttcatga ctccctagcc atttttcaat aaattaagga
    41041 gtgtgttcct caatgcgtaa acctattatc gcaggtaact ggaaaatgaa caaaactgca
    41101 tcagaagcaa aagaatttgc tgaagcagta aaaaacaaaa tcccttcaaa tgacaaagtt
    41161 gattcagtca tcggatctcc tgcattattc ttacaagaat taatgacatc agctgaaggt
    41221 acagaactaa aaatctcagc acaaaactgc tactgggaaa actcaggtgc gttcactggt
    41281 gaaacttcac cagcagctct tgctgatcta ggtgttcatt atgtgatcat cggacattca
    41341 gaacgtcgtg aatatttcca tgaaacagac gaagacatca ataaaaaagc gaaagctatc
    41401 tttgcaaatg gtatgacacc aatcctatgc tgtggcgaat ctttagaaac ctatgaagca
    41461 ggcaaaactg ctgaatggat cgaaggacaa attacagctg gtttagctga tctttcagct
    41521 gaacaagtgt caaacatggt tattgcttat gaaccaatct gggcaatcgg tactggtaaa
    41581 tcagctgacg cacaaatcgc tgacgaaatt tgtggagttg tacgtcaaac agttgaaaaa
    41641 ctttatggaa aagaagtatc agaagcagtt cgtatccaat acggcggttc tgtaaaacca
    41701 gaaaatattg ctgaatacat ggcaaaagaa aatgttgacg gtgctttagt cggtggcgca
    41761 agcttgcaag cagattcatt cttgtcattg ttagacgctg taaaataatt gttgaaaatt
    41821 tgcgaaaaat gcaagaaatg tttgcactaa tgagcaaaat taactaaaat cgtatatgag
    41881 ggaaatccct taggaaaaaa caaactcaaa ggagagacaa aacatgtcaa ttattactga
    41941 tgtttacgct cgcgagatct tagactcacg tggtaaccca acaatcgaag tagaagtata
    42001 cacagaaagc ggagcttttg gccgcggaat ggttccttca ggtgcttcaa ctggtgaata
    42061 cgaagcagtt gaattacgtg acggagacaa agctcgttat ggcggtaaag gtgtaactaa
    42121 agctgtcgac aacgtaaaca acatcattgc tgaagcaatc attggctacg atgttcgtga
    42181 ccaaatggct atcgacaaag ctatgatcgc tttagacgga actcctaaca aaggtaaatt
    42241 aggtgcgaac gctattttag gtgtttctat cgctgttgca cgtgcagctg ctgactacct
    42301 agaagtacct ttataccact acctaggcgg attcaacaca aaagtattgc caacaccaat
    42361 gatgaacatc atcaacggcg gatcacatgc tgataactca atcgacttcc aagaattcat
    42421 gatcatgcct gttggcgctc caacattcaa agaagctcta cgtatgggtg ctgaagtatt
    42481 ccacgcatta gcttcaatct tgaaagctcg cggattagca acttctgttg gtgacgaagg
    42541 tggatttgct ccaaaccttg gttcaaacga agaaggtttt gaagtaatca tcgaagcaat
    42601 cgaaaaagct ggctatgtac ctggtaaaga cgttgttctt gctatggatg ctgcttcttc
    42661 agaattctac gacaaagaaa aaggtgttta cgtactagct gattcaggcg aaggcgaaaa
    42721 aacaactgac gaaatgatca aattctacga agaattagtt tctaaatatc caatcatctc
    42781 aatcgaagac ggattagacg aaaacgactg ggacggattc aagaaattaa ctgacgtatt
    42841 aggcgacaaa gttcaattag ttggtgacga cttgttcgta acaaacactc aaaaattatc
    42901 tgaaggtatc gaaaaaggaa ttgctaactc aatccttatc aaagtgaacc aaatcggtac
    42961 attaacagaa actttcgaag ctatcgaaat ggctaaagaa gctggctaca cagcagttgt
    43021 atctcaccgt tctggtgaaa cagaagattc aacaatctct gatatcgctg ttgcaacaaa
    43081 cgctggccaa atcaaaactg gttctctatc acgtactgac cgtattgcta aatacaacca
    43141 attactacgt atcgaagacc aacttggtga agttgcagaa tacaaaggtt tgaaatcttt
    43201 ctacaactta aaaaacaaat aattcttttc taatgaagat acatgatcga ctattgaaag
    43261 atagtcgagt atgatcactt agttaagtaa tcgaactctc tacttgctga aatatcagca
    43321 ggtagagagt tttttttgca actagcttct gtatatgatg tttacaaaga atggtacaag
    43381 aaaagatcat ccttgaaaaa ggaaagctaa caataactag catcatataa gagcttttaa
    43441 ggatagaaca gtagaatagg aatctatgag caagtcctct cgtataaata aaataggagg
    43501 gaagtagcag catatttttc ataaagtggc tctctttctt catttttact ttggttgctc
    43561 ctatttgaaa attcgagcac actcaaatac aataaaaaga aatggcaagg gagaggatta
    43621 gatgaaaaaa gaagaaaaaa tcgcgatttt acaagagatc atccgaatta aatcagtgaa
    43681 tggaaacgaa ggtgaagtag cagcctattt gaataagctt ttggcaaagc atgacataac
    43741 aggagagatt gtttcttaca gagatggtag agataatttg attgctcgtt accaaaaggg
    43801 ccaatcagga aaagtgttag gcctatctgg gcatatggat gtcgtggcgg ctggtgatga
    43861 atcgtcttgg acctatgcac cctttgcagc agaaatccat gggaaccgtt tgtacggacg
    43921 tggtgctaca gatatgaaga gtggcttggc agcaatggtg attgccatga tcgaactgaa
    43981 agagtcagga aaaccattta acggaacagt caaattatta gccactgtcg gagaagaagt
    44041 gggtgaacta ggtggggaac agctcacgaa agcaggctat gtagatgact tagatgcctt
    44101 gatcatcgga gagccaacta attacagttt gatgtatacg cacatgggat caatcaacta
    44161 tacagtcacc tcgcacggaa aagaagcgca tagctctatg ccagaccaag gttataatgc
    44221 aatcaatcac ctaaatgaat tcattacgaa agcaaatgct gaaatgaatc acctcgctga
    44281 aacaatcgag aatcctgtcc taggaaaaac aatccataat gttacattga tcagtggcgg
    44341 gaatcaagtc aacagcatcc caagccatgc gcagctccaa gggaacattc gatcgattcc
    44401 agaataccca aatgataaaa tcattgcatt gctgcaatca atcgtcaatg aattgaatca
    44461 agagacagac tatcatttgg aattgatgat cgattacaac aaaatacctg tcaaagctga
    44521 tccagattct cctttgattc attgcattca gcaacaattc agccagccat tacctttagt
    44581 tggagcggca gcaacaacgg atgcagccga atttacgaaa gctaaccatt ccttcgactt
    44641 tgtcgtcttt gggcctggtg tcgttacttt gcctcatcaa gtagatgagt atgttgaaat
    44701 cgataattat ttggatatga ttgaaaaata tcaaggaatt attttatcct acttagcgta
    44761 agattttttt aataaatgaa aaaacagagc cagattagca ttattttgct aatccagctc
    44821 tgtttattca gcctattttt ctttttgaat atatcaagcg ttgtggcaac atagtgatga
    44881 ctaatgaaac aacgacgata gacaacaaac gatcaaggta gtctgtacct gcctgtacca
    44941 agatcacact tgttgtctga cttaaaccta acccatgtaa tagctgaacg atcatactgg
    45001 atcctgagga ggtgatcccg ccaaataact gaacggtaat gatcgaagaa acgatcgttc
    45061 caggtaccga caaaatcaaa gcgagtaata acagtttcaa gctgttttta tctttgccga
    45121 gatatcttgg caaaatcaat ccagcaagca agcctgtgag aatttggatt ggcgaataat
    45181 acagagaaaa aatatctgta gtcactccac ttaaaatacc gctggacaat cctgtcagca
    45241 ttccaggtac aggcccaagc aatgcaccag caaaaatcgt tccgatagaa tcgagataga
    45301 ttggcaaacg aagccataaa gcaatcgttc ctccaatata attgatccca atacacaaag
    45361 ccatcactgt aagtacgcga gttgccattt tgtttttatt cattttgtta gcccatcttt
    45421 aacctttcca aatctttcat aatcgtttct tcttgtgcat ttaaaagaac ggttaaaaac
    45481 tttcggaaaa aaagacgagt atctactttt gtcataattt tactgttagc aggtttctgc
    45541 caaaattcat aacgatcaac caatgtttgt ccacgactta ttccttgagt ttccacagca
    45601 gtgaagcttt tgaaaccttc acagatggcc ccctcgataa aataagcaac agctaacgga
    45661 tcattgatga cacatcctaa aattctttct tgtttccagt ggaagtcaaa ataaaaacga
    45721 gtgatcgctt ttagatactc tccctcttca ggattcattt gacagcagta ttcgaggata
    45781 gtcggtgtca agacgatttc tcttgtcaca tctaacccaa ccatttctat cgtttgtttg
    45841 agattctcga atacataaga agctgcttca ggatcacacc agtaattgta ttcagcaact
    45901 ggcgaacagt taccatggct tttgtaagtt ccacccatcg agacaaaacg atccatatgt
    45961 ttcccaatat ttgggttgac tttcaaagca aaagcgatat tggtcaaagg acccaaagca
    46021 atgactgatg tatttgtctt ttctttgaaa taatcagcca aaaaatcaac ggcatggaga
    46081 ggttctgcct gttttctcaa gatcatcggg aaatttgttt cccctaaacc atccatacca
    46141 tgagtgtctt gtgcactgat gaaaggttgc ttcaaaggtt tttccattcc ttgataaaca
    46201 ggaatgtcta aacgatcaca gcgttccagg catttcagca cattctgcgt acctatatct
    46261 gcagggacat tcccacaaac tacggtaata gcaactactt cgatatcagg ggatttgaca
    46321 gctaatgata aagctaaagt gtcgtcgatc cctggatcgc aatcaataat tacttttcgc
    46381 attatttcca atctcctttc tttagagata cttagttttt tattctggga agtttccgaa
    46441 ccagtccaag acgagcgtct tgattataat gcacgaaatt agtatactgg cttttattgc
    46501 gaatgtaaat gctctcttac aagtaaatct attgaggaga actttaagta aatgatacaa
    46561 tgaaaaagaa atggaattta cgtgtgccga aaataaagaa tcctatggat cggtacattg
    46621 gataatagtt agattatagt ttggcagaaa gcggcgggtg tacaatggtt attttttatt
    46681 tcattcttat tctgataatt atagggatca gttaccattt tctttcttat ttttatgcaa
    46741 agaaattagt aagagcggga gtacagagag gatatgactg gtatgaagac actggtcatc
    46801 gtttgatgga ccctgctgca gtcacgtcag tagaaaaaaa acgacaaaaa ttagaagaag
    46861 agtgtgaaag cttttgggag cagggaacac ccaaaacaat caaaagttat gacgggttga
    46921 aactagctgg tcagatgttc atccatccaa ataagcagaa taaatgggtc atttgcgtac
    46981 atgattaccg aagcacaggg aaaagagaca tgtctcatat aggtaaaaga tatgcagaga
    47041 aaggattcaa cgttcttgta cctgatttac gtgcgcacgg agaaagtgaa ggagaaatca
    47101 tcggaatggg atggctagat cggctagatt taattgcctg gatccaactc atcttagatg
    47161 aacagccgaa tgcgtccatt attttacacg gcggatcaat gggagcttct acgatcatga
    47221 tggcaagtgg tgaaaaactt ccatcagcgg tcaaaggatt tattttagac agcggatatg
    47281 tttctgttta tgcagaattc cgatatatgt taagtaaaat cactgtcttt cctaaaaaaa
    47341 tggtcatgag atatgcgaat cattatgccc agaaatacgc cggttactcc ttaaagcaag
    47401 catcagctac gagacagctt ggcagcaacc atctgccttt attggtcatt catggtgaaa
    47461 gagatcattt tgtccctaca gaagcagcgt acacgattca aaatgctacg gcaggggata
    47521 aagcactgct gcttgttcca gaagcagaac acttggaagc atcgatgaaa gatccgaaaa
    47581 cctattggac agtgattttt tcatttattg aacaaagaat aacgatgaat taattttctc
    47641 ttcttttttg taagtaattc gaggataaat aattgtttat agatcaggag actgctaaaa
    47701 taaggtagga ataaaaaaat atcagttatt tgaggagtgc ggcatatgga aatcaaagaa
    47761 gaaaaaaatc gtcttgcttt attcaatgat gaacaacaag agatcggaga aatgacatgg
    47821 tcagatgcgg gcccagacat catgatcatt gaccatacat ttgtagatcc agcatatcgt
    47881 ggtcaaaagt tagctgaaaa gctagtgtat actggagtag aattagcaag acgagacggg
    47941 aaaaagatca ttcctttatg cccttatgca aaaaaagagt ttgaaaagaa accagaatat
    48001 caagacgtct tacgtcagtc ataagaataa cagctgaaag ttttgaactt tcagctgtta
    48061 ttccatttat atagagttgt cacggacttt tatctatgat aaagttaaag agacgatatc
    48121 ttttgggaag gaggctgcga tatgtataat ttaattttgg gaatcgtgat cgttttatcg
    48181 attgttatgg tcattgcgat tatgatgcag ccaagcaagc aaaatagtgc agcgagtgca
    48241 tttaccggcg gtgcggatca actattcggc aaacaaaaag ctcgcggctt tgaagctgtc
    48301 atgcagcgat caactgcagt tatgggagct atctggatga tcttattatt tgtcctagca
    48361 tatttatctt cgaagtagtt gaataagcct ctcgcaaaac ggcgggcggc tttttttatt
    48421 gcaaagagaa cgcttaagaa gaagttatga aagaaactat ggtaaaatga ggcgaaagag
    48481 gtgagacgag tgagacaatt accaaaaccc ctttatgcaa aacatggaaa gcgagcggtt
    48541 ttgctgcttc acgcttactc aggaagtcca aacgatgttc gaatgctcgc acgatttttg
    48601 gaaaaagcag attatacagt ttatgcgccg ctttttaaag gacacggaac aatggagcca
    48661 tatgatattt tgcaagaaaa agcagaaagc tggtgggcag acactaaaaa agccatccat
    48721 tttttacagt ctgagcagtt ttctgatatt gccgtattag gattatcaat gggcggcatc
    48781 tttgctgtac gagcactaga agaagaatca gtgattggcg gcggtttttt ctgttcgcct
    48841 ttgtctcctg taaaaacaaa cgtgcctgaa aactttgaaa aatacgtacg tcaagtgctg
    48901 aagacagcag gtaaatcaga gaaagaaatc aatgaaaaag cagttgctta ccgctcgtta
    48961 gctgaacaac aattaatgga tatacaagat caggcagcaa tcgtagaaag taggttgtca
    49021 gatatccaac agccgatatt tttagctcaa gctgggaaag atgaaatgat cgaccctaat
    49081 ggcgtatttg aaactgcacg caagttgagc cgtcagcgag tgacacttcg atggtatcct
    49141 gaaagtggtc atgtgattac agttggtaca gcaagacgcg aattagaaaa agacgtcttg
    49201 gaatttttag aaaaattacc ttggaatgag gaataaatat gacaaaagaa acgataaaag
    49261 agaaaattct ctttttcatg gaaagcagtc gcaaacgcag tttttctatg gaagaaatcg
    49321 ctgaaggttt agggttccaa aaaagcgatg attttaaatt actagtacaa acgattgctc
    49381 aaatggaaag agaacagagt atagttttca ataaaaaagg aaaagtgaaa cttcctttaa
    49441 aaccaatctt gatcgaagga acatttcgag caaatgaacg aggatttgga tttgtaacga
    49501 ttgatcccga agaagatgac atctatatcc caaaagaagc gactggtttt gcaatggatg
    49561 gagacaccgt agcaatcgat atcatgaagc gagcaaatac agatatggat cgcggagcag
    49621 aggggaaagt agtcgagatt cgagaccggg cgactactca gttagcaggg gaattcgttg
    49681 cttattcaga agaagaaata caagaaacgg atctttacgg cgttgttatc ccgaaagata
    49741 gaaagctcaa ccaatttaaa gtatatgcag cagcagaggg agttcgtcca gttgacggaa
    49801 gtattgtgct gatcgaactg acccattacc ctgaaaaagg gtatgcgact agtttagaag
    49861 ggataatcaa gcaagtgatt ggtcataaga atgaccctgg catggatatc ctctcaattg
    49921 tcgtggctaa tggtatccct acgaaatttc ctgatgatgt attgtctgaa gcagatgaag
    49981 ttccagatca aatcagcgaa aaggatataa ctggacgtag agatttaaga aatcagctca
    50041 ttgttacgat tgatggagaa gatgcaaaag atctagatga tgcagttacg gttaaaaaat
    50101 tagataacgg taattatttt cttggagtac acattgcaga tgtttcttac tatgtgacac
    50161 aaggcagtca attggacaga gaagcatatg aaagagggac aagtgtatat ttgactgatc
    50221 gagtgattcc gatgatcccg caacgtttat cgaatggtat ctgttcactg aatccccatg
    50281 ttccaaggct gaccatgagt tgcgaaatgg agattgatcc aaatggacaa gtagtctctc
    50341 atgatatttt cccaagtgtg atccaaacaa cagaacggat gacctacaca gctgtcaatc
    50401 aaatcttaga agatcaagac gaagaaacga tgcaacgcta tgctcagctc gtacctatgt
    50461 tccaagaaat gggtgaattg catcgaatcc ttgaaacgat gcgaatcaaa cgtggcgcga
    50521 tttcttttga ggatcgcgaa gcaaaaatcc ttgttgatgc tgaaggacac ccaaatgata
    50581 tccagctaag aagcagagga attggtgaac gcttgatcga atctttcatg cttgcggcca
    50641 atgaaacggt agctgaacat tataataaat taaatctgcc gtttatttac cgaatccatg
    50701 aacagccaaa agaagaaaaa atgcagcgct tctttgattt tgcttcagct ttgggcattc
    50761 tggtccgagg aacgaaagga acgatcacac caaaagattt acagcatgtg atcgaaggag
    50821 tagaagacaa accagaagct gcagttatca atactatgtt gctaagaagc atgcaacaag
    50881 cacgctattc agaagacaat tttgggcatt atggcttagc ggcagattac tacacacact
    50941 ttacatctcc tattcgacgc tatcccgatc taatcgtcca tcgtctgatc cgttcttaca
    51001 gtgaagataa aagtgaaagc aatcaaagtt cgtgggctac ggaacttcct gaaattgcag
    51061 atcatagttc taagatggaa cgtcgtgcag tagaaacaga acgtgaagta gattccatga
    51121 agaaagcaga attcatgatg gacaaagtgg gggaagaatt cgatgggatc atcagctctg
    51181 ttgtgaaatt cggcctgttt atcgaactgc cgaatacagt agagggattg atccatatca
    51241 atgaattgaa acaggactac ttccattttg ttgaaaatca tttagctctt gtaggtgaaa
    51301 gaactggtct gacttttaaa atcggacaga aagtgcgagt aaaagtagtc aaagctgatc
    51361 cagatgaacg agcagtagat tttgaacttg tttccgcaga agaggttaca gcgttggaac
    51421 ggccaaaaac acgaaatgga aaatcaggaa aacgcgaagg aaaacaagca ggaaaacgcc
    51481 cagaaagaaa tggcaagaag aacaaacgat atagcgatag taagcaaacg ttcggtaaga
    51541 aaaaagggaa gaagccattt tacaaaggtg ttaaaaagaa gaaaaagaaa aaataaaatg
    51601 agttggagga aacagctatg ccaaaaggcg agggaaaatt aattgcacaa aacaagaaag
    51661 ctcgccatga ttattcgatc atcgacacga tggaagcagg gatggtcttg caaggaaccg
    51721 agatcaagtc gatacgaaac agccggatca atctaaaaga tggatttatt cgcgtccgca
    51781 acggagaagc tttcttgcat aatgttcata tcagtcctta tgaacaagga aatattttta
    51841 atcatgatcc gttgcgcacg agaaagttat tattacacaa aaaacaaatc atccggcttg
    51901 aaaatgaatt gaaaaatact ggaatcactg ttgttccttt aaaagtctat attcgtaacg
    51961 gctatgccaa ggtattgatt ggtctggcga aagggaaaaa atcttatgat aaacgggaag
    52021 atttgaaacg aaaagatatc gatcgacaaa ttgatcgaac attaaaaaat ttctctagat
    52081 aatttcatta tcctttttcg catattcggt gcttttgctt ttacttaatg ataaaatttg
    52141 ttagcattta atctgataaa acatgattat cataggtttt ttcttagatc aggagcaccg
    52201 tgtataaaga aaaattcgct tcgtttttga ctgaaataaa aaagtgtcta attttcatga
    52261 aaatgttttg aattttctgt ttcatggtgg tatgataccg tgtggttaga aaccgattgg
    52321 aaaaaaccag atacgcacat gaattgattt tatgtgaatt tcggggaaaa gaggtgaaat
    52381 ggattggatg aacgctcgct aacgttccac attggaccaa tttggtttga tggtacagtt
    52441 tgtataatgg tgttactgac ctgtttgatt gttttcttct tagcttactt ttttacaaga
    52501 aatcttaagt tgaaaccaac aggcaaacaa aatgcgatag aatgggtcat tgattttagt
    52561 cgaggaatcg taacagataa tctgcctaga aaagaattga gcaatttcca tttgttggca
    52621 tttacattgt ttttatttgt ttttgttgca aacaacatcg gtttagtaac aaaaatcgtt
    52681 ttacctaacg aaacaacact ttggaaaagc ccgacagcag acccgtttgt tactttaact
    52741 ttggcattta taatgatctt acttactcac ttatttgggg tgaaaaagtt aggcttcaaa
    52801 ggatatttcg taaattcgtt cttgaaacca tatagtttta tgtttccgat gaaactgatt
    52861 gaagaattta caaacttatt aacacttgct ttacgtcttt atgggaatat ttatgcgggg
    52921 gaagttttgc taaccttgat tgcaaaaatg gtaataagct taggctggtt ctcattgccg
    52981 ttagcaattc cattagagat ggtctggatc gcattctcct tgtttatcgg aagcatccaa
    53041 gcatttgtct tcgttacttt atctatggtt tatatgagcc ataagattga agtggaagaa
    53101 taatattcgt tttaaaaaca aactactatt tatctaattt aggaggaaaa caattatgaa
    53161 ttatatcgca gcagcaatcg caatcatggg agcagcaatc ggagccggtt atggtaacgg
    53221 tcaagttatc tctaaaacaa tcgaatcaat ggctcgccaa cctgaaatgt ctggtcaatt
    53281 ccgtacaaca atgtttatcg gggtagctct agtcgaagct gttcctatct taggggtagt
    53341 tgtcgccttg attctagttt ttggtgttta attgaatcag tattaaggca gaaacaattt
    53401 ggtttgttac tgctttttta catgaaaact agaagtactt caaagaaagg aaggctcgcc
    53461 tatgctgaat caattggcaa ttgcagaagt tggcaatgcg atgttaggaa atgtcatcgt
    53521 tgtcagcggc tcatttttga tactactggc tctcttgaag cattttgctt ggggaccaat
    53581 cagcgatatt ttgaaaaaac gtgaagacaa gatcgccaat gatttggatt ctgcagaaca
    53641 atctcgcatc aactcagcga aaatggaaca agaacgcgaa caacaattgt tagcctctcg
    53701 ttctgatgca gctgatatca tcaaaaatgc gaaagaaagt ggagaattaa gccgccaaaa
    53761 tattttgaag gatgctcaag aagaagcagc tcgtctaaaa agtaaagccc aagctgatat
    53821 cactgtagaa cgtgattcag cgctgaactc tgtaaaagac gatgttgcag aactatctct
    53881 tcaaatcgcg gaaaaaatct tgaacaaaga attatctcca gaaatgcacg aatcattaat
    53941 caatcaatat atcgaaggtc taggttcttc aaatgaaact agataaatat acagtaggaa
    54001 gacgttacgg gaaagcttta tttgaactgg cgatcgattc aaataaagca gaagaaattt
    54061 atcaagatct cgtaagctta cgtcaaatct atgaacaagt acctggatta ggtaatatgt
    54121 taagtgatgt tcgtctagaa cctcatgaaa aacgagaaat catggacaag cttgttagtg
    54181 gctatgatgg aatcgttaaa aactttttag aagttgttta tgcgtataac cgaatggatg
    54241 atcttttgtt catgatcgaa gaatatgaac gtcgatatga tgaacatcaa ggtttgctgt
    54301 tgggaagtgt cacaacagca gttccacttt cagacgaaca acgaatacaa ttagaaaaga
    54361 atgttgcaaa aacaatgaac tatcaatcag tcgaattaaa acaaatcgtt gattcttcta
    54421 ttattggtgg cgcgattgtt gaagctaacc atcgagtaat cgacggaagc attcgcacgc
    54481 aattagaaaa tatgcgtaat caattaaata gatagtacaa agaggtaggt gaatcgaatg
    54541 gccatcaaag cagaagaaat tagtgccttg attaaagaac aaattgaaaa ttatcaaaac
    54601 gtgctttcag tcgaagaaat cggtaccgta acttatgtag gtgacgggat cgctcgtgca
    54661 catggtttag aaaacgcgat gagtggtgag ttgcttgaat tttcaaatgg ctcatacgga
    54721 atggcgcaaa acttagaatc aaatgatgta ggtatcatta tccttggcga tttcgagtcc
    54781 attcgtgaag gagataaagt aaaacgaaca ggaaagatca tggaagttcc agttggagag
    54841 gccttgattg gacgggtagt taatccgcta ggtcagccaa ttgacggact aggtgaaatc
    54901 gttactgaca aagctcgtcc agtagaagca atggcaccag gcgttatgca acgtaaatct
    54961 gttaacgaac caatgcaaac aggtctaaaa gcaatcgacg ctcttgtacc aatcggacgc
    55021 ggccaacgtg aattagttat cggtgaccgt aaaacaggga aaacttccat tgcgatcgat
    55081 acgatcatca accaaaaagg tcaagatatg atctgtatct atgtagcaat cggacaaaaa
    55141 gattctacag ttcgtacaca agttgaaaca ttgaaaaaat atggcgcaat ggattacaca
    55201 atcgttgtga atgccggtgc gtctcaacca gcgccgttgc tttatatcgc accatatgct
    55261 ggtactgcaa tgggtgaaga gttcatgtac aacgggaaac atgtcttgat catttttgat
    55321 gatctttcaa aacaagctgt tgcttatcgt gaactttcct tgctgcttcg ccgtccacca
    55381 ggtcgtgaag cttatccagg ggatgttttc tacttacact cacgcttatt ggaacgtgca
    55441 gcaaaattaa gtgatgattt aggtggcggt tcaatgactg ccttaccatt tgttgaaaca
    55501 caagctggag atatctctgc ttatatccca acaaacgtta tttcgatcac tgatggacaa
    55561 atcttcttag aaagcgactt gttctacgca ggtactcgtc cagcggttga tgccggacta
    55621 tctgtatctc gtgtaggggg ttctgcgcaa atcaaagcaa tgaaaaaagt tgctgggact
    55681 ttacgtctag acttagcaag ttaccgtgaa ttagaagcat ttacacaatt cggttctgac
    55741 ttggatgcag caacacaagc aaaactaaat cgtggacgcc gtacagtaga aatcttgaaa
    55801 caaaaattgc atgcaccgct agctgttgaa aaacaagtag tgattttgta tgctttgaca
    55861 catggattct tagatagtat ccctgtagac agtattttag acttcgaaca tgaattattc
    55921 gaatatctgg ataccaatca ttcagatatc tttgaaacaa tccgtacaac aaaagatctt
    55981 ccagaagaag aagcgttgaa cagtgcgatt caggaatata aagacatgtt cttagctaca
    56041 aaaggaaata attcctctac agaagataaa ttgaaatcca ttcaaaatgc atagtgaggt
    56101 gagatgaatg ggtgcttcat taaatgaaat caagacacgt atcgcttcaa cgaaaaaaac
    56161 gagccaaatc acgcgggcaa tgcagatggt gtctgcttct aagttgacaa aatctgaagc
    56221 ttcttcacag aagtttcagg tttatgcaac aaaagtaaga gaaatcgtta cgcatttgac
    56281 tgctactcag ttaaatgata ttgcctcaga aaatcctaga ggggatatca attacaacag
    56341 catgttgctc agccgtcctg ttaagaaaac gggttatatc gtcatcactg cagatggcgg
    56401 tttagtcggt ggatataaca gctcgatatt gaaacaaacg atgtctattt tagaagaaga
    56461 ccaccaatca cccgatgatt acgtgatgat cgcaattggc ggaactggtg cagacttctt
    56521 taaagcacgc ggtatcaatc tagcatatga gctgcgcaat ctatctgatc agccaagctt
    56581 tgatgaagtc cgcaaaatcg taaatatggc aacaactatg taccaaaacg aagtattcga
    56641 tgaattatac gtatgttata accatcatat caattcgcta actagtcagt ttcgtgtaga
    56701 aaaaatgctg ccaatctcag acttagatcc agaagaagca actactttcg aacaagaata
    56761 tatttttgaa ccttcaaaag aagaaatctt gtctcagtta ttgcctcagt atgctgaaag
    56821 tttgatctac ggtgcgatcg ttgacgccaa aacagcagaa catgctgctg gtatgacagc
    56881 aatgaaaaca gcaactgata atgctgctaa tatcattgat gagctgacgg tttcttacaa
    56941 ccgtgcaaga caaggggcta ttacccaaga aattaccgaa attgttgccg gagcttcggc
    57001 actagaatag tattgagatt ggaggaaaaa agatgagttc aggcaagatt gttcaagtaa
    57061 tcggtcccgt tgtcgacgtg gaattttctt tagaccaatc cttaccagat atcaacaacg
    57121 cattagttgt ttataaaaat gacgaaaata aatcaaaagt tgttcttgaa gcagcgttag
    57181 aattagggga cggagtgatc cggaccattg cgatggaatc aacagatggt ttacaaagag
    57241 gaatggaagt cattgataca ggcaaagcaa tctctgttcc tgtaggtaaa gaaacattag
    57301 gtcgcgtatt caacgtacta ggagatacga tcgatttaga aacacctttc ccagaagatg
    57361 cggaaagaag cgaaattcat aaaaaagcac cagcctttga cgaattaagt acaagtacag
    57421 aaattttgga aacagggatc aaagttatcg atttgcttgc cccatattta aaaggtggga
    57481 aagtcggact attcggtggt gccggtgttg gtaaaaccgt actgatccaa gaactgatcc
    57541 ataatatcgc ccaagaacat ggtggtattt ctgtatttac cggtgtaggt gaacgtactc
    57601 gtgaaggtaa tgacttgtat tatgaaatga aagattcagg agttatcgaa aaaacagcca
    57661 tggtgttcgg acaaatgaac gaaccaccag gtgcacgtat gcgtgttgct ttgactggtt
    57721 tgacgattgc ggaatatttc cgtgatgtag aaggtcaaga tgtactgttg tttatcgaca
    57781 acattttccg tttcactcaa gctggatctg aagtatcagc cttgttagga cggatgcctt
    57841 ctgcggttgg ttatcaacca acattggcaa cagaaatggg tcaattgcaa gaacgtatca
    57901 catctacgaa aaaaggttcg atcacatcga tccaagcaat ctatgtgcca gccgatgact
    57961 acactgaccc ggcgccggca acagcattcg cccatttgga tgcgacaacc aacttggaac
    58021 gtaaattaac agaacaaggg atttatccag cggtagatcc attggcttct tcttcaagtg
    58081 ctttagcacc tgaaatcgtg ggagaagaac attataaagt agcaacagaa gtccaacacg
    58141 tattgcaacg atatcgtgaa ttacaagata tcattgctat cttaggtatg gatgaattat
    58201 ccgatcaaga aaaagtgtta gtttctcgtg cccgcagagt ccaattcttc ttgtcacaaa
    58261 actttaacgt ggctgaacaa tttactggtc ttccaggttc atatgtccct gtagaagaaa
    58321 cagtgaaagg attccgcgaa atcttggaag gcaaatacga tgatcttcct gaagaagctt
    58381 tccgaagtgt tggccgaatc gaagacgtcg tagaaaaagc gaaaacacta ggctactaga
    58441 aaggggtgcc ccatggatca atatttaaca gtaaatgtgg taactcctgc cggtttggtc
    58501 tatgatcacc atgcagctat cgtcgttgca cgtacgacag ctggtgagct tgggattttg
    58561 cctaaacatg caccaatcat cgttccattg acgattgatg aagttcgtgt aaaacgaaca
    58621 gattcagata cccacgtcga ttggatcgct gttaatggcg gaatcatgga agttcgtgat
    58681 aatgttgttt caatcgttgc tgatagtgcg gaacgtgaac gtgatatcga tgtatctcgt
    58741 gcggaacgtg caaaacaacg cgcagaacgc caaatcgaag aagcaaaagc aaaacaagat
    58801 acgaacgaat tgaaacgggc aactgttgcg ttgcaccgag caatcaatcg tatcaaagta
    58861 tctaagcact cataaaatga gatgcggatg tttatccgtg tttaagaaca gtaaaaagag
    58921 gttatgacag aagtggtcag cttcaagaca taagaaggaa tttacgaaaa ttgcttctca
    58981 aatttttgtg aaatctcagc ttatttttcg aagaagctac ttctggaaca ccgtatatcg
    59041 gaggttgtga aatcagcgtt ttgtcctgag tattagagaa acaaatggaa aatagttcct
    59101 catattttgc cgttttttga gctaataccg agggtcagat gattgaacac cgtttatttg
    59161 gaataaggga ctgcgacaag actttt
//
LOCUS       DACDIG010000007        57556 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.007, whole genome shotgun sequence.
ACCESSION   DACDIG010000007 DACDIG010000000
VERSION     DACDIG010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.007"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            4..816
                     /locus_tag="IV247_000503"
     CDS             4..816
                     /locus_tag="IV247_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAP8776599.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="IV247_000504"
     CDS             813..1613
                     /locus_tag="IV247_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAP8776600.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="IV247_000505"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="IV247_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAP8776601.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="IV247_000506"
     CDS             3110..4819
                     /locus_tag="IV247_000506"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAP8776602.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5031..9383
                     /locus_tag="IV247_000507"
     CDS             5031..9383
                     /locus_tag="IV247_000507"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAP8776603.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9527..10000
                     /gene="rimP"
                     /locus_tag="IV247_000508"
     CDS             9527..10000
                     /gene="rimP"
                     /locus_tag="IV247_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAP8776604.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10024..11199
                     /gene="nusA"
                     /locus_tag="IV247_000509"
     CDS             10024..11199
                     /gene="nusA"
                     /locus_tag="IV247_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAP8776605.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNSKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11221..11514
                     /locus_tag="IV247_000510"
     CDS             11221..11514
                     /locus_tag="IV247_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAP8776606.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11511..11822
                     /locus_tag="IV247_000511"
     CDS             11511..11822
                     /locus_tag="IV247_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAP8776607.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11835..14141
                     /gene="infB"
                     /locus_tag="IV247_000512"
     CDS             11835..14141
                     /gene="infB"
                     /locus_tag="IV247_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAP8776608.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14168..14515
                     /gene="rbfA"
                     /locus_tag="IV247_000513"
     CDS             14168..14515
                     /gene="rbfA"
                     /locus_tag="IV247_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAP8776609.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14623..14844)
                     /locus_tag="IV247_000514"
     CDS             complement(14623..14844)
                     /locus_tag="IV247_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8776610.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            15292..17655
                     /locus_tag="IV247_000515"
     CDS             15292..17655
                     /locus_tag="IV247_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HAP8776611.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKLGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRNDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFHSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAKKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
     gene            18023..18946
                     /gene="truB"
                     /locus_tag="IV247_000516"
     CDS             18023..18946
                     /gene="truB"
                     /locus_tag="IV247_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAP8776612.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            18950..19891
                     /gene="ribF"
                     /locus_tag="IV247_000517"
     CDS             18950..19891
                     /gene="ribF"
                     /locus_tag="IV247_000517"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAP8776613.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            20243..21028
                     /locus_tag="IV247_000518"
     CDS             20243..21028
                     /locus_tag="IV247_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8776614.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            21041..21892
                     /locus_tag="IV247_000519"
     CDS             21041..21892
                     /locus_tag="IV247_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAP8776615.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(22016..22468)
                     /locus_tag="IV247_000520"
     CDS             complement(22016..22468)
                     /locus_tag="IV247_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8776616.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(22933..23301)
                     /locus_tag="IV247_000521"
     CDS             complement(22933..23301)
                     /locus_tag="IV247_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAP8776617.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            23451..23933
                     /locus_tag="IV247_000522"
     CDS             23451..23933
                     /locus_tag="IV247_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAP8776618.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            23970..24980
                     /locus_tag="IV247_000523"
     CDS             23970..24980
                     /locus_tag="IV247_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAP8776619.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(25095..25808)
                     /locus_tag="IV247_000524"
     CDS             complement(25095..25808)
                     /locus_tag="IV247_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8776620.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(25998..26588)
                     /locus_tag="IV247_000525"
     CDS             complement(25998..26588)
                     /locus_tag="IV247_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAP8776621.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            27072..27299
                     /locus_tag="IV247_000526"
     CDS             27072..27299
                     /locus_tag="IV247_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAP8776622.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            27315..28481
                     /locus_tag="IV247_000527"
     CDS             27315..28481
                     /locus_tag="IV247_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAP8776623.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            28715..29758
                     /gene="hrcA"
                     /locus_tag="IV247_000528"
     CDS             28715..29758
                     /gene="hrcA"
                     /locus_tag="IV247_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAP8776624.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            29852..30415
                     /gene="grpE"
                     /locus_tag="IV247_000529"
     CDS             29852..30415
                     /gene="grpE"
                     /locus_tag="IV247_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAP8776625.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            30466..32295
                     /gene="dnaK"
                     /locus_tag="IV247_000530"
     CDS             30466..32295
                     /gene="dnaK"
                     /locus_tag="IV247_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAP8776626.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            32446..33612
                     /gene="dnaJ"
                     /locus_tag="IV247_000531"
     CDS             32446..33612
                     /gene="dnaJ"
                     /locus_tag="IV247_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAP8776627.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(33694..34359)
                     /locus_tag="IV247_000532"
     CDS             complement(33694..34359)
                     /locus_tag="IV247_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid utilization regulator"
                     /protein_id="HAP8776628.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            34335..34508
                     /locus_tag="IV247_000533"
     CDS             34335..34508
                     /locus_tag="IV247_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP8776629.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(34736..34942)
                     /locus_tag="IV247_000534"
     CDS             complement(34736..34942)
                     /locus_tag="IV247_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8776630.1"
                     /translation="MICKASSGDEMAVKEILKFYDGYISKLCLRPFYHSESGKIIMQV
                     DEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(34968..35387)
                     /locus_tag="IV247_000535"
     CDS             complement(34968..35387)
                     /locus_tag="IV247_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009269612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP8776631.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            35891..36244
                     /locus_tag="IV247_000536"
     CDS             35891..36244
                     /locus_tag="IV247_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8776632.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            complement(36304..36471)
                     /locus_tag="IV247_000537"
     CDS             complement(36304..36471)
                     /locus_tag="IV247_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8776633.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(36590..38509)
                     /gene="tet(M)"
                     /locus_tag="IV247_000538"
     CDS             complement(36590..38509)
                     /gene="tet(M)"
                     /locus_tag="IV247_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAP8776634.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            complement(38525..38641)
                     /locus_tag="IV247_000539"
     CDS             complement(38525..38641)
                     /locus_tag="IV247_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAP8776635.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            complement(38886..38963)
                     /locus_tag="IV247_000540"
                     /pseudo
     CDS             complement(38886..38963)
                     /locus_tag="IV247_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646598.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(39117..39752)
                     /locus_tag="IV247_000541"
     CDS             complement(39117..39752)
                     /locus_tag="IV247_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8776636.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            40264..41406
                     /locus_tag="IV247_000542"
     CDS             40264..41406
                     /locus_tag="IV247_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP8776637.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRYPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CDGVIRRFDLAINDMCGLLDIPVLSEKYRNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRGTKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            41399..41605
                     /locus_tag="IV247_000543"
     CDS             41399..41605
                     /locus_tag="IV247_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAP8776638.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            41684..42877
                     /locus_tag="IV247_000544"
     CDS             41684..42877
                     /locus_tag="IV247_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8776639.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDRKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVLENDTKKKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQQVMKKRPKAEPFVIDGRGNFLFVNHKGKPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(42884..43042)
                     /locus_tag="IV247_000545"
                     /pseudo
     CDS             complement(42884..43042)
                     /locus_tag="IV247_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008788611.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding domain-containing protein"
     gene            complement(43048..43785)
                     /locus_tag="IV247_000546"
     CDS             complement(43048..43785)
                     /locus_tag="IV247_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP8776640.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(43787..45289)
                     /locus_tag="IV247_000547"
     CDS             complement(43787..45289)
                     /locus_tag="IV247_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8776641.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            45548..47542
                     /gene="uvrB"
                     /locus_tag="IV247_000548"
     CDS             45548..47542
                     /gene="uvrB"
                     /locus_tag="IV247_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAP8776642.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKEELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            47552..50368
                     /gene="uvrA"
                     /locus_tag="IV247_000549"
     CDS             47552..50368
                     /gene="uvrA"
                     /locus_tag="IV247_000549"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAP8776643.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            50631..51896
                     /locus_tag="IV247_000550"
     CDS             50631..51896
                     /locus_tag="IV247_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAP8776644.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            51900..52631
                     /locus_tag="IV247_000551"
     CDS             51900..52631
                     /locus_tag="IV247_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAP8776645.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            52766..53650
                     /gene="rapZ"
                     /locus_tag="IV247_000552"
     CDS             52766..53650
                     /gene="rapZ"
                     /locus_tag="IV247_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAP8776646.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            53647..54645
                     /locus_tag="IV247_000553"
     CDS             53647..54645
                     /locus_tag="IV247_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAP8776647.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            54679..55614
                     /gene="whiA"
                     /locus_tag="IV247_000554"
     CDS             54679..55614
                     /gene="whiA"
                     /locus_tag="IV247_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAP8776648.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            56157..56807
                     /locus_tag="IV247_000555"
     CDS             56157..56807
                     /locus_tag="IV247_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8776649.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            56994..>57556
                     /locus_tag="IV247_000556"
     CDS             56994..>57556
                     /locus_tag="IV247_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HAP8776650.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
BASE COUNT        19160 a        10055 c        12049 g        16292 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3721 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccagaacg cgatttcact gtgctttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4501 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgtgtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccagctagga cgaaatattc
     5701 cagccgatga accaacgaca ccaatgatca gtatcgttga agaagaacga agagtcacac
     5761 ttgaggggta cgtattcgac aaagaagtca gagaactacg ttcaaaacgc aagatcttga
     5821 ctttgaaaat cacagattat acttcatcat ttatcgtcaa aaaattctcg aacggcgaaa
     5881 aagatgaaca agtatttgat gcgatcagtg tcggtagctg gttaaaggta cgaggaagta
     5941 ttcaagaaga tacgtttgtc cgtgatttgg tcatgaatgc gcaagatatc atagaagtca
     6001 aacatacacc tcgcaaagat tatgctcctg aaggggaaaa acgagtagag cttcatgttc
     6061 atagcaatat gagtacgatg gatgcaacaa acagcatctc tgatcttgtg gcacaagcag
     6121 gaaaatgggg acaccgagct atcgccatta cagaccatgg tggtgcgcaa gcctttccgg
     6181 aagcacatag tgctggaaaa aaagcaggtg tgaaaatctt atacggcgtg gaagcgaatg
     6241 tcgtagatga tggtgtccca attgcttata atgatgcaca tgaagcattg agtgaagcca
     6301 catacgtcgt atttgacgtg gaaacgacag gattgtctgc agtatatgac acgatcatcg
     6361 agctagccgc agtcaaaatg tataaaggaa atgtgatcga aagctttgat gaatttatcg
     6421 atccaggaca tccattgtca cgaacaacgg tagatctgac tggtatcacg gatgaaatgg
     6481 ttcgagggtc aaagtcggaa gaagaagtat tgcggatgtt ccttgaattt tcaaaagata
     6541 caattttggt agcccacaac gcggcttttg atatggggtt cttgaatact agctacgcaa
     6601 ggtatggtat tcctgaagcg gccaatccag tcattgatac gttggaatta gctagatatc
     6661 tgtatcctca attcaaacgt ttcggattag gtgtgttatc taaaaagttt ggtgttagct
     6721 tggaacagca tcaccgagca atctatgatg ctgaagcaac tggtcactta gcatggatct
     6781 ttgtgaaaga ggcaatggac aatcataaca tgctttatca tgatcagcta aatgagcata
     6841 tcggagaagg agattcttac aaaagggctc gacctttcca tgtaacgatt ttagcaaaaa
     6901 atcaggcagg actgaaagat ttgttcaaac tgatttcaat gtcgaatgtc gaatacttcg
     6961 aacgcgtccc acgaatacca cgctcccaat tgaaaaagat gcgtgaaaat ctattgatcg
     7021 gctcagcttg tgataaggga gaaatattcg aagccatgat gcaaaaaggg gtagaagagg
     7081 caagaaaccg agcaaaattc tatgattata ttgaagttat gccaaaagcg gtctatgcgc
     7141 ctttgatcga gcaagaattg gtaaaaaatg aacatgattt ggaagaaatt atccaaaatt
     7201 tggtagaaat cggaaaaagt ttggataaaa tcgttgttgc aacaggtaat gtacattatc
     7261 tgaacgaaga agatgccatt taccgaaaaa ttttgatcaa ttctatgggc ggagcgaatc
     7321 cgttgaatcg tcatagtttg ccggatgtcc atttccgaac aaccgatgag atgctaactg
     7381 catttcactt tttaggggaa gaaacagcga aagagatcgt agtagaaaat acgaataaaa
     7441 tcgcagatat ctgcgaagaa gtgattccag tcaaagatga actttataca ccgaagattc
     7501 caggttcaga agatgaaata tcagaattga gttacacaaa agcaaaacaa atgtacggag
     7561 atccgttacc tgagatcatt caaaaacgat tagaaaaaga gttgaattct attaatggta
     7621 atggcttctc ggttatttat ctgatcgccc aaaaactagt gcataaaagt aatgaagatg
     7681 gctatctagt aggttcacgt ggctctgttg gctctagctt tgttgcgacg atgacaggaa
     7741 tcactgaagt taatccatta gcccctcatt attattgtcc agagtgccaa tattccgagt
     7801 tttttgaaga tggtacgtat ggttctggat ttgacatgcc agaaaaacaa tgtccaaaat
     7861 gtggtgcacg cttaaacaaa gacggtcatg atattccatt tgagacattt ttaggattcc
     7921 atggagacaa agtgcccgat attgatttga acttctcagg tgattatcaa gcagaagccc
     7981 ataactatac gaaagtattg tttggcgaag actatgttta ccgtgccgga acgattggta
     8041 cagtcgccga caaaacagca tatggctatg taaaaggata cgaaagggac aataatctgc
     8101 agtttcgaag tgcagaagtc gacagattag caaaaggcgc tactggagtc aaacggacaa
     8161 ccggccagca tccagggggg atcatcgtta ttcccgatta tatggatgtc tatgatttta
     8221 cgccgatcca atatccagca gacgatcaaa attcagaatg gaaaacgact cattttgatt
     8281 tccactcgat tcacgataat gtattgaaat tagatattct agggcatgat gacccaactg
     8341 ttattcggat gcttcaagat ttatcaggga tcgacccaca aacgatccct acggatgacc
     8401 cggaagtgat gcggatattt gccggtcctg aagtacttgg agtaagtcag gaacaaattt
     8461 attccaaaac agggacatta ggtattccag aattcggcac tcgttttgtt cgagggatgt
     8521 tagaagaaac gcatcctaca acttttgcag aactactgca aatttccgga ctatctcatg
     8581 gtacagacgt atggttagga aatgcggaag aattgattcg acgaggagat gcgacactgg
     8641 cggaagtaat cggctgtcgt gatgatatta tggtttattt gatccatgct ggattagata
     8701 gcgggatggc atttaaaatc atggaaactg ttcgtaaagg acaatggaac aagatccctg
     8761 atgaattgcg agaaacctat ctatcggcga tgaaagaaaa taatgtgccc gactggtata
     8821 tcgattcttg ttcaaaaatc aaatatatgt ttccgaaagc ccatgctgca gcttacgtat
     8881 tgatggctct tcgtgtcgct tatttcaagg tttatttccc tattttgtat tactgtgcat
     8941 acttttctgt acgtgcagac gactttgatc tagtctccat gtgcaaagga aaagatgctg
     9001 tgaaacaagc aatgaaagaa atcacggaca agggattaga tgcttccgta aaagaaaaga
     9061 atcaactgac agtgctggag ttagccaatg aaatgctgga acgaggattc aaatttggaa
     9121 tgattgattt gtataaatcg gatgccgtta actttgttat tgaaggtgat acgttgatcg
     9181 caccatttag agctgtccca agtttgggaa ccaacgtggc gaaacaaatc gttgaagcaa
     9241 gaaaagacgg tccattttta tccaaagaag atcttgccac ccgcggtaaa gtttcaaaaa
     9301 cattgatcga atatatgaac gacaatggcg tattgaaaga tctaccagat gaaaatcaat
     9361 tatcgttatt cgatatgcta taagcatctg ttttcctgtc tgcttgtaat tttaagcaga
     9421 gtatggtata gttcttatag gtaatgatac taactgtgag tgagcggatt tttcgctcac
     9481 tctttttaat ggaattacgc acttaatttt taaggaggcg aaacttttga gtagcgtcgt
     9541 tgaaacagtc acagaaatgg tgacaccgat tttagatgaa caaaattttg aacttgtcga
     9601 agtcgaattt gtaaaagaag gaaaaagctg gttcttacga gtatttattg acaaagaagg
     9661 cggtatcgat atcgaagaat gcgcctttgt cagtgagaaa ctgagtgaaa aacttgatgc
     9721 agcagatcca gatccgattc ctcaagctta tttcctggag gtatcctccc cgggagcaga
     9781 gcggccattg aaaaaagaaa gcgactatga aaaggcactt ggtgattatg tccatgtctc
     9841 cttataccaa ccagtagatg gcgaaaaaca atatgaagga tttcttcaat catttgatgc
     9901 tgaacagctt acattgaaaa tacgcataaa aacacgagaa aaagaattga cttttgaccg
     9961 aaaaaatatt gcaaaagctc gcttagctat ccaattttaa tcacggagag gaatcagaaa
    10021 aaaatgagta aagaaatgtt aaatgcgtta gacgctttag aagctgaaaa agggatctca
    10081 aaagaaatcg tgatcgatgc attagaagca gcattggttt ctgcatacaa acgtcactat
    10141 ggccaagcac aaaacgtcga agtggaattt gatcagaaaa aaggaaagat ccatgtctat
    10201 gcggtaaaag aagtaacgga agaagtgatg gattcacagt tggaagtctc attgaaagat
    10261 gcacttttaa tcaatccagc atatgagatc ggtgatacga tccgttttga agtaacacca
    10321 aaagattttg gacggatcgc tgcgcaaacg gctaaacaag ttattttaca gcgtgtacgt
    10381 gaagcagaaa gaacgattat ttataacgaa ttcagtgctt atgaaaaaga catcatgcaa
    10441 ggaatcgtcg aacgccagga taaacgttat atttatgtca atcttggtaa aattgaggca
    10501 gttctttcta aacaagatca aatgccaaat gaattttatc aaccacatga tcgaatcaaa
    10561 gtttatgttt cccgagtaga aaatacgtct aaaggtccgc aagtatttgt tagccgcagc
    10621 catccagacc tattgcgtcg cttatttgaa caagaagtac cagaagtcta tgatggaatc
    10681 gttgaaattg tcagcatcgc ccgtgaagct ggcgatcgtt caaaagtggc agttcgctca
    10741 aatgatccaa atatcgatgc agtcggtaca tgtgtcgggc caaagggaca acgagttcaa
    10801 gcaatcgtca atgaattaaa aggcgaaaac atggatatcg tcgaatggaa tgaagatcca
    10861 gctgtattca tcgcaaatgc attgaatcct gcacaagtca tggatgtgat cttcgatgag
    10921 acaaattcaa aggcctgcac agttgtcgtt ccagactacc aattatcgct agcaatcggt
    10981 aaacgcggac aaaatgcacg tttagctgct aaattgacta accataaaat cgatatcaaa
    11041 tcagaatctg acatgacgga attttatgaa aatcaagaac aacaaaaaga aacagaagaa
    11101 ctgcatgatg aagcaatcgt tcagtcagat ttaacagacg atgaatatga aacaattgca
    11161 ttcaacaatg aaacggtcga agaaaaacca gaagcttaac tttagtaagg aggcaaagag
    11221 atgaaacaaa ggaaaattcc tttacgcaaa tccgtggttt caggtgaaat gaaacctaaa
    11281 aaagagatgg tccgaatcac ccgttcaaaa gaaggggaag tatcgatcga tccaactgga
    11341 aaaatgcctg gacgaggagc gtatgtctca cttgagccgg aagaagtaca acaagcttgg
    11401 gataaaaaaa ttctcgatcg agtactcgaa acaacattaa cagatgagtt ttatcaagaa
    11461 cttcttgact atgtcactca ccaaaaagca cgtaaagagt tatttggaaa atgaatacag
    11521 ttaatcggca aaaagccatg aatctcatcg gtctagcgat gcgcgcggga aaactaatca
    11581 cgggtgaaga actgaccatt gctgaaatcc gcaaacaaaa agcaaaattg gtgtttgtgg
    11641 caaccgatgc aagtgagaat acaagaaaaa agataaaaga taagagttca tattatgaag
    11701 ttccttgctt tgagctattt tctgaagcag aaatcacgca gatgatcgga aaacctcgca
    11761 aggtattcgg aataatggat gccggatttg cgaagaagac caaggagcta attgaaggtt
    11821 aggaaggtga ttgcatgggc aacaaaagaa tctatgaatt agcaaaagaa ctcaaaaaat
    11881 ctagtaaaga cgtcgtggaa aaagcacaac aacttggcat cgatgtgaaa aaccatatgg
    11941 gtgcgattac tagcagtgat gagaagaaat tgcaacaagc gttcaaaaac ccaagcacga
    12001 aacaacaagc tgcacaacaa gcaagcagaa aaccagccaa tcaacagccg aacgatcaac
    12061 gtaaaaaaga aacaacgaac aatcaacaaa aaaataaaaa taaccgcagt tatcaagatc
    12121 gcggtcaagg gagcggtcaa gtgaatcaag gaaaaaaacc aacaacaaac caaaataata
    12181 cgggaaatac cagcagccaa aatcgtcaag gaacgagcca aggcggcaat caaaaccgta
    12241 ctaacaataa caacaaacca aacaataaca atcgcggcaa atttaataat aataaccgta
    12301 atcgttataa taaaaaaggt aaaaaaggca aacagcaaca atcaactaaa ccagctgtac
    12361 caccacgcaa attccgtgaa ttgccagatg tattagagta tactgaaggt atgaacgtag
    12421 cagatattgc gaaaaaaatt catcgtgagc cagcagaaat cattaaaaaa ttatttatgc
    12481 ttggtgtcat ggtcaatcaa aaccaagcac tggataaaga tacgattgaa ctgttagcga
    12541 cggactatgg catggaacca caagaaaaag tccaagttga cattgctgat atcgataaat
    12601 tcttcgaggc agaagaagtc gatccagata aattagtctc acgtccgcca gtagtaacga
    12661 ttatgggaca cgtagaccac gggaaaacaa ccttattgga tacattgcgc cattcacgtg
    12721 taacaagcgg cgaagccggc ggaatcacac agcatatcgg tgcctaccag atcgatattg
    12781 atggtaaacc aatcactttc ttggatacac caggacatgc ggcctttaca agtatgcgtg
    12841 cacgtggagc aagtatcaca gatatcacga tcttagttgt tgctgcagat gatggcgtca
    12901 tgccacaaac agttgaagca atcaaccatg ccaaagctgc aggcgttcca atcatcgtcg
    12961 ctgtaaataa aattgataaa ccaggtgcaa atcctcagca tgttatgcaa gaattaagtg
    13021 agtatgaatt aattcctgaa gcatggggtg gagaaacgat ttttgttgag atctcagcga
    13081 aattcggcca aaacatcgaa gaattgctgg aaatgatcct gttagtcgca gaagtagaag
    13141 atttaaaagc agatccaaca caacgcgcaa tcggaacagt gatcgaagca cgtcttgata
    13201 aaggtaaagg tcctgtgtca accttacttg tccaagaagg aacacttcac gttggtgacc
    13261 caatcgttgt cggtaatact tacggacgtg tccgtgtaat gacaaacgat cttggccgtc
    13321 gcgataaaga agcaggacct gcaacgccag tagaaatcac tggcttgaat gatgtacctc
    13381 aagcaggtga ccgtttcgta gtcttcgaag acgaaaagac agcacgtgca gcgggtgaag
    13441 aacgcgcaaa acgtgcatta ttggaaaacc gtgctgccac aagccgtgtg actttggaca
    13501 acttatttga aagcctgaaa gaaggcgaat tgaaagaagt taacgtaatc atcaaagcag
    13561 atgtacaagg ttcagcagaa gcactggctg catcattgaa gaagattgat gtggaaggtg
    13621 tccgtgtaaa aatcgtccat tcagctgtcg gagcaatcaa tgaaagtgat gtaaccttag
    13681 cagcagccag caacgctatt atcatcggtt tcaacgttcg tcctacaccg caggcaaaag
    13741 tgcaagctga tactgaagaa gtcgatattc gtcttcaccg tatcatctat aaagccatcg
    13801 aagaaatcga aactgcgatg aaagggatgc tagaccctga atttgaagaa aaaatcaccg
    13861 gtcaaatgat cgttcgtgaa accttcaaag tttctaaagt tggtacgatt gcaggtgcct
    13921 atgtaacaga aggatatatt cgtagagata gcggtgtccg tgtaatccgt gacggaatcg
    13981 taatctatga aggccaactt gcaagtctga aacgattcaa agatgacgtg aaagaagtca
    14041 aaatgggtta tgaatgtggt gcgatgattg aaaaattcaa tgacatcaaa gtagatgacg
    14101 taattgaagg atttatcatg gaagaagtca aaaatgacta aaaactgata aaaggaggcc
    14161 aatcattatg gctaactacc gtgatcgccg agtcggtcaa gaaatcttaa aagaagtcaa
    14221 tgatattttg cgcaaaaagg tccgtgaccc tcgtgtggag aacgtaacga tcacagatgt
    14281 gaaagttacg ggggacttac agcaagcaac gatctattac agcattttat ctgacttagc
    14341 atcagataag aaaaaagcac aagatggatt gaataaagca tcaggattga ttcgtcgaga
    14401 gttagggcac aatctaagta tttataagac acctgaattg ttctttgaac ttgatgaatc
    14461 agttgtctat gggaaccata tcgatgaatt gcttcgcgac ttgaacaaag actaatcaaa
    14521 aaatcccgca aatcctgtga aatcaggggt tgcgggattt ttttgttttc attaagacgt
    14581 attacctaag agttccatga ctttagtctg gtagcgtgtc agctaaccaa aaaagagaaa
    14641 aagaaaagta aaaaattcga ctttcttttt tctatagtat ataaattttt gcatatagat
    14701 aaagatcaag ataccgataa cggaagctag attgtaatct attttagtga cagtcaggta
    14761 tagccaagta actagaagat cagtggaacc tgcaccataa aagtttgagt ttgtcggtcc
    14821 gttgacagtt aaaagaaaaa tcacgttgaa gttattgata tttccgatta cgccgcttcg
    14881 atttggtcac gatccaaatt ttggataatc cctgtagaaa ttagcatagt agctgggata
    14941 ccaattcaca tattgacaaa gataacagag acttttacat aaaacggatc aatcaggaaa
    15001 ggaattgctc gatccagccc cagttttcca acaatgtgtt aactggccta gcgtcattca
    15061 gcaaattatg catgatgagg agcgaaatga actgcggaac agccattgtg ataacgacaa
    15121 gttttttgga tcaatgtaaa aaacgtcagc gaaaggaaaa atttcgtggt tttttatacc
    15181 tgttcatttg gctagatata aaatccaaac aaatgaacag aagtgcttat taggtttcaa
    15241 ttgggagtat ctgcgctaga ataagagtaa taatgaaaag gagtgttatg tatggattat
    15301 tctagtaaag aatactttga taaaatgacg gcttggtggc gtgctgctaa ttatctttct
    15361 gttgggcaac tttatcttaa agataacccc ttactgagac gtacactgaa accagaagat
    15421 gtcaaaaaac atccaatcgg tcattgggga acgattcctg gccaaaattt tatttatgtc
    15481 catttgaacc gagtcatcaa taaatatgat ttgaacatgt tttatatcga agggccagga
    15541 cacggcggac aagtcatggt atcgaatgct tatttagatg gaagttacac agagatctat
    15601 ccagaagtaa cggaagatga gacaggaatg caaaaattat tcaagcgatt ttcatttcct
    15661 ggaggcattg cttctcatgc agcaccagaa acaccaggtt ctatccatga aggcggagag
    15721 cttggatact ctctttcaca tgcagtcgga gctgtattag acaatcctga agtaatctct
    15781 gctgtcgtga ttggtgatgg tgaagcagaa accggtcctt tagctggaag ctggttctca
    15841 aatgtcttta tcaatccagt gatagatgga gctgttttac cgatccttca tttaaatggt
    15901 gcaaaaatag ccaacccaac gatcttggca cgtaaatcag atggagaatt agcaaattac
    15961 tttaacggac ttggctggga gcctttcttt attgaaggaa atgatccaga aaaattaaat
    16021 cctgttatgg ctgaaaaaat ggatcaagca atcgagaaaa tcaaatccat ccaaaaagaa
    16081 gctcgcctga agacagcaac agatgtcgtg atgccaaaat ggccagtatt gatcgtacgc
    16141 acaccaaaag gatggaccgg tccaaaagaa tgggacgggg aaccaattga aggaaccttc
    16201 cgagcacacc aagtaccgat tcctgtagat caagaacata tggatcatgc agatgcttta
    16261 ctccgttggc tgaaatccta tgaaccagaa aaattatttg atgcacaagg acgtatttta
    16321 gaagaaatca gagaaattgc gcctactggt gaccaaagaa tggcaaaaaa tcctatcacc
    16381 aatggtggaa tcgatccaag accgcttatt atgccagatt ggaaaaaata tactttgcaa
    16441 tttgaaaaac taggctccgt taaagcagaa gatatgaccg aacttggtaa attcgttcgc
    16501 gagatcattg agaaaaatcc agaaaatttc cgtatatttg gaccagatga aacaaaatca
    16561 aaccgattga atcaagtttt caaaacgaca aatcgccaat ggatggaaaa aatcgaacca
    16621 gaaaatgatg aatggctttc gccatctggt cgcgtcatcg acagtcaatt atcggaacac
    16681 caagatgaag ggttcttaga agggtacgtt ctgactggac ggcatggttt ttttgcaagt
    16741 tacgaatcat tcttgcgagt ggttgactct atgttgactc agcacttcaa atggatacgg
    16801 aaatcacatg acctttcttg gcgaaatgat tatccttcgc taaatctcat tgcaagctca
    16861 acagtcttcc agcaagatca caatggatat agccaccaag atccaggaat attgacacat
    16921 ttggcagaaa agaaggcgga gttcatccgt gaatatctgc ctgcggatgc aaatacattg
    16981 ctcgcagtca tggataaagc attccactcg tcagaaaaaa tcaatttgat catctcttcc
    17041 aaacatccgc gtgctcaatt ttactcagca gaagaagcag ccgtcttagt gaatgaaggc
    17101 ttgaaaatca ttgattgggc ttcgacagca aaagaggagg aacctgaatt ggtcattgct
    17161 gctgcaggaa cggaatctaa cttagaagca ttagcagctg taactttgct gctggaagaa
    17221 ttcccaaaac taaaaattcg gttcatcaat gtcgtagatt tattgaaatt acgtcatcca
    17281 agtcaagatc cgcgtgggtt gagcgatgaa gaatttgatc aatattttac aaaagacaaa
    17341 ccgatcttat ttgctttcca cggttatgaa acattggtcc gcacgatttt ctttgatcgt
    17401 cataaccatc atttaatgat tcatggctac aaagaaaatg gagatatcac gacaccattt
    17461 gacatgcgtg tagtaaatga acttgatcgt tatcatttgg caaaagacgc agctttaaaa
    17521 atcaaaggaa gtcaagcaga agattttgcg aaaaaaatgg atcagaaatt acaagaacat
    17581 caaaactata ttcgtgaaaa tggtattgat ctgccagaag tattggattg gaaatggaaa
    17641 aatcttgacc aataaaatga taaaatcgat cccgcaaaat agaatttttt acattgatta
    17701 agaaactgaa aaaataaata aaaaaaacgg aaaaaagact ggaagaatgg ccagtctttt
    17761 ttttcgtttt tgggaagtga aaaccaaact atttacctgt aagccaccca taaatccaat
    17821 caaatggtgc aaagaaaaaa tcttccattc tattccctcc tttaaaaaag cataacatga
    17881 tttctattta cgtgagcatg aattttgtta cgtttttatt gccgaaaaat aaaaaccgtt
    17941 cattatgatg tcaaattcgt cagtaattga tgcaatgtgt tataataatt cagttaaatc
    18001 aaaaatgaaa tggagaaaag acatggacgg gttattacct ttatggaaag aacgcggaat
    18061 gacaagtcat gattgcgtat tcaaattaag aaaaatctta catacgaaaa aaatcggaca
    18121 tgggggtacg cttgacccag acgtagacgg tgtgttgccc atttgtattg gaaaagccac
    18181 aaaagttatc gaatatttga cagattctgg gaaaatctat gtaggcgaga taactctagg
    18241 tttcagtaca acgacggaag atcgatccgg agaacgggta gaaacaaaac cagtagatat
    18301 gtctttatct gaaaaaaaaa tagatgaagt gatggcttca tttattggtg agatcaccca
    18361 gataccacca atgtactcag cggtaaaagt caacggtaag cggttatatg aatatgcccg
    18421 caataatgaa acagtggagc gtcctgttag aaaggctcag atttaccgtt ttatccggac
    18481 aagtgaattg acaagagacg aagtgacagg aaccatttct tggcgcttcg aagtagaatg
    18541 cgggaaagga acctatgtcc gcacattagc tgtagataca ggcaagaaac ttggttaccc
    18601 tgcgcacatg tctgatctga cccgaacagc aagtgccggt atgtcccaga aggatgctct
    18661 gacattagaa gaagtagctc aagctgtgaa aaatgggaca atcgatgatt gtttgctgcc
    18721 aatagaaaaa ggagtggaac ggttcaaacg agtagatatt actgatgaag tatggcagaa
    18781 agtaaaaaac ggtatgagac ttgattatac tgtatttgcg ctcccagaaa tgccgtcaga
    18841 agaaattgct ttgttttata aaggaaaagt tgtcagtatt tatcagccaa acccgaaaga
    18901 aaaaaacaaa ttaaaaccta gtaaagtatt gagaaatgag gaataacgaa tgaatatcat
    18961 caagatcaga catccttacc aatcagaaca aattccagaa gacgatgtcg ttttagtgct
    19021 gggatttttc gacggcgtgc atatcggaca ccagaaagtc ataaagacag gtatggaaat
    19081 tgcacaaaaa gaaggattga aattagcatt gatgactttc aatcagcatc catctattgt
    19141 ctttaaaaag atcgatccta gcagcgtgaa atatttgact agccttaagc aaaaagaaga
    19201 aaaaatggcc gctttgggta tagattattt atatgaagtt gattttactt cttcttttgc
    19261 tcatttagca ccgcaggaat ttgttgatca atacatcgta gggttacatg caaaatatgc
    19321 tgtttccggt tttgattata cgtatggacc aaaagacatt gcagatgtcg ctcattttcc
    19381 tgaatatgcc aaaggaagat tcaaaatcgt cacagtttct aaagaagaag aacaaggtga
    19441 aaaaatcagc tctacccgta ttcgtggatt actagacagc ggacaagtaa aagaagcagc
    19501 aaaactatta ggttgcacat atgaggtaga aggggtagtt gtccatggcg atgcacgagg
    19561 cagactacta ggattcccta cagcaaatgt caaagtaaag agcacggttc atttgcctaa
    19621 agaaggtgtt tatgtgtcgg aaataaaaat tggggaaaca tggtatccag ccatgggttc
    19681 aattggacat aatgatacat tcggtgaagg acgggagctg actgtagagt tgtatatttt
    19741 ggactttaat caagatatct atggtgaaca tgtcgcggta cgctggaatc aatttttaag
    19801 agatcaagtg aaattttcta gtgtagagga attgattgaa caactcaagg aggatgaaca
    19861 agcaacagct gaatattttt ctcagcaata aaacattatg cataaaatct cttgtttatg
    19921 tataactagc aattacaata aaatgggtga taaataagcc caggaaaggg gctgtgacaa
    19981 aagtcttgtc acagcctttt tttccaaata aatggttttt cagaagtagc ttcttcggaa
    20041 ataagccgaa atttcttgaa gctgaccact tctgtcacaa cctctttctt tatagaaata
    20101 aaaggacact agcaattcat gaaaacgatt gctagagcgt atcatttgct ttagactata
    20161 tatcttggtg tacactcact ttaaggcgga aataaaagtt atccgtaata acaggtttag
    20221 gtatgaaaaa ggagtgggta ctatgtacaa ggaattagaa aacaaagtcg cagtagtaac
    20281 tggtggctca aaggggattg gaacagcgat tgctcaacga ttcgggaaag aaaaaatgaa
    20341 tgtagttgtc aactataact cggataaaga aggagcagaa aaagcagtca aagcaatcga
    20401 agaggcaggc ggaaaagcag tctctgtaca agcagatgtc agttctgaag aaggaataca
    20461 gcgtttactt gatacggctt tagaaaattt tgggacactg gacgtttggg ttaataatgc
    20521 tgggatggaa aatcagcatc caacccatga acttagcctt gaagattggg aaagagtgtt
    20581 aaaagtcaat ttgactggcg tttttatcgg aacgaaagcg gctttaaatt actttacaga
    20641 gaataataaa aaaggaaata tcatcaatat gtcttctgtt catgaacaga tcccatggcc
    20701 aacgtttgct cattacgctg cttcaaaagg cggggtaaaa ttatttaccg aaacggtagc
    20761 aatggagtat gcaccgaagg gaatcagaat caatagtata ggccctggtg ctatccgtac
    20821 acctattaat gcggcaaaat ttgatgatcc tgaacaaaaa gaactcttgg aaagtatgat
    20881 cccactagga cgcgtaggtg aaccaaaaga aatagcggcg acagctgcat ggttggcttc
    20941 agacgaatcc agttatgtta caggtattac actatttgca gatggtggga tgactttata
    21001 tccttctttc caaggtggaa aaggataagg gtgaataaaa atgggtttat tgattgcatt
    21061 gatcccggca gttggctggg ggatccagcc actgattttg aaaaagattg gtggcagacc
    21121 aacaaatgag atattaggaa ctggtattgg agcggtgctt gtcggcttac tcgttcaatt
    21181 attcgcttct ccagctggta tttcagtgac tacatttttg attagtttac tttctggtgc
    21241 attttgggtt attggacaaa caggacagta tacatctttc aacttgattg gtgtttctaa
    21301 aacgatgcca atttcgactg cattacagtt agtcggaacc tcgttgattg gtgtcttcgc
    21361 ttttggagaa tggtctggaa gtatggggaa aatcattgga gcagcagcaa tcattcttct
    21421 tgtatttggt tctgctttaa cggcaatcag tgatgatcct ggaaaaaaag gcggtatcac
    21481 aaaaggaatt agtctattag catcaacaag tattggttat tgggtataca gtgcattgcc
    21541 taaactagtt gatgcaagcg gactttccat tttctttccg caaatgatcg gtgtcttttt
    21601 aggggcagtg atttatgtga ttttccgtaa accaaccgca ttttctgaag acaagagttg
    21661 gaaagcaacg atcgttggag ccatctttag tgtgtcagca ttggcttata ttttttcagc
    21721 aaatgaaaat ggggttgcta ctgcatatat cattacacag ctaaacgtag ttatctctac
    21781 attaggcggt atgtacatcc tgcatgaaaa taagacacca aaagaaaaaa aattaacgat
    21841 tatcggatta ttgctgatcg tagtaggaag catggtcacg gtctttattt gaggataaag
    21901 atggtaaaat ttcatttatt aaaaaaatat ttgtttgaac caaacagtaa gtacttataa
    21961 aaaaatagcg ctagaatttc caacatctct ggaatttcta gcgcttttta attgtttaag
    22021 atacaaggga aatatctgtc tggatcaata atttttcatt gatccattga ctaaagttta
    22081 cgacataatt gaagttacca ccgattagta tctccgcatg attgtcttta taaagatcgt
    22141 attgtaaaag acggtctttt ttatttgcgt aaacatcgtt atggtactgg actaattgtt
    22201 ctcggaattg attgattgct gtaaaatcac gatattgaag gttgaaacgc atcgtgattg
    22261 gtgcatttag catgaatcgc tgcatttctt ttgctgcttc ctctgcttga tctccgtata
    22321 ggaactcaaa aagattacgt tcagaaaatt tccattgccc gccaaccttt gttcctttta
    22381 gttttccttc atgaagataa tttctgatag aacgtgtagt aacacctaat tgttcggcta
    22441 attcatttac tgagtaaaaa tctttcaatg ttcaccctcc tatgtgaatg gttctactga
    22501 cttagttgat atttgcttaa tcacgaaatg tagaaagaac gattttaatt aaggtcattg
    22561 taaaaatcaa gtcagaaaat tctaactttt atttctctaa agaaattcgt tctctcctaa
    22621 agtgatatta tatttaaata tacctgaaaa taatgggcag tattatgaaa gatcactgat
    22681 attttctcgt attttttcct tattcaaaag ttttgcagga aaattgctga aaaaataatt
    22741 ttactaagta gaaaattcaa agccattcaa tcagtgattt ttttcagcac aagaaagact
    22801 ccttataaaa atagcagata ctttgtcaat taagtgttta accagggatg ataaaaagga
    22861 taggattgaa caaaacctgt ataaggtgat gaatagtttc ctcgccagta aaaggtttat
    22921 tcgtataaag ctttatcgcc aaaatttatc atgacctttt ttatcttcaa tattaaatga
    22981 aatcaattgt tcaatcaatt cccagttgat tacacttttc caaggaactt tgatgacgtt
    23041 atccgtatat acgtagcctg cttcttggat atttttttca agggcacgaa tcgctgcttt
    23101 ttccggtgcg atagaaaaat gatttttagc aaaactaaat gcaataataa aggtaccatg
    23161 gtcagtaaac ataggttggt tccattttac agttgtgtct agctggggat acgtttctgt
    23221 tatcttagca aaaaggtctt tgatcttttg ttgatgttct ttttctttga tttgattgat
    23281 gatcggctga aatttttcca ttctaaaacg cctccattaa gtggtttgat ttattttacc
    23341 agaaaaacaa tagttatgta taaaaaacga acaaaacaca agtaatgttg taaccgctat
    23401 atttatatga taagatgtct caaataataa aatagaaaag agacataaaa atgattgact
    23461 tggcaaagaa attcttacac gaaaatatat attttgagtt gtatcatcat cgagccattt
    23521 atacaaatga agatgctttg gtaataaaga aagaacaagg atttacaggt acagagacca
    23581 aaagcttatt cttaaaagat aaaaaagata ataactacgt atatctgact tttactacga
    23641 aacaaacgga tttcaaagca ttagggaaga tcgtcggtaa aaggctttct attgtttcat
    23701 cagaacgcat ggaagaacaa acgggacaaa agcagggcgc tgtttctccg tttggttatg
    23761 aaaaacagat tccgattatt attgatgaag agctcttatc aaatgaaaaa cttgtttttg
    23821 caccaggtag accagatcaa acgatggtca tccaaacggt tgacttaaac aaaatcatcc
    23881 agctacttga gatgccgata tttttgatgc cggcaaacga aaaaaacaat taaccaagta
    23941 aaatagtgca tacttggagg tgaagcaaca tgataagtta tccattatta gaaagaaatg
    24001 caacgattgg ggttactgca ccatcctcag gcgtatcaga ggaacaccat tcgttactcg
    24061 aatcagcttt aaagagaatg gaagaaaaat ctttccaagt agttactgga aagacgcttt
    24121 ggacgcaaga taaggaaaaa tcaggtccag caagaaaacg agcagatgaa ttcaatcaaa
    24181 tgatgcaaga tgaaaatatc caaatgatct ttccgacttg gggaggagaa ttattgattg
    24241 agattctcga ttttgtcgat tttgatgctc tacaaccaaa atggatagta ggttattcag
    24301 atattagtac gttgctactt gctgtcacat taaaaacagg gatagccact gcacatggaa
    24361 ctaaccttat tgatttaaga ggagagtact tggatgaaac gacagcaatg tgggaaagtg
    24421 tactgaaaac aagagaagga gaatcgattc tacagcaatc ttctgaaaag tatcaaaaag
    24481 tgtggcccca tgagcattca tcatcttctg ttttccatct cactgaacca acaaattggc
    24541 aatcagtcag cggagatgca gtaaggatga agggacgatt attaggtggt tgtattgata
    24601 cgatcaggca tttaatcggt actccttatg gagatgtcca atcttttaaa agaaagtata
    24661 atgatggaaa gccgatcttg tggtttctag aaaactgtga gctatcgtca gttgatttaa
    24721 gaagatcact tgttcagatg aaactggcag gctggtttga tggcagctcg gggatcatgt
    24781 tcggtagaag tgcggcagag gatttgttag attataaaat gaaggatgta tacgaagatc
    24841 tgtcaaagga attaaaacta ccgatcttgt atgatattga ttgtggtcat gtaccgccac
    24901 aactgacatt gattaatggt gcctatgcag aagtagcggt agaagataga aaagcgactg
    24961 ttttgcaaac gtttatttaa aaaatttttt cctgccttgg agtctatcaa agaccaataa
    25021 atctaaatag aaaagaaagt gaaagaagct gacaaaatgc tgtcagcttc tttttgtata
    25081 acatattaga aagtctaatt atctataact gtcacatcga ttaactgttt cacgtcattg
    25141 ggatttcctg taggaatagc tactacagat gcattatcaa tgatttcttg agttgtttct
    25201 ctaatgaacg ctggataaga tgtttgatct gcaggcattt gccaatgtga aggaactgga
    25261 taactagtga ttgtcccgtc atggttactt gaatacacta aaactccctg gtaactgtat
    25321 ttcccagtga gcataaccac atctttcgga tagactgcac tccctgcatc ataaggggcg
    25381 ataggtgttc ctgcaggata atgaataaca tgtaattcga aatcacccga tgtatctcta
    25441 aaagtattac ctctaatagc tagccaaaca cgtgcgtact caatttgtag atcagaatat
    25501 cctgctaacg gatctattga ctgagaagac tgtgaggagg ttgtgtctat tgttgaagaa
    25561 gaaatatttg tctcaactgt actactagat gtagaagaag tattgggctc tgagacttta
    25621 ctgcttgttg aagatgtatt cgtttcagta gaagctgatt tctgagtact tgatttttca
    25681 gctgaactgg tttgtctgat tgaacttttg tctgtggtcg cagatggatc actattttct
    25741 ttactaggag acgtacatcc tacaaggaat aaagagacca acaataaaat aaaacctaac
    25801 tttttcatat aaaatacctc attatttttt taataccagt atagcataat cctgaatttt
    25861 tgaatccgta aaaccttgtc tatgaatata aatagagtgt atcttctttg ataaatgtca
    25921 gaaaaagtgt gacaaataat catcagtaaa attacttgtc acactattga taaggagtat
    25981 ttaattgaag agaatgatta ggagaaagga tctccgtact ttttatagta atcatagaat
    26041 ccaagtttgt atagacgaat cgttttttct cttatttctt ctgtttcaaa atggactgct
    26101 tgaagaatca agtctgtgaa ttctatagga gctgttccat tcgcagtaat caaattacga
    26161 tctttcacag cttgttgttc tataaaatct tctttattct gataattttc cgattctaac
    26221 caaggaaatt gcgcatttcc ggtgtgtttg taaccttgca ataatccgct ctgagctaaa
    26281 taatcaactg ccccacaaat agcagctact ggtacgtctg tttttaacaa tttatcgatt
    26341 atttgatata aatgatgatt ttttatgtcc catgaattgc caccgatcat aataaaaagg
    26401 tcaacttttt caggtatttg ttcgattaag taatcgattt ttgtttttaa tcctccaatg
    26461 gaagtgacct ctgattggat agaagcagtt ttgttttgcc atttctcatt ttggtttagt
    26521 gtgctagtaa tataagcacc ttcccaatcg gcatattcct ctaatagaaa cgtaatcgca
    26581 gttttcattt tcaagctctc ctttactatc tataacaagg acaacagttt taacctacag
    26641 acattcatta aaattgggta gcgacagcga aaacaacaat tcttaagttt tagacagagt
    26701 atacagatag tcaacttaat taaatacgaa ctaaataaaa atgacaggta atcatttttg
    26761 tttaccagca ttataacatt ttgcatacat ggatgacaaa agaaccaatt cccacttgct
    26821 tgagtacaat attaaaagaa actggaaggc aaaaagtaat tatagaaacg aaaaataaat
    26881 catatcgggc actcagacat gaaaacaacc ctagagatat ataactaggt aaccagttta
    26941 atgaaagaaa aaatcatcca agaagtcgat tcttggattt tttagtggat ttgttcgcca
    27001 aatacgttta gtatgcgtat atgagaggtt gtaagcaaag cgttgtggtc tgagcactta
    27061 aggaggaatc tgtgaaaata gcttttcata tttttgcaga ttttgactta atgccgaagg
    27121 tcagctttgc gattaccgtt tatcgagagg ctgtaaaagc accgtgtagc tccgatgaca
    27181 ataagcaagc atttttagag ctaggtttcc ttgattctaa atatgcggct tatttcaatg
    27241 agttggtgct tgaacgcgat tttaagaagg tcgaaaacag gcacgacttt tcgacgtaag
    27301 gagaaagaat ttcaatgata gaaacaataa atcccgcaga aaacaaggtt tctgcgtaca
    27361 ttcatatccc attttgcgag catatctgct attactgtga ttttaataaa gtttttttag
    27421 aagggcagcc agtagatgag tatgtggaaa tgctgctgaa tgaaatgaaa ataatgctag
    27481 aaagtcatcc gacagcgaat ttagaaacac tttacgtagg gggcgggaca ccaacttctt
    27541 tatctgctaa acaacttgat cgactgttat caggtgctag agagatcctg ccttttcaaa
    27601 atggtaatga gtttactgta gaagcgaatc caggtgattt gacccgtgaa aaacttcaag
    27661 taatgaaaaa ctatggcgtc aatcgtttat ccatgggagt gcagacattt gataatcgct
    27721 tattgaaaaa aattggacgt aaacacaccg cagaagatgt gtatcaaact atgcaatttt
    27781 tagaagcaga aaattttacc aatgtaagca tcgatttgat ttatgcatta ccgggacaaa
    27841 cactagaagg atatctggat actttggaaa aagctttggc tttggatctg cctcactatt
    27901 ctttgtactc attgatcctc gaaaacaaaa caatgttcat gaactgggta cgacaaggaa
    27961 gactcgaact gcctgatcag gaaacggaaa ccagaatgtt tgaagagacg attgaagcaa
    28021 tggaaaagca tggacgtcac caatatgaaa taagtaactt tgggctctcc ggacatgaat
    28081 ctcaacataa cctcatgtac tggaacaatg accactattt tgggtttgga gctggtgcta
    28141 gcggttatct tggagatatt cgttaccgca acaaaggacc aatccaacac tatttacgcc
    28201 cattacgagc aaatgaacgc ccggtattgg aagaagaaaa attgacacga aaaaatcaaa
    28261 tagaagaaga aatgttctta ggactaagaa aaaaaacagg gattactaaa aaacatttct
    28321 atgatcgcta tcaaaaaaca attgaatcgg tgtacggtga tgtgctaagg gaattagagc
    28381 aagaagggtt acttgttaat gaagaagacc gtgtttattt gacaccaaga gggacctttt
    28441 tggggaacaa tgtgtttgag cgctttttgt ttgaaaaata gtttaaagaa tatttgtttt
    28501 ttatctgaat ttaaataaaa aaagttcaaa aatacaataa aggactattg aaacgttgat
    28561 ataactggat ttacatcaat tatttagaat tagcactctt gtgtttagag tgctaaaaaa
    28621 tggagtcttt ctcttgacag gagaagggct tcttgctata tttataagtg gattagcact
    28681 tagaatatat gagtgctaat gagaggtgag gaagatgtta acaaagcgac aaaacgacat
    28741 tttacgtctg atcatccaac actatacgag tagtggcgtg cccgttggtt ccaaaacatt
    28801 gatgtctgag ggagtcaagg ccagctcagc aacgattcgc aatgatatga aagcattaga
    28861 agatgaagga ttgctgttga agactcactc ttcttctggg cggattccat ctgctttagg
    28921 ctatcgttat tacgtggatc atttgttaaa acctgctcgc gtagcagaag atgatttgct
    28981 gctgattcgt cagtctctag gcagagaatt tcatgaaatc aatgagatta ttaagcaatc
    29041 tgctgagata ttatctgagc tgacaagcta tacgactttt tcattgggac ctgaaataaa
    29101 agaccgccga ctgactggct ttcgtatcgt acccttgaat aatcgacaga ttattgctat
    29161 cgtcgtaaca gatatgggga atgtagaaag ccaagtgttc actcttcccg aatatatggg
    29221 aagtcaggaa ctagaaaaaa tggtacagat tgtaaacgac cgtctagtcg gagatcctct
    29281 gatgacggtg taccataaac ttcgtacgga gattccgatg attcttcata aatatttcca
    29341 aacgacagaa gggatgtctc agttatttga caccgttctt ggtcatgttt ttgaagataa
    29401 ggtttatgtc agtggacaaa tgaatctctt ggattatgag ccccgtcaag atcttgatca
    29461 gttcaaatcg atgttttctt ttatgaaaga ctcagacgga cttacacaaa tgatcactcc
    29521 gatggatagc aatattcata tcaagattgg tgctgaatta ggcaatgatt tattgcagaa
    29581 tatgagtatg attcaagcaa gttacgagat agatggtcat ggaagaggta caatagcctt
    29641 gttagggcct actagtatgc cttattctcg gatgttcggt ctagtcgatg tttatcgaag
    29701 agaactagca gctaaacttg ctgattacta ccgttcgctt gacttgtcaa actcgtaatg
    29761 tgaataacga agctatttgc agctgatcgt tctctctttc ttagtaagag caatcaaaca
    29821 gctgactgaa taatgaaagg aagactgtat cgtgaaagaa aatcaagaag aactagataa
    29881 agaaatgaca gatgctcagc cagaaccgga aatcgatgtc gaagctgcag aagattcggg
    29941 tatttcagaa gctgaagcgg aagaattcga aacagcgaaa ttaaaagctg agttagaaga
    30001 aatggaagat aaatacttgc gggctcgtgc tgaaatcgca aatatggcaa atcgaggaaa
    30061 aaatgagcgt gagcaattac aaaaataccg ttcacaagat ttagccaaaa aattacttcc
    30121 atcaattgac aacctggaac gtgcattagc cacagaagtg tcagatgatc aaggggcagg
    30181 tttgaaaaaa ggcgtggaaa tggtcttgga aagtttgcgt aacgcactgg aagaagaagg
    30241 aattgaaaaa atccctgcta aaggagaagc ctttgatcct aatcttcatc aagcagtaca
    30301 aactgttcca gcgactgaag atacaccagc cgatacgatt gtagaagtac tacaagaagg
    30361 ctacaaatta catgatcgcg tgttaagacc aaccatggtc attgttgcac aataattgtg
    30421 cggatataaa acttacactt tcaattaaag aaggagacgt taattatgag taaaattatc
    30481 ggtattgact taggtacgac taactctgca gtagcagtat tggaaggaaa cgaagcaaaa
    30541 atcatcgcta acccagaagg aaaccgtaca acaccttctg ttgtatcatt caaaaatgga
    30601 gaaatccaag taggggaagt tgcaaaacgt caagctgtga caaacccaaa tacgatttca
    30661 tcaatcaaac gccatatcgg tgaagcaggt tacaaagtag atgtagaagg taaatcatat
    30721 acaccacaag aaatctcagc aatgatcctt caatatatca aaggattcgc agaagactat
    30781 ttaggtgaaa aagtcgaaaa agcagttatc actgtgccag cttactttaa tgatgcacaa
    30841 cgtcaagcaa caaaagatgc tggtaagatc gctggtctag aagtagaacg tatcgtcaat
    30901 gaaccaactg cagctgctct tgcctatggt ttagataaaa cagatcgcga cgaaaaaatc
    30961 ttagtattcg accttggtgg cggtactttt gacgtatcta tccttgaact aggagacggt
    31021 gtctttgatg tattgtcaac agctggcgac aaccatctag gtggggacga ctttgataac
    31081 aaaatcatcg actacatggt agcagaattc aaaaaagaaa acagcattga cttgtctcaa
    31141 gacaaaatgg ctttacaacg cttgaaagac gctgctgaaa aagcgaaaaa agatttatct
    31201 ggcgtatcaa gcacacaaat cagcttgcca tttatcacag caggagaatc tggaccattg
    31261 catttggaaa tgacattgac acgtgcaaaa tttgatgaat taacagctga cttagtagaa
    31321 cgtacaaaag taccagtacg tcaagcgcta aaagatgcag gtttatcaca atcagaaatc
    31381 gacgaagtta ttttagtcgg tggatctact cgtatccctg cagttgtaga agcagtacgt
    31441 aaagaaacag gaaaagaacc aaacaaatca gttaaccctg atgaagtagt tgctatgggt
    31501 gctgctatcc aaggtggcgt gatcactggt gatgtgaaag atgttgtttt acttgacgtt
    31561 acaccattgt cattaggtat cgaaacaatg ggtggagtgt tcactaaatt gattgaccgc
    31621 aatacaacga ttccaacaag taaatcacaa gtattctcaa ctgcggcaga taatcaacca
    31681 gcagtagaca tccatgtatt acaaggtgaa cgcccaatgg ctgctgacaa caaaactcta
    31741 ggaagattcc aattgacaga tattccagca gctcctcgtg gtattccaca aatcgaagta
    31801 acattcgata ttgataaaaa cgggattgta aatgtatctg cgaaagatct aggtacacaa
    31861 aaagaacaaa aaatcacgat caaatcttct tcaggtctaa cagacgaaga aatcgaacgt
    31921 atggtaaaag atgcagaagc aaatgcagaa gcagataaag ctcgtaaaga ggaagttgac
    31981 ctacgtaatg atgtagatgc attgctattc tctgtggaca aaacattgaa agaattagaa
    32041 ggcaaagtgg acgaagaaga agtgaaaaaa gcagaaacag ctcgcgatga attgaaagcc
    32101 gctgttgaag caaacaacat cgaagaaatg aaaactaaac gcgatgcatt gaacgaaatc
    32161 gttcaaaact tgactgttaa actatacgaa caagcggcac aacaacaagc tcaagaaaac
    32221 ccagaagcag ctcaaggcgg agcagatgat gtcgttgatg ctgattttga agaagtcgat
    32281 ggcgacgata aataatcagt tcactgaatt tcgagagaag gccaaagttg ctactttggc
    32341 tttttctttt gaaaaatagt ttgaagtatc ggtttttgtt tcaaacaaaa tatgatatga
    32401 taacaagcga cgtgaaaatg atgagaacca ataggaggaa agcctatggc aacaaaacgt
    32461 gattattatg aagtcttagg gctctcaaaa ggagcatcag aagatgaaat aaagaaagct
    32521 tatcgtaagc tttcgaaaaa ataccatcct gacatcaata aagaacctga tgcggaagaa
    32581 aaattcaaag aagtatcaga agcatatgag attttaagtg acccgcaaaa acgcgctgca
    32641 tatgaccaat acgggcatgc aggcacagat ccaaattacg gtgctggtgg agctggtggc
    32701 ggatttggtg gattcggcgg cttctctggt ggcggcttcg gtggttttga agatatcttc
    32761 gactctttct ttggaggagg cggtcggtca gtcgatccaa atgcgcctcg tcaaggtgct
    32821 gatttgcaat atacggtcga cttgtctttt gaagaagcga tttttggtgt agaaaaagaa
    32881 atcaagtata atcgggaaga aatctgtcat acttgtggtg ggaacggtgc aaaaccaggc
    32941 acacaaccaa ctacttgtca taaatgtcat ggtgcaggta cgattaatgt agaacgccaa
    33001 acaccacttg gacgagtgat gagccgtcaa acttgtgatg tttgtcatgg gacagggaaa
    33061 gaaatcaaag aaccttgtcc aacctgtcat ggctcaggac atgagaagaa agcacattca
    33121 gttaaagtga atgtaccagc tggtgtagaa gaaggacaac aaatgcgttt agctggtcaa
    33181 ggagaagctg gagaaaacgg cggaccattt ggtgatttgt atgtggtctt ccgtgtagaa
    33241 gaaagtgata tctttgatcg tgaaggttct gaaatttact atgaattgcc tttaagtttt
    33301 gtacaagcag ctttggggga tgaagtccaa gtaccaactg tccatggaga tgtgaaattg
    33361 aagatccctg ctggaacaca gacaggtacg aacttccgtt tacgtggaaa aggcgctccg
    33421 aagttacgtg gtggtgcaac aggcgaccaa catgtcaaag taaaactgat cacaccgaaa
    33481 aatctaaatg aacaacaaag agaagcatta cgtgcattcg ctgaagctgg cggcatcaaa
    33541 gttgaagaac agcaagaaga cggtttcttc gataagttta aagatgcttt tggcgggaaa
    33601 aagcggaagt agtttttcta gtaaaaacgc taagtcatta ataattaaaa accagacgaa
    33661 caatcatatg ttcgcctggt ttttttgtag ttgtcatgtt tgtaccaaca aaaaagccag
    33721 ctttagaata tgataatcgt catagtttct aggggtataa ccagtgagag aatggaactt
    33781 gttcaatcga tattgcaagg tgtttttatg aataaataat tcttctgagc aatttttgat
    33841 gcttccgtta tttttactga agcatgccaa agtcttcttg aacaaatgta cttcttcttc
    33901 tgtcaggctt ttcaaaatac gttttacata taatgttcgt tgatctgacg ggattgctgg
    33961 taaaacgatc ccgaggtcta aatcttcaaa aaagcggatc tcgttcacag attgatggaa
    34021 agctatccat tcgcaaactt gtttggcatg aaggaaagat tgcggtgtct cttgctctgt
    34081 ataacaaagc gacccaattc caaagtttag ctgtaactga tacattttat taaactttgt
    34141 ctggatttta tcaagcaatg atagcaatga ggctctggaa atccgattga ctaggataat
    34201 gatttgttgt ctatgtacag tgagcaaagc ctgtccgtaa taagagaaga ccctttcgag
    34261 caactccaat aaaggattga tccgttcttg acacaattgc tgattgtcag aaagttgtcc
    34321 cacaactaca taatatggag aagaaaaatc aagctcaatt aagtgagggt ggtatcgact
    34381 atgtacttga tatcatcaat gaaccgatgg tactttctta tgcgatggaa caggtagagc
    34441 gattgcttgt ttgtgcagga aaacaagcga tgcggctgat acgattaggt gagaaaataa
    34501 gaagataaaa atacacggtc ccttcatggc gaccgtgcat ttttggtttt tattaaattt
    34561 tatatctaat tttcaattat ccgcataaac actatacatt caagcatttt gaagtataga
    34621 aaaacactca tcttaccacg aatttaccac gattgaatga gccttgatat gaccgtaaaa
    34681 ctactaaggg agataacacg aaactgtgta tctcccgatt tttttataca gttgattact
    34741 tcactccgat ttcaaatttc aatattgctt tcatcatgtc tgtatgtatt tctcctttta
    34801 gttcttcatc aacctgcata atgattttac cactttcaga gtggtaaaaa ggacgcaagc
    34861 ataatttaga aatataaccg tcataaaact tcaaaatctc tttaactgcc atttcatctc
    34921 cgcttgacgc tttgcaaatc aattcaaaag agggatagcc ataatcttta cttcctttcg
    34981 ctcaaaagca tttttaactt ccttagtcct ctagttcttc tactgcaaat agcagaacgt
    35041 gatacatggt acagttctga aatttctctg tcgttcattc cttgaaaata acgtaataaa
    35101 atcgcactac gctgtttttc tgatagtcta tgtaaaactg taccaagtgt ttcatcagat
    35161 acataaattg taaagtgcaa gactttaaat gatacacaat cataaaaaca ggtatcaatc
    35221 gttccacgtt caacttgttt cgtttcggct atttcacaaa acaatacttc acgtttagaa
    35281 tgtcttgcaa gctgtctgtt tcgacttttc actttgttct taatgacaat cagcattaaa
    35341 gaattgaact gtaatctgac gatatgctcg aaagaagatg tcctcataat ctcaccccct
    35401 ttctgtctag gaaaggaagt gagccttgct cgtttatctc ctttcactct tagtcccaat
    35461 gtgaaagggg gatttgttgc attactgata aataaacttt gtaaaaaagt tctgaatagc
    35521 caaaaaagca tataaacaga tttatttctc tgtttacatg cttctgttat tctatctata
    35581 tgatttataa aaccacattg gtggacgtac ttatctattg cagatagacg actttttttg
    35641 acaagaaccc aatgtaagga aatttattgt atatgatgta cttcatggcg acgttgacct
    35701 ccaacaaacc gccatttgga agtaatatac aatattttaa cagcgtaaat agcactacca
    35761 tataacggtt ttttttattg gcgtttagta gtgcttttta ttaaatataa acctataaac
    35821 catataacac gtttttctat acctgttttt aattcagtag gaacaataaa atgtatagag
    35881 gtggtctact atgcgtaaaa aagaagataa atatgatttt agagcctttg gtttagccat
    35941 taaagaagct cgattgaaac gaggtttaac tcgtgaacaa gtgggagcat tgattgaaat
    36001 tgacccacgg tacttaacta atattgaaaa taaagggcaa caccccagca tacaagttct
    36061 ttatgacctt gtatcgttac ttcatgtttc cgttgatgaa tttttcttac ctgctaataa
    36121 cttggtaaaa agcacccgac gattacagat agagaaatac atggatagct ttacagacaa
    36181 agaactatcc ttaatggaat ctttagccag cggtatcaac gaagcaagaa acatcgaaga
    36241 ctaattaaaa gaatccatac ataacggaaa gagccgataa aatgagattg tattaatctc
    36301 attttatcgg ctctgcgtct ttgcgtctgg ctctgtaatc acagttactt tgaactgctt
    36361 tatttcaatt aaattttctt gtctgcattt cggacaatag agggggaatt tttttaattc
    36421 agtatcttcc cttatcttta atcgtgtttt atttccacat acaggacaca atatccactt
    36481 gtagtttata ataactatct cctcctttac actttaattc aaatctttat taaaaaatat
    36541 ttcatcttat ttaacaagaa accatattta tataacaaca taaaatacac taagttattt
    36601 tattgaacat atatcgtact ttatctatcc gactatttgg acgacggggc tggcaaacag
    36661 gttcaccggt agtaacatgg taccctttta actctgttaa acaaacacta cgtccatttg
    36721 taaagaaagt taaatcacta cgatattctt gaatacaccg agcagggatt tctccactaa
    36781 gaatgacctc attatttttc aattgagtgt ctacgatgtt cgcacaatat ttaggagcat
    36841 cgttgtatgc tcgtgaaaga tattcctgtg gcgcataaat tttaaaacta agatatggct
    36901 ctaacaattc tgttccagct ttttttaaga cttgttccaa tacaatagga gcaagcatcc
    36961 gaaaatctgc tggggtacta acagggctat agtataagcc atacttaaaa cagattttac
    37021 agtccgtcac attccaacca tacaatcctt gttcacagcc atagcgtatc ccctccataa
    37081 ctgcattttg aaacgattga tttaagtatc caagagaaac cgagctctca tactgcattc
    37141 cacttcccaa cggaagcggt gatacagata aaccaatgga agcccagaaa ggatttggcg
    37201 gcacttcgat gtgaatggta tattctgcat tttttaacgg tctctccata taaatgactg
    37261 taggctcttt tagttctatc tccacatgat acttttcttg caacagtgca ctaatcactt
    37321 ccatttgtac tttccctaag aaagaaagta taatttcatg tgtcgtagaa tccacgtaat
    37381 atcgtagaag cggatcacta tctgagattt ccaaaagggc atcaagcaac atttctctct
    37441 gttcaggttt actcggttca acagttgttt gtagtagagg gtgcggattt tcaatctttt
    37501 ttctctgtgg caatagtttt gtatctccaa gaacactatt taacttcaaa aactcatttt
    37561 gcaaaataac aatttctcca gaataagctc tatcaatctt acataattca ccatttattg
    37621 aagtatacat ttctgtaact tttatttttt ctttttctga tactctaacc gaatctcgta
    37681 aatgtagtac tccactataa aggcgtatat atgcaagacg ttgtcttttt tttgtatatt
    37741 caattttgaa aacatttccg caaagttcag acggacctcg atgtgttgat gaataaaatt
    37801 tattagtaat aacttctata aggttatcaa tccctatatt actttttgca cttccatgat
    37861 aaagagggaa cagagaacaa ttctgaaatc ttatgctttc ctcttgttcg agttccaatg
    37921 cttctaatga tttaccggac atatatttct ctaaaaggtc atcgtttccc tctattaccg
    37981 tatcccattg ttcagattcg gtaaagttcg tcacacacat attaggatac agttctacct
    38041 tctgtttgat tacaatttcg gcagaaagtt tctctttaat atcctgataa accgttgata
    38101 aatcaattcc attttggtca atcttattga taaaaaagat tgtgggaatc cccattttcc
    38161 taagtgcatg aaataatata cgagtttgtg cttgtacgcc atcttttgca gaaatcagta
    38221 gaattgcccc atctaaaact gataatgaac gatatacttc tgctaagaaa tccatatgtc
    38281 ctggcgtgtc tatgatgttc accttcgtat tttcccactg aaaagaggtt attcctgtct
    38341 gaattgtaat tcctctctga cgttctaaaa gcgtattatc cgtcctcgtt gtacctttgt
    38401 ccacgcttcc taattctgta atcgctccac tgttatataa taagctttct gttaaggtag
    38461 tttttcctgc atcaacatga gctaaaactc caatattaat aattttcatg tgattttcct
    38521 ccattcaaaa acccaaaagg gcataaaaat cccagtgata aatactttta tcactgggat
    38581 ttttatgcat aaccataggc atacaaagca tacagatatt ctccggatac tttagaatca
    38641 catgataaag gtattcttaa actgggtaca aaaaactaag ccctcctaaa aaaggacatc
    38701 caattatttg ttcccactat caaattgaca gtttatttaa gaataccttg ccgcatattt
    38761 attaactcct tttaaataga tacttaaata atagcacgta agagcatatt tgtaaaggaa
    38821 tctccaattt tttatcaaag agagtacgtg attacaaaat agctgtaata atgtaccaat
    38881 atttgttatt ctataatctt ccaattactc ccgttctttt caagtaccaa atcaaattga
    38941 gatacctgcg ttgcctttgc ctgatttacc acaaatcaga ttttatccct ttcttcctct
    39001 tgattgccca caacagaaaa gaaaaagccc tgtcaagagc tttgccacca ttggtggtgc
    39061 tttgcactct tgatcgggct ttttgtttgc tgtatcttct cgcaagagga aaaaagttac
    39121 tcgtcctcat catcaaaatt gttattcctt aatacttctc gtaacagttc catatcttct
    39181 tttgagttcg ggtttatcat tttcacgact tgataaggtg tcttatactt atgtccctct
    39241 atgctttccc caaacatatc catactgtat aaatcatcat agcggtctaa taatttttga
    39301 aacttcaaat gctggataaa ttctttgatt gggtaaaggt cggacgcttc aatgcttttc
    39361 ccgttgatat agtctgtaat atattcccgt aacttttcta actcatattc cagccattct
    39421 tcctcgctgt caaagaagtt atcatagtgt ccatgtttca gttctgcatt tagacgttct
    39481 tcggctctta aaaactgaca gacttccttt tcagattgat tgacatactt ccattctccc
    39541 gacaacaatt cattgggcgt aacgtccagc acttccatta tcttttccag cgtttcatag
    39601 gcaggatagt tcaaccctct ttcaatcttg gaaaggctct gcatattgat accgattttg
    39661 tccgcaagtt cctgttgttt cattccccta aattttctta tggtctgtat attatttcct
    39721 aaatggctta ttttcattgc ataaccctcc atatctcgtt atatcttcgc tattataagt
    39781 ctatcatgct taatatgatt tgtaaaggca aaaaaagata ttgacaaata agcatataag
    39841 aattactatg ttcttgtaat cgaataagca caatagaatg aaatataaaa acattcaaaa
    39901 agaaatacct atctgattaa atccgtagat atgaatatcg gttgaaatgg aacttgttag
    39961 aaaaggcaaa acagaacttg ctgacaaagt atttctgaca cggtggcgga agcctctcag
    40021 gcttaccgcc atgacacctt gtgtcaaggg gaagaaatgc tttgtcggct ggcgagcctg
    40081 cgagccttgt ctgtgccccc gttatctaac aggggggcac tatttcacaa aagacaagtc
    40141 aaaaaccata caatcccaaa gttgtgagta gtaatgagaa gttttaagaa gatagtgcat
    40201 atcacacttt ttgaatagag gtttacaggt atttcaaggc tattgtggga ttggaggaat
    40261 acaatgaaca atttagaatt tgcacttgaa atgaagaaca aacgtcttgc aagcggtctt
    40321 tcacaaggag aacttgcaag tcttacccat gtatcaagat atagtatcaa ccgctttgag
    40381 aacggaaaag ccaacgccag caaggaaaca caaaatatta tcctgcgttg tctgaattat
    40441 tatatctgcg ataagccttt ttatctgctc gttgattatc tgtctgtccg ttatccaacg
    40501 actgacgcat tggaaattat ccgaaaggtg cttggcatga aagcagatta ttttatccat
    40561 tatgactatg ggtattatgg ctataaagag cattacgcct acggagaaat caaggtcatg
    40621 gcttctgatg acgaacacat gggcgtattc ttggaattaa aaggggcagg ctcacgcaac
    40681 atggaatatg tcttgcaggc acagaacaga gattggtatt catttttaaa ccgctgtctt
    40741 gactgtgacg gtgttatccg acgttttgac ttggcaatca atgatatgtg tggtctgttg
    40801 gatattcccg ttttgtctga aaaatacaga aatggcggtg cggattgtcg ctgtaaaaat
    40861 tatgaaaatg tgcagggtgg aaaactaagc ggaaaaaacc gtaatttagc aagcaccctt
    40921 tatatcggtt caaaggcaag cacaaaatac ttctgcctgt atgaaaaaca aaaggaacag
    40981 gcaaccaaaa agaaacatac ggatattatc aatcgttttg aaattcgtct gcgtggtaca
    41041 aaagcagtac aggcagttga ggaattgtta ttgacatata accctcatgg gctggtgttt
    41101 taccttatta ctgattttgt gcaatttccc gactatccgc tgtgggagat attcatttcc
    41161 catgacagtt taccttttga aatgaacccc gtacctgtca atatggaacg cactctgcaa
    41221 tggctggaaa gacaggtcat gccgtcaatc gtgatgatag aggaaatcga caggctgaca
    41281 ggctcaaact acatgaaaat gattgatgaa tgtacccacc tctctgaaaa gcaggaaatg
    41341 cttgtggaac agatgtgtac ggacatagca gacgtaatcg aaagtgaggg ggtgttttat
    41401 gagtaatgca caggatattc ccgtatggga aaaatacacc cttaccattg aagaagcgtc
    41461 aaagtatttc cgtatcggag aaaacaagtt aagacgattg gcagaggaaa acaaggacgc
    41521 tggctggctc attatgaatg gcaaccgcat acagattaaa cgccgacagt ttgaacaggt
    41581 cattgataaa ttggacgcta tctaatgcaa atgagccttg tatgtgttat gatgaacaca
    41641 agtcatatca aggctctttc caacaaggaa aggagcagac accatgaaag aaaaaagacg
    41701 ggataacaaa ggtcgtatcc tgcatactgg agagagccaa cgaacagaca gaaaatactt
    41761 atataaatat gtagacgcat ttggaaacac aaaatatgtg tatgcttgga gattgacacc
    41821 cacagacccg acaccaaagg gaaaacggga aaaaccctca cttcgagaac tggaacagca
    41881 gataagacgg gatattgagg acggtatcga cagcactggc aagaaaatga cgctttgcca
    41941 actttatgcc aaacagaacg cacagagggc aaatgtgaag aaaagcacac agaaacaacg
    42001 ggaacaactc atgcggttat tgaaagagga caagttaggt gctaggagca ttgatacaat
    42061 caaaccctct gacgctaagg aatgggcgtt acgcatgaaa gacaaaggct tttcctacaa
    42121 taccattaac aaccataaac gctcgttaaa agcgtcattc tacatagcca ttcaagatga
    42181 ttatgtgagg aaaaatccct ttgatttcaa gttgagtgaa gtcctagaaa atgataccaa
    42241 aaagaaagtc gcattgacag aggaacagga acaagcctta ctctcattca tcaagacgga
    42301 caatgtgtat cacaagcatt atgatgatgt gctgatactg ttaaagacag gacttcgtat
    42361 ttcggaactg tgcggactga cagtagccga cattgatttc aagaatgaag ttgtgattat
    42421 cgaccaccag ttactaaaga gcaaggaaca gggctattat attgaaacgc ctaaaacaaa
    42481 aagcggaata aggcaagtac cattaagcaa agaaacgata caggcatttc aacaggttat
    42541 gaaaaaacgt ccaaaggcag aaccatttgt gatagacgga cggggcaact tcctatttgt
    42601 caatcataag ggcaagccca aagttgcgat tgattataac gccttattta tccgtatggt
    42661 aaagaaatac aacaagcacc acaaggacaa tccattgcca catatcacac cgcatacgct
    42721 acgccataca ttctgcacaa gactggcaag caagaacatg aacccaaaag atttacagta
    42781 tatcatgggg cattcaaata tcagtatcac aatgaactgg tatgctcatg cgtccataga
    42841 taccgcaaaa tcagaggttc agcgtctaat tgcatagaag tatttaccac gattttaacc
    42901 acgcttgata gtgaaaatat aagaagatag acctagatat gtgaggttta ccacaaaagc
    42961 aaaatgcccg tagagcctat aaaataaggc tttgcggaca tttaagaaga tataaaaaga
    43021 tagtcaaaaa gacatatata atttatttta aatatttaat actttattca agaaatcttt
    43081 tgtccgttca ttttgcggat tttcaaagat ttgttgaggc gttccatctt ctaaaaagtt
    43141 tccgtcgtca atgaacatga cgcgattggc cacttcttta gcgaacccca tttcgtgcgt
    43201 aacgataacc atcgtcatac cttgttctgc taactttttc atgacgttca agacatcacc
    43261 gaccatttca ggatcgagtg cagaagtagg ctcatcaaaa agcatgatgt cagggttcat
    43321 cgctagagca cgagcaatag ccacacgctg tttttgtcca ccagaaagca tttcaggata
    43381 catatctttt ttatcttcta gaccaactgt ctgcagtaat ttgattgctt ttttctctgc
    43441 ttcttctcta gattctttct tcaggtccat cggtgctaaa atgatgtttt ccaaaataga
    43501 aagatgaggg aataggttaa agtgttggaa taccattccg atatgttgtc tcaccaagtt
    43561 gatatctgtg cttttatcca tcaagtgggc gccgtcaata ataatttcac cgtcagaagg
    43621 ttcttctaaa cgattcaagc aacgcaagaa tgtacttttt cctgaacctg aagggccaat
    43681 gatacagacg acatctcctt cgttgatgga tacattgatg tcgtttaata ctgtgttatc
    43741 gccgtatttt ttgactaaat gctcaactaa gattttttct gccatcttac ttcaccttct
    43801 tttctaaaac ttttgctaat tttgtcaatg ctgtgatcaa aatcaagtac atgatcgcaa
    43861 tgatcaagta aacatacgta ctttgtgttg tacgtgcaac aatgatctta ccagtttgca
    43921 gcaattcgac tacaccgatt gcggaaatga tcgttgtgtc ttttaatgag ataacgaact
    43981 gattgacgaa tgatgggatc atgatgcgta tcgcttgcgg caaaatgatt ttttgcattg
    44041 tccgattata agaaagacct aaacttctgg aagcttccat ttgtccgaca ggaacggcgt
    44101 tgatcccgcc tcgaacaatc tctgcgatat aagcacttgc gttaagagtc aaggtgataa
    44161 caccagcaag gaagtcagga atcgtaaatc caacgatacc aggcaaccca aagaaaataa
    44221 agaacgcaag aaccatcatt ggaattcctc ggatgatatc gacataaata ctagcaattg
    44281 tgcgaagagc gcgtattggt gctacactga ataatccaaa aatcacaccg attactatag
    44341 ccaaagcaaa ggagatcaaa gttaggagaa ttgttctcca aagtcctttc aacaatgctt
    44401 gatagttgtt ttggataagt ccaagaaatg ttgattcatc agcagtggaa gactcactgg
    44461 aagtttctga accatccgct acatattctt taataatctt atcgtattcg cctgttcttt
    44521 ttaattcttt caatccttca ttgaacatat taagaagctc aggattttgt ccttttttaa
    44581 cggcaaatgc atattttccg ccagattctt ttttgatcgg tgtttccaat tcttgtcctt
    44641 gtttgatcgc ataaccgatt accggataat catccatcat tgcatcgatt gcgccaactt
    44701 ttaacccgtc gtacaattga tcagcagcat caaaatattt gatagaatag ccatattcag
    44761 attcatggtc gcttaaaaat tcagcacttt cggtaccaac ttttgctccg actgtttttc
    44821 ctttcaaatc ttcataagac ttgatcgaag tatctccttt tttgacagct aattggatcc
    44881 cgctatcaaa gtaagaatca gaaaaatcat agcttttctt tcgttcatct gttacagtca
    44941 ttccggcaat cattccgtct gcttgtcctg attccaaagc ttgcaaagca gcagagaaac
    45001 cacggtagtc cattcggatg ttaaatcctt gcagtttggc gatgttttcc atcaatttta
    45061 catcgatgcc ttcgtactcg ccctttgcgt tttgaaattc aaatggagca aaagcagagt
    45121 cactggcaat tacgtatacg tcttttttgg gctctatttt ttgcattgtc tcgcttgtgc
    45181 ttgactctgc cgttgtagtg ttgtcagctt gtgcaacatg accaaacgca agtgtcacaa
    45241 gtcctgtcat taagaaaaca aatgagtgaa ataaatgctt tcttttcata atcaacctcc
    45301 taaatttcca atattttatt attataggcg aaaagtgctg aatcagacaa gactgtaagc
    45361 tcgaaaaaat gaaaaaaatg cgagtttcta acattttatg ttataaaaca taacatgtat
    45421 aataagataa aaaagtggcg aaagtatgtt cggtgtggta taatttttta gtaaggaatg
    45481 cctttgcttt ccttttcttt ttattggtcg taaaatgaaa aggaatgaat gaaaaggagg
    45541 acgaagaatg attgagagag atacctcccg acactttgag cttgtctcga aataccaacc
    45601 ggctggtgat cagcctgaag cgattaacca attagtggat ggcgtagtcg gcgggaaaaa
    45661 agcacaaatc ttattaggtg cgactggaac agggaaaaca tatacgattt ccaatcttat
    45721 tgagaaagtg aataaaccaa cactgattat tgcccataac aaaacattgg ctggtcagct
    45781 gtacggggaa ttcaaggaat ttttccctaa caatgctgtt gaatattttg tttcttatta
    45841 tgattattat cagcctgaag cttatgtgcc ttcaagcgat acgtatatcg aaaaagattc
    45901 aagtgtaaat gatgagatcg acaaattacg acattcggct actagttcat tattagaaag
    45961 aaatgatgtg atcgtcattg catccgtttc atgtattttt ggattaggct cgccttttga
    46021 gtaccaaaaa caagtagttt cgattcgaca gggagcagaa ctagatcgca atcagttgat
    46081 tcgtgactta gtcagtatcc aattcgaaag aaatgatata gattttcagc gcggtcgttt
    46141 tcgagtgcgc ggtgatgtcg tggagatttt tccggcttca agagatgaac gtgccttacg
    46201 tgtggagttt tttggagatg aggtcgaacg tatccgagaa gtcaatgcac tgacaggtga
    46261 agttttagga gaaacggaac acgtagcaat cttcccggcg actcactttg tgacgaatga
    46321 tgaacatatg gagcatgccg tagcgaatat caaagaggaa ttagaacagc gtttaactgt
    46381 cttgcgaaat gaaaacaaac ttctggaagc tcaacgttta gaacagcgaa ccaattacga
    46441 tattgaaatg atgttagaaa tgggctacac gtcgggaatt gaaaattatt ctcggcatat
    46501 ggacggacgt aaagaaggtg aaccgcctta tacgctattg gatttctttc ctgaagattt
    46561 tttgatcgtg gcggacgaat cgcacgtaac catgcctcaa atcagaggaa tgtataatgg
    46621 tgaccgagcg agaaaacaga tgctagtaga ttacggtttc cgtttgccat ctgctttaga
    46681 caatcggcca cttcggttag aagagtttga aaaacatgtc aatcagatca tttatgtctc
    46741 tgcgacgcca ggaccatatg aacatgaaca aacagatacc gttatccaac aaatcatccg
    46801 tccgacaggg ctgcttgacc cagtgatcga agttcgtcca atcatgggac aaatcgatga
    46861 tctagtaggc gaaataaatg aacgagtcga aaaagaccag cgggtgtttg taacgacatt
    46921 gacgaaaaaa atggcagaag acctcacaga ttatttcaaa gaattaggga tcaaagtcaa
    46981 atatttgcat agtgatatca aaacgctgga acgaacagaa atcattcgag atcttcgatt
    47041 aggtgaattt gatgtgttag ttggaatcaa tttgcttcgt gaaggattag acgtacctga
    47101 agtttccctt gttgctatct tggatgcgga taaagaaggc tttttacgta gtgaacgttc
    47161 cttagtccaa acgattggac gagctgctcg gaatgaagaa ggtaaagtaa tcatgtacgc
    47221 agacaaagta acagattcta tgcgtctagc gatggatgaa acgtcaagac gtcgtacgat
    47281 tcagcagaag tacaatgaag aacatgggat cgttccaaaa acgatcatta aagaaatccg
    47341 agatttgatt tctattacga aagaatcgga agatgataca aaagaagcgg tacaagtctc
    47401 gtacgaagaa atgacgaagg aagaaaaaga cacgttattg atgaaattag aaaaagaaat
    47461 gaaagatgcg gcaaaagctt tggattttga aacagccgcg aatgtgcgag atatgatttt
    47521 agaattaaaa gcttcaaagt aggaggacaa catggctaac gataaaatta cgattcatgg
    47581 tgcacgtgcc cataacttga aaaatataga tgtaacgatt ccacgagata aattcgtggt
    47641 cgttacaggt ttgtccggtt ctggaaaaag ttccctagca tttgatacgc tgtacgctga
    47701 agggcagcgg agatacgtag agagcctttc tgcttatgct cgtcagtttt taggacaaat
    47761 ggataagccg gacgtagata gcatcgacgg attgagtccg gcgatttcta ttgatcagaa
    47821 aacaacaagt aaaaatcctc ggtcgacagt aggtactgtg acggaaatca atgactattt
    47881 acgcttgttg tttgcacgtg taggacatcc gatttgtccc aacgatcata tcgagattac
    47941 cagtcaatca gtagatcaaa tggcagatca agtaatggct ttgcctgagc gcacaaagat
    48001 ccaagtgttg gcaccgatcg tggtaaagaa aaaaggacaa cataaaaaaa tattcgaacg
    48061 aatccaaaaa gagggatacg tccgcgttcg agtcgatggt gagatctatg atttaagtga
    48121 agtgcctgag ctggagaaaa acaaaaaaca tgatatcgcg atcgtcatcg accggatcat
    48181 tgtgaaagaa gggattcgtt ctcgcctatt tgattcttta gaggcggccc tgcgattagc
    48241 agacggctat gctctggttg acgtgatcgg tgaagaagaa atgttgttta gcgaacacta
    48301 tgcctgtcct tattgcggat ttactgttgg cgaactagaa ccaagactgt tctcattcaa
    48361 tgcgcctttt ggagcttgtc cagactgtga tggtttaggt gtcaaattag aagtcgatca
    48421 agatttagtg attccagata gaacgaagac acttagtcaa ggtgctatcg ttccatggaa
    48481 tccaatcagc tctcagtatt atcctcaaat gttagcgcag gcttgtgaaa gtttcggtat
    48541 cgatttagat acaccattcg aagaattacc ggaagagcat caggagatca tcttgaatgg
    48601 gacagatgat cttttccatt tccattatga aaatgatttt ggtggcgttc gcgatgtaga
    48661 aacgactttc gaaggggtat tgaagaatat cgagcgtcgt tatcatgata cgaacagtga
    48721 tttcacgcgt gagcaaatgc gtctttatat gacagaatta acttgtcaga catgtaaagg
    48781 ttatcgattg aatccccaag ctctatctgt tcaaatcaat ggaacgaata tcgggcaaac
    48841 aaatgagttg tctattcaaa aagcagtaga tttcttctca aacttaacgt tatctgaaca
    48901 agagaaagta attgccaaac cgattttaaa agaagtaaat gacagacttt cattcttgga
    48961 aaatgtggga ctagactatc taactttgag tcgcgcttcc ggaacattat ctgggggaga
    49021 agcgcagcga atcagattgg ctacacaaat tggatcgaat ctttctggag ttttgtatat
    49081 tttagatgaa ccatcgatcg gattgcatca acgcgacaac gatcgactga ttggctcatt
    49141 aaagaaaatg cgtgatcttg gcaacaccct cgttgtagtc gaacatgatg aagatacgat
    49201 gtgggcagca gattatttga ttgacgtcgg acctggggcc ggagaacaag gcggacaaat
    49261 cgtggctgca ggcactcctg aagaagtagc taatgatgaa aattctttga cagggaaata
    49321 tctttcaggg aaaaaatcaa tccctgtacc aaaagaacgc cgtaaaggta atgggaaagc
    49381 aatcaagatc actggtgctt cagaaaacaa tctgaaaaat attagtgttg aatttccatt
    49441 aggagaattt gtagcagtca caggtgtgtc tggttctgga aagagtacat tggtcaatag
    49501 tatcttaaag aaatcgttag cgcaaaaatt aaataagaat tctgctaagc caggtaaatt
    49561 caagacaatt tccggctacg aaagtatcga aaagatcatc gatatcgatc aaagcccaat
    49621 cggccggacg ccgagaagta atccagcgac ttatacaagt gtatttgatg atatccgtgg
    49681 gttatttgct caaacgaacg aggcaaaaat gcggggttat aagaaagggc gttttagttt
    49741 caacgtaaaa ggcggtcgtt gtgaagcttg tcgcggggat ggaattatta agatcgaaat
    49801 gcactttttg cctgatgtct atgttccttg tgaagtatgt catggtaaac gatataattc
    49861 tgaaacatta gaagtgcatt acaaaggaaa aagcattgct gatattttgg aaatgacagt
    49921 agaggatgct gtagaattct tcaagcacat tccaaagatt catcgcaaac tgcaaacgat
    49981 tgttgatgtt ggcttaggtt atgtgacgat ggggcaacca gcaacgacat tgtccggtgg
    50041 tgaggcacaa cggatgaaac ttgccagtga attgcacaaa atctctaatg gaaagaattt
    50101 ctatatacta gatgaaccaa cgacaggact tcatagcgat gacatcgccc gcttgttgca
    50161 tgtattacaa agattagtag atgctggtaa cacagtttta gtgattgaac acaatctaga
    50221 tgtaatcaaa acagcagatt atatcattga tttaggacca gaaggtggag aaggtggagg
    50281 aacgatcctt gcgactggaa caccagaaga aatcattaac gtaaaagaaa gttatacagg
    50341 tcactatttg aaaaaaataa tggtataaag aggatagata gctgagatag ttaaaaaaac
    50401 ataaaaaaat gaatttttgc ttgttttatt aatatcagtg ttctagaatc attacatggt
    50461 agttctttct acactgattg gtatagataa aaacttccta taagttcaat ccgttcaaaa
    50521 aagaattgag caatcggagg actttccttt gattgctttt ttctttatgg tatgatattc
    50581 cttgtgtagt tggaaaagaa aaacaagaga ttatgagtaa gtgagagata atgatgaaca
    50641 gtattgaaaa tgaagaattt atccctatat tattaggaag cgacatgaac gtttatggta
    50701 tggcacgttc ttttaatgaa gcatacggca aaatctgcca agcatatgct tcggatcagt
    50761 tagcccctac ccgctacagt aaaattgtca atgtagaagt tattccagga tttgataaag
    50821 accctgtatt tatcgagacg atgcttcgtt tggcaaaaga aagatattct gataagagta
    50881 aaaaatatct tttgattgct tgtggtgatg gatatgcaga attgatctcc caacataaac
    50941 aagagctgtc agaatatttt atctgtccat atattgatta ttctttattt gaaaggctga
    51001 tcaataaagt aagcttttat gaagtttgtg aagaatatga tcttccgtat cctaaaacat
    51061 tgatcgtcag agaagaaatg ttagtaaatg gacatttgga acaggaattg ccttttgaat
    51121 tcccagttgc tttgaaacct gcaaacagtg tggagtatct ttctgtccaa tttgaaggaa
    51181 gaaaaaaagc ttttatttta gagactagag aagagttcga tttaatttta ggccggatat
    51241 acgaagccgg ttataaaagt gaaatgattg tacaagattt tatcccgggt gatgatagta
    51301 atatgcgtgt gctaaatgcg tatgtagatg aagatcatca agttcggatg atgtgccttg
    51361 gtcatccact attagaagat cctacaccag catcgattgg taattacgtt gtgatcatgc
    51421 cagattataa cgaaaaaatc tatcaaacca ttaaggcatt tttggaaaaa atcgaatata
    51481 caggctttgc caatttcgat atgaagtatg atcctagaga tggagaatat aaattatttg
    51541 aaatcaattt gagacaaggt cgtagcagtt tctttgtgac attgaacggc ttgaatctgg
    51601 ctcgttttgt cacagaagat cgagtgttca acaaaccctt tgttgaaacg acctatggaa
    51661 cgaatcaatc agataaagca agattatgga tgggtgtgcc caaaaaaatc tttttagaat
    51721 atgctcggga aaatgaagac aaaaaactag ctgagcaaat gatcaaagaa aatcgttacg
    51781 gtactactgt attttatgaa aaagaccgat caatcaagcg ctggttgttg atgaaatata
    51841 tgttccataa ctatattcca agattcaaaa agtattttca tgtgaaagaa ggctagttca
    51901 tggagaattt tttcagtatt ttaggcggaa tgggcacgat ggcgacagaa agttttgttc
    51961 gcttgatcaa ccaccgaacg aaagctacaa aagatcaaga atatttgaat tatgtcttat
    52021 tcaatcatgc aacagttcct gatcgtacag cttatatttt agatcgatca gaagaaaatc
    52081 caatgccgtt tctgctggat gatattgaga aacaaaattt attgcgacca aacttcattg
    52141 tgttgacttg taacaccgca cactactttt ttgaagagtt acaagcggca acggatattc
    52201 ctatcttgca tatgccaaga gaagcagcaa atgaacttgt ccgtcagcat acgacaggga
    52261 gagtagcgat tctaggaaca gaaggaagta tgaaagctgg aatctatgaa cgcgaagtca
    52321 aaaatcttgg tttcgaaaca gtgattcctg atactgcgct tcaagaaaaa atcaattacc
    52381 tgatctatca tgagatcaag gaatcggatt atttgaatca agagctctat tacgaaatct
    52441 tagaggaagc ggtagagcgc ctaaactgtg aaaaagtcat cctaggttgc acagaattat
    52501 ctttgatgca tgaatttgct gaagataatc attacccagt gatcgatgct caatctattt
    52561 tagcggatcg aacgatcgaa cgtgcattag ctgaaagaag cgaggcattg gatacagcat
    52621 cggaaaagta aaaaaattgg atttggggca aatatcctaa aagatatttg gtccaaatct
    52681 ttttgtaaaa gaaaaagaaa actggtaatg atacgcccgg gatgatataa ttttattatg
    52741 cacaatgtga gaggggttcg taaatatgac agataatttg caattagtca tcataacagg
    52801 tatgagtggt gcagggaaaa cagtagctat ccaaagtttt gaagatatgg gctatttttg
    52861 tatcgataat atgccgcctc gattgattcc aaaattctgg gaattgatca aagaatcagg
    52921 taaagtcaca aaaatggctt taggagtgga tttgcgttct cgttccttct ttgaagaaat
    52981 ccaaaacatg ctgatcgaaa tcgaaaatac gaacttcatc gacacttcta tcttattttt
    53041 agatgcttca gacgaagaac tagtggctcg ctacaaagaa acaagacgag cacatccaat
    53101 ggcgatggac ggtttgatca ctgaagggat acgtaaagaa cgggcgatac tagacgactt
    53161 gaaagcccaa gcttccgtga tcattgatac gacaacattg acaccacgac aattacgtga
    53221 aaaaatcaat gagtcattca aatcaaaggg agaccaagga tttcggatcg aatttgtatc
    53281 atttggtttt aaatacgggt tgccgatcga ttcggatatt gtgatggatg tgcgtttttt
    53341 gcccaaccct cattatatcc cggaactaag accattgacc ggaaaagatc agccagtcta
    53401 tgattatgtg atgtcctttg atgaaacgca atcattctac cataaatttt tggatcttct
    53461 ggaaactgta ttgcctggat acattaaaga aggaaaaagc agcttgacag ttgcaatcgg
    53521 ctgtacaggc ggtcaacacc gttctgtggc attgactgaa cgtttggcaa atgctttagg
    53581 gaaaaactat aaagtcaatg tgacacatcg ggacaaggat aaaaggaaag agacggtgaa
    53641 ccggtcgtga aaatgaaaac ctaccggata agaaaaccaa agatcgtagt ggtcggcggt
    53701 ggcactggtc tgccagttat cctgaaaagt ttacgaaatc aaggagcaga tatcacagca
    53761 gtagtgactg ttgcagatga tggtggaagc agcggtgcaa tcagagaatc gatcgctatg
    53821 gctcctcctg gtgacttgag aaatgtctta gtggcgttat cggatatgcc acaattctat
    53881 gaagatattt tccaataccg tttcaaaaag gaagatcagt ttttagccaa tcatacgatc
    53941 ggtaatctga tcattgccgc agtttccgag atgcggggaa gcacttatga ggcgattcaa
    54001 ttattagcaa aaatgatgca tgtagatgga catgtttatc cttcttcaga aactcctttg
    54061 accttgcatg ctgtttttaa agacggaacg actgcagtag gagaatctaa aatcgcgatc
    54121 aaccgtaaaa ccattgaccg tgtgtatgtc tgcaacacaa atgataaaag tgaagccaaa
    54181 gcggctcgaa aagtcgtttc agcaattatg gacgccgaca tggtcgtttt gggacccgga
    54241 agtttattca ccagtatcct tccaaattta gtgataccag aaatcggcga agcaatgctc
    54301 aagacaactg ctgaaaccgt ctatatctgt aatatcatga cccaaaaagg ggaaacggaa
    54361 catttcacag atgccgatca tatacgtgtt ttacacaagc atttgaatca gccttttatc
    54421 gatacagtat tggtcaatac ggaaaaagtg cctgaagatt atatggaccc agagatctat
    54481 gatgagtatc tagtacaagt cgcacacgac ttcaacggat tgagaaatga aggatgtcga
    54541 gtgatttcca ccgacttttt agaattgaaa gatggtggtg tcttccacga tggagacaaa
    54601 gtagtagaag aattgttccg tttagttttc ggttcaaaat attaagcaat ttattgttgg
    54661 agaaaggagg agaataggat gtcatttgct gcagatgtga agaaagaact aactggtttg
    54721 gaagttcatc gagaacatgc gaaagcggaa ttagctgctt tgctgcgaat gaacggctct
    54781 ttaggtattg tgaaccagca gtttattttg aacgtccaaa cagaaaatgc tgcaattgcc
    54841 agacgtatct attctctttt gaaagatcat tatcaggtac gaagtgagct actcgttcgt
    54901 aaaaaaatga aattaaaaaa gaacaatgtc tatatcgtcc ggttgaagca aggcacaaaa
    54961 gatgtgttga tggatcttga tatcatggat ggtatgatgt tcaatggaca cgtttcggac
    55021 gagattatgg gaaattcgca aaaaatgcgt tcttacttac gtggtgcttt tatggcctca
    55081 ggttcagtca ataatccgga aacgagtcgt tatcatttgg aaatattttc tatttatgaa
    55141 cagcataacc aagacatttg cgatatgtta aattattacg gtctacatgc tcgaacgctt
    55201 gaacggcgca atggctatat ttcgtattta aaaggagcag aaaaaattgc tgatttctta
    55261 acattgattg gtgcaaccaa ctcaatgctc cgatttgaag atgttcgaat cgtacgggat
    55321 atgagaaatt cggtcaatcg tctagtcaat tgtgagacag caaatctgaa caagacgatt
    55381 gatgcggctt ctaaacaaat cgaaaacatc gaattgatcg aagcaaaggt cggtctacat
    55441 gcccttcctg aaaaacttca ggaaatcgca gaacttcggc ttcaacatcc agaggtcagc
    55501 ttgaaagaat taggggaaat gattccctca ggtgcaatat caaaatcggg aattaatcac
    55561 cgaattcgca aaataaatga atttgcggaa aaactgcgtg aagaagcagt ttaatccaaa
    55621 gaaaaaactg atacatgaag caggagatgc gaatgtatca gtttttttta aaagctatac
    55681 ttgcttttct tccgtcaaac tataattcca actttactgg agtaacataa tgttgaaagg
    55741 cttctattgt tttttaatac aatcgtataa aaagcgggga gacaactgaa tgaagatttt
    55801 agttttatac ataaactttt gcttaatata gaagtcctga tatagatagg atcaagtatt
    55861 tatctataac tggagattaa ttaaaaactg gaaggaactg ctcgatcaac cttgagaact
    55921 gtttttctta cttctggtca agattctcgt ggttggtgga taaggatggt tgataagttc
    55981 attggattag ctagtcgaaa acaaccaggt gtaattaatg tagaagtttt aacaagggga
    56041 tggagtgatt tattcagtga taatcataag ttcataaata gtaattggga tgtatggttg
    56101 gtatacgtta tacctgaact tgaactatca agatttggtt tagaatttga gaatcgatgt
    56161 ttataaaggg agggcaaaaa atgtgctttt ttacaacttt tactatcatt atgattgcag
    56221 tactttttat ttttctagat atagcaaagc gaaatacagc atttttatta tatcgggttt
    56281 tacttcgagc aggtctcata acatttatat cgatcgttgg attttttcta ttcacagtta
    56341 ttgtatttat ttggagaact acagcaccac cattgccaga gattacatat ggagaatttc
    56401 cgtttagtct tgaatatgaa atagatgagg aaagaatttt gattgaagat acggtgattg
    56461 cagaatttat aggaagtttt agagggaatg tgacaactca acctagacga agttggagta
    56521 taaaactttt aaataatagt ggagagttga aagcatttca ttatgaattc ttaataatag
    56581 aaagtgaaga tgtaacaatc actttaagta gtggctctgc tggatatttt atgaatgatg
    56641 gtggaagcaa gccacagctc acagtccgtg atcgtattgc aggttttatg catttttctc
    56701 actatgactt agaagaagct cgtaaaatac ttgcagaata tgggatcaca ttaatcaatt
    56761 gggaatatgc gccaccgata agaaattttt tcaatgatta ttattagtct tataaaaaac
    56821 agaatgtaaa atttatgtgc gcgagtattt agttttccat tttttgtttg atagatatat
    56881 agcattgttg aagcatggga tagtggtgcc aattttatca atgaggttgg tcaggcagtt
    56941 aacataattg gtgatgctat aaatccattt aattggaatt ggaggaatta ggtgtggaaa
    57001 cagtgagatt atggatattc atgaaaaatt ttcgttcggc tttgagatat gttattctgg
    57061 aatcaaaatc aggttattat ctgattgaca cagatcacta tactttttgg ggaggctacc
    57121 tttttccgat ttttaattgg ataatccccc ataaggttat gaaaattaac ttgcctgcag
    57181 aagaaataaa tagattaata ctcaacaaag atttacaaag gaaaagagag aaagaacatt
    57241 ctaatgttgc ttggataggt ccagcatttg cagtagtgac ttcttcaatt gtcggtccta
    57301 tgctagagag tatttatagt gatttttcta ttggaatacg ttatttattt gttactttaa
    57361 gtatagctgt tcttttgtgc ataaagggtt atgttagcaa gaagaataaa gatattttaa
    57421 cactaatcgg tgtaaacaat ttatctagta ctaaaattat actgtttcct gaatctataa
    57481 aagaaggatc aaggttgttg ataattttta taatgcttat actgttttct gttttatttg
    57541 gggtgatgtt tataga
//
LOCUS       DACDIG010000008        57059 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.008, whole genome shotgun sequence.
ACCESSION   DACDIG010000008 DACDIG010000000
VERSION     DACDIG010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.008"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            317..3151
                     /locus_tag="IV247_000557"
     CDS             317..3151
                     /locus_tag="IV247_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8776651.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            3342..3833
                     /locus_tag="IV247_000558"
     CDS             3342..3833
                     /locus_tag="IV247_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAP8776652.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            3936..4925
                     /gene="manX"
                     /locus_tag="IV247_000559"
     CDS             3936..4925
                     /gene="manX"
                     /locus_tag="IV247_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAP8776653.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            4958..5761
                     /locus_tag="IV247_000560"
     CDS             4958..5761
                     /locus_tag="IV247_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAP8776654.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            5782..6696
                     /locus_tag="IV247_000561"
     CDS             5782..6696
                     /locus_tag="IV247_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAP8776655.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            6779..7153
                     /locus_tag="IV247_000562"
     CDS             6779..7153
                     /locus_tag="IV247_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAP8776656.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            7292..7537
                     /locus_tag="IV247_000563"
     CDS             7292..7537
                     /locus_tag="IV247_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP8776657.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            7709..8773
                     /locus_tag="IV247_000564"
     CDS             7709..8773
                     /locus_tag="IV247_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP8776658.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            9019..9444
                     /locus_tag="IV247_000565"
     CDS             9019..9444
                     /locus_tag="IV247_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP8776659.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            9437..9886
                     /gene="nusB"
                     /locus_tag="IV247_000566"
     CDS             9437..9886
                     /gene="nusB"
                     /locus_tag="IV247_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAP8776660.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            9983..10837
                     /locus_tag="IV247_000567"
     CDS             9983..10837
                     /locus_tag="IV247_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAP8776661.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            10838..12175
                     /locus_tag="IV247_000568"
     CDS             10838..12175
                     /locus_tag="IV247_000568"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAP8776662.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            12180..12404
                     /locus_tag="IV247_000569"
     CDS             12180..12404
                     /locus_tag="IV247_000569"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAP8776663.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            12406..13296
                     /locus_tag="IV247_000570"
     CDS             12406..13296
                     /locus_tag="IV247_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP8776664.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            13305..14123
                     /locus_tag="IV247_000571"
     CDS             13305..14123
                     /locus_tag="IV247_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAP8776665.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            14230..14679
                     /locus_tag="IV247_000572"
     CDS             14230..14679
                     /locus_tag="IV247_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP8776666.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            14697..16379
                     /gene="recN"
                     /locus_tag="IV247_000573"
     CDS             14697..16379
                     /gene="recN"
                     /locus_tag="IV247_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAP8776667.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(16434..16550)
                     /locus_tag="IV247_000574"
     CDS             complement(16434..16550)
                     /locus_tag="IV247_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAP8776668.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(16623..17579)
                     /locus_tag="IV247_000575"
     CDS             complement(16623..17579)
                     /locus_tag="IV247_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP8776669.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            17750..18118
                     /locus_tag="IV247_000576"
     CDS             17750..18118
                     /locus_tag="IV247_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAP8776670.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            18288..18719
                     /gene="mraZ"
                     /locus_tag="IV247_000577"
     CDS             18288..18719
                     /gene="mraZ"
                     /locus_tag="IV247_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAP8776671.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            18740..19699
                     /gene="rsmH"
                     /locus_tag="IV247_000578"
     CDS             18740..19699
                     /gene="rsmH"
                     /locus_tag="IV247_000578"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAP8776672.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            19704..20105
                     /gene="ftsL"
                     /locus_tag="IV247_000579"
     CDS             19704..20105
                     /gene="ftsL"
                     /locus_tag="IV247_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAP8776673.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            20106..22292
                     /locus_tag="IV247_000580"
     CDS             20106..22292
                     /locus_tag="IV247_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP8776674.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPSED
                     PDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYRNL
                     EADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVTGW
                     SKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            22318..23280
                     /locus_tag="IV247_000581"
     CDS             22318..23280
                     /locus_tag="IV247_000581"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAP8776675.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            23294..24655
                     /locus_tag="IV247_000582"
     CDS             23294..24655
                     /locus_tag="IV247_000582"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAP8776676.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVSECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            24681..25769
                     /gene="murG"
                     /locus_tag="IV247_000583"
     CDS             24681..25769
                     /gene="murG"
                     /locus_tag="IV247_000583"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAP8776677.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            25784..27004
                     /locus_tag="IV247_000584"
     CDS             25784..27004
                     /locus_tag="IV247_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAP8776678.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            27160..28485
                     /gene="ftsA"
                     /locus_tag="IV247_000585"
     CDS             27160..28485
                     /gene="ftsA"
                     /locus_tag="IV247_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAP8776679.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            28508..29749
                     /gene="ftsZ"
                     /locus_tag="IV247_000586"
     CDS             28508..29749
                     /gene="ftsZ"
                     /locus_tag="IV247_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAP8776680.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            29766..30443
                     /locus_tag="IV247_000587"
     CDS             29766..30443
                     /locus_tag="IV247_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAP8776681.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            30457..31056
                     /locus_tag="IV247_000588"
     CDS             30457..31056
                     /locus_tag="IV247_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAP8776682.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            31071..31358
                     /locus_tag="IV247_000589"
     CDS             31071..31358
                     /locus_tag="IV247_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAP8776683.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            31407..32189
                     /locus_tag="IV247_000590"
     CDS             31407..32189
                     /locus_tag="IV247_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAP8776684.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            32210..32917
                     /locus_tag="IV247_000591"
     CDS             32210..32917
                     /locus_tag="IV247_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAP8776685.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            33179..35977
                     /gene="ileS"
                     /locus_tag="IV247_000592"
     CDS             33179..35977
                     /gene="ileS"
                     /locus_tag="IV247_000592"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAP8776686.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKSYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(36026..37552)
                     /locus_tag="IV247_000593"
     CDS             complement(36026..37552)
                     /locus_tag="IV247_000593"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAP8776687.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(37567..38214)
                     /locus_tag="IV247_000594"
     CDS             complement(37567..38214)
                     /locus_tag="IV247_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAP8776688.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(38398..38727)
                     /locus_tag="IV247_000595"
     CDS             complement(38398..38727)
                     /locus_tag="IV247_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAP8776689.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            38904..39632
                     /gene="sfsA"
                     /locus_tag="IV247_000596"
     CDS             38904..39632
                     /gene="sfsA"
                     /locus_tag="IV247_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAP8776690.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            39648..40661
                     /locus_tag="IV247_000597"
     CDS             39648..40661
                     /locus_tag="IV247_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8776691.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            40661..41938
                     /locus_tag="IV247_000598"
     CDS             40661..41938
                     /locus_tag="IV247_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP8776692.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            42001..44703
                     /locus_tag="IV247_000599"
     CDS             42001..44703
                     /locus_tag="IV247_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8776693.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            44855..45172
                     /locus_tag="IV247_000600"
     CDS             44855..45172
                     /locus_tag="IV247_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8776694.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            45202..45522
                     /locus_tag="IV247_000601"
     CDS             45202..45522
                     /locus_tag="IV247_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP8776695.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            45630..47090
                     /locus_tag="IV247_000602"
     CDS             45630..47090
                     /locus_tag="IV247_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8776696.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            47158..47379
                     /locus_tag="IV247_000603"
     CDS             47158..47379
                     /locus_tag="IV247_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8776697.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            47410..47592
                     /locus_tag="IV247_000604"
     CDS             47410..47592
                     /locus_tag="IV247_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAP8776698.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            47592..48005
                     /locus_tag="IV247_000605"
     CDS             47592..48005
                     /locus_tag="IV247_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAP8776699.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            48128..49309
                     /locus_tag="IV247_000606"
     CDS             48128..49309
                     /locus_tag="IV247_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8776700.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            49698..49771
                     /locus_tag="IV247_000607"
     tRNA            49698..49771
                     /locus_tag="IV247_000607"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:49732..49734,aa:Arg,seq:cct)
     gene            complement(49840..50979)
                     /locus_tag="IV247_000608"
     CDS             complement(49840..50979)
                     /locus_tag="IV247_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8776701.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEVK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(51093..51677)
                     /locus_tag="IV247_000609"
     CDS             complement(51093..51677)
                     /locus_tag="IV247_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HAP8776702.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            complement(51804..52235)
                     /locus_tag="IV247_000610"
     CDS             complement(51804..52235)
                     /locus_tag="IV247_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP8776703.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(52253..52573)
                     /locus_tag="IV247_000611"
     CDS             complement(52253..52573)
                     /locus_tag="IV247_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8776704.1"
                     /translation="MITFERIKELAKKQGKSLNKVEEDLGYGKNVLYRLKNSNPSTER
                     LQEIADYFDVSVDYLLGREEKETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            53305..53562
                     /locus_tag="IV247_000612"
     CDS             53305..53562
                     /locus_tag="IV247_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HAP8776705.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(53533..53841)
                     /locus_tag="IV247_000613"
     CDS             complement(53533..53841)
                     /locus_tag="IV247_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8776706.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            53919..54692
                     /locus_tag="IV247_000614"
     CDS             53919..54692
                     /locus_tag="IV247_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP8776707.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIRK
                     HGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSST
                     DSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPKA
                     DGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            54707..54910
                     /locus_tag="IV247_000615"
     CDS             54707..54910
                     /locus_tag="IV247_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8776708.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            54926..55246
                     /locus_tag="IV247_000616"
     CDS             54926..55246
                     /locus_tag="IV247_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAP8776709.1"
                     /translation="MQYLEAKIPVPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRR"
     gene            complement(55428..56210)
                     /locus_tag="IV247_000617"
     CDS             complement(55428..56210)
                     /locus_tag="IV247_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8776710.1"
                     /translation="MDISDTPYYQKLIASSKLIGETIAPSLDVVNAMQPAIEQAKKLI
                     AIQDTFIASQAILNDSLIQIVEKRNKSIANMIPPSLFAIQKQLESPSFNIAKKMAATM
                     PKPYFSDISKITERFAVDMSKYDFRIGVATQELEKAFSSEPEVSTKNLLFKDTSNPSD
                     EFEAKFFDILNEQRKVLDDSIKLFEEINTAKNKDITNEPSEKQQASPAFYKDKMWYLE
                     QTGAALIGLVVTEIVNITIGVDPNNTISLALFLRYLLQFLIN"
     gene            56261..56968
                     /locus_tag="IV247_000618"
     CDS             56261..56968
                     /locus_tag="IV247_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP8776711.1"
                     /translation="MQELVILKNKEAVTTSLQVAETFRKEHRAVLKAIDDLKDGLAQK
                     YADLFWEDVYIHPQNKQAYRIVYMNRDGFTLAVLGFNNTQKVLEFKLKYIEAFNEMEK
                     AIRKNTAFDTIEDMMIYQLEEMKDVKKDVSMLKNTMRISGQQEFEIKQKGNTKVMEVL
                     GGKESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRM
                     EIDQLNRQQRLFGDENE"
BASE COUNT        19033 a         9622 c        11991 g        16413 t
ORIGIN      
        1 tttactttgt ctttttacac ttaatatttt tttgccaatt tggaatcagg atgttttgat
       61 aactatcgtt ttcaattgat ggaagctata ttgtttgcta tcagtgaaat aattatatgg
      121 taaacagtgt tctttttctt gtttccatta taaaaatcgt atcgggattt ttcgttcttt
      181 taaattgaac atttctatct atccgcgtgt tatcaacaaa acaaaatcag gtaaatagtt
      241 gacgaaacag aaataaatac atagtattat gttttgaagc ctaagtacaa tagtctatag
      301 aaaaggagcg ataccattgg caaaacgcat tgaccgaatt tatatgtatg tcaaagaaaa
      361 aaccgctcat ttaacaaagg cagaatatga tcaaggagta acaacacagg aaattgctga
      421 agtgttaggg attcagcgaa caaatagcag taaagatctg aatcagctag tgcgcgaagg
      481 aaaactgata aagacagacg gacgaccagt acgttacata tatcaaacac tcgttacgca
      541 cgtaaagcca gttacaaaac atgtagtcag ttacaaagaa acgccggtct ctgaagaaaa
      601 aaatacactc caaatagata ctaaggatat ctttgcaaaa atcattggag ctaacggcag
      661 catgaaaaat tcagtggaac aggcaaaagc tgcgattttg tatccgccaa gaggattaaa
      721 ttgtttgatt acaggaccta ccggatcagg aaagacttat tttgcacacg cgatgtatca
      781 ttttgcaaaa gcgcatcatg taatagatga ggaaacggaa ctgattgttt tcaattgtgc
      841 tgattatgcc aataaccctg agcttttgat gagtcattta tttgggtacg caaaaggtgc
      901 ttttacaggc gcagatgaag aaaaaatggg gattatcgat caagcggatg gcggtatgct
      961 gtttttggat gagattcacc ggttgccgcc tgaaggtcaa gaaatgatct tttactttat
     1021 ggatcatgga acttacagtc gtcttgggga aacaacgaaa tcccatgaag caaatgtacg
     1081 gatcgtaggt gcgaccacag aagacccagg ttcttctcta ttagaaactt ttgttcggcg
     1141 tatcccaatc aatatcaaac ttccggcttt tgaaaaacgt cctgcaaatg agaaagtcga
     1201 tttagtaaaa atcatgattg cccacgaagc aaaccgcacg cagcgaaaga tttctttgac
     1261 agaagatgta gcaaaagcat tgatcggcag tgtgacctat ggaaatatag gacaattgaa
     1321 gtcaaatatc cagttagtat gtgcacgagg atttctaaat catatgcaat cacccgaaat
     1381 ctcgatcaca atcgatgatt tgacagaagg gattcgtagc ggattgattc aattagcaag
     1441 caaccgcagt gcaatggcag agctttctaa aatattggag ccaaagataa ctgtagcacc
     1501 aaatgatacg atcatgaaaa tccaatcgga ctcctatgag ttaccttata atctatatga
     1561 tatcatcgga gataaagcag ctttactgaa gtcggatggt ttggatcaag aagcaatcaa
     1621 tcatttcatt tctacagata tcaacatcca tctgaagtct ttctataaag accatggttt
     1681 ttcttttaat gcagacagca aattagcaga atttgtagat ccgaaaatca tagaagtgac
     1741 caatcagatt tatgtaatgg tcaaaagatc tttaccatat gagtttcagc aaaactttat
     1801 ttatgctatg agcttgcata tcagttcttt tttgaaaaaa atcaacttag gagaagaacg
     1861 gcatacgaat gataacatcc gtgagatggc ttttgatttt ccagatgaat atgctgttgc
     1921 gaaagaagtg cgtagttata tcgaaacgta ttttcaagtg gagattccag aaagtgaaga
     1981 ctattacttg accgttctac tcgtctcgtt acgggcaaat caagcaagcg gcagaatcgg
     2041 tgtagtcgtt gcagctcacg gaaacagcac agcaagcagt atggtccaag ttgtccaaca
     2101 gttgttagat gcagagaacg tacgagcagt agacatgcct ttggatatgg atccgaaaac
     2161 agcattggac cgaattgaac gcaatgtaca agaagtagat gaaggaagcg gggtcatact
     2221 cttagtagat atggggtccc ttgcgtcgtt taattcgcaa attcaaagag acactggaat
     2281 tccagtacgt actgttgata tggtaacaac ttctctagtt ttggaaactg tacgaaaggt
     2341 ctcagtatta gggacagatc tagatatgtt gcatgaatca ttgaaaaatt ttcgaggtta
     2401 cgcagaaata tcttctgtag aatctgttca ggaagcaaaa cctattcata agaaaaaagc
     2461 tgttttggct atctgcgcat ctggcgaagg tacagcacaa agaatcaaag aattgatcga
     2521 acgagcggta aacaagcgtc aagaaacaga attagaagtt ctagctttgt cgattgtgga
     2581 agtgaaagaa gaactgccag ttatccaaaa agaatatcag atcatagcta ctactgggat
     2641 tactgatttg aatatccaag ccccatttat tcctttagaa cgttttattg atcaaaatat
     2701 cgaagtcatt ttggatcagt tgctgatgga atcagaattg gaggaaactg aattcatctc
     2761 cctagatgaa gagtcagcta aaaatacgtg tgttgagttt atctctgata acttcatctt
     2821 tattaatggt agtaagttaa tcgatccaat gtggcaattt tctacacaaa tctctcagac
     2881 gacgggaatc ggcgatgaag aatatggatt caaaatcaac ctagtgatgc acacagcagg
     2941 aatgatcgaa cgtattattc ggaatgaacc attgactgtg gaagaaaacg aattgacaaa
     3001 tacaacgaat gatcctcttt atagccaatt ggcagcctca gttgttctgt tggaagatca
     3061 gataaaagta aaagtgccga tagaagaaat gtattatctg ctacgtctag tccataatca
     3121 gttagataaa aaagaataca ctgtgccatg aatacacaaa aacgatacac ttttatagtg
     3181 tatcgttttt gtgtattggt taagaatcct ttaaattcaa ttagtgtatc gtaaaaaagt
     3241 tggcacgctt tttgcttata gtagtgtgtg ctaggaggtg gttgttcaca cagaaatgat
     3301 aggggtttct gtgaagtagt gaggaagaag gagttttgca gatggatatt cgattggcac
     3361 gtatagatga tcgtttaatc cacgggcaag tcgcaacagt ttggtcgaag caaacaggtg
     3421 ttgaacgtat cattgtgatc agcgatgtag tcgctcaaga taaattgaga aagtttctac
     3481 taaaagaagc tgcaccgccg gggatcacag caaatgtaat aaccgtgcag aaaatgattc
     3541 aaatctatca tagtggatta ttatcacgag caaaagtgat gcttttattc accaatcccc
     3601 aagatgttga agcggttgtc cgctgcggtg tacagttgaa gtcgttgaat atcggcggta
     3661 tgagttttac tgaaggcaaa tcaatgatta ccaattttgt ttcagtaaat caacaagata
     3721 ttgattcctt tcgttacttg aacagtcaag aagtggagtt agagattcga aaagtacctg
     3781 ctgacaggaa aatccttttg atggaactac tagcgaaaat caaaaaggtg tagcaagaat
     3841 ggttgttgga aaaggtcaaa taattctgtt gtaatggtta gcaattatta atatttttga
     3901 atttgttaca acaaaagtaa ataggaggta taaatatggt agggattatc ctagcaagtc
     3961 atggacaatt tgctgaagga atcttacaat ccggttcaat gatctttggc gaacaagaaa
     4021 acgtcaaagc cgtgattttg aaaccaagtg aaggtccaga tgacttgaga gccaaattgg
     4081 aagaagcggt tgcttctttt gataatcaag acgaagtgtt attcttagtt gatttatggg
     4141 gcggaacacc gttcaatcaa tcaaacacat tattcgaaga gcacaaagat aaatgggcaa
     4201 tcgtcagcgg tttgaattta ccaatgttga ttgaagccta cgcatcacgc ttttcaatgg
     4261 aatctgctca tgaaattgca gcacacatca ttgaaacagc aaaagacggt gtaaaagtaa
     4321 aaccggaaga attagaaccg gcagaagcac cgaaagcagc agttgaagac gcacaaccta
     4381 aaggagcact tccagaagga actgtggtcg gagacggaaa aatcaaatat gtattagcac
     4441 gtgttgactc acgtctgtta catggacaag ttgcgacagc ttggacaaaa gcagtgcaac
     4501 caaaccgtat tatcgttgtt tcagatgcag tatcaaagga tgatctgcgt aagagactaa
     4561 tcgaacaagc tgctcctcca ggtgtaaaag caaatgttat tcctattagc aaaatgatcg
     4621 aagtagcaaa agaccctcgt tttggaaata ccaaagcatt gcttttattt gaaaatcctg
     4681 aagacgtatt gacagctgtt gaaggtggcg tagacatcaa agaattaaat gttgggtcaa
     4741 tggcgcattc ggtcggtaaa gtcgttgtaa gtaaagtatt gtctatggga caagaagacg
     4801 ttgaagcgtt tgagaagctt gaacaaaaag gtgtgaaatt cgatgtacgt aaagtaccta
     4861 atgattcacg cgataacatg gatgatattt tgaaaaaagc aaaagctgaa ttagcaaaag
     4921 cgtaataaat aatattaaaa tttataggag gcttatcatg tctattattt caataatttt
     4981 agtcgtatta gttgcctttc tagctggtat ggaaggtatt cttgatgaat ttcaatttca
     5041 tcaaccacta gtggcatgta cattgatcgg tttagttacc ggtaacctcg aagctggtat
     5101 cgtacttggc ggtacgcttc agatgatcgc tttaggttgg gcaaatatcg gtgccgctgt
     5161 agcaccagat gctgcattag catcagtagc atcagcaatt attttagttt taggtggaca
     5221 aggtgttaaa ggtgttcctt ctgcgatcgc gattgccgtt cctttggctg ttgcagggtt
     5281 gttcttaaca atgatcgtac gtacaatcgc tgttccaatc gttcatttga tggatgcggc
     5341 tgctgaagaa ggaaacatcc gtaaggttga aatgtggcac atcattgctg tatgtttaca
     5401 aggggttcgt atcgcggtcc cagcagcagc gttgctattt atcccagcac aaactgtgca
     5461 atcattcctt gaatcaatgc cagcatggtt aacagatggt atggctatcg gtgggggaat
     5521 ggtcgtagct gtaggttacg ctttggtaat caacatgatg gctacaaaag aagtatggcc
     5581 attcttcgtt attggtttcg ttgtagctgc aatttctcaa ttaacattga ttgctctagg
     5641 tgctttaggt gttgcattag ctcttattta cttgaacctt tctaaaatgg gtggttcatc
     5701 aaataatgga ggcggaagca actctggtga ccctttaggc gacattttga atgactatta
     5761 atcttgaagg aggaaaataa aatggcagaa gaaaaaatca aattatcaaa aaaagatcgt
     5821 ttagctgttg cgtggcgctc taccttcatt caaggttcat ggaactacga acgtatgcaa
     5881 aacggtggct gggcattttc tatgattcca gcaatcaaaa aattatacaa aacaaaagaa
     5941 gaacgttctg cagcattaaa acgtcacttg gaattcttta atacacaccc atacattgct
     6001 tctccaattt taggggttac attggctctg gaagaagaac gtgcgaatgg cgcgcctgtt
     6061 gatgatgtag cgattcaagg ggttaaagta ggtatgatgg gtcctttagc tggtgttggt
     6121 gacccagtgt tctggtttac agtacgtcca atgctaggtg cactaggtgc ttcattagct
     6181 atgggaggaa atatccttgg accaatcatc ttcttcttag gttggaacat tattcgttgg
     6241 gcattcatgt ggtatacaca agaatttggc tacaaagctg gttctaaaat tactgatgac
     6301 ctttctggcg gattgttaca agacgtaaca aaaggtgctt caattttagg gatgttcgta
     6361 ttagctgccc ttgtacaaag atgggtatca atcaaattct taccagttgt atcaactgta
     6421 aaattagata aaggtgctta catcgaatgg gataaattgc cttcaggtgg tgaaggaatc
     6481 aagagtgcat ttgaacaagt taataatggc ttagcccttt ctcctgaaaa ggtaacaaca
     6541 ttacaagaca acttagacca attgattcct ggtttagctg cattaggatt aacattgtta
     6601 tgtatgtggt tgcttaagaa aaaggtttct ccaatcgtta tcatccttgg tttattcgta
     6661 gtcggtgttg ttggacacgt catcggactt ttataatcca taaaaatata gctcgggcgt
     6721 tgtcccgagc tttttttata tagtatactg ttcataaaca aatatctgga gggagaaaat
     6781 ggttcaatca atcaatacaa aagtagactt ggtaattgat gctacttcgt ttacgggtct
     6841 gacagattac gggcaaatca tgataggtga taatggtttt gagttttacc atgcgcgaga
     6901 tagacgcaaa tatatccaaa ttccatggga agaagtggat tatgtactag catcagtcat
     6961 gctaaaggga aaatggattc ctcgatatgc cattcaaacg aaaaaaaatg gtacatacac
     7021 attttcttcc aaagaaccta aaaaagtgct tcgtgcgatc agagaatatg tagatgaaga
     7081 tagaatggta caatcattga gtttctttga tgtagtgaaa cgtggtattc gtagtatgtt
     7141 taaacgcaag taaaaaataa agaaaacttc taagaggagg ttttctttat ttttttactt
     7201 tgcagaagaa aatatgaaat agcaaaacca attatttgtc ccctttaaat cccatatgct
     7261 aaaattgatt agcaaagaag gaggttatat tatgagttgg ttatggacat tgattgttgg
     7321 agccgtgatc ggagcaattg caggtgcgat caccagtaaa ggaaattcga tgggctgtgt
     7381 aacgaatatc attgctggac tagtcggttc aagtattgga caagcgattt tcggcgaatg
     7441 gggaccaaaa gctgcaggta tggcacttat tccatctatt ttaggcgcag tgattttggt
     7501 agcagttgtc tcatttttct ttggaaaacg atcataagtt tttacaaaat agtaaactaa
     7561 aaggagttgc agcgagactg tgactccttt ttggctttat ttatttaaaa taaaaagaag
     7621 ttttccggaa atttttattt aactgtcgtg tacgaagaaa agtttgctat aatggaaaaa
     7681 agcgtgcaaa gaaagaggga gtttcattat gatgctacgt gtagaaaaat tgcgcaaaaa
     7741 aatgcaagaa gaaaatttgg attcattttt ggttaccagt ccgtacaatc ttcgttactt
     7801 aacgaacttt acaggtacga caggattagc tgttattact ttggaaaaag cattttttat
     7861 tactgatttt cgttataccg agcaggcggc agcgcaggct caagggtttg aaattattaa
     7921 aaatgtcgga cctatttttg atgaggtagc aaacttagta cgcaaagaag aactgcactc
     7981 attagctttt gaggaaacaa cagtctcatt tttagaatat tcggttttag aagaaatcat
     8041 cgatgcggaa ttagtacctg tttcacagat gatcgaagaa ctcagagaag tgaaagatga
     8101 agaagaaatc gctattatcg aaaaagcatg tcatattgcg gatatggcat atgatcacat
     8161 cctgaaaatg atccaaccgg gcatgactga gattgaagtc gcaaatcaac tagactttta
     8221 tatgcgttct cttggcgctt caggtgtttc ttttgatacg atcgttgcta gcggattgcg
     8281 ttcggctatg ccgcatggtg tagcaagcaa aaaaatcatc gaacaaggtg acttgatcac
     8341 attggacttt ggttgctatt acgaagggta cgtttccgat atgacacgta cattcgctat
     8401 tggtgatcct ggggaaaaat taaaagaaat ttatcaaata gtcttagatg ctcaattagc
     8461 agttattgat gctgcaaaac caggaatgac tggtgtccaa ttagatgcag ttgctagaga
     8521 ttatatttca aaacatggtt acggggaagc atttggacat tctacaggac atgggatcgg
     8581 tttagagatc cacgaaggac caaacgtttc ttctcgtgca gaaaagcaat ttgtaccagg
     8641 aaatgtgatc actgatgaac cagggatcta cttaccagga atcggtggag tacgtattga
     8701 ggatgacttg ttgatcacag aagatggaaa tcgtgtgttg actcgttcac caaaagaatt
     8761 gatcatctta tagtttcatc aatagaagaa gtaagcgtaa aaatcagtgt tcacagactt
     8821 tgttatagaa aatcactcga tttaatgata cactatagaa gaacttttaa gctgtaagag
     8881 ataataaagt tgggtgtttt ctccaattca tagcagtttg cagctgatcg gcggcagttt
     8941 tcttaccacg tgatcactat gccgcatcat agagaattcg gatgtatctc gtgcttaggc
     9001 aaagggagga ataaataaat ggcagatgaa aaaaatttga ttttaaacgc aaaccaagat
     9061 ttaggagaga tcgtgatcgc tcctgaagtc atcgaagtaa ttatcgggat tgcagcttct
     9121 aaagtagaag gcgtatacgg aatgcgcgga acttttgcca gcaatgtcac tgaattatta
     9181 ggacgtgcag ctcacggaaa aggtgtttac ttagcaaacg aggaagatgg cttgaaagta
     9241 gatctttact gttatctaga atatggtgtt tcagttccta aagtagcaat ggaaatgcag
     9301 gaacgggtca gccagcaagt ccttttcatg acggatatcg atttatctga agtcaatatc
     9361 catgtagtag ctgttgttcc tgaaaaatta gaacaaccaa caatcgaaga actttttgaa
     9421 gacgaagagg aagagaatga ataaagaatt atcaagacac gagattcggg aaatggcatt
     9481 gcaggcttta tttccattag acttcaatgc agacctaaca aaagaagatg cgattttcaa
     9541 cgcaatcgaa ttagaccacc gcgacatgat caacgaagat gaatcagagt ttgttcctgt
     9601 ttatcttgat acgctagttg gcggtgtctg tgcaaaaaaa gatgaattgg ataaagtgat
     9661 tgaaaaacat ttaaaaaaca attggcacat caatcggatc tcaaaaatgg atttggtgat
     9721 cttgcgtatc gcgatttttg aaatgactta tgtgacagat gtaccagcgg ctgttgcttt
     9781 aaatgaagca atcgagttgg caaaaacatt tagtgatgac cgttcacgta aattcgtcaa
     9841 cggtgtttta tccaatgtat tgaaagaatt agaagctggg gcgtaagtcc tagctttttt
     9901 ctatgaatca tcataaacct gttataaatc gggaaaattc tatggtagaa tgaaaacaca
     9961 aaaaatatct gggagtggtt agatggcaga attgattaac ggaaaagaat tagctgaaaa
    10021 aatgcaggca gagattgctg aaaaaatcca aaaattgaaa gataatggta tccatcccgg
    10081 attagtcgtt ttattggtag gggaaaatcc agccagccaa atctatgtaa gaaataaaga
    10141 acgtgtagca aaagaaatcg gaatccattc attggtagag cgttatcctg agacgatttc
    10201 tgaagacgaa ctacttgcag aaattgaaaa atacaatcaa gatcctagtt ttcatggtat
    10261 attggtccaa ttaccattgc caaagcatat agatgaagaa aagatcctgt tagctatccg
    10321 ccctgaaaaa gacgtagacg gttttcatcc gatgaattta ggcagactcc ttgcgggtaa
    10381 accggacaag attccttgta ctccatacgg catcatgaaa atgtttgaag cttattctat
    10441 cgatccggcg ggtaaacgag cagtagttat cggacgaagc aatatcgtcg gtaaaccgat
    10501 ggctcagcta ctcttgatgg cagatgcaac tgttactgtt gctcattcta aaaccgaaaa
    10561 tctggctgaa ctggcacaag aagcagatat tttagtcgta gcaataggac gtggacattt
    10621 tgtcacaaaa gaattcgtaa aaccaggtgc tgtagtgata gatgtaggga tgaatcgtga
    10681 tgctaacggt aagctgatcg gtgatgtgaa atttgatgaa gtggagccta ttgcaggctg
    10741 gatcactcct gtgccaaagg gtgtagggcc aatgactatc acgatgctga tgtatcaaac
    10801 cgtaaaatct gccgaagctt ttggagcagg tgaatagatg gcacaagaat atttaactgt
    10861 cactacatta acgaagtatt tgaaaagaaa atttgatgcc gatccttatt tagaacgcgt
    10921 atatttaaca ggcgaaatat cgaatttccg tttacgtgcc aatgcccatc aatatttcag
    10981 cttaaaagat gaccatgcaa aaatatctgc tattatgttc aaaggagcat tccaaaaatt
    11041 acgattccag ccaaaagaag gaatgaaagt ccttgtcgtt gggcgtattt cattatatga
    11101 agcgggaggt tcttatcaaa tctatgtaga gcacatggag cctgatggaa taggtgcttt
    11161 atatcaagaa ttggaagaac gaaaagaaaa gctgatgaag gaaggccttt ttcaagggcc
    11221 taaaaaaatg ctgccacgtt tcccaaagcg aatcgcggtc cttaccagtc caagcggagc
    11281 ggttatccgc gatatcatca cgacggtgaa aagaagatat ccaattgtcc aattggtttt
    11341 gttcccaaca gttgtacaag gaaaccaagc agcggacaat gttgtcagga atatccagag
    11401 agtagaagag atgggcaatt tcgatacaat gatcatcggt cgtgggggtg gttcgattga
    11461 ggatttatgg ccgtttaatg aagagcgggt tgctcgggcg atttatcaag ctaatacgcc
    11521 ggtgatttca tctgtaggac atgaaacaga taccacgatc gctgatttag tagcagatgt
    11581 tcgggctgcc actccaacag cagctgcaga attagctgtt cctgttttgt cagaagagtt
    11641 gatgcgcatt caagaacggc aagctagatt gcagcaggcg tttgtacgcc agatccagcg
    11701 caaacaagaa cggttcgagc gtgctcgacg ttcctatgta tttcggcagc ctgaaagatt
    11761 gtatgaaggt cagaccatca aattagacca gttgaaacag cgattatatc aagctacaca
    11821 gcaaatttat catgcgaaag aaaaacaaac gatcgcatta agccaccaat tggaacaagt
    11881 ggctccgctt tatcgagtta aagcagctcg ccaagaaact gcttatctag aaaaacgctt
    11941 agaagagaaa atccaacaat atatgaaaca acagcagcag cgttttgctc aagctgttca
    12001 atctcttgat ttgttgagtc cattgaaaat catgggaaga ggctacagct atacgacgtt
    12061 aaatgatcaa gtagtaaaaa gtgtcaccga gattcatcaa ggggatttgc tgacgatcca
    12121 ttatcaagat ggaatcgtta aaggacaagt acaagagata gacaaaggag aataaaaata
    12181 tggaaaatcc aacttttgaa gagtcactgc aagaattaga aaaaattgtc atgcagttag
    12241 aacaaggaga cgttccatta gaatcagctc tagattcgtt taaacgagga atggaattaa
    12301 gcaaacattg ccaagataca ttagctaaag cagaagagac cttgacgaaa atgatgacag
    12361 aatcaaatga agaagttgat tttgacggaa acgaggaaag ctgagatgag attaactgac
    12421 ttttcagcta ttcatctacc tgctgtagaa aaagaaatct tgtctttttt agatgagcat
    12481 acaacggata gaggactcta tgatgcgatg agttattcag tgaaagcagg cgggaaaaga
    12541 atacgtccct tattactttt aacggccgtt gcttcctttg atgagcctat tgatgttccc
    12601 gtctatcaag tagcagctgc tttagaaatg gtgcatacgt attccttgat ccacgatgat
    12661 ttgcctgcga tggacaatga tgatcttcgt agaggaaaac ctaccaatca taaagtattc
    12721 ggcgaagcat tagctatatt ggccggagat ggtttgctta caggagcatt ccagctaatc
    12781 agtatggcac acttaggtaa ttctcctaag cttctgctgc ttcagcaatt agctgtttgt
    12841 gcgggatcac aaggtatggt tgctgggcaa gctgcagata tcgagggcga atcgaaaaag
    12901 ctttctttag aagaattagc atttattcat gaaagaaaga cagggcattt gattcgttat
    12961 gccttgctgg ctggtggcat attagcgaag caacctgaag aaatcttatt gctacttcaa
    13021 cgattagccg aacatttagg tttagctttt cagataagag atgatctact cgatgtgatt
    13081 ggtacaacga aaacattagg gaaaacagct ggtaaagatg aacgtatgga aaaaaatact
    13141 tacccgcgtt tattaggcct agaaaagaca cgtgaggcgt tggagattga gctgttatcc
    13201 gccaacaaga tcatagacaa attagaagag gacgtttttt cttttgatgg cgggctgatg
    13261 cgccagatga tcaagcaatt tgatgtggag aaatgaggga aagaatggaa aaagaacgcg
    13321 tagatgtatt agcggtcaaa caaggattat ttgaaacaag agaacaagcc aaacgttcag
    13381 tgatggctgg tttgatttac aacgaaaaaa acgagcggtt tgataaaccg ggagaaaaaa
    13441 ttccagtgag tagtgaatta agagtcaaag gtaaaaaact tccttatgtt tctcgagggg
    13501 gattaaaatt agaaaaagca ctcaaagcat ttgatctatc tgttgaagga aaaacattgt
    13561 tagatattgg tgcatccact ggcggattta ctgatgcagc tttgcaaaat ggcgcaaaaa
    13621 tgagttatgc tttagacgtt ggctacaatc agctagcttg gaaaataaga caagatccta
    13681 gagtcgtcgt aatggaaaga gtgaattttc gttatgcaaa acccgaagac tttaccttag
    13741 gaattccaga agttgctgtt atcgatgttt cttttatttc actaaaactg atgctgcctc
    13801 cattacatgc tattttaaaa gaagagggag aagtgattgc tttaatcaaa ccccaattcg
    13861 aagcaggcag agaagctgta gggaaaaacg gaattgttcg tgaccctcag acccatcaaa
    13921 aagttttaga agacattttg tcatttgcgg cacaaggtgg atatgatgtg ctggaattaa
    13981 gttattctcc gatcacgggg ggagaaggaa atatcgagtt cttggctcac ttgaggaaag
    14041 tacccgaaag cggaacaatc aattcagcta ttaatatggc agaagtggtt tcaaatgcac
    14101 acgaacagtt tgaccataaa taaattgtaa ctgtgaaaat caagtgagaa gtatgcggat
    14161 tcttctttat tataccaagg aaataagcta tcattgatta agaagaatat gctaaggagc
    14221 gaagacgtaa tgagaaaaaa agatcggcat cgtttgatta ctcgattgtt gagtgagcaa
    14281 gatatccgaa agcaggaaga atttgttgaa atcctgaaaa gcaaaggaat atctgttact
    14341 caagctacga tttccagaga tataaaggaa ctgaaactga tcaaggtacc ggcttcagat
    14401 ggcggctacc gttatagcat accagctgaa acaaacgaag atgtaaatac taaattggaa
    14461 aaactattaa aagacgcttt cgtggcagtt gaccaaatgg aaaaatttgt tattttgaaa
    14521 acattgccgg gaaatgcttc ggcagcagca aatttgattg ataaacgtta caagaaagaa
    14581 ttattttcga ttatcaatga tgacgataat gttttgatga ttacacgaac tgaagaccag
    14641 gcagttttat tgaagaaaga cttcctgcat tacctttaag ttgaggagcg aatgtgatgc
    14701 tacaagaaat cagtattact aactttgcga ttattccgga attgcggctt tcttttcacg
    14761 aaggaatgac cgcgctgacc ggagagactg gtgcgggtaa atccattatt atcgatgctt
    14821 taggtctgtt agcaggagga agaggttcaa gcgattatat acgtcaagga gcagaaaaat
    14881 gtgtgcttga agggttgttt gaactaccaa aacaagaagg attctctgaa ctgatggttg
    14941 aattagggat cgaaacagat gaggacaatc tgattgttcg acgggatatg tccttaactg
    15001 gaaaaaatgt ttgtcgagtg aatggtcata ttatcacatt agctaatctg agaagaatcg
    15061 gcagttattt agtagatatc caaggacaaa acgaacatca ggaactgctc cagcctgaat
    15121 cacatcttgc tttattggat cgttttggag atgtagcttt ccaacagaag aaaaagagtt
    15181 atcagcaaga atatatctct tatcgtgagc tggaaaaaag ggtacgaaaa gtgcagcaaa
    15241 atgaaaaatc ctatgttcag agaatagata tgctccattt ccagcaagaa gaaatagctg
    15301 ctgctgattt gcaagtcgga gaagaagaga aattaaaaga agaaagagaa aaacttagca
    15361 attatcaaaa aattgctgat ggattagctg caggttacgg agcactgacg aataatgaac
    15421 aaaacagtgt agatggtgtt ggcttggccg tttcagaact gcaagggatc gcccatctag
    15481 atgtggagta tgaagcaatt tatgaaaata tccaaagtgc ttactatttg ctgcaagatg
    15541 caatcggcga tatgagtcgt cagattgatt tattggagtt agatgagagt cgtttagaag
    15601 aagttacgca acgtttagaa ttaatccgac agttgaaacg taaatacggc gaatccattg
    15661 agtcaatatt ggcttactat gacgaaatca ccgaagagct agcttcttct gatttttcag
    15721 aaggtcaatt ggacaaaatg aaagaggaac tagagcaaaa agagcttcta cttcagcaac
    15781 aagccgcaga tcttcatgaa gcgcgtaaaa aaatcgcaaa agaattggaa aaatccattc
    15841 tgcatgaatt gaaaagtctg tatatggaaa acactgagtt tgaggtacgt ttcttaaaag
    15901 aagaaaatag acaattgaat catgatggat ttgaccaaat cgaattttac attacgacca
    15961 atccaggaga gccactgaag cctttggtga aagtcgcttc tggtggcgaa ctgtctcgga
    16021 tgttgcttgc tttgaaaact attttttctt ctgagcaagg tgtgacaagt attatttttg
    16081 atgaagtgga tacaggtgtg agcggaagag ttgcacaggc catcgcagat aaaatttctc
    16141 aaatatcgaa atactctcaa gttttgtgca tcactcatct gcctcaagtt gctgcagtcg
    16201 ccgactatca atattatatt gtgaaagcag tgattgatgg acgtacgcaa acttctgttt
    16261 cagagttgaa aacaaaagaa agagaagaag aaattgcacg tatgctggct ggaagtgaga
    16321 ttacaaaact cacactcgaa catgcaaaag aactattgaa actcgcaaaa aggacctgag
    16381 ccacctcgca gtggttcagg tccttttcag cagctgatag ctatatttta tttttacatg
    16441 acactcatta cagcagtcaa tacaacagat ccaatcgtta aaatcagcat tgtccaaatg
    16501 acaaattttg ttactttagc aaaagtactt gtttgttttt tttcacgcat tgttttcaac
    16561 ctcgtcatta attagttgat tcaagtgtac aatctttttt ccatttccac aagagggctt
    16621 ttttataaaa acttcccttt ttttaaataa taggtagtta cgatacaaat aaataatgtt
    16681 aatagaacta gccatataaa ggcatcatcc ctttcagcaa taggtaactt aatgttcatt
    16741 ccatagatac tgccgacgat tgtcggtata gttaaaataa tcgttaaaga agttaaaatt
    16801 ttcataatat tattcagatt attcgatacg attgctgaaa aagtctgatt catttgttct
    16861 actagtttta attgaatgcg agaagtagtc attgcctgct ctgtttccac caatacatca
    16921 cgtaaatgat ttttcaaagc atgattttct tggaaaagtt tattttgcgc tagttctttc
    16981 aatgcatcta aattagcaga agtcgcttct ctgaaataaa cgaggctttt ttggatatcc
    17041 atgatttgat aaagttgttt gttttcagtg gatacttgaa gtttggcttc caaagtatcg
    17101 cattgagatt gaagcgtagc taaataactg ttgtaagaga gtgtgacctg ccaaatgaat
    17161 tgaaagaaga tattgagatt aggcgaaata tcactttttg gaaaggtctg gctaaagact
    17221 ttctttaaaa acagtggttc atgattcgta attgtgataa gcttcttttt gggcgtcaaa
    17281 ataatagaga gcgggtatgt atccacttga aaataaccgc taggacttgt tttagcaaaa
    17341 ggatattgta acaataataa cgctggcttt gtcaactttg attgattgaa ttcttctgta
    17401 cgggaatttt ctgcatcgtc caatatggaa gtgatgtaat ctgacggaat ttcaaattga
    17461 tgtgtcagct gatgaatctc ttcttgatta ggacgttcta cacaatacca gatcgtttgg
    17521 tcagtttcct ttccggtatt acgcagtttc tcattatcta atgtaaaata tttgatcatg
    17581 attttcctcc tgttgcctgt ttcttttatt ataacggata gggaattgga atacatggaa
    17641 tgtgtttgta agatatagaa tataactgca taaatttaaa taaaatgaaa cagttgtgta
    17701 aagtatatac agtatacttg aagcaacgat aaaaaaggag tttacgttca tgggcacatt
    17761 cacaccactt atcttgtttt ggtatattta cccggtaatc gttttgtttg gttgccaatt
    17821 tcttgtaaaa ttattttcgt taaatcgtcg ttttaaaata aaagcccctg atttggcgat
    17881 tccttttttg tggggcggta tgcatgcatt gtctagaaat acagggacaa tttctatctt
    17941 accgtttttc tttatttctg ttttgctaat gggaatttgt attgctgttt tccaagcata
    18001 ttattatgaa gaaattattt atccgcgtta tttcaagatg acttggcgtt tggtttttct
    18061 gttgacaatc gtcttgtacg ctgtcttgat cattgtgaac atactttctt acctatgaaa
    18121 aaaatagaaa agctgtaaaa agtcttttaa aaggcttttt gcagcttgat ttttaaaaaa
    18181 atgtttatga ataaaaaaag ttgttcccgg atttcccaga aaaggtggta gaaagtgggg
    18241 gattgtggta gactaatttt agttagtgga ggaatggggg gctttcaatg ttgatgggtg
    18301 aattccaaca taatattgat gcaaaaggac gtttaatcgt gccctcaaaa cttcgtgaag
    18361 aacttggcga aaagtttgtt ctgacacgcg gattggatgg ctgtcttttc ggctatccga
    18421 tgagcgaatg ggaaaatctg gaagctaaac tgaacgaaat gccgttggct aaaaaagacg
    18481 cacgtacttt tgtgcgattc ttctattcgg ctgccactga atgcgaactt gataaacaag
    18541 gacgaatcaa tatacccagc acgttaagaa attatgcagc tttgacaaaa ggttgcgtag
    18601 tcatcggtgt atctaaccga attgaaattt gggatgagac acgatggcaa gatttttctg
    18661 cagcagcaga agagaacttt gacgaaattg ctgaaagtat gattgatttc ggactataag
    18721 aataggaaga ggctgactaa tgactgaaac atttcaacac tatacagtca tgttgaaaga
    18781 aacggtagat ggattgaaca tcaaaccgga tggcgtttat gtagactgta cactcggcgg
    18841 tgccggacat agtgaatatt tattaagcca actaagttct aaagggcatc tgtatgcgtt
    18901 tgaccaagat caaaaagcaa tcgatcacgc caagctgcgt ttagcttctt atgtcgaaca
    18961 aggtatggtc acatttatca aagcgaactt cagagaatta gaagaacgct tgagtgagca
    19021 tgtagataag gtagacggca ttttgtatga tctaggggtt tcctctcctc agctagatga
    19081 agcagaacgt ggatttagtt atcatcaaga tgcaccgttg gatatgcgca tggatcagtc
    19141 agctccttta tccgcttatc acgtaattaa tgaatattcc taccatgaat tagtaaagat
    19201 tttcttccgc tatggcgagg agaaattttc caaacaaatt gctcgtgaaa tcgaacgagt
    19261 acgaaaaaca aaaccaatcc aaacaactgg tgagcttgtc gaaatcatta aacaagtcat
    19321 cccagctccc gcaagaagaa aaggcgggca tcctgcaaaa agaatttttc aagctgtgcg
    19381 gattgcagta aatgacgaac tcggtgccga agaggcctct ttagaacagg caatccggtt
    19441 attgaaagtc aatggaagaa tcagcgtaat cacattccat tcattggaag acagaattgt
    19501 caaaagtatg tttaaagaat atagtacggt acaagatcta ccaccgggac ttcctgtcat
    19561 acctgacgaa ttccaaccag aactgaaagt cattactcgt aaaccaatcc ttccttcaga
    19621 ggaagagcta acagaaaaca atcgatcacg cagtgcaaag ttacgtattg cagaaaaaat
    19681 aagaatgaaa gaggagtaga atgatggcag aattaaagaa aatgcaagat tacgaatatg
    19741 atttacctga attggtggat cagccggaac aacaaatgcc tgattcttct tcgaatcaag
    19801 aaatcattct tccacaatca ccggcaaaac gattaaagca gatttctgct ttggaaaaaa
    19861 ttgctgtggc aagcgtggcg attgctattg ttgctttatg tgttttaaca gttatgctgc
    19921 gcacaaatat cagcggagct gaaaaagaaa tcacgtcaat acaaacagaa atcaatgata
    19981 aaaagcaaga aaaaacgagt ttagaacaag aaaaaaacga actttctcgt actgaacgga
    20041 ttaaacaaat cgcagaagaa aaaggattgt ctattaatga cgacaattta aggaaagtga
    20101 agtaaatgag tcttaaaaat aaaattcggc ggtttatgga aaagaaaaat ctaaatccga
    20161 tgaacaatcg caaaaaagta ggtatcattt tatttgctac gagtattgga ttgttctttt
    20221 tatttgccgt ccgtttctcg tatatcgtta ttggaggtca tgttgctggt acctcattag
    20281 ctgaaaaaac aaaacagttg taccaaggaa gtgaagtagt aaaagcaaaa agaggaacga
    20341 tttatgatcg taacggtgtg gcgctcgcag aagatgcctc ctcttattca attaaagcga
    20401 tactctcaaa aacatatacc tctggggata aaaaactgta tgttgaagaa aagaattttg
    20461 ataaaatagc agaaatcctg cataagaatc tttctataga caaaaaagat gcactgaata
    20521 ttttagagga cggcgcaaaa aaagagctgt atcaagttga atttggtagc tatgggaaaa
    20581 atatctccca agaaaccaag caaaacatcg aagcggacat gaaaaaagaa ggtgtcgctg
    20641 gtctttattt tgtcgaccat caggctcgga tgtatccaaa tggcgtcttt tcttctcatt
    20701 ttattggata cgcagttcca gacaaagatg agaatggctt agttggtaaa ctaggtttgg
    20761 agtctgctta caatgatatc ttgagcggta aagatggtaa gattatttat caaaaagata
    20821 attttcaaaa tcctctgcca ggtacagtgg cagaagaaga aaaagctgtc gacggacaag
    20881 atatctacac gactttagac agcagactac aaagttattt agaaacactg atggatcaag
    20941 taaatgaaga atatcagcca gaagaattga cagcagtcct gatgaaagca aaaaccggag
    21001 aaatcttggc aatgggacaa cgacctactt tcaatcctga aacaatggaa ggattgactg
    21061 gaaaagatgc tatctggcgt aatttcttgg tacaagatag ttatgaacca ggatcaacca
    21121 tgaaagtttt cactactgca gctgctatcg aagaaggaga attcaacgag aatgaaacct
    21181 tccaatctgg aaaaatccaa gtagaagatg ctacgatcaa tgaccatgac tttggtgaaa
    21241 aaggtgtgct gaccatgcgt caagctcttt catggtcaag taacgtcggg atggtcatac
    21301 tggaacagcg attaggcgga cgatggtata actatttaca aaaactggga tttggacaaa
    21361 gtactcattc cggattagat gatgaagtaa acggtgcttt acctacatta aatattgtcg
    21421 accgagcaat gagtgcttat ggacaagcgg taggtgtgac gaatttccaa atgatgaaag
    21481 ctttcacttc tattgcgaat aacggtacaa tgattcaacc tcgatatatc agcaaagtgg
    21541 tggatcccca aactggagaa gagcgaacaa ctcaaacaga agttctaggg caaccttttt
    21601 caaaagaaac aacagaaaaa gtccgtgaat atatgagaga tgttgtcgaa agcgaaaact
    21661 acggtagtgc ttatggtgtt gtacctggat acaatgtatc ggcaaaaacc ggaacagccc
    21721 aaatcgcttc tgatacaggg ggatatcaaa caggagatac agcctacctg tattcgattg
    21781 tggagatggt tccttctgaa gatccagact atgtcttgta tctaacaatg aaacacccta
    21841 agacatatga tcgaatggca ttggctaaaa tcgcaaatcc attgatgaaa cgggcaatgg
    21901 attttaaaga aactgaagaa gattcagata cagaaacaaa aacagaaaaa gtatccgtag
    21961 ccgattatcg taatcttgaa gcggacgttg cagcagctga tgctcaaaaa agtggactgc
    22021 aacctgtagt gatcggtaat ggtaaaaaag tacaaaaaca atctactgcc aacggtgatc
    22081 agttgatctc tggagaaaaa ctgatcctat atactggtgg tgataagctg atgcctgacg
    22141 taacaggatg gtcaaaagca gatatcatga agctggggaa aatcctcggt attgaagtta
    22201 gttttgatgg agatggttat tgtgtcaaac aagaacttgc tccatatgaa aaaatcactg
    22261 aagacaaact tagttttact ttagaagaat aagaagagaa ataggagaga aaagagcatg
    22321 gaatggacac aagcattgat tcccattgtc agcagttgtg caatgacaat tgccgcaatg
    22381 ccgctattta ttggttattt tcaaatgaaa aagcaaggtc aggcgattcg tgaggaggga
    22441 ccaaagtggc acaatagcaa agccggaact cctacaatgg gcggcttagt gtttctgatc
    22501 ggctctattt tgacaggaat ttgggttgga gcttggcaaa aacagctaac tcctactctt
    22561 ttcatcttgc tttttgtatt agcgttatac ggagtaatcg gctttcttga tgattttata
    22621 aaaatattca agaaaagaaa catgggactg aactcgaagc agaaattgat tggacagatt
    22681 ctagggggaa tcatctttta tcttgtttac cgttctgaag gttatccagg tgtattgaat
    22741 ttcttcggac tagatctacc tttaggaatc atttatggta ttttcgctct cttctggctt
    22801 gttggatttt caaatgcagt aaatctgaca gatggcatcg atggattagt tgctggctta
    22861 ggaagtatct cttttgcagc ttacggtcta attgcttggc atcagcatca gtatgatgta
    22921 ttaatcattt gcttaagtgt tttaggaggt ttactgggtt tcttcgtcta caacagaaaa
    22981 cctgccaaga tttttatggg agatgtcggt tcattagcgt tgggcggact tttagcggca
    23041 atttctataa tgctaaacca agaatggaca ttgcttttga ttggtttgat ctatgttatg
    23101 gaaacagcaa gtgtgatgct tcaagttact tcctttaagc ttacaggaaa acgaattttt
    23161 aaaatgtctc caattcatca tcactttgaa atgtgcggat ggtcggagtg gaagattgat
    23221 acagtttttt ggttgatcag cattgtgact tcgttgatca cattatggat cgtttggtag
    23281 gagatgacgg gaaatgaaaa aaatatcaac atatgaaaat aagaaagtgc ttgtcttagg
    23341 actcgcaaaa agcggagtaa gtgcggccaa attattacat gaattgggcg cacttgtcac
    23401 cgttaatgat ggaaaacctt ttgacgaaaa tccggaagca caagagctgc tttctttagg
    23461 gataaaagta ataacaggta gtcatccaat cgaattgttg gatgaagaat tttcattgat
    23521 ggtcaaaaat ccagggattc cctacagtca tcctttagta gcaaaagccc aagaaatggg
    23581 tattccggta attacggaag ttgagctagc ttacgaagta tcggaatgcc caattattgg
    23641 gattacagga acgaacggaa aaacaacgac aacaacgatg acaggactac tactgaatgc
    23701 aggagcagat caaggaattg cacgtttagc gggtaatatt gggtatccag caagtggtgt
    23761 agcgcaagaa gcaaaatcag aagataaaat tgtgatggaa ttgtctagct tccaactcat
    23821 gggaatcaca gatttccgcc ctcatatagc ggtgattacc aatatctatg aagcacatat
    23881 tgattatcat ggtacaagaa aagaatatgt caaagctaaa tggaaccttc aaaaaaacat
    23941 gacagaaaaa gattatctga tccttaactg gaatcagtca gaactacaag aattagcgca
    24001 aagaacaaaa gcacgcgtac tgcctttctc gacaaaagaa gtgttggaag acggtgtgta
    24061 tgctgacgat tactcaatat actataaaaa agaaaaaata atggaaatat ctgaacttgg
    24121 cgtaccaggt aagcataatg tagaaaatgc actagctgct atttctgtag caaaattgta
    24181 tggtatatca aatgaagcta tacgagaaac gttgcatttt ttccatggcg taccacatcg
    24241 tacgcaatat gtaggagaaa tccaaggcag aaaattttat aatgattcaa aagcaaccaa
    24301 tattttagcg actaaaatgg ctctaagtgg atttgaaacg tctaaggtcg tgttattggc
    24361 tggcggatta gacagaggaa atacatttga tgaattaatt ccttcattaa aaggaatcaa
    24421 agcaatggtc gtatttggcc aaacaaaaga aaagttgatg gatgctggaa aaaaagcagg
    24481 catcgagaca atagtaacag ccgattctgt agaacaagca gttcctttag cattagaaaa
    24541 cagtacagat ggtgatgtag ttttattatc accagcgaat gccagctggg atcaataccc
    24601 aaattttgaa actagaggaa acagattcat ggaagctgtg aaccgattaa aatagaaaaa
    24661 gtagaagcga gcgataaaaa atgaaaattt tggttacagg cggaggaaca gggggacata
    24721 tttatccagc tcttgccttt gtcaattatg taaagactca agaaccgaat gctgaattca
    24781 tgtatgtggg agcaaaacga ggtctagaaa acaaaatcgt ccctgataca ggaatgcctt
    24841 tccatacctt ggagattcaa ggatttaaac gaaaaatttc catgcataac atcaagacgg
    24901 tccgattgtt tcttaagagt atccgtcaag caaaaaaaat cctcagtgat tttcaaccag
    24961 atatcgttat cggtactgga ggatatgttt ccggagcggt agtgtacgct gcagccaaaa
    25021 tgggtattcc aacagttatc catgaacaaa acagtgtacc aggtgttacc aataaatttt
    25081 taagccgata tgtggatcgg atcgctcttt catttaaaga tgcggcttcc ttttttcctg
    25141 aaaacaaagc agttcttgta ggtaatcctc gagcacaaga agtagcagat actaaaaaat
    25201 cagatatttt gaaaacattt gatttggatc cagaaaagaa aacagtcttg atttttggcg
    25261 gtagtcaagg agcacttaaa atcaatcaag cagtatcagt ttttttaagt gaatatattc
    25321 cttcttctta ccaagttttg tatgcttcag gagatagata ttatgaagag atcctaaata
    25381 aaataaatcc tgaaatacaa aatattagtg tccagcctta tatcaaaaat atggcagagg
    25441 tcatggcttc cagtgatctg ctggtggggc gtgctggtgc aacttcgatc gcagaattca
    25501 cagctctggg gttaccggca gtgttggttc ccagcccata tgttacgaat gatcatcaga
    25561 ctaaaaatgc aatgagtctt gttcacgcag gagcagcaaa aatgattgca gataatgaac
    25621 taacaggtga atcactaagt cagacagtca acgaaatcat gggtgatgaa gaattgcaaa
    25681 agcaaatgtg ccgagcttct aaagaacaag gaatccctga tgcttcaaaa cgtctttatg
    25741 acttagtaaa acaaataatc aaaaaataaa ggcggtggtc ttagtgagta agaaaaaaga
    25801 accaaaaaaa ttgtctcatc cgtcttctga tgaaaacaat ttgactcctt ggcaaaaggc
    25861 taaccgaaaa tatctggcag aacaagagat gaaaaaccaa gagccaaaaa aagaagacat
    25921 aacattagat acaccccttt cagattctga agaagaaaaa acggttcaag aggaacagca
    25981 agaagaaagc cagtccgaga aaaaaatcga attttttgaa gagatcaagc aaaagaaaaa
    26041 cggtggacca tataacggtt cttttttgaa ccggctgccc aatttaaaaa gtcaacggca
    26101 caaagtacta tttcgtcggt tagctttgat catcactgta ttgggtatac cgctgatttt
    26161 tttgatctat tatgtttctc cttatagcaa attacaagca gttgcagtat caggcaataa
    26221 gacagtcaac agccaagaaa tcatctctga tacaaaattg tcattaggag aaaatgtttg
    26281 gggacagtat tttcatcgat ctacttatat cgatcgcctt aaaaaagcac agccaagaat
    26341 cgaaacagca aatatacatt tcaaagggat gaatgaattc gatttggatg taacagaata
    26401 taaagagatt gctttgatcg caaaaaacaa tcaatactac ccagtgattg aaaatggaac
    26461 agtattagat gaaaaagtag caaatcctac aaaaaatctc cctatcttgg aagagttcaa
    26521 agactctgca aaaatcaaag aattaacaaa gcaatacaat cagttatcat cagaattaca
    26581 aaaagcaatt tctgaaatca agtacacgcc aagagcaagc aataaaaatc tgatccaatt
    26641 gaatatgaat gacggcaatc aagttattgt aaatatcaat aatcttgcga accaaatgaa
    26701 atattattcg caagtagcca aagatatgga tgaaaaaggt gtgatcgaca tggaagttgg
    26761 tatcttctcg catccttacg gtagcactca agaaacggaa gaatcgggcc aatcggaaga
    26821 aacaactgaa agtgaagaaa atgcggatca aacaaacgaa tcaagtgaag aggcagaaac
    26881 gcaaaaagca caaacacctg gatcgtctag cacaaacgga tcatctgatt caaatgattc
    26941 aagtgaaaca agtcaaatag aagataatcc aagtacaact ccgtcaagtt taaatgaaaa
    27001 ttgataaaac aactttaaaa agtaagaatg tactttctaa aaaccagtaa tttatggtag
    27061 aattggaagc agtgaatata tctattaata agtatatttt aaaacggacg ctagttgttc
    27121 gcttgtatat ggaatagtaa gtaggaggga atcccattca tggcaaaaac aggaatgtat
    27181 gtaggccttg atattggaac gacatctgtt aaagttgtcg tggctgaata tatcgacagc
    27241 caaatgaata ttattggagt aggaaacgca aaatcagaag ggattaaccg aggcattatc
    27301 gttgatatcg ataaaacggt ccaagcgata caacgagcag tacgacaagc agaagaaaaa
    27361 gcaggtatcc agatcaaagg cgtaagtgtc ggtttgccag caaacatgct tgaagtagaa
    27421 aattgccagg gaatgattgc agttaacgga gattcaaagg aaatcaccga tgaagatgtc
    27481 agaaatgtgg cttcagctgc ccttgttcgt tcaatacctc ctgaacgtca aatcgtgtca
    27541 atattgccac aagattttac tgtcgatggg ttcgagggaa tcaaagatcc tcgtggaatg
    27601 atcggcgtaa gattagaaat gtacggtctg ttgtttacag gacctaaaac gatcatccat
    27661 aacatacgta aatgcgtgga aaatgccgga ttaatcgtca acgaaatggt gattactcct
    27721 ttagcgctga ctgaatcaat tctttctgac ggagaaaaag attttggtac gatcgtcatt
    27781 gatatgggtg gcggccaaac aacaacggct gttatgcatg ataagcaatt gaagtttacc
    27841 aatcttgacc aagaaggtgg cgaatttgtc actaaagata tctctattgt attaaatacg
    27901 tcatttaaca atgcagaagc gttgaaaata aactacgggg atgcataccc agaacggaca
    27961 tcaccagatg aagaatttcc agtagatgtg attggtcagt cagaaccagt gaaagtcgat
    28021 gaacgctact tgtcagaaat tatttcagct cgaatggaac agattttcaa caaagcaaaa
    28081 gatgcattgg accaaatcga agcattagag ttgccaggtg gcgtcgtatt gacaggcgga
    28141 gctgctagtc ttcctggtgt tgtggacttg gcacaagaaa tcttcggcgt aaatgtaaaa
    28201 ttgtatgttc caaatcacat ggggctgaga aacccggtat ttacaaatgt aataagtatc
    28261 gtggattatt cagcaaactt gagtgaagtg tatcaattgg caaaaattgc tgttacgggt
    28321 gaaacctcag cagctcgtca aatggtagtt gaacaagaaa caacaaacac atatgagagc
    28381 tatgaagcac ctgaagaaac aatctacgat gagccggaac caaaagagtc cggagaaaat
    28441 gtaaaaaata aaatcaaggg tttctttaca aatatttttg attaattcat cctgggagga
    28501 ataagatatg gaattctcaa ttgataataa tatcaatgac ggcgcagtaa tcaaagtcat
    28561 cggagtcggt ggtggcggtg gaaatgctgt aaaccgaatg attgaagaaa acgttaaagg
    28621 tgttgaattc attacagcca acacagacgt gcaagcatta aaaaattcaa aagctgaaac
    28681 agttatccaa ttaggcccca aatatactcg tggtttaggt gctggttctc agccagaagt
    28741 tggacaaaaa gcggcagaag aaagtgaaca atccttacgc gaagctttag acggtgcaga
    28801 tatgattttc atcactgcag gaatgggtgg aggaaccggt acaggtgctg caccaatcgt
    28861 tgcaggtatc gcgaaagaat tgggcgcatt gactgttggt gtcgtgacac gtccattcac
    28921 ttttgaagga ccgaaacgtg gccgttttgc tgcagaaggt atcgctcgtt tgaaagaaaa
    28981 tgtggataca ttattaatta tctcaaataa ccgtttatta gaagttgtag ataaaaagac
    29041 accgatgctt gaagctttcc gtgaagcaga taatgtttta cgacaaggtg tacaaggtat
    29101 ctctgatttg atcacagcgc caggttacgt taaccttgac tttgctgacg tgaaaacagt
    29161 gatggaaaac caaggtacag cattgatggg gatcggtgtg gctagtggag aagatcgcgt
    29221 agtagaagca acgaaaaaag caatctcctc tccattgctt gaaacatcta ttgatggagc
    29281 tgaacaagta cttcttaaca tcacaggcgg tttggatatg acattgttcg aagcacaaga
    29341 tgcttcagat atcgtagcaa atgcagcaac tggcgatgta aatatcattc taggtacatc
    29401 tatcaatgaa gaaatgggcg atgaaatccg tgtgacggtc atcgcaacag gtatcgatga
    29461 atcaaaaaag gaacgcaagt ctagccgacc agctagacaa gcacaaatgc aatcacctgc
    29521 tcaaaagact gtattagata tggatcaagc taaaccaact tcttcagaag aagaaaacag
    29581 ttttggcgat tgggatatca gacgtgaaca aaatgttcgt cctagagttg atgattccaa
    29641 ttttgacaat atcgaaaaaa aagaatttga tacattcaac cgcgaagaaa caaaatctca
    29701 tggcgatgat gaattaaata cgccaccgtt tttccgtcgt aaaaaataaa ggagaggaag
    29761 agcacatgat tgctgacaac ttgcagaaaa tcaagcaaga aattcaggat tcgtgtgctc
    29821 ttgttcatcg tgatccgaaa gaagttacat tagtagctgt aacaaaatct gttgaatccg
    29881 ctcaagctga agaattggtt agagctggcg ttatgaattg tgcagaaaac cgagtagata
    29941 aattattaga aaagaaacaa gatttatttg attttcctga aataaagtgg catttaattg
    30001 gtaatttaca gcgcagaaag gtaaaattga ttgtaaatga aattgattac tttcatgcgt
    30061 tggacagttt aagattagca gaagaaatcc agaagcggtt agagaaagaa ttatcctgtt
    30121 ttattgaagt aaatgtttct ggtgaggaat caaaacatgg aatttcacca gatgagcttt
    30181 taccgtttgt ggaatcttta gctgcctgca caaaaatcaa ggttgttggg ttgatgacta
    30241 tggcgcctaa agaagcttct gatgagcaaa tccataaagt gtttggtacg ctgaaagaat
    30301 tgcgtgatct tgttcaagca aaaggttatg cccatgcacc atgtagtgaa ttaagtatgg
    30361 ggatgagtca agactttcaa attgcgattg aagaaggtgc aacatttatc cgtgtaggaa
    30421 cagcgctatt caaagacgaa taaaggagga acaaaaatgc cgcttttaaa cagaggagca
    30481 attgctaatt ttttcggatt agctgatgag gaagagtacg aataccaaga ttatcctgaa
    30541 acaacaaaaa aggctgtaca gccaactaaa aaaaatacaa cacagaatca atcaactcat
    30601 tctgtatctg ctgcgaatac tcatgaaaaa caaaaaacgg cgacttcacg tcctgtaacg
    30661 catgaaagta ttcagccggc tgaaaagaaa aaagtaattg aactacatca atcacctaca
    30721 aaaactgtag cgaaagaaag aacagcttca agaacctcta cacaacctaa agcaaatctt
    30781 gctggaaaaa tcactgtaat ggaaccaaga gcttattctg aagctatggc gattgctaaa
    30841 cgaatcattg ctggagaagc aaccttagtg aattttcatt tagtagaaga aaaacaagcg
    30901 agaagaatcg ttgattttct aacaggaaca gtttatgcac tagatggaga tatcaaacgt
    30961 gttggagatg agatttttct ttgtacaccg actgggatgg agattgacag ttcaaccgct
    31021 caatcttttg cagatgcgaa tttgtttgat ctgtaagagg aggaactacg atttatacta
    31081 ttgcactatt acttcattta cttcaacggg cagcctattt ctatagcatt atcttgatgg
    31141 tatatgcatt gctctcttgg tttccaggcg ggtatcaatc agggctagga cgattattag
    31201 ctaagatctg cgaaccgtat atcagtttgt tcgatcgtct gcctttgagg attggaccct
    31261 tggatttcac aattttaggg gcaatcctga tcttgaactt agccgtacaa ggtttagcac
    31321 agatcgttat atggataact gtccatttag tttattgatt gaagcaacaa aatctttatt
    31381 ctcagaatag ataggagggg aaaattttgg atgcaaatgt ataccagcac tttcgggcag
    31441 atgaacgtac atttatcgat tcggtaggcg attggattga acaggttcaa ggacaatatg
    31501 caccctatct gaccgaattt cttgacccaa gacaatccta tattttagaa acactgattc
    31561 gacaaagcag tgatttgcgt ttcatgtttt atggcggata tgaacaggca gaaagaaaaa
    31621 gatgtttgat tttccccgat tattacgaac ctgtagagga agattttgaa acagcattgt
    31681 atgaaatcca ttatcctagc aaatttgcca gcctaagtca tgggaaaatc cttggaactt
    31741 tagtgggaac ggggatcaaa cgctcttact ttggcgatat catctcggac ggtgatcgct
    31801 ggcaagtatt tattgcaaat gaggtggatc attttgttgc tgcacaagta acaaagattg
    31861 gaaaggttgc tgtacgcttg gaaccaatca aatacacaga aatgattatg ccgaaagata
    31921 gctggacaca agaaagagga acagttagtt ccatgcgact agacagtgtg atctctgaga
    31981 tttataatat ttccagacag cggtccaagc agctgatcga agctggtaaa gtaaaagtga
    32041 actggacaga aaatacaaga cctgattttg tacttgaact attagatatt atttcagtca
    32101 gaggttatgg cagagtccag atccaagaac ttgaggggaa gacgaaaaaa gaaaagtaca
    32161 gggtactgtt cggtgtatta cgtaaataaa ataaaaaggt ggtttactca tggctttaac
    32221 tccattagat atccaaaata agacattccc gacaaaaatg cggggatata atcaagacga
    32281 agtagatgat ttcttagatt tagttgttcg tgactatgag gaattgactc aaagaaatcg
    32341 agaattagaa aaagcagtca aacattctga agaaaaactt gagtacttca atgaattaaa
    32401 agatgctttg aaccaatcaa tcatcgtagc tcaagatacg gctgataaag taaaaacaag
    32461 tgcaagcaag gaatccgaag tgatcgttac ttctgcacaa aacaaagcag atgagttagt
    32521 tgcgaacgct gaaaaacgtg cacatcaatt aacaactgat gcagaagaaa aagcaagaaa
    32581 aatcttgaca gatgcaacag aaaaagcaag acagcttgcg acagaaactg aagatcttaa
    32641 gaaaaaaaca cgagtattcc atcaacgtat cagcttgatg cttgagtctc aattagaaca
    32701 agtgaaaagt ccggaatggg atgaaatttt acaaccattc tccagctacg tgactgacag
    32761 ccatgaagtg atcaaagaag ttttagcaaa acagcttgac aatgaaaatg atactgatgt
    32821 aaactcagac acaggagtca tagaagcacc tatgacggca tttgatacac aggctattga
    32881 tatctcgatc gtgcctggtg aaaatgaaaa cgagtagaac agaaaagcac tttgttattc
    32941 ttaaagcgag ccgatgagag tggaagttcg gtaagacact gcatggtgaa agatcctcta
    33001 cttgagacct gcttttccga aattagagta agaagcagcg gctgacctcg ttattggtca
    33061 cagagagttt gcactagata gatgcaacta aatttgggtg gtaccacggc atgttcgtcc
    33121 cttgcggctg aacatgcttt tttgtgtccg gaaaacaaat aaaggagtgt atggttgaat
    33181 gaaaatgaaa gaaaccttgc aattaggaaa aacaaaattc cctatgcgtg gtaatttgcc
    33241 aaaccgagaa ggcgagtggc aaaaagaatg ggaagaaaat cgaatctacc aaaaaagaca
    33301 agagttgaac gaaggaaaac ctagctttgt tcttcatgat ggacctccat atgccaatgg
    33361 taatatccat ttaggacatt cattgaataa aatcagtaaa gacattatca ttcgttcaaa
    33421 atcaatgtct ggctatcgtg caccatatgt tccaggatgg gatacacatg gattgccgat
    33481 tgaacaagta ttggctaata aaggcgtgaa acgtaaagaa atgtccatgg cagaatactt
    33541 ggaaaaatgt cgtgaatatg cactttccca agtagataag caacgagcag atttcaaacg
    33601 cctaggagtt gcaggagatt gggaacatcc ttatgttaca ttagatcctt cttatgaagc
    33661 tgcacaagta cgcgtatttg gtaaaatggc tgaaaaaagc tatatttaca aaggattgaa
    33721 accaatttac tggtctccgt caagtgagtc ttctttagcg gaagctgaaa ttgaatataa
    33781 agatgtaaaa tctccatcta tttttgtagc tttcaatgtc aaagacggca aagatattct
    33841 tgatgaagat acagcttttg tcatttggac gacaacacca tggacattgc cagcaaacca
    33901 aggtattgct gtaaatccaa aatttacgta tgtattggtg gaagccgatg gccgtaagtt
    33961 tgtcgtagcg aaagatttgc tagaaacagt ccaatcagaa atcggttggg aaaatgtaac
    34021 agttctaaaa gaatttgctg gacaagaaat ggaatatatg actgcaagtc atccattcta
    34081 tgaacgtgaa tcacttgtga ttttaggcga tcatgttact ttagacgctg gtactggatt
    34141 agtccatact gctccaggac atggggaaga tgactatatc gctggtagca aatataaact
    34201 atctgtagtg tcacctgtag actcaaaagg attgtttaca gaagaagcac ctggatttga
    34261 aggtatcttt tatgacaaag caaatcctat gatcactgat ctattaaaag aaaaaggagc
    34321 tttgctgaag ctagatttct ttactcatag ttatcctcac gattggcgga cgaaaaagcc
    34381 tgtgatctat cgggcaactc cacaatggtt tgcatctatc gataaattcc gtcagaatat
    34441 tttggatgaa gtagaaaaag tagagtgggt tatcccatgg ggtaaaacac gtttgtataa
    34501 tatgatccgt gaccgcggcg attgggtaat ttcccgtcag cgtgcatggg gagtaccact
    34561 tccgattttc tatgctgaaa atggagaagc aatcatcaca ccagaaacaa tcgaacatgt
    34621 ggcaaaatta tttgctgaac atggatcaat tatctggttt gaaaaagaag cgaaagaact
    34681 tcttcctgaa ggatttaccc acccaggttc tcctaatggc gaatttacaa aagaaaaaga
    34741 tatcatggat gtctggtttg attctggttc ttctcatgaa gctgttttac gtcagcgacc
    34801 agaattatca ttcccagcag atatgtattt agaaggatca gaccaatatc gtggctggtt
    34861 caactcaagt atcacgacta gtgtggctat caatggtgta gcaccataca aatcagttat
    34921 ttctcaagga tttgctttag atggtgaagg ccgtaagatg agtaaatctt taggaaacat
    34981 cattgcacca gaaaaagtga tcaaacaatt tggtgcagat atccttcgct tatgggtatc
    35041 tagtgtagat tatgaagcag atgttcgtgt ttctatggat atcttaagtc aagtggcaga
    35101 agtatatcgt aaaatcagaa atacgatgcg tttcttatta gcgaatacag aagatttcga
    35161 tcctaaaaaa gatactgttt cgtacaatga tttacgttct gtagacaaat atatgaccgt
    35221 tcgattgaac caagtaatca aagaaatccg agaagaaggg tacgacaaat ataacttcat
    35281 gcatatttac cgtacagtta tgaacttctt gacggttgat ctatcgtcat tctacctgga
    35341 ttttgctaaa gatgtagttt atattgaagc agaagatgat tatcaacgtc gttgcatgca
    35401 aactgttttc tatcaaacag ctgtagcttt gacaaaatta ttgactccta tcattccgca
    35461 tacagcagaa gaaatctggt cattcctgaa agaagaagag gagtacgttc agttaagcga
    35521 gtttcctggt tatgaagagt ttgcaaacca agaagaattg atggatacct ggactgcttt
    35581 catggacttc cgtgataatg tcttaaaagc attggaagaa gcaagaaaca gcaaattgat
    35641 tggtaaatct ttggaagcaa aactaactgt ttatccaaaa caacaagttc gtgaaatgtt
    35701 aaaagctttg gatgcagata ttgcacaatt attgatcgtt tcaccagact actttgaagt
    35761 tatggcagca gatgaacaag tgccagataa cgctatggta tttgatgatg ttgctattac
    35821 agtggaaaaa gcagacgggg aaacttgcga tcgctgtcgc caagttcgta aagatgtagg
    35881 agtagatgaa aaacttcctc atctatgcgg tcgttgtgca aaaatcgttg aagacttcta
    35941 tcctgaagct gtagctgaag gattcgaaga aaagtaaaag ggaaaagggg gtgtgacatg
    36001 ttctttgtca cacccccttt tgttatcaat cgtttaattt tccacgttca cgataccagt
    36061 tatctggttg ccagatccat tcgaatccgt ctttttcaag tagttcgaaa gctgctttag
    36121 gtcccattga tccagctttg tagttgggaa aatccggttg tgtttggtcc caagcttttc
    36181 gaatgacatc cacgatatgc caagattgag cgacttcttc ccaatgagag aagttcgttc
    36241 catcaccatt caacgcatct aatagtaatt tttcgtaagc ttctgggcta ttttcaacca
    36301 tttctgcact atttctaaat tccaatttga ttggttccgt ttcaaatcct tgccctactt
    36361 ctttcccatt caaggataaa gaaaatcctt cagttggctg aatatagatc gttagaacat
    36421 taggtggaag tgtcgtatcg tcacatggtt catcgacaga tgttttgaat acattaacag
    36481 gaacttgttt gaatacgatg ttgatccgag tccctttttc agtcaaacgt ttgcctgtac
    36541 gtacatagaa aggaacacct gaccaacgga agttatcgat agtgaatttg cctgcgacga
    36601 atgtttcagt ctctgattga gcatccactt tgtcttcttc gcgatagccc ttgaattgta
    36661 caccatccaa ttcaccttga gcatattgcc cgcgaacgaa gttttctaac acttcttctt
    36721 cgttataaat gcggatagct ttcaatgctt tgatcttttc tgtacggatt tctttttcgg
    36781 aaaatgctac tggcggttcc attgcaagta atgctacaac ctgtaaaata tgattttgca
    36841 ccatgtcttt aagtgcgcca ctatgatcgt agtagccccc gcgatcttct acaccgaggt
    36901 tttcgccaaa agtaatttgg atattgtcga tatagcgatt gttccattga gcctcaaaaa
    36961 tattattggc aaaacggata gctgagatgt tttggatcat ttcttttcct aaataatgat
    37021 caatcctgaa aatatcttgt tctgggaata cttctctgat ttgctggttc aattcaaaag
    37081 cagactggta atcagaaccg aacggttttt ctatgatcaa acgattgaag ccattttttg
    37141 tcataatatt ttgtgatttc aaatgagaaa cgatcgttcc aaaaaattga ggtgccatag
    37201 ccaaataata gacacggtta ccgtttaatt gatagtgtcc atctaatcgg tcagacaatt
    37261 cttttaatgt ctgataatgc gttgaatcat ttacatcatg ggattgatag taaaaatggc
    37321 ttgcaaaggt agttgcttct tcatctgaag gattcaatgc acggattgtt tctttgacaa
    37381 cttcacggta gtgctcatcg ctccagggac ggcgagcggt tccgattaca gcaaaatgct
    37441 cgcctaaatt tccttttctg taaaggcgga acaaagacgg gtacaattta cgctgggcta
    37501 ggtcacctgt accgccgaat atcgtaaaca atacattttt ttcattagac atgtggatac
    37561 actcctttat attttatcga caaaaatcgt gctggccgcc ttaaagctga cggggatttt
    37621 ctttctatca ttttggatag tgatcggtcc ttcataagct gcgatgtcaa cgatctcata
    37681 ttcttcgttg atattcaggt caatagtgac taagtaatcc aacaattctt tttcatcaag
    37741 cactcgtgcg atacgcactt ttgtaccgac aggataggac tccaatgttt gtcggcgttc
    37801 ttcatggaca acttcctctt ttttaggaat cgatcctcca tgaggacaat gttcaggatg
    37861 attcaaatag ttttctaaat gatctgcaag atgttccgaa gtcacatgtt ccaatacttc
    37921 agcatcatca tggacttcat tccagctata gtctaaatgt tcaactaaaa aaacttccca
    37981 taagcgatgt ttgcgaatca gactgctagc tttttgcagt ccgcttgcag tcaattgtac
    38041 accttgataa ggggaatgtt ctacaagctc ttctttcaca agtttagaaa tcatttcact
    38101 gacagatgca gctgagacat ctagactaga tacgatttgt ttattgttga ctttcgttgt
    38161 atctccacct agttctaaaa tgattttcaa ataatcttct cgatttggtg tcatattcga
    38221 tcatcctttc atggtaaaaa accatcatta gtaagtgtaa caaaaaaaac gttaattgac
    38281 tatttttcta aataaaaaac aaaggatttt tagacgtttt tacaacaaaa aacgaaagat
    38341 gatagagaag actcattctt tcgtttataa aggatatata tatacattgg gagggatcta
    38401 tttttcagat aacaacttat atgaagtgtc tgcagattcg tcataaagag tttgttcggg
    38461 ataagaaaag ataagataaa aaccatactg atcacgtacg actaaacgat attcatctat
    38521 ttctaccaca gatgtgccaa gagactgacc gttgatcaaa aacgtgtaat cagatgtaat
    38581 ctgtaattgt tgtttttgct ggctatcact aaagaaccat gtaccttgat actttgagag
    38641 tccttctgac ttttccgctt gttttttctt tttcaagtaa ataaaacttg agagaacggt
    38701 aatgagtgaa ccaagaaaat agatcatttt ttttatcacc aaatcactcc tttatgagaa
    38761 aataatcgca tagacatatt ctttcttctt tatttcattg taccgaataa aagctaaaat
    38821 ttctctaaga ctttcgactg gtttttttac tgtttttcta caaattatga taggatacat
    38881 agtaggaaga aaggtgacat aatttgaagt atcagcaagt ggaaatagct ttttttattg
    38941 accgcccgaa tcggtttata gcacattgta tgaaaaaaga cggagaaata attattgcgc
    39001 atgtcaaaaa tacaggtaga ggaaaagagg tcctgatacc aggcgctgaa gtggctattg
    39061 tatttgctcc tggacctaaa cggaaaaccg cctatgatct gattgctgta aaaaagaagg
    39121 gtgactggtt taatatcgat agtcaattgc ctaatcggct ggcaattgat ggtattttag
    39181 acggcacgat ccttttacca ggattaactg gtccatttac ggtatacaag cgtgaagtaa
    39241 cttatcttca ctccaaattt gatatttatt tagagacggt ggtcggacaa aaagcatttg
    39301 ttgaagtcaa aggtatgaca cttgaaaata aagcgattgg tgcttttcct gatgcgccca
    39361 cactcagagg attgaaacat ataggagaat taacgtatgc cgcacaagat ggttatgccg
    39421 cttatgtctt gtttattgct cagttcgaac atcttcatct ggcaacgatc catgaagaaa
    39481 tgcaaccagc actagctgat atggttcgac atgcgcagca aagcggagtt caaatcctcg
    39541 cctataattg tcaagtatcg gcagatgagg taactgtcaa gcaggccatt ccatttgatg
    39601 tagatatccc ttttgaagat cctatacaat agaaagaagg taaaataatg atcaacttag
    39661 gtattatcgg aacaaattgg attactcatc aatttgtcca agcagcttta agcacaaaaa
    39721 aatatgaatt gacagcggta tattctagac atttggaaaa agcacaggaa tttggttctc
    39781 attatgaagg ggatatcgaa ttcgcaacag atttgaagac ttttttcaat attactcata
    39841 tggacaccat ttatattgct tctccgaata gtcttcactt tagtcaagct aagcaagcaa
    39901 ttttggcagg aaaaaatgtg atagttgaaa agccagcttt ttcgacgcca aaagaaatgg
    39961 atgaaatcat ccatttggca aatgaacaca atgtgtattt ttttgaagca gcaagaaata
    40021 tccatgaatc aggttttaaa aagattgccg attacttacc attaaaagac gagataatcg
    40081 gtgcaaattt cagctatatg aaatattctt cgcgctatga ccaagtatta gatggagaag
    40141 aaccgaatat cttctcgact catttctcag gtggtgcatt gatggattta ggtgtttacc
    40201 ttgtatatgc agctgttgct tggtttggaa tgccaaaaga agtccattat ttcccaagaa
    40261 agatttctac tggtgtagat ggtcttggat gggggatctt acgttatgag aattttgatg
    40321 tatcgatcca gccaggtaaa atcggtgatt cttatttacc gtcagagatt tattttgact
    40381 caggaacgct agtgatgaat ggagtcaatg caattgaaca agcacaattt catgatcgcg
    40441 cgcataaaga aatcattgat ttggcggtaa cagctgcaga aaatccaatg gcagatgaag
    40501 cagaggattt tgccaatgtc atggctcatc cgaaagatcc agcttgggga ttgttgtatg
    40561 aagagtggat cgagctagct agaaatgtga accaagtgat ttatgattta agaaaaaatg
    40621 gcggcatcaa attcgatgca gataatgagg aagattttta atgaacgaat ttgtacaagt
    40681 atttcccgaa aattggcaga aaaaatatga ggataaaggg tttatctcac cttctcttat
    40741 tcaacgagca gtttttcagc cactaagtaa taaacaaagt atagtagcgg tttctcctac
    40801 aggttcaggt aaaacactgg cttatctttg gcctctacta ctaaacgtgg aaccaggtga
    40861 agcaagtgcg ttggtcattt ttgcttcttc tcaagaacta gctattcaag tagcagatgt
    40921 tgcacgtgaa tggggaaaag acaaagagtt gaaagtccaa tctttggtgg gcggagccaa
    40981 cgtcaaacgt cagattgagg gtttgaagga aaaaccagaa atcttgattg gtacaccagg
    41041 aagaatattg gaattgatga aagcaaaaaa aataaaagca catcaagtga aaacaatggt
    41101 atttgatgaa gcagaccagc tatttgacgc tgggaacagc caaatcattg atcaaattct
    41161 tcatcaagct ccaacagagt atcagctagc ttttttttca gcgacagcag atcgttccct
    41221 tgaaagcatt gaaaaaatca ctgggaaaac gtttgagaca attgacgtga cagcagagga
    41281 cgattctcga aaaggactga gacattactt tttacgtgta ccgatacgaa agaaagatga
    41341 ctacttgaga cgtctcacgc atatagaaga ttttcatggt ctgattttct ttaatcaact
    41401 gactgaatta ggcgtaatgg aagaaaaatt gctgtataga ggtgtaccgg taggaagtct
    41461 ggcctctgat cagaacaagc tactccgaaa agcagcgcta gaacaattca aaaagaaaat
    41521 ggtcagagca cttttgacca cagatgtggc ggctagaggt ttagatatta caggtcttcc
    41581 ttatgtagtg aatgcagaag ttccgttaag tgaagaagct tatttgcatc gttcgggaag
    41641 aactggtcga atgggtaatg aaggaatagt aattacactc gtacaagata ataatctaag
    41701 agatttaaaa cggcttttac gtccatcaaa tataacactt gaagagattt ttttatacgg
    41761 aggtaagctg caaactgagc agccaaccaa agaagatgca gctcctgcag taaataaaaa
    41821 gtatgcgaaa actttttctg gaaaggagca gaacccagaa tataacaaag aaaaaataag
    41881 aaaaaaagtt aaaaataaat caaaaaaaga gagaaataaa ggagcaaggc gcaaataagc
    41941 gcctttttat gctatgatta tgaaaacggg aacaaaattg gttgccagag gaggggttat
    42001 gtgaaagaac gtcaagaaga gatcttgcag ctattgacag aaaaccaaca aggattgaca
    42061 gcaagtgatg tagctgagcg cttaactatc gatcgaagca atgcaagccg atatttaagt
    42121 gaactataca aggcccatca tattgtaaaa accgctggcc gaccagtcgt ttactctttg
    42181 ccaacggaaa agtcaaaatc tgatgaagtg catgtcgact cttctacgca agtgactttt
    42241 gaaacactcg ttggagaaaa tgattcttta aaagtcagca tacagcaggc aaaagctgcg
    42301 attctttatc caccgcgggg gttgcatacg attatttttg gagaaactgg tacagggaag
    42361 tcgatgtttg ctgaatgtat gtatcatttt gcaatcgatt cagagatgct ttctgcagat
    42421 gcgccatttg tttctttcaa ttgtgcggat tatgctcaaa atccgcaact gctctttgga
    42481 cacatctttg ggatcaaaaa aggggcttac acaggagcag ctcaagatag tcccggtcta
    42541 atagccaaag cagacggcgg tatattgttt ttagatgaga tccaccgttt accgccggaa
    42601 gggcaggaaa tgctgtttac tttcattgat aaaggtattt accgtccgct tggagaaagt
    42661 gcacaagtac atgaagcatc tgttcagatc attggggcga cgactgaatc ttcagaaagt
    42721 ttcttatcta cgttcaatcg acggattcct atggcaatca ccttgccgaa tcttgcttca
    42781 cgttcactgg atgagcgtta tgaaatcatc tctcttttta tcaaacaaga agcgaatcgt
    42841 ttgaatcaac gtatcgatat cgaaaaagaa gcgattcttg ccttcatgct ttatgatgcg
    42901 gaaggaaata tcggtcaagt gaaacgagac ttgaaactag tctgtgcaaa atctttttta
    42961 cattaccgta ctcatcatga gaaaaagcta atcattcgaa aagaagaact ttcccttcaa
    43021 gtacaaaaag gattactgaa aatcaaggaa ataccggaac gtctcgaccg attcatggac
    43081 agcaaaagtc agtatttgac atttgaacca ggatttgctg atgttgtctg gtcacaagat
    43141 ccagaacgaa acatgcaggt ctataacgat attgaggaaa aagtcacttc tctttccgaa
    43201 accggtgtgg agaacattga tctagagacc ctcatttcaa aagatgtgga tgcttatttc
    43261 cagacatatg tcaaagaatt gactaaaagt tcgattccca aagatctttt gccagatgaa
    43321 atctggcggt taaccaatca attgtatgat gtggctgaaa aacggttaga ccgtatttat
    43381 aatgaaaaag ctcgttttgc ttttgctttg catctgcaaa gtacattgga tcgtgtgaga
    43441 gaaggtcata tgatcgtcca cccagatttg aataacgtac ggaaaaattt taagagggaa
    43501 ttccaggtag caatcgatct ttcaacgatc attgaagaag aagaacaagt tgagattcct
    43561 tttgatgaaa tcggctttat tagtatgttt ctttctatca atctaggaga aagtgagcct
    43621 gttaaagaaa acaaagtaga tgttctcata ttgatgcacg gcagacagac tgcttcaagc
    43681 atgttggaga tggcacaaga actattagga acaaaagtag ggaaagcttt taatatgcca
    43741 ctggttatgg aagttcaaac aatgtatgaa gaagtgttac aatatgtcaa aaactatcaa
    43801 gagcatttga cgaatggcct actcttgtta acagatatgg gatcattaaa caccttcagt
    43861 gatttgattt atgaagagac tggcattcgg acgaaagcta tcacgatgac aagtaccatg
    43921 attgtgatag aagcgatacg gatggcaagt gttggaagaa gcttagaaga tatttatcaa
    43981 aatatccagc tttcctttga atcgatcgta cgtgatcagt tcaaagcgga aaagtctgaa
    44041 actttacagc gtaaaaaagc cgtaatcgtg acatgcttta ctggtgaagg agtagctgcc
    44101 aaattatatc agcgtattat gccagtgatt aatcaaaata aggtagaact gatccagatg
    44161 cagtttttag aacgtgaatc attcaaaaaa cacatcgatg ccttattgga agaatatgag
    44221 atcaaagcaa ttgcgggaac agttgatgtt gattatcaaa atatcccatt tttttcagcc
    44281 tatgacgttt ttgatgatga aaaactaaat attttaaagc gtatagctga tgatgagatc
    44341 ccgacagcta aaattgtcga ttcacttgct ggatcattaa gcgatgtctt ttctattgat
    44401 gaactgatcc gttcattgca aaagacagtt cagcagatcc agaccgattt gtcgattatc
    44461 gtggaacctg gagtagatac tggaatcgtt atccatttag ccttcatgtt agacggattg
    44521 atcaaacaag aaagccatcg gcaatttgag aaactgtcta ttttctcaaa aatttatcgt
    44581 ttggaaatgg atatcgttcg ggcaaacttg atggcactag aacgaaaata tcaggtaaaa
    44641 attccagaag atgatatcgc atttcttaca caaatgtttc ttgaaaataa aataaaaaaa
    44701 tgaacacact aatcatactg tttacacact aaacatctgt gtgcgcacta taaaaaagag
    44761 ctaaaaagca tgaaaacgct atttcagctc ttttttttat tggcatgctt cttgcgttat
    44821 ataaaagtgt aaaaaaataa aaggagtgtt aaaaatggca aaaaaaacaa tcatgttagt
    44881 ttgttcagca gggatgagta cgagtttatt ggtcacaaaa atgcaaaaag cagcagaaga
    44941 aaaagggatg gaagccgata tttttgcggt ctctgcctca gatgcagata ataatttaga
    45001 atctaagaat gtcgatgtat tattattagg tcctcaagta cgtttcatga aagctcaatt
    45061 tgaacaaaaa cttgcgccta aaggtattcc attagatgtc atcaatatgc aagactatgg
    45121 catgatgaac ggagaaaaag ttcttgcaca agcagaaaat ctaatgaaat aaatcaagta
    45181 tgcagttggg ggtataacaa cgtggaagat caacaaaatc tagaagccat catggggtta
    45241 atcatgtttg gcggtaatgc aaagagcgat gcaatggaag cgattgcggc tgctaaaatc
    45301 ggtgattttg agttagcgga taggaaaatc gctgatgcag aagaatcact tatacaagcg
    45361 catcatgcac aaacagacat gttgacacaa gaagcacaag gaaatcatat gcaagtgaca
    45421 ttattgactg ttcacagtca agatcactta atgacatcta ttgcgtttac tgatttagca
    45481 aaagaaatca tcgaacttta tcgtcgcgta tacagcgaat aaatacaact tcttttttga
    45541 acggaagaag aaaaattttt gaacggattt tttattttgt tataaggaga actctcttta
    45601 tataaaaaac ttataggagg aagtaaaaaa tgaacggact atcaagttgg atggagaaat
    45661 acatcctgcc ggttgccgca aaaatcggag cgcaaaaaca tttagttgct ttgcgtgatg
    45721 cttttatcgg cacactgccc gcaacaatgg cgggatcagt cgctgtaatg atcaatgcaa
    45781 ttattcgtga tttaccgcaa cagtttaaca gtacctatgc tgacagttta gcagcagaca
    45841 aaggaatctt tgccgtattg aatgtcatca tcggaatcaa tggttttgtc tggaacggta
    45901 cactagcaat cgccggattg atctttgcct tttcttgggg gtataatctt gcccgagctt
    45961 atggagtaaa tgatctagct ggggggatcg ttggtttagc taccttgatt caaggtattt
    46021 cctttggtcc agcattatca gcagcattag atgtgaatgt tccttcaaat gtcgcagatg
    46081 caatcaatag ttctgagatt ggtgcaacga ttgcagatgg cacactgtca gttggattat
    46141 ggggttggct aaaactagat catttgaatg gaaacgctta ttttacagtt atgatcatgg
    46201 gagctatttc agtcattatt ttttgtaaat tgatgcttgc caacattacg atcaaaatgc
    46261 ctgattcagt accaccagca gtatctaaag catttgcagc cattttacca gcgacgatcg
    46321 ctttatacgt cattgctatt atcaattata tcgtaggtcg tgtagctgat gggcaactga
    46381 tcattgattt ggtgcaaaaa tatattgcag aaccattcct aggattatct caagggttgg
    46441 gagcagtatt gatcgtcaca gtatttgttc aagtattctg gttctttggt attcatggac
    46501 caaatgtatt ggcaccagtg ctagagggaa tctggggaca agcacaacta atcaatattg
    46561 atattttcca aaaaggttat aacggaaaaa ctggtacacc agccgtgtta gatgcaatcg
    46621 aagatggaaa agcttatatg tgggtccgcg gatcgttcga tgcttttgca tggtttggcg
    46681 gatcaggcgg aacgatcgtc ttactgatag ctatcttgat tttctctaaa cgtgcagatt
    46741 atctaacagt agctaaatta tccattgggc ctggtatctt caatatcaac gaaccagtga
    46801 tgtttggtct gccaatcgta ttgaatgcaa tcatgtttat cccattcttg attgctccag
    46861 tagtagcaac agcaattgga tggacagcta cttatcttgg cctagtcgct ccggtatcgc
    46921 agcaagtcac atgggtagta ccgccgttct tattgtcatt cttagcaaca ggagcggatt
    46981 ggcgggcacc gatcgtaaca ttagtatgta tggtcgtgac tttcttgatt tgggcaccat
    47041 ttgtcatctc tgcaaacaaa atggaccctg ccgcaggcga acaacaataa gatgaacgtg
    47101 aggagcggga cgctttaagt ttcgttcctc ataatgaatt ttcgaggagg aactgaaatg
    47161 attacaattg ccagtgcaat gcaacaagat gaaatcaaag aattgttaga aggatacaat
    47221 gaagaaggaa tgacttttac atttaaagaa aaacaaggaa tcaagctgtt ctttgaaaca
    47281 agtgcagaag atccggaagt tgcggctaaa aaagcaaagg aactaatcaa agcacagcct
    47341 tggggaacag tcctttattt tcaatcaaca gctgtttaga aaatggttta aaaagatagg
    47401 atggttgaga tgataaaaaa cggtttattt ttatgtagta ttggttcttt gatcgtgttg
    47461 tatagtttga atccacattc gcaaaggtat gatttgtata gtatggtcac aggtcttttt
    47521 ctaacaggat taggaatcta tttcttcatg aaggggaaaa aaagagaaga agcaaaggag
    47581 aaaaataact aatggaaatc acgaaaaaat taaatattcc agcatcgttt ttttacgata
    47641 aagttatgga ctctgtcctt ttcgatgtac gtaaagcgac cggcaaatca gtaactagaa
    47701 aacagctgaa aaatttggag tacgtcaaac aattctcgca aaataaccgg gctcgcataa
    47761 aaatcgaaga agtcattgaa aaccagtctt ataagtttcg tacatcgaca acgaagaatg
    47821 aattcgtcgt tcattatcaa atcaaaccat tggacgattc gacctgtgag gttcattaca
    47881 cagaaaaaat ggagtcttat ggcatgcttc aaaagttgaa tgatatgctt ttaggaacaa
    47941 tcttgatgta ttttaaacgg agacgtttca aaaaaatgct agatatgatc gaagcaacgt
    48001 attaaatcaa tgaaacatgg tactatttag ttattgaaaa atgaagtaag ctagaaggaa
    48061 tttttcatag gaagatagat ctttcctgcg aatcctcact agcttttttg taggaagggg
    48121 cagcaaaatg acaaacaaag taagagagct aggtgtatct tcgattgagg aaatgtttga
    48181 tttagccact tatgcattta atggggcaga tacacctgaa aggcgagaac gttttcgtac
    48241 acttgcggag aattcttgga attacggatt ttttgatgag gaggatcggc taacaagtca
    48301 agtcatggcg accccttttc cagtgaattt ttacggacaa gagtacctaa tggccggcat
    48361 tggctatgtt gcttcttatc cagaagcaag gggacaaggc gggatcaatc agatcatgga
    48421 aaagatcctt gaagattgcc gtgatcgtaa agtaagctta tcttatctgg cgccattctc
    48481 ctatcccttc tatcggcgct atggctatga acaatcattt gataaaatca gttatcaact
    48541 aaacagtcgt gatgtgccgt ttatcaaaaa aaaatctgga aaaataaagc gtatggattt
    48601 tgaagatgca aaagctgcaa tcagaagaat ttatgaacag atgccggaaa accaacgagc
    48661 tggtctgaag cgcgaagact ggtggtatac gtataaattc aagcaaaaag gcaattacca
    48721 gtttgctttg cattttgatg ataatgagga agcaacggga tatatcgttt atcagcttgc
    48781 aacgtcttcc tttattattg cggagtgggg atttttggat catgatgctt tttgcagcct
    48841 tgttcgcttc gtaagttctc acaatggtgc ttttgaaaca tttgaatatt catgcggcta
    48901 cggcggtaat gatcaaaatt atctattaga aaatccattt gcttctgtaa tgatcacacc
    48961 ttatatgatg gcgaggatcg tagatatttc tttgtttttg gaaaattatc catttaaaaa
    49021 gagacaagct gcatttgccc tacaaataga agaagatcat tatgcgaaat ggaatgaagg
    49081 aatcgtggaa gtaacaatcg aaaatggaaa aaatcatctc tcaaaagtag aacaaacaaa
    49141 actcccaatc gttcgaggaa gtatccaaca tatcacgcag ctattattag ggtatcgtaa
    49201 agtggaagag ttggtattcc aagaaagaat acaggtgaca aaagaatatg aagaaacgtt
    49261 aagctcaatc ttgccacagc aacagccaat tcttaatgac tatttttgat aaaaaacggg
    49321 tgatgacaat atttttgtca ccaccctttt gtttttcatt tggcatttga aggaaaatgt
    49381 taaagtaaat cctctttttt cgtaaatttg aattttttgt tttcttttgt tttagggttt
    49441 tcttttttgt ggtcttggat tgcttctttc gtaatctttt cccgattttt tttagatgaa
    49501 tctaagcgtt ctgcgtatac tttttctttt ggcttatcat tcattggaaa ggacctccct
    49561 acattatttc attctacctc tagtttatca tctgttaagg actagtggaa acgttttgcc
    49621 taacttataa gctgattttt ttagcagata agcttgcaag taaaaaacaa acaagctaca
    49681 attgatttat tgaaattgtt cctgtagctc agtaggatag agcaatcgcc tcctaagcga
    49741 taggtcgctg gttcaattcc agtcaggaac gtacaaataa cccctgtatc ctttgtggta
    49801 caggggtttg ttttgtattt gtgccaaaaa tgtgccaaac tagttatcat ttttatataa
    49861 actatcgaac acagtattga ttttttcttt atcctgatct tcaagctctc gaacaatatg
    49921 aagataagta gacattgtag tttctaaact gctatgtcct aaacgtttcg atacgctaag
    49981 tatatttact ccttgataaa gtaaaataga tgcatgtgta tgccttagtc catgcaaagt
    50041 aaattgcttt tcaatcccta attcttttaa ttttttgcgc aaatatttgc tgacggcgtt
    50101 tgatgaaact aatccatttt ttaagttgaa aaaaacaaaa ttatgagggt ttttaacttc
    50161 aaaattctcg aataattctt tttgattgat tttaaacttt tttaagagat caatcgtatg
    50221 tttatcaatg gaaattttcc tatttgaagt ttcgtttttt gtttttcccc attcatttaa
    50281 tttataattc catgttttat taatagtgat gatttgatct tcgaaatcga tatcttccca
    50341 tgttaatcct agaagttcag caaatcgcat tccagtagca ccagcaacta acacgagcat
    50401 aggagaagag tactgcgcag aaaggttttc ttctacgagt ttcattaaag atttaaattg
    50461 atcataatct aaatatttat cttcttttgg cttcaatgaa tcttttcctt tgattactgc
    50521 ttttctagtt ggatcaaacg gaattaatcc ttcttctacg gcatctttca acgatgctct
    50581 aatatggtta ttaaacttaa taacggtgga ttttacatgg tctttcgcat acttatttaa
    50641 aaagcgttga tatccaactc tatctaaatc agaaatcaat accgctggca tatatttctt
    50701 tatgttcatt aacgtatctt cgtattttct ataagtgata ggagatactg ttccttcttt
    50761 ataaagttgc atccaatcct caaagtaatc tgataataat agatttttgc gatccccttt
    50821 aaggcctttg ttcaagttat attctaattc gttggcggca tcttttgctt cgccttttgt
    50881 cttaaatcct gattttctta tcttcgtgta ttttccgtca tcttttttgt atgaaatttc
    50941 gtattgccaa ctatttccac gtttgactaa tcttgccata attgaatcta ctctctttct
    51001 ctgatacaat aggcactata aagaagccta ctgtataggt ttgtttttct tagaacacgc
    51061 ttgctttgga cggtgggcgt gttttttatt tattatacag ttgctttaaa aatagctgct
    51121 ttttcaaatg atactaaagg agagaagtgg atttctattt ctcccttttg gtttaacgtg
    51181 aaatgagcag ttacatccat ttgttttcct ggagcaacag atcccattgt attttcgttg
    51241 gcatatgtct ctgatttttt gtcatctggt ccatatactt ccacatctgt acctacaggg
    51301 atatctgaat cgccatcatt ttttacggta taagtaattt ttactacttg tgcaggttgg
    51361 ttttcttcaa attgatttct ttcatcagtt agttctacac tatttagtgt atattcagca
    51421 tcaccaactt ttacagtgtc accaatctta tagaaggtat cgcttttttc ttctttagaa
    51481 gatgaagcag aggttgaaga tttagttact ttttcgccac cattatcact tgctttatta
    51541 ctatctgatc caccatttaa agcagaacca ataattataa ttaaaattac tgctaatacc
    51601 caaaaccata cttttttgta aaaaggttta ctaactttat acatttttcc gtcttgaccc
    51661 ataacttttt ttgccattta aatattcctc acttcttgtt ataatatatt tgcgatctca
    51721 gaaatgaggt atgagtccgt gtgccagcac gggctttttt aatgttttgc tgaaatcggt
    51781 ttttttaata attcttgatg tattcataca tattgccttg cgtaagaata ttttttctta
    51841 aaatagcatt ggcagacagc ataacaagtg tatctgagct tattctgaat agaataatat
    51901 tccataaatt tttcgaggtt aaactgagat tcatcagtca gttcgttctc aatatagata
    51961 tttaataaaa ttagaatagc tattttatct gcttctgttt caaattttga gtgaaaagtt
    52021 gtagaagtat cgtacaaaac tgaaaattca aaatgagagg cgctgaaatg tgcaagttca
    52081 tgagataaat gaaaggcttc tgcagtttcg ccatatagat tttcattcaa aaaaatgatt
    52141 ctaggcttcg gataatagaa gccaggctct ttcatttcca tgtaaactac tttcaaattg
    52201 tattcgctca acatttcttt caatttcaaa tacatacaac ccatcactcc aactattcat
    52261 tttcctctaa agctttagca attgcaatcg ctttacgcat tgtctcctta gatatttctt
    52321 ttccatcaaa agaaaaaaca gtatcgtttt ctgataaatc cacatgttta ggggtttctt
    52381 tttcttcgcg ccccagaaga tagtctacag agacatcgaa atagtcagca atttcttgca
    52441 aacgttctgt agaggggttt gagttcttaa gtctatataa gacgttttta ccatagccta
    52501 ggtcttcttc aactttattt aatgattttc cctgtttttt cgctaactct tttattctct
    52561 caaacgttat catatcaata ttcctttcgt tcaagaaaaa atatttaact ttatttgtta
    52621 aaattcgttg actattttaa caaaaggtgt taatatcatt cttgtaaaca agtttaacaa
    52681 ctaaaaagac aacaaaaaac actattgatt aataaatgcc aaccgccaag aaagctttta
    52741 aaatcaatgt ttatatgtct tatttaacta tgttctgatt ttaacactgt atgttaattt
    52801 agtcaacgca gttttttaaa aagttgttaa atttgtttac gaatataaaa gaaaggagag
    52861 aagaatatgg aaaaaacagt ctcggcaaaa atcgaagatt tgaaattaga catactgaaa
    52921 caagcaaaag tggcgatgga acacgcggta gataaagaag actctgccat ggttgcagcc
    52981 atagcagaga ttttagctca cgtttagtca tcgtttttac ggtcttctgg aagctgacca
    53041 tagataacag cataatgaat atctaaataa gcttgaacaa tttcttttgg cgaaattgat
    53101 tcgcctttag tagatacttg tgattcgtga tttagccatg caacaactaa atccgcagcg
    53161 atttcaaacg gcgttacact ttgtgtcagt tgccataaag aatttcacaa tatgtatggt
    53221 cgtggtaata ataccaaaga gcaatttgag gaatttatgg aaattaaaaa atacaaaaaa
    53281 tcatttatta aaggaggcta aacaatgaac aaggaaatga gccaaacaat caaggtacaa
    53341 aaaatgatag atgatctaac acatggaatt gatagccaag ccgataaaat catcaaggaa
    53401 ttgcaaggac aaaaagttaa ggatgcaaaa atgcttctca agactatcaa ctttgaaatg
    53461 aatccaacaa aaagaaagct cgccgatgtg ttggaagaaa agttggcttc cgctatcaac
    53521 gagcaagaat tactatttga aaccgatact tttaacagct gatgttttat agatgacagt
    53581 ttcatccgaa tctatatcat cgccgagaga aaagaatgtt gcttttgaca aaaagccgat
    53641 aagacctaca agcggtcttg atgttgcaag agtagatacc ccaatatcac cttgcaaggt
    53701 attcacgtaa atttgagaag aatagaacat tccatctttt tcttcgatat ttgacgcatc
    53761 atcgtatcca acgaccaaag tatcggcaaa tattttgatt ttttctccat caagcatagt
    53821 aattacaaat tctggtttca taaattttca cctccttaac aattatttca gcctgtcaca
    53881 ctgataagga aattatacca aagaaaggaa tgagaaatat gaatacacca caaattttta
    53941 atttcgagca aaacgaagtt cgaactattt tagtaaatga tgaaccatat tttgtaggaa
    54001 aagatgtagc agacgttcta ggatattcga atccgcaaaa agctattcga gatcatgtgg
    54061 acttggagga taagacgcag aacgattcgt tcaccgtcaa tggaacagca gttgttttga
    54121 ttaacgagtc aggcctttac agtttaatct taaaatcaaa acttccctct gccaaaaaat
    54181 ttaaacgttg ggtaacaagt gaagtgttgc cagcaattag aaaacatgga ggttatctaa
    54241 ctccagaaaa agtagaagaa gctttgctta atccagatac aatcattcaa ttagcaactc
    54301 aactaaaaga agaaagaact ggaagattaa tcgcagaaca aaagattgca gagtacgaac
    54361 ctaaaatctc ctatttagat agcatattat cttctacaga ttcagtaaca attagtcaga
    54421 ttgcagcaga ttatgggatg tctccacaac agatgaataa attacttcat aaactaggta
    54481 ttcagaaaaa agtcggtaac caatggttat tgtgcaaaaa acacatgaac caaggataca
    54541 caaaatctca tacaactgag atcccgaaag ccgatggtgg cactaaaatt gtaatgaata
    54601 ccaaatggac acagaaaggg cgtctattta tctacgaatt actaaaaaaa gaaggatatt
    54661 accctcaaat ggatttagag gaaattggtt agaaaggagt tttagtatga ctgacattgc
    54721 agaaatcact caacgagata gagaaaaaat caaagaatat gtcgaaagtt caaagttctt
    54781 aacttacacc atgctcgctg aaagattcgg cattagcaaa agctacttat ctttaatttt
    54841 aaacggtaaa aagacttctg cagaagcaaa tagaatcata gattcgatta ttactatgta
    54901 cgaattgtaa agggaggaac agctaatgca atatctagaa gcgaaaatcc cagttccaga
    54961 aggctatgta attatctccc aagtggatta tgaggagtta aaaaaagctg atgatactgg
    55021 tagatggatg acgttgccag aagtgctaga acggattaac agaaaatatg attggtttac
    55081 ttctagagtt ttaaagaacc caagatatag aaatattatc gatatcgaaa aaaacaaaaa
    55141 tggatttgtc tattatccag ttgaaggaag agacacatat ctatttttaa gaagtaaaac
    55201 acttgaattt ttagaaacaa atttttcgga aatcttaagg aggtaagcgg atgggtaaat
    55261 ttaatagagc actagtattc agcgcaccac taattatcta cgctttaggt ctttggggaa
    55321 gcagacaagc attaatcggc acaattgtat atatggtctg gatctttatg gggcttgatg
    55381 aagctgaata tagagcgaaa aagccagtcg ggagggactg acttaaccta attaattaaa
    55441 aattgtaaca aataacgcaa aaataaagct aatgaaattg tattgttagg atctactcca
    55501 atagttatat ttacaatttc ggttactact agtcctatca aagctgcccc agtttgctct
    55561 aaataccaca ttttatcttt gtagaaagca ggtgaagctt gttgtttttc agatggttca
    55621 ttagttatat ctttgttctt ggctgtattt atttcttcaa aaagctttat agaatcatca
    55681 aggactttcc tttgctcatt aaggatatca aaaaacttag cctcaaactc atcagatgga
    55741 ttagaagtgt ctttaaaaag tagatttttt gtactaactt ccggttcgga agaaaatgct
    55801 ttttctaatt cttgggtagc tacccctatt ctgaagtcgt acttagacat gtcaacggca
    55861 aatctttctg tgattttaga gatatcagaa aaataaggtt ttggcatagt agctgccatt
    55921 tttttagcaa tattaaaact tggagattct aattgtttct gtattgcgaa caaagaagga
    55981 ggaatcatat tagcaataga tttgttacgt ttctcgacaa tttgaatgag agaatcattg
    56041 agtattgctt gcgaagctat aaaagtatct tgaattgcaa ttaatttttt agcttgttct
    56101 atcgcaggct gcatagcgtt aacgacatct agagaaggag caatggtttc accgattaat
    56161 tttgaagaag ctatcaattt ttgataataa ggagtatctg aaatatccaa ttatatacac
    56221 caccttttta cctaaattat accaaaaggg agagaaaaaa atgcaagaat tagtaatttt
    56281 gaaaaataaa gaagctgtga ctacgagctt acaagtcgca gaaacattcc gaaaggaaca
    56341 tagagcagtt ttaaaggcga ttgacgatct aaaagatggg cttgcacaaa agtatgcaga
    56401 cctattttgg gaagatgtgt acatccatcc gcaaaacaaa caggcttatc gaattgtcta
    56461 tatgaatcgt gatggcttta ctcttgctgt attaggattc aacaatactc aaaaagtttt
    56521 agagttcaaa ttgaaataca ttgaagcgtt caacgaaatg gaaaaggcaa ttcgcaaaaa
    56581 tacagctttt gacacaattg aagacatgat gatctatcaa ttagaagaaa tgaaagatgt
    56641 taaaaaagat gtttccatgc ttaaaaatac tatgcgaatt agcggacaac aagagtttga
    56701 aattaagcaa aaaggaaata cgaaagttat ggaagttcta gggggaaaag aaagccgagc
    56761 ttatgaagaa atcagcaaaa aagtattctc aaaattttgg tctgaattta aacgtacctt
    56821 ttcaatccca agatatggcg agttacctcg taagagattc gatgatgctg tttcatttat
    56881 tgaaatgtgg ttaccagaaa ctgcgattcg catggaaatc gatcaactga acagacaaca
    56941 aagacttttt ggtgatgaaa atgaatagag ctgaagcgct aagaatcggg acggtaattg
    57001 ctaatcgctg gtggagacac aataaaccaa tcatcctaag ccaacaacat attgacaag
//
LOCUS       DACDIG010000009        51503 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.009, whole genome shotgun sequence.
ACCESSION   DACDIG010000009 DACDIG010000000
VERSION     DACDIG010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.009"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(231..1958)
                     /locus_tag="IV247_000619"
     CDS             complement(231..1958)
                     /locus_tag="IV247_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8776712.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(1951..3144)
                     /locus_tag="IV247_000620"
     CDS             complement(1951..3144)
                     /locus_tag="IV247_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8776713.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3331..3975)
                     /locus_tag="IV247_000621"
     CDS             complement(3331..3975)
                     /locus_tag="IV247_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8776714.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4069..4203)
                     /locus_tag="IV247_000622"
     CDS             complement(4069..4203)
                     /locus_tag="IV247_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAP8776715.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4205..6337)
                     /gene="feoB"
                     /locus_tag="IV247_000623"
     CDS             complement(4205..6337)
                     /gene="feoB"
                     /locus_tag="IV247_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAP8776716.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6334..6807)
                     /locus_tag="IV247_000624"
     CDS             complement(6334..6807)
                     /locus_tag="IV247_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAP8776717.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7208..7432
                     /gene="nrdH"
                     /locus_tag="IV247_000625"
     CDS             7208..7432
                     /gene="nrdH"
                     /locus_tag="IV247_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAP8776718.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            7591..9750
                     /gene="nrdE"
                     /locus_tag="IV247_000626"
     CDS             7591..9750
                     /gene="nrdE"
                     /locus_tag="IV247_000626"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAP8776719.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            9800..10765
                     /gene="nrdF"
                     /locus_tag="IV247_000627"
     CDS             9800..10765
                     /gene="nrdF"
                     /locus_tag="IV247_000627"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAP8776720.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(10854..11993)
                     /locus_tag="IV247_000628"
     CDS             complement(10854..11993)
                     /locus_tag="IV247_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8776721.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12302..13015)
                     /locus_tag="IV247_000629"
     CDS             complement(12302..13015)
                     /locus_tag="IV247_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAP8776722.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13269..13970)
                     /gene="nagB"
                     /locus_tag="IV247_000630"
     CDS             complement(13269..13970)
                     /gene="nagB"
                     /locus_tag="IV247_000630"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAP8776723.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14204..15736
                     /locus_tag="IV247_000631"
     CDS             14204..15736
                     /locus_tag="IV247_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8776724.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(15927..17129)
                     /locus_tag="IV247_000632"
     CDS             complement(15927..17129)
                     /locus_tag="IV247_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8776725.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(17211..17900)
                     /locus_tag="IV247_000633"
     CDS             complement(17211..17900)
                     /locus_tag="IV247_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP8776726.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(18006..18374)
                     /gene="rplL"
                     /locus_tag="IV247_000634"
     CDS             complement(18006..18374)
                     /gene="rplL"
                     /locus_tag="IV247_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAP8776727.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(18427..18930)
                     /locus_tag="IV247_000635"
     CDS             complement(18427..18930)
                     /locus_tag="IV247_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAP8776728.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(19340..20029)
                     /gene="rplA"
                     /locus_tag="IV247_000636"
     CDS             complement(19340..20029)
                     /gene="rplA"
                     /locus_tag="IV247_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAP8776729.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(20133..20555)
                     /gene="rplK"
                     /locus_tag="IV247_000637"
     CDS             complement(20133..20555)
                     /gene="rplK"
                     /locus_tag="IV247_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAP8776730.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            20898..21566
                     /gene="sdaAB"
                     /locus_tag="IV247_000638"
     CDS             20898..21566
                     /gene="sdaAB"
                     /locus_tag="IV247_000638"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAP8776731.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            21579..22451
                     /gene="sdaAA"
                     /locus_tag="IV247_000639"
     CDS             21579..22451
                     /gene="sdaAA"
                     /locus_tag="IV247_000639"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAP8776732.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(22542..23561)
                     /locus_tag="IV247_000640"
     CDS             complement(22542..23561)
                     /locus_tag="IV247_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP8776733.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(23734..24621)
                     /locus_tag="IV247_000641"
     CDS             complement(23734..24621)
                     /locus_tag="IV247_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP8776734.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(24698..25243)
                     /gene="nusG"
                     /locus_tag="IV247_000642"
     CDS             complement(24698..25243)
                     /gene="nusG"
                     /locus_tag="IV247_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAP8776735.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(25344..25514)
                     /gene="secE"
                     /locus_tag="IV247_000643"
     CDS             complement(25344..25514)
                     /gene="secE"
                     /locus_tag="IV247_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAP8776736.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(25538..25690)
                     /gene="rpmG"
                     /locus_tag="IV247_000644"
     CDS             complement(25538..25690)
                     /gene="rpmG"
                     /locus_tag="IV247_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP8776737.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            25852..26496
                     /locus_tag="IV247_000645"
     CDS             25852..26496
                     /locus_tag="IV247_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP8776738.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(26552..27055)
                     /locus_tag="IV247_000646"
     CDS             complement(26552..27055)
                     /locus_tag="IV247_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP8776739.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(27068..27114)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            27652..28617
                     /locus_tag="IV247_000647"
     CDS             27652..28617
                     /locus_tag="IV247_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP8776740.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(28716..29888)
                     /locus_tag="IV247_000648"
     CDS             complement(28716..29888)
                     /locus_tag="IV247_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8776741.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(29875..31020)
                     /locus_tag="IV247_000649"
     CDS             complement(29875..31020)
                     /locus_tag="IV247_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8776742.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(31034..31958)
                     /locus_tag="IV247_000650"
                     /pseudo
     CDS             complement(31034..31958)
                     /locus_tag="IV247_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            32075..33154
                     /locus_tag="IV247_000651"
     CDS             32075..33154
                     /locus_tag="IV247_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP8776743.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            33186..33779
                     /locus_tag="IV247_000652"
     CDS             33186..33779
                     /locus_tag="IV247_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8776744.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(33889..34116)
                     /locus_tag="IV247_000653"
     CDS             complement(33889..34116)
                     /locus_tag="IV247_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8776745.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(34219..36807)
                     /locus_tag="IV247_000654"
     CDS             complement(34219..36807)
                     /locus_tag="IV247_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAP8776746.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(36827..37486)
                     /locus_tag="IV247_000655"
     CDS             complement(36827..37486)
                     /locus_tag="IV247_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP8776747.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(37535..38038)
                     /gene="trmL"
                     /locus_tag="IV247_000656"
     CDS             complement(37535..38038)
                     /gene="trmL"
                     /locus_tag="IV247_000656"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAP8776748.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(38259..39125)
                     /locus_tag="IV247_000657"
     CDS             complement(38259..39125)
                     /locus_tag="IV247_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP8776749.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            39234..39875
                     /locus_tag="IV247_000658"
     CDS             39234..39875
                     /locus_tag="IV247_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAP8776750.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(39937..41298)
                     /gene="mgtE"
                     /locus_tag="IV247_000659"
     CDS             complement(39937..41298)
                     /gene="mgtE"
                     /locus_tag="IV247_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAP8776751.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(41316..42215)
                     /locus_tag="IV247_000660"
     CDS             complement(41316..42215)
                     /locus_tag="IV247_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP8776752.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(42217..43014)
                     /locus_tag="IV247_000661"
     CDS             complement(42217..43014)
                     /locus_tag="IV247_000661"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAP8776753.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(42992..43696)
                     /locus_tag="IV247_000662"
     CDS             complement(42992..43696)
                     /locus_tag="IV247_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAP8776754.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            43827..44402
                     /locus_tag="IV247_000663"
     CDS             43827..44402
                     /locus_tag="IV247_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAP8776755.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            44520..45197
                     /locus_tag="IV247_000664"
     CDS             44520..45197
                     /locus_tag="IV247_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP8776756.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(45276..47084)
                     /gene="pepF"
                     /locus_tag="IV247_000665"
     CDS             complement(45276..47084)
                     /gene="pepF"
                     /locus_tag="IV247_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAP8776757.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSDAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(47260..48258)
                     /locus_tag="IV247_000666"
     CDS             complement(47260..48258)
                     /locus_tag="IV247_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAP8776758.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(48484..49137)
                     /locus_tag="IV247_000667"
     CDS             complement(48484..49137)
                     /locus_tag="IV247_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAP8776759.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            49571..50866
                     /locus_tag="IV247_000668"
     CDS             49571..50866
                     /locus_tag="IV247_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP8776760.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
     gene            complement(50980..51378)
                     /gene="spxA"
                     /locus_tag="IV247_000669"
     CDS             complement(50980..51378)
                     /gene="spxA"
                     /locus_tag="IV247_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAP8776761.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT        15410 a        10800 c         9085 g        16208 t
ORIGIN      
        1 gcacttcgta cataataaac tttaatcaca ttagtctttc atacctacga tcgatctctt
       61 tgactcgcac ccataaattc ataatagttt gtcggcgtct ttggacgttt cgaagatgag
      121 ctacgcattc tcttttttta tagacaaaaa agatagagac gaacatggag cttccaaatt
      181 tggatatttg atccaatttt ggaagctcca tttatttcgt gtctgtcttt ttattttttc
      241 cagctttcga ctaattcttg gttctctttg atccagtctt ttgctgcttg ctgtggatct
      301 tttccattgt tgatttccaa cataactttt tccatatctt tttctgacca atggaaattg
      361 tctaatactt tgtaagcttc tggctggtct tctttcaatc cttttcgaac catcgtatgg
      421 atctgctctt cgcttcccat tccgttttca ggatcttcta agtatttcag atcatatttg
      481 gcaaacatcc aatgaggtgt ccatcctgta actacaattg gttcgtgttt tttgatcgct
      541 tgacctagtg caacggtcat tgctcctgaa gaagatgtct ctactttcca gtctttcaag
      601 ttgtcatatt tttggatcgt gttttcagca gccgtaacta caccagctcc tggctcgatt
      661 cctatgattt tcttacctgc ctgatcggtt aactcatcga tcgaatttac atccatgtag
      721 gctggcactg ctaaaccaac ttttgctcct gtcaaattgg cacctaggtc ttcgacttga
      781 tctttatatt gtgcatattg tgaaccatga gttttaggta gccaagctga aaccatggca
      841 tctgactcgc catttgaaac agatttccac atgattgaat tatctaatgg cgtcatcgtt
      901 acatcatagc ccatttgttt caatacttca ccgactacat ttgttgaagc aacttctgta
      961 tcccattcaa cataagataa gttgatacgt tttgtttctt tggcagatga aaaactccct
     1021 ataccaatcg cacctaagat cactactact acagcaccaa tgatcagtcc ttgacgtttt
     1081 ttacttttct gtgttgttgc tcctgcagct tttttattgt ttggctggtt caaatgctgt
     1141 gtaaaacggt caatgatgat tgctaagata actaatgcaa ccccgttgac aaatccattc
     1201 ccgacttgtg ctctttgcaa tgctgataat acgccgcgtc ctaagccggg tgcaccgatc
     1261 atagaggcaa taacgaccat tgaaagtgac agcattgttg tttgattgat ccctgccatg
     1321 atcgtacttt tagctaaagg caattctagt ttgaatagtt tttgacgact tgtgctcccg
     1381 aatgaatcag aagcttctac taattcttta ggaacttgtc gaatccctaa attcgtaaaa
     1441 cgaacggttg gtggcagagc aaagatgact gaggcaaata cccctggaac catcccgata
     1501 ccgaaaaatg caactgctgg aatcaaataa acgaatcctg gcattgtctg cataaagtca
     1561 aggatcggtt tgataatact ttgggcttta ctgctttttg ccatcaagat ccctaatggt
     1621 accccaatga tgatcgagat cacactagat aaaagaacaa gtgtgacagt attcatcaaa
     1681 tcattccata gaccttggtt ataaataaac catagaccga ttaatgtgaa caacgtaagt
     1741 cctggacgtt tcttagaaat aaagaaagct gctaccgtca ataacaagat aaacaaagga
     1801 gctggaatca ctaacagtaa atttgtaatc cccgacatca ctgcctgtcc aatcgtttgt
     1861 aaaaatgaaa ataggccaga aaaagttgtc gtaaaccatt ctgtgatatt ttctacccag
     1921 tttgccactg gtaattgata gttcaataga ttattcatgt tcactcacct cgcttgtttc
     1981 tgctaatgct tcaatcacac tacctcggat gacgacacct tttaattttc catcctctac
     2041 aactgcaagt ggagtcggtg agtcataaat aatattgaaa atatcactta ccaccatgtc
     2101 tttcccaatc accgtcacat tttcatctag gaagtcgatc aatggacgtt tttgcttcct
     2161 agcttctaac gcttgatctg cagtgatgat ccctttcagg tgtctctttc tgtcaacagc
     2221 catcagcatg ctgacttctt cattacgcat ccttgtcaat gcaacgtttg gtccatcaga
     2281 ttcgatattc gtcgtcaatg caggtaccat gatgttttga gccgtaagca ctttggaacg
     2341 atcgacttct tccacaaatt cacgtacata atcatttgct ggattggtca atatttcttc
     2401 acctgttccg atctgcatga tttcgccgtc tttcatcaag gcgattctgt ccccaatccg
     2461 taaagcttca ttcaaatcat gggtaatgaa aataatcgtt ttttgaacat tggcttgcag
     2521 gtccagtaat tcgtcttgca tttctcgccg gatcagcgga tcaagagccg agaacgcttc
     2581 gtccatcagc aagatctcag gatcatttgc taaagctctg gctaggccaa cccgttgttg
     2641 catcccacca gataactgac taggatattg gtctttaaat gaaagcaaac tagaattttc
     2701 taatgctttt tctgcttttt cttgtcgttc ttctttaggt acaccacgga cttctaaccc
     2761 gtattctgta ttttccaaga tcgttcgatg tggaaacaga ccgaagtttt ggaaaaccat
     2821 attgatttta tgacgacgga cttctcgcaa ttcttctttg cttaacttcg atacatcttc
     2881 tccatcaata tagatactgc ccgaagttgg ctcaatcaga cgattcaata gacggatcaa
     2941 tgtggatttc ccactcccgg aaagccccat gatcacgaag atttctcctt cttttacgtc
     3001 aaaattcacg tcatacacac caaccgttgc accggttttc tttaaaatct ccgttttcgg
     3061 ctggtgatca ttcatcatat ctaaagcggc ttgtgtcttt ttaccaaata tctttgttaa
     3121 atgttttact tctactttta ccaaacaaat tctccttttt tatgtaaatt catttttctt
     3181 tctgaaaaaa gtgaccacac tatgttaaca tcatgtagtc actttgtcaa aaatgaaaga
     3241 aatttgcttg tccaaactga aaaaaacttg gctaaatctt tgtaaataaa aggtttagcc
     3301 aagttaatat ttttttaata tttcacacaa tcattctgca agctgatgct ccgggtaaaa
     3361 aaagtttgtc atacgctgta gtgtatattc atttccaata aaatgaacga tgtcccctgc
     3421 cgtaaattta gcataggggc caggtgaaac aagcagttct tcattatgag aaattgcgat
     3481 aactgtggcg ccagtcgcct gccagatatt caactcgcta attgaacggt ccagatgcat
     3541 tgctttttct gataaagata attcaaacgg aagtaaagga ttcatcttat gtattcgctt
     3601 cgtttgattg accagctgat ccaataattg agaaaaatga ttcagctcat cttgttgctt
     3661 ctccacactt gccatgattt ctgcttttgt atcttggatg gattgtacat cttgatattg
     3721 caataggaaa tcttttgctt tttccttcga agcaacaaat gccccacttc catgccgtac
     3781 ttccatgatt ccaagatcta ccagcacatt gatagccttg cgggtagtct caggagaagc
     3841 attgtacgta ctggcaagtg ttgagcgggc atggatcttc tcacccactt tatatcggtt
     3901 ttcaacgata cgttcggcta aatcaacagc aatctgctgg tagcgcgggc ggacgatttt
     3961 tttctttcca gacattttct ttcctctttc tttttttact ttccctcatc ttactatgct
     4021 ctagcaaaat aagcaaaaaa ggggagaata gcctcttgca acgactgatc atcgcttgat
     4081 ctgttccttt tttactgggc aagctgtttg acagtcatca caattcttac cggattttaa
     4141 acgagtatag acaattgcgc ctgcacttcc aaagattaac acacttaata agaatgtcgc
     4201 catcctattc ctctccttct aatgacaata tagtataaaa ctggttcttg gattttggct
     4261 gacggaacaa aacataaacg agtcctatca tcagtaaaat agcaagatac gtccctaacg
     4321 ctacctgtcc accctccaat aagtgtccca actgataaat cacaaaacta actaaataag
     4381 ctaaaccgca ttgatagcct atggcgatcc aagtccattt cttggcattc atttcccgat
     4441 ggatagcccc aattgccgca aaacaaggcg cacaaagtaa attgaaggct aacaatgaat
     4501 aagctgctgc gggagtatat gtgtgttgta gcacttgcca gatttctctg ccattttcgg
     4561 atacttccgc atgaccaaac aaaataccaa atgtgccaat aatattttct ttagcgacca
     4621 atcctgtaat cgctgcgact gttccttgcc agtttcccca accaagcggc tgaaaaacaa
     4681 ctgccaggat tcgcccgaaa aaggctaaga tactttggtc ttcagaaaca ggttgagcat
     4741 aaaaattata agaagagctg aaccagatca cgatactaga aacaaagata atcgttcctg
     4801 cttttttaac aaaagacttg ctgcggtcat aggtttgtct taggaccgct gaccattgag
     4861 gaaaatgata tgcgggcaat tccatgataa aaggagctgt atctccagca aacaaacgtg
     4921 tttttttcaa gctgattcca gagaaaataa ttgcagtcat accaagaaaa taggcagaag
     4981 gagctaccca agattgatga ggaaaaaagg cccctgctac taaggcaata atcggcaact
     5041 ttgcagaaca aggcatgaag gttgtgacca tgatcgtcaa acgacgatct tgctcgtttt
     5101 caatcgtgcg actggccatg acgcctggca ctccacatcc tgtagcaatc agcataggaa
     5161 tgaaggattt tcctgaaaga ccgaatttcc tgaaaagtcg atccatgaca aaagcgatcc
     5221 tcgccatata tccgcagtct tccagaaagc tcaagcataa aaacaatata atcaattgcg
     5281 gtagaaatcc taacactgct cccacgcctg cgatgatgcc gttcaagatc agctcctgca
     5341 tccatcccgc tacttgccat tcagctaaca atctgccaac atgttctggt aaccatgtac
     5401 caaacaatgt gtcattgatc caatctgtcc ccattgttcc tactgtttga atagccagat
     5461 aatagatcag ccacatcaca aatgcgaaga tcggcaaggc taaccaacga ttcgttgcaa
     5521 tctgatcgat tttgtcactc atcgataatt ggaaagaatc atttgacaca acacagagtg
     5581 tacatagttg ggtaataaat tcataacgtt cattgaccag aatcgtctca ctatcgtcgt
     5641 ccaatagttt ctctgttaac tggatcagtt gctcgatctc tttttgctga gaaactgcta
     5701 tctcgtaatc ctctcttacc ttctgatctc gttcaaaata tttcattgca taccagcgtt
     5761 tcttgccgct ttgttctcct agtacatctt caatttcatg taaagctgct tctaaacgtg
     5821 gatcatatgt acagggaaca ggaatagaag gccgcttttt tgcagactcc tgaatctttt
     5881 cgattagttt ttcgattcct tgctttttca atacgctgat tggtaaaact ggtacaccaa
     5941 gaccatagga aagcttttct agattgattt tttttccttc ttttttgatg acatccatca
     6001 tattcagacc aatgatcata ggaatgtcta aatccaataa ctgcgtagtt aaataaagat
     6061 ttcgttctaa attggtagca tcaataatgt tgatgatcag ttcaggctgt tcctgaacca
     6121 aataatctct tgcaatcact tcttctggtg tataaggaga caaagaatag atgccgggta
     6181 ggtcctgaat atgaatgttc ggattttttt tcaacgatcc ttcttttttt tctactgtta
     6241 cgcccggcca attccctaca tattgattcg aaccagtcaa gagattgaac aagctagttt
     6301 tcccgctgtt tggatttccg actaacgcga tattcattct ttttcctccc tttgtatcaa
     6361 gattagttcg gcctctgact ttcgtaacgt cagttcatac cctcgcactg tcagttcgat
     6421 tggatctccc agaggggcta gttttcggat aactattttt acatttcgcg tcaatcccat
     6481 atccattaac cgtcgcttca ctgcgccttg tccgtggatt gctacgacga tcccagtctc
     6541 tcctgctttt aattgatcaa gagaagtcca gctttcagag gaagcagtct ttttattgac
     6601 aaagatttga cctagtattt cccgacttac tgccactcga tgacttttta ccagtaaaat
     6661 ggcattgttg cctgataaat tgacgacgac gattttactt ccggcaacga tcccgatatt
     6721 ttgcaaatgg cgttttgttt tttcttctgt ttcgattcgt tcgacttgat aaacgaaatt
     6781 ctgctttgcc tgcgctaagg tctgcatatt gattcctctc ttgtgaaata aatcatgtat
     6841 taaaatcatt cattatatgt tctaattttc ttacgtgtta cgcaaataaa tgagaatcat
     6901 tctcacatag aatactctcg gtaattgaga atgtcaatta tacttgtgat attatgagcg
     6961 aatctttagt attgatagat aaaaatgaga aaaaaatatt tcttcatagt tttccacaat
     7021 tttttctcta ttaaatttgc tattttccca cacgatactt gttttcagct cttttttcaa
     7081 aaacacaaaa cttagtattc cacagtttcc acaacacaat atgtagtgtt tgttgtttga
     7141 cttttttact aagagtttta tactatctat tatagataat aagaatgtgt tagaggaggc
     7201 actcaccatg aacgtaaaaa tcttttctaa aaataattgt atgcaatgta agatggtcaa
     7261 acggttttta acagaaaatc aaattgcttt tgaagaagtc aatatcgatg aacagcctga
     7321 agcagtagac tggttgaaag cacaaggatt ccaaagtgtt cctgtcatta cttctgatgc
     7381 agaaacagtt atcggattcc gtcctgatca gttgcgtaaa cttgccagct aacatgacta
     7441 aaagaatcaa aacgtgatac atggatttga agggcttgag acagcaccag tttttgtcgc
     7501 aaacttccaa atcctttttt aaagcttaaa aacaatgcaa tgatttgcaa tcaagcatca
     7561 tcaaatacga aatgaaggtt gggcgtacgt atgagtctta aaaatttaca agatgtcagt
     7621 tatttcaagc tgaataatga aatcaatcgg ccagtcaatg gtcaaatccc tttgaataag
     7681 gataaagaag ctttagttgc cttttttgag gaaaatgtca aaccaaatac gatgactttt
     7741 ctatctatta tggaaaaaat caattacttg atcgatcaag aatatataga agcagaattc
     7801 attcaattgt attcaccatc atttattgaa aagctatatc aatttttgga cgagcaacat
     7861 ttttctttca aatcttttat ggctgcttac aaattttatt ctcaatatgc gttgaaaacc
     7921 aacgacggtt ccgcttatct ggaaacttat gaagatcgag tagccttcaa tgcgctttac
     7981 tttgcgaacg gggatgaaga attagcactt gctcttgcag acgaaatgat ccatcaacga
     8041 tatcagccag ctacaccttc tttcttgaat gctggacgta aacgtcgggg cgaattggtg
     8101 tcttgtttct tgactcaagt cactgacgat atgaactcta tcgggcgctc aatcaactct
     8161 gctttgcaat tgtcccgtat tggtggcggc gtagggatta ctttatcaaa tcttcgtgaa
     8221 gccggcgctc ctatcaaagg atatgagggt gctgcaagtg gtgtcgttcc agtcatgaaa
     8281 ttattcgaag atagtttcag ctattctaat caattaggtc agcgtcaagg agctggcgtg
     8341 gtttatttga acgttttcca tccagacatt attgctttcc tttcagcaaa aaaagaaaat
     8401 gcagatgaaa agattcgcgt aaaaactctt tctcttggcg tgatcgtacc tgataaattt
     8461 tatgaattga cacgtaaaaa cgaagatatg tatttattta gtccatatag tgtcgaaaaa
     8521 gaatatggtg tcccattctc ttatgtaaat attacagctg agtatgatca tctagttgca
     8581 aatccaaaca ttcgcaaaca aaaaatcaaa gcgcgtgatc ttgaaaatga aatctcaaaa
     8641 ttacaacaag aatctggcta tccttacgta atcaatattg atacagcgaa ccgcgagaat
     8701 ccaatcgatg gcaagatcat catgagtaat ttatgttcag agattttaca agttcaaacg
     8761 ccttctgtga ttaacggcaa acaggaatat gaagtattag gaacggatat ctcatgtaac
     8821 ttaggctcga caaatatcgt gaatttgatg caaagcccta actttggtca atctgtgcgt
     8881 acgatgactc gtgcattgac atttattaca gatgcatctg atatcgatgt cgtaccaccg
     8941 atccaaaacg gaaataagct gaatcatacg attggattag gtgccatggg cttacacact
     9001 tatcttgcaa aagaacaaat ggaatatggc tcagaggatt cattagactt tacaaatatt
     9061 tacttcatgc tgttgaatta ttggacactt gttgaaagca acaatatcgc tcgtgaaaga
     9121 aaacaagttt tccataattt tgaaaaatca gcttatgctg acggcactta tttcgaaaaa
     9181 tacgtaactg gagaattcca gccaaaatca gaaaaagtca aagcattgtt tgaaggcatt
     9241 tttatcccaa ctgctgaaga ttggaatgct ttgaaacaag cagtaatgaa agatggactg
     9301 tatcatcaaa atcgcttggc agtagcacca aatggctcaa tttcatatat caacgatacg
     9361 agtgcaagta ttcatcctat cactcgcatg atcgaagaac gccaagagaa gaaaatcggt
     9421 aaaatttatt acccagcgcc ttatctatcc aatgagacga ttccgtatta cacttctgct
     9481 tatgatatgg acatgcgtaa agtaatcgat gtttatgcaa tggcacaaca acacgtcgat
     9541 caaggcatga gtttgacttt attcatgcgt tctgaaatcc cagaaggcat ttatgaatgg
     9601 aagacaacaa cgaagcaaac gacacgtgat ttgaatattt taagacatta cgctttccac
     9661 aaaggaatca aatccattta ttacgtacgt acctttactg atgatgcaga agaaatcgga
     9721 agcaatcaat gtgaaagctg cgtgatctaa tatcatagat agattcgctt attcatattt
     9781 taattaaagg agcattctca tgtcagaaac atattatgaa gcaataaact ggaatgaaat
     9841 tgaagatatc attgataaat ccacttggga gaagctgaca gagcaatttt ggctagatac
     9901 acgtatccca ctttcaaatg acctagatga ttggcgtaca ttgtctcaat tggaaaaaga
     9961 tacagtagga catgtatttg gcggtttgac actgctagat actgtccaat ctgaaagtgg
    10021 catggatcag ttgcgtaaag atgtccgcac tccccatgaa gaagccgttt tgaacaatat
    10081 tcaattcatg gaatccgttc atgccaaaag ttattcttct atttttagta cactgaatac
    10141 gaaaaaagaa atcgaagaaa tttttgactg gacaaatacg aatccttatc tgcaaaaaaa
    10201 agctgagcgt atcaatgaaa tctacaaaca cggtacaccg ctagaaaaga aaattgccag
    10261 cgtcttttta gaaacattct tgttctattc aggtttttat actcctcttt attatttagg
    10321 aaacaacaaa ttggctaacg tagcagagat catcaaacta atcatccgag atgaatctgt
    10381 acatggaacc tacatcggtt acaaattcca attaggattc aacgagttac ctgaagcaga
    10441 acaagaaacg ttaaaagact ggatgtacaa tcttctttat gagctttacg aaaacgaaga
    10501 acgttataca gaagaattat acgatccaat cggctggaca gaagaagtca agacattcct
    10561 acgctataat gccaacaaag cattgatgaa cctagggatg gatccactct ttccagatac
    10621 tgccaatgat gtcaacccaa tcgtaatgaa cgggatttca acaggcacaa gcaaccatga
    10681 cttcttctca caagtcggaa acggctattt gttaggtcat gtagaagcga tgaaagacga
    10741 cgattatttg attggattgg aataaggatt tattttaatt caaaaatttt tcgaaaaata
    10801 aaaagagttt agcgtacaga tattttaaat gtacactgaa ctctttttta tgcttattca
    10861 cttgtttctt ccagcacttt tggactgtct aacttttccg aaacatgttc cattcttctc
    10921 aataaataga ttttccggat atttaatatt atcgtcttaa tcactgcata gacaggtatg
    10981 caaatcagca tgcctaatat tcctgcgaca cttcctgctc caatcaatac caagatgatc
    11041 gtcaaaggat ggatattcat tgatttccca aataacaacg gcttgatcaa atttccatct
    11101 aattgttgga tcactaaaat cgacaacgcc atatacaaag cttgaaatgg agagataaac
    11161 aaaccgacaa tgattgctgg taccgctcca ataaaagggc cgacatacgg gataatattc
    11221 gtgattccgc aaatgaccgc aagcaacaag ctataaggct gatcgaatat tctcattgcc
    11281 aaaaagctca ttacaccaat aatcaaagca tctaagatcg tactgctgat ataggcagat
    11341 aatgtttcgt ttaattcttt taccgtttgg cgtaactctt tacgaatcgc tttcggaaag
    11401 aagtgagaca acgcatcaat aaatttgtgc ccatccttga acataaatag caaaataaat
    11461 ggaacggtaa ataacaaaat aaaaaattgc atcataaatg aaatgatttt tgagacactt
    11521 gcagataaac tgactaatat gatattgaac aaattactaa gagataaatt tgctgatgct
    11581 aattgttctt ccaaattgaa atttttaaac tcttcccgct gcgccaattc atttaaccat
    11641 ttgcttgttt catctgcata cattggcaag ctctgggtca attgaattgt ctgttccatt
    11701 aactgaggca ctacattcat gacagttaaa acaactaaaa tgattacgac caaaaacgtc
    11761 aataaaaaac cgaacactct cgggatccga tgcttttcga gaaaaacaac gattggatcg
    11821 aacatataat agataaaacc agcaacaagc aatggtaaaa aaactgccga aagcattttc
    11881 aatattggac taaaaatatt cggcatttgt aaaataaagt acacgccgat gatcgtgagt
    11941 actagctcca ctgtccagaa aaataatttt gattgtttaa aacgtgattg catacatctc
    12001 ctccaaggtc ctttgatact tctattctat catttaacaa agagttacgc gctgagattt
    12061 tcatttgctt aaaaaaaaca aaaaaaataa acatatttta atgagtagac gagtacttaa
    12121 acaaaaacac ggtttggagt agtgacaaaa attatactgc agtccctttt tccaaataaa
    12181 cggtgttcaa aagcttgcat cttcgtaata agcccagcca aacgaaaaat ataaggaact
    12241 atttttattt ggctgggctt attacttgat gtaggacatc tattttacaa cctcagatca
    12301 tttattgcgc aatactgact aaatgcaact ttgtgatatt tttgtgtacg gacaactcct
    12361 cgatcacatc ggcataagtg agcccttttg gaagatcaat cgtatagata tttttgtaca
    12421 ctcggtagtc atcaacgaat tttacgtcaa aattcacatc ttcgattacg atatttttct
    12481 cttcaaaata atcggtaaga aaatctttgg tttcttttct atggataaat tggatttcta
    12541 actttttagt cgtaggtaca tggatcacgc gtttcactac tgtgagtgca atagcaatcc
    12601 cgataaaact gatgatcgca atggaatagt aacccatgcc gatggcgatc cctaatccag
    12661 caattgccca taaagaagcg gcagtcgtaa gtcccgtcac cgattgtttc gtgacgatga
    12721 tcgttcccgc gcctaagaaa ccgacaccac tcaccacttg agcaatcagc cttgcttcat
    12781 ctgaacgaat cgttccagca agctgtggat actctaaagc atctcttaat gcaccactag
    12841 cgatctcaac ttggatcaat gcaatgatac atgctcccat acaaactaaa atatgcgtac
    12901 gcatacctgc tggacgattt ttatattgtc gttcaaaacc gattgctccg ccaaatagca
    12961 tagcgatccc taaacgtaaa ataatctctg gaattgtcaa taccatatca ttcatctaaa
    13021 caccctccag tccaatatat catgtctgaa aggagcagac aaaaaaagtg caatttttac
    13081 ctcttacgaa aaaaggactg tgacaaaatt tttgtcatag tcctttttat gaataaacgg
    13141 tgttcaatca gctgaccctc ggtattactc aacaaacggc aaaatatggg gagctatttt
    13201 tcctttgtct tctctaatac tcaggtcaaa acgctgattt cacaacctct ttagcctact
    13261 attgaaaatt acaacttact tgctgcttct tcatcgatga tcacaacaac attgtcgtga
    13321 ttttgcaata cacttgctgg caaatgattc gttacaggtc cttcaatcat ccctttgatt
    13381 gcatcagctt tgctttcgcc aaatgccatc aagacgattt ctttactttt cataattgaa
    13441 ccgattccca ttgaaacagc ttttgttggt acatcttctg ctttatcgaa gaatcgtttg
    13501 tttgcattga ttgttgattc tgttaagtca actactgaag tcgttccgtc aaatgaagca
    13561 ccaggttcat tgaaaccgat atgtccattt tgcccgattc ctaagatttg gatatcgatt
    13621 ggatgtgcat cgatgatttg atcatacttt ttgcatgctg cttccagatc ttctgctttt
    13681 ccatctggta caaacgtttc tttgaatggt tttttatcaa ataattggac atccatgaaa
    13741 tgacggtagc tttgttcatc tgaaccgcct agtccaacgt actcatctaa gtttacagaa
    13801 gtcatttcac tgaaatccaa atcgcttgaa gtcatttctt tatataaagt aactggtgtg
    13861 cttcctgttg ccaatcctaa tacttttgca cctttgttca taccttcttt gatgatttca
    13921 aatgcttttt ttccgccttc ttcagcgttt tttacacgaa tgatttccat agtgattgct
    13981 cctttatatt ttggtatagc ccaattatag cattcttttt ttctttttac aataattagt
    14041 atagaccaaa ttgcattttt ttcattttct tgacaataaa taggaatccg tgttaggttt
    14101 cataagatgg ttcaatcaaa caatccatgt tgcatgaaaa ccaagcaagt gattcactgt
    14161 ggacgcttta tgtctgcagt ttttttatga aaaaggagtt attatgagta tactgacatt
    14221 aaacaacatt acccagcaat ttggcgacaa aatcctctat gaaggtgtcg atctgcaatt
    14281 aaatgctggc gaacatcttg ggctgattgg acaaaacgga gccggtaaaa gtacattgat
    14341 taaaatcatc actggtgaga tcttaccaga tgacggacag atccattggc aaaaaaatat
    14401 tcacaaaggt tacctcgatc aatatgtgga agtcgatgaa acattaacaa tcgaagaatt
    14461 tctaaaaact gcgtacgcaa aagaatacga aaaggaagaa aaaattgctt ttctttacca
    14521 aacatatagc gagaccttcg atgattcctt gctggaaaaa gctgggaaac tccaaacgga
    14581 attagaccaa ggagtgtttt atcaaatcga caccttagtt gctgagatga gcagcgggtt
    14641 ggggatcgat gtcttaggtt tagattcact gttgaaaaat ctaagtggtg gacaacgttc
    14701 aaaggtgatt ttagcaaaac tacttttaga aaacccagat gttctgatct tagatgaacc
    14761 aaccaatcac ttagatgatc agcatatcca atggctgacg cagtttcttc aggattttga
    14821 cggtgcgtat atcgtgattt cccatgacca agaatttctt gatcagatca ccacccatat
    14881 cgcagacatc gagtttggaa agattactaa gtatactggc catctaaaac aagcattgaa
    14941 acaaaaggaa caaaatcgcg aaagctattt gcgccaatat catgctcaac aaaaacaaat
    15001 cgaaaagacc gaagcttata ttcgaaaata caaagccggt tcccgatcaa cgatggcaaa
    15061 aagccgacaa aaacaattag ataaaatcga acggctgacg cctccagctt cagcaagtaa
    15121 agctgtcttt gattttccat tctcgcctat tgtcacgaca ttagcagtgg aaacaactga
    15181 tttagtcatc ggatatgatc gaccgctact tgcacccatc aatttgacca tccgctttgg
    15241 tgaaaatatt gccattcgag gcttcaacgg gattgggaaa tctactttat taaaaacact
    15301 gatcggtgag attgaaaaaa taagtggcga tttccacttc ccagataaca caaaaatcaa
    15361 ttacttttca caagatctgt actgggaaaa tccattagaa acaccactac aatatttaag
    15421 cagtcaattt ccaaaagcga ctatcaaaga attacgtcgc caactagcta aagcagggtt
    15481 agttaatcaa ttagcaagtg aacctctcac tacattaagt ggtggggaac aaactaaggt
    15541 gaaacttgca cagttgacaa tgaaccaagg taatttactg attctcgatg aaccgacgaa
    15601 ccatatcgat caagaaacga aagaaagctt acaagacggg atacagaaat ttccaggaac
    15661 agtaatcatc gtctctcatg aacaagaatt ttatcaagat cttgtgcaac gagtcattga
    15721 gattgaacca aaataaacgt ttgattgaat agttgaaaaa aaggagctgt gacaaaagtt
    15781 ttgtcacaac tccttcttct gaataaatgg tggccaatta gctgaccttc ggtattagct
    15841 caaaaaacgg caaaatatga ggagctattt ttatttggct attcttatta cttgatgcag
    15901 aacagctttt cacaacctct tttcgtctat tttttatttt cgagatttct ctttccttta
    15961 aaaacaactg tttgaaagaa gaacgcacat ccttgaaaaa caagcccgct aaccgttaca
    16021 cctaaccagc catactgctg ccacattgtc gtccctagca atgaaccagc cgcacctcca
    16081 ataaagtaca taaacataaa tacagtatta tttcggttgc ttgctgcttc acctagccct
    16141 tgtactcgtg tttgattggc gacttgacca aattgattcc cgacgtctaa taaaataata
    16201 gctaaaagca aaatggccaa atggcttccg ccaaccaata aagcaataaa gcttattgtt
    16261 tgcataacaa gtcccatata cacgattttt ctttcagaat aagaatctgc cagtcgtccc
    16321 acgataggtg cagcaactgc tccagacaaa ccaaaaatcg ctaatatccc tgcttcgaat
    16381 gttccccaat tgtaggcggg actgctgata taaaaaatca acgtagacca aaagatcgaa
    16441 aatgttccaa acatgaaaaa tccactgact gccgcttctc ttaatagacg ttgagaagca
    16501 actaaatgcg gcaaactttt aagtgaatca aggtatgaaa cagctgtact actttgctgg
    16561 acagaacgcg gcattttcca gtaaagcaga agtgtaagaa ttccgactgc acataaagca
    16621 aataaataga tcatccgcca agaaaaaaga ctggcaacaa taccagatac cgtccgagaa
    16681 agcaaaatcc ccacgagtaa accgctaagc aatgtaccca tgaccttccc tcttgctttt
    16741 ggtcccgcta gtaccgcacc gtaaggaatg atgatttgcg gcacgatcga aagtaatcct
    16801 attaaaaaag aagcgcatgc aaataacgta aacgaaggcg caaaaaaagc ggccaacaga
    16861 gagacggcag ataacgctgc cattcgaatg atcaattttg gacgatccac cacgtcccct
    16921 aaaggaacta aaaacagtaa ccctaacgca tagcctaatt gggtcaacat cgtcaatatc
    16981 ccgactgcac ttgtgctaac agataaacta gctgctactt ttgttccaat cggctggatg
    17041 taatacatat tggcaacaac aacgccacaa gtgattgata atagtaaaat aagcgaagtt
    17101 gttaaaggtt ttttttcatt actcttcatt gatttccccc atttcctatc ttagtttaca
    17161 acttatggaa aagattggaa atagaaaaat acaactattc gccacactta ttatttgtgc
    17221 ttttgttttc cttgtttttt cttgcgatct ttataaatga agacaactaa ccctactagc
    17281 acagtaacac ttgcaaccaa cgtgactgca gttttgttat atccgtgaaa cagagcttct
    17341 ccaccacacg catacaacac agacgaaggg atgactccaa tcacgatcga caatatgatt
    17401 tgctgtagag gcaatttcat cgcatctgca gcaaaattga ccatagaaga tggaataacc
    17461 ggtatcatat agcctaacat tacacctatt tttggatgtt tcatttgtcg gattgcttgc
    17521 acccaatgat tcgtttcggt tttcttatct aaaaatttta agcgatgcat aaggaaaatt
    17581 gcagtgatgt taccaagagc attacccaaa atgttgatga tcgcacctgt cagtggtcca
    17641 aagctcaaac cagcaatgac tccaactact gaagtaggaa tacctggaac agcacacatc
    17701 accgctgtca atacgatcaa gagtatcgat gcaaaagtcc catgcgaacg aatatgtgcg
    17761 agtagctctg ccttactcgc tctcggattt aaaaataatt ggatgtctgt ctgatattct
    17821 aaatacaacc gatagcccac taccagtatc agaatgattc ccaacgagat caatatcaat
    17881 cggattattt tctttttcat atttcctcct gaaaaaaatc tatattcatt aaataatagt
    17941 ttaacataaa aacaaaaaag caccgaaatc caacatagga tctcagtgct tttaaatagt
    18001 aaaaattatt taacagttac agatgcgcca acttcttcaa gtttagcttt caattcttca
    18061 gcttcttctt tagatacgcc ttctttaact ggtgcaggag cgccatcaac tacagcttta
    18121 gcttctttca agcctaaacc agttgcttca cgaactgctt tgataacttt aactttttgg
    18181 tctccagctg cagttaattc tacagtgaat tctgtttgtt cttcagcagc agcagcgcca
    18241 ccagcagcag ctacagctac aggagcagca gcagatacgc caaattcttc ttcaatagct
    18301 ttaactaggt cgttaagttc taggatagtt gcttctttta actcagcaac gatgttttca
    18361 atgttcaatg ccattataat ttcctccgtt taattgttct tttttattta atgctttatg
    18421 cggcaattaa gctgcatctc catcttcttt gtctgccact gctttgacag cgtaagccac
    18481 gttgcggact ggcgcttgta gtacagatag caacatagat agaagtcctt cgcgatttgg
    18541 caattttgcc aatgctgtga tttcttccaa tgaagagact ttaccttcga tgataccgcc
    18601 tttgatttct aatgcttttg cttctttagc aaattcgtcc atgattttcg ctggtgcgac
    18661 tacatcttcg ttactgaatg caacagctgt aggtccagtg aatacttcgt ccatgccttc
    18721 aaggccagct tttttagctg cacgtgaaag aatagaattt ttgataactt tcatttctac
    18781 gttagcatca cgaagttgtt tacgtaaacg tgttacttca tcaactgtta atccgcggta
    18841 gtcaacgact actactgatg cagctgctgt aaatttttcg cttacttcat caacgattgc
    18901 tgcttttttt gcgataattg cttcactcat ttttgagttt cacctcctga attttgatgg
    18961 aagagttttt tgcactgaaa aggcaagtca cttttatagt tactttgctc aaccagtatt
    19021 attcatcacc tattcacttc gttcatagtg attctactcg ctttgctcga aaggaaactc
    19081 ctttcgcttt gctcaaccag tattgttcat catctcttca cttcgttcat agtgattcac
    19141 aataaaaaac tctatgccac cgcagacata gagggactat tgaatatatt caataaacgt
    19201 cctcggtagg aaattaaggc ttgatgccac ctactgtctt cggtacaagt taattattaa
    19261 cctcaactag cttagcatgc taaactagtt gagtcaagaa ttatttttca gttggatcaa
    19321 tcaacattct aatatttaat tagaaagaag cttggtcaac atggatacca ggtccaaatg
    19381 ttgttgtgat cgttacattt ttcatgtatt gacctttagc tgctgatggt ttagctttta
    19441 gcaatacatc atggattgct gcaaagtttt caactaattt ttcatcgtca aatgaaactt
    19501 ttccgattgg tacatggata tttccagctt tgtccacacg gtaagttact ttaccagctt
    19561 tcacttcgtt tactgctttt gttacgtcca ttgtaactgt accagtttta gggtttggca
    19621 ttaagccttt tggtcctaat actcgtccta gacgacctac agtagccatc atatcaggtg
    19681 ttgcaacaac tacatcaaag tcaaaccatc cgttttggat tttttgaacc atgtcgtcgt
    19741 cacctacgaa atctgcacca gctgcttcag cttcttttgc tttgtctcct ttagcaaaaa
    19801 ctaaaacagt ttgtgtttta ccagttccgt ttggtaatac aacagcacca cggatttgtt
    19861 ggtctgcttt tttagggtct acgttcaaac ggtatgcaac ttcaactgtt gcatcaaatt
    19921 ttgcaatgtt tgtttctttt gctaaagcta ctgcttcggc aacaggataa actttacttg
    19981 aatcaacttt ttttaatgca tcttgcattt ttttgctctt tttagccatt ttggttcctc
    20041 cttgattgtg gttataacgg ttgtacctcc cacgtgttct atatctttca atatagagcc
    20101 aactgagaag ctgctgtctt agggtgtata gattattcta cagtgatccc catgcttcgt
    20161 gcagttcctt caaccatgcg cattgctgct tcaacgttag ctgcgtttag gtcttccatt
    20221 tttaattcag cgatttctct tacttgatcg ctagaaactt tcgcaacttt atttttgttt
    20281 ggttcgcctg aacctttttc gattttagct gcttttttca atagaacagc agctggcggt
    20341 gtttttgtaa cgaatgtgaa tgaacggtct tcgtatacag aaataacaac cggaatgatc
    20401 aaacctgctt gatcagctgt acgagcgttg aattcttttg tgaatcccat gatattgata
    20461 cccgcttgac ctagtgcagg acctactggt ggagctggag ttgctttacc tgcaggaatt
    20521 tgcaatttaa cgattttttc tactttcttt gccacgagac atacctcctt aagtccgtga
    20581 tgtggttaat tggagatgca tatttctcct cccacgtttt atctgatctg aaaaaatcca
    20641 gacaaataat gtgcaaaaca atgcacactg gtacagtata gcaaatttat ttgttttttc
    20701 aagatttttt tctgctattt ttacattttg cttgaataaa cgctaaattt attctaaaaa
    20761 aacaacaatt tttcgttctc atttcttctt ttatgttaca cttgcgctag catggttcaa
    20821 cagatgaaag caaacaataa cacggttttt tagacccttg tacaaagatt cctacataac
    20881 aaaatgaggt ggagaaaatg aaagatttaa ggtatagaag tgtctttgat attattggac
    20941 ctgtgatgat tggtccaagt agctctcaca ctgccggagc tgctagaatc gggaaaatca
    21001 tacgtagtat atttggcgaa cagcctgatt ctgtagatat ttatctatat gaatcatttg
    21061 caaaaaccta tcgggggcac ggaacagata ttgcattagt tggcggcctg ttagggatgg
    21121 aacccgatga tgaacgttta gctgattctt tgaaattggc atatgaagcc ggtatggaag
    21181 tcttgtttgt tcctaagagt gaaaaagccg aacatccaaa ctctgtaaag atgcttctaa
    21241 aaaaaggaac acgaaagctt tcagtcactg gcatttcgat cggcggtggc aatatccaga
    21301 tttctgaatt gaatggattc aaaatttctt taagcatggg tactcctacc ttgatcgttg
    21361 tccaccaaga tgttccaggt atgattgcta aagtgactag tatactttct gagaccaata
    21421 cgaatatcgg cactatgaca gtgacacggg aatcaaaagg tgaaaatgcc attatgataa
    21481 ttgaagtaga tgatcctcaa gtagaagaaa cggtaaaaag actcaaacag ctgccaaata
    21541 tcgacagcgt aaattatttc gattaaggag ctaaaaaaat gtttttatcg attgaagaat
    21601 tagtagatca agcaaaagct tatccaagtg tagcagaatt gatgattgca gttgaaatgg
    21661 aaatgagtgg ccgttcccgt agtcacatca tcgagatcat ggaaaaaaac ctctcagtaa
    21721 tggaacaatc aatcaacgag ggaaaagaag gtgttacctc cgtcacagga attaccggtg
    21781 gtgatgcaaa aaaattagat gattatttaa atgaggcaga ctttttaagt ggagaaacaa
    21841 tcttaaaagc tgtacgcaat gctgtagctg ttaatgaagt caatgcaaaa atggggttga
    21901 tttgtgctac accgactgcc ggaagcgcgg gtgttgttcc tggtgtgctg atggctgtcc
    21961 gagatcggct tgatttgaca catgaacagc aattggaatt cttatttacc gctggcgctt
    22021 tcgggttagt catcgcaaac aacgcttcta tcagtggtgc agaaggggga tgtcaagcag
    22081 aagttggttc tgcaagtgca atggcagcag cagctttggt ttgtgcaaaa aatgggactc
    22141 ctcatcaagc agcacaagca gtagccatta cattgaaaaa tatgatggga ctgatttgcg
    22201 atcccgttgc aggcttagtt gaagtcccgt gtgtcaaacg taatgcttta ggctcttctc
    22261 aagcttttat ctctgctgat atggcattag cgggtatcga aagtgtcatt ccgccggatg
    22321 aagtcgttgc tgcaatgtac caagtcggac gtcaaatgcc gtcgattttc aaagaaacag
    22381 cagaaggtgg cttagctgtt acgccaactg ctaaacgact tgcggaagag attttaaata
    22441 agagggcgtg ataaagctcc ttaatttcga gtattaagag attagttatc ttcataacaa
    22501 aaaacgaaga gaggactaat tgcccttctc ttcgtttttt attaatccat caatttcgta
    22561 ttttttactt catcgatcgt ttttgttcct gcaagttgca tcgtgatcga caattctttg
    22621 ttcaggtgtt caaaaacaga tgttactcct tctgccccac ctagattcaa cccataaatc
    22681 actggtcggc caatcgcaac taagtcagca ccacttgcca gagctttgaa gacatgctct
    22741 cctcgacgga caccagagtc gaaaatcact ggtacacgtt tgttgactac ttcggcaatt
    22801 tttggcaata cttcaaagga cgcaggacct ccatctagtt gacgtccacc atgattggat
    22861 acccagatac catctgcccc tgcagagata gcgattactg catcttctgg cgattgtatt
    22921 ccttttacga taactggaag atgggtgatt tctttgattg ttttgatgtc atctggtgtc
    22981 aagccttgtt ttgcagctgc ataaatttct gcgattcctt ttccttcgcc attaccactt
    23041 gcactttgtt cgctatacgc agctaagtta ggcattggta atggaaattg gaattgattg
    23101 atgacgtctt cttcacggta accaccgagc gttgaatctg ccgttaaaat gatagcttta
    23161 gcccctgctt ctactgcttt gttcaaaatg aactcattga atctgtcgtc tttactcata
    23221 tataattgga aaaactgcgg tgcgcctggc gctgcatcgg ctgcgtcttt gattgtcgta
    23281 tttgcataag tgctgatgga aaagattgat ccagcagcag ctactccttt agctgtatca
    23341 gcttcccctt tttcatgtgc caatccttgg gccgctgatg gcgcttggat aatcggtgta
    23401 tctaattcaa ttccgaaaac agatgtatgc aaatcggctg agtcgattcc gcgtaatatc
    23461 cgtggcatga ttttctttgt attaaaagaa gtcgtatttt ctttcatcgt ccactcatct
    23521 tcagaaccac cgcgaatata gccaaaagct cctgcttcca tgcgctcttt tacacgacct
    23581 tctagttagg caatgtttac aatatcgatt ggatgttctt cattacttgt atgataagtc
    23641 atagtagatc cttctttcat cttcttgatt acatataaat catactaaac ttacaaaaag
    23701 taagcaaaca tttactctca gatactttta tcgctattct ttccaaataa atcgacttac
    23761 aagctgatct acttcttggt tttcatgaag catactatga gaggcatttt cacctgtcac
    23821 tactttttcc tctatttcat ttccgcgttg cttcaacaac gcatagaccg ctaatgcact
    23881 gtttaacggt actgttccat cactcaagtc tgtttcatct agttgaccag caatcaagaa
    23941 aaatcgagtg gttataggaa tattaccgat catttgctgg taatcttgat agcggttaga
    24001 aacgacagca ggcccctttg ctaactcatc tgtcagagac tgcgcactat catcgacaaa
    24061 atcattaaaa ggcgcgccaa ttgctgttaa tgtctgaact tgtggcaatg acgtatcctg
    24121 tccatacgtt cctaaatagc gtagtgcact aactcctccc atggaatgcc ccactatgtt
    24181 gactttatct atgccgtagt tttctttcag atacaaaaga caagttttga tccattctgc
    24241 ttgattccac tcgttatttt tattatcttc aaacaatact tggaccatcg gattgtctgc
    24301 ttttccagac aaatctcctg ttgcttgtat ttgtccatct gttcctactg ttaaaaccaa
    24361 ttcttttact gcttgctgat ttttttccaa acgccgcaac atccctttaa aggaattttc
    24421 tgtcccgctg tagccgtgga aaaaaagtgt tggaatagtg gttttttcta tcactttact
    24481 ggattctgcc gttttttctt tatcagaaac ttcagtagta tttatcttcg tcgtctggca
    24541 accacttaac gcaaacaaac tgataaggac taaagctgct gcagttgcac gtttgcataa
    24601 atttttgatt ggttctgtca tacacctctt cctttccata tagaaaaaag aaagacttga
    24661 aaggccaatc atatgatcga catttcaagt ctctttatta atcgatttta tctacttgat
    24721 cgaaatcaag ttctgtactg gtttcacgtc caaacatatc gatgttgact ttcaattttt
    24781 ctttttctcc gtcaacttct gttacttgtc cttctaatcc agcaaatgcg ccttcgatga
    24841 ttttcacgac ttcgccaact tctacttcta gatcattttg tcgcaggctc ataccgatag
    24901 aacgcaagat atgattgact tcttctggaa gtaatggtgc tggtttgctt ccggctccgt
    24961 gtgaaccaac gaatcctgtc acaccaggtg tgttgcggac aacataccat gaagcgtctg
    25021 tcatgatcat ttcaacaagt acgtatcctg ggaatgtttt atgaacgatt tctttttctt
    25081 tcccattttt gacttcagtt tctttttctt caggaacaac gacacggaaa ataaagtcgg
    25141 ccattttcat actttgcgca cgagattcga tatttgcttt tactttgttt tcatatcctg
    25201 aatatgtatg caagacatac cagttttttt caaaagattc cattgaaggc tcctcatttc
    25261 attgtaaaat aaaaaaacct cagtacaccc gaagtttttc tttcgcttca ttatagcact
    25321 aaaagtacgt tgtggctagc gagttatttt aaaatccagc caaataagct ttggatggct
    25381 gtatccatta cgtaaaacat caatccgaaa ataatagaag tttcgatgac tactaacgta
    25441 tctttacgga gttgttttct gcttggccaa gttacttgtt tgatttcatc ttttacgctt
    25501 tttagaaatt tcatgagaca acctcccttg ctactgttta tttcgtttct ttatgtaatg
    25561 tatattgatt acaatattta caaaacttat tgatttccaa acgttcgccg ctttttcctt
    25621 cactgactga ttttgtgtag ttgcgagacc cacaaacgga acaagcgagt gctgcttttt
    25681 tctttgccat ttacttctgc ccccttgctg agaatcagac ttatacttca taacagtatc
    25741 acgtaacttt ttacttgtca atttgtgaaa taaactatct attgttttgg ataaaattat
    25801 gatatgatta atttgaaatt aagctaaaat gagacaattg gaggtatagc gatgctatta
    25861 aaaacacttg tatataagaa aaaagatctg acaaccgtta cagagactag tacattagaa
    25921 gaagcattaa aaattttaga aacttccgga taccgttgtg tgccgatctt agattcttca
    25981 ggtaatattt tccgtggaaa catttacaaa atgcatatct atcgtcataa agcaaatggc
    26041 ggtgatatga cgcttcctgt cactcatctt ttgaaaaacg ctacaaagtt catttacctg
    26101 gatacttctt tcttcaaagt attctttacg atcaaagagc ttccttatat tgctgtcctt
    26161 gacagtgaaa atcattttta tggtattttg acacatagta cgttgcttaa tatgctctct
    26221 caatcttgga gcgtagaaca aggaagctat gtactgacaa ttgcttccac aggaaagcaa
    26281 ggagatttgg ctgctattgc aaaaatcatt tcaaaacaca gcagcattgc cagctgtatt
    26341 actcttgata tcgaaaaaga cgaatacatt cgacgtacat tgatcacgct accggctaac
    26401 actgaagcga aagtatgtga aacgatcgtc aaccacttag agaaaaagaa ttataaagtg
    26461 gtagaaattg aaaatttgca aaaccaagaa gcataatcaa aaagcgaaaa aagaaaacag
    26521 ctaatctgct ttcttttttc gctttttata attagacacg cttttcaaaa tcaagcgcgc
    26581 gatctaaata atacatcaat ggtgctgaaa cagccatgac gataaactca cttaatgcaa
    26641 gcgtgccata ggttgtccaa aatgcttggc cacctgttgg aactaacata taagcaatca
    26701 gtcccatgct gatggtaaag aagatcgtgt tcaatactaa acgcatcttt acatttttca
    26761 ctttgttttg caataaagct gtcaggccta aagaaagcaa tgtttggccg ccaccaaaaa
    26821 ccacatccat gataccaaaa ccaaagaagg cattatagac gatgaccccg cctaaaactc
    26881 cccaaagcaa tttacggtta aagacaacta aatgattcaa actttctgaa atacgaaact
    26941 ggatcggccc tgaggataca ggcgcaacta aaatagttaa cgctagataa agagccatga
    27001 tgatgccatt tacaacgatt acggataacc gttgtctgct tttcgttttt tgcattttct
    27061 ctcctctttt ctcctagttt ttttaacgcg ggatggttga tgaaccgcgg ttatgtgaaa
    27121 caaacaataa ttataacaga cttttcattt atttcaatcc gtcttatctg cttttaaaaa
    27181 agcaaaagct taaacaatgt aaacgtttgc taaaaatcgt gggatatggt ataaagtaag
    27241 tgtaaggggt cataagaatt cccagtaaag cagacgaagt aggatctgca agaacagaat
    27301 tgttgtgaaa cttaaataaa taaaaaagga tgtgggaatt tgagcaacct cttactagag
    27361 aacggtctag caagcagctg aagatctaag tggacatgcc gaaccttgga acttctcctt
    27421 acgtaagtaa tggatcatgc gcttgctagg cttttttgtg tttttcggtc ttcggacctt
    27481 tttcattttc ctagacttct gctataataa ccaagaatta attttgtaac agattttttt
    27541 gaaaaaacaa gtttctttca ttcaaaaaat agaaaaagct tgctaattat tggttaacca
    27601 gcaaacagga gtattaaaat atcttgtttt attgaatagc aggaggaaat catgaaattc
    27661 cattatcata tattgatcaa tccatcggca ggcagcggaa atggctataa agtagcagaa
    27721 agaattttac cggtattaaa aaataagcat attgactata cacttcatta tagtgaatac
    27781 aaaggacacg atgctgagat tgctgaaact ttggcaaaag aaacattgat tccttggata
    27841 gaagaagaac agaaaacaat cgatactttc ccccttttga tcatcgtagg aggtgacggc
    27901 accttacatc aggtacttga tactttctat cagatggaag tagaattccc agttgcttat
    27961 attccagcag gttctggaga tgattttgct cgaggggcag gccttccaaa aaatcccaaa
    28021 aaaggacttc agctgatttt agcagctcaa tcaccagaaa aagtccacat aatggcttat
    28081 gaagaaaaaa taagtgaaaa aaaaggaata gctgttaata atttcggtat aggattagat
    28141 gcagctatcg tccatgcgac aaatcattct tccacaaaaa agcgattgaa caaatacaac
    28201 ttaggatcat tttcgtattt attctctatt ctccgtgctt tattcactca aaaaggattt
    28261 ccaattctgg tagatgcagg aggaaaacaa ttaagttttt caaacgcatt tttatgtaca
    28321 gcaacgaagc atcctttttt tggcggagga atcgcgattg ttccaactgc tgatgcgtct
    28381 aaaccagtca ttgattttgt cgtggtcgaa cgaatcaatt tctttaaaat cttgtggctc
    28441 attcttttac ttatacagaa aaaacaattg aaatcgaaat atttccatca ctacacaaca
    28501 aacagattac gtatcgtttc aacaacaccg caacatggtc aagaagatgg agaagaaatg
    28561 gaaaaacgac cttttgatat cacaataacc acaactaaac agttgttttg gtgttaataa
    28621 aagaggtttt gtcagaagct acatctggaa caccgtttat tcggaaaaaa ggactgtgac
    28681 acgacttttg tcacagtcct ttttttacta tgcagttaat tctttgccca attgggttgc
    28741 catcgatagt ttctttcttt acccaaaata aattggatca ttgaaaacac cgcagcaaaa
    28801 gcaactaaga cacaaccatt gaaccaaaaa gattctgcaa atgtttgatt gtaactgagt
    28861 gtttcactga tcaagttatt attttgaaca aaccaatatg ttggtgtgaa tgccgctatt
    28921 ttgctaacca tttctggcaa gtattctgct ggtacaaaca ccccaccgat aaagcaactg
    28981 cctgtgataa aaatatcttt gatcccaccg ctggtttctg agttttttac caatgaagtg
    29041 atacaagccg aaaagctaat gatcggcaat aaaaacaaga aagtatttaa aatatgatac
    29101 cataccgtta tatcccacga ggtgtggatc actaacagaa ccattagaag taagaagcca
    29161 ctgaacaaca gcagcgagta actaatcaaa ctaaaggtta ctttgcgatt caagtttcgt
    29221 ttggtaatgg gcgcactctg gtttcgttta ctgatctctt tgcgattaaa tgctagattg
    29281 atgacgctga aaccgctaaa aatggataga aacaacccat aagacaagaa attaaaggtt
    29341 gtgcccgtca acagcttttt catccgctga ctatatcccg cacttagctc aacttctcca
    29401 gtaaccgaga gtgccatttg tgtctgttcc atagctgcat cgattgtttt atcttcttct
    29461 tgaaacagaa tcagtgtttg caaaaaggtg ttcacttgct gtgtaacaac tgttttagaa
    29521 aaggcatctg ggcgactctg tatcttgatc gtcggcatct ttcctacttc gacttgttcg
    29581 gtaaagtctg caggcagatg taagataatg tttacacgat taaaataaag agcatcatcg
    29641 atttcttttt gtgaggttcc atccaatgta acgactgttt ccttgtcatt caaatattta
    29701 cgaaggccat cgactacagg cgactcttct tcactaatca aggcgatttt cgcatcttgc
    29761 agagaggtat tttcaacatc aatattttgc gcaaaaaaga atgtggcaac aacagtgata
    29821 attacaccta tcagtaaatt cgatttattt gctttaagca atgtccaaat cgttttataa
    29881 agcgtcataa cgcacccctc tttccatcca agcatttcct aacgcaaaaa ggaaaataaa
    29941 tccgatcaac catagaagat tggtataaaa actagccaaa tcagtgtaga aataaagttt
    30001 atacaaactt tcgctgatca agttgacgag atttaattga ccaacaatcg gaatatgaag
    30061 atcaatccaa tacttcactt gttccgaccc catcatacca gcaaaaaagc tcatcgcaat
    30121 cgtcactgaa atcaagatgc tatccttttg caccagattc atttttggta tcaaattacc
    30181 aaccaatgta cctaacaaaa tcgcacacaa cgaccccaga gcggtaacaa tcatcaacca
    30241 ttgccaacgt tcaccaaagt ctacttgata gataaaacga aagacagcta acataatata
    30301 aatctctgtc aaaacaatcg taaagcttgc tgccaaatta gcgattgaaa ctaacgattg
    30361 ctttctagga atcaaggaca agcgaatacc atttgacgat tgatttgctt gctgatcctg
    30421 tacattgcgg accccccaca tcgtcccgta gagaatcgcc attccaatca gagtgaagaa
    30481 ataaaaactt ttgaaactca tattacgacc tgcatgaact tcttgaaaag atacttcttg
    30541 ttcaaaaaca gtcatgatgg gcgcgagatc aacgttctcc acttctgcca ctttactcga
    30601 aattatacca atacgctgta aaaattgatc catgacgttt ttcatcaacg tctgactcat
    30661 tcctgcttga cccacatgga gcgaaacaga tgaaccatca atgagataaa aaccagccaa
    30721 atcatctgca tttagctttt cattcgcttc tacttccgat agttcctgta cttcaaataa
    30781 tggtttatca ttgacttcga cttgttctaa gactgtggta aaattttccg aaagcgctgt
    30841 ttgatccgct ttgattacaa ttccagtatt aatcgtttcg agattgccac cttgatcaat
    30901 ctcgccaaag gccgccataa acatcaatcc taaaagaatt ggaaaggcca atgtccaaaa
    30961 caacatggat ttatttcgca gcagtatttt tgtgcgatat ttataaaaat gccaaaacat
    31021 tatttcccct cctctagtct ctcaaatcct tgccagtaat ctctaaaaag acatcattca
    31081 aggacgcttc tttggttgct atttttccgt aactgatctt ttcttcttgt aagattgtaa
    31141 gaatcggcaa caatactgta tttagttcag tagttgttac ttggagctga ccttttttat
    31201 aagcgattcc atcagaagct attgttcgct gacaacacgt tttctgggag taatgggatg
    31261 tcgatcgtca aacgatccgc cgtccgtacc atatctttta gagattcttt ggttccttta
    31321 gcaatcacct gtccttgatc catgatcacg atctggtcgc aaagaatttc tacttcttcc
    31381 atgtaatgtg ttgtatagac aatggttgct ccttgtcgat tcagctcctt gatactttct
    31441 aaaattttat tccgactttg ggggtctaca gctactgtcg gctcatctaa aaaaattaac
    31501 cgcggcttgt gggcaatccc acatgcgatg ttcaaccgac gtttcaagcc accactaagt
    31561 tctcctggac gaaacgaaac atatttttct aatcccacta attgaatcac ctcttcaatc
    31621 aaggctttcc ttttctcttt ttcggaaaca tataaaccgc aaaaataatc gatattttct
    31681 tgcacactta gttcgtcaaa gaccgcaacg tcttgaggaa cgaccccaat cattgccttg
    31741 acagcataat tttcaggagt cattgttgtc ccgaacaatt taatggttcc tttgtcataa
    31801 tttaacaaag aaagcataca gttgatggca gtcgatttcc cactgccgtt tggtcctaac
    31861 agacctaaga cttctccttc ttgtacttgc agatcaaaat gattcaatgc tacaaaatct
    31921 ccatagcgtt tgactaattt atcaatttcg ataatcatca tgaaaaactc cttccaaatt
    31981 tcttgttgat ctgactataa taaagtgcaa gaaaagaaac tagtgacgat tggctaaacc
    32041 acacatgaca tttgtcacaa tgaaggagtc tgctatgcat aaatctcggc tactcaacga
    32101 aactagcttt ctactggtta tctcattgct tttgctggta agtcaaggat tcgttgctat
    32161 gcaagttatc tatttattga tcgccgtttt attcactggt gtcagttatt tactgcctac
    32221 aaagccaaat tggattttcc aagttattct atttctttta tgtgcctttt tacctgatct
    32281 tgtcctgttt ctgccagcga cgatgcgagt gatcgcccct gaagaaagcc accttgatta
    32341 cttgtttttg ttaggttttt tgttgatttc gacggttggt aatcatcccc ccatcaaggt
    32401 cgcctttgtc gtaattttgc tgtttttttc cttttactta cgaaaaaaag atcaacgaac
    32461 gaccgatctt gagaaaaagt acattgtctc caaagatact cattgggaaa aacaacaaga
    32521 gttatcggaa caaaataaag cattggccct gtcacaagaa acacaattag ccttgcaaat
    32581 tgtggaagaa cggaaccgaa ttgcccgaga catccatgac aatgtcggtc atctgctgtc
    32641 aagtgcgatt ttacaaattg gtgcgcttga aatgatcaat caagaaccaa aactaaaaga
    32701 accattagaa aatttaagtg ccaccgttca cactgggatg gatcggattc gtcaaagtgt
    32761 ccatgacctc catgagacat cgatctcttt tcaacactcc ttagagtttc tgatcgctga
    32821 ttttccttct tctgttacga ttgaaggaaa cttgtttgaa ggattgaatg aaacgcagca
    32881 aaactgcttc ttaatggtca tcaaagaagc cttaaccaac agtatcaaac atagtcatgc
    32941 caaaaccgtt gttttacatt ttcgaacgtt gccggccttt tatcgcttgc aaatcacgaa
    33001 tgatggcacc agcccacaaa aaaattctgc tcccggtatt ggtttgaaaa cgatgcgtca
    33061 acgtgttcaa gaacaaaagg gacaagtcca tattttccaa gaaaaccagc aatttcagtt
    33121 aacgatcatt ttaccaaggg agggacacca atgataacag tagtcattat tgagatgatc
    33181 catttgtgac gaaatcgtta caaaccattt tagaaagcac gaaagagatc cgtgtgttag
    33241 gcattggtca ttgtgccaag gatgcgctag tcttatacga aacccatcgt cccgatgttt
    33301 tgctgacgga catccgcatg cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg
    33361 ctaaatttcc caaagccatc cttttgcttt tgacgacatt caaagatgaa gaatacatcc
    33421 gcgaagcatt ttccatcggt gtcaaagggt atttaatcaa acagaacttg caagcgatta
    33481 ttccttctgt caaatccgct tataatggac aggccgtatt tggtaatgaa atcatcgaga
    33541 ctttcactca aatgatgaag aataaaccga cgattgatag tcatgcttat gcttctttta
    33601 gtgaacgtga gcttgcaatc atcaaagaag tagctgccgg gaaaaacaat aaagaaattg
    33661 cagatgcctt gtacttgagt gacggcactg ttcgcaatta catcagccaa ttactagaaa
    33721 agctagattt acgggatcgc acgcaattgg ctatctattt ttatcagcat ttgcaataag
    33781 tcttatctaa cacaaaaaaa cctgaatgat gctccttcgc atcattcagg tttttttcac
    33841 atcttctatt caattgtccc gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc
    33901 agggctgcca ggattcgttg gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc
    33961 taacgttttt tctagttttg attcttcacg atcgtctcgt tctacatcag gactttcatt
    34021 caatttgaat tctttttcca tctcatctaa ctttttaaag tcattgccag attcttttgc
    34081 taatttcgct aatttatctt cggtttcttt agccataaag taacgccccc cttttgattt
    34141 cagcttagca tatcacactg aaaaaacaaa agaaaacgct aaccaacgat catttttagc
    34201 tatttctcat ctttaaaatc aagcattttt tgccggtata aaatcgtagg gagaactgtt
    34261 ttccatacca atcatcggat ctactaactg gttatcgatc acttctgctg taagaacgaa
    34321 atctttagaa acagcaggtt ctacctcttc tttcttttct tctccaacaa atgaagaaaa
    34381 cagatctggt tccactgttt ccattacttg gtttttcgtt tcattcgtcc cactgtcatc
    34441 ttctacaggt agcttttctt ttacaacccg tggctctttt gtatagttcc catcttcaaa
    34501 tggatcattt ccgaccaaag aatcttcata agctgctagt ttggtccgga gagtgatatt
    34561 cattttatca ttatccatga tccttgattt caacgtagtc atccggcttg ctgattcatt
    34621 cgctacacaa gtgacatagg cttgctcttc ccctaacaaa atcaacagtt ccttactgcg
    34681 agtcactgcc gtgtagagca aattacgctg aagcatccgc tgatattgat ggaccatggg
    34741 caaaataacc atcttgaatt cactgccttg tgatttatgg attgagcaac aataagaaag
    34801 agtgatttta ttccattcgt ttcttttata agtgacctca tttgcatcaa actggataac
    34861 taattcatcc actttatctt cagaatcttt tgccaagatg atacccgtga tttctcccat
    34921 atctccattg aatacattca attcgggagt attgactaat tggaggactt tgtctcctat
    34981 ccgataaacg gtgtcgttcc aatgtacttc cttttttcca ggtttccctg gattgaatat
    35041 ctcctgcatc attttattta acgcatcgat ccctgcaggt ccccgataca ttggtgctaa
    35101 tacttggata tcttgtgcac ggaatccctt atttttcgct ttttccacaa ttttagagat
    35161 caagggttca atttgataag catcacttcg gaaataggaa cgatcttttt gattctttgt
    35221 gaaatctttt ggcagacgtc cttctttgat ctcatgggca agaggaataa tactagaacc
    35281 atcaccttgc cgataaatct gattgagttc cattttggga atttgttcga tttccaaaag
    35341 atcatggagt acttgaccag gaccgacaga gggaagctga tctttgtctc ctactaagat
    35401 aacttgcata ttcgtcggaa tcgctttgaa taatgtgtta gccagccatg tatcgaccat
    35461 agacatctca tcaacaatca aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt
    35521 tttttcccgt ccattcagcc ccagaagacg atggatcgta ctggcaggca gacctgtcgt
    35581 ttcgttcatt cgtttagctg ctcggccagt tggtgctgca agtagaatag gaaaaatttc
    35641 ttgggtatat tttgaaggat ccaattccag tccgttcaac tctgcaaaaa gattgacgat
    35701 tccgttgatc acagtagttt tccccgtacc tggtcctcct gttaaaataa atagagggga
    35761 acggattgct tcagcgatag cttgttcttg cgaaggtcca tactgtatgc cgaatatttt
    35821 ttcgatacgc cgaatattct tctgcacttc tttttcagga taatggattt cttttttacg
    35881 actgagaagg cgctggatcg aattaccgat cccccattca gaaaagtaca aactgttttc
    35941 gtaaagatta gtctcttctt gttgtatctt tccttcttca acaagttgga taattacagt
    36001 agcaacctga tcaggctgga tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg
    36061 ctgaagcaat gtctccgccg gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat
    36121 ctgatgcaag atagctgctc gaattcttct cggtgaatct gctgcaatac cgatttgttc
    36181 ggctagatta tctgcacgtt tgaacccgat tccttctatg tcttcgacta gctggtacgg
    36241 attttcttcg atgatatcca aagactcgtt cttataggcc tggtagatac tgaaagctaa
    36301 ctgactaccg aacccgtaac gattcagtcc catgatcact tgatccatcc cgtggtttag
    36361 ccgaatcgtc tcagcaagca tttctctttt tggcttcgtc aaaccggaga tttgttttaa
    36421 taccgaagga tcagcaatga ttttttcgat agcatcttct cccagcaatt ctacaatttt
    36481 ttctgctgtt ttcttcccga ttcctgggaa tttttcgctg gaaagatacg ctacgatccc
    36541 atttgcagtt gttggctttt cttgctcata tctttcgagt ttaagctgcc ttccgtattt
    36601 aggatgatcc actaattctc cataaaaacg ataagtctca ttttcttgaa tatcgccgaa
    36661 acttccggtg actacaatct ctttttcacg gtaatcagct gtggtttcac tgatgtgtac
    36721 caaaagcact ttgtaaaaat tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac
    36781 gaaataaggc atttcttctg gctgcatcgg aagccgcctc cttcttctat agtaaaggtt
    36841 ctggtgctgt ttcagttcct aaaaaatctg tttggttcca tactttcagt tcatatggca
    36901 ataatctgca aggctcttca gtctctaaaa tcgtcaaact actattgaac aatccaccca
    36961 tttcacgcaa ctcacttagc tcttttcctg tcatccattg aatggctgca gttaaagaag
    37021 ctccatgtcc tacgaataaa agtgggccat ctttatgttg ggcaaccgca tctgctacaa
    37081 cagtttcgat acgtgtgatt gccgattcaa tcgtttctcc gtgaaaagga gaaggatcat
    37141 acagatctaa atgattacgc aaattcggca gattatccgg atatttctgg tacatttcat
    37201 cgatcgactg accttctaaa gtacccagcc ctagttctct tagcttatct gtataaaaaa
    37261 tctctaccgg acgagcaagc tctttgttga taccttctgc tgtatcacgt gctcgctttg
    37321 ctgtactgga ataaattgct ttaaaaggaa catattttac ctttttccca aatgctttga
    37381 tttcttcgta actttgtggc aaaagcggag aatcgccatt gctcccttga aaacgtcttt
    37441 cctgattcca ttgtgtcttc ccatgtcggg taaaatatag ttccatacgc agcaaactcc
    37501 tgtctttctt ttattttact ctataataaa ctgtctaatc cagcttgtca ttttcatagt
    37561 gatgcgtcaa ttctaaatta gggaatcctt gctggcgaag tgcttcatac actatcattg
    37621 cagctgtatt cgataaattc aatgagcgga catgctcatc attcataggg atgcgtaaac
    37681 acttttcttc gttttcacgc ataaatgttt ctggcaaacc ggttgtttct tttccaaata
    37741 aaaagaagtg ctcttctcca tcagcaaaat tttcgtcact atatacttta tgggcaaatt
    37801 ttgagatcaa atgtagttta cgattaccta aaaatgttaa aaaagcttct aaattactgt
    37861 ggtaagtaat atttacatca ttccaataat ccaaccctgc ccgttttaga tgtttgtcat
    37921 cagttgaaaa acctagtggc tcgattaaat gaagcggagt attcgtggct gcacacgtac
    37981 gggcaatatt tccagtgttt gcagggatta atggttcaaa taaaacgata tgattcatca
    38041 ggatcattcc tctcatattt atgttcgttt ctattttctg acaatttgat ttttgcataa
    38101 aaaaaaacgt atcaactcgg tgtcagtcac tgcgagtcgt tacgttagtg aagctcttct
    38161 cactaacttt tatcagaaca ggaacctgat aaaaaccaat gaaaaaacaa cttgttgcta
    38221 atataacacc ctcaactttt ttttgcaacc tctactcatt aaaaactgct agaaaaacgt
    38281 tttctcttag ttccttttaa taattctatg tcaattgtaa tcttcttcaa aggattctta
    38341 tataagtctg ctttgattcg ctcttctacc tgccattcta aaggtgacat ggccaaaaga
    38401 tctttttgat tttcctctgg tatctcaaat tcataggtta cacgctgacg ttcagttaat
    38461 ttcattgctt cagcaaactt ggctactaca cgttcattgg aataactctg tttttcaggt
    38521 tgattcggat aaaaagcctg acgtagttcc tttaaataat tttcttgtgg caccaccttc
    38581 aacacttctc catcttctga gagtacacgt tggaattctt ggtaatgaga aggcgaaaag
    38641 atatttaaaa ccgcgttgaa tgtttgatca gcaaacggca aattggtcaa atcagctaca
    38701 caccaaaatg cttcggcaga ttgttctgta gctgcgtaga ctccttcttt tgaaatatca
    38761 aatccaacag cattttgaat ctttttcaat tttgaaagtt cattcaagaa gctaccttct
    38821 ccgcagccaa catctaatag cgagacttct tcaggtaaat gctgcgcaat agtctcaata
    38881 acgggctgat acatgccaga ttggatcatt ctacgccttg gaacgaacat tccttgatcg
    38941 tattctgttt taaccgcatg ggataaaaaa taaagtgttc cttttttgga aaggtcaaaa
    39001 cggtgattat ttttgcatat caagccatta tcagactttt ttacttcttc ttggcaaacc
    39061 ggacagcgga acagctgatg attttctgcc aaaaactggc gtcctcggtc aatttttttt
    39121 aacatagttc cccctcattt ctgttttatc ttaccgttag atgctcactt tggcaactgc
    39181 taaaaatatg atacaatgcc tagaagaaag tgaaagataa aggagaagat tagatgatca
    39241 aagaattttg cgctgaaaac tacacatcta ttcctctagc aattgctaga ggagccgatc
    39301 ggatcgaatt gtgtgacaac ttagcggtcg gtggcacaac cccgagtact ggtgtgatcg
    39361 aagaaacgtt aagttatgct ggggaaaaag atatccccgt aatgacgatt attcgtccta
    39421 gagggggcga cttcgtctat aacgatatcg aattgaaaat catgcatact gatttgattg
    39481 aagcaaagaa attagggacg gatggcgtag tatttggttg tcttactcct tcaggctggc
    39541 tagacgaaga ggccttagaa ctattgatcg aaacagcaga agggttgcag atcaccttcc
    39601 atatggcttt tgacagtatt cctgaagatc ggcaattcga agcaatcgat tggctggtag
    39661 aacatggtgt tgaccgtatc ttgactcatg gcggtcctgc tgacaaaact attgtagaaa
    39721 atttcccacg gcttaaagaa ttgattgcct atacagatgg ccgcttaatc attttgcctg
    39781 gtggtggtgt cactgcagag aatgcagcac atgtagctaa agaattgaac gtaagcgaag
    39841 tacacggaac caaaatcgtg gatcttcagt tataatctta taaacaaaaa aacatttgca
    39901 acaggcataa tgcttattgc aaatgttttt gtttttttac ataaaatagc ccataaataa
    39961 agaagcaata ttgaaataaa tcagtacact cgtcaaatcg ctcaaagtcg tgataaaggg
    40021 accgcttgct actgctggat caaatcctag acgatccatt agcatcggga ttaaactacc
    40081 tgcaagattc gccactgtga ttgcaaatag catagccatc cctatgacaa accccaagat
    40141 aaaattatgt ttccaaaaac ctacgattag aaaaatcgtg atccccgtaa cagctcctgt
    40201 gacaacgccg gtcaagactt cagccaaaac aagtcgaaca aatccatgat ctttctcatc
    40261 agaaatcgca agtcgacgaa cagccacagc tagcgactgg gttccagcat taccggcagt
    40321 tcctgtaatc agcgaaatga agactgctaa aatactcgct tcacttacca agtcttcata
    40381 gtgactgatt aaagaagcgg tcgacatacc taaaaacaac agagtgatca gccaaggcaa
    40441 acgtcgagac gctgccttta ctggattttc attaatttct tccacattga ccccggcaag
    40501 tcctgaataa tcgctagcag cttcatcatc aataacgtcg atgatatcat cgactgtgac
    40561 gattcctaac aaatgatcat cataatcggt aacaggaaga gctaagaagt cataatctct
    40621 aaatgtttgt gccacatctt cttggtcgtc gcctacatga actgaaagca ctcgttcgct
    40681 catcagatca gaaatcattg cgtcgtcatc attcacgatt aggtcgcgca gagaaataac
    40741 ccctactagc tgaccttctt gattaacgac atagatgtaa tagattgttt cagctacatc
    40801 tgcttcgttc ttcagcacgt acatcgcaga gcgaacagtt tgattggcta cgatcgaaac
    40861 aaattcagtt gtcatgatcg cgccagccgt ttcatcctcg taatggagaa gttccttgat
    40921 ttcactggca tcgtctgccg acatcaaact taaatattta gcaatttgct ttttatccag
    40981 cgtattaagc aagtcaacag cattatctgt atacatctca gaaagcatat cagctgcata
    41041 gctaggacgc atttcatcta ggtattcttt catatgttcg ttgtcttctt caatcacatc
    41101 gaacatatcc gctaattctt taggcgacag ataggaatag atcagctgcc tgttctcttc
    41161 tgtaagagat tggtagaatt gcccctgctc atagatatgc atctctaaaa aactgtttct
    41221 gaatgcctga atattatttt cccttaaatc ttgagtcaac tgggcaaatc tttcctctaa
    41281 ttcttgattt tcatccaaac caatctcacc tcgccttatt cttcaaaccg tctcacaact
    41341 gcctccatgt ctgcggctaa aggctgcttg aactccagca actcttctgt aaatggatgg
    41401 taaaaacgca gatcataaca atgaagggct tgtcttttta ttcccagatc cattcgcccg
    41461 ccatacatat catcaccgag cagggcacag ccaattgcgg aaaaatgaac tcttatttga
    41521 tgtgtccttc ctgtatgcag ctggatatca actaacgcga tttctgttag tcgcttctct
    41581 atccagtact cagtctgtgc cgaacgtcct tcatcagaga ccattcgctt caatagcgaa
    41641 gaataatctc gggcgatcgg caaatcgata tgtccatgtg ccgataaatc tgaaagcctt
    41701 ccactgacta aggcctgata ttttttcaca aacttctttt cacgaagttg catatctaat
    41761 tttgcgtgcg caaatccatg ctttgcaaaa agcatcaaac cagatgtatc ccgatctagt
    41821 cgcgtcacca catggattac ttggttttcg taaccttgtt ttttataata agcctttaca
    41881 cgattagcca ttgtcccatt tggatggtat tgggcaggaa tagacgaaac gcctgctggt
    41941 ttatttacta ccagaacgtg ttcatcttca tacacgatat cgatcggtgt ttcatccaaa
    42001 agtacggttt catgttctcc ttcagcagga atcacaatgg ttaccttatc atccttagct
    42061 agagaaaaca agacattttc tacttgatca ttaactttga tttgaccgcc ttgaaatttt
    42121 attttcgcta acagaccctt tgagatccct ttttctttta aaaaatattt tacttgctga
    42181 gaatgatctt tctcataatc ccatgtaaac tgcatatcaa tcctcactaa taaaggcatc
    42241 tttcacacga tgccaaaaat gcatatgtcg ataagaagca aaatggatcc gttcttctgc
    42301 tattcgataa taaattgagc ggatatttgc ttttgcgaca ttcaattgat caaccgttac
    42361 aagataatct tctgtatttt ccaattttat ttctagccat tcatcatggg caatcacgat
    42421 cggagaaccc aaagtacgaa acactcgatt attcagtgaa gcaatctctg ccaactgaaa
    42481 ggcattgata ctaggatgga tcacagcacc gccgacactt ttattatatg ctgtcgaacc
    42541 agtaggcgta gaaatcgtca atccatctcc tctaaaactt tcaaacagct cgtttttgat
    42601 gtatacatcg gctaccatcg tacgattggc ccgtttaatc gttgattcat tcagtgctaa
    42661 aaaatgctgg tccggtgttt catccaaata actgatccga acatccagca agggataact
    42721 aacactttgt tcccggttcg ttcgtaagct gtctactagt tcttccagct cgtaatcccg
    42781 ccaatccgtg taaaacccta aatgacctgt atgcacgccc aagaaacgca catcattcag
    42841 acaatgactg tagtgatgga acgccgacaa taatgtgccg tctccgccga ctgagatgac
    42901 caattctggc tcatgatcat ctcgttgaat ccctgcttgt tctaaaagtg cgttcaaacg
    42961 ttctgtcact tctatagtct ttgcttcttt attatgaacg atcgctactt tcatcccctg
    43021 cctcctctct ttcgctttct ttcttcaaaa gcgttcgata gtagacatca tctgattttc
    43081 ctcttccatg ggagaataac cgctgtgctt cttggatttc ttcacggata ctggacatct
    43141 cttcatccaa ctgataggca gcttctgccg cacgctgaag tcgttcactc atttcttctg
    43201 gaaaaacgcc ttgatattta taattcagcg aatgctcaat cgttgcccag aaattcatcg
    43261 caagagtccg aatctggatt tccgccaaaa ttttcttctc accgctgatc agctgtacag
    43321 gatactcgat caccagatga taagaacggt agccacttgc ttttttattt gtgatgtaat
    43381 ctcgttcttg gagaactttc atgtcttttc ttttacggat gatctcaaca acttgatgga
    43441 tatcttctac aaactgacac atgatcctta acccggcaat atcagacatt tcttcttcta
    43501 aacgtcccat tggaatatgc cgtaattgtg attttgttac aatactatcg actggcttga
    43561 ctctcccagt gacaaattca atcgggacat gtcgattttg ttcacggaac tgtttgcgga
    43621 tcccccgcag tttcactttc aattctgata cagtttgttc atatggtgct aaaaatgctt
    43681 cccaatctcg ttccatagct gatacctcta cttcattaac attcattgtt tattctacca
    43741 tatatgcaaa gttttttcgt actcttgatt tatgcattga ccaaaaaaag aaaattaggc
    43801 aaaatagaca aaaaggagtc gatcaaatga cacaaatgct tgaaatcgaa tataaatcta
    43861 tgctgacaaa gcaagagtac gagatattca tcgctcacta tcaattaaca gataaagatt
    43921 tccgtgtaca gactaatatt tatttcgata ccgttgatga acagctgaag aaacaaaatt
    43981 gtggcctacg gatccgtttc gttggtcatc aagctgaata tacattaaaa acacctgctg
    44041 aaaaagggcg tctcgaaaca acggatacct ttataacaga agaagcagaa gcatttatct
    44101 tcgaaaaacg tctgcctcgt tcaggcgcag tttttcaaaa actttctgat ttgaatatcg
    44161 atcctgcttc attaatccaa accggaaagc ttacaacaaa acgggctgaa ttccctattg
    44221 aagaaggact tttagctatt gatgaaagct ggggagacaa tctacacgat tttgaactgg
    44281 aactagaggt aggggatgca tctgtgggaa aaattgcttt tataaatttt ttaaaacgtt
    44341 tttctttacc ttatcgtcct gctaagaaca agatccaacg tatgcttgaa gctaaaaatt
    44401 agaaaccttt cattttcaca aggcacttga gttcattttt ttactttttt ctgttaagtt
    44461 ataggatgtg aaagaatcta gtcactagtt gtatagaatc tattttgggg gagattcata
    44521 tgatcgaaat ctatttattt gttaatcctt taggggaata ctgtttcgaa atggaacaac
    44581 agttattaca atttatcgaa gaagagtacg ggacaccatc aaaagaaaag atgcaatttc
    44641 gctttcttcc tttggtcaat ttgcagacaa ttggggatgt catgcaacaa aaaggaattt
    44701 ctcagaacaa tcttgaggaa cgtaatcact tattctcaat gacttattct gcagcattgg
    44761 actgcaaagc ggcgcagttt caaggaaaga aaaaaggccg ccgttttttg atcaaattgc
    44821 aagaagcagt cggctgtaat gacataccat attctcaaga acttgctgaa tccatatttg
    44881 aagaaatcgg cggcgatttg aatatgttta aagaagatcg tcagtctgac ttcgttaaag
    44941 aagtattctt ttctgatcaa aaaattgctc gggaaatggg catcacaaaa cacccttctg
    45001 cagtcgttta caattatgcc tgcgaccgtg attttggtgt tttagtagaa gtaaaaggta
    45061 caagtagcct ttggagaatc acagagctgt gtaaaacaac agataactca tatcaagttt
    45121 ttaatggtga acatttacat acaaatgatt ctcagttcca ttcaagaact agccatttac
    45181 acttgctttc taattaaaaa aatacgtcga ctcccttaca agagagccga cgtatttttt
    45241 tcgtttctat tcgagaaaat caaaaataga aagccttata aacgatcgat caattcttct
    45301 aattcattta gacgttgttc aaacatcgac attgcatctt caatatatgc agcctgtgtc
    45361 atatcaactc ctgctttttt cattacttcc acaggataat cgctgttacc tgcttttagg
    45421 taagccaagt agttctctag tgcctctggt tcctggttta gtattttctt cgccagtgct
    45481 gaagcagcag aaaagccagt cgagtattgg aaaacatagt aattataata aaaatgcggt
    45541 atccgtgacc attcgaattt aatttctggg tcttcttcga ctgctggacc ataatatttt
    45601 gcattcaatt taccataact atcacttagg tattcactag tcaatggcac acctttttca
    45661 tcttccgtat gcatgaaatg ctcgaattca gcgaattgtg tctggcggaa aactgttcct
    45721 ttaaacccat ccaagtaatg gttgagtaca taagcccgta cacgagggtc tttttctgtt
    45781 tccaataaat actccgttag gatattttca ttcgttgtcg aagcaatttc tgctaaaaag
    45841 atggagtagt cgccgtacac ataaggctgg ttcgaacgag tgaaataact atgaacacta
    45901 tgtcccattt cgtggacaag cgtaaatagc tgatccagcg tatcatgcca attcaataaa
    45961 atatatggat ttgtgtcata gcttcccgaa gaataagcat cgcttcgttt ccctttattt
    46021 tcgacaacat cgatccaacg ttcagagaat gctttttcta cgatggccat gtattcttca
    46081 cccattggtt tcagtgcttc taaagctttt tcttttgctt cttcgtacgt aaaggtaatt
    46141 ggagcttccc ctaagaccgg tgtataaaga tcatacatgt gcagtttttc cacttctaat
    46201 aaacgtttcc gtaattccat gtatcgatgt aacaaaggca aatgcttgtt taccacgtct
    46261 accaaagtat cgtatacact ttcaggaata tgattattgc tcaaagacgc ttctctggca
    46321 gagctgtaat tacggacttt cgctttaaaa ttatgtcctt ttatatgtgt gcctaaagta
    46381 gaagcaaatg tatttctaaa ttgttcgtaa acactgtaca atcccttaaa tgctgcttcg
    46441 cgcacccttc gatccgtgct ttctagcaac tgaccataca cgccatgaga taattggact
    46501 atttcaccat tttctccttc aatcgttgga aaaactagat ctgcattatt caaaacagca
    46561 aatgtatcac ttgaagcatc aaagatttca cctgctccag caagaagaga ttcctgctca
    46621 gcagataaga catgggctcg attatctact atttgctgga tataatggcg atagacttcc
    46681 aattttggtt cttctttgaa atattgccaa atctggtcat ctgacaattg caatatttct
    46741 ggttcaaacc aggaaacggc ttcactaact ttcgaaaaca gactgcttgc tcttgcataa
    46801 agcgcttggt aatctgtatt tccagtgtct tgatcgtttt taaggtgcgc atatacataa
    46861 atgacttcag tttggcgata aaccctcaat acgaattcaa tcgcatttaa aaattgagaa
    46921 gcgccttgat caagtgttcc tttgtgtttt tcagattgtt ttaactcttc tgataattcc
    46981 aaatatttct catcaaactc ttggtcgctt gaaaagatct tggtcaagtc ccaagttaaa
    47041 ttttcaggca attcttctcg ttttggcaac tgctttactt ccatgaaatc cactcctttt
    47101 cttcccttag ctctatctat caaaatagat catagtctaa catccacatg tcacagcatc
    47161 taacttttct actcattcat gtgtctgctt ccatcaattt tagcataact aaatcttgtt
    47221 ttacatagaa aaccgattcc tagcctattt ttatacttac tattttttca aagaagacaa
    47281 gtgtatgcat tcgttaaaaa actgctgata gaccatctct tcatcaatca taggaaaaag
    47341 ccattccctt ttatgttctt ttatttggca aagccatgag ttgaaatcag gaactttctg
    47401 gttttccaag gcttcttcgt acaacattcg ataaagaaaa acctgttctt gtaaataaaa
    47461 gaaaaaccga cttttttgat agatccacga tgatagatgc aaaagatgtt tatgacgcaa
    47521 gtagcattgt tcttgtatgg atactgtatt cttttgcttg tgaataagtt ggcgtgtcag
    47581 ccatttcttc ccatcttcac tcactttttc tatttttttc atttggttca ttttaatttc
    47641 gttactagtg aatatttctt taaggtccct actagaaaaa gtccagcagc tactgcaaaa
    47701 ggaaatatgc cctgatacag tctctgtaat atgatgatac aaataaagct tttgtttctt
    47761 gagatcagct ttccagcaat gcactcctct ttttctatta tagtagcagc aacttttttc
    47821 tatcaatgag aattgcgatt gtccaagaaa agaattacca agtatccacc atacttggta
    47881 actgttattc aaataatttt gggttctttc aataaatctc tggtgagaca gtctagaaca
    47941 ttggatttct attgctagat tcgccaacaa aatatccggc ctttgccgca acctcggcaa
    48001 ataggcttcc aaaaatactg gttctactga ctgtcccagc caatccatga agcattcctt
    48061 taactgtaag tgctcttttg attctctttc actaaagacc gaacagttat ctgatttttg
    48121 atgggcaaaa tgagcacatt ttttctttcc ctttttcaaa atcactcttt ttcgacagcc
    48181 acgacaataa tatttctttc ctttttctgc atgtttagca aaacagcact tattatattc
    48241 attcattgca agcagcattt ttctccctct gaacttattt acgtctaaga atttatttac
    48301 acgaaaaaag agggcgtgaa aaagctattc tgcatcaagt aataagaaca gctaaacaaa
    48361 aatagttccc aatatttttc gtttggttgg gcttgttacc gcagatgcaa gcttttgaac
    48421 acatttatac gaaaaaaaga ctgcgacaca atttttgtca cagtctctta ctataatgct
    48481 gatttataca aaataaaatt tcgttcgctc gatagcatta cgcgccatca gacattgtcc
    48541 atattcattc aatgtttctg gtgtacgatc gctcttttca gcaaactcca aaattcgagc
    48601 aatctgattc tctacatccg cttggttgag attttccatc cagaagtgaa cgactaggta
    48661 atagcgctct tcgaataaat acaaatcagt ccaagcagac tctagttcga catctgttgc
    48721 aagttggatc acttgttcaa aatcttcaaa agcaaaagtt gcttgtcttt cctgttcttc
    48781 tagggcgctt ccagataatt cttcctccgg atcgctgcca ataatctgct gacgaagaaa
    48841 gtcactcaca tcttctgaat caacagtaga atcttctgat tgagaaattt cttcatccgg
    48901 aagattttta ctgataaaca gttctaatcc gtctcctttt ggcaaaactt ggaaagtgac
    48961 agcttcgctt cctcggaact cctcttcgat atcaacttct tccaaaatac tataaaagaa
    49021 attttcgatt tctttgtgat tgccaagtaa atccaaaaat gaaacgcccc gttcctctaa
    49081 atcttcatga ccaattagta cacggatcgt attttcatta atatgttcca tttccatttt
    49141 gctacacctc actttgccac aatgtttaat atagattcat tgtagcgaaa tcagcttaaa
    49201 tgtaaagaaa aaaaatctcg ctgttaagcg aatttaataa aaccagcgga tttttcaacc
    49261 tttcaagcta tcttatttaa ctataacatt tttggcagac gaatactata tagccgactt
    49321 ttcagctaat tttccctctc tatttttcgt gcaatcatca tcttctacta cctttttata
    49381 catctaatca agaatacttt ttgtaaaaac aaaaaaactt ggaaaaacta tctttgtaat
    49441 agtcttttgg ctctatactt tttgatgttg gtgaacttca ccaacatctt ttttgtataa
    49501 aaaaatatag aaaagacacc ctgaaatcct ttagaattaa tttggctacc aattcatagg
    49561 aggattcaag atgtctcata ctcagcttat caaagatacc ctaaatattc tagacctaaa
    49621 tatctatttc gaggaaaatt gtttaactaa agaaaaatac aaagggcaaa tttgcttgat
    49681 ttacaaagga actcttctgt acaagccaga agagtgttcc cattgtcttt gtgtcgtccc
    49741 ctctaggatc attcgttggg ggacgacaac cgttcggttg ttactgaatg atgtgtctga
    49801 gtatcgcact tatctggagc tgaagaaaca acgatttaaa tgtaagtctt gccaaagaac
    49861 attcgtagcg gatacctctg ttgctgaaaa acactgtttc attagtcaaa aagttcgttg
    49921 gtctgtcatt gctcgcttaa aagagaatac ttccatgacg gaaatcgctc gtcagaaaaa
    49981 tatttccact tcttcggtct atcgtgtcat gaagcgattc taccgcccat tgaatccttt
    50041 caaacagact ttgccaaagg tcttatgctt tgatgaattc aaatcggttc gaagtgttgc
    50101 cagtgcaatg agttttatca tgatggacgg acaaagccat gcattattgg atattgtcga
    50161 aaatcgacgc ttgccttatt tagaacgcta tttttctcgg ttttcattag cgactcgtga
    50221 agccgttcaa ttgattgtta ttgatatgta tgcgccttat gtttcgttgg tgaaaaagct
    50281 tttcccgaat gctcagttga tcatcgatcg tttccacatc gtccaacata ttggcagaac
    50341 tttcttgaat catcgagtga aagaaaccac ggtacgactt aaaggaaatt cccactctca
    50401 acgagggtta gggaaaaaat tgaagcgcta ttggaaactc ctacaaaaag aagaagcaaa
    50461 gttaaactat gaaaaacggg tttggcgtgc cagttttaaa gattatttga ctgaaagtga
    50521 gattgccgat cgactgttag ctgcttgtcc caacttacgc caaggctatc agttatacca
    50581 agacattctt tatgcggtaa agaaaagaga tcagtcacta tttgaggatt gtttaactag
    50641 agacattagc ggtctgccag aaacctatac aaccacttta cgaacgttta aaaagtttct
    50701 gccacagatc caaaatgcgc ttcactacag ctattcaaac ggaccattgg aatgcttaaa
    50761 caaccacatc aaagtattaa aacgcaatgc gtacggcttt cgcaactttt acaatttcaa
    50821 actacgaatt atgattcgtc atggcaaaac atttttaacc aaataaacag aacagaaaga
    50881 ggttttcttc tttctgttct gtccaaatta ttctaagatt tctctttacc aacatcattt
    50941 gacaaagagc cagtcttttc caagtttcgt attatccttc tataatccag ccatcaattg
    51001 tgcttgacgt aattctaatt gtcgtacatc gcgcggtagg aaccgacgaa tttcatcttc
    51061 attaaaacca acttgcagac gtttatcatc aatcatgatt ggacggcgaa gcaatccagg
    51121 attgttttgc acaagttcca ataattcttg taacggcaga tcatctaaat ccatgttcag
    51181 tttttggaat actttggaac gtgtcgagat gatttcttct gttccgtctt ctgtcatttg
    51241 taaaatcgct tttaattcag aactgttcaa tggttctgaa aaaatattac gttcaacaaa
    51301 tggaatctga tgttcttgca accacgcacg agcctttctg caggaagtac aacttgggga
    51361 agtatataat gttaacaacg tgcaccactc cttttcgtga taagaatcac ttttttgtaa
    51421 cagaagatca taataatctc ccatctgttt ctattataca taatcaactt ttaaatagct
    51481 accttttttt cataattttt tta
//
LOCUS       DACDIG010000010        51107 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.010, whole genome shotgun sequence.
ACCESSION   DACDIG010000010 DACDIG010000000
VERSION     DACDIG010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.010"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            290..1135
                     /locus_tag="IV247_000670"
     CDS             290..1135
                     /locus_tag="IV247_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8776762.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1416..2636
                     /locus_tag="IV247_000671"
     CDS             1416..2636
                     /locus_tag="IV247_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAP8776763.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2633..5353
                     /locus_tag="IV247_000672"
     CDS             2633..5353
                     /locus_tag="IV247_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8776764.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5337..6281
                     /locus_tag="IV247_000673"
     CDS             5337..6281
                     /locus_tag="IV247_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8776765.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6350..7360)
                     /locus_tag="IV247_000674"
     CDS             complement(6350..7360)
                     /locus_tag="IV247_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8776766.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7540..7884)
                     /locus_tag="IV247_000675"
     CDS             complement(7540..7884)
                     /locus_tag="IV247_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP8776767.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7877..8311)
                     /locus_tag="IV247_000676"
     CDS             complement(7877..8311)
                     /locus_tag="IV247_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAP8776768.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8449..9186
                     /locus_tag="IV247_000677"
     CDS             8449..9186
                     /locus_tag="IV247_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8776769.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9179..10390
                     /locus_tag="IV247_000678"
     CDS             9179..10390
                     /locus_tag="IV247_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8776770.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10597..11376
                     /locus_tag="IV247_000679"
     CDS             10597..11376
                     /locus_tag="IV247_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAP8776771.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11446..12108
                     /gene="trmB"
                     /locus_tag="IV247_000680"
     CDS             11446..12108
                     /gene="trmB"
                     /locus_tag="IV247_000680"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAP8776772.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12206..13960)
                     /gene="ade"
                     /locus_tag="IV247_000681"
     CDS             complement(12206..13960)
                     /gene="ade"
                     /locus_tag="IV247_000681"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAP8776773.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14092..15021
                     /gene="cpaF"
                     /locus_tag="IV247_000682"
     CDS             14092..15021
                     /gene="cpaF"
                     /locus_tag="IV247_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAP8776774.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14978..16027
                     /locus_tag="IV247_000683"
     CDS             14978..16027
                     /locus_tag="IV247_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAP8776775.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16024..16302
                     /locus_tag="IV247_000684"
     CDS             16024..16302
                     /locus_tag="IV247_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAP8776776.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16277..16747
                     /locus_tag="IV247_000685"
     CDS             16277..16747
                     /locus_tag="IV247_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAP8776777.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16764..17066
                     /locus_tag="IV247_000686"
     CDS             16764..17066
                     /locus_tag="IV247_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8776778.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            17083..17454
                     /locus_tag="IV247_000687"
     CDS             17083..17454
                     /locus_tag="IV247_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAP8776779.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17426..17785
                     /locus_tag="IV247_000688"
     CDS             17426..17785
                     /locus_tag="IV247_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8776780.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17957..18964
                     /locus_tag="IV247_000689"
     CDS             17957..18964
                     /locus_tag="IV247_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8776781.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            18989..20173
                     /locus_tag="IV247_000690"
     CDS             18989..20173
                     /locus_tag="IV247_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAP8776782.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20610..22193)
                     /locus_tag="IV247_000691"
     CDS             complement(20610..22193)
                     /locus_tag="IV247_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8776783.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22771..22914)
                     /locus_tag="IV247_000692"
     CDS             complement(22771..22914)
                     /locus_tag="IV247_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAP8776784.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23136..23615)
                     /locus_tag="IV247_000693"
     CDS             complement(23136..23615)
                     /locus_tag="IV247_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP8776785.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23688..23972)
                     /locus_tag="IV247_000694"
     CDS             complement(23688..23972)
                     /locus_tag="IV247_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8776786.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24050..24244)
                     /gene="ssrS"
                     /locus_tag="IV247_000695"
     ncRNA           complement(24050..24244)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IV247_000695"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24316..25602)
                     /locus_tag="IV247_000696"
     CDS             complement(24316..25602)
                     /locus_tag="IV247_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAP8776787.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25740..26411
                     /locus_tag="IV247_000697"
     CDS             25740..26411
                     /locus_tag="IV247_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAP8776788.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26377..26865
                     /locus_tag="IV247_000698"
     CDS             26377..26865
                     /locus_tag="IV247_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAP8776789.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26878..27825
                     /gene="prmA"
                     /locus_tag="IV247_000699"
     CDS             26878..27825
                     /gene="prmA"
                     /locus_tag="IV247_000699"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAP8776790.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27826..28590
                     /locus_tag="IV247_000700"
     CDS             27826..28590
                     /locus_tag="IV247_000700"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAP8776791.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28823..31036
                     /locus_tag="IV247_000701"
     CDS             28823..31036
                     /locus_tag="IV247_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAP8776792.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31047..31493
                     /locus_tag="IV247_000702"
     CDS             31047..31493
                     /locus_tag="IV247_000702"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAP8776793.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31664..32923
                     /locus_tag="IV247_000703"
     CDS             31664..32923
                     /locus_tag="IV247_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAP8776794.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33012..33959)
                     /locus_tag="IV247_000704"
     CDS             complement(33012..33959)
                     /locus_tag="IV247_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP8776795.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34353..35645
                     /locus_tag="IV247_000705"
     CDS             34353..35645
                     /locus_tag="IV247_000705"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAP8776796.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35660..37429
                     /gene="aspS"
                     /locus_tag="IV247_000706"
     CDS             35660..37429
                     /gene="aspS"
                     /locus_tag="IV247_000706"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAP8776797.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37538..38413
                     /locus_tag="IV247_000707"
     CDS             37538..38413
                     /locus_tag="IV247_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8776798.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38679..40562
                     /locus_tag="IV247_000708"
     CDS             38679..40562
                     /locus_tag="IV247_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP8776799.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40637..40921)
                     /locus_tag="IV247_000709"
     CDS             complement(40637..40921)
                     /locus_tag="IV247_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP8776800.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40911..41645)
                     /locus_tag="IV247_000710"
     CDS             complement(40911..41645)
                     /locus_tag="IV247_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAP8776801.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41774..42397
                     /locus_tag="IV247_000711"
     CDS             41774..42397
                     /locus_tag="IV247_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAP8776802.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42589..43710
                     /locus_tag="IV247_000712"
     CDS             42589..43710
                     /locus_tag="IV247_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAP8776803.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43717..46845
                     /locus_tag="IV247_000713"
     CDS             43717..46845
                     /locus_tag="IV247_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAP8776804.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46864..50058
                     /locus_tag="IV247_000714"
     CDS             46864..50058
                     /locus_tag="IV247_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP8776805.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50204..50911)
                     /locus_tag="IV247_000715"
     CDS             complement(50204..50911)
                     /locus_tag="IV247_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP8776806.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16724 a         8936 c        10618 g        14829 t
ORIGIN      
        1 tttaaatctt ttgctaagtt gaactaatag agtcttttat gaatatcgaa gttagttttt
       61 cacctaactt cgttttttat agttggttat tataaaatag taatatagct gatttggatt
      121 caattcatat ttatttataa atcaaaaata cagagaatag tatataaatg tcctttccta
      181 ttcttaaaat gatgtttgta cttacttaaa ttaggtataa ttagtatatg ttttgtgaga
      241 aatttagact tacaataagc agtgaattta taatcaacgt ataggagaca tggatgaaat
      301 gaaactaatt ggtatagatt tagacggtac tcttttgaat tctgaccaga gcattagtca
      361 aaaaaatgca agaacaatga aacatttacc ggaagactgt tttccattca tttgttctgg
      421 aagagaagta gaagacatta ataatattct caaaaaatct ggtttaagga taccagcagt
      481 agggttaaat ggttctgttg gatttgatgg agaaaaaaag atatttgagt cttccttcga
      541 taaaaatgat gtaaaaaaaa ttgattctgt cctatcgaaa tttccaacca aaatatatac
      601 aaatctcgga agttatgaat ctcagaatta taaaaataaa atgaaaaaaa tttttcacga
      661 ggtagggagt gaattttcta tcaaagagct aaactatgag cttgagtatg aaaaatctat
      721 ttgttcaatc ccttataata atattgagga aattatagaa aaggatgcta tcagcattta
      781 caaatttttt atttttattc ctaatcgtca attgaaagct aatatattta accatttgaa
      841 aaagatgcta aatgtgtctg ttactgaatc ggcacctgta aatattgaaa tcgtacctca
      901 aaatgtatct aaaggtgtgg tgtttgatcg tttggaatca atctacaatt taaagaaacc
      961 tttgagaatc gcgatcggag atagtttaaa tgacttatct atgtttgaaa gtgcagatat
     1021 tagttttgca atgggaaata gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt
     1081 ttcaaatgat aaagacggag tatctcaagc atttgaaaga attcttacca tataaactaa
     1141 ttaaggtagt atagaacata gaattctaaa tacggaaaaa tggagcataa attccagtac
     1201 aaatagtact tcttcagtgt caaacgaagc gaatataaca tagatattta ggattgtgac
     1261 aaaaatcatg tcacaatctt tttttcgaat aaacttattt tttgaagtcg gccatttatc
     1321 gtaatttctt tgcataatac gaacaaattt tcggttattg atgaaaaaaa acattgaaaa
     1381 tggtatgata aaagaaaaaa aggaggaaat ttttaatggt gaaatttatc catgcggcag
     1441 atttacatat ggatcggtca tttgaaggac tgacaactct tgataaaact gtacaggaaa
     1501 aactattgaa gacgaatctg accgttttat caaatatcat tgaacaggca ataataaata
     1561 acgttgattt tgttttactt gcaggagata attttcatca aaatcgtcct tcattaaaaa
     1621 tacagaaaca tttttcagaa cagatgaaac ggttagaaaa gaaccatata cctgtattta
     1681 taatattcgg aaatcatgat ttctatcaaa aagagcggta ttggttctcc tttcctaaaa
     1741 atgtccattt atttacatcc gaaacggtag agacaaaaaa aataacgata aaaagtggag
     1801 aaactgtctc tctcagtgga tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga
     1861 tggaatttcc ttctagagaa ctcacagatt accatattgg gttgtaccac ggggaacctg
     1921 gcgtctctca aaaaggaaac tatgctcctt tccaaccctc aaagatgcaa gaaaagggat
     1981 acgattattg ggctttaggg catatccatg tgccaacggt cttaaatgaa aagcaaacaa
     2041 ttgtttatcc aggtgcgcct caaggtcata ctaaaaagga acaagcttct acatctattc
     2101 tactagttga attaagtaaa ggaagttgcc aaatacatcc gattaaagta gcagaggtct
     2161 attggaagac aaaggaaata tcattgagga aagcaagaac gacaaaagag gtcattgttc
     2221 acattcgtca gcagctttca aaagtagagg atggatttag tttaattgag ataaaattaa
     2281 aagattatga ccatctcaat acagatgttt tggaacggat acaatcagga gagctattag
     2341 actatttgtt agaggaattc tcagatagga taaatgattt ctttatttgg cgtatttctc
     2401 tcatagagaa tactttttat acgaagaccc cacttgctgc ttctgctaaa ttgatggaac
     2461 agttatttca atactatcag acacctcaag atttccaaca aattttaaat gaagtatata
     2521 gtcatcaaga agcggctaga atattgagcg aactgccgga atatcaagaa gaaacacttg
     2581 aaaaagccaa gcagctgctt aatcaagatt ttttatttga ggaggatcag gaatgaagat
     2641 tttaaggatc gagattgctg cttttggaaa atggcgtcaa aaaagttttg acttttattc
     2701 aggcaatcag ctgatctatg ggggaaacga agcaggaaaa tcaacgattt atcaatttat
     2761 tcaagcaata ttatttggtt tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa
     2821 agacggctca gcctatggcg gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc
     2881 tgtcgagcgc tacaagcagc aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt
     2941 gggaagtgat gagttactag aaaaactgat cgcacctttg acgaaagagt tattccagca
     3001 ggtatttact ttccagcaag agcagttgat ggaactagat catttgagag aaaaagagct
     3061 acatgatgcg ctgatttctt taggcatcac gggcagttct caagtattcc aaaagcggca
     3121 agaatattat cagaaagcac aacaattatt caagaaaaaa ggccaaaaat tactaatcaa
     3181 tcaaaaatta aatgaatgga aagagctgca agaaaaaatt catcaaaaac aagaacagga
     3241 agcttatttt ggcaagttga tccaacaaga acaggaatac gagtcgcaac agcagcagtt
     3301 attggcacaa ttaaaagagg cgaacaaaca attttcatta gccagacagc aagaacttaa
     3361 tttttcttta tatgaagaat ggcaatctct caaaaaaaac agcactacag gtcttcctga
     3421 tgagcaactt cttttagatc tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg
     3481 tttggaagaa ttagaagcaa cactggaaaa gcatagcggg atggatcagc agtcagccag
     3541 atattatttt tatttagagc aggagcagga actaaaagat ctccagcaac ttcaatttac
     3601 agcggaacaa ttaaacgaag aatggcaaag attacaaatt gagactagag aaacaaattc
     3661 agaattgaag attttggaac aaaggtggca ttggcagaaa aatccgttac cggaagaatt
     3721 tttatatgaa gaagaatgga agcatcttct ggctgaagaa agaaggattg ctgatctaga
     3781 aagccagatt tctgtgcgtc tccaaatagc taaagaacag caagagatga ttgaagcaga
     3841 agtgaatgac ttggaagaga cctatccagc attattacaa aatcagtcag agatagcacc
     3901 agtctcttca tctaaaccgt atatgctgat ggctggcgga gcaataatca ttgcaggtat
     3961 atttgtacca atgcccttga aagtgatcct tttactaatt ggtgcaggca tgattggcta
     4021 tttcttctac aaaaggcaac caaaaattaa aactaaaaat gatttctctg agataaaagg
     4081 aatgtggcaa gaaaaactag gacaacttga tctagcctta gctcaattag ctgaagcaga
     4141 agaaaaacaa aagagaactg gagaagaaaa acagattctt cagcaagaag tatatcgttt
     4201 tgcggatcat cggcacttag gaaagatgga tacgctcaaa ctcatgaaag aatatggaca
     4261 ggaagtcata gattatcaga aattatatgg cgtatttgag cggaaacata aaagagaaca
     4321 ggaaatacaa gaaaaactac gacaaatcga ccaaaaattt gagtttgtta aagaatggtt
     4381 accgatacaa gataagacaa tttcagaaaa aatggtattg ctttcccgtt ttatacaaga
     4441 gatggaacag atgaaatttg ccagaagcta tcagcaaaat actttattga agcaagaaat
     4501 caatcaattg aaaaagaaac aaaaagtatg gatacaagaa tacagcgaac aattgactca
     4561 agcaggctta tcttatcctt cggaagtacc tgtcttttta caaaaagcca aacaagccga
     4621 acagcagatg aaaagataca atgaattatc ggaaatgctt actccactat ttcctgaaga
     4681 gctgacaaaa gagaaattat ctgtaagact gcgccaactg aaagaaaagc aatcccaact
     4741 ccagcaagaa gaaaaagaag caagtgaaaa tcggcaacga ctccaacttc aagtagaaga
     4801 gttgcaaaga gacggcacat tggacgaact ttatcaacaa gctagccggc aaaaagcaaa
     4861 attacaggaa ttactggagg aatggggagc acttgaaatc gccgggacat tcttaggtga
     4921 tctttcaaca gagatgagtg aaagacaatt accccagttg ttaagagcag caggtgatta
     4981 tttaggtatt ttaacaaaag gaaattatca gaagatatta ctgaaaaatg atacactgac
     5041 tttaacagac ggatatacag aatttcccat ctatgagtta tctacaggta cgaaagatca
     5101 gctgattatg gcgatgagat ttgcttacct ctcattagaa gctaaccgtt cgatatgccc
     5161 gatcatcatt gatgatggct ggcttcatta tgatcatcag cgaaagtatc aattagctga
     5221 acttttagct aaatttggag aaaagtatca aataatttgt ttttcttctg ataaagaaat
     5281 ggtaagctat taccatgaga tgaaccaaat cgtaaaacaa ttagaaggag caaataatga
     5341 aaaaaattcg tgatttagta atcgatgagc agtttgaaca atttgtcctg atcaaaagtg
     5401 cagatgtaag aatcgctaaa aacggaaaaa aatttattgc ttttactttc caagatactt
     5461 caggtactat cgatggtaaa tactgggatg cttcggagga ggaaatcaat aaatttacag
     5521 ccggcacagt cgtttttcta aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa
     5581 agattcttca tatgcgctta acaagggtag atgagccagg agagcctagt ttatacatgg
     5641 aaagagcccc tttgaagaaa gaagaaatgg aagaagaaat caatcaattg ttgtttgaga
     5701 tcaccaatgc gcattggaat cgtattgttc gctatctact taataaatat caaaaacaat
     5761 tttttgagtt tccggcagca aaaagaaacc atcatgcatt tgccagtgga ttagcttatc
     5821 atactttgac aatgcttcac ttagggaaat caatcgccga tgaatatcct gagctgaatc
     5881 gagcattgtt gtatgctggt atcattttgc atgatttagg aaaggtgatt gaactttccg
     5941 gtcctatgtc gacagaatat acgcttgcag gaaatttgat cggtcatctg gtattgattg
     6001 acgaagaaat aactaaagcc tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc
     6061 ttgtattacg tcatatggtt ttagcacacc atggcttatt agaatatggc tcacctgtac
     6121 gtccaagaat aatggaagca gaaatattac atcaaatcga caatatcgat gcttcgatgc
     6181 agatgatgtt gacttcgatt cgtcaaacag aaccaggaaa atacaccgat cggattttcg
     6241 gtatggacaa ccgaagtttc tatgtaccaa aagatttata gcaaaaattt atttttttca
     6301 ttcaaaacaa aaaaagcgag acataagtct tcgctttttt tgttttcttt tattttgatg
     6361 aatcagttgt ttcttcagtt gaagattctt ttgttgaatc agttgatttt gtgtcagaac
     6421 tctttgttga agctgtagtt tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg
     6481 cactcaacac attttcaaag gcatcatctt tgatttttac atttgcatct tttaattctt
     6541 caccgattac tttagttgtg aatgcattgt cacttagttt agtttcagta gcgatatctt
     6601 ttagttgatc tttgtattta tccatgtcat tgcctttatt ttggtttttc accattttga
     6661 caacgtagta ttctgtcgca taagaagtag gatttgttgt agtaataaca tcggagatct
     6721 caccgtcttt caatttgaaa gctgcttctt ttacttctgc aggaatagtt gttgtcgttg
     6781 agtcgaattt aactttaccg ccatcttctt ttgtctctgt atcagttgat ttgtctttgg
     6841 ctaatttaga gaaatcatct ccatcatccg ctgatttttt cacatctttt gcttcatctt
     6901 cacttgataa tttgatgatt tgtgcttcta cttctggatg gaaagatttc caagcagttt
     6961 tcaaatcgtc atctgtaata tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt
     7021 tacgaatata ttctttataa gtatctttcg tatatccagc agcttctaat tgtgattcaa
     7081 aagtatcacc taatgatttc gcttgtttgt cgtattctgc atccacttgc ttatcggtta
     7141 ctttatcgcc atatttgtta ttgaatactt tataaatgat catccgttgt actaaggatt
     7201 ggttcgatga ttcaagtttt gcttcatcat agaaatcaga tacagtgatc gttccgcctt
     7261 tcatcgttgc gatatcttta ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca
     7321 acgcactagt agctgccaaa ataattgatt tttttttcat taaaatttgc actcctgtaa
     7381 ttagttatta tttttatata ttaactatac catatcccat ttcctttaaa aacgttgtcg
     7441 tggtagtttc aaacaaaatt caaaatttca tcacattcct aggaaatacg caaacttttc
     7501 tcacttatat agttaaatct gttgagaaaa taatcattat tatatttgtt caggatactc
     7561 tggcagatca gcttttagtt tttctacttg ttcattgatt tgtgtgatac gtggttcggc
     7621 ttggaattta aaatcttcga tatctttttt gattgcttct tggaaagcag gaatcacaga
     7681 ttcagctgtt tcttttgttt cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa
     7741 atcatttgtc agagttgttg cttcttccaa ttcttcgatc agtttctttc ttgtttcatt
     7801 cccgctcctg ggcgccaata acagacctcc gacagtgcca gcagcagctc caaataacag
     7861 tcctttgaaa aagtttttaa tcatcgcagg acacctgctt tacgattgtt tcttgaatag
     7921 cttttagttc ttccgatcca taactatctt gatgattgac aaagtgaata gaaaaatcat
     7981 cctctttgct gtagcgaggg actaaatgga tatgtgaatg aaaaacagat tgataggcta
     8041 gttcacggtt gttattcaag atattaagac ctttcatttc tggaaaagct ttttccaatg
     8101 cttgagcaat cttaggcaca cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa
     8161 aaatatctgc tacatgacgt ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga
     8221 tgtctaaaaa agcatagacc ttgtcgtctt catatatttt atagcttggg atttcttgat
     8281 tgatgatttt gcaaaaaata cagttttcca tataaaacat cctttctata aagaataaat
     8341 acttcagctt aactttacca taagttaaag ctattgtaag gaaaaaaaca aaataccgtc
     8401 attttcttcg tggtataata aataaaaata aatggcggag gaaaaagaat gagtttaatt
     8461 atcgatcatg tcacaggggg gtacggacat ctccctgtgt taaaagatat cagtttcgat
     8521 gtacagcctg gagagatagt agggttgatt ggactgaacg gagcagggaa aagtacgacc
     8581 atcaagcaca ttatcggtct gctggaagct tcaaaaggaa cgatagaaat caatcatcta
     8641 tccttaaaga aaaatacgga agaatatcgc aaacaaattg ggtatattcc agagactcct
     8701 tctttatatg aagaattgac attgagagaa catatcgaag tcactgcgat ggcctacgat
     8761 atacctatgg atatagcaat gaaaagagca gaaaagttgt tggagacttt tcggttatct
     8821 aatcgtcttg actggttccc agctaatttt tcaaagggaa tgaaacagaa agtgatgatc
     8881 ttatgcgcct ttttgatcga gccttcttta tatatcatcg acgaaccatt tttaggcttg
     8941 gatccactag ctatcaatgc attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc
     9001 ggtattttga tgtctacgca tattttagct actgcagaaa aatattgtga tcgttttgtg
     9061 gtcttacata acggagagat acgagcagac ggttctcttg agatgttgag aaaagaattt
     9121 cagctgccgg attcttcttt ggacgaaatc tatattgctt taacaaagga gcaggcacat
     9181 gagtagtttt tttcaaaaac gtttagcaag acatcaaaag aaaatgatga aatatatgcg
     9241 ttatgtattg aacgatcatt ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt
     9301 atattattct aattggttga aaaccctgca gccaccgttt gcaattggag ggctcataat
     9361 ctctgtattt tggatgctct gtttattcgt gggtaagatg gctacttttg cagaggcagc
     9421 agatattgtt tttttacttc ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt
     9481 tcgctattct tgcatttttc cgttgatctt tttgatattg gcatgcggat tggccatgcc
     9541 attagtggtg gttagtacgg agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt
     9601 atggagtttg aaattcagtc atctgcaaat caagagagca gaattgtttc gactagatca
     9661 acaagttatt cgttattgga aattaggctg gtttttttct tctctgatct ttttgtttct
     9721 atcattttat tggctaatat gggcaggaat agcaggtgca ttagtacagg caagtctata
     9781 ttactggttt ttgtggaagc aacaagaaaa aggattggat tgggagaaaa tggtgcatgt
     9841 agaagaacat aggttgcatc agatctatca atttatcaat ttattcacag atgttcctga
     9901 aattacggca aagatcaaaa gaagacgcta tttggatcct atattgactc atattaaaag
     9961 agaatcaaaa aacacttatc tttacttgtt tgcaagaaga ttggcaagag gttctgattt
    10021 tagtggtctt tatttccgat tagtaattat aggtgggtta ttgattgccg gagtagtcga
    10081 cttttatttt agtctgatta tcggtttctt atttttatat ttgatcggtt tccaactgtt
    10141 acctttagcg aaccagttcc gctatatggt tctcgttcag ctttatccaa tccataagaa
    10201 tcagaagaag caggcgattc agtatctact cacatgggta ttaattatcg cgtcggttat
    10261 ttttggtttg attgctgcag tcgtattaga tgggcagcag cgatgggttc cgctagcggc
    10321 ttatttgatt gttactatgc tctttgttaa actatatgta ccaactcgtt tgaagaagat
    10381 gtcagactga aaacaaagct gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca
    10441 gtaagaatgc tgttcgttct attttttttg agaaaagctg gttttttgaa tggcaattaa
    10501 atgaaaaaga acgtatatgt gcttgacgtg tagtgttcag gcgactagaa taataagtac
    10561 tgcaaaacaa agagtaagag gacgggagaa tgcatgatgg agtttcagtt agaccaagag
    10621 tggcggttac gtcccatcaa aggcgacaca ggaaaaacat acataggcat gcgtgatgag
    10681 gacaaagtct ttattaaacg aaacacgact ccgatgttgg ctgctctttc aagggaagga
    10741 attacaccaa aacttgtttg gacaaaacga acggggaatg gcgatacttt gacagcacaa
    10801 gagtggctgg aaggtcgctt gctgactcct aatgagattg gtcaaagaaa cgacgtgatt
    10861 gatgttttgt accatttaca tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt
    10921 ggtcgtgtca tgacgccgga gaagatgctg caaaaatatg aaaaagaatt accggaagaa
    10981 ctccagaaaa atcaatttct gcaaattgtc catcgttttc ttcgtgagca gatcccgtca
    11041 atagataagg aaaacttatc agtagtacac ggagatgtaa accatcgtaa ttggttagta
    11101 tgtaaaaatt atttatatct agtcgattgg gattcaatca tgtttgcaga ccctgcggtg
    11161 gatattgcaa cgattttagg aaattatgtg ccactgtcta gctggaatca gtggctgatc
    11221 agttatggca tacgcccgac tgacgaaatg cttgaaaaat ttcattggta tgcggccatg
    11281 aatttgctcc aagaaatcac cagatattgc aaacgtgaag atcatcgacg gatgaacgaa
    11341 gaaattttac aattaaaacg aattttcagt ggctgaggtt tggcccaaac tctgcggaga
    11401 tttggccaga cctttttaat tagaggaaag gaatggagat aaataatgcg tgtccgaaat
    11461 cgtccgggag cagcggaaat gttagcggct cacccgaatt ttgtaattag tgatccaaca
    11521 ttgtggaagg gaaaatggaa tgaattgttt gaaaacgatc atccaatcca cattgagatt
    11581 ggtatgggga aaggccagtt catcactgga atggcaaaag cccatcctga aatcaattat
    11641 attggagtag aaatgcaggt cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa
    11701 ccgcttccta acctgaaact tctacatgtg gatggatcag cgttgacaga gtatttcgct
    11761 gacagcgaag tagatcaaat ctatctgaat ttttccgatc catggccaaa aaaacgtcat
    11821 gaaaaacggc gattgacata taagactttt cttgctgtag atgaacagat tcttcgtcca
    11881 aatggagaaa tccatttcaa aacagataat caaggattgt ttgagtattc tctggcaagt
    11941 ttttctcaat atggcatgat cctcaaacag gtatggttag atttacatca aagtcaattt
    12001 gaaggcaata ttatgacgga gtatgaagaa aaattttctt caaaaggtca acgtatttat
    12061 cgagtagaag ctcaatttca agataagacc aaaaagaaaa gtctgtgaaa ctaactttct
    12121 gctttcataa aaacattaat atccataaat aaatctcaga aaatgaacat tacagatgta
    12181 atgtattttc tgggatttat ttgatttaaa atgtatttat ttcatcggtt tttccacaag
    12241 gtggaatcaa ttggaagcga gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg
    12301 gtatgacaga taatgttaag aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc
    12361 ctagttgatg tgctttagtt attaattcat tcaactgttc atagacgatc gaaaaaggtt
    12421 cctgtgtcat gattccgcct ataggaagag aaacacaagc tatttcctgc tgtttgttga
    12481 caacggtcat cccgccacct ttttgaatca aaagattagc agcatacagc atatcctcgt
    12541 cgtttgtccc aacgacaact agattatgcg agtcatgggc aatcgttgtc gctatcgctc
    12601 cttgtttcag ctgaaatcct ttcactatgc ctaatccaat gtttcccgtt tgatggtgac
    12661 gctcgatcac cgcaatcttt aattggtctt tttcgattga aggaatgaat cgttcgcctt
    12721 gtcggtccac tttttctacc aaatctttag tcactagact attcgggata atttcgatca
    12781 catgggcttg tgaagaagaa agaagcagct ctaacgaatg tggatttaat ggttgagcgt
    12841 ttagttttgg cagacaacac gtatatgaat ttgaatgatc agcaaataaa tggtcttgga
    12901 gttgcccaga ttgaacaact aatgttccct tatagaaaac ttcatgaatt ggcagcttgg
    12961 taagatcgtc tgttaagaag aaatcagctt gatacccagg ggcaatcgca cctagtgttt
    13021 tcaaatcaaa acattctgct gcattcaatg ttgccatctg aatggcttgt aatggatcga
    13081 ttccaagtgc aatagcttga cggacgatat gatcgattga tccttctttt actaaatcat
    13141 tgatcagttt atcatcggtg acaaagaagc atcttcttga attttcaggt gtgatcgccg
    13201 gtagtagcga tgccagatct tttgcaacgg tcccttctcg aaccattaaa tacataccaa
    13261 gatccaaacg ttctttagct tcttttgcag ttgtagcttc atgatccgtc cgaatacctg
    13321 ctgcaggata aacatttaac tcttccatgc cgataccagc tgcatgcccg tcaatttttt
    13381 tcttatgctg gtgcatcaat tggatctttt ctaggatgtc tgtttcatta tttgctactg
    13441 cttgatagtt catgacttca gctaagccta aaacttcggg tttttgtaaa aaaggggcta
    13501 atttttttgc atttaaattt gcaccattgg tctcaaaagg ggttacaggt acacaagaag
    13561 gtagcataaa aaagatattc atgagtgttt gtcttgcatc ttctatcata aactcaagac
    13621 ctgtactccc agcaatgttt cctatttcat ggggatctgt cactacagta gtaactccat
    13681 gaagcaagga tacttttgaa aattcgcttg gtgataaaag tgagctttcg atatgcacat
    13741 ggccatcgat gaatcctggc actagatatt gcccattagc attatgtacg ctttttcctt
    13801 cgtattcacc gattcctacg atcattcctt cgcagattgc tacatctttt tccatgattt
    13861 ctttcgtaaa gacgttgacg atcttagcat gcttgatcac taggtcagca ggcttttttt
    13921 tacttgctac ttcgatatat ttccttaatg ttgtttccaa tggagtgcct cccaagataa
    13981 atttttataa gtataacata aattcggctt ttgccaatat ttttttgtaa attcatttta
    14041 tttgaaacaa aaagatcaaa aagacgtatg ttttttgtgg aggggagaat aatggaaata
    14101 aaacgattat cggatcaaat gataagtgaa agttttgcca aacatgccca agacgtatat
    14161 atattgccag cttcagaagt attttacatc tattttcgct atggaaaaaa acgaacattc
    14221 tggaagaaag tctcttcggc agatggacag caactgatct caagatttaa gtatttggga
    14281 cagatggata tcggagaaaa aagaaaagct cagttaggtg catttactta ttctttagaa
    14341 aaacaggaag ttcgtctgcg gttgtcttct gtaggagatt accttcaacg agagagttta
    14401 gtgattcggc tattatacgg tacttttgaa acaccccatc gttgctttag ttctcgagat
    14461 tttgcaacta ttttacagaa aaccgtcaga cgtggattat accttttcag cggaccagtc
    14521 ggatcaggca agacttctct aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt
    14581 actatcgaag atcctgtaga gatagaagaa ccgttttttc tgcagctaca gatcaatccg
    14641 aaaatagcac agacttatga tacacttttg aaactcgcat tacggcatcg tccagacttg
    14701 ctaatcattg gcgagataag agatgaatca actgcacatg ctgcggtaag agcagcacta
    14761 acagggcacc gggtttttgc aaccgtacat gcacgcaatc ttgaaggtac attggtcaga
    14821 atccatgaac tgacaaagaa aaaagaagag ctatctgaat gtttagctgg tgtcatttat
    14881 caagaacttt tggaagatgc caatcaccat ccttctgttt tatggagtta tcaatttatg
    14941 gaggaagaat cagtaaaaaa atcatgggag gaaagttatg aagaagctca ggaaaatatt
    15001 tggcagtcag aactattcta aaggattaac aagaagccag caaaaccagt ttgcacaaac
    15061 catgggagaa ctgcttttat ctggttttag tctgcaagaa tccctttatt tattactcac
    15121 gatccgttcg tttccacctg ttattttggc aaatatccaa catcagctaa aggaagggca
    15181 agcgtttagt gaggtacttt cgcaaatagg atatggagca gagcaactgg tacagatcga
    15241 gttagctgaa cgtcatggga atctgccgac aactttgttg gaaattgcca aacagtttcg
    15301 gcttgttgaa ggatttcgca atgatttgaa aaagctgctt gcatatccga tgctattgct
    15361 cacctttttg ctaggtatct tgtttagtat gcggttgttc gttttgcctc agcttattgg
    15421 ttcaggaatg gtcatgcaag agcattgggg tattcagctg atacaagtct ttcattggta
    15481 tgttctacta ttcatcctag cggttactct attggcttta ctagtaaggt atcgtctgat
    15541 gaagttgtct cctgttgatc agagtgaatg gataagcaga caattattga ttggaccgat
    15601 atattccttg tatcaatcgg cttatttgtc actagagatt ggaaagcttt ttcatgaagg
    15661 tttcgaatta aggcaaatca tagtctgctt aaaggaaacg aaacaagaaa gtctgattca
    15721 gcatcttgcc tctaagttga ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt
    15781 ccagaattat cgttttctat cagaagaatt cagtaaaatt gttttacagg gagaggcaaa
    15841 gggaagttta ggtaaagaac tgatttttta cagcgaatgg acaagaaaac agcttttcaa
    15901 acgtcttcat cactggatgc attttattca gccaatcatt tttttgggcg tagcggcttt
    15961 gattttatta gtttatgccg ctgttttatt acctgtttac ggaagcatag aggaggtttt
    16021 gccatgaaaa agagaaaaat atcttattat agcggtttta cattgatcga aatgctaatc
    16081 gtcttactga tcatcagtgt attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg
    16141 aatcatgtag accaagaaag tcgtgaagcg atcatccagt tggtagacac acaaaaagag
    16201 ctctatgcgc ttcaaaataa tggaagagtt cctactgtgg aggaattgtt gaatgaaggc
    16261 tatatcaaac gagaacatgc agaaatctat cagcggcctt gaaggtaagg ttttagcatc
    16321 aggatatacg ttgtttgaat cactcttact gattttatta cttacaacga tcctttcttt
    16381 tccagttctc gcattttctg catggaagaa tgaattggca gtctatcaat ttttcggcca
    16441 atttgaaaaa cgaatctatt ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg
    16501 cttttattgg aatagtgaag agaatcaaat catttttcat gttccgaatc cagataaaat
    16561 aaattgggaa gtattagata ttcctgatga gattaccttg aaacgccatg cttctattac
    16621 ttttgctgct agaaccggga atgaaagttc actaaaagct tatcaatttt attgggaaga
    16681 aaaagagcgg acgattacgt atcaattcca gatagggagt ggtcgctata caaaaagaat
    16741 tgaataaaac aggatttgtt ttgttggagt cccttacagc gcttatgatc gcttctgtct
    16801 gtgtctactt agtgagcttt ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg
    16861 aggaactgct atttattcga atgatttatg aagatatcaa gacgcagcgg atgtatcaca
    16921 aaagaccaga gcgaagggaa gaaatgatcc catacgatat ccaaattgtc gataataatg
    16981 gagtaatcga aaaagtgttt gtcaatatgg agaaagaagc gatcagtatt gaaaaaacaa
    17041 gtaattccag catttacgct gattgaatgt ctaattggac tgatgatatt gagcagtcta
    17101 ttgctcacat tcaatctttt gattcagcaa accatccaag taagtgaatt tttgcagcgt
    17161 aaagatcaaa aagagtggct aatattttta gcacaattag aagaagaact caagagcagt
    17221 cacaatgtct ctgtgaaaaa tcagcaactg cactacacga tcgaggataa acagtatatc
    17281 atcgaaagat atcaggcaat gatacgtaaa agaagaactt ccggcggaca tcaacctatg
    17341 ttaacaagta tcactgagct tcaattattg gaaaaagaac acacaatttc tttttatgta
    17401 cactttgaaa atggagaaga tggatatgcc atatggacaa aaaacgatca gtagaaaatg
    17461 ttataagggc tctattttat tttctgtctt gcttttattt cttttatgta cgtttttatt
    17521 gatggcagca ttagaaagct accgtttgtc aatggatctt aatatacgaa tcaaaaatta
    17581 ttatatagca aaaataatga agcagatggc tttatcggaa cagtctacgc ttgccgaaag
    17641 ttcggaagga gttttttact acacaactgg atctgtaaca tatcagtggg tacaacagtc
    17701 cctattctta gaggtggaag tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa
    17761 tgatacagat actagtacag aatgatggaa tgttcgtgaa aacgccctct tttctgttat
    17821 aacactgttt ttaataaaaa aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat
    17881 tgaaattttg tcaattgttc gatagaatta aacaggtgaa aaaattgtta taatatatct
    17941 agaaatgagg tgcaccatgt taccagaaaa aatggaacaa gggtttaatc aaaacctgga
    18001 agctattcag cttttacaga atgcattggg aacttccttt ttagaagcct atgtcgaaaa
    18061 tgcagaaaat ttaatagatg attatcaagt acgtgtggtg gatggagtgc cgacaaaaga
    18121 gacaacgcaa cggatcacag ctctttatga agagttgaag aaactttctt tcgagccaga
    18181 agaatggcgc agactgtctc aattactttt attgaagggc agccagacag aacatttgca
    18241 ggcgaaccat caactcactc ctgatagtat cggtttttta tttgtttttc ttattgaaca
    18301 actatacaca aataaaaaag caccggttaa gattctggat attgcggcag gaatgggcaa
    18361 cttgttatta acagttcttt taaacctatc gaatgcgggg taccaaacag aaggaattgg
    18421 tgtggatatt gatgatacat tgttggctgt cgcagcttct acaagtgatt tgacacaagc
    18481 aaatgttcaa tactttcatc aagacgggtt gcaggaatta ttaatagatc ctgtcgattt
    18541 tgcaatcagc gatttgccaa tcggttatta tccgaatgac gaaaaagcaa aagagttttt
    18601 aacaagtacc gaagaaggcc atagttatgc acatcattta ttgctagagc aatcaatgaa
    18661 atatgtgaag ccggatggtt ttggtttgtt tttgatgccg agtggatttt tagaaacgga
    18721 tcaaagtgaa gaaatcaaaa aatggtttaa agaagaaggt tatcttcaag gaatgattca
    18781 attgccagat gaattgttcc gtaataaaca atcgcaaaaa agtattttga tcttacagaa
    18841 aaaaggacca caagcacaac aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa
    18901 agaaccagag aaggtgacgg aatttttcaa cgagtttaaa aactggaaat catctgctct
    18961 gtaatcattc aacgagaagg agagtcatat gtctaaaaca attgcaatca atgctgggag
    19021 ttcaagtttg aaatggcaat tatatcaaat gccagaagaa accgtagttg cgaaagggat
    19081 cgtcgaacgt atcggtttac aagattctat ttttaccatc aagtacggcg ataatcaaaa
    19141 attcgaacaa atcatggata tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca
    19201 gttgattgaa ctgcaaattt taagctctta tgatgaaatc accggagtag ggcaccgtgt
    19261 tgttcacgga ggagaaactt atggggattc agtagtgatc gatgagaaag taatggaccg
    19321 cattgctgaa ttagcagaat ttgctccttt gcataatcct gcaaacttaa tggggatcaa
    19381 agcgtttaaa aagattttac ctgacatttt gagtgtggct gtattcgata catctttcca
    19441 ctcaacaatg ccaaagcaaa attatttata tagcttaccg gtgtcttatt atgaagattt
    19501 caaagtaaga aaatatggct tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc
    19561 aaaattattg aacaaaccga tcgaagaact aaaaatcatc acttgccact taggaaatgg
    19621 tgtatcgatc acagcagtcg atggaggtaa atccatggat acttctatgg gattcacacc
    19681 attagctggg gtgacaatgg gtacacgttc aggtgatatc gatccagctg ttctcccata
    19741 tttaatggaa aaattagaga ttgatattga tgaaatgatc aatgttttga acaagaaatc
    19801 tggtctgcta ggcctaacag gattgtctag tgatatgcgt gatcttgaaa agaactatga
    19861 aaaagaacat attcgtttag cttatgacat tttcgtggac cgcatccgaa aatacatcgg
    19921 cggttatgtg acaacgatga atggtgttga tgcaattgtc tttactgctg gtatcggcga
    19981 gaatgacgga catgtacgta gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat
    20041 cgatccagcg ttgaatgaat tgcgtggtga agaagtagat atctctacaa aagaatcaaa
    20101 agtaagagta ttagtgatcc caacagatga ggaactaatg attgcccgtg atgtggagcg
    20161 tctgcgcggc taagccaaag cattcgttag gataaaagat gaaacgcatg caagagggaa
    20221 ttcttgcatg tgtttttttt gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc
    20281 cttctttttt acaggatctt cttagggaat tttgcttgga aaagtggtca aaagggaaaa
    20341 gactggcatt ttatttattg attttcagga aaatcccaat attttatttt cattcatctt
    20401 tataagggac attacatatg taaaaagaaa aaacattttt agatcagcct cgtgggctgt
    20461 cgactttatt cttcgcttta tgtttagcta gaaaaccgat ccgaaacatt cattgatcga
    20521 ataataaaaa cttgattttc cacctgtact ttgcctgaat cgtgaaagca caggtatgaa
    20581 aatcaagttt tttgttgcga aaaaacgtcc tagtcaatca gttggccgag cggctggcgg
    20641 ctgatctgct cctgatcaat aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg
    20701 tcatggctga ctgctaggag cgttccttga tagtcttgca gcatttcttc aatttcttct
    20761 cgagcatagc tgtcgaggtg attggttggt tcatccaaaa tcaggacgtt gattttttct
    20821 aaaaagagtt tcagcaacgc caaacggatt ttttcaccac cggataaatc tttgatccgt
    20881 ttacccacat cgctttgata aaagccactt ttggctaatt gccgtcgagc gacttcttcg
    20941 tttccagtca atgtgatggc gtaagccagc aaacggctat cgggatcggc aaactgcagc
    21001 tgctgcggca aataaccgat ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc
    21061 ttttcctcac cgagtaataa tttgagtaag gttgattttc ctgcaccatt gtcgccgatg
    21121 atggctaaat gatctccacg atagactgca aaactgctgt ctttgaatag tacttgatca
    21181 gcgtactgct ggtgcaagtt ttgaatgatg aaaacttctt tgccagattt tccaccatta
    21241 gacaagcttt gcagccgatt tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc
    21301 aggcggcgtt ccagttcttt ggcttttttg aagaaggact cattgtctcc ttcatgtgcc
    21361 cactgacgaa aacgacgaat catgactttc aaccgctgga cttctttttg ctgcagttcg
    21421 taattttttg tcagctcagc caagcgagct tctttcaaga ctcgaaaacg gctgtagttt
    21481 ccgggatact caatcaatgc accttcttcc agttcccatg tttttgtggt gacttgatcg
    21541 agaaattgcc gatcatgaga aacgaccaga accgattttt ttgtattctg taaatagttt
    21601 tccaaccagc gaatactgtt aagatctaaa tgattggtcg gttcatcgag taaaagtaaa
    21661 tcggcatcgg ctgctaaaat tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt
    21721 tctgttaacc gtgcttcctg acggtgtgga atccctaagc cttgcaagat cgctgcaatc
    21781 cggtcttcta atacgtaacc gcctagttct tcaaagcgat gctgcagctc accgtaacgg
    21841 gccagacact tctctaagtt tgtatctggt tctgtcatca atgcttcgta gcgactcatt
    21901 tgctgtttga ggtcctgtat ctcagcggca gaatggagca agtagtggtt gacggtcgct
    21961 tcggaaactg taagttcttg ttcgaccgtc ccgatcttta agcctttttt gcgactgata
    22021 acaccgctgt cgacaccttc tctgccggtc aagatttgta aaaaggttga tttgccagtt
    22081 ccgttgatac cgactaaggc aattttttca ccagcttcaa tttgacagga taaatcctca
    22141 aataatggat tcccgccgat tgttttttta atagaaattg cttgtatgat catgatctct
    22201 ctccttgaga ccactcaaaa aggccatggt tatccctggc ctgtgttatt taaactcaga
    22261 ttatcatgag aaaaatacct tgagatggcc attgatttta cgtgaattca actgttttgg
    22321 acaaacttgt cccgtcaaca gcaactgtaa aaccaattgc atggctaaat gcgatatggt
    22381 atcgttggta attttcatta cttgttattt tagtcatgcg catcatagta ttttccctcc
    22441 ttcgtgtagt tgtcttttcc agtgtacctg aaagtgtcag aaagttcaac tagcgctgac
    22501 atttacttaa tatttatcga tgtgtaattc tgtcagagag ctttttttat ctgactgagg
    22561 gtaatgtctc gtggaccaca tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca
    22621 tatggtgttt gataaaaaac tttgtctaca tgacttcctt ttgccatttg cttaataccg
    22681 ttgcattaaa cagctcttct ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt
    22741 gtggttgcaa tggcaagcgg tagcctttca tcaattcttg aatgatttac cgaatgtagc
    22801 acttgccttc tcgcggtacc cgaacccgaa ataaaatttt agtttggcga taaaatcggt
    22861 atccgccatc gatacttcat attttttcgt atcagattca tcggaaactt ccacattgac
    22921 gaaccaataa gctttggcat gcttaggtcg ttttaaacca caccagggcg actaatcgcc
    22981 ctggtgttca ctttttttat tttacttgaa ctcattgact gttttccctt tattttaaac
    23041 attataaaga ccgatgagtg cttgccgcgt cttttgtttt tatcacggtt ttttgtgcaa
    23101 aaaaacctgc cgatagttgg caggtcgtct acgtgttaag ctttggtgtg gtctttagct
    23161 ggcaattggt tttctaaatc agttcggaca acaagcacat cgcaagctgc gtttctaata
    23221 acatattcag aaacagaacc aataaataaa cgttctacag cattcaatcc tgtggctcct
    23281 aacataatca aatctacttg attatcttct gggatttctc tggcaataat ttgttttggt
    23341 gagccatatt ctacgacaga ggttacatta ttcaagccag ctttcttcgc attgctttca
    23401 taatctgcca atgtttgctt tgccatttca gtagcttgtt ctgcaagcat tccatcaaag
    23461 gatgaaacgg tttggaaaga acgtgtgtca atgacatgtg ctaatagaag ttctccgtta
    23521 tttcggatag ctacattgac cgccttttta aaagctaatt cagcttcatc agaaccatct
    23581 acggcaacca tgattttttt gtattgttgt aacataagca ttcactcctc aagtcaatcg
    23641 tattgtactc ttttattgta gaacacttta gggaaaaaga gaattattta ctgctttggt
    23701 tcagcaattt tttttgcaaa tcggatactg aaggtggcag aaacaataat tacgatcagt
    23761 aaaaagacca aggcaataaa gcgttgattg aaaaaagctg ttaaaatacc cattacgcct
    23821 aaaacagcat atgcagcacc gtaaaattgt aggaatcctt gatttttatc atttttgttg
    23881 atcaatacaa aaaagatatc ctgtttatta aataaataat agctaacgaa taataatact
    23941 aaagtggcga taatcattaa aatcctaatc atgaacaaat cctccaaaat aagcaacaaa
    24001 tagaaaaagc aatttgccaa gagcaaattg ctttattctt taaaagatgg taatctccgt
    24061 taatgccgtt gtttgtcctg tagtattgat cccgcgaatt caagcaggtg ggtcccatgg
    24121 tcgtagcttc gaactaccaa atgataaggc atgttaccag cagtgacaca agacgggtcc
    24181 caagatatca ataaaaatgt tcggtcaaca cacaaagagg acaattcgaa catcacagat
    24241 ttcccacctc tatagtagca tatgaattta aaaagagcaa tgagagagct cgcagaaaca
    24301 gaaccgtttt cttgtctagt tttttggagg atgctttttc cactctttta tacggtaata
    24361 ctgttgaccc agagcctgtt catatttccc tgtatctttt ggctgataat actgagcatt
    24421 cttcagttta tctggtaaat attgctgatt gacccaagcc tgatcaaaat gatgaggata
    24481 ctgataaccc acgccacggt tgagtgattt cgcacctttg taatggctat ctctcaaatg
    24541 atcaggaaca tctccagctt tgccctctcg aatatcagca agtgctgcat ctaatgccat
    24601 gtaggcagaa ttggattttg gcgacaagca aagatcgacg acgacgtctg ctagtgggat
    24661 acgtgcctca ggcaatccca atttttcagc agctaataca gcgttcactg tacgagccgc
    24721 agcagcagga ttacctaaac ctatatcttc ataaccgata accatcagac gccggcaaat
    24781 acttgccaga tcacctgcct caactaaacg ggcgagataa tgaagtgcag catccacatc
    24841 acttccacga atcgattttt gaaaagccga aataacgtca taatgagcat ctccattttt
    24901 gtcatgggta agagcttttc tttggacaca ttcttcaatg atcgacagtg taagatgaat
    24961 ccttccttct ttatctgaag gagtcgatag tgtagccagt tccagcccat tcaatgcact
    25021 ccgcaaatcg ccgttcgttg cacgcgaaag gtgaagaaga gcatcttcgt ctaactggat
    25081 tgcttgttgt ccaaggccgc gttctttatc ccttaaagca tgttcgactg ccaactggat
    25141 gtcttgttct gttaatggtt tgacttcaaa gatctgtgta cggctgcgga tcgctggatt
    25201 gatggtaata taaggattct cagtagtcgc tccgatcaag atgattcggc cactttctaa
    25261 atgaggaagc agaaaatcct gttttgtttt atctaatcgg tgtacttcat ccaataagag
    25321 gatcactgta ccactcattt ttgcttcttc cgctacaact tgtagatcct tttttgaatc
    25381 tgtagcggcg tttagcatgc gaaatgcata attagtagag ccggcgatcg cacttgcgat
    25441 actcgttttt ccagtaccag gaggtccata tagaatcatg gaagagagca tgcgagcatc
    25501 gaccatgcgg cgtatgattt ttccttcccc gactagatgc tgctgaccaa cgacttcatc
    25561 gatcgttcga ggtctcatgc gataagccaa tggctgttgc ataggatttt cctccttctt
    25621 gtttttacgt cccttaatta tatcatattt agcaagatgc ctagaaagaa cagccttctg
    25681 ttgttttgtt ttcatagctt atccaactaa tattgtgtat gataaagtag gtgagaaaaa
    25741 tgacggataa acaaaagata gatgaatttt tttggaataa aacgacgcca gaagtggcac
    25801 gggatttatt ggggatgtat ttggagcatg acacaccaga aggaagatta gctggatata
    25861 ttgtagacgc ggaagcatac ctaggtccag aagacgaggc cgcccatagc tatggtttaa
    25921 gaagaacacc gcgtgtgcgg gcaatgtatg aaaaaccagg tacgatctac ctttacacga
    25981 tgcatactca tcggattttg aacatcatta ctcagccaga agggattcct caaggagtaa
    26041 tgatacgtgc aatcgaaccc gccgcaatga ttgatcaaat gagtaaaaat cgtggcggaa
    26101 aaactggtcc agatatcagc aacggtccgg gaaaattagt ggaagcatta gcaattcctc
    26161 aagaacttta tggtcagtct atagcagata gttcgttacg cttagttttt gagaaaaaaa
    26221 agacaccaaa aaagatcaat gctttgccta gaatcggtat cccaaacaaa ggtgtatgga
    26281 cagaaaagcc gttgcgtttt gttgtgtcgg gaaatcctta tatctcttta caaagaaaaa
    26341 accaaatcga gaaagattgg ggttggagga aagaaaatga aaaagaagga tctattaaca
    26401 tatttagatg aaataattga aaaacaagtg acagactatg atgtagcttt agattgggat
    26461 acaaaaaatc atacgatcga gatcgttgtt cgcttgtttg cagaaaataa agcacaattt
    26521 gagattgatg atgcagaagg gatcgtttcg caagaaccaa ttatcgaatt tgaagacggc
    26581 gtacttcttt ttaatcctca gaaatctgtt tttgatgaac gcaattatct agcagtaatc
    26641 ccttatgaag gaaaaaaagg gttggcaaaa tcagtagctg attctttagt ggaatatcta
    26701 aatgaagtat tagctcaagg gcaaagcgat ttgcttgatt ttttggatga agacgatgac
    26761 gaagctgtat tcgagcttca ctgggataat caacaactag cagaattagt agaagtaaaa
    26821 cagcaaaatg aagcagatac ttatttaccg tacccaagtt attaaggagt gacagatatg
    26881 aaatggaatg aagtaaaagt agaaactgaa agtgaagcag tcgaagcagt tgctaatatt
    26941 ttgatggagg caggggcgag tggtgtcgcg attgaagatt ctttagatgt tgaacatttt
    27001 cagtcggatc aatatggtga gattttagat aaaaatgatt ttacatcgct gaaagaaggc
    27061 gcactagtca tggcttattt tccggaaacg actttcttac cggaaatctt gccttttatc
    27121 aaagatcgta tcacacaatt acctgagttt ggtctagcta ttggaaaaaa taaagtaaca
    27181 gttagtgaag tagaggaaag caactgggca acagcctgga agaaatacta tcatccggtg
    27241 cgtattacgc gaatgctgac ggttgcgcca agctgggaaa actaccagac agaagatcct
    27301 ctagaaaaaa ttatcacact agatcctggt atggcatttg gcacaggcac tcatcccacg
    27361 acttccttga cattgacagc actagaaaca gtcttgcgtg gtggtgaaac attgctagat
    27421 gtgggaactg ggtcgggcgt gctaagtatt gcagcaaaat atcttggtgc aaaagatgtc
    27481 tatgcctttg atttagacga agtagccgtt cgttcggcga aagaaaatat ggatatgaat
    27541 gaaatagcca aagatgtcca tgtatcagct aacgatcttt tgacaggaat cgagattgaa
    27601 agtgatgtaa tcgttgcgaa cattttagca gatatcatct tattgatgat cccagatgct
    27661 tggcgtttac tgaagcaaac aggcacgctg atcgtttcag gaatcattga agagaagaag
    27721 caaatggttc tggatgccat gttagaacaa gggtttatcg tggatcaaat tttccagcaa
    27781 aaagactggt atgcaattat tttgaaaaaa ccagaggtgg aatagatgca gcggtatttt
    27841 ttaaaagaat catatgaatc aaaagactat tttgttttag aaggggaaaa ctatcaccac
    27901 attgtacggg ttatgaggat ggaacccgag gatgaagttt ttttggcatt caatgatcaa
    27961 atagccattg ttgcagaaat cacggagatt actgaagagg ctgtttatct gaaagaaatt
    28021 aaaaaagaag aaaatcaaaa ggaactgcca agttcgatta ccatagcgag tggctatcct
    28081 aaaggggaaa agctggattg gatcgttcag aaatccactg aattaggagc gcaagctttt
    28141 cttggtttcc cggcatcagc ctctgtcgtt aaatgggatg caaaaaaacg tatgaaacga
    28201 caacaacggc ttgaaaagat tgctcaagag gcagcggaac aatcccatcg gcagatcgtg
    28261 ccatcagtag agttattgga aacgaaacaa gatttatttg agcggctaga tgattatgac
    28321 gtggtcttgg ttgcttatga ggaatcagca aagcaaggcg aaaaatcaaa tgcagccaaa
    28381 attcttagtg aaacaaaacc agatagtcga atcttagctg tctttggacc agaaggtgga
    28441 ttatcccgcg atgaagtcga ttattttgta gaacacaaag ggatactttg tggattagga
    28501 ccaagaatcc ttcgtacaga aacagcacct ctttatttat taagtgctgc tagctatcaa
    28561 tgggaattaa tgaatccttc tttcagctaa cgttcttcat taataaaaga gtatcgacag
    28621 aaagaattga aaaaatagtc aacaatttgt ataatagaaa caattattta tacaatggat
    28681 acgaaaaatc gtttgaaaaa gcttttaaaa agcgaacatt tccgtaaaaa aacgtataac
    28741 tgcacggatt ttgggacaat tatcaaacag atatttgtcc cgattcccgt tttaccgaaa
    28801 aaagaaaggg ggatcgattc ctgtgacaaa agaagaaatt ttaacgggac ccggtgtcat
    28861 aaagctcgtt agtcaataca tgggtcccga acatgtagca tttgttgaga aagcatgcga
    28921 atacgcgact gctgcacatg acggacagtt tagaaaatca ggcgaaccct acattattca
    28981 tcctatccaa gtcgcaggta tattagcaga tttaaaaatg gatccccata cagtggctac
    29041 aggcttctta catgatgttg ttgaagatac agaaatcact ttagaagatc tgagagaaga
    29101 atttggcgat gacgttgcta tgttagtgga cggcgtaacc aaattaggga aaataaaata
    29161 taaatcccac gaagagcagc tagcagaaaa ccaccgaaag atgctgctag caatggctca
    29221 agatttacga gttatcatgg tcaaattagc tgatagacta cataacatgc gtacgttgaa
    29281 gcacctgcga gaagataagc aaagaaggat cgctcaggaa actttagaaa tctatgcgcc
    29341 tcttgcacat cgtctaggga tcagccggat caaatgggaa ttagaagata cagcacttcg
    29401 ttatctaaat ccaaaacagt attaccgtat cgtccacttg atgcagacga agagagaaga
    29461 acgtgaaaaa tacgtaagtg gtactgttga agatattcga atagcgacag aagagctggg
    29521 gatttttgca gaaatctatg gacggccaaa acacatttat tcaatctatc gtaaaatgaa
    29581 agatcagaaa aagcagttca acgaaattta tgacttgcta gctattcgag tgatcgtaga
    29641 ttcaatcaag gattgttatg ctgtattagg agcaatccac acaaaatgga aaccaatgcc
    29701 cggcagattt aaggattata ttgccatgcc aaaagctaat atgtaccaat ctttgcatac
    29761 taccgtgatc ggtccggcag gaaatccggt agaaattcaa atccgaacac aggaaatgca
    29821 tgaaatcgct gaattcgggg ttgctgcaca ctgggcctat aaagaaggaa aaaatgaaaa
    29881 agtagaacca gatggtatga cgaaacaatt aagctggttc catgagatac tcgaacttca
    29941 agacgaaagc tatgatgctt ctgaatttat ggaaggcgta aaaggagata tctttagtga
    30001 taaagtctac gtcttcacac caaaaggaga cgttactgag ttaccaaaag gatccggacc
    30061 attggacttt gcatacagta tccatacaga tatcggtaac aaaaccactg gtgcaaaagt
    30121 aaatggcaaa atggtgcagc ttgattacaa attgaaaaac ggagatatca ttgagatcat
    30181 gacttctcca aattcatttg gcccaagtcg cgactggttg aaattagttg ctactagcaa
    30241 agcaagaaat aagatcaaac gtttcttcaa agcccaagat cgagaagaaa atgtgatcaa
    30301 aggccacgaa tccgtggtca aatgtattac agatctagga tttacgccta aagatatttt
    30361 gacgaagaac aaactgcaag aagcactcga tcgttttaat tatcaaacag aagatgatct
    30421 ctatgcagct gtagggtatg gagaagttag ccccttgacg atggccaatc gtctgactga
    30481 aaaagaacgt aaagaacaaa aaatcgagca gcaaaagcaa gaagcagaag aaatcatgaa
    30541 tcagccgaaa aaagaacctg acaaaatgaa agtacgtcat gaaggtggcg ttgtcattca
    30601 aggggtagaa aacttactaa ttcgtatcag tcgctgctgt aatccgattc ctggtgatga
    30661 tatcgttggt tatatcacta aaggcagagg gatatccatt catcgtcgag attgtccgaa
    30721 tgttcagcct gacaaaccaa atgtagcaga acgtttgatt gaagtcgaat gggaagatac
    30781 atcgaataca cgaaaagagt atgatgcaga tttggaaatt tacggctata atcgttcagg
    30841 cttattgaat gatgtacttc aaacagtcaa tgcgctaacg aaaaatctca acagcgttga
    30901 agcacggacg aataaagata aaatggcgac gatccatttg acggttggta tccagaattt
    30961 atcccaccta aagagtatcg tggataaaat caaagcagta cctgatgtct acagtgtacg
    31021 ccggacgaat ggataggagg acagatatga gagcagttgt tcaacgggta agtaaagctg
    31081 ctgtgtcagt cgatcagcaa gaaattggaa aaattggcta cggcctactt gttttattag
    31141 gcattcatga aaccgatacg caaaaagatg tggactatct ggtaaaaaaa attgcacaaa
    31201 tgcgtatttt tgaagatgaa caaggaaaaa tgaatattag tgtagaagat gtgggaggac
    31261 aaattttaag tatctctcaa ttcacactgt ttgcggatac gaaaaaagga aatcgaccaa
    31321 gctttgtgga agccgcacga ccagaaacag ctattccttt atacgaatca ttcaatgaag
    31381 gaatcagaag tcgcggcatc acagtcgcta ccggacaatt cggagcagat atgtcggttt
    31441 ctctcgtcaa tgatggacct gtaacgatca ttattgattc taaaaacaaa taaagtaatc
    31501 tggttttcac tagtaaaaac tatgaaacac gtcgcttaag ttagactgat aaactctaac
    31561 ttaagtggct tttttgtctg caaaaagcgc ttcttttgtc agcagtcgga aaaaaagtta
    31621 agcttaaatt atacaagtta ttttttatag gaggttgctg aagatgaaag aagtaccagt
    31681 agtagatatc aaaatcacag atttaaaaaa attgtatcaa aaaggataca acatgttgga
    31741 ggagttacgc catgaagcgg acgctcccgt cgtcaaagca aagattttca ataaagaagc
    31801 tatcacgatc tacggatcat cagcagcaaa agtattttat gatccaagga atttcaaacg
    31861 aaaaggtgct atgccaaaat tagtattgaa gacacttttt ggtcaaggtg gagtacagac
    31921 tttggatgga gctgctcatc accatcggaa aaatattttt atggatctaa tgacaccgga
    31981 aagaatggaa gattatcatc gtatcttaga taagaatctg acacaagcac ttgaagcaca
    32041 gcatggtcaa tttgaactat ttgatttaag taagatggtt ttctttacat ctatttgcga
    32101 gtgggcaggt atcaatttat cagcgatttc taaagatgag gtcgaaaaat tagcagaata
    32161 tcaaatctct atgatcagcg gaacgtttac ttctccgatt gatcacataa aaggtgtcga
    32221 gaatcgcaaa aaatcagaaa aatgggcgca aggcttgatt gaagaagctc gacaaaatcc
    32281 tgtggctgga aaagaaaatg tggcattata tgcttttgcc aatgccacag atttagatgg
    32341 tcagctttta cctctggaag tggcagctgt tgaattattg aacatcatcc gtccaacggt
    32401 tgctctaact gtgtgggcgg cattgatggg acatgcactt ttcagtagac cagatttgta
    32461 tcagcaatta aaaaatgact tttcgacgtt acaagatccg tttattcaag aaatgagacg
    32521 ctattatcct ttcttcccaa tgctacctgc gatttcgctg aaagaagtag aagtagatgg
    32581 ttatcgtata ccagaaggca gttgggttat tttagattta tatggaacag accatgatga
    32641 acgaacagtt gaagcgccag attcctttat gatcaaacgg tatgtaggga aagcaaaaga
    32701 tatttcctat aaagaagaat atgagatgat tgcacaaggc gggggaaatt ttcgacaaat
    32761 gcatcgctgt gcaggagaat ggatcactct tcatagtcta cgtgtgtttt ccgatcagct
    32821 agtaaataag tttgagttct ctgtacctga acaagattgg acgattccat tcaatcagtt
    32881 tccgacttat ccgaatagcc gagcgctact ttacaagaat tagattagca taaacttatc
    32941 gaaaaaaagg aagatcaaac agtgctgatc ttcctttttt catattacta taaattgata
    33001 aggtcaattg atcaaaatcc acgaaatgct tcatcaatgg tttgataatc ttcattggat
    33061 aaatgcaaat ctaaagcttg gacattgtca gatacttgct ctggacgctt agctccaggt
    33121 ataactacag acactcgtgg attcttcatg taccaagcta aaacaagttg cgcaggagtg
    33181 gcttggtatc ttttagcaat ggggcgaagt acatcgacag cggttaaaat ttctctgaaa
    33241 cgttctcctt ggaaatctgg atttcttttt cgaggatctc cttcaccaaa ttgtttttcc
    33301 tcaccaagtt catattttcc tgtcagcaaa ccggaagcaa gaggaaaata gggaacaaaa
    33361 ctgattttgt ttttttcaag ataagggaat aattcttttt cagcctgccg atggatcaaa
    33421 ctgtatttat cttctactac atcgacatat ccatctgcat tcgcttcttt caattgttct
    33481 aacgtgaagt ttgacacacc gactgctcgg atttttcctg cttcttttaa ttcgtgcaat
    33541 gtagcaactg attcattttt tggtgtgctt tcatccggaa agtggatata aaaaatgtcg
    33601 atgtaatcag tttgcagacg ttttagtgca tcttccaccg attgtttgag aaactcagga
    33661 gaattatcaa atgttcgccc tttatttggt acatgagcag cttttgtagc gatgacgaca
    33721 cgtgagcgat catattcttt ttccttcagt acctcgccaa tcagttcttc ggaacggccg
    33781 tttccgtaag cataagcggt atcgatcaaa ttgatcccgc tattcaaagc tgtacgaact
    33841 acttgttttc ctgtctcatc ttccaaaccg tcaaaaagat tatgtcctcc aacggcattt
    33901 gctcctaatc caagctgttc tgcgtagact tgtgaatgtc cgattcttat ttctgccata
    33961 gctgattcct cctttttgtc tagtctaaaa caaaatacca aaaagctgaa ctaaaacgtt
    34021 tttgtgaggg gaaatagtca gatatctctt gactttgaga ctggttttta gtaggttata
    34081 agtaatatcg aatgtcaatg aaagagcgag taagtttttg aaactttaca agagaggatc
    34141 atcgaggctg aaaatgatct aaagtttcat tagcctaaag acactctgga gatggacgag
    34201 tgaaaagccg tcccgcgtac gagcgttatt cgtaagagga aagagaaatc tttcaaaatt
    34261 aggtggtacc gtgtatgaat ttcattcacc cttgttcatg atgaacaagg gtgatttttt
    34321 tatttacata gctaagaaag ggagagaaaa ctatgagttt ccaaagacca aaaggaacaa
    34381 atgatatttt gccaggcgaa tcagaaaaat ggcagtttgt cgaagaaaca gcacgtttat
    34441 tattcagaga ttatcagtat aatgaaatgc gtacaccgat ttttgaacac tatgaagtga
    34501 tatcccgaag tgtaggggat acaacggata tcgtttcaaa agagatgtat gatttttatg
    34561 acaaagggga acgccatgtg acgcttcgac cagaaggtac agcaccaatc gttcgttctt
    34621 ttgttgagca taaattgttc ggcccagagt atgcgaaacc gttcaaaact ttttatatgg
    34681 gaccaatgtt tcgctacgaa cgccctcaaa aaggacggtt acgtcaattc catcaaatcg
    34741 gagtagaagc atttggatca gaaaaccctg caactgatgt ggagagcatg gtgatggcac
    34801 ttgatttctt caaacagttg gggatcacgc aaatccggct agtcatcaat tcattaggtg
    34861 ataaagaaac gcgtcaagcc tatcgtcaag cattgatcga ctatctttta ccttttgaat
    34921 cagagctgag tgaagactct aaacgccgcc ttcatgaaaa tccattacgc gtattggaca
    34981 gtaaagacaa acgagatcaa aaatttgtgg cagaagctcc atctatctta gattatctaa
    35041 gcgagcctgc tcaaaaacat tttgataccg ttgtttccat gctggatgca ttggctgttc
    35101 cttatgaaat cgacagtaat atggttcgcg gactagatta ttacacacat acgatttttg
    35161 aaatcatgag tgacgcacca aaaatggggg cacaagcaac gatttgcgca ggcggacgtt
    35221 atgacaatct agtggaagaa ctaggtggtc cttcaacacc tggcttcgga tttgcaatgg
    35281 gtatcgagcg attactcttg actatggagg cagaggaagt agttatccct gcatttaatg
    35341 agttggatgc ttacgtggtc gcactcggag acgaaacaaa tatcgaagca ttgaaagtgg
    35401 tccaagcgat tcgaaacttt ggtttttcag ctgaccgcga cttcatgaac cgtaaagcaa
    35461 aagcgcaatt taagacagca gataaagcga atgccaaatt agtattggtg atcggtgaag
    35521 atgagcttgc tagtcaaacg attaatgtca aagcaatggc aagccgaaaa gaaaaaagtt
    35581 ttccattaga agagatatat gaacattttg atgaagtcta tgatgaaatg actttattcg
    35641 agtaggagga aaaaaataaa tggcacaaag aacgatctat tgcggattag tttccgaaca
    35701 acaattagaa caaacagtta cattaaaagg atgggtacaa aaaagacgag accttggggg
    35761 agtaatcttt atcgacctac gtgatcgtga aggaatcgtt caaatcgtct ttaatcctga
    35821 aatcaacaaa gaagcatggg aaatcgcgga taaatgtcgc agtgaatacg tgatcgaagt
    35881 gactggggaa gttagaagac gcggagaaaa tgcagtaaat cctaaaatgg ccactggtga
    35941 attcgaagtg attgcaagtg aaatcgtgat tttgaatcaa gcaaaaaccc cgccatttca
    36001 aattgaagat gatcaagcaa tcagtgacga aatacgtttg aaatatcgtt atttggatct
    36061 gcgtcgtcca aaaatgacta aaaacatcaa attaagaaat gcgacaacaa aagcgattcg
    36121 tcattattta gatgataatg actttctcga tatcgaaaca ccttacctag ggaaatctac
    36181 accagaaggc gcaagagact acctagttcc ttcacgtgtc catgctggac atttctatgc
    36241 gttgccgcaa tctcctcaat tgtttaaaca attgctgatg aatgctggat tcgaccgtta
    36301 ttatcaaatc gttcgttgtt tccgcgatga agatttacgt ggagaccgtc agcctgaatt
    36361 tacgcaagta gatattgaaa caacgttctt aacagcagaa gaaatccaaa cttacacaga
    36421 gggattgatt gcgaaggtaa tgaaagaagt tcgcggtatc gaagtcacat tgccatttcc
    36481 tcgtatgacc tatgatgaag caatggctcg ctacggcagc gacaaaccgg atacacgttt
    36541 tgatatggaa ttgatcgatc taagcgatac agtcaaagaa gtagaattca aagtcttcca
    36601 aatggctctt gaaaacggcg gtgtcgtgaa agcattgaat gctaaaggcg ctgcagatcg
    36661 ctactcacga aaagacatgg atcagttagg tcaatatgta ggacaattcg gagctaaggg
    36721 gcttgcatgg ctgaaagtag aagaagacgg attgaaaggt ccaattgcta aattcatggg
    36781 agaagcaaca gaagcaatca ttaaagcaac agatgcaaaa ccaggtgact tgctgatgtt
    36841 tggcgcagat aaaagtgaaa tcgtcgctgc tgcattaggt gctatccgga cacgtttagg
    36901 aaaagaatta ggactaattg atgaatcgaa attcaatttc ttatgggtaa ctgattggcc
    36961 acaatttgaa ttttcagaag aagaaggtcg atatgtctct gctcaccatc cattcacgat
    37021 gccaaaagaa gaagatatcc cgttattggc tgaagaccct gcaaaagtct atgcagaagc
    37081 ttatgatatc gtcttgaacg gttatgaatt aggcggaggt tctatccgta ttcatactcg
    37141 tgagcttcaa gaaaaagtac tagaaactct aggctttaca aaagaacaaa tggaagaaca
    37201 atttggtttc ttgttaacag ctttagatta cggattccct ccacatggcg gtattgcatt
    37261 aggattggac cgttttgcaa tgttattagc cggagaagat aatattcgtg aagtcattgc
    37321 ctttcctaaa aacggaaaag cagcagatgt gatgagtgat gcaccaagta tggtttcgcc
    37381 tttacaattg ttcgaattga atattgatgt aacgaatata gaagaatagc cgataaaaag
    37441 gtaaaaagga tttgtcgaat acgcttcttt tgacaacctt gatttaattg aaaaacactt
    37501 tataatgaag ccataacata taaagcgggg cttttccatg aaaaaaacat tcgaaaattg
    37561 ggcttatcat gattacacga ccaagctttc ggtatctatc gtttatgcgt tgatcgcatc
    37621 agtcgcattg aatttctttt atcagccagg acatatttat tcaagtggta ttactggtct
    37681 agctcagatt ttgacgactt tgtcaaaatc actgatagga ttcgaagtac cggtatcaac
    37741 aactttgtat ttactgaata tacctttgtt tgtattggcg tggttaaaaa tcgggaagaa
    37801 gtttacaatt ttcactttta tcacagtcac tttgacctcc ctttgtatcc atattttacc
    37861 ggaaacagtc ctatctaccg atccaatcat ctgtgcgttg tttggggggg ctgtgatggg
    37921 actaggtatt gggctagcgt tgagaaacgg cctatcatcg ggcggtctag atatcgtgag
    37981 tatcacgatt cgtaaaaaaa caggaaagtc gattggttct atttcgatct acttcaatgc
    38041 attgatcgtt tttgttgccg gaattctatt tggctggcaa tatatgtttt acagtacgtt
    38101 gtctattttc gtcagtggaa aagtaactga tgtcgtttat actaaacaaa agaagatgca
    38161 ggtaatgatc gtaacgaaac atccagaaaa agtagtaaat gaaatccaaa actgtatgcg
    38221 tagagggatc acgatcatca atgaagcaga aggtgcttat aagcatgata agcagacagt
    38281 attgatgacg atcgttacac gatacgaact gcctctactt agagaagcaa tgaaagcttc
    38341 tgatccacaa gcttttgtaa gtattgcaga aaacgtccag atacttggtc gtttctatga
    38401 ggatggcatt tagatcgagt ttgacaaaaa ttcatgaaat actgctgtag acagacgttc
    38461 gtttgtctgc agtttttttc taccatagta gaatcagcaa agaaaaactg cttttttatt
    38521 tgaaattttc ttaaagttga aaaaaagcat gtttattttt ttaaatttat gggatgatag
    38581 aaaaaaatag tagagaaagg acacgattct acttgctatt ttcacttaat acccgtatat
    38641 tacgaatgaa aaaagtttat gaaaagttgg aatattgaat ggaaaaacaa aaaaggttaa
    38701 aaaacagccg ctacatcact gggtttgatg ggctccgttc attagctgtg gtgggtgtta
    38761 tcttctatca ccttctgcca actagtatga aaggcggcta tttgggcgtt ccgatttttt
    38821 tcgtcgtatc tggttatttg atcacggatc tgctgcggca ggaatgggaa caaaatggaa
    38881 aaatcaatat ttggcaattt tatgtcagaa gaatgaagag actctatccg gcacttgttt
    38941 ttatgcttgt gacagcttcg gcttatatca ctttgtttca gcgagggctg ttaaacaatc
    39001 ttcgaggagt tgtggtcagc agcttactgt atgtcaacaa ctggtggcaa atcaataacg
    39061 gtttatctta tttcgatcga tttgctaatg agtcaccgtt tacgcatatc tggtctttag
    39121 cagtagaagg tcaaaactat ttggtttggc cgatcttgtt tgtattgttg atggtatttg
    39181 ttcgtaaaag aaaatggatc ttttatactg tgttaggtac atccttcctc tccgccatct
    39241 tgatgatgat cttgtataca ccaggaggcg atccaacccg tgtttattac ggtaccgaca
    39301 ctcgtctgtt ttctatatgg ttgggaagtg cgcttgcgtt tgtttggcca agtacgcgtt
    39361 taaagaaaaa catcccgaaa caagcgaaaa atattctgaa tatcgctgga ggaatctcgc
    39421 ttttggcact gattctttgc ttcttctttt tagatgatca ttacaatttc atctattacg
    39481 gaggaatgct cctagtgagt ctgttatgcg tagtgctagt tgcagtgaca gcacaccccg
    39541 gagcaagttt gaatcgatgg ctgacaaatc cagtatttac ttggattggc aaacgaagct
    39601 atggcattta tctgtatcag tttccagtta tgatttttta tgaagcaaag gtgactgata
    39661 ttgccgatca tgttttgttg catactttag tggaaatcac attgattcta atcatcagtg
    39721 aactttctta tcgatatgtg gagcgtccgc tagcacgttt tgattatggt cgtacattcc
    39781 aagtgatgaa aggatggttc agcaaaccga tcatcagtaa gagcaaacca tgggttgttc
    39841 caggcacatt acttgtagca attgcactcg ttggtttcgt tacggcaccg aaaaacgctg
    39901 taacagccga tcaaaaacaa ctgcaggcaa aaatcgctga aagtaaaaaa ttagcagaag
    39961 aaaccagaaa caattcagaa aacaccacta gttcggttga ccaagcgatc cttgataagt
    40021 acgagttgtc caaggcacaa gggaataagg cacaaaaatt agaactaacg gcatttggtg
    40081 attcagttat gttggatgct gcaacggatc tacaagaact ttatccaaaa gtagtggtag
    40141 atggagatgt cggacgacaa ttatatgcca gtgtaccgta cattgaaaaa ttaaaggaac
    40201 aagatttgtt gaaagacact gttctgattg gactaggaac gaatggctca ttctcggaag
    40261 ctcagtttga tagtgtgatg gatgcaatcg gtgatcgaaa agtatactgg gtcaatgtcc
    40321 gagtaccgac gaagcgctgg caaaatgacg tgaatgcgat gcttgaacag atgacgaaaa
    40381 aatacgacaa tatgacgctg attgattggt acagctacag taatgaccat agtgactggt
    40441 tttatgatga tcaagtccat ccaaatcctg aagggatgac acattacatt cacttagtca
    40501 gcgaagcctt gctaggagga agttcaagta cgaacagcag tgagacaaac gtgacagaat
    40561 gatagctaca aaaaacttca ggcaaaaaca tttgtttttg cctgaagttt ttttatattt
    40621 accgtgtttt taacattcat atagattcag gaagcaaaac ggttgagtgt gtctgtaaat
    40681 aacgttcttt ttgttttcgg gtgagttttt tgaatgcagc ttctgctttt gttgcttcgc
    40741 ttcttgaaga aaatacttct gcatggatca tcttaacagg tcttcttttt tgaagacgag
    40801 tatatttagc accagtacca ttgttatgtt cctctaatcg tctagaaaga tcggttgtat
    40861 atccaccata aaatgtctga tctttacaga ataatacgta aaaataatgt tcatttgcca
    40921 tagagcatct cacgaatctc tgcagtgtat tctccatcca tattgtaagt gatcaatggt
    40981 ggagcgactt tgaatccgtc taatttgcct tctttgattc cttcaactaa caaaatattt
    41041 gcctcttttc caggttttgg gtaaacaaat tggatccttt taggtgcaat tcgatagtct
    41101 tccatgacct gaatgatttc taagaaccga tcaggacgat ggaccatagt aaagcgtcca
    41161 tttgttttta gtagcttact tgaaatatcg atcacttctt ctaacgtcgt atgaatctca
    41221 tgacgggcga ttgccaggtg tttattaggg ttcgttttat ttgttggcag ccctttgaaa
    41281 taaggtggat tacagacgac taagtcacaa gaattatgtc gaacgacaga taagctgtct
    41341 ttcagatcga tggtgtggac agtcatttgt tcatttaaat ggttaagcgc gatactacgt
    41401 cttgccatgt ctgctagacg aggttgaagt tcgatagcgt cgatagccgc ctgggttcgt
    41461 ttacttaaaa aaagtccaac tgcgccattt cctgcacaaa ggtcaacaat cttccctttt
    41521 ttaggtacac gggaaaagtg agcaagcagc acagcatcta aagaaaaaga aaacactttg
    41581 gaactttgga taatcttgat atcttctgcg tataattggt cgattcgttc gtcttcgtga
    41641 agcatggatt ccctccaaaa tgaaaaaaat agtcgtctgt attatactat ttttattaaa
    41701 aagaaccaag gtggcttgtc aaacctctat tttttatgca tactatgacc ataagaaggg
    41761 aaaggaagag actatgtttt atcgatttat gcgcggggta gttcgagtac tattagcctt
    41821 agttaatgga aatgcccgct atgagaataa agaagtttta ccgcaagatg agaattacat
    41881 actagttgct ccccatcgta cttggtggga cccattatat ctggcggtag ctgcagcacc
    41941 caaggaattc agctttatgg caaaagaaga actgttcaaa aatccagttc tgcgatttat
    42001 attaactcga tccaatgctt ttccagttaa aagagaaaat ccaggaccaa gcgtcattaa
    42061 aacacctgta aagattttga aatcgacaaa tttgagtttg atcatgtttc ccagtgggac
    42121 aagacatgct acagaactta aaggcggaat ggcattgatt tccagaatgt ctaaagcgaa
    42181 aatcgtgcca gctgtatatc aaggtccgtt gacacttggg gatctgttca agcgtcagag
    42241 agtgacagtg cgtttcggcg aaccaatcga cttatcagat atcaagaaaa cggataaaga
    42301 aggtatggcg gagatcgaac gacgaacaca agcagctttt gacaaattgg acaaagaagt
    42361 gaaccctgat ttcaaatatg aagtcaaaaa aaaataaaat gataagttta tgcgtgggcg
    42421 gtcttttgaa tctactgtcc tgttcagttc agactttttc aatgaaaacg agggagagac
    42481 gtcaagtctg ccctcttttt gcgttccaat atgtaaaata gaagacaggt gtcatgtaat
    42541 ggatcaaagt gttataataa ttagaaggac attttttgag gtgattgaat gcgtttttta
    42601 catacagccg attggcatat cggaaaaaaa ctacaaggtt atgacttgat tgaagaccag
    42661 cgtcatgtac tatcttctat actagatatc gcgaaagaag aaaaagtaga ggcaatcgtg
    42721 attgccggag acttatatga taggagtgtc ccagctgttg atgccgtaga attatacaat
    42781 gaattgatgg tcgattggaa tttaaaggaa aagatcccga tttttgctat atcaggaaat
    42841 cacgatagca gtacgagact ttcagcgggt acgccatggt tcagttattc gaactatttt
    42901 ttgcgaacac agctagcaga agcatttgaa ccagtcgagt ttaaaaacgt acaatttttc
    42961 tttcttcctt actttgaacc aattgcagcg agattatatt ttgaagatga caatatccga
    43021 acgatcaagc aagcggtagc caaggtgata gataaaatgc aggagctatt tgattcagaa
    43081 aaaaaacaag tattgattag ccattttttt gtaatgggaa gtgtaaaatc tgattcggaa
    43141 acgaaaatcg aagtaggcgg gttggattcg atccctggtg aactacttac accgtttgat
    43201 tatgtagcac taggacattt gcataatcaa gctgctttga gacaggataa tgctcgctat
    43261 agcggttccc cattgaaatt ctctttatct gagatcaacc aaaaaaaagg cgtatggatc
    43321 gttgacctga caacaggcct ggagttgaat tttagggaga tcgaacccct gcatgatatt
    43381 gaagaaataa cagcgagttt caaagaattg ctggatcctg ccttttatca gtccgtcaat
    43441 cgggagaatt acatctatat ctatctcact gatagaacga tcatcccgaa tatgatgaac
    43501 cagcttcggc aaatatatcc tcgtattttg gggattgaac gggcaaacgg tcgagcagaa
    43561 caaacaaagc gcaaacgaaa agtactagaa atgaaagatc ctcaaaaatt agcagaagca
    43621 ttttttagtg aaatgacgga agagcagcta actgcaaacc aagaaaaatg gctcggagaa
    43681 acgttgcaag aaatcatgga aatggaatga tcaagaatga aaccgaaaaa attacaactt
    43741 aaaaattttg gtccttttat caatgagacc gtagattttt cacgtttgac agaagcaccg
    43801 ctttttttga tcagcgggaa gacaggagct ggcaaaacga cgatttttga tgggatcacc
    43861 ttcgcacttt ttggtgaaac ttccggaaga cttcgctctg gaaaagagat gcgttctttg
    43921 tttgctacac cagacgaaga aacaagcgtg actttttctt ttgaacatca gcagatgagc
    43981 tatgaaatcg aacgaaaacc agaacaagtt ttagcaaaac gaaaaggaaa cggaacaaaa
    44041 aaacaggcag ccaaagtaac gttgaccatt tttgatggga aaggaaaaga aataaaacaa
    44101 atcacaaaac gtacagaagc agatcagttg atcagagaac tgatgaatct cgatgcgaaa
    44161 cagttttctc aaatcgtttt actaccccaa ggagaattcc gtaatttttt ggtttcatca
    44221 agcagtgata aagagactgt tttacgtcat ttattcggta ctcaattttt ccaacagttc
    44281 aatgagcgat taaaagaaaa agcaaaacaa cagcaacaaa aattggatca tttagagcaa
    44341 gagctgcatc ttctgcaaaa aaggtttgtt ttagtcaaag aagaggaacc agcagcaggg
    44401 tttgaagccg ttttagccca atggacaaac catcaggcaa tactcgaaaa acaaatcacg
    44461 gaagaaaaag aagctctaac ggaacttcag gaaaaacaaa agcaactaga aaattcctac
    44521 tatcgttttg aaagcttgaa aaaaagttat gaagaaaaaa cgatattaat aaacaagcaa
    44581 aaggaattag ctgctcaaaa agacgaaata gaggaaaaga aacgatggat tgggtattat
    44641 gaatttacgc aacgattagt cgaaccaatc cagcgcctac aagaaataaa agttgaaaaa
    44701 atagaactgt cacaaaaaag tagtgagcaa acaaagaaac tagcgatgac tacgcagaat
    44761 tatgaagagt ggcaaaaaaa tgaagcaaac cgcgtacgcc gacaaacaaa aatcaaagaa
    44821 gctgctcaaa aactgcaaaa caaacagatg atggtgccaa ttgtcgaatc cttacgtagt
    44881 aaaaaacaag acgcagaaca attaacaatt tcattggata aagaagaatt gaatgccgag
    44941 aagctgtatc aaaatctcgc tatctatcag gaaagaactc gaattcttca agaaaattta
    45001 ctggatcagg aaaaactgca agaagatcgt cttgcttttg gacagttaaa atatctagat
    45061 gaaaagcagc aagaaataga aaaacaaaaa gcaaaagaca ctgggtcgct cgcacagata
    45121 agagaagaac tcaaaaaagc aactgaagaa ataacaaaaa atagtcagct attgaaagaa
    45181 cagacagaac aagcagaaca gctgaaaagc caatgggcaa agcagcaaat tgctcgtctt
    45241 cagctttttt tgcgtccagg ggagccatgt cttgtttgtg gatcaacgga acaccccata
    45301 caacaattaa aacacgaaca gccaaataag gaagatatct taaaagtaga acaacaactg
    45361 gcagaagctg agtctgctgt agaaagaact cagcaaatca ttgccaaaag tgaagcacat
    45421 gaaaataatc ttcaacaaca attatcagat ttagaacaaa aaatcatggc aacgaatata
    45481 caattggctg aacaagaaga acacttgcaa caggctttga aaaaggcatt tcctgagata
    45541 acagaacaaa aaacaaaaac aactcttgta gtaatcgaac aatctttaga tagacgaaaa
    45601 agcaagatgg ccaatgcaag aatagagctg gaagaaataa gacaacaagc agaaaagctc
    45661 gaacaacaat tagctgtcta caaacagcag cagacagaaa aaaataatca gctccagcaa
    45721 ctgcaaggag aaatcaaaag tttagaacaa caattgcaag atacggatag taactgttta
    45781 gaagaggaga tacgacgact tgaaaaacag ttagaagaag actcgaatca actagctgtt
    45841 gagcagaaag caggagtgca attgttgcaa gatcttcata cgcttcggac acagcttgat
    45901 cttcaacatg aacaaataga aaaaatagat gaaaaaatag aaaaggaaca agaaagaata
    45961 gctaaaaagt tagaaagcca gtcgtatttt tcaacaattg aagaaatctg tgcatttgca
    46021 caacagcaag cacaatataa agaaaaccaa cgtacagtta cttcttatca tgaagagcaa
    46081 ttagtgacag ctgaccggct gcgtcaatta gaaaatatcc agctaactga gacattccca
    46141 gatacagaag agatcagcca acagttagaa gaaataagac gtatgatcga aataaagcaa
    46201 gaaaagatat acggtttaca agaacaaaaa attagtaatc aaaaaatcta cgatgaatgt
    46261 ctttcgattt atcacacaag ccagacgaaa ttagatgagc ttacgcaatt acagcagctt
    46321 tcgcaaacat tcaacggaga aaatcctaag aagactagtt tggaacggta tgtgcttcag
    46381 gtttatcttc aagaagtgtt acaagtagca aatgatcatc tccaacgttt aacgaaaaat
    46441 cgttatcaat ttgagctagc ggataccatt ggcagttatc gagggaaaac cggattagaa
    46501 atcaatatct atgatgatca ggcaggtatg tctcgaagtg cgcataccct gtctggcggt
    46561 gagagtttta ttgcagcact ttctttagca ttagctcttg ccgatgtgat ccagacacaa
    46621 gctggtggtg tagcaatcga tgcattgttt attgatgaag gatttgggtc attagatgaa
    46681 gaagctttgg aaatggcaat ggaagcttta gaaacgatcg aaagtgaagg aagaatgatc
    46741 ggcatcatta gtcatgtaag agaattgaaa gaacgagtat tacaacaaat aagaattgat
    46801 acagaaggta gcggacaaag caaaatacgc tatcaacttg gataaaaagg gagattggta
    46861 actatgaaat ggagtattcc agaaaaagtc attgcacgtg gacgtcaata cttgcaagaa
    46921 ggccgagtgt tatcggttgt ccctgaccaa gaagaaaacg tgtggcatgc agaagtcttg
    46981 ggcagtgaat tgtatatggt tgatttggat ggtacagcaa aagagataga ttactgccaa
    47041 tgtccctatt gggaggaaca taaatattgt aagcatacag ttgctgtgga actttattta
    47101 cggtcgaaaa atctatctcg tatcatgaaa aaagaccagc caatgctaga accggtaaaa
    47161 gcaacaagtg agggagaaat cattgcaaaa ggtttttctc gtctaaaaac acaaaacaat
    47221 cctcaagcgg tgcaaccttt gattattgaa taccaagtag aaacgattga gactaatcag
    47281 tatcacccag agctttctat tttggcactt tacttgcgta tcggttcatt ggcatcaccg
    47341 aaaaaaacat atatcgtaaa aaacatctat gattttttac aagcttacac agaaaaagaa
    47401 acttacactg ttaataaaca gtaccagttt cgtctagata aacgggcgtt tcaaaaagaa
    47461 gatcaaaaaa tactcacgca tcttgcagga agtgctcaga cgcatcaatt attaggtacg
    47521 aacggcttac aagtcaaagg aaaactagat aaaagatatc tgcttctccc agtagaacaa
    47581 gcccgcttcc tgttagagca gatgaatcag actacacgtt tgcatctgca gataaatgaa
    47641 caaaacgatc aaggaataca attttccgat caaaacaaac ctttatcatt caaagtggaa
    47701 aaatttggag aagattattt gctgcaagca ttgaatgatt ttgatttttt ctttatgcat
    47761 tatcagtggg gagttatcca agggacattt tattctctga cgaaatatca acaaacggtt
    47821 tatcaaactt ggaaacaatt gattcgccga ttggagaaac cagaagtagt gttcaagaaa
    47881 aaagaattac cgtctttgtt taaagagatt atccctctgc tgtcagagat cggcgacgta
    47941 gctattgccc ctgcagtaga agaggaagta gcagatatcc ccgtcgaatt tattttcttt
    48001 ttccgaaaag caaaaggaaa aatccaagct cgagtcgatt tcgtctatgg tgaagtcatc
    48061 tattcgactg acccgaagca tgaggtctcg acagaacagg cttttcaggt gttacgtgac
    48121 acagtagaag agaaacgagt acttgatcta ttcagaatgt atcgctacca gaaaaatgaa
    48181 actggttatg aacgtccatt gccaagcggg gaagaactat atgccttttt ccgcacagaa
    48241 ctatctgtct tccgtcagtt tggtgaagtc agacttggaa gaaaattgcg agagttgtat
    48301 ttagatgcgc ataaacatca accgaaaatc gaagtaaccg agaccggttc atggctcgat
    48361 attcgattcg atgtgacagg gatagaggag caagaaatcg atcatgtcct gcaaagcttg
    48421 ctgagaaatg atgcttttta tacattagaa aatggtcagg ttctttcttt tgattcagaa
    48481 gaatttcaac aaacaagcca agtgttgcaa caattacggg aatcgatacg tacagaagaa
    48541 ggaacgatcc atgtacctaa aaatcaagga ttgatcattc aaaatcaatt ggaaaaaagc
    48601 aatgctactt ttagtgaatc gttccaaacg atggtacaag atctcattca tccagaaaga
    48661 taccaggcac agcttcccaa aggattgaat gcaacgatga gagattatca aaagcaaggt
    48721 tttcgatggt tgaagatgtt aggtcattat cagtttggcg gtattttagc cgatgaaatg
    48781 ggacttggga aaacactaca gacaattgcc tttttacttt cggaaaaaga agaacggaaa
    48841 agcttctctg ctttgatcgt agcgcctgcg agcttaattt acaactggca ggcagaggtg
    48901 agaaaattcg ccccttcttt aagtatccaa gtaatcaatg ggaataaaaa agaacgtgaa
    48961 gaattactgg ctaaagacac agatatccga gtgacttctt atgccagctt aagacaggat
    49021 ctagctaatt atcagtcgca aaaaattgat tatttgattc ttgatgaggc acaaatggtc
    49081 aaaaatagca gtacgaaaac tgcacaagca ttgagagaat tagctgtacc tcagcggttt
    49141 gcactgagtg ggacaccaat cgagaataat ttagaagaat tgtggtcatt gtttgcaacg
    49201 attatgccag gatttttccc gactaaaaca aaattcaggg aacttgccgc agaagaaatt
    49261 gcgcaaatga ttcgaccgtt tattttacgg cgagataaac aaactgtgtt gaaagacttg
    49321 ccggaaaaaa cagagatgaa tctatattct gcacttacag aagaacaaaa aactgtttat
    49381 ttagcttact tgcgtcaaat gcgtgaagaa atcacatcaa tggattctga agcattcaag
    49441 aaaaaccgga tcgggatact tgctggtctg acaagactgc gacaaatttg ctgcgatcct
    49501 cgtctgttta ttgaagatta ccaaggcggt tccggcaagc tcgaacaagt aaaagatcta
    49561 ctggtagctg ccaaagagaa caaacgaaga gttttgctgt tttctcaatt tacagggatg
    49621 ctgactatct tgcaagaaga gttagccgag ctagggatct cgacatttta tttaagaggc
    49681 agtacgaaac cacaagatcg gttaagtatg gtagacgcat ttaatgctgg cgaaaaagat
    49741 gtttttctga tttccttaaa ggctggggga actggattga atctcacggg agcagatacg
    49801 gtcatcttgt atgacctttg gtggaatcct gccattgaag aacaagctgc tggacgtgca
    49861 catcggatcg gacaaaaaaa tgtggtagaa gtgtggcgta tgatctctga aggaacgatt
    49921 gaggaacgca tggattcact ccagcaagaa aaacgcgaac tgttccaaaa agtgatccaa
    49981 ggtaatgaag aacagttgac gaaactgact gaagaagaca tccgcatgat tttaagtgta
    50041 ggagaaatcg aggagtaaga gactcgaaaa agcgttcagt aatgggtaag aaagcctttc
    50101 taaagaagcg attgtttatt tgatttaaaa aatagcaagg aggctgggat tccattttat
    50161 cccagcttcc ttgctatcat ttttttattt ctatttttaa aggttataca aagattgtct
    50221 tcattgtctc taacagctga tcaaaagaag aaatctcgat atcgcaaatt ggagtctgtt
    50281 caattttgcg ttctcgatgg ttgaaccaaa gcgcttgcca gttagcactt tttgcaccta
    50341 gaacatcatt gtcgtaatta tcgccaacgt ataaggtctc ttctggaagc atatgaaatt
    50401 ctttctcagc cagctgaaaa atctcttttt ctggtttttg gaaacctgtt gcttgagaaa
    50461 tgagcatatt gcgggaagga atccaattgt tcaattgaag ttgcttcagt ttttttgtct
    50521 gatggtcagt cggcccattt gtgataattc ctaatgggac attcttttcc ttcaagaaat
    50581 cgagggtttt tttgacctcc tcatgcaagc agatattatc aagttcttcc tcgtaaattt
    50641 tttgaaataa gaggccatct tcttccgtaa tatgagggta gtctaaatct tttagtgatt
    50701 ggctgatccg atgtgctcgc atatattcta gtgtccaatc tcctgccatt acttttggga
    50761 agttttcatc actatgatgg cggaaccgga tatatagtgg atgcatatct gcatctgaaa
    50821 caagcggaac gactcttttg accgcgtttc gaaatggctg ctgctgatca tacatcgtat
    50881 catcaacatc aaacacaact gatttaatca aaattttaca cgcctttcgg ttttcattga
    50941 ttttttcaca aatccttgac tattgtaaca gaaaaaattg tctaaaaaag ctgataaata
    51001 aaggtttttc ttactatatt tctatttcct tgacagaata aggtttttat atttcagttt
    51061 ttttcactaa aaatcgtgaa acacgtcaat aagtttgatg aacaaat
//
LOCUS       DACDIG010000011        49055 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.011, whole genome shotgun sequence.
ACCESSION   DACDIG010000011 DACDIG010000000
VERSION     DACDIG010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.011"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(526..1233)
                     /locus_tag="IV247_000716"
     CDS             complement(526..1233)
                     /locus_tag="IV247_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8776807.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENG"
     gene            complement(1298..2134)
                     /locus_tag="IV247_000717"
     CDS             complement(1298..2134)
                     /locus_tag="IV247_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8776808.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(2152..3561)
                     /locus_tag="IV247_000718"
     CDS             complement(2152..3561)
                     /locus_tag="IV247_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8776809.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3575..5398)
                     /locus_tag="IV247_000719"
     CDS             complement(3575..5398)
                     /locus_tag="IV247_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8776810.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(6241..6567)
                     /locus_tag="IV247_000720"
     CDS             complement(6241..6567)
                     /locus_tag="IV247_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8776811.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6613..6975)
                     /locus_tag="IV247_000721"
     CDS             complement(6613..6975)
                     /locus_tag="IV247_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAP8776812.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(7012..7227)
                     /locus_tag="IV247_000722"
     CDS             complement(7012..7227)
                     /locus_tag="IV247_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8776813.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            7354..7689
                     /locus_tag="IV247_000723"
     CDS             7354..7689
                     /locus_tag="IV247_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8776814.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(7765..9762)
                     /locus_tag="IV247_000724"
     CDS             complement(7765..9762)
                     /locus_tag="IV247_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8776815.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(9860..10135)
                     /locus_tag="IV247_000725"
     CDS             complement(9860..10135)
                     /locus_tag="IV247_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8776816.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            10367..11101
                     /locus_tag="IV247_000726"
     CDS             10367..11101
                     /locus_tag="IV247_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8776817.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            11140..12282
                     /locus_tag="IV247_000727"
     CDS             11140..12282
                     /locus_tag="IV247_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8776818.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(12377..13768)
                     /gene="sufB"
                     /locus_tag="IV247_000728"
     CDS             complement(12377..13768)
                     /gene="sufB"
                     /locus_tag="IV247_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAP8776819.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKAVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(13773..14243)
                     /locus_tag="IV247_000729"
     CDS             complement(13773..14243)
                     /locus_tag="IV247_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAP8776820.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(14230..15465)
                     /locus_tag="IV247_000730"
     CDS             complement(14230..15465)
                     /locus_tag="IV247_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP8776821.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(15462..16748)
                     /gene="sufD"
                     /locus_tag="IV247_000731"
     CDS             complement(15462..16748)
                     /gene="sufD"
                     /locus_tag="IV247_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAP8776822.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(16761..17534)
                     /gene="sufC"
                     /locus_tag="IV247_000732"
     CDS             complement(16761..17534)
                     /gene="sufC"
                     /locus_tag="IV247_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAP8776823.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(17731..18579)
                     /locus_tag="IV247_000733"
     CDS             complement(17731..18579)
                     /locus_tag="IV247_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8776824.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(18603..19289)
                     /locus_tag="IV247_000734"
     CDS             complement(18603..19289)
                     /locus_tag="IV247_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8776825.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(19282..20316)
                     /locus_tag="IV247_000735"
     CDS             complement(19282..20316)
                     /locus_tag="IV247_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8776826.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            complement(20711..21049)
                     /locus_tag="IV247_000736"
     CDS             complement(20711..21049)
                     /locus_tag="IV247_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAP8776827.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(21051..21407)
                     /locus_tag="IV247_000737"
     CDS             complement(21051..21407)
                     /locus_tag="IV247_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAP8776828.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(21413..22600)
                     /locus_tag="IV247_000738"
     CDS             complement(21413..22600)
                     /locus_tag="IV247_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP8776829.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(22791..23636)
                     /locus_tag="IV247_000739"
     CDS             complement(22791..23636)
                     /locus_tag="IV247_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8776830.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            23870..24835
                     /locus_tag="IV247_000740"
     CDS             23870..24835
                     /locus_tag="IV247_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAP8776831.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            25212..26255
                     /locus_tag="IV247_000741"
     CDS             25212..26255
                     /locus_tag="IV247_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAP8776832.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(26317..26781)
                     /locus_tag="IV247_000742"
     CDS             complement(26317..26781)
                     /locus_tag="IV247_000742"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAP8776833.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            27027..27974
                     /locus_tag="IV247_000743"
     CDS             27027..27974
                     /locus_tag="IV247_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8776834.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(28185..30257)
                     /locus_tag="IV247_000744"
     CDS             complement(28185..30257)
                     /locus_tag="IV247_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP8776835.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(30190..30567)
                     /locus_tag="IV247_000745"
     CDS             complement(30190..30567)
                     /locus_tag="IV247_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAP8776836.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(30696..31256)
                     /locus_tag="IV247_000746"
     CDS             complement(30696..31256)
                     /locus_tag="IV247_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8776837.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            31406..32698
                     /locus_tag="IV247_000747"
     CDS             31406..32698
                     /locus_tag="IV247_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP8776838.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(32758..33876)
                     /locus_tag="IV247_000748"
     CDS             complement(32758..33876)
                     /locus_tag="IV247_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAP8776839.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(33890..35311)
                     /gene="arcD"
                     /locus_tag="IV247_000749"
     CDS             complement(33890..35311)
                     /gene="arcD"
                     /locus_tag="IV247_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAP8776840.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(35557..36678)
                     /gene="mnmA"
                     /locus_tag="IV247_000750"
     CDS             complement(35557..36678)
                     /gene="mnmA"
                     /locus_tag="IV247_000750"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAP8776841.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            37082..37681
                     /locus_tag="IV247_000751"
     CDS             37082..37681
                     /locus_tag="IV247_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAP8776842.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(37819..39105)
                     /gene="efmA"
                     /locus_tag="IV247_000752"
     CDS             complement(37819..39105)
                     /gene="efmA"
                     /locus_tag="IV247_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAP8776843.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(39376..39720)
                     /locus_tag="IV247_000753"
     CDS             complement(39376..39720)
                     /locus_tag="IV247_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAP8776844.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(39857..40993)
                     /locus_tag="IV247_000754"
     CDS             complement(39857..40993)
                     /locus_tag="IV247_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP8776845.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(41182..42156)
                     /locus_tag="IV247_000755"
     CDS             complement(41182..42156)
                     /locus_tag="IV247_000755"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP8776846.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(42236..43111)
                     /locus_tag="IV247_000756"
     CDS             complement(42236..43111)
                     /locus_tag="IV247_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAP8776847.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            43241..45148
                     /locus_tag="IV247_000757"
     CDS             43241..45148
                     /locus_tag="IV247_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAP8776848.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(45689..48967)
                     /locus_tag="IV247_000758"
     CDS             complement(45689..48967)
                     /locus_tag="IV247_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAP8776849.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
BASE COUNT        14082 a        10314 c         8656 g        16003 t
ORIGIN      
        1 cccttttata aaaagcaaaa aaaaaaagaa agaaacttat tcttgctaat aaactacgcg
       61 aaaggagtct aacgttgttt gatataactt agactgatcg ttaacttatt ttttatattt
      121 ctacatagtt atctttagaa ccatttcaga tacatatgaa acaattacat atgatttttt
      181 cataaaaaaa cagtactgtc tagtagtata aaatatacaa atttcttaaa aattttttca
      241 ctaaaatcac tacctgaaac agtctaaaac acttatatat caacgtttaa tgaaactatc
      301 tccgtttcat ttcagataca aataaatata gtcgggctcc aaatccgaaa aaaaataccc
      361 ccagtagctt atcctaagct actggagatg tataagtacg gtcatataac aacggggtag
      421 ggtaaataaa tgaaaaagat ggaagaatca ttattcaata acttttttaa atacttcgtt
      481 gtaatcagtt acctgtccgt tcttaaatac cacagaataa tctttttacc cattttcaag
      541 taaccgaagt ggaatgtctt taccaacttg ctctgtcact gcattactga tacctgttag
      601 attagctaat aaatctgata aagattgagc atctgtagaa atctctgtat cagtatttat
      661 cgtgattcct ttatcttcat ttacaagctt ccaagtaggt ggtatagctg tgtatgtttc
      721 gatgaatgaa tcaattcttt catctatcgt cttcccagta tctacttctg tctcattact
      781 cttagatgaa tcgattcttt catccatcgt ctgctccgta actgcttctg tctcattatt
      841 ctcaccggaa actctcttca cacccaaact tatatattgt ccatctttag tgtgcatagt
      901 taaaattccc acgcttttac taataaattc actataacca gttctttcaa tagagacttc
      961 aatgtatttc acactatcat actcaaaaga aaaatagaca tgtaaaagca ttttgttgtt
     1021 tacatctaca taataatcct ttgactctac ttgagacctt cctatacgga atacagcttt
     1081 actgtctgat atctctatac cagcatcctt attattaatc tgccaatctc caagtagctc
     1141 ttctggcaag ctagtagttt cttgtgaaca ctttaccact tcactggtag aggtcgaaac
     1201 attcacctcc gcactgttag aagaattttc caaggaatca ggagccttag cacaaccaac
     1261 aagtctttat atattgtgca gccgatattt tcttcattca gtacaccctt gaaatatgca
     1321 gtgttagata aagcagttcg tcctgatttg ctgaggcacc catctcttct tgaacgtact
     1381 ccttgatttt caaggccgcc cgatactctt tcggaaaacg ctgcttcact gaagccagca
     1441 aaaaatcatc cttctccggc aactcttcct tcttaatcat tcgggcaata aaaaactgta
     1501 aatgcgtcac aaatcttaag taaagcatcg aatctttatc aacctttttt tcgggaaaca
     1561 gatatcctag catagccaag atatccttta gtgctcttac cgagagcatg gtttcttcca
     1621 tatttgcttg ttcactttgg gcatttacaa tgtgaaacgc aatgtttcca gcctcctctt
     1681 ctggcaactg aacattcatt tttttgttga tcaattggat agcatagcag ccgatctcaa
     1741 attcttttgg gtaatacttt tgaatttcaa tcagcaggcg attttgaaaa acaatgcctt
     1801 tttgactgcg ttcaatggca tgcttcaagt gatcaaccaa ggtaaaaaat agacttttat
     1861 tcagcttaaa gtcaaacttg gcgttggcat aatcgataat tcgcttgacg caaataatta
     1921 gatcatccgg agcagcctca aacagtttaa tgtaatcctt ttttagagca tcagaaagtg
     1981 aatctaattt aaatactttt tggattttgt cagccggaac caaatcgcct ttctttagtt
     2041 gaaatccaat ccctttcccc atcaaaatta cttctttgcc ttgcttatct aagcaaagaa
     2101 ttaaactgtt gtttaacgtt ttaattactc tcattagatt caccgttctc tctaagctaa
     2161 gccatttctt gcgattactt tttgatacca aaagaagctt tcttttttta ttcgttttaa
     2221 ttctttaaga ttctcatcgg ttcggtcaac atatataaaa ccataacgct tattgaagcc
     2281 ttgatgggta ctaacaacat caatcgctga ccaaggtaaa taaccaaaaa catctacacc
     2341 atctgttaaa gctgcttgaa tagccagaat atgtttggct agataatcaa ttcgatcctg
     2401 gtcattaatt cgtctatctt cctcaactat gtcgggtgcc ccatagccat tttcagtaat
     2461 taaaataggt aaatcatagc gatcgtacaa tttcctcaat gttaatcgta gtccagtagg
     2521 gtcaactacc cagccatatg gcgttttatc tacataagga ttttcagctg cccggtagac
     2581 accttcttct cccaacatga tctgttgatc ccccgctcta gcggaaacat ctgatccgtc
     2641 atttttgctg gcagcgatgg tggcagttga ataataatta atcgcaataa aatcgggttt
     2701 accggaggat aaaattgcct gatcctctgc ctcaatttta ggatataatc cacgatcatt
     2761 caaatagttt tcaaacaaat aattgtattt tccccgaact gctacatcta agaaagacca
     2821 gcctctcaat gtttcccaat tatgggctgc aattgcatct tctggactgg aggtagcttg
     2881 atacatagcc gttaaattga ttgctggacc gatttttcct ttaagattca actgatgata
     2941 caagtgaatt actctagctt gagctaataa aacatggtga ttaatttgaa agagttcttt
     3001 ttttgaaggc agctctccac ttggtggcaa accaatcgct ccaggatgaa ggatcatcgt
     3061 gttttgctca ttaattgtca gccaatagtt caccttgtca ccatactcag taagcaatac
     3121 ctgtacatag tccacaaaat aatcgatagt tgctcgattt aaccaaccgc cctgctcctc
     3181 tagaaaataa ggtaaatcga aatggtacat ggtgacaatc ggttcgattc cctgattcag
     3241 gcattcatca attacttgat gataaaaaga aattcccttt tgattggttt tattattttc
     3301 aatatccata attcgcgtcc aagcgatgga aaagcgaaaa gcttttaagc ctaattcttt
     3361 aaacagctgt atatcttctt tatagtgatg gtaaaaatcc gaggcaactt caaaattagc
     3421 taccccttct gtatgctgat acttgtcaat aatagacggt tgtttcccat cctcctgata
     3481 ggcaccctct gcctggtagg cactaatgga ggcaccccat aaaaacttct ctggaaatgc
     3541 tgaaagctgt ttgtattgca caaaaatcct ctccttaact aatttgcatc aatggttcgt
     3601 gttcctttaa gcttccgctt gcttcactaa ttcctgcgta attttcactg ttggtcacta
     3661 ccacaatcac tgtatcatcg tagccagcgt ttctaatggc agcgaaatcg gcgttgatta
     3721 ataattgtcc tgctgacacc tgatctcctg cttttacaaa tacttcaaag ggttcccctt
     3781 ttaaattcac ggtgtcgatc cccacatgga tcaataattc aataccttct ttagaacgta
     3841 gaccaatcgc atgtttagat tctaaaatag taatgacttc tccctcaaca ggtgaataaa
     3901 tgctgttttc ttctggatga atggctacgc cctttcccaa tagtccctta gaaaagacag
     3961 catcgttcac ttcagaaata ggacacacgg ttccttttac cggattttca acaatgactg
     4021 cttcggctat tgccgtttgc tctgattcat cagaagtatc gtcaaatcca attagataag
     4081 caatgataaa ggttgcaaca aaggcgatta cacacgtaat aattgcgtga acaatattca
     4141 ttggattttc tccaataaag gctggtaagg ctgctaagcc aggggaaaca aaggcatatc
     4201 tgactaaacc gctcaagcca gcatacaaac cagcaattgc accaccaatc atggaagcaa
     4261 ctaatggttt tttcagcttc aaggttactc catagagtga aggctcagta attcccatta
     4321 aagcagtgat cccagtggac acagctagct gcttcaactc tttgttttta gttttcgcgg
     4381 ctacagcaaa ggtagcacct gattgagcaa tattagaagc cagcattcct ggcccattga
     4441 tcatttcaga acccgtactg ctcaattgca tagtagcgat tggcgtcatt gcccaagctg
     4501 tacccgtaac caccaaaaat ggctgcaaac cacccatcaa aaatggaatt aaccaagctg
     4561 ctttgccgtt aatccattca gcccccattg ctataagatc gttcagcaac gttccaaacg
     4621 gtccaatgac aaccaacgct aaaggcccca taattaaaaa taccagcatc ggcttagtaa
     4681 agaatttaat aaacgaaggc gaaattcgat ccgcaaaggg ctctacatag gacatgatcc
     4741 aaacagtaaa gataatcggt aaaactgatc ccgcataatc tgcagctaat accggaattc
     4801 ctagaaaaga aacggcatcg ccagtagaaa aaagctgtga aatatttgga tgaagcaaaa
     4861 tgccagcaac agtaatcgcc aaaattgggt tgacatcaaa ctttatggct gccccatagg
     4921 ctaatagtac tggcataaaa taaaaggctg catcagcaat agtattcaaa atgatgtaag
     4981 tttgactact gtctgatagt acaccagtca aagtcaagac ggccaaaatg gcttttacca
     5041 tcccggcacc tgttatagct ggaatcatcg gtgtaaaggt ggtagaaatg acactaatca
     5101 atcttgaaat aatactttct tgattgtctg aagaggggcc ctttttaatt gaagttccca
     5161 cttccttttg aatttccttg aaggctgttt ggacttcatt gccaatcacc acttggtatt
     5221 gtccaccgct atccaccaca ctaattacac caagtgtttt ttctaaggct ttcgtatcgg
     5281 ctttcctttt atctttgagt tcaaaacgca gtctggtggc acaatgggtt aagctgacca
     5341 cattttcttt tcccccaacc tgttgcaata catttcttcc tacttctctg taatccatta
     5401 ttctctctcc ttaaagcagt aaaaaagacc aagtcaaacc acgccccaat caacattgaa
     5461 gtgttagttt gtcttggtct tgcctgcata atcagtaaca tgccaataac gttattttgc
     5521 tgtaatccaa taatagcaca ttatgatagc gcaatcaaca gggaatttaa ttttttatct
     5581 tttcgtttga ccgtataggg taatataaca gttagatcac atcaactagg gtatatctgc
     5641 gtatacttac tgctaaaata agtatatcta atattaacta taccagtaaa accattccta
     5701 cataaccagc gggaaattcc caaaatcgtg aaagcatcaa aaatgcatat aacggatcac
     5761 acaacttcaa caattctgct cttgggaaaa attgatgaat ttggtgagta aaggatggaa
     5821 taaacagcat tccaacaata taggcaacaa tagtgatact tgtttttttg gtaaaaatca
     5881 gtaagcaata gccaatttca atcattccga gatataaagg tatcgataga atgatttggg
     5941 ctagtattct taaaaattaa ctagcaaatg tttaaactga tttatttcta atctacatgt
     6001 agtaagtatt tcaattccac caattatcaa taaaaatacc aaacatatcc ataataattt
     6061 tttctttttc atttctttta tattgttata acagtgaata acataaaaat tcccgacaat
     6121 cattgtaata tttttttgtc acctgtatta gtatttaatt tataggaaac tataaaaact
     6181 ccctagtgaa tttacacctt tgggagttcc tgtttggacg gtatgcataa tacatttaaa
     6241 tcaatttaac attaacacct cagcatatgg tatctccaca tatatacggt ctttctttgg
     6301 atagtactca atatttatgt tgatagcact tccattgtct aatatagctt tagcccggta
     6361 tgtgttatct ccattttcat gagtaatcgt cacatcatca catgatcgat ttaagttgta
     6421 ctgttcttgt aaaatctgtg ttaccaatgg ttttgatgac ttctctaatc cagatgtacc
     6481 tgtcattttt gaaaaaataa tacctataat agccaagaca acaataccta taatgacaaa
     6541 cttcttgttc atatttactt tattcaactt tttatctcct atctccaaaa taatttgttc
     6601 ttatccctct tatcagtaca ttgacatccc taaaagtaca ccaaagattg ctccagctac
     6661 ccctgctatc atcaaaatcg tacccatttt ctcatctttt tctcgatata agtagcccat
     6721 aataacagca acagcaccaa atacaacagg aataaatgct aacgatacta ccatagaaat
     6781 ccaaccaata atcaaataca gcttccctgt ctcttgtcca tgtacgattt gttccttcat
     6841 ttcattaatt gtctgctcag tacttttctt tactgtgtct gttccggttg tctcatctgt
     6901 ttcttgtagt gctccacaaa actggcaata cttactttca tctgttattt ctttgccaca
     6961 ttttctacaa tacatgtctt ctcctcctaa aatcattcaa ttgcttatta gttaactgtt
     7021 tactttatta aatacatcag aaaacagctc attcattggt acacttaata tctcagaaag
     7081 catcttcatt tgatagggtt taggaatcgt tctccctgtt tgccacgaac ttacacattg
     7141 ctgtgtaaca ttcatttttt ttgctaactc cgtttgagaa agtccttttt cttttctgta
     7201 tcggctgaga ttacttactt ttttcatgat tctcccctcc tgttttatta tatacaacaa
     7261 tatgatgtat ttcaactcat aaaatataaa aaacttcaaa taccattaca aatacaacaa
     7321 cttgttgtac tatactgaga tgggggtgta ttaatgaaac agaaacaata tgcaaaggtc
     7381 attagagaaa agcgaaaagc tctcgggtgg acacaaaaag aattagctga aaaaatcttc
     7441 tctacacaac aggcagtagc aagatgggaa aattctgtaa cagagcctaa cctcgatagc
     7501 ttaacagctc tttctagagc cctaggaacc cctgtaagcc attttctaga caatgttgtc
     7561 gtagattatg aggaagagtt tttggctctc tatcgttctc ttagcactga agatgcagtt
     7621 cgaacaattg actatatgaa gctattaaaa aggcaagaaa atgaacgcaa tcagctattg
     7681 aaagattgaa aaataaataa cccaccgaac tttaacggtg ggtgtattga gatgaaataa
     7741 gattaagagc tatggataac ctgtctagtt taatacaatt gggtcttttc tcttccttac
     7801 tctccgtcca tcatctcttg ttacacttgt aaattgatac agaggaacta tcccagctcc
     7861 tgataatatc aattctttta ttgtttcatc tatccctgcc cctttcgcta aatatattaa
     7921 aaaactacta cttgctttca cattgtatag tttcttaata tccttagaag ctcgttcaag
     7981 agtgaatcct tgtttaacaa agtagcctct gttttttatt aaagcatttt tcacgctgtt
     8041 atccccaaca ttacgtaggc gttcacaaac agtgttaata agttctttct ctgtcattag
     8101 atatgtccct tgccacgata agccctgtaa aacttcatgt gcttcatcta aaatcaattg
     8161 tttttccttg ctgagttctg atcgggaatt ttgaggaaat actcttttag cctctttttc
     8221 gttaaaacat atcttaaccc attcataaga aaaatattga tatgtaaaat gaattttttc
     8281 cagaagtgga aggatatctt cattcaattt ttgaatcaca gaagttgcat taaaaacgtc
     8341 taatcgatag acattgcttc tcttatgatt aaagtttcct aacgctttct cttcctttaa
     8401 cctatttagg tattcgagca tcttcaaagg aacatcttca tcatctaatt tttttatcaa
     8461 tcctataaaa gtcataagtg atagtaactt attacattta tctatgtcat aagttttacc
     8521 atagtaagaa ctcatttttt tagctaattg ttcccccgac aaaatacaag tcacactttc
     8581 cttactatca tcaaaaaaat taacatcaga aagcaagaca tcatagacac gtcgcatttc
     8641 ctctagataa ttccttattg tcctgaaaag tttgtctttc attgattcaa gagtgtcaaa
     8701 aagtactttt gattcctctt tcaattttcg aaaatgtgta ggatacaagt ctaggttctc
     8761 taatagccaa ttaaatgttt ctacccgact gtctttcttt aaaattagca tcacaactcc
     8821 aataaaattt tcactgatat cacaattact ggaatgacaa taaaaaatgg aatgcccagt
     8881 tttactggta aaaatactag cagagggatt atgatcgttg tgaaacagac atcttaagtt
     8941 tttacttgac ttaagtaatt tattcatatc aatggttaac aatctttcat aaatctcatt
     9001 aaaagacatg tcggaggaat caaacaggca gtctttaaac cactgtttca tttcttcttt
     9061 gtcgtcattt tttatcaact taacaaaatc ggtttcattg actagttcat actctctccc
     9121 tgaatcactt tgacaatttt tagaacatgt catggtaaaa tcaatcggaa gctcagtaat
     9181 atctaacatg ttgtcttcat taattattat cactgcatct gacttatatc ctccaaaaaa
     9241 cattctattc gaagcattta cattgtcatc acacccgggg atgttctttt tcaagtattc
     9301 gtaaacagca accattttac catagtcagt aattgcttct tttaatagaa agacaatctt
     9361 gtatttttct agattttcag agtaccgaat acttttttgt atcgcaaacg cagaattatg
     9421 aactaaattg atagataaaa ccttttctat gcttaagtat ctactatcat ctttactcaa
     9481 atctactacc ttccccttct catctttaac agtgttatcg acatccagaa taattactct
     9541 ggtttccaca aaactctcct tgtcattgcc tttattcttt gtcttgcttg gaataaatga
     9601 ttgtgatgaa tttagtatct ctacaaactc atctcttgta acttctctat agttatctaa
     9661 acacctatct cttacatcag ttttttcttt ttttgtaact ggcttctcta ataatttttc
     9721 ttgatctaca taaattggaa tattaatcat tgatgtttcc atttgtgttt cctccttata
     9781 aaattctgtt aaagactaaa acatacagat aataaaaaca aactctttat tactatatgt
     9841 tttagtctta tttgtttact caacctttat accgttccat gaaactatca atatcagatt
     9901 ttttaaatct aatgaccgta tcaattttta taacaggtaa cccgtattga gaaacatatt
     9961 ttctaatcgt tcctactgat acacctagat attcacttgc ctgctgatag tccatatact
    10021 ctagagtgac tccagaaata atgtcttcta gcataggctt tagcactgta attatctctt
    10081 tcacttgttg ttcaacttgt atagaaacaa tgctatcaat gatttgtaga gacatatttc
    10141 cccctccttt cgtgattgct tgaacaaatt ttctaagaat tatcttcgta ttttttgctc
    10201 aaaataaaag tattttgtta ttaaccgatt aatcaaacaa aaaatgatta ccagttcatc
    10261 gacatacaca taatacctcg ataaaaaagg ggtgtcaaca gctttgtaaa atttatttac
    10321 aacacccctt ttttgaggta tactaatttt aggaggtgtg gtcaatatga taaaacgtcc
    10381 accaataaat tatttagaac gaaaaaagat actcggaaca aaaataaaag ctataagaaa
    10441 gagtaaaaaa ttaacacaac cagcatttgg tcttatgatt aacaacggtc aattaattga
    10501 caaaaaaact atctacgaat gggagaaggg aacctattta ccaataccag agcgattatc
    10561 ccgaattgct gatttgggaa atatgagtat agaggagtta gtttgtggca atgtagaaga
    10621 atatattttg ggaattattc tttatagaga tagcattgta ctagatggta taacatttcc
    10681 agataaaaac ctgttccagc atcttcgtca acagttccca ccagtacact ctaatttaga
    10741 tacatggctg gatagatatt ccaaattaga accagagatg caagaattta ttgctaacaa
    10801 gacttgtaat aaagttaaaa atgaaaaaat tagtcttttc aatatcttaa aaatagaaga
    10861 attattcatc aatgctattg tagaagaatt tgacaataac attcttttcc tcactagcag
    10921 tatagaagaa ctactagaaa gaatggtaga tgaatggcta cctattcaat tgaaagacat
    10981 gtcttatccc gaagaagctg tgagagaaat tactgataat ataaacaagc tagaacaaac
    11041 gatttcatca attgggaaaa aatacactaa gaaaaaaatg aagggaggtg ataccatcta
    11101 gtggtaagta ctccttgtaa actaagagga gatgatttaa tggcaaaaat atcaaagtac
    11161 aaaaagaaaa acggtcaagt ggcgtggatg tataaaggac acattgccac cgatccacga
    11221 actggtgaaa aaataaatac tacgagaaga ggttttagta gcaaacaaga agctcaaaaa
    11281 gattatgacg agtacagaca tcaaatatta tacggtgtta aaaagaaagc acctgatatg
    11341 acatttgaag acctgtataa tgaatggata actcatcagc gtaccagtgt taaagcttca
    11401 acaatcgcaa tctcagtaag atacgcaaat aatcaaatac ttccagcatt tggtaagctt
    11461 aaactatcga atatctctgt cccatattgt caaaaagtgg tagatgaatg gcacagtaaa
    11521 tatgaaagtt acgattatat gagaaaacag actgcacaaa ttttacgcta cggggttgca
    11581 atgcaatata ttgacaataa tcctatggaa aaaacacttc ttccacgaaa aaaagagtat
    11641 gagaaaaatc gtaaatttta ctcaaaagag gagcttaata atttactgga tgcttttaaa
    11701 gattttggta acatgaaaca atacgccttc ttcagattat tggcatacac aggtatgaga
    11761 aagagtgaag tactcgcttt acagtggaaa gacatagaca cgtttaataa agaactacat
    11821 gtcaataaaa ctcttgctgt agatgaattt ggtaaagtta taattcaatc tccgaaaaca
    11881 agagcttcta gacgagttat ctctttagat actgagactc tttctatcct caacaactgg
    11941 aaattacagc aaaaagaaga gtatttaaaa cttggataca atactagctc taaagagcaa
    12001 catgttttta caacagttaa aaacactcta tacattccaa acactgttaa tgattggtta
    12061 agatatattt tgaaaaaata taacttgcct cgtattacac ctcatgggtt tagacataca
    12121 catgctagtt tattattaga agcaggagaa tctgtcaagg tagttcaaca acgattaggg
    12181 catgaaaata gtaaagtgac attagacatc tatgcacata ttacaaataa tgcacctaag
    12241 aaaacaggtc aagactttgc tgatatgatg gctcatcagt aaaagagctg gtcaaaatct
    12301 ggtcatttct agtatacagg caaaacaaaa aagctagaac ccttatatat caagggttct
    12361 agctcctata acaggtttac ccaacactgc cttccatctc ataactgatc aatcgattga
    12421 gttcgactgc atattccatt ggaagttctt tggtgaatgg ttcaacaaag cccatgacga
    12481 tcatttcagt tgcttcagat tcggtcaggc ctcggctcat cagatagtag agttgttctt
    12541 ctgagatttt tgagactttg gcttcgtgtt ctaaagagac ttggctgttg tggatctcat
    12601 tgaatgggat cgtatcggat ttcgaccatt cgtccatgat gatcgtgtca cattcgatat
    12661 gagagatcga acccgcactg tttttggcaa aggttacttg tcctcgatag tttacttctc
    12721 cgccgtcttt tgaaattgat ttagagacga tagagctaga tgtatttggt gcattgtgga
    12781 tcatttttgc acccgtatct tggatttggt ttgctcctgc aaaggcgatg gagagcatgg
    12841 tgcctcgtgc accttttcca tctaagtaaa cacttgggta tttcatcgtt gtttttgcac
    12901 ctaggtttcc gtcgatccat tcaacggttg cgccttcgta tgcttttgct cgtttggtta
    12961 ctaagttgta cacattgtct gaccagtttt ggatcgttgt gtaacggcaa tacgcatctt
    13021 ttcttgtaaa gatttctacg atggcggcat gcaggctgtt acttgaataa gtaggtgccg
    13081 tacaaccttc gacatagtgg atgcttgctc cttcgtcaac aatgatcagc gtacgttcga
    13141 attgccccgt gttttccgca ttgatccgga agtacgtttg taatggaaca tcgactttga
    13201 cggcttttgg cacgtaaata aaggtcccac ctgaccatac ggctgaattc agcgctgcta
    13261 atttgttgtc ggttggcggt acaagttttg aaaagtactc tttgaataat tcagggtatt
    13321 ctttcaaagc agagtcagtg tcagtaaaga cgattcctaa cttttcgaat tcatctttca
    13381 tattatggta cacgacttct gattcatatt gtgcggaagc accggctaga taagctcgtt
    13441 cagcttctgg tataccaatc ttttcaaatg tatctttgat tttttctggt acgtcttccc
    13501 agtcacgagc gggtttgtca cttggttttt ggtaatattt gatggcatcg aaatcgatgt
    13561 ctgaaaggtc ggggccccat gtttgcattg gcattttgtt aaatgtttct agagatttca
    13621 gacggaattc aagcatccat tctggttcgc ctttgattct tgacatctca cggatcactt
    13681 cttctgtcaa gccttctcct gtactgaaaa ccggttcgac atcatcgtga aacccgaatt
    13741 tatattcttc taattctggt acgcccatgt tctcactcct cttccttgtg gtaatgatgg
    13801 aaagctgcgg taccatcgtt ttccattgct tttcctagtg ctttccaggc aagtgtcgca
    13861 catttgatcc gagcgggaaa tttagctaca ccaccaagca tagcagcatc gcctaattgg
    13921 tcgggatctt tgacttcttt tccttggaca agctgtagaa agtctacagt taaatgttct
    13981 gcttcttcta ttgattttcc gatgacggcg tctgtcatca tcgaggcact tgccgtactg
    14041 atggagcagc cactgccgtc aaaggcgata tttttgatga ttccgtcttt tacatcgact
    14101 tgcaattcga tcacatctcc acatgtgggg ttgttcaatt cgatcttgct tgtcgagtca
    14161 gtcaagcttc cacgatgatg cggatgggaa gaatgatcca agatcacttg acggtataaa
    14221 ttgtctaatt tagatagtcc catgttggaa aaactccttt gttgccatga ttgcttcaac
    14281 taaacggtcg gcgtcttgcg ttgtgttgta gaaataaaag ctcgcgcgag cagtcgctga
    14341 tacttcaagg tacttcaata gcggttgcgc acaatgatga cctgctctga cggctacccc
    14401 ttccatgtct aaagctgtcg cgacatcatg gggatgtaac ccatccaaat tgaatgcaac
    14461 gactcctgtc cgaaaattcg ggtcttgtgg accataaaca gtcaatccat caattgcttg
    14521 taattttggc aacacataag cggctagttc ttcttcatat cgatggatat tgtccatacc
    14581 gagtttttgc aaataatcga ttgcatggcc taatgcgata gcgccggcga tatttggtgt
    14641 ccctgcttcg aatttccaag gtagttcttt ccatgtactg tcgtataatt cgacaaaatc
    14701 gatcatttcg ccaccaaatt caacaggttc catttcttct aaccattttc gtttaccata
    14761 taaaacacca atacctgttg gtccgcacat tttgtgtccg ctgaaagcaa agaaatcaca
    14821 gtctagtgct tggacatcaa tggccatgtg cggggcagat tgagcaccat ctactacgat
    14881 gaccccgccg ttttcatgga caagttcagc taattcttga acaggattga agacacctaa
    14941 gacattggat acatgagcaa tggagacgat tttggttttg tccgtaatct ttttcgctgc
    15001 atcctccata tccaggaagc cttcttctgt tagttcgata tatttcaaga cagcacctgt
    15061 tcgttttgcc aactgctgcc acgggataac attagaatga tgttccatgt aagagagcac
    15121 tatctcgtct cctgctttga tatagcggtc gccgaaggtt ctggctaccc aattcaagct
    15181 tgtcgtcgta ccacgagtaa acagcacttc tgctgtttct tttgcatgga tgaacgcccg
    15241 tactttttct cttgcttgtt cataatcatg ggtcgcgcgt tctgctaacg tatggacacc
    15301 gcgatgaaca ttggcgttat cgtgttcgta atagtacgct aatgcctgga gtacttgttt
    15361 tggcttttgg gtcgtcgctg cattatccag ataaactaaa ggttcatcat tgacgtcttg
    15421 aaataagata ggaaaatcat tgcgtagctt tttagcatcg atcatccatt caacttccct
    15481 tcgattactt cgactaattc tttccgaact tgttctacag ggattgctgt taagacagaa
    15541 ccgaggaatc ctcgaatcac taagcgctct gcttcttctt tatgcaatcc tctactcatc
    15601 agatagtaca tttcttctgg atcaactcgt cctacactgg cagcatgtcc agcagttact
    15661 tcgttttcat cgatcaaaag aataggattc gcatcaccac gtgctttatc agaaagcatc
    15721 aagacacgac tttcttgttg ggcatctgca ccttttgccc ctttcaagat gtggccgata
    15781 ccattaaagg tcaatgttcc tttttctcgg atcactccgt gttgcaaaat atgcccgatc
    15841 gtatgagggg ctttattggt tactcgtgta tcgattcctt ggatttgtct gcccgagctg
    15901 attgccacga ttttgacttc tgcgtgtgca ccttctcctg ctaaatctga gtcaaagtca
    15961 gcgatcacat gaccgtcatt catgacacca attgcccagt cgacagaagc atctcgtaaa
    16021 atgtgcccac ggcgattcat ataagaagta atattttctc ctaattgatc tacggctgaa
    16081 tatttgattt ttgaaccagc tttagcgatg acttccacga tgatattacc tgatgatttt
    16141 gctacttgtt ctttggttgt ctggaaacgt tccagataag aaaattcact atgttcatct
    16201 gccacgatca acacatgctt gaagaagtgc tcatcggatg cgccatcttg aatgaataag
    16261 gactcgagtg gttcatcgat cacgacattt ttaggcacat acaggaaaat accgctgttc
    16321 ataaaagcta catggagtgc agtcaattgg tcttcgttca tgtttactgc ttttttcata
    16381 taatattctt ggactagttc tggatattcg atcattgcag tgaacaagtc ggtaaaaatg
    16441 actccttttt ctgctaattc cacaggcagt tgttcaaaaa tcgtccatga accttgttgg
    16501 acaagcacag gattgtcttt cattgcatca aatgccggaa tcgtgcctgt ttcactgatg
    16561 gtttgtgttt ctttcacatc aaataacgac cagcgatgga atttcacccg atcgatatgc
    16621 ggcaatggca gttcatctgc tttcgctagt gcgtcttgac ggaaggttct catccaatca
    16681 ggttcgcctt tacttaaaga aaaatcattt accgcgtcaa gataatcgag ccagtttttt
    16741 tctttcattc ttgctccccc ttacgcttct tcttctttat agtcgattcc taattcttta
    16801 ctgataccag cgtagccttc tgcttccaga cgtttagcta ggtcagcatt ccctgtcatg
    16861 actactcggc cttccatcat gatgtgaacg acatcaggtg tgatatagtt caataatcgt
    16921 tgatagtggg tgatgatcaa tgcaccgaaa ttgtctccgc gcatttcatt tactccttta
    16981 gcaacgactt tcaaagcatc gatatccaga cctgaatcga tttcgtctaa aattgcaaat
    17041 gtaggttcta acatcaacaa ttgcaggatc tcattccgtt tcttttctcc gccagaaaaa
    17101 ccttcgttca gataacgttc agccatttct tctggcatat ttagtaaagc cattttttca
    17161 tctaactttt tcaaaaatgc cataacagaa attttgtcgt tttcatcacg tttcgcattg
    17221 atcgctgcac gcatgaattc cgcattcgtg atacctggta tctcacttgg atattgcata
    17281 gctaaaaata agccaagtcg cgcacgttca tctacttcca agtctaaaac atttttcccg
    17341 tcaaacaaaa tttcgccttc tgttacttca tagtttggat tgcccatgat agcggcagat
    17401 aaggtagatt ttcctgtgcc gttaggtccc atgatggcat ggatttctcc ggttttcatt
    17461 gttaggttta cgcctttaag gatttctttt tcctcgatcg acacatgtag attttttatt
    17521 tctaagacag acatgcatta tacccccaaa ttctctttcg ttctcactca ttttagacta
    17581 attgagaatg aagcgcaatg accttcctta ctaaaatcaa atatctttta tttattatta
    17641 aacaattttg atttctaaat tagaataggt ataaaaaaga tgagaaataa gccattacag
    17701 acttatttct catctttttt gttccatacc ttattttgca tcagccttta ctggtttaac
    17761 agagccgccc catttgtctg tgatccattc ttggacttct ttgcggtgaa gcacttcaat
    17821 cagttttttg attttgtcac tgtcttcatc acctttacga gtcgcaataa tattcacgta
    17881 aggagagttg tccttttcaa gcaatacggc atcttctaat ggattcaacc ctgcgccgta
    17941 agcgaagttt gcgttgattg caactaggtc accttcatca ttttggtacg tagaagctaa
    18001 aagtgatggg tcgatcgtat ggttgaactt caagttctta gggttttctt caatatcatc
    18061 gaatgttgct gtttccagat ctactccatc tttgactttg actaagccag catcttgtag
    18121 aattgtgatg atgcgtcccc agtctgattc agaatttgaa gtaatgattg ttgcaccatc
    18181 ttttaattcg gaaacatctt tgactttttt agagtacaag cccattggtt cgatgtggac
    18241 agcaccggca ttgacaaagt catatccttt ttctttgatt tctttgttca aataaggaat
    18301 atgctggaag taattggcat caatgtcgcc ttcttccaaa gattggttcg gtaatacata
    18361 gtcatcgaat ttgacgattt caagatcgat tccttcatct ttcaataatg gtttcacatg
    18421 ttccaaaatc tctgcgtgcg gtgtcggtga agcgccgact ttcaatgtcg tgtcgtctgc
    18481 tttatttgct gaatctttgc tgtcgctgtt gttaccgcat gctgctaatc ctacagtcag
    18541 caaaagcgct gctgcaaatc caaaaatttt ctttttcata tcctacatcc ccctatatcc
    18601 tattatcttt tgtctgtttt cttagttaag aaatcaccga ttccttgaac gataaatacg
    18661 attagtaaaa taattaaagt cgcaacgaat gtcactgtta gattaccacg ctggtagcct
    18721 tcctgccaag caagacctcc aagtccacct gcgccgatcg cgccggccat cgcagtaaat
    18781 ccgatcatcg agatcgttgt aactgttaat ccagaaatca atgcgggaat actttcaggt
    18841 atcaagacct tccagatgat ttcccagtaa cttgctccca ttgcatctgc tgcttccagt
    18901 acaccagggc tgacttcacg aaaagcaatc tctactagac gggcataaaa tggtgctgcg
    18961 gataaaacaa gagctggaat ggctgcttgc gcacctagca tagtgcctac gattgcttta
    19021 gtaaatggaa tgatcaaaac catcaggatc ataaatggcg ttgaccggaa aatattactg
    19081 atgatcgata cgattccata aatgattcga gcatacggtt tgtttttgcg tccaatagag
    19141 taaaggatca atcccaaaac taatccgatg atcgtcacta agaccattga gattaatgtc
    19201 atatatagtg tatcaaggat agcctgattc atcgtttccg ggttgatttg tttaaaatct
    19261 agatatgttt ctgaaaagct tttatccacg atggatcacc tcaattccta ctccttgttc
    19321 tttgatgaac acttctgctt gatctaattc tttggtttca ccagtcatca agacagtcaa
    19381 tgaaccaaag gaaccttctt tcacttgttt gatctttcca tacacaacgt tgacatcgat
    19441 gttatagcgt cgaatcgctt gggaaatgac tggctcgttg gcatttcttt cgctaaaggt
    19501 caaagttgcc aaacggccgc ttggattttc tttgatgagt tcagctaata gttctgtcga
    19561 atcagcttct ggttcgatgt cttgttgtac aaagcgtttg gttacttctt ttttcggttg
    19621 tctgaagaca gtcaatacgt ctccttcttc tacgactttc cccaactcca tcacagcgac
    19681 tttgttgcag attttgcgga tcacattcat ttcatgtgtg atcaagacga tggtcaaatt
    19741 caatttctta ttgatcgcta acaataagtc taatacttct tctgttgttt gcgggtccaa
    19801 tgcgctagta gcctcatcac aaagcaggat ctcaggatca tttgccaatg ctcgtgcgat
    19861 accgacgcgt tgtttctgtc cgccggaaag ttgtgaagga tatgcttgtc cgcgtccttc
    19921 taacccgact aaacgcaaca gttcttctgc ccgttcttta cgtttgctct ttggtacgcc
    19981 agctaattcc agcggcagct ggatattttc aagtacagtt cttgaccata acaggttgaa
    20041 atgctggaag atcataccga ttttctttcg gaatgtacgt aattctttgt tttttaattt
    20101 tgtaatatct tgatcgttta cgatcacttc gccgctcgtg ggaagctcaa gtccattcag
    20161 gagacggact aatgtacttt ttcctgcgcc agaatagccg acgattccat aaatatcccc
    20221 tttttcaacg tttagtgaaa cgtcattgac ggcttggatg tttccgtttt tcccattgaa
    20281 tgtctttttg acattcgtta gttggattaa tgacatgcca ttcacatctt tctcttatga
    20341 tttttcaaac aaaaaagcct tcgccctctt gccttaagca aaaggacgaa agcttgaact
    20401 tccgtgttac caccttgatt taccgttact tcgcaataac agcctcttcc agtacttgga
    20461 cttagcttct ttccgtatac tgtcgcgctg taacgggcac tcccgtagta agtttgctta
    20521 cgctcgctta ctctgctcta agaccatctt ccatctcatt ttcgttaccc attttcagca
    20581 aacagggctc tcttcaaacg tccgtgacat gtactcttct tttcaaagct ttctcgtttc
    20641 atataggatt gcttatgatt atagcaactg gaagaatcag tgtcaacttt tttcaagtga
    20701 ttttctgatt ttataattcg tcaaattctt ttggatcaac atctttgaaa cttaagatcc
    20761 acgcattcat ttcatctgaa tcattcaaga tattgatatc ttcaccgatt ttatcattaa
    20821 cagaagaaac tgtacccgtt agcgggctgg caaattcatt gaccgctttt tccgcttcaa
    20881 cttcgatcag aggttcatct ttttttagcg attgtcctgc ttttggcaga ttggcaaacg
    20941 tgatgtctcc aagttcttct tgcgcttcat ttgtcaaacc aacacagtat tcttttccat
    21001 taaataagat ccaaaggttg tcctttttct tcaaatgctt gcctgccatt ttatatcagt
    21061 tctccttcat aaattttttc attgaatcca attgcaacaa gctggtcctc tcgtaagatc
    21121 agtggtcgct tgatcagcat accatctgtc gataagactt ggctcgcttt tgaagccgta
    21181 aactcgccta cttgatcttt cagtccaagt tcccgatatt tcatcccgct tgtgttgaac
    21241 aggcgacgga ttggtacttg actcgtttct agccaagtct caatcgttga ggccttcggt
    21301 acttctttga tcatatcaac ggtttgataa ctaacgccat gctggtctaa ccacgctttg
    21361 gcttttttgc atgttgaaca tttcgggtac cagtaaaagg tgtacaattt tcttacctcc
    21421 ttgaagatct tgcaggtgct tcattttggt atctcatcga taatatcaac ccgataccga
    21481 tcatgttccc taaaatcgag gatcctccct gactaatgaa aggcaatggg atccctgtta
    21541 aaggaagtaa tccgatattt gctccaatat tctcaaagac atggaacagc agcatcatga
    21601 tcaaaccgct tgcgatatag gcgtaaaatt cattattcgt atcgaaacac acccgaatca
    21661 tacgatagat taaaataaaa tacaagaaaa tgacgaatgc accaccgata aagccgaagt
    21721 tttcaccgat aacggtaaaa atcatatcgg actcacgaac aggcacatag atattgctta
    21781 cgttgaatcc tttaccaaac aagccaccgg taccaattgc caaaagtcct tgggccggct
    21841 gataactcat tcctgacgta tcatggaaag ggtccagcca tgaatcaata cgtgcaaact
    21901 gataagattt gaatccaact ttataaagca agtctcttcc gccttcagtc gtaaccaaga
    21961 agatcgttcc tgctccaacg actactgcca agattactac aggtgcgatg atgcgccaag
    22021 agattccaga catcaggaaa atcccgccaa agatcgcaag aaagacaagc atcgttccaa
    22081 agtcatcttg ggccatgatc aatgctaaaa ccggtattgt cacgatcaag agcttcccaa
    22141 tcagccaaaa gtccgatttc atcgtccgtt cttttacttg cgtattatgt ttagttacga
    22201 tcagtgccat catcaagatg tacgcaatct tcatcaactc tgctggctgg aaagtaaacg
    22261 caccgaatcg aaaccagttt ttagaccctg ttgaagcagc taaattctga tcatagaatt
    22321 tgagcaagag tcccattacc actaacccca aaccataaag gtaaggagtc agtttccaga
    22381 tccatttgga attcaaatgc atgatgacaa cgattgctgc cgctccaact aaataccaga
    22441 caacttgcat ccctacgcct ttcaagactg ctggattgtt cggatcatgg ctaagtgcta
    22501 catataatga gagcagacca atcaaacata acagaaagac tggtaaaatc acgccataat
    22561 caatacgatt atcgttcgaa gcttttaatt ttttttccat gtttcatccc ttttcttaaa
    22621 aaccatactg ctgattatta tagcggtcca gttagataat ggaaagtctt tccatagaca
    22681 tttgggaaaa attaactttc cgtttgtgca gttgcttgag caaatgcagt acaaaagcct
    22741 actgcttatc tttagtgtat catagaactt tttttgatac atgcaattat ctaccagctg
    22801 agattctcct cgctgtgaga agtttctgtg ctttcattgt atcgaaggat ctcttcggca
    22861 atctctagca gctgatcaga aaccagattg gttcgttgga tgaaataaaa atatcgatgg
    22921 aaattcaacg gagtcattgg cacattttct ggaacagagc gcttagacaa aatcgagcga
    22981 ccgattcctt ccttcagcaa cgcaacaatt acatcgttac ttttcacgat catcttctta
    23041 tcttggatat tcatctcttc aaaataacgc tgcgtgtaat gaaaaacacc agagtttgct
    23101 tctcttacaa gccacaattt gctttttggg tccccagcga tgactagctg gtcttccatc
    23161 aaagagtatc tttgtacgcc ggaagtctct aatggctttt cgataaaacc aaaatcggat
    23221 tcatgtttcg ctacttgttc taatgattca tgagaattcg tgatttcgat ctcaaaatgc
    23281 actttaggaa accttttgat cagatgtttc atcaactgag gcaaaagata gacagcaaag
    23341 gtgtgggaag caacgatccg acatacttct tcttcatttt gttcattgag caatttttgc
    23401 tgagtttctt cccattcttc taacaaaacc agtgtccgtt catataataa gtcggcttgc
    23461 ttagttggaa gcatctcttt tcttccgtta cgttgaaaaa gatcgatatt caattcccgc
    23521 tccaactgtt tgatttgatt cgagactgct ggttgagata aaaacagtac ttctgctgct
    23581 ctggaaaaat ttcttgtttc atatactgct cgaaaagtcg ttaataactt aaacatggca
    23641 ccacttccat aactttttat gataattact ataaaaacta tttgatttaa atatacaaaa
    23701 aaccatctat aatagaaatg tacaacaaaa cagttgctga tccaactagt aacttagatg
    23761 aagacaatca gctgtctgct tgctcgtaat catacattcc tattcatttt acaatgtatt
    23821 cgttcatctt ggtactaaaa tctattaaat aaaaaggaga atattatata tgaagaactt
    23881 tttgaaaatt attccgatcc cgatctgcgg gttgatcctt gggttagctt cccttggaaa
    23941 tctgtttaaa gactatcacc atgttgcttt aggcaatcta actggcggta tctctatgat
    24001 tcttatgata ttcatttttg ttaaattgtt tatcttattc gaacacacga aacaaacgtt
    24061 gcatgatcca atcatcgcct ccgtttcacc aacttttacg atgtcactca tggttatttg
    24121 tacttatttt gtttcatatg aaagtattgc accaattgtc aaattcattt ggctgtgcgc
    24181 tgttatcttc caagtgatcc ttgtctttta tttcaattat caccatgtta tcaaagctga
    24241 tctttcaatt gaagcaattt atccaagctg gtttatcatt tacgttggat ttggggtcat
    24301 cactgtaact gccgggaact tcttcccact tattggaaaa attttctttt ggatttcatt
    24361 gatctgctat gcgttcttat tgccgatcat catcaaacgt gtctttttcg taagaaatat
    24421 ggctcatccg acacgtccgc tgattacgat cgttgcagca cctggttctc tttgcctaac
    24481 aggttatctt aaaaactttg ctgaacctaa tttttatctt gtcgtagcat tgttcgcttt
    24541 atctcaagta ttgtatttcg ttgtgctgtc tatgcttcca cgtttgttca agcttcgttt
    24601 ttatccaagc tatgcagcct ttacattccc gcttgttatc tctgcaacag ctatatttac
    24661 tactgttcat tacttcaaca gtttaggtat ctatagtagc gtattggatt tcttaaaagt
    24721 tgcggaatgt ctgtttgcaa gtgtagttgt cttctacgtt cttgctcatt acgtacgttt
    24781 cttaattaaa gaacatagca aacatggaaa atttgtttct aaaaaaatcg cttaataatc
    24841 gattcaatag gttgtgaaaa agctgttctg catcaagtaa taagaacggc caaacaaaaa
    24901 cagctgttca tgtttttgta ctggttgggc ttattaccgc agatgcaagc ttttgaacac
    24961 cgtttaccaa gaggtcgtaa aagaagtggt cagcttcaag aaataagaag gaatttacga
    25021 aaattgcttc tcaaattttt gtgaaatttc ggcttatttc cgtagaagct acttctggaa
    25081 caccgtttat tcgaaacaag aggtcgtaca agacttttgt cacgacctct tgttcttttg
    25141 tcaaaatact ggtttagtgc tactatgttc gagtatgctt ttagtattta ttgacgaaag
    25201 gaaccgatca aatgaaatca ttgtcacatc caataaaatc tgtaaaggat tcagcggtcc
    25261 gcgaaatctt acccggttta ctgctttctt ttgccatcgc tgccttcagc aggcttcttg
    25321 ctatttatct gccacagtta ggtggagcga ctattgctat tttattgggt attttattag
    25381 gaaatacttt ttttaaacag cctgttctag caatcggcac aaaatttgct gaaagcaaac
    25441 tattagaatt ctctgttttt ttactaggtg caactgtcac agctcaaacg attgctcaaa
    25501 ttggtgtaaa aggaattgtt tttgtgttac tgcaaatgtc agtcactatc atcggtgctt
    25561 acttgattgg taaaaaactg caattcagcg atacttcttc tttgatgatg gctggaggaa
    25621 acgctgtttg tggttcttct gctattgctt caatcgcccc ttccatccat gcgaaagagg
    25681 aagaaaaagg acagatcatc acactagtca atctactggg tactgtgatg atgttgactc
    25741 tcccttttct tggattacag ttgtttggtg gaaatctcat tgaaaaaagt gcacttttgg
    25801 gtggtacact ccaatctgtt ggtcaagtag tcgctggggc aagcttactt gacaattctg
    25861 ttgtcgaatt cgccatgcta tttaagattc tacgtattat gatgctcgtt gttgttgtct
    25921 atctgtttga aaaattcatg cacaagaaac aaccagctct gtttgaaacg ccaactgata
    25981 cgcagaaaaa acgaaaatta ccgcttcctt ggtatgtatt agggtttgtg atcttttgtt
    26041 tggtcaacag cttgttttct ttacctgtag ttattgacca atcggcacat tttatcagca
    26101 gctggtttga gatcactgct ttagcggcaa ttggtctgcg tttagacttt gcaaaattca
    26161 tgaaagaagg tacacgcttc ttatcttatg gattagctgt cgggcttctc caaacagtga
    26221 tggctttact atttatttgg ctgatcaaag tttagaatga acatgaaaaa acagttgtga
    26281 ttctcgcatg gaacaatgtc cgaatcacaa ctgtttttaa ttttgcagat atccttctaa
    26341 caacaacaat tggcgtttga tttcaacccc atctattgcg tttcccatat atcctgtcag
    26401 ttttccattt tgcccgatca ctcgatgaca aggaataacg attggcaatg gattggttcg
    26461 atttgcctgc ccaatcgctc gcacagcttt cggactatca acagcaacgg ctacttcttt
    26521 atacgttcgg atttctccat aaggaatggt cgacaaagct ttccagacat tttgctgaaa
    26581 aggcgtacct ctctttatag ataaaggaac atcaaattct tttctttttc ccgcaaaata
    26641 ttcttcaaac tgttcttgtg tgcgcgttaa aattcgctga ttcgctgatt cgctttctga
    26701 aattggctga aaagatacag cagtcaatcc ggattcatct gcatctaacc ataaaaagcc
    26761 gataggtgtg tgtattttca ttttgtttgc tcctagtcaa tagactggaa cttcctcctt
    26821 tttctctatc caaaactact tgccaactaa taaaaattgt tcttaaaagt ttgataaacc
    26881 gaggaaacat ggtaaactaa atacgttagt ttacaaaaag aatacctgtt cttttacgta
    26941 aaatcaaatt ttaaaggtgt tatttaaggt ttattaaatt ttgaatgtaa accatatata
    27001 aacatacgat tgaaaggata gaaacgatga aagtatcaat tgttgtccca tgttttaatg
    27061 aagaaaaaac tgttcctctt ttttttgatg aggttgaaaa attcaaaggt gcccatgagt
    27121 tcgaatattt gtttattgac gacggatcaa aagacggtac gttagcagca atcaagaagc
    27181 tagcctcagc ttatccttat gtccgctttg tctcattttc cagaaatttt gggaaagaag
    27241 cggctctgta cgctggattg caagcttcta caggagattt agtgaccgtg atggatgttg
    27301 atctgcaaga tccgcccgaa ctgcttcctg agatgatcaa ccggatcact acttctgata
    27361 tcgattgtgt cggcacccgg cgttcgactc gtgaaggcga gcctgcgata cgcagtttct
    27421 ttgcgaaaaa attctaccag ttgatcaata aaatcagcga tacagaaatg gtcgatggcg
    27481 ctcgtgattt ccgcttgatg actcgtcaaa tggtggatgc cgtcctagaa ttaacagaat
    27541 acaaccgatt ttcaaaagga ttatttagct gggtcggatt taaaacagaa tatatttctt
    27601 atgaaaaccg cgaacgtgtg gctggcgaaa catcttggtc cttctggaaa ttattcaact
    27661 actcgattga tggtatcgtg aacttctcgg atgcaccgtt aacgattgct tcatttatcg
    27721 gcgctttgtc ttgtgtcggt tctggtattg caattttgtt cattattttg cgggcattgt
    27781 tgtttggtga tcctacatca ggatggcctt ccatggtctc gatcttcctg ttcattggtg
    27841 gaatccaatt gttatgtctt ggaatcatcg ggaaatatat tgggaaaatc ttcttagaaa
    27901 cgaaacagcg tcctgtttat atcataagag aaacagaaaa agtgaagcca aatgaattga
    27961 agcctcacca ataatatacg tcaaatgaag taaacatttt tttaaatgaa aaaggactgt
    28021 gacaaaagtc ttgtcacagt ccttttttcc gaataaatgg tgttccagaa gtagcttctt
    28081 cggaaataag ccgaaatttc acaaaaattt gagaagcaat tttcgtaaat tccttcttat
    28141 ttcttgaagc agaccacttc tatcacaacc tctttcatat cttattactt cttcattgct
    28201 gcttttagtg ctgctgctaa tgggctttct tctggttctt ctgcttgtgc atattttttc
    28261 agcaagcgtc gttcttcatg tttggtcatt ttcttcttct gctctttttt atcaagcaat
    28321 ttttctgtcg ttccatcgta tttacaacgg aaataagcac cgtttttacc ttcgatgatt
    28381 tccattttcc ggtgacattg cgggcatcga tgattagaga cttttggatc ttttcttctg
    28441 cggtagttgc aatctgaatt ggtgcagaca tagattttgc cgtcacgtgt gtttttctcg
    28501 cgcagattgg ccccacattc tgggcatttc ttttgggtaa tcgagaaatc ttgatattct
    28561 tgtttgcttg atttgatctc attcaccaag cgttttgttt ctttttcgat tccattcaag
    28621 aaggttcctg cttgtttctt tcctgcagca atagcttcaa ggtctttttc ccagctttct
    28681 gtcaaatctg gtgttactaa agagggattt accaatacta ataattgctt acctttagga
    28741 gatacttgca gtccttgagg ggtacgttcc atcagttcag atttgattag tttctcgata
    28801 atctctgcac gcgtagctgg ggttcccaga ctgtattttt ccatttgacc aagcaagctt
    28861 gcttctgaca atggtttagg cggtgcagat agttcttttt tgatggcgaa atcgggattg
    28921 attctcattc ctttttccca tttgactgtt tccgtctcag tttcagtttc gcctttccat
    28981 cctgcttgga cgacacgatt ttgccgaaaa atgaatcgtt ctttaccaaa ttcaaccgta
    29041 acttttgttt ggctgacttt atggggttga gcaaacagtc ctaaaaaacg agagacaatc
    29101 atttgataga tttttcgctc ttcattactt aatttttcat aacgtgctct gttttcagtt
    29161 ggcaaaagtg catggtgatc ggtcacttta ctgtcttgga atacttttgt ctgttggacg
    29221 atggctccgt ttttcaaata ctgcttgacc tcaggagcaa agtcgctgac tgcttgcaat
    29281 cgttctttca tcgtactttt catgtcattt gtcagatatt tgctgtctgt acgcggataa
    29341 gtaacgatct tatgggtctc atacaaactt tgtacaagac ttaatgtttt ctttgcagaa
    29401 tagccatagc gttgattggc ttctctttgg atctctgtta aatcataagg caatggcgca
    29461 gattccgttt ttaatttttc ttggacttct gtgacagttc ctttgcttgt actgatcaat
    29521 tgaacaagtt tttgtgcttc ttcttgtgtt tttaaagcaa attgattttt ttgaagcatt
    29581 ttggcttgtt cttttccagc ttgcaacgtg atcgtgaaat acgtttgtgg acggaactgc
    29641 tcgattttct tttcttggtc tctcactaat gctagtgtcg gtgtctgcac acggcctgct
    29701 gataaattat ccgcgtactt cacagttaag gctctggtca cattcaatcc taccagccag
    29761 tccgcttttg cccgggcaag agcagattca tacaatgcat catattcttt cgcaggtctt
    29821 agttgcttga atccagtttt gattgcttta tctgtttgag aagagatcca taaccgcttc
    29881 actggtttat tgaaatgcac atactctaag atccagcgag caacaagttc cccttctctt
    29941 ccggcatctg tcgcaatgac tgcttctgag acatcttttc gctgagccag ctgcttgatt
    30001 gcttttaatt gatgtcctgt tttaggaagt ggtttgattc ctagtttttt agggatcatt
    30061 ggcaacgtgt ccatttgcca gctctgccat tccttattca gatcttccgg cattttcaat
    30121 cctaataaat gaccaagggc ccaagtaacg atcaccttgg gtccttcata ataatttttg
    30181 tgcttttgat tagcaccaag caccttgctt aggtctttcg caacgcttgg tttttccgct
    30241 agaattagtt gtttcataaa attcctttct gctttctact gtgatgggct gatgattttt
    30301 caacaacgca agtcccttgt attgctgtaa atcatcgtca cactcttcac accagcgctc
    30361 atctccgtca ttccaaaagg cgctgagaaa gttacttttt gcattgatat attcataatt
    30421 ttgatggatt tcgttccatt gtttttcgta cgcttgtcgt gcttcttcga aagactcgaa
    30481 tgtttcttct tcttcaatat cttcttccca gtcttcaaaa aaccaccaag gctcattgtc
    30541 cccgaacatg gtaattactt gatacatact cgtgctcctt ctttcagctt ttctagactc
    30601 atcttagtca cttttcaaga aagttgcaag aattttctct tataaaaggg tcatttaatg
    30661 aacatcttaa aattgttaca aatgaatctg attgtttaag gcaaagtagc gataaagtta
    30721 ttgatcatcc aaaaatagct gtcttctgga tcgattgttg gttcttttgt aaagtatcct
    30781 aaaaaacgta aggaaagata accgtggatc atactacgaa gttccctgct tcgatgaact
    30841 ttttcttctt tcgttaattg aaaagcctca aatatctgca aaatgatctg attcgtttca
    30901 tggattccat ctaataaaac agcttcattg gtatgcggta cactgatcag taactcatac
    30961 actgcttgat ttgaaaaagc aaaggatcga taagtttctg catagatctg cagtgcttcc
    31021 tgaccacttt ttcccacaag agcccgtcgc agctggtcat taagttcctg cagaagtttc
    31081 gctgttagcg ctgttttgac atcacgaata tttttgaaat ggttatacag cgagggagcc
    31141 ttgatatcca agcgtttggc taaatgctga aaggttattt gctgaacatc catctcttct
    31201 gctagttctc gaaagcagtc gatgattttc tcttgagaca gatttctttt tttcatttcc
    31261 atgtttcctc tcataaaata aatccttcac tctttcagtt taccacgaaa aagaaagaga
    31321 aaaaagagac tttcattgat aaactacata ttgtagttta taataaagag ctgtagatga
    31381 aaattttcag aaagaaggag tctaattggc tgcaagaata aaggaaaaat tatttaggaa
    31441 aagagaaact tgggaacaaa acttgtttgt cttatggttt ggaacattta tggctggtat
    31501 cggatttagt ttagtcatgc cttttatgtc actttacata gatacgttag gtaattatag
    31561 tacgtcacaa ttgaactttt ggagcggatt gactttttca tctactttct tggtgactac
    31621 gcttatctct ccttggtggg gaaaattagc cgaccaaaaa ggaagaaaat tgatgctgtt
    31681 gcgtgcttca ttaggaatgg caatcgtcat ttctttgatg ggttgcgtga ctagtgtgta
    31741 tcaattagtc gcattgcgtc tgcttcaagg gattttttct ggttatatca gtaatgccac
    31801 tgcccttgtc gcaactggga cgccaaaaga aaaaagtggt caagttcttg gaaccttagc
    31861 aactggttct gttacaggaa cattacttgg accgcttctt ggcgggatct ctgcttcagc
    31921 gtttggctat cgccctacct ttttcattac agggatcatc ttatttttcg tcttcttgtt
    31981 gagtctgttt ttcgtccatg aaaaatttgt cccagtgaaa aaagaaaaca tggtttctgc
    32041 caaacagatt ttcaaagact tgaaatatcc acacgtcgtc atcggtatgt tcgtcacaac
    32101 gatgatcatc caagcatcca acaattccat tagtccaatc atcagtctgt acattcgcca
    32161 attgctgcat ggacatggga atgttacgtt gatcagcgga atcattgctt cgatacctgg
    32221 gattgccact ttgatagccg ctccaagatt tggtcgctta ggtgacaaga ttgggagtga
    32281 aagaatactt gcgattggtt tgggttttgc gattctggtt tatatcccaa tggcattcgt
    32341 cacgaatgtt tggcaattag ctgccttacg ttttttgatt ggtatctctg atgcttgtct
    32401 gctccctgct gtccaagcat tgatcacaaa atattcgcct catcaagctg ctggacggat
    32461 cttcagttat aaccaatctt tccaagcaac tggtaacgta gtagggccta tgatcggctc
    32521 cagtgtttcc agtgcatttg gttatcgcgg cgtctttctt tcgacttctc ttcttgtttt
    32581 ggtcaacttc ttgcttgtac gacacaatac acaagagatc catcagcaag aaactgcaga
    32641 agaaaaagct tcaggagtct ccatagcttc agggcactct ccttctcata gacaataagc
    32701 gtaaatgaaa aacactgctt gaacagttgt cacagactgt ccttgcagtg tttttgttta
    32761 ggcctgtttt ttcaagaact cagataagag gaataatccc ttttccaagg tttctttttt
    32821 acaacagtaa cctagtcggg catgcttcgg taaatcaaag gcacttcctg gtactaatag
    32881 tactcctgtt tcttctaata aattgataca aaatgtctgg tcatcttctt cgatggctaa
    32941 tttaatgaaa gaagtagaga cgtaattggg agcgacaagt tcaactttcg gttcattggc
    33001 tacccattgt tttaaaatca caaggttctc tgtaatgatc tttttgtttc gcgccaatat
    33061 tttttcttta ttcttcaatg catgtacagc aagatcatct gacaggactc cgccgcaaat
    33121 catcgtgtag tctcgatact ttcgaaaaac ttctgccaac tcaggtccag tagctgtcca
    33181 gccaatacga atacctggaa tggagtaggt tttggatagg gaattggttg ctataccttt
    33241 ttcatagaca tctacgatcg acaagaattc acctttatcc gttaaaggcg catatacttc
    33301 atctatcaat acatatgcat ctactgtacg agcaatttct gcgatttttt ctaaagtttt
    33361 ccgatctagc aatgtgcctg taggattgtt tgcactattc aaacagatga tttttgtttc
    33421 aggtttgact aaggcttcta gctgttctaa atcaaactgc caatcttctt cttccttcaa
    33481 atgaacaaag tcaacagcgg cacctaaaga tttggggatg tcatataatt gctgataagt
    33541 aggaagcatc gaaacaacat ggtctcctgc ttccactaac gcgtatagag ctaaaagatt
    33601 agcaccagtc gctccattcg tttgtaagat gttttccgga tcgacggttt gataaagaga
    33661 agcgacttct tgtttgaaaa catctgatcc ttcgatccag ccatagtcca atggttcgtt
    33721 tttgcgtttt tcgaaaaatt tacttacgct agtcccatct aagccaacga gttcgtctaa
    33781 tgtcaatgct tcgatcgagc tttgtgcaat atcatatacc gctttctttt cccatttatt
    33841 tagccattct tctacaccga aatttgcgat tttcatgttt ctttccccct tatatcttga
    33901 tccagcctac agcaagcaac ccgattgaaa gcagtgcggc cacacaaatc agcccaatca
    33961 ttactttttc ttttcccgta atttgatgtt tggtttcacg acaagcgatc cagtagaagc
    34021 aaaaaccagg aagataggca atactacata aaaggacata agaaaatcct gctaaaatga
    34081 tgcccatcaa ttcaaaaact acagcaatcc caccgatcac tacttgtttt ttgattcctt
    34141 cttttaaaga aaactggact tgatagatcc cgacaagtag ataacaaatc aaaattgccg
    34201 ctgtacataa agaataggca aattgatagg cataatcagt aactaagaaa gtcaataaga
    34261 atagattagt caagattgca gtaacaacta acgaataagt tggggcattt tttttattcg
    34321 tttttcccca acctttcggc aacagaccgt ctcgagccat caacatcgtt gtttctgctg
    34381 ggagcatcgt ccaagacaac caagaaccaa tgatcgaaat aatcaagccg atattgatca
    34441 gcgctgctcc ccaatttccc acgattcctt ttaaaatatt tgccatagca ggttgtgaaa
    34501 tagttgctaa ctcttcttga gataagacgc cgtatggcaa gatcgaagca aggatataga
    34561 taaacaataa acaaagcaga ccaatgatcg atgcttgctg ggcttctgaa cgtttcttcg
    34621 cgcggttggc taaaacactt gcgccttcga tccctacaaa gacccacatc ataaccatca
    34681 aacaaccttt aatctgcaac cagatcgttc cttgctgccc tgttccattc actagattat
    34741 cagatacgtt accccaaaaa tcagcagtga atatattcac tttaaatgcg ataaatacaa
    34801 atattaaaaa taggaataat ggcactaatt tacagatcgt cacgattgta ttgatgacgc
    34861 tcgctccttc aactccattg tttactaata aagtcagacc ccaaataaaa atacttgcaa
    34921 ataaaataga aggaacattt tgtccacctt tgaatatcgg gaaaaattct cccatggcgc
    34981 tcatcagcat cgttgcaaaa gcaacatttc ccagccacgc agacaaccag taaccccaac
    35041 cactgatgaa gccacctaat ttaccaaatg aagcttccgc gtaaccgaaa ataccaccat
    35101 ctagatctgg acgcttttct cctaaatgat tcagcgataa taccaaagct aaaataccaa
    35161 tcccaacgat tatccatgaa aggatcgctg gccctggagc tgcagaacta gccagatcac
    35221 ttgtgatccc aaacacaccg cttccgattg acgaactgac aaccagagca accagtcccc
    35281 atttgctaat acccgtcgat ttcttttcca tttccatcac cctcacttat aaaattgaac
    35341 aaatcacttg caaaagattc ccttctattt tgcaactgct cttgcttaac aaaaagcaac
    35401 tttattcata ttattggata ttatgaactt tttacacaag tatttcaagt ataaaatgaa
    35461 taaaaattaa ttttattgca aaaaaacagc tatgctatcc aaaagaaaag gtgtttcttt
    35521 tgttagccat agctgttttg attcatttaa tatcgcttaa acgtattgta aaacttttgt
    35581 ttcttgatat gcgtggtcga tcaaaccacc acccagacat tccattccat cataaaatac
    35641 aactgcttgt ccaggagtga ttgcacgtac aggttcgtcg aatacgactt ctgcacgatt
    35701 atcatccaat aagcgaactg tcacaggaac gtcttgctga cgataacgga atttcgctgt
    35761 acacttgaat tcttttggca ttggttcatt cgtcgtaaag tggatttcac ttgcatccaa
    35821 gcttgtggca tacaatgcag gatgatggaa cccttgcccc acatataatg tgtttgtcgc
    35881 tagatctttt cctacaacaa accagggttc ttgcgaatct cctccgccac cgatccctag
    35941 tccttgacgt tgaccaatcg tataatacat caagccagca tgttgtcctt tgacttcacc
    36001 atcaagagtg accatatttc ctttttttgc tggcagatag ttgctcaaaa attgtttgaa
    36061 gttcttttca ccaataaagc agattcccgt tgaatctttt ttcttagcag tcgcaaggcc
    36121 tgcgcgttca gcaattgccc gtacttctga tttttccatg ccgcctagag ggaacatcgt
    36181 ttttgaaagc tgttcttgtg ataattggct aaggaagtaa gtttgatctt tattattgtc
    36241 tactccacga agcatgtgga ccacgccgtt ttcatcgcga gtgacttgcg cataatgtcc
    36301 agtagctaca taatcggcgc caagatccat tgcataatcc aagaatgctt tgaatttgat
    36361 ttctttgttg cacatcacgt ctgggttagg tgtacgtcct gcgcggtatt cggctaggaa
    36421 atattcaaat acacgatccc aatattcttt ttcaaaattc acagagtaat aaggaatccc
    36481 gatttgagca gctactttcg ctacatcctt ataatcttcc gtggccgtgc atacaccgtt
    36541 ttcgtccgtg tcatcccaat ttttcatgaa tattccgatc acgtcgtatc cttgttcctt
    36601 taacaaaagt gctgttacag atgagtcgac accgccgctc atccctacga caacgcgtgt
    36661 tttgctgttg tctgtcatga tatcaccatc atttcaatta gattctgaaa catctacgag
    36721 ttgaaggtcg caacttttca gtattttaca ttatacgtca tatttctgaa aatttcatcc
    36781 cttaattatc aagtcgtaaa aatactgttc tactggacct attcagtaca caaacatgat
    36841 agtcaaccaa aaaatcactc atcttttgag cattcgatat aaaaaattgg catactcata
    36901 aaaagggcca gcctttcggc aataaggaat aattttataa attgtgaaaa actatttttc
    36961 aaaattgctt cttatcgctt aaagctgcac gtctcattta caaccttttc tttttagatg
    37021 ttatgagttg ctcagataag tgattttgtt tactctaaga aaaaagggag gcaaaagata
    37081 catgaattac tttatcagtg atatgcactt tttccatgaa cgcttattag gaaaaaacga
    37141 ctttgctccg cggctctttg caaatgtgga tgaaatgaac caacagttga tcactagctg
    37201 gaatgcagta ataaaagaaa ccgaccatgt ttatcatcta ggcgaccttg ctatgcatcc
    37261 gcaatatgaa aaagcaaatg cagaaatttt ggaactggtc actcaactgc aaggaacaat
    37321 ccacttgatc aaagggaacc atgacagtcg cgcttttttc cgttatttag aaaaaaatga
    37381 tcctaggctt actgacggaa gtcccaaatt ttatttttat gatgtaggtg ccatcgttaa
    37441 attcgaccat caacaatatt acttgactca ttatccgatg ttgctcgggc ctaacgacac
    37501 tatccgcaat cttcatggac atatccatca ttacagcgta cctatcgaga acgatgtcaa
    37561 tgtaggcgta gacgcaccgg aaagagaact cttaaaggaa aaactaccat ttggcactcc
    37621 gttatctgag caacaaatca aagaaatcta tgaagcaaag aggaagtacg ataggtcgtg
    37681 aaaaaactgc cctatatcaa gtattaaaca aataaagata attactcata tttttcattt
    37741 gagtaggctt attgccacag gaacattagg atcagaaatg gagttgtaac aagacttctg
    37801 tcacaactcc attccatact attcgtcttt ttgctcatgg tgttctttta ccagtttttt
    37861 ctgtaattct ttatcataat ttcgggcagc tgacgttaaa aataaaacga ttccgcataa
    37921 taaaatcccg attccggaaa acaagaacat tttttctacc cctaaacgat cagctaatgg
    37981 tcctgcaaat aataatccaa ttggacctgc caggttcatc aaagagttga agttccctaa
    38041 gacacgacct aaaacattcg attcataact ctgctggatc attgccatca gcagtgtatt
    38101 gaaatatggc gttgcacaac ctgctcctgc attcaagata agaaagtaca agaacccttg
    38161 actatcgttt ggaaccaatc cgcttgcacc gatcgtcagg cccataatga tgtaggctat
    38221 aataataggt ttcattcggt ctttccatgt tcctataaaa ctgatcagtg caccgcctaa
    38281 gagcattccg actgcgtaga ctacttccac tgctcccgcc tgtccaacgg aaccaccaaa
    38341 gtaatctagc gtcatcaatg gatacagact gattgctggc atgaacaaca gcatcacaaa
    38401 tgcaccattg atcgttatgt accataaccc tttattatcc atcaattgct gcagcccaaa
    38461 ttttgcatct ttaaaaatag taagcggaac ttccaatatt tcaggagaaa cttttgggat
    38521 cttcacaaaa atcaataagc cgacaccaaa aactgccccc aatacatcta ataaaataag
    38581 gtaattgaca ggaacgactg aaaacaaagc cgcacctaga gctggcgcaa tgatgaaatt
    38641 cgctgattgg accattccta actgtccatt cgtccttgtt atgtgagaag atggaacgat
    38701 tgtcggcata atcgactgga tcgttggcat ttggaatgtt tgtgcgaccg aacgcataaa
    38761 caaagacaca aaaactagcc aaatcgggaa ggattctgaa atcgttccga ctactgctaa
    38821 aataagcgcg aaaatcgcta caataatatc cgtgacaatc aaaagagctt ttttatccca
    38881 gcgatccact agcggtccta caaatgggct tagtaaaacc atgggaatca ttcctagtaa
    38941 agtagcaaaa ctcaaaattg tcgccgaacc ggtttctctt gtcaaatacc agatgatcgc
    39001 atattggacg accatactag tgattcctga caaaaactgt cccgataaaa agaacaaata
    39061 atttctcttc caattcgtta aaacgaccga ctgttcattt tccataaaca tccctctttt
    39121 ctttttcatt ttgtatagcg tttgcacaac aatcattata cgggattctt ttttcaaaaa
    39181 ataccttttt ccctaaataa caatcgtttc aatttaaatt tctttactta gaaacaataa
    39241 atcagcaaaa tattaaaaat aacaacatgt gttaaattta aaaataaaaa agacaactaa
    39301 tttgcgtcgt ccttacaaag ttagtcatct aacaataggc cgtttcaaaa ctagttgtct
    39361 ttttatatcc acagatcatt cgactttttc gaataaccca cggctaatca tcccatccat
    39421 cagaaaataa aatttatctt ggctcatttt ctgttttgga tctttgaaaa tattgactgc
    39481 tttcaatcca tttgctgtag tgatcgacat tttcaatgtt tgaatatctt ttgatacagt
    39541 gatttttaat ttgaatcctt ctttgctttg cggcgtggca tctaatggtc ggatcaattg
    39601 ataattgcct ccgtttgttt ctgtaaatcc attatcgcgc aatgtatatc tttttgcatc
    39661 aggatgcaaa cgatagctaa ttggtgcgcc cagtaccgtt gctgttgttt caaatgccat
    39721 gattttcacc tcgataaaat tagtttctct ttattataaa agtttatcct gatttttact
    39781 aggtttttca gttaagttcc ctaaaaaatg aaggcgttct ctcagacatc tgttcatata
    39841 agaatgactt cactagttat tttttgacag cacgaactaa ttgatcaagg aaatactgga
    39901 tgtcgtcttc ggtatttccc aaaccaaagc tgatacggat ggattctttt agtatcggtg
    39961 tatcttctcc atacattgct tctaatacgt gtgaaggctc gatatttcct gctgtacaag
    40021 ctgaaccagt agaaatagcg accccattaa gatcaagctt catcagtagc aaatcatttg
    40081 cttcgccaat taagcgaaga ttcaacacat gagggagttt attcgttaaa tcaccattta
    40141 cttgataagg gatttcgttt gcttctaatg tatcaagaat caattgctgg aatcctttat
    40201 atttgttgtt acgtgcttca cgttcttcag gtgacagcag ttcaactgct tttgccatac
    40261 cccagatccc tgctaaattt tctgtacctg cacgccgctt ttcttcctgc tccccgccta
    40321 aaagcaatgc agggatattt actccgtctc gtttatataa gaaaccgacg ccttttggcc
    40381 cattgatttt atgagcagag atacttaata aatcgattcc taattcttct ggatggatcg
    40441 tttggtttcc gtacgcttgt accgcatctg tatggaaata agctggatga tccgctaaaa
    40501 gttcaccgat ttctttgatt ggcatcaaat ttccgatttc attgttcccg tacatcacag
    40561 agaccaagat cgtatcttct cgtaacgctt ttttgacctc ttcgatcgac agattccctt
    40621 tttcatctac aggcagatat gtaacgtcaa acccctttgt ctctaaatat ttcatggtat
    40681 taagaacagc aggatgttcg atagctgtag tgattagatg tctgccttct ttttgacgcg
    40741 agaacgctgt ttctaagata gctgtattat ccccttctgt tcccccactg ttaaagatga
    40801 tctcgtgagg ttttgcttgc agactgtctg caatgatttg tcttgctgtt tccaattttt
    40861 catgcgcgtg gcgaccaaat ccatgtatgc ttgaaggatt tccaaacgtg tcttgcatga
    40921 ttgccgtcat cgcttcaatt acttgtggat gcatgggggt agttgccgca tgatccagat
    40981 aaatagtttt cacttcattc atcccttcca aattattgct aggcacattc tagcataaaa
    41041 tagcgaacaa accaaagaag agctcatttt tgtctagagg acgtttcatg ggcatagaaa
    41101 aaaggagctg tgacaaaagt cttgtcgcag ctccttgaag ctgctcactt ctgtcacaac
    41161 ttctttccct cttttttttg tttattcgaa ttctttttct tccagacgga ataacggact
    41221 catcggtgta ttttcatgga tgcgtttgac cgcttcaccc attaattctg aacatgtaac
    41281 gattgttaag ttttctggat gacgattttc ttctgtgaaa acagaatcag tgatacaaat
    41341 atctttgatt ggtgcattgt ctaaaatact tttcgcatga tcggacaata atccatgtga
    41401 cgcacaggca tatacttctt ttgcgccgtt ttcttttaat acatttgctg ctcgtgcaag
    41461 cgttgctcct gtgttcaaga tatcatctac catgatacat gtctttcctt gaacatcacc
    41521 gatcacataa ccaccctcag ttgaagcatc atctgcatga tctacgatgg ctaatgtgct
    41581 gttcaaatat tctgaaagac tacgtgcacg ctgaacaccg ctgttttttg gcgaaacaac
    41641 tacgatgtca tctcccgtca gacctaattg acggtaataa tgagcaaaca acggcatcgt
    41701 aaataaatta tctacaggaa tatcaaagaa tccttgtact tgaaccgtgt gcagatctaa
    41761 agtcagtact cgagttgccc ctgcttcttc gatcaagtta gcgatcaatt tcgcagtgat
    41821 cggttcatga ggtttcgccg tgcggtcttg cctagcatac ccatagtaag gcaatacgac
    41881 attgatcgtt ttcgcacttg ctcgcttcat tgcatcgatc atgatcagta gttccatata
    41941 gtaatcattg acaggtgcat tcgttgattg aacgatatac acatgatccc ctcgaatact
    42001 ttcttcgata ttgatggaaa tctcgccatc actaaattgc ttcacgacgc ttttacctaa
    42061 ttctgttccg aacactttcg cgattttttc agctaatgga cggttgccat tcaaactaaa
    42121 aatcttcaac gtgtcatcct gatacttatt actcatgtag tcctccccaa aacttctcta
    42181 ttcttcgata gtttaacata tttttccaag attttctgtt aatttcagca aattcttaag
    42241 gaagtgaaac tgttccgtta ctccaactgg caggaatgac tttatgataa tcgccccagt
    42301 tgtcgtgtac tttgtaataa cgttctcctg tttcagtttc aaaataagcg tacgctgtca
    42361 cccaatgatt gccatatgta ctgccaagta ctttcaaaat accaatcatc acaggtttgc
    42421 cttcacgtat acgttttgta gctcgctgcc atgaacctag aagagttgct cgtgcacgct
    42481 catgattgcc attcttcttt aaaaaactag aaatccctgt actaacctga aaaggaatcg
    42541 taggaagtcc tagtggttgg atctggctac gcaatgcctt cgtcaaaacc aacgaatcag
    42601 ccgtattttt cttgcgaatc tcatttggaa gcatccaccc cttacgatag tcttgataat
    42661 aggcaaggag aactgctgcc gcgtaagtac cacaaagata gcctgagggg gtagcccatt
    42721 tcttatacct caaaaaacgt tcttctggca atcccaccca gcaatttgcg agttcctttc
    42781 ctgacctttt ttcttcgatc aaaacgggaa tatcttcttt tctgttgatc catctagata
    42841 actcgattgg ctcttcattg atcagcttcc attttttaca agaaagaccc tcttgataag
    42901 tatattttgc tggagaatat tctaccggat gtcctgattg atcgataatc acataacttt
    42961 gatcttcaaa aaccagagct aaaaaaagca atttactttt tcttgactgc agctgctgag
    43021 tgctggctaa ttttttaatt tttttatcta ctttgaaata taaaggttta tccactactt
    43081 gattcagttg ggatcgcctc ctttttttca ttataagtga aggattgaaa aaaacaaatc
    43141 agtttgattg ttcgaatttt caatttttta tgttctattt gttaattcct tcattttgta
    43201 agataatggg cacacgaaac aaaaaaggag cgatcgatga atgaacaaag aacgattgaa
    43261 acaagatttc tatgaagcag tcaatgaaga atggcaaaaa acagcaaaaa tccctgcaga
    43321 taaaccagct acaggcggtt tccaagattt agttgacggg atcgaccaac ttttgatggg
    43381 agaaacagac gatctgcttg cccatccaga aaaagtgtct tcagaaaaaa tgaaacattt
    43441 ccttgcatat tatcaactgg caaacgatta tgaccgaaga aatgagttgc atgctcaacc
    43501 gcttcaacct ataatcgaaa aagttgaatc attgcagtct ttcgacgaat tgaataagca
    43561 atttccagaa tggctgttgg atacattacc attgccattt aatctggatg tagatgcaga
    43621 tatgaagaat gcgcaaacga acgccttctt tgcttatccg ccttctttat tccttccaga
    43681 taagacgtat tatgcatcag accatcccaa cggtgcacaa ctaataggcg ttttctttga
    43741 tatggctgtt cagttattag agcttgccgg caaagaaaaa agtcaggcag aagcaatcac
    43801 ggaacaagca gtgcaatttg ataaattgat tgctcctcat gtaaaaagtg cagaagaaaa
    43861 cgcagattac agcaaaatgt ataatccgca atcttttgat acatttatta aatacacaga
    43921 taaactggac ttgaaaaaac ttgtcacggg tttgattggg acaactccag ataaagtgat
    43981 cgtaactgat ccagtgtact tcgatcattt gaatgacatc ctgacagaag ctcattttcc
    44041 gttattcaaa agctggatga tcattaaaac agtacgttct cttagtggtt atctatcaga
    44101 agaattccgc caagtcagcg gaatctattc tcgcgtattg tctggaacag acgaagcaat
    44161 gcctcagaaa aaagcagcgt attaccttgc ctctggacaa ttcgatcaag tagttggcga
    44221 ttactacgga aaaaaatatt ttggcgaagc tgctaaaaaa gacgtagaac aaatggtgaa
    44281 aaaaatgatc gccgtttata aaaaacgtct agaagaaaat gactggcttg gggatgcaac
    44341 aaaagaaaaa gccgtcataa aattaaataa cttaggtatc caagtaggct accccgataa
    44401 gatcccagca ttattcgacc agttcaaaac agtacctgca gatcagggtg gtactttgct
    44461 ttcaaatact cttgttttca gcaaactcac attaaaagat cgtttcagca aatggaacaa
    44521 accagtggat cgttcagaat gggaaatgag tgctgacacg gtcaatgctt attatcatcc
    44581 atttcggaat gtgatcgtct ttccggcagc gatccttcaa gcaccatttt acagtctaga
    44641 acaatcaagc tctgctaatt atggtggaat cggagctgtt atcgctcatg agatttccca
    44701 tgcatttgat aataacggcg ctttatttga cgaatatggc aacttgaaca actggtggac
    44761 agaagaagat cttacacaat ttcaaaacaa agcacaagca atgatcgaag aattcgatgg
    44821 tctgcctttt gctgacggaa aagtaaacgg aaaattaacc gtttcggaaa atattgctga
    44881 tgcaggcggt ctaagctgtg cattggaagc agcaaaaacg gaatcagatc tttctatcga
    44941 ggaattcttt gtcaattggg caacgatttg gcgtacaaaa gcaaaaaaag aataccaaca
    45001 acttttactg caaatcgatg tccatgcccc tgctaaactc cgagcaaacg tgcaaccaaa
    45061 aaaccttgac gaattctatg aaacatttgg tatcacggaa caagacgcta tgtatctagc
    45121 gccagaaaaa agagtgcgaa tctggtaaga ggttgtgcca gaagtggtca gcttcaagaa
    45181 ataagaagga atttgggaaa atcgcttctc aaatttttgt gacatttcgg cttatttttc
    45241 gaagaagtta cttctggaat accatgtatt agaggttgtg aaaaagctgc ttagtttcaa
    45301 gtagtaagaa aaattttgtg aaaatggctt tccacatttt tgcataaatt tgacttacta
    45361 ccgagaaact ggcttttgaa caccgtttat cgagaggttg tgaaatcagc gtcctgacct
    45421 gagtattaga gaaaaaaacg gaaaaatagt tcctcatatt ttgccgtttg ttgagctaat
    45481 accgagggtc agctgattga acaccgttta gtggagagat tgtgaaaaag ctgtcctgca
    45541 tcaagtaata agaacagcca atcaaaagca gcaaggagaa cctcatctat aaaaagatag
    45601 aaggaagtga gacatctatt ggtctcattt ccttctattt ttactttcct cagtcaattg
    45661 ttttacatca taaaaggaga aattccactt atttttttcg ctgtttcatg acataaacaa
    45721 ctacaccgat taacaaaatt cctccagcta atgctagcaa cgggttatta aattctcccg
    45781 tttttgggag tccttgagtg ccattttttt cactttcttt tgctgtccta tccggatgag
    45841 aaggttttcc atttggtttt ccatttggtg attcatttcc tggattttct ctttctggtt
    45901 tgtcatttcc cgggggcaca tcggcagttt ctcgcacagt gatcgctttt tgctgggtaa
    45961 cagattctgt atgagcagac catttttccc catcaaatat ttccgaacga taagtgactt
    46021 ctagcgtata ttctcctttt gaaggtgctg aaaaaatagc ggtatgatta gaaaattgag
    46081 cacttgactg tgtttcaacg atttggatct tttctgggaa ataacgcata tcttcaaaga
    46141 caggcgttcc ttgatttggt gtgaaagttg acacaaaaac tgtaaaaggc ttcagttcaa
    46201 tgatatttcc ttctgcattc tccgccacac catttgcttc ttgtataata atcggattat
    46261 tttgttcatt tggttttgtc tgatttggtt cttgtacgat tgcatactct ggatattccg
    46321 aagacggtga ggtttctccg tcaaaaatag cacctacaga taatggaaga ccatccactc
    46381 tttccttttc tgtttgacga ttccccggtt taatctgcat cagtccatga acatggatac
    46441 ttccattttc atagcgcgta acaggctgct gaccttcttt cgaacatgta tttagagact
    46501 cgatccaatc ttttgatatg gtatgtccaa gagtattttc tgattgtttc tctcgttcat
    46561 tccaatagta gttcaacgag tggtagattt gatcttcgtt ttgattcaat gggcggatct
    46621 gctctctcat ctcaagattc tctgttcgga aagataagat accagaagca tggtagtctg
    46681 tgtttcctgc tgaagaggta taaagagcca cattatatga accgttatta aaacttgtac
    46741 tgctattgac gataatatct gttccgctat tggaatcgat tcctttcgca agattttcga
    46801 aagacaaact attgatcaat tcatgagggc ccgttaaaga agaaccgccc attttgaatc
    46861 cattaccgtt accttctccc tcaattcctg gtacccagcc gtttcgataa gcaactgagt
    46921 ttttgatgat tacttttccg atcgaacctg tttcattttt agcaaaaagg tcccaaccgt
    46981 catctgcatt atgatacgcg atacaaccgt caaatacatt tccttctcct attgtcagtt
    47041 tggccgcaaa tccatcggca tcttcaaatc ctggatcggc attttcaaaa gatgtacagt
    47101 tcttgactaa gttatgcgcg ggccatgctt cgaaaggatc atttgagcta ccgctgattt
    47161 ggattcctgt atttccattc cgataagcat gaatctcttc gatcaaatta tgatttccag
    47221 aaatctgtaa tcctttttgc atagctgcac tatttgtaat gtcaaaccct cgtaaacccc
    47281 agtagtgact gaacagtgtc atcccatttc cagttccttt aaaatcaagt acaggacgct
    47341 tgttttctgg atttttctct gcaatcaact gaatcgggtt ttctcttgtc ccgttaacgc
    47401 cttttggaat cgtcaatcct ctttcgaagg aataagttcc aggcgccatg acgattgtct
    47461 gcccagcata tgcgtatctc aatgcttgtg tcagagataa tggattttgt ctgcttccgt
    47521 ttcctgcatc tgacccttct ggtgttacat aaactgtcga atatttaggt ttacggtagt
    47581 ccactgtatg aagaatctct tttgtttcat aactagtcat ctctacgtat tcttctggcg
    47641 gatagttttt atctggcgta aaactgaaag taaattcgtt cgttccttcc atcagttttg
    47701 tctgataatc aaattcccga ttcgcagtga tagccaagtt ttttgcgttc ttaagcacct
    47761 ctcctgttgc tttattttta attgtcaata atccgtcagc atttgctcta aaggacatag
    47821 tatgatcttg gtttcctgaa tgattactag aggtcacagt gtaaatcgga tcaatcaagc
    47881 gagtaggtct ttcttgccca ggaggatctg tcgcaggatc tgagtaagca acttcgattc
    47941 cttctacctt gatcgtcata ttccttgctg cagcaaatcc tgcataaata accgattcat
    48001 ctgaagcgta taatttttcc cagtcataca tgatgtcaga agccatttct tctcctgttt
    48061 caggatcgta ataatatgcc tctaaaccag tattcgtttt tttcatctct acgtccagtt
    48121 tagtaatcga gttcgtggcc ggaaacagat tcccatgacc gtttccgaat atattataag
    48181 aacctgtttc tttttccaag aaccctgctg aagtctctaa aggtgttgtt tccactgaga
    48241 cactcccagg agctggcgca gcttgcggat cttcagatga tattcctgtg attgcccgtg
    48301 tccccaatcc aagtcgcatc gtatatttgc tgccgctcgt gttgaccact tcttgtctat
    48361 ccgagtccca gacatattcg atccgactgc ctagtatggc aaatgaattt gaataaaatg
    48421 cctgcccaaa caaatgattt tttggtactg cgtctcttac cattaaagca aatccttctt
    48481 gggcattcgt ataggtccaa gattctattt cgatcgtcgc ccgcatccta aagttgatgt
    48541 ctttggggat tgcttgataa taataggtca ttccatctga tccgcttgac tggaattttc
    48601 cgccgttatt cgtggaagag agccgcacgc cgtcttctaa tggttcaatg gcatacctac
    48661 tagcacttgt gctttctccg aaaacaaccc ctttccactc ttcatttgct tcttcagatt
    48721 gcttttttgt cttttcattt tctgattctt tcatcatagg atcatttcct tcttccgtat
    48781 ccatatcttc actagttctt ggtatttcct ccattgttga catcccttca atgttcacgt
    48841 gtgcattttc ttcgttcaca gactgttgtt cgttagcagc aacttcaacg acagaaatta
    48901 aaaccggggt actgagcata aggagattca tcattagcgc actgtatttt atacgttttt
    48961 ttaacaagtg tattcctccc gtaacaaatt agaaagcgtt tacttaaagt attacctaat
    49021 ttatttttac tgtcaataaa aaacaaaatt ataat
//
LOCUS       DACDIG010000012        47471 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.012, whole genome shotgun sequence.
ACCESSION   DACDIG010000012 DACDIG010000000
VERSION     DACDIG010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.012"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(56..790)
                     /locus_tag="IV247_000759"
     CDS             complement(56..790)
                     /locus_tag="IV247_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP8776850.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1117..1527
                     /locus_tag="IV247_000760"
     CDS             1117..1527
                     /locus_tag="IV247_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAP8776851.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1520..2221
                     /locus_tag="IV247_000761"
     CDS             1520..2221
                     /locus_tag="IV247_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAP8776852.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2221..3807
                     /locus_tag="IV247_000762"
     CDS             2221..3807
                     /locus_tag="IV247_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8776853.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3826..4047
                     /locus_tag="IV247_000763"
     CDS             3826..4047
                     /locus_tag="IV247_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAP8776854.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4044..4805
                     /locus_tag="IV247_000764"
     CDS             4044..4805
                     /locus_tag="IV247_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAP8776855.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5185..5694
                     /locus_tag="IV247_000765"
     CDS             5185..5694
                     /locus_tag="IV247_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP8776856.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5769..6308)
                     /locus_tag="IV247_000766"
     CDS             complement(5769..6308)
                     /locus_tag="IV247_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAP8776857.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6448..7059)
                     /gene="rpsD"
                     /locus_tag="IV247_000767"
     CDS             complement(6448..7059)
                     /gene="rpsD"
                     /locus_tag="IV247_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAP8776858.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7375..8172
                     /locus_tag="IV247_000768"
     CDS             7375..8172
                     /locus_tag="IV247_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAP8776859.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8200..8835
                     /locus_tag="IV247_000769"
     CDS             8200..8835
                     /locus_tag="IV247_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAP8776860.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8855..9628
                     /locus_tag="IV247_000770"
     CDS             8855..9628
                     /locus_tag="IV247_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8776861.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10036..10833
                     /locus_tag="IV247_000771"
     CDS             10036..10833
                     /locus_tag="IV247_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAP8776862.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10811..11224
                     /locus_tag="IV247_000772"
     CDS             10811..11224
                     /locus_tag="IV247_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8776863.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11208..13853
                     /gene="mutS"
                     /locus_tag="IV247_000773"
     CDS             11208..13853
                     /gene="mutS"
                     /locus_tag="IV247_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP8776864.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13871..15979
                     /gene="mutL"
                     /locus_tag="IV247_000774"
     CDS             13871..15979
                     /gene="mutL"
                     /locus_tag="IV247_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAP8776865.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16001..16561
                     /locus_tag="IV247_000775"
     CDS             16001..16561
                     /locus_tag="IV247_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAP8776866.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16646..18634)
                     /gene="treP"
                     /locus_tag="IV247_000776"
     CDS             complement(16646..18634)
                     /gene="treP"
                     /locus_tag="IV247_000776"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAP8776867.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18857..19570
                     /gene="treR"
                     /locus_tag="IV247_000777"
     CDS             18857..19570
                     /gene="treR"
                     /locus_tag="IV247_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAP8776868.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19647..20093
                     /gene="msrB"
                     /locus_tag="IV247_000778"
     CDS             19647..20093
                     /gene="msrB"
                     /locus_tag="IV247_000778"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAP8776869.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20263..20865
                     /gene="ruvA"
                     /locus_tag="IV247_000779"
     CDS             20263..20865
                     /gene="ruvA"
                     /locus_tag="IV247_000779"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAP8776870.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20878..21879
                     /gene="ruvB"
                     /locus_tag="IV247_000780"
     CDS             20878..21879
                     /gene="ruvB"
                     /locus_tag="IV247_000780"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAP8776871.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21908..22486
                     /locus_tag="IV247_000781"
     CDS             21908..22486
                     /locus_tag="IV247_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP8776872.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22538..23020
                     /locus_tag="IV247_000782"
     CDS             22538..23020
                     /locus_tag="IV247_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAP8776873.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23137..23511
                     /locus_tag="IV247_000783"
     CDS             23137..23511
                     /locus_tag="IV247_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8776874.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(24189..25541)
                     /gene="brnQ"
                     /locus_tag="IV247_000784"
     CDS             complement(24189..25541)
                     /gene="brnQ"
                     /locus_tag="IV247_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP8776875.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
     gene            25688..26278
                     /locus_tag="IV247_000785"
     CDS             25688..26278
                     /locus_tag="IV247_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP8776876.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            complement(26406..27755)
                     /locus_tag="IV247_000786"
     CDS             complement(26406..27755)
                     /locus_tag="IV247_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8776877.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(27923..28663)
                     /locus_tag="IV247_000787"
     CDS             complement(27923..28663)
                     /locus_tag="IV247_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP8776878.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            complement(28676..30268)
                     /locus_tag="IV247_000788"
     CDS             complement(28676..30268)
                     /locus_tag="IV247_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8776879.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            complement(30836..31132)
                     /locus_tag="IV247_000789"
     CDS             complement(30836..31132)
                     /locus_tag="IV247_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8776880.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            31308..32033
                     /locus_tag="IV247_000790"
     CDS             31308..32033
                     /locus_tag="IV247_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAP8776881.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            32026..32868
                     /locus_tag="IV247_000791"
     CDS             32026..32868
                     /locus_tag="IV247_000791"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAP8776882.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELIALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            32871..33392
                     /locus_tag="IV247_000792"
     CDS             32871..33392
                     /locus_tag="IV247_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAP8776883.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(33645..34208)
                     /locus_tag="IV247_000793"
     CDS             complement(33645..34208)
                     /locus_tag="IV247_000793"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP8776884.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            34459..34728
                     /gene="rpsO"
                     /locus_tag="IV247_000794"
     CDS             34459..34728
                     /gene="rpsO"
                     /locus_tag="IV247_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAP8776885.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            34916..37042
                     /gene="pnp"
                     /locus_tag="IV247_000795"
     CDS             34916..37042
                     /gene="pnp"
                     /locus_tag="IV247_000795"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAP8776886.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            37552..37818
                     /locus_tag="IV247_000796"
     CDS             37552..37818
                     /locus_tag="IV247_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP8776887.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            37962..39953
                     /locus_tag="IV247_000797"
     CDS             37962..39953
                     /locus_tag="IV247_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAP8776888.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            40307..41608
                     /locus_tag="IV247_000798"
     CDS             40307..41608
                     /locus_tag="IV247_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP8776889.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(41738..43108)
                     /locus_tag="IV247_000799"
     CDS             complement(41738..43108)
                     /locus_tag="IV247_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAP8776890.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVNPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(43290..43613)
                     /locus_tag="IV247_000800"
     CDS             complement(43290..43613)
                     /locus_tag="IV247_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP8776891.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            complement(43938..44546)
                     /locus_tag="IV247_000801"
     CDS             complement(43938..44546)
                     /locus_tag="IV247_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAP8776892.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            44876..45592
                     /locus_tag="IV247_000802"
     CDS             44876..45592
                     /locus_tag="IV247_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAP8776893.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            45605..46300
                     /locus_tag="IV247_000803"
     CDS             45605..46300
                     /locus_tag="IV247_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAP8776894.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            46384..47013
                     /gene="udk"
                     /locus_tag="IV247_000804"
     CDS             46384..47013
                     /gene="udk"
                     /locus_tag="IV247_000804"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAP8776895.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
BASE COUNT        15392 a         8788 c         9878 g        13413 t
ORIGIN      
        1 aataaaaaaa cttggtacgc aatcgctgta ccaagttttt ttatttagct tttgattaag
       61 ccataaataa taatttatat aagaatactg ctacgacacc agcacagata ggtgctacaa
      121 ctggtaccca tgcatatccc cattgagaag aacctttatg tttcaatgga agaagtgcgt
      181 gtacaagacg tggagcaaag tcgcgggctg ggtttagtcc aggacctgta ggaccaccaa
      241 gtgaagcaac taatgtccat acaaggaaac caagtgctaa gtgagctgta ccaagattat
      301 cttggaagaa tggtgctttt gtaataccta gggcaccaaa gaataaaacg aatgaaccaa
      361 agaattcatt gacgaaacca ttgaatttag aaccaacgct gttgattgtt gagaatgtac
      421 ctaacacatg gtttacgtct tctgtttggt cgtagtaagg tttgtaacaa gctactacga
      481 tcaattgtcc aatcgctgca ccaatcaatt gtgcaatgat ataaggaaca acttcagccc
      541 aagggaatag accattagat gctaaagcaa ttgtaaatgc tgggttgatg tggttaccgc
      601 tgattccacc aaaaatcatt gctggcatca taaccccgaa tccatacccg acagcaatca
      661 gcatccagtc actgccatat ccttttgtac cttttaaatc tacgtttgct acagcaccgt
      721 tacctagaac gattagtaat gctgtaccaa tcatttccgc cgcaagacgg gtcattagtg
      781 agtaatccat aaatacctcc aaaagttata aaaatgcaaa aaaaaaagaa acatcaacac
      841 gaaaagcatc ggtgcagtgt ttcgtgtcgg aaggatttct gcagtcttaa caaatgtgaa
      901 taaaaaaatg aattccatta cttataagaa accagaccaa cagtaaaact ccaactcttt
      961 ttaaaagttc tccacaaatc accaagctta actataaata taccaaaaaa aaaacgtttt
     1021 ttttctcaaa aattgtatct tagaaacagc atgttttgaa actttcagaa aaggtttgtt
     1081 agtaaaatga aagctatacg taaggagtgt tgtttgatga aattttttca agagtttttc
     1141 attattattt tcttttctgt attaggagaa ggcatacgga tttttgctca tttacctatt
     1201 cctggaagta tcttaggtat tcttttttta tttttagcat ttgaaatgca tcttgttgat
     1261 cctgaaaaaa tcggagcaac tggtgatttt ttattgaata atctagccat tttatttgtt
     1321 cctgctggtg taggtttatt ggaatatttt gatgatattg cttcgatatg gcctgtttta
     1381 ctgggagcag tcgtcgtgtg ttccatcgtc acgatggttg cagcgggaaa aacggcagaa
     1441 ggtgtagaag ctttgctggg ctatgtccgt aagaaaaaag cagaaagggt atctgtaaac
     1501 gaaatggagg agaatcaaat tgactgagtt ttttgtgaat ccattatttg gcttaagttt
     1561 gactttagga gtgtacgtat tatctgacaa atttttgcgg aaatataaac tgcctgtttt
     1621 gaatcctctc gtattatcga ttgttgttat tatcttattt ttgcattttt ctgggatcag
     1681 ctataaagac tataatatcg ggggcaattt tttgactatg ctgattactc ctgcgacagt
     1741 ggcacttgcc atccctcttt ataaaaactt ccgtttactg aaagagaatt tttatcctgt
     1801 aatggctgcg attttagcag gaatagtcgc aaattgcttg gttagtgtag ggttcggtta
     1861 cttcttctct cttcataaag agatggtgat ctcattacta cctaaatctg taacgacggc
     1921 tatttctgtc gatttatctc atacgatggg cggggtgaat gccgtgacac ttgctattgt
     1981 tgtctctaca ggtatttttg gttcgctgat cgccactcat attttccgcc tattcaagat
     2041 cgaaagtcca gttgctcgcg gtgtggcgtt aggaagtact tctcatgcta ttggtacagc
     2101 aaaagcaatc gagattggtg aaatagaagg aatcatttcc ggacttgcga tatgcgtcaa
     2161 cggaatattg accgttttat tgctaccgtt attctttcag ccttttgctg gcttgttttg
     2221 atgcgaaaac cactatttta tgctacaata accaaaacgt tcgagcataa aaaaggaggg
     2281 aaagacgtgt atttgtcttc cgagtatctt aaacaatttg ataaagatct atattataag
     2341 attctcctcc tagaaagttt tgaggatcaa gcttggcata ccgctgcaca attagcgcaa
     2401 gttgttcagc tggatgcccg cagcgtgtcg aaatacctga acgagctgtc taaaaactat
     2461 cagcagttta gcggtaagac acaccctttg tttacaaaaa atcaccgttc tggttataac
     2521 ttttatgata cgctcgattc aattgaacat gagcgttttt tgatctatct cgtgcaaagt
     2581 acgctgaaat tccagttgct gcacgacatt ttttttgaag aattccatac aatgtaccaa
     2641 tttgcgcaaa agcattacat tagtgaatct actgctcatc gaaaaatcaa tgaatggaaa
     2701 cagcaactgc aaacttacgg aattcggctg caacgaggta catatatcgc tcaaggcgaa
     2761 gaagaaatca ttcgacttta cttgcatatg acattctggc agttgtttcg agggaaaatc
     2821 tggccatttg aaacgatttc tcaaatggac gtaaagaata tggcagaaca tatcatggct
     2881 ttttttaatg ttcgtctgaa cgagatcaaa aaaagacgat tggaatatat gctaggtgcc
     2941 ttttttttaa gaaaatcaca gaagcattat gttgtattga atgagaaaaa aagacggctg
     3001 atttctgaca atctgttgtt ccagcgattt tgccaggtca tggagccggt atttcctaat
     3061 tattttcaag tagaagacga gttaggcgca ttatttcttg tactgatgac tcgtgaagaa
     3121 tattatagtg atcctaagat acggaagaaa atttttgatt ttcatcaaca agcaaaaaca
     3181 ccgcccttta cagccctttc agaagcaaaa gccgctcttt ctctttatca ggaagagcag
     3241 gggctgccag cagaaaatct aaccttcgag gcagaaaact atttattcag cagtcatttt
     3301 ttcgcctatc tttttcctaa tgcgaaagaa acgattgatg gcaatagtag tgattttatt
     3361 aatcatttag ttattgaaaa caaggaattg aaacaatggc tcgtacattt ttttgaaagt
     3421 agacacaaac atcccaatca tctggcattt aaaaatcatg cgtttttgat ggggcggtat
     3481 ctgactgtgt ttaaaacatt aggagccttt actccacagc ttccaaagat cacgatcctt
     3541 ttaatgacag atttcccatt atttgaagag caactgttag aggagggatt acggacattt
     3601 ttccgtaatg agtatcagct gatttttctg ccaacagatt atcgggggag agaagttgat
     3661 cttctgattt cgacaagtaa agtccaccgc aaaccatggg cggatcttga ctattttatt
     3721 gtgactgaag aactaaaatt aattgattac atccaactat ctcaaaaatt tgaaatgatt
     3781 caaaaacaaa aactattaaa acaataacaa agaaggagaa aaaagatgac atttgaagaa
     3841 attttaccag aattgaaaaa aggtgcaaag attattcgta aaggatggag tggatttgaa
     3901 ctttatgttg ttctcgtatc cgatgatgaa tttgatggct gtccggtgac gccgtactta
     3961 ttgatcaaaa caagtgacga aggtttttcc agcttcgctc ctacagtttg tgatatttta
     4021 gcagatgatt gggcgatcgt agaatgaaaa accagtatcc agatctagct aaacaggttg
     4081 tatttatcac aggagctgct tctggaattg gtgctgcgca agcccgagcg tttcttgaac
     4141 aagatgcttt tgtatttgga ttagatcaaa agttcggtaa aatggaagaa atttctttgc
     4201 gatttcctga aacttttgcc tacgcactag gtgatgttcg acaaatgaaa gaactccaac
     4261 aagcggtatc gaaatgccaa aatcattttg gtgaagttac gattttactg aatacagcgg
     4321 ggatattgga tgcctacaaa cccttgatgg aaactgacga atcgctgtgg gatctgattt
     4381 atgagacgaa tgtgaaaagc atgtatcagc tgacaaaaat catccttcct gatatgatcc
     4441 gccaaaaaag cggaacgatc atcaatatgg cttcgattgc tggcttgatt gctggtggag
     4501 gtgggatcgc ttatacaagc gcgaaacatg cgattgtcgg atttaccaaa caactcgctc
     4561 tggattacgc aggacagggt atccgagtaa aaggaattgc tccaggagcc atccaaacac
     4621 caatgaatgc ggctgatttt gaagggaaag gtgagatggc agagtgggtg gcaaatgaaa
     4681 caccagtcaa gcgatgggca cagccggaag aagtagctga gctgacgtta tttcttgcct
     4741 ctcctcaagc aagctacata caaggaacga tcatcccaat cgacggcgga tggttgttaa
     4801 aataaccttg tgtttttttc aagcttctgt atgataaaac taaagagaga aacaacggtt
     4861 gatgataaaa aaagtcgatg actgttcccg acattcgatg ccgaacagcc agttcaaaaa
     4921 attcttttta ataatcgtta atattcgaaa ctaagcggct tttcacgacc tcttgatata
     4981 aacgatattc aaaagcttgc aactgcggta acaagtccag ccaaatgaaa aatataagga
     5041 gctattttta tttgaccatt cttattactt gttgcagggc agctttttca ctaccttctg
     5101 cgacacttca gttggaattt tcttcccatc ctttggtcgc ggtcattacc aagataaaat
     5161 tatcttgcaa aggagaaata gaacatggaa aaaaataata cccgttcagc caaacgctgg
     5221 acaacgacag atgtgactcg gatggcgcta gtcactggtt tatatgtggc agtgacgctg
     5281 gttttgtctg tctttagctt tggagtgatt cagattcgtc tctcggagat gttcaactat
     5341 ctgtccttgt ataacaaaag atacatcgtc gcggtaacag cgggagtagc tttggctaat
     5401 attgcttctc ctcttggact gatagatgtt gcagtaggaa gcgtcagcac gctgcttgta
     5461 ttgcttatca actataaaat cactagtcgc atcaaaaaca tgaaaatcaa aatggccgtg
     5521 acagcatgcg tatttgcttt ttccatgttt acagtagctg gtcagctaac gattttatat
     5581 caactacctt ttttcttgaa ttggtgggtg attgctttag gagaattggc atcaatgatt
     5641 gtcggtggga taattattta ttggatcggt aaaaaagtgg atttgtcaaa ataaagatct
     5701 gtttaattta aaatagggca gcatggaaga gactgaatgt tgagtcttct tccatgctgc
     5761 ttttttgatt aatttgtatg aagaaatcga tttagccgtt ttttcatcaa gataccgata
     5821 taagctgctc cagttgcctt gattgcttcc ggcagtaaaa acggcagtag tccactggca
     5881 aatgcagttg taaaagtaag atcaccactt attttcagcc agatcgttcc gcagatcaat
     5941 gcagcaagag agcccattag attggcaaag attgcatata agtaatgagc atccgttctt
     6001 tcgatcatcc aaccagttat ccacgtttgt aagagaaatc caatcaaata tccgccagtt
     6061 ggcccaaata aaacagctat acctccagtc atccctgaaa atacaggaag accaatcaag
     6121 ccaagtaaaa gataaactcc aacagatatc aatcctagtt ttcttccgag aaaagttgct
     6181 gttaatccaa tagcaaatgt ttgccctgtc aaaggaacca aaccaaaagg aatggtcatc
     6241 tgagaaagga cagaaataat cgcggcaaat tccgcagcaa gtatcaattc tttcaatgtt
     6301 cggttcataa cgccctcttt tcgttaactt aatattttta tttggttaac gaaattgtaa
     6361 aacaaagtga ttactttgta aatagaaaat ataaaaaaag accagctatc caatagatag
     6421 cggtcttttt caagaaagct tttgaaatta taagctttgg ttgtagtatt caacgacaag
     6481 agcttcgtcg atttctggat atagttcgtc acgttctggt aaacgagtca atgaaccttc
     6541 caatttttca gcgtcaaagc ttacgaatgc tgggcgtcca acagttgctt ctacagcttc
     6601 ttttactgtt acgatgtttt gtgatttttc acgaactgag atcacttgac ccacttctac
     6661 gtggtatgaa gggatatcaa cgcgtttgcc atcaactaaa atgtgaccat ggttaactaa
     6721 ttgacgtgct tgacgacgag tcgttgcaag tcctaaacgg taaacaacat tgtctaaacg
     6781 ttgttctagt aagatcatga agttaacacc atgtttacct tctttgattt tgctagcttt
     6841 tacgaacaag ttacggaatt gacgttcgtt cattccgtac atgtgacgaa gtttttgttt
     6901 ttcagttaat tgcatgccgt attctgattg tttgccacgg ctattaggtc catgttgacc
     6961 tggtgcgtaa gggcgacgtg ctaattcttt tcctgtgcct gacaatgaga tgcctaagcg
     7021 acgagaaatt ttccatgatg gtcctgtata acgagacatt aaaatttcct ccaatgaata
     7081 taagtttttg gagtaaaata atccgttgaa aaattcataa tcatatagtt tgttcttcaa
     7141 tcttcacctt tgcagccgcg gttacacaat cgaacccgag acggggcaac aaactaggga
     7201 tgagttatta tttttctgct gcattatttt acacaaatta cagtatatga tagatcatcc
     7261 acgaatgtca agataattag tacataaaaa cgctttacaa acctaggctg tctttataaa
     7321 atgagagggt atgtgcaaat ggaaaaaagc gaaggaagtt taaggagaaa atcaatgaat
     7381 ccggtatcac cattatttga gaaaatcgat gcttcaattg aaaagaaggc gaatcttttt
     7441 gaaaacagct tttcacgtta cgctgttcga gcggttctcg cctgtttgtt tttgacttta
     7501 ggaacggctg tggcatttgc gatcgcgatg aagggggaag atatcgcgca tggtctagga
     7561 aagattttat acgcatttat gttcagctgg ggtctggtca tgattctata catgaacgcc
     7621 gaattaggaa catcgaatat gctttatatg accgtgggtg tttttcggca gagacttgct
     7681 ttaagtaagg cagccaagat tctatttgcc tgtatttttt tcaatatgat tggcggtata
     7741 ttatgtggat ttctaatttc tatgactacg ccatttcaag atttaacgtt ggacaacttt
     7801 atgttagaat caatagcagt aaaactagca aaaccttcag caacgatttt ggtggaaggg
     7861 atgtttgcga atatcgttgt caataccgct gtactgatca gcatgcggat gaaagaggat
     7921 gtcggaaaag tgatcacagt cgtattcatc attttcattt ttgctttttt aggatatgaa
     7981 cacgtgatag cgaattttcc tgcctttacg cttacttact ttgcttctca cggacagatg
     8041 gatggaatga cagtcagcaa tgtgttacat aatttgtttt ttgctttggc aggaaattat
     8101 attggtggcg gtttagtaat gggactggga tatgcttggc tgaatcagtc aaaatcatcc
     8161 tatgtggact aaaaagtcgt tcattaaagg ggaagtgcca tggaagtttt agtttttcca
     8221 aaaaatgagg aaaaagcgtt acaaatggaa aagtacatga aaaatctttt ttcctttgct
     8281 ggagtaccta agccggaacg cgctctttta caacaaccgt tactggatga gagcaaaaat
     8341 ttatctattt ctgaagtttt agcttttgtt cattattatt atgaacaaat cgaaagagag
     8401 tatcagtatg tagcgatcga tttagtccag aaaaacatca aacggcttac atttgaagac
     8461 ttagtcgtat tgttgccact agttcagaaa aaagaatggt gggacagcat cgactcttgg
     8521 agaaaagtat acagcacatg gtgtaaagcc tacccagagc agcgtgaaga agtctttcaa
     8581 ttgtttgaaa aaaaggaaga tttttggcta cgtcgaatag caatcacgtt gcaattaggt
     8641 tataaacagc aaacagacac aggactattg atccgagcaa tagacgaaga tcgtcaaaca
     8701 aaggaattct ttatccaaaa agcaattggt tgggcattac gcgattatag taagaccgat
     8761 gccgattggg taaaaagaca gctgacaaca gatttgagta agttagctgt tcgagaggca
     8821 agtaagtatt tataagaaag cgagacgaca agagatgaga cgcaaattat ttgcttttga
     8881 tattgatgga acgttgttag attcaaacaa acaagcttta gatagcacaa gagaagcatt
     8941 ggaaaaatta cgcaaagccg gtcatctggt cacgattgct acagggcgga gccgatttca
     9001 tgcgcaagag atcattcgcg atcttgattt tacgaactac attttatgta atggagcggc
     9061 cggtttttta gaccatgaac aagtatataa gaatttacta gatcgcgacc agttggatgc
     9121 gttcgttcaa gaagcgcatc atgagcagat cgatacagct tttgtcagct tagacaatat
     9181 caagcgttct tcttccaatg atgtaacggt gatggaagaa gcgatgcatt ctttcggtgc
     9241 tgtcttgcct gaattagatc agttttttgc agaggaacaa gaaatatacc aagcgttagc
     9301 atttttcgat caaacatatg atgggaagtt ctcttcatat gataaaattc gatttgtccg
     9361 ctggcatgag aacagtgtag atgtcgtacc taaaaatggt tctaaagcag cgacaattct
     9421 gcacttagca gaacaagtag gcatccctca tgaagacatc atcagctttg gcgatggaca
     9481 aaacgaccgt gaaatgttgc gcatgtcagg aattgggata gctatgggga atgctgtgcc
     9541 agaagtacaa gcagaagcta agatggtcac tgatacaaac gatcaggatg gaatttggaa
     9601 ggcattgaaa gagttaagcg tgatttaaaa gaggttgtga aaaagctgtt ctgcatcaag
     9661 taataagccc agccaaacaa aaatagctcc tcctattttt cgtttttctg ggcttattac
     9721 cgcagatgca agctttcgaa caccgtttaa ggaagaggtc gtgacagaag tggtcagttt
     9781 caagaaataa gaagggattt acgaaaattg cttctcaaat ttttgtaaaa ttacggctta
     9841 tttctgaaga agtagatgaa acaacgttta ttcgggaaat gggactgtga caagatgttt
     9901 gtcatagtcc cattttttta ttttattttt caaaactaag aaataccagg aaagagttcc
     9961 ttttgtttga ccttttcttt cttattgaaa acaactataa tatagagtga tgttaaaaga
    10021 gaagaggaat ttagtgtgcg ggtattgttt attggtgatg tagtaggttc aatgggacga
    10081 gagatgatta ctgaatatct tcctagattg aagaagaagt accgtccgca agtaacgatt
    10141 gtaaatggtg agaatgccgc ttctggacgt ggaatcacag aaaaaatcta taaaaaattt
    10201 ttgcaagacg gcgtagatgt cgtgacgatg gggaatcata cgtgggacaa ccgcggaatc
    10261 tttgagtttg taaatgaagc taaaaagatg gttcgtccgg caaattttcc ggaagggacg
    10321 cctggacaag ggatggtctt tgtcaaagtg aaccaagtcg aactggcagt gatcaacatg
    10381 caggctcgtt cttttatggt ggacttggat gatccttttc gtaaaatgaa tgagctggta
    10441 gaagaagcca gaaaacgtac gccgatcatt tttgtagatt ttcatggaga aacaacaagt
    10501 gaaaaacaag caatgggctg gtttttagat gggaaagttt cagcagtggt cggtacacat
    10561 acacacgttc agacaaatga tgcgcgaatt ttgccaagag gaacagcgta tctgacagac
    10621 gttgggatga caggtcccta tgatggtatt ttgggtatgc gcagagaacc tgtaatcgaa
    10681 aagtttctga ctgcattacc aaaacgtttt gaagttgtgg aacaaggccg atcgatttta
    10741 agcggctgta tcttggaatt ggatgatacg acaggtcatg caaaagaaat ccagctgatt
    10801 cagatcaacg atgatcgacc gttcatggag tagaaagaga ggcagttttt tgcagcagga
    10861 gccggaaatt caaaaagaag taaggaaact gacaaaactt ttaagagaga atgaaacgat
    10921 cattcgctac aaagaacttg aagaaaagat ccagcaaaac cagtatttag ctgagttgag
    10981 agaaaaaatc aaacaagcgc aaaaagatgc tgttcatttt gcccactatg acaaaccagc
    11041 agcagaaaaa gaagcaatca aacaagcgga tcaattcatg caagaatttg atcagcatcc
    11101 gcttgttgta gcttatagaa aacagctgtt ggaagcagat gatctgcttc atcatttaac
    11161 aactatgata caagaagaaa tcaatggaca aatagaggag gaaaaacatg cctcaaaaaa
    11221 ctaaaaatac tcctatgatg gaacaatatc taagcatcaa ggcccaatac caagatgctt
    11281 ttttgttcta tcgtttagga gacttttatg aactatttta cgaagatgcg atcaaagcgt
    11341 cacagctttt ggaattaacg ttgacgagca gaaatcgaaa tgcagaagaa cctattccta
    11401 tgtgcggtgt acctcaccat gcagcacaag gatatatcga tgccttgatt gaaaaaggct
    11461 ataaagtagc gatttgcgag caggtagaag atccaaagac gactaaaggg atggtcaagc
    11521 gcgaagtgat ccagctagtc actcctggta cagtcatgga cagcaaagga ttgtcggcaa
    11581 aagacaacaa ttttttaaca gctgtagtta gtcaaggaaa tgaatttggc tttgcttatg
    11641 ccgatttaag tacgggagaa ttgaagacgg ctcggttgca tgatgaagaa gctgttttaa
    11701 atgaagcctc tgcattgcag acaaaagaac tcgtattagg cagtgagatc actgataccc
    11761 ttcgtgaaca gctaagcggg cgtttagggc tagtcttttc tcaacaaaat gagatggaag
    11821 aaaacgcgga atttagcttt ttgacaagcg aactagtcga tccgctggaa aaagaagcaa
    11881 ccggcaaact attgacttat ttatcggtga cacaaaagcg gagtttagct catatccaaa
    11941 aagcagtaga ataccaacca gatcattttt tgaaaatgga ttattattca aaattcaacc
    12001 tagaattaag ccaatctatt cggactggga aaaagcacgg cactcttcta tggttgttag
    12061 atgaaacaaa aacagcaatg ggtggccgtt tactaaaaca atggctagac cgtccgctta
    12121 tccaggcaaa acaaattaaa gcaagacaag aaatggtcgc ttctttgcta gacagctatt
    12181 ttgaacgtat cgatttgcag tcagcattaa cgaaagtcta tgatctggaa cgcctagcag
    12241 gtcgagtagc tttcggtaat gtcaatgggc gagacttgat ccagctgaaa acttctttag
    12301 aacaagtgcc aatgatcaga gggttgattg aaggaatcga tcaaggtcaa tggcaaagtc
    12361 tgctttcaga aatgcagccg atggaccatc tcgtccaatt gatcgaccaa gctatccaag
    12421 atgatccgcc gttacaaata acggaaggaa acatcatcaa agatggattc aatgagcagc
    12481 tcgatatgta ccgttctgct atgagaaacg gaaagaaatg gttggcagaa cttgaagcaa
    12541 aagagcgtca agaaacagga atcaagaact taaaaatcgg atttaaccgc gttttcggtt
    12601 attatataga aattacaaaa gcaaatcttg gaaatgcaga actggagaag tatgaacgaa
    12661 aacaaacttt agcaaatgca gagcgtttca tcacaccaga actaaaagaa ttggagacac
    12721 aaatcctcga agcagaagaa aaatctgttg atttagaata tcagttgttt ttagctgtga
    12781 gagaagaagt caaaaaggct atccagccgc tacaagtact tgcaaaagca atcagtgcag
    12841 tagatgtgct acaaagtttt gcaacgatca gcgaacgcta tcagtatgtt cggccagaac
    12901 ttgtcagtga caagcatcag cttctgatca aagacggacg acatcctgta gtagaaaaag
    12961 tattaggaca tcaggaatat atccctaaca gcgtagaaat ggcagaagac gaaatgatct
    13021 tgctgattac aggaccgaat atgtctggta aaagtacgta tatgcgacag ttggcattaa
    13081 ctgttctgat ggcgcaaatg ggctgtttcg ttccggcaaa acaagcagtt ttacctattt
    13141 ttgatcggat ttttacccga attggtgcaa gtgacgactt aattgctgga caaagtactt
    13201 ttatggtaga gatgatggaa gccaatcaag ccctgcgtta tgctacgcca aacagtttga
    13261 ttttgtttga cgaactaggg cgaggaacag caacctatga cggaatggcg ttggcccaag
    13321 caatcatcga atatatccat cgtcatgtac aagcaaaaac actcttttct actcattatc
    13381 atgaactgac ggtacttgag aaagaacttc cgcagcttaa aaatgtccat gttggagccg
    13441 ttgaaaaaga cggagaagtt gtcttcttgc ataaaatgat ggatggacct gcggataaaa
    13501 gctacggcat acatgtggca aagatcgcag gattgccttc caatctacta gaacgagcag
    13561 cagatatcct gcatacgcta gaaaatggcg ataacggaca ccatactgtt ccagctgaag
    13621 tagaagagaa aactaaaaca gccaaacagg agcaaataaa accacctgca gaaaaagtaa
    13681 atcaagacca tatacaagac cgtgtaaaag aagaaaacca acagctttct ttattcagtg
    13741 aattatccga aaatgaaaca gaagtcattg aatcactgaa gcaaataaat ttactagaaa
    13801 tgacgccaat ggatgcacta aacacattat atgaattaca aaaacaactg taggaagaag
    13861 gcggatatcg atggccaaaa tccaagaatt atctgaacgt ttagctaatc agatcgctgc
    13921 gggagaagta gtggagcgac cagcttccgt tgtgaaagaa ttagtggaaa atgcaattga
    13981 cgccggcagt actcagattg atatcttagt tgaagaagcg ggactgcgga cgatccaagt
    14041 gattgataac ggagaaggca ttttggcaga cgacgtagag aatgccttca agcggcatgc
    14101 gacaagtaag atccataatc gcgacgatct ttttcgcata cgaacgctgg gctttcgagg
    14161 ggaagctttg ccaagtattg cctctgtttc tgaaatgact ctggaaacgg cgaatcagga
    14221 agaaaaacaa ggcacctatc tgtcgctgaa aggcggagaa gttattgagc atcgaccagc
    14281 accactgcgt caaggaacga agattaccgt cagcaatctg tttttcaata cgccagcccg
    14341 cttgaaatac gtgaaaacac tgcacacaga attagcgaac atcggggaca tcgtcaatcg
    14401 actggcattg agtcatccat cgattgcttt tcgtttagta catgatggaa acaaaatgct
    14461 ggcaactgct ggaaatggcg atctgaaaca aacaattgcg ggaatctacg gattatctac
    14521 cgcaaaaaaa atgctgaaaa tcgaaggaaa agatctagat tttgagttat ttggctatgt
    14581 ttcattgcct gaggtgactc gtgccagtag gaattactta tcgacgatca tcaatggacg
    14641 ttttatcaag aacttttcct tgaataaagc aatcgttgca ggttatgggt caaaattaat
    14701 ggtcggtcgt ttccctattg ctgtattgga aatcaaaatg gacccgcttt tagtagatgt
    14761 aaatgttcat ccgacgaagc aggaagtccg gctgtcgaag gaaaaagaac taacagagct
    14821 aattagtaac gccattcaaa aagcattgtc tcaagaaaat ctgattccaa gtgcagcgga
    14881 taatctacgg tttaagaaaa aagtacctga tcagcctaaa acagaacaaa tcgaaatcga
    14941 tttgtcctac gcttttgaag aagaaaaacc taaaaagcca agtagtttga attttgatcg
    15001 agagacaggt acctttttcg tagaagaact aaagaacaca tttgttgaca gaattgttga
    15061 taagtctgtg gaaaatgttg aaaactattc tggtttttct gaagaaaaca ccacttatcc
    15121 agaaaaaaca gcgaaaacgg tggataagtt tggtgaaaat gttgataact ttgtagaaaa
    15181 aacagtggaa aaatctgaaa agaaagaatt tcccattgtg gataacatgg agaaaacaaa
    15241 tgaagaacaa gcagataaat ccgtagaaga ggatgaaaag atttacgagg aagaattgac
    15301 actccatcct gaatttgatt tcactcaagg aaacgacaaa acattagaac aagccatcaa
    15361 gaaattagaa acggaaaaac cagtacaacg ttttcctcag ttggaatatt ttggacagat
    15421 gcacggaacg tatctttttg cccaaagtag cgatgggttg tacatcattg atcagcatgc
    15481 tgcccaagag cgcattaagt atgaatattt ccgggaaaaa attggggaag tatctgatga
    15541 tctgcaagaa ttgcttgttc catttgtgtt agactatcca aacagtgatg ctttgaaatt
    15601 aaaagagcaa aaacacttgc tagaagatgc agggatttat ttagaagaat ttggtcaaaa
    15661 cagctttatt gtacgggccc atcctacttg gtatcccaat ggagaagaag aatcgattat
    15721 tcgggagatg atcgatatgc tgctgacaac tggaagtgtg agtgtaaaaa aattccgtga
    15781 agcaacagca atcatgatga gttgcaaacg atcgattaaa gccaaccatt atttgaatga
    15841 agcacaagca cgtgtgctgc tgaaggatct ggcacgatgc gaaaatcctt tcaattgtcc
    15901 ccatggccgt ccagtattga ttcactttac caattcggat atggaaagga tgttcaaacg
    15961 aatccaagac ccgcattaag tcaaagagaa gagggaaaac atgaagattg tattagcatc
    16021 ccaatcacca agaagaaaag agttattggc tcatatcgtt tcagaattcg agattgaacc
    16081 tgctgatatc gatgaaacgc catttccaga cgaacagccc gtcgattatg ttcgtagaat
    16141 ggctgaagaa aaagcacgaa ctgtctggga acaagatgaa aaagaagacc atttagtgat
    16201 tgccagtgac acgactgttg ttctcaatca agagatcatg ggtaaaccag aagatctggc
    16261 tgaggctgaa ttcatgctga aaaaactaag cggggaaaca catactgtat atacagctgt
    16321 tgttttaaaa acaaaggaac gagaagaacg gatactagca gaagcacatg tcacttttta
    16381 tcctttgaca gacgaagaga tcaaacgcta cttggagacg ggggattatg cggataaggc
    16441 aggggcatac ggcatccaaa atgctgcgag tgtattcgtc aaagaaattt ctggagatta
    16501 ttacagtatc gtaggatttc cgattggtgc tgtcaatcag gcactgaaaa cattcattta
    16561 aacaaaaaag cggactatct gattttcgat tgaaaatcca tgatagcccg ttttttttat
    16621 tgaaaagatt acttatctac tctgactaag attttacttc agctttaccg actgcttcgc
    16681 ccgggcgaac aagtcctgat tggtcaagag aaagtcctgc aactttttca ttattggtca
    16741 aagcaacaat aatgtcagtt ttcttgccag cttgttctat ttctggcaaa tccatttcag
    16801 caatcagggt ctctggtgtg acaaggtatc cttctttcac gaatacattg aaaggaacac
    16861 cctttagttc aacagtatct aatcccatgt gaacaagtac ttccagcccc tcattagaaa
    16921 gaataccaat ggcatgtttt gtttcaaaga cactcgtcac tttaccgttt actggtgcat
    16981 agactttgcc gtttgacggt tcgacagcat aaccgtcacc catcattttt tgtgagaaaa
    17041 ctgggtcatt tacttcagtt atttccttga tggtccctgc agcgactgcg aataatgttt
    17101 cttttgttgc aactgccgta ccagttgctg aaacaggttc gaaagactgt ggcgttgctg
    17161 ttgcgccatt tgctgtttga agttgtacat cagcagcatt ttcaggaaca gcaggatcaa
    17221 tcttattcaa aattccctga cgtctaaaga tgatcgtcaa gccaaatgga atgatgacag
    17281 caatgatcat tgcgatgatg aaaggtaccc atgtttctgc ttggatagct agtattcctg
    17341 gcaaaccgcc tactccgatc gcgttagctc gaacacccat caagttagca aacatgccag
    17401 ctaatccaga gccgaccatt gcagcgacga aaggatagac gtatttcaaa ttgattccga
    17461 acatagcagg ttcagttacg cctaagtaac aagaaatcat tgctggaatc gagatttgtt
    17521 cttctttttt attcccacga tgtaagaaga tgatggctaa gactgcagag ccttgggcga
    17581 tatttgataa agcaatcatc ggccataaat tcgttgatcc gaaatctgca atcagctgca
    17641 tatcgatggc attggtcata tggtggagac cagtaatcac taaaggagca tacaggaatc
    17701 cgaatacggc actaaacaac cagctgatag aagacgtcaa tccagtattt acgatatttg
    17761 agatccagct tccgactgtc cagccgatag gacctaaaat aacgtgtgca gctaaaacag
    17821 ttggtaacaa tgaaaacaat ggaacaaaaa tcatcgagat cgactgaggg atatatttgc
    17881 ggaagaagat ttctagatac gctaacataa accctgccag catggctgga ataacttgcg
    17941 cttgataacc aatcatctgg acttgagcaa aaccaaaatc ccaaaatgga atatctgctg
    18001 cggctgtgga agctacacta taagcattta ataattgcgg agaaactaac gtaattccta
    18061 aaacgattcc taatatttgt gtagtaccca tcttttttgc aatactccaa gtgataccta
    18121 ctggaagaaa atggaaaatt gcttcaccgg gtaaccacaa aaaggcattg acaccattcc
    18181 aaaacttcga gtgttcaacg attgttccac caagactttc aaactggata ccttcaagta
    18241 catttctgaa tccaaggatc aatccaccga caatgattgc aggaatcagt ggtgtaaaga
    18301 tttctgctaa aacggcaata gcacgttgaa cgggatgaag gttttgtttt gcagctgctt
    18361 ttccttgttc tttggaaacc ccttcaactc cagatactgc aacgaagtcg ttataaaaag
    18421 tagaaacatc gtttcctatg atgacttgga actgtcctgc attcgtgaac atccctttta
    18481 cgctaggtat atcttcgatc gctttttcgt ccgcttttcc tggatcattc aggacaaaac
    18541 gcatacgagt cgcacaatga ctaactgcag caatattttc ttttccgccg atatcatgta
    18601 acagcttctc ggcatctgct tgatattttg ccatatcaat aaaccccttt ctcttaactt
    18661 gtctatgcaa gttgtttctt catttactat actttttttc ggagagaatt gcaagcgttt
    18721 tattaaaaag tacgagagaa aatgatatcg ttttaaaaag cttaaaataa gcttttctta
    18781 aaaataggaa cattgaaata caaaaagagc tgtagtatag tgaagaaatg atggaagaac
    18841 gagtgaggga aaaaacatga ataagttcaa tgaaattttt ttagatttgg aacagaaaat
    18901 tttagcgaaa gaatatcctc cgcattcgct tttacccagt gagaaccaat tgatcaaaat
    18961 ttatggtgtt tcacgagaaa caattagaaa agcgctgaat cttttgacta cggaaggtta
    19021 tattcaaaaa aaacaaggaa aaggttcgat cgtactggat cggaatcgct tcgactttcc
    19081 tatttctgga ttgactagct ataaagagct acaggagacc caacgtattc caagtgaaac
    19141 gatcatccat acgttagaag aaacagaagt gacaaaagca ttgaacgaga tcactggctg
    19201 ggagatcggt gcgcctgttt ggcatttgat acgcgaaaga aaaatcaatg gcgaagtagt
    19261 tatattagac accgattatc tattaaagga aatcgttcct attttaactc cagcacaagc
    19321 acaaggatcg atctatgaat attttgaaaa tgagctttct ttgacgattg cttacgcgca
    19381 aaaggagatc acagtagaag aagtaacgga aatcatgaaa acagcgatgg atcttcacga
    19441 tgacacttat gcagtagtag tcagaagcct cgttcatctg gaagataccc gctgtttcga
    19501 gtattctgag tcggttcacc gattagataa attccgtttc gttgagtttg cccgacgccg
    19561 aaaagtgtaa atagttcaaa agaaagcaaa ttgttggcat ctgtccactt tggcgctatg
    19621 ataggatcac aaagggggca tttttaatgg ataaaccaat aagaccagaa aaagaagaac
    19681 tgaaagaaaa gttatcaccc atggcttatg cagtcacaca agaaaatgca acagaacgac
    19741 catttagcgg gaaatatgat gatttttacg aaaaggggat ctatgtagat atcgtcagcg
    19801 gtgaaccgtt attcagctct gcagaaaaat atgatgcagg ttgtggctgg ccatcattca
    19861 gcaaaccaat aacgaaaaga caagtgcgcg aaaaagccga tttttctcat ggcatgcatc
    19921 gaatagaagt tcgaagcaaa gaagcagatt ctcatctcgg acatgtgttt accgatggac
    19981 cagtagatca aggtggtcta cgttactgca tcaattctgc ctcactgaaa ttcattccat
    20041 atgaaaagat ggatgaatta ggttatgggg aattcaaatc acttgtagaa taatggaaat
    20101 acacaaagag cggtacaata cggggattta ctcgatcgtc cgctcttttt ttattcaagc
    20161 gattgctatt tgaaagaaac gctccctaga ctcctttttt cacaaggtga aatgtgctac
    20221 aataaaggga acgtatgtgt gcaaaagaaa gaggactaga atatgtatga atatctgaca
    20281 ggtgtggtta cctttatcaa cccctattac atagtagtag aaaccaatgg tatcggctac
    20341 cagatcgcac taggaaatcc ttaccgctac agtagcaaac taaatcaaaa aataaaaatt
    20401 tatgtccatc aggtaatacg tgaagatgcg catcttctgt atggatttga ttcgctagaa
    20461 gaaaagcaac tgtttttgcg attagtaagt gtttcaggaa tcggtccgaa aagtgccctt
    20521 gcaatcatgg caagcgaaga ccactccggt ttgattcaag cagtagaatc cagtgatgtc
    20581 acttatttga cgaaatttcc aggagtaggg aaaaagacag cacaacaaat gatcttggat
    20641 ctaaaaggaa aattcggtga gctgagtatc gatacacctt ttagtctatt tgatgaagca
    20701 aacacaaaag acgctacagc tctatctgaa gcaatggaag ctctctcggc tcttggttac
    20761 agtgatcgag aaatcaaacg tgtagaaaaa caattaaaag aagtagataa tcagacaacg
    20821 gatgagtatc tacgccaagc actcaagcta atgatgaaaa aataaggagg agaccaaatg
    20881 acagatgaat acttgttatc accagaatct ggcgaaaatg aactgagttt ggaaaaatct
    20941 cttcgaccac agctgctttc tcaatatatc ggacagagaa aagtcaaaca agaacttcag
    21001 atttacatcg aagctgccag aaatcgggaa gaacctttag atcatgttct gttgtacggg
    21061 cctcctggat taggaaaaac gacgatggcc atggtcattg ctaatgagat gaccgtaaat
    21121 attcgtacga cgagtggccc tgctatcgaa cgtccgggtg acttagtagc aatcttgaat
    21181 gaactggaag caggggatgt cctgtttatc gatgagatcc atcgattacc tagagtggta
    21241 gaagagatgc tttattcagc tatggaagat ttctatgtag atatcatggt aggacaaggg
    21301 ccaaccgcac atccggttca tttccctctt cctcccttta ctttgattgg cgcgacgaca
    21361 cgggcaggta tgctttctgc acctttacga gatcgatttg gcatcatttc tcatatggaa
    21421 tattatgacg aacaggatct gcaggagatc gttttacgtt cttcggatat ttttcaaaca
    21481 gagatcattg aagaaggggc attggaaatt gctcgccgat cacgaggaac gcctagaatc
    21541 gctaatcgct tgctcaaacg ggttcgtgat tttgcgcaag tccaaggaga cgggaaaatc
    21601 gatcgtccta tagcggatca ggcacttact ttgctacagg tcgatcaggc tggattggat
    21661 tatgttgacc agaaattgtt gcgaacaatg atcgagttat atggcggcgg accagtaggg
    21721 ttaagtacgc tttcagtaaa cataggagaa gagcgtgaga cggtagaaga tatgtatgaa
    21781 ccgtatctga tccaaaaagg atttttaaag cgtacaccta gagggcgaat tgctactgct
    21841 tatgcctatg aacattttgg ttacgactat catatatgat ttttgctttt agattggagg
    21901 aaaatgaatg agtgatacaa cacgtacgaa acaattgatc aaaaacgtat tgattgattt
    21961 gtgccaaacg caatcttttc gaaaaatcag cgtccaaaaa atcgcaaacg aagcaaaaat
    22021 caatcgacaa acattttatt atcattttac agataaatac gatttgcttc gttgggtcta
    22081 ttgtgaggat gcattgcatt atttagatcc acaatgtctt tcacttgata attgggaaga
    22141 gcaagcattg aaaatgctga aagcaattgc tgagaagaaa gaattttatg caaatactgt
    22201 gctttctgac ccagaaattt tacaaaaaga gttttctcaa ctgatccaaa aatcatttat
    22261 caagattttt gaacaaatgg atgaagaaag acaactttct gcaagagaca aactatttta
    22321 tgctcgtttt ttttcttacg gttgcagtgg tgtattagtc aattggatca cgaatgaata
    22381 tgaagaaaca ccgatggaaa tcgctatgca gcttttccgt ttagccaagg atacggaatt
    22441 atattcttat cgtctttatg caaaagaaga gttggaagaa cagtaataga aaaatttttt
    22501 gttggaaaag caataatcgc taaaaggaga tggggaaatg acaaaaattt tatttgtttg
    22561 tttgggaaat atttgccgtt caccgatggc agaaggcttg ctgagaaaac gaatagcatt
    22621 agaaggaagg gaagcagaat tttttgtgga ttctgctgct acaagcacgt atgaagttgg
    22681 aaagacaccg caccctggaa cgaaaaaaat attaaaccaa gaaaaagtag atatgacgaa
    22741 tatggttgct cggcaaatca caccccacga ttttgaaact tttgattgga tcattggcat
    22801 ggatcaagaa aatgtagaag aactgaaacg aagagcacca cgctcagcgc aggggaaaat
    22861 ccatttgttt ttaagtagca ttcctggaaa agaaaaagaa aatgttcccg acccatacta
    22921 taccaataac tttgatgaga catatcactt gatcacagaa ggcctgaatc attggtggaa
    22981 tatttggatt gatgaaaata aaataacaaa caatttgtaa agttaacgct ttctttttaa
    23041 ggcgatgtta gagcatttcc ttgttttttt gtaaaatgtt aggtaaaata aaattaatta
    23101 agtaacactt tcttagctaa aggaggagtg cattcgatga acgaagagac agtatcgcca
    23161 atgaagaagc ttacgaagtt agttactcat acttcgtatc aactttttga ttcttttgta
    23221 aatgacactg aagaacatgc taagaatgta cgattgcaag gaaagtttat tcttgagctg
    23281 caaaaagctg ctactgaaca tagtctagtt atcttacaag taagagcaaa tcgtttgagt
    23341 agcaagtatg aaacgatctc aggctgggtg gcgacaaaaa cggtcgaaaa agaccgagtg
    23401 atgatccgcc tacaaaacga agagcagcag atccggatgg ttcctgtaga acaaattgaa
    23461 aaaataacaa tcctttcacc agcaggggaa caggaacggt tatcaaaata atttatacca
    23521 gatagaaaag cacgtatgag cttgtaccat caatcattac taatcaaaaa taaccgaatg
    23581 ctttaagctg atatcagtaa aacggaacgt tttaggatga gtatcacagc atgacagcat
    23641 tgggttgatt agttgaatcg attggatggt acaagttttt ttgataaaaa aaggtcctgc
    23701 tagccgggga gctagcagga taaggagtta aaaatgaaaa agtgttgtta gggttgtttg
    23761 ttggtatgct tatataatag tcactagttg tgaatatttt gtgacgaaag ttttcgaaag
    23821 ttgttaagtt tttttgcgaa aactattttt atatcatata tatgtctaaa atctatatta
    23881 taaaagaaat gttgtttgat cagcttctct tcggtattag ttcaaaacaa aacggcaaag
    23941 tatatggagc tactttgccg ttttgtttct ctaatactca ggtcagttta tagacgctca
    24001 ataaacggtg ttcaaaagcc agtttctcgg tattaagtca aatttatgca aaaatgtgag
    24061 aagccatttt caaaaaatat ttcttaatac tcgaaactga gcggcttttt cacgacctct
    24121 caataaacag tgttcaagag acagcctatc ggcaataagg ctgaacgtct ctttcacaac
    24181 ctcttttatc aatctgctac ttcttctttc ttagcgaaac tccaaatcag tccgataaca
    24241 aaaccgataa ctgctggtac gatccacccc atccccaatt tgaaaaacgg caagtagctt
    24301 tccgcaacat ataaaagatt ttgaacaata ggtgtttgtt tgatagtttc tgggcaagcg
    24361 ttcaacccat caaaaatcga agcaatcaat gtaaaataag tagtcattcg ataaacagcc
    24421 gtacgctgct taaacaaagg accaactaac actagtaaaa tcagtgtcat tgctaatgga
    24481 tagatgaaca tcaaaacagg caacgaaaat tgaatgatat tcgtcaagcc tacgtttgcc
    24541 gctaaacaag gaaggatact agcaagtgca acaaagaatg cgtaatttct ttgcggaaac
    24601 aaatctgtga atgtttctgc aaaagctgta atcaaaccaa taccggtctt caagcaagct
    24661 aatataacga taaatgctaa taagatactg ccataagtac ctaaataatg atcagcaatc
    24721 tgagccagcg tgatcccgcc attttcactt agagcaaagc tacctaagct catagcacct
    24781 aaataagcga gcaaagtata aatgattccc atcaatataa tgctgatcgc accagatttg
    24841 atcgtgtctt tagcgatttc agcaggtttt gttacaccca tgttttggat tgttgtgaca
    24901 atgatgatcc caaatgctaa agcagcaagc gcatctaatg tgttatatcc ttgagtaaac
    24961 cctgtgaaaa aagcatgctc ttgataattt ggctgaatag gcgcttgatt tacagaacct
    25021 agtggattca aaaaagcaag gagaagcaaa gcacctaata aaaccaaaaa tgcaggattc
    25081 aggaactttc caacataatc taatatcttt gtcggttttc ttgaaaacca ccaagccagc
    25141 acaaaaaata caatactgaa aattgctaga tatagcgtct gtctgctagg atcgataaat
    25201 ggtgctaaac caatctcgaa agatgtggtt gcaaggcgag gtaaagcaaa aaacggtccg
    25261 ataactaagt aaagcaagat ggtaaaaacc atcgcatatg tttgatttat ccgttttgct
    25321 aattcatata cacctgagct tttcgataca ccaatggcaa tcacccctaa gaagggaaga
    25381 ccgataccgg taaccaagaa acctagattc gcccaaaagg tagcagcgcc tgcttcttga
    25441 cccaaatgaa cgggaaagat aagatttcca gcaccaaaaa atagaccgaa aagcatggag
    25501 cctataaaaa gatagtcttt aaatgataat ttttttgtca tacataactc ctttacggtt
    25561 tttttaaaag taacaaaatt tcgaaaaata tgcaacaatt ttcagaaaat ttcctatata
    25621 aaacgacggt tatttaataa cgtaagtgat aaaataaaag cgctgaatca tctgaggagg
    25681 aatcatcgtg acaaagtcga gattagaagc atttactgat ggagtggtag ccattgtttt
    25741 gagagttctt gtattagaga tcaagatccc taatacatct agctttcatt ctttatgggt
    25801 catccgaaat acattattag cttataccat cagctttata tttgttgcgg tgatctgggt
    25861 gaaccatcat cgtatatttc agatggcaga acgaattaat taccgagtgg tctggtcgaa
    25921 tatctttttg ctcttttggc tgactttatg tccttctgtt actagttggg tcgggcgtaa
    25981 ccctgaagct ttttggcctg agatggctta tgtcttagta tacacgatgt ggagtttttc
    26041 ttttggtatc ttgatgaggc aaatcatgaa agcaaataaa ccagattccc atgtcgtgag
    26101 agttttgtca aaggaccgtc gaagtcttat ctccatgatg attaacttat gcttgattgc
    26161 cggtgtcttt tttgtaccgt tgatcgggtt attcggacgt ttttttgtat ctggtatttg
    26221 gattttttcc tataaaaaag cagatgccta ttatcagcgg ttgtttccgg ggaaatagaa
    26281 aagaggctgg gacagaagct tttaactcca agaaataagc cgaaatttca caaaaattta
    26341 agaagcaatt ttcgtaaatt ccttcttatt tctcgaagct gaccacttct gtcacaacta
    26401 ctttcttact tcttacgttc atatttcata tgatagatta gtaatagaag caagaaccac
    26461 acaggtgtta gtaataaagc gattctcgta tcttttccta ggaacaagac gacacacaca
    26521 aaggccatgt aaagaaatac cagataatta ctaaatgggt acaacggcat tttaaatgaa
    26581 tgagcttttt tgccgaggat acttttacga tatttcatgt gcgtgtagac aagcatactc
    26641 cagataaaca agaaacaagt agtggcaata ctgcttacta gtgtgaatac ttcactaggc
    26701 atcacgtaat tcaagataac agaaatgaaa atgacgatag tagaaaatgt caaagcattc
    26761 cctggcacat gatgtgttgt taactttttg aattttttag gggcactgcc ttcttgcgct
    26821 aaagaacgaa gcatacgtcc tgtactatat attgcactgt tgcaggcaga agctgctgca
    26881 cttaatacga ctaaattgat caaggaagca gcaatcgtga tcccaatttc cgaaaatact
    26941 tctacaaatg gactgttatc tggattcaag ctgttccatg gatagattgc cataatgaca
    27001 aaaagagatc ctaagtaaaa taagatgatc ctgataggaa tattattgat agcttccggc
    27061 aacacttttt caggattttc tgtttctcca gcgactaatc caactaattc tatcccagca
    27121 aaagcaaagg ttaccatttg gaaagctaag ataaatcctt ttgcaccagt tggaaagaat
    27181 ccgccatgag accatagatt cgtgatactg gctgtgccaa tatctgtcgt atagtgggtg
    27241 aggatcatat aagctcctac gaggataaag gcaatgatcg ctacaacttt gatcaatgca
    27301 aaccaaaact ctaactcacc aaacaatgag acagccacca tattcaagca aagaagaaga
    27361 cctaatgcaa tcagttcagg aagccactga ggaacaccag gcgcccagta gcgtacatac
    27421 attccgatgg ctgttaaatc ggccattgca atcgataccc agcagaacca atacgtccag
    27481 ccagtgatga aagccgcttt tttgccaaga tattctgcaa caaaatccag aaaagaatgg
    27541 ttattggtgt ttgataacag aagttcgcct aacgcacgca taatgaaaaa acagatgcat
    27601 ccagtaataa gataggcgag caatatcgaa gggccagcta gctggataga ctttccggaa
    27661 cctaaaaaca accccgttcc aatcgctccg ccaatagaaa tcagttggac atgtctattt
    27721 ttcagtccac gctgcagttc ttgctcttca ttcattttta aatcatccta actaaaaaag
    27781 tttaattact tccatattaa aagagttttc tgaaaaacac aactaagatt ccaaaatttt
    27841 cagaaattta ccttttatga gtaattcata aaaaagaact gcggtaaaca ccgcagctct
    27901 ttgtcccaat cagctattga tcctaagcat ttaaaatacg tgaaaggaat tcttttgtac
    27961 gttcttcttt cggatggaca aagatatctt ctggtgtacc ttcttctgca atcacacctt
    28021 tatccataaa aatgacacgg tcagatacat cttttgcaaa ttccatctca tgcgtcacga
    28081 taagcattgt caagccagta tgtgctaagt ctttgatcgt attaagcact tcgccaacca
    28141 tttcagggtc taacgccgaa gtaggttcgt cgaatagcaa tacatctgga ttcattgata
    28201 atgcccgagc aattgcgaca cgctgtttct gtccaccaga taattgtgct ggtttggctt
    28261 cgatataacg atccatacca actttttcca gattttcgat tgctactttt ttggcagttt
    28321 ctttatctcg tttcaggaca gttgtttgac cagacataca gttttccaag acattcatat
    28381 tgttgaacag gttgaaggat tggaaaacca tacctagatt cgttcgatat ttaggtaaat
    28441 tatatttagg agctaataca ttttctccat tataaatgat ctcgccgcct gttggttttt
    28501 ccagtaaatt gatacagcgc aagaaagtag atttaccaga accggaagaa ccgatgatcg
    28561 tcacaacttc acctttattg actgttacgc tgatatcttt caatacttca ttttcaccga
    28621 agctttttct tagatgtttg atttcaataa ttttttcatt catgattctt cctccttatt
    28681 cctgcaaatt tgaattgtcg atttcttcaa gcttggtata agctgaagga ccatcgattt
    28741 tcttttctac cgcccgcagg attctggtga tcgtaaaggt catgactaag tagatgatcc
    28801 cgacgatcgt aaatgtttgg aagaattgga aattcgcacc ggaagcagag tttccttgga
    28861 agaacaaatc agctacgccg attacactta aaactgctgt atctttgata ttgataacaa
    28921 actcatttcc tgttgctggc aagatattcc gtaaaacttg tggaataaca acttttctca
    28981 tcgtttggcc atgggtcata ccaatggctt gcgcagcttc gaactgccct tcatctactg
    29041 caaaaatccc cccacgaacg atctctgtca tgtaagctcc agtattgaga gagacaataa
    29101 acaaagcggc aatcgttcgg ttcaatgaaa tgccaaaagc gagtgccaga ccgtagaaga
    29161 tgaccattgc ttgtaccatc atcggtgttc cgcggaatac ttcgacatag atagaaagaa
    29221 gtgcatttcc gattttttgg aagaagcgag tcagtgcatt tcctgattca ggtaccgaac
    29281 ggaagacacc aatcagtaat ccaagtgtag taccgacaac tgtcccgatc aatgcgatga
    29341 acaaggtaag tccagcacct cgtaagaaca tattgccgta ttgtttccaa atcgtagtga
    29401 aatcagaaat gatgccaggt ttttcttcac tatcagttgc agcaggctga tctttgactg
    29461 cttggtccat aattttggtt cgttcatcgt tagagatgcc agaaagaatc tggtttactt
    29521 tttggatatc cgggtcgcct ttacgcatac caacagaaat ctgaacatct tccgggtttg
    29581 tctggaagcc atctgcaccg ctgaattcaa gcatttccaa gtctttgttc acactagttg
    29641 ccgttacacc ttccggacgt tcgctgacat aaccatcgat cattccagaa gctaaagctg
    29701 ttctcattgc tgagaaatta tccattgctt gttgtttctg cacatctggt atctgatcga
    29761 tcacagtata gtggaatgta tttaactgag cagtaatctt tgctccagaa agatctgcta
    29821 aactttttgc ttttgcatat ttgctatctt tttgaactac tactactaat tgtgattcgt
    29881 aataaggatc ggtaaaatca atttcttttc gtcgttcagc agtagggctc attccggcga
    29941 tgatcgcgtc gatcttgcct gattgaaggg caggagccag accatcccat ttcgtttgga
    30001 cgatgactaa ttttttccct aaaccatctg ctactttttt agcgatttga acatcataac
    30061 cgccggcgta actattttgg ccttgaattg gcaaggcatt attctcatct gtttgttgag
    30121 accagttgaa gggtgcatac ccagcctcca tacctacgcg aaactcatcg ctcggtgttt
    30181 tttcatctgc atttgccaga gatcccacac tgaatagtga gaagacaaaa gcaaatagta
    30241 cggcaactga aaccatattt tttttcattt ttttctccct ttctgattat atgatacacg
    30301 agcggtctag atgaaatgcg gtaaagcttt atctgaaaaa ataaaaagcg accgatttat
    30361 agatataaaa aggccgctaa aattccataa ggtgataaac ctctcacaaa acatagcgca
    30421 acttagcatc actgctaaga cagtccgcaa gctcttaact tgcgtcccaa catacactcc
    30481 aagcaaaaaa gtgcacattt cggcgatcct ccctattcct gcttctccat gtttgcttca
    30541 ctccacaggg ttaatgattc tcgcgacctc tacctcattg attgaggcat ctcgtattca
    30601 gttatttata caaaaaaagt ttacgatact cgagaggctt tgtcaatcta taactgctgt
    30661 tttttgaaaa aatactgata aatatagaaa tctttacttt taccgaaaaa acaatcaaaa
    30721 tgtgaatcat ctatccattg caaacgatag tataattata cacaaaaatg tatgtaatac
    30781 aataaaaaaa gaactagaac agaaaatttc ctgtgtccag ttccatctcg atcatttaag
    30841 ctgccgcttt ctttggctca cgtcctagga tcgcttgcaa aagaacaacg acgccataaa
    30901 tggctaatat tagataaatc aaacttgagt attgggtgat tccccagccc catgttcgat
    30961 atacggctaa tgtaatggct aaaacaattg cggcaatcac gttgaataaa gcaaaagccc
    31021 aaagatttcc attttctttg cgtacaagat aaaaaacaga gaaataaacg cttaacgcta
    31081 accaggcgat acaagccaga ataacgcccc aataaataaa gaatggtaac atatcgattc
    31141 acctcacttt tatacagcag tattgtaaca cgttttcatt cttttgtcag tgaacattgt
    31201 gataaattgt tcaaatgccc ataacacttt tttattccca tttttctgac ttttcaacaa
    31261 taaatttgtt cgagtatggg ctataatgga aagtgaggtg acaaaacatg aaacaattaa
    31321 cagtagacga agcagcaaaa gagatattag cttatcagca aattacattt ttgaccggtg
    31381 caggggtatc cacaccttct ggagtgccag actatcgatc tttgtcagga atctaccacg
    31441 ggatggagca gccggaatac ttgctgagtc atcaagcaat ggtgaatgaa ccggataaat
    31501 tctatcaatt tgtcaaaaag atttatcatc ctgaagcaaa acccaatatc attcatttgg
    31561 aaatggcaca tctagcgaaa gaaaaaaatg tttgggttgt ttcccaaaac atcgacggtt
    31621 tgcacgcaaa ggctaaaagc ccgcaactcg tcaattttca tggaagtttg tatcaatgtc
    31681 attgtcgaaa atgtcaacaa acggtagact ggaaagaata tcttcacagc gacaaacatc
    31741 aagcatgcgg aggacaaata cgcccagaca tcgtgttata tggtgaaggg tttcaagatg
    31801 gagtcatgga gcaggcggct tatgccgtca gccaagcaga gctgatcgtc attgttggaa
    31861 cgagttttca agttcatccg ttttgtgatc tggtacagtt tcgccagccg tcagccaaaa
    31921 tcttagtaat caatcaaacg cctgtttatt tagcggagcc ttatacgttt attcaagaag
    31981 acggagcggt tgttttcaaa aaaatacaag aattaggagc agattatgac tgaattcaaa
    32041 accgaaaaag aaaaaatgat tgcaggcgag ctttattttg ccaatgatcc tgagctggtt
    32101 gccgaccgta tgtttgcaag aagtcaaagc caaatcatca accaagcaga atcagcggaa
    32161 ctgcgatccc aattgttgaa agagactttt ggccgtacag ggaaaaaaat ctatatggag
    32221 ccagttatca attttgatta cggatataat atatttgttg gggagaattt ttatgcaaat
    32281 ttcaattgta catttttaga tgtcagcacg attgaaatcg gtgataactg catgttcgct
    32341 ccgaatgtcc agctttacac ggcgacgcat ccactacatc cagtgaaacg taacagcggt
    32401 ctggagtacg caaaacccat taaaatcggt aataatgttt ggttaggcgg aggagttatc
    32461 gtcacgccgg gtgtcactct aggggacaac gtagtagtcg gcgcaggttc ggttgtgacc
    32521 aaatcttttc ccgacaatgt agtgattgca ggaaatcctg ctcgtatcat caagacagta
    32581 gaggaagaac taacagaaga gtctcttgaa acattacgtc ataagatcga tatgatcgat
    32641 cgagagttga ttgctttatt agaaaaaaga atggatacgg taactaaaat aggacaagtg
    32701 aaaaaagaag aaaaccaagc agtttatgat gcaaaaagag agcagcaagt attggataaa
    32761 gttgttagtc tgctgaaaaa taagggatat aaggaaacaa taacagatac gtatgttgat
    32821 ttgatgaaac actcgcgcga ataccaaaac aaaatgaagg aggaataagt gtgatcgaag
    32881 gagagacacg gcgaaaacaa atcatgcaaa ccttggcaaa tacagaaaaa ccagtgagtg
    32941 ctagcaagtt tgcgaaaact tttggagtca gccgtcagat cattgtaggc gatattgctt
    33001 tactacgagc tgcaggtaaa agtatcgtgg caacagcaag aggttatatc ctcgaaacag
    33061 aagaaaccaa tactgggcac atcagtaaaa tcgctgtcca gcatggaaaa gatcaaacag
    33121 aagaagaact gagactaatc gttgaaaatg gtggtgaaat cattgatgta attgtggaac
    33181 atccccttta cggtgagtta gcaggaactt tgcacatcaa aacgccacaa gatatcgatt
    33241 cctttatgag acgttatcaa aaaagtaatg ctactttgtt atccgaatta acaggcggca
    33301 tccatctgca tacgatccgc tacccagaaa agcatatgct gaaaaacatc aaacaaaaac
    33361 tagctgaagc aggaatcctc tacgaaggat aacatcgtag ctgctacttt ttatcctaga
    33421 aactattcta tcatctctga gataaacacc catatggaag aaataagaaa aaataagaaa
    33481 agatgcgtat ccaatacaca gaattttttg gaattgcgat ttctagatgt ctatctaaat
    33541 ttcgattagt ggagaaacat aaaaaaggga agtgcagctt ttgagcactc ccctttttta
    33601 taaagctttc ttgtagcata gtatcgaagg aacctgccaa ttctctactc gatcaccaat
    33661 acgccttctt ttagtgcaaa tggattttga tcgttgatgt ggtcatagaa catcacaccg
    33721 ttgatatggt cgatttcatg ttgaacgacg atcgattcat aattcttcaa acgaatcttg
    33781 tgtttttcac cattcatatc ataataagac acagtgattt tggcatggcg aacaacatat
    33841 cctggtactt ctcgatcaac agataaacag ccttcacctt cccctaaaca agcatcttga
    33901 acagaatggc tcaaaatttt aggattatac ataacagtac ttagagaagg tccatctgcc
    33961 tctgggtcag gacttggtac gtgaacagca atgattcttt tagaaatatc taattgagga
    34021 gcagctaagc caacaccgcc tcgtaagtgt agttcttcag ctttgattgg atcttggctg
    34081 ttttttaaga actcaagcat ctcttttcct aaagagatat cttcttcgct taatggaaga
    34141 gaaacttctt ttgcaacttc gcgtaaggtc ggatttcctt cacgaatgat atcatccatt
    34201 gtaatcatgg aatatgtacc cctttcttaa aagactgtgt ttcaagtcta ttttcgcatt
    34261 atagtttacc acaaaaatgg aaaagtagta gtaagattgt ttgaaaacta ccttttctta
    34321 atgattaact acttgatttt caacccggtt tcaggtaaag tatagctgtg tgctctaggc
    34381 acatgacctg ccacttggtt aagcgaatca ccaacgtttt actcagtcgc aggggaataa
    34441 tcgaaagagg tgaaagaaat ggcaatttca aaagaacgca agaatgaaat catcaacgaa
    34501 tacgctcgcc atgaagggga tactggttct ccagaagtac aaatcgctgt attaactgaa
    34561 gaaatcaacc acttgaatga acatgcacgc gtgcacaaaa aagaccatca ttcataccgc
    34621 ggcctaatga aaaaagttgg tcaccgccgt aacttgttag cttacttacg taaaactgac
    34681 gttcaacgtt accgcgaatt gatcaaacgt ttaggcttgc gtcgttaatt ttagtcaaaa
    34741 agagtgggat tcacatgaat ctcacttttt ttactcgtat atcaattaag taaaattccg
    34801 tgatcaagct agatggactc ctactaacca aatgaaagct gctcaaaaga cggctttcgg
    34861 cagttttcat ttgtttagta gcgtcttgct ttcacgagaa ggaaaaggag aagttatgtc
    34921 agaaaaacaa gtattcaaaa cgacatgggg cggccgtccc ttacaaatcg aagtcggtca
    34981 gctagcaaaa caagcaaacg gagccgtttt agtccgttat ggagatacag tagtcttaag
    35041 tgcagctgtg gcctctaaag aagcaaaaga tacagacttt ttcccattaa cgatcaatta
    35101 tgaagaaaaa atgtatgcag cagggaaaat ccctggaggt ttcatcaaac gagaaggccg
    35161 tccaagtacg gaagcaacat tgactgcccg tttgatcgac cgtccaattc gcccgatgtt
    35221 tgcagaaggc ttccgtaatg aagtccaagt aacgaacatc gtgatgagtg tagaaacaga
    35281 ctgctcgcca gctatggcag caatgctagg ttcttcttta gcactatcaa tctcagatat
    35341 cccatttgac ggcccaatcg caggcgtaga agttggtcgt gtaaatggcg agtacgtctt
    35401 gaatccaaca gtagaacaag cagaacaaac agatatcgaa ttgactgttg ccggaacgaa
    35461 acaagccatc aatatggttg aaagcggcgc gaaagaagtt tcagaagaag acatgcttgg
    35521 tgcattgctg tttggttttg acgctatcaa agagttagtt gctttccaag aagaaatcgt
    35581 tcaagcagta gggaaagaaa aaatggaagt gactttactg caagttgatg aagtattgaa
    35641 aaaagaaatc tttgatgctt cttacgctac aatgaaagca gcagtaatga ctgaagaaaa
    35701 acttgctcgc gaagataata tcgagcaggt gaagatcgat atccgcgaag cttatgctga
    35761 gaaatttgca ggtcatgaag atgaagatca cttgttgaaa gaagttaaac aaatcacaga
    35821 agatcttgaa aaagatgtcg tacgtgaatt gatcaccatc gataagatcc gtcctgacgg
    35881 gcgtaaattg gatgaaatcc gtccattgtc atcagaggtc agcttattgc cacgtgtaca
    35941 tggatcaggt ttgttcacac gaggacaaac acaagcttta tcagcttgta cactcgcacc
    36001 tctaggagag caccaaatca ttgatggttt aggggtggaa gtcagcaaac gattcatcca
    36061 ccactacaat ttccctcaat tttctgttgg ttctacagga cgtgcaggct ctccagggcg
    36121 tcgggaaatc ggacatggcg cattaggtga acgtgcgtta gcacaagtaa tcccttcaga
    36181 agaagaattt ccttatacga tccgtttagt agcagaagta ttggaatcaa atggttcttc
    36241 ttcccaagca agtatctgtg ctggcacatt agcattgatg gatgcaggtg taccaatcaa
    36301 agcgccagtc gccggtattg cgatgggact tgtttctgat ggtgaaaact atacgatcct
    36361 aactgatatc caagggctgg aagatcatct tggcgatatg gactttaaag ttgccggtac
    36421 aaaagatggt atcactgctt tacagatgga tatcaagatt caaggaatca cagaacaaat
    36481 cctaactgaa gcattaactc aagcaaaaca agcacgtatg gaaatcttag aagaattgac
    36541 ttcaacgatt gctgctccac gtgaagaatt aagccaatat gcaccaaaaa tcgaaatgat
    36601 ccagatcgaa cctgctaaaa tcaaagatgt tatcggtaaa ggcggcgata cgatcaacgg
    36661 aatcatcgat gaaactggtg tgaaaatcga tatcgatcaa gatggaaaag taagtattgc
    36721 ttcagctgat gcagaaatga tcaaaaaagc tatcaagatc atcgaagatt tgaccaaaga
    36781 agtcaaagtt ggtgaagttt atctagggaa agttgtccgt atcgaaaaat tcggtgcatt
    36841 cgtcaacttg atcaaaggaa aagatggact tgtccatatt tcccaattag caaacgatcg
    36901 tgtcaataaa gtcgaagatg ttgttaaact aggtgatgag atccttgtga aagtaactga
    36961 aatcgataaa caaggacgcg taaatctgtc aagaaaagca atgttgaacg aagatggttc
    37021 aacgaaaaat gaagaaaaat aatttaattc ataggaataa tgtacttgtg atcgtgaagg
    37081 tgacaggggg acaataatta ttcgaaaaaa ggggactgtg acaaaagccg tgtcacagtc
    37141 cttttttttc gaataaacgg tgttcaaaag cttgcatctg cggtaataag tccagccaag
    37201 ttaaaaacat taggagctgt ttttatttgg ctgttcttat tacttgatgc agaacagctt
    37261 ttttacaacc tctccaataa acgatgttca atcagctgtc cctcgatatt agctcaaaaa
    37321 acggcaaaat atgaggaact atttttccgt ttgtttctct aatactcagg tcaaaccgct
    37381 gatttcacaa cctctgattc acggtgttcc agaagcagct tctacggaaa taagctgaac
    37441 acttttgtta caacttcttt ttttctggag tttcttggcg catatttttg gagtccttct
    37501 gaataaagcg taaaataaaa gtgagaaata tggataaagg agggaaagga gatgctgaat
    37561 tttttatggt cgttatttat cggcggtata ttaggtttga tagctggcgc aattttagga
    37621 aaggaagtcc cgggaggcgt atctggaaat atcattatcg gttttttagg tagttggctc
    37681 ggcgaatttt tactaggacc actgggaccg gaactcggcg gtttttattt gataccagca
    37741 ttattgggtg caattttttg ccttgctctt tattcatttg tggcaagacg tatcagagaa
    37801 cgtccaaaga aagaataatt ttattcaaat aagttgacac ttcagtgtgt tacttggata
    37861 attagaagca atttaggaaa gcgggtgtca tggataagaa tatcatctat cattcaagct
    37921 tacctaaagg atatccatat aacaagaaat gagggaaaaa cgtggcaaca acgcacaata
    37981 ccaaaaagat atcaatgact ggcctgcttg tagcgatggg agtcgtctac ggagacatcg
    38041 ggacaagtcc gctttacgtt atgaaatcga ttgttgaaga taatggcgga ttgtcaggaa
    38101 tcaatcctga ttttgttatc ggatcagtct cattgatttt ttggacattg actctactga
    38161 caacaatcaa atatgtcgtg attgcattga atgcagataa ccatggtgaa ggtggaattt
    38221 tctcactcta cacacttgtc cgaaaaggtg ggaaatattt gatcattcct gcaatgatcg
    38281 gaggagctgc cttactagca gatggggtat tgacccctgc tgtgacggta acgactgcga
    38341 ttgaaggctt gcgaggaatt ccgcagtttt ttgaccggtt cggtaataac cagaacgtga
    38401 tcgtcattat cactttagtt attatttttg ttttattcat ggtccaacgc tttggtacag
    38461 atattgtcgg aaaagcattc ggaccaatca tgttcgcttg gtttactttt ttaggcgtaa
    38521 tgggattaat gaacttcagt caagactgga cagttatccg tgcactgaat ccttattatg
    38581 ctatccagct gctttttagt gcagataaca aattaggctt gttcattctt ggaaatattt
    38641 tcctggctac gacaggtgca gaggcgcttt attccgatct tggacatgtc ggcaaacgaa
    38701 atattcgtct gagctggcct tacgtcaaaa tttgtttggt tatcaattat tttgggcagg
    38761 ctgcgtggtt gttgaatgtt tatcaaaacc ctgaagcaca gcaaatcaag aacctgaatc
    38821 cattcttcca aatgatgcct caaagttgga tcgtgatcgg cgttgctttt gcaacagtcg
    38881 ctgcagtcat tgcttcgcaa gcattgatta caggttcgtt tacgctagtt tcagaagcaa
    38941 tcaagttgaa actgctgcct cggttaaaga tcatgtatcc tgggaacagt atcgggcaaa
    39001 tgtatatccc agcagtcaat ctgattttat ggatcgcctg ttctctagtg gttgtcacat
    39061 tccgaacgtc tcatcatatg gaagctgctt acggtctatc gatcactgtg acgatgttga
    39121 tgacaacagc actgctttac ttttatctta tccaaaatgg gcattctcgt tggcttgcat
    39181 atttagttac ctttttcttc ggtgcgatag aagtcgtttt ctttatctca agtgttgtca
    39241 aattcttccg aggcggtttt gtagccgttc tgattggttt gatcatctta gtcgtgatgt
    39301 ttatttggga acaagggaac attattcgcg aatcgtcaac cgaggatgtg tctttgagag
    39361 attatatccc gcagcttaga gaattacgtg atgatacttc tttaccaatc tatcagacaa
    39421 atgtggtctt cttggttcct gatatggctg aagataaagt tggccgacag ttcatctatt
    39481 cgattttaga taaacggcct aaaagagcac gagtctattg gtttgttcat gtggaagtga
    39541 cggatgagcc atatacgaaa gaatatcaag tagatatgat ggacactgat tttattgtcc
    39601 aagtgaatct gttcttaggt ttcagggtcc aacaggaaat caacgtatat gttcgccaag
    39661 tcatccatga tttgatgaaa caaggccgtt tgccaaaaca gccacaaaga tactctctga
    39721 caccagggcg agaagttggc gatttccaat ttgtcctgat tgatgaagta gtttccaatg
    39781 tgacaactct aggaaaatgg caacgtcaaa tcatgcaggc aaaactggca atcaaaaaaa
    39841 tcgctacctc gccagaaaca tggtttgggc tagagtacag cgaagtaaaa catgaaactg
    39901 ttccgcttat cattggaaat actcggaaaa catggttgaa agagcggttg tgaaagtagc
    39961 gctttgacct gaactaatgt cgaatgtcag ctacttgaac accgtttatc agaggtcatg
    40021 aaaaagctgt actgcaacaa gcagtaagaa cgagcagata aaaacagctt ctcatgtttt
    40081 tcatctgttt gggcttaatg ccgtagatgc aagcttttga ataccgtttt tatcagaggt
    40141 catgaaaaag ctgtttagca acaagtaacg caatgatttt gaagtgataa ggagatgtgg
    40201 ctgaaaaatg ccgcatctcc ttttttattc ctaaatatga atacgcatta ttttcaatct
    40261 ttttgtagaa aaagggtaca atagaaaaag agaaaggcgg gagaatatgg ttatcaattt
    40321 cattatttta tttttgatga ttgcaattac ttcatttttt gtagcaagtg aatttgcttt
    40381 agtgaaaatc agacgatcaa gattggaaca actagaaaag gaaaatgtaa agaacgccaa
    40441 gctagctctt catgttacac accatttaga taactattta tcagctagtc aattaggaat
    40501 tacgctaacc ggtttgatca tcgggtgggt cggtgaagga tctgtcgcgg ctttactaga
    40561 accagtgatc ggtaaactac cttttagttc ggctattagc agtacaatct ctgtggcgct
    40621 gggatttatt ttagttacgt atatcgatgt ggttattggc gaactgcttc ctaagagcta
    40681 tagtatcgtc aatacggaga aggtcgtctt gttcgtagtt aaaccacttc attattttta
    40741 taaagtaatg tttcctttta tttgggtatt gaatcattca gctgcagggc tagggaagct
    40801 attaggtgtg cgtctcgtat ctgaaggaga agagacattg tctcaagaag agctgacatt
    40861 agtcgcattg aattcttatg aaaaaggaga gttgaccaaa gaagaatatt attatttgga
    40921 aaacgtgttt gaatttgatg ataccttggc aaaagatata caagtagacc gtacgtccat
    40981 gcaagtattc gaagcttcag aaaccgtcaa agaagcgata aaagcttcga ttgaacaagg
    41041 acatactcgc tatccggtta tcttagaatc aaaagacaat gttttaggtt atgtcacctt
    41101 gccagatttg atcaagcaat cttataaaga tgaccagctg acagtagaac agttgattga
    41161 agagccgatc gtaacaactg aaacgatccc tataaaaaaa ttattgacga tcatgcgaaa
    41221 aaagggtaag catattgcta tcttaaaaga tgaatacgga gggacaagcg gcttagtgac
    41281 gattgaagat attttagaag aaatcgtcgg ggaaatccga gatgagacag atttggacga
    41341 agcattgata gcggaacagt cagatggctc ttatatcatc tccggcaaac taacattaga
    41401 tgatttccag cgttattttc atgtagagat tccagagttt gaagaaacga attttactac
    41461 acttgcgggc tttgcttcta gccgatataa agaaataaaa gcaggaacaa tcattgagat
    41521 tgcatcattc cgtttcacgg tattagaata ccagcatgca catatcgatt atttcaaagt
    41581 agagtccacg gaaagaaaaa cagaataaaa gaaaaaaggc tgtgacattt tttgtcacag
    41641 cctttttgtt atctaaaaaa cctgttttcg gtaaacgaag aagatatttt caataaaagt
    41701 tttcattttt agccgcaacg atttctttta atcaaggcta tcttgcttct gcatgttcta
    41761 atgtgaaatg ttcctcttca cccatgtgtt tcaattgttt catcgaacgc atataagagt
    41821 aaagcaacac ggcaacagaa ccgatccaag ctaaaggaga agctgctgct gctcctgggt
    41881 atccccacat tcctgtaaga acgatagctg caaaagaacg cataagcaat tccatcatac
    41941 cggcgatcgt agggatcttg ctttgtccaa gcccttgaag tgtgtagcgt aaaatgaaca
    42001 ggactgctaa gatccagtaa agagacccat tgatattgaa gtaggtttgt gctaattcaa
    42061 atacacgtgt ttctgatggg ttaacaaaca attgaacaaa gaaatgaccg aagaaaatga
    42121 cgatagcacc tgctaagaga ctaaatccga cactcatcag taaagtttga cgtacccctt
    42181 gcaagattcg gccatattct ttcgcaccat agttttgtgc agtgaaagtt gccattgcaa
    42241 taccaaatga catcatcggc tgatttgcaa attggtcgat acgaccagca gcggcttgag
    42301 cagctacgac atctgtccct agactattta gtgctgcttg taacacgata gcaccaacag
    42361 cgatgatcga agattgaaac gccattggca aagcagcatt taggtgtaca gcaaactctt
    42421 ctttttcaaa tttgaaatct tttttgcgta actgtaacaa aggcattttt ttcttgatat
    42481 acactagaca cagtacgctg gaagaaactt gtgcgattac tgtagcaata gctgcacctt
    42541 ccacacccat gccaaaatag ataataaaga tcaaatcaag tacgacgttg atgatgaccg
    42601 caatgatcag aaagaatagt ggtgtgcggc tgtcaccaag tgcgcgaatg acattagaaa
    42661 gcaaattgaa actgactgat gcaaagatcc ctaataaaat aattgaaata aatgtttgtg
    42721 cttgatcaag tatttcagga ggagtttgca tcaattgaag catcggacga ataaacagga
    42781 gacttaggac agttaaaatc acagtgacga caagactaat gacaacacta gcagcaaaac
    42841 ttttcttcag accccgataa tcttttgcac cgtaacgttg tgcggtaata atggctaaac
    42901 cagctgttaa accttgggca aagccaatga tcaaaaatgt caggcttcca gttgcaccaa
    42961 ccgctgccag cgcattttta cctagcgtct gacccacgat gatcatatcg atcatattgt
    43021 agaactgctg aaaaacattt ccgatcagta aaggaacagt gaacatcaaa atcagtttag
    43081 cggggcttcc ttttgttaaa tcgcgcatct tttaaactcc ttatatcttt aaaatttagt
    43141 tagtagtata tagcgtttat cctgagaata ccaattagaa atcaatgata atttatgaaa
    43201 ctccataaaa agaagccaag aattgagtca aagaatcaac aacttggcct ggtctgtaaa
    43261 ataagggaca tccacgttta tctatcacct taatattgtc taatctcact tggtaaaagg
    43321 atcgtttctc tcgtaccgtc atcataggcg tatacttgag caggaaattg gttcgaatag
    43381 gcaaacggca aatcaatatc catttccaca ttgcttcctg cttgagaaaa atgcatcgtc
    43441 acttttccac gattttcgat caaatcaaag gttaatatat tttctaatgg aatgactccc
    43501 ttaagatcta ggtcaataat aaaccatata ctgtcaatca attcatctgg caaacttgat
    43561 acgacaccaa ctgaagcgta acggttgcgg ctgctatcaa aactttcaaa catctgttta
    43621 acctccttcg aatcaacgaa tgttttattt ttataattaa tcctaggata aactagaaat
    43681 acttacttca acgagggttc attctaataa agaatcagga aaaatgcaac aataattgaa
    43741 tacttttttt caaagtataa atttttataa aggtacgata aaagtatgcg aggaaaagct
    43801 tggtaaatta ggatttttcg tttgtaaagc catttacaaa agtggaagcc ccatatgaaa
    43861 ataaacgatt agaaataaaa aaggcgtgtt aaaagctgct gacccgccgc ttctaacacc
    43921 acctgattgt ttcagtatta ttcttctgtt gaaccgatca cttctggttc gccaccttca
    43981 cctgtgcttt cttcttcttc actttgagcg actacagcag cgatttgctc ttcactgtcg
    44041 gtcacgaatt tgtaatcgtc gttttttgga agatcggcta ctgtaataga atcacctaat
    44101 tccaagtttg tcacgtcaac ttcaattcgt tcaggcaatt tgtctggtgt agcagatacg
    44161 actactgtat acaagttttg agctaacact ccaccagatt tagcacccac tgattcgcca
    44221 acaagaacga cttctgtttc gacttctgtt tcttccgaca tgtttacaga tagaaattct
    44281 gcatgttcga aatgattggt aaaagtgttc gtttggatct tgtgaaccaa tgtattgact
    44341 ttttgtccat cgatgtccat cgtgatgact gtgttcgctc catgttcacg caacaatttg
    44401 cttagtgtca aaccatcgaa tgagataggg gtactttcta ctttataccc ataaacaaca
    44461 gctggaactt tcccttcgtg acgaagtttg tttcttaatg aacggggacg tacttctctt
    44521 ttacttactt ctaatgatac tgacataacc aaaattcctc cttaaaatgt gactgtctgc
    44581 tttggtaacg gacagatttt atttaagtga attctaaagg ctaatctcgt gcacatcatc
    44641 gaaggcaggg cgcttaaaaa agcgcaaaag gagagatgcg cgcatctccc cttagaaagt
    44701 caggattcaa cttttctcca ccaggtcatt acgctgtgta tgagaaatcc aattcacttt
    44761 actatttaac cgtaacgtga actgttaatt aagtcaagga ataactatga tgatgtattt
    44821 gcaagcaaga atattcaagg tataattaat tagtagtaat aagaaaggaa acagcatgcg
    44881 tttagataaa ttaatagaag aaaaattaga aacgacacgc aaggaaatga agcggctgtt
    44941 tgctttgaag caagtgatga tagatggcac agtcgaattt cgacaaaatc gaaatgtaga
    45001 tagtttactg catcaaatcg aagtagcagg tgaacactta gaaacaaatg agtcctatta
    45061 tcttctcaat aaacctgaaa aagctgttac agctgtatca gatccagaaa agacgacagt
    45121 aatcgatctg attgcaagtg ccgatcgttt ccaaccatta tatccagtgg gaagactaga
    45181 tagagataca acaggactat tgctgatcac ttcgaatgga caattgggat atgaaatgct
    45241 acttccagaa aaaaaggttt caaagactta tcaagtagtt gtgaacgaaa gggtgacacc
    45301 ggcagacgta acagcatttg aaaatggaat cgtattcgct gatgggtatc aatgtaagcc
    45361 ggctgattta aaaatactca aagcagacaa gcaccaaagt acggttttat tgacgatcca
    45421 agaaggaaaa ttccatcaag tcaaaaagat gttcttagcc caaggaaaga aagtgaccgc
    45481 attgaaacgg ctaagcatgg gaccattgga attagatgaa cagctaggag taggcgagta
    45541 tcgatcgttg acattagcag agatgaaaaa gttaagtatt tattttaaat aggaggtcaa
    45601 aaagatgaaa tataaaactt tattgtttga tgtagatgat acattgttag attttcaatt
    45661 aactgaagcc aatgctttaa gtgctttgtt tgcagaacaa ggaatcacat tgacaccaga
    45721 aatcgaaacc agctataagg ctttgaatca tcatttatgg caggagtttg aaaaaggcag
    45781 actatcacga gaagaaataa taggcagtcg ttttgggtta cttttccagc aatttgggaa
    45841 aacagtagat agtgatcaaa tggaaaaacg ctaccgccac tacttgaacc aaggtcatga
    45901 tcttgttcca ggaagtctag aaatcctgaa aaaggtccat ccgcatgctg atctttatat
    45961 tgtgacgaac ggtgtgtcgc acacgcaaca tcggcgatta acagatgcag gaatgcttga
    46021 ttacttcaag gatatctttg tctcagaagc tgtcggagcg caaaagccga tgaaagaatt
    46081 ttttgatcat gtcttttccc acattcctaa ttttgataaa aagcaaactg tgatcattgg
    46141 tgattctttg acttctgata tcaaaggagg aaatatggca gggatcgata cgatttggtt
    46201 caatagaaat aaattaccag aaatcgaaga aatccagcct acttatcgaa tcgattcatt
    46261 agaggaactt tattcattat tggaaattga aaaggattaa ttttcagaaa aatatagcat
    46321 tttcgtgttt tttcgctata atagattaga atgaaaaaac ggatacgaaa gaggttttag
    46381 tcaatgacaa aaagcaagcc aattatcatt ggtgtgacgg gtggatccgg aagcggaaag
    46441 acaagtgtca gccgtgcgat tttcaacaac ttcccggacc attccatcat gatgcttgaa
    46501 caagattctt attacaagga tcagagccat ttgagttttg aagaacgatt gaacacaaat
    46561 tacgatcacc catttgcctt tgataatgat ctgctgattc agcatgtagg cgacttactg
    46621 aactataaag ccattgaaaa acccgtgtat gactatgtag cgcatacaag aagtcaggca
    46681 acgatcattc aggaaccaaa agaagtgatc attctagaag gaatcctgat tttagaagac
    46741 gaacgtctgc gtgacttgat ggatatcaaa gtatacgtag atacagatga tgacattcgt
    46801 atcattcgtc gtatcaaacg ggatatggaa gaacggggac gcacattgga ctccgtgatc
    46861 gaacaatatt tgacagttgt taaaccgatg tatcatcaat ttatcgaacc aacgaagcgc
    46921 tatgcggata tcattgttcc agaaggcgga gaaaaccacg ttgctattga tttgattaca
    46981 acaaaagtag ctagtttctt aaaccataaa tagtgcgcag cgaaacagtt gaacggactg
    47041 cgaccgtttg aaaagcaagg attctgcttt ttgaacagtc accagccaac tgttttttta
    47101 tttataacaa gaggttgtga aaacagcatc ttgacctaag tattaaaaag aagaaacgga
    47161 aaatagctgt ttatatttta ccgttttttg agctaatacc gaaggtcagc tgattgagca
    47221 ccgtgaagta gaggttgtaa aaaagctgtc tttgcatcaa gcgataagag catccaaata
    47281 aaacagctcc tcacgttttt cgttgggttg ggcttattat cgtagatgca agcttttgaa
    47341 caccgtttaa cgagaggttg taacagaagt ggtcagatgc aataaataag aagaaactta
    47401 cgaaaattat ttctcgaatt tgtgtgagat ttcgacttat ttttttgaag aagctctttt
    47461 gtcgcacccc c
//
LOCUS       DACDIG010000013        47219 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.013, whole genome shotgun sequence.
ACCESSION   DACDIG010000013 DACDIG010000000
VERSION     DACDIG010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.013"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            225..2369
                     /locus_tag="IV247_000805"
     CDS             225..2369
                     /locus_tag="IV247_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8776896.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(2421..3461)
                     /locus_tag="IV247_000806"
     CDS             complement(2421..3461)
                     /locus_tag="IV247_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP8776897.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(3738..5540)
                     /locus_tag="IV247_000807"
     CDS             complement(3738..5540)
                     /locus_tag="IV247_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8776898.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(5554..6306)
                     /locus_tag="IV247_000808"
     CDS             complement(5554..6306)
                     /locus_tag="IV247_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8776899.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(6315..6677)
                     /locus_tag="IV247_000809"
     CDS             complement(6315..6677)
                     /locus_tag="IV247_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP8776900.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(6853..7725)
                     /locus_tag="IV247_000810"
     CDS             complement(6853..7725)
                     /locus_tag="IV247_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAP8776901.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(7776..8264)
                     /locus_tag="IV247_000811"
     CDS             complement(7776..8264)
                     /locus_tag="IV247_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP8776902.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            complement(8402..9232)
                     /locus_tag="IV247_000812"
     CDS             complement(8402..9232)
                     /locus_tag="IV247_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAP8776903.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            complement(9248..9856)
                     /locus_tag="IV247_000813"
     CDS             complement(9248..9856)
                     /locus_tag="IV247_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAP8776904.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(9849..10166)
                     /locus_tag="IV247_000814"
     CDS             complement(9849..10166)
                     /locus_tag="IV247_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8776905.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(10322..11050)
                     /locus_tag="IV247_000815"
     CDS             complement(10322..11050)
                     /locus_tag="IV247_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAP8776906.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            11209..12090
                     /locus_tag="IV247_000816"
     CDS             11209..12090
                     /locus_tag="IV247_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8776907.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(12327..13370)
                     /locus_tag="IV247_000817"
     CDS             complement(12327..13370)
                     /locus_tag="IV247_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8776908.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(13373..14041)
                     /locus_tag="IV247_000818"
     CDS             complement(13373..14041)
                     /locus_tag="IV247_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8776909.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            14183..16519
                     /locus_tag="IV247_000819"
     CDS             14183..16519
                     /locus_tag="IV247_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8776910.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(16634..16945)
                     /locus_tag="IV247_000820"
     CDS             complement(16634..16945)
                     /locus_tag="IV247_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8776911.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            17126..18601
                     /locus_tag="IV247_000821"
     CDS             17126..18601
                     /locus_tag="IV247_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAP8776912.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPTMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            18648..19232
                     /locus_tag="IV247_000822"
     CDS             18648..19232
                     /locus_tag="IV247_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAP8776913.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            19234..19653
                     /locus_tag="IV247_000823"
     CDS             19234..19653
                     /locus_tag="IV247_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAP8776914.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(19934..20218)
                     /locus_tag="IV247_000824"
     CDS             complement(19934..20218)
                     /locus_tag="IV247_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAP8776915.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(20570..21280)
                     /locus_tag="IV247_000825"
     CDS             complement(20570..21280)
                     /locus_tag="IV247_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAP8776916.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(21277..22749)
                     /locus_tag="IV247_000826"
     CDS             complement(21277..22749)
                     /locus_tag="IV247_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP8776917.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            22887..23360
                     /locus_tag="IV247_000827"
     CDS             22887..23360
                     /locus_tag="IV247_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8776918.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(23513..24064)
                     /locus_tag="IV247_000828"
     CDS             complement(23513..24064)
                     /locus_tag="IV247_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8776919.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            24169..24699
                     /locus_tag="IV247_000829"
     CDS             24169..24699
                     /locus_tag="IV247_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAP8776920.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(25182..25625)
                     /locus_tag="IV247_000830"
     CDS             complement(25182..25625)
                     /locus_tag="IV247_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8776921.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(25641..26801)
                     /locus_tag="IV247_000831"
     CDS             complement(25641..26801)
                     /locus_tag="IV247_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8776922.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            27308..29008
                     /locus_tag="IV247_000832"
     CDS             27308..29008
                     /locus_tag="IV247_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8776923.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(29110..29943)
                     /locus_tag="IV247_000833"
     CDS             complement(29110..29943)
                     /locus_tag="IV247_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAP8776924.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(30247..32412)
                     /locus_tag="IV247_000834"
     CDS             complement(30247..32412)
                     /locus_tag="IV247_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8776925.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            32672..34966
                     /locus_tag="IV247_000835"
     CDS             32672..34966
                     /locus_tag="IV247_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP8776926.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            34959..35624
                     /gene="pgmB"
                     /locus_tag="IV247_000836"
     CDS             34959..35624
                     /gene="pgmB"
                     /locus_tag="IV247_000836"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP8776927.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            35617..36624
                     /locus_tag="IV247_000837"
     CDS             35617..36624
                     /locus_tag="IV247_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP8776928.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            36646..37665
                     /locus_tag="IV247_000838"
     CDS             36646..37665
                     /locus_tag="IV247_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8776929.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(37946..39313)
                     /gene="nhaC"
                     /locus_tag="IV247_000839"
     CDS             complement(37946..39313)
                     /gene="nhaC"
                     /locus_tag="IV247_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP8776930.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(39422..40843)
                     /locus_tag="IV247_000840"
     CDS             complement(39422..40843)
                     /locus_tag="IV247_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8776931.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(41086..42963)
                     /gene="tdc"
                     /locus_tag="IV247_000841"
     CDS             complement(41086..42963)
                     /gene="tdc"
                     /locus_tag="IV247_000841"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP8776932.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(43254..44510)
                     /locus_tag="IV247_000842"
     CDS             complement(43254..44510)
                     /locus_tag="IV247_000842"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAP8776933.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            45090..46391
                     /locus_tag="IV247_000843"
     CDS             45090..46391
                     /locus_tag="IV247_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP8776934.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(46460..47059)
                     /locus_tag="IV247_000844"
     CDS             complement(46460..47059)
                     /locus_tag="IV247_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8776935.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT        14494 a         9739 c         8381 g        14605 t
ORIGIN      
        1 atccccactt gtttatcccc aaacaaaggt ggtctatgca ataaaatgaa cgtcaaagaa
       61 aaacgaatga atgctcgttg cttatttaca aattgtttta tttagaatac acagacagat
      121 ttatgaaaag aaaaagcttt tcgacacgat tatctgttcc aaaaaatgtc taatgaatgc
      181 tgaataagac agaaaatcaa tgaaaggaaa aggactagtt tattatgaaa aataaaaaaa
      241 cgatacgttt gctagctgct acttggctga ttgctccgtc tgttttgtcg accaataccg
      301 tatttgccgt agataatcaa atagctgatc aaacagtcac aacagatacg actacatcca
      361 gctcagctac tagtgaaaca acggattctt ttacagaaga cccaactgga acaactaact
      421 caacaacagg atcgaccgag ccaactgaaa ttgatccggc tgatccatca gaaaatgaaa
      481 agccagccga gacatattcc cttacaattg ctccttcttt gattggtaag atccaactga
      541 ctgcttcgac agcggattcc cctgaacagc aacctttgga tattacggaa agtggaacgg
      601 tagaaggttt attagcggaa acgaaggtaa cttacactat tacgccagct gaaggtgaac
      661 aaattgcttc agtcgctgtt aatggtgcca aagaagtaaa ttatgatgca aagaccttta
      721 cagtaggtac gaacaattta tctattactg cagaatttaa agctggaccg gatacttcaa
      781 cagagaaacc aactgatcct agtgaagaag tccctagtac accagatccg gaaacgccaa
      841 acaaaccaga tgtgggtaat aacaacaata ataatggttc aaataattca aacaataata
      901 acaataattc gaacagcggg aattcttcga atcaaacaaa cccatctgga tcgaataaca
      961 atggaaatac tcagagagaa ccaaaccatc caaacaatgg ttcatcgaat ggcggaacga
     1021 acaatgcttc atctcgtcca aatacagcaa atcaaagcgg tatagagaat ccatcttctg
     1081 cttcttctga ttttgttgta aaatcaccaa ttgatgctgt attgcctaca aatacaacga
     1141 atgtccaaca agcaatcgtt cgagaagcat tcaagcatct tggaaaacca tatgtatggg
     1201 gagcaaaagg tccaaatact tttgactgtt ctggtttgac ttattacgta tatatgaaag
     1261 caaccggtca ttatatcggt ggctggacag gcgaacagca atatgcaggt acgcagattc
     1321 cagtaagtca ggcccaacct ggcgacctcg tcttctgggg accttcttcc ggtgttactc
     1381 accatgtagg tatctatatt gggaacggac aatttatcca tgcgcctcaa ccaggagata
     1441 aagttcgagt aacttctatt tcagatttca cgcctgattt tgctgtccga gtaaacttag
     1501 caggtttacc tgctgcttca ggttcgctga caggcggctc atctatttta gatggattag
     1561 atggctcgtt ccatttcagt caaaaccaaa caactgatca gttcttgaaa aagattgcag
     1621 atgatgcaca ggaaatcggg caaaaagaag gaatctacgc atcagtcatg atggctcaag
     1681 ctatccttga aagtggatct ggaaacagct tattatcaag cgaaccaaat cataaccttt
     1741 ttggtatcaa aggatcatat aaaggaagca gcgtgacctt caatacactt gaacaagata
     1801 gttccggaca aagctaccaa atccgcgcac aattccgtaa atatccttct tacaaggaaa
     1861 gtttggaaga ctatgccgac ttgatcaaaa atggactgac tggcaatcct gatttctaca
     1921 aaccaacttg gaagtcggaa acaaaagact acaaagaagc aaccaaatat ttggaaggac
     1981 gttatgctac agatcgccaa tattcacaaa aattgaacgc gatcatcgaa gcgtacgacc
     2041 tgacaaaata tgatgaaccg aaaaaagaag cagcaacaac tgaagaagca aacgctgaaa
     2101 tcctcaatat gtctaagcga ttcgatgtgc caatcaaatg gacaacagat ggcgtgccaa
     2161 tcacaggatt agaattttca acgataaaac gcgtcaacaa cgcttcttcc atcttccgtg
     2221 tcgcttcaat ctgggatttg tggaacaatt ttacggcaag acagatacca gaaacaacta
     2281 ctcgtacagt taaagctgca actgttcctg tactaacctt gttaagttta gacaaatcat
     2341 tctggcttga tctgccttta aagaaataga ataaattaaa aaggggatgt gacactgtca
     2401 cactcccttt ttaatttatt ctatttcttt atatttattt tgtattccgt tacaaactcg
     2461 cgtacttggt taggttccag agtgacatct ccaaaacttt caagtcgctc cgctcctggc
     2521 agttcttgag tctcaaatgt gataccgcca tgatttacta atttattccc gcgcatttcc
     2581 ggtccatctt ctccaaaatt tgctgtaaaa atcacgattc ctgaagcatc tgtttttact
     2641 tggatcgata ctttttcatc tgggcttgtc aattccgcag catttagtaa accagattgt
     2701 tttttcaaca aaaatggatg atcgatgccg cccactatca ttgattggcg aaaatcactg
     2761 gcaaagacct cacctaattt tttaggacgt tggaagtcaa atgctgtgcc acttactgaa
     2821 actacttctc ctgtaggcgt tgtatcttta ttgagcgtag caaataatcg gctattcaac
     2881 caaagtatat gttcgtcgat tgactgtgct ggatcaccgg ttaaattgaa atagacatga
     2941 tttgtaggat tgaatactgt tttgcggtca ctgatccctt ggaccgtcac tcgccatgta
     3001 tcatcgttcg tcagcgtata ccgtacttct acatctaaag ttccaggaaa tccgttttcc
     3061 atatctgggc tagtctgata gaaaatgacc gatgcttcgt tttcaccatt gatgactgta
     3121 taatgccact gcttggcttc aaagctaggt gcaccaccat gtaaacaatg accagtgtcc
     3181 gggtctgttt ttacttcgaa cgtttcatca tctaatttga atgtgccatt cgtaatccgt
     3241 ccagccatac gtccgattga agcaccgata taagggtctt tttctagata ttcttttgct
     3301 tcatcaaaac ctaagatcaa ctctcgtttt tcattatcga ttattgtttg aaaatgaaca
     3361 acccgtgccc caagatcact gacagacaaa gatgtcccat tttgattcgt gatcgtaatc
     3421 aagtgatagt tttcgccaaa attttgttca ttgatttcca tgttccttgc accctctttt
     3481 ctttcatttg cactgattcc agcatacatg gaaaccgttt tcttaacagc ttattccgtg
     3541 agtaacgaga aaaaatttac tatattctaa taaaagaccg tgaaaaaaag gttgtgacaa
     3601 aaatattgtc acaacctctt tttccgaaca aacggtgttc aaaagcttgc atctgcggta
     3661 ataagcccaa ccaaacgaaa aatatgagga gctatttttg cttggctgtt cttattactt
     3721 gatgcaggac agctttttca caatctcttt ttcaatacga ttccagtata taaccatgtc
     3781 gtcagtacat agtagatgaa atacaatacg aagaatatcg taaacggaac ccaaatatct
     3841 ttgtatggat tttgcatgaa tagaccaaac atttgtaatc cgaataagac atggactaca
     3901 cctaagattc ctggtgctaa gaataacacg cctacttctc tacggatcga ttgtttaagc
     3961 aacccgcgtc gtgttcctat cttttcaagc atttcataac gagcaatgtc actcttgctt
     4021 cctgacaaga tcttgaacat caaacagcta gcaagcatgg tcaagaaagc taaacctagg
     4081 aagaatccca taaattcaaa accagagaat agaccattat acacttcata catttcatat
     4141 ttctggttgc tgtactctac tttttgcaaa gatggattgt tcattttatt ttgagcaaca
     4201 agtgtttcga ttggtttcag gtttgcttgg aagtctttta cctgaactag ctgtaaagtg
     4261 gattgtggca agttcagttg atcaaattct gattgactga ctaccttgat ttctttttct
     4321 tgctgttccg gcaattcata gcttcgaagt tcctcttgta cagtcagttc attcattttt
     4381 ttagcaggaa ctttttcttt cgtgatcttc ggcggtgttc catcttcatg ttttatcatg
     4441 atcaatggtt gagccgcaaa ttcacttgca ttatagtaaa tcgttcccgc atcttctttt
     4501 tgagtataag aagcagaaag tgttggtgaa agttctttga ttttttgctg atcttctgcc
     4561 cgaggattat tcatcactag atcatatcca gaattgtttt ctgtcaagtc tgcaatttca
     4621 tttttaaaac ctaatcctac cgtcaaagcg cccagtgcca aggcaaacag catagcgacc
     4681 attgagagca tttgtgtata ttcacgaata cgaaagttta actgtgacaa tgtgaaattg
     4741 ttcaattttt tcaatgaaat ctgttctgat ttctttagta atgataaaaa gaaaatgatc
     4801 acagaatgga aaatgaaata agttcctaat acgatcgtaa ctaaggctac ccctatacca
     4861 aagacttgat aggtccccac gtgtttcatc atatagtaac cgcagcctag aaaagcaatt
     4921 cccagtacta cttcaaggaa aaacaagaat ttatgctgct tgattcttgt tggtgtctga
     4981 tccgcacgaa tcaatgctaa aatcggtttt ttgacaatcg aaaaagcatt gacaacagca
     5041 gctaataaaa acaaaacagc aaaaaacaaa acagtgatca acaacccgtg aatactaaaa
     5101 ggtgtaaaat gcgaaatatt gagatctaac tgatccacta ataatcggtg gacgatactt
     5161 gtcaatccta caccgacagc tgaacccact aaagtagctg cgatccctac taagaatgtt
     5221 tcagaaaaaa tcatctgtgc gatttttctg ccttttgctc ccagcatcat aaacattgca
     5281 tagtcttttt gccgcatcgt catcaaaaag gaatttgcat ataaaatata aacgaatgtg
     5341 atgatcccta gtaaaacaga accgaatcgg aaaatgataa ctgtagccgc aacgatcgta
     5401 ttacttttca aaaaagcttc gttgcttgcc attgattcaa acatgtaaaa aatagcagat
     5461 gccataacta atcctgagaa taacaccaag tagtctctta aacggctctt gatcccagta
     5521 agtgataatt tccaaagcat agtcaatctc cccctactgt tccaagtttc caagaataga
     5581 tagtatttct cgatagaagg aatcacggct ttgatctcca cgtttcactt cttgatggat
     5641 accgccatcc ttgatgaata aaatacgttg gcaataactt gcagaaaacg gatcatgcgt
     5701 cacaagaaga atcgatacat gatcttttgt attcaattcg tccatcgtat ctaaaagatc
     5761 ccgggcactt ttggaatcca gcgcacctgt aggctcatcc cctaatagaa tcgttggttg
     5821 tgtgatcaat gcacgtgcgg cggcgacccg ttgtttttgt ccaccggaaa tctcagaagg
     5881 atagctttcc aaaatatgag agatggataa acgttctgca attttttgga cacgttgctt
     5941 gatttctttt ggtctgacgc cttgtaaaga aagaggaaca gcgatatttt cttgtgctgt
     6001 caagctttct aataaattga aatcttggaa aataaagcca atctcattcg cacgaaaatc
     6061 tgctagttga tttcctttta atgtggtcac atctttttga ttgattcgga tatgcccatc
     6121 tgttggttta tctaatgtgg ataaaatatt caaaagtgtc gacttaccag aaccagaagc
     6181 gcccatgatc ccaataaatt cgcctttttc tacatcgaag ctgatgccgc ttaatgcttg
     6241 cgtctttttc tcattgtttt ttccgtatac ttttgttaca ttctgtactt cgactatttt
     6301 gttcatttct ttccctaccc tttaattagt ttttccagtg ctgttttcgg cacgtctttt
     6361 ttttcgactt cttcatacga agtgacacct ttatttttgt tccaggtgat ttttaaatat
     6421 gctcctttac gaagcggtcg agtcaattgt cccttgaagt ccagttcttt ttccttaccc
     6481 tctttcgtat agccaggcag gatatatcta tagttgactg tttcttgtcc agagtctgat
     6541 tttgaaactg tttttgtacc atctgtcgta atttgtacat agtattcttc tccacccata
     6601 actacgtaat ctgcgatttt cacccctgca agtaccacaa cgatgaaaac aagtagaccg
     6661 atcagaagtt ttttcacttt ttccccttct ctcttacttt atatactgat tatagaaaaa
     6721 agcgaacaat tctgccttcg aatcaactat cattcttctt tcaattttgt cattttcttt
     6781 aaaacaacta aaaaaagagt tcaagacaat aatccgtcct aaactccttc ttcctatctt
     6841 acaaaaagta tgtcacttac ggtctttcat gtcgcctgta ttgataacag gttgcgttcc
     6901 tttgtctgta atgattgaga caagtaaatt attgatcaat tggactttcc gatcatcggt
     6961 gaaattgatt tcttgtccat cttcgatttg ctctaaggcc atttgagtga ttgtgacagc
     7021 tccttcaaca atggtttgtc gagcagacaa aatggctttt gcttgttgac gttggagcat
     7081 ggcactagcg atttctgttg cataggctaa atggttcaag cgtgtttcga ttacttcgac
     7141 acctgctact gctaaacgct cttgcagctc tttttgcaac tcgtcagaca ctgccgtcgt
     7201 gtttcctcga agcgtcaaat cattctcatt gaatgtatca tacgggtatt ggctagcgat
     7261 atgccgaatg gctgtctcac tttggatttc tacaaaatct tgataatacg ctacatcgaa
     7321 taatgccttt gctgtatcaa cgactttaaa gactactact gctgagattt cgatggggtt
     7381 tccatccaaa tcattgactt ttaaaacaga actattgaaa ttccgaactt tcaacgaaac
     7441 agtcattttt tgtgccaaag gtatcgtaag gaaaaagcca ttttctcgaa tcgtacctaa
     7501 atattgtcca aaaaacaaaa ttacttttgc ttgattagga ctgactacag ttgccgaact
     7561 taacaacaat aacgtgatca accaaagaaa aatacttaaa aaaagtgccc aaactttcgc
     7621 ttgccacata cctaaataga agaaaaacaa tccaactaaa gctaatacga ccaatccgac
     7681 aatccctgca taaccattga tataaaatgc tcttttttct ctcatgctac ttcctgcgct
     7741 cctttatcct tcaataaata tctaatgtta cttttttaat ttttttttcg aatacgcgcc
     7801 attacacctt ctagttctaa ctctttgtct tcagtgatgt accaaccgct tgcctgtgtc
     7861 cctccggctg cctgcggtaa ataatccaat tttgttcgaa cccagtgctt ttcttctgta
     7921 aaatagtctt ctaccaagac ctctagatcc atccctgctg aaagaggcaa atcttcccga
     7981 acagatttaa agataaatcg ttccttcttc ccatcatact tcaatattcc tgtatcgata
     8041 atcggtaaag aataataatc cagctctttc ttcaccgtca ataaatgttt gaaaacttca
     8101 tggtacactt gctctaattg gcgaccttct gaattgttcg ggtaattaac catcactttt
     8161 tctttgtctg gatcatagaa ggtttggctt agttgagtga tataagtatg taatctttcc
     8221 atttctgttt ttaacttttc tctttgacct tcttctacct tcatatactc tttcaccctg
     8281 ttttcttttt agatattttc tttcttattt tactatagtt atgtgcgtta agcgatgaaa
     8341 aacagcaaag attggaagga tctttgctgt tttttcttaa aatatccagt tgagtacagg
     8401 atcaatctgt ttcattgtcc tcttgatccg tcgtaatatt gatcgatgtg tctttagcag
     8461 tgatctttag ctttttctgt ccttgttcta acaaagtatt tgttattggc tggtagtctt
     8521 tttcaatata agattcacca gacgtttgga tagtatagcc actgttttcc atctcatgca
     8581 aagataaatt gccaccgttt aggatgactt ttccttcttt gctgatcaaa ctgtcttcta
     8641 gatggaagtt gctgttttca cctgtgacga tcaagttatc cacattggcc tcatctaatc
     8701 gaagtgagcc taattcatct ttcgcagtta ttgttcctcc tgtaaattca ttgaacgata
     8761 cccagccatc atgtgtctta gtattcactt ttttgattga tacttcgttg atatcaattg
     8821 cactattatt cccttcgatt gtcaattcag ataattcatg atctgcaggg acaacaatag
     8881 tgatttgtgc acttgtgtct tctaaactaa tgattgcacc tgttgatttt ttgactgcat
     8941 tcacagtcaa cacgccattt ttctccttta tttctggctt ttctgtctta tcataagtcg
     9001 tttttatctc aaaatccaat ccgcgtatga ccgaaactgt ttgattttct gcatggatga
     9061 cgattttgtt tgcatccttg attttttttg tctcagtaac catatcgatc aaccgaggac
     9121 cattatccca agtcagactc ttttgagcgc ctaatgtata agttgcacct gctaggaggc
     9181 caccagtaag catcagacca ataccggtaa tcaaaaaatg tttcaatctc aatttatgtg
     9241 tcctccttca aacgcccgct gaatgcagat ctctttttgt aataaaacgt cgtcccatcc
     9301 atttaacaaa gttcatcatg acataaaaaa gcccttttgt tcctttcagc aacagtggcg
     9361 aaataaatac tgcgcttcct gtggcgaaca gaccgacacc gataaagaat aatccactca
     9421 gaatcgattg tccaaacaca ctgattcctg agatgatcat gaataggcca acacctaaga
     9481 tactgataca taaaacatag atactaaaaa tgagcgctag taaagcaacc aatagtcccc
     9541 caataaccaa tataaacccc agtgctagtg gaatgaggac aggagaagca aataaaccga
     9601 taatgattaa ccaaatcagg cgaatttgtc tttttggatt tttcactaac tcgttttctg
     9661 cttgttcttc ttctgaaaag taactgattt ttaattttaa ggcaaactgc tttggtgtac
     9721 catattcatc aatgatctga tctaatgttt ttccagaatc taaaaaatat tcctcataat
     9781 actggatcaa atcaaaacga tcgtcttcag gaatttcttg aagatgatcc acaacttcat
     9841 tcaaatattc attcatttgt gctctcctcc aataaccggt ctaaagaatc tcgaaaaaca
     9901 atccattcat caataatttc ttctaaatga cgacttccca ttggtgtcaa cgagtaatat
     9961 cgacggtttc tgccttcaaa aggttcatca taggtctcaa gatatccttc ttttttcaat
    10021 cttctcaaaa ccggatatag tgtagattca ctgacagaga gactttcttg tacgattttt
    10081 gttaaggcat acccatacat atcttccttg cttaatgctg ctagcacagt accatcaaga
    10141 agaacagttg gaatctggat gtgcattacg tcaccttcta ttctcaagtg taatatttta
    10201 tttcaatcta tattatacaa catataatat agtttcaaaa tcagtttgtc aaatacaaaa
    10261 aaactgacta aaagatttga tcctttttag tcagcaaaaa atggcttctc tttgattttt
    10321 attataacag tggcataatc caatttgtcc acatccagct cagtggcatg ataacgatca
    10381 ttgcaggtat catgtcagct actgggaaca ttttcacgtt gatcattcta aaaccggtag
    10441 cgaccatcaa gaatccgccg caagctttaa aatctaagat catgtctggt gtagtcaatg
    10501 gataaataaa ttttgccaat aagaacaaaa tataaaaaat aatgaattgg ggaattgcta
    10561 tcacagaaac gacataccct aaattacacg caaaaatagc tgctgtgaaa aaatcaagga
    10621 cagatttaga aatcaaaatc gtactgtctc ctgtcatccc agaatctaaa cttccataga
    10681 tccccgtacc acttgcacaa aatagaacaa tgaccgtcac taatgtgctg ataaattcat
    10741 cgtgggtcat accaagcttt tcactaggga agattttaga aataggtact tgcatcagcg
    10801 cagccccctt atttatccca ttacctaaat gaacaatcaa accaatcgct gttcctatca
    10861 ctaaagcaaa aataacagcc ggcatatttt tcatcggcgc aatggaatag attcccatcc
    10921 ccatcgaaca cacaccaaaa accatcgtca gctgtgcttt aaaatgttca cttaatttat
    10981 ttcctactaa tccgccaatt agtccgccaa acaaaacgga acctacattg atcaaaactc
    11041 cagttggcat caaaaaactc ctctcttttt ctacgaagcg aaaaaaatca gaaaaatttc
    11101 tgtaattatt gctttcgctg caagaaagat tgtaacagac agagaagatt acaccaattc
    11161 attttttgtg ttaaagtatt ctttattgga ataagtagga ggaatatgat ggatatccat
    11221 aaattcgaag tttttcttga tttagccgaa acaatgaatt tcaccaaaac tgctgatcga
    11281 caattcacca cccaaggaaa tatttctaaa cagattcttt cgttagaaac agaattagac
    11341 gtcaaattat tcgaacgcgc ccatagaaaa atcgaattga ccgaagctgg aagcttattg
    11401 ctcccatatg taaaaaatgt cgtagaacaa taccatgcta tgcaagaagt gcttgaaacc
    11461 tatacgaaag acaacaatct ttcattaaat atcttaacga taccgacaat gatcaattat
    11521 aaaggttttt ccaaaatcac cgagtttttg aaacgccatc ctgaatttac agtccagttg
    11581 aaagaagtgg aaagtgtcga attaacttac aatgacgcca atgaacatag tgacacgatc
    11641 cactttgcta gaagtttcca ggccccacca agttcgattg agtggttgcc tactgaaaca
    11701 gacgattttg tagcagttct acctaaaaat catcctttag ctagtaaaaa gaaattggat
    11761 ctaagcgaac tgaaaaagga gaactttttg ctccttggcc ccactacgaa cttgtatcaa
    11821 cctgtactag atctgtgtcg cgaagctgga tttgaaccaa aaatctctta taaaggagca
    11881 agaatcgact tgatcatcaa tatgattgca aatgacatgg ggattgctat tgttatggaa
    11941 aaaacagtca agaatctatt aaaagaaaat actgtgattc tccctatttc tccaacaaca
    12001 gagagttatc ttgcatttac tcgtaaagtc ggtgagcatt ctttggcttc agatacattt
    12061 tgggagtatt taaatcaaga agaaaactaa gccctacatt ttctgtccat ctatccaccc
    12121 aaaaaaagag agcgacgcat gactttcgct ctctttcttg catcatgtcc ttgtttcaat
    12181 gacacctcta acatttctcg tagttcaata agaaagcagc gatttttatg ccaaaagaac
    12241 cgtaagaaca tttccctaat cacaaaaaaa cgactaaaaa cccctaaaaa ccttcaatag
    12301 aatttttagg ggttttattc tttttcctat tgtttactta accgtatcat tgctgtcgtt
    12361 cctactcctt cgatactgct ataatcaata cgtccatcat gcagttctag aattttttta
    12421 accagtgaaa gtcctagccc attcccagaa atttgacgac ttgtatctcc ttgataaaat
    12481 ttttcaaaca ttcgatctct ggtctcttca ttcattccta cacctgaatc ggtcacttcc
    12541 agtacaatct ctgtggctgt ctcaaatagt ttgatatgaa tctgtccatt gatttgattg
    12601 tactttatgg cgttatccat gatattcagc cacacttggt acaaaaactc ttcattgcct
    12661 gtgtaattta ctgcagcaag ttcgatatcc aattctagct gttctttctc ccatttaggc
    12721 tgaaaaaaaa cgattacttc tctgatttgt tcatctaatc gatactcttt cttttccagt
    12781 tggatacgct ggttttccag tttgttcaat tttaagatgt tctctgttaa ttgtgaaagc
    12841 tgcgtgatac tttcgataat ccgatataaa aatatttggc gttcttcgtc agagagattt
    12901 ggggcttgta acaattgaac atatccttgg attgttgcta gtggtgtttt gaattcatgg
    12961 gatacactgg acacaaagtc attcctcagt gtctcgatac tattgagttc ttggaccata
    13021 acgttgaaat ctttatacag ttgctgtact tctgctactt tctgctgttc atccattcga
    13081 atcgaaaaat ctcccgttgc taccagactc atatttgtcc ctagcgcact gattggatgc
    13141 aggatacgcc gcccaacaaa aatcgatatt cctgtaccca cgaccatact gaaccccgca
    13201 aagacgaata acgggtataa cgttggtttt ccagccgatt cagcaggcga gatagaatgt
    13261 cgagacaata agaaaataaa accgagaaaa cacagcagtg tcactaagat cgaaatgaag
    13321 acaatactag caaaatagat ccataattgt gaaaatagct tttttttcat ctttaaataa
    13381 ccgccttata gcctagtccc cgtactgtga tgatttgaaa atcttccgtc ttttcaaaac
    13441 gggtccttaa acgtttgata tgtacatcga tcgttcgttc ttctgtattc gtatctaatc
    13501 cccaaatatc atccatcaac tgttggcgag tgaagatctt gttgggataa gacagtaatt
    13561 tatacagaag cagaaattct ttttgaggca gaatcaacga atgctgatct tgtaacactg
    13621 tataagcatc tagctctaat tttgtttgtc cgattatcaa gctactgctg taattgatct
    13681 ttgaacgtcg aagcaatgct tccacacgta ggatcatctc attgacatcg atgggttttg
    13741 tcatataatc atctgctcct aatttgaacc cgcgtttctt atcctcaaaa tcggcctttg
    13801 ccgtaatcat tagaacaggt gtgtcaaatc tggtgctacg taatgagtct agcaagtcat
    13861 agccatccat tttcggcatc atgatatctg taatgattaa ttctatacgt gtcgcttcta
    13921 gttttttcag cgcatcctct ccattcaatg cggtgatgac tgaaaagccc gctcgttcca
    13981 acacagaacg gtaaagcaac tgtaattgcg gatcatcttc tactactaaa atacgattca
    14041 ttgatctccc tctgtttttt gaaaaatttc tagatttttt cttgtagttc atcttcagtt
    14101 aacatttatt tcgtatacta cgatcattgt tctgatatac ttattagtat accgaatatt
    14161 tcaatacggg agaagggtga ttatgcttca attaaaagat ataaaaaaat attataaagt
    14221 cggcgaaacc accaccaaag cattggatgg tgtgtctgtc gctttcagac aaaaagaatt
    14281 cgtagcgatt ttaggtccta gtggttctgg taaaacgact atgctgaatg tgattggtgg
    14341 actagataat tatgattccg gtgatatggt aatcaacgga aagtctacta aagactttaa
    14401 ggacagtgac tgggatgcct accgaaacaa ctctatcggt ttcatttttc aaagttacaa
    14461 cttgattggt catctaggca ttattgaaaa cgttgaactt gggatgacat taagtggtgt
    14521 ttctaaagac gaaaaacgaa aacgggcaga agatgcattg caccgagtcg gcttgacaaa
    14581 tcatatgcat aaaaagccaa atcagttatc tggtggacaa atgcagcgag tggccatcgc
    14641 tcgagcacta gccaatgacc cagatatcct tttgtgcgat gaaccaaccg gagcgttaga
    14701 tacagaaaca agtatccaga tcatggaatt gatccaagag ttatcgaaag aaaaattagt
    14761 gatcatggtc acacataatc ctgaactggc ccatcaatac gcagatcgaa tcattgaatt
    14821 ttctgatggt aagatcttaa cagattccca tcctcacatc gaacgaccaa aagatgatca
    14881 atttaactta cgcagaacaa agatgagttt ttggacagcg ttaaagcttt ccttcaacaa
    14941 tatccgaaca aaaaaaggac gtactttctt gacctccttt gcttccagta ttgggatcat
    15001 cggaatcgcg atcgttctgt ccctttcttc tggattccaa aaacaaatcg ataacacgca
    15061 agctgaaacg atggcgaaat ttccgattac tatttctaag gtaacgacta accagactcg
    15121 tgacgatgcc ggattgggtg caagcaaagc ggattatcca gacagcaaaa cgattactgc
    15181 aaaagtcagc gaagaagata aagcacagca cacgaataag attgatcaaa catacgtcga
    15241 ttacgtaaca gacatcgatc ctaacttaag taataatatc ggatttaccc gtactacagg
    15301 aatcaatctt ttacgtgatg taaatggcaa agtacagcct gtcagcttct cgaatcaaaa
    15361 tcctgatacg gaatctcttt ctttatctag tacgatgtca gcaatgacag gtgtgggagt
    15421 gtcaagtttc cctacacagc ttgacactag caaagaaaac tttttaaaag acaattattc
    15481 tttacttgcc ggaagttatc cagcctcagc aacagatgtc gtgctgatcg tagacggcaa
    15541 taataatact aatatcaacg cattgaagaa tcttggattt gatgtgaaag aagacgaaaa
    15601 acttgatttt gatgatatcg ttggtacaac attcaaatta gtaaacaaca atacttacta
    15661 tacaaaactt ccaactggga attttatccc taatacagat tatgatgcga tgtatcagaa
    15721 tgcttctgat gaattgaaga tctcggggat cctacgcgtg aaatcttctt ctacaatgaa
    15781 tctcttatct ccaggaatcg cttatagcga tcaattaacg actcaaatcg tcaacgaaaa
    15841 caaagaatca gaaatcgtca aagctcaaag agacagtggt gtaaatgtct taactactga
    15901 aaaagttgac gaaaatgcca aacaaacatt gcttagttat ttaggtggag acagcttgcc
    15961 ttcaagtatc atgatttatc cgaacaattt tgaagacaaa gaaaagatct tggattatct
    16021 agatgattac aataaaggca aatcagatga agataaaatc atttatactg atttagctgg
    16081 aacgatgact gaactgactg gcggattgat ggatgctatc acttatgtcc ttattgcttt
    16141 tgcagggatc tctttagtaa caagtatgat catgatcagt atcattactt atacatctgt
    16201 gattgaacgg acaaaagaaa tcggtgtact aaaagcttta ggtgcacgta aaaaagacat
    16261 cactcgcgta tttgacgcag aaacgtgtat tttaggtatt tcttctggga tattaggtgt
    16321 gtttattgca tggctagcta cgttccctat caatagcatc ttatacaaca tgacggatct
    16381 aaaaaatgtt gctcaactaa atcctgtcca tgcgattatt ctagtgatcg tctctactat
    16441 attgacgatg cttggcggtc atcttcctgc acgaatggca gcgaaaaaag acgccgctat
    16501 cgctttgcgt gcagaataag gttgtgacaa ccacatttag atttgagtca taagagcgct
    16561 gaaatagtag aaaacagcaa gaaacgagga agtaatatgt tccttgtttc gcgctgtttt
    16621 ttttaattat tttttataat tctggtatac ttttctttcc tttgtaccac aactcttctg
    16681 gcgtatacct gtcttttcca tttatgaata ctgttccatc tgcttcaatc gttacttcag
    16741 tcccaatcca catattacga gaaagtgcga gaagattttt tctttttcct cttccttttt
    16801 ttccaaattg caaggcatcg gttatctctt ctgtccaatg agatttcacg tattttactt
    16861 ctccgttttc aaatgtaact ttcaaaatat tcgctatatt ttccactttt actgctttag
    16921 gaaatgactc ctccattttt ttcactttcc ttcctccaac actaagtatg cgctttcttt
    16981 tagtgataag tatatcatag tttattctat tatcaaaaaa acgcatccat tccattgtag
    17041 aatactttac cgaacaagtt gaattgtttt cctctttttc accttttact atagataggt
    17101 aaagacaact aaggaggaat ctctcatgac agatcaaccg tatgatttaa gaaaagtcat
    17161 cgaagaacta aaaacacttc caggtcaata tcacgaaaca gatattgaaa tcgatcctga
    17221 agcagaatta tctggtgttt accgttatat cggtgctggc ggtacagtaa aacgtccgac
    17281 tcaagaagga ccaactatga ctttcaacaa tatcaaaggt ttccccggca cacgtgtgaa
    17341 catcggaaca atggctagtc gtaaacgtgt aggtgaaatt ttgcaccatg attatcgaac
    17401 acttggtcgt ttcttgaaag acgctgtaga aaatccaatc aaacctgtga aagtagacaa
    17461 agcagacgct ccagcacaag aagtcgttca tctagctacc gatgcagact tcgatattcg
    17521 caacattttg cctgcaccta caaatactcc tgaagatgct ggtccataca tcacaatggg
    17581 tgtagtctta ggatcagatc cagaaaaaac aatgagcgat gtaacgatcc atcgtatggt
    17641 tctagaagat aaagatacga tcggtatgta catcatgcct ggcggacgcc acattggtca
    17701 cttccaaaaa caattcgaag ccttgaataa accaatgcct atcactatca atattggttt
    17761 agatccagca attgccattg gcacgacttt cgaaccacct accactccac ttggctacaa
    17821 tgaactatgg tgtgcaggag ctttgcgtaa cgaacctatt caaatcgttg atggtatagc
    17881 agtcgatgaa gtgggtattg ctcgtgctga attcatcatt gaagcagaaa tcatgccaaa
    17941 tgaaacaatg caagaagata tcaacacgaa ctcaggaaaa gcaatgccag aatttcctgg
    18001 ctatactggg gatgctaatc cagctgtaca agtagtgaaa gtcaaagcag ttactcaccg
    18061 aaaagacaat ccaatcatgc aaacaacgat tggtcccagt gaagaacacg tatctatggc
    18121 cggtattcct acagaagcaa gtattcttgc cttggtcgat aaagctattc caggaaaagt
    18181 agtaaatgtc tacaatcctc ctgcaggtgg tggtaaattg atgaccatca tgcaaatccg
    18241 taaagaaaac gaagcggatg aaggtattca gcgtcaagca gctattcttg ctttatcagc
    18301 cttcaaagaa ttgaagacag tcattttagt cgatgaagat gttgatattt tcgacatgaa
    18361 cgatgtagtt tggactttca acactcgttt ccaaggagat aaagatatca tcgtgatccc
    18421 aggaatgagg aaccatccat tggacccatc agaattacca gaatataatc ctggctctat
    18481 tcgtttccgt gggataagtg caaaaactat ctttgacggt acagttcctt tcgatatgaa
    18541 agaacatttt gtacgtgcaa aattccaaga agtagaagat tgggaaaaat atttgaaata
    18601 atgaatgatc tgcaactgtt ttgacatcat aaaaaggagt gcggaaaatg gaacaattgg
    18661 cacaacggaa gaaaaaaatc gtgattggtg taagtggcgc ttcaggcacc atttatgcca
    18721 tcaacctagt gaagaaacta aaagaatatc cgatgataga aacacacgga atcatcagtc
    18781 catgggcaaa acagaactta aagctggaat ccgacttatc actaactgaa ttcgaaagcc
    18841 tgtttgatta ccattattca aacaaagatt taggtgctgc tgtcgccagc ggatcatttt
    18901 tgaccgatgg aatgatcatc gtaccggcaa gtatgaaaac agtcgcaggt atctctgttg
    18961 gtttaggcga caacctgatt gcacgtgctg ctgatgtaac aatgaaggag caacgaaagt
    19021 tagtcattgt tcctcgtgaa acgccattaa atgccattca cttggaaaac ttgactaaat
    19081 tagcacgact aggtgtgcaa atcataccac caattcctgc tttttataat cagcctcaaa
    19141 cgatccaaga tcttgttaat caccatacaa tgaaactttt ggatcaattt gaaattgtaa
    19201 atgagcattc aaaacgttgg cagggtgatt aatatggaaa aagaaacgat agttgaagta
    19261 accaaactag gtttcacgat ccaagctgct gttaaagtaa tcggtaaaga cttattgatc
    19321 actttaacag gcggagatac accacatatc ggaacagtca ctacattttc tcgtgataca
    19381 gagatgcaaa ctgtacgttt ccctagccac gatggtcggt tccataaaga cgatgtctta
    19441 gccgaaaaaa tagctgtgat catcaaaact gccgttccgg gaagttgtac gatcacttcc
    19501 ggtatccatg ttgaccatat ctccaatgca caaatcaacg cctcttttcc gatgtcagaa
    19561 gagttaggtt acaagatttt agattggttg gaaaaaatag atttccatat caaagaacca
    19621 gtttattata aagatggtga aaaaccatta tgaaagaaga ggttgtgaaa tcagcgttat
    19681 gacctgagta tgagagaaaa aaatggaaaa atagcttctc atattttgcc gtttttgagc
    19741 taataccgag ggacagctga ttgaacgccg tttatcaaga ggttgtgata aagccgcttc
    19801 gtttcgagta gtaagaaaaa ttttgtaaac atgggtttcc acatttttgc ataaatttga
    19861 cttactaccg agaaactggc ttttgaacac cgtttatcaa gaggttgtga cacgtctttt
    19921 gtcacaacct cttttatttt tgcttcactg taactgctac aatatcaccg atttcttttc
    19981 cgatgttttt acgaatatct tttcgtagtc caaggatgta gcagactgaa ccatctggat
    20041 ttttcacgcc catattcacg atactgcctt catatggcac attgtcaaaa gttgcttgga
    20101 ctttaactct tccagcaccg aattcttttc ttatatcata cggaaaaata atatatgccc
    20161 cgcccttgcc gacttcggaa gcatagattt ttgattcaaa agaataaatt tccctcattt
    20221 tatttctcct attttttaca tctagtatag catagcaaaa aaaagaagga actgtgacaa
    20281 aagtcttgtc gcagttcctt ttttcgaata aacggtgttc tcctgccaac tcctcggtca
    20341 caagtcacaa tagtcaacaa acatgagaag ctgttttttg aaattgctcc ttgtgactca
    20401 gagttaaacg gcaggctcac aacctcttcc ttaaacggtg ttcaaaagct tgcatctacg
    20461 gcactaagcc caatcagatg aaaaatatgg agtgctattt ttatctgatt gttcttactg
    20521 cttgatgcag agcagctttt tcacaacctc ctacatggtg ttcaaaatat tattcgcctt
    20581 ttgcttcttt cactaatttt gcgaatgctt gttgttggaa ttcagcagct aaggctttcg
    20641 tacgttgttc ttgctctgct ttgctcattt ttggattttc agtcattgct tttaccatac
    20701 cgtcttgaac ataagctttt gcttcacttt ccatcgtact ttgctgtttc ttcactaatt
    20761 cagctaactt ggctgcttgg tccgtactta attctaccca tgtctctggg attttgaatg
    20821 tattcactcg gctgtcatat tcgtgattgt tatcagcaat attgaaccag aatttataca
    20881 tgtcgttttt gtattcccaa taagtagttt tcgtcacttt ttcagcatcg ccgtctgtct
    20941 tttctacttt atttgtttcg ttatccgtac cagttgcttt tggtttttct tgtttttcat
    21001 ttgttcggta aagatacact ttttcttttc cattccctaa agctttatac aaaagcatat
    21061 ctgctccttg ggaatcagca ctggaaacaa ttttttctgt ttttgtttcc gttacttttt
    21121 ccattccaaa gtgattggac aaattagcga cgatcaaact tagtgaagcg acgaagatta
    21181 gtccgaaaat caaagataaa aaggtttgcc atgttttttt agcgaacacg ttcgttaaag
    21241 caaaagcaag caagctgaca atcaaaactc caataatcat gcattatccc ccgctttcat
    21301 tagaacaggt tcttttttct tcaataaaaa ggcaataata aagccaagaa tcccaaagat
    21361 gatcgctacg ataaacgcgg catggtaacc tgacagtgtg gcattgattg cctgatcttt
    21421 ataagctaat ggcgtattat ccaatacaga tttgcttggg atatttgctt ttgtcacatt
    21481 cgtcaacaca ctgatcaaaa tagctgtacc aatcgagctt gcgatttgac ggaatgtatt
    21541 atttaccgct gtcccatgac tgatcaagtt atttggcaat gcattcatac cagaagtcgt
    21601 taccggcatc atgaccatgg agatcccaaa catacggatc gcataaagga tcacgatata
    21661 taaaactgga gtcgtttttg tcaagaaagc aaatggtgca gttgccgctg tcaaaatgaa
    21721 catccctgtg atggctaaac gtttcgcgcc gtatttatcg aaaatcgcac ccgtcacagg
    21781 catcatagcc cccatgatca atgcacccgg caacaacatc aagccagaat ggaatgctga
    21841 ttctccacga atattttgaa tatacaatgg tacgaccatt tctgccccta ccatcgccat
    21901 atttgttaca ccagctaaaa cagctgcaat cgtgaatgta gatgatttga atacacgaag
    21961 ttccaagaat ggatgttcca aatgtaattg acgccatgag aacaaggcga tgaaaatcac
    22021 accaacaact aagaaaccaa ttactttcaa gcttccccag ccgtcatctc ctacactaga
    22081 aaatccgtaa agtaaactac cgaatccaat cgttgataaa atagcggata aaatatcaat
    22141 gctaggattt gacaatggaa taacactttt cattaggaag aatgctaaaa ccaagacaag
    22201 aacaacgatt ggcagtacca taccaaacaa gtcacgccaa tggtagttat caatgatata
    22261 accagataat gtagggccta aagcaggagc aagtccaata acgatccctg acatccccat
    22321 tgctgatcct cttttttctg gcgggaaaat agatagcatg atattttgca ataatggcat
    22381 cgatactccc acaccgcaag cttggatcaa acgaccgatt agtaatgtac taaaattagg
    22441 tgctgtaaag caagtaatcg ttccgatcaa aaagatcgtc atggcagaaa gatataaatt
    22501 tcttgaactg aatttattga tcagccatgc tgttattggg atcatgatcc cgttgactag
    22561 caagaaacca gtcgttaacc attgcacatc agacgctgaa atatcgaaag ctttcatcaa
    22621 tgtaggaaaa gctgtcgtca ataaggtctg attcaaaacg gtacaaaatg taccaattaa
    22681 taagacaaca actaaaagtg tccgattata tggttttccg taaatatcta cgggttggtt
    22741 gttactcata tttctccttc tttctgaatt ttttctgaaa ctactagaat actttagtcc
    22801 taaattcgct atttgtcaac ctacttgaca cataagttaa tacggatata attaagggaa
    22861 gcaagaggac aaaaggagtg acaaaaatgg ttggggcttc ccttaaacgt ttgttggaaa
    22921 atgatcaatt ggttgttggc atcagtgaat taagtgatat cgtaggtgtg tctccacgtc
    22981 aattaaggta ttgggaacaa aagggattca ttcgttcgat cgaagctgat gcgaattgtc
    23041 ctagaaaata tcgtttacct acagtcataa aagtagaact gatcaaaaaa tatttagatg
    23101 acggatttac tttgtcaaaa gctgcagaaa aggcagaaat ccgccaaaag aaaatgcacc
    23161 atgtccgtaa agttttttca aaagcaatca aagatatcga attgatcgac gaacgctata
    23221 cgattttaca aatcggtaac ttcaaagaag agaatgctaa gatgtacatc attcatgaca
    23281 acgagacaga cgaattaact tacaagctcc ttcctgtcga tgaaaaagct gactacgaac
    23341 agttgttaaa agaactctaa acgccactaa tctcctaagt accaagcttt ttaataagta
    23401 gatgctacat aaatacttga aaaaaggcct gtttgaacat aagaatatcc gaacggtgat
    23461 ctgcgtttct ttcatataga aaggaattct ccatcactgt tcggatattt ttttattttt
    23521 ttcggttact ttcttctaat acatctgtta gccaatttgc atattctgtt tcccgattga
    23581 tcgcatgcgc taaaatcagg tagtgtccgt aattttcatg tttttctttt tcagtaggaa
    23641 agatcgtttt ttttcgctct aatagatgaa gtcgtttttc ttcatgaagg tcacgctgct
    23701 gctgaataat ttctactaat gccggatcat tcacgtcttt gacgaaatac aatttcagaa
    23761 caaattcgtc tcgattaacc ggtagttcat tggtgggtgt atgaatccac tctgccaatt
    23821 gttccttccc ttcttctgtt aattcataaa ctttcttttc caatttttca cctgtgatct
    23881 ctacttgatg ctgaatgatt ccttgctctt ccatttttgc aagttcagga taaatctgac
    23941 tatgttttgc ttgccaaaat tctcctattt catttttgaa cgattgattc aattcatatc
    24001 ctgtcatctt cttcttactt aataacccta acaaaatata tcgaagtgta ttttttcttg
    24061 ccatcagttt tccctccttg acaaatcttt aaatccattc tatcataaat gagtaatcga
    24121 aaacatgtag tttattacat gttttaattt ataaggagat gcttaaacat gaaagaattt
    24181 aaagaattgg aagattttat tggaactcac tttatctata cgtatgacaa tggttgggaa
    24241 tatgaatggt atgccaaaaa tgaccatact gttgattacc gtatccatgg cggaatggtc
    24301 aaaggccgtt gggtaaaaga ccaagaagct gatatcgtta aattgacgga cggcgtattc
    24361 aaaatcactt ggacagagcc aacagggaca gacgtggcat tggactttat gccaaatgaa
    24421 aagaaacttc acggaatgat ctttttccct aaatgggttg aagaacatcc agaaatcact
    24481 gttactttcc aaaacgaaca tatcgcagaa atggaagcag ctcgtgaaaa atatgaaact
    24541 tatccaaaat tacttgttcc tgaatttgct acgatcactt atatgggtga tgcaggtata
    24601 gacaatgaag atgttattag cgaagaacca tacgatggaa tgccagatga tatccgtagc
    24661 ggaaaatact tcgatgaaaa ttataaacga gtaaaataag gttgtgaaag agaggttgtg
    24721 aaatcagcgt tctgacctga gtattagaga aagaaacgga aaaatagttc ctcatgtttt
    24781 gccgtttttt gagctaatac cgagggtcag ctgattgaac accgtttatc aagaggttgt
    24841 gaaatcagcg ttatgctttg agtaataaga acgctagtta gaaaatagct tttcatattt
    24901 tctaggctag tgggcttatt accgagaagc agccgtttga acaccgttta tcaagaggtt
    24961 gtgaaatcag cgttttgacc tgagtattag agaaacaaat ggaaaaatag ttcctcatat
    25021 tttgccgttt tttgagctaa taccgagggt cagctgattg aacaccgttt atcaaagatt
    25081 gtaacaactg catattcaca cagttaaatt agtgacttca aaatgaaata atgctcccag
    25141 caaaaaatta ggctgggagc attatttcgt tattctgttc atcaaaaatc agcgttttga
    25201 ttccatttca tcacaagttc ctcctcattt actgcttcat ctttttgctg tgtttgaatt
    25261 gtaaaacctt gtgaaagata gaaatgaata gctgattgat ttttttgata gactgtcaaa
    25321 gtcagtgttt tattggtttc ttttattttt ttcattagtt gcgtaccgat tctttgttgc
    25381 cggtattccc ttttcacaaa gattcctgcg ataaactgat caatcacccc agcaaaacca
    25441 ataatttctt gatctatttc tgctaagtaa atcactgcat ctggtaaagc tttcctaaca
    25501 gattctactt gattctccca ataagttgat ggaatgaatt catgagaatc caagttagca
    25561 tgcagccaaa tcgatagaac agattccagt tctttggcag acaattttgt tgttgtgcgg
    25621 atcatttttt ctctccttac ttattcattg attttaaaat aaaaaccatt tttttgaagc
    25681 tcttttacaa gttttcgccc ctcaaaaaag tatatcttct cttgttttac ttttctaata
    25741 ccaaaagaaa cagcaacttt aggatcatca gctgaaacga ttcgataagt tccaccaggc
    25801 aattgatcca gcttgacttt cccatgatgg tcgctttgta ctgaaaacgg cacaccattt
    25861 ctagtaagtg tttttttctt atgtttcaaa atgaatgaac taccatttac cggtttccct
    25921 gacgaatcac taataataaa tgaaaaatca agcacttttt tacttaatct atactgatag
    25981 attagtcgtc cgataaacac taacagtatg actacagcta ttatcgttcc tgcaagaata
    26041 aaccattgcc gcatggttct ttcctgattt ccttttcctt cttctttttt taccgattcg
    26101 ttataaggaa tccgatgtcc tgtcactagc aaacgatgcg tgttgatcat atacggcgta
    26161 catgtaagca aggtaactaa gtcttctcct tctgaaatcg ttagaacaga cgtgtcatct
    26221 ggcagtacca cttgtatatt tttaacttga taagccaatt tctcccccaa aacagtcaaa
    26281 ataaacgtgt ctccgttttc taaacgatct aattcggtaa ataaccgtcg attaggtaaa
    26341 ccgctgtgag cagagatcac tgaatgcgtc gaatttccac caagcgggaa agaggttcct
    26401 tgcaagacgc ctgctccatt ttcaaggatc ctaccattga gccgatcata aagtgaaata
    26461 tttaattgga tcttaggtat attgatactt ccgatcagcc attcctctaa ttggctggat
    26521 gttgtacttt catttctgtt cttttcattg aaaggatcag taccaggatt gaatccttgg
    26581 tcagttaatc gttggttttt ctcctccatt ttctttcgct gcacttcaat ttcagctgcg
    26641 gtccgttttt gtacttgttc gatacgtttt tgatcaataa aactatttaa agaatccaca
    26701 taaaatggat ataaagcgat cagtaaccct acgacgatac atattaataa aaacaaacga
    26761 aacaaaagtc gacgagactg tttttgctta ctctgcttca catgtgtcct cccttgcaag
    26821 tttttttgtt ctttctttta ttatatgggt tcaggaaaaa aatagaaaat gaaagccgat
    26881 gaggttgtga aaaagctgtc ctgcatcaag taataagtac agcccaaaaa aatagctact
    26941 catatttttt gttgggttgg gcttattacc gcagatgcaa gcttttgaac accgtttaag
    27001 gaagaggttg tgaaaactgc gttatgcttt gagtaataag agcgctagtc agaaaatagc
    27061 ttctcatatt ttctaggcta gtgagcttat taccgagaag cagctgattg aacaccgttt
    27121 accgagaggt catgaaaaag ctactttgtt tcgagtatta agaaaaattc tgtttcaaaa
    27181 caaggagatg caacaaaact tatgtcgcat cctctctcct tagaactttc acttatgttt
    27241 ttcttgtaaa attcataaat attttcatga attttctaat ttcttgccta cctctcgcat
    27301 aggatatatg atgaatttat ctaaagtttg caaaggagtt cgacaattga aaaaatatgg
    27361 cccattttta gcaatttttt tcattgcttt ggcatttctc atgcctcatt tattcacaca
    27421 taaactaatt ttgggtgctg atgccccatt ccattacaat cggttctatg atgccgcgca
    27481 acaaattaaa tatggggatt tccattattt tccttccctt tatggattcc agcaaagtgg
    27541 caggatcgtg aatgccgttt acggcccatt tttcgcttat ttacaaggat tgatcgtgtt
    27601 gcttgcaggt agctggtttc attatcagat tctttcaaat ttcattcttt atcttatttc
    27661 aggattttct atgctgattt tactgagata tgtcaatgtt cgaaattggg taagtgtacc
    27721 agtagccgtt ttattcatga gtacgtattc tattcaatac tggtctatca accaaggatt
    27781 cactagctgg gggacttgtt tttatccttt atgtatgatc cctattgctg atttcgtcct
    27841 tcataaaaag ttccctgtta tcaaagtcgc tgtctcagtg ggacttatga cacagatcca
    27901 tttactgtct tcgatcatgt tagctttgat gtatatccct ttttatcttt attacttttt
    27961 taaccagact gaaaaaggaa aagcactgct tcaactgatt aagtcggtct tgctttattt
    28021 tgttctgaca gcaaatattt gggcaggatt agctattgtt tacagtggga ataagattct
    28081 tcctcccttt gttaacagag aaatgagtca aaaggcgata gatctggatg gcagttattg
    28141 gctccattac cctaaggcct ttcctatatt gatattatca ggttttttgc tttttatttt
    28201 ctttcaacaa aagaacacct tattccaaaa aatcgtgttg tacacaagtc ttgttttctt
    28261 ttttctttct actagcttga ttccatggac gacattgatc gaaaaaaatg tgccttttat
    28321 tagtttgatc cagtttccat ttcgattttt cgtgccattt acgctattgc ttctacttta
    28381 ccttgccctt tcactgaacc agtggtctga acaaaagtgg ttccgtcttt taagtatatt
    28441 tggcattctt attttttcag cacagacgat ccagaactta taccagcatc tggaaaagtg
    28501 ggaaaatgaa acatttgtca gtcgacatac ttatctattc gatacgccgg aagaagcaag
    28561 gaaaacattc ttcagcaaag atatgtctac cagcttgttc gttttccaaa aaaccacacc
    28621 tgactatctg ccaatctata aggagacaaa agacaataaa tacgaccggt ataacgaaga
    28681 aatactagaa aacgaaacaa cttttataaa aacacatcca ggcggtcttt tgacaatcaa
    28741 gtggaaaaac actggggaca aaactgttca tatccctgtt atcgtctacg atcggactgt
    28801 gctacagcaa aatggaaaaa cattgactga ttatgaagta acagatatcg ggaccccaat
    28861 cgtgaagcag caaaaaggaa tcaacgagct cacgcttcat tatcaaacgc caatctattt
    28921 ctatttcatt ctttctctta ctttgattgg ctggtttacc ttgctatgtt tatttattta
    28981 tcatagatac aagcttttac gtgcttaggc aaaagggata aatacatccg atcaaaaaac
    29041 tctccaaacg gtataatcgt ttggagagtt ttattttaat cagttttttt atgttatatg
    29101 ttatattttt tattcgttca catctaattc gatttccacc ccgtagtgat cactgatgat
    29161 tggactatcg atcccatcaa aaatccgttg atatttttta acctgcattc cttcagggac
    29221 gaaaataaaa tctatacgca atttttctgt attttgttcc caaccatcta ttttcttttc
    29281 cacagtagct gcttttcctt tttcattcgc gacaacatac gaatcttgaa gaagaaggca
    29341 actttctctt acagtataat aaccttgaga gtctactgga ttattgaaat cacctaggaa
    29401 aaaaagtggt acgcgagttt ccaaaaagta gttttccaag tttttccact caaaataaaa
    29461 gccacttttt ttatctgtcc accaagaaaa gtggcagctc gagacaaaaa tgtcctgatc
    29521 atctatcttt gttttcccaa tcaagatctt gcgtgtctga taatcttctt tcttttgaaa
    29581 ttgggagacg gtaaggacct gactttccaa tgtttttttc gtaagcagtg catttccttc
    29641 ttcatattta tcataaccga tgtggctcat ttcccaactc caaaaatact cttttccgtg
    29701 ttcctttaaa agctgaacta aacggtaggc aaaattatct ttacggatag gtacttgatt
    29761 ctttaccgga cagaacttca ttagcttcgc aggttctact tcttcagatt ccagtaattg
    29821 gttgatttct tgtaaagcaa tcacttcata atcttctaac aggatcttat ctgctaattc
    29881 ttgcaatttt ttctctggtt ctggctcaag ccagctatgc gtgttgagtg tcatgaattt
    29941 cattggtttt ctccttgtct tcatttcgaa acaaaagggt tgtgacaaaa tttttgtcac
    30001 aacctctcca gcaaatagtg ttctcctgct aactcctcag taataagctc aatggaccag
    30061 gagctatttt ttgtttggct gtttttatca cttggtgcag ggcagctttt tcacgacctc
    30121 tccactaaac agtattcaaa ctgctgcttc tcggtaataa gcccaaccaa acgaaaaata
    30181 ggaggagcta tttttgtttg gctgttctta tgacttgatg caggacagct ttttcacaac
    30241 ctctccttac attttaatgg agccgatcac tttaccagct gtcactgtac ctaattgctt
    30301 gatcactact tcttctactt tatcgtttgt aaatacgatc atgatcgttg gatctttttc
    30361 ttcgttttgg atcacatcaa gatccatctc agctaatttc gttcctgttc ctacttcttg
    30421 gccttctttg acagcaatct caaaagctgg ctgattcatt tggactgtat cgattcccat
    30481 atggatcaaa acttcgatac catctttcgt ttccagccca attgcatgtt tgctagggaa
    30541 aatacttgtc acttttccag cgatcggcgc aacaacttct cttgatgttg gaataacagc
    30601 aaagccgtca cccatcattt tttgtgagaa taccggatct tttacttcag ataaaggaat
    30661 cacttctcca gaagctgcgg aaactagttc ttgttctata cctaagtatt gcttactacc
    30721 tgcttgtcca tttcctgtag aaaccatttc tggctctgga atctctgctc ctgattgcag
    30781 cagatcttcg atatctgatt tcaagacatc tgcttttggt ccataaactg cttggactcc
    30841 attgtctttg acgatcaatc ccatcgcccc tgctcgtttc caggcttctt cagtccctac
    30901 tttttctcga tcatttacac ttacgcgtaa ccgagtcata caagcatcga catctttgat
    30961 attttctttt cctcccagca aatgaatgat ctggaccact tgctgatctg ctgttcctgt
    31021 tccgctgtta gatacaacgg tttcttcact gtcattgtcg taatttccgt tacgtcccgg
    31081 tgtagcaaag ttgaattttt tgatcaagaa atttgctaag aaatacgtaa caacaccaaa
    31141 agcgatcaca cacaacacga agttgataag atctccacct agacccgctt tgatagctaa
    31201 cggtgtccga gtcagcaatt cgatattccc aaatgaatgg acccgcaatg gcaagatatc
    31261 tgccatcgca aatgcagccc cttggatcac tgaataaatg acatataatg gcacagcagc
    31321 aaacataaac atgaactcca atggttctgt tacacctgtt agaaagacag ccaacgctgc
    31381 tgaaaaatac attgatttat atcttgcttt cttatcagga tcgacattac gatacatcgc
    31441 aaaagccatt cccatcaaga tacctgaaga accaatcatt tgtcctactt tgaaacgagc
    31501 cggcgtccag ttatgcaaca caaactcata ttgcgacata tcccctgcac ctttaagatt
    31561 cactaaatca gtggcccaag ccaaccataa cggatcttgc ccaaatactt gtgtccccgc
    31621 ctgtgcgcct gataaaatct catacgatcc gccaagctgt gtatagttga taggaatcgt
    31681 cagcatatga tgcaatccaa atggcaatag taaacgttct aatgttccaa ataaaaacgg
    31741 tgctagaatc ggtgcgcttt cttgtgattc tgcaatccat aaaccaaaat tattgattcc
    31801 agcctgtacg tttggccaca ccacagcaag aatcaatgcc acgattgttg accataagat
    31861 aacaacaaac ggaacaaagc gctttccatt gaaaaatgac aaagcatccg gcaattttct
    31921 ataattatag tacttattat aagccattgc cccgacaaat ccagcgatga tccctacgaa
    31981 tactcccata ttcaaagctg gtgcttccaa tacacttgta aaaaatcctt taaccatgat
    32041 cttcgtaccg aacagagtgt gggtaaatgc atcttcattc gcaagcatgt cacttgtgac
    32101 accaaagata gccccagtga tccgattgat taaaataaat gagatcccag ctgcaaatgc
    32161 tccgcctgct ctttcttttg cccaacttcc tcctattgct aaagcaaata aaatatgaag
    32221 attaccaata attgcccaac caatattttc aattactccg cccgttgtga caagcggcgt
    32281 ccaatcagga ttgatcaagg gaattgattt ccctatactg atcatcaacc cagctgccgg
    32341 cataactgct accacaacca ttaaagcctt tccaaatttt tgccaaaact caaaactaaa
    32401 tatctttttc attccgttta gctcctttac gcaatcggtt tcgttaaacg tattatagcg
    32461 tgcgttttct tttttgtaaa tgctttattt tttatttttc tgtctatacc attctataaa
    32521 acctataata cgtgtgttcc cttctataat tttctcctaa aaatttattt ttttaagctt
    32581 ttttctatga aatattttct tttgtctatt gacttgtgca atcggttgcg tttattatga
    32641 acgtataaaa cagctcatta ggaggagaaa aatgaaacag atcaaacgac tatttgctat
    32701 tgatccatgg aaaatcagca cgacacaatt agataaaaaa aatcttcgac tgcaagaatc
    32761 tttaacaagt attggaaacg gttatatggg gatgcgcggg aactttgaag aaacttactc
    32821 aggggaccac catcaaggta cttatttggc gggtgtctgg tatcccgata agacacgtgt
    32881 cggctggtgg aaaaatggtt atccagaata ctttggaaaa gtgatcaacg ccttgaactt
    32941 tatcgcgata gatatttcga tcaacgatca gctgatcgac ttggcttctt tggaaccaga
    33001 agacttttac tgggaattag atatgaaaaa tggtgtatta tctcgtacat tcacaatcac
    33061 tacttctact aataaagtac gcatttcctt cgaacgtttt ctgagcattg tcaaaaaaga
    33121 atcagcttat atctatttaa ctatagaaat gcttgaaggt agcggaacag tcaaactcgt
    33181 atcaaaattg gatggaaatg ttcaaaacga agacagcaac tatgatgaac atttttggaa
    33241 tgagattgat cgtggaacta tagaagaaat cggctatttg acgactaaaa ctattccaaa
    33301 caactttggg attgaacaat ttactgttac tgcagcaatg cgccatttcg ttaatggaaa
    33361 atcagtcatc cctgactatc acgaagatac tttagcatta catggttgct tgtctgcaga
    33421 attatctcaa ggtgagacat ggtcagtgga aaaagaaatt atcgttctta ctagtagaga
    33481 tattccagaa gcgcagcaag cagaaaaagc tgtctcagaa ttaacgaacc agacaaacaa
    33541 ttatatccaa gcaaaagaag agcaaacagc ggcttgggca gaacgatgga agcttgccga
    33601 tgtggtcatc gaaggagacg aagaagcaca acaaggtatt cgattcaatt tgttccaact
    33661 attctctact tactacggcg aagatgaacg attgaatatc ggccccaaag gattcacagg
    33721 agaaaaatac ggcggagcca cttactggga tacagaagcg tatgctgtcc ctctttatct
    33781 tgctttagcc aaacctgaag taactaagaa tctgctgaaa tatcggcata atcaactgcc
    33841 gcaagcaatc cacaacgctc agcaacaagg attaaaaggc gcgctttatc ctatggtcac
    33901 cttcactggt gtcgaatgcc ataacgagtg ggaaatcaca ttcgaggaga ttcaccgaaa
    33961 tggcgcgatt gcctatgcaa tctacaatta tacgaattac accggcgata cggaatattt
    34021 gaaaaatgaa ggactggaag ttttagcaga aatcgcacgc ttctgggcag accgtgtcca
    34081 tttttcaaaa cgagcaaata aatacatgat ccacggtgtg accggcccaa acgaatatga
    34141 gaacaatatc aataacaatt ggtatacaaa tacgatcgca gcttgggtct tgcgttacac
    34201 gcgagaaaat tatttaactt atcaaaaaga agctgctatc actatcagcg aagaagaact
    34261 agccaaatgg aaagatatcg cagagaacat gtacttccca gttgacgaag aattaggaat
    34321 ctttgtccag catgacacat tcttagataa agatctcatg cctgtatccg atattcccac
    34381 aagcgatctt cctctgaatc agcattggtc ttgggacaaa atccttagat cttgcttcat
    34441 caagcaggca gatgtactac aggggattta tttcttcaat gatactttta caaaagaaga
    34501 aaagcaaaaa aactttgatt tctatgaacc aatgactgtc catgagtctt ccctttcgcc
    34561 aagtatccat tcgattttag cagcagaatt aggaatggaa agcaaagctg tagaaatgta
    34621 ccagcgaaca gctcgcttgg atttggataa ttacaataac gacacagaag acggcttgca
    34681 catcacttcc atgactggta gctggttgtc gattgtccaa ggatttgctc aaatgaaaac
    34741 tgcccatgat cagttgagat tcgcaccatt tctgccaaaa acttggaaag cttattcttt
    34801 ccatatcaat tatcgcggac gtttattgtt catctcagtc acagatgaac aagttacttt
    34861 cgagttatta gaaggttctc ctctagaaat ttacgtctat gaacagtctt attctttagc
    34921 ggatcgtcta gaaataacgc taagaaagga ggaagcaaat gtttaaaggg gtattgtttg
    34981 atttagacgg tgtcattacc gatactgctg aattccatta tcgtgcttgg cgaaagcttg
    35041 gtcaggaaat cggcattacg atcgaccaca cattcaacga acagctaaaa ggcgtcagcc
    35101 gagaagactc actctctttg cttttagctt atggcggaaa agaacactct ttttcaaagg
    35161 aagaatttgc agaattagcg aaaagaaaaa acgattatta tttagagatg atccagacga
    35221 tcgaaccaaa agatgtcttc cccggggtcg tgcctttact ggattcttta aaagaagaaa
    35281 aaatcaagat tgctcttgct tctgcaagta aaaacgggcc attcctactt gaaaaaatgg
    35341 gtcttacacc ttactttgat gctattgccg acccggcaga agcaacaaac ggaaaacctg
    35401 cgccagatat cttcctcctt gcagcaaaag ctgtcggact aaaagcagaa gaatgtatcg
    35461 ggatcgaaga cgcgcaagcc ggtatccaag ccatcctctc aagtggcgct caaccaattg
    35521 gcgtgggacg tgctgaagac cttggagagg agatcccgct tgtccctgat acgacttttc
    35581 ttacgatcga ttacttgaaa gaaaagtggc atgaccatgg ataatccaga gatctccgtc
    35641 gtaccagttc cagagactga aataaagaaa atcacattga agaacgacta cttagaagtc
    35701 gttcttctca attacggtgc ccgcctgcac caaatcttcg caccagacaa agaaggaaaa
    35761 tcagaaaatg ttttattgtc ttacgatcgt ttcgaagatg ttctaacaga caaaagcttt
    35821 tttggagcaa ctgtcggacc tgtagccgga aggatccggg atgcttcttg gggtgagcat
    35881 agattggaga aaaactgcgg atcacaccat atccacggtg gcacgaaagg ctggtctttc
    35941 caattttggg aagtagaagt tttcaaaact ccatcatcaa taggtgtggt cttttactta
    36001 aaagatgaat tttctaccta tccaggacca atcactgcta cgatcactta ccgtttaaca
    36061 aaaaacgaat tagagatgac aacctctgct agtagccttg ttgaaaccat ctgcaatccg
    36121 accaaccatg cctacttcaa cctttcagga aatggaacaa gagacatcta tgaacatcag
    36181 ttaacggtct ttttagatgg tgtgcttgaa ttagatcaag aaaaactgcc tactggaaat
    36241 tggacaaaaa aagaaaaact gccaatcgac tttagaaaat ccccaacact gcaagagatc
    36301 cttgcatgtt atccagatgg actagatgat gtatttcttt tgcatcatcc taggctttcc
    36361 aaaacttcct tacaattatt cgagaagcat tctggtcgcc aaatgactat agcaacttct
    36421 aataaaagta tggtattatt ttctacgact ggattcgaag ctgatttctc tgtcaatgga
    36481 aaacagatgc attcaaacta cggattagca atcgaaccgc aagaaatccc tgatatcgtt
    36541 catttcccca aatttggatc gatcaacctc catcctggtc aagaacggat cagtcagacg
    36601 atttatcggt tctctgcaca ataacaagag aaaggtgttt ttatcatggc tattaccgta
    36661 aaagatgtgg cgaaaaaagc cggtgttgcc acctcgaccg tctctcgtgt catcaacgat
    36721 catcctagta tttccgagac aacaaagaag aaagtccgaa aaattatgga tgaactaggt
    36781 tatgttccta acatgactgc ccgcaatcta ggaaaacgca tttccagtgc catcggcgta
    36841 atcttacccc ctcttgattc aaaagagcgg atcggtaacc cattttatct tgagatcatg
    36901 gaagcaatca acgaagaagc gcggaattat gatatgacta cagcaatcgc tactgctaaa
    36961 aattttgaat tgctgttaga gaacgtccag cgtatgcatt tacaaaaaca agtagatggt
    37021 tttattctag tgtattcaga tagtcaagat actgtcattg attatttata ccaaaaccac
    37081 atccctttca ctttgatcgg acagccttat catcatgaga acgaaatcgt ttatgtagat
    37141 aatgataacc agcttctagg taaacaagca acagaatttt tgatccagaa tggacataag
    37201 aatatcctgt ttgtgacaaa cacgactcat gaaagtttgt atttcgaacg atatttcggt
    37261 tatcaaaaag caatgatgat ggctggtctt cctgcgttat ccagtgtagt actagagcat
    37321 ccggaggatt atctgtcctt tgagaaaacg ttgaaagaaa acgaagccac agcattggtg
    37381 gtcattgacg atatcttcgc ccttcgcacg atgcagttgg cgcaaatgta tggctatgtc
    37441 attccagaga cactctccgt cattagcttc aacaactcga tcttctcaac attaacacac
    37501 ccttacctta caagtatcga tatcgatatc tccgaattag gacgtgtcgc tatgcaaaag
    37561 ctgcgcgaat tgatccatga agaattgagc agtggcgtac gtttggtcat ccctcatcgc
    37621 ttgatcaaaa gagagacagt cgttccttta gaaaacccat actaaatagc tgactcacta
    37681 aatacgctga ccatttatcc tatggtcagc tttttatgta aaagaagata ctacttctaa
    37741 cacattgttt atctgtaaaa aagagcgtgg gacaaaagta aagaacactt ttgtctcacg
    37801 ctctaaacac gtataaacgg cggaagcaga agcaactcct tcggaaataa gctgaaattc
    37861 acaaaaattt aaaaagcaat tttcttgagt ttcttcttat ttcttggagt taaacgcttc
    37921 tgtcccagcc tcttttttcc ttccttcaat tttttacacg tcgacgattc aataaaaaac
    37981 cgcctaatat tgtaaagaca ggtgcaagaa tcgagaaaaa ggcgaatggc aagtaatgca
    38041 acgcgctaac tttcatcgtt cccataatga atgtgccgct gactccccac ggtacgagag
    38101 aattgacggt cgcacctgaa tccgctagtg ttcgtgtcaa ataggttttt gataagccaa
    38161 cgtcatcaaa agaacgcttg aatgtttctc ctggaagaat gatggacaga tactgttctc
    38221 caacgatcag attgattccg atacagctca aggcagtaaa cccgactaaa cgagaaggtc
    38281 ggtccatctt ttcttttaat tcttgaacga tggtatcaac gatcagatat ttgatcagta
    38341 acccacctaa tgccaatgca agtaaaatca aactagcaga acctaacata ctcatgatcc
    38401 cgcctcgaga aagcagttca tctagctttg gatcaggtgt atttgcgaca taaccattca
    38461 tcaagatgtc tgctgctttt ccgaccgtca aacttttctc atggataaaa gaaagaacga
    38521 gagcagctgt cgatcccgct agtaatgtag ggatagccgg aatttttttg attgcccccc
    38581 caaataaaat aactagtgga atcaatgtcc aaggagagat ccaaaagctc gctttcatgt
    38641 tttggatcat ttcattgata ctagctagat cggctgaatg actgttatgt cctaatatcg
    38701 tataaaaaat caacgaaagg acgaatgctg ggaaatccgt ataacgcata ccagcgatat
    38761 gttcgaatag attaactcct ccaatacccg ctgccaagtt ggtcgtatct gataaaggcg
    38821 agcagttatt ccctaaaaag gcaccggaaa cgactgcgcc tgtagtcatt gcatcattga
    38881 atccaagtaa atgaccgatc cccaaaaaag caattccaat cgtcgaaatc gttgtaaatg
    38941 aactgccgac agtcactccg acaattgcac aaacaagaaa tgctgtcggt aaaaagaaac
    39001 gcacagaaac aagtttaaat ccataaacca taatcgtagg gatggttcca gataaaatcc
    39061 atgaagcaac taagacacca atcaaaagaa aaatcaggat tggaatgatc cctggagtga
    39121 ttcctttgat gatcccgtca tggatctcat cccatgtgaa ctttttaaat ctgccataaa
    39181 acaacaaaca catgaaagct gcaaggatcg ggatatgtgg cgagagctgg aatccgatga
    39241 tcagtactcc taggagtgcc aacaacacga tcaatacagc gatactttct tttaaagaaa
    39301 tatttttatt catcgtcgat ctcctatttc aaatgaaaag gccctgccat gaaaaaacta
    39361 ttcacttggc aggacctcct actaaatcag cgacaaaacg tcggctgaaa gacttatttt
    39421 tttatttttc ttctaaactt ttttctacct caatccccca agaatctttc ttgaagtgat
    39481 tgatgatcaa tggaataacg aacatgacca ctgtgatagc tgctactaat aatgcatcac
    39541 tcaatgtcgc tgtacctaat gtagcaaata cgacaacgat gatagagaag atcagcatgc
    39601 agctgactac ccaagctaat ccgtttccgt ttttaccaat tcggtaagga cgttcgaatt
    39661 ctggatgttt cttacgcatt ttaagcaatg aaacagcaat caaaatgtac acgatacaat
    39721 aaataacagt tgtagcattt gtaagtgtca agaatacagc acttacgtca tccattacac
    39781 cataaagcaa agcaaataat gaaataacga ttgattgtgt tagaacgaca tttctagata
    39841 cgccgtattt gttttcttta tggaatccga attttggtgg aaggaatcct tcacgagcta
    39901 cttggatgat tgtttttgat ggtccagtaa cccaagctga taattgtaaa agcacaccaa
    39961 taaataccat gaagctgaag atatttccga tcacttctgg taagcctagg atttgacagt
    40021 caatcaagat aggctgcgtg atatttgcta attccatttt tccatctggt acgacatttg
    40081 ctacaagcat cgcattgatg acattcaata aaactaatcc gatcaaagcg ataaatacgc
    40141 cttttgtata attttttgtt gcgtctttca aacgaggcat gtaaacagaa ctcatttcga
    40201 tcccaacaaa aataaatgtg ataccagcta agtatttcaa tgaatcaata tgttccaagt
    40261 cagggaaagc ttttgaccat gaaaagtcac ctagatagcc gtttgagact agtccgactt
    40321 tgaaggctgc caaaacacct aaaacgaaca tgatgactac tgggatatat acccctagcc
    40381 atacaccgat gttcccgccg acttttgcca tatcgaattt caagttcaag atcgtgatga
    40441 tccagtagat aactaaaata caacctaaaa cgaaccagtg attattgcca agttctacat
    40501 taccaaatgt gtttcctaac aatggtccta aagtagaagc gaccattacc atacctggga
    40561 acatttgcac ccataatagc catgcaacga caaatcccca tttgctgccc agtgctgttt
    40621 ttacccataa ctgaggaccg ccttcttgag gaagcatcgt ggaaagctca ccggaaacca
    40681 ttgatatcgg accggcaaaa aacaaaactg caaaaattga ataaaaaatc aatgtccaac
    40741 cgactgcggc taacgtagga atactccgaa ccgttgcaca cagtgccatt gtcagaccga
    40801 taaaggtccc aagacccatt ttacttactt cagatttact caccgttgac actctccttg
    40861 attttttgga taaaataacg gtgtctttga agtagtttca gcaataaacg aaacaattca
    40921 gcgaatattt gaggggattc tttgagttta ttgcttgaag ggtttcttca aagacaacct
    40981 caaaacaaag ggatcgaaga gcttaggtct ccccttgctc ttcagatcca aacatgcgtc
    41041 agaaacagtt agtattcaag ctgttttcta acatgattac cttgcttatt ttgcttcgct
    41101 tgccaataat tggtctgcgt agattttttc caatttttct tgaagagctg ctttgatttt
    41161 ttcagcatat tcttcaaagt gttcttcttt gttcatgtat ggtgtcatta cgctggcacg
    41221 caatacagtt acttttccgg cacggttcca ttcttcatct gagaatccta gttggttcac
    41281 aaattgtaat gggctgttgc catagtctgg gatagcaaag tcagtatgtg aagttaagaa
    41341 ctcattaccg taaatgcttc ctttcacgta tgaagaataa tcatatacgt catggttcaa
    41401 tttattcatt gcaactaagt catcgttgcc tttttctttg aatacatagt ctaccatatt
    41461 gaaatctgga taagttaatg gatgaacttc gatttctttg tctcctactt taaatgacaa
    41521 gtcattcaag aagttgtaga aacgatgtgc accttcgatt gatgcaccca ttaatttacc
    41581 atatcctgtg acattcaaag gtaagacatg gtgtgctgcc caaacgctgg ctgctgttgc
    41641 ccctgctttt gaaccttcca agatataagc cccaagtaat gctgggatat ctgcgccttt
    41701 ttcaaataca tatgtcgcaa agtaagagat aacgtctctc atgcggatat ctttgatgac
    41761 gataccaccg gcagaatatg gaacatagcc cattttatgt gggtcaatcg ttacagattc
    41821 tgcttcttcg attgctttat atgcactgtg tacttcttct aagatataat ctttgttttc
    41881 tgtaaagaca ttgtatttgt aatgaacatc ttttaaatct tcaaatggaa tgaagttgtt
    41941 gtcttcgtct aagaaaatcg cacggccgta tccgccataa gcagcatcta cgtgtaagta
    42001 gaagtaaata ccgtcttttt ctaatacgcg acgcaatgca acgattttat cgataccgtc
    42061 gattgctcct tcttctgttg atcctacaac accgactaca cctaggattg gtgttttttc
    42121 agctgctaga ccacgaacga ttttttcaag ttcgttgatg tccatgcgat agttgtggtc
    42181 aactggcact ggaatcactt ggtctaaacc gataccgatg atgtcggcag cttttagcca
    42241 tgaatagtgt ttagtttgtg gaaccaacca ttttcctaat ttttctaaat gttttccgct
    42301 acgtgctgag tgagctttga tttcgtcgat tttttctggg acgctgtcta acagattcat
    42361 gatttcttct gttgacaagt tcattaattc ccaatcgctt tttccagcaa ctagttcagg
    42421 tgtcacttct ttcattgcaa gcggcaatga tttgatgttg cgggcatacc aaagtccttc
    42481 taagtttgct aaagaaccat cagcaacgat gtggccccag ccatctttat agctcattaa
    42541 ttttgcaaat tccattccta cttcttcttc catttggctt gttgcaggag aagattcata
    42601 ggcaacgtta ttaccattcc atagcatagc aaaattgtac gctagtaaag atggcatcaa
    42661 tgtttcagag ttcatatgac cccagtaacg tccggcatta tgccaaggaa cggaatgggt
    42721 acgcatccgc gctgaaattt ctgatagaac atcctttgtt ttattcacag tgtgttcaaa
    42781 actagcactg cttttttctt ctggcgtaat aataggcatg tcttgaggca tgtagttttg
    42841 acgccagcct aaatgttcat ccaccaattc attcaacaaa gctttataga tttggccgtt
    42901 ctcagctttg tccccaatga atagggcatt taggtttaga tctttcgaca atgattcact
    42961 catagtgaaa ccctcctaaa aaaatattta aattttgtcg agccctacat tacacctgtt
    43021 cttctattct gtaaactaag ttttggcctg atttttaatc atattttaat atgtatcaat
    43081 tatccgaatt gttcacactt ttgaaataat taaaaaaaac gagggaattt acaaatgtaa
    43141 actccctcgc aaagattgta ttaacgacaa ccagcgttct attttaggat gggtttcaaa
    43201 attggaatgt tgtaatagta tttccctttt tcaaggtaaa aacttactaa attctaatca
    43261 attactttgg ccaaaaagta atttttctta ccttttcgaa taaccacaaa cttaccatcg
    43321 aattcatcgg atggatttat gacaaaattt aaatcggtaa cgcggtcacc gttaatactt
    43381 atagctccgt ttgaaacatc ttcacgtgct tgacgtttag atggttcaat ttttgtggaa
    43441 acaagcagtt ccacgatatt ttcaggtgtg cttgaaattt caacgttcgg cattttaccg
    43501 aatccttgcg cgatttcttc cgcatttagt tctttaatat ttccagagaa caatgcttgc
    43561 gttatttttt gcgcttcttt caagtcttct tcactatgca caaatcttgt aacttcttct
    43621 gccaaacggc gttgtgcttc acgtttctca ggttctgtct ctactttttt cgctaagtct
    43681 tcgatttctt cttgagacaa gaaagtaaag aatttcaagt atttcactac atcgcgatca
    43741 tcttgattca accagaattg gtagaactca aatggtgaag ttttcttcgg atcaagccaa
    43801 acggcaccgc ctgcagtttt tccgaatttc gttccgtcag ccttcagcat caatgggatc
    43861 gttaaaccga acgcttctgc ttctggtcct tctaattttc ggattaggtc taatccagct
    43921 gtgatatttc cccattggtc tgctccaccg atttgcaatt gcacatcgta tgttttatgc
    43981 agatgcaaaa agtcgataga ttgcaggatt tggtaagtaa actctgtaaa tgagattcct
    44041 acttccaaac ggctggcaac gatatctttt gctaacatcg tgttgatgtt gaagttttta
    44101 ccgtagtctc tcaaaaattc aagtaaagag atttttgaca accagtcgta gttgttcaca
    44161 aatgtgatat ttgcatcttt accaaacaat tttctcattt gattggaaag tgcgtccacg
    44221 ttatgttgca cttgttccat cgtttgcaac acacgttctg ttttgcggcc gcttgggtcg
    44281 ccgatcgttc ctgttccgcc gccaatcaga atatatggat gatggcctgc caattggaat
    44341 cttttcatca tcataaatgg aatcaaatgt ccgatatgca tcgaatcacc agttggatca
    44401 acgccgcaat aaagtgaaat gctcttttct tcaacgagct cgcgaagtct ttcttcgttt
    44461 gtttgctgat taatggcgtc gcgccaagtt aattcatcaa taatattcat tttactgacc
    44521 tccgtgtagt ttttttatga aagcaggaac ctgctagcat gtgtaattaa ttatttcttc
    44581 aggataggat aggaaataaa aaaagtccct tggcatataa gccaagggac gaaatctcgc
    44641 ggtaccaccc aatttgtatg tctagatatc caacatacct ctcgaacatt taacgctgtc
    44701 gcgtggcaat cactgccaaa gactcctgaa atgtaattcg ctttaaagca tgtactggtt
    44761 tgcagcgacc accagctttc tgaaacattg gctttaacaa ctacttcgtt tcgatcttcg
    44821 tcttgttatt attaatatgt gtctatcata gagcgcaact ttttctttgt caacagaaaa
    44881 aggatatttt ttttaaattc ctcatttttt cttttttgaa tccgctctaa ttccgaacat
    44941 tttattttca attattgtta aaagaaataa ctttttctgt attttttctg caatttatcg
    45001 agaagtcact ttactttgcc tcttattctt tcttcgttac aataaggaat gggacaagta
    45061 aaaaaatgaa aaagcaaggg acgaggtata tggaaaaaaa gcaaaaaatt attttaggag
    45121 ccacttctgc tttaactatc gttattttaa ttctaagttt gttttttgtc atccgatcat
    45181 ctacaaaaaa agaaccaatc attcctgaaa aaaaaataac agaaaaagca aaagaaccga
    45241 cagatgataa gcaacaacta gttactcaag caaaaaatct ggctcaaagc tacgattatg
    45301 accaagcgat tgcgttattg gaaaaagata gtaagaacgg atctactgaa acacaacagc
    45361 ttcttcaaac actaaaaaag gagaaagaac aactgatcga ctggtctgat cctactcaga
    45421 tctctcatgt ctttttccac agcttgatcg tggacccgca aaaagccttc cactctcaac
    45481 aggcacaagg atacaaagat tatatggtga ctgttgaaga attcaaccgt tcgatcgaac
    45541 aactttatca aaatgatttt gttttagtta atttgaatga tttgatccaa aaagacgaac
    45601 aaggaaatct ttcctttaac gggcttgcgc tacctgaagg aaaaaaacca ttggttcttt
    45661 cacaagatga tgtcagctat tatgaatata tggataattc cggttttcct agccgattgg
    45721 tcgtatcaaa taacaatgaa atcaaaaatg tctataaaga gaaagataaa gaagaaatcg
    45781 gtgactttga catggttcct ctgatcgatg cctttgtcaa gaaacatcct gatttttctt
    45841 atcgaggcgc aaaaggtacc ttggccctga caggttacaa cggagtttta ggttatcgaa
    45901 catcaaaatc tcaatatgga gacaatgaaa aaacaaaaaa agagattaaa gaagctaaaa
    45961 aagtggcgga acagttgaag aaagacggct ggagctttgc ttcccataca tggggtcact
    46021 tgaatatgac tcaagcttca ctagaagatg taaaaaaaga caatgaattg tggcagcaag
    46081 aagttgcacc tatcctagga aaaacaaata ttctgattta tccatttggt gcagacatca
    46141 gcgactggca gccttattca gcagaaaatc aaaaatttgc ttacttgaag agtcaaggtt
    46201 tcgatatttt ctgtaatgtc gatgcttcta caccagcttg gggacaatta ggtacggatt
    46261 tctatcgaaa tgctcggatc aatatcgatg gcattcgttt tcaatctgat ttagacggaa
    46321 aaaatcctat tcttgatcca ttcatcaatg tccatgatgt ctatgacaaa gaagcacgcg
    46381 aatctgcttg attttacgtc atttatgaac caaaaaagcc gaactgacat tttatcgtca
    46441 gtttcggctt ttttatgctt tatttcttct tcggtgcgat tcccttgatc aggacttctg
    46501 tccattctgc tgtatatgga agcagttttg cgtatatctc cggcttagaa attccttcta
    46561 ccattgcttg aaaatagagg atcaagaact catccgaata tttcaaatcc acttttcctt
    46621 ctttccgccc tcgatggaaa agatcaagca tgatactgaa actttcttgc gtatacgcat
    46681 ccatcgcgtc agaaaattct tttttttctt tttttgtata gtatgtcatc aaatcgatat
    46741 aaaattgttt gttcactcga ttgagataac ggattttgtt ttcactcatg gcaatcagtg
    46801 tttcttcaaa tggaagattt tccgcaatca acgctttcgc ttcttgccct aaatcattca
    46861 taaatgcaaa aaatacttca taaatcagcc cttccttgct gccaaaatat ttaaaaatcg
    46921 tcgctttact tacctgagcg ttctcagcta ccttatccat tgtcactcca gcggttccta
    46981 tgtctgtgtt catcaagtca aaagctgctt gtaaaatctc tttctttttc tcttgtgctc
    47041 tcttttcaaa accgttcatt tgtagagcct cccgtaattt taaactttta taatatatat
    47101 agttcaaatt tattctgagg tttattataa ctatctattg agaaagtatc aagctagcaa
    47161 ttccttgttt atgaattcat tgacagaaat ttataaaaag aatattatgt actaaaata
//
LOCUS       DACDIG010000014        46223 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.014, whole genome shotgun sequence.
ACCESSION   DACDIG010000014 DACDIG010000000
VERSION     DACDIG010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.014"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..119)
                     /locus_tag="IV247_000845"
     CDS             complement(<1..119)
                     /locus_tag="IV247_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP8776936.1"
                     /translation="MNKNDVLLIAKNKSLYFFPSIRSLDVWSGKDLLQSEFIKS"
     gene            401..1741
                     /locus_tag="IV247_000846"
     CDS             401..1741
                     /locus_tag="IV247_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8776937.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGIILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHAADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFAAYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLIIFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLTLRKGTLW
                     DKGISFFALAVLWLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1936..2379
                     /locus_tag="IV247_000847"
     CDS             1936..2379
                     /locus_tag="IV247_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAP8776938.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINAMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILTSKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(2641..4059)
                     /gene="nhaC"
                     /locus_tag="IV247_000848"
     CDS             complement(2641..4059)
                     /gene="nhaC"
                     /locus_tag="IV247_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP8776939.1"
                     /translation="MEKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKKYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDILEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPKLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(4463..5686)
                     /locus_tag="IV247_000849"
     CDS             complement(4463..5686)
                     /locus_tag="IV247_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAP8776940.1"
                     /translation="MTTTLSTNQLKDLIQYRLPEYQALALAIHDHPEVSNYEFYSSAA
                     LIKQLEKEGFWVEKEVAGHRTGFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDEIGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDAALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAALSTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSEKGLASIAIAAELLANTALDLLREPKLLEKIKQE
                     HQEIVARKKQEGATF"
     gene            complement(5718..6557)
                     /locus_tag="IV247_000850"
     CDS             complement(5718..6557)
                     /locus_tag="IV247_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP8776941.1"
                     /translation="MKKNIRFAVITFVAIIWLAGCSQGKAATTEKKETTVKLGIIGED
                     TDVWDDVKDRLKKENIHLEYVKFTDYNQPNAALADGSIDLNSFQHQFFLDNYNEEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENKKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDEHARPYVNIIVARKEDEDNKTYQKIVDAYQTEETAKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(6574..7254)
                     /locus_tag="IV247_000851"
     CDS             complement(6574..7254)
                     /locus_tag="IV247_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8776942.1"
                     /translation="MTEFFKTYLPNVYLIPDEFIEATKQTLYMSFWTAFIGGIIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILVLVFLSQFISN
                     QLIKKVTH"
     gene            complement(7256..8329)
                     /locus_tag="IV247_000852"
     CDS             complement(7256..8329)
                     /locus_tag="IV247_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8776943.1"
                     /translation="MIELRDISVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEAGK
                     IIEKGSSIQIFSHPEEELTKDFIRTATHLDQALETISAHAAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRQKALDYLIKS
                     GVKVTILQKNELDNRIKVIEGGN"
     gene            complement(8979..9650)
                     /locus_tag="IV247_000853"
     CDS             complement(8979..9650)
                     /locus_tag="IV247_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8776944.1"
                     /translation="MKGAIFDLDGLLVDTERTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVQAAGSPEKVQEVRAKREEYFYQQLTSGAIQLKPYAKEILTDLKERHM
                     KLGIATTTVTKRATDILDHFQLADYFDTFTFGDEVSENKPSPVPYLTALERTGLEALS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYSVEEKEKLNLLAEGKDLRTVIEMLD
                     KKTEK"
     gene            complement(9738..12416)
                     /gene="mgtA"
                     /locus_tag="IV247_000854"
     CDS             complement(9738..12416)
                     /gene="mgtA"
                     /locus_tag="IV247_000854"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAP8776945.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASPNGLS
                     EEQIERSRETYGENAISYGKKTPFIIEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGEYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNDTLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEAAETLDTDTINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVAPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELITGEKISEMNDHELAQVAEEHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGKFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQQVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKIPFIQSNATFAMLVITTLGIIVGSVLPFTGFGAELGLM
                     PLPGNYWLWLFITVVAYLALVTAVKKIYIRRFQELL"
     gene            13076..13513
                     /gene="ndk"
                     /locus_tag="IV247_000855"
     CDS             13076..13513
                     /gene="ndk"
                     /locus_tag="IV247_000855"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAP8776946.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFATMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKGADAVPGTIR
                     GDYALPGTENIIHASDSRDAATKEIARFFPPFDTNKPSEKAAK"
     gene            complement(13535..14401)
                     /locus_tag="IV247_000856"
     CDS             complement(13535..14401)
                     /locus_tag="IV247_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8776947.1"
                     /translation="MYDGELIKKLRVNKKLTQTQLAEGICSKTSLVGIETNSVKKVSF
                     GTLKSFLERMNMTLAEYEWIRNQLDEPKKLKKKRRMLDRIQEDSFDPYKEIANNRRHY
                     KKTEDLYYLVLNLHMSKKTEVSETEFEFLRYECRIIEEYLMQVPEYGQFEVSILADFP
                     WIFSDRFIENNYLKIKKRMRRIAQLNNHEQYLFTFLTNLTLFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKSENHQEFFAVIEYTLGKNQRNSL
                     EKKLLAIAKPSS"
     gene            complement(14635..16713)
                     /locus_tag="IV247_000857"
     CDS             complement(14635..16713)
                     /locus_tag="IV247_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP8776948.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVSKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPATLGQTEGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTIL
                     YPVTGTNAEQ"
     gene            17206..18423
                     /locus_tag="IV247_000858"
     CDS             17206..18423
                     /locus_tag="IV247_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP8776949.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEENTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETREKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEASPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
     gene            complement(18882..19505)
                     /locus_tag="IV247_000859"
     CDS             complement(18882..19505)
                     /locus_tag="IV247_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8776950.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWEEYKLTANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLVVLLLTHDGDKVARLKNGKVVTAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     ASSQKNESTELSPSDSGSGQTITGNEENQASIITNESSEGQYQTYGQ"
     gene            complement(19661..21058)
                     /locus_tag="IV247_000860"
     CDS             complement(19661..21058)
                     /locus_tag="IV247_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8776951.1"
                     /translation="MKRYQFPKNFWWGSAASGPQTEGRISGDGKGENIFDYWYKNEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HHYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFQLFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKAYHEMDQSGVI
                     GIILNLTPSYPRDESNPEDVKAARIADAFFNRSFLDPAVKGEFPSELIERIKELDLMP
                     EIHPDDLTIIKENTVDLLGINYYQPRRIKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEKGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDRNGFEA"
     gene            complement(21080..22051)
                     /locus_tag="IV247_000861"
     CDS             complement(21080..22051)
                     /locus_tag="IV247_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8776952.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMEKISEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISNRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHAIDGLIVFLSEEEWSGFEMLTKMSIPVVALNCPGGPAGIDTIVVNDR
                     ESVAKVIAHHGGQGHKTIGFIAAAQTDYVARERLAGAKEAVQKDPSIHLEIFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            complement(22058..24358)
                     /locus_tag="IV247_000862"
     CDS             complement(22058..24358)
                     /locus_tag="IV247_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 3 glycosyl hydrolase"
                     /protein_id="HAP8776953.1"
                     /translation="MYDYPYQNPKLTIRQRVEDLLSRMNIAEKVGQVNQHLYGWECYE
                     KNGDKIELTEKLKEHVKWGGGLGALYGLFRSDPWSKTDYKNGIPAKESWKVANLVQEY
                     VLQHSRWKIPVLLVEECPHGHQGLDGISYPTNIGRGNTFNVALLEQSSRLMAKELAAK
                     GVHLALVSTLDLAKDPRWGRTEECFGEDPILSARFSEAVVRGFQGEIISEKVSFLDQT
                     VEEINKKKDQIGVVLKHCIAQGEALGGHNSGTVTIGRREFSDIYGPLLKSTRNAVGVM
                     AAYNDIDGIPCHINRALFEQTLRKEIGYQGIVMADGVALDRLSDVFTDKKTAAAYALA
                     AGIDLSLWDETYTKIAEAIDNQVVDEKLLDQAVRRVLSVKFLLGLFEQPFANDPKEYW
                     DHLMEESEHLNLETAKESITLLKNDGILPLDQQVKNIAVIGPNAHDVYHLLGDYSAPQ
                     TEDRLKKKIVQEIKTEFPQSKVSYAQGCEVRNQEDQEEKLLEAVTLAQNSDVIIAVLG
                     GSSARNFDMEFLRNGAVSSKGINMDSGENVDVASLSLGGYQEQLIEQLYQLGKPIITI
                     LVQGRPYDLMKIESFTNAIATAWFPGQEGGKAIAQMISGRNNPSGRLSLSYPRNSRQL
                     PVYYYQRAASKQENYYDLSGSPFYPFGHGLSYTTFEYSQMEVKTDIDKVIISIKVKNT
                     GSVSGKTSTLVYVRLIDGVVIQRTKLLKAFRKDELRSQEERLLVFELTSEDFSYMDID
                     DKRHYAKKAVIMVEQQEKEIKCNWQE"
     gene            complement(24360..25685)
                     /locus_tag="IV247_000863"
     CDS             complement(24360..25685)
                     /locus_tag="IV247_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8776954.1"
                     /translation="MKVVDRLSEKMLPIVSKMSSQRHLLAIRDSFIVTMPLVMAASVM
                     VLLNALIFSNATVQRILDLSPLAELASIVNNGTMSILAIIVCYNIGWNLATHYIQTGV
                     IDDPSFSPVHAGILSTAVMFILMSLSSTVTLMDGNTAEATGVFLQSLTSSSGLATAMI
                     AALVSTELFIKLAKMKSFKIRMPEGVPPAVAASFNSLVPEIVVILFFSVVVFTLNHTT
                     GLNVPQLIELAIQTPLKAFVLSVPGILFLQFFSDFLWVFGMHGSSILAPIRQAPLLQS
                     IQENMDAFSVGKEIPNIITEPFTNAFGLIGGGGCILPLVIAILWASKRQEQRSIAKFG
                     LTTCLFNITEPIMFGLPVVMNPIYMIPCAIIPSINLVIAYAATSLGIISKTVAAAPWI
                     TPPVIQSFIATGGDIRAAVLTVILIILDVFLFLPFVLAANKAKLAEGGY"
     gene            25916..26341
                     /locus_tag="IV247_000864"
     CDS             25916..26341
                     /locus_tag="IV247_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8776955.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DITNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGADSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKEATVTFTKIGNYRLKDKDGNIYSL"
     gene            complement(26839..27540)
                     /locus_tag="IV247_000865"
     CDS             complement(26839..27540)
                     /locus_tag="IV247_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP8776956.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDTFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(27556..27996)
                     /locus_tag="IV247_000866"
     CDS             complement(27556..27996)
                     /locus_tag="IV247_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HAP8776957.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVLAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFEKLPVETYVGEYL"
     gene            complement(27996..28571)
                     /locus_tag="IV247_000867"
     CDS             complement(27996..28571)
                     /locus_tag="IV247_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAP8776958.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(28982..29488)
                     /locus_tag="IV247_000868"
     CDS             complement(28982..29488)
                     /locus_tag="IV247_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HAP8776959.1"
                     /translation="MDYKKRVQQMFLDSGVVLKETELERIEYTDFGLGAIETEGLNLF
                     VYENNERYCGKELVLLPYQTCPEHRHPPRDENEGKKETFRCRKGLVYLYVEGKETKKL
                     SCIIPKGQERYYTAKKEIILHAGDQYTIEQNIKHWFQAGATGAVITEFSSSSDDPSDS
                     FTNPAIYR"
     gene            complement(29499..29978)
                     /locus_tag="IV247_000869"
                     /pseudo
     CDS             complement(29499..29978)
                     /locus_tag="IV247_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(30150..30851)
                     /locus_tag="IV247_000870"
     CDS             complement(30150..30851)
                     /locus_tag="IV247_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8776960.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQEILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(30959..32401)
                     /locus_tag="IV247_000871"
     CDS             complement(30959..32401)
                     /locus_tag="IV247_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8776961.1"
                     /translation="MQSRFSEKFLWGGATAANQLEGAYDRDGKGLSVADAMPGGKQRF
                     AIIGSEEFDWTIDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPN
                     GDEGTPNEAGLEFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYE
                     RFAQTVLERYSSKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVA
                     SSKAVKIGHELRSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGE
                     YPAYTARTHKKYGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSE
                     GNLLGGVKNPFLEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDG
                     TIIDDYRIDYLRRHIEAMANAVADGVDLMGYTPWGCMDLVSASTGEMSKRYGFIYVDL
                     DDEGNGTLNRSKKKSFDWYKEVIASNGVNL"
     gene            complement(32415..34280)
                     /locus_tag="IV247_000872"
     CDS             complement(32415..34280)
                     /locus_tag="IV247_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8776962.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(34401..35252)
                     /locus_tag="IV247_000873"
     CDS             complement(34401..35252)
                     /locus_tag="IV247_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8776963.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            complement(35562..36275)
                     /locus_tag="IV247_000874"
     CDS             complement(35562..36275)
                     /locus_tag="IV247_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP8776964.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(36343..36669)
                     /locus_tag="IV247_000875"
     CDS             complement(36343..36669)
                     /locus_tag="IV247_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP8776965.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(36792..37019)
                     /locus_tag="IV247_000876"
     CDS             complement(36792..37019)
                     /locus_tag="IV247_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP8776966.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(37341..38594)
                     /locus_tag="IV247_000877"
     CDS             complement(37341..38594)
                     /locus_tag="IV247_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP8776967.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLIGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            38655..39395
                     /locus_tag="IV247_000878"
     CDS             38655..39395
                     /locus_tag="IV247_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8776968.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(39481..40239)
                     /locus_tag="IV247_000879"
     CDS             complement(39481..40239)
                     /locus_tag="IV247_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP8776969.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(40236..41264)
                     /locus_tag="IV247_000880"
                     /pseudo
     CDS             complement(40236..41264)
                     /locus_tag="IV247_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
     gene            complement(41284..44451)
                     /locus_tag="IV247_000881"
     CDS             complement(41284..44451)
                     /locus_tag="IV247_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8776970.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            complement(44675..45559)
                     /locus_tag="IV247_000882"
     CDS             complement(44675..45559)
                     /locus_tag="IV247_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP8776971.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(45654..46091)
                     /locus_tag="IV247_000883"
     CDS             complement(45654..46091)
                     /locus_tag="IV247_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8776972.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
BASE COUNT        13330 a         9801 c         7863 g        15229 t
ORIGIN      
        1 gatttaatga attccgactg tagtaaatct tttccggacc aaacatctaa agagcggatg
       61 gaaggaaaaa aataaagtga tttatttttc gcaattagca atacgtcgtt tttgttcatc
      121 tgttccgcct cactttctct gtcacttttt cctatgatag cctgtttaaa tagtaaaaag
      181 ccagtaaaac gcttcctatt atttttataa aaatttaaca tttctatgaa aatacgatga
      241 aataaatcat tcttccatta aaatatgccc aaaagttttc tgaatagttg taattatctg
      301 aaaatttgtt atcattagtc attattatta ttactattta ttcttactat ttattcttac
      361 tatgcatttt gattgtgaat ttaaaagaaa gaggttattt atggaagata aaaaattgaa
      421 aaaacaactt agctcccgac atatcaccat gctagcttta ggtggagcga ttggtgcagg
      481 tctatttaaa ggaagtggcg aagcgattgg aatcgccggc ccttctgtat tgatcgcttt
      541 tttgattggc ggcataattt tatatatcgt gatgaaaggt ctgggaaaaa ttgttttaag
      601 tggtggtgat acgcaccacg gattatctgg attgattcgt ccatatctag gtgctcatgc
      661 tgccgatttt accgattggg tctactggtc catgtggatt atcaatatca ttgctgaagc
      721 tgttgcagct gcttcctttt tacagttgtg gttcccccac atcccagctt gggtatttgt
      781 ttttatgttg gctgttttaa ctactatcat caatctttat tcggtacgat tattcgcaga
      841 gacagaatat tggctggcct ttgctaaaat cagtgtcatt atcctattga tcatttttgc
      901 tgcttatctt gtcggtcagc aaatgttggg tactggtgtt ttccctaccc ttcaacaatt
      961 aactgatcat ggtggtttta ctccccacgg tatgaaggga atcatcagtt ctttgcttgt
     1021 agttatctat tcttacggcg gttctgagtt gattgctatc acagtaagcg aagcagatga
     1081 tcctaaacgt gcgattccta aagcaatcaa aggcgtcatg ggaagaatca tttcctttta
     1141 cattattcct ttatttttgc ttttgattat ttttccttgg aatactttag caggcacgaa
     1201 cgttagtcct tttgtgatgg tatttgaaaa aatgaatatt ccatttgcgg cagatatcgt
     1261 caatttcgtg attgttcttg ctctgttctc gtctatcaat tcaggtgtat atgcttcttc
     1321 gagaattttg ttcttccgct tgaaagatcg gaaaggttcc tctagaaaac tagctgtttt
     1381 aaacaagcat caagtaccgc aacgtgctgt gctgttttgt accagtgttc tttatctagg
     1441 tgtggctctt tcttattttg tcggtgacaa attatttggc tatctggcag gttctctttc
     1501 ctatacagta ttattgatct ggatcattat cagtgctgcg tcctttgtac tgacactaag
     1561 gaaaggaacg ttatgggaca aaggaatcag cttttttgca ttagcagtct tatggttgat
     1621 tttccttggt atcctattca cgaactccat tggggtaact gtattgaccg gtttgcttta
     1681 cctgttcatt tatttcagtt atcaaaaaaa gaatgatgct ttcgtcttga cgaatgaatg
     1741 aaaccaatag agaattttat tctatttata ttgaatatct ggagctattg aaaaaaagga
     1801 gctgcgacaa aaattgttgc agttcttttt tccgaataaa cggtgttcaa aaaccatctg
     1861 tgcagattaa ttgattgttc tccacgctca tgacatgata gaagaaagga atgaagaaaa
     1921 aggagtttgt tttaaatgaa aaagtattgg aagtttttgt tgatgattgg tgtttcgacg
     1981 gtagtcatgt ttggtatgat gtatttgaat gtttatgcgc ttgatcatat cttcttcagt
     2041 cgcacacgtg tattcatggc gttgatgatg ggtgcagtca tggcagtcat catgttgttg
     2101 ttcatgtgga aaatgtatga aaacaaaaag ataaatgcta tgattttagc cgctagtctt
     2161 cttgtttttg ccggttcctt atttatggta cgaagccaaa caactgtcaa cgatacggcg
     2221 tggatgaagg cgatgatccc tcatcactct attgctatct tgacaagtaa acgagctgat
     2281 ttaaaagatc ctgaagtaaa aaaattagcg gaagaaatca ttcaggcaca agaagaagaa
     2341 atcgacaaaa tgaaaaaaat gatcaaagaa atggaatgac cattatccat ccaaataaaa
     2401 aaagaggatg ttccagaagt gatcaacttc aagaaataag aagaagctac ttctggaaca
     2461 ccgtgaatca gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaaacaa
     2521 aaatagcttc tcatattttt cgtttggtta ggcttattac cgcaaatgca agcttttgaa
     2581 caccgtttat tcgtaaaagg gagtgtgaca aaagtcttgt cacactccct ttttacgttt
     2641 ttattgttct aattgcgctg tcttgcttac tgcttcttgc ggtttcacaa atttctttga
     2701 gatcccccaa cctgtaaagg cactgaccat cgaaaacaca ggtgaaagca aactgaaaaa
     2761 cacgaatggc aagtaagtga gtgtaggaat gccaaatgta cttgctacga aacttccggc
     2821 gattccccaa ggcactaagt agttgattac tgttccacca tcttctagtg ttctccctaa
     2881 tacagttgga tcaaatcctg ctttttcgta agtttcttta aatgcattac caggcaggat
     2941 aacagataga aattgttctc caataaagat atttactccg atgcttgtca tcaatgttgt
     3001 aaaaatcaat tttggggtgt tcgtcatctt tcttgatact cgttccataa cggttgagat
     3061 caacccaaat tcgatcaaca aaccacctaa tgacaatgtt agaataatca atgacacagt
     3121 tggcatcatg ctttcgattc ctccacggct aagtagtgaa tcgatttgtt ggtttccagt
     3181 atttgaaaca aatcccgatt cgatgacccc tgccaacttc gttaaggaag tttgtgggtt
     3241 ttgaataaag atcattccta ctgctacagc aatattcaac aaaatcgtca aaattgccgg
     3301 tacctttttc catgcacaca agagcattaa aaatagagga atcagtgacc agatcgaaat
     3361 agtaaagttt gcttctaaaa tatcagtcac ttcacgaata gatgaaagac ttgcggcttt
     3421 acttgtgtgt cctaaaatca tgaataaaaa gaaagaaaca acaaatgccg gaacagttga
     3481 ccacatcata tgcttgatgt gtttgaacaa atcggcatca acaatagctg ctgctaaatt
     3541 tgttgattcg gataatggag acatcttgtc tccaaaaacg gcacctgaaa caatggctcc
     3601 aacaacaagg gctggattta gtcctagtgt tgtcccaata ccgaaaaatg caatcccaat
     3661 cgttgacata acagtaaagg cactccctac agcgctacct acgattgcac aaacaacaaa
     3721 tacagaaggg acgaaccact ggacactaat caatttaaat ccaatgacca tgagggttgg
     3781 aataattcct gcttgtatcc atacagcaat caatgaaccg actaaaataa aaataaagat
     3841 tggaatgatc cccggtttga tgccattgat gatcccttcg tgcagccaat ctgtcgggta
     3901 tttttttatc actgcgtaca acatgaccat tccgatagct gccaaaatcg tgatattagg
     3961 tgaaaccttt aatccgacaa cgccaatcgc aatgcctaaa acaatgacca tcaatacaag
     4021 tgttgcttct aaaaaacttg gtgttccttt tttttccatg ataatctctc cttttttagt
     4081 tgttcttatg ttctaaaaaa gttaagatat cggattactc cgccacaatt catccgcgat
     4141 tcctccttta taaaaataaa aagacctgct ttcaactaag gacgaatgac tccgcggtac
     4201 caccttaatt aaaaacagat ctgtttttca ctttcaaacg attattcaaa tgtatggact
     4261 ttccatgtaa ttcgtcaatt aggcttgatc ggttcacagc gaccaccgac tttctgaaca
     4321 aaacacctaa ccactactgc gtgaaaagtt gacttgtatg aagtttacct tccataagat
     4381 catttgtcaa tgaaaaagaa aatatttttt catttttctg aaaattattt ttataacttt
     4441 cgcgcacaac tttatctttt tactagaaag tagcgccctc ttgttttttt cttgctacta
     4501 tttcttgatg ttcttgtttg attttttcta atagcttggg ttctctcaat aaatctagag
     4561 ctgtattggc aagtaattcc gctgcaatcg caatagaagc aagtcctttt tcacttttag
     4621 cagcagcttt gaattcttct gtatgtccgg caatatatgt ctcagagatt gctacagtag
     4681 gctgtatcgt tggaataact tggctaacat ttcctacatc agaagaacct agacttgttc
     4741 tttgttccgt ttctatttct tcatcaggaa cccctgcttc ttttgcatgt tggaagaata
     4801 agtcatcgaa agaaggcgtt acgatgacat catcgactgc attttggaac aatccaaact
     4861 caaaagtcgt acccgtgctt aatgcagcac ctcgtacgat attttccact ttttcatata
     4921 ctttgtctaa tgtttgtcga tttcctgcac gtaaatagaa acgtccagca gcgtagtctg
     4981 gtatgacatt ggccgccacg cctccatctg taatgattcc atggatattg acatcttttg
     5041 gcaattgcaa gcgtagagcg ttgattccgt taaacacttg gatcaaggct tccaaagcat
     5101 ttactccttt ttcaggtgct gctgctgcat gagaagcgac accgtaaaat ttgatatcga
     5161 ctggatcatt tgctaaagat tctgtcgttt tcccataacg aaaagctgga tggacacata
     5221 atgctgcatc gacatcctca aaaaaacctt cccgaacgaa gcttccctta gctgatccat
     5281 tttcgcctcc ttcttccccc ggagtaccat acacacgaat ttctccgcca atctcatcaa
     5341 tgaattgttt cactatgctg gctgctaata cggagtaagt accaaataaa ttatgtccgc
     5401 atgcatgacc aatacctggt aaagcatcaa attctgccaa aaaggcaatt gtcggacctg
     5461 gtttattgga aatataacgt gcatcaaagc ctgttcggtg cccagctact tctttttcta
     5521 cccagaaccc ttctttttcc aattgtttga ttaaagctgc tgaggaataa aattcataat
     5581 tacttacttc aggatggtcg tggatagcta gtgcaagtgc ttgatattct ggtaaacggt
     5641 attgaatcag atccttcaat tgatttgttg agagtgtggt tgtcataaaa atttcctcct
     5701 atgctagtcg ttttttctta ttttctcccg aatgtttccc acgcaggtac gcttgaacct
     5761 tttgatgttt tttcgattac tttggctgtt tcttctgttt gataggcatc tactattttt
     5821 tgatacgttt tattgtcttc atcttctttt cttgcaacga tgatattgac ataaggacgt
     5881 gcatgctcat cgactggttc taagaagatt gcatcagaag cgggattaaa tccagcatct
     5941 acagctacac cgctattgat gactgaaata tctacatctg acaaagcacg tgctgtttgg
     6001 gaagcatcta actcagaaat gtctagattt tttttattct cggtaatatc agaaacggta
     6061 ggcgcttggc cttttgcctt gtccactttg atcaatccag cagtttgcaa taacagaaga
     6121 gctctgccgc cattcgtgac atcattaggg attgcaactt ttgctccatc tttgatatca
     6181 gccacatctt ttaacgtatt ggaataaatt cccaacggtg cattaaccgt attcccaatc
     6241 gatactaaat cagttccgtg ttcttcattg taattgtcta aaaagaattg atgttggaag
     6301 gaattcaaat cgatcgatcc atctgctaaa gccgcatttg gctgattgta atctgtaaat
     6361 ttcacatatt ccaaatggat gttttccttt ttcaaacggt ctttcacatc gtcccataca
     6421 tctgtatctt ccccaatgat ccctagtttt acggtcgttt cttttttttc agttgtagct
     6481 gcttttcctt ggctgcatcc tgccaaccaa ataattgcta caaatgtaat gactgcaaaa
     6541 cgaatatttt ttttcatttt ttctcctcca ttttcaatgt gtgacttttt taatcagttg
     6601 attactgata aattgactca aaaaaactaa gaccagaatc aataatgtag cgacaatcgt
     6661 tacatctgtt tggaatcgat tatatcctct cgatatagca agattcccta atcctccggc
     6721 acctactgct ccagccatgg tagtcagtcc gatcacattg atgatcgtca acgctgagac
     6781 ccgaatgata ccaggtaacc cttctaacaa ataaacacgt aaaatgatgc cgattgggct
     6841 cgtgcccatc gcttctgctg cttcgatcac cccaggatca acttctagca gtgcattctc
     6901 gatttgacgg gcaaaaaacg ggacaacgcc aaaaatcaaa gggactaaag ctgctttggc
     6961 accaatcgtc gttccaacta agaaacgagt agtcaaagct aaaagagaaa gcaagataat
     7021 gaaagggatg gaacgaatga tattgatcaa cttatcaagt atttcgaaca gcagcctgtt
     7081 agctagtaat ccatttggac gtgtcactac taacgtcaca cctaagatga ttccgatgat
     7141 cccaccaatg aatgccgtcc aaaaagacat atatagtgtt tgctttgtcg cttcgatgaa
     7201 ttcatccgga atcaaataga cattcggcaa atatgtttta aaaaattcag tcattttagt
     7261 ttcctccttc gatgactttg atacggttat ctaattcatt tttttgtaaa atagttactt
     7321 ttacaccaga tttaatcaaa taatccaatg ctttttgccg ctgtttggtt tctccagcta
     7381 aagtaacgat caaacttcct aagggtgttt cttgtaatag ctctacatta ccgtataaga
     7441 tattcgctgt tacttggtac ttgctgtaaa gatgagcgat tagaggttcg ttcgtctggt
     7501 tgccgatata acttaattcc accaaccatt tatttgcaat ctgatcggca aaagcagcat
     7561 gggcactgat tgtttccaat gcttggtcaa ggtgtgtcgc tgtccgaata aaatctttag
     7621 tcaattcttc ttcaggatga ctgaaaatct ggatgctgct acctttttca atgattttcc
     7681 ccgcttccat aaccgctacc ttgttgcaga tttctttgac aacttgcatc tcatgagtga
     7741 tcaggacgat tgttagacct agctgacgat ttagtttttt taacagtgca aggatctgaa
     7801 gggtcgtttt cggatcaagt gcactagttg cttcatcgca taacaacact ttaggttcat
     7861 ttgccagagc tcgtgcgatt gccactcgtt gtttttgccc ccccgacagt tgtttaggat
     7921 acgcagatgc tttttcttcc agaccgacaa gttccagtag ttcattcact ttttgacgac
     7981 gttcctgttt tgattttcca gaatacttta gagaaaaatc tacgttgtca aaaatcgttc
     8041 gactttccat tagattgaaa tgctgaaaga tcataccgat ggcctttctt tcttttcgca
     8101 attcttttgg agagagctgg cttaaattcg tctgattgac gatgacttct cctgctgttg
     8161 gtttctgcaa caaattcatc acgcggacta acgtactctt tcctgcacct gagtaaccga
     8221 cgatcccata aacatcccct ttttcaatcg ttaaatctac agaatctact gcctgtatct
     8281 ctttttcttt ttgtttgaaa catacagaaa tatctcgcaa ttcaatcatt aacattcctc
     8341 cttgtctata aaaaaagcat cctcaagaaa atgaaaatag tcattttctt gaggacgcct
     8401 ttttgcgtgt taccacctca tgttcaagct gtgttcacac acaactcctc ttcaagtacc
     8461 atcatactct agcaagatat cggtcgcagc cgtcaccgcc ttacatctat gctcgggatg
     8521 aattgctcga tggccatttt ccaatagtcg ttccttgctt cttctcagct accgaagctc
     8581 tctgtcaaag gttcttctat cgtacttacc atttcaatgc aaataatatt caaaaaaaaa
     8641 atccttttac taacataaac tatgtcagta aaaggacgac gtatcgtggt accacctttt
     8701 ttcggaagtt tccttccctc aagcccaaca tctcaaaaag agagattggc gttcgtttta
     8761 acggtcgaac atttccgaat ttgcctactt atcgacaaat tattctccaa ggccattttc
     8821 agcttttctt acttgactct tcacaccagc cagagcctct ctgcaaagtt tccaagctta
     8881 ctttccttct caacgaaatc aatattttgt tgttgggaat aatctacagg aggaaaactg
     8941 aaaagtcaag catgaaatcc aaattatttt ttctatattt atttttctgt ttttttatct
     9001 aacatttcaa taacagttcg aagatctttt ccttccgcaa gaaggttcaa tttttccttc
     9061 tcttcgacgc tataatttcg aacaaaactg gaatcgggaa tcaaaacaac gcctagtcct
     9121 gcacgtgaag cggctgtcgc tcctactaaa gaatcttcca ctgcaaaagc tgaaagagct
     9181 tctaatcctg ttcgttccaa agctgtcaga taagggactg gtgaaggctt gttctccgat
     9241 acttcatctc cgaacgtaaa cgtatcaaag taatcggcta attgaaagtg atccaagata
     9301 tctgttgctc ttttcgtaac agttgtcgta gcaatcccta gtttcatatg acgttctttt
     9361 aagtctgtca atatttcttt tgcatagggt ttcaattgga tagctccact tgtcagctgt
     9421 tgataaaaat attcttcacg ctttgctcgg acttcctgca ctttttcagg actgcctgct
     9481 gcctgcacta aaatatcaaa actttgtttc cagttttttc cgccccaagc gtcaacgatt
     9541 tctttcggga tatgaatatc aaattgctgg aatccccaaa gccaaccatc ccgatacgtt
     9601 ctttccgtat ccactaacaa accatctaaa tcaaatattg cacctttcat ctatacatta
     9661 atcctttcca aaacaataca cctagtttag catgactgat ccaaaatgaa cagaagcata
     9721 gagcccagcc gcatcactta taagagttct tggaatcgac ggatatagat tttcttcaca
     9781 gctgtcacaa gcgccagata agcgacgact gttataaaca accataacca gtaattgcca
     9841 gggagcggca tcaagcctaa ctcagcacca aatcctgtga atggaaggac tgaaccgaca
     9901 atgattccta atgtagtgat aaccagcatg gcaaatgttg cattgctttg gataaatggt
     9961 attttcggtg tacgcaatgc atggagtact aatgtttgtg tccacaggga ttcgacaaac
    10021 catcctgcat ggaataatgc gataaaaatc acttgttgtg agctatccaa tgtatggaaa
    10081 cttccaccaa ctacagcagg acaaataaca aaatacatca acaagtaagt agtgatatca
    10141 aaaatcgaac ttgttggacc aaaataaacc atgaattttc caatactaga tgcattccat
    10201 ttctttggct catccaaata ttctttatcc atccgatccc atggtaagga aatgcaagaa
    10261 acgtcataga tcagattcaa aaatagaagt tgcaaaggca acattggtaa aaatggtaag
    10321 aacgtactgg caatcagtac ggaaaacata ttgccaaaat tagaacttgc tgttgccttg
    10381 atatatttca tgatgtttcc aaaagtttct cttccagaaa gaaggcctct ttccaaaatc
    10441 gtcaaatctt tttctaaaag gatcacgtct gctgattctt ttgcgatatc cacagctgta
    10501 tccacggaaa taccgatatc agccactttc attgctggag catcattgat accatctcct
    10561 aaaaatccga cagtatgccc atttttacgt aaagctgtcg tcaatcgtgc tttttgctga
    10621 ggattcaatt ttacgaatac ttcgtgttct tcagctactt gcgctaattc atgatcattc
    10681 atttccgaga ttttctcacc agtaatcaat tcattgattt ctaggcctac ttgacggcac
    10741 acagaccgtg tgactaattc attgtcccca gtcagaactt ttacagctac accatgttct
    10801 tttaaagctt tcaacgcttg ttttgttgtt tcttttggtg gatcaaggaa agcaagataa
    10861 ccaatcagaa ccatgtctga ctcatctttg actgaaaatt ctccaacaac gcttggattg
    10921 gtcttttgtg cgacaccaat cactcgtaag ccgtcttcat taagtgattg tacttttgca
    10981 agcaccgctt cttttcgttc ttttgttaat ggtgcgacag taccaccaag atcaataaat
    11041 ttagaaatgt tcaacatttc ttcaatcgcg ccttttgtga tcatctgtgt tttgccagcc
    11101 ggatcagcga ctacaacact catacggcga cgttcaaaat caaaaggaat ttcatctact
    11161 ttttgataat tgattgtatc tgtatctaaa gtttctgctg cttcatcaat gatagccaca
    11221 tctaacaaat ttttcagccc tgtttgatag tagctgttta gataagcatg gcgcaaaaca
    11281 cgatcatctt catttccttc acaatccata tgatattcaa gaatgatttt gtcttgtgtc
    11341 aatgttcccg ttttatccgt acataacaca tcgatcgcac caaaattctg gatagcattt
    11401 aaatttttga tgactgttcc ttttttggcc atcacacttg ctcctttgac caaattcgtc
    11461 gtaacgatca ttggaagcat ttcaggcgtt aaaccaactg ccacagataa tccaaaaaga
    11521 aaagcttcta accaatctcc ttttgtaagc ccattgataa cgataactgc tggagccatc
    11581 aatgacataa atttgatcag cagcatggat gttttgtgga tgcctaattc aaaacttgat
    11641 tggatgggtt tttcagatag cgtttctgct acatgcccaa atagtgtgtc atttccgaca
    11701 gaaacaatga ttccttccgc gcttccactg atgacattgc ttcccataaa gacaagattt
    11761 tcataacttg tttcactttg ctgatcgtct tcacatactt tcgcatgctt ttctactgga
    11821 tagctttccc ctgtcaatgc tgcttgagaa atgaatagat cctttgcgga caataaacgc
    11881 atatccgctg gtatcatatc tcctgctgaa agccgaacaa gatctccaca gacgatttct
    11941 tccataggaa tctctgtata ctcgccattt cgtttgacag ctgccgttac tttcacaagt
    12001 gatttcagtt tttcagctgc ttgattagaa cgcacagact ggatcaacgt catcgtccca
    12061 ctgacgataa ccatagtgaa gataatggct acaccaaaga gatctttatc tcctggagcg
    12121 gctaatacgt attccgtaat gaatgaaata attcctaatg caatcaacac taatgtaaag
    12181 ggtgtgatgt atgctttcaa tatttctata atgaatggtg tttttttgcc atatgaaatc
    12241 gcattttcac cataagtttc tcttgaacgc tcgatttgtt cttctgacaa tccatttggt
    12301 gatgcatgga acaattcaaa taattcttgc ttcgtttttc cagcaaaagc tgcatagatt
    12361 ttttgtgtat ctttcttttt ttccatctcg atctctcctt tgtgtccaaa agacagctgt
    12421 tctcgctgtt tttggtcttt tgctattcat cggatcgata gagaaaatgc tgaaaggggc
    12481 tgagaacaga tagataatag ccctggcctt tcctctaaat ccttgttttt cgaatgactg
    12541 tcatccattt gttctcatct cctttactaa gtaattttaa ttcatgactt ccaatcattt
    12601 ttcgaatagt tattttccca actcgctcac ctcccttttt gttttttcac ctatgggatt
    12661 tgcggcaaca aaaaaacctc tcgccaaata ctcagcgaaa ggtttgaaaa agcaaatcaa
    12721 aaaaagcttc tcagatctca tcgttgagtt ttgacactgt acaacgtaaa aagcagatat
    12781 ctgcttttag ctaccttatg aaaagcctta tctcgacaat tcctgttcta cccattggcg
    12841 tctctcgacg tttttgggca gtagcctatg tttgtatagg agcctcacct aacaaatcct
    12901 attcggtttt ccctcgttaa tataacaact aaaaaagagg atgtcaaatc aattttccat
    12961 gtttttcata cttttttaaa atacacgcgt tatatttcct gtctcacgca gttttatttg
    13021 tctcattttc cacctcccgc tataatagaa ttatagtagg aaacgaggtg ctgttatgga
    13081 acgaacactt gttattatca agccagacgg cgtaagacgc catttggtcg gaagcatcat
    13141 ccaacgtttt gaagcaaagg gactggccat tgctgaaatg aaatttgcaa cgatgacacc
    13201 tgaactagca aaagaacatt atcagcactt gactgaacgt agtttttttg atgagcttat
    13261 cgattacatg acttctggtc cggtcgttta tcttgtgctg gtcggcgagg aagtgatcga
    13321 tatcgtccgt aagatggtcg gagcaacaaa aggggcggat gccgttccag gtacgatccg
    13381 aggagattat gcactgccgg ggactgaaaa catcatccat gcttctgatt cccgtgatgc
    13441 tgcaacaaaa gagatcgccc gctttttccc gccattcgat acaaacaaac cgtctgaaaa
    13501 agcggcgaag tgatacaaat cctaaactgt tggtctaact gctgggttta gcaatggcca
    13561 gcagtttttt ctctaaagaa ttcctttgat tcttccctaa tgtatattcg atcactgcga
    13621 aaaactcttg gtgattttcc gatttttctt cccctaaagc agcagaaatc agtcgttggt
    13681 aataatccgc catcaatgtt tcataaatca ctgtacttct ttctttatgc gctaaactcc
    13741 gtttcatatc ttcattcacg ctacgtgcct tttttaagcg accattttct atataaaaca
    13801 aagtcaagtt cgtcaaaaaa gtaaagagat actgctcgtg attattcagt tgcgcgatcc
    13861 gtcgcattct ttttttgatt ttcaaataat tattctcaat aaaacgatca ctgaatatcc
    13921 aaggaaaatc tgctagaata gatacttcaa attgcccata ctctggtact tgcatcaaat
    13981 attcttcaat gatacgacat tcataacgca aaaattcaaa ttccgtctca gatacttctg
    14041 tttttttgga catgtgcaga ttcaaaacca aatagtacag atcttctgtt ttcttatagt
    14101 gcctgcggtt attagcaatt tctttgtaag gatcaaagct gtcttcttga atccgatcta
    14161 gcattcgtct ttttttcttt aatttttttg gctcatcaag ctgatttcgt atccattcat
    14221 attcggcaag tgtcatgttc attcgctcta aaaatgattt caatgttcca aacgatactt
    14281 ttttcactga atttgtctcg atcccgacta gcgaagtttt agaacagatt ccttccgcta
    14341 attgggtctg tgtcagcttt ttgttgactc gaagcttctt aatcaattct ccatcataca
    14401 taatagttct ccttttttaa ttgaaattaa cccttggctt ttccagaatg cttgccaata
    14461 agaggaatga ttatttcaat ttaaattttc cgcattcaat aaatagatac gttaaaaata
    14521 aaaaatacac gaaaaaaata aaaaaatgtt tttctcataa aaaataccaa ccttcaagca
    14581 tgagactgct gtctaatccc tgaaggttgg tttgattgtt tattgatcat ttttttactg
    14641 ctccgcattg gttcctgtta ctggatatag gatagtcgtt gttttattgc cgttttttga
    14701 aaagacactg gtcgaatgtt cacctttttg cttttcgatt gcttcattac gttccgcttc
    14761 gtttttatag tcatattttt tcggatcaat cgtctcaaat ccgtttggca cataaaaccg
    14821 caagagattt tcctgattca gcttatctga aagacttaac gcagtcttga ctttttcctg
    14881 atctttggag atttcctcgt cgattcctgc tgctttcgta tcgatgattt ctcctgtctg
    14941 attattgtaa gcttttcctc ctaggacggt gtatttgggc gtcacgaagt tcccatttcg
    15001 aaaagcaact acttgatcgt gctgagaaga taataagtct gtaccaaaat gaagataatc
    15061 tttatcatcg attcccaata gatgtagtaa tgtcggcaaa acatcgattt ctccaccata
    15121 ttcatgattg actgtccctt tagtgtaacc aggcatatgg atcatcaatg gcactcgttg
    15181 catctgtgcg ttgtcaaatt catcccatgt atctggatct ttaccaagag cacttgctaa
    15241 atctttatta tctgtattcg agatcccaaa atgatccccg taaatcacaa agattgaatt
    15301 ttggtaaata ccagattttt tcaaatacgt aaaaaattgt tccaatgatt gatccagata
    15361 atgggccgtt cgaacataat tatctactgt gctattgcca gtattcaaag atggaaagtc
    15421 gaaattcttg tcatccgtat aataaggcgt gtggttggtt acggaaagaa atttcgtgta
    15481 aaacggctgt tgtagatgtt caagatattg ggcagattct ctgaataaat ctttatctaa
    15541 tatcccataa cctaacgttt catcggattc atcaaaataa gatcgatcaa agaaattgtc
    15601 ataccctaaa tttttatata catgatctct attccagaaa ctagctacat tcccatggaa
    15661 aacagcactt gtatagcctt ctgtttgacc aagggtcgct ggtgctgctt gaaagacatt
    15721 atctgaccca agttctgtga ataatgagcc ttgaggtaaa ccaaatgtgc cagtttccaa
    15781 catgttttca gcatcacttg tctttccctg tcctacttca tgaaagaaat tgtcaaaact
    15841 gatcgttgct tgattctgga aaatactatt taaaaacggc gtgacttctt gtccgtctac
    15901 cttcatattg atcaaaaact gttgaaaact ttctaaatga aggacaatca cattttttcc
    15961 tttagcaatc ccaaatgttt caggatttgg ctctgcataa tgtttctttg tatagtctaa
    16021 aacttcatca attccattac tgcttgcatg cgcgcgaaca ctgctagtca ttcctgtttt
    16081 gattccgtca taaatagtgt acgcatcgat tcctagatat ttgacaatat aggaacggtc
    16141 aaatgtccgc tgtagcaatt ggggacggtt ggcttcgctc aacgctaaat ttcctgaaaa
    16201 aatcaatcct gaaagacagg taacagcgat agccatcggt ttgctgacag ggttgctttt
    16261 gataggtatt ttcttgacct tcaaccaaac cagtagccca atgaacacag cgatatccaa
    16321 ccaatagatg acatcatggg gtttcatcaa agaaaaggag cttcctgaca acccttgtga
    16381 cactttcgaa aagcctaata cagatttgac tgtaatgaaa tctgtgaatt cccgataaaa
    16441 aataatattc aagtacaaca aaagcgtatt taaaatatca atgatcaaaa gaatggctaa
    16501 cgctggcttt ggctttttaa tatacaaaga aaggccaaaa aacaataagg tagtcgcaat
    16561 cgggttgatc cacaagataa agtactggat cgtaccttcc acacctaatg aaaaatctgc
    16621 atagtaggca atgatcgtct tcagccagaa aaagaaaaca gctgtcaaaa cgattgctaa
    16681 acgattttgg cacaattttt taagttgtgt cacgtatatc acttctctct attcacattt
    16741 tttcttattt tgctcctgaa aaatgaaaat ttcctaaaaa agttgttatc ttaagatttt
    16801 tttattcttt ctaaagatta ttccattcat ttttcacagc ataaaatcgt ttcggtgtaa
    16861 gcggttaaaa tagtaaggag ataaaaatct tgtggtgacc gatttactct atagtattca
    16921 aaagtagttt ctcggtattc gtacaaaaaa aacatactaa gattagaggg gaacatcatt
    16981 gacgaatgat gttctcctct attttctatt tttagattat agtgaagagt gaaagaaagt
    17041 tcgaacaaag ccttgaggac tacgagtccc tatcaaaaac cgaacttctt tactgtcatt
    17101 ttagaatcag gtttacgtat gttggggcca tgagcccgtg tataggaaat tggaatcgaa
    17161 atgaagcagt aaagttcttt caacacttaa aaaggaggaa aacaaatgcc ttacattctt
    17221 gcattagatg tttcaatggg acacagctac agtgtcctat acaaagacga cacctgctta
    17281 tttgaagacg agatcgaaca cactcaaaga ggatttcatg cattactcga agagatccag
    17341 cacttgcctg aacgtccatt gattgttttt gaagcaacgg gtatttattc cagaccagtc
    17401 gaaaagttct ttcaagatca tcacttttct tactgtttgt taaacccgtt agaagcgaaa
    17461 aaacagatgg aagaaaacac cttacgcagt tggaaaacag ataaatcgga tgcccatcgg
    17521 ctcgcacaga ctcatcttaa aaatcaacgt caaccaaaag aagtacaaag caatttttac
    17581 ttagagatgc gcgatttagc ccgtttttat caagaaattg agaagaaaat cactcgactg
    17641 cgcatggatt tacacaactg ccttcagttg acttttccag agcttgaaca gttcttttca
    17701 aacaggctga ccccttacgc attgacattg atccgtcttt ttccccatcc agattttgta
    17761 ttggcatcta ctcggacaaa aataaaaaat aaactgatca acgaaacacg ggaaaaaatc
    17821 tccgcgaatc gtgccgagca aaaggcagat caaatcatcc actatgcaca gtgtgcctat
    17881 ccagctgttg aaaaagacag tattcactgt caaaaaacga tctattacgc cgaacttctc
    17941 caagatttac tcgaacaaaa aaaagcactg gcgacacaaa tgatcaaaaa agccgaagcc
    18001 tctccttgct ttttgttgta ccaaacgttc cctgggattg gcgcactcac agccgcttta
    18061 ttgctcggtg aattaggaga tatcacacga tttaaaaccc ataaacaatt gaatgccttt
    18121 gtcggaatcg acatccgacg gtatcattca ggtaagtata ctggccaaga ccgcatcaat
    18181 aagcgtggta atcccaaagc acggaaaatt attttcttca ccattcgaaa tatgattcgc
    18241 cagcaacgcg cagctcctaa tcatattgtc gattattact acaaattaaa aaagcaacct
    18301 atccctaaga aagataaggt tgccacagtt gcttgtatga acaagcttct gaaatgtatg
    18361 cacgctatgg ttagggcaca tacagagtac gactatgcgt acgcggtctc agtggaccat
    18421 taactaaacc acttacagta taccacttct ttgcccagat cgaaaatttt ttacgaacgg
    18481 ggctttattt agtgtgcctt gattttgtcg ctttcgttta cgagctattt ttcttgcacc
    18541 ttttacttga ctaatcgtag gaaagaggct gtgacaaaat tcttgtcgca gctccctatt
    18601 tcttgatgca ggacagcttt ttcacaacct ctccactaaa cggtgttcaa tcagctgacc
    18661 ttcggtatta gctcaaaaaa cggcaaaata tgaggagcta ttttcccgtt tttttctcta
    18721 atactcaggt caaaccgctg atttcacaac ctctgattca cggtgttcca gaagtagctt
    18781 cttcggaaat aagccgaaat tttacaaaaa cttgagaagc acttttcgta aatttcttct
    18841 tatttcttga agctgaccac ttctgtcaca acttctgttt ttcattgtcc ataggtttga
    18901 tattgtcctt ctgatgactc attcgtaatg attgaggctt gattttcttc atttcccgtt
    18961 atcgtttgtc cactgccaga atctgaaggg cttagttctg tcgactcgtt tttttgagaa
    19021 gaagcagttg ttttcttgac ttgttcgttg gtctttttcc attccgctag tcgatcggcc
    19081 accacttttt tgtccacttt ttcagaataa gctgtaacca cttttccatt tttcaaacgt
    19141 gctaccttgt cgccatcatg agtcaaaagc aaaacgacca aatgtttgtc tttgaattga
    19201 tattgtctga gagaaatcgc actaagtacg tcatcgatat tgataaattg attttcttct
    19261 tttccttttg gcaaatagat gtccaattca cctttttcgt aattggcagt taatttatat
    19321 tcctcccacc aataagttga gaattctttt ttgaaattat ctactgattc tttgcttgct
    19381 acgatttttt ctgtttcttt ctgagcaaga tctgctgtca tttttcctga aaatgctgcg
    19441 gataaaataa gcagtaagaa aaaaccgaat aaccaaaaga gaagaaacgg tctttttttc
    19501 ctcatgcctt cctccactcc tatttaattt tgactattct attatataca aaaaatgaaa
    19561 tagaaagaca aaaaagttga aaaagatcgt aaataattga acttcttttt cctgaagcgg
    19621 taagaacggg aattttcata cattctctct tggctattct ttaagcttca aatccatttc
    19681 tgtctgatac atctttgaac caatgacccg atttcttgat cgttcttttt ccatcttgtg
    19741 ctaaatctac tgaaataaaa ccgtaacgat tcttataggc attggtccac gaccagttat
    19801 ccatgcatgt ccacatatga taacctaaac aattggcacc ttcttgaatg gcttgatgga
    19861 catatttcag atgagattga acaaattcga tccgataatc atcgtggatc ataccatctt
    19921 ccttgataaa tcgtgcttca ttttctacac ccatgccgtt ttcagaaata aaacagtcga
    19981 tattcccata atgatctcgt acattgatca aaatatcata gattcctttt tcatagatct
    20041 cccatccgcg ataaggattc attttacgac caggcatttc ataattgtcg aagtaatcct
    20101 caggcatagg accgttttta gtatcgattg gtgtttcttt tgcttttatt cggcgtggtt
    20161 ggtaataatt aattcccaaa agatctactg tgttttcttt aatgatcgtc aaatcatctg
    20221 gatgaatctc cggcattaga tcgagttctt ttattctctc aatcaattca ctgggaaact
    20281 cgccttttac agctggatca aggaatgaac gattgaagaa cgcatctgct attcgtgctg
    20341 ctttaacatc ttcgggattt gattcgtctc ttggataact cggtgtcaaa tttaagatga
    20401 ttccgatcac accgctttga tccatttcat ggtaagcttt gatggctaat gctgaagcca
    20461 acgcttcatg ataaccaact tggattgcct ttttcatatc tgattcttcc ggataatgcc
    20521 atcgatacaa atagcctgcc tcgactggaa cgattggttc attatgggta aaccattttt
    20581 ttactttcga gccaaacaat tgaaaacaac tttttgcgta tttttcatag gcttcaaccg
    20641 tttctcggtt caaccatccg cctttttctt gcagatacat cggcatatcg aaatgataca
    20701 gattaacaaa gggttcgata ccgttttcaa tcaactcatc aaaatagtga tgatagaaac
    20761 gaacggcttc ttcgttgatt gttccagatt catcaggtat caaacggctc cattggatag
    20821 aggttcgaaa tgaattatgc ccagtctgtt tcatcaactg tacatcctct tgatacttcg
    20881 tataaacttg cgaggttttt tcaggtccta cttgatcaaa gaacttttgc ggttcgtttt
    20941 tgtaccaata atcaaaaata ttctctcctt ttccatcgcc tgaaattctt ccttctgttt
    21001 gtggaccact tgctgcagag ccccaccaaa aattttttgg aaattgatat cttttcatca
    21061 aagcacctct ttttcttttt tagttgattc catgtatgat attttccatc acaatcactt
    21121 tttcattttc ttcctcttct tttttgattc gtttcaacag ctgttctgct gctcgttccc
    21181 cgattttatc tgttggtggc gtgatcgtga tttttggaag gatactcaga tcgtttttat
    21241 ctaaataatc atagtgactg aacgcaatat ccttcccgat tctgacacct tcttgattga
    21301 aacattcgat ggcaccaatc gacatgttgt aattgctgac aaaaatagca gttgcccctt
    21361 tttctaataa tgcttttgcc ccgtaatagc cgctttttct agaatagtct cctgtaaaga
    21421 tctctaaatg gatagacgga tccttttgta ccgcttcttt ggctccagct aaacgttctc
    21481 ttgctacgta atctgtttga gcggcagcaa taaatcctat tgttttatgt ccttgtccgc
    21541 catgatgagc aatcactttt gctacacttt ctcgatcatt cactactatt gtatcaatac
    21601 ctgctggccc acccggacaa ttcaatgcga caactgggat actcattttt gtcagcattt
    21661 caaatccaga ccactcttct tctgataaga agacgatcaa tccatcgatc gcatgcttca
    21721 tcaggtattc gattttttgt tcgatttttt ctggctgatc ggcaaacccg cttaataata
    21781 agctataccc attttcttca gctactcttt cgatccctga aacaacctcc gaaccaaatc
    21841 ggttagatat cccgtttatc aacagtccaa tagagaaact tcgattattc ttcaatcctt
    21901 tagcaatgat gttttcttta tagtccaacg ctgaaatcgc ttctgatatt ttttccatat
    21961 tcttttcctt cagctgctga ccgtttaaat agcgggaaac tgttcccact gaaacaccag
    22021 ccatcttagc cacgtcacgg atcgatgcca tacattttca ctcctgccag ttacatttga
    22081 tttctttttc ttgttgttcg accatgatga ccgctttctt tgcataatgc cgcttgtcat
    22141 caatatccat ataactaaag tcttcagaag tgagttcaaa aacaagcagt ctttcctctt
    22201 gagaccgcaa ttcatcttta cgaaaagctt tcaataattt cgttctttgg atgacaacgc
    22261 cgtcaattaa tcggacatag acaagagtac ttgttttacc tgaaacactt cctgtatttt
    22321 taactttaat tgatattatt actttgtcaa tatccgtttt gacttccatc tgactgtatt
    22381 caaatgttgt atagcttaga ccatgtccaa aaggataaaa tggacttccg cttaaatcat
    22441 aataattctc ttgtttagaa gcagcacgct gatagtaata aacaggtagt tgccgactat
    22501 ttctaggata agacaaactc aatcgtccgc ttgggttatt tcttccagaa atcatctgcg
    22561 caatggcttt tccaccctcc tgtccaggaa accatgctgt tgcaatggca ttcgtaaatg
    22621 actcgatttt catcaaatcg tagggtcttc cttgcacaag tatcgtgatg attggcttgc
    22681 ctagttgata caattgttca atcagctgtt cttggtatcc acctaaagaa agagaagcaa
    22741 catctacatt ttctccagaa tccatgttga tccctttcga tgaaactgcc ccgttacgaa
    22801 gaaattccat atcaaagttc cgcgcacttg atccacctag aactgcaatg atcacatctg
    22861 agttttgagc aagggtcacc gcttctaata atttttcttc ctgatcctcc tgatttctta
    22921 cttcacatcc ttgtgcataa gaaacctttg attggggaaa ctctgttttt atctcttgta
    22981 ctatcttttt tttcaaacgg tcttctgttt gtggtgcaga gtagtctccc aacaagtggt
    23041 aaacatcgtg agcatttgga cctataacag caatgttttt tacttgttga tccaacggca
    23101 atataccgtc attttttagt aaagtgatac tttcttttgc tgtctctaaa tttaagtgct
    23161 ctgattcctc catgagatga tcccaatact cctttggatc gtttgcaaat ggctgttcaa
    23221 ataaacctaa taaaaatttg acagataaaa ctcggcgaac tgcttgatct agtagctttt
    23281 catctacgac ttgattatca atggcttctg caatcttggt ataggtctca tcccacaaac
    23341 tgagatcgat tcctgccgct aatgcatatg ctgcagcagt ctttttatct gtaaaaacat
    23401 cggataaacg atccaaagcc acaccgtctg ccataacgat tccttgataa ccgatctctt
    23461 ttcttaatgt ttgctcaaaa agcgctcgat tgatatgaca aggtatcccg tcgatgtcat
    23521 tataagcagc catgacacct actgcatttc ttgtactctt caataatggt ccgtaaatat
    23581 cggagaactc tctgcgtcca atcgttactg tgcctgaatt atgaccgcct aaagcttctc
    23641 cttgtgcgat acagtgcttt aacacaacac cgatctgatc cttcttctta ttgatttcct
    23701 ctactgtctg atcaagaaaa gaaacttttt ctgaaataat ctctccttgg aaccctcgaa
    23761 cgaccgcttc agaaaaacga gctgagagaa tcggatcttc cccaaagcac tcttctgtcc
    23821 ttccccaacg aggatctttt gccagatcca aagtcgacac cagcgctaaa tgaacacctt
    23881 tagcagccag ttcttttgcc atcaatcgac tcgattgttc taataatgct acattgaaag
    23941 tatttccacg gccgatattc gttggataag aaatcccatc caagccttga tgaccatgtg
    24001 gacattcttc tacaagcaaa actggaattt tccatcgcga atgctgtagt acatattctt
    24061 ggactaggtt agccaccttc cagctttctt ttgcaggaat cccattctta taatctgttt
    24121 tagaccacgg atcagagcga aacagaccgt ataaggcacc cagaccgcct ccccatttaa
    24181 catgttcttt taacttttct gttaattcga tcttgtctcc gtttttttca taacattccc
    24241 aaccatacaa atgctggttt acctgtccta ctttttcagc aatattcatc ctcgaaagta
    24301 aatcttctac tctttggcgg atggttaact ttgggttttg atacgggtaa tcgtacacct
    24361 taatatcctc cttctgccaa ttttgcctta ttagcggcta atacaaatgg caggaataaa
    24421 aatacatcta gaatgataag tattactgtt aagactgctg ctcgtatatc gcctcctgta
    24481 gcaataaaag attgtataac cggtggagtg atccaaggtg cagcagcaac tgttttactg
    24541 atgattccaa gacttgtggc tgcgtaggca ataaccaagt taatagatgg tataatagcg
    24601 catggaatca tatatattgg attcatgact actggtaatc caaacataat tggttccgta
    24661 atattaaata aacaagtagt cagaccaaat ttagcgattg atcgctgttc ttgacgttta
    24721 gaagcccaca agatagcaat aactaacggt aaaatacacc cgccacctcc gatcaatcca
    24781 aatgcattgg taaagggctc cgtaataata ttaggaattt cttttcctac tgaaaaagca
    24841 tccatgtttt cctgaataga ctgtaacaaa ggtgcttgcc gtataggagc caaaatactc
    24901 gaaccatgca taccaaacac ccataagaaa tcagagaaaa actgtaaaaa taagatgcct
    24961 gggacggata aaacaaaagc tttcaatggt gtctgaatcg ctaattcaat taattgcgga
    25021 acattcaaac cagtcgtatg gtttaaagta aaaacgacaa ctgaaaaaaa taaaatgaca
    25081 actatttctg gtactaaaga attaaatgac gcagctactg ccggtggaac tccttctggc
    25141 atcctgattt taaaactctt cattttcgct aatttgataa ataattctgt actgacaagg
    25201 gcagcaatca tcgccgttgc taagccagac gaagaagtca gactttgtaa gaaaacccct
    25261 gtagcttctg ctgtatttcc gtccatcaaa gtgactgtac ttgataaaga catcaaaata
    25321 aacattacgg ctgtacttag aattcctgca tgaacaggac taaatgatgg atcatcaatc
    25381 acgcctgttt ggatatagtg ggttgccaaa ttccagccga tattgtaaca cacgataata
    25441 gctaaaatac tcatcgtacc attattcacg attgaagcaa gttcagccaa tggacttaga
    25501 tcaagtattc tttggactgt tgcatttgaa aaaatcagag cattcagcaa aaccatgaca
    25561 gaagcagcca ttactaacgg catagtaacg ataaaactgt ctcgaatggc cagcaagtgt
    25621 cgctggctac tcatcttaga tacgattggt agcatttttt cagatagtct gtctacgact
    25681 ttcattattt catctcctag attctaaaat tgaaccggtt catttttaat atagcacttt
    25741 ttttaaagat tgcaacacat ttttttaaaa cgctttctaa aggtggtgga ttttttatga
    25801 cttttgaaaa attattctat ttaatggtat tttgtgaatt ttttctcttc catccgtttg
    25861 aagttttact ttccaatggt ataatttctt tatcctatca aatgggggaa gtattttgaa
    25921 gaaattaatc gtactattta ttttgtctgc gggacttttg acattgactg gctgtgattt
    25981 gtttggcggc tctgaccaat cctcggacga aacaaccgat tcttctgtct taaaagcgga
    26041 aaaagcagat atcaccaaca gtgtatatac gctaaaagta cgtaaagata tggatttatc
    26101 cgcgggacag ttgacattta cagataaaaa aatggaatgg acacgcacgt atcagacaca
    26161 aggagctgat tcttctgaat catctgaaga aaaaacagtg ctgacagata tcaagattac
    26221 taccaaagat gacgaatata ttatttcagg aaaagaaaat aaaaaagaag caacagtcac
    26281 atttaccaaa atcggtaatt accgtttgaa ggataaggat ggcaacatct acagtctata
    26341 gtgccatctg ttggatcttc ttttgtttct ccccttcttt tctgctatag taaagaagca
    26401 agatgactgt aaaggagacg aaatggatga tcgatagtcc tgattattgt acatgtaata
    26461 acaacaaaaa tatccataca gaatttaatg atcaaggaag tttgagtata tgcgacaatt
    26521 gccacaaact agtcaaagat tcttttaaat cgtttgaatc ttatcctgtc aaaaattatg
    26581 taaaaagcaa aagaagataa gcaaaatcaa gcaaaactca ctatggctga tttttatgaa
    26641 cttcaaactc agcttcttcc tttgatcaag cttcaaaaac tggaatcagt aaaacaaaca
    26701 gtggatcatt tcagttcaca aatcaaagag taacgtttta tttatttcac atctttgaat
    26761 ataaaataac caaacgatag aaagacttat tctacatcac gtagtaattt ttctttcccg
    26821 tcgtttggtt ttttactttt agcctaattc ttgacctttc gcatgctgat tatctgtaaa
    26881 atattttcca gcaggctttc cttccaattc agctaatttt tcttggatct gacgaatcac
    26941 cttttttgca tcttctggat tttctacaaa atcgaatgta tccccatcca cttgtatttt
    27001 aggacataca tcaaaatcct cataccattg attatagcgg gtgttcagtg tttcgtaata
    27061 ggtgtataac gtttcatcgc tttctagctg ttcatagtca cgcccgcgtt ttttgatacg
    27121 ttctagcatc gtgtccaaag aaactttgat atggaccatc aaatcaggac gtttttttgg
    27181 agcaacatca tctaactcgt ttagcatcgt atctaataag ctatcgtact gctgtacttc
    27241 gatatccgtt acccgaccta aatcagcatt tagatggaac aacagactat cttcgtagat
    27301 ggagcggtct agcacattgt catcgttcac taaagcattt ttcattgcta aaaatcgctt
    27361 attcaaaaag aagatttgca ataaaaatgc gtactgctca ggatttgaat aaaaaagcgg
    27421 caatacttcg ttatcttcga cattttcata aaatggttta gaatcaatct cttttgcaag
    27481 catatccgtc aaactgctct tacctgcacc aatcgttcct gaaatcaaaa tcactgacat
    27541 acaacccttc ctcttctata agtactcgcc tacgtaagtt tcaacaggca atttttcaaa
    27601 atcatattca cgtacagctt ggtctgattt taaatacgtg tgaagacttt gtgtaatcat
    27661 caagttcgta ggtaccgtct cttcttgtac gatgatcatc ggttttttca acatagttgc
    27721 cacacctaat tcgtaagccg ttcctggatc gattgtttga tgctcaaagt caaggatagc
    27781 caaaacaaat tctgcatttg tcaactcttc catatctttt tcgtatactt cctgcgccca
    27841 tccagcgctg aatagttcat aattgctttc ttggtgatga cgtggagagt agaaacttgt
    27901 gactgttttg ttttcctcca acgctttttc gatacgactt actcgatcga tttgctcctc
    27961 tgaaaaaaat ggtcctgcta aatatatttg actcattatt ttgcctcctt caataataac
    28021 tgatttttaa tagatactgc tcgtaattca ttgaccagtt ccaatggatt gttggaaaag
    28081 atcacttgat ccaaccattt tttatccata aaatcctctt ttgcaaaagt ttctaataat
    28141 tgttgcaagc cgctgtaata accgccaata ttcaatattc cgattggctt atcaggtgtc
    28201 aggccgatcg ctatccaaga aagaagttgc gaaaattcct ccaatgtacc tagacctcca
    28261 gggaaaacga ggacagcatc tcctttttca aataataata cctttctttc atcaattgtc
    28321 tctgtaggaa taaatgtagt aacatctttc ccaggcagct cagtttcaaa aaggccattt
    28381 ggatagatac ctgtgacttt tccgttattt tccagtactg ttttactaat cactcccata
    28441 atcccggagt atgaaccacc gtaaaccagt tcgatttctt cttgtgccat atattttcct
    28501 aacgtttgcg caataaattt gtaactttct ttattgccaa aacgggaacc gcaaaaaaca
    28561 gtaagtttca taaaaaaaca ctccctaact caaaagtcta tcttcattct acacgtgttt
    28621 ttttaaaata aaagactaga ttttgtagtt ttttagtggt atatagatca aacaccacta
    28681 tatatagtat ttgcaaaaaa ataatttttc agctatttta cagtaaccaa aaaaataaat
    28741 aactagtaaa tataaaattg aaacgactta cttatagaag aaattgggat ttcgtcctgc
    28801 atgtcaataa cacgcattat ttaaatagtc acacacttgt ttctcctatt aatgaaaaat
    28861 ccattttatt ctcgtgttat gtttcaccaa cgcctgatgt ttctctaaat aaaaaagccg
    28921 acgaaccaaa taatcgaaat ttgatcattt tggttcatca gcttgttccc aaacctttgt
    28981 attaacgata aatagctgga ttcgtaaaac tatcacttgg atcatcactg ctggaagaaa
    29041 attcggtgat aaccgcgcct gttgcacctg cctggaacca gtgtttaata ttttgttcga
    29101 tcgtgtactg atctccagcg tgaagtatga tttctttttt tgccgtatag taacgttctt
    29161 gcccctttgg aataatacag gaaagttttt ttgtttcttt tccttcgaca taaagatata
    29221 ctaagccttt tcgacaacga aaggtttcct tttttccttc attctcatct cgaggtgggt
    29281 gccgatgctc tggacaagtt tgataaggca ataataccag ttctttccca cagtatcgct
    29341 cattattttc ataaacaaat aaattcaatc cttcagtctc gattgctcct aatccaaaat
    29401 ctgtatattc gattctttct aattctgttt ctttcaatac tactcctgaa tctaggaaca
    29461 tttgttgtac tcttttcttg taatccattc tgttcctcct aactgattgt caagtcatcc
    29521 caagatatct cttcttcctc tttttcttct tggatgactt ctgcatttgg gtcaactggt
    29581 tttttcaata ataaaatagt tattcccgta gcaaaagaac cagctaacaa tgcaccaatg
    29641 aatgcccatg gtctggtcat cgtagcgact gcaaacattc ccgatgccgg aatacggcta
    29701 tctgcgcccc aaaacatgct caatccaccg ccgactgccc caccgactcc cgttgctatg
    29761 acattgcgtg ccaagtcgtt caatacgata gggaaacatc cctctgtgat ctgacagatc
    29821 cccataggaa aagcggtctt caatgtatcg atttcttgac gattgaatat atttttacga
    29881 atgattttcc ctaagaaata agcgatcgtc aagccgaatg gggttaccat cgaagctaaa
    29941 atcaatacag tgattggttc atagattcct tctgaaaatc gtgccacacc cccttatcca
    30001 gaataaatgg taatccagaa acagccgggt atctgcggta ataagcctaa ccaaacgaaa
    30061 aatatgagaa gctaatatga gaagctattt ttgtttggct gttcttatta cttgatgcag
    30121 ggcagctttt tcacaacctc tttttttatt tatgtttcat aatcaatatg gtaaggatgc
    30181 agctccgtat ggtagaccgc ctccgaatat tcaatcattt ccccaatttg cgtagacgat
    30241 ttccgtatcc gtttgatgcc gatctgtgcg tctgttttca gaatttcttg ttcttcttct
    30301 gttaaaaata ctgccgagaa atcatcagaa aatgagtcaa tagtatatcc ttttttcgtc
    30361 agcaaacgat acaaagattc tttgccaaat tcttctctgc tgaattgcat catttcttgt
    30421 ggaagataat gaacaaaata aatataaggc tgattgttca aataatataa gcgcttaaaa
    30481 caaacagctt gctcaccgaa aaaggaaaac aaaggatctt ccttctttat atggaccaat
    30541 tgcaaatcca gcgtttcttt ccggacttca tatcgggagt tctccaagat ttgcgtaaag
    30601 cttgccgctt tggataatcg attgtaagga cgattgctta gaaccgtcgt cccacgacca
    30661 cttttctttg cgacatattc atctgatgcc agcagttcga ttgcctttcg gacagtgatt
    30721 ttgctgactt gaaacagttc ttctaattca ctttctgtcg gtaacatcga accgactgga
    30781 tattttcctg agaaaatatc ttctttaatt ttatctgcga cttccaagta tagtacacgt
    30841 gtccttttca taacgtcacc ctttgcttta ctattactaa aagaatagca cataaaaaat
    30901 aaaaaaagaa cacaaatcac atgtgagaag tgtgttcttt ttcattcagc tgctttaatt
    30961 acaagtttac gccatttgag gcaattactt ctttatacca atcaaaagat tttttctttg
    31021 atcgattcag tgtcccattt ccttcatcgt ctagatccac atagataaag ccgtaacgtt
    31081 tactcatttc tcctgttgat gcactgacaa gatccataca tccccaaggc gtatagccca
    31141 tcaaatctac accgtctgct actgcattag ccatcgcttc gatatgacga cgtaaataat
    31201 cgatccgata atcatcaata atcgtgccat cctcttccat atgatcaatg gctccaagac
    31261 cgttttcaac aatgaataat ggtttctggt aacgattgta taattcattc aatgcgattc
    31321 tcagcccttc tggatcgatc tgccagcccc attcacttgc ctcaagaaac ggatttttta
    31381 ctcctcccag caagttccct tctgaagttg ctgcttgtgg atcagtctca ttgattgccg
    31441 tagacatata ataactaaat ccgatgtagt ccactggata ttcttttagc aactggaagt
    31501 ctttttccgt ttgctctaat ttttccggat cgactccata ttttttatgg gtacgcgctg
    31561 tgtatgcagg gtattctcca cgtacttgga catccgtgca gaaaaaattg aattcttggt
    31621 tttggatcat tgttgctgcc tgattgactg gattggcatc gatgctgtaa gttgttgcat
    31681 agataatcat gcagccgatt tggatatccg aacgcaattc gtggccgatt tttacggctt
    31741 tgctacttgc aacgaattga ttatgccatg cttggaaaat attttggtac tctcctgctc
    31801 cattgctttt tactaatcct tgacttaaag ctggaaagtg aaaggccgaa ttgatttcat
    31861 tgaaagtcat ccaatatttg actttactgc tgtatctctc taatactgtc tgagcaaatc
    31921 gttcatagaa gtcgatcagt tttcgatttt tccagccacc gtattctttt gccaaatgaa
    31981 gaggcatttc gtaatgagaa atcgtgatca caggttcaat atcatatttt aagcactcat
    32041 caatcagctg atcataaaat tctaatcccg cttcgttagg agtaccttcg tctccatttg
    32101 gaaaaattcg tgtccaggca atcgaaaaac gataacattt gaatcccatt tccgcaaaca
    32161 aggcaagatc ttctttgaaa cgatcgtaat gatcaattcc tcgatgattc gggtaaatat
    32221 atttttcttg atcaatcgtc cagtcaaatt cttcactgcc gataatcgca aagcgctgct
    32281 tacctccagg catagcatct gcaacggata gcccttttcc gtctcgatca taagcccctt
    32341 ctaattgatt ggcagcagtt gctccacccc ataaaaattt ttctgaaaaa cgtgattgca
    32401 tcctgatttc ctccttattt tacaattgcc agaacatctt cgtttgcaat aatctctttt
    32461 tggtctaatt caagtatatc ggcaaatgca tcagtattcg taatgacaac tggagtgatt
    32521 acgctatagc cagctgctct aattgcttcg atgtcaaaac gaatcaataa atctcctgct
    32581 ttgacgagat ccccttgttt gactaagatc tcaaagccat tcccatttaa ttctactgta
    32641 tccatcccga tatgaatcaa aatctccgct ccgtcagtcg ttgtgatccc tacagcatgt
    32701 ccagttggaa aaatagtcgt gatctctccg tctgcaggtg caactagttc tcctacttct
    32761 ggatcaatag cgataccttt tcccatcacg ccagaagaaa aaacttggtc tggtacttca
    32821 ctcaatggca gtactttacc cgttaaagga ctttttaagg tatgccgtgc tgaagtaatc
    32881 ggttctcctg cattcgcatg tttgttttct aattgattgt cttgtgtttg ttcgtcaaat
    32941 cctaaaatca atgtacctac gaatgcaaga acaaaagcaa ttcctgtagc tattactgct
    33001 actgtcacat ttgactctac accatccacc gtattaatga aagtcggaat cgtcaataag
    33061 ctgaccaaac tgctggagaa agcttttact ccggaaaatc cgataatagc tccaccaata
    33121 cctccagaaa tacaagcggc aatgaatggt ttcttcaaag gtaatgtcac accatacaca
    33181 gttggttcag tgattccaaa taacgaagtc actgtagaag aaatccctaa tgctcgaagt
    33241 ttcgtatctt ttgttcgcaa tgctacagct aaagcagccc caccttgtgc caagatggct
    33301 ggaagaagca ttggcatcaa tacatcaaac ccatattgtt cgatattcaa aaacatgatc
    33361 ggtacgaatc cccaatgcat gccgaacatc acgaatactt gccataaact tcccatgatt
    33421 agacctgcta cgattggact aaatccataa atcgagttga agagaccgcc taaagcatta
    33481 ccggcaaccg tcccgattgg tcctaaagca agaaatgtta atggaaccat cactactaaa
    33541 acgacaagcg gaactaaaat catttgcaga aattgcggaa tcactttttt tacgaatggc
    33601 tctaatttac tttgaaccca tacggctaga atgattggta tgacagaaga agtataacca
    33661 gatggtgaaa gaatcactgg gatcccaaag aaactgatag cttctcccgc accagcaact
    33721 gctgtgatag ctggatacac aagtgccata gcaattgcta ctgctaaaaa ctggttggct
    33781 ttgaactttt tagctgctgt aaaagcaagc atgacaggta agaaggtaaa gacaccatct
    33841 gctgcagcaa acaatatttg ataagtaccg ctatcagctg tcagccagcc catcaccgtt
    33901 gccaatgata agaatccttt caataccccg gcggctgcca tcgcccctag aaaaggtgta
    33961 aatatactag agataatatc gatcagacga ttcaaaatat ttttggactc ttcttttcca
    34021 tcctccgaaa ctgcaagacc ttcctcatcc acgatcgatt gataaacatc tgctacatta
    34081 ctgccaatca ctacttgata ctgaccgcca ctttgtacga cttggatcac gtccgggtct
    34141 tcttttaact tttgcgtatc tgctaaagat tcatctttta atctgaatcg caaccgcgta
    34201 gcacaatgta ctaaactttt gacattcttt tgtcctccta ccgcttccca tacacgacga
    34261 ccaactgctt gattatccat tgttttttcc ttctttcttt tttatttaaa aaataaaaac
    34321 ccagattgaa aaaaacagtc acaaacattc gtgaacctat tttttcaatc caggttttgc
    34381 ctgccataca gtaacaatcc ttatgattgg atgctttttt tctcacttac tactctttga
    34441 atatgaatcg ttaaataaat ctgctctgcg atccccattt cctgtttttg cgtttgtcga
    34501 agatactcat tgatccgttc gacacaatga aacgcttttg gatactttcc ttgaactaat
    34561 gcgtagagaa attcatcttg ctcgtcagat ccttcccctt tgagataacg ctgggcaaaa
    34621 aactgcagat gagtaatgat acgctgataa ttcaaagatt cttcatcgaa cacgatgccg
    34681 aagaatttac tgatgatact cagtatatct cttaccattg tggtagcggt catcgtcact
    34741 tgcatgtctt cgtttccttg ttggctattc acaaaatgaa aggcaataaa accggcttca
    34801 tcctcagaaa gttccacacc cgtcttttgt ttcacgaaca aaagagcctc tttcgctgct
    34861 tgatattctt tcggataaaa tttttttgtt tcaaataata atggattagg cagttggata
    34921 ttttcttttg ctcgtgtgat cgcataatga agatgatcgg tcaatgtcag ataaatatta
    34981 ggcgaaaatt caacttttag tttatcttca gccagttcga ctacttgttt gacccattcg
    35041 atctcctctg ccggaatatc agccatcagc tgctggaatt ggtttttagc aaaagaatct
    35101 cttaaaacaa attctttttg gaccgcttct ggcgaaatct cgtccccaat ctttttttga
    35161 aaagctagtc cttttcccat acaaacgact tcttccccga atccattttt cgaaatgacg
    35221 acattgttat tcaaaatttt atcaattttc attttgcttc gccctctcaa gaaaaataaa
    35281 aaaacctaga attcgcctac tgtttcttag ctgaattctg gttttgcctg ccgtacagta
    35341 acaatccatt tcttattcaa aatcatcata ccatcgaatt tttatttatg caagcctttt
    35401 catataaaaa ataaaaaaac tggaacacaa gtctatctag aaaacaacca aatcagagat
    35461 gcacctcttt gcgtttctct ttttttcagc aaattggttt ttccttatcg attcatatcc
    35521 tttggctgtt tttaatctgt aacgacttat gtcccagtat cctaaaatgt ttttgtctct
    35581 agattgaaag cagatctcat atcatctgtc gcatctttta tcgatgtaac actttccagt
    35641 tctccttgta caactagtcg cgtgacggcg tccatgtcgc cacaagtaat tagtgtgaca
    35701 atttcttttc cctcgacttc attcagtaat tgggtatctg taggagcaac tttttcttta
    35761 aaaaatgttt tgtatgtata cacattttct aaatctgtca aatagatctt ggcacccatc
    35821 tctaaatttt ctagcggtgt aaacagcaat tcagggttcg ttgcacgatg gcttgctaag
    35881 gcataatttc cttttcccat ttcttgatct ggagataatg ttcctgctcc gtaaagtagt
    35941 gacgtattat ccaagccttt gaaaaccggt aaattgattg aaactgaagg gactgcgata
    36001 ctagcaatca ctggtagatc tgttcctctc atttgcgacc tcatgacccc ttctgtcgtc
    36061 attggttcta ccgcatcaaa gtcaaaactg acatctttat cgttattctt tttcagatct
    36121 tcttttgtta cattcgctat agcatattta tctcctgtct ctctgacaaa ataatccttg
    36181 atctgttcat tgaaaatcaa agctagtcct actaataaca agaggaacaa gaggatattg
    36241 actagccatc tttttgcttt tccttttttt ttcattttct ctccctccag actcatttta
    36301 ctgtttttct tttgctcttt ccaacagccg aatgacacat ttctacaact ctgtgtattt
    36361 ttttcgtaca cctgcgcttt tttctacagg atatttcaaa ttatttttga tcaatttttc
    36421 ttcaatgatc tcatctagat ccaggtgaag gttgtctgcc atcatgtatg aatagatcaa
    36481 tacatctgcc agttcttctt tgattcgttc taattgtgtt tcttttactt cttccggcgt
    36541 tttccattga aaaagttcta acaattcaga tgcttccaaa gaaatagaga tagccaaatc
    36601 tttttcgtta tgaaattgtc gccaattacg ttcatctcta aactggttga ctttttccat
    36661 actattcatt tgcttgcctt cttccaatca cttcttactt gattaatcat accatttttt
    36721 tacataaaaa atagatgatg cttttaaaaa aactcctgtt ttcttttatt taaaataaat
    36781 gtcatagaaa atcaataatt taggtagcca ccatgtgtat gcagtagcgt gtcttcttcc
    36841 cttgaggctt tttgtttttc gatacgttct tcttcccaaa cagacaacgc tactagaatc
    36901 tctcttaatg attttccttc ttcggtgagc gagtactcta ctttaggcgg gacttggtta
    36961 taggcttttc gatggatact ttcatcttgt tctaactctc ttaactgctg ggtcagcatt
    37021 ttttttgtga tcgtagggat ttgcttgctt ctccacgcct aaaataagcg atattgctct
    37081 tccctttatc tggtgtaagc aaagcagtta gcattttgac gatcgtgggt tccgcttttc
    37141 acttcttatc tacttagtat catgaagtat actttcgtac taatactaag tagcggaagt
    37201 gtcaactact ttgtttaact acatagcaaa agaaacagaa gggtgacaac ggtcatcacg
    37261 cttctgtttc ttttgaatat gctgtgttgt tgtctctatc tccgtgtaga ctttcctcaa
    37321 acttatcctt tgacagtaag tcagtaggct gatttgctct ttctagatgc cagatacatt
    37381 tctacaaaaa aatagtgatt ctgttttgaa aattcgattt ttagttctgc attttcgtaa
    37441 acatccaaca tttttttcgc actgtataga cctaacccgc catttttttt agaggttttt
    37501 cctcttttga ccatctccga tagcgtcaaa ttcgttggat ttgccttgtt cgctatacta
    37561 aaacgtgatc cctctttttc ttccagcaga ttgatcgtaa tcggactctg atcggctaca
    37621 cgatgatcga atgcgttaga aataaaaata gataaacagc gaataaaaac aaccagatca
    37681 atcgggattg ttttcacttc gacaggaata actacttgga tcgggatttt ctccgattct
    37741 gctttttcta gaaattttaa aaagacaccc ctgacagctg gggatacaat atttgttagc
    37801 tgttgtattt gcggtttttc aaaaatagct gcagaatgat cttctaatat ttctaaaaat
    37861 tccaacgttt cttttttatt ttcctgaaga gtaattttta atgaaaacag tagactttta
    37921 taatcatgtt tgaattcctc tagatgatgg taatattctt catttatttg atacagttga
    37981 agcgattcct gatgttgtct ttcttttact tggaaactga tcaacataat ggaaaaaaac
    38041 aaagaaaaaa aactgatgac tatcgacaaa agagatagaa gcagataatt agatgagcgg
    38101 aagtgaacac ttcccaatac caagtaaatc acgccccagc ttaataaaat acaaatacct
    38161 ccaaaatagt gttcagttgg ataagtaaaa agcaagttga gaaatctcgt ctgtttgtct
    38221 atacttttta ctaatagata gaaaataaat gcgataagta gtgcaaaaaa acttgctgtt
    38281 ccaagatgtc tctttcctaa ataatccatc agatcaaaaa ccagaaaaaa gctgagtcca
    38341 gtgatagcaa aaaaaagatt actgatcaaa cagctgataa acaggttttt cgtagtaaaa
    38401 taaaaaagaa acatcaataa taagccaatt aggtaaggaa agctcacttt agggatagga
    38461 agtaaaagtg tcaccaccat caaagctata caaccaaagc tgttttttac tagcagttgt
    38521 ttcgaaaaga ataaatagat acctaaatct aaaatcaaag taaaggtcaa ataaagtgtc
    38581 actgttgcga ccatcttatt cctccattgt tttgtttcaa aatgtacaca tattttttta
    38641 ataggagcct gcatatgaaa aagtatttta tcatagaaga taaccagttt cacagcgaat
    38701 ggatcatcag agaattggtc aatcatcata tcgagaatat cgtgcagctc acttctatct
    38761 ctcaaatcaa tcagttgatc cagttgggca ctattgataa tcacgatgtt ttctttatcg
    38821 atatcgactt gaagcaatca ctcacaggta ttgatgtcgc cgaacaaata cgtaaaaaaa
    38881 atgcacatag taatattgtt tttttcaccg cttatacgaa ttttgcagta gagatcatca
    38941 atcggaaaat ccttccgatt ggatatatca gaaaagacag gaatttatct gaacaaattc
    39001 gtcaagtgct agaacagatc aattggaaag aacagcagat cgtaaatgtc gataaaatct
    39061 ttattgatca aaaaggtgga agtctcgtcc tgtatcctcg ggaaatttta tatattgaaa
    39121 gtatcaaagg tttgaaaaac caagttatgg tcaaaacaac tacagaagat aaattagtga
    39181 atacttcttt aaaacaattg aagacagagt tagaaaagta tgcttacttt ttacttttaa
    39241 aatcttactg tatcaactta tcgcaattgc agaaaattga ttctgctcat tatgtgttag
    39301 aatttttcaa cggtgactcc ttactcgttg gtcgaaagat ctttgaaaag acaaaggaac
    39361 gtttccatga cttccaaaaa actgcttcga gttgatgaat aagagctgag acgtaagcat
    39421 agcagatttc agggagtggg ccttgaatcg gctccactcc ctgaagtcgt tgatatgatt
    39481 ttacgcttcc tcttctgtag gagcatctgt taacgaccat gtgaatgttg aaacgtagtt
    39541 gtctacagca taattggata caggaacaaa taattccact gattgtctcc cttgttccac
    39601 attctctcct aatgaataaa tccatgtgcc aatcccttca tttgctcctg cagttactag
    39661 atctactgcc tgatctgctg ggatttcgat tccttcagcg ccagttccag aaacggtaga
    39721 tggcattcca ctgctattag ttgcgctatt tcgaatcacg gcgtttttga atttcacctt
    39781 tgaaccagtt agtttattcg tatcagatgt tgtaccaata aattctgttc gtttgactga
    39841 taatttccaa ccggcataat ttccgctttt atccgttact tgtacgaaat tctgtacttg
    39901 tgtttgtgct ccagtactgt ccgttccgct ttgtaatgca gcatatactg tttttgctgt
    39961 acctaacttg attgttccaa aatcaagatc tggagcttgg tcaatcgtca gtaatccgga
    40021 tgttccctga tttggttcgt caggtgtaaa aggagtatca ggatcatttg gatcaacagg
    40081 aggaactgga acaacgtctt caggatttgc agcatctggt ttggtcacag aaatcgttcc
    40141 ttctgtagtg gctgagctct tattctcagc aaaaacactt tgtgtacata ataatagact
    40201 tccagctaga acggtactta atgcaattgt ttttttcatt tctttttcct tctttctttt
    40261 aaaataaata atccagcaaa taagccaata agaatgactc caacaagtat cagccatcgc
    40321 cagtcaaatg tttctggctg attttttaaa tcaatatcct tttcattcaa ttggtccgct
    40381 gcatcatcag ttatttcaaa atcggtatgg agattccagc gtttttcttt ccaagcaccc
    40441 ttcacaaccg cttgataatg tcccggagct aatttttttc cttctaatgg gacaagtaaa
    40501 tcaaaatttg tattgggtgc tacatctatt ttttcttttt ttattgaaag gtaaggctct
    40561 tttgtttctt ttttgtagat ttcaaccgat gtagtcaagc cgaacatata agaagcattt
    40621 ggattttgaa gattcgcctt aatcacattt cttccatcgc tttgatccgc ataagctttt
    40681 tttaatttca aatctgggga agactctttt tcgctatttc aaatcaagat tgctttcact
    40741 tgagaaaact gattgacgac accaactgta cttttgttag aaacgttctc ttttttttct
    40801 tgaaacgtga tccctcctgc gaccacacca gccagctctt tttctggcgc tttcagtgaa
    40861 agtgtctcag tgaccgcact ttgaggagga acagttattt ctcccactgt ttttgctaag
    40921 tttttcatat cataacttaa tgtttcatca aatttacttt ttgattctcc atattcgatc
    40981 acaccgtttg aattcgtcgt agcggagtgg atcaatgtct caactgttac tgatttatct
    41041 gttcggttaa agaccgtgta ggggatttcc tgtgttttcc caggctccat ccttacattt
    41101 atatatgttt tgttcttatc aatctgatta gccggtaatt caaaatctac cgcaaaattg
    41161 aattcggagg catggacagg tgtctgccaa aggatgaatc caaggagaag gatcataagt
    41221 aaagagcagt tgtttcgatg acttttattc catattcgat tcatcaatca tcctccttga
    41281 tctttattct gtcggcgcat ctgctaatgt ccatgttatc tctgctgagt aattaccagc
    41341 tatctgattt tctttggtta agacaagttt aaacgcccga ccgttattta aatattctga
    41401 ataatctgct tgcacattcg ttgaattttg tcctctgaca atttccagtt cttcttcatt
    41461 cactaattga tactcctctc ctttcttgaa atataggaca tcctgcatag aaaatcctgc
    41521 atcatttgtg aaaggctgag ataattttat cgttaagatc cacggatttt tcgtttctcg
    41581 ctgatcataa atccataatt tttcctgtat atccatttgg ccaaatgctt cttgtgtcaa
    41641 accagttgct ttgatctgac caaaatcgag tttggaaggg gcaagaaatg agagaccacc
    41701 ttctactacg atcttcgtag caggtgcaaa aggcggggca tcatgataaa caactggcgt
    41761 atcacttgga ttgtgattcc caatttcatc gttggtactt ggcttgttct gcacaccctt
    41821 agcagtagct tctcctgctt ggtcgtttaa tacaatttct aatacatctc ctgcaaaaag
    41881 ttgattttct gggatagaaa gaacaaattc ccctgtatca tcaatagttg tttctgacaa
    41941 aacagcttga ttttttttca atgaaaccaa ggctcctgct tcacctgtac ctgtgatcgt
    42001 gcgagaagct gcattgatca atgtggtact taaattagca ggttcaggag gaatcacatc
    42061 gactgtcgtt ttggcagagt ccagttctgt cacttcttgg ccacctctta cttttttagc
    42121 agcaacaact tgcacttgat cctccgtttt taatctctca tcattgggta cttgaacttc
    42181 gaacaagccg ccttgttcgc cttctcccca tgggttatat cctgcccctt cattttcatc
    42241 tgtttgtcct tgcgtgagag cagtaatttc tacagatgat ttcgtcgtat ctgcgtaagt
    42301 aatacgtaaa gtcacttcta cttcgcctgt ccaagcatct cttggctttg cctcgacacc
    42361 ttcgggaaca acgacatgcc catagatttt tttatctgca ttcgttggca ctcttaagga
    42421 ttcaatcatc gggagctgat tattggcact gattcgatta taatttttca ttccggcacc
    42481 tgtctgagca aattcatttg ttaaacctgc actagggctt gatacgaaat tgcctaagtc
    42541 ggtcccattg atcgataagt ctgtcatatc agaaaaataa tccgggtctt tttcaaagtt
    42601 atcgaatgtt gcgcctgttt ttcgccataa agcgatttct gaatgggcga tctttaaaga
    42661 agaatctcca tttccttgga atacccagcc gcctatgcca ttggtctcgc ttccaatccg
    42721 tttgttttgg aaatcaaaat aggtaggaga ttctaagtcc acattcaaca catccccact
    42781 gatgatgccg gtcttatctt cccctgtttt cccgaccatg actaaataag ttttttcacc
    42841 tgccgtaaaa tttaaatcaa tatctccagt tgtatctaca gttgctccgg aatctgcgcg
    42901 tatatcgata tttgattttt ctccagtgac tgtaaaatca gctgaacctg gactttctag
    42961 aaaaccagaa gcgtcataaa attggatcgc ttgatttcct tgattgagtc tgttcacgta
    43021 agatttgcca tcacctgtat ttcttaccct caatgagcct ccattggtta ctgtcagctt
    43081 ttgcgtacca ttctcaaaac gaactgcgct ggaacgccca ctctgcttat cgatgtcgat
    43141 aactgatccg gctccttcta cgatcaaaga aaggcctttt cctacaaaac gcatcccatt
    43201 gttcccgggt tgatcgtcat ctccagccat tttgaccgag atttgtccac cacttgatac
    43261 atggatttga ctagaatctc cttgcaagcg aaatccgccc gtagttgctg tattgatggt
    43321 gattttccca ccattcgata cagtgacttt cgatcctgaa cctctcattt ggatgattcc
    43381 gcgattgttg tcattggtaa tattgatctt tgtatttacc gaaaattcgc ttcccgaacc
    43441 actgacttca aacatgacat taccaggtag cgtatcgact gcctctcctt catcgctaac
    43501 gccatctgcc cgatcataat caagagagac ctttgaaccg cttaagatat ggattcctgg
    43561 cgctgttttt ccactaccag tctcatctcc tgaatctaga aaaccgtaga atttccctag
    43621 atacggtcca taaagttgac taccattgat cacattcaat tgatagcttc tacaaaaata
    43681 ggctttaccc ggagcggcat tcagtccttc cttcactggt tggacatcca acacaccgga
    43741 aacaccatct aatgtgacag ttgcacgagg agcgtaaatc agtcctaatt tggacccatc
    43801 atacaaatca agtgtcccgg taaattttat ttctcccgta aaatgatatc tgcttcccca
    43861 aaaatcggtc atataagcaa tgataatggc atttccatcg atatttggtg tcaatccacc
    43921 tggatgtgca gctgttcctt taaaattctg gaactcgatc gactgaggta tctcattgat
    43981 ataaatatgg tgtgtgccga aattgtgagc gaatccacca aaatcaataa cgatatctcg
    44041 tttgatctct aattccctgt ctaaagtcaa aggctgaatc agtcggatct tagttactga
    44101 ttcgtctgcg agaagctgcg ccagttcttc tgttgtggca gcttcgctgt acccatccgc
    44161 tgaaacagct cttgccagtg ccgcttcact gctatcagac gtttgttcag ctacttctga
    44221 ggtctcactt gtcttatttt cttcttgcct tttattagta gaagcctcac tttgagattc
    44281 tgtagtttca gaagtaatcg attcttctga aaaagtagtc ttgtcaaaac tttgattctc
    44341 tgattgcgtt gattcgtttg ttttctctac atcggcaaaa acggatgtca ccatcaagag
    44401 agggaagaca aacaaaatcg acgcaatcag cttttttaca cattgattca ttctccacca
    44461 tcctctctga cgttttgaca attttatttt atcgttttaa tctatttcca attgtacttt
    44521 catatttaaa atatgtattt ttatggaaaa atttttctat taggtgtaaa tttctttttt
    44581 gtacaaaaaa aaggctgtga caaaagtctt gtcacagtcc cttatcccga acaagcggtg
    44641 ttctcccgcc aactcctcgg tcacaacctc cattttaaca ataaacctgt aaatgtgagg
    44701 gcaatacttt gattgtcaca ggtaatttat ccccttcatc tccatcaaca ttcgtcgtca
    44761 attcttcctc atcagctaat gacacaacta attgctcaca agtaaggtag gctagatttt
    44821 tcgtagattc atctactcct tttaataggt ctggcaatgt tttaagcgta tcgatcaaag
    44881 aagaatcttt taaataaacc aaatggagct taccatcatc cacttttgca tttggaagta
    44941 atgtttcaaa accgccgact gagttagtta gtcctatcag caaggtactg ctctcaattt
    45001 cttccttctt tccatcaact tctacgtgaa acgagtagga ttgtgtggaa gctaactgtt
    45061 tgattcctga catgaaataa gctagttttc caaatttcgt cttcttctcc gcatctacat
    45121 cattgattga ttcagggata gagccaattg cgacgacatt cataaaatag tcgtcattta
    45181 tttttccaat atccaatggc ttgactgatt caatcgaaaa atgattgatc gcttcctcag
    45241 gttctagcgg tatccctaat gctcgagcca agtcattcac cgtacccaat gggaaaaatc
    45301 cgaaatttgg ccgatgttct tgttcagcga ttccactgat tccttcgttg actgttccat
    45361 ctccgcccat gacaaacaca ctatgatacc cttccgtagc cgcttcgcga gtaaagtttt
    45421 ttgcatcccc tgcttttttt gtatgcaaga cgaccacttc atcaaacacc gattccaatt
    45481 ttgctatcgc taattgctca aattcctttg cttgttcacc gcctgaactg gggtttacca
    45541 ctaataaaac ttttttcatc actttctttc ccttctgatt gatttcttta atataacgat
    45601 agcataaaga aaaaaattag gctgtgatga agtactgttc atgaaacatt catttatttt
    45661 ttgacaagat aaaacatcca tactatgaaa ctgaggaaaa ttaaaacaat gatataggaa
    45721 ataaaagaaa atgataggca ttgtatacct aataaagtaa aaatcttagg tggcagataa
    45781 aaacacaaaa atagcaagag aatgtaatag cgtttcttcc actttttgtt atcagacctt
    45841 atagcgatca gaggaatacc aaatgcataa ctgaaaaact cttgatgtac tgttaatatg
    45901 tcacggcctt gaataataaa tgcgacaatc cccgctataa ttagtgtgcc taacaccaaa
    45961 acggaaaatt gatgaatata ttttatatac aaatgatcca ttgtacttcc tgccttttta
    46021 tttgtgagaa aaacaaatcc gtatacgtga ataacttcta gaaataaaag aaatttgcaa
    46081 aaccactcca tttatctact tctacgtgca ttttttgata ttgttttctt tatttaagat
    46141 tatacagaaa aatatctagg gtcgctggat tctaagtaaa aaaaattttt aaaataaaaa
    46201 gaactaaatc aagtagtaat taa
//
LOCUS       DACDIG010000015        46048 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.015, whole genome shotgun sequence.
ACCESSION   DACDIG010000015 DACDIG010000000
VERSION     DACDIG010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.015"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            18..899
                     /locus_tag="IV247_000884"
     CDS             18..899
                     /locus_tag="IV247_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8776973.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            912..2522
                     /locus_tag="IV247_000885"
     CDS             912..2522
                     /locus_tag="IV247_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAP8776974.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2599..3258)
                     /locus_tag="IV247_000886"
     CDS             complement(2599..3258)
                     /locus_tag="IV247_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP8776975.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3541..4206)
                     /locus_tag="IV247_000887"
     CDS             complement(3541..4206)
                     /locus_tag="IV247_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8776976.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4353..4958)
                     /gene="dhaL"
                     /locus_tag="IV247_000888"
     CDS             complement(4353..4958)
                     /gene="dhaL"
                     /locus_tag="IV247_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAP8776977.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(4986..5969)
                     /gene="dhaK"
                     /locus_tag="IV247_000889"
     CDS             complement(4986..5969)
                     /gene="dhaK"
                     /locus_tag="IV247_000889"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAP8776978.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(5971..6366)
                     /gene="dhaM"
                     /locus_tag="IV247_000890"
     CDS             complement(5971..6366)
                     /gene="dhaM"
                     /locus_tag="IV247_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAP8776979.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            6520..6840
                     /locus_tag="IV247_000891"
     CDS             6520..6840
                     /locus_tag="IV247_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAP8776980.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(6952..7821)
                     /locus_tag="IV247_000892"
     CDS             complement(6952..7821)
                     /locus_tag="IV247_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8776981.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(7917..9221)
                     /locus_tag="IV247_000893"
     CDS             complement(7917..9221)
                     /locus_tag="IV247_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8776982.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(9218..9727)
                     /locus_tag="IV247_000894"
     CDS             complement(9218..9727)
                     /locus_tag="IV247_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8776983.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(9902..12037)
                     /locus_tag="IV247_000895"
     CDS             complement(9902..12037)
                     /locus_tag="IV247_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8776984.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(12120..13775)
                     /locus_tag="IV247_000896"
     CDS             complement(12120..13775)
                     /locus_tag="IV247_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP8776985.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(13777..14091)
                     /locus_tag="IV247_000897"
     CDS             complement(13777..14091)
                     /locus_tag="IV247_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP8776986.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            14261..14521
                     /locus_tag="IV247_000898"
     CDS             14261..14521
                     /locus_tag="IV247_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAP8776987.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            14535..14849
                     /locus_tag="IV247_000899"
     CDS             14535..14849
                     /locus_tag="IV247_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAP8776988.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(15579..16133)
                     /locus_tag="IV247_000900"
     CDS             complement(15579..16133)
                     /locus_tag="IV247_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAP8776989.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(16259..18188)
                     /locus_tag="IV247_000901"
                     /pseudo
     CDS             complement(16259..18188)
                     /locus_tag="IV247_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(18494..19210)
                     /locus_tag="IV247_000902"
     CDS             complement(18494..19210)
                     /locus_tag="IV247_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP8776990.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(19211..21034)
                     /gene="glpO"
                     /locus_tag="IV247_000903"
     CDS             complement(19211..21034)
                     /gene="glpO"
                     /locus_tag="IV247_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAP8776991.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(21048..22544)
                     /gene="glpK"
                     /locus_tag="IV247_000904"
     CDS             complement(21048..22544)
                     /gene="glpK"
                     /locus_tag="IV247_000904"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAP8776992.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(22680..24140)
                     /locus_tag="IV247_000905"
     CDS             complement(22680..24140)
                     /locus_tag="IV247_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8776993.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(24130..25425)
                     /locus_tag="IV247_000906"
     CDS             complement(24130..25425)
                     /locus_tag="IV247_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP8776994.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(25757..26434)
                     /locus_tag="IV247_000907"
     CDS             complement(25757..26434)
                     /locus_tag="IV247_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAP8776995.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(26464..27555)
                     /locus_tag="IV247_000908"
     CDS             complement(26464..27555)
                     /locus_tag="IV247_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAP8776996.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(27656..28090)
                     /locus_tag="IV247_000909"
     CDS             complement(27656..28090)
                     /locus_tag="IV247_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8776997.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(28674..30131)
                     /gene="ascB"
                     /locus_tag="IV247_000910"
     CDS             complement(28674..30131)
                     /gene="ascB"
                     /locus_tag="IV247_000910"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP8776998.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(30128..32023)
                     /locus_tag="IV247_000911"
     CDS             complement(30128..32023)
                     /locus_tag="IV247_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8776999.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(32081..33856)
                     /locus_tag="IV247_000912"
     CDS             complement(32081..33856)
                     /locus_tag="IV247_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8777000.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(33906..35402)
                     /locus_tag="IV247_000913"
     CDS             complement(33906..35402)
                     /locus_tag="IV247_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8777001.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(35596..37218)
                     /locus_tag="IV247_000914"
     CDS             complement(35596..37218)
                     /locus_tag="IV247_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP8777002.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(37546..38859)
                     /locus_tag="IV247_000915"
     CDS             complement(37546..38859)
                     /locus_tag="IV247_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP8777003.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(39104..39328)
                     /locus_tag="IV247_000916"
     CDS             complement(39104..39328)
                     /locus_tag="IV247_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777004.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(39348..39716)
                     /locus_tag="IV247_000917"
     CDS             complement(39348..39716)
                     /locus_tag="IV247_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP8777005.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(39912..40622)
                     /locus_tag="IV247_000918"
     CDS             complement(39912..40622)
                     /locus_tag="IV247_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777006.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(40640..41137)
                     /locus_tag="IV247_000919"
     CDS             complement(40640..41137)
                     /locus_tag="IV247_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8777007.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(41174..41626)
                     /locus_tag="IV247_000920"
     CDS             complement(41174..41626)
                     /locus_tag="IV247_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8777008.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(41761..42366)
                     /locus_tag="IV247_000921"
     CDS             complement(41761..42366)
                     /locus_tag="IV247_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP8777009.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(42816..43436)
                     /locus_tag="IV247_000922"
     CDS             complement(42816..43436)
                     /locus_tag="IV247_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8777010.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(43438..44307)
                     /locus_tag="IV247_000923"
     CDS             complement(43438..44307)
                     /locus_tag="IV247_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAP8777011.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(44330..44737)
                     /locus_tag="IV247_000924"
     CDS             complement(44330..44737)
                     /locus_tag="IV247_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP8777012.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(44756..46045)
                     /locus_tag="IV247_000925"
     CDS             complement(44756..46045)
                     /locus_tag="IV247_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8777013.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT        12919 a         9838 c         7886 g        15405 t
ORIGIN      
        1 aaaggagtgg ggtttttatg gcagaaatcg tcaaagtcac acatttgaaa aagagctttg
       61 gtcgtttcga agctttaaaa gatgtctctt tcaccgtaca ttcaggtgaa gtcgttggat
      121 ttatcggacc aaatggttct ggaaaatcca cgacgattcg gacactttta gggattataa
      181 aaaaagacgg cggaacggca acgatttttg ggcaagatgt atgggaagat agtttatcta
      241 tccatgaacg tctttcttat gttccaggcg atatctcttt gtggggaaat ttgactggag
      301 gagcgatcat cgatctcttc atgaagcttc atggaaaagg gagtgctcaa aaaaaagatg
      361 aactgattca acgtttctct cttgatccta agaaaaaagc aaaaaactat tcaaaaggaa
      421 atcgccaaaa agtgggcttg attgctgctt tggctgttga ttcggatctc tatttatttg
      481 atgagcctac ttctggttta gatcctttga tggaaacgat tttccaagaa gaagttgaaa
      541 aattgaaaga acagggcaaa tccatcctac tttcttctca tattctcagt gaagtcgagc
      601 gactagcgga taaagtcgtg atcatacgcg aaggaacact cgatgaagaa ggaacactcg
      661 atgaattgcg tcatttgact cgttcgacca tcactataga aacagaagca gagaatagtc
      721 acttagcaca attgaacggt gtccatgact ttgtgcaaaa aggtcacctc gctcaatttg
      781 ctgctgataa cgaagctttc gatgtgattt tagctgaagc gtccaaatta ggtgtgaaaa
      841 aatttgaatc tattccaccg actcttgagg atttattcat gagacattat gaaggatgag
      901 gaggaagcga aatgactgat aaattttcac gctggtccac ccttttttct caaaacctta
      961 gccgtgactg gaaaaagatc ctgatatggc tagtcggtgt cactgctttt tctggtggtt
     1021 ttgtccctgc gtttgaagaa ttgacttccg gacaaggatt agctggatta tacgaaacct
     1081 tgcaaaaccc ggcaatgatt gcaatggtcg gaccaactcc agcttcgaat gctgctgagt
     1141 atactttagg agcgatgtat gcaaatgaaa tgttgctttt ttgcagtttg tttgctgctg
     1201 tgatctctgt tttacacgtg gtgagccaaa cgagaaaaaa agaagaactt ggtctgattg
     1261 aacttattcg ttcctttcga gtcggacgtc aagcaaactc tttagctgtc atcgctgaaa
     1321 gtattctgat caatttgctt ttagcaatat cgatcagttg tacgatgatt tttttccaag
     1381 cggattcgat cactgcagaa ggtgcttggc tttttgggct atctgtaggt agtatgggga
     1441 ttttggggag tgccgttgca ctgtttgctg cacaactgat gccaagttca gccagttcga
     1501 atggtctgtc tttagcgttt ttaggtggac tttaccttct acgagcaata acagatgtag
     1561 ctgattcctc cctttcgatg tggaatcctt taggctggtc ttatttgacc tatccattta
     1621 caactaacaa ctggattcct ttgctttatt tgtttttgtt cagtctaatc ttgataatca
     1681 gttcatttgt tttagaggaa catcgagatt tgaacaccgg ctatatcccc gaacgtgaag
     1741 gaaaaccgta tgctaaacgt tctcttttat ccttgcgtgg tttattattc tatctaaata
     1801 aagggatgac aatcagttgg cttatcactt ttggtttgat gggggcggca tacggctcta
     1861 tctacgggaa tatgcaacag tttttagaaa gtaacgactt aatgaaacag atgtttatcc
     1921 aatctggcgt ctcaattgaa aaaaacttta caagcacgat catggtcgtc ttgatttgtc
     1981 tagctgctat tttaccaatt gctagcatca atcgtttatt tacagaagaa acccacggac
     2041 gtatgaatca gctgttcgca acaaaaattt ctaggaacca actttattgg accaatatgt
     2101 tactagctat tctatttgct atattggcaa tcctgattag ttctggtgca ctagggcttt
     2161 ctgctttggc ttcgatgaag gatgcgaaag acatggatct attgatgttt cttaaagccg
     2221 ggttcaattt cctgcctgcc gttttagtgt ttatcggaat tagtggcttt ctattcggat
     2281 ggcttcctcg aatcggtaaa cttgcttata tctacttagc ttactccttt gcactcaatt
     2341 actttggcgg aattttggat ttaccagaat ggttttccaa aacagctgtc cctagctggg
     2401 ttccacggat gccaaacgat tcttttgatc tttccgtgtt ccttacgtta tcatgtatca
     2461 gccttgcact gttcgtcctt ggttctattg gttataggcg aagagactta ctagaaggat
     2521 aataaaaaag catgtacaat ttcatgctag cctccaaatc tgggtcaaaa atccaacatt
     2581 tggaggtcat ttttttattt acaagtcttc taactgctct ttatcaatga tccagacttc
     2641 tcgattcttt ctttttacat aaccattctc ctctaataat ttaaattttc tagataaagt
     2701 ttcgggggtc gtaccgataa atgcagcaag ttctttcatt ttcattggca ggaaaaagtg
     2761 atccttccct gtcgctttag ctaagtcaag cagatagaca gcgactcgtt cttctacttt
     2821 ttccatcatc aaaaaagttg tctgctgctg tacttttgtt aatttctcaa cgttgattgc
     2881 taaaagtttc agactaaggg cgggataatg cagtaagagt tccgagaaat ctttttgtct
     2941 caatatgcaa atttccgttt tttctagtgc ttcgccaaat aaagactcat ttggaacgcc
     3001 tagcaactgg ctctctccct catatccgcc cggctctatt actcgtaaaa gctgttcctt
     3061 tccgctagca gataattgat acactttcat actgccggag gccacgatca ctaattgagg
     3121 ttctccattc ggcgcaagaa tctgttctcc tttttccaca tagtgatgtt tgacaagtgc
     3181 gtgtactttt tcttgatctt gctgtactaa gtgattgaac aaaggaacaa atgaaacaca
     3241 taaatgatca tgactcataa tctctcctcc ttctaaacag ctatttctaa taaagaaaca
     3301 taacttccct tattccaact gataattttc ttttatcata gcataaacgt caagcgaacc
     3361 agataccagc aaatagagtg gatttctttt tgtcccttgc tttattttcc ttcttttgag
     3421 accttaagta attgattcct atcaattttt taaatattta ctttttgaat aatcaaaaac
     3481 aaaccttcac tcgcaagccg agagtaaggt ttgtttttcg cattgcttat ttcgtgttgt
     3541 ctattgaaat ttttccgcac ctagcttttg atatagtggt gctagttctt gtaaggtttc
     3601 agcggttatt tgctcaacgt gttcgatttc ttgcggatag aagaatagtt tacccatttg
     3661 aaaggttcgg tctctcatcg cttttttcaa ctcttcttgt ttctttgtat cggacaaata
     3721 gtcagacaaa gctaagtggt cgtcaagttc atgttctggt tgcggccata tgtaataatc
     3781 cttgatatcg actgttttgc tccactcttt cagtatcgca agcaactggt tatacgctgc
     3841 aatgctacca tatcgttctt cgcttttata atgttggtac atcaaataga tttggtattg
     3901 tttttgattc aacaaaactg ctagacaggc attctcgtct tgacgagatt cgcttcggta
     3961 cgcgctccaa aaatgactgc gtaaattcca gccattcgtc cagctttcga ttttaggttt
     4021 gctcatgccg tacatatcag gcaaaagtgc agctgtctgg agctgaatct ctttccattt
     4081 ttgccagtgt tctttatatt ctttcttgat ttgttcaatc ttatctgttg gatggctctc
     4141 tttgagctgt ttgaatgcgt agattggtcg atcaaacaac tgataatctg attttccaag
     4201 ttccattgtt tcgcctcttt ccgttatctt ttgtattcta cctgaatttt gcgatatgga
     4261 caaatagagg ttgtgaaaaa gctgttctgc atcaagtcat aagaacagcc aaacaaaaaa
     4321 ctgcgatgag catcaccgca gttttattga acttaaggtt gattgatcac atctaaaagc
     4381 gttgaaaata aaatggcact agaagcagct cctgggtcga tatgtccgat tgcacgctct
     4441 cctagatagg aagctcgtcc ttttttagct accaagactt tggtgtgttc ttttgcctgg
     4501 tcaatgattt cttttgttaa attccctgcc ttcaatgcct ctgtgactgg cagccaaaca
     4561 tctaacatcg ttttatcgtc agcttctgcc tttcctcggc tttgaatacc cgctgttccc
     4621 tgctcgatca cttcgcctaa tttttcttga gaatcaatag aatcgacctc ttttgtcgct
     4681 ttcatcatgt tcatgaatgc agatccgtat aacggaccag aagccccacc gactttcgat
     4741 atcatcgcca tagagaatac tttgaatgta tcgctgattg tttcaggatg atcggcttga
     4801 aaagcttctt cataagctga aacccctctt cccatgttat ttccatggtc tccatcgcca
     4861 atcggggtat ctagatcact gagatattgt ttgttctgat tgattgcctc tgcaaattgc
     4921 gtgagccatt cttggatttc ttgaacggtt agtttcatat tatcaccctt ttatcttgag
     4981 attttttacc atgaaatcgt ctgtacattg ctttctaaag aatctttcca ttgtgtctcc
     5041 gtcaagtcga tcagtgttag agaaagacct tgcatatcta gtgaggtcat aaaattaccg
     5101 acttttcgga aaactacatt gacgcctctt tcttccaaca gagctaaaac atcattcatg
     5161 aacacgaact gttccatcaa aggcgtcccg cccattccgt tgactagtac acctgcttct
     5221 tttggtaaat tcgagtaatc atctaaaatc ttagtaacaa gttcctttgc cagtacttta
     5281 gaaggctgca tcttttctcg acgatagcct ggctcaccgt ggataccaac accatactcg
     5341 atctcatctt ctcctagttc gaatcctggt ttccctactt ctggtacagt tgctgctcga
     5401 agcgctagac cgatcgtctt agttgcctgt acgacctttt ctcccagttc tttcaactca
     5461 gctagggaag caccgtttct tgctgcatcg cccacgattt tgtgtacaag gacagtacct
     5521 gcaactccgc gttttccagc agtatacgta ctattttcaa cggcgatatc atcatctacg
     5581 atcacacttt ctacttcaat atcgtccata gcagctaatt cctttgccat atcgaagttc
     5641 aatgcatccc ctgtataatt cttaacgatc agcaaaactc cttttccact atcggcttcc
     5701 ttaatagccg tctgaatctg atctggagtt ggagaagtaa acacatcacc aagcaccgct
     5761 gcacttaaca tcccttcccc tacaaacccg gcatgggatg gttcatgtcc gcttcctcca
     5821 ccagaaacca aaccaacttg ttccgctttc tgattcttgg ctacaacacg ggaagattct
     5881 acacgatgaa caagttctgg atagcttttt actaatcctt ccagcatttc ttcgacaata
     5941 ttgcttggat cattcatgat ttttttcaat ttattcccat cctttctttc cggcattttg
     6001 tgcttctgtt aagatttgct gagtatcatc tgttataccg gcagtgatgg ctgctgcaaa
     6061 agctccttcc actaaaggcg catccaccag atgataacgt tgctgctgct cttcttccaa
     6121 catgtctttt gccagttccg agttcaatac cgcgctaccg atatccgcaa aaacaaaata
     6181 actagctgca tcgcttgatt cattgactgc ttctacgatt ttcatcgggt ctgatccgat
     6241 ctcgccattt attccgccga gagaaaaaat ttgcacctct tcggattttt gcatctgctc
     6301 aatcatttct ttaataccat ctgttacttt ttgactatga gaaataagca aaatactttt
     6361 tttcacaaga atcttccttt cttttacata gttgctaacg attggtttcg ctttcattaa
     6421 tgaacaattc aagtatataa taaatttcta attttgggaa aatatgtgta gtgcttcttg
     6481 aagtttgtta tactttaatt aaaagagagg agtgatcgaa tgattacaac aacaacgaac
     6541 agtgtagagg gaaaagccgt agtagcttat aaagggatcg tttttggtga ggtcattact
     6601 gggatccatg catttaaaga cttggaggcg gggttgcgaa atatctttgg cggccgctca
     6661 aaaagctatg aaaacgaact aatgggtgca agagaagaag cacttgccga aatggccgaa
     6721 cgtgcaaaag cattaggagc tgatgcgatc attggcgtaa aaatggacta tgaagtatta
     6781 ggcagcgaca atggcatgct gatggtcaca tgtagtggaa cagctgtctc cacgagataa
     6841 gtaggggaca tctgttttaa taactaaaga gagatgggac atgtagtggc actcaagaat
     6901 tagatttttc agtctaactt cgaggtactc taccttccca tcctctcttt tttatttctt
     6961 tttgcggatg attccgtaag ctgctgcgac taacacaccc gcccctgcta aggaataact
     7021 gacagaagct gttttttcat ttgtttttgg caaagtctga cttgttgatt tggatggctc
     7081 tgtcgtttgc tgaactgttt ctgtcgtgct tccagaggtc actgattttt ctcctgccga
     7141 atgcaaaata tcttttattc gttggaaact agctgctaag tcagcttttt ttgcttcaaa
     7201 atcattagca actgtttgtg cttcgctgat ctttccgtgg attgcttgac tggccgctgt
     7261 gatatccgct tgagtttgac ttagctttcc agaagctgtt tgagcaacct gatttaattg
     7321 ctgattgaat tcttttgctt gttggatctt cccgtcaaga tctgtttgga cagcttccgc
     7381 ttttgctttc gcttcggcaa gctttttctc aagttgtgca cggtcatttc ccactttatt
     7441 tttaagatca gctaaatctt gatcgattgc tgcttggtct tctttcaatt tgttttttgc
     7501 agcttctaat gcttcttcga gttcttttct tttattttcc aagtcttgca aatgatcgat
     7561 ttggtcagaa gaggtagaac tatttattga tgtatcttct gaagatgaaa tcgtttctcc
     7621 tgaattatct gtcggtacat tggaagtcat ttctgtttcc gaggcaccgt tttcagcacc
     7681 agaagagctt atggttgttg aaggttctgt ttgagcaggt tcagaagttt ccgcaaaagc
     7741 tgtctgtccc aataagaaaa tactggaaac agcaattggc cagattaacg gcttcattct
     7801 ttttttcatc acttttccca tccttactac tttttaacta atttcttatt ataagcaaaa
     7861 atgtttggaa gaaccataaa cacaacaggc tgtttgtcta aaaaacggtg catcttttat
     7921 ttttttgtac gttccgggct tttccatgtc ccgcttccat aacctaaaat cgttttgact
     7981 gctggaatga aagtcgtcac gatcgttatc gcgttcacta tccagaacaa aagcatatat
     8041 agtggtgcga aaagaaggta tttcaatttt cttcgtcgat catctacaat cagcgaagct
     8101 agcaactgga aaaatcctgc gaccatttca aagcagatga acacaaaact gaatgtaaaa
     8161 gtaatataaa tctgttcgta gtttccttga tatacataat gcccgattaa tccagcaaaa
     8221 agaactacgg aaatacaaaa gaatattgac cagacaatac tcaatgtttg atcaatgaag
     8281 atgatcgttc ttccaatatg tttaaaagga tggagcatca cttttttgaa attcgtcagc
     8341 cagacttctg ttccaccttt tgcccaacgt tttctgtgtc gatatagcat cttcaacgtc
     8401 acaggtactt ccatgaaaaa gataatctca ggagcaaaaa cagataacca gtcatttaac
     8461 tgatgatccc aagcaatact gatgtcttct gtcgctcgat cttgtctgaa tccgcccaca
     8521 tcgatcaagg cttcttttcg atacatcgta tttgctccac tgtacgcata aagaccaccg
     8581 aaaacagctg actgtgttcg tttaatgatg cccacgatac ttgagaattc aaccgtttga
     8641 gattttgcaa tcaatttggt acgattttgg acatccatat ttgcagtgac cgcagcaata
     8701 tgtcttgcac cagggcgaat gaaataattc acatacctaa tcagtgcatc tggctccggt
     8761 accgtgtctg catcattgct taaaatcaat ttcccttttg caaaggcaag tccgatatta
     8821 aacgcgtggg cttttccttt atttttttcg atccgaacca cacgaagatt agcgtatttt
     8881 ttcatcagtc tagctaatat ttctggtgtt tggtccgttg aaccatcgtc tgtcactaat
     8941 acctcatagt tgtgataatt caatttcgtc atcaaatact cgattgtata ttcgatcaca
     9001 atttcttcat tatgggctgg caccatgatc gtgatgaacg gttcgacact caagggaaca
     9061 tctacccatt ctttttgctt atgtttgtat aaaaacacat aacaccagac accaataaac
     9121 catacaaatc cgcctaaaat cggataagaa acgagaaaaa aatacataat agacaaacaa
     9181 gtatctaaga ataaattacc tgtatgggtg attgagatca tttcagttcc accccatttt
     9241 ctttatacaa atctgcaatg aatgtcgtat ctagattttt ctcttcggag acagaataaa
     9301 aacggaccgt ttctctctct tcttttgttc caaaacgttc cgtatacact tcttcaagta
     9361 attcttgtct ttttttcaat ttttcatctt catattcaaa agatttattt acttttttgt
     9421 atagatttcg attgttccac ctagtcaata caaatgaggt aatcaggaaa ctgaaaaaaa
     9481 tcaatacaaa aaagccgatt aagaaataca gcattttctt ttctgataaa aaagcgtaca
     9541 aggaaaaatc cttggctctt tctggtctcg ttaatgggaa aaagacccat ataaaaggaa
     9601 gaacaacgat catccatcct aaaagagcca ctaacgtttg ccaaatcttt aaccagtaat
     9661 taccttttga aaaatactta tctttcacca aaattgatgc aacttcttct cttgttactt
     9721 ttttcatatc ctcacctcac gtttttttgt tacgacgtag gagtttacca tacatttcat
     9781 ttcttttcac ataaagtgtt cctaaatttt tcctaagaaa aagaacaaaa catttctcat
     9841 cagcaaatga aaaatatttt gttcttttta tcagttgttc tttttatttt ttatctctta
     9901 attatgttgc tctttccaat acgacaatgc ttttcattcc tctagatatc gccgtgtaca
     9961 tttgtctgcg tcttctttgc tcatctccat cgttttcaaa tggatataag attacatgat
    10021 caaattccag tccttttgct agactcaaag gaatgatttg gcagttttct aatccatatg
    10081 aatttttttt aatcgattct tgtagcgttt gggtttcctc ttctgtttgg gtgataactg
    10141 ccgctttttc tccttttaga gaaaccaaaa tctcagctag tattttcaac aattcttcag
    10201 aggatcgaaa agagcggtat tcgggttctt ttccttgcgg tcggatcgat acgatgtcga
    10261 tcttctcttg atccacagca taggttttga aaagctctgt gattgctccg ctagaacggt
    10321 aactatttct aagatcatac cgatggatcg gcagatggta gtcgtcgaaa caacgcataa
    10381 tatcagcaaa agtagtggaa gaattaaaga ttgcctgatt ctcgtcacct actaaagtaa
    10441 agtgagtttt tggaaataat tcgatcaata gacctaattg tgccagggta taatcttgta
    10501 cttcatcaat caatagatag cgaaatgctg gtacatttac cttttcaaca agtaaatgac
    10561 gaatagctaa tataatgact gcttcatcaa cagtacgtgt gggttgccaa gcataagctg
    10621 agccatatcg tttttcataa attctttcaa acaactgatg ctcatccacc catgccattt
    10681 cttcgatttg tctggttatt tttcgatatt ttttttgtaa aagtttcctt gcataagcta
    10741 caatactttg ttccgaatca tcagaaatca atttaccgaa taattccaac tgcctatctt
    10801 ctgtcaacga gagtacctga ttctgcacag caggactttt tgcttgtttc aaaagatgat
    10861 tcttccattc gctcatcagt gcctttttcg ttgcttgaag cctatcgatc aatttaggat
    10921 gagcaggaac tttctcatat attttttcga tcgttttttt agaaaggact gtttttccat
    10981 ttttagtaag tccacggata aaattcggat gatctaaaaa caaaggatct gattgtttta
    11041 gattatcgat aaactttttg ctacgcaaac gttctgtctg ctccgataca ttttctcccg
    11101 tgatacgttt gaaataagcc tcttcacctt ctatttcctc tgctgaaagc tgtgaaacca
    11161 gctgtatgat ggttagattc agtggatttc tttcacctaa tgatggtagg acattcgaaa
    11221 tatagtcgat aaaccgatga ttaggtgata gaatcagcaa gtcatcggat gtcatcgtat
    11281 ctcgatataa atagagcaga tacgcaatac gctgcatgat agcagaggtt ttaccacttc
    11341 ctgctacgcc atttaccaag atattttgac ttttcacatc ccgaatgatt gtgttttgtt
    11401 ccgattggat cgtagcagtg atagccttca tttcattggt ctcatcttgg cctaagactt
    11461 ctaataatac gtcatcttga atagctacag acgtatcaaa aaacttgacc aaacgatctc
    11521 tttccaaaag aaattgtcgt ctttctttaa taaccgtatc gattttattt tgacgaaccg
    11581 tataagaaga tggaccaatc atgttattgt aaaaaagttc ggcaattggt gaacgccaat
    11641 cataaacaag attttcatct gcatcatctg tgaaatttgc tgtaccgata taaaaggatt
    11701 cttttggctc attatccaga aagtctacaa cgacttttcc aaagtaggga cttttcaata
    11761 aacgttcgat ctttgctagc tgattttctg cgtttttctg tttgatattc atctgatcga
    11821 tttctctatt tttcatttca atcatcgaaa aagtatccag gttatctaag taactgtcaa
    11881 aattcaatcg aagctcgcca gccatttctt tcaacgactg gtttccttct atttttgccg
    11941 tatccatcga ttcccgcagt tcgttttttt tatcaattag cttttgatac acgttcttca
    12001 gatatgcttc ttctaactgt ttttgtgtgt tgaccataca cattctcctc tttttttaaa
    12061 gtggtggaac cgattagttt acctctttta agagtcgaat gcaagctttt taacgagtat
    12121 catttgttag gatatatcag ttcttcaggt cggacagttt gatataaaga aaatgcacca
    12181 tcaaggtttt ttacgtggta gccagactgc ttcaagattc tttccgctag atagctacgt
    12241 aatccgctat gacagctgac gatatattct tgttggctgt ctaattcatt taatcggtcc
    12301 cgaagttcat tcaaagggat atgtttggct tccttgaatt tgcccttttc caattcttct
    12361 gctgttcgca catctaataa aatttttcct tttgctagtt ctgtcggcaa ttcatgccat
    12421 tgtacggttt cactaagccc ttctgcaatg ttcattgctg catatcctag catgtttact
    12481 ggatctttgg ctgagccaaa tggcggtgca tacgtcaatt ctaattccgg taaatcgaag
    12541 atcgtcaaat gccctttgat tgctgttgct aaaatatcga tccgtttatc cacacctttt
    12601 gccccaacac cttgtgcacc ataaatctcg ccagtctctg gatggaagat caacttcagt
    12661 aaaatatctg ttgcttctgg ataatagctt gcatgatctt ttcctgatac atgaatgact
    12721 tcaaaagaca gttcgttttc tttggctgtt ctttcactta aaccggtgga tgcggcagtc
    12781 attccaaacg ttcgtataat tgctgttcca ataccgcctt gatttcttcg agaaattccg
    12841 gaaatcgtgt ccgcaacttg tctaccttga cgattggctg gtgaagcaag agaaataagt
    12901 gcatcttttc cagtgatttg ctgttttaca acgatagcat cccccaccgc ataaatatca
    12961 ggaatattcg tttgataacg ttcgtctact aggatacccc cgcgtatgcc taattcgatt
    13021 ccagcggtca ctgctagctg gttatcagga cgaacaccaa tggataaaat aactaaatca
    13081 gcagggatgg tacgcccatc atccagtcgc aatcgtttcc cgtggtcttc aaatccagcg
    13141 acagcatgag aagtaatcac ttctactcct tgatgagaca attctttttc tataaaagct
    13201 gccatttctt catccagtgt tggaagaata tgtggtgctt gttcgatcac catgacttct
    13261 aaacctcttc tttttaggtt ttctgccatt tccaatccga tgaatcctgc accaacgatc
    13321 acggcacgct ttggctgttt ttctaatgca tgcatcactt tatcgatatc tggtacattt
    13381 ctaatagaaa aaacattgtc ggcttctgtt atccctggaa tcgatggaac aactggtttt
    13441 gcaccaggag ataaaaccaa tgcatcgtac tgttcaatgt gtttttgacc ctcatgtacg
    13501 acttctaccg ttttgttttc gggaaagatg gctgtcactt catgatttgg acgcacatct
    13561 aaacaaaaac gagcggataa tgattctggc gtttggacaa gaagattttc ccgttcactg
    13621 atttctcccg aaagataata aggcaagccg caatttgcaa aagaaacata cggtcctttt
    13681 tcaaaaacaa cgatttctgc atcttccatt aatcggcgaa gccgtgtagc agcagacatt
    13741 ccacctgcta cacctccgac aataacgatt ttcatcttat ttccctccta ctgttcgatc
    13801 aaaccattga ttcatgccac cacgtacatt cactacatca taaccagatt tctttaattc
    13861 tttagctgct tgtttactcc tcatccctga ttgacaaatc acatagacgg tcttttcttt
    13921 gtctccatca tatctgttta tactttgtaa tggaacattt ttagctcctt ttatatgccc
    13981 gccccggtat tctgcaggtg ttctaacatc aagtaacgct ggattttcca aaagcttttc
    14041 ctttaattct gggactgtta tcgattttat tgattgaaat agcaaattca ttttctgcac
    14101 ctcatttaca tttttataaa tacctcatgg ggtatattaa acaggagctt tattaatatg
    14161 tcaaccaaaa tgactcttca tttttttcac tactttttat atttgcataa aataccgata
    14221 aggggtatta tctaattata aaaatagagg agaatgtgtt atggagattg ataataaaaa
    14281 tatcctgaac cgtttgaaac gtacagaggg acaaattcgt ggtatccaaa aaatgatcga
    14341 agacgaaaaa gaatgtatgg atgtcatcac tcaattaagc gctgttcgtt ctagtattga
    14401 tcgcgtaatg ggcatgatcg ttgcagataa tctgaaaaat tgctttgaga aacctgatag
    14461 taatccagaa gaacaagcgg ctaaactaga acaagcgatc aagatgatta ttaaaaagta
    14521 aaggaatgat ttcaatgaaa gtcgttttcc atattgatga attagaaaaa tggtcagaaa
    14581 cagggaaaaa cgtaaaaaac ctgataaaag cttctccaga aacgacgatt gtcgtatctg
    14641 taaatggcat cgcgattacc ggttatctag actctgccaa tgcagaattc ttagatttgc
    14701 aaggagtcgt ttttcacgct tgcgccaatg ctatgcgtgc taatcatatt tcagaaagtt
    14761 ctttacctga acaagtaatc gttgtaccag caggtgtcct tgatttagtt gaactgcaat
    14821 cccaaggtta tgcttatatc aagccttagt cagtagaggt tgcgacagaa gtgatcagct
    14881 tcaagaaatt tcggcttatt tttcgaagaa gctacttctg gaaaaccgtg aatcaaaggt
    14941 tgtgaaaacg ctgtcctgca tcaagtaata agaacagcca aacaaaaata gcttctcata
    15001 tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttaaggaag
    15061 aggttgtgaa atcagcggtt tgacctaagt attagagaaa aaaacgggaa aatagctcct
    15121 catattttgc cgttttttga gctaataccg aaggtcagct gattgaacac cgtttaagga
    15181 agagattgtg aaaaagtcgc ttagtttcga gtattaagaa aaattttgtg aaaatggatt
    15241 tctacatttt tgcataaatt tggcttaata ccgagaaact ggcttttgaa taccgtttaa
    15301 ggaagaggtt gtaaaatcag cggtttgacc tgagtattag agaaacaaac ggaaaaatag
    15361 ctgctcatat tttgccgttt tttgagctaa taccgaaggt cagctgattg aacaccgttt
    15421 accaagaggt tgtgagccta ccatttaact ccgaatcaca agaaataatt tcaaaaacaa
    15481 cttctcctgt ttttggttcg gttggcttat tatcgagaag tagctactgg gagtctattt
    15541 atcaaaaaaa ggaatgcgac ataggtcaca cccctttttt attttctttg aaacatcttt
    15601 cggtactcga cagcatacaa gacaaaagat tgtaaaagac cgtaagtgat gacggcaaaa
    15661 atgactctca cccacagcgt tggtaattct gggaaaagca gcaaaaggat catgccaaca
    15721 tagatgacag cagtcgctac tttgccatac cacttcgctc cacttaattt gatattttta
    15781 gatagcccga ttaatccagc tacaaacata aagccttctt ttatcaagaa tagaccaaac
    15841 aacagccaaa gccacggtcg ataccatgcc atagacaaaa tcagaaaaag ttgtgtcaat
    15901 ttatctgcaa acggatcaag caccttccct aagtctgtta cctgattata tctccgagca
    15961 atgaccccgt ctagataatc agacaaacct gagacaacaa tgaccgctcc tgctatataa
    16021 aatgactgtc tcagcacaaa ataaagataa atcggcagca aaaaaatacg gaatatcgat
    16081 aaaagattcg gaatcgtttg tatttcttta cgccagtctg tgcttaattt cattttatcg
    16141 ccctacctcc tcagcactct tttcttttta ttttagctta cacggggaat aaaaccaatg
    16201 atttcactgt ttttattttt ctttacagac actgatatta tgagctaatt tgagataatc
    16261 acttgctgtt ttcatcgttt actcttcttt cttcctgtcg tttgcgcatt tcttttacta
    16321 gtactttatg agcttttttt gatcctcgct ctaggtcacg ctgccgttta cgtgttttat
    16381 aatcgataaa cagactgtct aaatcgtaac ctgtcgggta cagatcttct gctgaacgca
    16441 ccagacgaac acgctgacga ggcacgtgga ttattttttt gtctaaatat acatcaatcg
    16501 tatcttcacc tatatcttca taaactaacg cttctttttg gtattcattg acaaatatcc
    16561 gatcaccttt tgcaaagagc tgacgttctt ttttattctc cttcctattt gtaactgctg
    16621 tcttaggaag aaattctttt ttctgtgtcc gataggcttt ttccgccaag taacgctctg
    16681 cttgatggat caatttcatc gacatatcca ttttatgagc aatccacaaa gcttggcttt
    16741 ctcctgtttc ttccaacccg caactgatat ttaggacgaa gtgcttcccg atcgaaagcc
    16801 atagcagccg gcacaaaatc ttcatgatct ccggcgaatt tcttgatttc tccataatgg
    16861 gtcgtggcaa tgatcaatgc cccttgctca tacatagatt ccatgattgc gatcgctagt
    16921 gcagcccctt catttggctc tgttccactg ccaatctcat ctaaaagtac caacgtattc
    16981 ctttttatct ttttcaaaat agcagcgatg ttttgcatat gaccagaaaa tgtactcaac
    17041 gcattttcta aattttggtg gtcaccgaca tcgacgaaaa tctcatcaaa gacagcaagc
    17101 tctgtccctt cttttgccgg aacttgcata ccgaacatcg ccatcaaagt caataaaccg
    17161 actgtcttca acacgactgt tttgcctccg gcatttgctc cggtgataat caagcctcga
    17221 tagtctttac cgatttcaac atccaatggt accgcatgct caggtaaaaa tggatgtctg
    17281 ccttgcttga taatgatatg ttccgattta ttgattctag gtgtgcttcc attgatttct
    17341 cggctaaatt ttgcccgagc aaagatgata tctaacaccg tgatcgtttc gatcaataaa
    17401 tcgatcactt cttcgttttc tgcgatagca ccagtcaatg ccgccaggac ttgatattct
    17461 tcactgattt cttcagcttt taacaactga tagtgttcat tcaatttaga aatcacagtt
    17521 ggttcaataa aaaccgtcgt tcttttattt gattcatcga taatcgttcc atctatttta
    17581 tttttatagc tggctttgat aggaatcgta tagtattcgc ctttttgaac aatcattgct
    17641 tcttggatca tttctttgtt tttcggatga cgcaagaatt tcagtaattt tgactggatt
    17701 tctttttcga ttagttgtaa ttgtttgcgt actttacgca aattacgtga cgcgtcgtca
    17761 ctgactttat tattttttat tttctggtca atcaactctt ctacatttat caaatcagga
    17821 agatgtttgc tataagcaaa caacaagggt gtctggtatt gattcttatc aaaaaacttt
    17881 gtgatcatcc gactgcttct aagaaaatcc gcatattcga tcaaatcggt tggctgcaga
    17941 atcaaccctt ttttgacttg ttctgtcagt gcatcgatcc tcggcaaccc cataaatgga
    18001 acatgctgac tgctttctaa gatcaagcgt gcttctcttg tttcttcttg acgatccaag
    18061 acagtttgta ggtttgtcga gaccgtcata tcgctgattc gttttttgct atagtttcca
    18121 attgctcttg cttggatttc ctttttgatc cgatcaaatt gtaattgact aatcatttcg
    18181 ttgtccatat tctctccttt tcttgaagag atacctgtcc caaaataaat aagggcctag
    18241 agataaataa aaggatagaa cacagcttaa aaaagtcatg tcctatcctt attagaatac
    18301 aaagatattg ttttcctgac aggtaccgtt tcataagcac accaaataaa ctttgtttat
    18361 tggctattcg acttactgat tagataccag ctacactcac aaaacatacc ccattcttct
    18421 aggtcttatt tattttccac ttaactatag caagacacat tttggttgtc aaactttagt
    18481 atgttttata gatttacacc attaatgaat agacacctac cgcgataatt gctccgatca
    18541 ttggacctgc gattggtacc catgaatatg cccaatctga agttccttta ttagctattg
    18601 gcaataccgc atgtgctaat cgaggaccaa agtcccgagc agggttgatt gcatatcctg
    18661 ttggccctcc taatgaaaga ccgatggcta agatcaatcc acctacagcg aatacgttcg
    18721 ttccaggtgc aaattcgttt tgtccaaaag ctagcaaccc aagaactaat acaaatgtac
    18781 caattaattc tgtgatcaca tttgctggat aattccgtat agcaggacct gttgcaaacg
    18841 ttcctaaaat cgcgctttca tctttcgttg cattccaatg tggcaaataa gaaagccaaa
    18901 caagaatggc ccctgcaaaa gctcctaaaa cttgcgcaat gatgaaagga acgacaagat
    18961 tccaactaat tgcccctgtc atcgccatag ctagagagac tgcaggattc aaatgagctg
    19021 ggcccatgaa tccagaaata tagacagcca ttgttacagc gagtccccaa ccaaatgcga
    19081 tgacaatcca gccagaagcc tgtgctttac ttttgtttaa attcacagcc gcacacacac
    19141 catctccaag caaaactagg atcagtgtcc caataaactc ccccatgatt tgagtcatat
    19201 cactgttcat ttatttctcc ccctttagtt cccgcagatc tgattctgca atcaatgctt
    19261 ctaaacgttt agtctgttgc gccttctcct cttctgtcca ttcaaaatat ccagcaattg
    19321 catcgatcac aggttgcttg atctcatcaa gctgatctcg ttcaaacaac agatgatttg
    19381 tacgacgaat gagataatcg cctggggcca aaaccatttc ttcttctaaa ccataacgta
    19441 accgagctga ctcagcgaga ctcaaaccag gataaggtgc catttctttt gctaattcaa
    19501 agatacgtcg ggcattagtc ccgtaaaaat cagcgatata agtagcatct tcttcctcta
    19561 aaccggctgc tactccttct tttgctaatt ctgtcactgt ttcttctaat ttagttggat
    19621 cgaagtttcc acctgaaatc tgatattttt tagagtcgat ttctttcgtc tctattccgt
    19681 attcttcttt taataattgg cgtatcaatc gcaaagctcc ttctgccatc ttacggtaat
    19741 cggtgatttt cccaccagat aaagtcagca aaccatctgg ttctctttct aaagaactac
    19801 ctctggaaat cgtagaaggt gctttttcat cacggctgtt ttccaaatgg ttcaacacgt
    19861 cttctacttc agcacgagaa actttgtttt ccttatattc acttactgta tcgacaactt
    19921 tattgaagct cttgtctgaa atcgatccat tatctccacc attgtaatca gaaccagaat
    19981 taccaatcaa cagtggacga agtcctgccc agcttgcttc gatatcattc aatgtgatat
    20041 ttgcttctgg atagcgatga ttgatcacat ccaatagata atctacatct tcttgtgtga
    20101 ctttcggatc cgtaaaatct ccttggtaat ccgtatctgt cgtaccaaag taagtcttgt
    20161 tttctcttgg aatagcaaaa accatccgcc catcttgctt tcctgtatcg aagtatgtgg
    20221 gttgcggtac aggcagtttt ttcgcatcta caactaaatg gatcccttta gttggacgca
    20281 ttttaggaga gactggacgc gtaaaattca agtttcgtac tttatctacc caaggaccac
    20341 tcgtattgat cactaatttt gctttgattt cgatcacttc atctgtcagc aggtcacgag
    20401 ctttgacgcc aacgatttga tcgccatcat ataaaaagcc aaccgctttc attttactta
    20461 ctagataagc cccatcttcg gcagcctttt tgatattatc aatcactaaa cgggcatcat
    20521 tgttgcggaa atctagatag acacccgcgc cttttagccc ttcttttttc aaaaacggtt
    20581 ctctttccaa tacttcttct ggtgtcagcg tataattctc atatttggtt cctgtcacat
    20641 ttgccagttt gtcataaagg tccattgcta ctttgaccga gaacatattg aaggttgttg
    20701 ctccttcgcc ctcatagatt ggcaaaagca ttggatctgg ttttgggatg tgtggggcaa
    20761 tactttgtac gactgcacgt tcaccaactg tgtcagctac tacttccaca tcaaatgtct
    20821 tcagataacg aataccgcca tgaacaagtt tggtcgagcg agaggaagtc ccttctgcaa
    20881 aatcctgcat ttcgatcaat cctgttttga ttcctgatgc tgctgactgg actgccacac
    20941 cggcaccagt gattccgcca ccgatgatca acagatccaa ttcttcttgc tgcatttttt
    21001 gaatgctatc ttgtcttgtc ttgtttgaaa acatatggat ttcctcctta ttcaaatgct
    21061 tgggtagctt ttactgcttt tttccaaccg ttgtaaagtt tttctcggcg ctcttcctcc
    21121 atttgaactt caaatagctt gccagcttca taaaattcgc ggatctcgtc tgtatctttc
    21181 caataaccga cagctaatcc tgctaaaaat gctgccccta gcgccgttgt ttccaagttt
    21241 tctgctcgtt ggatcggtac attcaagata tctgtttgga attgcatcag atattcattg
    21301 tttgctgcac cgccatctac tttcaataca ggaatcttga ttccagtgtc atcttgcatc
    21361 gtatcgatga tatctctcac ttgataagca atcgactgta aagtagcttt aatgatatct
    21421 tcttttgtcg taccacgtgt caagccaaac atagcccctc gtgctgcctg atcccaataa
    21481 ggagcaccta gcccaacgaa tgcaggaaca acataaacct catcttcgct tgtagagcgt
    21541 ttcgctgcgt cttctgaatc gctagctttt tgcaacattt gaagtccatc tctcagccat
    21601 tgaatcgaag aaccagccac aaaaatactt ccttctaaag catagtaaac ctttccattt
    21661 atgccgtaac caattgtcgt caataaatta tttttggata attgcggttt ttcaccggta
    21721 ttcatgacaa taaatgaacc tgtaccataa gtattcttga ccatgcctgg ttcgaatgcc
    21781 atttgtccga ataatgcggc ttgctggtct cctgccatcc ctgaaattgg tgcttcgctt
    21841 ccataaaaat gataaccttg cgtataacca taaacttctg agttagaagt gacttttggt
    21901 aacatcactc gaggaatatt tagtaaatct aaaatctcct gatcccaatc tagctgatgg
    21961 atattgaaca acatcgtccg actggcattc gagtaatcag taacatgtgc ttgtccatcg
    22021 gtcagtttcc atactaacca agaatcgatc gttccaaaca tcaattcgcc tttttcagct
    22081 cgttcttgcg cgccttctac atgatcaaga atccagcgta tcttcgttgc tgagaaatag
    22141 gcatcgatca ctaaccctgt tttttcgtgg aaaaattctt gatagccttc attttttaag
    22201 ttatctgcaa ttccagctga ctgtctggat tgccaaacaa ctgcatgata gattggacgt
    22261 cctgtatctt tttcccatat tactgttgtt tcacgctggt tcgttatgcc aatcccagca
    22321 atctgatttg gtttgatccc tgattcgatg aacgctccgg caataactga ctgtacagaa
    22381 ttccagattt catttgcatc atgttccacc caactttctt gcgggaaata ttgcgtgaac
    22441 tctttttgag aactgccgat gtgtcttcct ttcttgtcaa agataattgc tcgtgagcta
    22501 gttgtacctt ggtcaatcgc catgatatac gttgattctg tcattgtgat tccctccatt
    22561 atatgcaaac gctttcttta actactctta tcatactact gttttaaaaa aagaaactgt
    22621 caaaatttta ttcttttttt ctttttttga cagtttttta ttatgaagct attttttaat
    22681 caattctttt cttttttcag cgtctctatt cttcttttca atcgaataat gtcgtaaggt
    22741 gacgcaaatt ctgacagata atattcacac tgttttttgt ttctctggca aaatgtgatc
    22801 accaaatcat attctttacc ttcttcataa cttccaatct caataccgcc gattcccctc
    22861 agttctctga tcagatattg ttgaaaagca atacgataaa tcggcagact ttctaaatcg
    22921 attccaatca ctactggttt tgcaatataa aaatcgatca tcaaaagaac attggcatac
    22981 tggatcatca ggtaactaag ctcactatct tcagctcttt tcctcggcaa ttgctgcacc
    23041 gctgtttcct gtaatttcgc cagtaactgt gttaaggaat gcccgagcat tttctgctgc
    23101 tgttccaaaa gccgatcacg ctcatagact tcgatctttc caacaaagaa aaaccattca
    23161 ttgttgatct gggaaatttg gtagcctact gccttttctt ctttgatttt caaacgacga
    23221 ggacgataat gccataacat cgtttcccga atgtaagtat ctaaaacagc ggttggtaac
    23281 tttttgctcc gtgtcaaatc atactgataa aatatttctt cgtctactat cgaaaaagaa
    23341 acaaaaaagc tgtaaaacat cattgtttcg tatacattca gttctgctgc tgttcgacta
    23401 agatgcatca cgatgattgg atcgattgct tgataaagcc ggtccgactg atagagtaat
    23461 ttcttttctt tcaaagttgc gtacgtactt ttttcattca atagtctttt tttcgtaatc
    23521 ccgagccagc aagcaatctg tgcttctgct gatgccgtaa acgttgtttc taatacacgg
    23581 tccaatccta gaatgaacgg tcttttttct ggtgtatttt gcaaaaaaag cggtcgctga
    23641 tcttccggga tatattggaa tagttcataa taaaaatatc gtatctgtag ctcttctccg
    23701 tataattgac cgtttttgat ttgtaatcca aattcctcta agagctggtt caattctttg
    23761 atctttcgaa aaacagaaga ctcactgata ttaaacgctg tcgctaatcg aacgatggat
    23821 acttgctgat gttctaataa atagacgagc atcttaaact tcaagctttc ctgcaataaa
    23881 aacaatagaa ttttttccaa acttacatct tcgtccaaca caagcagcgc ctgatactca
    23941 tttcgctgta cttctacttt tttttccatc attcggccaa tcaattcaat atcttccaaa
    24001 tattgatcaa acgaggcttt tgacagttgg acatgctctc tcaaaccatt gatagaggct
    24061 gttccccctt gcagaacaag gtttcgcaaa atcaggatct gtaactgctc ttttctttct
    24121 aataatgctt caatcttcat aggcagtatt cgccaccttt gaccacacca tatctggcac
    24181 acggtattct ggctggaaaa atagctcatg ttcttctaac tgttcaaacg tccatccttc
    24241 ttctactgca attgtcagtt ggtcaagaag gtggaacatc tcttttttgc tttggagctg
    24301 tgctccaaga atcttttttt cagaatcaaa tagaaatttg agtttaactt tgctttgatt
    24361 tattgctgta agcttgtagt ctacgtatcc tgtttcaatt ttttggggat aaaaaatgct
    24421 ctcttcttct gtcaaaccta cgcttcctaa gaaatagcca aaccactgat tgccgactgg
    24481 acgataggtt ccctgatcct ttgtctgtga gccactaatt gtcttgctga catttcgtgc
    24541 tgtacggaca gcgttattta ctagagaaac atacattttt tcatttgttg gacgaaaagt
    24601 tacttggatc gcatcgccaa tcgcataaac gtctttttga gacgtttgca agtagtcatc
    24661 tacccagatc gttccatcat cattcaatgt taattgttct tcccacatct gattgtcggg
    24721 acgagaatga ttagccagca aaacaagatc atagttctcc tttctttttt ccgtctctac
    24781 aatagcggtt tcattttcgg tcaaagacac aacttgttct tctaaaaaaa gtgaaagccc
    24841 ttgatttttc atttccttta ctaacggttc tgtcatttca ggatctagat aacgaaaaag
    24901 aattgacttt cggctttcgt aaagatggat ttgttttttt cttgatgcca acccttcagc
    24961 tatttcgatt cctgcttgtc ctgcaccaat aattgcaatt ttctttgcat tcatgatctt
    25021 ttgctgaaga gccgttacgt tttgtatctt tttaactggg tgaataagcg ttgattctgc
    25081 agaaatattt cgaaactttt gatttgaacc agtcgctaga atcaaacgat caaaatccaa
    25141 tttttttcct cctgctaaaa cgactttttt ttctttcaat cctattactg tcttttctgt
    25201 gtaaacatgg attcgatagc ggtcagtcag ttcttctttc gtgatccaag aaagttgttt
    25261 ttcactggta acctttcctt ttaatatcaa attgaacccg ctgggaatcc atcctaactt
    25321 cgattgcttt tcaatcaaat atatttcact tgttggcatc aattggcgaa gataaatagc
    25381 cgcatgaatc cctgcaaacg acccgccgac aattacaatg ttcatctgca ttcctctttc
    25441 ttataaggtg ttctactgct aacttctcaa tcagaaaaca cagtattcat aactcgcttt
    25501 caaccttatg atacatagcg atgcagcaga atcaaccctc tccatcatac cgtgttctag
    25561 gtctgcttct caataatgta ctagtggacc taattgaaga gtatcatttc tgaagagatg
    25621 tttcaaaaaa tgtactctta taaaagaaaa aaactcgcct tttacgtacc tctgtccaaa
    25681 taggaaacag aaaagaacga aaagacgagt tttagcgtgt gtaatgtaat agttagaata
    25741 gacacatcat ttatttttaa ttctttaatg cttcaacaac tgcttcgcct aattgatgag
    25801 cagattgagg attttgccct gtaagaagcc gcccatcaat ttgaaccact tctgaaaaag
    25861 ctgctccaga ctgatagttt gctccttttt ctttcaaagc atcttctgct aagaatggta
    25921 cttcattcgt tgtaccattt gcttcctctt cttcattact gaacccagtc actgtacgtc
    25981 cagctaaaat agattttcca gactcatctg taactggcaa caatcctaca acaccgtgac
    26041 aaacagctga gaccagccct tcatttcgat agatttgtga agcgactttc ccaagatcta
    26101 ttgcttctgg aaaatcccac attgttccgt ggccaccagc ataataaacc gctgcataat
    26161 catttgcatc cacatcatca ggacgtaagg tatttcccaa ggcccgattt ctgaaatctt
    26221 catcttcata ataggcccaa tcgatttcat ccatattcaa aacactttgc ggttctaaag
    26281 gtacatatcc gcctttagga ctgacaaaat ctgcatcgat cccatttttt actaatacat
    26341 cataaaaatg agtcagttca cttaaccaca aacctgtagg acgctcgatc ttttcgtact
    26401 tttctacatt tgttaatacg atcaatactt tcattttccc atcctctttc tataaaaatt
    26461 gcgttattga taacgtgctt tcaagaaatt atataatggg cttggcaaat aaagctcttt
    26521 atatgcttct tcaaaaggtt tcgttacaaa ttcatcctca cggttttcca ataatttctc
    26581 tgcccagtca ggatcgacga tcatttgctg tccaattgct gcaagttctg catatttcaa
    26641 tacctgctct acatcttcgc gattgcggat cccaccgaca ccaactaaag ggatctttcc
    26701 ttgaatcgtg ttatggacca cttcaagaat cgatttatcg ttgtatttat cagaacgagc
    26761 tattcgatca tacatattta atgaaacatg cagataatct agttttgact cacgtaatcg
    26821 ttcaagaagc cagatcgtgt cttcaaaacg gatacctggt gtttcatatt cttctggtga
    26881 gaaacgatac cctacgatga atggtttttt agcatattgt tcaaccgctg agaaaaccgt
    26941 tgttaataat tcttcaataa aacgataacg attttccaag ctaccgcccc attgatcctc
    27001 tcgtctattc gaatgcggag agaaaaattg ttggatcaaa tacgtatttg ctccatgcaa
    27061 ctcgattcca tcaaatcctg attcaatcgc tctacgtgtg gctttgccaa atgcttggat
    27121 tgtttcaacg atttcttctt cactcattgc tcgtggtgtt tccgcatcag gacgttttgc
    27181 tgcaactgca cttgcagata caggctgttc tccagataaa gttgctcgac ttgtcattcg
    27241 tccaccgtgg aaaatctgaa taatcgcttt tgcgccttct ccttggattg ctttcgctag
    27301 ctcacttaat cgaggaatca tttcatcatg agcgatgctg agttcacctg gccagccttt
    27361 tccgatatct tggacatttg cagcaccagt aataacagca ccaagacctt tagaacgttg
    27421 tttataataa gtgatctcat cttcggtgat cactccattg aaaaagctga gttgtgtcgt
    27481 cataggcgac agcatcaatc ggttcttcaa ttgaactcct gaaggtaaag taattgattg
    27541 atcaaattgt gacattgtca ttcctcccat aattcgctct gattaaccta tcgagaatca
    27601 tctatcatta aaccactcga ttagttgtac atgcaactaa tttgcttaaa aaattttatt
    27661 ttttttgtaa cttatctgtt gcatcattca ttgtctgggc taattcttta cttgcttcat
    27721 ttcccaaaac tgctttgtat gactgatgaa gttcttccca gctaccatcg acttccttag
    27781 caaattggcg tccttgttct gtaagataaa ctaatgacaa gcgaccttcg ctaactgttt
    27841 tgacgtatcc tttatattct aatttttcaa caaaacgagc aatcgttgat ggggcggaat
    27901 agagcatttg tgcaatatct ttttgtggta ttccatcttt ttcttttaat atcaataaaa
    27961 taaaaccata agttggtgcc agacctgtcc ttctaaacgc ttcatcagct aatttattca
    28021 acatgcgatc tagctttttc acgctaaaga aaagacattc atctaacgct ttttcaattg
    28081 gcttttccat ttcatcccct ccattggcgt acatatcata gtatgatttt tattttttgt
    28141 caaaaaacac ggaataatgt ccgcctcctc tgtctagatt tgaaaaaaag aagtagccat
    28201 cttcaaagaa tttcggcttg ttttcgacaa aactccttct ggaacaccgt gaatcagagg
    28261 ttgtgaaaaa gtcgtttagt tttgagtatg aagaaaaatt ttgtgaaaat ggctttccac
    28321 atttttgcat aaatttggct taataccgaa aaactggctt ttgaacaccg tttatatcag
    28381 aggttgtgaa aaagccgtcc tgcatcaagt aataagaaca gccaaacaaa aatagttcct
    28441 catattttgt cgttttttga gctaatgccg atggtcagct gattgtacac cgtttattga
    28501 gaggtcgtga gcctgccgtt gaactccgag tcacaaggag caatttcaaa aaacagcttc
    28561 tcatgtttta tgactatggt gacttgtgat cgaggagttg gcaggagaat actgtttatt
    28621 cgtaaagaag gggctgtgac aagacttttg tcacagtccc ttatttcctt ttattatagg
    28681 atttcgccat ttgattggat gacttgtctg taccaatcaa atgatttttt ctttaaccgt
    28741 ttctttgtgc cattcccttc gtcatcttga tcaacataga tatatccata acgttttgac
    28801 atttgtgaag tagaagcgct gacgatatca atgcagcccc acgacgtata accaaataaa
    28861 tctactccat ctttgatagc ttccttcatc tgctcaatat gctgcttcaa atagtcaatg
    28921 cggtaatcgt cttgaacggt tccatcttct tctactttgt ctatagctcc gagtccgttt
    28981 tcagctatga acagtggttt ttgataccta tcatataatt gattcaaagc aattctcaaa
    29041 cccttaggat caatctgcca gccccattca cttgatggaa gatacggatt tttaattcct
    29101 ccagttatta agtttccttg gaccccttcc caattttctg gatgagcact gactgcacta
    29161 gacatgtagt agctaaaaga aacaaaatca acagggtagg ctgctaagat ttcttcatca
    29221 cctacttcta tttttaagtt gacacctttt tcttcaaaca ttcgttgcgt atacgctgga
    29281 taatatcctc ttgcttgaac atcactaaag aacaaagaag ttctttgctg ctcaattgtt
    29341 ttttgaatat catccggatc acaagaatac ggaacaagaa gctgatagct gatcatacaa
    29401 ccgacttgtg aacctttgat aatttcatga caagcttttg tagctaaagc acttgctata
    29461 aactgatggt gtgcaccttg gtatttcgct tgttccaaat ggggatggtt ttcttcaatt
    29521 actcctacgc taacaaaagg gtgatgtttt acacagttga tctcattgaa agtcatccaa
    29581 tattttactt tatttttata gcgtttgaat actgtttcag cgaaacgaac gtaaaactga
    29641 ataagctttc gattaggcca tccaccatat tctgtaacca aatataacgg catttcataa
    29701 tgggaaagag aaacgactgg ctcaatccca tattttagac attcgtcaaa cacttgatca
    29761 taaaaaaata atcccgcttc attgggtgtt tcttcatcac catgaggaaa aattcttgtc
    29821 catgcgatag acatccgata gcatttaaaa cccatttcag caaataaacg gatgtcttct
    29881 ttaaagtgat gatagaaatc atttccgtgt cttttaggat aataatactt gctgttggga
    29941 tcctgcgccg cttttacttg ctgatgcgtc atcatatgcc attgatctgt ccatttttct
    30001 ttaggtaaat cgctgtcgaa tgtatacaca tcagacacag ataatccttt ttcatctaca
    30061 tcccatgcac cttcacattg attggccgct gtcgctccgc cccataaaaa tccttctgga
    30121 aattttttca tatcgatccc cctaaacttg ctagttttct tgtttgatta tttttcttat
    30181 gactagtaat gatatttttg aatctactac tatttgtaac aataaccatt gtggtcggat
    30241 catatccctc tttgattaat tcttgcaaat ctacttccat caataaatcg cctttacgta
    30301 ctttttttcc atcagatact gcactttgaa agtatttgcc atttaaattc actgtatcaa
    30361 taccgatatg gatcagtatc tctgctcctt cttcactgac taccccaatt gcatgtttcg
    30421 ttggaaaaac tgttgaaact tccccgttta cagaactgta gatttttccg tccgttggaa
    30481 tgatcgcaaa cccttctcct aaagatccac tagcaaatgt tggatcgttt acttcagata
    30541 aatcaattat ttcacctgct gcaggagtac ttaacactat atcctttgag gattcttctt
    30601 cgatttcatc cttgtagccg aaaagaaaag ttagcataca ggaactgatc atcgctaaga
    30661 aataactagc aactagccaa acaaatcctt caccaatata aatcgggaaa gacaaaatac
    30721 ttcttgtagc aactgccaaa gcaccggctc ctgcggcacc agctactcca ccaaatatag
    30781 ctccacatat acaagcaatg taaaatggtc gtttatattt aagagtcaca ccataaacag
    30841 caggttcagt gattccaaat aatgctgtga taaatgcaga taaagataaa ctttttactt
    30901 tcttattttt cgttttcaaa aataccccaa gtactgctcc tgcttgcgca gctacggcag
    30961 tcatacatgc tggacctagc gtatcacgac cgtagaaaga aagattgttg gtcattactg
    31021 gtacaagtcc ccaatgtaac ccaaaaatca ctaacacttg ccaaaatccg ccgataacag
    31081 caccagataa aacagggcta aagttataaa cagctaaata tccagctgca atcagctgac
    31141 ttgcataatc tgcaattgga cctacaacta ataaaataat tgggaccatc acgatgatac
    31201 aaatgaatgg tgtcaataaa tttcgaatac tttcgtgcaa gcttttgcga atcttaggct
    31261 caatataaga aagaacccat acagctaaaa ttgctggaat aaccgtactt gtataccttg
    31321 ctagaatcac gggcatccct agaaatcgca aggttatgct atcactatat gcactgacaa
    31381 tcgtaggata aacgagagct gctgcaataa ccatagcgat gaaacgatct gtattgaact
    31441 tttttgcagc agtatatgcc aaaaatatag gcaagaaata gaaaaatgca tcagctgctg
    31501 cattcaaaat gatataagtt cctgattcag tagttaataa attcaaactc gtacataagg
    31561 cgagtactcc tttgatcaaa ccacttcctg ccaagacgcc taaagtcggg gcaaaaacag
    31621 cggcgatcat gtctataaaa gagtttaaaa tgcctttttt ctcagttcct ctctttccac
    31681 tagatgcatc atcttctagt ttcatttgtt tggataatac gtcatacact tcatttactg
    31741 catttcctac tacgacttga aacattcctc cagcttcgac tacagtgatc acaccttcta
    31801 gttgctctac cttctcctta tctgccttct tattatcttt tagggtaaag cgtaaccttg
    31861 ttgcacaatg gtaaacattg ctgacatttt cttcgcctcc gacaaatcgg acaatatctt
    31921 tcgctaattg tgaatagtcc atttttactt cccccttgtt aattaatgcc gtagcaatcc
    31981 ttcttacaag ataagtatag ttcttagaaa acggtttctc cattcaatta gtaccccttc
    32041 taatagcgaa cgaaactgta caatagtgtg tatggtttac ttagccgagc aaatcactca
    32101 ctgcttgaaa gaaaacaagt atatcgtttc tcttatagtc aaaggagaca aaaaaaacac
    32161 gcttgatctt cctttgctga tgtataagtg gtatctcaag ctgatactct gtgatttttg
    32221 tttctacttg gcttttgata agacagcaca acaaagtctc ggcatttaac ggaagcataa
    32281 ctaggtcaga actaggagag attagttgct gaatggaatc tatcttttta tggattttta
    32341 tttgattttc ttctttgtag taatgatcaa gcagttttgt tttctttttt tcttcaacta
    32401 tggtcagctt ttcacgcaaa atctgataaa tctcccaaga ttcaaactca tctagaacac
    32461 taggtagata aatcaaatca tcccactcaa aaataagctc ttgctcagta gtcaaaattg
    32521 catcatattc cgttattttg tttgattgat ttttgaaaag atacactggt tctatccggc
    32581 attctttgat ccacatgttc atactttgtt gaatctgttt ttttagtgta ttcaaaatac
    32641 taattggctg gttactaaca atcagtatct ccccttgttt tcgaaaagga ttcaacgctt
    32701 cggcaagata caaaacaaaa taagcgcttt cagctaaatt gggacgtaat tgaaaatgta
    32761 ttgggaagaa acgccgaatt aaataaattt ctaaaggata acgggagatg atcttttgta
    32821 caaaatgatt catggtatta tgtccggcat ccatcctcgt tttcatttgt ttgatatggg
    32881 ttagcaaaaa ttctgtttca tggaacaata acgaaacagg aagatctaaa aaacgaataa
    32941 tcgtttgctc aaatgctctt agattcttct ctatttcttt gtttttttca tcattaatca
    33001 tcaaataatt taactctaat agtcgatttt caattgcttg tttttcttga ttggaaaaag
    33061 aatagggtag ttctgatgat aacttttttg tgagggtctc taccatcgga ataaataatg
    33121 tatacctgtt tgttttttct aaaagttctt ctcttcgaag aatggtcaaa acgagatacc
    33181 gagaaaatcg tatgaagtcc tctcctgaaa caatataatt atatgatata agaacctctt
    33241 ttaatgtctt ttggacagtc ggcatgatat tggaaaaaat tgtactgatt tgttgaggaa
    33301 accttgcgtg ttggatcacc gtttctgttc caacaagaga taagaaaatt ctttttgaat
    33361 tttcttcagc aatgattttt agtccttttg tcgacgaaac ttccagttct aatttaggat
    33421 tgcgctcgat ccaacgcaaa atcgttttta tttgtaatga tacggctgtt tttgaaagaa
    33481 agaaaatgtc tgctatcttt tgcatactga catacccttt ttgaaacaat aagtacgtca
    33541 caatagttga agctcggaaa tccatgctgg aatagatttt atgtttcgtc acctcttgta
    33601 tgatttgctg tttagtttca gattcaattg attctaaaaa ataacctgaa cgtttatttg
    33661 actggataat gattttcttc tcgtagatag caagcatatt tttgatttca gtttggatcg
    33721 ttttcttgtt ggtacctaaa gcttcagcta attcttcgct tgttatccaa tgattcttat
    33781 ttcttaatac tacaagaatt tggagttgtc gtttatttaa gtccatttgc ttacacctcc
    33841 tctttgttat aaacatacag gaaaaacctc tcaaatgaca atagtcactt gcagaggttt
    33901 aaaaatcatt tgaagaaaga tctatattca aggtttttac cattagactg gataatttgt
    33961 ttataatatt caaaactatc ttttttcaat ctcctaaagc ttccgctccc atcattttgt
    34021 ctatccacat aaataaagcc atatcgcttc ctcatttccc ctgttcctgc cgatacgata
    34081 tcgattggtc cccaccataa gtaaccaatg atttggcaac catcttttat tgcctcagca
    34141 atttcctgaa gatgtatttt tagatactca caacgttttg gatcgtggat tcgattcaat
    34201 tcatctggtg cttcatctag tccaatgcca ttttcaacta tgaataatgg taattcataa
    34261 cggtctgtca attcattcat gacataacga aaccctagag gatcaattgg ccaactccaa
    34321 ggttcgggag atttttcctt caaaaaggga ttatcgaaac catttgctcc gccagtatct
    34381 acgcgcattt ctgcttcttt tttatagaca gcgctacgat aataactaaa tgcaatataa
    34441 tctgccgtgt tagcttgcaa gcactgttcg tctccatcta acattactgg tgcacagtct
    34501 tcttccttcc atgtcctgta aatataagct ggataatgcc ctcggcacat gacatcggag
    34561 aaaaaccatg aattgcgttt aaattgcagt gaaccaaata cattctctgg atcacagtca
    34621 tatgcataag tcgctaatgc agaaaagcta cacataatac ccatcttagc atttggatcg
    34681 attttcttta ataattgagt agcacgggcg ttcgctagaa acatatgatg acaggcttga
    34741 taaatcactt tttttgttag tgtagcaatg ggttcaagtt tatctctagc agggaatgta
    34801 accaatccag cagctgcata aggcatacga aaagcattat tgatctcatt aaaggtcatc
    34861 caatatttca ctttcccttg gtagcgttca aagacggtct gcgcataaaa ttcaaaaaag
    34921 tcgattaatt ttcgattttg ccacccccca tactctccta ccaatgctaa gggggtttca
    34981 taatgactaa gagtaattac tggctcgata tcatatcgat ttaatgtatt gattagtctg
    35041 tcataaaata cgagaccagc ctcatttggc ttctgttcgt ctcctcttgg aaatattcgt
    35101 gcccatgaga tgcttgttcg aaaagctttt aaacctaact ccttcagtaa acgaatatcc
    35161 tcttcaaatc gatgataaaa atcaatcgct tcatgagaaa gataatggag tgattcgtct
    35221 aaagcaatct cccaaatctt tttttcttca ttccactgaa tactaggatg ctggttcatg
    35281 atcccgataa gcgtatctgt cacattaggt aatttgccgt cttccaacca tgcaccttca
    35341 cattgattag cagctactgc tccgccccat aaaaaatttt ctggaaatat ttcctgatcc
    35401 ataactatct ctcctcgcta tcaacaattt cttacttgaa gtatagctcg attcatcaaa
    35461 acaagatatt ctaactattt cggcagaaga gaagaaaaaa attgaatgct actcttcatt
    35521 tttaatattt atatataaca aatacataag acgatccccg aaagaaaaca ctcgttaacc
    35581 atctaagcaa acgatttatt ttacttccaa cacaaatgct tcataaggtc gtaaatcaac
    35641 tttgccgtct ggtaactgtt tcagaggata atttgacaca ataaccctta aagcttcacg
    35701 tggaatagtg aacgtctgct tttcactgga catattacaa ataacgagcc attgctggtc
    35761 ttcatatatg cgagaatagg caaagacatg ctcaggcgtc ttttccacta attcatagtc
    35821 tccccagacg atgatgtcat gttttctacg caaatcgatc aatttcttgt acatataaaa
    35881 aacagaatct tgatctgcta acgcttcttc tgcattgatt tctttataat ttggattgac
    35941 tcgaagccat ggaattccag tagtaaaccc agcatgttca cgggcgttcc attgcatagg
    36001 cgtcctagca ttgtctcttc ctttcgcctt gattgattcc aagatttcct cttttgtaaa
    36061 tccagaagaa atcctttcgt tatacatatt gattgtctca atatcttttg cttccgcgat
    36121 atcatcgatt gggcaattcg tcatgcctaa ctcttctcct tgatagatat aaggggtgcc
    36181 tttcatcata tggagcaaga tagcaaacat cttggcactt tcttttcgat acacatcgtc
    36241 atttccccaa cgagaaacaa ttcgcggaag atcgtgatta ttccaaaata aactattcca
    36301 tccttcatca cctaaagatg tttgccattt ggataatact tttttcagtt cagagatact
    36361 caagggtttc aaatcccatt tgtctttgcc ttcttgctga tctaatccta tgtgttcgaa
    36421 ttggaagacc atggataatt catttctttt aggattggaa taaagtttag cgatttcggt
    36481 agttgctccc catgtttcgc caacagtcat cagatttttg tcaccaaaag ttgcttgatt
    36541 catttcctga agatactcat gcagtttagg tccattactt gtgatttctt tatccggttc
    36601 tttgccaatc atatcgatca cgtccatacg aaagccccca atacctttat cgatccagaa
    36661 attcatcatt tgataaatct cttggcgaac ctgtttattt ttccaattca aatcaggttg
    36721 acgcttgcta tacaagtgca gaaaatattg ccccgttttt tcatccaact gccaagcaga
    36781 accactgaat attgagcgca atctattagg ctcttttcca ttgaccggat cacgccaaat
    36841 ataatagtcc cgataaggat tttctttatt tttacgggcc tcaatgaacc acggatgttc
    36901 atcagaagta tgattcacca ccagatccat gatgatacgg atatttcttt tctttgcttc
    36961 ttcaatcagt tcttccattt cttccatcgt accgaattct gccatgattg cttgataatt
    37021 actgatatca tacccattgt catcattcgg agactcgtat actggagaaa gccaaattgc
    37081 atcaatacct aaatacgcca gataatccaa cctggaaata atcccccgca aatctccaat
    37141 accatcttga tttgaatctt gaaaacttct tggatagatt tgataaacaa ctgaatggtg
    37201 ccaccatttt tgttccatga ttttcctcct ataaatggat gtttctattt tttgcttaat
    37261 catttatttt agtctctctc actaaaaatt gtaacgcttt caaatagcta ttgcaccttg
    37321 ttatcggtaa cagaataaaa caaaaaaaca gctgaggaaa ttccctcaac tgttttttga
    37381 aaggattgat ttatgcaggg aagaatgctg atttcacaac ttctttataa acggtgttca
    37441 ctcagctgtc ccttggtatt agctcaaaaa acggcaaaat ataagaaact attttttcgt
    37501 ttttttctct aatactcagg ccagaacgct gatttcacaa cctctttatt tcacattcaa
    37561 tatccacatg aatcccacaa atacaaagaa cagaccatac atgattggag atacttcttt
    37621 tcctctttta gctgctaaca tggtaattgg atagaagata aaacctaatg caatcccatc
    37681 tgaaatacta taagtcaaag gcatgccgat caagatcaag aatgaaggga tcgcaatctc
    37741 taagtttccc cattcgattt gtcgcaaaga ttgtgccatc aatactccta ccataatcaa
    37801 tgctggtgca gttacttgtg aagtaacaac tgatagtaat ggagaaaaga acaagcctaa
    37861 caagaagaaa atccctgtag taatagctgt gatacctgaa cgtcctccaa cggcgatacc
    37921 agctgatgat tctacaaatg caccaacagg agaagtacca aataaggaac ccgcaagcat
    37981 agctgttgaa tcagaagcta aagctttccc tacacgaggc atcttgttgt ctttcatgaa
    38041 acctgcttgg tttgctaatc caactaaagt tcctgctgta tcaaagaaag tcacaagcaa
    38101 gaatgtcagc actacgaccc acatttgaag agaattgatg tcccctacat gttttaatgc
    38161 aactaaaaat gttggtttca aacttggtgc tgcggcaaca atttcagaag gcattggaat
    38221 caaacctgtg accagaccta aaatcgttgt agctgccatc ccgatgaaga tgccacctgg
    38281 tacacgccga acaagcaaga tagcagttac gactaatcca aaaatcgtca accaagtaga
    38341 accaacgttg agtgatccta atcctactaa tgtagattca ttcgaaacga tgatcccacc
    38401 ttcgctcaga ccaaggaaag cgataaatag accgataccg ccagaaatcg catatttcaa
    38461 atctgctggg atcgcatcaa tgatcatttc ccgcagcttg aagattgtga tcaaaataaa
    38521 aatcaaagaa gcaacgaata cgccggctaa tgctgtttcc catggaatcc ccattcctaa
    38581 acaaacagaa taagcaaaaa aagcattgat cccaagagca ggtgccgttc caattggata
    38641 acgggctaat acccccatta aaacacagcc gatcgcactg gctaatgcag tcgcagtaaa
    38701 cactgctcct tcatccattc cagaagcacc taatacagtt ggatttacaa ataagatata
    38761 agccattgag ataaatgttg taaatcctgc cagtacctca cgttttaccg tggtgttcaa
    38821 tttttctatt tcgaagtagg aaagtatttt ttccttcatc ttccatcccc ctaaatccga
    38881 acattataaa taaagtccgt taaaatttcg atttattaat aaaacaacgt gttttatcat
    38941 atactaaaac aaaacataag taaacaaaaa attcggattt taagcaaaat ccgaacgttc
    39001 catctgatta aatcccttta tctcaaagtt catgaacatt cactttggat tacaagaatt
    39061 tctttattgt gttaataaag tttcttcatt ctagtctcaa aaatcaatag tgtgatttga
    39121 ttttaccatt tcgtttctgt tcttctttga tgaattcttt tgtatcgaca ggattcagat
    39181 tatttagtcc gccaaaacct gtatcgacca tttttcccca gccttttgtc ttcttttttc
    39241 ctgatttgag attctttgct cgaagcttct cactatgttt tgctcgccga tacttcaaat
    39301 aaccttctaa tcttcccatt agatccactg tttttcctcc tttttattca atcagacgac
    39361 tcagcttttc ctataacaaa ataattgcct tcagggtcag aaaaattgaa agtcaagatg
    39421 ccattgttat ctgtgatttc tcctggttct tccatgttct cgtagatagt ttcaaattca
    39481 tccgtaaaaa atactaaaga aggcggtggt cctaatacct ctggtgaaaa cttctgcaca
    39541 aatacttttg ggaatacgcc taattctaat tcttttgaga tgctgagtac aatactctta
    39601 aattcttctg gcaattcgat tgtttcagaa atttctgcac caattttttc tacaaagaat
    39661 cggcaaatca tctctacgtc attgacataa agcatcacac gtgttttttg tatcatcatt
    39721 tgacccttct ttctatttga ttttctttcc tgtctataaa ctgattccat tatatcacga
    39781 aagcgcaaac accttgtgtt tgatactatt caagaaaaat aaagcaatag tttctaggat
    39841 tgctttcctc cacaaacttt ttttacaaaa aaaaaacggc aatgaatgcc gctcttctta
    39901 tttcgattct tttataattc tgcttctgat tcatctttgg ttagtgtgtg tctaacacta
    39961 gttacgccaa atgtttgatc gattttttcc ttcaacgctt cgattgcctg tgcgccacgt
    40021 gccgcttgtt cagcattgat ggattcaatg accagcaccg ctgcttgtgt ttcttccgtc
    40081 agcatcgttc tttgggcttc acgccagttg agacagcgac agatcgctcc ttgttcatca
    40141 tagtagatga tttcttccgg aagtgccgga gcatcggaat ctgcacctaa aggcaggaat
    40201 gattcgccac cttgggcttt tcctaaatgc agatttcctg cgatcttttc gatatcttct
    40261 ccgccgcaag gtacgccaaa agacaaggaa atactattat agatatctac aagcggattg
    40321 attgggaaaa attcccgttc ttgatttgca cgtttcagta aagcttcaat agaagaacgt
    40381 gcaccttttt tagtcttgaa tgtggtaaat gcctgacgcc actgagcaat gacttcgttt
    40441 tggctgaatg tttcttctgt aaggtaattt cttgcttctt ttgctgcctc attcaataat
    40501 tgatgatacg tttcttcact ttcttttgtt acgtggttat cgataccatt tacggataag
    40561 atttcgatgg ttgcttcagg aaatacttcc cagaaattgg tatccactat tacttttttc
    40621 atctgttcac acctcttgat tagaataatt gcttgatgaa tagatagtct gtttggggat
    40681 catctcccat ataaaaaaca tgttcgctgt atttgataaa tcctttttta cgataaaaag
    40741 cgagcgcaac atcgttgtgt tcccaaacac cgagccaaat ggctgttttt tgcttttctt
    40801 ttgctttttc aatagctgta tctagtaaaa atgtaccgat accttgatgt ttaaattttt
    40861 ttcgtacata gattcgctcg atttctaaaa aatgatcagc catttcctca gattgactcg
    40921 ttcctgtatt tagtttcagg taacccgcag gctcttgatc gatactcact aggaaaaaat
    40981 cggtttcttt atcttttagc tcttctgtaa gtttttctag tgcataagct tgtgtcaagt
    41041 aatcagctaa atgctccggt gaattatctt ttccaaatgt atctgtaaat gtttcgatac
    41101 taatttgttg tagtaagtct acatctgaag gcgtcatttt tcgaatttcg tatttattca
    41161 cggcttcttc ctttcaatag atccttttat tgcctttttt gacaaaattc caatcttcgc
    41221 taacattctt gcatacttta tttaaaagat attccaattg ttttgcttct tcgtcagaaa
    41281 gaccatttaa tgcgactgca ttggagtagt tgttttctcg aatgatgaaa ggatagattt
    41341 ctgctccttt tttagtcgga tagagtttct tgattttttt attttcttta tcttccaaac
    41401 gttcgatcat cccattggat tctaatttct tgattgctcg ggcagtcgtt gtacgatcta
    41461 ctttgatcat ttctgctagt ttttcttgga tgattccagg attttcgcaa atacgtacta
    41521 aataaagata ctgtcctctt gttaaatcga cttctttgaa ttcgatattg ctaatagaat
    41581 ctagcgctcg ggcaatcatt ccgatgtctc gaaggatttc agccatttca tcccaccttt
    41641 attttgtttg taagactagt tcacctcttt ttgttgcaaa tgcaactatt ttcaataaaa
    41701 aaatcaagtt cttttttaat aactagtaaa gtatattctt ttcttctttt acgaaaaagc
    41761 tcaatcacaa tagatctggt ttttgttttc taaaataagt aactgtttct tcgttgttcg
    41821 cttttctgat agctttggca actcttctat actaaaaaat cccatgttgg aagtttcatt
    41881 attctcaata aacttggcct cttcaccgtg gatcgcacaa gcaaaaacaa gtttataata
    41941 ctgaaaaagc tgtggaatat ctttttgttt attcgtatca aaaactgctc gcaactgagt
    42001 agtttctact gttaatcctg tttcttcata gacttcttta cgcacatttt cttcaggaga
    42061 caatcctatt tcagcaaatc caccaggaag tgaccattct ttcgtccgtt tatcttcgat
    42121 tagtaggacc ttgccatctt ttttgatata ggcacgaaca tctacttttg gtgttggatg
    42181 tccttctcca atcgttgtga gcgcttttat ttcttcgaaa gtttcttttc ctatttcact
    42241 gatcagttcc aatgaaatcg tccggatctc ctcaaaacgc tctttatcaa atacatcttt
    42301 tccataatat agaccagctt gagaaagtgc gagtagtctt ttgtattgag ccaacttctt
    42361 gttcattctc ttcccttctt ttttagcaaa tgtatgacta tgtaatcaag tatagaagac
    42421 tggtttaatt acggcaaata aatataatga aatctgcaat aaataaaaga atgttaaaaa
    42481 actcgagaac aaaagacatt cgacttctct ggggctgtga ataaatggcg ttcaaaagct
    42541 tgcatctgcg gtaataagcc ccaccaaacg aaaaatatga cgagctattc ttgtctggct
    42601 gttcttatta attgaggcag gacagctttt tcacaacctc cccactaaac ggtgttctcc
    42661 taccaactcc tcggtcacaa gtcacaatat tcatcaaaca tgagaagctg tttttttgaa
    42721 attgctcctt gtgactcgga gctgaacgac aggctcacga cctctcggta aacagcgttc
    42781 aaaagccaat ttttcgatat taagccattt ctttgttact tattgtcatt gatttctttt
    42841 tctaatttat ccaatagtgg agtgtaatcc acttgagtat agtctgttac atcaaattca
    42901 tatagtgttc ctaactggaa tttgtcatat tttcggtcgg cgatgatctt tttgaatgct
    42961 tctttcgtaa tatgattggt ccctttcgtt cgatcttcca atacgtcacc atagtgataa
    43021 tgatccatga tatagctcaa atgacgatct gattcacgat cacgtttgta ccgacgttgc
    43081 cataacactt caaagtctgc tgttaggcga atcgtgatca cctgatatcc gtatctatct
    43141 gctaattctt ggagtcgtgc tttttgctta tcactaaaag gatactcgga cacaatcact
    43201 ttctttcctg cttccatata caaatcaagt gctttgtaat agaaaggata aactttcgtc
    43261 ctttcttgtt ccgcccgttc ttccaaattg ttaaacccga tggattctgc atagtattcc
    43321 ttgatttcat cgagcgtgat ggcatacata tctggaaatc gttcccgtag tttattcata
    43381 agatatgttt ttcctgtacc aggactacca gccagaagaa tgaaatactt tttcatctca
    43441 tttatttcct aagttaattg gactttccaa gttcataaac agtccaatct tatgcttgac
    43501 aatttctttt actgcttcat ttgctggaat cacattatgg cgaagagact tgttgatttc
    43561 tgtttcacta aaggtttctt tagctacttt cgtccagttg actaaaagct ccgttccaac
    43621 attgaatttg cggatcccgt tattgatcaa ttcaggataa tcttcttctt tcacacctgt
    43681 tcctccatgg ataactaggg ggacatcaac tactgcatcg atttctttca gcaagggaat
    43741 gtttacttct gtttttgatt tgaattgtcc atgattcgta ccgatagcaa tagctaatgc
    43801 atccacgcct gtagcttcta caaatttcac tgcgtcttcc ggtcttgtat ataccttgtc
    43861 attttcagcg acatggattc cttcttctgt accgccgatt gttccaagtt ctccttctac
    43921 ggaaacaccg tgcgcatggg catattctac tactgctttt gtcttcagga tattttcttt
    43981 gaaaggcaga tgggacccat catacatgac agaagtatat ccgctgtcga ttgcttcttt
    44041 gatatcatca aagtctcggg cgtggtctaa gtgcaagact gcatcaacca tttcttcttc
    44101 tgcaatagct gtcattacat tgactaacac atgcatgccg atatatttag cggtatcgac
    44161 acttgtttga ataatgatcg gcgaacccat ttcttttgct gcgcctagca tagcaggaag
    44221 catttctagg ttatgcgtat taaaagctcc aactgtaaaa ttcaactctt ctgctgtttt
    44281 tgttacttct ttcaatgttg tatacatctt ttattgccac cttatctttt tatttgtttc
    44341 ctttgatcgt tacttgtttt gcaatttgtc gcatctcatc cattttgccc atgtagtaat
    44401 cgatcccttg atcatcacca gcacgagcag catctgcacg cttctggtta tagaggctca
    44461 tcagtttttt atacccatct ccgatcgtct gtttattttc caaactcttt tctaaatatt
    44521 ggatggcttg atcgttcaca tacaattcag aaaatttaag tccaacttgt tcataaagtt
    44581 tcgctaattt atgcttatct tgggtagtgg aaatctcttg tttcaattgt gtaatctctg
    44641 attgaatctt ttcggtcgct tctggcgata gggctttttc ttcttttgcc agaacttttt
    44701 tcggttcttt ttcttttttc ttaaaaaatc caaacatcat tttccctact ttcaattaaa
    44761 atgctttcaa aactgggctt aagatccagg catataagag tgctgcggct actgtatcta
    44821 catcggttgc tgtagcattg ttgaatccca tcgaattaaa gatcgttact agcaaagcgg
    44881 gcaataatgt aataaagaaa ccatgagcaa taccgccaat gatcgctcct cggcgtccgc
    44941 caactgcgtt gccaaatatc cctgctgtcc ctccggcaaa gaaattggtt agcataccag
    45001 gtaaaatcat cgctaagccg aacatgggca aagtaaacat ggcaataatc gtaccaatcg
    45061 ttgtcgtaat gaaacctaaa atgacagcat ttgggctata aggaaagaaa actggacaat
    45121 ctaaggcagg gatcgcatca ggaactaatt tcatagctat ccctcgaaat gccggcacga
    45181 tttcacctaa cagtagacgt acaccagcta gcaatacata cacacccact acgaattgga
    45241 tggcttgtaa aaaggcaaac atgatatagt tttgatctcc tgataattca gaagcgaatg
    45301 gttcgcctgc aaataaaaca gtgatgatat atagcgggac catcacgacc atgacagaca
    45361 aataagtatc ttgcagaaat tcgaaatgtg tcggcaactt gatatcttcg atcgactttt
    45421 tattttcgcc tttctcacca aaaagcttag caacaccggc ttcaaataag tacccgatcg
    45481 tacaaaaatg acctaaagca atatcatttg atccagtgat tttacgaatg atcggttgcg
    45541 ctactgccgg catagcaatg gcgaaacaag ctccgataaa accaccgacc aagatcaata
    45601 cgatccctcg cagaccggca aaatagccaa agactgtcgt catcgttgcc atccataaaa
    45661 ttgcttgacc agttaaaaaa atgtacttcc attttgtgaa acgagccaaa ataatattga
    45721 atacaaaaat ggctaagaac gtcagtgcaa tatctctacc taaacccaaa tcattcattg
    45781 cttgaccatt gatagattca atagaaggga tgatcccttg catatgaaat ccttcagtaa
    45841 agattttccc gaaataagtt aaacttccaa cgatgatgct cgaaccggca ctcaacactt
    45901 gaaatcctag gagtgtcttt aacgtgccag agatgacttg tcctgctgat ttcttctgca
    45961 gtagtagacc caacatagca atcaatgcaa tggtgatcga tgcctgagtc aaaatatttt
    46021 cgatgataaa attgataacg cccatgtt
//
LOCUS       DACDIG010000016        45667 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.016, whole genome shotgun sequence.
ACCESSION   DACDIG010000016 DACDIG010000000
VERSION     DACDIG010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.016"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            6..245
                     /locus_tag="IV247_000926"
     CDS             6..245
                     /locus_tag="IV247_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777014.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            312..521
                     /locus_tag="IV247_000927"
     CDS             312..521
                     /locus_tag="IV247_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777015.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            581..1252
                     /locus_tag="IV247_000928"
     CDS             581..1252
                     /locus_tag="IV247_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP8777016.1"
                     /translation="MKKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPGYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            1305..3281
                     /locus_tag="IV247_000929"
     CDS             1305..3281
                     /locus_tag="IV247_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP8777017.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGGLSAVVYFKKKA"
     gene            3294..4049
                     /locus_tag="IV247_000930"
     CDS             3294..4049
                     /locus_tag="IV247_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8777018.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFCVPIIFIYKKIRRRSE"
     gene            4046..4870
                     /locus_tag="IV247_000931"
     CDS             4046..4870
                     /locus_tag="IV247_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777019.1"
                     /translation="MNKRKWLFVCVAALFSTVAYSQLSFADSSDSSTTQTIDNSITAP
                     SETDSSENTTEETADSNISFTESTNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIIS
                     SNQINNQGSQVAQQEGINGAKFIVYDITNILEEMSQDDNEKNQSVEIIESKLKDRAKK
                     LSYDQLKKVAEGETKTIDDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLL
                     TPVSNEKGEFLTDVWLYPKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            5159..7270
                     /locus_tag="IV247_000932"
     CDS             5159..7270
                     /locus_tag="IV247_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP8777020.1"
                     /translation="MEGIMENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQ
                     IKENNNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAH
                     YQKVGTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQ
                     SINVMNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQ
                     QRVSWYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSK
                     VVGGRAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTAL
                     LWTPQPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKS
                     FKITDTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSF
                     LANAANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPN
                     KKVEQDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQD
                     PVEDRLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREI
                     QLIFTAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLT
                     VIGSILFLTLASLGYVVLKLK"
     gene            complement(7308..9365)
                     /locus_tag="IV247_000933"
     CDS             complement(7308..9365)
                     /locus_tag="IV247_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAP8777021.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYMVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVDILKEQIINVTHGESVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFSTPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(9479..9691)
                     /locus_tag="IV247_000934"
     CDS             complement(9479..9691)
                     /locus_tag="IV247_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777022.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            9878..10345
                     /locus_tag="IV247_000935"
     CDS             9878..10345
                     /locus_tag="IV247_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777023.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSELNKYKLQKTSE"
     gene            10342..10584
                     /locus_tag="IV247_000936"
     CDS             10342..10584
                     /locus_tag="IV247_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777024.1"
                     /translation="MIASLFIYPEGGITVAKVKTPFEKAASKILERRGVSEIQWKKEV
                     LEKAQFDLLRGKDKDLEEYIYQREREKLVFDEIIKH"
     gene            10602..10904
                     /locus_tag="IV247_000937"
     CDS             10602..10904
                     /locus_tag="IV247_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777025.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            10976..13030
                     /locus_tag="IV247_000938"
     CDS             10976..13030
                     /locus_tag="IV247_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777026.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLIDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDFQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELGQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKVKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSESNE
                     VK"
     gene            13030..15642
                     /locus_tag="IV247_000939"
     CDS             13030..15642
                     /locus_tag="IV247_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP8777027.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSLENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            15639..18170
                     /locus_tag="IV247_000940"
     CDS             15639..18170
                     /locus_tag="IV247_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777028.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PEQAKGMAGNDKSGLSDIQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            18222..18554
                     /locus_tag="IV247_000941"
     CDS             18222..18554
                     /locus_tag="IV247_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777029.1"
                     /translation="MDKYSNMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            18555..19148
                     /locus_tag="IV247_000942"
     CDS             18555..19148
                     /locus_tag="IV247_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777030.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            19311..21005
                     /locus_tag="IV247_000943"
     CDS             19311..21005
                     /locus_tag="IV247_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP8777031.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEIVKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            21693..23570
                     /gene="ltrA"
                     /locus_tag="IV247_000944"
     CDS             21693..23570
                     /gene="ltrA"
                     /locus_tag="IV247_000944"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP8777032.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            23701..24078
                     /locus_tag="IV247_000945"
                     /pseudo
     CDS             23701..24078
                     /locus_tag="IV247_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            24078..25283
                     /locus_tag="IV247_000946"
     CDS             24078..25283
                     /locus_tag="IV247_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP8777033.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
     gene            25299..25943
                     /locus_tag="IV247_000947"
     CDS             25299..25943
                     /locus_tag="IV247_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777034.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            25954..26760
                     /locus_tag="IV247_000948"
     CDS             25954..26760
                     /locus_tag="IV247_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777035.1"
                     /translation="MSKKKGERQRFKKRKSYLRWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            26782..27012
                     /locus_tag="IV247_000949"
     CDS             26782..27012
                     /locus_tag="IV247_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777036.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            27558..27833
                     /locus_tag="IV247_000950"
     CDS             27558..27833
                     /locus_tag="IV247_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777037.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            27875..28297
                     /locus_tag="IV247_000951"
     CDS             27875..28297
                     /locus_tag="IV247_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777038.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            28316..29908
                     /locus_tag="IV247_000952"
     CDS             28316..29908
                     /locus_tag="IV247_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777039.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            29920..30255
                     /locus_tag="IV247_000953"
     CDS             29920..30255
                     /locus_tag="IV247_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777040.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            30402..30596
                     /locus_tag="IV247_000954"
     CDS             30402..30596
                     /locus_tag="IV247_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777041.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            30628..33000
                     /locus_tag="IV247_000955"
     CDS             30628..33000
                     /locus_tag="IV247_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP8777042.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSSEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            33061..34521
                     /locus_tag="IV247_000956"
     CDS             33061..34521
                     /locus_tag="IV247_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAP8777043.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFIPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            34532..36736
                     /gene="topB"
                     /locus_tag="IV247_000957"
     CDS             34532..36736
                     /gene="topB"
                     /locus_tag="IV247_000957"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP8777044.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAESIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ENDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            36975..37568
                     /locus_tag="IV247_000958"
     CDS             36975..37568
                     /locus_tag="IV247_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8777045.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKDAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDQYAIQPHYTNKENFDLGVTETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            37584..38483
                     /locus_tag="IV247_000959"
     CDS             37584..38483
                     /locus_tag="IV247_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP8777046.1"
                     /translation="MTPTQFKEKTRNFAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTANPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERIDEKNLYKSVQGTFKE
                     RNNQSVFKHLYDQEMPQLVKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKAERKQNFKRSQQMNQEMER"
     gene            38486..38971
                     /gene="ssb"
                     /locus_tag="IV247_000960"
     CDS             38486..38971
                     /gene="ssb"
                     /locus_tag="IV247_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP8777047.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFSLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            39337..39597
                     /locus_tag="IV247_000961"
     CDS             39337..39597
                     /locus_tag="IV247_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777048.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            40038..40244
                     /locus_tag="IV247_000962"
     CDS             40038..40244
                     /locus_tag="IV247_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP8777049.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            40244..40495
                     /locus_tag="IV247_000963"
     CDS             40244..40495
                     /locus_tag="IV247_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777050.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            40510..40947
                     /locus_tag="IV247_000964"
     CDS             40510..40947
                     /locus_tag="IV247_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777051.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFESIEKNGAFYTIESEEKDNE"
     gene            40940..41647
                     /locus_tag="IV247_000965"
     CDS             40940..41647
                     /locus_tag="IV247_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777052.1"
                     /translation="MNKVVYQLTTEDVSQFLTSKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGK"
     gene            42028..42207
                     /locus_tag="IV247_000966"
     CDS             42028..42207
                     /locus_tag="IV247_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777053.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            42548..42811
                     /locus_tag="IV247_000967"
     CDS             42548..42811
                     /locus_tag="IV247_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HAP8777054.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            42805..43155
                     /locus_tag="IV247_000968"
     CDS             42805..43155
                     /locus_tag="IV247_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP8777055.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(43179..43793)
                     /locus_tag="IV247_000969"
     CDS             complement(43179..43793)
                     /locus_tag="IV247_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8777056.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRL
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            44107..44529
                     /locus_tag="IV247_000970"
     CDS             44107..44529
                     /locus_tag="IV247_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777057.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            44539..44742
                     /locus_tag="IV247_000971"
     CDS             44539..44742
                     /locus_tag="IV247_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777058.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            44953..45537
                     /locus_tag="IV247_000972"
     CDS             44953..45537
                     /locus_tag="IV247_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8777059.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT        16748 a         7210 c         9266 g        12443 t
ORIGIN      
        1 agtagatggc gcgaccagtt ttagaggcga tggatatgga tactttgaca aaaggaaggt
       61 atgtacagct gttagctcgt ggttattcac ctaaagaagt attcaaagcc ttatctaaag
      121 gaacagatat ggaaaaagaa agattgcgaa aagaattcga ttattggaga acccataatg
      181 gaatcaagga tttgaaacca gcacggccag taattaaacg aaagaaaaag ataacccaag
      241 actgaagtgt agagcgtatg tgatcatagg cttttttatt tttgcaaaaa acaaggaagg
      301 taggaaaact catgaattat gaagaaatcg aaaacagaaa aaaagtgtcg aaagaaatgg
      361 aagaaaagtt actaaaaacg atgaagcaaa aacaccttaa aagattatct gtcgcgcaat
      421 atatcaatga tatgcagtta acaggaaaag agaaagcttg tttgttaggt tcaatgaaaa
      481 actttgaaca gttgagacga acttatagaa ttcattttta aaactttttc tatggaaatg
      541 agaaaaagtt tttttgtttt gtctgaaagg aggcagatgg atgaaaaaat ggttatttgg
      601 ttttttagga gtcactttaa tcgtcttttg cagtgtgttt ggctatgtta gctaccaaaa
      661 acatgaagga gaggtgttta agcaaaatat agaaaaaaag atgcccgtag atcaaatcaa
      721 tgcacatgca aagagttata aggaggatgc aacgaacgtc aataatgata tgtctttggg
      781 gcaaatgttg tccatacaaa aagaagcaat cgaaatgggc gtcaataaac aagtatttgc
      841 acaaattcag attcctgcac ttggtttagc attgccaatt tttaaagggg caaatcaata
      901 cactttgagt cttggagcgg ctacttattt ttatgaagat gccgaaatgg gcaaaggaaa
      961 ctatgtccta gcaggtcata acatggaaat gccaggtgtt ttattttctg atattcaaaa
     1021 gttgtcatta ggggaggtga tggacttagt tagtaacgat ggcgtctatc gttacaaggt
     1081 aacgcgtaaa tttatcgtac ctgggtactt taaattaatc gatggcgttc ctgaagagaa
     1141 ctctttcttg tctttgccaa agaaaggaga gaaaccctta ttaacgttgt ttacttgtgt
     1201 ttatacgtca caaggaaaag aaagatacgt ggtacaggga gagctacaat aacaaggttt
     1261 taatcaacgc gagtcgttga aatatgaaat gaggagagta aaaaatggca ataaatcggt
     1321 atgtaaaaaa agctggaatg cttgttggaa tgttaggaat cattgcaggc tgctttcagg
     1381 ggtatcaagt acatgcagaa gatgtcacgc aaaaaacacc accagagaag gtcaatatca
     1441 cggttcataa attaatgtac gatcaaggaa cgcaattgaa tgttgatatt gacgggatca
     1501 aaaacgatgg ttatacgcac gatcaatatc ctacaggtgt aacgaaatac aacaaggcag
     1561 attatggtga tgttgagttt acgttaggaa atattacgga tcaagtatta ccgagagaag
     1621 attctgatct aaccaacgcg aaagtggacg agattgtaca agatgtagaa gacaagggat
     1681 ctaacagtga gtacgtcaag aatgcaacca acaaaattac ttcagctgta gatgaaaatg
     1741 gtgagatcac ttttactgat caaccagcct atgtcaattc caaagggaat gtatatgtgg
     1801 tttatgaaag taaatccgct gctggattag tcacacaaaa agcaaaacca atggttgtga
     1861 ttgcgccaat gacagataaa acaggatcag gatttttaaa aaatattcat ttgtatccta
     1921 agaacattgt ttctaagttg agttttgaat taacgaaatt tggtgacgat ggcgcagacc
     1981 aaagcaaaca aaccctttta aaaggggcaa aatttgagtt atacaagggc gaacctggaa
     2041 aaggaacaaa attgggtgat cttgtatcgg atgatcaagg aaagctgact gcgactgatt
     2101 tgacgttagg aaaatactat tttgtggaag ttccttcgga agttgtagtg ggatcagatc
     2161 aagaaccaac agcagatcaa tatttattag gcgcagacgc tcgcaacgat gcgcacaata
     2221 aattaacctt tgaaattacg aatgatggcg tgacaagtga cttaaaagct tcatacgtaa
     2281 actataaagc accagtgatc gataaaacag taacaaatgg aacaggacaa gaacattctt
     2341 tccaaattgg tgacgcagta aattatcaag gaacgattca tattccaaca gacattgcag
     2401 gtggcgcaga cggtatcaca gtaaacggtg tgaaaagcga aacttctcct tacagtgtat
     2461 ttaaatgggg agatacagca ggtaaaggac tttcttatgt tgctgaaaaa gcagatatta
     2521 aagtaacaaa taatgatggt tcagtcgtat taaaagaaaa tactgactat aaaatcgaaa
     2581 attctgatag tggttttgtg atcgatttta tcgtaaataa tggacaagta agtgatacag
     2641 ttgcgaattt acatggtcaa gatctaaaaa tgacttataa catgtttgtg aatgatagtg
     2701 ctgaaatcgc aaatccatta acaaatagcg tggatttcgt ttataacaat aatccattta
     2761 atcaggaaga acaccacgaa aaaacgaaag ctgacgttgt gacttatggc gcaaaattct
     2821 taaaagtcga tagcggccta tttggaacag gtatcaaagc aacaccgctt gaaggcgcag
     2881 aatttgcagc taagaatgca gaaggtaagt actacggtgg acttactgat acagataaag
     2941 atggtgtcaa agaagcagtt tgggtagacg atgtagcaaa tgcagcaatc ctaaaatcag
     3001 ataaagaggg tcattttgaa atcactggtt taactgaagg agattactca ctggaagaaa
     3061 caaaagctcc tgaaaactat caaaaattaa caaaagagat ctctttcaaa gtagacaaag
     3121 attcatacaa agaagaaaac cgaatcacga tcaaaaataa tcaaaaagct tccgtaccaa
     3181 tgactggtag caacggtttc caatcttacg tattgatctc ttgcttgttg ctaggagcag
     3241 ggggactttc tgcagttgtt tactttaaga agaaagccta gaaggatcag ctaatgatta
     3301 ttcgtaagcc cccaaaaaga agagtcatgc acaaactgat gatatttttt tggttgctgt
     3361 cggcagtagc agtctattta attccagttg tggaaacaaa tgtagcgaaa tttcaacaac
     3421 agcaaagaat tttagccatt gaaaatcttg aaaaaacaga ttcttctttg gatgttgcga
     3481 aaatagagtt aggcgatcct gttggtgttc ttacaattcc aagtatttct ttaaaactac
     3541 caatttatga cggaacttcc gacaaaattt tagagaatgg tgtagggatt acggaaggaa
     3601 ctggcgatat tacaggagga aacggtaaaa atcctttaat cgcggggcac agtggacttt
     3661 ataaagataa tcttttcgat gatttgcctt cagtaaaaaa agggcaaaaa ttctttatta
     3721 aggtaaatga agaacaacat gcctatcaaa tttatcgaat tgaagaagtt aaagcagaag
     3781 aattacaaaa aaattattta gattatctta tgcctgaaaa agaccaagat aggatcacgt
     3841 taatgacatg cacaccaaaa ggtgtcaact cacaccgctt tttagtgtat ggtgaacgag
     3901 tttccttcag taaagaagat ttaaaactac agaaaaaagc aaaaaatagg aaatcaattt
     3961 cattggattg gcgattagca ctgctattcg cttttttttg cgttcctatt atttttattt
     4021 ataaaaaaat aaggaggaga tcggaatgaa taagagaaaa tggctgtttg tttgtgtagc
     4081 agctctcttt tctactgtag cttattcgca actgtctttc gctgacagca gtgatagtag
     4141 taccactcaa acaatagata attcaattac tgcaccaagc gaaacggaca gttcggagaa
     4201 tactacagaa gaaacggcag attcaaatat aagctttact gaatcaacga atccaacgtc
     4261 agaaagttct tcagagactt cagaaaagcc gcaagacgta gaagatacgc aagtgatcaa
     4321 gcaagccatt catattcaaa aaattatttc ttcgaatcaa atcaacaatc aaggcagtca
     4381 agtagcccag caagaaggta tcaatggcgc gaagtttatt gtctacgata tcacgaatat
     4441 cttagaagaa atgtcacaag atgataatga gaaaaatcaa tctgtagaaa taattgagtc
     4501 aaaattgaaa gatcgagcaa aaaaactttc atacgaccag ttgaaaaaag tggctgaagg
     4561 agaaaccaaa acgattgatg atcaggaagg cgtgattgaa ttttccattg aagtacctgc
     4621 aaacaaaaaa caagcatact atatcgttaa tgaatcaagc cctgaaaaca tttcaaacag
     4681 tgaagatatt gttttattga cacctgtttc aaatgaaaaa ggtgaatttt tgacggatgt
     4741 ttggttatac cctaaatcag agaaatcaga gccgaaagaa gaaattaaaa aagttgtgtc
     4801 aactggtgta aagaagaact tttttgaaaa ttgttgggaa ttttttgctc aattattttt
     4861 tgataattag gatgaaatga tgaaaataat aagaatatta gtttttaatc tatccttttt
     4921 agccgcaaca atattgtgtt gcggtttaat tttttctcaa ttatcaattt atccaagata
     4981 tcaacgattg ttcattcatt ttctaaacat tcaatgtgct tccatgccat tagaggtgat
     5041 taaaaagctc ttgatcaatc caatttttat ggttagtttg gttcttctta caggattggt
     5101 aacgtgggta ttaggaaaaa aagaatgaaa agcttacgat cgtttaaaaa aatattagat
     5161 ggagggcatt atggaaaata aaattcattg cactacggga agagtaatta ctttagtgtt
     5221 gtttttgttc cttcttcttt ttgcgagggg aactgcagtc caggccttag aaaaagttga
     5281 tccgagtcaa atcaaagaaa ataataatct ggtgctttat catgatagtg attcattgag
     5341 aaatatcgaa gtaactggga tcaatggatt taagatacta gatgagtatt caactaaaaa
     5401 acaaaaagta cttgcttggc caaaagtcaa agatttgaaa agtacgtcaa aaatcgttgc
     5461 ccattatcaa aaagtaggta cttatctagg aaaagaaatt gattgtgacg tgactttttc
     5521 taattttgta gcgcacgctg gggttaacca agacggatta gctaaaacaa ttaatggcaa
     5581 aaagtattat aattatttgc tctttgataa aaatatttat cgaggatttt tcatgcagtc
     5641 aatcaatgtc atgaatgcaa aactagaatt taagtatgca ggaacaaaaa caaaagtagc
     5701 tttgagtgaa ccacagccag aaattcaatc aaaaatgaga tcgatgagag cagctggtat
     5761 tgatttaaac gatacatttc tatccattta cagtttaaat ggacagtatc agcaacgtgt
     5821 gtcttggtat aatgacaata atgaatctgt cggtgaacgt aatattgata tgagagaatt
     5881 tgttgggtat gaaaaaatgg gtagcttacc ttattttgtc acaacagata cagcatgtgc
     5941 agaatacaaa gctcctgcgt tatcaacaaa caatttaact tcaaaagtag tgggaggacg
     6001 tgcagaaaag gcttatagct cgaatgatca tttcaccgat gttgtaggag ctgatactta
     6061 tcacagaagt ggtgtaacgt atacgcttca aggcgcttcc ccaacattca tgattggcgc
     6121 aaatacgaat agtatgatgt ttagctttga tactgcattg ctatggacac cacaaccatc
     6181 gaagcctaca aaagaagtgt ttaacaaagc taatactgaa gaggcagcac acaatattga
     6241 caaaaaagtg attccacaag gatcagatgt ttactatcat attcaccaaa agtttgatgc
     6301 attaacagtc aacacaatga acaaatacaa atcatttaaa atcactgata cctttgacag
     6361 caaaaatttt gatatggtat cggatgggaa aaactatgat ggcgcattgt ggtatgttga
     6421 agcgaatggt acttggaaaa aagctgatac aggaggaaag ttaactgtat caggtaatac
     6481 agtcacttat actgcttctg acagcttttt agctaacgct gcaaattacg gtagggatta
     6541 tattttgcag ctccatatga gagcaaaaaa tgatgcaaaa ggaattgcac caaatgtggc
     6601 tactgtaacg tttaacaatg atccgaacaa aggaacacaa aaaacaaata cagtagaaaa
     6661 ctatttctct cttaaaccaa ataaaaaagt ggaacaagat gggaaagatg ttgatggtcg
     6721 aaatgatggt aaaaaaggaa cgcctacagc tcctttaaat gcaggatctg aagtacaata
     6781 tctagtcaca caaaaatggc atacaaaagg tgtagacatt gcctcagatc attacaagca
     6841 atttagtatt caagatccag tggaagatcg tttgacgtat aaagaaggtt ctgcgcgagt
     6901 gatagatatg agtacaggaa aagatattac ttcagaaggt acactaacct atgatagcaa
     6961 ttcaagaacg ctgaagtggg aagcttccgc tgatttctta agtaaaaatc ttttagatgg
     7021 acgagaaatt cagctgatat ttacagctaa aactcctttg aatgaagaat tggatatcga
     7081 caaccaagca aaagtttcag tagaaagtat tgagaaagca actaatgtag taacgattgg
     7141 ggtaaaacct aacttacctc agatcattgt gccaaaaact ggatcgaatc atttagtgct
     7201 aactgttatt ggaagtatat tatttcttac tttagcatca ttgggctatg tagttttaaa
     7261 actcaaataa atataaaaac aaggctgaga atcagccttg ttttctttta tttgaagatt
     7321 ggattcgcac taataaatac attatctacg ccaattctac cagcagagtc aactacaaaa
     7381 ttatgaccta atccgagaat actttggtta ttataaagta aatttgcatt atgggcaggg
     7441 ctatttttcc atcctaccat ggcatcgata gcaccatcgt ctgataaaag gtcatcaata
     7501 tacagcccag tttgacttat gttttctccc ttagcaccgt agcagcctaa gctttccaat
     7561 aaatcttgga aagtaccacc accagggcga tagtgactga agctgacttg taattcttta
     7621 gctcgaatat ctgcagcctg ttgaacaata tcattgaatt gcattggggc aacaccattt
     7681 tgttggcgat aatcatttaa ccaagcaagt tctttttgac gcataaagtt acgtaattcg
     7741 tctaatgaaa tctttttata tttgaatgtg attgttttat tttgatcgac gttattaatg
     7801 gatcgttggc cgtttttatc cacggtaacg gtaggatcgt catttctgca caagaaataa
     7861 ttttgatcag tcatattttt ccaaggagta gagaagctat ccccttcgta tactgttttt
     7921 gattcactag agagaacagc gttggtatca gcatttacat gattaacagt cacattaaat
     7981 ttacgagtgt agttgaaaat ataattttga ctttcagtca cattgtttaa ggttactgaa
     8041 tttgttggtt ctacccgatc gtctaaaaca tatccttgaa tagaaggagc agttaaagtg
     8101 acactttcac cgtgagtgac gttgattatc tgttctttca aaatatcaac tcgcccctca
     8161 tttttgtagt tgttattgca caacaaattg atttgatgca cttgattata attgaatgtg
     8221 attgttgtat tacctgtgat tgttttgctt tgagtatcat taccaatcac tttgtagcta
     8281 aaattatctt tagcaccttc gctatattta aatgcttttg ccttagcagt gtaagtagaa
     8341 ccagaagtca cttttgcttg atctgtagca atcacttgac ctgtttgatt gtcaatatgg
     8401 ttaatggtaa ccatatattc ttttggcgtt tcaggtttgt gttgcaaccc tttaaatgca
     8461 gcatcaagac tatttttagc attgtttact tgtgtttgag aaaccttaga atcgtctagt
     8521 acttttttag cattggataa agcagtctga aaattattcc aagattcttc tgtaaaatct
     8581 cctttagcag ttgattttac tcggttgtat aaagcttgta attcagcttt attcacagta
     8641 ggaactggtt ttttctttaa tcctttgtaa gcagaatcta atttattttt ggcactatcc
     8701 acttgctttt gagtcgcttt tgagtcattt aacactttct tagcattgtt taaagcggtt
     8761 tggaatgcgt tccaggtatt gtcggtatag tcaccttttt tcgttccctt aacgctcgta
     8821 taaagagctt ctagagccga tttattcact ttgggtttag aggtgtcagc gccataccaa
     8881 ccgatacctt cgtatttcca gccatagtct ttggttaacc attgtacttc tttccaactt
     8941 gcagtgtagt gatgtgagcc agattttaca ccaggaagga aaaggcggta gatcggttta
     9001 ttattttttt cggcagaacg ccaggcaggc ccttcataag tccagccgta atttttagtc
     9061 aaccatttca cttcatccca actagctgtg taatggtgat cacgaagacc tttattgtac
     9121 aaacgataga caagatggcc accgctagtc ggtgcttccc aggcggggcc ttcataattt
     9181 ccccatttta tctttactag atggtcacgt tcttttttgc tagaggtgta aaaatgttca
     9241 ttattatttg gattgtaaag gcgatacatg acgttagttg aagcagcgtc aacagatcca
     9301 gcaagactaa gtgaggcagc aaatgttcca atagctaaaa cagtttttgg attaaataat
     9361 ttcatttttt caactcctaa atttaattaa aaatgtttac atgattatta tatatcggct
     9421 aaccgatata gtcaataaaa aataagaaaa gccttatata attttggctt ttcttatttt
     9481 atttgttagt tgaagtatta atatcaacga tttcttcagc caatttattt aatttcatag
     9541 catttatgaa tctcatgttg tctatactag agcgtttgtt ggcaaggtca cttactgtag
     9601 ttaagggtac gccagattct atggatatag aacgaactga tttaccacta tcaaacagca
     9661 accattcgat tttcttttta tcagcgtaca tttcaatgct ccttcttgtt ttttctttat
     9721 tatagctctt ttttgtaatg aatcacaata tcaagagaaa aattttctat agaaaggtgg
     9781 atttttatga gtattcgttc tccgtgtatc caaatgaggt ttgttgctac tttcgttatt
     9841 tgatatacta aaaataaaca acgaaagaag gaataaaatg aagaaaaagc aactatggtt
     9901 tgtctctatg tttgcaatat tttttattat tttaagtgga tgttcaagta ataagaaatc
     9961 ggaagctaaa gaagataata gtttattgca accaggaact gtttggaaag aagaagtagg
    10021 tggtcttatt cataatttaa aaattataga tgatactacc tgggaatatt ctgaaagtgt
    10081 atggcatcca gatcctgtac aaataacagt taagaggcaa aaagactatg aaggtttaga
    10141 aagatacaaa atagtagatt ctgcaggcat tagggaattt ataaacaaac ctgattcatt
    10201 atttattgtt gttccctatg agaaggatgg cgtaaaaaaa ataatttttc ttggatctag
    10261 caaggatgag aagcgaacga aagagaaatt aattcatgat ggatcagagt taaataaata
    10321 caaattacaa aagactagtg agtgattgct agtcttttta tttatcctga aggaggaatc
    10381 acagtggcaa aagttaaaac accattcgaa aaagccgcgt ctaaaatctt agaacgccgt
    10441 ggtgtttctg aaatacagtg gaaaaaagaa gttcttgaaa aagcacaatt tgacctatta
    10501 cgaggaaaag ataaggactt agaagaatat atctatcaac gtgaacgtga aaaacttgta
    10561 ttcgatgaaa ttattaaaca ttaaaaggag cgaataatct tatgtttgaa tcaattacaa
    10621 tgaatttttt aactcaaccg aaagtagtag ctgcagttga tatttttaga gcattacaaa
    10681 ctgccgcaac tagtttgaaa aataatctga tgatttttgc tgctgttgcg gcagtggctt
    10741 ccattgctct tggaggtatt ttcttcttct ttgggcgaag aggagctgaa aacgggaaga
    10801 cacacgtagg ttatgtattg ttgggggtag gagttatttt agctgccgct tctattgtag
    10861 tttcaatgtt ctcaatgttt ggacaaaccc cacaaggact ataaaaaaaa caagtaggag
    10921 gagtaaaaag tacgtggcta ctttttactc ctttttttat gaaaggagaa tattcatgtt
    10981 ttttacgatt tcaggaaatg caaaattgga taaagagtta ataagaaaag caacgaagaa
    11041 agtgggaaag cgtacttcct ttttaccgaa aacaattaaa tattcggtca ttgaaacgga
    11101 agaacagcta catgtgttag gacaagtgtt agaacaagag aatcaatctt ctgttaaatt
    11161 gactctcacg ttacatgata aagaaggaaa aatagaaaag gctggcacag aatacgtttt
    11221 tggcccactg gattatcaaa tttcagaagg tttttatcct acattgatag atttaatccg
    11281 ccttgatgtg tttaaagata cggatgaaac tatgagtgtg gaagagtctt atcgaaacga
    11341 aaaactttta caagttttag aagaagagtt agagagcgac caaagaaaag tgaatcaagt
    11401 gagtgaagtc gaagtaaatg aagaagtacc atgggaacga ccaatatttc caaagaagga
    11461 gtcaaaagaa aaagagacag tctatgttga cgaacaagct gatattccga ctcagaaatc
    11521 gatatttgag gaagagaacg atgtgatggg tcaattggag gtagatccct ttgaagaaac
    11581 aatggaggat gaagagacgg acgaagtaga agttgatcca gttgaagcta atgaagagtc
    11641 aacatggact gaaggggatt ttcaggaata tattgaacca cttatcagtg aagaagtaga
    11701 aaaaaacgaa acagagatac aaaaggcaac tgtatttgat tatattttgg atcaatatcc
    11761 gccagagcat gaatggatga aagaaaaaaa caaattattt ttaaatgagc tttattcgaa
    11821 atatcaatta ccccaaacct atcaagcatt tttagagagt aaagaagaat taatctcaca
    11881 gggtaaaggc acacttttag aacagttaga gtctgtccaa tcaacaaatt ggcatgagct
    11941 tggacaagaa gatttattag ccacctttga agaacgtcaa caagaaacag atcaagaaat
    12001 taatgagtat gcgacggaac agtcgtctaa ttgggagaaa gtaaaagagg aactaaataa
    12061 agaagaacag cgagtgattg aagaagaagt acaaaaagtc aaagagcggt acgaaaagaa
    12121 acgcgacaat gcttattcct tgtgtgtaga aaagatcaac aattttacta agacgaatca
    12181 agaagcgtta tcgaaagaaa aagaaaactt gttaaaagaa cgtattaaag aacgaaaaga
    12241 agagttctac cgcggtttac gtacagataa gctaaagatt agcaaagaat taaatgaaca
    12301 actgaataag ctatatcaaa caacgactga agttcttatg gaaaaacacg aacagatcca
    12361 gcaagaatta caagtacaaa ttccaaaatg gaaaaaagag tatgaaattc aacaacaaga
    12421 attgaaagaa caagaagaaa aacaacggca aaaagaaaaa gagcgtgaac aattagaaat
    12481 aagaaaacgg gaacaggatc ttgcggaaca aaaaatggcg atagaaaaac aacggttaga
    12541 gcaagaaaag gaaaaagaag cagctgctcg taaggaacgt caagaacaaa tggatacatt
    12601 gcgtcaagca cagttgaatt atctagcaca acctcaacca gtagctgctg ttcaaacgcc
    12661 agtgaacaac caagcaaatt taactccttc accccagcgt tcctttaacg gatggatggt
    12721 gggatgtatt gcaagtttaa gcttgcttct aggtggagga acagtatttg cttttaatca
    12781 tatcaatcaa tcttcacagg ctgattctgt agccacaacg gcgtcaatca aggaagaagc
    12841 ccaggaacaa gcaagaaaag agtatgaaca acaattagaa gaattattga atcgtaccaa
    12901 tcagtcaaca acagagtcaa gcatggaaaa tagcaaagag agtgattcta gtaaggctaa
    12961 agaaggatca agcgattcaa ctgtacaatc aaacacttcc gaaagttcag aatcaaatga
    13021 ggtgaaataa tgcgtgttag agaaacaacg aagcgaaaag acaaacgatt caatttagaa
    13081 aagaataggt atctttctca tttctatcgc tatactccct caggagaaga gctaccagaa
    13141 aaacatgaca aacgcatttc gaaaaagatt ttttatagtg gacttgtata cctcccttta
    13201 gagtatttag taacaagagt accagaagtt ttcaaccgat tggaaattgt taagggaagc
    13261 ttacaaggaa taaatctgtt tcaattacag gaaatgccac aggtctttca ttttgattca
    13321 gctggttttt ggaatccatt tactcctaca actagtttgg gtacacgttt agcaatttcg
    13381 acagtttgtg tggttccagc tgtacttatg ggattgaaaa tggacatgtg gtttcgtcct
    13441 ctagcagatg gacaaaaagg ggataatcga ttaatgacct taaaagaaat ccaacagatt
    13501 tatccgcaaa tacctgaaac aaaaactact tttccaggtt atggaggtat tccaatcaca
    13561 cattacaaaa aatattggta cattcaaaca gatactgtta atacgtgtat tgttggtacc
    13621 tctcgatcag ggaaaggtca gattgaagtg ttagccacca ttgacaattt atctcgtgca
    13681 gaaaaacaaa gttcaatggt cgttaatgat ccaaaaacag aattatatgt tggtagtaaa
    13741 gatgaactag aagctagagg gtatgatgta tatgccttta atatcaccga tccactgcaa
    13801 tcaatgagtg ataatccatt ggcactcatc gtgaaatact ggaaacgagg agacatagat
    13861 actgcaacac agttaaccaa tactttcaca aatacgattt attatgatgc caacgcttca
    13921 gataacaaat actttaacga aaacgcgcag aaagctgtca atgcgcttat ttttgcgttg
    13981 ttagaacaag ctgatcaaac aggagattac tccaaattga caccgaataa tctcgttgag
    14041 cttctttctg agattggcgg ttttaattat tatccagatc cagacaatga attcaaacag
    14101 ttgaatgcct tagatgaatt tatgaatcag ttgccacctg gaaacattgc aaaaaaacaa
    14161 tatggggcaa cgaaaattgg atctgataaa gcgaagggaa atattttatc aacggcaatc
    14221 acgggattga atccatttac tctaacgaaa attgcaaaga tgacttctca atcaacgatt
    14281 gattataaat cggttgggtt tccaaaatac ttagatttga aattacatga aagtctttta
    14341 aatcaacgag tcgtagtaga attttatcat aagggaaaga aatttcatga agaacgtgtc
    14401 aaagtaggct ataaaggatt tagtgaaatc aattttgatt gtcatttagt tgcaggtgat
    14461 caagtttgtt taaaatacga tcatcaagga aaagaatatg tggcttggta tgaattttct
    14521 caaagagtat tgaaagacga agagggtaat atcgtttatc ggaaaaaacg aggagaaacc
    14581 catttgccag aattagaaaa agaagccgtt ctaacattag atgaggaaaa aagtaactta
    14641 ttagtagagt ctattcggat gcactatacc gataaaccaa tggctatttt tatgcttaca
    14701 cctgattatg atccatctaa ccatatcatt gttagtattt ttcttagtca gttatataaa
    14761 gaattatcta cgcaatgtgt taagactaaa ggagataaat gtcaccgcag gattcatttt
    14821 attttagatg aatttgggaa tatgccacca atggataata tggaaggaat catgacggtc
    14881 acagctggcc gaaacatgtt gtgggatctg tttattcaat cttatcagca actttatgcg
    14941 aagtatggag aacaaaacgc agcgattatc aaagaaaatt gtcagacaca tatttatatt
    15001 atgtcgacag atgataatac catcgaagaa ttttctaaaa aagtggggaa taagacggtt
    15061 gaacaagaga acagtaccat caattcatta ggtatgaata ctcatatcaa tcgcaatgtt
    15121 gattcagatc gtatcttaac tcctgaaaga atttctactt ttttagaagg agaaacgatt
    15181 gtactcgctc ctttaaaacg acaagatttg aaaggtcata aaatacggcc gtatcctatt
    15241 tttaatcatg gggaaacgaa tcttccttat gcttatcaat atattgaaga tttcaatcca
    15301 tcgtgggata tcaatgatat agacatacat tgtacacatg cggacttgga tcttaaggct
    15361 ttacaaattg attatgatta tttcctacca gatgaatatg ctagaaatct ctatcatgga
    15421 ctgcatgatc aattagaaga aaaaaacgaa gaaaatatgt tttataaaat cgttcaatct
    15481 cttgagaatg agacggctaa aaaattgtta attcaatttt acgaagaaca agcagcagat
    15541 cgacttcaag aatatgttga aaagttgatc aaaaataaag aaatttctga aatgattggg
    15601 aaacggttgt tattggaatc tgaaaaggtg gtgaaagaat gatcattgga ggaatttttt
    15661 ctggcatagc cgactggtgg tcaggcttat tcggagaagg ggatgcaaac cccaaaaccg
    15721 taacgaaagt cttacaagat tatgggaatt atctgcagta tcaagatgta tttagttgga
    15781 tttggaacac ttttctgtgg ggattgatta aaggattata taatctcaat actttactag
    15841 aaaaaaccat ttatcaaagc tttgatttaa aagacttatt gaacgccgct ggggtaaatg
    15901 cactatttca aagtttgatt agtaaggtat tggctctttt ttgtgtcgtc acattaattt
    15961 atttaggctt gaaatttttt atgagtaaac acccaccaaa agtcaaaaat atcttggtta
    16021 atttatttat ggccatgatc ttgatttttg gtggtagttc tttaatcgat gaagggttaa
    16081 atatcagtaa aagcttttac ggagacgtca ctgcagcaaa taaagcagat aaaacgggat
    16141 cacctgcttt tcaaattatt caaaataatg tgatcgatgt cagtacgtta ctagcaactg
    16201 acccgaataa agtagaatcc ctaccaacag ataaaagaaa tgctttaaca gcgaaaaatt
    16261 ttaaaacagc gaatatcaat gggattatta ctcctgaaca agctaaaggt atggcaggta
    16321 atgataaaag tgggttatca gatatacaaa aggatcggtt aaaggcatta caataccgat
    16381 tggaactaga tgataatggg aaagaaattc ctgttcaaat tagtgacgaa ggcttagcaa
    16441 aatatgtcta tacttcaggg tatcgtcgat acagtagtaa accaggtatt attttagctg
    16501 gtgaatcttc tttggcagta gcctatttat ttatcctatt tacgatcgtt acttgcatta
    16561 ttgagctggt atttaaaaaa ttctatttag tgattgccgc gtcaacggac tttgagacag
    16621 gtcaacggat gaaaagtgcg atagaagata tttctcaatc cttcttgtta ttggcgttta
    16681 cgattttaga attacgaatc tataccttaa tgctttcagg tatcggtgat ttgcatgcgg
    16741 caggaaagat caatggattc ttgtatgtcg ttgctttgat tgtactaacc attgcgctat
    16801 tcaaaggctc tcaatcagtt acgaaaattt tcggcgttga tacaagcctg aaaaacggca
    16861 gtaattctct tcttagcatg tttgccttag gaaatattgc gaaaaatgtg ggtagcggag
    16921 ctaaaaacct tgcaagtgct ggtaagagtg gtttaagcca tttaaatgat attcgtaaaa
    16981 atggatttgg ccatcgaaaa gatgttaccc ctgataacgg ggaagaacaa tcagacgata
    17041 aagctacaac tgtacctaat agtaagaaaa cagtttccga aatgttcggg aaagcgaaaa
    17101 atggtgtttc taaagccgca gaaggtcttg gttacgtcaa tgaacgtggg ttaaaaggaa
    17161 cagctgaaga tcttacagaa aaagccaaag acaccttaga agataaatta gacggcacga
    17221 gagctaaaga attggctgaa gctgtgaaga atccatccga agcactagaa aaacaaaaag
    17281 aaaaagcttc agaagtcaaa gaaggactaa aagatgcgta tggtgaagga caagtcaatg
    17341 cagcagtgaa agctaataca acaaccgaaa aagaaaatca agaaggtata gtgattcctt
    17401 cagtcaaaga tcaccaagaa gcgttgcact ctaatgaaaa ttcgattgaa gagaaaaatt
    17461 ccatagaaga aagaaattca gaagaaggaa aaaatggaca gttaccaaaa actgatccag
    17521 aacaatatcg ctataaacct aacagtgacc ttgaaaatga gaagcgaatt tcagatttat
    17581 caaaagtaaa tcaagaagac aaagcagaaa atgagggaca gttaccaaaa actgatccag
    17641 aacaatatcg gtatagatct gttaactccg aaaatgaaaa acgcatttca gatttaccaa
    17701 aacgaaatca agaagtacaa acggatgagg attccatcgt tctgccaaat gataatggaa
    17761 tcgaagttgg aactgaaaaa tcaacgaaaa ttggtttacc taaggcacaa aatttaccaa
    17821 tgaaagatag tcgtttgaat caattcaagg ataatgatca aagttttgtt catcaatcac
    17881 ctgcaggtac tgttcaaatg aaaccaagtc gtttgcctga aaatggaaat ctgacaactg
    17941 tttcttacaa tttatctgaa agtggtgaga cagggaatgc gtccgttcaa aatgttgtga
    18001 caagagaagc tgcaccaaaa cctattcaaa acgttcatac aggttacaag ccagcaaatg
    18061 tggcatttac tgcaccaaca aaaataaatg tgacaagaga aacaaaaaac aggaacaatg
    18121 cattgaaaga acaactattg agggatatca aacaggatat caaaccataa aaggcctaca
    18181 agttaggcct tttttctatg tatgaaaagg agaaatcatt tatggataaa tatagtaaca
    18241 tgaaaaaccc tccaagattt agtggagaat ggaagttttt cgcttttttc tttttagatc
    18301 tattaacgct tggtggctgc tttttttggg cgaaaatggt caataatctt gtccacttac
    18361 cactcttttt tgcactcttg aattatggct tatggattgc gatagcgtta gtgattgttt
    18421 ggcgacccta taataaccca ggtcaacgac aaatttttgc gattatcgag tctatttttt
    18481 ttgcggacaa gaaccattat catgcgttag atcctaaccg aaattatcat ggtacgtata
    18541 aggaggatga ataaatgcga gtcaaaggac aaaattcagt tgaaaggaaa tccattttgc
    18601 agtttggaaa accagaagag cgctctatct tggacttatt tccatataaa tcctgtacag
    18661 aagagggagt cttaattact agagaagatc attttcaacg ttattatcgt gtagtttcta
    18721 cagacgtgga aggattaaat gaacaagaga aactagaacg aatgaatcag ttgaccaccg
    18781 tcatgcgtac gtatgtgcca acaattaagt tagttagttt gaccacagag acggatctaa
    18841 cggaacaaat cgtgcaaaag cggcaattat tagataaaaa tcgtatggaa cagtccttag
    18901 gaagaaatct acaacgatta aaaaaatatg aaaagaaatt agtcgaagaa atccaggaat
    18961 tgaaacgagc agaacaagaa cgtcctgact taagcttctt tttcgtcatt gaaggaaaga
    19021 caatcaagga tttacaaacc agaaaccgtc agttaatgcg atctagtggc attttagggt
    19081 taaaaccact ctacaaaaaa gaattgatca agattctgta tcgaatgaac aatatgaatg
    19141 atgagtagga atggagaaga gagaatgaaa tatccgttga tatggttaga aaaagatgtt
    19201 ccgaaggaag agaccactag aaaatttcct cacactttaa gcctgggtaa gttgggaaaa
    19261 gggcaatctt ttaaccaagt aaagaatcaa acaagaagag gtgactgtac atgttagaag
    19321 caattaaaac agctttttca ccacaaaatg atcaaaaagt acttgatcca ttaaagcaac
    19381 taggaaatcg ggaaatagaa attgtaaaat ccaaagggta tgatttagat tttttaaaac
    19441 aagtgatgcc acaaggtggg ttaaattttg atgaagaata tgtgacgtat ggtaatggtt
    19501 attcacgatg tgtgacagtt tatcactaca caaaaaatcc tacgcttttt tggttggcca
    19561 atctcatgaa caatcctcat acagtggcca cactcgatgt tagcacagat gaaaagcaaa
    19621 aagtgattga tcaaatcaat cgtgccttag atgaattaaa tgatcgatca gataatgaac
    19681 gaaaagcgac tgaccgaaat aaaagcgcgg aagaatacct ggaactagcg gaatatgcta
    19741 gaaaattgac ttctaatggg gaaatttcta agcaagtcaa agtgagagta catgtttacg
    19801 gaaacacgat tcaagaagta gatgaacgag cgcaggaact gatcgaaaat ctcaaaggga
    19861 tggattatcg tgcggtgact tatctgcata aaaccaaacg agattggcaa tcgatgttta
    19921 ccatgttcga tgaacaagct gaatggttcg gaaatatcaa agggcaatca ctaccatcgt
    19981 taaacattgg cggaggtttc ccgttccacc accaaacgct aaaagatcca ggagggacac
    20041 aatatgggga aaccatgaca ggcggcccat ttgtctggaa tgcgacgtat aaaaatagtg
    20101 tacggttgtc tactaatacc ttgcttctag gaatgatggg agctggtaaa tcgaccgttc
    20161 ttaaaatgat cgcggaagcc catttagctg caggagactt tatttggggc tttgaaaagg
    20221 gaaaagactt tattcctttc ttaaaggaat ataacggtat tatggtgcgt ttagacggat
    20281 ctgacggaat gatcaatcct ttagagatat ttgccacacg gacgtatgat gaagcatcca
    20341 gtttatatga cgatggatca gtcaaagatt tgaagatcaa tgaagcagcg tcctatcaaa
    20401 cacacttaga caaagtggtc tatcaggtac agttagtgag tcctcagcta aaaggaacga
    20461 tgaaagcaga atttaagacc tacttaaatc gtttttatga agagtatgga acagtaccac
    20521 gcggctttac ttcctcaaat tcgcgttcaa atactgatac gcaagtcaca gggaaagatc
    20581 cagaagctta tccgacgttc aaagaatttt tagacttttt aggacaactg gaattaccag
    20641 gtgcttccca agagaaaaag aatcgaaaag aagaaatgga atccatcgta gaatccttat
    20701 gtgagacgta tggcatgatt tttgatggtc atagtacgat tcgacacctt gaccaacaac
    20761 agttggtctt ttttgacatt gactctatta caggcttaga tcgaggggta caacaatgtc
    20821 aaatgtacat ggctatgacg ttgatttgga atcaggcatt gattcaagga agaagacaac
    20881 gacgtttaat taaagaaaaa caaatggaag aacaagactt caaacgcttc ttggcgatga
    20941 ttgatgagtg ccataatggt gcgacctgtt ctgcagtgaa aagctgtggg ttgcaagtag
    21001 tctaaaaact attgtaataa ctcttataac gagaagtcaa atgagtgggt aacgccacaa
    21061 agggcttcca ctgatactcc gacatgcaac aagtagttta tgcagaccaa cgttgtgtga
    21121 agctcggtga agtcggctga aggataccgt aaggtaaaag aaatagtgaa taccacgaaa
    21181 gttctctaga ggtaaagggt gtatccgata agtcgggtgt ccaaaaaaac ttaatatggt
    21241 gagtatgtac aaacgtactg acgaactagc gaatgtactg tccgaaaggc atagctccta
    21301 gaaatgggag agacagtgag caaaaaatca ctcattgtgc taacttgtgg ttgagtaaaa
    21361 gtcgttgtta tgaaagacca tgctgcgtta caggcgtagg agaagttagg gggcaaaagc
    21421 tagcatctaa gttaaggcat gtggatagat ataagggaac aggggaagcc atcaacatgg
    21481 agagtgcata ctcgatgaag tggtagtaag aaatatgtga atgacaaata acttacttta
    21541 gtgatggtga aagtagagtc acgactgatg aaggttctgt aatggaactg gaggaacagg
    21601 ctcgattcgt ctgtaaaagt catgaaaccc gttcaaccat gggattctcg tatgactaaa
    21661 aaggtcacga gccaattgga gcgtgataaa ccatgacaaa agaaacaagt ccaaaagata
    21721 ggaagttgcg gcatgcagaa tattatggca tgacggaaat cttcgataag atatactcca
    21781 aaagtttgaa gggagataat ttcaagaatc taatgcagat tatcatttct gataacaaca
    21841 ttgtcctagc atatcgtaat atcaagagaa atcacggtag tgtgacaaga ggagaagatg
    21901 gagtaacgat taaagatatt gaaaatctca accgtaatga attcgtagaa attgttagga
    21961 agcgattcgc acattataac cctcggaaag taaggcgtgt agaaattcct aaatataatg
    22021 gtaaattaag accgttgggt attccatcaa tatgggatag aatcgcgcag caatgcatct
    22081 tacaagtatt agaaccaata tgtgaagcaa aattcaataa tcacagctat ggtttccgcc
    22141 caaatagatc agcagaacac gcaatagcag attgtatggc ccgaatgaat caaagtcaca
    22201 tggaatatgt ggtagatgta gatatcaaag gtttcttcga cgaagtcaat catactaaac
    22261 tcatgagaca aatctggtct atgggtatac gagacaaaca gcttctctta atcattagga
    22321 aaatgttaag agcacccgta gtacttccaa atgggaagtt gatataccca acaaaaggaa
    22381 caccacaagg cggaatactt agcccattac ttgcgaacat caatctcaac gaatttgact
    22441 ggtggatagc aaatcagtgg gaggaaaaag atgccaaaga gctaaaacat cattttagga
    22501 aaaatggagc ttgggataaa ggtaatatct atcacaagct cagaaagagt acaactctaa
    22561 aagaaatata cctagtgaga tacgcagatg attttaaaat tttctgtaga aattataatg
    22621 aagcgaaaaa agttttctat gcaactaaaa tgtggttaca ggagcgtcta aagttaccaa
    22681 tatctgaaga aaagtcaaaa atcacaaatc tttcaaaaga agaaagtgaa tttttaggct
    22741 ttacgatgaa attagtgacc aaaggtaaca aaaaggttgt taatacgcat ataggtacaa
    22801 aagcacaaag catgattaaa gtgaaactaa aaaaacaaat caaacgaatt caaaaaagtc
    22861 ctaatagcaa agataccttt atctatattg gagtctataa cagtatggtt attgggattc
    22921 ataattactt caaaattgcg actcattgta gtgtagcaat gcgaaaaatt gcaagagaac
    22981 tcagaccaag ttttgaaata cgactacgag aacaaggaat ctcaaaagaa ggacaatacg
    23041 gaaagtacga aggatacaag ccttacctac ggtcaggaca aatacgatat ataagagggt
    23101 ttccaatatt accagtaggg tatattcaac acagacatcc gatgtgtaaa aaaagaaata
    23161 ttaataaata caccgaagaa ggtagggaag ccattcataa gaagcaacaa accgtagaag
    23221 aatggaagat atcgtggttg tgtagcaatc cagtgcttag taagagagca acgattgaat
    23281 ttgcggacaa tcgaatttca aaattcatcg ctcaaaaggg aagatgtgca gtaacaggtg
    23341 aggagcttat cttatctgaa atgcattgtc atcatattat cccttatcat gaaagcaaaa
    23401 gtgatagcta tgaaaaccta gtgattgtta ctgaggaggt tcatcgggta atacacgcta
    23461 cacaaagtga aacgattgag gaacttctaa aatatctaaa attaaatcca aaacaaaaag
    23521 aaaaactcaa tgagttgaga ttaaaagttg gtaacgaaga aatatcttaa taaatatttg
    23581 taggacggga aaacagaatg tcttttatag acgagggaac gccgtatgcg gtgaaagtcg
    23641 cacgtacggt gtggactggg ggaaaagacg gagataatat caaactctta cctatcagta
    23701 tcgattaatc cagattttat tgaaacggta aattatgtca caaactttca acgagaaatg
    23761 cgtaaagtag aagcaatgac tgttttagct acacaatccc cacaagaaat ggtgccagaa
    23821 aatatgtctt ctgataactt ctcgaaactg aagaaagtct ttgaattttc ttctacgaag
    23881 atctttatgc gtatggatga atctatttta cgtcatatag aaggattagt gggtaaatca
    23941 atgacgaact cagaattaga atccattcct caacaaaatc aaggagacgc tgtgatcaat
    24001 tttggctcaa aagaaaccat tcgtgttcat tttagtccga acaaacgtca attaaaattg
    24061 tttgatggag gaaaataatg gaaaaccaaa atgaaagttt aatcaagcag tatgttaagc
    24121 gtcgtgcaaa gcggcgctta tttttgtggt tgtttggtac ttctgcaggt ttgattacta
    24181 ttttgatcac tgtgttcgtg accctatttc taatacttgc ggcgggctcg attgataatt
    24241 cggattcaga ttcaagcagt ggtggcgaag cttttacagg agaatattcg gaaggcttac
    24301 cgatttataa agaaatcaaa ggaagaggcc ctttttctga tgagattgcg caatatgccg
    24361 taggtgcagc agtgaaatat aaattattac caagtgtgat cctttcgcaa tacggttacg
    24421 aatcagcgtt tggtacatcg gcgtccgcaa gaaatgatct caactatttt gggattactt
    24481 ggtttgatgg ttgtctgttt cctaaaggaa ctgcacgagg aattggtggc attgaaggtg
    24541 gctggtacat gaagtttccc aactcaaaag ctgcattttc ctattacggc tttatggtag
    24601 ccacacaatc caattttaat gcttgtgtag gaaataaaag ccctggcgca agcttattga
    24661 tcttaggacg aggcggttat gccgctgcag gaattacgga agatagtccg tattatacag
    24721 gttgtatgtc aattatcact tctaataaat taaccgaata tgatgaattc gccatcaaac
    24781 attggggtga aggtggcaat aacaacggca cgattactgg tgaatggacg aatccattcc
    24841 ctggcagcag tttagataaa agttccttca gcggtggtca attatttgga acgaatccag
    24901 gcggtgaatt ccgtccgaac ggcttccatg atggattaga ttttggttca gttgatcatc
    24961 caggaagtga gattcatgcg gttcatggcg gaaaagtcgt gtacgttggc aatcctggta
    25021 tttcaggttt gggcgcgtgt gtgatcgtga ttaattacga tggcttgaat atggtgtatc
    25081 aagaatttgc aaatagtaca ggaaattctc gagtgaaagt aggcgatcaa gtgaaagttg
    25141 gtcaagtaat tgggatacga gatacagcac acttgcattt aggatttact cgaatggatt
    25201 ggcgtcaagc acaaggacat gcattcattg atgatggtac atggatcgat ccgttaccat
    25261 ttttgaatag cagtaaaaaa tagatcagga gaaaaaatat ggaaaataaa acgttaaaaa
    25321 tcgtcttaac agctgcaatt agcgttagtt taatcggcaa tattgcgttg ttttggcagc
    25381 tgaaagagga acaagcagca aatacctata aaattgaaga agtggctaaa aacgcgtctg
    25441 agaagctcaa aaaaaattcc tctgaggcac aggcgaagat cgacaagtta aatcaaacca
    25501 tcaatgagaa aaaccaagaa atcgatcagc tgaagcaaca agatggcaca aaagcaaaag
    25561 aggaagtaac tgaagcacaa agaaagacgg cagaagagtt cggtcaattg gccattgata
    25621 aatcattaag cagaaatgaa ttaacggaaa aactaaaaga tgtcgcaacc caagaagtga
    25681 tcgacaaatt aagtcctaca gacgatgatc accaacatga tgattacgga agttactcct
    25741 ttacattagg tgacgtgcaa acttacgctc aaacaagcag tgtaaaagag gaacaaaatt
    25801 ttgtcgtatt tgttgattat acaatcggca atccgcaatt caaagatgtc aaaccacaaa
    25861 aaataaaagg tggattaact gtcactgaaa aacaagtaga tggtcaatgg aaagtcatag
    25921 actttagtta tttcactcgt taggaggaag aaaatgagta agaaaaaagg agaaagacaa
    25981 cgtttcaaaa aacgaaaaag ttatcttaga tggctgggaa tctttatgat tctcggcttt
    26041 ttgattcttg ctagtccgat ttatacgaat cagaaggtag atataccaga tgaggtaagc
    26101 acgaacgcct ttttaaacac gaaagagctg acaatggttt caaaagaaaa aggagcgaac
    26161 cagcaattag tttttgaatt tgcgatcgat gatgaagatc aaagcttgtt aaaagaactg
    26221 gctaatctta attatcgcgt agaagtaaag acagctaaag gggattatca agcaatacga
    26281 gccaaagtga ttaaggtgtc tgatgattat tttgcagtga agctaacgca tgtaccagaa
    26341 gaatatatcg ccatgcgttt aaccattatt cctgaaaaaa ttgatccaaa agtggatatg
    26401 caagaaccac aagatctgat ttattacgtg cacgaagata aggttaaaga tcaagtgaaa
    26461 gatgaagatt atgaacaaca cgcgatcaat tacaaagtga aaggatacaa gaaagaacaa
    26521 aaagcagctc aaaaacaaat ggaatctttc caagcaacca ttgatttgaa tgaagaatta
    26581 gtgaagaagc taaaagatca attgccttat caagtcgcag atgatcagga aaatactgaa
    26641 aacaagatta atagctacca gcaagaaatt gaaacatcga aaacgcaact gaaagatcaa
    26701 gaagagatca ttcaaaaact tcaagaaaaa attgatcgta tgacaaaaga aaagatttag
    26761 aaggacaggt ggagtgaaaa aatgaagcga acaaacttgg ctgacaaaga tactttcgct
    26821 gagcagaaag tattgattaa agcctatctc aaagttgtaa aagctaaaga aatatgtcga
    26881 gaagtgaatt tatctaacac aacaatatct gagttgaaga atggtcgaaa gaatttagat
    26941 actttaaaat tcagatcatt tgaagctcta tatgtagctg ctaaaaaata ttttacagct
    27001 gataacaaat aaaaaacagg agtattttat gtgaaaaaag tatataaaaa atgcttatga
    27061 atcagctata tcaccttaac catttgttaa tgagatggga gaaaacatta accacgattc
    27121 taacgcaaac atcaagcaca taaaacattt aacactcctc ctttctggct gaagatttca
    27181 accaaacagg aagacagcgt atcaattata gcatacactt tttcttaaat tcatttttca
    27241 tatgacaaaa tatttccttc taatcgacaa agttagaagg attttttatt gtctaaatac
    27301 acaaataacc ttaattttta aagagcataa ggaaacgtga tatcactttc tcacgaaagg
    27361 tgttaggtag gctgtgccta ccacgtttct aagaaagata aatgatatca aaattttgga
    27421 tttgtgtgta gatttgtggg taggatgaaa gcgaatgtgg gcagtaaaat aagcgtttgt
    27481 gtgtagcaaa atcaaaaaat gtgtagggat atgtgtagct acacatatcc ctacacataa
    27541 taaatttgga ggaaaatatg gacttattaa ttagaaatat tgaagaaaag tatattaata
    27601 aaattgacaa acgttgtgaa gaacttacgg tcaagactgg caaaaaatgg agtcgaaatc
    27661 aatacctaaa agttttgatc gaaaatgatt ttgatcacgc tcttctgaat tataaaaaag
    27721 accaatttga tcggttgtta gaaaaatttg tggatattca aacttataat acgaaaactt
    27781 tagaagaata tattgcaaca aataatcaat taattggttt gttaattgaa taataaaaat
    27841 agtattgtga atttataaaa ggaggaaatc aattttggca aaagcaacaa gaaaacatgt
    27901 atatctttac gatatgcata tcgaagtact tgatgaaata atttctactc atgaagatat
    27961 atctaattat agtgaagcag tcagacattt gtgtatgatg tatgatccaa aaatcgaaga
    28021 aaaaataaat ccaattcaaa ttaaattgaa tgcaatggga aaagaaattt ctattttaac
    28081 cgaaatggtt tcagagtttg cggatcttca tttgaaagat acaggaatac taattggtac
    28141 aaattcaact gtttatcaag aagcagaaga acgtgtggaa ggaaaaatta aatccaatca
    28201 aaccaaaaaa tattcttcta agttaaagaa accagtgaat gaatctgttc ataccagagt
    28261 agatccattt gatccaacga agattctaaa aagataaaag tagaaggaga acgtgatgaa
    28321 taaatcgcct gcagttattc ttacttgtca atttaccttg cccaataaaa aatctttttc
    28381 aacatacatt gattatatga caagagaaaa agcactggaa gaaatggaac gacgtacgcc
    28441 cgaagaagaa caggaactgc aacgaatcaa gaatgcttta gaggattttt atattcctga
    28501 aggagaaact ttttcggaaa gtaagacgga taaagaattc agtgaaacgt cgaatgaagc
    28561 tcaatcgttg atggaacatg gaatcaattt tgacaagtta gaggaacaag attttaccaa
    28621 atatctttct tatatgaccc gtcactatgc gttagaacaa aagaaagaac tgacgcaaag
    28681 ggaacaaaaa gaataccaac ggttgaacca agcaatgaaa aaatatcaaa cagagccaca
    28741 taaaaaagct tcaggaaatc ttcctggagt tttttctatg gcttcagacg aagtgaaagg
    28801 aagtgatctg aaggagatca aacaaatctt ccaaagagga caacaaaaag gctcaatcat
    28861 ttatcaagat gttatctctc acgacaattc gtatcttgaa aaaatgggct tgtacgatcc
    28921 caaaacagat accttagatg aagaaggttt gaaagcagct ggaagaaaaa tgatggagac
    28981 attattcgag aaagaacaat tgaatgaaac aggttactgg atggcaacga ttcaccgtaa
    29041 cactaagcat atccatattc attttgcagt tgttgaaaaa gaaaatacgc gtaaaccgta
    29101 tactgagatt gaaaatggtg tgagttatat cgtaccgaag gggaagagaa gacaagcaac
    29161 attagacgca atgaaaacca attatgtaca tgagttagaa cgtttctctt atgaaaagga
    29221 tcgaatgatc ggtatggaga aacgaaattt attgacacga aaaagtgatt tgcgtaacac
    29281 cttaacaaaa gtagtaaaag aaccagcacg ttacgatcga cacgccatga acttattaaa
    29341 tgaagtatat aaaaccctac ctgaaaaacg ttcggaatgg aattatggag atgaaaatcg
    29401 aaccaagctt tcaccattta caaggggaaa acttgatgag ttaaccaaat atgttttaac
    29461 aaaagaacct ggatatcaag aatatataca gatttcaaaa gcattgaaag aagaagccaa
    29521 aagcctttat ggggaaagca aaagagaaac aaagaatgca gaaaaaaacg ccctctttga
    29581 tttaaaaaag cgcacaggaa atgcgatttt aacagaactg aagagacagg atagtcaaat
    29641 aaaaccatta atagaaagtg tcaatcagat gcaatctatt tacaatgaac gatcctttcg
    29701 aacactcaaa cagttgccaa caaatgaata caagtatagt tatcaagaat tcgtaaagcg
    29761 taggaaacaa ttccaacaaa gagttgtcac gcagcgaagc ttgcgtcaat taaatcgaac
    29821 aattaatgca catgaagaaa aataccacgc aagtaaagca tatgaagagc aacagcgaag
    29881 aatccaaatg gaacaagaaa gacaatagga ggaagagaaa tgaaacaata tttagatcaa
    29941 tggaaagtca tagaaggatc actaaaagaa gaacgaatcg aacaacttcc tgattgttta
    30001 gaaaaagagc atttgtttca gataagcgaa atgttgagaa acgaacagtt tgatccaaat
    30061 caatttttag ttgttgagta cccagctact ggtgtgtatt gttgtaatca tgtgaagggt
    30121 gaaaaatatt ttattattca agaatacgaa ggtaagttag cgccttatta tactacttgg
    30181 gaaataaatg aagagggaat caataatttc ccatgtgaaa gcattgaaga gtctatttct
    30241 ctcacggaat gttaaataaa tagagacgta aaagagtcct gaacgtgatt gtccaggact
    30301 ctttttcaat gaattgtttt ccgccaattc actgagtggt gcgattcaat tttattatat
    30361 caaaagaaag tgagattcca aagtgaaggg aggaagaaga aatgactcag acaacgattg
    30421 gtgttgtggt tgaaaaaagc agggataatc tcgtttttgt gacagaaatt caaacaggta
    30481 gagcttttgt agtcacagac aaagctgcaa aagcgtatca aaatggcaat attttaacgt
    30541 taaatctgac tacgaagatg ttcgttgatg cagtagaaaa ttatccattt gtttagacaa
    30601 acgatatttt acaaggagga aacggacatg gcagaagaaa aagaaccact agataatagt
    30661 cggttgggaa agtccaaaag aaaattagta cggttgcaaa acgaattaaa taatcaattt
    30721 caaaagctat ttgatcacca aaaattgaca aatgggcaac caatgaatga caaacgaaat
    30781 ggtcgttcat ggtttaacca gcaagaaaaa atagaaaata aaatacaaag tttgagagaa
    30841 gagatcaagc aacaagaaaa acaggtagaa aaacttgaaa gacaagaaga gctaaaggaa
    30901 atgggctata acaagtacgg cggactggat atgacgatcg ataatattcc aagaatcaaa
    30961 gaagaaatcg aacgctttga aaaaggtgaa tcgacttttt cagccgccac gatccgaaaa
    31021 tatcaacgga agttggaaac attagaacag ctgaaagaaa aatcagaaaa aggaaaagaa
    31081 aatatcctcc ctgaagtaca agcgatcatt gacagcggac gagtgacgca atggaaaaaa
    31141 aatccaacca tttatttttt gaaaggctat cgaaaagttg ctttagaact gaatgaaaac
    31201 gggggatttg aggaatcaaa aaaataccaa gccacgtcgg aagaggaaaa agcagctgtt
    31261 cgtgaattac ttgaagaggc tgcaccaact gattctctga aggagaaaga aaaagatcat
    31321 ttctatcgag tagaattcaa cgaaaatgat ccagaaaaag gattgaaaag ttatggtggg
    31381 gagatcgtaa cattagaatt gatcgatcaa cttaaaacgt tggataacca atacgctgca
    31441 gaagcaggtt attataaatt ctattttgaa gaagtgaaga acggagaagt agtagatcag
    31501 atccgaatgg atctagggga tggcgtcaac agtaatcacc ccatctattt agagttagca
    31561 aaattgtgcg aacaagtacc tgagaaggaa caaaaacaat cggtcaaaaa tgaacctgtt
    31621 tcgaaagaac aaatgcagtc gaaagaaaca aaaacaaaac aaaagggcta tcaaaaacga
    31681 acggcagaag agatcaaaca agaagttaaa tccctttcgc aacaggcact ggatgcggtg
    31741 aaaaagcata cccattcttc tgaagacgtc aaagaacttt tgaactttat gtctcgtttt
    31801 ccagaacgat cctttagaaa tcaattattg attgaggaac aatttccagg tgcaacggct
    31861 tgtttaggtc gtgccgcgtt gaaaaaagaa ggaatctata tcaaaaaagg ggaacaaggg
    31921 aacaagatct ttgtcagaaa aacggtcaaa ggattttatg agaaaggacg tggctttgtt
    31981 cgagaaacag aagctacacc agaagataaa aaacgaatcg aaagtggtca aatcgaagtg
    32041 atcaaaaaac cttactacac gattgaaaaa gtgtttgata tcacacaaac acagttaaaa
    32101 cctgaagact atccgaaaat ctttccgaac cgtgtctttg attttcaatt ggataaaaaa
    32161 ggacagtcag aacttcaagc tgggattcaa gcggtagcca acaatattgg aatacaaatt
    32221 cgagatatga cagaaagtga agtctatcaa agagaattag gacaagcacg tggggcgtat
    32281 gttcgtaacg aatttactgg cgaagaagaa atcgtcatga atactcgaaa tactcctacg
    32341 cagcatttag ctacaagtat tcatgaatta gcccatgcaa gaatgcacaa attttcagaa
    32401 ttagataccg caactaaaga attgcaggca gaaatgactt cttatattgt gtgcaaacat
    32461 tttggaatgg acacgtcgga gaaagctatt ccttatattg cagcatggac gaagaatggt
    32521 cagacgttgg atgataagga agcggcggaa cgtgggaaaa tcatgaatga tgtcagccgt
    32581 gtcgcaaatg aattcattca aaccatttct aatgaaatca atcaatacag agaaagagaa
    32641 ccagagatcc aaccagcaga agtgaagcag gagcataaaa atactgtaga aacggcagag
    32701 aataagaata ttgtgcccgt tggttcactt tggatcgaca agaaagacgg gaaagtaatg
    32761 gaagaagata cgggtcgaac gttacatttg aaggatgcgg cgtttattga agatccgcag
    32821 cataatcaag tgattttaaa aggaaaaagt tatggaaaag agattcaaac ggcgttacct
    32881 tcagaagaat ttgcgctagg aaatccattc cgattttcac ttgaaacagt gtcagaagtg
    32941 gccaaacgaa aaaatgaaat ctattatgaa caacaattgt cacaaaaaga acgcgtataa
    33001 aacaagtaaa aaagactatc tgaaaaacgg atagtctttt ttcttttggg aggtttgaaa
    33061 atgaccgaaa aaaaaatgag tcttattgat cgatgtaaac agatcgatat agtggatttt
    33121 gctcgaaaca atggaatggc tgtagtcaat aaaggacgag actatcgctt agaagaccat
    33181 gattcatttg tttttgatcg aagaaaacaa cgtttttatt ggaacagtca aaatattagt
    33241 ggcgacatta ttgaattagc taaacttttt ttcatagaca aagagatcca agactctaag
    33301 caacaattta aagcagccac tgattttatt ctaaagaatg aagacaaaac agaacgagtg
    33361 gaaaatcttc attttgaaac agaaaagtac aaagatcacc ccgttgatta tcaaccactc
    33421 acagaaaaag gaaggaatta tctgaaggaa gaacgaaaac taccagagtg gctgattgat
    33481 tatgcagaaa aagaagggtt gattgccgaa ctaaagccaa aatatgaacg gcaaaatttc
    33541 ttagttcgag acgatcgttt agatcatgcg gtcgcctttt tatggaaaga tccacagaca
    33601 aaagagacag taggggcgag ttatcaagga acgttcattg attatgaacg ttttggtgag
    33661 agaggcacat ataagcatat agataaaaat tcgacagcga atcatggctt taacctaaag
    33721 atcggtgacc ctaagcagtt aaaatttttt gagagtagta ttgatttact tagctatgct
    33781 gcactgaatc gtgatcagtt aaacgatacg tggttagtct ctatggaagg gttaaaacac
    33841 catgtgatca gtcattattt tggtgaggca gtatctgaat tgagaaagaa acaagccttt
    33901 cctcaatcca tcgagatttg tgtggataat gaccgtgcag gacatatttt ctacgaaaaa
    33961 gaacaattaa tgggcgcagt cgatccattt acgaatcaga aagtacgttg tgaacgagga
    34021 atcgctaatg attggcaagt gccaaaagaa tacaaaatca tttatgaaga agtcgcaaaa
    34081 gaagaaaagg tcacaccaga agctattatg gccatacaca agacagaaaa caatctgcaa
    34141 ttaacgaacc aactggtttc cgctcacaaa gtaaatgcgt tttttggcca gcagctttct
    34201 gttaacgatt caatagaagc cattaatttg aaagatattt gtagagaagt cgcaaaagaa
    34261 ttaaaagttt gtgaacgcgt ggatggtacg tacgattttg atcgttttta tcaagaaaaa
    34321 ggggatatta atgcgcagat tcttttctct tataaagcag aacaatacta caaaggctat
    34381 aaaaatcatg aacatgaatt tatacctgaa gtaaaaaagg actggaacga tcaattgaag
    34441 catgagatcc agcaacaaga aatcagaaag caaaaacgtg ccatgttgtt tcaacaaggc
    34501 agacaacaag aaagggagtg aaatcgtcat gatgaaaacc gttattttag cagaaaagcc
    34561 gtcacaagcg aaagcttatg ccgatagctt ctctaaagcc actcgaaaag acgggtattt
    34621 tgaaatccaa gatcgattgt ttcctggtga aactgtgatc acgtatggtt ttggtcattt
    34681 agtcgaatta gattcgcccg atatgtacga tgaaaattgg aaacaatggt cattagaaca
    34741 tttgcctatt ttccctacac attatcacta tcatgttcct aaagataaga aaaagcaatt
    34801 caaggtggtg aaacagcaac ttcaatcggc cgacaccatt attattgcga cagatagcga
    34861 tcgtgaaggg gaactgatcg cttggacgat cattcaacaa gtaggcgccg atcaaggaaa
    34921 aacctttaaa agactttgga tcaactcctt agaaaaagaa gccatttatc agggattcca
    34981 gcagttacga gacgcaaaag agacctatcc aaaatttgaa gaagcacaag cacgtcagat
    35041 cgccgattgg ttaattggga tgaacggtag cccactgtat agtttactct tgcagcaaaa
    35101 aggcatacca ggaagctttt ccttaggcag agtccaaacc ccaacgcttt acatgattta
    35161 tcaattgcag gaaaagatta gaaatttcaa gaaagaacct tattttgaag gaaaatccga
    35221 agtaatcgct caaaatggcg catttgatgc gaagcttgat ccgaatgaaa cccaagcgac
    35281 gcaagaagca tttgaagatt atttaaaaga aaagggggtt caattgggaa aacagccagg
    35341 catgattcgc caagttgaaa ccgagaaaaa aagcgcggca agtccacgtc ttttttctct
    35401 atccagcctt cagtctaaaa tgaatcagct catgaaagct agtgccaaag acactttgga
    35461 agccatgcaa ggcttgtatg aagggaaata tctaagctat ccacgaacag acacccctta
    35521 tattacggaa ggggagtatg cgtatttgtt ggatcattta gatgaataca agcatttttt
    35581 aaaggccgaa tcaatcccaa cccctattca tacgccgaat agtcggtatg tgaataacaa
    35641 gaaagtccag gaacattacg cgatcattcc tacaaaaacg gttatgacag cagcggcttt
    35701 tgaacaacta agtccactgc agcaggccat ttatgaacaa gtgttaaaaa caacggtggc
    35761 catgtttgcg gaaaaatata cctatgaaga aacaacgatc ttgacgcaag tccagcagct
    35821 tcagttaaag gcaatcggaa aagtaccgat agatctaggt tggaagaagt tatttggcaa
    35881 agagaacgac ggaaaagaga aggaagaaga gccgctactt cctaaagtga caaaaggaga
    35941 aatggtgacg gtagatctac aagtccttga aaaagaaacc aagccaccac agccttatac
    36001 cgaaggtacg ttgatcacgg cgatgaaaac agctgggaaa accgttgata gtgaagaagc
    36061 gcaatccata ttgaaagaag tggaaggaat cggtacagaa gcgacacggg cgaatattat
    36121 tgaaaccttg aaacaaaagg aatatatcaa ggtagagaaa aataagctcg tggtgacgaa
    36181 taaagggatt ttgctttgcc aagccgtgga aaaagagccg ctgcttacga gtgccgaaat
    36241 gaccgccaaa tgggaaagct acttgttaaa gatcggtgaa cgaaaaggaa cacaaaccac
    36301 ttttctagcc aatatccaaa aatttgtctc tcacttattg gaagtcgtac cagggcaaat
    36361 ccaatccact gattttggat cgacgttaca agaagtgaaa gcagccagtg aaaagcaaga
    36421 agccgcgcgt catttaggaa tttgtccaaa atgccaggaa caagaagtct tactctatca
    36481 aaaggtggca gcatgtacgt ctgaagcctg tgatttcaaa ctatggacaa cgattgccaa
    36541 aaagaaactt acagcgacac agctgaaaga gatcattcaa aatagccgaa caagccaacc
    36601 agtgaaaggg ttaaaaggtc agaagggttc gtttgaagca acgattgttt taaaagaaga
    36661 cttcactaca ggctttgaat tttctgaaaa gaaaaaaacg aacttcaaga aaagaacacg
    36721 acgcactaca aaataatgga gggttaatct tatgcgtaat acaacacaca aacaacaaga
    36781 aatcgaaaat caaaaagcca aaattcgttc agaacaggaa aaaatgcgtg ccagcaaacg
    36841 aagactgcag caattgaaga accagcagcg gattcaaaaa gaaatcgaat tagatcgata
    36901 aattcctttc ttgttaatat aaaactaaca tgttatagtt gtgttagttt tatattaact
    36961 ttaggggaag agtaatgaaa gacaaaatcc aatgtatttt tgataaacat aatggcaacc
    37021 tatccttgaa ggatgctaga agtgaaggat tatcgcctat gacgttagat cgattagccg
    37081 aaaaggataa aatagaaaag gtagcaccag gtttctatac cttaaaggat gcgttcgtgg
    37141 atgaattgta tctcgctcag agtatctatt ccagagggat tttttctcat gagactgcgc
    37201 tcgatctttt tcaagtagga acatatatcc ctaaaaagat caatatgatg tttcctaaag
    37261 gatacaatgt atcaaaggag aaattagatc agtatgccat tcaaccgcat tacactaata
    37321 aagaaaattt tgacctggga gtcacagaaa cagaaagctt ttacggcaat aaaattttag
    37381 tctatgattt agagcgaacg ttatgtgaca tgtggaatcc aagatacaaa gcaagcgtag
    37441 aggtaaaaca agaagcatta aaagaatata tgacaagtac cagtagaaat acaaataagc
    37501 taagaagata catggatatt ttaaaatcac caaaagaaat gactttgtat atgttgccac
    37561 tttattgaga ggagtaaaga tgaatgacac cgacacaatt taaagagaaa acaagaaatt
    37621 tcgcagcaga gaaacattta aacccacaaa ttgtccaaag atattatatg atggaaaaat
    37681 tattagaatg tattgttgaa tcgccttatc atgatgattt tgtattaaaa gggggatttc
    37741 tgattggatc aaaatacggg ttagaaaacc gttcaaccgt tgatattgat acgacttatc
    37801 gaaattcgaa actaacaaaa gaaaagttag aaactgtttt ttctgaatta acagctaatc
    37861 ctacaaaaga tggaattcgt tttgaattaa agggattaac agagacgagg gaagccgatt
    37921 attatccagg ttttcaagca aagtttattg cttatcttga aaatgcacgt gtacctttta
    37981 aattagatat tacttcaggt gattctatta ttccagcagc attgatgtat gaacacaaat
    38041 taatgtttga agataaaacg attcatattc cagcttatcc tacggaacaa attctagctg
    38101 aaaaattaca tgccacgttt agttttggaa aagataattc gcgcatgaaa gatttttatg
    38161 acctgtatat gattccgaaa ttagaacgta tcgatgagaa aaatttgtat aagagtgttc
    38221 aaggaacatt caaggaaaga aataatcaat cggtgtttaa gcatttgtat gatcaggaaa
    38281 tgcctcaatt ggtgaaagat gagggcttga gagaaaagtg gaaaagatac caacaagaaa
    38341 actattttgc caaagacatt tctttcgaat ctacactaac ttccgttgat tccctgatgc
    38401 aatcaattgt agagcaagag aaggcagaac gaaaacaaaa ttttaagaga tcacaacaaa
    38461 tgaatcaaga aatggagaga taaagatgat caataatgta actttagtag ggcgattaac
    38521 taaagatcca gatttacgtt ataccgcaag tggcacagca gtggcgactt tttccctagc
    38581 cgttaatcgt aatttcacga atcaaaacgg caatcgagaa gctgatttta tcaattgcgt
    38641 gatttggcgc aaaccagcag aaacgatggc gacattagct aaaaaaggca gcttgatcgg
    38701 cgttgtcgga cgaattcaaa cacggactta tgacaaccag caaggacaac gtgtgtatgt
    38761 gacagaagtt gtggccgata acttccaact attagaaagt aaagcagcga cagaaagccg
    38821 tatgcacgct gatcaatcgt ccagcagtcc aagccctacc acctttgaac aacgggatac
    38881 agcgacgcct aacaacaatg gattgaacgc ttcacagaat ccatttggtg gacaatcaat
    38941 cgatattagc gatgatgatc taccgttcta agtagcattt tctattggtt gcatttgcta
    39001 cggtttatgt tataatttag acaacaaaaa gaattttcaa tctataacca aaaaccacta
    39061 gctggcgggc tagtggcttt cttttacgtt tctattataa gaattaacgg gtaggcttac
    39121 tgtctttctt ggcaaattta caatcaagcc atttgcagat atagtgcgcg gctacttctg
    39181 ccatgattgc aaaaacaaaa tcaaaaagaa ttttcaaacc tataacctcc tccccttgcc
    39241 agattaggcc ggggtagcag acagcaagac tattatagca taattgaaat atccaaatga
    39301 ttggattttt gctataattt tagcgaggtg attgtgatga acaaatatgt tggtttgctt
    39361 gataaaatcc gagtaataaa aacgcaaccg ttattggttc gctttacttt acaaacgatt
    39421 catgaatcga ttaattgtgt agttgcagat attgaaatta tcgataaatt gctaattatg
    39481 gatgatggaa aatacaatat tgccgtcaca ggtcacttca ataaacgaaa tcagctagtt
    39541 attgctagca tgtatgttag aaatcccgat cattttacgc gatcgatggg catataaata
    39601 aaagaagagt tgtagtccta aatgaggatt gcagctcttt ttgttttaca tagaaacagg
    39661 aaaagacctt tttaggaaga cacaagtgcc ttctcaattg aaaaaccttt ctctcactcc
    39721 aaccgccacc gccgttcgag aagaaaggaa gtgaacgctg cacgttcact aagatatggt
    39781 gtcttgcaaa gcaagacaaa atagacaaaa cttcctctca ccaaaaggtt cgttcctgtt
    39841 cgttatctct ttattgattt gccagaccgg caacaagttg ccttttatca ccaaaaagcg
    39901 acaaggacaa gggtaaatgg cacgctgcgc gcccttgtcc ttgtcgcttt tcgttgattt
    39961 attctggcca acaaatcaat aaagaaagaa ctaacgaaag gaagtaaaac ctttcctaag
    40021 aggaggcaga atcaaacatg cgaaagaaat ttgattattg gggtgttccc ttcagcgaat
    40081 tattcccaaa ctatcatgca ccacatactg ttgaatgcga ttgtggagaa agagcgaaat
    40141 gtatcaaaag ctatcgtttg tatcaatgtc caacatgcgg gaaaaaatat accttgtctt
    40201 atggcgatta cattttgatt gatgagaaag gaaagaaacg ctgatgaaaa cacaacacaa
    40261 attattgact gtaaaagaag tcgttcaaat tttggtggac aaagaagtca tttctgaaaa
    40321 agtagcagaa gctttactta ttccgcggta ctggacgata gaaacccctt tagtagagga
    40381 tgcaccgact atgctatttg aagacctagt gaagattgca ggatatgaac tgcattatca
    40441 agaaaacatg gaaacttatg aaatcgaata ttggttaacc aaattagaga actaaaaagg
    40501 agaaaaaata tggctcaaaa ttactatgac gaatttgtca aattaccttt ggataaaatg
    40561 gcacaaaaaa tggaggatat gacgttccta taccatgaaa cacgtgtacc caaaaaacat
    40621 tacaaggaaa aattgtcagt cgcagtggaa gaaatgattg aatcaggtgt cgaaatgaat
    40681 ctcattgcga cgtactatcg aacactggaa gaactgaaaa aacaaaatgg aaaatggttt
    40741 ttccaagcct tactatgctt ggaagtaggt gtcaaaccaa gcactatcaa accttccgaa
    40801 tatcaagcct tagaactgac ttatgcaaaa ttcgcacaga ttaagaaagc taaagcaatg
    40861 gctagcgact ggctagatac ttttgaaagt atcgaaaaaa atggtgcatt ttatacgatt
    40921 gaatcggagg agaaagataa tgaataaggt ggtttatcag ctaacaacag aagatgtttc
    40981 gcaattttta acaagcaaag aacacttgaa tgaagtaatc agacggattc aaaaagactt
    41041 tagttgtatg gaagaaatga agtactttat tcaaagagtg gaagaacagt tgtatatgga
    41101 aagcgaaaca agtcaaacga aggtcacagt cagccataat catttagacg tgtataaacg
    41161 tcgtcctgaa acggtaaaaa aaggaacaga gcttctacga ctagaaacag aaaaatacat
    41221 catgaatgtg tacctagaaa ggagtgagct ggtcgaaaac tttgtcagct acacgatttg
    41281 taggaaacac tattactcag aatcagaata ttattcaatt ttcgagagca tggaattaat
    41341 ggaagaagaa atggaagagc taaacagcaa aaatgcgacg gtagacgatt attaccttgt
    41401 atttactagg tttttgacgt gtgggacgga atctatcgtg ttaatgggaa tggattatga
    41461 actcgctgta cccgaagcat ttttgaggga atatgtagaa gaagtagata acccaggcga
    41521 cgactggtac acgactttaa ctgatttttt agacacctac acttacgacg atacagaaga
    41581 aatccaaaat gaacttgacc gtaaacgaat gccatatcaa cgatttgaaa ggaaaaaagg
    41641 taaataacca tttataccga aaagtgagga tgtgtgaaat aagaacaaaa aaccttgaaa
    41701 ttaagggaat ggttcttatt tcgcaccaat cgctccaacc gcccccgccg cttaggtggt
    41761 gcgaaataag cgggcaaagc ccgataacct aagagaaaga aggagatcca tatgaataaa
    41821 atcattcctc tattgttcgt actgtttgtt ggcgagatac ttatgcgtca tgctgcagct
    41881 gtacatttaa acttcgtagt attcttagga atactggtat tttgtttgat cttttggcga
    41941 tttgtattag cccttgtgag tctactattc aagatgattg cagccatatt tttcataggg
    42001 ttatttcgat gaaaggagag agtactaatg ccaattgcaa taggaaataa acggttaccc
    42061 gtgacattag atgaaaagag acaaaaagaa ttgcagcaac taaagcagaa gtacggaaaa
    42121 agtgaatcta agattatgtg tattgcgtta gatctattga tcgcgcaaga aaaagcagga
    42181 tttgatatac cagcactaaa aaagtgacgt cacttttcat ttgaagaaac aaaagtgata
    42241 gcacttaatt gttcctacta tttagaatat tttctatgtc ctttttattc cattttgtat
    42301 caataactat gaaatcttta aagtatccag aagagattat ttttagatca atttttttct
    42361 tacctgattc taaagatttt gttttcttaa acatattatg atttcctttc gactattttc
    42421 atataaatgt taacatagtt tatggattca tggaatgtta atctaattta atagaaaagg
    42481 ataattaagt atatacgttt tttcttatgt atgttatact atgtatatac agaaagaggt
    42541 gtctactatg gaaatgttac ctataaaaaa atggggaaat agtaatggcc ttagattgcc
    42601 aaagtatatt atggaatatc ttggaattca tacagaagac aaagtgaaga taatacaaga
    42661 agaagtaaac ggtcagaagc ggctaattat cgaagcggct aatatagaaa atgaattaac
    42721 aattgaacag ttatttgaaa attacactga tgaaagaaat catgtgacga ttcaaaattt
    42781 aggtgaagca gtaggtaatg aaaaatggtg agaaaaccta gacaaggcga tattctatta
    42841 ttgaatacag cacctcgatc tggacacgaa cagacaggta aaagacctta tattgtttta
    42901 agtcatgata ttattgcgga ttatagtaac gttgttacag ttgctccaat ttctagtaca
    42961 actagaaact atccgctata tgtaaatata aactcagaat atgaaatgaa gacaacagga
    43021 aaagtattat tagatcaact aacgacaatt gattatgaag ccagagagtg tatttttttg
    43081 gaaaaagcgc atgatagttt agttgaggag ttattgatga aagttagaac agtttttcaa
    43141 aaagtaagca aataaaatag aaacagcagc atgatcaact attcgtgatt gaccatgctg
    43201 tcttttttta ttctatatac ggtttgtctc gtaacattgt agtcttttgc aatcttagcg
    43261 atcgcaacac ctttttttag atcctcaacg atgtttttat aaacaagacg acgttgggga
    43321 tcttttgcat tggcactgta tagcttagga cgtcctttat aaacaccgtt cgcttttgca
    43381 atcttaatgc cttgtgcctg acgtcgttta gattcggtac gttcttgttc agcgatcatg
    43441 gccaaaattt gaatgatcaa atctttaatg aatttatcca ggagaggatt gccaattgct
    43501 tcggccatga tcggtagact tgtgatgatc agctgcacat ttttcttctt caagtaattg
    43561 actgaagcga tgatctcgtc gtaatttctt ccaagtcgat caatagcttc cacaataaaa
    43621 atatcttgat ctcggacgaa atccagtgct tcttgaaaaa caagtcgatg agtaatcgtt
    43681 gcgccagatt gtttctcaat gaaaattctc tctgcaccga attttttcat ttcttcaatc
    43741 tgccgttgtt cattttgatc gatggatgag acacgtatgt atgcaactct caaaattttt
    43801 acctactttc tgcaactatt taaaaaacac cctaatcata cacgagaaaa ccagattttt
    43861 caatgtcgcg gtcgtgtcta gttagggtat accttatata gacaaaaact aatgatggag
    43921 gaaatagtat gtgtcacttt tctttttaaa tggcaatatt ttttgtaaat aaatgtcaat
    43981 caagaaaatt taaacgatta tgacattaaa aatatatttt atgaattttc tagtatataa
    44041 cttgatttat ctgttaagat ttattaagaa aacgttttct tatttttgat ttcgaggtga
    44101 tagacaatga aaaataagtt tattttatct ttaggtattt taactattgg aattagtgta
    44161 actggaacta caattcctac atttgctgat agtacaaatg aaccaactac tgtagtagat
    44221 aataaccaag aattaactcc tttagatagg aacggaatag ccattggtaa cccaactaac
    44281 gacttaactg ttactgaaaa ttatttaata gtttctccat tacttgctcg agcagcttct
    44341 aaaacgatac gagtgacagg aacgatgatt tttgataaag ttggttggcc ttctagacct
    44401 cctcaatcac ggaattggaa agaaaaaaga aatggttatt ggtactatgg aaaattgtat
    44461 ttacaaagtt ataaatcagc tggaaacaat tggattgcga cttattcagg aaccttaact
    44521 agaaagtagg gattttttat gccagaattt gtgaacacac caaattttta tcaagatagc
    44581 gatattctaa gtaaaattaa tttaccgaaa gaagaatcag ctttacaagt tcagcgaact
    44641 ttacaggaga cacaaaaaat tcaaaaatca atttctaatc tagaagattc tgaagaaaaa
    44701 agaaaaaaaa gagaccaaaa aacatatcaa aaagaaaaat aatgatttta aaaaaagagt
    44761 gtctagtcga cattcttttt ttctaattga aagtgttcat aaaatcaccc atttctggac
    44821 actttctttt tttgctttat ttttcaaaaa aacaattcat ttcatgttca taaacccata
    44881 acaatatcta tgttcagtta aactatatgg ataaagttat gttacaataa aattaatgaa
    44941 ggagtgattt ttttgaaaat aggatacgcc cgggtatcta ctggtttaca aaatttggat
    45001 ttacaagaag acagcctaaa taaatttggt tgtgaaaaaa tttttactga tcatatgagt
    45061 ggggcaaaaa gtaatcgacc aggattagaa atggccattg attttgttcg ctctggcgat
    45121 acacttgtcg tttggcgctt agatcgttta ggtagaaata tggaagattt gatttcaatc
    45181 gtcaatcgac tgaatgagcg tggtgtgagt tttcatagta ttcaagaaaa tattacgatg
    45241 gacaaatcaa gttctacagg acagctcatg tttcatttgt ttgcggcatt tgcagaattt
    45301 gagcgtaatt tgatcctgga acgatccgca gcaggacgag cagctgctcg tgcaagagga
    45361 cggtttggtg gtcgtcctga aaaattgtct gaacaagatt tggaattact gaagacttta
    45421 gtggatagcg gtacacctat taagactatt gcggaacggt ggaacgtctc aagaaccacc
    45481 atttatcgtt atttggcaaa aatggaggag aaaaatggaa agacaaattc tcattagttt
    45541 ttgtcattta atgctgaaaa tttttgccat ttatctagaa aagtgacaat agtatgagaa
    45601 ttttttgtaa gtgtctactg ataacttatt ctcaaatgtt gtaaggattt tattcatttg
    45661 ttaatat
//
LOCUS       DACDIG010000017        41608 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.017, whole genome shotgun sequence.
ACCESSION   DACDIG010000017 DACDIG010000000
VERSION     DACDIG010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.017"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..392)
                     /locus_tag="IV247_000973"
     CDS             complement(<1..392)
                     /locus_tag="IV247_000973"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8777060.1"
                     /translation="MSISFSNNGRKSFSGKEVQIQYAALDIYWNIFSSTATEFYDEDP
                     QVVAFMMNTFFKKEVLDRLNSGMIDKLYFLLKICKENFPATSVKDVQKEFTEYEYIDF
                     FIDLSVDILRPNLPICREQRILVNILARL"
     gene            complement(399..833)
                     /locus_tag="IV247_000974"
     CDS             complement(399..833)
                     /locus_tag="IV247_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777061.1"
                     /translation="MQTQEAIKTFILKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISFSQQTNYSASYLYTKMKKINAFLA"
     gene            981..2122
                     /locus_tag="IV247_000975"
                     /pseudo
     CDS             981..2122
                     /locus_tag="IV247_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(2248..3327)
                     /locus_tag="IV247_000976"
     CDS             complement(2248..3327)
                     /locus_tag="IV247_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8777062.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(3430..3870)
                     /locus_tag="IV247_000977"
     CDS             complement(3430..3870)
                     /locus_tag="IV247_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8777063.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(3927..4337)
                     /locus_tag="IV247_000978"
     CDS             complement(3927..4337)
                     /locus_tag="IV247_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP8777064.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(4517..4951)
                     /locus_tag="IV247_000979"
     CDS             complement(4517..4951)
                     /locus_tag="IV247_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP8777065.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(5051..5899)
                     /locus_tag="IV247_000980"
     CDS             complement(5051..5899)
                     /locus_tag="IV247_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8777066.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            6288..8054
                     /locus_tag="IV247_000981"
     CDS             6288..8054
                     /locus_tag="IV247_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAP8777067.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPVHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            8156..9415
                     /locus_tag="IV247_000982"
     CDS             8156..9415
                     /locus_tag="IV247_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8777068.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            9562..10863
                     /locus_tag="IV247_000983"
     CDS             9562..10863
                     /locus_tag="IV247_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8777069.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            10866..11717
                     /locus_tag="IV247_000984"
     CDS             10866..11717
                     /locus_tag="IV247_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8777070.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            11730..12545
                     /locus_tag="IV247_000985"
     CDS             11730..12545
                     /locus_tag="IV247_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAP8777071.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            12696..13862
                     /locus_tag="IV247_000986"
     CDS             12696..13862
                     /locus_tag="IV247_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8777072.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            14092..14739
                     /locus_tag="IV247_000987"
     CDS             14092..14739
                     /locus_tag="IV247_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAP8777073.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            14823..15767
                     /locus_tag="IV247_000988"
     CDS             14823..15767
                     /locus_tag="IV247_000988"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAP8777074.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTIQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            15902..17353
                     /locus_tag="IV247_000989"
     CDS             15902..17353
                     /locus_tag="IV247_000989"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAP8777075.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            17374..18210
                     /locus_tag="IV247_000990"
     CDS             17374..18210
                     /locus_tag="IV247_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP8777076.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            18210..18869
                     /locus_tag="IV247_000991"
     CDS             18210..18869
                     /locus_tag="IV247_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP8777077.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            18995..19684
                     /locus_tag="IV247_000992"
     CDS             18995..19684
                     /locus_tag="IV247_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777078.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            19690..20646
                     /locus_tag="IV247_000993"
     CDS             19690..20646
                     /locus_tag="IV247_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAP8777079.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            20690..21202
                     /locus_tag="IV247_000994"
     CDS             20690..21202
                     /locus_tag="IV247_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8777080.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(21458..22006)
                     /locus_tag="IV247_000995"
     CDS             complement(21458..22006)
                     /locus_tag="IV247_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8777081.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(22095..23276)
                     /locus_tag="IV247_000996"
     CDS             complement(22095..23276)
                     /locus_tag="IV247_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAP8777082.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(23512..25020)
                     /locus_tag="IV247_000997"
     CDS             complement(23512..25020)
                     /locus_tag="IV247_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAP8777083.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            25179..25370
                     /locus_tag="IV247_000998"
     CDS             25179..25370
                     /locus_tag="IV247_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777084.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            25573..26490
                     /locus_tag="IV247_000999"
     CDS             25573..26490
                     /locus_tag="IV247_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAP8777085.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            26483..27556
                     /locus_tag="IV247_001000"
     CDS             26483..27556
                     /locus_tag="IV247_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777086.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            27704..28222
                     /locus_tag="IV247_001001"
     CDS             27704..28222
                     /locus_tag="IV247_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777087.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            28203..28595
                     /locus_tag="IV247_001002"
     CDS             28203..28595
                     /locus_tag="IV247_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAP8777088.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            28671..29606
                     /locus_tag="IV247_001003"
     CDS             28671..29606
                     /locus_tag="IV247_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAP8777089.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            29739..30518
                     /locus_tag="IV247_001004"
     CDS             29739..30518
                     /locus_tag="IV247_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAP8777090.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            30511..30828
                     /locus_tag="IV247_001005"
     CDS             30511..30828
                     /locus_tag="IV247_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP8777091.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            31072..32682
                     /locus_tag="IV247_001006"
     CDS             31072..32682
                     /locus_tag="IV247_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8777092.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            32839..33687
                     /locus_tag="IV247_001007"
     CDS             32839..33687
                     /locus_tag="IV247_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8777093.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            33918..34397
                     /locus_tag="IV247_001008"
     CDS             33918..34397
                     /locus_tag="IV247_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777094.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            34430..36658
                     /locus_tag="IV247_001009"
     CDS             34430..36658
                     /locus_tag="IV247_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP8777095.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            36787..38430
                     /locus_tag="IV247_001010"
     CDS             36787..38430
                     /locus_tag="IV247_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP8777096.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(38503..39048)
                     /locus_tag="IV247_001011"
     CDS             complement(38503..39048)
                     /locus_tag="IV247_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAP8777097.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(39071..39556)
                     /locus_tag="IV247_001012"
     CDS             complement(39071..39556)
                     /locus_tag="IV247_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8777098.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(39546..40358)
                     /locus_tag="IV247_001013"
     CDS             complement(39546..40358)
                     /locus_tag="IV247_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAP8777099.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(40497..>41608)
                     /locus_tag="IV247_001014"
     CDS             complement(40497..>41608)
                     /locus_tag="IV247_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP8777100.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT        13828 a         7516 c         8102 g        12162 t
ORIGIN      
        1 agtcgagcta aaatattcac taagatgcgt tgttcgcgac aaattggcag attaggtcgc
       61 aaaatatcta cagagagatc aataaaaaag tcaatatatt catactctgt gaattctttt
      121 tgtacgtctt tcacagaagt tgcaggaaaa ttctctttac atatttttaa tagaaaatat
      181 aatttatcta tcataccgct atttagtcgg tcaagtactt cttttttaaa aaaagtattc
      241 atcataaaag ccactacttg cggatcttca tcataaaatt ctgtagcagt acttgaaaag
      301 atgttccaat aaatatctaa tgctgcatat tgaatttgta cctcttttcc agaaaaagac
      361 tttctgccat tattagaaaa ggagatagac aaaccgtact atgctaaaaa tgcattaatc
      421 tttttcattt tcgtatacaa ataagaggca ctatagtttg tttgctgact gaaactgata
      481 atcgaatttg tctgacagct gaacaaagaa gtcaagatct gataactgac tgattcgttg
      541 cagtatcttc caaatagacg ataatagcag taagccggat caattccttc aaaattcaac
      601 tttaaaaaag gtttttgctt atcaatatgc agttctgtta gattgagacg ctcaaagtct
      661 tgttctaatt ctttgatgta tagtaaaaaa tttgattcag acatatggaa atgatctgct
      721 aaatattgtg tagtaataaa gaaacgatgt tcagatagga aaagaaaaat atctaatttt
      781 gtattgcttt ttttcttcaa gataaacgtt ttaatcgctt cttgagtttg catgaaagag
      841 gctccttttt acttaaattg ttaaaacttg aaaatattac tgtttttttc ataaatttag
      901 acaaaaaaac agtaaacata taggtagaat ctctactatc ttatttcttg catacaaaaa
      961 aatcaaggag tgagagagtt atgagatatt caaccatctt aatatcaaca tttgcagcct
     1021 taagcttatc gcttactata aatcagacag tccacacaga ggagacagaa acagccaaat
     1081 ggacagaaac caagtgggcc aacgtcaatc gaattacctt tcaggatgaa ttttcatcct
     1141 tcaattcagg agtcgcaaaa ttaagaaaag gacaaacagc gaccatcaaa gcaaaaattg
     1201 actattcggg agacttagca cctatactac aatcagctgt tacatttgag aatgttgatc
     1261 ccactttgtc gattggctat gacgagaaca ctttgactat ccaacacaag caagcgatat
     1321 ttaccttcac cgttacatta aatcaggact tgttaaaacc agcgcttttt actgtcaagg
     1381 tatcagatgg actaccgaac gatcatcatt taacgccgta tagtcagcgg cagacaattg
     1441 agcaagattc agccatcgac tcaggaggag atcttgttga agaaccaaca gataaaccag
     1501 aaaatgaaaa taagccagaa gtaccgccta cagaaaatcc agacggagaa cagaaaccgg
     1561 aaatagaacc tggagaagaa cccgatacag aaactcaacc agagccagac aataaatcaa
     1621 aacctgaaat aacccctgga gaaaaaccag atgtaactcc agaaccagat acggattctg
     1681 gaaaccaaac agtaccggaa acaaatccag acacagataa tgaaactgaa aatccagaaa
     1741 aaccagaggt agatccagaa gaaaaaccag atgtgactcc agaaccagat acggacgcaa
     1801 gagaccaagg aataccagaa aaaataaaca aaaaaacaat tcaagaggat ggaaaaaaag
     1861 agagtaaaaa atcaaatcta gcaattctaa aaataaatga agagcagctg aataaaaaat
     1921 cacgaatatt tgatagcgcc caatcagctg aaaccttaaa aagtagcaag gatacaacct
     1981 ttgctgtcct gaaacgaaaa ataaacagct accaaaatcc ggagagtcac aaaacaaagt
     2041 tatactgtgg agcggaatca ttctattaag tatagcaaca atgctttctg caaaaagatt
     2101 taaacaaaac agatctctct aacatttgaa taatgtctgt gaacgcattt ttcaaataaa
     2161 aagaaatatc cagaaaatga ccccaaaata ccagattgaa atctgatatt tggggtcgtt
     2221 catactttat ctttcttcat atttatttta agggatatct gtttcttttg gaagatggtt
     2281 cgggatcttt ttcactttca atacatctaa aaagaaggta aagatcggaa tgccgacaat
     2341 caagccccaa acgccaaaga accgttcgct aacaagtaag accacaaatg tataaaaaat
     2401 cggcagttct gttttgctgg acatcaactt cggattcagc acataagact caattagatg
     2461 gacaatcaaa atcgtaatca ggatataaac aacatctcgg atgcctcctt gggaataagc
     2521 tatgaaagat agaggaatac aagaaatgat aacacctgct actgggatca gacttaaaat
     2581 gaaaatcatg atcgctaaac tcagcagctg atgaaagcca aatacagcca aggcgattgt
     2641 agtgagcaac gtatttaaaa gagcgacaac aaattgagct tctaatacca aaccaaatgt
     2701 attgacaaac ttatcggcaa aatagtaaat atcttgaaag aaccaagaaa attcactttt
     2761 taaaaacaat cttgagaata caattgtttt tttcttttca atcataaaaa agaaacttaa
     2821 gatgaatgac atagcaaacg ccactgccat ttttcctata tcctgtaaat agccaagcgc
     2881 aatcgaagcg ccattttgaa gctgttcaaa aaaatcgttt cgctcaatat aagtatggat
     2941 atattgtaaa acaggatctg catcttttgg ctgatgctcg taaaaattaa taacggactg
     3001 gatcatgtca aatgtttgat gaataagcat cgggatatac tttgtgaatg ccagatagat
     3061 aaggtaaacg atcagtgtat aaatcatgat agctaaaaca cctgtaggga ttcggacaaa
     3121 tcgctgaatg atttgaacaa gatgaatcat caaataagta aagataaatg tgagcaaaat
     3181 ggttgtaatc atacttctaa ctaagaaaag aacaaaaacg acaaataata aaacagtgag
     3241 acggcgtatt ttttcattcg ccaaaaaacg ctgataaacg gtcaaatcga caacctccaa
     3301 ataacaaaaa ataaacacgt tattcattat atgatggaat atgaaaataa gaaagagcaa
     3361 aaagaaaaac cgctaaatga cgttatctta tgagttggtt ttcctcataa aatcgttcat
     3421 ctagcagttt cagccctttt tctggtcttg atgaaaatta tctagcattt ttcgggtcag
     3481 atgttgcagt gtatccaatt cttcctgttc aagtggaaga taagacttga tcttttgtgg
     3541 aataccttct gcttgttttt gcaacgcagc accttgagca gtcagcgtaa catttacgat
     3601 tctttcatca cccgtttcac gctgacgatt aatataattt ttttctgcta acttttttag
     3661 taagggagtc agcgtaccag aatccagaca caaatcattt cctagctctt tcactgaagt
     3721 ctttttcttt tcccataaaa ctaacatcac aagatactgg gtataggtaa gattcagcgg
     3781 cttgaggagg ggagtgtagt gtctaatgat ttctttagag gcggcatata aaggaaaaca
     3841 tacttgatta actaaatata gatcttccat ttttccacct ccgctgtttt actatagtgt
     3901 atcatagcgg atttacacaa aaaagtctat tcactaactg ctttcagagt aacttctata
     3961 tttccagctg tagcttttga atacggacac actttatgcg ctgcctctaa taattcttgc
     4021 gtttcttcca gagaaagacc ttcgatagat ccttcaatct ctacacctaa gacaaagctc
     4081 gtttcacttt ctgaataaag ggaaacttgt gcactgacag tagaagcccc agagatattt
     4141 ttttgctgtt tgatcaattc taatgcactg ttaaaacatg cactgaaacc agctgcaaac
     4201 aattgttcag ggttggttgc attttcgacg cgtttaccag gagctgcaat attcaattcg
     4261 aatgtacgat cgggattatg cacttctcca gagcgtccgc ctgtgttgat cattttcgta
     4321 gaatacattt ttttcataaa attaccacct ttaattatat tttatacaat tgaattttac
     4381 acaatataat tgtggaaagc aaagaaatgg attggcataa gataaataag agaaagcaca
     4441 tgaaacagac gcgctttctc gaatagcaaa catatacaaa aaatatcaga gacaaaaaat
     4501 aatctcatca taaacattac atatatttct caaaaagttg ttttctttct tcaaaaatta
     4561 cttcacgatg gacctgatga agcatccata aataaccaaa aggatctctg aacattgagt
     4621 tagtcacacc aaagtccatc atttctgtca tttcttgaat ctctacacag ccatttcgta
     4681 atgctttatt atgtgtgtcc tcgatatcag agacgatgat attgaaccag atcgtatttg
     4741 gatgttcagc agtaggagca gtcaaaccaa gagacgtgtt ttcatccagc atatggaatc
     4801 tcgtaccata caaggtaaaa atgacttcat tattgcctct agggtaatct gttgcctcaa
     4861 tcagttgtat ttcatcaaag attgtttgat acagttctag tgctttcaaa ctatcattta
     4921 atacaaaatc gatttccgtt cctttatgca taaagatctc ctctcctttt cagtcgaaca
     4981 gttactagat tcttttatta taccagctaa agaaaacgca ttcaaaaaaa tagcggattt
     5041 acttaccaac tcattcgatt ggatgataag taaatccgcg tcccattact tctgctacgc
     5101 tgctgacaat gacaaaggca cttggatcga tttcttctat caatggtttc attcgggaaa
     5161 attcatgatc agtcacaacg atcaataaaa cctcttttga ctttcgatta tacccgcctt
     5221 ttgcctctag cagggtaagg ccacgtccaa tctcagcact taagcgaagg cggatgtctt
     5281 ccaaataatg ttcactcatg accataacag atttctttcg attgatacct gtttcgatat
     5341 aagtcatgac aattgaagtg attgcaatag ataaaatagc ataaagaaac tcttctattc
     5401 caaaaataaa cagattaaga gaaacaacga atgcgtctgt aaaaaataag ccaatcgaag
     5461 tattcaagtg gaaatatttt ttaaaaatga ggggagggat cgtcgtaccg ccgctagaag
     5521 agttgttttt atataaaatc gcaactccta aagcaaaaat cgcacttcca aaaatgacag
     5581 acaacaaccg atctgaagta atcattgttt ctggaactat tgcaatgatt aaaggaaaaa
     5641 ccaagcttcc gaaaagtact ttaaaaaagg gttttttacc taaaaataga agagctagta
     5701 ccaacataac gatattcaac actaaaataa ctatagctcg gtcaaagcct aaagcagctt
     5761 ccagtaatat tcctagaccg ctgacacctc cagcagcaat atgatgaggc cctaaaaaca
     5821 tattgatagc gactcctaat atgaagaggg caaaaataat cttaatgtat gtccatataa
     5881 attgtctcgt ttctttcatt tattcacttc cttaattcat taataatact cttatcttat
     5941 tataacttac ttttcacctt aagttaactt taattaataa aaaaattagg ctagtctaaa
     6001 ttattatgtt tcattcttct agaatatact tctacgccaa aaactgttaa aaaactttta
     6061 ctttagaaaa ttggagaaat tgatcgctta agataaattc aggacactaa atagtacagg
     6121 aagtagacta aaacttttaa aagttttaag tctatctatt tggcaagttt aaaaaataaa
     6181 aattcggtat caaaaaactg ttaccgataa catttttgaa agcgttgtct tttgcttctc
     6241 tatcacttat agtgatgacg tcatattaaa acacaaggag gaaatcaatg gatacagctg
     6301 caatttatca tcgaccggaa agtgaatatg catttctata tacgaataca agatttcgta
     6361 ttcggcttcg tactaggaaa aatgatatca aaaaagtata tgtgctatgt ggagatccat
     6421 ataccataac aactgaaaaa tggtatcaaa aacaacgacc aatgaaaaaa tgtctaagta
     6481 ccaatgttca tgattattgg gaaattgaag tgacgagcga aacaagtcgc ttacagtatg
     6541 cgttccatgt tgttggggaa gataacacgg actgttttta cggtgatcaa ggaatttttc
     6601 cttatcgaga aaatgtgatt actgaaccaa atttttattt tcgaatacct tattttcatc
     6661 aaatcgatcg attcactatt ccagaatggg taaaagaaac agtatggtat caaatttttc
     6721 ccgaaagatt tgcaaacggg gacaaagcaa atgaccccgt acacacttta ccttggggaa
     6781 gtaaagatcc agaccgagaa gactttttcg ggggagattt gcaaggtgtg atggatcatt
     6841 tggattactt gacggacctt ggcatcaatg gcatctattt ttgtcccata tttaaagcca
     6901 catccaatca taaatacgat acgattgatt attatgaggt ggatcccgct tttggcgata
     6961 aaaaactttt aaaaaacttg attgatgaag cacataaaag agggatacgt atcatgttcg
     7021 atgctgtatt caatcatatg ggtgtccatt caccacagtg gcaagacgtt ctcaaaaacg
     7081 gagaaaaatc gatctataaa gactggttcc acatccgttt tttcccagta gattcttatc
     7141 agatgactga cttaccagaa actgctgaaa atatcccata tgatactttt gcttttactc
     7201 cattcatgcc gaaactaaat acagcaaatc cagaagtgca aaaatatttg ctggatatcg
     7261 caacttattg gataaaagaa ttcgatattg atggatggcg tcttgatgtt gctaatgaag
     7321 tcgatcatca tttttggaaa aaattcagag aagcagttac agaaatcaaa ccagacatct
     7381 atatccttgg agagatttgg cattcctctc aagcttggct gcaaggggat gaattccatg
     7441 cagtgatgaa ttatgcattt acagattcga ttaaagatta ttttgcaaaa aagaaaatca
     7501 cagcttcaca aatggtcagt ggaatgaatc atcagcaaat gctttacaga gatcaggtga
     7561 acgaaggaac attcaactta ttagattctc atgatactgc caggatcttg accctttgcc
     7621 aaggtaacaa agaattgatg aagtctgtga tggcctttat gtttcttcaa aaagggtctc
     7681 cttgtatcta ttacgggaca gaaataggga tgactggtga agatgacccg gattgccgca
     7741 aatgtatgat atgggagaag gaggaacaag atctagagct attcggtttt ataaaagaac
     7801 ttgtttctct aaggaaacag ttgtctaaga ttatttctga aggttctact cagtggctaa
     7861 tagtaaatga tcgtgaagat aagctctatt tcacacggaa actagaggga cagatcatct
     7921 atgtttactt taatcagtca aaagaacctt gggtagtaga acaggaaaat gaggtgattc
     7981 ttagtcaaaa ttgtcagttg ttagaagacg ggaaagcaga gatacgacaa tacggatttt
     8041 tgatcatgtg ttgaaaactg ttaccggtaa caacaagaaa gcgtttgcaa atgaagattt
     8101 gtgcccatta taataagggt gtaacagcac gttgaataca aaggaggaaa tgaagatgaa
     8161 aaaaagaagt gtgaaacagt tgggtttagg gttaatctct ttaggaatct taggcggatt
     8221 agcggcttgt ggcaatggta actcttcagc agaaggggaa gccgatgaag gaaaaacgtt
     8281 aacagtttcc gttgattctg gatataaaga ttatataaac gaaataaaaa acactttcga
     8341 aaaagaaaac gatgtgaaaa tcaagctagt agaaaaggac atgttcgatc agttggaatc
     8401 attagcgtta gatgggcctg ctggaaaggg acctgacgtt atgatggcag catatgaccg
     8461 gatcggtgcg ttaggacagc aaggacatct ggcggaagta aaactgggaa atgaagcagc
     8521 ttacgatgaa acagacaaag cccaagtgac ttatgatgga aaaatctacg gagaacctgc
     8581 agtaatcgag acccttgttt tgtactataa caaagattta gtcgatacag caccatcaac
     8641 gtttaaagat ttggagaatc tatccaaaga cagtcgtttt gcttttgagt cagaagcagg
     8701 aaaaaataca ggatttttag ctaaatggac tgacttttat tattcctatg gtttaattgc
     8761 aggttacggt ggctatgtat ttggtgatga tggcacaaat ccttcagata tcggtttgaa
     8821 taatgcaggt gctgtagaag gaatctctta tgcaacagat tggttcaaaa atgtttggcc
     8881 aaagggaatg caagacataa aaagtgcagg agacttcgcc agccagcaat tcatgtccaa
     8941 taaaactgca gcaatcattg acggtccatg gcaagcgcaa acatacaaag aaaacaatat
     9001 caattatggt gtagccaaaa ttcctactct aaataatgga cagccttatc aaccgtttgg
     9061 cggaggaaaa ggatgggtag tcagcaatta tgctaaaaac aaagacctct ctcaaaaatg
     9121 gcttgattat gtaacgaatc aggaaaatca agaaaaattc tttgaaatgg taaatgaaat
     9181 ccctgcaaac caacaagcta gggaaacagc aaaggctaaa aacgatgaat taacaacagc
     9241 tgtgattgaa caatatgaaa cagctcaagc gatgccaaac atcccagaaa tgggcgaagt
     9301 ttggacaggt gcagaaaact tgatgttcga tgcagcatca ggttcaaaaa caccaaaaga
     9361 atccgcagat gaagcagtaa aaacaatcag tgaagccata gaacaaaaat actgattaaa
     9421 atgataaccg tccattttta taaaaaatgg aacaggtgct tcagataaaa gtggcaagag
     9481 cgagatggaa aattatcttg ctcttgttgc cattatctta aaagcagttt gcggtttagc
     9541 aagtttagag ggggcattca gatgcagcaa gataaaacaa gcaatatcaa cgaaaaaaaa
     9601 atcacgcttt gttcacttat tccagggatg gggcagtttg taaatggcca agcttttaaa
     9661 ggtatcgttt ttttcgccat attcgtccta ttcattggtc agatgcttct gggcggattt
     9721 catgcattgg taggattggt tactttggga agtataccaa tggaagatca ttcattattt
     9781 attttgattg aaggcacttt gcaattcatc attacaatca ttttcttgat tttttacggt
     9841 ttgaatcttt tagacgctta tcgagtagcg aagatgcgtc aagagggcaa aaaaatcaat
     9901 cggacgatga aggaagtcat acataatata ggtgatgaag gttttcctta tctattgaca
     9961 cttcccgctt atcttctcat gatttttacg atcattttcc cagtcctagt tacattgttc
    10021 actgcattta ctaattatga cttcaatcat attccaccag ccagtttaat tgattgggta
    10081 ggggctaaaa acttttttag tatcttcttt ttaagctcat atcgtaatac tttttttgca
    10141 gtcttttcgt ggaccgtgat ttggacttta tgtgcaacaa ctttgcaaat cacccttggt
    10201 attctcacag cggttgttgc tcaccagaaa tttattaaat tcaaacgtat tttcggtatc
    10261 attttcttat taccatgggc ggtcccagct tttattacta tcatgagttt ttcgaatata
    10321 ttcaacgata gcgtaggtgc catcaatact caagtgattc ctttcataaa ccagcttcct
    10381 tttgtagata tcagtgcaat ctcttggaaa acggacccga actggacgaa agtagcaatt
    10441 atcttagtgc agggctggtt aggatttccc tatatctatg tgatggttac aggaatctta
    10501 caatcgattt cagaagactt atatgaagca gcaaaaatcg acggagcaaa tgcatggcag
    10561 cgcttttcaa acatcacgct tcctgctgtc ttcctagtgg cagcgccaac atttattaca
    10621 cagtatacgg ggaatttcaa taatttctcc atgatctatc tgtttaacaa tggcggtccc
    10681 ggaagcgtag gcggaggagc tggttctact gatattctta tttcctggat ctataaactt
    10741 acgactggaa cttctccgca gtattctatg gcttcagcaa tcacgttgat tatctccgtc
    10801 atcgttatct gtgtgtcatt gctggtattt aaaaagacaa atgcgttcaa tatgggggat
    10861 taacaatgaa aaaatttcat tcgagtgctc gattccaaaa acgactttct cactttttta
    10921 cttacttttt tcttttaata ctgtcagtca ttatcatcta tccgcttttg atcacagcaa
    10981 gtactgcctt caagtcagga aatattgcag cttttagttt agatctttca agtgactgga
    11041 ctttaggaaa ctttactcgg ctattcagtg aaacacttta cgggacttgg tataaaaata
    11101 cattgatcat ttctgtccta acaatgatcg tacaagtgac tgtagtaact ttggcgggct
    11161 atacttatag tcgctatcgt tttatcggcc gcaaatcaag tttgatgttc tttttagtga
    11221 ttcagatggt accgactatg gcagcattga ctgcctttta tgtcatggct ttcttattag
    11281 aagcgctcga taagtactgg ttcttgacaa tgatctatat aggcggtgga attccaatga
    11341 atacttggct gatgaaagga tactttgaca ctgtaccaat agatttagat gaatctgcta
    11401 aacttgatgg ggcagggcat ttcaggattt ttttccagat tgtattaccg cttgtcaaac
    11461 cgatgctcgc cgttcaagca ttatgggctt ttatgggacc ttttggggat ttcatgttag
    11521 ctaagttcct gcttcgaaca cctgaaaatc agactgttgc tgtcggctta caaacattta
    11581 tttctgatgt gacaaaccaa agagtctctc tttttgctgc tggggcaatt ttgattgcac
    11641 taccgatttg tctattattc ttctttttac aaaagaactt tgttaccggc ctgacatctg
    11701 gcggaacaaa aggataggag cagagtatca tgcaaacatt tccgttaaac tatttttccg
    11761 cattacgtac gccaactcaa gtgtttgcgg gacgcaaact attatcatgg cctaaatttt
    11821 tcttgatatt tgtattttta gtttccttga tggtcatgcc tgtgactctt ttttatgcca
    11881 atcagatcca agcgattcca ttagaacaat ttctatccgt tcattcgctg atcgatgaac
    11941 aaggaaccca aaaattttct gaacttgaat tatctgaaac tgggttacaa gcatcgcaac
    12001 aaacgatagc tgtgacccct gaaattttgg taggtgtgtc tttatctgag aaacaacaat
    12061 ccgatcatgg aacttttatt gattttgaga aggaacaatg ggtgatccaa caaaaggata
    12121 aatctggtat acgccgctat acgatgaatt atagcccatc tttccaacca gattctgtac
    12181 gtacgcccga agatttccaa cgatttttag aaagagaatt ttatgccagt aatcgtccaa
    12241 caatcatttt atcttatagt ttaagtcttg gtcttgtcct atttgtcatg acgagtctca
    12301 ttttattcgg ggcttccttt ttcttgtgga tgacaagaaa aagccaactt tcatccattc
    12361 atacattcaa agagagtgca aatttgatgc tgaatgttat gggaataggt tccatggcag
    12421 cagcagttgt agggtttatc cactttgact ttatcttgat gctaagtgtc caaacaacga
    12481 taactgtctt gctgctttta tgggtttttg tcaaaacaag attcaaagat aagcgtgtcg
    12541 aatgacaagt ttaagcgatt tatgcgacaa tgaatgtgaa aaaaatgaaa aataaagtag
    12601 tttttgaaaa atttctgaaa aaattaaact tcttagttga ttcttttctt ttttagtatt
    12661 aacgtaatag ggtagttttt ttaaggagga aaatcatgtt agagaaaact ttatacaatc
    12721 aattattttc acgttctttc tcgctccctg tcgaagtaaa ttattgggac ggtacaacga
    12781 aacaatacgg agatacggac actcctcccc aaatcaaaat caatatccat gaagaaatcc
    12841 caatgaaaga gattacaagt aacgcatctt tggcactagg agaagcctat atggatcatc
    12901 gaatcgagat cgagggaagt atccaagcat tgatcaacga tgcttatcag caagcaaaca
    12961 gctttttacg aggaaaagat tatctaaaat ggctgccaaa gaaagaaaaa catacgaaac
    13021 aaaaaaacaa agaagatatc catgcgcatt atgacttagg taacgacttt tataaacttt
    13081 ggctagatcc aactttgact tattcttgtg cttatttcaa tacacctgaa gatacgttgg
    13141 aacaagcaca aatcaataaa gtccatcata tcttagacaa actctttatc aaagaaggcg
    13201 aaacgttgct tgatatcggt tgtggctggg gaacattgat gttcactgct accaaagagt
    13261 ataatgtcaa ggcaactggg atcacactaa gtgaagaaca atacgatttg attaccaaaa
    13321 gaataaaaga agaacattta gaagataaat gccgtgtatt attaatggat taccgcgaac
    13381 taaaaggcga aacgtttgat catatcacaa gtgtaggtat gttcgaacat gtaggttctg
    13441 aaaatcttga aggttatttc aaagtggtaa aagatttatt gaaaccaaaa ggaacagctt
    13501 tgatccatgg aatcagccga caacaaggcg gtgcgactaa tgcttggatc aataaatata
    13561 ttttccctgg cggttatgtc cctggtgtta ctgaattagt tgataatatt acaaaaaacg
    13621 atttgcaact gattgatttg gaaagcttac gtcgtgacta ccaattgaca ttagaacatt
    13681 ggacaaagaa ttttcatcaa gtccaagatc aagtcacgaa agaaatgggt gattccttct
    13741 atcgaatgtg ggatctatat ttacaagctt gtgcagcttc atttgaatca agtaatatcg
    13801 atgtgattca atatttgctt gttcaacctg gaaacaatga catcccaatg catcgtgcat
    13861 aattgaggca aaagtactgt ttaaagacta tttaaaaaga agctaagttt tatgatgctc
    13921 cagaagtgaa cttttttgtc taacttctag gattgtctct aaagcttagt ttctttttct
    13981 ttatattttc ataataaaag tttggttctt taatttttaa acatgacaga aaattttttc
    14041 ttcgttatac ttgtaataga atactatttt ataacaaagg ggatcgaatc aatgaaaaaa
    14101 actgttattt atactagttt atttgcagct ttgtttttag ctgcttgtca atcaaatacc
    14161 aatcaaggca catctgggaa tacgtcttcc agtagcgaac agcgctcttc acaggtatcg
    14221 gactcctcaa caaaagcaag cacatcttcg tctagccaga caaaaagttc tagtgaagca
    14281 agtactcagt cagatatcaa agtgtctgca gctcgtgcga tcgaattgtt ccaagataaa
    14341 tacccagata ctgcaattac tagtttggaa ttagatagtg attggggatc ttacttctac
    14401 aagatagaag gtgtcgatga tcaaaaagaa tacgaagcaa gaattgatgc tcaaaatgaa
    14461 aaaatggaga cagagaatcc agaaactcta gatcgagatg aacaaaatgg tcaaaagaaa
    14521 aaagaagatg gtctggatgt tgataaattg atcacgattg aggaagcagc aaaaatcgcc
    14581 gtcgacaaag taggaagcgg tacggcaaca gattgggatt tggataaaga tttaggtact
    14641 acttattggg aagtcaaagt aaaagacggc aatcaaagta catcagtcaa gatcaatagc
    14701 caaacaggcg aagtactgtc aactgaacaa gatgattaag aatttcttcg gaaaacaaga
    14761 gttgattttt cttttagtca tgatacacta gacaaaataa cttgtttgaa ggagcgattt
    14821 acttgagttt agaaacgacc tttgccaatc atatctttgc caatcctttg atgaatgcat
    14881 ctggtgtcca ctgcatgact atccaggaat tagatgaact ggctcattca gaagcaggag
    14941 catttatcac taagagctgt acgatcaatg aacgaaaagg aaatcccgaa ccacgctatt
    15001 ttgatgttcc tttagggagt atcaattcta tgggacttcc aaacttaggt ttttcttatt
    15061 atctagagta tgctcttgcg tatgaaaaag tgcaggaaaa ccaaaatcaa ccgttatttt
    15121 tttccattgc cgggatgagt gtccaggaaa acttggaaat gcttgagaaa atagaaaaaa
    15181 gtgggtttaa tgggatcact gaattaaatt tatcttgtcc caatgtgcct ggaaaaccac
    15241 aactagctta cgattttgag gccacttatg aaacactgaa agaagtattc agcatctttt
    15301 ctaagccgct aggaatcaaa ttgcctcctt attttgattt tgctcatttt gatcagatgg
    15361 ctgatatctt gaatcagttt ccattgacct atgtcaatgc tatcaacagt gtgggcaatg
    15421 gtttgtatat cgatacagaa caagaagcag tagtcatcaa accaaaagaa gggttcggcg
    15481 gcattggcgg agagtatatc aagccgactg ctttggcgaa tgttcgcgct ttttatacac
    15541 gtttgaaacc agaaatccaa atcattggta caggtgggat tcggacagga caagatgcat
    15601 ttgaacatct gctatgtggc gcaagcatgt tacagattgg taccgagctt cataaagaag
    15661 ggccggaaat tttttctaga atcataaaag aattaacgca gattatgtca gaaaaaggat
    15721 atacgtcaat tgacgaattt aaaggcaaat tacgcaccat ttcatagcaa agagacgaag
    15781 gtcatgattg atcttcgtct ttttttataa aaaacactcg tctgttcatt cctgtgcaag
    15841 cgtactcatt gtatgttaga cgaacttgtg ggaaaataac agaggcaaaa aggaggataa
    15901 tatgaatgat gcaacagaat tagcaaaaga gcttaaagaa aaagaattta gttttcaaga
    15961 actagttacg gatatacaga aaaaaataaa gaagcaaaat catgaattga atgcgtttgt
    16021 cgcgcttgaa ccattcgaga gtaattccca cgagctattg attccccagt cagaaaaagg
    16081 gccattggct ggtatcccat ttcctttaaa aatgctcggt caagaaaaaa agggatggct
    16141 agctacttca ggatctcgtt tatttgaaac acaccgagct tctcatacat ctaattatgt
    16201 gcggcaagca gaagcgatag gtctagttcc ttttggacaa accaatgcgc cagagtttgg
    16261 tttcaaaaat attacagacc cagtcatcta tggcccagca agaaaccctt ggaaccttga
    16321 atgtacaccg ggcggatcaa gtggaggagc tgctgctgca gtttcatcag gaatcgttcc
    16381 tttagctggt gctagtgatg gcggtggttc tattagaata cccgcttcgt tttgcggatt
    16441 gattgggctg aaaccttcgc gagggacgat gccagttggt ccatatgctt ggagaggatg
    16501 gcaaggagct gctattgatt tcggcttgac agtttctatg agagacacgg aggctttatt
    16561 ttatgggatg agaagtatcc attccggtgc gccatatcaa gtatcgccag ttgagtggca
    16621 aacacatcca agaaaaaaac ggttgaagat agctttttgt gttgattcac cgatccactc
    16681 acagattagt gaagaggcag tacttgctgt aaaaaaagct gtagatttcc ttggaaaaga
    16741 agggcatgat ttgacagaga ttccttaccc actagatggt cgtaaactga tcacgtctta
    16801 ttatcagatg aatggcgcag aaacagcagc aatgatccgc tcgattgaaa caggcatcca
    16861 gcgagctgtc agaaaagaag aacttgaacc catttcttgg actttgttcc aatatggtca
    16921 gaagctctct gccgctactt acatcgattc tctgcatgta tgggatgaag cagcaattgt
    16981 catggaaaac ctacatcaag aatacgatct attcctttca ccaacgactg catttaccgc
    17041 ccctaaaatc agcgaagatt tgcaaagtga tgcgataaga gcacagatgg aacacatcag
    17101 tgattgttct gaagtagaag gtttagacgt tgtttcttcc atgtttgagg agagcttgaa
    17161 gatcacacct tatacacagt tggctaatct gactggacaa ccagcaatca gcttgcctac
    17221 tcatttgaca aaagaaaaca tgcctctagg aatccaattt atggctgcca gaggaagaga
    17281 agatttgcta tttcaaatcg gctatctatt tgaagcaaat catcagttcc atttaccaaa
    17341 tccatggaaa tagcagaaac aaggagaaga agaatgaaga aaaaaagatt gtttgctcta
    17401 ataactagtg ccttttttat tttaagcgct tgcggtacag ctgccgctaa tcaggaagat
    17461 aacagctgga gtaatgtaga aaaaaagaaa gaattagtag tagcgacttc tggcacactg
    17521 tttcccgcat cttattataa tgatcaaaat gaattagttg gttatgatgt agatttggca
    17581 aaagaggtgg ctaaacggtt aaatgtcaaa atctccttta aagaatacaa cgtggatgga
    17641 caaatcactt ctgtttcaaa aagagaatca gattttgctg cgaacgattt tggtatcaca
    17701 aaagaaagag aaaaaaaatt ctccctttca gagactttca aatattcatt tgacagtatg
    17761 atcgtacgaa aaaaagatga ttcaggaatc cactcacttg aagatcttaa agggaaaaaa
    17821 gcagctggcg aacccaatac tagctatatg cgtttagcgg aaaaacacgg agctaaatta
    17881 gtcacatatg acaatgccac gaatgatcag tatttaagcg atgtagcaaa tggtcgaaca
    17941 gatgttattc taaatgatta ttatctgcag aaaatggccg tatctgcttt accagatatc
    18001 cctgtaaaaa tactggagaa cgtttatttc aatgaaaacg aaagtggttt tttattgaaa
    18061 gaaggaaatg atgctctgaa aaagaaaatc gatggcgtat taaaagagat gaaagaagat
    18121 gggacgacaa agaaaatatc tgaaaaatat tttcaaactg atgtttcagt caaaccgaaa
    18181 gaaacgatgg ctgagtcggt gagtgagtga tgtactttcc tctaattgat ttttccctca
    18241 tgtggacttc acttcctttc gtactgcaag gcttggtata cacattaggg attggcttcg
    18301 tgtcgtttct tttaggaaat ctggtagggt tgcttttgac agttttaggt cttttggatt
    18361 ggctgcctct gaatgtattt attcgttttt atttatcgtt ctttagaggc attcccgcac
    18421 tagtcctgct gtttctgtta tattttgggt taccttatca attaagtgcc ttaacggctt
    18481 cagtcatctg ctttacgatt accagcagtg catttattgg tgaaatctat cgaggatcac
    18541 tagcaggcgt ttcttcaggt cagtgggacg ctggtttagc ttctggtttt tctttctttg
    18601 gggtattgaa atatatcgtt ttgccgcaag cttttcgaat ctcagtaccg gcactcagta
    18661 acgtagctat ggatctagtg aaaggtactt cgttagcagc aacaatcaca gtgccagaaa
    18721 tatttcaaaa agcaaagatc ataggcggtc ggacattcga ttatttttct ctctatattt
    18781 tagttgcact gatttattgg gtcatttgta tcgggatcga aaaagtgcaa cagtatttag
    18841 aaaaacgttc tatgcatcag tcaatctgaa aaatagaaat ggtgctagcg tggctggcac
    18901 catttctatt ttttgtttct tgtatattct gcctagtatt ttaaaaccat ttcatgtaaa
    18961 atgtaaatcg aaaatatgtt cggaggtaga aaacatgaag accccgctaa ccatcgaaat
    19021 agaatcctcg ttataccaat attgcatcga gcaaggcggc ttggttgtag aagaagtcac
    19081 gatgcctgat gaccaaggaa ttgtagatac gcttagctgc ttgacaaaat ttgatggaag
    19141 ccgagaatgg cgatgctatg agctgaaagt gacaaaagca gattttcgct ctacagccaa
    19201 actgtcattt gttggacatt ataattattt tgttttacca agtaaactat atgaagaagt
    19261 aaaaactgaa atccctacag gaataggtgt tttagtgtat cgtccgtaca cggttgaaga
    19321 ggagatgcct gcctctggta catttctgat tgcgaaaaaa gcagcaaaga aagagcttgc
    19381 tgtaccggaa gaagctttga caaaccgctt catggcttct ttattcaggg aagtaagaaa
    19441 agcaaagcaa atggagcgag gcgtaaaata ttttccaaca gatcaattgt acaaagaatt
    19501 gaaaagacgt tcagaaaatc aagatccatt ttctaacgaa cgatattatg aacggttttt
    19561 agaggaaata gctagtcagg caatcaatga gttgcaagaa gaagttatcg cgcttcagca
    19621 tgattatgaa tatttgaggc aacaacagca gatccgtcgg ttgcctacag agccattgga
    19681 gtgaaaaata tgtactatcc ttatttaaga ggaaaacaat ttgacctatt agcattaaaa
    19741 gaagcactgt cgcgaggatt gctctccaat aaaatccaac cagtgatcga accagtacgt
    19801 gattccgcta cgcttaaaaa tgtgatcgaa ttatttcaaa aaaagcacca tcgaatcgct
    19861 gtgatccaaa atccccaagt aggacaattc aaattattcg atcagcatgt gcacagttgg
    19921 acagtcaaag aaaatagctc tgtcgtccct gcacagatct taacgccaga aaatctcagt
    19981 gaagtactgg attcacctcc agcgtttctt gttttcgatg gacagcacta cccaaaagat
    20041 acagaggtgt ggaaacagtt ggcaggagtt gattcgaaat ttttgatacc agatactagt
    20101 cgctttcgca tctggctgcc cgaaaacaaa attgtgatcc gtgactcttt tcaaacgaga
    20161 aagcatgttg aaagttatgc agataaaaca gatgactttt ttagtgatga ctatcttttt
    20221 ttccacgcag acggatatat tggattttct gatttcacga tcgaaggtag ccgttatttc
    20281 gataaagggt tccctagccg agcgcttgct atccatctaa cgtatattga tgcttacggc
    20341 aatatacgcg tgaaacattt tgtctctgac agtaacgact ctgctaaaga ccaagcattg
    20401 aagtttcttg aagcaggaga taaaatgaaa aaatggatca tgcgtcacca tcatcaactc
    20461 ttgattactt caggaatgat tgaattattg gctctttacc agcaacaaaa attccctgga
    20521 ctgggagtgt tgaaaaagtg gtcacttatg catcatttgg aacttattag tcacctgcta
    20581 gatcatccaa aagattggct taaatattat tcaaaagttc aggaactata tgaagtgatc
    20641 caatgattag ataatgtaaa tgaaaaagga atagaaaaga ggaaactgga tgtatattcg
    20701 gcaaacaagg aaagaagaag tagaagaagt ggtagaaatc attaatgccg cagcggattt
    20761 cattgcttcg caaggctctc ctcaatggca aaacgggcag aaaccaacaa aagaaacgat
    20821 tttgaacgat attgaaagaa aagaaagcta tgtattaata gtggaagatc aaattgctgg
    20881 aacagctgca ttagtctcaa gcgtggatcc cgtttacaca aaaatagatg gtagttggaa
    20941 agaatcaact ggtccttatt tatcgattca tcgtgtcgct ttagcagaaa gtactcgagg
    21001 aaaaaaacta gcaaaaccgt tcttagaaca tttgttgacg attgcagcca gcaaaaatat
    21061 ccaagatatc cgtatcgata cttatccagc taatgaaccg atggaaaaga ccatctattc
    21121 tgctggattc acttatcaag ggatgattga atttcctttt gaacacggcg aaagaaaagc
    21181 atatcaaata atcatcaatt gactataaaa tcatctgagc aaagataaag acattaacac
    21241 tactgaaaaa aacaatcata tgatagccaa gaggttgtaa cagaagtggc cagcttcaaa
    21301 aaacaagaaa gaatttccga aaatgcttct caaatttttg tggaatttta gcgtatttta
    21361 cgaaatagct gctttctgaa actcccttca tcgagaggtc gtgacatagc ttttgtcacg
    21421 acctctcggc ttatcctacg agtattattc gatacattta tatcaataat ccttttttta
    21481 ttttatctgc agcttcttgg aacgctccat cgtttgacac gactaaaagt gaggggatag
    21541 ctttcaaacg tttcaactgt gctaaatctt ctacataaac cgccagtctg gctgaaatct
    21601 gttcagatga atctccacgt tttttcaggc gcgattgcaa tgtttcttta gagatcgtaa
    21661 taaagatagg acggactacc gtcccgaaat actcatataa atgccaaaaa ccatttgctg
    21721 tcaaaacggc gtaacaattt cctactgtca gtttcttttt aatttctgct ttactggaac
    21781 catatttatg ttgagcatat tggtcccact ctgcaaactc atttcccttg atcatctgat
    21841 tgaatgtatt gtcagaaaca aaataataat cctcttgatc tttttcccca ggtcttggtg
    21901 atcgattcgt gtaagtaatg atcttttgtt ccggctgaaa aatagcttct gcgagtgaag
    21961 tttttccaga accagaagga ccgataaata catagcaata attcattgtc atttctcctt
    22021 tgatgattga acaagtgaaa aagctttgga aaatatttcc aaagctttta aaagtaactt
    22081 tgcttttttg tatattatac cagcaagcca gtcacaatag cacttaaaaa gctgactaaa
    22141 gtcgctgtta gcaaaatttt caatccgtgt tttgcgacaa gatttccttt ttcttcattt
    22201 aatcccttca tcgcaccaga aatgatcccg atagaagaga agttggcaaa tgaaacgagg
    22261 aagacagata taattgctgt tgtacgctca gtaaaatgga aattaccatt tgctaattct
    22321 gtcatagcaa caaattcatt tgtaaccagt ttagtcgcca tgatactacc ggcatccact
    22381 gcttctttcc atgggattcc gctgatgaag gcaagagggg caaagatgta acctaggatc
    22441 tcttggaaag tgatgccgaa tatcccgtgg aagatagcat tgatcatggc aatcaaagca
    22501 acaaaaccga taagcatagc agcaaccgtg atcgccacat ggaaaccatc aagaatgtat
    22561 tctcccaaca tttggaaaaa tgtctgttct ttgttttctt caatgatcag ctcatcttct
    22621 ttttcttcta atacataggg atttacaatt gaagccagaa tataaccgcc aaataagttc
    22681 aaaacaaggg cagtgatcac atatttagaa tcgatcagtg ccatataaga tccgacgatc
    22741 gacatagaaa ctgtagacat agccgataca ctcatggtga acaaacgttt ctcagatagg
    22801 aagggtaatt ccttttttat ggaaataaaa acttccgatt gtcctaaaat agcagaagct
    22861 actccattat aagattccaa tttgcccatc ccgttgattt tacttaatcc tagaccaaca
    22921 gctttcataa ataatggaag aatcttgata tagcgtagaa taccgataat agcagaaata
    22981 aatacaattg gcatcaatac ggaaagaaag aaaggtgcac ttcctttatt tgctacaccg
    23041 gcaaaaacaa agtcaacacc ttgcccagca aaagcaagaa gatgatcaaa catttttgcc
    23101 aatacagaaa caaccgctgt accaaaagtt gtacgtaata atacgaaacc aagaacaagc
    23161 tgtaaaacga acatcacaat tagtggtttc aatttgattg cttttttgtt actgctgatc
    23221 aaaaaagcaa gccctaatgt gacaaataaa ccaataatac ctaataaata actcaaataa
    23281 tcactcctat tccaaattcc gttttatttt acctgttttg caaataaaat actaatcttt
    23341 agtgaaattt tcattacttt tttagagtga aacgcattca ttttccatta gaaaataaag
    23401 cgaacaaaat aaaaggttta aaaagtgtaa cgtcttagta aagtctataa aaaaacgaaa
    23461 ctatcgatta agctaatagt ttcgttttca acatttataa gtaaactttt tctattggta
    23521 atatagattt tcggaagggt agacaggtgc gcaggtaata tcgattccta gttttttcaa
    23581 tgtttgttca tcatttttat tgaccatcac agtagagtgg gcttgtgctc cctcaagttc
    23641 agctagttta tcataagcaa cttgagcgat tggattcgtt actgcactga ttgataaagc
    23701 gatcaagact tcattgatgc ttagtgcatc caaagaggaa tgaagtgtct ttgatttcaa
    23761 tccttgtatt gtttcaagaa ctaatggtga caataaagga atgtcatctg cgatatgggc
    23821 taaatgtttt actgcattta agataactgc cgctgacgca tccatcaatg aacttgtacg
    23881 cccggtaatg atgacttcat ctggtaattc gattgcaatc acagcagaag gatcttttgt
    23941 aggactttgc gcttgaatct tatctgcata gtcatgggca gggactacca cgtttcggtc
    24001 ttcaggacgt aaaccgactt cttccataat cagtttgatt cgattaactg cttcttcgtc
    24061 gatcaatcct tttttgaagt cgcattctgt ttggaaataa cgacggatga tttcttgttt
    24121 agaggcttct tggacaacgg tatcatccac gatcccaaat cctacacgat tcacacccat
    24181 gtcagtagga gattgataaa cagattcttt accagtaatt ttttcaataa tacgtttgat
    24241 tacaggaaat gtctcaacat cccgattata gttcacagct actttattgt aggcatcaaa
    24301 gtggaaggaa tcgatcatat tgacatcttt tagatcgact gtggctgctt cataagcaat
    24361 gttcagcgga tgtttcaacg gaacattcca tactgggaaa gtttcgaact tggagtaacc
    24421 agctgcatgt cctttttggc tttcatgata cagttggttc aaggaagtag cgagctttcc
    24481 acttcctggg ccaggagctg tcactacaac gattggttta gttgtttcaa tatactcatt
    24541 ttttccaaaa ccatcttctg agacgatttt ttctacgttg actggatagt cttcgatttc
    24601 tgagtgttta tagaccttga tgtttcgtct ttctagttta ttgataaaga ctttcgtggc
    24661 aggctgtcca ctatagcgag tgataacgac actgttgatt aataagccat aaccacgtaa
    24721 ttcatcgatt aaccgcaaga tatccatatc ataagtaatc ccataatcac cacggatctt
    24781 attacgttca atatctccag catagacgca aatcagtatt tcagcttgat ctttcaattt
    24841 ttgcagcaat ttgattttcg catcttcttc aaatcctgga agtacacgtt tcgcatgctt
    24901 gtcgtcaact agcttcccac caaattctaa atataatttg tcatagtggt tcactcgttc
    24961 caaaatatac gctgactgct cttcaatata tttttctgaa tcaaaaccaa tttttttcac
    25021 taggttccct ccttaagtac taaagcattt tttcattttt ttgaggattc gtcaactgga
    25081 agagcgaaat tcctcaaaaa aacaaagaaa atggtttaaa ccaacttgtc tagatagtta
    25141 ttcattggta tgatagtttt tgataaggaa ggtgctacat gaaaaaaaga cagaaaaaga
    25201 aaaacgcata taaacactat atccgttcga tttttaccgg ttatgaaaga atgcttgaag
    25261 atcccgaatt ggaacaattg acgtttactt atctaaacga agaaacgcag ctaactcgtg
    25321 atgagcatca gcgcattcat ttcacaacaa gagatctgcc tagtaaataa aaaataagat
    25381 aaaggtcatc ggattcgatt ctgtcctatc agatggaaag actagttgat cgaaaaaaag
    25441 gctgtaccaa attttggtgc agcctgtttt atggagcatt gcccgctctt ttttataaca
    25501 attttttaca gaaaatggta ctattgaagt attaataagt gaaaaacaga tgagtaagag
    25561 gaggcaagta gtatgaaaga gccagataca aatgcaaatt atgagccaga cgaactagag
    25621 gagacgaatg cggatcagga tacaggacaa gaatcgtctt ctttttacga tatcaaagaa
    25681 aaagcaagca tgaatctttc ccaattatta gaagacttac aggcttttga aaaagctgtc
    25741 cgcgaagaag atatccctga gatctaccgt ttatacaatg gccgattgaa tgaagaatta
    25801 aaaagaagct caaatgaaaa tcacgagatc gatcaattgc tgatgcgtaa gatccatgat
    25861 cgttttttac aggaatttcc ttttatggaa caagtagaaa acatctcgcc aacgatgagc
    25921 tattacaaaa tcggcactta ttaccatgat cggcccacgg tcggtattga tgcaagtctg
    25981 ccagaaattt ttgttttgcc taagatcgat gaagagtggg agaaatattc ccaaccggag
    26041 tcaacggact atgacaaaaa aatcaatgag ctagacgcaa aggtgatcac tgctcagaca
    26101 gaaattgaac gtttagatga acaaataaaa gaaatcaatc gacaaatgac tgatttgaac
    26161 gacaataaag gattcttgaa tcgtaaaaaa atcgaggaag aaattcaaga attagagaag
    26221 aagaagcaag tactatctaa tgaaaaatta ggctggcttc cttatattga gacgcctgaa
    26281 acaatccagc agcaaaaaga agcattgaag caagaagcaa gagcagacca gcttcgtgca
    26341 gccatcgtag aaaaagaaca aaggcaaatc agacgctatt tcggtagcaa agagggattc
    26401 ggtcaagcaa tccatgaatt cttgatgaat tatcttggca atgagaatcc taataatcaa
    26461 tctaatgaag gaggttccga gtatgaatga acacgatgaa gcagaatgga caaaaaatga
    26521 agaacaaaca gaagatcatg aagaaacgaa cacttcattg gaagaacaaa caagtaatcc
    26581 tctggatgac acagaagaaa tccctatgct agaggaaaca aaaatacacg aaaaagaaaa
    26641 agtaactccg acgaatacga atactgcaga aaacacccat gcttacgaaa taactgaaaa
    26701 tcccgggtat ttagaaggaa aagagcaaga tcatcaagat atcgtcagtt tgattgaaac
    26761 agcggaagca gaattagcgg atttacgtat gagaaaagat ttgatcgaat cagattttcc
    26821 acaattatta gaatcatatg atcgttttat attgaatgaa gaaccaatct ccacgatcgg
    26881 aaagacaagc ctgatcgaat acttcacgta tgaattactg aaaccgttga atgatattgg
    26941 tctggaacgg tcaaattcag cttatgacac atggaaaaca agacattttt cgatcaatta
    27001 tacgttgaat ggacaaaacg aattagtctt ttcctttgta ttaccgacca tcgatcaacg
    27061 ataccaagtg gaatcgatga gtctcctaaa cgtatcacca gagacaatgg aaatcaatgt
    27121 ccaagatgac cgggtgcttt ctttgatccg acactggtca gtggatcgag tattctctgc
    27181 agggcagatc acgatattca atcacaaatt gaatcaatta ttagcccatg caagaacact
    27241 tggttttacc gttaatcaga cattactaga caatacgaag ccacttcgtt tgaatcttca
    27301 gagcgaattt gaattgacga atgaagtatt ggatgatatt tttatcgtgg caatgaaaaa
    27361 tccatcctac gatatggaaa agatcaacga aactacttat caagtacgat tagataaagg
    27421 acagtcttta acgataacaa caaacgaaaa agatcaaaca gtcttgtcga tctcatctgg
    27481 agactaccca cgatctgtca ttgatttctt tatcaattac gaattcttgg tcccattaat
    27541 ggtgagaaag tcctgattgg ataagttgga ttaccagatg tcattcagat aattctgttc
    27601 caaaggactt gcggcagtta tccagaataa ttgtcacaag tcttttctgt atgtactaaa
    27661 tggacgtgaa taacttgctg atgaaggagg gatttcatta ggaatgtcca caaataaaat
    27721 agcacagctt agcttattgt ctgctgcttg tgtagcaggt aggatcgctt ttcaatttat
    27781 tccgaatttc cagccaatga ctgctatttt tttatttata gtactttact taagcttaaa
    27841 agatgcgtta attgtcatga gtctttctat tgcgatcagc agtttttatt ttggtgttgg
    27901 accatgggtc atcggccaat ggatcagcta tactgttgtt ctgagtttgt tttcaatcgt
    27961 ctgcttgaga aaaacagtgc aaaggaatct atggttaaag ggagtctgtt tttttctggc
    28021 gggcatggtt tacggaatag gtatgaccgt ttttgatacg ctgttatatc gtttgcctca
    28081 accttggatc tattaccttc aaggggtgtc gtttgatttg atgcacagta taggcaatgt
    28141 tgtgttcttt cttgtttttc tgccagtcgt caaacgtttt tacaatcatt caggaggaaa
    28201 aaatgaaaaa aataatttat agtttgcttg caatgagtgt cagtatactc attttgacaa
    28261 gttgcagtac aacctataca caatcggctt ccaaccaaaa ggaggaagta aaaacatcgc
    28321 aaaaagcaac aatcacgtta caagaaaatg gtcaaaactt ttcggaaaaa gaagtgttct
    28381 tcaaaaaagg cgatgattta ctgagtattt tgaaacgaaa ttttgaagtc aaagaagata
    28441 acggcttcat cacttcgctt gaagggcact cacaggatga gaaaaatcaa ctctattgga
    28501 tgtttactgt ggacgggaaa agtgctacta ctggtgccaa agagatcaaa ttgaatgatg
    28561 gacaatcggt cattttcaat ttgtctaaac tataagtttc ataaaaaact gttaaagggc
    28621 gaatattgcc ataattcagt gagttcagtt gatgaaagaa ggaaaataac atggaaaatt
    28681 ttaaaaaaag cggaatgttt tccgtaattg ctgctctagg ggctaatatt ctagttgcta
    28741 tcagcaaatt tgtcggctat gcaatcagtg gcagtgccgc catgctaaac gaaagcatcc
    28801 acagtatcgt tgattgcagc aatcagattt tgctgttatt cggtgataaa caagccaata
    28861 aaggacagag cgagttgcat caatttggtg aaggacgagc aaaatacttt ttcagtacaa
    28921 tcgtcgcaat gatgcttttc ttcggcggtg gggcacttgg ggtgatggaa gcttttgaaa
    28981 agttgacgca tccatcccat gaagtgggta atccgatcat tgttatagca atactgttat
    29041 ttggtttggt aatcgaagga agttcgttac gcgtggcaat gaaagaaatc aaagaattga
    29101 atactgaaaa actttccacg atgcgctttt tgcgcgaaag tcgacacagt gaaatactga
    29161 ttatttttac ggaagacttg tgtgctgtaa tcggtttggt cttggcttta gcaggaacaa
    29221 tattgactta tgcaaccggg attgctgcat ttgatgcgat cagcggattg ctgatcggtt
    29281 tcttgctgat gggagccgcc attttcctag caaaagaatt ttatagtctg atcatcggtg
    29341 aaagcgtgac agcaagcgat ttatcaaaaa tcaagttagc ttttgaacgt cctgagatca
    29401 gtcggctgat cgatataaaa acagttcatt taagtccaac ggagatttta gttgcagcaa
    29461 aagcagatat cgttggtaac aaagagaatg aagcttactc ggtcatcaat gatattgaaa
    29521 aaaatatgcg tcagtctctt cctgacaaga agatgtatat atacattgaa acagataaat
    29581 atgacccaaa ttattctcgt aaataaaacg tttattcatg aattgaagca aaaatgagaa
    29641 aaatataatc ttgtttttaa ataaaacggc tacaatttag cgaatttatg ttacaatgag
    29701 tccgctgttt atttgttgta attaaaggag atattttaat gaacaaaaag aaaagtgcgc
    29761 catggcttca agctttgcat gcagtgatgc cactttgtat aagctatata ccagtaggat
    29821 tagcttgcgg agtattgttg caaaaagttg gatttgatcc tctcttatca ggactgttat
    29881 cgattctggt cttttccggt ggcgcgcagt ttttagttgc atctatgctt acgacgcaag
    29941 catcatttgc aacgacatta ttgatggttt tctttttaga attacgctat acattattag
    30001 gagcaagtct tgccggtttt atgaaaaaag aaaagcggac gtttttggct gtcttttctc
    30061 aatcactgaa tgatgaaaac tatgcggtga actacttaaa gttttcaact gacccttcct
    30121 ggaataaaag aaaggcttta tatgtcaatt ggttctcaat gactgcatgg gccggtagca
    30181 atatgatcgg taattttttc ggctcggtga ttcgagtaga tgctgacctt gtccattttg
    30241 ctttgacggc catgttcatt ttcatgttta tcatgcagat gaaaagcgca ctactgatct
    30301 ttacaggttt attctcaggc gtattagggg tcgtattcat ggtattattc caaaatactt
    30361 tcggattgat tgcagcaaca gtcattgctt catttgccgg atttatgctg gagaaggcat
    30421 tcaaaaaaga aaaagctaag aaactaagag accgcggtaa agatgtcaat gagccgctct
    30481 tcaacttccc ggaacaggag gccaatcacc atgactaaac tatatttgct acttttagtt
    30541 gcttgtctgt tcatcgtggc atatatcccg cgcttgttcc cgatgcttta ttttactcat
    30601 cgaaaagtgc catcctggtt cagtgactgg atgaaatatg taccggtggc attatttgcg
    30661 gcgttagctt ttaaagatgt tttcattact catgaacatc tggatattgc ttggaatatc
    30721 aaaatagctg caatgattct agtagcaggc gtggcttaca aaacacgttc gatggcgttg
    30781 tcagttttga caggacttgc gtctgtcttt ttgctgtcta tgctttaaac tgttttattt
    30841 tattaaaaac agagattgcg acataagttg ttcaaactga tctatccgaa ctgcatttgc
    30901 aacagtcaat tctggatgca agtttattcg tagggataaa aaagattagt gttggcttat
    30961 gatgcaagct cttttttatt ttaggaatta gagagggatt tgtccttcta tgattttttg
    31021 ctataatagg gaagatgttt attttatgaa agaatagttg agggaattta aatgaataaa
    31081 aaattgatgc aaggaacatt ttggctaacc tttgctaatc ttctatgtaa agttttaggg
    31141 gtagtctatt tgattccctg gctaagtatg atggggaaca atcaagatgg tatgcttgct
    31201 acgacattgt ataatgttgg ctatcttcct tatggattgt tcctgatgct tggtacagtc
    31261 ggatttccaa atgcgattgc aaaaaaagta gctgtagcca caaaagaagg agataacgct
    31321 gcttgtcggc tgatttttag aagtacgatc aatatcatgt ttgtcatcgg tatcgcctct
    31381 gctgcactga tgtatttatt tgctccttta ctagccggaa tcagtccgat tgctaacgtt
    31441 aataatggga tactagctat cagaagttta tgcccatctt tgattgctat cccaattctt
    31501 agtgcaatga gaggatattt ccaaggaaaa aatgatctgc ggccttatgg aacttctttg
    31561 attatcgaac aggctgtgcg agtgatcgtt atcttggctg gtacttatta cttgcgcgta
    31621 ttgactgacg gaaccatatt agaagctgta ttgatcagta cagtcgcttc tttcttcggt
    31681 gggttagctg cgattatcca tatgtatatc gtcggacgca gaaaagactt cttcgaactt
    31741 aaagattttt tgatttccag ccgttatttc gaaagagaaa accgatctgc ctctgtctct
    31801 atcattcggg aaacactacc gtttattttt gtcggctcag tcattactat cgttcaaatg
    31861 atcgaccaag taacgatgaa accactgctt cactttttcc gtccagagat cgcagatcag
    31921 caactggaat tcttattcag ccgtgcttct gtcaatccaa ataaattgac attgatcctg
    31981 atctccatgg taggaacagt agctataagc agcttgccaa tattaagcac gatgaagaaa
    32041 aaagatcgga tacaaatcga aaaaacagta ggagatagtt tttcgattgc gttattgatt
    32101 cttcttcctt cattgactgg aatgtcttta ttagctggac cactatacac attgtttttt
    32161 ggctatgatc ctgaaagtgt cggatatttc caaatggctt tgctagcttc tttgtttttc
    32221 tctttattca cgatcctatc tacgatgctt caatctttga atcaccatcg ttttgcgatc
    32281 cgattaactg tagaagcaat tatcctgaaa gtcatcttcc aagtaatcgg attaggatta
    32341 gttggcggat acgggatgag cttatcaaat ggcttagcat tcggtattgt ctttgttcgc
    32401 ggctatcact ttatgtgcaa agaataccga atagcacctt tagcaaagat cagcaatttt
    32461 ttcttaaaaa ctttccgctc caccctgatt atgcttgcag tatgttttgc cgttttcttt
    32521 ttgctgaatc agcgattgtc aatggaatca aaaagctatg caatgattta ttgtgtggct
    32581 gtaggaagta ttggcggctt agtattttta ttttctcaat tcggaaaaaa tgggtttcga
    32641 atgttgaaaa atgttaaaca tcatagcaga agcaaagaat aggttaacta atcgtttttt
    32701 gctgtcactt gaaaaacggc aactcgtatg attacgcttg tcgtttttct attttccaat
    32761 tttagtttca tatctaatct tacttatgat atagttataa ggtctggaaa aattctttga
    32821 aacgaggaaa ttaaacacat gaaacttgca gtagttacag atagttcggc atatctgccg
    32881 gaacgtatca agaaccatcc cgacttattc gttattccaa tccctttgat tatggatggg
    32941 aaaatttata acgaaggaat cgatatcgaa gcggatgaat attatggact tttaaaaaac
    33001 agtaaagatt ttcctactac ttcacaacct gtcgtagggg aagttttggg tttatatgag
    33061 gaattgaaag agaaagggta tgacacgatt ttaagtatcc atctttcttc agggatttct
    33121 ggctttgtca acacattatt tgcgatgaaa gatgacatca gcggagtaac ggtcattcct
    33181 tatgactcta agatcactag tatgccgatg ggacacatgg tagaagctgc cttagactta
    33241 aatgatgaag gtcagtccct tgatgaaata cttgcacata tcgatcgaat ccgagataat
    33301 acctacgctt atctgattgt agatgatttg aacaatttag tacgaggagg acgtttgaca
    33361 aatggtgcag cgctgattgg cagcttatta aaaatcaaac cgatcctgac attttctgaa
    33421 gggaaaattg ttttattcga aaaaatccgt tcaagtaaaa aagcatttgc tcgagctgaa
    33481 gaaattattg gtagaagaaa taacgaaata caaccgccag tcaaacttta cgtgatacat
    33541 gcaaataatt tagaagtagc gattgcggag aaagagaaat tgcagaaaaa atatccaaat
    33601 gcagaaatcg aaattggtca tttcggacca gtcattggta cacatttagg tgaaaaagcc
    33661 atcggcattg ccatttcagc gcaataattc cttttattca aaaaacatac caaaaatatt
    33721 ccaacatcat cttggacttt ctacatgtta ctaatgagcg tgtaaaagaa cgagatggtg
    33781 tttttatttc tatcaatgaa gctctttttc tttgcaatcc tcacttcaat cctctataat
    33841 gtcgcatata gtttagtaaa tgagtaaact actaatttac ttatttacta aaaataaaag
    33901 gatgaggagt taaaataatg gaaataccaa caaatttaaa acataaacca gtaatcgttg
    33961 tagaagacta tgataaagtt gatggaagaa atgcattaaa tacggatgca aaagggcttt
    34021 cactgggcct tgctcagtgg aatgatcgtg gaaaagtcga tatctcaggc aaggtatggc
    34081 gacatactgg agaaaaatgg tcacgacagt cggaagaatt accaatcaca cgtattttag
    34141 atttagcgat cttagctgca caaggaagtt tatacttcca agatgcttat cgttatgaaa
    34201 aattttatga tccagaaaat ccagtcgtag acatcattgg attacaaggg aaccgaatga
    34261 cggtagaagt agataccaaa aatccaaaga tcgatgaaga catcattttg tattacgata
    34321 cactacaaaa agacggagaa ctgattgggg aacgattccg tattttaaaa cgtcttttag
    34381 atgagctagg atattaatag acagagatga tcaaaagggg aagagaagca tggagaattt
    34441 ctcactaaca gaaaaccgaa ccttttcacg gctagaaaag caaatgatct ggaagaaacc
    34501 gaaaagccat cggacaagtg cagaagaatg gcggatcgcc tccgaaatca aagggaattg
    34561 gcaagatcct gaaatgaaaa tcagtaacgt acttttggaa ggagatgccg ggtcggggaa
    34621 gacacaatta gccaaagcac tatctgctga tctgcaactt ccctatacaa aagtgacctg
    34681 ttttgcagat atggacaaat cagatgtttt cggagcctta ttacctgtag tagattctaa
    34741 taaggaacaa gaccaagaat tactagaagc tatttaccaa acagatacac ttgaagctgt
    34801 actgacactg atccagaacc attacggcat tgatcctttt tctgctaaag aaaaattagc
    34861 agagctagtt caaaggatcg aagctgatta tccagatgca atccattaca gctattaccc
    34921 ttctgaaatc gttcgagcga ttgaaaaagg atatttatta gagattcaag agccgactgt
    34981 catccgagat gcctctgttc ttgtggcact taactcagcg ctcgaaccaa atgggatgtt
    35041 gaatttaccg acaggaatca ttcgtcgcca tccagattgc gtcattgtga tcactacaaa
    35101 tcgaaattac caaggaaatc gcccattaaa tgaatcttta agagatcgga tgcaacatgc
    35161 ggaaaaaatg gatcttcctg ccttagaagt catggcagaa cgtgcgatgg cgaaaactat
    35221 gatccaaaaa caagaacttc tcttaaagat ggcagagatt atccgtttgt tggatgaaac
    35281 tgcaaaagct aacgccatca aaggtgtagc aggaatgcgt tcttattttt attgggcaaa
    35341 tacctataag caaggacaag atttactcca gtcgatttat ccaaaagttt tgtataaatt
    35401 atccactgat tcagatgaat tggcaattct tgaacaagct ttgtctgaca gtggattgct
    35461 actggaactt caggacatat tgagaaaaga aaaatggggt catcctcaat ctgagcatac
    35521 aaaaggaaga accattagcg ccgaagaagc aaatgaacga aacatcactg aaaatgctga
    35581 agaaattctg cgcttagatg aaaagaaatc gttgtctgct gaagaaccaa atgagggaga
    35641 agagaaacaa gaagaaacaa tagaaaaaaa cgagattcct cctcaaatgc aaaaagaacg
    35701 cagtagcgat atcgaaggaa aaaatcaaaa agaacaacaa acaggagatg attctcaaga
    35761 aatgtctgct tctgacatgg aagcacatga taaggaaatc aagaagcaac tgaataaaga
    35821 agcgcgacaa atcatgaagg aaacgattca tgaaaaagaa ggtctcatcg ttcaccgacc
    35881 agctttccaa ggaaaaacgc cggaagcacg tgagctacac atgcaagtaa tgccaatagt
    35941 agaatcactt agccggcaaa ttttagagtt actggataat gaacaaacag agacgtacca
    36001 aaaaggaaaa tacgaaggtc agcggttcaa tgcaagcaaa gtagcatatg gtgatttgag
    36061 aaattttgac aaaaagaacc cgccacatga gcaaccttct ttagcggtag ctgtacggat
    36121 cgatgaatct gggtcaatga tacgggatga tcggatcgaa gcggcaaaaa aagcagcgat
    36181 tgccattgcc gaattttcta agaaagtcga tatcccattg ttgatttacg gagatacagc
    36241 agaccgttcg tcaagagaaa agacttcttt gttttcttat aaagaatttg aagacggatt
    36301 tcaatggtta aatgaaaaat tagtcactat gaaaccaagg caaaacaatc gtgatggtgc
    36361 tgcacttcgt ttagcagcag aaaaattaaa cagacaatcc gcaacaacca aactgttgct
    36421 taatattagt gacggtcagc caaaagcgtt gccggattat acaggcaaaa aagcaaaaga
    36481 agatatccag tctgttttga aagaatatga acgtcaagga atcctatttg tctccgcagc
    36541 tattggccaa gacaaagaag aaatcaagtc gatctacggt gaaagtcgtt ttgtagatat
    36601 tactgacttg aatgaatttc ccaagcaaat gattcaactg attgcccgct atctatgata
    36661 aaataaagct caacgaacgc acttagcaga ttcgttgagc ttttcgtttc tagttaccgg
    36721 taacaaggat acttctaaaa tagaatcggt tataatcaag agggaccgtt aaaggagtga
    36781 aacgatatga tacaagaaca aaaatggtgg aaagaagcag taggctatca aatctatcct
    36841 gctagtttca aagatagcaa tggcgatggt caaggagaca tcaatgggat ccgtgaaaaa
    36901 cttaattatt tgaaagatct tggtatcggc tttatctgga tcaatccgat ctatcaatct
    36961 ccatttgtcg ataatggtta tgacgtaagt gactatcaag atatagaaga gagattcgga
    37021 acgatggaag attttgattt attgctaaaa gaagcacatg aattagggat caaagtgatt
    37081 atggatcttg tcatcaacca tagttcggat cagcatcaat ggtttgaaga atcgaaaaaa
    37141 tctaaagaaa gcccgtatcg cgattattac atttgggtcg acggggtaga tggcaaagaa
    37201 ccgaataatt ggacatcgat tttcggcggt tctgcttggg agtattctca tgaaactgga
    37261 cagtattatc tgcatgtttt tgcaaaagaa caaccagact taaattggga aagcgagaaa
    37321 ttgaaagaag aactattcaa tatgatccga tggtggttgg ataaagggat agatggattt
    37381 cgtttagatg cgatttctca tgtaaaaaaa gatgaatatt cagtaaaagc cacagagaac
    37441 cctttctcac cctttcaaaa tgtctcagga atcgaagaac atttgaccga tttgaaacat
    37501 gtttttgaag agtacgatat catgactgtc ggagaagcaa gtggtgtcac tgcagaagaa
    37561 ggaccacaat gggtagggaa agacggttat ttcgatatga tattcgaatt tgatcatatc
    37621 catatctggc agcaagaaaa agaagggcaa ttagatgtcc ttaaattgaa acttgcatta
    37681 agcgcttggc aaacttcatt ggatggtatc ggatggaacg ctttatatat ggaaaaccat
    37741 gatgttcctc gtgccgtttc agtgtttgga gatactcgtc ccgatttttg ggctatgtct
    37801 gcaaaagcaa tcgctatgat gtatttcttt ttgcaaggta cgccatttat ttatcaagga
    37861 caggaaatcg gaatgacgaa tatgcccttt gaatcgatcg atcaagttga tgcagtagat
    37921 tcgaaaagac tttataaaag gctgcttgcg gaaggtaaga caagagaaga agcattggat
    37981 atcattcgtg aaacaacgag agacaacagc aggacaccca tgcaatggac ttcggaacaa
    38041 tatgctggtt tttctacaca tgaaccttgg ctgatcacta atccaaatac caaaacgatc
    38101 aatgtagaac aacaagaata tgaaccagag tcggtcctgc aattctataa gaacatgatc
    38161 cgcattagac aaaccaataa aggtctgata tacggttctt ataaagaata tcttcatgaa
    38221 catccacaac tttatgtcta tgaaagatat ttagaagatg aacaatatct tattatggta
    38281 aacttaacag aatcacttgc tgactatgaa cttccaaaag aagcagatca gtcctggaca
    38341 ttactcttgt caaattcctc tagcggagaa tttgaagcta aaggcatact agcaccttat
    38401 gaagctcggt tatataaaac aaacaagtaa aacttcctcg ttaaagttca ttagttggac
    38461 tttgcgagga agtttttcag attgctctgt caattactca tttcaataat tcgctctagg
    38521 accaccgata tatttaggac gagagcgaag ggctgtcacg tgagcttgtc caagggtttt
    38581 caaacgaggt ctgacaggta cttggacatg tttgatatgc ataccgattg atgtgtcacc
    38641 gatatcgata ccggcttgtc caactacgtg ttccacttcg acaggatcat tgaactggtc
    38701 aaaagcagca acagaacaag cacctccagc atgtagtgca ggaacaacag agacaatttc
    38761 gaaatttttt gcttctgcca ctgttcgttc tactactaat gctcggttca aatgttcaca
    38821 accttggaca gctagcgcaa tttctttagg atccaacaac tcttttaacg tacggatgat
    38881 ccattgacca acttccgcac tggaattttt tccaatatgt cctccaacga cttcgcttgt
    38941 gctgcatcct aaaacaaaaa gatcaccttt tttcaagtgt gcctcagcca atatgtcatt
    39001 aactatttca gtcaattggt cttttaattc tttttctgac aacttcattt tctaaaacct
    39061 tctttttcaa ttaatattgc ttccgtgctt gaatacgttg aagacttgca gttataggaa
    39121 aggcaagtcc cattccaatc agattttgga agatgtttcc tggtatagaa gccactccag
    39181 ccggccaatc atataaaatc caaccggcga taccatagcc gatgatcata actaagcttc
    39241 ctaataaaag ccctaatccc cacatacgag tagaagattt gctgaaagaa ccagcaacat
    39301 atccttgtaa cccgtggatc actaatgaaa aaactgccca ctgcggatat cctgaaatca
    39361 aatcgatcaa tagaccactg gaagcaccaa caactaatcc acccgttgga ccataaagaa
    39421 aagcagcggt atagatcccg gcctcgcata atgtcacaaa tccattcgta gccggtacag
    39481 ggatcaaaat tgttaatgaa agggcaactg tcaacgcaat cagcatagca tagatcgtta
    39541 attgcttagt tttcatttct cattcctcct ttttgaatac cgccagacca gacacttccg
    39601 gtatgatcgt tcatcagcac gccattagaa atacaatggg caacaaattt tttactttct
    39661 gctactgctt tggtaaaatc tgcccctaac acaagattcg ctgttattgc agaagaaaag
    39721 gcacaaccgg ccccgtttac tgttgtagtg tcgatttttt tatgagaaaa tacctcataa
    39781 tgatcaattg aatagagaag atccacagct tcatctccag ccatccttgt gccgcctttg
    39841 atcacaatgt tttttgttcc tgaagcagtg agttctttag cgagatggat catttctgtt
    39901 aacgaagagg gaagagcttg accagaaagt aaagcagctt ctgccaaatt aggcgtaatc
    39961 atcgtaacaa gtggaaaaag ttcttcgatg agctgcgaaa tatacaattg attgatcaca
    40021 gcttcagttt ctttgaatgc caaaacaggg tctaaaatga ttgggatatt ttgttttgtt
    40081 tttatgaagt ctcgaacaac catgattgct tcacttgaat tcaataaccc aattttgata
    40141 ccatctaatg aagtcatttt atcaatcgaa actaactgtc gtttaagcaa ttccaaagat
    40201 atagattcca gcataaatcg ctcattaata tctagtgccc ctatagaagt caaggcgctt
    40261 agtccgaata gaccaaaatt ctcaaaagtt tttaaatctg tttggattcc tccaccagca
    40321 aatggatcag aaccgccaat tgtcaagatt cttttcataa cattttcctc catgaataag
    40381 agtataacga aattttcaaa aaagaaaaac agccaattgg catattttta tctcatccaa
    40441 tttaaaaaaa caaaaagaaa agaaagacca aaatggatct ttctttcctt aaatagttag
    40501 gacttttgtg attttaattc tttctcgatt tgaagccgtt gcactttcat tgtagctgtt
    40561 cgcgggattg catcaaaatc acggatgata gggacgttca agcgaggaag ttcatggact
    40621 tgctcccacc atcgattcca atccatttct ttatcaggag ctaaagcaat aatcggttgt
    40681 ggagaatgat ttttatccct tacaatcact acttctgcca aaaaatctaa cgaatctaat
    40741 aaaaagtctt caatagctaa tgtactattg atcgtttcga tcaaatccac ttgccgatct
    40801 ttcaaaaata ggtggccatg cttatccata aaaccgtaat ctccgctatc ccaccaatcg
    40861 ccataaacat tttcctggaa ccgtgcatct tccttgtaat aagtcaaagc ccgtccttta
    40921 gacagtaatt gtatatgacc atctgtgttt tcaggtagga cattcccaac tgaatctgta
    40981 atccgagcgc ttgtcatgtc ttccagtcca acgcccatat ctcttgcatc acttgttttc
    41041 aaactctcta gtgtatgagc cttaagaatc attggtccgc attcactttg gccatatact
    41101 tgaagaaata ccgcatcctg tgccatcgac gttcgcaaaa atgtcttcat tgtctggttg
    41161 tttatagcat caaaagtgga gtgatagtat cggatacccg aaaaagcttc tggatgctcc
    41221 ttagcagtaa aacgccactg aacaaagtta ttcggatggg tctctagtgc aattggccgg
    41281 tgagcttcca acatatgaac aacttttgaa ctttgtgcgt ttgctaaagg catcatagga
    41341 aatcccatgg acatcaaaga agagatacca atattgaatc tggaatgcac tggagagatg
    41401 tgaaaagcga ttaatttctt ttccgaaatt tttgtgaaaa ctgttttttg ccatttcgtc
    41461 cgccatccca tagaatgagc agaatgacaa ataagtttag ggataccagt tgtaccagaa
    41521 gtatgggtca tataagaaat ttcatttttg tctaaaaatg attgagaaac agacaaagca
    41581 ggttgttcca ttaaataaag aataggaa
//
LOCUS       DACDIG010000018        41571 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.018, whole genome shotgun sequence.
ACCESSION   DACDIG010000018 DACDIG010000000
VERSION     DACDIG010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.018"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(135..2516)
                     /locus_tag="IV247_001015"
     CDS             complement(135..2516)
                     /locus_tag="IV247_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP8777101.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2506..3153)
                     /gene="recU"
                     /locus_tag="IV247_001016"
     CDS             complement(2506..3153)
                     /gene="recU"
                     /locus_tag="IV247_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAP8777102.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3217..3756
                     /locus_tag="IV247_001017"
     CDS             3217..3756
                     /locus_tag="IV247_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAP8777103.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3846..4265
                     /gene="gpsB"
                     /locus_tag="IV247_001018"
     CDS             3846..4265
                     /gene="gpsB"
                     /locus_tag="IV247_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAP8777104.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4287..4654
                     /gene="rnpB"
                     /locus_tag="IV247_001019"
     ncRNA           4287..4654
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IV247_001019"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4863..6029
                     /locus_tag="IV247_001020"
     CDS             4863..6029
                     /locus_tag="IV247_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAP8777105.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6075..7571
                     /locus_tag="IV247_001021"
     CDS             6075..7571
                     /locus_tag="IV247_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAP8777106.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7652..8335
                     /locus_tag="IV247_001022"
     CDS             7652..8335
                     /locus_tag="IV247_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8777107.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            8980..10170
                     /locus_tag="IV247_001023"
     CDS             8980..10170
                     /locus_tag="IV247_001023"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAP8777108.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10272..11225
                     /locus_tag="IV247_001024"
     CDS             10272..11225
                     /locus_tag="IV247_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAP8777109.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSGNVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11294..12643
                     /locus_tag="IV247_001025"
     CDS             11294..12643
                     /locus_tag="IV247_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8777110.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(12826..13149)
                     /locus_tag="IV247_001026"
     CDS             complement(12826..13149)
                     /locus_tag="IV247_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777111.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13278..14441
                     /locus_tag="IV247_001027"
     CDS             13278..14441
                     /locus_tag="IV247_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777112.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14511..15860
                     /locus_tag="IV247_001028"
     CDS             14511..15860
                     /locus_tag="IV247_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP8777113.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(15905..16336)
                     /locus_tag="IV247_001029"
     CDS             complement(15905..16336)
                     /locus_tag="IV247_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8777114.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            16724..19366
                     /gene="alaS"
                     /locus_tag="IV247_001030"
     CDS             16724..19366
                     /gene="alaS"
                     /locus_tag="IV247_001030"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAP8777115.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKEHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19566..20162
                     /locus_tag="IV247_001031"
     CDS             19566..20162
                     /locus_tag="IV247_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8777116.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20284..21432
                     /locus_tag="IV247_001032"
     CDS             20284..21432
                     /locus_tag="IV247_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP8777117.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(21470..21883)
                     /locus_tag="IV247_001033"
     CDS             complement(21470..21883)
                     /locus_tag="IV247_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAP8777118.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            21962..22393
                     /locus_tag="IV247_001034"
     CDS             21962..22393
                     /locus_tag="IV247_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAP8777119.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(22492..22725)
                     /locus_tag="IV247_001035"
     CDS             complement(22492..22725)
                     /locus_tag="IV247_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAP8777120.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            22908..24578
                     /locus_tag="IV247_001036"
     CDS             22908..24578
                     /locus_tag="IV247_001036"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAP8777121.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            24725..25111
                     /locus_tag="IV247_001037"
     CDS             24725..25111
                     /locus_tag="IV247_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777122.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            25098..25796
                     /locus_tag="IV247_001038"
     CDS             25098..25796
                     /locus_tag="IV247_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP8777123.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            25801..26265
                     /locus_tag="IV247_001039"
     CDS             25801..26265
                     /locus_tag="IV247_001039"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAP8777124.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            26262..27173
                     /locus_tag="IV247_001040"
     CDS             26262..27173
                     /locus_tag="IV247_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP8777125.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            27408..27947
                     /gene="pyrR"
                     /locus_tag="IV247_001041"
     CDS             27408..27947
                     /gene="pyrR"
                     /locus_tag="IV247_001041"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAP8777126.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            27961..29244
                     /locus_tag="IV247_001042"
     CDS             27961..29244
                     /locus_tag="IV247_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAP8777127.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            29257..30183
                     /locus_tag="IV247_001043"
     CDS             29257..30183
                     /locus_tag="IV247_001043"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAP8777128.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            30202..31482
                     /locus_tag="IV247_001044"
     CDS             30202..31482
                     /locus_tag="IV247_001044"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAP8777129.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            31487..32563
                     /gene="carA"
                     /locus_tag="IV247_001045"
     CDS             31487..32563
                     /gene="carA"
                     /locus_tag="IV247_001045"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAP8777130.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            32568..35753
                     /gene="carB"
                     /locus_tag="IV247_001046"
     CDS             32568..35753
                     /gene="carB"
                     /locus_tag="IV247_001046"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAP8777131.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            35807..36583
                     /locus_tag="IV247_001047"
     CDS             35807..36583
                     /locus_tag="IV247_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAP8777132.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            36580..37512
                     /locus_tag="IV247_001048"
     CDS             36580..37512
                     /locus_tag="IV247_001048"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAP8777133.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            37509..38225
                     /gene="pyrF"
                     /locus_tag="IV247_001049"
     CDS             37509..38225
                     /gene="pyrF"
                     /locus_tag="IV247_001049"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAP8777134.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            38232..38861
                     /locus_tag="IV247_001050"
     CDS             38232..38861
                     /locus_tag="IV247_001050"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAP8777135.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            38849..39163
                     /locus_tag="IV247_001051"
     CDS             38849..39163
                     /locus_tag="IV247_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777136.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            39201..39842
                     /locus_tag="IV247_001052"
     CDS             39201..39842
                     /locus_tag="IV247_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8777137.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            39873..40577
                     /locus_tag="IV247_001053"
     CDS             39873..40577
                     /locus_tag="IV247_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAP8777138.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            40574..41476
                     /locus_tag="IV247_001054"
     CDS             40574..41476
                     /locus_tag="IV247_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8777139.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
BASE COUNT        13411 a         7337 c         9076 g        11747 t
ORIGIN      
        1 agatcacaca tgtatatgct ggatagtctt ataaaaaatc atgtaataca gcttggtaca
       61 tgatttttat ttgtagctgt tttttacata gacacaaaaa agaaaccacg cttgtagacg
      121 tggtttcttt tttactatct gtttccagaa gctgaggtgc tgctggatgg tgtatggtca
      181 tttgcagcat ccgctccgct agttgctggc tcactggaag aagcaggagg agcagatgaa
      241 gacggtgtcg agctttcttc ggatgaagaa gaaggaaccg ttgttgaaga cgctggctcg
      301 cttgtttctg ctgtcgtgct ttcttttgag gactcagcag ttgattcgct ttgactagta
      361 gagctagagc tttgagtcgt tgtctctgtt gttgagcttc caggagtctg gacatagctg
      421 gaagatggaa tagttgttga tggtgtaatt gcattcgatc tagctgtata ttgatctttg
      481 tagtacagtt caccgcccac acgaattaat ccgcttggca tctcccagtc tgtatttgtc
      541 acgttagctg aaacatactg cataagctcg cgataaacat cagaggctac gtgtgacgat
      601 tctgaagtga caggtgtcat ttttttattg taacctgtcc acacggaaat cgaataattt
      661 ggcgtatagc ccgcaaatag gatatctgga tacaccccag aagaaatacc aagttttgca
      721 tactcgtcat ctgtgtaatt ggatgttccg gtcttaccgg cttgatataa cccagcgatt
      781 tgcgcattcg taccagttcc ttcagtgatc gtatctttta aaatgtctgt gatcatataa
      841 gctgtttctg gagacatggc tgtttttcca tctggctcat aagtttcttc tgttccgtct
      901 tggaagacga ttttattaac atattgaggt ttgtaataag tccctccatt ggcgaaagcc
      961 gcataggctg ctgccatttt taaggaagaa gctccgtatt ttgtcccatc ttgttcttcc
     1021 gtattactag agatcgcatt cgattgatgg atcgtactgt attcgatccc aagatttttt
     1081 aagaaagaag ccactttatc tgaaccgact tcattgaata atttgactgc tggaacatta
     1141 cgggataagt aaagtgcttg gcgtaaagta atggtgccca tatattgatt atcccagttt
     1201 cctacaggag tcgaagtccc ttcatagtta tacggtgcgt cagtaatagt ttttccagta
     1261 gaatatttca aatattcaaa agctggtccg taatctgtga caggtttcat cgtagaacca
     1321 aaatcacggg aagtattcac tgctaagttg ttgcctaaag ttacgtcttc tgcgatgtga
     1381 cgtccgccaa tctgggcttt tactttcccg gtattggtgt cgataagggt ggaggcaact
     1441 tgcatctcgt cgtctgggta agagacatac tgatccgtgt tgacgatatc atatagtttc
     1501 ttttgggcgt ccagatccaa attcgtataa atctctaaac cgtctgtgta gacattctta
     1561 tctgtttttt cttgtacttc attgatgact tctttgacat aattatcgac gatcttagta
     1621 ttgtcatcgg attgagttag ttcttgcaga ccatccgtta ccggtacatt aacagcttga
     1681 tcatattctg tttgagagat tttttcgttt tgaagcatcg tatacaaaac agtatctcgt
     1741 cttttcttcg cctgatctgg ataaacataa ggatcatagg cggaaggggc ttgaggcatc
     1801 ccagcaagca atgctgtttg cggtaaagaa agttctgata atttcttacc aaagtacatc
     1861 tcagaagctg tctccatccc gtacaagccg tttgacatat acactttatt tacgtagtag
     1921 gtcaagatct cttgtttcga ttttttttgt tccaatctta cagccatcca cgcttcttgg
     1981 gccttgcgtt tcagagtctg atcctcggca ctggtggaga agaaggaaag tttgatcaat
     2041 tgctgtgtca aagtacttcc gccttgcagt ccgccagagg taaagttaga gagggcagaa
     2101 ccgatgatcc gaatagggtc aaccccgatg tgtttataaa aacggcgatc ttctaccgac
     2161 acgatcgcat cttctaatgt tttcggtaat tcattcgcag aaattttttc acgcttttct
     2221 gcgcctaaat cttcgaatag ttcgccatct tgtgtatata gtttggaaga tacggtagcg
     2281 tctaattttt tatccgtcaa ttcgggcgca tcttttgcgt aataccagaa aagtcctaca
     2341 cctgctaaaa agaggataca gaaaaatgag agtattccta aaaggatctt gataaataat
     2401 ccttttttat gtgttccaga ggactttcca gaacctttgc cagaattctt cttcacactc
     2461 tttttaggag taggctgttt ctgtcgtcta gagcttctag tttgttcatt tgccattctt
     2521 gctttctcct tcaatagata atgttgataa atagaagtca accgcgtcca aataaggaat
     2581 ccgcggagaa ataccagttt tgatctctat tcctagttct tcgatcaaag ataaaggtag
     2641 tgatttgcgt cctttttcct tttgggcgtc ccagtaagca atcaaatgaa cactattcaa
     2701 ataaaaacag cgcttcaaag acgaaaacca taacaagaca aagcagaccc cattttgttc
     2761 gaggcatgct ttcatatgtt cgatttgatg ggcatggaag tttttaaagg gaaaagatgt
     2821 tttgttcttg gtttcttttg cttcaaagtc caagtaaaag cctcgataga cgccattata
     2881 gtctgtagtt gaggcttggg taaagtaggc ttccttgatc acagcagcac ttctcttagg
     2941 atagtctact ttcacgattt ggataggggt aggcttttta tggataaccg ccaacccgcg
     3001 ggaaagataa tatttattac tttcattgat tgcttcttca aagcgcatcc cccgattgcc
     3061 gaattcagtg atctgatttc ttttttcttg aattttttcg acgggagagg cattcttata
     3121 ataaggttgg ccatttggat agcgcaatac catctttaat cacactccta ggccacatta
     3181 tagcaaaaaa gcggatagaa agaaaaggaa tctgagatgg ataagtcaaa agtaatctat
     3241 gtgtctgggt atcgaagttt cgaactcggt gtttttaaag aaaatgatcc taaaatccaa
     3301 gttgttaaaa atgttttaaa aaaagaactc catcagttag cagaagaagg gctggaatgg
     3361 gtacttgtca gtgggaattt gggcgtcgaa ctatgggctg tgcaaatagt agcggaattg
     3421 aaagaagaat atccggaact atctcttggt attttgtatc ctttttctga ttttggtaat
     3481 aactggaatg aagggaacca agaaaaaaaa caattagcag aacaattagc agattatgtc
     3541 gaagctgtta gccatcaacc ctaccaatca cctagtcagc taaagaatca cactcgtttt
     3601 atacttgagc atacagtggg ctgtctgctg atctatgatg aagaattccc tggaaaaacg
     3661 aagtattttt ttgaagatgc acagaattac caagcagatc atccctacga aattcggctg
     3721 atcagtatgg acgatcttca aaattttagt gaataatcga tattgtgttt gatttatcaa
     3781 cttttttcct atacaatgaa aaaggtagaa atgttattac gtaaatgaaa actagaggtg
     3841 taaatatggc gaatttggtt tatagtccta aagacatttt acaacaagaa ttcaaaacaa
     3901 aaatgcgcgg ctatgatccc gttgaagttg atgaattctt agacaatatc atcaaagatt
     3961 atgaaactta cagcaaagaa ctccttgctt tacaagaaga aaacgatcga ttgagtgcaa
     4021 aagtagctca attatctaaa actcaaggag cagctcaaac acgcgtgcaa caaacagagg
     4081 caccaaagag cgcagcagtg accaattttg atatcttaaa acgcttgtca aatcttgaac
     4141 gtgaagtatt tggtaaaaag cttgatcaac aagcaagtgc agtcaaacca gcacaaccta
     4201 atccaaataa ctacacaaat gcagacacaa gcttagatga taatgaaaaa acacgtcaat
     4261 tttaattttt gattaatgaa tttgcggttt tcgggtaatc gcggtctgac ttgatcaggc
     4321 tgaggaaagt ccatgctcgc acaggctgtg atgcctgtag tgttcgtgct tagcgaaacc
     4381 ataagctaag gtactctttg gagtaacggc aggaaaacaa gctaaggctt cggctatgct
     4441 taagtatcct tgaaagtgcc acagtgacga agcggctggg gaaacccatc cggtggaacg
     4501 cggtaaaccc ctcgagcgag caacccaaac aatggtaggg gcgctttttg tctggaagaa
     4561 gaacaggaca aaaaggcagt ataactgcag atagatggtt accgctttgt tttttctccc
     4621 tggaaaagca aagtacagaa catggcttat agaaaaccgc aaacatcagg gaattaggtc
     4681 gtgacaaaag taatgcgtta taagaaacaa gaaaaacatt tgaaagctat ttttcaatac
     4741 ttcagatgaa tcgttcttat tacaaaacaa acttttgtat caccggttat tcggaatttg
     4801 agtaaaagtg tggggtagga ctgatgtccc acctcctttt tttataaata aggagattgt
     4861 gtatggaaaa aaacaggaag ttcaacctcg tcgcaactgc ggcaagcggg ctagaagcac
     4921 tggttggtaa agaattaaga gatcttggaa tcgaatgcca agttgagaac ggacgtgcac
     4981 gttttgaagg aacaatggaa acgatcgcta cagctaattt atggttacgt acagcagatc
     5041 gagtaaaaat cgtggttggg gaattcgatg cgtatacgtt tgatgaattg tttgaaaacg
     5101 taaaagcatt gccttgggaa gattttcttc cactagatgc agcttttcct gtagctggaa
     5161 aatcgatcaa gtcgaaacta tatagcacac ctgattgcca agcgatcaca aaaaaagcaa
     5221 tcgttgaacg tctccgtacc tattatcacc gtccagcttc tgtaccactg acggaaactg
     5281 gcgcacattt tcagttggaa gtagctttgc tgaaagacca tgtgatggta actctggata
     5341 cgacaggacc aagtttattc aaacgtggtt atcgcttaga aaaaggtgga gcaccattga
     5401 aagaaaacat ggcagcagca cttattatgt tgacaaattg gcgaaaagac cgtccatttt
     5461 atgatcctgt atgtggttca ggtacgcttt gtattgaagc agccttaata ggccataata
     5521 tcgcaccagg attcaatcgt gagtttgctt gcgaatcttg ggattggttc agtcaagaaa
     5581 tcatggaaaa tgttcgatca gcagccgaag aaaaagcaga ttatgatatt gaattagata
     5641 ttactggttc agatatcaat ggacggatga ttgaaattgc caaagccaat gcagaagaaa
     5701 tcggattagg ctcttcgatt acctttaagc aacaagcagt caaagacttc aaaacggata
     5761 aagaatacgg ggtcatcgtg gctaatccgc cttacggaga acgtctagga gaagaagaaa
     5821 ctgttcggaa attatataaa gaaatgggcg atgtattccg tccattaaaa acatggagta
     5881 aatatatctt gactagcgac ttagcgtttg aggaatttta cggacaaaaa gcaaccaaaa
     5941 aaagaaaatt gtacaatggt gcattgcgta cggatctatt ccagtattgg ggaactcgtc
     6001 cgcctagaaa aaaagagaaa gacgaataat cttactaatt gattcattca attggatgtt
     6061 tggaggaata gggaatgaaa gaaaaagagt ttttacaaga agtaaaagaa atcaatcttc
     6121 tacaacaagc tttaggaatc ctcgattggg atacacaaac aggtatgcct gaaaaagcta
     6181 gtgcataccg aagtgaagta gacagctatc tttacagcat ttatttttca aaaaaaatcg
     6241 gtccaaaaat caaagaagcc attcgctatt tttctgaaca tccggaagaa ttgtcggaag
     6301 ttggaactgc cgtttttgcc aaagtaaaag aagagtatga tttggaacat cgcgtaccgg
     6361 aagatcttat gagaaaatat acagctgccg tttcttcagc acacggacaa tggcaaaaag
     6421 ctagagaaac acaacaattt tctgattttc aacacgcatt gaatcaaaat attgaattaa
     6481 caaaacagtt gataccttac tggagaaaag atgaaaagac gaattatgat gttttgctga
     6541 atcaatacga acccgggatg actgtcgaaa tccttgataa ggtatttgat cagcttaaag
     6601 aaggaattct ttcgatccgc cgtacgctcc aagaaaaagg gactgaacct gaaacagact
     6661 ttttaaatcg gagaatgacg aaaaaacaac agaagagatt tgtagtgaaa gtaatcgaac
     6721 aacttggtta tgattttagt cgtggtcgtt tagatgacac tgtccaccca tttatgactg
     6781 gaattaatcc gaatgatgca cgaatcacga cacgatggaa cgaaacagat ttcaaaatgg
     6841 ctgtttttgg agtgatccac gaggctggtc atggtatgta tgaacaagac atcgatgata
     6901 aatatgcgta tacaccggtt tatgaaggta catcaatggg tatccatgaa tcacaatcgt
     6961 tgtttaacga gatcattatt ggaagtagtc gcagcttctg gaaaaagcaa tatcctttct
     7021 ttcaagaatg tgcagaagga acattcgatg atattccttt tgacaaattt tatcgttcat
     7081 tgaaccatac aaaatcaagt ttggttcgta tcgaggcaga cagtttgact taccctctcc
     7141 atatcatcat tcgttatgaa atcgaaaaaa tgattttcaa tgaaggggta gatgttgctg
     7201 aactacctga aatatggaat caaaaatatg aagagtatct gggaatacga ccaacaaatg
     7261 atgtggaagg catcctacaa gacgttcatt ggtcaggtgc aagctttggt tattttccct
     7321 cttatgcatt agggtacatg tatgcggcac aattacttca ttctttgcag caagaaatag
     7381 atgtggaaaa tgttctagcg tcagacgatt attctccaat caaaaactgg atgtcagagc
     7441 atatccatca atatggtgca tctagaaaac caaatcaatt aatcatggat gcaacacatg
     7501 aaccgcttaa tccgcaatac ctgatcgact atatgaaaga cctgtatttt aatgtatatc
     7561 aggtaaaata aaatacaatt tacaaagata gctgaaattc atctaatcta tagatgtttt
     7621 cagctattta cttaagaaag aagtggaacg aatggataaa ttacaagtga tcgctgatta
     7681 tagtatggac caattagcgc atgatcaaac aggacatggg agcgatcata ccaaaagagt
     7741 tgtcaagtta gctgaaaaaa ttttggagac ggaaccgcag gcagaccgct ttgtgacgct
     7801 tgcagcagct tacctacatg atacgattga tgataaagtc gttaaaaatg agaacgaggc
     7861 aaagcaacag cttcgtgtat tccttcgctc tttgccaata gtagaggaac agatctcgat
     7921 gattttttcc attatcgaaa atatgtcctt ttctaaaaat ctctctgaag cggccgaact
     7981 atctctggaa ggtaagatcg ttcaagacgc tgatcgtatc gaagcattag gagcaatcgg
     8041 cattttgcgt actgcttatt tcggcggtgg acatgggcac ccgatttttg atcctgaact
     8101 gtatccgcaa acattcaagg acaaagaaaa ttaccgaaaa ggcacgacag tgatcaatca
     8161 tttttatgaa aaactgttct tgctgtctga taaaatgaat actgattacg gatatgaaga
     8221 agccaaacga agagaaacct tcatgcgtga attcttagaa gaattttttc tggaatggtc
     8281 tattgacgat gaaaatttta ctccagacaa ttggatcaaa aataacaaaa aatgatggag
     8341 gttgtgacag aagtggtcag ctccaagata tttcggatca ttttccgtaa aagttacttc
     8401 tggaacactg tttaccaaga ggtcgtgagc ctgccgttca gctccgagtc acaaggaaca
     8461 atttcaaaaa atagcttctc gtgtttgatg aatattgtaa cttgtgattc ggaattggca
     8521 ggagaacacc gtttattcgg aagaatagga ctgtgacaag acttttgtca cagtcctttt
     8581 ttcgaattca acaagtaaaa cttttaagaa atattcagag agtaaaatta aaaattgctg
     8641 ataaacgaag atggaaattt tatgtatttt tattggtcta tgcaataaat aaattaaata
     8701 ttcagaatac aattgatttt aacgaaaggg cttctatcaa tggttgtgtt gtatttatct
     8761 gcccaggtag agtaaaatag atcattatct aaaaggtaat tgttcatact atataagtca
     8821 tttgaaagta aaaagatact gtaaataaaa aatgtggtta aataattctc ataatcagtg
     8881 taagcgttat caaaaatata gcatacttgc tttgtagatg aatgacacga tatatgtttg
     8941 gtatgttctg caagacagat gaatatggag gtggagacaa tgtcttccaa agaactaaca
     9001 atgtatttag agaaagaatt ggatcaactg aaagaaaagg ggttatacaa tacgatcgac
     9061 atactagaaa gtgaaaatgg tgcttgtatc atcgtagacg gaaaaaagat gatcaatcta
     9121 gcatccaaca attatcttgg atttgccaat cgcgaagaat tgaaaaaagc atgtattgaa
     9181 gcaaccgaaa cttatggtgt aggagcagga gcagtccgaa cgatcaatgg ttcgctgaaa
     9241 atccatcagc aattagaaga aaaaatcgca gaattcaaag ggacagaagc agcaatcgcc
     9301 tttcaatctg gattcaactg caatatgggc gctatctctg cagtaatgac aaaagaagat
     9361 gctatcttat cggatgaatt aaaccatgca tctattattg acggttgccg cttatccgga
     9421 gcaaaaatca tccgaattaa acatcaagat atgaaagacc ttgagaaaaa agcaaaggaa
     9481 gcgatagaaa gtaagaaata taaaaaaatc atgtatatca cagatggtgt tttctccatg
     9541 gatggagaca ttgcacgatt gccggaaatt attccgattg ttgaaaaata tggactgatc
     9601 acctacgtcg atgatgcgca cggctcaggc gtaactggaa aaggtgcagg aaccgtcaag
     9661 cattttggat taagcgacaa aatcgatatg caaatgggca cgttatcgaa agctataggt
     9721 gttgttgggg gttatgtagc tggatcgaaa acactaatcg actggttgaa agctagaagt
     9781 cggccattcc tattttctac ttcgctaact ccaggtgcag ctgcagctgc attggcttcg
     9841 attactttga tgcaagaaca tccggaactt gtcgaaaaag tttgggaaaa tgctaattat
     9901 tttaaagaag aattgaaaaa agtaggctat aacatcggta tgtctgaaac acctattact
     9961 ccagtcattt tgggagacga aaaagtgact cagactttct ctaagaagct gatcgaacat
    10021 gggatttatg ctaaaccaat cgtttatcca actgtgcctc ttgggacagg caggatcaga
    10081 aatatgccga ctgcggaaca tacgagggaa atgcttgatg aagcagtagc agtttatcaa
    10141 aaaataggaa aagaaatgga aattatttaa ctaaatttag ttttttcgtc tatttaaaat
    10201 tagagaacat gttcacgcat atattgttaa tcatctgaag atagtagtaa aaaagatcag
    10261 ggaggatcat catgaaaaaa gtattggtga caggatgttt aggacaaatt ggttcagagt
    10321 tgatcacacg gctacgtcaa gaactaggca gtggaaatgt cattgcgaca gatatacgca
    10381 tgcctgaagt cattgagaaa aacgatatct ttgagatcct agatgtaatg gattacgaaa
    10441 aaatgcgcag catagcagaa caatatcaag tggacacatt gattcatttg gctgctcttt
    10501 tgtcagctgt tgcagaagca aaaccacaat tagcctggaa tttgaacgtg actgggctgg
    10561 tcaatgcatt ggaagtcgct agagaattcc agctgaaatt ttttacgcca agttcaatcg
    10621 gcgcgtttgg tccaaatact ccaaaagatc ataccccaca agatactctt cagcgtccta
    10681 caaccatgta tggggtaacg aaggtagctg gagaactact atgtgattat tattatgtga
    10741 aatatggcgt agatactcgt ggtgtccgtt tccctggatt gatttcttat gcaaccttgc
    10801 caggaggcgg tacgacagac tacgcagtcg atatttatta ttcagcaatc aaagaaggaa
    10861 agtacactag tttcattgat caaggtacat caatggatat gatgtatatg ccagatgcga
    10921 tcgatgccat tataaaactc atgaatgcag atcctgatcg attggttcat cgaaatgcct
    10981 tcaatatatc ggcgatgtct tttgacccgg aagagatcaa agcatctatt caaaaaatca
    11041 taccagattt tgaaatggat tatgatgtgg atcctgtacg tcagtcaata gcagaatctt
    11101 ggccaaattc actagatgtt tcttgtgcgc aagaggaatg gggcttttcg ccgaaatacg
    11161 acttagatgt gatgacaaag gatatgttgg aaaaactgaa agaaaaattg gtagagacgg
    11221 tataaaacaa gaaccacatc caaaaagttt tttatacttt tgggattggc tatggtatga
    11281 aaggggacaa gggatggaag aatatcaagc cacgccagta aaagaagtaa aaatagaaaa
    11341 tgaattaaaa cgtacgatgg gattttttac tgctttatcg actgttatgg gcacagtgat
    11401 cggtgcaggg gtatttttta aagccgcaag cgtagcagaa gttacaggat ccgccagttt
    11461 gcatatgttt tcttggtttc tcggtggtat gatttctgtc tgtgcaggac taacaggagc
    11521 tgaactggca gctgctattc cagaaacagg agggatgatt aaatatatcg aacggattta
    11581 cggaaataca gcagcttttt tattgggctg ggcgcaagtc gtgatttatt ttccagcaaa
    11641 tgtagcagcc ttgtcgatca ttttcggtac acagttcgtc aatctatttg gtctttccca
    11701 gtcaatgatt gttcccgtag ctgttacagc tgctgtatcg atcctgttga tcaatttttt
    11761 aggttctaaa gctggagggg cttttcagtc gattacgctt gtctgcaagc ttattccttt
    11821 gtttgtgatc gtcattttcg gtttgttccg acaagaagga gtagacttcc aactgtttcc
    11881 gatccaagca ggagaaaatc tttcattttt ctcagccttg ggagcgggct tgctagcaac
    11941 gatgttcgca tacgacggtt ggatacacgt aggaaatatt tcgggagaat tgaaaaagcc
    12001 ggcgaaagat ttgccaaaag caatttcttt aggaatcatc ggaatcatga tcgtttattt
    12061 attagtaaat gcggtgttct taagaaccgc atctatcgac ggagtcgcag gaaacagcaa
    12121 tgcggcaagt gacgttgcaa aaatgatttt tggcggcttc ggtggaagac tggtcacagt
    12181 tggtatcttg atttctgttt atggaaccat caatggctat acccttactg gaatgcgtct
    12241 cccttatgtt atggcaaaag aaaacaacct gccttttagc aagctctttg ctaaacttca
    12301 tgataaaact aaagttcctg tagccgcagg tatcttagag ctagtcatcg ctattggcat
    12361 gatgatgatc ggtggttttg atacactaac ggatatgctg atctttgtca tttggatttt
    12421 ttatacgatg gtctttgttg gtgtcattct tcttcgtaaa aaagaacctg atttgtttcg
    12481 accatacaaa gttccaatgt atccgtttat tccactagta gcaatcattg gagggacttt
    12541 tatcgtatca agcacgctta tcactcagac ctttttagct tcgatgggga tagtattgac
    12601 acttgctggt atcccgattt atctctattt aaaacgtaga tagctaaaac attcgaattt
    12661 tattagaact gcacttctct gccaaacaaa atattttatt taaatgagga gtacgtggaa
    12721 gaaatgtcaa aagtactcgt taattttgat cacgtaaatg tcgttttgtc tacgatgaaa
    12781 tagcaaagta aaagacagaa atcgggctca accgatttct gtcttttact ttgatttgcg
    12841 taactgaact attcggcgta cgccgcgata aatatttaat aaaaccaagc aaccagaaat
    12901 gaaaatggga agccaaatca gatagacagg gtagccaagc actttagaca aagcatgtgg
    12961 agtcatttta tccatttcaa gaagtgcttg ctgccaagtc tgaagattga agactagcat
    13021 gaaacccgaa ataatcagga gaataaatcc taaataatga attgtagttc tttttcttac
    13081 ataagcgatc gtagcataaa ggaacagtaa aaaaataggt acgccaaaaa caaagtacat
    13141 attcagcatc tcgacacccc ctttgtgttt atcttacttt actttgaaag aaatgagaaa
    13201 taatagcctc gttacttgct tgaaacagca aaggactgct aagataagag agatacaaag
    13261 aatcaaggag gagtcatatg gcaagttata tcgaaccgat tcgttttgcg ctgattgttt
    13321 ttccattttt agccttagcg atctcatctg ttttttttat ttatgaatat cgaaagtatg
    13381 gaacatttct agtaacaagg gcagttattt tgtattcttt cgttttttat ttattgtgtg
    13441 cgtatttttt ggtgatctta ccacttccgg caaaggagac ggttgcccaa atgacagggc
    13501 caaagatgga gctgcattta tttgcttcat ggcaacattt cagagacaac acggtattgg
    13561 tcttgtctga acctagtacc tacctgccgg caatgaagca gagtgtattt ttggaaccag
    13621 cttttaatgt tttattactc ataccatttg gcatttattt aagatattat ttccggcttt
    13681 cttttatgaa aacgatgatt gctagtttct gcctttctct attttttgaa ttgacgcagc
    13741 taagtggact ttattttatc tacccaagac cataccgctt atttgatgtg aatgatttag
    13801 tgacaaatac attgggtgga atcattggtt ttgtcgttac gccgttattt acatttatgc
    13861 ttcctacaag aagacgaatg gatgaacttt cttatgaaaa aggaaagact gtctcattga
    13921 ctcgacgctt ggtcgcttat ttgatcgact ggacattttt atctcttatt aatttggtag
    13981 cggcgatttt ctttaaaatt gcgttcgatt caggggcagt tacttctcat atctggtggc
    14041 ttttattaga agtaggggta tactttatga ttgtaccgtt tataaccaat ggatggacac
    14101 ttggcaaaaa aattgtgcgt atccgtttgg tggaagaaga ccgcgaacga atttctttaa
    14161 aagcattgat gatccgctac gggtatctgt actttctttt ttatggttta agctggttgg
    14221 ggcagttgct tcagcaaatc atgaattcga caacgaatac ggtgttgcag atgagtgcag
    14281 tattcatttc tctcggtgtc ttgttactac aaggtatttt tattttaaat atcttgctat
    14341 catttttgag aaaaaaacgg attctgtttt atgaaaaagc tagtcacaca tatactgtta
    14401 gcacgattgg ccaaacaaag gatgatgatt ctcgaaatta gctgagcaaa aacgctttgt
    14461 ctgtgttata atgccaaagt cgtgataatt taaagagaag aggtgcacga atgaataatt
    14521 ttgatatatt ccgcttcaaa ccgtttatca atgaagcgtt aaccgcaaag ggcttcaaac
    14581 agcccacaga ggtccaggaa cgtttgattc ctttaattgc aaaaggaaaa aatatcattg
    14641 ggcaatctca aactggtaca ggaaaaacac atacgttttt gttgcctttg tttgaaaaaa
    14701 tcaatgcaga aaaagaggaa gtacaagttg ttattacagc tccaagtagg gaactagctg
    14761 cacagatcta tgaagcagct acacaaatcg ctaaattttc tacaccggag atccgtgtct
    14821 cgaattttgt cggcggtaca gacaaacagc gtcaattaaa tcgtctgcat caccaacagc
    14881 cacaggttgt agtaggcacg cctggtagaa tattagacat gatgaatgaa caagcgctga
    14941 agattcacac cgcatttgca tttgttgtcg atgaagcgga tatgacattg gatatgggtt
    15001 ttttaaatga agtagaccaa attgcaagtc gattaccaga aaagctgcag tttttagttt
    15061 tttctgccac tattccagaa aaacttcgtc cattcttacg aaaatacatg gaaaatccaa
    15121 tcattgaaga aatcaaacca aaagcagtta tttctgaaga cattgaaaat tggctgattt
    15181 caacaaaagg taaagatgag aatcaaatga tttatcaact gctgacaatt ggtcatcctt
    15241 atttagcaat cgtatttgcc aatacaaaac agcatgtgga tgaaattgcc gattacttga
    15301 aaaaccaagg cttgaaagta gcaaagattc atggagacat caccccaaga gagagaaaac
    15361 gagtgatgcg acaagtacag aatctggagt atcaatatgt tgtagcgact gatttagctg
    15421 ctagagggat cgatatcgaa ggtgtctctc atgttattaa tgcagaaatt ccagaagatc
    15481 tagacttctt tatccatcgt gtcggacgta ctggacgtaa tggcttaaaa ggaacggcca
    15541 ttacgttata ttcgccaaat gatgagcaag ccattgatgc gatagaaaaa ttgggcgtct
    15601 catttgaacc aaaagaaatc aaaaacgggg aaatcgtcaa aacatacgat cgtaatagac
    15661 gtgccaaacg agaaaaatca aaagatgtat tagatcccac gctgattggt cttgtgaaga
    15721 agaaaaagaa aaagatcaag cctggttata agaaaaaaat cgattgggca attgcagaaa
    15781 aaaataaaaa agaacgcaaa atcgaacgca gacagcaaag ccgtactgct cgtaaaaata
    15841 agaaaaattc acataaataa aaaacaagag gttgtgacaa aaggcgtgtc acaatctctt
    15901 gtttttaagc tagacttttt ttcattccga tacaaagtac ttcatcttca ataaaaggtt
    15961 ctgtgcaagt atgataccct tgtttttcat aaaaatctac agccgtcatt tcagctacta
    16021 cataactagt ctgacaacct tctgaaaccg cccgtttttc agcagcttca agcaactgac
    16081 cgcctaaccc ttttcctcgg tattcttttg ctacgcaaaa tcgttccggc ttgaagcaag
    16141 taggtgtttt cttttcataa cgcaaagtag ctactggtct tttttgatca agaaatagat
    16201 aataggtttg ttgcgggata tcttttccgt cgaattctag ttctgcagga atcccttgtt
    16261 ctaatacaaa aacttgatat ctcaaaaagc agctagctgc gcgtatccat tcatccgctc
    16321 caactatttt tttcatcata ttcctccttg tcatgaatag cagagatgta ttgattcctt
    16381 tattcttatc gtaccaaaaa tcgtggagta tgaccaaaag gaaatgaaaa taaaaattga
    16441 gatattgaca gtgatcaata gaatgcgtat actaaataag tacgaaaagg atatgatttg
    16501 ttttttaaga ctcacagaaa attctttctt ggctgaaaaa agaatagtca ctaatcaaat
    16561 tgctcccttt ttctattttg gtagaaatac cgacgcagct cagcgttatc gagtttaaga
    16621 ggtaatgatt acacatcatt gcaatcaagg tggtaccgcg agttttcgtc cttggctgtg
    16681 atggtgtttt tttgtattta atttatagaa agcaggtaat gacatgaaac aattaagtag
    16741 tgcacaagtt cgacaaatgt ttcttgattt cttccaatct aaagaacata cgattgaacc
    16801 tagtgcgtct ttgatccccg tgaacgatcc aaccttattg tggatcaact ctggtgttgc
    16861 aacattgaaa aaatattttg atggttcagt tgtgccagaa aatccaagaa tcacaaatgc
    16921 ccaaaaatct attcgaacaa atgatatcga aaatgtcggt aaaacagcac ggcaccatac
    16981 gatgtttgag atgctgggta atttttcgat tggtgactat ttcaaaaatg aagccatcca
    17041 ctgggcatgg gaatttttga ctgatccaaa atggatggca ttcgatccag aaaaacttta
    17101 tgtaacagtt tatccaaaag atacagaagc aaaacgcatc tggcatgaag aagttggcct
    17161 gccactagat cacattattg atgtagaaga caacttctgg gatatcggtg caggcccatg
    17221 tggtccggat acggaaattt tctatgaccg tggagaagct tataacgatt tagcagaaga
    17281 cgatcctgaa aactatccag gaggagaaaa tgaacgttat ttagaaatct ggaacttagt
    17341 attttcagaa ttcaaccata agcctgataa cacatttgaa ccacttcctc ataaaaacat
    17401 tgatacgggg atgggattgg aacgcatggt ctcaatcgtt caagatgcgt caacgaactt
    17461 tgagacagac ttgtttatgc ctattatcca cgccgtagaa aagctaagca aacaagtaaa
    17521 atatggtgaa aacccagtaa cagatatttc ttacaaagta atcgcagatc atattcgcgc
    17581 cctttcattt gctattggcg acggtgcatt gccatcgaat gaagggagag ggtatgtctt
    17641 acgccgtttg cttcgtcgtg ctgtcatgca tgggaaaaaa ttaggtattg aacaagcatt
    17701 cttatatcgt ttagtccctg ttgttgggga aatcatggta agttattatc ctgaagtact
    17761 tcaacaaaaa gaatttattg aaaaagttat ccgtacagaa gaagaacgtt tcaatgaaac
    17821 aatcaatgaa ggtttggaaa tcttaaatca agtgatccaa gaagtaaaaa acgaaaacgg
    17881 gacgactcta gcaggaaaag acatcttcaa actatacgac acatatggct tcccggtaga
    17941 attaacggaa gaggtggcag aagatgaagg actgaaagtt gatcatgctg gttttgaaaa
    18001 agaaatggat gcacaacgcg aacgtgctcg ctctgcccgc agtactgaaa aatcaatggg
    18061 ggtacaatct gctttattga cagatatcaa agtagaaagt aaattcattg gatacacaga
    18121 acaagaatca gatagtcaat tattggtcat cgttaaaggt gagtctcttt tgaatgaagt
    18181 cgcagaaggt gaagctcaaa tgatctttga tcaaacacct ttctatgcag aaatgggtgg
    18241 gcaagtagcg gatcaaggat ggatcatgaa tgaagtaggc gaaatcgtag cagttgttac
    18301 agatgttcaa aaagcgccta atggtcaatt tatgcacacc gtagaagtaa aagcaccaat
    18361 gagtgaagga caaacctatc atctattgat cgatcaatcg atgcgcaatc ggatcatcaa
    18421 aaaccataca gcaacccatc tactgcacaa ggcattgaaa gaagtacttg gcgagcatgc
    18481 aaatcaagct ggttctttag tcgctcctgg acatttacga ttcgacttta cccactttgg
    18541 tcaaatcact tcagatgaat tagctgaaat ggaaagaatc gtcaacgaaa aaatctggga
    18601 agctatccca gttgaaacaa ttgaaacaga tatcgataca gccaaaaaca tgggtgcaat
    18661 ggctttgttt ggtgaaaaat acggacatga cgttcgagta gtaaatattg gcggctggtc
    18721 aatcgaactg tgcggaggca cacatgtagc aaatacagaa aacatcggaa tcttcaaaat
    18781 cgtttctgaa tcaggaattg gtgcaggcgt gcgacgaatc gaggctgtaa caagtaaaga
    18841 agcgtacgaa ctacttgaag aagaagaaaa acaactaaaa caagtagcaa caattgttaa
    18901 atcacctcaa ttgaaagaag ttgtttcaaa agcggaacaa ttgcaacagc aagtaagaga
    18961 gctacaaaag gaaaatgaac aattagctag caagttagcg aatcaacaag ctggtgatgt
    19021 attcaaagat ccagaaacga tcaatggaaa aacaatcatt gcagcacaag taaaagtgaa
    19081 agatatgaat cagctgcgtc aattagctga ccaatggaaa caaaaaaata tttcagatat
    19141 tcttgtttta gcaaacgaac aagaaggaaa agtcagtctg ctagcggcaa tgacgaaaga
    19201 atcaaatgaa gctggactaa aagctggcga cttgattaaa gcaatcgcac cgaaagtagg
    19261 cggtggcggt ggtggacgtc cagacatggc acaagctggc gggaaaaatc ctgctggtat
    19321 tccagatgca cttaaagctg cgaaagaatg ggttgcgcaa caatagggcg tttacgtttg
    19381 acagaaaaac aaaagttcaa acattgaacg aaacatgatt atgggggcga agacaaacaa
    19441 gtcttgccct catacttttt tcaattggat ggcgcatcta atcaaatggt cggattcgga
    19501 tggtctttag ttgacgaaag tgacagaaat ttttacgatt agtataaata aaaaggggtg
    19561 aaaaaatgaa tagtagaaat aagacctttc gactagtctt acgtgcgatc ttgttagcaa
    19621 tcatcatcgt acaagcgatg gttccttggc tggggtttat tccattagga ttcattagtt
    19681 taacaattat ccatatcaca gtaattattg ctgcagtagt attaggccca aaagatggca
    19741 tggtgattgg cttgttttgg ggaatcgcta caattgttcg tgcctatgcc atgccaacaa
    19801 cgccatttga tacattagtg ttcacgaatc cgattatctc ggtagttccc agagtacttg
    19861 ttggcttagt agcaggcttg gttttccact ggatttatca aaaaaataaa tcaataacga
    19921 tcagctcagc ctttgcaggg gtactgggtt cattggtcaa tacagtctta gtgttaggat
    19981 tcatgggatt attttataca ggtgcaacgg cagatgccta tggtgtagac tctgctcttt
    20041 tatttaaaac cttagcaggg gttgcagcaa tcaatgggat accagaagca ataggtgcgg
    20101 gaatcataac accattacta gcaaaagcgt tatttgcagc aacaccgttg aaaccagaat
    20161 aatgaccatc ttttaatagt aataggacgc acgatagata gaaaaaggaa gagaggagag
    20221 gaaaacaatc gtcctctcca tctttttacc tgagcaacaa gcaaactaga aggggtaaaa
    20281 aacttggcta agaaacaaga agaaaaaaag aaacaaatac cgcaacagcg gacaaaaaaa
    20341 ggagtactag cgggtatctt aggattggtt acgaatctga tcttgtttgt ctctaagcta
    20401 atgatcgggc ttttttcagg aagtgtctcc attatggctg atgcagtgaa cagtctatca
    20461 gatactgctt catccatatt gactttgatc ggatttaaaa ttgctgcaaa gccagcagat
    20521 caggaacatc cttatggaca cgaacgcttc gaatatatca gtgggttatt tgtttcgatc
    20581 atcattactt atgtaggatt tcaattttta gagtcttctg ctgaaaaaat catccatcca
    20641 gaaaatgtct ctttaacacc catcgtcttt tttgtcctaa tcatttccat ctttttgaaa
    20701 tttgcacaag gcaaaatgta tcaggttatt gcgcaatcca ttgattctga aacattgaga
    20761 gctacagcaa cagatagtta taatgatgtc tttactacac tggctgtatt gctttcagca
    20821 gcaatcgaat gggtaactgg atggcgtatt gatggatata taggttttgc tttagcatta
    20881 tatatccttt atagtggatt gacgatgctt cgaaattttg tatacgaact attaggaagt
    20941 cgcccaacgc cagcagagat tgaagaaatg gaagagcatt taaatgcttg cgatgatata
    21001 cttggctatc atgatttatt ggtgcataac tatggaccca ataaacgctt tgcatcagtc
    21061 cacatcgaag tagatgaatc aatgaatttg aatgtggctc ataagattat tgatctgata
    21121 gaaaaagact ttaaaaagtc tttggatgtg gaacttgtct gtcacttgga tccaatgcct
    21181 gttgataacg aaaaatacta taatatcttg cggcagttaa agcaaattct tcttgaaaca
    21241 ggtacagggc ttaatattca tgattttaga gtaattcatc aaggaaaaac gctgcaattt
    21301 gatgtagtgg tccctgagaa attccagttt gttgatacag aactagaaag aatcttacgt
    21361 agtcaaatag aagaaaaaat cggagattac caattggaaa ttacgtttga tcacaattat
    21421 cttttatatt aaatgagaaa ctataaaaag actccttaaa gtttttaatt taaagagtct
    21481 ttttttcttt taattgttta tggagagcct gacgtgcaag attatctgct cctttatttt
    21541 tgttttcggg caaccattgc aggatcaata aagaaaattc ggatagaagt tcttggattc
    21601 tttgaaggta gggaaggaag agctcgttct tcgttgcgtt tttgtcgacc gtagaaacta
    21661 gtgttttgct gtctgaaaaa cagaaaatcg tttcattctg taagtcgttt ttctttagaa
    21721 attctaaagt ttttaaaaag acttcgaact ctgcttgatg gtttgtccct atagaaagcg
    21781 ggatagatag ctgttcttgt ttaccttgat agacaatcaa gattccacct ccactaggac
    21841 cagggttgcc ttttgtagag gcatcaatgt atacttttaa cataaaaaga gattctcctt
    21901 tttgttcaaa ctaatgttat ggtaagatta tatcattact gaatcggtga gaggttagag
    21961 aatgaaaaaa aatattcgat ggcagcctga actggcacat acgatcatct attggtcatt
    22021 gaccttgatc attctgtttt ttagtctgat tctttcttta gaaaacacag gtccttattg
    22081 gaaaagcaat tttgttttag gtatttttct tgtttttcta tttctaggtt tgcggcgttc
    22141 ttttatttta aaatcaaact ctgtgattat acgttatgct gctttttgga aagaccgaga
    22201 aatatctgtc ggacagattc gatctttgac gccaactaat actggggtgc aaatgatgtt
    22261 gcaagatgag aaagccccat ctgttttcat gatgcgaaaa aaagatcaag tagtcctttt
    22321 tgatcatttt gaaaaaaacg gacccaaatc agaatcaatt ccttatcctt atacgaaaaa
    22381 aagaaaaagc taattttaaa aatcagaacc taaaaaaacg aatttttaca ggaaaagaaa
    22441 agagtgagac gtacggttca tcccgtttag cgtctcactc tatttgttta tctattcatg
    22501 catactagta tcggtagtct gaacgttgcc tttcttgaca tgggcagctt gtgttcctcg
    22561 attcccttcg atgatctcaa attcgaccac ttgattttct tccaaagttt tgaatccctc
    22621 ttcttcaatc gcagtaaaat gtacaaaaat ttcttctgtg tcatcataac tgataaagcc
    22681 gtatcctttt ttgttgtcaa accattttac aattcctgtg gtcaaagcac tcacgacctt
    22741 tctaaagtct gatcatagcc ttcattataa agagtattta taagtacgtc aaataaatta
    22801 aaaacctgta attttatgtt ttttttctga ataaaaaaag gtataattgg catatcttaa
    22861 caaagaagat attttgttga ataaagttcg tgaaaagggg taatcaaatg aagaatgatc
    22921 tgcaaatttc acaagaaaca gagctagaac cgattgttga agtggctagt aaattagatc
    22981 ttaaggaaga cgatttggaa ctgtatggga aatataaggc gaaaatcaat ttttctggaa
    23041 tcaatcgatc aaaggataac gctgaggggc atttgatctt agttacttcc atcaatccaa
    23101 caccagctgg cgaaggaaag tcaacgatca ctgtagggtt aggagacgcg ctcaataaaa
    23161 tcaataaaaa atctgttatt gcattgcgag aaccctcact tggtcctgtg atgggaatca
    23221 aaggtggtgc tgcaggcgga ggatatgcac aggtattgcc tatggaagat atcaacttgc
    23281 attttactgg tgacatgcat gctatcacaa cagctaataa tgcattatct gctcttttag
    23341 ataatcatat tcatcaagga aatgaattga acatcgatgc ccgccgagtg atttggaaac
    23401 gggtagtcga tctcaatgat cgtgaactgc gtaaagtaat tgttggcttg ggtgggccaa
    23461 tccaaggtgt tccaagagaa gatgggtttg atatcacagt tgccagcgaa atcatggcta
    23521 tcttgtgtct tgcggcagac cttgaagatt taaaggctcg tcttgcaagg atcgttgtcg
    23581 gctatacatt tgaccgtcag cctgtgactg ctggagactt gaaagcagaa ggcgctctgg
    23641 cattactgct gaaagatgca atcaagccga atcttgtaca aacaatttac ggaacaccag
    23701 cttttgtcca cgggggaccg tttgccaata tcgctcatgg ctgcaatagt attttagcta
    23761 ccaagacagc attgagatta gcagattacg ttgtcacaga ggcaggattt ggtgcagatc
    23821 ttggcggaga gaaattttta gatatcaaag tacctaactt gaaaaaagcg ccggatgctg
    23881 tggtgattgt cgccactgtc cgtgcattga aaatgcatgg cggaatgaaa aaagatgaac
    23941 ttaaaaatga gaatcttgat gctttgaaag taggttttgc aaatttaaag cgccatatac
    24001 gtaatatgga acaatatcag ctacctgtga tcgtcgcaat caatgaattc gtaacagata
    24061 cagacaatga actcacactg ttggagcact tgtgtgaaaa tcaggggatt cttgccaaga
    24121 gagcgagtgt atgggcagat ggagcagaag gcggcgttga tttagcaaaa gctgtagtac
    24181 gattgattga tagaaaagaa gctaattaca agccattata ccgcctagaa gaaacgatac
    24241 aagaaaagac agaaacaatc gtcaagaaaa tttacggagg aaatggtgtt gttttcagca
    24301 aaaaagcgca aaaacaaata gaagagttta cgaagaatgg atgggacaaa ctgccgattt
    24361 gtatggcaaa aacacagtat tcattttctg atgatccttc actgctaggt gcaccggaag
    24421 atttcaccat cacgatccgc gaaatcatcc caaaattagg ggcaggattt ttagtagcct
    24481 tgactggaga tgttatgacg atgcctggtt tgcctaaaaa acctgctgca ttgaatatgg
    24541 acgtgacaaa tgacggagaa gtgttgggat tgttttaagc ttgattataa tctaccatcg
    24601 aaggagctgt attttgacta cagcttcttt ttttagacga tagggaatta attttgttta
    24661 cactaggatc attctttttc tttattgtat tttgagctat actaaagcat aataggaggg
    24721 tatgatgaaa acgacattta taagaaaaac cggtttttac ggtatgggta gctcgattca
    24781 gctaagaatc aacaatgaaa aacgcagtgt tctcagtcat gaacaaaaga taacgcttga
    24841 tctggaagtg ccctttacga tgcaggtcac cttttattgg ctaaaaagcc ccgtttatac
    24901 agtgaaagaa gcaaagcctg cttatgtgat cacaatgaat tacttgattc ttcaaatcta
    24961 tccgttttta tttctagcaa ctgggttggg ggccgttttc ctacaaaatt tatttttcag
    25021 tttttttgca gttattgtgt tgattgcgtt ctttttctat ataaaaaacc gggcatattt
    25081 gatcaaggag gaaaattatg gcaaactctg atcttttttt aatcagtttc aatcgaatcg
    25141 aaaaatggat gagagacgag atgggaaatg cacgaaatat gggatttacc gaacttgtca
    25201 gacgcctagc tcaaagaaaa caattaatga tcagaaaata cgaagatgat cttttacagc
    25261 ttgctcagtt aaggaatgcg atcgtccatg atcggatcgc agtcgatttt gtcattgcag
    25321 agccaaatga ttggacagtc aagcgaatcc agctgatcga acaagaactg attcagcctg
    25381 aaaccgtgct gcctcgattt gcaaaacgtg taacaggttt tgaacaagat cttccgttac
    25441 ttgaactgtt aaagatagta gcagaaaaaa ggtattcaca atttccgtta tacaataaag
    25501 gaaaatttgt agcattgatt actttacgta atatcgggtt ttggctagca aaagaaagcc
    25561 aaaaaggtcc ggttgattta acaaataaac gagcgttgga tttgatcatc caaaacggaa
    25621 agtacacaaa ttatcatttt gttccagcta aaacacatat ttacgaggtt gaagcgatgt
    25681 ttcgtgagca agggactttg gaagccgtac ttatcactaa agatgggaat cccgatggta
    25741 atctgctcgg gatcgtacga cctcgtgata tttataaaca aatagaaaag gattgatttt
    25801 ttgttaatta tttattggat attcagcgct ttgctagtag gagtggatca gttggtcaaa
    25861 tggtggatca ctgataatct agcgcttgga gagacgaaaa atcttatacc aggtattttg
    25921 tcactgaatc atattcgaaa cactggagcc gcatggagta tgctagaagg gaagatgtgg
    25981 ttctttacta tagtgacact cattgcagtc gttgtgattc tcacattgat gattaaaaat
    26041 cgtgaaaaag gtaatcgctg gttcatgatt ggattgagtt tgattctagc tggtgcgatt
    26101 ggcaatttta tcgaccgtat tcgtcttggc tatgtagtag atatgttcca gacagatttt
    26161 atgagctttc cgatttttaa cgtagcagat gtgacacttg tatgtggagt gatttgtatg
    26221 ttaatttata ttattttaga tgaaaaggat caaagaaaaa aatgacagaa cttaaaacga
    26281 cgattcacca tgaatctggt aggatcgata aagtattgac taatttattc accgattaca
    26341 gtcgttcgca aattcagctg tggctgaaaa atggagcggt ttctgtcaat ggacagccag
    26401 tcaaagcaaa ttacaaagta aaacaaggag acgagattac tgtcttgatc cccgaaccag
    26461 aaacattaga tgtggtccca gaaaatatcc cgttgacgat cgtttacgaa gatgaagatg
    26521 tagcggtgat aaacaaacct caaggaatgg tcgttcatcc ttcagcaggt catacgcatg
    26581 gcacaatggt caatgcgctg atgtaccata tcaaggatct ttctacgatc aacggcgtta
    26641 ttcgtccagg tattgttcac cgaatcgaca aagatacttc cggactgcta atggttgcca
    26701 aaaatgacca ggcacacgaa tcattagctc agcaattaaa agataagacc tctttgcgca
    26761 aatatgttgc tttagtccat ggagagattc ctcacgaaaa aggaaccatc aatgcgccaa
    26821 tcggccgttc gaaggaaaac cggaaaatgc aggcgataag agaagatggg aagtcggctg
    26881 tcacacattt tactgtattg gaacgtttcg aaggatttac tctggttgaa ttaaaactag
    26941 aaaccggaag aacacaccag atccgtgtgc atatgaagta tatcggttat ccgttagctg
    27001 gagatccagt atacggaccg aagaaaactt tgaaaggcaa tggtcagttt ctccatgcgc
    27061 aaatgttagg ttttacgcac ccgactacaa acgaaaagat gatctttgaa gcacctcttc
    27121 cagaagtttt tgaaaaaact ttagaaaaat taagaaaaaa cattgcattt taatttagat
    27181 aacggtatag taattaacaa cgaaagcata aaacttaata gaattccttt aagtttagtc
    27241 ccgtgaggct aagaagggtg ggagcaagga tacgtatggt gcgccataag tagattgctc
    27301 attgacatag tatagctatc agtgtatcag tatacgctca gtctaaacat tcctcctgtc
    27361 ctcttagggc aggagttttt ttatgcaaaa aagtaggggg aatgaaaatg caagcaaaag
    27421 aagtagtaga tcaggtaacc atgaagcgag cattgactcg aattacttat gagattatcg
    27481 agagaaacca tagtatccaa gatatcgtat tggtaggtat caaaacacga ggaatctaca
    27541 ttgcatcaag aattgccgaa cgcctgaagc agctagaaga tatcgatatt cctgtaggag
    27601 aactggatat cactttatac cgagatgaca aaaaagaaaa tccagaagaa ccggaacttc
    27661 attcttctga catcccagtt tccttagaag gaaaagaagt tattttgatt gatgatgttt
    27721 tgtatacagg acgaacgatt cgagcagcaa tggacgcagt aatggatttt ggcagaccaa
    27781 gaaaaatttc attagcagtc ctggtcgatc gtgggcatcg agaattgcca atccgtgcag
    27841 attatgttgg gaagaacatt ccaacatctc gggcggaaga gattttagta gagatgcaag
    27901 agcttgatgg acaagatcgc atcatgattt tgaaagagga agattagagg agagagaaac
    27961 atgtcggaga aaaaaggatt tcataatgat gacgtggtgt tagatatcca cgatcgccca
    28021 accccaggac attgggtagg attaagttta caacacttgt tcacgatgtt cggagcaacc
    28081 gtgttagtcc ccatcttagt aggtatcgat ccaggtattg cgctagtcag ttcaggacta
    28141 ggtacattgg tgtacttatt tactactaaa ggaaagatac ctgcgtactt aggaagcagc
    28201 ttcgctttca ttgcagcgat gcagcttttg atgaaaacag atggttatcc agcgattgca
    28261 caaggagcag tcacaactgg attagtctat ttgattgttg cgctgatcat caaaaagatc
    28321 ggttcagcat ggctggataa aatcttgcct ccaatcgtcg tcggtccagt catcatggtt
    28381 atcgggttgg gacttgcggc aaatgcagca acaaatgcta tgtataatca gggcacgtat
    28441 gatttcaagt atattgctgt agcgctgatc acattaggat taacaatttt cttcaacatg
    28501 tggctaagag gttttctcgg attgattccg attttactag gtattattag cggatacatc
    28561 gtagccttgc tgtttggaat cgtggatact aagccaatcg tagacgctgc ctggtttgct
    28621 gttcctaact ttgagattcc ctttgtacaa tatgaaccaa agctatatct tggagcaatc
    28681 acaacaatgg cacccatcgc ttttgtgacg atgacagagc atatcggcca tcttatggta
    28741 ttgaataaac taactaaacg taatttcttt gaagaaccgg gtcttcataa gacgttgaca
    28801 ggtgatggat tagcacaaat cgtggcagcc tttgtcggag gtcctcctgt aacaagttac
    28861 ggtgaaaaca ttggtgttct ggctatcaca cgtgtccata gcgtgtttgt gatcggtgga
    28921 gctgcagtat ttgcagtcat tttaggattc gtaggaaaat taagcgcttt gattttaagt
    28981 atccctggac cagtcatctc aggaatcagt tttatcttat ttggagtaat tgccgcaagt
    29041 ggaatgaaga ttttaatcga gaacaaaatc gattttgata aaaagaaaaa tctgttgatt
    29101 gcttcagtta tcttagtagt aggaattggc ggactcgtct ttgaagtagg cacatttaca
    29161 ttgtcggcaa tggcattagc aactgtactt ggcatcattt taaatcttat cttaccagaa
    29221 acttcacgaa gcgaagaaaa ctaaggagga aacattatga tcatcaaatc agaacgtatc
    29281 agcttaaagc atttattgac cgttgaggca cttactgatc aagaagttat gggcttgatc
    29341 cgcagagggc aggaattcaa gcaaggtgct aattggactc cgcaaaagcg ccagtacttt
    29401 gctacaaacc tctttttcga aaatagtact cgcacacata aaagcttcga tgtagcggag
    29461 aaaaaactag ggctagaagt gattgaattt gaagcaagca caagctctgt ccaaaaaggg
    29521 gaaacactat acgatacagt actgacgatg tcagcattag gcgtagacgt agcggttatc
    29581 cgtcatggtg atgagaacta ttatgacgaa ctgatacaaa gtaaaacaat ccagtgttcg
    29641 attatcaatg gaggagacgg aagcggacag catccaaccc aatgcttgct tgatttgatg
    29701 accatttatg aagaatttgg tcattttgaa ggtctgaaag ttgccatcgt aggtgatatc
    29761 actcactctc gcgtagctaa atccaatatg cagatgttaa aacgtctagg cgcacaagta
    29821 ttcttctcag gtcctagaga atggtacgac gatgaattcg aagtgtacgg acattatatg
    29881 ccattggatg aactactaga tcaagttgat gtcatgatga tgcttcgggt gcaacacgaa
    29941 cgccatgatg gaaaagaaag tttctcaaaa gaaggctacc atcaggaaca tgggttaaca
    30001 gttgagcgtg cgaagaagtt gcaaaaacat gcaatcatta tgcacccagc accagttaac
    30061 cgtgatgtag aattagctga ttcattagtg gaaggattac agtcgcggat cgttcagcaa
    30121 atgagcaatg gagtattcgt cagaatggca atcttagaag cagtgctaaa tggaaaagcg
    30181 taattatagg aggatgaaaa aatgaaaaca ctcattaaaa acggtcagat caatacacga
    30241 aaaaatatga cgacaccagc agaaatttgg atagaagatg ggcgaatcaa agcaatcggt
    30301 acaggattct cagaagccga atttgatgaa gtgtttgatg caaagggaca attgatcaca
    30361 cctggtttag tggacgtcca tgttcatttg agagagccag gatttacata caaggagacg
    30421 atcgaagctg ggactcgctc agctgctcgt ggcggtttca ctactgtatg cgcgatgccg
    30481 aatttaaatc ctgtacctga tacggcagaa aagctgagac aagtatatga catcattaga
    30541 aaagatgcag ttgtgaaggt attgcagtat gcgccaatca cagaaaatct gcgaagtgag
    30601 aaattagttg atcaagaagc actgattgaa gaaggtgctt ttgctttcac aaatgacggt
    30661 gtaggtgtcc aaacagctgg gacgatgtat ctggctatga aagaagctgc aaaaaacaat
    30721 aaagccctag tagcccatac agaagatgaa tctttattat ttggcggcgt tatgcacgct
    30781 ggcaagaagg cagaagaact tggtcttccc gggatcttaa gcgtaaccga gtcttctcaa
    30841 attgctagag atctgttgct agctgaagca actggtgttc attaccatgt ttgccatgtt
    30901 tctactaaag aaagtgtccg tgtaatcaga gacgcaaaaa aagcaggaat ccacgtaaca
    30961 gcagaagttt caccccatca tcttattttg atcgacgaag atattcccga agatttcggc
    31021 ttttggaaaa tgaacccgcc gctaagagga cgggaggaca gagaagcttt gatcgaaggc
    31081 ttgcttgacg gtacgatcga ttgcatcgct acagaccatg ccccccatgg attagaagaa
    31141 aaaagccaaa gtttcatgaa atcaccattt ggtatcgtag gaagcgaaac agcgtttcaa
    31201 ctgatctata ctcattttgt agaaactggt cgattcacgt tggaacaagt gatcaattgg
    31261 ttggcagtta aaccggctga gatttttggt ttgaatgcag gaacgctaac ggttggtgcc
    31321 cccgctgatg tagcagtttt tgatattaca caaacttgca cgatcgataa agaagacttt
    31381 ttatcaaaag gggagaatac accttttatc ggctggaaag taaaaggcga aacacaaatg
    31441 acttttgtca acggaaaact tgtgtggcag aagggagaat aaaagtatgg aacgtttgct
    31501 tatattagaa gatggaacag ttttcgaagg aaaggctttt ggtgcagatg ctagtgtctt
    31561 cggagaaatc gtttttacaa caagtatgac tggttatcaa gaaactatca ctgatcaaag
    31621 ttttaacggt caaatcatta catttaccta tcctatggtg ggtaattatg gggtaaatag
    31681 agatgattat gaatcgattt ctccaacatg caaaggagta gtagtaaaag aacatgcacg
    31741 tgtagcttca aactggcgaa accaaatgac gctagattca tttttgaaac aaaaaggaat
    31801 tcctggaata tcaggtatcg atacgcgcgc ccttactcgc aaaatccgtg aagtcggaac
    31861 gatgaaagct agtatagttg atgctggaga ttccttcgaa catgcatttg atcagctgaa
    31921 agcaaccgtt ctagcaacca atcaagtgca gcaagtctca actaacaaac cttatccaag
    31981 tccaggaaca ggtcggaaag tcattgtcat tgacttcggc ttgaaacata gtattttacg
    32041 agaattgagt aaaagaaact gtaacctcac agtacttccc tataacacag atgccgagac
    32101 gattttagcg ctatcaccag acggagtcat gctgacgaat ggacctggtg atccaaaaga
    32161 tgtgccagaa gcaatcgaaa tgatcaaaaa aatccaagga aaagtaccga tttttggtat
    32221 ttgtctcggt caccagttat ttgctttagc caacggtgca gatacctata aaatgaaatt
    32281 cggacatcga ggattaaatc acccggtacg agaagtagcg accggaagaa ttgattttac
    32341 ttctcaaaat catggctatg cagtcgatga agccacgatt gatccagaaa aactcatcgt
    32401 aactcatgta gaagtaaatg acggaacagt agaaggagtc aaacacagag attatcccgc
    32461 tttcagtgtg cagtttcatc cagatgctgc ccctggtccg catgatgcac tacatttatt
    32521 tgatgaattt atggaaatga tggacgcagg aaaggagcag taaacagatg ccgaaacgta
    32581 cagatatcaa gaaaattatg gtgattggat ctggtccgat catcattggt caagccgcag
    32641 aatttgacta cgcaggaaca caagcctgcc ttgccttaaa agaagaagga tatgaagtgg
    32701 ttttagtaaa ttctaatcct gcgacgatca tgacggataa agaagtggca gatcaagtat
    32761 atatcgaacc gatcacactt gaatttgtat caaggattct tcgaaaagag cgtccagatg
    32821 ctctccttcc aactttaggc ggacagacag gcctcaatat ggcaatggag ctggcagcat
    32881 caggtatttt agaagaattg aacatagaac ttctaggaac aaagctttct gcgatcgatc
    32941 aagcagaaga tcgtgatcta ttcaaacagt tgatgaaaga attgaatcag ccaatccccg
    33001 agtctgaaat cgtcaataca gtagaagaag ctgtagattt tgcaaataaa atcggctatc
    33061 ctgtcatcgt tcgtccagcc tttactttag gcggaactgg tggaggtatg tgtgaaaatg
    33121 aagaagaatt acgtatcgta tcagaaaatg gattaagtct ttcccccgct acccaatgtt
    33181 tgattgaacg aagtattgca ggttttaaag aaattgaata tgaagtgatg agagattcgg
    33241 ctgataatgc aatcgttgtc tgcaatatgg aaaattttga cccggtgggt atccatacag
    33301 gtgattctat cgtatttgcc cctagtcaaa cattgtctga ccatgaatat caaatgcttc
    33361 gtgatgcttc tctttcgatt attcgagcac ttaagatcga gggaggctgt aatgtccaac
    33421 tcgctcttga tccacatagt tttaattatt atgtgattga agtcaatccg cgtgtttctc
    33481 gttcatctgc gttagcaagt aaggcgactg gctatccgat cgctaagcta gctgctaaaa
    33541 tagcgcttgg attaacatta gacgaaatga aaaatcctgt tacaggaacg acttttgcag
    33601 aatttgaacc tgcattagat tatgttgtag ctaaaattcc tcgctggccg tttgataagt
    33661 ttgaaaacgg agaacggctt ttaggcacgc agatgaaagc aactggtgaa gtgatggcta
    33721 tcggtcggaa tatcgaagag tctttattga aagctgtgcg ttctttagaa atcggtacac
    33781 accatatcga acttcctgag ttggcagaca tcacggacga tgacctaatc caaaaagtgg
    33841 ttcgagcaca agatgatcgc ctgttttatg tagctgaagc tattagaaga gggtacacca
    33901 tagaagaatt agcggatttg acaaaaatcg atttattctt tttagataaa ctgttgcata
    33961 tcgtagaaat cgaacagcag ttgaaacagc agccgttgga tgacaagata ttgaaaacag
    34021 cgaaacaaaa cggttttaca gacaaaaaag tggcggagct gtgggaaata aacgaagaag
    34081 ccgttcgcca gatgagaaaa gaaaatgatt tgattcctgt atataagatg gttgacactt
    34141 gtgcagcgga attcgaatca aaaacacctt atttttatag ttcctatgaa tatgaaaatg
    34201 aaagttcacg atcagaaaag ccctcaattc tagtactggg atctggtcct atccgtatcg
    34261 gacaaggggt tgaatttgac tatgcgactg tgcattctgt caaagcaatc cagcaggcag
    34321 gatacgaagc aatcatcatg aacagcaatc ctgaaacagt ttcgactgac ttttctattt
    34381 cggataaatt atattttgag ccattgacct tcgaagatgt gatgaatgtg atcgagctag
    34441 aacagccgat aggagtaatc gttcaatttg gtggtcagac agcaatcaac ttagccgaac
    34501 ctttgactgc tgctggtgta aaaatcttag ggacaacgat cgaagacttg gatcgtgcgg
    34561 aaaatcgtga tttattcgag caagcgctga aagagttgga tattcctcaa cctttagggg
    34621 atactgctac aagtaaagaa gaagccgtgg ttatcgcttc gagaattggt tatccagttc
    34681 ttgttcgtcc aagctatgta ttaggcggac gtgcgatgga aatcgttgaa aaccagcgag
    34741 atttagaaga ttacatggaa catgctgtga aagcatctcc agaacatcca gtactagtgg
    34801 accgttactt gcttggtggt gaatgcgaag tggatgcgat ttgtgacggt gaaacagtac
    34861 tcatcccagg gatcatggag catattgagc gtgctggtgt ccattcaggg gattcaatgg
    34921 ctgtgtatcc acctcaaaca ttatctgaat cgatcaagaa aacgatcgaa gattacacga
    34981 tccgcttagc attaggtttg aattgtgtcg ggatgatgaa tatccagttt gtcatccatg
    35041 aaaatgaggt ttatgtgatc gaagtcaatc ctcgagcaag tcggactgtt ccattcttaa
    35101 gtaaggtgac aggaattcca atggcacaaa ttgcgacgaa agctattttg ggagagaaac
    35161 taaagacact tggttatgaa aacggacttt atccagaaac aaaagaagtt catgttaaag
    35221 cacctgtctt ttcttttacg aaattgcaga aagttgatac ttacttaggg cctgaaatga
    35281 aatcgactgg agaagtaatg ggatctgaca gacaattaga caaagctttg tacaaggcat
    35341 tcgaagcttc tggattacac ttgcctgaat atggagccgt attatttacg attgcggacg
    35401 aaacaaaaga agaagcacta gaattagcta aacgttttac agaaattggc tatagtctga
    35461 taggaacgaa acatacagct gcttatttcg aaaaaaatgg tctggtcgtt gaaacagtag
    35521 cgaaaatttc tgaggaagca gcagaaaaaa atgtagtcga cttaataaga gatggtaaaa
    35581 cacaagtggt tgtcaatacg attgataaag atcggggaaa tgcttctaaa gacggtttca
    35641 tcatccgaag agaagcagtt gaacatggta ttccattgtt tacttcttta gacacagcag
    35701 atgcgatcgt tcgagtgatg gaatcccgtg ctttttcaac taaccctatt taacggcgag
    35761 cacatgttaa gacaaaaaga cgaaaaaccg ttgcaggagg ggacagatga aacaagaaat
    35821 gatggaaatc gttcagcaaa aacagctggc accaaagatt tttgaaatga cattgaaagg
    35881 tgatctagta aaagaaatgc agcaaccagg acagttcttg catattctcg tgccgcgtgc
    35941 agacttgcta ttacgccgac cgattagctt gaatcatatc gaccacgaaa caaatacttg
    36001 ccgtatcatt tatcgggtgg aaggtgaggg gactagagtc ttctctactc tttctgccgg
    36061 agaccattta gatgtcatgg gtcctttagg caatggtttt gatttgtcca atcttcatac
    36121 aggagacgaa gtatttataa tcggtggagg aatcggtgtc cctcctctat atgagttatc
    36181 aaaacagtta gtcgaaaaag gaattaagcc aacgcatttt ttaggttttg ccacacatga
    36241 agtcgtttat tatgaaaaag aatttaatcg tctaggagaa acaagaatcg ctactgacga
    36301 tggaacatac ggtatgcacg gaaatgtcgg taatttattg ttaggaacga agacggaacc
    36361 tacagctgtt tttgcatgtg gttcaaacgg gttattgaag acagtggaac agctgttttc
    36421 ttcgcatcaa aatgtccagt tatcattaga atcccgcatg gcatgtggga taggggcttg
    36481 ctatgcttgc gtttgtcata cgccggaaga tgaaacagga acaaaaagtg tgaaagtttg
    36541 tgacgaagga ccgattttta aaataggaga ggtaattata tgaatgagaa tcctttagcg
    36601 gtcaaacttc ctggcctaga attaaaaaat cctatcatgc cggcaagcgg ctgtttcggt
    36661 tttggcaaag aatacggaaa atactatgat ttgaatcagc ttggatcgat catggtcaag
    36721 gcaacgactc caaatgcccg ctttggcaat cccacgccaa gagtggcgga aacaccatct
    36781 ggcatgttga atgcaattgg tttgcaaaat cctggtctag acgttgttat gcataaactt
    36841 cttccagaat tagaggagta caatgagtta cccatcatcg caaatgttgc tggtgcttgt
    36901 gaagaagact atgtagaagt ttgtagcaaa attggcgaag caccaaatgt gaaagcaatc
    36961 gagctgaaca tttcttgccc aaatgtcaaa catggcggaa ttgcttttgg aacagattct
    37021 gatgttgctt ttcaattaac acaagcagta aagaaagttt cttctgttcc tgtctatgtc
    37081 aaattgtccc caaatgtaac agacatcgtt ccaatcgctc aggcaatcga agctggaggg
    37141 gcagatgggt ttacgatgat caatactctt cttggtatgc ggattgacct taagacaaga
    37201 aaaccgattt tagccaatca aacaggtgga ctctccggac cagcaatcaa gcctgtcgct
    37261 attcgtttga ttcaccaagt agcaagtata tccagccttc caatcattgg gatgggcggc
    37321 gtgcagacag tggatgatgt tcttgagatg tttatggctg gagcaagtgc tgtagctata
    37381 gggaccgcga atttcaccga tccgtatatc tgtcctaaac taattaaaga gctccctgtg
    37441 cgaatgagcg aactaggcat cgaatcgctg gaaagattaa tcaaagaagt gagagaggaa
    37501 cgtgaacgat gaatcaacta gaccaacgac caattattgc attagatttt tcaactaggc
    37561 aagaggtaga agactttctt cgatttttcc cgaaagaaga aaaattgttt gtgaaaatcg
    37621 gaatggaatt gttctaccaa gaaggacctg aaatcgtacg ttatctaaaa gaggctggtc
    37681 ataatgtatt tttagatctg aaacttcacg atattccgaa tacggtcgaa aaagcaatgc
    37741 ggggattggc aaagcttggt gttgatctca cctgtgttca tgccgctggc ggtattcgta
    37801 tgatggaggc ggcaatgcga ggattagaag aggggactcc agaaggtgga aaacgtccgc
    37861 tacttctagc tatcactcag cttacttcta caagtgaaga agaaatgcat gccgatcaac
    37921 taatcgaagt ttctttagaa aaaagtgtga tccattatgc tagctgtgcc aaaaaagcag
    37981 gattagatgg tgtggtttct tctgcttggg aagtagaagc gatcaaagaa atggcaggag
    38041 acgaatttgt ttgcttaact ccgggtataa gaccagaagg aacagtcgca ggagatcaga
    38101 caagagtagt cacaccttct caggcaaaaa agatcggctc aacttttatc gttataggtc
    38161 gaccaatcac tcagtcaact aatccttata aagcatatca aacgattcaa acagaatgga
    38221 gctaacctaa aatgaatgtt gaacaaagta tcgcaaaaga cttattagaa attgaagcag
    38281 tatttttaaa tccaagtaat ccttttacat gggcaagtgg aatcaaaagc ccaatttact
    38341 gtgataaccg aatcacgatg agctatccaa aagtccgaaa agagatcgca aaaggattag
    38401 caagcaaaat caaagaagct taccctgaag tacaagtgat tgctggaact gcaacagcag
    38461 gaatccctca tgccgcatgg gtagcggaaa tactggattt gccaatggtc tatattcgaa
    38521 gtaaggcgaa agatcatggg aaaggtaacc aaatcgaagg acgtatattt gaaggtcaga
    38581 agatggttgt gatcgaggat ttgatttcta caggcggaag tgtcttggaa gcagctgaag
    38641 cagcaaaaag agaaggtgcg gatatccttg gtgttgcggc tatctttacg tatgagttgc
    38701 caaaaggaaa agcaaatttc gaaaaagccg aaatccctct tatgacgttg accaattatt
    38761 ctgtattgat tgaagcagct ttagaagatc gttatatcga tgagcaagaa ttaactctgc
    38821 taaaagaatg gaaaaaagat cctgaaaatt ggcagattta agcgcgcttg atgaagatag
    38881 agaaatccga tttgtgtctc cagaatcatc aattggaata gcaaaaatcc aaaaactgat
    38941 tgcgagaagc atcttttcat caggtatgaa aagaaacttc tatcgtgatc agtttttgat
    39001 ttttttaaaa ttcatttttc taagactggt tagcagaaag aagagaataa tctcttctct
    39061 tttttctatt atatatagat tttctctatt ttttggtaag ggagctgttt caaaaatggt
    39121 aaagagcgga aatgttgcga atatcagtta tttaatgata tgatattcgg aaaaagaaat
    39181 ctcaaaggga ggtaactttt atggggttga atgaatttat tttaggattg aacaatttag
    39241 agacgatcac aagggcacct ggatttttta aatatacaga gcatacagtt gcagcacatt
    39301 cgtatcgtgt agcatctatt gcgcaagttc taggagacat tgaagaagcg aatggacaac
    39361 cgatcgattg gaaactgctc tatgaaaaat cattgaatca tgactacaca gagcgtttta
    39421 ttggtgatat caaaacgccg gtgaaatacg caagcaaaga gttaagaggg atgctgcaaa
    39481 cagttgaaga aaagatgacg gacgaattca tcaaccaaga gattccagct gagttccaag
    39541 aagtttatcg ccgtcggttg ttcgaaggaa aagatgaaac cgtagaagga gaactattag
    39601 ccatttcaga taaggttgac cttctttatg aatcttttga tgaaatcatc aaaaataacc
    39661 cggaaaaagt ttataaggag atgttcattg aggcagtgac tacgattaaa agctttgaac
    39721 atcggccttc tgttcagtat ttctttaaag agatatttcc ggaactattg aaccaggaat
    39781 tttatggaaa agaggcgttt ttagaaaccg tctcaacgat tgtaaagaaa gaagaaaaat
    39841 aaaaatgata tgagatgcag ggggaagtat agttgaaaaa gaatatggat gtcgaatggg
    39901 gctattctgg agacaaaggt ccggaaaatt gggcgagttt atgtgattgg tttgcgagag
    39961 gtgcggaatt tccattgcag tcgcctattc atttgaccaa gggggaagaa accaaaatag
    40021 aaggagacac gctttccttt cattatcaaa agcaacggtt tactgacaag gaattcaaaa
    40081 acacgattca tttagtacct tttgaccaac tcagttatgt agagttcaag aaagaacgct
    40141 attatttgac agatattcat ttccacttgc cttctgaaca tatcatcaac ggaaaacagg
    40201 aagatattga atttcatttt gttcatatga acagcaaaaa agagaatcta gttgtgggtg
    40261 tcatgtacca gctaatggta caggaaggtt ggctgtataa tcaagaaaat ggggaaagtt
    40321 ggaacttaga aaaacatgag cattgggtaa atccggatgt gttctttccg gagcaaaaaa
    40381 gccatttcca ttatattggc tcattgacaa ctccgcctac cagcgggcca atccaatggt
    40441 ttgtaatgga tcatgtagga aaactgaacc aagagtttct tctccccttc gatggtcagt
    40501 atgctcgacc gaacaaccga ccaatccagc ccttgaatgg aagagaaatt tattatttcg
    40561 aggagtttga gcaatgaata ttcagcagat gaaatacgtg gtcgctgtag caaacaatgg
    40621 tagtttcaga gaagcggcaa agaaaatgtt tgtctcccag ccaagtcttt cagctggtat
    40681 caaagaatta gaaaccgagt tagggatcac gctgtttact cgaacgaatc gaggggcata
    40741 tttaacagaa gaagggcaag acttcttaaa tcgtgccgag aaaatattgg tccagcttga
    40801 tacattagaa actcactatc taacgagtga aaagagagac aatttttcga tcgctgccca
    40861 gcattatgat ttcttaggtc ctttgaccgc acagctactc gaggaattca aagacgagat
    40921 caaacaattt cggatcatgg agacgacaac agcgagagtg atcgaagaag tcaaagagca
    40981 gcacagtgag atcggcatta tttatatgaa tgagcataat caagctggta tcagaagata
    41041 tttacaacaa ggagagctaa gtggccgcag cattggaaaa ttcaaaacgc atattttttt
    41101 aaggcaagga catccattag ctgaacacgc agagatcacc aaagaagaat tgttatctta
    41161 tcctcaagtt cgttttacac aggatggaaa taatttcccg tatttttatg aagaccttat
    41221 cgagatccct gatcaagaaa cagtgattta tacgagtgat cgaggtactt tgatgaatat
    41281 cgttcttgaa actgatgctt acgcttcagg atctggtatt gtgattggag ggattcgcga
    41341 gcacttgcgg ttaatccctt tggcagaagg cgaactaaat gaattttata tcatttattc
    41401 ggcgaaacgt ccgctcagtg aaatcgctca aagatttatc aaagagctta ctcggttact
    41461 ggaccagcag agctaagaaa agcagatgag aacgctgttt attgaacacc gttgatcaaa
    41521 aaagaggggt tgtgacagaa ttttgtcaca actcctcttt ttgtagcaaa c
//
LOCUS       DACDIG010000019        41202 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.019, whole genome shotgun sequence.
ACCESSION   DACDIG010000019 DACDIG010000000
VERSION     DACDIG010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.019"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(16..708)
                     /locus_tag="IV247_001055"
     CDS             complement(16..708)
                     /locus_tag="IV247_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8777140.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            703..969
                     /locus_tag="IV247_001056"
     CDS             703..969
                     /locus_tag="IV247_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8777141.1"
                     /translation="MHRTFGSRGCPTLHLADRYAERKTPTLCFAFSARTRQAGIPLED
                     IKDFLGHKDVSTTQVYAHISPEVKKRSMNQLENYIEEQIKKHSN"
     gene            complement(1145..1714)
                     /locus_tag="IV247_001057"
     CDS             complement(1145..1714)
                     /locus_tag="IV247_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP8777142.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1788..2111)
                     /locus_tag="IV247_001058"
     CDS             complement(1788..2111)
                     /locus_tag="IV247_001058"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777143.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVITDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2265..3077)
                     /locus_tag="IV247_001059"
     CDS             complement(2265..3077)
                     /locus_tag="IV247_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777144.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3431..4279
                     /locus_tag="IV247_001060"
     CDS             3431..4279
                     /locus_tag="IV247_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAP8777145.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPRPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4424..5365
                     /locus_tag="IV247_001061"
     CDS             4424..5365
                     /locus_tag="IV247_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAP8777146.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLKKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFTHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5355..7526
                     /locus_tag="IV247_001062"
                     /pseudo
     CDS             5355..7526
                     /locus_tag="IV247_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7638..9809)
                     /gene="copB"
                     /locus_tag="IV247_001063"
     CDS             complement(7638..9809)
                     /gene="copB"
                     /locus_tag="IV247_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAP8777147.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(9829..12015)
                     /locus_tag="IV247_001064"
     CDS             complement(9829..12015)
                     /locus_tag="IV247_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAP8777148.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(12015..12224)
                     /gene="copZ"
                     /locus_tag="IV247_001065"
     CDS             complement(12015..12224)
                     /gene="copZ"
                     /locus_tag="IV247_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAP8777149.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12237..12677)
                     /locus_tag="IV247_001066"
     CDS             complement(12237..12677)
                     /locus_tag="IV247_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAP8777150.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12751..13290)
                     /locus_tag="IV247_001067"
     CDS             complement(12751..13290)
                     /locus_tag="IV247_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8777151.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
     gene            complement(13905..15371)
                     /locus_tag="IV247_001068"
     CDS             complement(13905..15371)
                     /locus_tag="IV247_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8777152.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(15682..17763)
                     /gene="cadA"
                     /locus_tag="IV247_001069"
     CDS             complement(15682..17763)
                     /gene="cadA"
                     /locus_tag="IV247_001069"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP8777153.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            complement(17905..18084)
                     /locus_tag="IV247_001070"
                     /pseudo
     CDS             complement(17905..18084)
                     /locus_tag="IV247_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            complement(18288..18647)
                     /locus_tag="IV247_001071"
     CDS             complement(18288..18647)
                     /locus_tag="IV247_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAP8777154.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(18677..19018)
                     /locus_tag="IV247_001072"
     CDS             complement(18677..19018)
                     /locus_tag="IV247_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777155.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(19015..19689)
                     /locus_tag="IV247_001073"
     CDS             complement(19015..19689)
                     /locus_tag="IV247_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP8777156.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKSLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(20741..22039)
                     /gene="asnS"
                     /locus_tag="IV247_001074"
     CDS             complement(20741..22039)
                     /gene="asnS"
                     /locus_tag="IV247_001074"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAP8777157.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(22079..23269)
                     /locus_tag="IV247_001075"
     CDS             complement(22079..23269)
                     /locus_tag="IV247_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP8777158.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(23290..23817)
                     /locus_tag="IV247_001076"
     CDS             complement(23290..23817)
                     /locus_tag="IV247_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP8777159.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGYESGDEVKTIKGI"
     gene            complement(23864..26653)
                     /locus_tag="IV247_001077"
     CDS             complement(23864..26653)
                     /locus_tag="IV247_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP8777160.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKATLKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(26800..27000)
                     /locus_tag="IV247_001078"
     CDS             complement(26800..27000)
                     /locus_tag="IV247_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8777161.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(27452..27772)
                     /locus_tag="IV247_001079"
     CDS             complement(27452..27772)
                     /locus_tag="IV247_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777162.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHILHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(28275..29393)
                     /locus_tag="IV247_001080"
     CDS             complement(28275..29393)
                     /locus_tag="IV247_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777163.1"
                     /translation="MAKKDKELLSQLISEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FSKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            29866..31173
                     /locus_tag="IV247_001081"
     CDS             29866..31173
                     /locus_tag="IV247_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAP8777164.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKVSGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANMFVIGWRRVAEFFGNLF"
     gene            31291..32490
                     /locus_tag="IV247_001082"
     CDS             31291..32490
                     /locus_tag="IV247_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777165.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRVIFSSNNYHIF
                     RAGLYACQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(32517..33785)
                     /locus_tag="IV247_001083"
     CDS             complement(32517..33785)
                     /locus_tag="IV247_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAP8777166.1"
                     /translation="MEKQLPSPMRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DTRLSHPWLLYLLPVSGALFAYLYVYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(33948..34595)
                     /locus_tag="IV247_001084"
     CDS             complement(33948..34595)
                     /locus_tag="IV247_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HAP8777167.1"
                     /translation="MEQTIKPYQYFFIKREVEQLLNAYSSVNDPKTVQTVQALAAEKI
                     RDILDHELLEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMVYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     gene            complement(35003..35506)
                     /locus_tag="IV247_001085"
     CDS             complement(35003..35506)
                     /locus_tag="IV247_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8777168.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(35629..36393)
                     /locus_tag="IV247_001086"
     CDS             complement(35629..36393)
                     /locus_tag="IV247_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAP8777169.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLADVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            36500..36775
                     /locus_tag="IV247_001087"
     CDS             36500..36775
                     /locus_tag="IV247_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAP8777170.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            37371..38321
                     /gene="yidC"
                     /locus_tag="IV247_001088"
     CDS             37371..38321
                     /gene="yidC"
                     /locus_tag="IV247_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP8777171.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPADNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(38500..39474)
                     /gene="lacD"
                     /locus_tag="IV247_001089"
     CDS             complement(38500..39474)
                     /gene="lacD"
                     /locus_tag="IV247_001089"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP8777172.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDTKMPGR
                     LPDLLNIWSVKRLKEAGADACKFLLYYDVDEPAEINVQKQIVIERIGSECEAEGIPFF
                     LEILSYDTTIGDPSSLAYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QETCYSQAEASAYFYEQSKVTEQPFIFLSAGVNATLFQDTLRFAKQSGSTFNGILCGR
                     ATWAKGVAPFVTEGEKKASSWMLEEGKQNIKELSTLLKETATPVYVDR"
     gene            complement(39471..39644)
                     /locus_tag="IV247_001090"
                     /pseudo
     CDS             complement(39471..39644)
                     /locus_tag="IV247_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
     gene            39806..>41202
                     /locus_tag="IV247_001091"
     CDS             join(39806..40379,40379..>41202)
                     /locus_tag="IV247_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8777173.1"
                     /translation="MAKYTFELKLKIVHDYLDGKGGSDYLAKKYSIKAPSQVKRWINA
                     YQEFGEEGLVRKRQKKKYSVQFKLDAIELYLTSELSYREVANTLNMNNPNLIASWMRQ
                     FREGGIDGLSKTKGCPPILSKKNEPKNKKKSTTKATSKERERIEELEKRVRSLQIENA
                     FLKELRKLRKQEAQQRRMKQSHESSQASEDRFKLVELLETLKFPKATFMYWQKRLDRK
                     NPDQEIEAEMLKIREKHKDYGCLRMTNELRNRGFSINKKKVQRLIKKLGIKVTAYTRK
                     SRRYNSYRGQVGAVAKNRIHRRFYTSICHQKITTDTTEFKYHEADAKGIIRQKKLYLD
                     PFMDMFNSEILSYRISEKPNALAVMEGLEEAVQTTNDCPYRRMFHLDQGWAYQMKAYR
                     NKLKEYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYY
                     NNERIKQKLNWQSLVQ"
BASE COUNT        11887 a         8628 c         7329 g        13358 t
ORIGIN      
        1 agaggggggg attttttatc gttgtggaat atttaaatac caacgcttgt catgaaaatc
       61 ttgtgctcca cccttagtat ttccttctgg atcgtttgtc gcacgcatca tgacgtatac
      121 tttcttatta gggaaatcac gcatattgaa agatacatga tagccaacat ttcctaaagt
      181 attataagct tgatttacgt ctggtctata aatcccatca gctcttactc tagctagttc
      241 ttttccagta ttgtagtcca taatgaaaat atactcgtat ttataattag caatgtgcca
      301 tccagctaca tgcaagtttg cgttttcgat ttctccgaac tgatcaatgt gggcgtaatt
      361 tgttccatct gtcagcgtag aatttgcagc acctgctcga gttggatcaa ttactggttt
      421 atcctccgaa gtagttggat tatcatccgt aaaaccatga gccaaatcat aggctagttt
      481 ttctttactt acacccattt cagaaagata accgtaagga tctgtatgat cgccccaaat
      541 attttgtgtt acccataaat gcgatttgat tcctggttgg ttataaggag tgtctaatgt
      601 taatggaata ccatatttca ttgctgaatc tcttgccaat tcaacgtata ctttatagtt
      661 cttttcaaaa gtttctttat catgtgtgtg ttgtaactcg atttgcacag gacttttggc
      721 tcacgaggtt gtcctactct acatcttgct gatagatatg ctgaaaggaa aacaccaaca
      781 ttatgttttg ctttttcagc tagaacgaga caagcaggaa tacctttaga agatatcaaa
      841 gattttttag gtcataaaga tgtttccact acacaagtct atgcccatat ttctcccgaa
      901 gtgaaaaaaa gatcaatgaa tcaactagaa aactatatag aagagcaaat aaaaaagcac
      961 tcaaattgag tgctttttta agctcactat cactaaaact atcactaatt ttatttataa
     1021 gcgaacatgt ttctatcact gattttttaa atctagcgaa aaacccttat cttaaaggcg
     1081 ttcgttcaat tctttggcaa ggtcttcaaa gcctggcttg cctagaagtg cgaacatggt
     1141 tttcttatag ctcatgtaga aaaattttct tgatgatgat tttatccatc aattgagatt
     1201 gactgttttg ctctcctatg atttcagcta tgattgtccg attttgcggt tttcttctta
     1261 actggtagtt gattttccca gtttccatca actcgctaag catatccacg actccgccaa
     1321 atccggtctt tttcgtaata aaacctagtg ctaccattcg ttcatctaga ttttttgctg
     1381 taatcgtcac accagtaatg agcgagtgct gtaatgtttg ggccagctct tccatccctt
     1441 cagcattgga tacgacggaa gctgttttgt taataggttc ttcgtttatt tcttttggtt
     1501 ctttcctctc cactttcact gtttgacgaa ggacatctag ttcttgcaag agcgtttcgt
     1561 attgttcttg ggtcagcatc acgccagcta ctttttcacg attaaatata tatacgccag
     1621 ttcccgcgtc tctcgcttga gcaaatacat ccatcggcga tcgtttcact tctgtgatcg
     1681 aagatgtggg aacttctaat ttctttaatt ccaatggatt tcctccttta ctaatcgctt
     1741 tttattatag cacagactga cttgttttga tcttttttcc aaaggtttta tcggtttttg
     1801 cctaaccagt aattgatatc ggtaagaagt ctttcgatac caaatgaagg atggtacaga
     1861 tacgtttttt gatcttcgtt atggtctagt ggaaaaaaat gatcggtgat cactagatct
     1921 gcataccgag gcggcttatt tcgagtacat acatgtatag agatccgttt tcgatgtctt
     1981 agcgttttgt tcagtacccc agcaagtctt cttgcttctt ggtgttcata gcgaaaaaaa
     2041 aagacgattt tcacttcagg agggtggaca tctaaatacc ctcgttcctg gagtgttttc
     2101 agtaaaagca tcccccactc tactgcgggg tgtgcaagtg cttgttggat cgctagccga
     2161 aattgatata gtttatcttt ggaattggga aatttttcta atgtttcctg ataataacga
     2221 acttcttcta tcagccggtc atggatacca attacgtaac gaagtcattc ttggtaaagt
     2281 acttttgtca gctttgattt gctttcgctc tcaaattcgc tattcagcca aaacagcgaa
     2341 tcaagctttt cagtcacaat cgtcaatttc cgatagtctt gtgtctcttc tatccgtttc
     2401 caccgctttt cagtaatctg gttacagctc aagcgcaaaa ttgctagata atttttttct
     2461 tttattggca aaatctgctc ctctctaaat agcaattcag gaaacaattc aaagaatagt
     2521 gttcgactgt ctcctaaaat ctcgatttct gccggatcct ttctttgctt caataattgg
     2581 atgaacatct cagaaaagga ctgctctcca tcatctgcaa aaaaatgcgg aaaatctttc
     2641 aaaaagcaaa acagcagata tcttactttt ctttctgaac caaagatctg tgctttttta
     2701 taagaaaaag aaaggtggta ctcgccaaac cactttctca atcgctcttg cgtagaacga
     2761 tcgtacttct gctcgtaagg ttcattcaat agcagatttt tcagtaaaca aaacgggggt
     2821 gattggatca gaaaaacaaa caagagataa ggttcgctat atccttgatc ataaactaac
     2881 tgaacggttt tcttatgaag aatcagctga atgggacagt cgctttccgc caaacgtttc
     2941 agatgccgat ccactgtttt aacatcgatt ttcagttctt cagctaaatc agcttttcgc
     3001 cagatcaaat ctttcattag attgattagt gaaaataaat tctgtgcatt ttttgataat
     3061 agtttttgcc atctcatcga tcatttctca cctgatttct aaacatattg tttgatcaaa
     3121 atgggttcac tcctaaaaag ataaaaaact ccctttctat ttatttaaat acgcaaatct
     3181 tcctcattat tttttatttt actcaattta atcgtattct catttcaaaa cgaatgtact
     3241 ttaaaaaaac ataataagat cactttgctt gtaggatgta ttcccttcca ttacaatgaa
     3301 ataaatagtg attttatgag aaggtgatgt gaaaatggaa gaaaaaatag aagaatccaa
     3361 acaaaaaatc gatcagttga tataagaatt agaaaaagcg gtcatcggca aacgttcaac
     3421 tatccaattg atgatcgtgg ccctattagc aggtggtcat gtcttattcg aagatattcc
     3481 cggtgtaggt aaaacgttac tggtcaaagc actagcaaaa gctgtacagg gaaccttcag
     3541 cagagtccaa tgtactcctg atctgctgcc tagtgatatt cttggttttt ccgtatacaa
     3601 ttcacaatca aaagagtttg aatttcgtcc gagaccgatc tttaccacga tcttgttggc
     3661 agatgagatc aatcgtacga ctcctcgtac tcagtcagca cttttagaag caatggccga
     3721 gaatcatgca acgatcgaca acaatacata ccctctagac aatcattttt ttgtcttagc
     3781 tacgcaaaat ccaatcgagt acgaaggaac atatcctttg ccggaagcac agctcgatcg
     3841 ttttttattc cgactacaaa tcggttatcc ttcttttgat gacgaacttt tgctactttt
     3901 ggataaattc gaaaaagtgt tggataactt aaatgaagta ttgaacagtt atgaaataga
     3961 agaaataaaa acaaatgtgg cgaatgtctt tgtcagtccc gaagtggcat cttatgcctt
     4021 gcagttagtg tcagcaacaa gaagtcatgc tgccatccag cttggcatca gtccacgagg
     4081 aagcctttcc tttattcagg cagcaaaagc ttatgcttta atccatggga gaaattacgt
     4141 cacgcccaaa gatttgcagg atttagttcc ttacgtgttt tcccatcgcc tgatttttta
     4201 cgatcgttca ttgaaggaag acgaaaagaa acaaatcatt catactcttg ttgcacaaat
     4261 tcctattcct gtgaggtaga ttgaggtaga caatggaaaa aatcaaagat tatttcatta
     4321 attttgtgtt atttctggta tatttcatca ttgtgctttt tgctcttgta ttcaacaatt
     4381 ctttaggctg gttcttgttg gcctttttta ctctttatgt atcatggagc ttgatttcat
     4441 taagcagctt tctaaagaaa ctttcggtta cttgtacatt gcagccactc gtttatcgca
     4501 aaaaaagtat gcaggttatt ttttccatag caaaaaagag aactttttct ttacttctcc
     4561 ctcgtttaca aatcggcttc ccagcaatct ttgcagaaaa aaacaagaga ttttgattct
     4621 tacaaataaa ccgcaagaag tccaattgct ttggcagcca aaagaaagag gatgccatga
     4681 aacattaact gtcattttta cagcctatga tagtctagga cttttcagaa aaagtgcaaa
     4741 aagagagatc tcttttcctg tgactgttct gcctgctttt tgtaagattc aagcagaaaa
     4801 attacagaga gttctggaaa aaaaacagca gctgaacgcg taccaaagag gtcttgattt
     4861 tcgggaacat cgcgcttatc gtccaggtga ttctttcaac cgaatcgatt ggaaattgtc
     4921 tgctcatact caagagtggt tataccgtga atacgattat caagaagaag aatgttctcc
     4981 tcttttttgc ttttggggtt cgcccagtcc aaaatttgaa cctctgctag acttgtattt
     5041 ttctttatgg catttgaaaa aagaaaagca gccggaactt ctgatattag gcaaccgcgc
     5101 ctttcatggc aaagaccccg gtcataccta ttttgcttct ttggaagctg gcgatgagaa
     5161 agcttttttc acacacttga aaaaatatgc aaaaaaacaa atcgtcctct ttgttcctga
     5221 gaattctcct gaagtctcag aagctattga aacattgtca aaagatcgaa cggtttatct
     5281 tgtttatttc gtagaaaaac aactcatggt ccaagaaaaa gacaagatat gttcattgcc
     5341 aggaggtgaa tggattgacg attaaacggg aaaaatggct gcgtggcgtt tactcctttg
     5401 tgatactaag tgtaggtgct ccgatatttg tagtagctaa tcaattgcaa aacagctacc
     5461 ttcttatcct aactattggt gtcctatgcc ttattgtctg ctgtattcct aaaaaatggg
     5521 tacgtttgct gctttctttg ccagtaatga taggttgttt gtttttatat tttccttcga
     5581 agacattttc catattatgg atcttacagt ttttttcgtc agttacagag gaattcttcc
     5641 atctatccca aggtaaactt tattatttgc cagaaaagac agcttttttt ctcataatgt
     5701 tgctgatcta tttatttatt acattgaccg tggattacga ctattggtac gcaccctttc
     5761 tttctttgat ggcttacttc atgatgctta ccgtttttcg gaaaaaagat ttactgtacg
     5821 aaatgattgc ggtagtaact gttttgttcg tgtatctggc agcaaggcaa tttttctcca
     5881 tccggttgtt gtcgggaaac actcgattcc agtcattgac tacagttctg cttatttgtt
     5941 tcttaacttt tagcgctatc ttgcctttat atcttccgaa agtacaccaa tctctcgagg
     6001 aaacgagtga accgatccga gaatatctta atgatgaagg gctttatgac acactccatg
     6061 agtatcaatt aaccggaagt gcaagaacag gcttcagtga gaatgatgaa cagcttggtg
     6121 gacctttata tgataatggc gaagttgttt ttacagcgaa ccaagaagga aatggctatt
     6181 ggaagatcga aaataaaaat cattacaccg gttctggatg gatagaagat caggacgcac
     6241 ccatccaacc aatccaagaa ttccctgttg acatcacgag ctaccaaacc gttatagaag
     6301 acaaagaaaa cattacattg accctaaaaa aacaagtttt cttaccttat ccttatggaa
     6361 atactacctt gactgatttt tcattagcta gtggacaaac gctttactat ttgcctgaaa
     6421 gccagcgatt cattcctgtc ccgaatgaat cgacacgttc tgcaacttta caaacagaaa
     6481 ggttagtttt tacgagagaa gaattaaaaa tcagtcagtt aaaagctgat gatttagaaa
     6541 atcagcagac aaatcttcaa ttgccaaaaa gtttgcctaa acgtattgag gacttggcgg
     6601 tcgaattgac caaaaatgaa gcaactttaa ttcgtaaagt cgaagctgtc taacagtatt
     6661 taaaaacaag cggaaatttc cgctattcca aaacggatac cgggcatact ccaaaagaac
     6721 acgattatgt ggattatttc ctctttgatt caaaagtcgg ctattgtgac aacttttcaa
     6781 cttctatggt cgtccttctt cgaacacttg gtatcccagc tcgctggaca aaaggtttta
     6841 caaatgggct cagaacaggc acagataatc aagggaatga ggaatacagc attctcaata
     6901 gtcatgctca ttcttggcca gaggtatact ttcctggatt tggctggctt ccatttgaac
     6961 caaccccttc atttgccaat ccaatgggtt cacaaccagt tgctgaaaca accgaaaccg
     7021 tccagcctgc tatcgaacaa ccaaatgaaa gcgaaaatat ggcagaaact atttccagtg
     7081 agagatctga ggtcattcca tcagaagaaa caacactcac accagcttat aaaatcgtct
     7141 tattcagcct tggagtgtgt ttatccgtct tgttgttctt tctattgtta ttcagaaaat
     7201 ctctgcgctg gacaagttat cttatctatc ttcatttttt tgcttctact aatacgattg
     7261 catatcaaaa acttcttaac cctttggaaa cattggtacc gcgcaaaccc gcagaatctt
     7321 tgcaggaata tagcctgcga atagaacaag aattcaagct agatctgcgt ttcattcgat
     7381 taactactca atatgaagca gaaatatatg gacgacaagc agatccggta aaattcgata
     7441 aaaaaaccta tttagaaata acgcatataa taagaaaaat ggtatacaaa aaaattctga
     7501 caaaagggag ccgcttcaaa aaataagata aaatttctgg agaatctact tctggaatgc
     7561 cgagaaccag ctgattgagt atcgattact cgaaaaaaag gttgtgacac gttttttgtc
     7621 acaacctttt ttcattatta tctgattttc aatgtcaaag catttagagc tacaactacc
     7681 gtacttaacg acatcaagac agcaccaact gctggactta atatgatacc aattggcgca
     7741 agaattccgg ctgctagcgg aattgcaaga atattgtaac ctgcccccca ccagaggttc
     7801 tggatcattt tctttcgcgt ttgcttggct agatcaagaa aatgcaaaat atcttttgga
     7861 tcgctgtttg tcaatacgac atccgcagaa tcgattgcga tatctgtccc tgcaccgatt
     7921 gccatcccga ttgaagcacg ggctaaactt ggcgcatcat tgatcccatc acccaccatg
     7981 attacttttt tcccttggtc aaggtatttc tggacaacag cttctttatc atctggtaat
     8041 aaaccgccat aatattcttc gatccctaaa taatctgcta ctgcttgggc agcgttttta
     8101 ttgtcaccag taagcatgac aggagtgatt tttctttctt tcaatgttcg gataaattct
     8161 tttgcttctg tttttactac gtcacctaaa gcaacgattg cagctagatg attttctaaa
     8221 atcaggaagc tgatggtatt tccttgttct tggtaaggtt tcaataatgc ttgttctacg
     8281 tttaatccga gacgctcaac ttctttttca ttaactattt tcacttgttg ttttttgact
     8341 gttgcctcta gcccgactcc tgaaagattt tttaggtttt cagcttgata aggcttgatt
     8401 tcatgttctt tcaaataatt cataatgcca acagccaatg gatgattggc attcgcttcc
     8461 aaggctccta gatattgaag gatttcattt ttattgaacg catcatcaag tacttcgaca
     8521 cccgttacag taaattttcc ttctgttagt gttcctgttt tgtccagcat gatcacatct
     8581 agatcattgg cttgctccag tgcattgcgg tttttcaaaa gtaatccgtt tttagctgct
     8641 attgatgtgg aacgagcaac aactaatggg atagctaaac ctagagcatg aggacaggcg
     8701 atgataaaga ctgttaccat acgttctaat gcattaggaa gatctgtcaa gaacaaccag
     8761 gcgatgaatg ccaggatacc cacaattaat gcgacataaa acagccattt tgccactttg
     8821 tcagataatg actctagctt ggatttttct ccttgtgctt ttcgcaccat ttccataacc
     8881 ttcgctagat aaccattttc accagttcca gttacttcga tttcgatcgt tccatcccca
     8941 ttgatagaac cgccaatcac tttgtcattg gcttgttttt tgattcctct tgattcgcca
     9001 gtcacagctg attcgtcaac aattgtttca cctttcaaga ttacaccatc tgttggcatt
     9061 ttatcaccag accggacgat caaacgatca ccttcgttta cttctttcaa tgaaactgtc
     9121 tcttcttttc catctttcgt caagcgtttc actgattctg gcagtaattc tgctagtttt
     9181 tgtaaagcat tgctggcatt cgacacagca ttcatttcaa tccagtgacc tagtagcata
     9241 ataacgatca aagtagccaa ttcccagaag aaatccatga cgtgtgtatg tggattgagc
     9301 agatttgcga taaacgaata gacactataa atatatgcaa ctgtaatccc catcgcgatc
     9361 agtgtcatca tagcaggact tttttgtttc aattccatct tagctccact taaaaatggc
     9421 tgaccgccat agataaatag tatagttgct aagatcaata cgagccaatc agaaccagga
     9481 aacgttactt gaaatggaaa ttccattccc atcattggtg aaaaaagaat gattgggata
     9541 gctaggataa gcgatagcca aaatttttgt ttgaaattcc ccatgtgcat ggaatgatcc
     9601 atgccgccgt gttcgtgatc catgtgcatc gaatggtcca tttgtttgct gtttgctata
     9661 tctccatggt tatgtcccat gtgcatggaa tgatccatct gactatggtc atgtttcatc
     9721 tccatctgtt tttcatgaga ttctgtactt tctttgttta ctacttcttt tttatgctca
     9781 tgttttttct ccatatcgtt cccattcatt tttactccat cccttctctt actttttcat
     9841 gtgtcgattt aagctcaggg agttcaacaa tacactgacg gaactaaacg ccatcgctcc
     9901 tcctgcaatg attggattca aaaagcctag tgcggcaaaa ggaataccaa tcgtattata
     9961 gacaaaagcc cagaaaagat tttgtttgat ttttctcaat gtcaacctag acaactcgat
    10021 tgtttgtgca atggatgcca aactgctatt catcagcgtc acatcggctg tttccatcgc
    10081 aatgtccgtt ccacttccca ttgccatacc gacatcagct aatgctaagg ccggtgcatc
    10141 attgattcca tcgcctgcca taccgacttt tttgccatct ttctgaagtt tttctacgta
    10201 ggctgcttta tcctctggaa gtacttcagc aaagatatgc tttggatcaa taccaacttg
    10261 tttaccaatc gtttcggctg ccagtttgtt gtctcctgtc agcataaaaa catctaatcc
    10321 tttattctgg agctgcttga ttgcttgttt gacttctagt ttgatttgat cagccacggc
    10381 aatcaacccg atcacttgct tttcatcagc taaaaacatg actgtttttc cctgctgttc
    10441 taattctaac gcgtgttctt ggtattcatc aaatgatagg ttcaattcaa ttaatcgttt
    10501 acgtgtaccc gcaaaatagc gtactccgtt aatcgtaccg ctgattccag cacctgggtg
    10561 ggctgtaaaa tcaatcatat cgtatgttgc tgcttcttgc agttttccgt attccacgat
    10621 tgctttacct agtggatgtt cagaagcttg ttctaaagta taaaaaatag ataggacttc
    10681 tttagaaccg actagatctg taactttggg ctttccttct gtaatcgttc cagttttatc
    10741 caaaacaatg ctgtctaagt gagcagctgc ttctaatgct tctccgcctt taattaaaat
    10801 tccatttcgt gcaccaacac ccgttcctac cataatagct gttggagtcg ctaaacctaa
    10861 tgcacatgga cacgcaatga ctaatacaga gacactatgg atcaatgcga gttgccaatc
    10921 accagtgatc aaacctgtag ctatgagcgt gataaacgcg atgactaata cgatcggaac
    10981 aaaaatccct gagatccgat ctgcgatttt ttggattggg gctttgctcc cttgagcatc
    11041 ttccaccatt tgaatgattt gcgctaatac tgtctgcttg ccgacttgtg taacttccat
    11101 gtgaagtaga ccattcgtat tgattgtccc gccaaagagc gtgtcatccg gattcttttc
    11161 tacaggaaga ttttcaccag taagcatact ttcatctatc gcactgcttc ctgaaataat
    11221 tcgaccatct gctggaatct gctcaccagg acggatgact agttgatcac caacgactac
    11281 ttcttcaatc gctaacgttt cttctttccc gtttcttatc acttgggcgg tttttgtttg
    11341 aagagacatc aattgtttga ttgcattacc tgttttcgtt tttgctgtat gttccaaata
    11401 tttgccgagc aaaatcagcg tgatgatcat actgctgctt tcaaaataaa gatcttgggg
    11461 gtgtccacgg aaaaatccgt tgtaaatgct caatgcaaag gcagccgatg ttccaattgc
    11521 taccaataca tccatatttg gtgctttcgt cttgattgca tggtacgcac ctttataaaa
    11581 acgagcaccg acataaaatt ggacaggagc agacagcact aactggacga ttggaaaatg
    11641 aaaaaagtga acaatcgccg catggcttcc taacatcata gcaatcattg acaacatcaa
    11701 cggcaaagtc aaaaccgtac tcaatataag atcgaatagc atttttttca aatacgcttg
    11761 cttttcttct gcaatttttt gtttgtgggc ttcatcgtac aaaattgcac catagccgat
    11821 gttttctacg ctttggatta atttttcagt ggtcgtccct tcatatttga cacttgcttt
    11881 ttcagtggcc aagttgactg tggcattgac cacaccaggt tgtccgttca gctctttttc
    11941 gatcctagca gaacaatttg cacaagtcat acctgtaatg acgaacgtat ccattttggt
    12001 ttctgctgtt gccatcaaat cacctctgct ttataaccca atccattgat tgcttgacaa
    12061 atcttttcgg attgaacttt tgtttcatca aacttgaccg taccgtttgc ttttttcaaa
    12121 tttactttca ctttttgcac gccgtcaagt gcgcttactg tttcttctac tcttgctgca
    12181 caatgattgc agctcatacc ttcgattgaa aattgttgtt tcattttttc ttcctcctat
    12241 tgatgttgtt tacattcaca ttgacctggg atacagttac attcgatcgt atctactggt
    12301 tgcttttgtt ccagttgttt caaaatctca ttgatatctt cttgagtcaa agtggcttct
    12361 tctactagat ccgcaatcgt ctctccaatc ctttttgcac agatatggga aaaaagattt
    12421 tccgtcgcac tgcgaactgt ttccatctcc gagacagctg gatgataaat gaattttttt
    12481 ccttcttgtt ctgtccagat cacttctttt tttaccaaac gacccagcaa agttttgatc
    12541 gttgctggtt tccaatccat cgaatcagct aagatctgcg tgatttcttg ggctgtcgtc
    12601 tgtccaagtg tccaaataac acgcatgatc tcccactctg aatcactgat ctttactggc
    12661 gttttgactt tttccattta tttcacacct ttcgtttaca aatgtaatca ttatcttatg
    12721 aatacagatt acacttgtaa acaaaaactg tcaattattt ttgctctcta ttttcaattt
    12781 agttaaaaat tcttgtttct ctcgtcttgt tttaaggatg atcagtttat ttcttgcatt
    12841 agctacttgg atcagctgct gtattttcgg ttcattgttt tcttcaaaat gccagataaa
    12901 agataaaaat tctaagtatt ccctgtttag tttctccgaa aatgattcag gcatatctgg
    12961 ccgagatttt ttgtttatct ttctttttag agaacgccaa actactcggt agatggcgag
    13021 ataacgcggc actttaagcc aaatgatctt atctgcttct ttaatccttt cctctaatgt
    13081 agaagtataa tttccttcaa cgatccatga cggattagat accataaaca attgctgttc
    13141 ttgcaaaaac cacttttttg cctgaatact gtagtctgtt gtatgccata aaaggtctag
    13201 cggcagtagc ggaaagtcgg tgatttcttt gatcttcctt gcgagggtcg attttcccga
    13261 cccactttgt ccaatcacga tgatcttcat tcttctccct cacttctctt tggaatttgg
    13321 ttcgatcata ccacttataa cgccgcaaaa aaacaaggga ctgtgacaaa agtcgtgtca
    13381 aagtcccttg tttcgaataa atggtgttca atcagctgac cttcagcatt agctcaaaaa
    13441 gcggcaaaat atgaggagct atttttccgt ttgtttctct aatactcagg tcaaatcgct
    13501 gattttacaa cctcttcctt aaacggtgtt ctcctgccaa cacctcggtc acaagtcaca
    13561 atagtcaaca aacatgagaa gttgtttttt gaaattgctc cttgtgactc ggagttaaac
    13621 ggcaggctca ctacctcttt cttaaacggt gttccaaagc ttgcatctgc ggtaataagc
    13681 ccagccaaac gaaaaatatg aggggctatt tttgtttggc tgttcttatt acttgatgca
    13741 ggacagcttt ttcacaacct ctgattcacg gtgttccaga agtagcttct tcgaaaaaca
    13801 agccgaaact tcacaaaaat ttgagaagca attttcgtaa attccttctt atttcttgaa
    13861 gctgaccact tctgtcacaa cctcttgtaa caagtatttc tattttaagg ctgaaccact
    13921 gtttctggtt cagtgatttc tttgtttttg tctcgacgag caattgctgg caagatcata
    13981 cctaataaga tcaagacgat tggtgtgatg atattcgtaa ataattggaa ccaccatgct
    14041 ttaggatcag cagtataatc tacttttggc accattccta ggatacatgc aaacgcagtg
    14101 aaggcaaagc accaagcacc aaagataaaa ccgatttttg gatttttaac aaatttatat
    14161 tcagaattga atttcttata agctttattc aacatcatat atgccaagaa cacccatagg
    14221 taacgcattg gcatgacaac tgagttcaag ttcgtcaacc attttactaa ttcattcata
    14281 tctttgatac caaaaatcgg caacaaaata atgattccta ctagtatacc tgtcagaaga
    14341 taaccattca ccaatgtacc tttacgatta cgtttacgta gccagtttgg tacaaattct
    14401 ggatccgcat ctgctaacaa gatttttagt ggtgcatcaa ttgaaaaggc taatgcggag
    14461 atttgtccaa tcatgtttgt caatgcgtaa atgatcatca atgagttacc gataccatag
    14521 taattaccta aaatttgaaa agcactgtat gccccatttg ccatcaagtc tttaggaata
    14581 ttatcactag caaacagcat acccattgcg actgagccta atacagcaca aatccctacc
    14641 attgctgcta agaagatcat ccctttagga aattcctttg caggatttct tgtagagttg
    14701 acataagggg atattttttc cgcccctcca actgcaaata ctagcatcga aacggttgtg
    14761 aaataactga aatcaaatgt tggaatatat gttttcactt gattcatatg tggtgtggca
    14821 aattccatcg ttggattgat gaacggtgcc gttacagcta aaagaataaa taagatcgac
    14881 atgacgaaca tcgctgttcc ggccagtccg ccaatcactt tcaaagtaga aagtcctttt
    14941 gttgataaat ataaaaaggc aagaaatata ataagtgaaa taactgctac catttgtact
    15001 gacatcgtat ttacgatatt tccatttcct tggactgccc aaccaccagc aatcagaatt
    15061 gcttgaggct tttgtgccaa ataaggaata tggacgaccc agtaagtcca agcggcatag
    15121 tatgccagtc gtttattaga tgtcgtattt tcgacccatg aactgacgcc tcctttacta
    15181 tccttgaagg ctgatcctaa ttgtccaacg atcaatgcgt atggaataaa ataaatggcc
    15241 aaaatcaata gccaagaggt gacaactgat actccttgct gagcatagtt attaactaca
    15301 ttccccatgc cccaaaccat gttaaatgca attaacgcaa cagtaaacca gcgtaattgt
    15361 ttgttgttca ttcgttctct cctttatgat aaaaatttgt tatccagatg taatcatatt
    15421 caatttttaa caaacttaca agacagtgtt attttatgtg tgtattcttt tatttaatac
    15481 acatcgtttt ttcatatttt ttccatcaaa aaaataagtt attttctttt atttcattga
    15541 ccttattgaa tttcaagcta ttttgaattt tctgcaaaat taaaaacatt agttatttta
    15601 taatcctatc tattttcttt acatttttca ttaagaaaag gatataaaaa aaagaggacg
    15661 ttcattcgtc ctcttcattg atcaacgttt attttttaca tgcaagcttc gcatggcatt
    15721 caaaacagct aatactgtca ccccaacatc tgcaaatact gcatctccca ttgatgcaaa
    15781 accaaaagca cctaaggcaa gtaccaaaat ttttatccca atagcaaaga caatgttttg
    15841 tttgacgatt ttcaatgttt tctttgctaa atggattgct tctgcaatct tcgatggttg
    15901 atcattcatg atgactacat ctgctgcttc aattgcagca tcgcttccca atccacccat
    15961 tgcgattcct acatctgctc ttgctagcac cggtgcatcg ttgattccat ctccgacaaa
    16021 tgcggtcttt ttattgtttc tttgtaagat ttcctctaac cgctgcactt tatcttcagg
    16081 aagcaattcg ctatatatct tgtccacgcc tacttgcttc ccgatatgat cagcaatttt
    16141 tttcgagtcg cccgtcaaca tgactgtgtt cttcacacca gcctgtttta attgaaccaa
    16201 tgcgtctacg gcatcttctt tcaaggtatc tgcaataaca agataaccac tgtaactttg
    16261 atcgatcgct agataaagca atgtaccgat ttcttgactt aaaggaaaag agatttcaaa
    16321 tctttccatc aatttgtgat tacctgctaa tacttgtttg ccatcaactt cagctaagat
    16381 cccttcacct gcaaattcct ggatattagt tgttggtaaa agcggacctg cgtagccttc
    16441 taaaacagat tgagcaatcg gatgtgtaga aaactgttct gcactcgcaa tatattgcat
    16501 gaatttcttt ggatcgattt ttgtatcgat cgtctggatc gaaaagtcac ctttcgttaa
    16561 tgtgccggtt ttatcgaata ccactgtctc ggtttgcgca agcaactcca agtaatttga
    16621 accttttact aatacaccga ttttacttgc gcctccgata ccgccaaaga aactaagtgg
    16681 cacggaaatc actaatgcac atggacatga gatcactaaa aatgtcaatg ctcgatagat
    16741 ccattcagaa aacgccgctc cagaaacaac aagtggaggt aaaacagcta ataaaacggc
    16801 caatcctaca acgactggtg tgtaatagcg cgcgaattta gtaatgaaat tttcagctgg
    16861 tgctttttta ctgctggcat tttctactaa ttctaataat ttgctgatag tagaattttc
    16921 ataagatttt tctacttgaa ctaaaaatgg tttattttta ttgataaatc cgcttaatac
    16981 tgtttcacca acatatacag atttagggac tgattctcct gtaagtgctg acgtatcgac
    17041 taatgattct ccttcgatga tcgttccatc taacggtaca cgttcaccag gcttcacaag
    17101 tacaaactga ccgatctgca cgtcttcagg gttcacggtc atcaatcctt ccgtcgtctg
    17161 cacgtgagct gtttctggac ggatcgccat caattcgcga atcgattttc ttgaatgact
    17221 gactgccaat ccttggaaat actcgccgat ttgataaaat agcatcactg ctacggcttc
    17281 tggatattca ccgatcaaca tagcgccaat cgtagcaaca gacataagaa aattctcatc
    17341 aaagattttt cctttgacga tattcatcac tgccttcttc actacatcat atcctatcag
    17401 cagataaatc aataaatacc ctatcaagcg aattggttca ttaaaatcaa ataatagcaa
    17461 taaagcgata cctgcaaggg aaaataaaat ccggaacagc gtatttctct ctttttcatt
    17521 tccatgttca gatacttttt ccttatctat ttctgttact ttgacatctg gttctaattc
    17581 cttcactact ttttttgttt cttgttcaat cgtagccaaa tgatcttcct ctgcgtcaat
    17641 cgttagtttt ccagaagtga agtttaccgt tgcttccttg actccattga tcttctgaac
    17701 tcctttttcg attttcattg cacagttcgc acaatctaaa ccttctaatc tgtaactttt
    17761 cattttaatg acctcctcgc ctcaacatat gaatatctct tcataactaa ttataaatga
    17821 atggttattc atatgtcaag gtatccgaaa gaaaaaatga aaaacatctg catcggaaaa
    17881 tttcttccat acagatgttt tggcttattc tttctttttc tcttcgatca ttgcccctgc
    17941 tcttatccaa cccgcacttg ccttcttgat cttgacagga tagcaactaa gtttgaagcc
    18001 tgttgctggc aatttgtcta aattcattaa tttttcgata ggacaatatc ttttttcaat
    18061 ccctgcaaaa tgtccttccc agatattcga agctccacta cgctttcaaa aatatagaaa
    18121 gagttcttta atgaaaatta tttcaacttt ccataaacag taatcccata taaataaaag
    18181 acgtaaaagt cacatgggag aacttataca ggaaaacaca tagagtcagg tgaaatacat
    18241 gtatcccacc tgactctatg cgttttctaa cctttattat tctcttttta tacaatttct
    18301 gcaccgaatg gcatcaacgc caatcgagca agtttgaaag attgcttggc aaaaggtatc
    18361 cctatgatcg tgatacatag aaggatgccg ctgacgacat gtgcgagagc aattggtagg
    18421 cctgaaacga tcagccagat gatattaacg atcaatgaca tgccagactg gtgataaatt
    18481 acttctttgc caaaaggcgc aaaagaaagg gaggcgatct taaaacattg cataccgatt
    18541 gggataccta tgatcgtgat acaccaaagt atccccgctg ctaaccagct aagaccaccg
    18601 attagtccgc cgaatataaa ccagatgaca tttcctaaac aactcattgt atttctcctt
    18661 ctattactgt ttttgattaa aactgggcaa aaattgattt tattcttctt tttttagctt
    18721 ccagcatcaa gtattctgca aatttttttt ggatttcttc cgtattttct tttgttacta
    18781 atgtgtgttg gattagtttt cctgatggtc gctgatccac ccgttcttct gactgttgca
    18841 cgtccgcttg cgcaaatccg ataatccgtt tttcttttgt tacagcaacg agcaatagtt
    18901 tattcttatc atttaggtca gctgccgttt ttgcttttac gaatgattct ggatgatcag
    18961 taatcagctg attgatttga ggtaaatctt cttttactgc ccaccgtatt tccatcattt
    19021 ctcacttttc tttcgctcat ttttatctcc actaaaaaaa tcgactgcgg caattgcatc
    19081 tatccagctt tgtgcaatga tctcatgtcc gccaatcgtt gggtggaccc catcttctct
    19141 tgtatatttc gaaaaaccat aagcaatccc taaagcattg aatatgccat ctaaaggaac
    19201 cagcaatgcg ccatattcct tagcaagttt acgaacaatt tggattttag ggtctaagtc
    19261 tttacgccag ccatgacggt cgtcggttat aggaagtaca aacggctcca tcaagatgat
    19321 gttgcagatt tccgctgttt gaagttcatt tagtgatttt cgataaacgg attcaaattg
    19381 ctgcgcagat ttctcagttc cgaatatagc ctgatcgcta gtattatgcc aggtatcatt
    19441 gattccgatc aaaatcgata catagtcagg ttgtaaagct aaacaatctt cttgccagcg
    19501 tacttgcaga tctgcaattt tgtctcctcc tatcccacgg ttcatgcatt caaattccat
    19561 cgttggatat tgggagatta gttttccggc aatcaaaagt ggataacctt tacctagatc
    19621 agttgcttct gctcggtctc ttcctgcgtc tgtaatgctg tcaccgataa ataatatacg
    19681 ttttttcatt tgtcaaaaac tcctttttga ataaaacgat gattttcttc cttatattat
    19741 cccataagcc ctatcaagac gaaagagaaa atatatgtat aaaaaatgac accagcctag
    19801 tcccttcaat caattgaatg aacgctcatc aagtgtctag ttttttatac tttttttctt
    19861 aacgtgccgg attccccttg attccttcgt tgtttggtcc agagaaatcg atcacaccta
    19921 agcttccgtc aatcagacca ctgatgacgc catcagaaca tatatttata ggctcatcta
    19981 agacttccag taaatgttct tttactaata tctcttctag attgcttggg atagattggt
    20041 cataacttta tctggtccga tgatttcaag gatgaattaa acttacatat ggatcattgc
    20101 atgctgaaaa tacggaccta ctccatcttc gaatgttact attagtttgt tttcaggtac
    20161 tagatgctgc tttatttcaa cagcagaagc atcatcaaat cagatttcca tttgaataac
    20221 cttctttgta tatgtttgat tgattgcgat ttcgttatag aaccttgtga atcaaatagc
    20281 aatttctttt gctttcttct cttgtctgct ggaaacttcc tttatcccgt attttaaaaa
    20341 aagaactgcg agagttcgca gttctttttt ttaaataaac gttgctctcc ggccaactcc
    20401 tcggtcacaa gtcacaatag tcatcaaaca tgaaaaactg ttttttgaaa ttactccttg
    20461 tgactcggag ctgaacggca ggcgcacaac ctcttgatat aaacggtgtt caatcagctg
    20521 accctcgata ttagctaaaa aaacggtaaa atatgagaag ctatttttcc gttttgtttc
    20581 tctaatactc aggtcaaggc actgatttca caacctcttc cttaaacggt gttcaaaagc
    20641 cagtttctcg gtattaagcc aaatttatgc aaaaatgtgg aaagccattt ccacaaaatt
    20701 tttcttaata ctcgaaacta aacagctttt tcatcacctc ttatggataa attcggttca
    20761 acaaacgtgg gaatggaata gcttcacgta catggtcgat accagcaatc caagtgactg
    20821 ttcgttcaag gcctagacca aaaccagagt gcggaacaga accgtattta cgtagatcaa
    20881 gataccatga atattctttt tcatccaaac cgtgattacg gatttgttct agcaagaaat
    20941 catagtctgt tgcccgttcg cttccgccaa taatttcacc gtaaccttct ggtgcgatca
    21001 tatctgcaca aattaccacg tcttctcttg ttggatgtgg tttcatgtag aatggcttga
    21061 tggcttttgg atagtttagg ataaagacag gctgttcgaa tgaattcgca atgaacgttt
    21121 catgaggtga accaaagtct tcgccccatt cgatgtcatc aaatccattt ttcttcagta
    21181 attcgattgc atcatcgtaa gtaatacgag ggaatggcaa ttttgtataa gtttttaaga
    21241 cttctttgtc tcgtcctagt acatctaatg catattcgca atgatctaaa acattttgta
    21301 ctaagaatgc tacatattgt tcctgtactt ctaaactttc ttcttgatga gtgaaagcca
    21361 tttctggttc gatcatccag aattcgatca aatgacgtcg tgtttttgat ttttcagcac
    21421 ggaaagtcgg accgaatgaa aagacttttc cgaaagccat agcagcagct tccatgtata
    21481 attggccact ttgagataaa taagcttttt gatcaaagta atttgtttcg aatagatccg
    21541 tagtgccttc tggtgcagaa ccagtcaaga tcggcggatc gattttgaca aaatgatgtt
    21601 gattaaagaa ttcatacgtt gcacgaatga tttcatttct gatctgcata atcgcatgtt
    21661 gtcttgaaga acgcagccat aaatgacgat gatccatcaa gaaatctgtt ccgtgttctt
    21721 ttggtgtgat cggataatcg tggctctcgc ttaccacttc cacatttgta attccgattt
    21781 cataaccaaa ttttgaacga ctgtcttcac ggatttctcc agttactaac acggatgttt
    21841 cttggtgtag atttttagct gtttggaaaa cttcttccga cacttcgctt tttacaacta
    21901 caccttggaa aaaggctgtt ccgtctctta attgtaaaaa tgcgattttt ccgcttgatc
    21961 gcttatttgc gacccaagcg ccgattgtta ctgtttctcc cacatggttt ttcgaatcga
    22021 tgatttgaat ttgttccacg tatgactctc cttttatatt ccttgaattt cttcaggatt
    22081 aattttgact tttactctca ataaattgct tgattctgcg taccgcttct tctaacgtag
    22141 cgcgatcagt ggcataactc atgcggacat tttctggtgc cccaaagcct tcgccagtca
    22201 caagcgctac ccctgtttct tctaaaagat cttctaccca ttgtgtgaca ttttcatact
    22261 tacacatgcg tagcgtttct tttacattag gaaaaagata gaaagctcct tgcggctttt
    22321 caagagaaac acccggcagt tctgccacaa gcggatataa cgtattcagg cgttcttcaa
    22381 aggcgttccg catttcttct actgtatctt gctcaccttt caatgcttca acagctgcat
    22441 attgactgac tgctgtcgga ttgctagttg attgggaagc aacagcgatc attccattga
    22501 tgatcacagg atttcccacc gcataaccaa tgcgccagcc tgtcatcgca tatgttttag
    22561 acacgccgtt gatgatgatg gtctgatttt tgattgcttc tgaaatcgtt gcaattggag
    22621 taaactcatt gccattataa actaaacggc cataaatatc atcagcaact atcaaaatat
    22681 cattttttac tgcccattca ccgattgcct gcagttcatt cttgctataa atcattccag
    22741 tcggattcga tggagaattg atgattacgg cttttgtttt ttctgaacgt gcttgttcta
    22801 gctgttcaac agtaactttc cagctattgg attcttcccc tttcacaaag acaggaagtc
    22861 cttctgccag ttttacttgc tcaccgtaac tgacccagta aggcacagga atgatgactt
    22921 catcttgcgg gtcaaggatc gtttggaaaa gggtatacaa agcaaatttc gctccgtctg
    22981 ttacgactgt ttgtgaaggt tcatagacaa gtccatagtt tttcttcaga taatcaacga
    23041 ttgcctgtcg aagctctggg attcctgctg ttggtgtata atagctagct ttcccattgc
    23101 ggattgcttc gattgctgct tcttggatgt tttctggtgt cgcaaaatcc ggttctccca
    23161 cagtcaaact cagcacatct ctgccttttg cttttaatgc cttggccttt gccgcagctg
    23221 ccaaagtgac agacggttct aatcgttttg ttctttttga taactccatc ccattcattc
    23281 ctttaccttt taaattccct tgattgtctt gacttcatct ccagattcat agccaatcag
    23341 gtaataattc agtttcccat ttgtttcttt tcctactact tcccacacag gctgatcatc
    23401 atacatccct aaagcagctt tttcgatttg gatttcagga tgggccgcag caattgtctg
    23461 ttttgcttca ttttcagtca gtccctttgt ttgatccaat acttttactt tttcaccaga
    23521 tttaggaatg ataacggcga tttcttttcc tttatcattt gtacctgtca gtgaaaagta
    23581 tgtcttcttt cgagtaaacc aataaaaatg atcggcttcc tcaagatttg catatttctt
    23641 tgcgatttct actgcttctt tcttcgcctg caccgccgga cgactggaac gcaagtaaaa
    23701 aatcgagctg atcacgatga ccgctaataa aaatacgatc aagccaagta acacgcgatt
    23761 aatcgttttt tcctttgtct gttttttctt caagggtcgt cgctccttta tcttcattcc
    23821 ttttcattat agcagatttt aattatgaaa aaagacagct cctctaactt ccttcttcat
    23881 ttttctttaa gaattcatgg atatcctgat agatttcctc catagctagc tctttgatcg
    23941 gtactttttt cggtaatgca tggatgatcc gtgcaccata ttttgcagta actagtcttc
    24001 gatccaaaat aagcaatacg ccacgatctg tctccgtcct tatcaagcgt cctaatgctt
    24061 ggcgtaactt caaagttgct ttaggtacgg attcctggta aaaaggattg atcccttctg
    24121 attctaaata agcgttccta gctttgacca tcgggcgaag cggattatcg aagggtaaac
    24181 gtgtgactac aacgatctgc aaagcatctc caggcaaatc tacaccttcc caaaaactgt
    24241 cagcgccaaa caatacgctt ttttctgaca aaatgaagcg tttcaaaagc ttctcccggc
    24301 ttccgccaaa cccttgagct agtatttctc gtcctttttc tagcatagac agatgcattt
    24361 tctgatagac cttctgcaaa acatcatgag aagtgaataa cacaagtatt ggccgatctt
    24421 cttgttcgat cagacctgta agtgctgtct ctaaataatc gatatatgct tgattgctga
    24481 cttgatggac ggcaggtgcc tcttttggca catagatcct tgcctgtttc tcataatcat
    24541 aaggagaggc aatcacttta agaggtgctg cttcaatgcc aagcctctta gagagatagt
    24601 gtcgattagg gccgatcttc agcgttcctc caatataaag aatacgatca tatcgaggat
    24661 accaaacagt cgacggcaac atagccgctt gaaaatcatg gagatgaaaa atcatctgct
    24721 gcgctgtgtt cccaggaacg aaccaatgga tcaaatgatc tcgccagtct tccagccatt
    24781 gatccatcag caagtactgc ttgtctaatt gatcaaatag gctatataaa cttgctaaat
    24841 cgatccgcat ctggtttgtc cagctttctt ggataatctc aaaagtcaga cgcatttgtg
    24901 actgtagctg ctggatctcc tgataaaaaa gctgcaggtg atcaatggct gtcccagatt
    24961 ccagtggcag ctgatccaac atctcttttg tcatcatgac ttcttctgga tactgtccgt
    25021 tgggttgctt gccgctcact aaactcatta atccttctgc catacaaatc tgtgcttcgc
    25081 atagcgcaga aagttcttct cgataaatcg ataaccagcg taaggcttca tgttctttcg
    25141 gtaaatagct ttgtattcgg tcaaacagct gatttggttc ttctaattgc tggatttgtt
    25201 tatggaaata gttaatactt aatcgtttgt catttacttt ttcagcgatt tccggcaaat
    25261 gatgtacttc atcgatcaac aaaaatggac tagctggaag caatggttct tttcgctgtg
    25321 tttcttgtac taaaaacgca tgattgacaa tcaaaaaatt actttgcctg attttctttt
    25381 gcaagaaacg aacaaagtct tgttcgtaaa atgactgatt tcttgctaaa aatccaatac
    25441 ctctgtgcgc gatatctgca aagaatgaat ggttcaaatt tgttaaatgt aattcatcca
    25501 aatcaccggt cttcgtttgc gtcagccaaa ctaagaccgc catttgatac aaagcatatt
    25561 gtttttgttc tggcggttcc acaagtgttc ctttaaatcg ttgcaggtcg atatagtggc
    25621 gatagctttt gataattaca ggttgtatgg gctgatctag caattgattc agcaatggaa
    25681 tgtctttttc tatgatctgc tgctgcaaaa ctagcgaaac ggtactgatg acagcgggtt
    25741 tttcaggtgt agccaaataa ctcaatggta gcagataacc gattgtttta cccattcctg
    25801 tagctgcttc gatcatcaaa tcttttgatt catcttttgt gaaatggtcg tagaccgcat
    25861 tcatcagtct gttctgttct tttcgataca tcagtgtttt accaaacatt ttttcttttg
    25921 cttgtttttt tcttggatat gtccgctcgc cataatggac ggatgtgaat agttccactt
    25981 cttttttctg taaagctagc ccatcgacga tttctaacga gggatcaagt ggctctggtt
    26041 tttctttcat ttcttcaatc acatgataaa tgaaacgact cgtatccata cctgtgtgtc
    26101 tacttaatcg cgcaatctgc tctaacgtga tgattggcaa ttttctcatc cgttcttcga
    26161 tcagcaaaag aagttgacct gtcacttcgg catcactatc tgcctgatga ggattctcat
    26221 gaataaatcc aaggctttct gacaaatcag ctaagcgaaa gcttggttcc gtcggcaaga
    26281 aaatttgtgc aagttctact gtatcgatgc ctggtatctt caaactagga agtccgcatc
    26341 gcatgagttc atggttcaaa aaattataat caaaataaat attatgcgcg acaaatatgg
    26401 tgtctgccaa aagattgtaa atcgtttggg cgacatcttc gaaataaggc gctttttgga
    26461 cgcgttgatt cgtgatccct gttaaatgct ggatttgttt tgaaatcttc cgccctggat
    26521 gaatatcgat cgaaaaacgt gaagtgatcc gacctgattc aatcatgacg cagccaaatt
    26581 ggatgatccg atcttctttt ggattcgtcc cagtcgtttc gatatcaacg actgcataag
    26641 ttcttggttt caaaatatct tcctcttctc ctgcctctaa tgttcttttg aaattatact
    26701 acaaagtgca gaaaaatact ctcatcgctt tttaaagaaa atagacaaaa acgcccagac
    26761 acgcaaatgt ctgggcgttt attttttagt ataggttaat taagctttag taacgtttgt
    26821 tgcttgaggt ccgcgttggc cttcttcaac atcgaaagtt actgcttggc cttcttctaa
    26881 agttttgaat ccgtcgcctt ggatagctga gaaatgaacg aatacatcgt ttccgttttc
    26941 tgcagtgatg aatccaaatc ctttttctga gttaaaccat tttactgtac ctgtttccat
    27001 aataaaaatc ctccttgtgt atcaacacat attttgcaat tacttgaatg gtgtatatct
    27061 ggaagatgtt tatttgaaac aagctaccac agattaccga acaaaaacta ctcctctagt
    27121 atatcatacc gtttacaaaa aagctagagt ttttcgactt tttttattaa aaaaagtttt
    27181 ctcagcgatt acataaagcg aaaaaccgaa ctttttttaa aataaacgac atattgttca
    27241 gttggtgttt ttttgctctg atgttctttt ttaaactaaa aaaattattc tttctgacta
    27301 cttatcggtt ctgtttcaac ggtcgaggct ggggaagatt ctttagtagg tctgatgttc
    27361 tcatggtatt tctgatagcc tatgacagtt accagaagaa caaggcaaac caatacgatc
    27421 aacgagatta gtttttcttt tttgctcata tctatacctc ataaataaca ggatgatgtc
    27481 catcatattt ctgagtatga gtaaaagcaa aagctgcagc tgtagaatgt aagatatgcc
    27541 agcaactttc ataatcacag gtcggacagt accaaaagta accaaatctt ggctgcaaag
    27601 accgttcttc tgtttcagga atcgcagaat caagatctgt tgtctgtaca taaacatttt
    27661 tgtacagacg tgtgcctgta ataggagacc gctgtgtttc ttcagcaaaa actggctgac
    27721 ttgttaaact tagggataga aacagcaaac accataacag tctttttttc ataaagacct
    27781 ccttatcgat aagtttttcc caattaaaat catatctcaa gaacgcccgt tattcacatt
    27841 ttgttcataa atgatgtttt ttgtatcata atcgtcaaaa agaggtcgtg accacgtcac
    27901 aacctctttt ttaaacggtg ttccagaagt agcttcttcg gaaataagcc gaaatttcac
    27961 aaaaatttga gaaataattt ttgtcaatct cttcttattt cttgaagctg accacttctg
    28021 tcacaacctc ttccttaaac ggtgttcaaa agcttgcatc tgcggtaata agcccaccaa
    28081 acaaaaaata tgaagagcta tttttgcttg ctgttcttat tacttgatgc agaacagctt
    28141 tttcacaacc tctcatacac ggtgtttaat cagctgacct tcggtattag ctcaaaaaac
    28201 ggaaaaatat gtgaaactat ttttccgttt gtttctctaa tactcaggtc aaaacactga
    28261 tttcacaacc tcttttaata acgaagaatc gctgtcagac tcttttcatc tggcgcatct
    28321 ttttgatcca aaataaacac tttcccgcca ttaatgatca catcatctgc taatgcattc
    28381 aacacttgac gccgatcata ttctgtttca ggatcttcac cgaaaccatc agctaagttt
    28441 gacgtagcga taaataaatg tgacactttt ccttcttttg cagctggaac gatatcaact
    28501 aactgatcga tgaatttctt atctaagagt ttattgtatt cttttgattc ttgagctgcg
    28561 agttcacttg acagcttctt cgccccttct tcgatatctc taaaggatag ctgcgcagga
    28621 gaagcagaaa tcgcaacgtc tgcacaatag taaggatttt tggcgatttt tttgaacatc
    28681 gtctgatttt ctggtagagc aaataaataa atcggccatt gttctggatt ttcgaaagaa
    28741 tccttcaagt acgtatcaac tgcttggtaa tagttgaccc aatcgatctc tacttcttca
    28801 tcttttggat ttacgccatg gaaagttact ccatctcggg ctccgcctcc tccattagaa
    28861 gagaagttca aaccgccacc tgtcagttca gatcccaatg cagtaacgac atcaaccggt
    28921 gccccttctg gtagagatac ttcttttact tgtttgttgc tgacttcata caatttcatg
    28981 gagtcgcggt tcaatcccaa taaataataa gaatagttga actgcccatt tttgataata
    29041 gccagtaaat aaggaagatc acttacatag tattgatcgt ctaccgggat actcaaacga
    29101 tggatatagg tttggtcagc agttataatg accgctacac tcgtagttgc actacgccaa
    29161 aacgaaggat ctcctaacaa attttcaatt ttttcttgga acggggacca gtcatgttcg
    29221 gaatattttt tctcaaaacg tttctttgct tctttcgaaa aatttttgaa tttgatctga
    29281 tctttttcca catcttgatg ggcgacatgc gtattcagca tgatcgtgac gaatggtcct
    29341 ttagcgcctt ctgaaatcaa ttgtgacaat aattctttat ccttttttgc cataaacgta
    29401 cccctcctaa aaaaatcatt cctttgtttc tatctaaagt ctaccacaac actatttttc
    29461 catacaataa aacgtatcta aaaaaataaa acggtttcta cggttttaaa tttagctatt
    29521 tttttaagtt atcttcataa tttcctcaat tatttagccc cagtcttcaa ctattcttca
    29581 tgaaatgctg ataaactaag aaaaagaata aaagaggtgt atcttatgca tttgattatg
    29641 attttcagtt tattaagtat tatcagttta tttgctttca gcgcttcccg cttattagag
    29701 gcggaaacac agcctgttcc tataaaagta cgaaaaaatg accgctaacc ttttcataac
    29761 gcgcgttctt atcctaaagt ttttttcaga aacattgaaa ctctcttcct tctctgataa
    29821 aattttaatt tatagcgaaa caaattaaat acaaggagat cggatatgtc cataaaaaaa
    29881 acacgtctag tcctattatt ggctagtctc attctttgcc ttgcttcttt tctaccagct
    29941 gttgctgtgc aagctgaaga tacgttcaaa gtaaatgcaa aagcagcttt tgctgtggat
    30001 gcgcaaagcg gtaagattct ttatgatcaa gatggagaaa aaaccatggg gatcgcttca
    30061 atcacaaaaa tcatcggtct ttacatcgtc cttgatcaag taaaagaagg aaaactatca
    30121 tgggatgata aagtttcgat ttcagattat gcagaaaatt tgagtatcac cccagattta
    30181 tcgaatgttc ctttgcataa agaaaacacc tacactgtca aagaattatt cgactctgca
    30241 atcatccagt cagccaatgc ttcaatggtg gccttggcag aaaaagtctc tggcagcgaa
    30301 gctaaattca ctgaacgaat gaaagaacaa ttgaaagatt ggggaatcaa agatgcaacg
    30361 atcgttaatg catcaggatt gaacaactct tatcttggcg aaaaccgacc agaaggcact
    30421 ggagaaaacg acgaaaatca aatgtctgct caagatgtgg ccatcgttgc ccgccatttg
    30481 atcttggatt tcccagaaat actggatgta tcaagtacta cgacccaaat gtttggcgaa
    30541 aatacgcaat caccagtgga aatggtcaac tggaactgga tgctcccagg attcgttaat
    30601 tacaaggaag gcgtggacgg attaaaaact ggtacgactg agcttgcagg tgcttgtttc
    30661 gttggtacga tcactaaaaa cgatcaacgg atcatcactg tcgtattgaa tgcggacggc
    30721 catgcggaaa acccgagtgc ccgcttcaat gaaacagacc gtttgatgga ctactgctat
    30781 gacaattggt cagaaaaaga gctcggaaaa gctaacgcaa gtattcctag cctaaaaacc
    30841 attgatgtaa aagacggaaa agaatcaaca gttcctgttg tgcttaaaag ccctgtaaaa
    30901 gtatgggtac gaaacgatat ggatactggc aaattaacga tcacgccaac acttgacaag
    30961 aagcaagtca ctgacaacga gatccaagca cctatggata aaggtacaaa aatcggtacc
    31021 gctacgatca ccttggcaga cgataaatta ggttatttag aagataacaa tgcgccatcg
    31081 acagagatca ttacggataa aacagtggaa aaagcgaata tgttcgtgat tggctggcga
    31141 cgcgtcgcag aatttttcgg caacttgttt taattgaaca ataagaaaag catctttcca
    31201 tcggaaagat gtttttttgc tttttctagt ttaataacag cttttttcat ctattttcgt
    31261 tatactgtat aagaaaaagg agttgaattt atgaatctaa ccagagatct gatcttttta
    31321 gttctctatc ttattggggt attctctatc gtttattggc gaaaacagaa gaaggatcct
    31381 tccttgttct tttacggcag tacgtggaca tttacggtaa tcttaactta ttttgtcgtg
    31441 ttggaaattt tttattcgac cttttatttt ttgattccat tgttgttctt tagtatcttt
    31501 gctttttctt atttcacaga aaaacgaagg ctgctgaatg gtcttttatt caatgttttc
    31561 ctcatcagtt tcggcatcta cttgtttgtc ttgctgtatg aaacgcaaaa tattttcctt
    31621 ggcggactga tcgccttgat cacgattcca ttgctacttg tccttttatt tggtatttat
    31681 ggattgattg tttttctttt ttggaacggg gtcacagttt tacgtagaga atcgcattca
    31741 ttagctaatc ttttaacgtt gatactagcc atttttctta cactgtttct tgtttttgac
    31801 tttttcttgt taaaatattt gccgcaatgg ataaatgctt tgtttttctg cgttcccctg
    31861 atcttgatct atctatttat cgtattctac aactttttga ctgtctcatt cttgtatcag
    31921 ttcaaccggc ctcgatacaa tcaagacttc atcgttgtgt taggcgctgg gttgatcaat
    31981 ggagaaacag tttctcctct tctagccaag cgaatcaata aggcgattgc tttttatcgg
    32041 gctcaaagcc gtgctacatt aaatccgccc atcttattga tgtctggcgg gcaaggagca
    32101 gacgaaaaag taccagaggc cattgcgatg aaacaatatg ccatggaaca aggaatccct
    32161 gaaagagaca ttcttgtcga aaccaattcg acaaccactt tggagaacat gctctattca
    32221 aaggaaatca tggatcagca aatgaaaggg ccataccgcg tgatctttag ttccaacaat
    32281 taccacattt ttagagccgg tctttatgcg tgtcaagcca aattgaaagc aaatggtatt
    32341 ggtgcaaaaa cagctttcta ctacttgcct aatgcgtttt taagagaata catcgccatc
    32401 ttgatgatga acaaaaaaag acatatgatc atttgcggat tgcttttcgc aggctccgca
    32461 ttcttatcgc tgattacttt acttttctaa aaaaacaagg tgtgacaaaa catatgtcac
    32521 accttgtttt tttctttcat tctcttaact attggcaaaa agagtatccc ttttttgacc
    32581 gctacttgct gagcagaata aataccgctg ttgcctgaac acataaagct gatcaagcag
    32641 atcataaaga agtatactgc agcttgtgaa ccgaacaatt cgatccccat cacaaagcag
    32701 gcaatcggtg tatttgtcgc accagaaaat actccgataa agcccaatcc tgcaagaaaa
    32761 ggaacagaaa catggagcaa aggcgctagt gctgcaccaa gcgttgcacc aatttcaaac
    32821 aatggtgtca cttctccccc ttgatatccc gcgcctaaag acaaaactgt gaagaataac
    32881 ttcccaataa aatcatagac atgtgcctgt ccattaaatg attcttgaag taacggaaga
    32941 ctcaatccga gataacgttg tgtttgcaaa atcaatgctg ctgccacaac tacagctcca
    33001 cctatcgtat ttcgccaaac aggatgagga atccattgtg catatcgagc ttttaaaaag
    33061 acgatcgaac ggctgaatac ccagccaatc aaaccaaaag caatcccagc aacaaaaact
    33121 ttgaagaaca gcaacatact ccattcagga atcacaccca tttggtaatg tagatgagaa
    33181 acaccatagc tttctgtcat gaaatttgca aacagtgcag caaagaagct agggaagatc
    33241 gcttctgttc ggacttttcc gattgccaac acttctaatc caaataatgt tccagctaaa
    33301 ggtgtgccaa atacagaact aaaacccgca ctgattccac tgataatcaa gatttcccgt
    33361 tctgatttat ccaatcggaa caaacgtgct gtattatctg ctaacgctcc ccccatttgg
    33421 actgctgttc cttcacggcc aacagatccc ccaaataaat gggtagttat ggttccaaac
    33481 agtgtaagtg gaataagtcg caacgggatt ttttcttctc caccatttcc ttgatcgatc
    33541 actaagttgt ttccacgact agagagaccg ccgtgataca catagagata agcaaaaagc
    33601 gcaccactaa ctggtagtag ataaaggagc caaggatgtg atagtcgggt atctgtcaca
    33661 actgtcaagc tatttaagaa aaaagcagat aagctcccca tgaatatacc gagaatcgct
    33721 gtgatcacca gccacttcag catatacact aaaatgcgca ttggactcgg taattgtttt
    33781 tccataaaaa attcctcctc aataaaaaaa cgcctactaa agaaaggggc tgtccccaga
    33841 ctttagtagg cgtcattagc tatctttcgt tagcatttaa aggcgaacac catcacctat
    33901 tcacttccat taaaagatag catatttttc tgtaaaaaac aataaaatta tcgttctttc
    33961 tttacacgtt cagcaaattc atagaaagga gccaatttcg ttaactgttg attacattgg
    34021 tcactatcat gacaaatata attccccttt ttcgtatacg tgccatcaga acctgctttc
    34081 gttgtagaaa gaaaaagcga aacattcgat gttttctgac agattgcaca gattccctta
    34141 tttatcgttg gtgataaagt accactaatc ccttttaatt tttcatcttc gtaatagacc
    34201 atgaattttt tctgcgtacc tggatcattc cagccgatgt acgtatgttc ttgcaagtcg
    34261 atttcattcc aagctggttg tttgagtttt ttcacttttc ggaataattt ctcgatttgt
    34321 ggtttgctag gctgcataaa cggctgaacg atttgcttca gctcctccaa tgctctttct
    34381 gcttgcgctt ttgtcagttt tacgtctaaa actgtcgtta aaaatgtatc gatttctagc
    34441 agttcgtgat ctaatatgtc tcgaatcttt tcagctgcta gtgcctgaac tgtttgtacc
    34501 gtttttggat cattgacaga tgagtacgca tttaataact gttctacttc tctttttata
    34561 aaaaaatatt ggtatggttt tattgtttgt tccatgattt taaagtcctt tcttctaacc
    34621 aaagtgcata ctttgtagaa aaaagtgatc ttctgcaaag tatgtgttac ctgaagatcc
    34681 cggactcttt taaataagat acgttttgca tcttatcatc acccccttta atgcttattc
    34741 ttctatgtac atgattcatt cgataaataa aagtaaaccg tatcttgaac agatagtcaa
    34801 ctgcaaacag aaaaaaggac tgtgacaaaa gtcgtgtcaa agtcccttgt ttcgaatgaa
    34861 tggtgttcaa aagcttgcat ctgcggtaat aagccgaaac ttcacaaaaa tttgagaagt
    34921 aattttcgta aattccttct tatttcttga agctgaccac ttctgtcaca acctcaatat
    34981 ggtctattat tggttttacc tgttaaatcg aggattcttt cccaaagtaa cgttgccatc
    35041 tcttacgcat acttgcagac atgggctgag ccgcttcttt gactgcgcag tatttgtcca
    35101 cgaatgtcac gatatatcct tctttgtact taggcggtgc gatcgttgca ccccacatgt
    35161 gcttcaaaat gatatctcgc tcaagatccg acaaatcggt gattttttct gcattcttta
    35221 cagcaattct tggatggata tacgcatgtg ttccctcgtc aaatttcgtc gtacgccaat
    35281 catagtagaa cagatcatgc aaaagtccag cacgcgctgt tgctttagca ttgccacccc
    35341 atttttttgc gagcagataa ctatagtaag aaacactgat ggaatgctcc aaacgagtgg
    35401 aatgaacatg ctgtgtgtaa ttcgctaatt ttttcactgc ttctgtttct aaaagatctt
    35461 gtacataaga catatattct tcgtcttcac gccatttttc tgtcataatt atcgatctgc
    35521 cacctttttg aatttcattt tcttctgaaa ttgatctaag tataccacca gtgttcggat
    35581 tattctagtc gctctagata aaatagctaa agcttaatag ttccttaatc ataaagaaaa
    35641 atgataaaga acgatccctg ctgcaactcc gacattcaat gattccgcat ttcctttcat
    35701 tgggatatat acgttctgat cagtcaaaga caagatttcc tgacttactc cctgaccttc
    35761 gtttcccatg attatagcga aatcctctgt tttttctact ttgtctaatg catgtgcttc
    35821 ttcatttaat tctgtaccgt acaccgtaaa ccctttgcct ttcattacag ggattacatc
    35881 tgccaaatct ttttgtaaca caggcaaatg ataattgctt ccctgcatgc ttcgcagcac
    35941 ctttgtacta taaatatctg ccgttccttt acctaataaa acaccagcca taccaaacgc
    36001 atctgccgta cggatcatcg ttcccacatt tccaggatcc tgtacattat ctagtaaaag
    36061 ccagcctcct gaaaaatctg gcgtatcttc agatgttaac gtcatcactg ctaagattcc
    36121 ttgcggtgta ggtagatctg acatagcggt catgacctca tctgaaacta gaaaagtaga
    36181 aatctctttg tttacagcca gccagtctcc ccattctttc atacctctct gtgtcacaaa
    36241 aagttctttt atttctgcat cagacgacgc agcttcttcg atcagatgga acccttccaa
    36301 aagatattct gccttttctt ctctgtattt ctttttatgt agttttttta attctttgat
    36361 tcttggattt tttgctgatt ggatctcttt cacattcctc acctcaactg attatatcat
    36421 ggaataggtg cttgagcttg tttcatccta aaaaaatcgg tatcattgat ttataaaaca
    36481 ggaaagaaag gtgacgaaca tgcgcaaagt taaaatgaat gtccaaggac gtgtccaggg
    36541 cgtgggattt cgctatatga ccaagatggt tgctgatcag ttaggcgtga ccggaacggt
    36601 aaaaaacgaa gacgacggct cagtcacaat cgaagcaatc ggaaatgacg acatcatcca
    36661 aaaatttatc gaagaagtca agaagtctcc gagcccaagt ggacgagtgc agtatgtgga
    36721 tgtccaagaa gatccgatga ttgaggaacg aaagaaattt gatgtggtgg gataaggttg
    36781 tttttgaagc gctttgcctc gagtattaag gagaaacatt ttcaaaatag cttctcatat
    36841 tttgaaatgt aatgacttaa taccgagagg cagctgataa aacaccgtca ataaggttgc
    36901 gaaaaagctg atctgcatca agcattaagg actggttgcg aaaaagtcgc ttagttttga
    36961 gtattaagga aaattttgtg aaaatggctt tccacatttt tgcataaatt tgtcttaata
    37021 ccaagaaact gacttttgaa caccgtcgat aaggttgttt ttgaggcgct ttgcctcaag
    37081 tattaaagac aggtagtaaa aaagctgatc tatcacttgc agtgagttca tttgactcct
    37141 ctatttcggg cagaataaaa aagtttttag ttttttaaat aagaaagaaa cacttggaat
    37201 cactcttttc tttccaagta tttctttctt gattttttgt cttttttcaa aatttttcgc
    37261 tacgaaaaag aaagaatatt tatatgaaat ttctatgaag ttgattgaat tccatgcgct
    37321 tttttagtat agttaagttt gtgtaaaaat tatagaagag gaagtattct atgaataaat
    37381 ggaaaaactg gttattggga tccggacttg tcagtatcct gctcttttta tctggctgtg
    37441 tgcggatgga ttcaaacgga aaccctgaca cttcaggaat cgtttatcga gtactcgtcc
    37501 atcctatggg ccaagcgatt acatatctag ttgagaattt caattggtct tacggctggg
    37561 cagttattgc aatgactgtg atcgttcgta tcatcatctt gccactaggt atcagtcaat
    37621 cgaaaaaaac aatgatccaa tctgaaaaaa tgcaagcgtt aaaaccacaa gttgaagcag
    37681 cacaacaaaa gctaaaagct gcaacaacac gtgaagaaca aatggcagca caagcagaaa
    37741 tgcaacaagt atatcgcgaa aacggattga gtatgaccgg cggaatcggc tgtctgcctc
    37801 tattgatcca aatgccgatc ttctctgcct tgtattttac ggctcgctac acagaaggta
    37861 tccgtgaatc atctttttac ggcatcgatc taggctcacc aagtatggtg cttgttgcca
    37921 ttgcaggggt cgcttatctg cttcaaggat atatttcaac aatcggtatt cctgaagaac
    37981 agaaaaagac gatgcgtacg atgctgatcg tctctcctgc tatgatcgtc ttcatgtcga
    38041 tcagtgcgcc agccggtgtt acactttact gggtagtcgg tggtgtgttc agctgtctgc
    38101 aaacattcat cacaaacgtt atgatgaaac cacgtttgaa agcacaagtg gcagaagagt
    38161 tgaaacaaaa cccgccaaaa caagtcgtca ctccaagaaa agacgtgaca gaatcagcaa
    38221 agccagctga caacaaacaa gcaacaaaac aaatcaaaca accaaaaaac agtacaaaca
    38281 acaaaggccg caacgcagga aagcagcaga agcggaagta gatagtaaat aagagtgtaa
    38341 taaaaattca ttaacagttt tagcaattaa aaatatcgac caagattttt gaaaaagaaa
    38401 tctggtcgat atttttattt aagtgaatgt ttaatttgaa tcatcgattg atcgctatct
    38461 aattgcagca gcatcttttt atgaagaaac aactttttct tatctatcaa cgtagacagg
    38521 tgttgctgtt tcttttaata atgtacttaa ttccttaata ttttgttttc cttcttctaa
    38581 catccaacta ctcgcttttt tttcgccttc ggtaacaaaa ggtgccactc ccttcgccca
    38641 tgttgctcgc ccacataaga taccattgaa tgtcgaacct gattgttttg caaaacgtaa
    38701 tgtatcttga aatagcgtag cattgactcc cgcacttaaa aaaataaacg gttgttcagt
    38761 cactttcgat tgttcataga aatacgcgga tgcctctgct tgactataac aagtctcttg
    38821 cccatatcct tccacaaatc tcatattcac tggcacttct actttcaata catccacttg
    38881 ataacgttct tttgaaaatt ctcgcatggc ttcaataact ttatggggtt ttacttttgc
    38941 ataggcaaga gaagacggat cgccaatagt tgtatcataa gaaaggatct ctaaaaagaa
    39001 aggtatgcct tctgcttcgc attctgaacc aatccgttct ataactattt gtttttgcac
    39061 attaatctct gctggttcat ccacgtcata gtacaataaa aatttacaag catccgcacc
    39121 agcttctttt aaccttttca ccgaccaaat attcaataaa tcaggtaatc ttcccggcat
    39181 tttcgtatca tagccggttt tttcataagc tagcagcaag ccacattctt tctctcgaag
    39241 cgctgctgca ggcaggccgt attctggatc aagtaaaata gctgaggcat agggagtcag
    39301 gtattgagaa accatctgtt taaattggat gatgtcttct gatgtagctt ctttatattg
    39361 gttgatcatt ttcttcaaag caccacgctg gtcgattgct aacgcgctaa taattccagc
    39421 ttcatccgtc aatcgcttca aacgttcctg cttttctata ctcatcttct tcatatatct
    39481 tcacctattt ctttagttaa tattgtggct atttttgttt ttgattttgc ttttctcaac
    39541 tgttctgtaa cctcttttcg catcaatgat cgtgcaacga gagacaataa tttcaaatga
    39601 gtcgttcctg cttctgaatc tggaataaat aacgcaaaaa taaaatagta aatatctact
    39661 cgtgaaaacg ggtggttttt ggcagcccta gaaggcctca tcacttgctt atcctcttaa
    39721 gaggggcttt ttaagggacc accaacttgt accgaccccc aaaagttaga ccaaaaaatc
    39781 taacttttgg aggtcggtat tttttatggc taagtatact tttgaactta aattaaagat
    39841 cgttcatgat tatttggatg gcaaaggcgg aagtgattat ctggcaaaga agtattccat
    39901 taaggctcca tcacaagtga agcgctggat caatgcctat caagaatttg gtgaagaggg
    39961 tctagtaaga aagcgacaaa agaaaaaata ttctgttcaa tttaagctgg atgcgataga
    40021 gttatatcta acaagtgagc tgtcctatcg tgaagttgcc aacacactta acatgaacaa
    40081 ccctaatttg atcgctagtt ggatgcgtca attccgtgag ggtgggatcg atggactctc
    40141 aaaaacgaaa ggatgtccac ctatcttgtc taagaaaaat gaacccaaaa ataagaaaaa
    40201 atctacaact aaagcaacgt ctaaagaacg cgaacgcatc gaggagttgg agaaacgagt
    40261 ccgttccctt caaattgaaa atgccttttt aaaagaattg aggaagctgc gaaaacagga
    40321 agcccaacaa cgacgaatga agcaatcgca cgaatcatcg caagcctccg aggaccgttt
    40381 aaattagttg agcttctaga aactttaaag ttccctaagg caacctttat gtactggcaa
    40441 aaacgattgg accgtaaaaa tccagatcaa gagattgaag cggaaatgct taaaattcgg
    40501 gagaagcaca aggattatgg ctgtttacgg atgaccaatg aactaagaaa ccgcggtttc
    40561 tctatcaata aaaagaaagt tcagcgcctg atcaagaagc taggaataaa agtaacggca
    40621 tacacacgta aatcacgtcg ctacaattct tatcgaggac aagttggagc agtagccaaa
    40681 aatcggatcc atcgtcgttt ctacaccagt atctgccatc agaaaatcac gacggataca
    40741 actgaattta aataccatga agcggatgcc aaaggcatta tccgtcaaaa gaaattgtat
    40801 ctagatcctt ttatggatat gttcaatagt gagattttat cctatcgtat ctctgaaaag
    40861 cccaatgcat tagcggtaat ggaaggtttg gaggaagctg tccagaccac taacgattgt
    40921 ccctatcgtc gtatgtttca cttagatcaa ggttgggcgt atcaaatgaa agcttataga
    40981 aacaaattga aggagtacaa aatctttcaa agcatgtctc ggaaagggaa ctgcttagat
    41041 aattcactta tggagaattt ttttggctta ttaaaacaag agatttttca cgggaaagtt
    41101 tataactgtt tcgaggaatt aaaatcagcc attgatagct atatctatta ctacaataat
    41161 gaacggataa aacagaaatt aaactggcaa agtcttgtcc aa
//
LOCUS       DACDIG010000020        39790 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.020, whole genome shotgun sequence.
ACCESSION   DACDIG010000020 DACDIG010000000
VERSION     DACDIG010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.020"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..830)
                     /locus_tag="IV247_001092"
     CDS             complement(<1..830)
                     /locus_tag="IV247_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8777174.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     "
     gene            complement(1054..2994)
                     /gene="thrS"
                     /locus_tag="IV247_001093"
     CDS             complement(1054..2994)
                     /gene="thrS"
                     /locus_tag="IV247_001093"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAP8777175.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3331..3510)
                     /locus_tag="IV247_001094"
     CDS             complement(3331..3510)
                     /locus_tag="IV247_001094"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAP8777176.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3557..5380)
                     /gene="cadA"
                     /locus_tag="IV247_001095"
     CDS             complement(3557..5380)
                     /gene="cadA"
                     /locus_tag="IV247_001095"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP8777177.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5658..7937
                     /locus_tag="IV247_001096"
     CDS             5658..7937
                     /locus_tag="IV247_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8777178.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8030..8677)
                     /locus_tag="IV247_001097"
     CDS             complement(8030..8677)
                     /locus_tag="IV247_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777179.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8856..9575)
                     /locus_tag="IV247_001098"
     CDS             complement(8856..9575)
                     /locus_tag="IV247_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP8777180.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9706..10857)
                     /locus_tag="IV247_001099"
     CDS             complement(9706..10857)
                     /locus_tag="IV247_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAP8777181.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11074..11814)
                     /locus_tag="IV247_001100"
     CDS             complement(11074..11814)
                     /locus_tag="IV247_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8777182.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11818..12141)
                     /gene="rsfS"
                     /locus_tag="IV247_001101"
     CDS             complement(11818..12141)
                     /gene="rsfS"
                     /locus_tag="IV247_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAP8777183.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12198..12791)
                     /locus_tag="IV247_001102"
     CDS             complement(12198..12791)
                     /locus_tag="IV247_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8777184.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12775..13422)
                     /locus_tag="IV247_001103"
     CDS             complement(12775..13422)
                     /locus_tag="IV247_001103"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAP8777185.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13435..13746)
                     /gene="yhbY"
                     /locus_tag="IV247_001104"
     CDS             complement(13435..13746)
                     /gene="yhbY"
                     /locus_tag="IV247_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAP8777186.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13762..14871)
                     /gene="yqeH"
                     /locus_tag="IV247_001105"
     CDS             complement(13762..14871)
                     /gene="yqeH"
                     /locus_tag="IV247_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAP8777187.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14868..15395)
                     /locus_tag="IV247_001106"
     CDS             complement(14868..15395)
                     /locus_tag="IV247_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAP8777188.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15575..16360)
                     /locus_tag="IV247_001107"
     CDS             complement(15575..16360)
                     /locus_tag="IV247_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAP8777189.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16353..17222)
                     /locus_tag="IV247_001108"
     CDS             complement(16353..17222)
                     /locus_tag="IV247_001108"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAP8777190.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17223..18602)
                     /locus_tag="IV247_001109"
     CDS             complement(17223..18602)
                     /locus_tag="IV247_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAP8777191.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18610..19029)
                     /gene="fabZ"
                     /locus_tag="IV247_001110"
     CDS             complement(18610..19029)
                     /gene="fabZ"
                     /locus_tag="IV247_001110"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP8777192.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19054..19530)
                     /gene="accB"
                     /locus_tag="IV247_001111"
     CDS             complement(19054..19530)
                     /gene="accB"
                     /locus_tag="IV247_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAP8777193.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVISEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19533..20768)
                     /gene="fabF"
                     /locus_tag="IV247_001112"
     CDS             complement(19533..20768)
                     /gene="fabF"
                     /locus_tag="IV247_001112"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAP8777194.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20787..21524)
                     /gene="fabG"
                     /locus_tag="IV247_001113"
     CDS             complement(20787..21524)
                     /gene="fabG"
                     /locus_tag="IV247_001113"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAP8777195.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21536..22453)
                     /gene="fabD"
                     /locus_tag="IV247_001114"
     CDS             complement(21536..22453)
                     /gene="fabD"
                     /locus_tag="IV247_001114"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP8777196.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEATPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22560..22784)
                     /locus_tag="IV247_001115"
     CDS             complement(22560..22784)
                     /locus_tag="IV247_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP8777197.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22837..23799)
                     /locus_tag="IV247_001116"
     CDS             complement(22837..23799)
                     /locus_tag="IV247_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAP8777198.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23809..24249)
                     /locus_tag="IV247_001117"
     CDS             complement(23809..24249)
                     /locus_tag="IV247_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8777199.1"
                     /translation="MESDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24555..25520
                     /locus_tag="IV247_001118"
     CDS             24555..25520
                     /locus_tag="IV247_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP8777200.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25577..25807)
                     /locus_tag="IV247_001119"
     CDS             complement(25577..25807)
                     /locus_tag="IV247_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAP8777201.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(25893..26780)
                     /locus_tag="IV247_001120"
     CDS             complement(25893..26780)
                     /locus_tag="IV247_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP8777202.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(26869..28092)
                     /locus_tag="IV247_001121"
     CDS             complement(26869..28092)
                     /locus_tag="IV247_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP8777203.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28109..29188)
                     /locus_tag="IV247_001122"
     CDS             complement(28109..29188)
                     /locus_tag="IV247_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP8777204.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            complement(29319..31046)
                     /gene="ptsP"
                     /locus_tag="IV247_001123"
     CDS             complement(29319..31046)
                     /gene="ptsP"
                     /locus_tag="IV247_001123"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAP8777205.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(31046..31312)
                     /locus_tag="IV247_001124"
     CDS             complement(31046..31312)
                     /locus_tag="IV247_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAP8777206.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            31791..34025
                     /locus_tag="IV247_001125"
     CDS             31791..34025
                     /locus_tag="IV247_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP8777207.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            34173..34493
                     /locus_tag="IV247_001126"
     CDS             34173..34493
                     /locus_tag="IV247_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP8777208.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(34698..34922)
                     /locus_tag="IV247_001127"
     CDS             complement(34698..34922)
                     /locus_tag="IV247_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HAP8777209.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(35120..36700)
                     /locus_tag="IV247_001128"
     CDS             complement(35120..36700)
                     /locus_tag="IV247_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAP8777210.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(37101..38477)
                     /locus_tag="IV247_001129"
     CDS             complement(37101..38477)
                     /locus_tag="IV247_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP8777211.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(38605..39723)
                     /locus_tag="IV247_001130"
     CDS             complement(38605..39723)
                     /locus_tag="IV247_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8777212.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
BASE COUNT        11286 a         8567 c         6878 g        13059 t
ORIGIN      
        1 tattcttgga ttgcttctcg tgtttttttc actttttcgc taagcttttc gtttttagcc
       61 atatagaaat aaaaaatgat atcgatcgta gcaaattggg ccaaaagtga attagttgcc
      121 gcacttcgat tcgtgatttc ttttggtcta gatgtttgta cgagtacatc ggctatcttg
      181 ctaagcgggt tatttccaaa ttgcgtcaac gcaatgattt cgatattcat gcttttcgcc
      241 agctcggcta atgcgactac atctgcactt ctacctgagt ttgaaacaag ccaaagcact
      301 ttctttttct cattgctgac aagttgcggg agcaagacgt ggatatcttt ttcaaaaatg
      361 attggcttac cgacacgaga ccatttttgc agaatatctt ctactactaa agaagaggcg
      421 ccaaccccat ataaaaaaac agtctctgcc gattccaaac actctacgac tttttggatc
      481 gtttcttctt ctagaatatc tactgtttct tttatcgcta cttgtgcatt gttaccaagt
      541 ttgtttttga tagagtaaaa ggactcattt gcttctatat cactaaaacc ttcatcgatt
      601 ccagtctgta gttctgttga aatcgtgatt ttgaagtccg tcagactttg aaaaccgatt
      661 tttttggaaa aacgtacaac tgtcggcgca ctcaacccgg aagcttctgc taattcatta
      721 gctgtcattg tagggacctt ctcaatatta ccgataatgt agttggccaa ctttttttct
      781 gctgaagaat aatcttccat catactttca atacgactaa ttacgctcat aagttcctcc
      841 acccttttaa tgatagtcta tgtaagtata ccatatcaga cccttattca cggtcgtata
      901 ctggataaaa aacaaaaaaa caatccgccc aacagaatat cttatcccat caatcccaca
      961 gattctattg agcatctgtg tctttgagga atacctgtta tctattgttc ggattgcttt
     1021 ctatctacta cttttttaca ggaaatttac ctattagttt tctctgctgt aatttttgac
     1081 ttctgcttcc atagcatcaa taaagatatt caaatcttct acagctgttt ctttacttcc
     1141 ataacgacgt acgttcactg ttgcatcttc caattccttg tctccaacaa ctaattggta
     1201 agggatcttt tgtgtttgag aggcacgtat cttatagccc attttttcat tacgatcatc
     1261 aacttctaca cgcaacccct tcatttgcaa acgttctttg atttcataag cataatcgcc
     1321 atgtgcatca acagaaacag ggatgattgt tgcttgtata ggtgccaacc atgttggaaa
     1381 agcgcctttg tatacttctg ttaaataagc gacaaaacgt tccattgtag aaacaattcc
     1441 gcggtggata actactggac gatgcgtatt ttccccgtct tctccaacat atgtcaaatc
     1501 aaagcgctct ggcaataaga agtctaattg tatagttgat aatgtttctt ctgtaccaag
     1561 agctgttttg acttgaacat ctagttttgg tccgtaaaag gctgcctcgc cttctgcttc
     1621 aaagtaatct agttctaatt catccatagc ggctttcagc attgcttggg ctttttccca
     1681 cattgcgtca tcatcaaaat atttatctgt attgtttgga tcgcgatagc tcaagcggaa
     1741 acgataatct gtaatattga agtcagcata tactgccacc atcaattcaa gcgtacgttt
     1801 aaattcatct ttgatttgat caggacgaac aaatgtatga ccatcattca acgtcatttc
     1861 tcgtacacgt tgtaaacctg acaaagcacc tgatttttca tagcgatgca tcattcctaa
     1921 ttcagcgata cgaattggta attcacgata agaatggatc gtgtttttgt agaccatcat
     1981 gtgatgaggg cagttcattg ggcgaagaac aagcatttcg ccatctccca tatccattgg
     2041 cgggaacatg tcttcttgat aatgatccca gtgtccagat gttttgtaca actcaacatc
     2101 tcccattatt ggtgtatata catgttggta acctagacta acttctttgt ctacgatata
     2161 acgttcgatc gtgcgtcgga ttgttgcccc ttttggaagc cagaatggca aaccagaacc
     2221 tacttctggg gaaaccatga acaagtcaag ttcttttcct agtttacgat ggtctctttc
     2281 ttttgcttct tcacggagac ggatatattc tttcaaagct ttcttgtcaa aaaaggctgt
     2341 cccgtataca cgttgcatca tttgattgtt tgagtttcca cgccaataag cacctgccac
     2401 tgataataat ttaaatactt gtatacggcc agtggatgga acatgtggtc cacgacacaa
     2461 atcgatgaat tcgccttgtt gataagcagt aatcacttct tcttctggca attcactaat
     2521 cagttctact ttgtacggat cacttgcaaa aatttctaat gcttcctgtt ttgaaagaac
     2581 acggcgttcg atcggcagat tttctttgac gattttcatc atttctgctt cgattgccgg
     2641 caaatcttct gcagttactt ggttttgtcc attatctgta tcgtagtaaa accctgaatc
     2701 gatagctggt ccaacgccaa agtggatatt tggatagagg cgacgcatgg cttgggccat
     2761 caaatgagct gctgaatgac ggactaagcc aagtgcatct tcatggtctg gtgtgatgat
     2821 ctcaatactt gcatcttcct cgattggacg agtcaaatca acgagttttc cgttcacttt
     2881 accagctaaa gctttttttg ctaagctttt tgaaatgctt tcagcaattg ccaatgttgt
     2941 tgttccagac tcaaattctt tgactgcacc atctggaaaa gtaattttta tcataatagt
     3001 ttcctccttg aaatgttttg attgatcaga tcatcaagct gaaaaacaaa aaaatcctag
     3061 catctcttga aaagagatac taggacgaaa agttcgtggt tccacctaat gttcaaacgc
     3121 aaatgcgttc ctcaagcgtt gtaacggtcc gaccgccctt gcttcaacaa gggtttcgaa
     3181 agtggtcatc ctttggtttt ctcctggaaa agtttcagcc tagcttttcc tctcttatcg
     3241 aagactctca aattcagttg gctttctcat caatcaatta tgtgtttatt taaccactaa
     3301 taaatagaaa aatcaagtgg gaaactaaac ttattttttc cattccccat ttacaccgta
     3361 tgttcctttt ttcatctcat tgatgatgac atggatgtgt tcttttggtg cctgcgtgtt
     3421 tttatgaaca gcttccgtga tatctttcat catattttct aattgttccg gactgcgacc
     3481 ttctactaat tcgacatgta cgaatggcat aatgctctct cctctttctg atcgttcttt
     3541 tttcaactcg attctatcat actatgtttg tcttgggtac tgaatcagga aaaatggctg
     3601 gatctttttt tgctctcaag gcactcaaaa tagagaccgt atccacgact tcttgcaaaa
     3661 cagctccgaa taatgcgggg attagtcccg tacttgcaat taacatcaaa aaaatacaaa
     3721 taaagatgcc aatcagtaca gactgtttcg ctattctcat cgtatctctt gaaatcacta
     3781 ctgcttttgc tacacgcaag agatcatctt tcaagatgac cgcatctgca ctttcactag
     3841 cagctgtaga accatgagcc cccattgcaa taccgatatc tgcagcagca agtgctggtg
     3901 cgtcattcac gccgtctcct accatcgcaa caggtctttg atctttaggg atactttcta
     3961 aaatggtcag tttatcttga ggcaagctac gggcatgtac agaatctaat tggacttttt
     4021 ctgcaatcgt ttgagcaatg gcagatccat ccccggtgat catcatcaaa tttttcacat
     4081 caagttttct tagctccttg attgtctttg ctgcttctgg gcgaatctga tccgaaaata
     4141 ggatcgttcc tgcaaaagat cgatcaattg ttacatagat tgctgtattg gacgtttcta
     4201 tttcttcagc tgaaacaaaa gaggcacgtc caatctttac ttcatgatta ttgactactg
     4261 cttggattcc ttgaccggtc acttctttca gttgactaac aggatacagt tggctgtttt
     4321 tcacgttgtt cactaacgaa cgagctaaga tatggctaga ttcctgttct gcactagctg
     4381 catacagaag tagctcttct ttttttattc cattgactgg ctgaatcgcc tctacttcca
     4441 atatcccttt ggtaagcgtt cctgttttat caaaagcaat cgtttttgta cgtgcgagct
     4501 tttcaatcgt tgttccagtt tttacaacga tcccgttttt acttgaacgg ctcattcctg
     4561 caactaaagc aatgggcgca gctagaatca gtggacaagg tgatgccact accagtacct
     4621 cagcaaatcg taaagggtct tttgagaacc accaagaaat accaccgatc aaataagcaa
     4681 tcaacgtgaa agggactgca taacgatcag ctaaacggac aaatcgggca ggcttttctt
     4741 ttgattcttc gactagttta ataatcattt gatactggct gtctgcggca cgtttttcag
     4801 cgatcatttg gatcgatccg tctccattta aagaccctga cataatctga tctccaggtt
     4861 tcttggtttc cgggcgggac tcacctgtca aagaagattc atcgaccgaa gattctcctt
     4921 tggtcactcg accatctgcg ggtacaagtt cgccaggctt gactagtaac tggtcatcaa
     4981 tatttatttc atcgattact atatcctgca attttccttc tttgaatcga tgggcaattc
     5041 ttggggtgtt atctaacaaa gactgcagtt ctttacccgc tcgtttattc gcgtaatcct
     5101 ctagactatc tccgcccgta agcatgatca atatcatcaa actagcccag tattctttga
     5161 cggctaatgt tgcaaggatt gcggtaatcg ctaatacatc tacgccatat cttcctgaac
     5221 gtaacgtctt gatcatttcc aaaaacatca agaaggcgag aatccctcca gtaaatgcga
     5281 caatcgcaaa tgcaagggcc ggctggtgaa aaacaaattc acttaataaa gctatcccac
     5341 caataagcaa agtaagtaaa aattttttga aatgtgtcat tgttttttct cctcttttat
     5401 gtgacttaga gaggttagta ataaatttcc agctgcctct ttcatcttaa gcatagtcct
     5461 tgactagcac tttttaaaac aataggcctt aattgaaaac aactatcact atcagcgttt
     5521 ccaaaattca tcagttctac aaacaaaaat attcagcaag gattactttt tcgattcatc
     5581 agttaaaaag tacctttttt cgaaaaacta tgctatactt ttaattcgtt atttacggcg
     5641 gaaggagcaa acatgcgatg aatgagaaac aactagaaca aacgcacgtg gatgaaacga
     5701 tcgaactaat ccagatcgaa caaaatatgc tggatacaaa acaacaagaa ttgactcagg
     5761 aaatgaatca ttcgacaaaa gacacagcta atcacaagat tcgagctggt tccaatgaag
     5821 ctttttatga atctgctgtt gaatatcgcc aacatgagca ggaattgctt ttgaaatacc
     5881 atactcttga agcacagcaa aaacgcctgc aaacgttgtc tgtcatgaaa gatagtcctt
     5941 attttgcccg tatcgacttt aaagaagacc gtgaaacaga aacattatat ctaggtatcg
     6001 cttctttaag agatctggca gaagaaacaa tcgttattga ctggcgcgca ccaattgcca
     6061 atctttatta tgaaggtgag attggtcccg cattttatga aactgaaaca gatagaatag
     6121 atgtcgatct gcagttgaaa cgacaattta aaatccaaga aggacgtatt ttatctatgg
     6181 tagatacgtc ggaagtaatc aatgatgact ttttgctaga gatattggat gaatcatcaa
     6241 gcaaccatat gaaaaacatc gtctctacga tacaaaaagc acaaaatgca atcattcggg
     6301 acacaacaag taaagtaatg ctgattgaag gaatcgccgg aagtggaaag acatccgctt
     6361 tgcttcaacg tattgctttt ctgttgtatc ataatcgcaa gtggctcgag gctgaaaacg
     6421 ttcttctatt ttcacccaac catttgtttt ctgattacat ctcgactgta ttgccttcat
     6481 taggtgaaag cggcgtgcct acacagacat tcaaaaatta tttggtgcag ctgctgccag
     6541 agttttcttt actagatgaa cagcaacaag aaagtggttt tttgctaggg gaaaaagatc
     6601 cgatacaagt gatgaaaagc ggattgacct tagtcgatca gattgatcgc tatatccaag
     6661 gcatcacaaa ctacggtccc ttgttccgtg atatgaaaat aaatggccgt acgatcctat
     6721 caaaggaatc gattcgccaa tggtataaag aaacaaatga acaattacct ttacatcata
     6781 gactttctct attacaaacg aagctcctga aaaaattagg cggtctgcaa aaagacgaaa
     6841 cacgtcagca atgggtcaaa gatctggcgg aagaacagct ccaagaacta tacgcaacag
     6901 atccaaatct tgaatataca gaacaaaagg agcgttcatt acgtaaaaaa ctggccaacc
     6961 aaatcgtacg taaaagattc cgtcgaatcc atcgagggat catgaattat caatttgtca
     7021 atataacgaa acaatatctc cactttctac aaactgtgcc actttctctt ctaacacgac
     7081 ataaaatcag cgagaccagg tggcaggaac atttgacaac tgttcgtaca cacttgcgtc
     7141 aaagagaatt gcgtcaagag gatgctgtac tattcttttt actgatgcgc cgaacatatc
     7201 cagtgacggt tacacacaaa gctcgctata tctttattga tgaaatgcag gatttcgcac
     7261 catcacaagt ggcattgctg caagccttgt tcccaaaagc aaatttgact ttctgtggag
     7321 atctaaatca aaatgtcttt ggaaatgaaa cgatcgttgg atcattggaa gaccttttcg
     7381 tggaccgtga agtcactcgt ttccaattaa cgacaagcta tcgctccaca aaagaaatca
     7441 ctgattttgc taatcatttt attgtgaaag aacatcaagt agaaacaaca gcaagaaaag
     7501 gagaccttcc tttgctcatc caaggaacaa cagatgatat caaattgaat tggttagcag
     7561 ataccattcg aacgactcag ggaaaagccc ggtattggcg tacagcaatc atagctaaaa
     7621 cacaagaaga atgcgaagct ctttatgctc aactgccaga cgagttgaaa caacaagtcc
     7681 agctgatttt aaatgagagt gactttatga agcgttccat tattttactt ccagcctttt
     7741 tagcaaaagg tctggagttt gaccgagttt tcttgtggga tgtagatgca gaacattttt
     7801 cttctcagca agacaaattg atcctttaca ccatgtgtac acgtgcgatg catgaattga
     7861 ccctttttac ctccaataat gaaagtcctt tgttaaacaa tatggatatt tcatcttatg
     7921 agaaaaaagt actgtaagta aaaagacaac tgctatcatt aatgttagca gttgtctttt
     7981 tatttacaat aataattgca gaaaaagtaa ttcaatcatt taaggtcatt tattctatat
     8041 ataaatccaa atctgtttca ggatatttta atttgacaag cgaaataatc attttcagtt
     8101 cttccatgtt ttctggatat tctgggtcaa ataccttgct atgacggcca atcccgccat
     8161 tgtcgatcaa tcggtaaatt cgattccatt caatgaattg gtcaattgat tggaaattgc
     8221 tgattggata gccgccacaa tccaaaatct ctgatacacg aaatgctagg tatgcactat
     8281 cgatcacttt attttttttc gtgtctatct tctcatttct ttcgataaga attttttcag
     8341 gacgatgttt caagctccac tctaatagta gaaagaaagc ctccatccga tactcttctt
     8401 caaataaccg aaaggctgaa tcagaacgac gaaaaagcag cggaatcaga taattttgtc
     8461 tataaatcag cattggtgcg cggtccacca tacactctaa ataacggttc agataccctg
     8521 taccgacata gtttcctaat cgatagcctt caacgccgaa tcttgcgtat ttttgcgcta
     8581 attttgaaca atcagttacg ttcacagcta actcttctac tttgcggtaa ccgcttaaga
     8641 tacgttcaaa aggttctaaa gtgatttttg cttccatatt cttccccgtc catcttccac
     8701 atacttcata tcatttcaca tgatacttat gctctcatca taccacaggt tgaattagaa
     8761 aagaaaataa atcacacaaa aaagcaagac aatctgttag tgagattgtc ttgcttttcg
     8821 aaaaagactg aatacttttc gttaaaactg gttgcttagt cttctaatgc cacattatgg
     8881 aacacatgct gtacatcttc tagatcttcc agtgcatcaa tcattttttc aaatgtttct
     8941 tgatcttctg gagatagtgt tacctcgttt tgagggatca tttggatctc agcgattgaa
     9001 aaatcagtga ttcctttttc ttttaatgct tcttgtatac tatggaaatc cgctacttca
     9061 ccgtaaacga tgatttgtcc ttcttcttcc attacatcgc gaacatcgat gtctttttcc
     9121 attaggtatt caaggatctc gtcagcgtct tctcctgcaa aagcaaagat tgctgtattg
     9181 tcaaacatgt aagaaaccgc tccggaaaca cccatgttcc cgccattttt cccaaatgct
     9241 gcccgtacgt ctgctgctgt tcgattcaca ttgttcgtca atgtatcaac aatcaccatt
     9301 gagccgcttg gtccaaatcc ttcatagcgc aattcagagt atgtttcatc tcctgcacct
     9361 tttgcttttt cgattgctcg gtcaataata tgttttggta cattataagt tttagcgcga
     9421 tcaatgacga aacgtagttt ttggtttgca tgcggatcag gatcacctga ttttgctgcc
     9481 gcgtagattt caatcccaaa ttttgcataa atccggctat tattagcatc tttagcagtt
     9541 ttctttgcta cgatatttgc ccatttacgt cccatatttt cacttcgctt tctgatttga
     9601 tagtttcata acgcaaaaac gtcctgttcc attatacttg tgaatcacga ttctgtgaag
     9661 ctttttttct ttcttccttc attcttctca tttttagtca attatctata ctcggattgg
     9721 gatttgcccg aaattctgtt ccggaatatt gcctcccaat tgatagattt gatcacttct
     9781 aactaacagt tccgcttctt gtgcctcttt ttttccgatt cgggaataaa tagaaatctc
     9841 ttttttttct tttaagaatc ttcttccagc atcggtaaat ccaagtagtc gaagataatt
     9901 tttattttgt tggacaggaa tctcttgctt tttgatattc agcaagacat aacaagataa
     9961 tcgttggatc cgtgtccatg tatatctttt tgtttttacc tttgacacaa aatcctcaaa
    10021 actatcagca gttgcagctg ctgttttcat gcgcgattct attccttcgg tcatctgata
    10081 gatatctgtc agttccagca aagtagaaga taacaaccga taacgcaaat acggccaata
    10141 agattcccaa gtctgcagcg aacaagttga aagatcttct tctgtctgag gcggcacaag
    10201 tccagctatt ctttttcctt cttctaacgc atggcgaatg gctgttgcac tggctatcct
    10261 ctgatctgtg atgatttttt catgatagcc tgcactttta cgtcggatcg gatgtaattc
    10321 catagggtgg acatacttgg cattttcctt tgcataactt aatcccaaga tatgatttgg
    10381 cgaagaaaaa tcaagcgtaa tatcgggata cagactgcga aaaacctcag tcattttttg
    10441 cggataacta tacgtttgat cagacagttc ttgaaatgcc tcttctatcc tatcttgatt
    10501 ctcttggaca aagcgtccga aactgctgta atcaaacgaa gtctcttctt ctgttccaaa
    10561 acacaaactg tcacagtcca gtgtttgcaa gatttttact gcacctttag caaaatagtc
    10621 agctgactga accgcccatt cgacgggaag ctccactact aaatccacac cattttgtaa
    10681 agcttcattc gcgcgatgcc atttatcgat gatagccggt tcgcctcttt gcaaaaaatt
    10741 cccgctcatt acagcaatga cgacctctgc tcctgttatt tttcgtgcca tttccacatg
    10801 atagcgatgt ccattgtgaa aaggattgta ctctacgata atcccgcagc ttttcatgcg
    10861 catcctcctt catagttatc gataaaaaag aaatagagac aagtaaaaaa aggcaaaaat
    10921 cggaaaaaat acccgaacta tataaactat tctcgcaaac acaatacttc tgcgacgaaa
    10981 agctgcactg caagagcaat gcctatatcg ttcggatata taatttatgt tgaagattta
    11041 ttcttttctt gttaactcga gcaggcaaaa ccgctagtct ttgtatgcta caaagaacca
    11101 gcgctttgat ttctctgatg ctgcttcgtc tgtaaaatca gaaaatgctt cgactttcga
    11161 aaagccgctg ttttccagca ttcttaaata actttccaat ggataggtac gctcttgatg
    11221 tagttcatct tcacgaataa atcgaccaga cccttcttgt tcttcaacga aaaaggtaag
    11281 aaaatgttcg atgctgtgtt tctcgcttcc tggataacta tcccacaaaa aagcaaaatt
    11341 atccccttga taatgatagc tatattctgg aaaaacctca tctacttgat aggtggagtg
    11401 tacgtcaaat aaaaatcggc cattttcttc taacgcttga tagacttcat caaacacttg
    11461 ctgtacctct tgtctatttt tcatatagca cagcgaatca gaaaaacagg taattgcctg
    11521 atattgtccg atttctgata aatcgagcat attgccttcc acaaattgga tcgatgcgtc
    11581 ttcttcatat gctcgctggc tggcaatcat caacatctct tctgaaaggt ctaaccctgt
    11641 cactttatat ccatcttttg caaaggcaac cgctaatgct ccagtaccgc atgccagttc
    11701 caaaatattt tttgtgtctt cttgtaaatg acgtttcgaa aaagctaacc attgctgata
    11761 aagtgtttcg tccatcactt catcgtaaat gaaagcaaat gtttcataag ccatatgtta
    11821 atccaaccat tcactaagat cgactagcgg agcatctgac catagttttt ccagattata
    11881 gaaatttcgt tctggcgcat ggaaaacatg tacgatcaca tctccaagat cgatcaatac
    11941 ccattttccg ccttcttttc cttcaatacg ttttacttca tatttgtttt cttcttcttt
    12001 atcaatgatt tcttctgtaa ttgcgttgat ttgacgttcg ctatttgctg aacagatcat
    12061 gaaataatct gccaacaagg aaacatttcg aacgtctaaa gcgataatgt cttctgcccg
    12121 ttttgaatct gctgcttcaa tcgcaatttt taattgttct ttactatcta tgatgatttc
    12181 ctcctaatat gagcagctta tttttttgct acccagtaat tatatgtttc gattgttttt
    12241 ggataaatct gttgttcttg cgcaagcaaa tgggccaatg tatgttttgt ttcaaatgct
    12301 acagcttcgt ctagatcagc ccatgcaatg gctcgtgcat cttgtacccc aggaaaatct
    12361 cgacctggtt caatatagtc tgctacataa atgattttgt caaggagact catcttagca
    12421 gccccagtcg tatgcacccg gattgcatga agaatctctg catcggtgat tcctaattcc
    12481 cgctctacaa acgaagcgcc aacaagacca tgccagattg cattattata attcaataac
    12541 tccggatcaa aaccgtctct tacgattacc attttgaact cctcatctgg tcgttctttt
    12601 gcataatcat gtgtcaatgc agcaatacta gctttttcag gagaagcacc gtattttttg
    12661 gcaagagcca cggctgtttc ttctactcca agcacatgct tgaagcgttg ttcgctcatt
    12721 ctcatctgta ttttttgcat caggttttct ctagaataag cagtatattc attactatag
    12781 tccatattca tacaaccctt cattttgaat ataatcaatt actttatctg gaacaaggta
    12841 gcggatagaa cagccttctt tgatcttttg acgtattttc gtggaactga tgtcgatctc
    12901 aggtacatct acccaaatta caggatatgg agtatccgtt gtgtatcctg gtcgacgtat
    12961 acccacaaag ttgaccatag aagtcagctc atcgatttta taccattttg gcaaatattc
    13021 taccatatcc ccaccgataa taaagtaata atctgtatcc ggattgtttt gcgtcaattc
    13081 cttcatagta tcataggtgt aacttttccc accacgagct aattcaatcg tttcaatctg
    13141 caaaaaagga ttgtcttcca ccgctaattc caacatgttg agtcgatgtt ttgcatcaat
    13201 cgtctgtttc tcatcaacat gaggtggctg ataagaaggc atcaaaaata cgcggtcaag
    13261 acctaaattc cggccagctt gttcagccat cactaaatgt gcaaggtgaa cgggattgaa
    13321 tgttccgccc aagatcccta cttgcttttt cttctctaag aattgaggca tttcttcagg
    13381 aaataattgt gaacgaacaa atgtcttagc ttgcgtattc atcatggtca ctccttatat
    13441 tgcttttact tcttttgata gcctttgata cttttctttt gtggaaggtt tgtataatac
    13501 aataacacgt ccaatgattt ggacgatttc gcaatctatt tctttttcta atacttgagc
    13561 tgcttcttct gcaatctcat ctgtattttg aagtaacgat actttgatca gttctctttt
    13621 ttctaaagct tcttcaattt gaacgatcat tgctgaattg atcccacctt ttccgatttg
    13681 aaaaatcggc tgaagatgat gcgctttgct acgtaaaaaa cgcttttgtt ttcctcttaa
    13741 actcataatt ttccttcttc cttaaatcaa tgctttgcgt ttcaatacat ctacaccctt
    13801 tggcgcccag cccgcaatta ctactggcgc tgttacagtg atccagccta gaccggcaaa
    13861 aacgatgtct gttttttctt tcacagaaaa ctcaaaacga acaagctccg gaaatgcctc
    13921 gacttcatca ggacgaggag gctgtaataa tccacctaca tgtttttgat aaaattcgtc
    13981 tgctttttcc agtttcgttc gatgaatatt tacatcattt gacacataag caataaatga
    14041 agcacgattg ccgctgataa aatcaaatct tgcaagtccg cccaagaaaa gtgtttgccc
    14101 ttcattcagc tgataaactt taggtttgat ttcttttaac ggtgcaatga tccgcaaatc
    14161 ttttttacct aagtagtgcg ccatttgatg gcgatggata attcccggtg tatcaatcaa
    14221 aaaatgcccg tcatctaaag gaatctcgat tttatccaag gtcgttcctg gaaactgcga
    14281 agttgtgatt acatcttgta ctcccgctgt atttttgatg atttgattga taagcgtcga
    14341 ttttccgaca tttgttactc cgacgatata gacatcccgt ccatcgcggt attcttcgat
    14401 tttttctaat agttccgtca tctcatgaga tttcttagca ctagttaata atacatctac
    14461 tggacgaatt cctgcttcgt aagcacgttc cttcatccat tgtgttaatt tagttttttt
    14521 caatgattta ggtaatatat ccactttgtt cccaactaaa agtactggat tgtcaccgac
    14581 aaaacggtgt agtcctggga ttagtgaacc attgaagtca aaaatatcca ccacattgac
    14641 gatcaatgca tctttttgcc ctaattcatt cagtaagcgc aaaaagtcgt catccgtcaa
    14701 tgagacatct tggatctcat tataatggcg taatctgaaa caacgctgac aatagacttc
    14761 cccggaagct aaaccttttt ccaaagccgt ttgcggcgtg tatcctaatt caccgggttt
    14821 gtttgtttgt ataacggcac cacagccgat acattgtaat tcttctgtca ttcgattcca
    14881 cctcgccagc ccatatcagg gtgttttgct aatagatagg ccataattct tttttcccaa
    14941 aaacgattaa attgtgtctt ccaactatct gttgcgataa tgggttttac aaggatgcta
    15001 cgtatccctg cacgattggc tccacgtata tcagtcataa tctgatcgcc taccattact
    15061 atctcatcat ctgctaaatc caattgtttc tttgctatgt tgatccctct tgctaaaggt
    15121 ttcattgctc gtgcaacata caaaagatca aatttttcaa tcgctcgcgc gacacgtttt
    15181 gaattattgt ttgaaacaac aacaacaggg attcctgcat ttttcatttc taaaatccaa
    15241 gtaagcaact cttctgttcc atctggatta ttccatgcaa tcaacgtatt gtctaaatcg
    15301 gtcaatactg ctttgatgcc tagattcttt aattgggcag gtgtaatctg atagatggct
    15361 tctaccatcc atgttggttt gtaatttgaa aacatgctgt ctcctcatct gcaaaaaatg
    15421 aaggagcaca taaagtactc ctcaaccccg tattatactg catatcagca aaaaaaacta
    15481 cctctatact cgttcgtctt tcagaaaaag catttcttta caaaatgacc aaaaataaag
    15541 attgatcaca gaaaaattta gaaacaaaaa acaactaata ttggcggaac ctcttgtatc
    15601 gtttttccag taatttatgc tgatctaatc gaacaagttc cgtcaatctt tcaatcaacg
    15661 cttttttcat aatgtgtgcg actttatatc gtggcaactg ctcgccgttg aacgtttcag
    15721 gaataactcg atcaacgatt cctaactcgc gtagttctgg cgcagtaatt ttcatcaact
    15781 cagctgcttc tgcggcgcga ctaccgtctt tccacaaaat cgacgcaaat ccttctggag
    15841 aaagcacagc ataaatcgtt tcttccagca tccagacttc atccgctaaa cctagagcaa
    15901 gggcaccgcc acttccacct tcaccaatga tgatggcgat gatcggtacc tttaaatcag
    15961 acatttcgag cagatttctg gcaatagcct ctccttcgcc gcgctcttct gctcctacac
    16021 cgcaatatgc acctgctgta ttgatcaagg taatcaccgg tctattgaac ttttccgctt
    16081 gtttcatcag tcgtaaagct tttcgatagc cttcaggatg gggagaacca aaatttctgc
    16141 ggatattttc ctgcagatcc cgtccttttt gaataccgat cactgttacc ggtttgccat
    16201 ctaatcgagc gattccacca acaatcgctt cgtcatctgc aaataaacga tcaccatggc
    16261 attcctcgaa agaatcgaag atctcttcaa aaaaatccaa gcttgtccat cgttctttag
    16321 ctcttgctaa ctgaacgaca tcatgtgctg tcttatccac gatctatcca tcctttctgt
    16381 gtatgcaatt caagaagcgt atggattttc tgtttcaatt ccatgcgggg aacaatctga
    16441 tcgacaaaac catgagataa taagaattca gccttttgga aatcttctgg aagttcctgc
    16501 ttgatcgtct gttcgatgac gcgtcttcca gcaaaaccga tcaagctttg gggttctgct
    16561 agaataatat cgccttccat tgcaaaactg gcagttactc ctccggtcgt cgggtccgtt
    16621 aaaacagtta gataaaaaag accttcattg ctatgccgtt tgacagcagc agagattttt
    16681 gccatctgca ttagagaaaa gattccttct tgcatccgtg cgccacctga agctgtgaac
    16741 aagacgactg gaagactttc ctttgttgct cgttcgaata atctggtgat cttttctccg
    16801 acaatcgttc ccatacttcc catgataaaa ttaggatcca tcactccaat aatgaaggga
    16861 atcccctgaa tggttgcttt accagtgagg actgcttcat gaagtcctgt tttatcttgc
    16921 atttttttaa ttttttccgt atattcggga aatgctagcg gatctttggt tacaagatcc
    16981 gtatcccatt cttcaaaact tttttcatca ataatcaaag ctagacgctg ccatgcaccg
    17041 attcgaaaag cgtatccgca atgagggcat actttttctt caccgatgtc tttcgtataa
    17101 aggatatgtt tgcaatttgg acactttgcc cacatgttat caggaacaga tggcgagtta
    17161 gcaggtttgc tctctctatt aggattgatc cgaatatatt tctttttctt aaacagagcc
    17221 atctatgcgc ctcctatctc cgtttctggt gtccaattcg gtaaaaattc ttcttgtaaa
    17281 aatgctgtgc tgtaatcacc tgctatgact gcaggatgac taatcaaatc catctgaaat
    17341 tctgcgttcg tgatgattcc ttctgtgata aattcactta aggctcgctg catcttcatt
    17401 aatgcttcaa aacgcgtatt tccatggacg attatttttg caatcattga atcataatat
    17461 ggcgggatcg tatatcccgg gtaaactgca ctgtctactc gcagtcccat cccacctgct
    17521 ggaagcagta agttttggat cttccctgga gaaggagcaa aatggaaagc aggattttct
    17581 gcattaatcc ggcattcgat tgcgtgtcct tggaatacta cttcttcttg acacacattc
    17641 aaaggctcac cggctgctat ctccacctgt ttttttacta aatcgattcc tgtgaccatt
    17701 tctgttactg gatgttccac ttggattcga gtattcattt ccatgaaata aaaatcgcct
    17761 tcttcatcca tcagaaactc gatcgttccg gcattttcat aattgactgc tttagctgct
    17821 cgaacagcag cgtctcctag tgcttctctt tttgtttgtg agatcactac agaaggagat
    17881 tcttccagta ctttttgatt gttgcgttgt aaagaacaat ctcgctcgcc taaatgaatg
    17941 acatgaccaa atcgatcacc taagatctga acttctatat ggcgagcagg ataaatgatt
    18001 ttttccaaat acatatcatc atttccaaat gccgctgctg cttcttgctg agctgaagaa
    18061 aaatgctgtg ggagctcttc ttttgattga actttccgaa tgcctttccc gccaccacct
    18121 gcagctgctt tcagcatcac tggataacca attctgtctg ctatttccaa tgcttcttct
    18181 actgtagata atacaccagt acttcctgga atgactggga catttgcttc ttgcatcaat
    18241 tgacgcgcat tgatcttatt tcccattgca tcgatcgttg cacttttagg accaataaat
    18301 gtgatattgc actcttcaca catggaggca aatcggctat tttcagataa aaaaccaaat
    18361 cctggatgaa ttgcttcggc ttttgtcacg attgcagcgc tcagtacttc ctgaacattc
    18421 agatatgaat caacggcttt tgccgggcca atgcagattg cttcatctgc taactgtgta
    18481 tgaagtgctt cggcatctgc ttctgaatat atcgctacgg tttggatccc tagttcgcgg
    18541 catgcacgaa tgatacgtac agcgatttca ccgcggttag ctatcaatac tttagaaaac
    18601 atatttcacc tatccaatca taaaggttaa ttccgcttcg gctacttttt taccgtctac
    18661 tttagcgatt cctttgccta ttccggctga agctttcact ttcaatatct caacttcaag
    18721 gtataaggta tctcccggtg taactttctt acggaatttt gctttgtcca atccgccgaa
    18781 ataagccgtc tttcccttga attgttcaag agaaagtaat gcaactgctc ctgcttgagc
    18841 cattgcttca acaatcaata cccctggcat aacaggttct tctggaaaat gtccttgaaa
    18901 aaagggctca ttgatcgtta catttttctt agcaacgatc cgttctcctt caaccatttc
    18961 ttccactcga tcaatcaaaa gcattggata acgatgtgga atgatttctt ttatttcttg
    19021 tatgttcatt gttttccctc tttcctgctt tgcttatttt tgctgaatcc taaagagtgg
    19081 ctgtccatat tcgacaactg actcgttttc tacaaggatt tctgtgatca ctccgtcggc
    19141 ctcactagtt atctcattca tcagtttcat cgcttcaata atgcagataa cttctccttt
    19201 ggcaacttga tcacctactt ttttgaaagc tggtttgtcg ggcgctggtt gcagataaac
    19261 gattcccacg attggcgaaa caacctcgat tccatcaaat actgccgatt ctttttcttt
    19321 cattatttct ggagcagttg ttgcttgttc agatatgaca ttgactggct ccgaaactgg
    19381 caacgtttcg tttgtttgaa ccatattcat gcctcgactt gttttatttt tattcatata
    19441 caattcaaat tgaccttcac gcaaatcgaa ttccgttagc gaggattgat caaattgact
    19501 gactaattct ctgatttcat tgatatccaa aattattcct cccagcgttt catgcacaga
    19561 accgcattat gtccgccaaa ccctaatgaa ttactcattg cataagtaaa acttgcaggt
    19621 ttgctttcgt ttttgacaac tgtcaagtca atctcaggat cttgtttttc tacattgatc
    19681 gtaggtggga taaattgatg ttctaatgcc agcaaagttg ccaaggcttc gatcccgccg
    19741 gctgctccca ataaatgtcc agtcatcgat ttcgtgctgc tgacgtaagt atctctgaaa
    19801 ttttcaccta atgcgatttg gattgctttt gcttctgcct ggtcattggc tggtgtactt
    19861 gttccgtgag cattgatata tccgacttgt tccggctgta caccagcttc ttccatcgca
    19921 agcttcattg ctttagatgc accagaacca tctggagtag gagctgtcat atggtaagcg
    19981 tcacagtttg caccgtatcc aacgatttct ccatagattt tggcaccgcg agcttgtgca
    20041 tgttctaaag attcaagaac tagaacgcct gctccttcac ccatcacaaa accattccga
    20101 ttttcgtcaa acggaatcga accacgatta ggatcagttg attctgagag tgctgttaaa
    20161 gctgcaaatc cagcaatccc catctcacaa atcgttgctt ctgttcctcc agcaagaatg
    20221 acatcagaat agccatgttt gatgttacgg aatgcttccc caattgaatt cgttccgcta
    20281 gcacaagctg ttacgatagc tgtacaaata ccttttgcac ctacacgcaa agcaatattt
    20341 ccagctgcca tgttggcaat cgccattggt acgaacattg gagaaactcg ttttgcacct
    20401 ttttcatgta gacgaataac ttggttttcg attgttggaa gaccaccgat cccagatccg
    20461 atgatgacgc caaaccggtc ttgattcatt ttttctttat ctaaaccgct catctccatt
    20521 gcttctaaag cagcgtagat tccatagatc gaaaacatat ccattcgttt gctatctttt
    20581 ttaataaaat atttatcgta agggaaatct ttgacttcac ccgctaaggt aatccctgtt
    20641 tctgaagcat caaatttagt gatcggtgcg atgccattcg tcccctcttt taagctattc
    20701 aaaaagctgt ctgcgtcatt tccaataggt gaagtgactc catagccagt aattaccact
    20761 cgattcattt gtttgctctc cttttcttat ccgtgcatga ccaatccgcc atcgacattg
    20821 atgacttgtc cggtgatata tggattttgt gctaagaaaa ctgctgtttg tgcaatatct
    20881 tctacttgtc cgaaacgctg catcggaatt tgcttttccg cttgttcctt gactttgtct
    20941 gctaaaactt ccgtcatatc agttgtgatg aatcctggcg caatggcatt acatgtaatg
    21001 cctctagttg cagcttctcg tgccacagat tttgttaatc ctactacgcc agctttacta
    21061 gctgcataat ttgcttgacc aatatttcca atcaacccag atacactaga gagattaatg
    21121 atggcccctt ctcgctgctt catcattttt ttcaatacgt gctgcgtcat attgaaggtt
    21181 ccaatcaaat tgatatccag acattttttg aaatcttctg catccattcg catcacaagt
    21241 ttgtcatttg tgattccggc attgtttact aacacatgga tcgatccaag cttttcttct
    21301 gcttctttga tcatttgtcc cgctttctca taatcagaga tgtcaccgga aactcctacg
    21361 cattttacac cgaatgcttc gatttcttct atcttttctt ttggaatctc tccgcgacca
    21421 ttcaaaatga tatttgctcc agcttttgca aaagctaagg ccatcgcttt gccgattcct
    21481 cttgtactac ctgtgataaa tacatttttt ccttttacat ccataacgtt cctccttata
    21541 gtattgctat tgtttcatca aatgtctgct tattctcaac acgcagaact ggtattgttt
    21601 tatcgatttt tttcataaat ccgcttaata cttttccggg gccgacttcg atgacttgtt
    21661 cgatgccgat tgtttttaac ttatggatac tctcatagaa acgaacaggt tgcatgactt
    21721 gctgttcaag tagtcccgcg attgtttctt tcttcattat ttccgttgtt gtgttgctta
    21781 tgataggaaa agaaggttca gagaattgaa tctgacttag gtccttcgcc agtttttttg
    21841 ctgccggttc taaaatagct gtatgaaaag gcccgctgac atttagaggg atcattcttt
    21901 tgaacccttt ttcttttaat agtaaaacag cttcgtctac tgctttttct tcgccaccaa
    21961 tcacgatctg ctgaggtgta ttataatttg caggagatac gatgccgtac ttgctagctt
    22021 catgacagct ttcctcgatc gtttcaatcg gcgcattcat cactgcaacc attttcccgc
    22081 ttccagctgg ggtagcttct gtcatataag cgcctctttt tgctactaac gcaactgctt
    22141 cagtgaagga tagcgcatta ctagctacta atgccgaata ttctcctaaa ctcagtccta
    22201 aagctgcatc tgggatgatt ccatgctcct tcaataagcg ataatacgcg atgctgactg
    22261 ttagaatagc tggctgtgta tattgcgtct gatccaaacg ttcattttct gtaaagcaaa
    22321 gttcagccat atcgtagccg agaatctcac ttgcttcatc aaatgtctgc tttacaacgg
    22381 cttcttcata taaatctttt cccatacctt gatattgtgc accttggcca ctaaataaga
    22441 atgctgtttt catgtttttc tccaatatat ccagaatata tgtttgattc ttcctattca
    22501 aaaaggccga gacattccta tttcaggaaa tgtcccagcc cccaacctaa ttttaatgat
    22561 tatgcagttt gtttttctac gtaagtcact aaatcttgaa ccgttttgat tccgtcttct
    22621 gtttcgattt tcacgtcaaa ttcatcttca atttcattga tgatttggaa taaatccaag
    22681 ctgtcagctt ctaagtcttc ttggatgtca gtagtcaatt tgatttcttc agtttctttt
    22741 cctaattctt ctgcgatgat ttcttgaatt ttttcgaata ccataataat atattcctcc
    22801 gatattgttt gctttttatt tttataattt aacagtctac aactccatga gaatacttcc
    22861 ccatgttaaa cctccgccat agcctgtaaa tattactttc tgctgttttc ctaaggtgat
    22921 ctttccacta gaaactgctt catccaataa gatagggatg cttgcagcag atgtattccc
    22981 atatttatcc atattggtca ggaacttttc ttgcggcatc tttacttttc gggcaatttt
    23041 ctcaataatt ctgaggtttg cctgatgcaa aagcagataa tcaactggtt tgtctttcat
    23101 gacttcttgg atattgtttg caacgtctcg tacggcaaaa tcaaaaatat ctctgccagt
    23161 catttgcaaa taatacgaac cttctgaatc ttctgaatag aacggatttt tattttgctg
    23221 gtagccagcg gtcaatgagc tacttctggt accgtcagct gctaattttt ctttcagcaa
    23281 ttttttctgt ggattcgctt ctaagagaat cccacctgct ccatcgccaa acaacacagc
    23341 agtcgtacga tcagaccaat cgattagctt agatagtgtt tcaccaccaa tcaccagtcc
    23401 cttagttgag ccgcagcgga tcattttttc agccatgctt aacgcataaa caaatcctga
    23461 gcacgcagca ctgatatcaa aagcgatagc atgagttgct cccaatttcc cttgtacttg
    23521 tgctgctaca gatggtgtcg tgtaatcggg cgacattgtt gctactaaaa taaaatccaa
    23581 atctttaggg tttagatttt tctgttttat caatttttcc gccactaaaa tgcataaatc
    23641 agaagtctcc tgatttgtta cacatcttcg ttcacgaata cccgtccttg acgaaatcca
    23701 ttcatcagag gtatccatca tcttggaaag atcattatta gtaataatct tttcaggaag
    23761 gacgctggct gttgcagtaa tcattccaaa ttgttccatt aaatatcctc acttatagtc
    23821 ttgcaagaag tcgtgcaagt ttttcagtgc atccagcaag gcatgctgtt cctccgtagc
    23881 cattccgtct aagatgttct tgaccatttc acgatgaaaa tgctgatgga cccgaaatag
    23941 taactttcct tttttcgtta gtcccaactt tacaacgcgg cggtcatctt cactgcggat
    24001 acgttcgaca tagccttttt ttactaactt gttaatagca gtggtcaagg tcccaacagt
    24061 aattgatagt tctttggcta cttctgaagt tgtttttttc ttgtacatcc cgattgcctc
    24121 gatcgtgtgc atctctgtga ttgacagatc tttgaattga gattttttca gctcagactc
    24181 ttcgattgtc aaaatatcat tgaagacact aacaagataa tcatttactg tttttaaatc
    24241 tgattccatg ttccaccgtc cactaattag tttgattatc aaatcttttg atgttcaaac
    24301 tatatcttaa cttttttcaa aatgcaacct taaaagatca tttttttact atatgagctt
    24361 tttttaagat tttactcatt taaaacaact gataaatcag catttataat ttcttttatt
    24421 agaaaaatca tctatctcac atattttgat tatcaaagta ttttctgtga acagaactat
    24481 agaattccct aaaacttgtc cattatttca aaatttgtta tagtcttttt attcaaagcg
    24541 taaaaggaga tcctatgtta aaagaaaaaa tgaaaactct ccccatgctg ccgggtgtct
    24601 atcttatgaa agaccgagat ggtaaggtca tctatgttgg taaagcaaaa cggctcaaaa
    24661 atagagtaag cagctatttt catcagaata aacagcattc aaaaaaagta ttacgtatga
    24721 ttcatcatat aacagatttt gatttcgtcg tagtagacac ggaattagat gctttattgc
    24781 tcgagtgtca attgatccaa cattaccgtc ctttttataa ccgacaaatg aattatttca
    24841 gtaattataa ttatgtacat attaccaata agggatttgt attaacagat actcctacgg
    24901 ctcgaacata cgggcctttt cgtctctata aaaaaatgcc gtctattttg cgtataatgg
    24961 aagaaactta tcaaatgcct tggttgtctg aaattagttt gttagctttg cgagtccagc
    25021 ttcctgacct acaggaaatg acttttgaac agaaaaaaaa ggaactccaa gggctgtttc
    25081 aaggccgaaa taaaaagcta ctgacttact tgaaaaaaag acaacaacat ttcatctatc
    25141 agttgaattt cgaaaaagcc ggtatgcttc aaaaagatat cgaattagtt acttatttta
    25201 ttagacggat ccaggaacaa aagcagtttt taagaacacc atcgctcacc ttcagcatgc
    25261 cattagcagc agatgagagt cagaaaaagc actacctcgt ttgttatggt caacttgcag
    25321 aaacaatgat tacctctggt gacgtttctc ctgacttcta ttatgaaaag aaagaagctc
    25381 atttatcttt gaaacgtcaa ttgtcaaaag aagaaatcga tcctgtacaa atcttgatca
    25441 gttaccgcaa aaaattagaa aaagaacagt ttgaaattga actgttaaac aaaaaagaag
    25501 ctgaaaagca actaaattag ttgcttctcg gcttctttta ggcatcttca acttcaaatg
    25561 tcaattatcc taaaatttat tggattaagt catagatttc catcgctacg atatcgatat
    25621 tatcaaattc gtattgctga tgagtatctg tttcagttaa ttcaaatgta tcggtcgctt
    25681 tatcataagt tactgtacac ttctctacgc cttctttttc aaaacggcga acttgcagtt
    25741 cattatctgc tgactcgacc atagcctcca gtcgttttat gattgcgact aattgtgaat
    25801 gtgtcatcgg aggtcctcct tttcttattt ctgttatatt gtatcaaaag catagcctac
    25861 ttgcattgat tttctctaaa aaaactcgtt ttttatgatt ttttttctgg tatttttcct
    25921 tcaggccacc aaagtttgat caaagcttgg gttccgtcat tctcaaaacc tttatctgcc
    25981 aactcttcat atagtttcaa tgcttgggta gttgctggca atacaagatc cattttttct
    26041 gcttcttcta atgcaatctt taaatctttg ataaaatgtt tgacaaaaaa accaggtgtg
    26101 taatcttctt ttaaaatacg aggagaataa ttgcttaaag accagttagc tgcacttcca
    26161 cctgaaagcg tctcaatcac tttctttaaa tcaagtccgt ttttttgtgc atagacaagc
    26221 atttccgtca ttcctgtcat cgtacctgca atcatgatct gattagccat ttttgtatgc
    26281 tgacctttcc cagcagaacc atgcaatgta aatgtcgtac caaattcttt gaataaaggc
    26341 agtacttgat cataaacggc ttcttctcca ccgaccatga tcgtcagtgt accattcttt
    26401 gctcctaagt caccaccgga taccggtgca tctaacgcat ctgcgccttt ttcttttgct
    26461 gtttttgcaa ttttttctgc caggcttggt gtacttgtag tcaaatcaac taaaatatgt
    26521 cctgagatat ctgcagaaaa aatccccgac tgtccatagt agatttcttc tacatctttg
    26581 gggtaaccta ccattgtaaa gagaatatta ctttgttctg ctaccgcttt tggtgtatct
    26641 gcccagatag ctccttctgt tactaaagga tcagcttttg cttttgtccg attatagaca
    26701 gctacttcgt gtcctgcttc tagtagatgg cgtgcgacag cacttcccat gacgcctgtt
    26761 ccgataaaac caagtttcat tttttcgacc tcctaaaaat caaaaatcct tgcttctatg
    26821 ctagtatacg ctagaaacaa ggattttttc ataaaaaatg tttgaatttt atcttttaaa
    26881 tgaatttaat tttattttga ttcgttcaat cgagtcttct tcttcttttc ttcgcatctc
    26941 ttcttcatga taaacaacca tgtcttgata aaattcgatc atagcttcgc cgaaatgtgt
    27001 agatgaaata tcataaagtt tagcttctaa aacttttgga tctttaggaa cttcactgga
    27061 aatataatca ataagtgctg gtgcaaagtc ttcgtcggtt tcaaaagttt tccctaatga
    27121 aggatgatcg aacaatcggt tcaaataagc atttccttcc acgacacaag gtgttccggc
    27181 tgccatcgct tctgtataag taagcccttg tgtttctgaa gtagaagcac tgacaaaata
    27241 atctgccgct ttataataaa gtgctacatc attgttgtcc acttctcctg tgaactgcac
    27301 aaattcttct aactgcagtt ctgagatcaa ttcctctaac tcttctacat aaggtccttt
    27361 tcctacgata actaatctgg cgttaggata agcggagatg acctgaggaa acccattaat
    27421 caaggcttgg atatttttct cataagaaat cctgcttaaa gaaagaatca tcaaatggtt
    27481 ttcctgaaga cctagtgact cccttaattt gctgatatcc tgctgagtga tatcagaacg
    27541 tttgaatttt tcaatgtcga taccagtagg aatcacgcga agcggtgcta ttacaccata
    27601 gctgcgtaat gtatcaataa cacgttcact tggacaaact acacctgtcg tatgatttgc
    27661 aaataatcta gacaagtatt ttacatggga ttgcctgatc acttttcctt taccgatata
    27721 atgaaggtag tcttcataca tcgtatggta agtatggatc agaggtattt tcaacttctt
    27781 agctaccatc ttcccaagta tccctgctcc gaattctgta tgggtatgaa taagatctag
    27841 ttctagttct ttggcaacca aataagcata ccacatgcct ctaacaacaa ctcttcgatc
    27901 cttgaaagaa ataaatggta cacttggcat tcgaatcaca tctttttcca acttatccgc
    27961 attaggatcg gtcgttgtaa aaatatagac ttcatgccca tgtctttcta attcttgttt
    28021 taatgttttg atcgatgtag caactccgct gacttgcgga aagtaggtat cggtaaaaaa
    28081 gccgattttc atgtttttcg cctccaaact agtccttgac ctgatttact ttttttaatt
    28141 tgctagagac tttttcattc agtacacttt cgtatacatt tttaagttct tcaccgattt
    28201 tattgatact tcgctctttt gctgtttgat aaccggcttg tgtcgtactc ggtagttctt
    28261 gattggtaat ctgttggatc agtcggataa attcttcgtt tgtcttcccc atatagcagt
    28321 tcttgctatc ttctaaccag ccattataca ctggaatatc tcgaacaagt acgttttgtt
    28381 tgcttgctaa agcttctaaa acaacgatcc cttctgtctc ttcataagaa gggaaaaaga
    28441 atagatctgc acctgaatac gcaccttcta tgatctctcc acgaatgtaa ccaggaaaca
    28501 ggacattttc aggatgatct tcttttacaa ttttccggat gttccgagga atcgagtaca
    28561 tgggaacatg accaaaccaa ataaatgtgt attcgggcat cttcttagca atttctacga
    28621 aatcaatgat tcctttacgt tcgaaaaaca aaccgacaca aatgattact ttttgatccg
    28681 gagacagttt gaaatattct ctgaactttt gttctttcat tggatcaggg tgatatttct
    28741 ctaagtcgat cccattggaa atagcttgaa tcggcacacg gataccatag ctttccaaca
    28801 actgttttga ataaggtgtt ggcgtgatca gatagtccgc tttttggtac aatcctacca
    28861 gatatttttt tacgatagga gaaagctggt tcgatccgat aaaggaattt cggaaatctt
    28921 cctctgtaga atgcgcatga tagacaactt tttttccttc tcttctggct ttattgacca
    28981 tattatgact attgattccg tatgtattga tgtgcaggat gtcataatct tctttttcat
    29041 ctagtgtata agagataccg acttccgtca gagcacgttt ttgatgatca agcgcacgcc
    29101 cgattccaga ttttgcaagc atcttttcac tttcaaaata taataatacc ttcaccttac
    29161 gtcccccttc acttatatag tataacatag cctacaaact gtcactcaaa cttaagccct
    29221 atttttgtct attcttactg acaaaaacgt tttcttaaaa aagaacaaaa aaactggcgt
    29281 agctctttta caagccacac cagcttttga tacgttgttt attttacata ttcatgaaca
    29341 agttcaacga cttcttccat catatcgcat tctttcaatg cacgatcagc aagttcagcc
    29401 attttcgctg tgtccaagcg tttcattaaa ctacgcgttt taagaataga tgtcgcactc
    29461 attgagaact catctagacc catacctact agcaatggaa cagctgtttg atctccagcc
    29521 atttctccac acatacctgc ccattttcct tcagcgtgtg ctgcatcaat tacatttttg
    29581 atcaaacgta aaattgatgg gttatatggt tggtacaagt aagaaacgcg ttcgttcata
    29641 cggtctgctg ccattgtgta ttgaatcaaa tcgttcgtac ctacagagaa gaaatctact
    29701 tcttttgcaa acttgtctgc taagactgct gctgctggaa tctcgatcat gatgccgact
    29761 tggatcgtat cagatacttc cttgccttca cttatcaatt tttgtttttc ttcttcaaag
    29821 atcgcttttg ctgcgcggaa ttctttgagt gtagctacca tagggaacat gatacgtaaa
    29881 ttaccatgaa cagatgcacg aagcaatgca cgcatttgtg tacggaacat ttcgtcgcct
    29941 tgttcagata aactgatacg caatgcacga tatcctaaga atggattcat ttcatgaggt
    30001 aattgcagat atggtagttc tttatctcca ccgatgtcca ttgtacgaac aactacaggt
    30061 tttccttcca taccttctaa tacagctttg taagcttcat attggtcatc ttctgttggg
    30121 aagtctggag aatccatata caggaattca gtacggtaca gaccaactgc ttctccacca
    30181 ttattatgga caccaactag atcttttggt gttccgatgt ttgcagctaa ttcaaaatgc
    30241 ttgccgtcag ctgtgacagt ttctgcattt tttaattttt cccattctgc tttttgtgct
    30301 gcgtaatctg cacctgcttt ttcaaattca gctttttgtt catctgtagg atcgatgatt
    30361 acatcgcctt cgatcccgtt gacagccaaa atatcgcctt ctttgacttt agcagtaatt
    30421 tcttttgtcc caacgattgc tggaatttct aaagaacgtg ccatgatagc agaatgtgaa
    30481 gtacgtccac caatgtctgt tacaaacgct tttacaaaat tgcgatctaa ttgtgctgta
    30541 tcacttggag tcaagtcatg tgcaacaacg accacttctt cgttgatcat tgatggattt
    30601 ggtaaagtaa cgcctaataa gtgcgctaaa atacgtttag caacgtcacg aatatctgca
    30661 gcacgctctt gcatataagc attatcttcc attgcttcga acataccgat atacatatcc
    30721 gttacttctt ttagcgctga ttcagcattt acgctgttgt ctttaatatt ttgcttgatt
    30781 tgacctacca tttctggatc tgaaagaacc atcaaatggg catcaaatac ttgcgcttct
    30841 gcttcaccta agctttgcgc tgctttttca cgaatttgct gcaattcttc agtagattta
    30901 gctaatgcag catccaaacg agcttcttca gccgcagtat cttctactgt tgccttactg
    30961 aatgttaaat ccggttgaac tagcaggtaa gcttttgcaa cggctacacc gtcactagcg
    31021 gcaatccctt ttagcatttc aaccattatt cagataagcc ttctttcttc attgtttcaa
    31081 cgattgctgc cattgcatca gcttcgtcag caccttctgc agtaatagta acatcagaac
    31141 cttgacctac acctaaagac ataacgccca tgattgattt caagtttact gatttacctt
    31201 tatactctaa gtttacatca gagttaaatt tgcttgctgt ttgtactaat agtgtagctg
    31261 gacgagcgtg gatcccagtt tctgctacta cgtgaaattc tttcttttcc atattaatcg
    31321 gtctccttta agtgagtgtt gtttttatgt gagggttttc ttttttagta attcagagaa
    31381 tcctaacaaa tgccaccctt tcatctgtaa agattaacat tttttttcgt tagatacaac
    31441 agttttcatt caatttcaga taaaagtatt caaattattc cggattcttt ttcatccttt
    31501 tcttcacatt ttataacaaa cgtgtttatt tcctttttct tgtcgctttc tcttctcctg
    31561 acgtttatca ttctttcctt tgaccctctt tgttttcata tgaaacgaat tacttgcttc
    31621 cctttttatt tttagtataa taagagtcaa aggtcaggaa tggtcaatag agatttcacc
    31681 ttttctacct cataaatctc ttaaaaaact aggggttatt cttttacctc atgaaaaacg
    31741 agatgtaaaa tatggatgta cccatttttg tgaaaggacg tgaatgattc atgctttgtc
    31801 aaaactgcgg caagaatgag gcaacgattc acttgtatgc cactgtcaat ggtcagcgga
    31861 cacaactcga ttactgtcag agctgttatc aaaaaatcaa aaatcaacaa aatggaggtc
    31921 tgactaatat ggcccaaaat gatccatttg gatttggcag tttggacgat cttttccgtt
    31981 ccatgtctcg ccaaatgcaa cagcaaggaa attatgagca aacaccacca acacaatttg
    32041 gcggaaacaa caacttcaat ggtggtcagc cacctcaagg caatacagaa ggtttattgg
    32101 gagaatacgg aatcaatatc acagaacaag cacgacaagg tgatatcgat cctgtgatcg
    32161 gacgcgatga cgaaatcaaa cgagtcattg aaattttaaa tcgccgaacc aagaacaatc
    32221 ctgtcttgat tggagaacca ggtgttggta aaacagcagt tgttgaaggt ttagcacaaa
    32281 aaatcgtaga tggtgatgta ccgcaaaaat taatggacaa agaagtcatt cgtttagatg
    32341 tcgtatcttt agtccaaggt actggtatac gaggacaatt tgaagaacgt atgcaaaaat
    32401 tgattgaaga gatccgtcaa gcagaaaatg tgatcctatt catagatgaa gttcacgaaa
    32461 tcgtcggtgc tggttctgct ggtgatggaa atatggatgc tggtaatatt ttaaaaccag
    32521 ctttagctcg aggagaattg caaatggttg gtgctactac cttaaatgag taccgcatca
    32581 tcgaaaaaga cgctgcttta gaacgtcgta tgcaacctgt acgggtagat gaaccaactg
    32641 tcgaagaaac gatcagtatt ttgaaaggtt tgcaaaaacg ttatgaagac tatcatcatg
    32701 taaaatatac tgatgaggcc atcgaagcag ccgcaacttt atccaatcgt tacgtccaag
    32761 atcgcttctt gccggataaa gccatcgatt tgctagatga aacaggttct aagaaaaact
    32821 tgacgatcca aatcgttgat cctaaaacaa tcgagaaaaa actgcaagaa gcagaagaac
    32881 aaaaagtttt agcttctcga gaagaagatt tcgaaaaagc agcttattat cgcgatcaga
    32941 tcaataaact tcaaaaaatg aaagaacgtc agctcactga agaggaaaca cctgtgatta
    33001 ccgaaaaaga tatggaaaaa atcgttgaac aacggacagg tattccagtg ggtgaattaa
    33061 aagaaaaaga acagacacaa ctgaaaaact tagcagatga cttgaaagct catgtcatcg
    33121 gacaagataa cgctgtagat cgagtagcga aagcgattcg ccgaaaccgt gttggattaa
    33181 acaaacagaa tcgtccaatc ggctccttct tatttgttgg accaactggt gtaggtaaaa
    33241 cagagcttgc gaaacaactt gcctacgaac tattcggttc acaagattca atgattcgtt
    33301 tcgatatgtc tgaatatatg gaaaaacata gtgtctctaa attgattggt tctcctccag
    33361 gttatgtcgg atacgaagaa gctggtcaat taacagaaaa agtacggaga aatccttaca
    33421 gtcttgtctt gttagatgaa gtagaaaaag ctcatccaga tgttttgcat atgttcttgc
    33481 aaatcttaga tgatggtcga ttgacagatg cacaaggaag aacggtcagc tttaaagata
    33541 cgatcatcat tatgacaagt aatgccggaa ctggtaaagt agaagcaaac gttggttttg
    33601 gtgcggcacg cgaaggtgtt actcgttctg tattgaacca attaaacaac tactttacac
    33661 cagaatttct gaaccgtttt gacgggatca tcgaattcag tgcattatca aaagaaaact
    33721 tgatgacaat cgtcacacta atgctagacg atgtcaacca aatgctggca gcacagcaac
    33781 ttcacatcga agttcctacg aatgtcaaag aaaaattagt tgacttaggc tatgatccat
    33841 ctatgggtgc tcgtcctctt cgacgcacga tccaagaaca aatcgaagat ggtatcgctg
    33901 aattttatct tgatcaccct tctatccatg agttgaaagc aaaactggat aaagatggaa
    33961 aaatcatcgt tacttctaaa ccagaacgtt tggctaaaga atcagccgaa gaaacggctg
    34021 aataaatcca gttttatagc gtgagacggt atttctttga gaaatgccgt ctcattcttt
    34081 tttcagaaaa gtcaaaatac tgttcatttt ataaaaaaga aattataata agagaaacaa
    34141 gcaattaaaa atacataaaa gaggtgagcg ccatgaaatt ggtaaatgta acaaacagcc
    34201 attcccgtct tgtattaaac cagcttgaaa acactgatgc ccatctagtc aaggtctaca
    34261 cagccggaaa taccactatt gtctacactg aagcccctga acataatgaa attctgctga
    34321 tcaatgacaa acgtaaaatc cagccgaaag aaatcgaaga tgctaaaaat tatttcttag
    34381 ataaaatcaa agacgccgtt taccacgagg atgaaatcaa agtaatcgaa ttggcaggcc
    34441 ttgttgaaat ctcgattcca aaacatgaat taggcgctac attagccgtg taaacaaatt
    34501 ttgaacataa taattaaaga gcgtgggaca aaagtaaaga acacttttgt ctcacgctct
    34561 aaacacgtat aaacggcgaa agcagaagca actccttcgg aaataagctg aaattcacga
    34621 aaattgcttt tcaaattttt gtgaattcca gcttatttct tggagttaaa cgcttctgtc
    34681 tcagcctctt tttctttcta gctttttgat ttttctcttt tcttttgtac ttttctcttt
    34741 tcattttcag ttctacgcgt gtctttattt ttaacttttt cctctttatg gatgattgat
    34801 cgtacttttt gtgcaatttg ctcgtttttg tcccagtctt ttacatctaa taatcgacat
    34861 aatcgtaaca tctcttcttt tttctgtttt tttctttcac gtctttgcca ttctgtttcc
    34921 atgtgtcaca ctctcctttc tttaatcata tgtacgcaaa ataattttta tatttttttg
    34981 attagacatt tctatgattt taatatagta aaaagagccg tcacggaaaa tccgtcacgg
    35041 ctctaaacaa aatatgagag gctgtttttg tttggctatt cttattactt gatgcagggc
    35101 agcttgtgtc aagacttctt tacatcaaac tttttaattt tacattggga tatttatccg
    35161 caaaccagcg ttctgcaaat tggttctcga ataagaacaa cggttggtcg aaacggtcac
    35221 gagccaagat attgcgactt gaactcatct tctcatccaa atcttctgga tcgatccagc
    35281 gagcgatttt gtgtcccatt ggagacatga ttacttcggc gttatactca tttaacatac
    35341 gatattggaa tacttcgaat tgtagctgac caacagctcc aatgatatat tcttccgtta
    35401 gatatgtttt ataaagctga atcgctcctt cttgaactaa ttgatagatt cctttgtgga
    35461 aggatttttg tttcatgaca ttttttgcag tcactttcat aaacagctct ggagtaaatg
    35521 aagggagttc ttcataagca acttttagtt ttccttcgaa caatgtatcc ccaatctgat
    35581 aattgcctgt atcatataca ccgatgatat cgcctgctac tgctttttcg acgttttctc
    35641 tcgaatctgc cataaattgt gtgacattgc ttaatttcag ttttttattt gtacgttcca
    35701 accagacatc cattcctcgt tcgaatgttc cagaacagat tcggacaaaa gcgattcggt
    35761 cacgatgggc tggattcata tttgcttgga tcttgaatac gaaacctgaa aattcttcct
    35821 cataaggact taccatctga tcatcttgtg ttttgtgtcc ataaggagct ggtgccaatt
    35881 caacgaaagt ctctaaaaaa gtctgtacac cgaagttagt caatgcagaa ccaaaaaata
    35941 ctggtgtctg atcgccacga gcaatttttt cttgattaaa tgcatctcca gcctctttga
    36001 ttagttctac ttcttctaag acttgttcat ataaggaatt ttcagttaga ggatgatttt
    36061 cgggaagcag tccatcttca tccaattcta ctaatcgttc atcctgataa ttttccggac
    36121 gataaaactc tacacgatta tggtagatat cataaagacc ttctagccct ttacccatac
    36181 cgattggcca attcatcgga taagattcga tatctaatag ctcttctaat tcttctaaca
    36241 attccaatgg ttcacgacca tcacgatcta gtttgttgat aaaagtaaat atcgggatgc
    36301 cacgtttttt tacaacttgg aataatttct ttgtctgtgc ttcgatccct tttgcactat
    36361 cgattaccat gaccgcacta tccacggcca tcaaagtacg ataagtatct tcagaaaaat
    36421 cttcatgtcc tggtgtatcc aagatattta ctcgtttccc ttgataatca aactgcataa
    36481 cagaactagt aacagaaatc ccacgttgtt tctcgatttc catccagtct gatttggcaa
    36541 aattacctgt ttttttgcct tttacggtcc cggcttgacg gatagcccca ccaaacaata
    36601 gtaattgttc tgtaatcgtt gttttcccag catccggatg ggaaatgatc gcaaaagttc
    36661 tgcgattgtc cacttgttgc tttaattttg gattattcat aacctaactc catttcttcc
    36721 attcaattat ttatattttc tctaggcaca gattgtccga tctcttaaag aaagacaatt
    36781 tcatataggg ctgtgacaaa agccgtgtag cagtctcttt ttccgagtaa acggcgttct
    36841 agagacagcc tatcagcaat aagcccaccc aagccaaaaa tacgaaaagc tgtttttgct
    36901 tggctgctct tattacttaa tgcaggatag ctttttcaca acctttgtca actactacaa
    36961 ctgaatcagt atagcatgga ttcagcgaat gaaaaagcca ccaaaccata agagtcaaat
    37021 atttttttgt cagctgacta aaagaaaaga cgtgttgctt gtttattcaa gcaacacgtc
    37081 tttcataacc aagtttccaa ttatcttctt ggctcgtcat cttcgaattc ttttctgaag
    37141 tatcttcgtt cttcttcagg ttccgcttct gtttcttccg gatcatgaaa aatgacacgg
    37201 attttaagga ttcgagaacc ttccatttct tcagaaacca gcgtaatatt atcgacatca
    37261 aacgataact tttcgccttc atccggaatc atgcctaatg ctgtaatcaa ataaccagcc
    37321 atcgtatcta catcactcat atgcagatca ctgtcaaaag cttcattgaa ctcatcaatc
    37381 agcatacgtc cttggatgat atattcgtgt tcatcgattt tttcataaag attctcaact
    37441 tcatctgatt catcatcgat ttctccgacg atttcttcta aaaggtcttc tagtgttgct
    37501 aacccaacga cgccgccata ttcatctaat aaaatagcca tctgattctg cgtctttttc
    37561 atttcatata acagatcatc aataaagacc gtttcaggaa cgaataaagg ctcctgcatg
    37621 attttcttga tatctaaatt atcaaagccg aatttatggg cagcttttag taaattcttt
    37681 gtatgaagga ttccgactac tttatctttg tcttcgttat aaacagggat tcttgaatag
    37741 ttttcggata aaacttcatt cacattttct tccacagtgt cgttgatatc aatcataaat
    37801 gcatccgttc gtgggaccat gacttctcgg gcaactttcg tatcaagaga aaaaacccct
    37861 tggagcattt ctaattcttc attattcaat acgccttcgt tttcaagcat ataacgcatt
    37921 tcatctctgg tcatttttga atcttcatca tcaaaagtca tcggcgtgat acgcgccaat
    37981 aaacttgtgg aagcagaaag cagccagacg aagggcttag ctactacacc aatcaagcgt
    38041 atcagccctg aagtgaactt tgcgacttct tctgatttat ttaaagcaat acgtttaggg
    38101 tacaattctc caaaaacaat cgatacatag gtcaaaatag ccagcacaat gatatttgca
    38161 atattttttg ccgctgtgcc gccacctaat accggagcta accgttccga taaagtatta
    38221 gctagtgaag caccagacaa aatgttgacc aaagtgattc ctacttggat tgtcgatagg
    38281 aaattatttg gattctgcaa tatttttaaa agcttttgag atttcacatc tccttcatca
    38341 gctttttgct cgattcgatt cttattgatc gaaacaacag caatttcgga agctgctaaa
    38401 aaagcattga tcaatgtcaa aacgatcaat aataaaatct gcgctaacag cgactgactc
    38461 tcagggtcag cattcataat cgcattctct ccttataaaa ttaattttta gtttacgata
    38521 gaaaaaaacg tggcactata gccacgttaa aatggtatca caaagcaatt ttctttgcaa
    38581 taaaaacaag tgtactaaaa ctacctacga ggtcgtagca attttcttct tgttttcatt
    38641 ttgcttgtct tctttgacga ttttgattat ataggaaatg atcgtcttgc agattgcgta
    38701 aaaaggaaca cctaagaaga taccaagcaa tccggcaata tttccagcta ctagaagaac
    38761 gataatgatc gttaatggat ggattttcag tgatttccca attacattag gataaatgat
    38821 attcccatcc aactgttgga cgattagcac aaccacacaa caaagtaatg cttttaccgg
    38881 atcattgaag actgttacta gaaatgcagg tgctagtcct agataaggtc ctaaatatgg
    38941 aattaggttt gtcaatcccg caatgacccc aaataagaaa gcataatcca caccaattac
    39001 catatatcct aaaaatgtga aggtaccaac gaataaacat tcgattgctt gtccgctaat
    39061 gtaattagcc aatgttttat tcagttgccc caataactcc acaatttggt ttcttctttt
    39121 ttctgggaaa aactgttgga tgtttggcac tagtttttct ccgtctttca acatatagaa
    39181 caagatgaat ggtgctgtaa tgattactac ggttgccgaa gcgatcgttc caacgatcga
    39241 acctaaacta ttggatactc cactaaggaa ttgctgaatg attgtcccgt aagaaatgtc
    39301 taattgctca aaatatttat tgatatctac ttccttgaac aaaggcaatt cggctacctc
    39361 ttttaaccag ctttctacct gtttgatgaa atcaggcata ttagatgcca atgaggaaat
    39421 ttgctgtact agacttggga tgacactaag taaaatccaa ataattgctg caactaaaag
    39481 gagcagcaca attgcgacag cccatattct tttgattttt gctttcatca acagcttgac
    39541 aataggattc aataaatagt aaagaaagcc tgcaatcagt acaggtgcaa ataacgtgga
    39601 aaaaaatgtc ccgatcggtt cgaaaataaa gtttatttct gatgaaatca tgatcaatgc
    39661 agctatgatc aataattcaa gcgaccaaaa cattagttta gactgtctaa gtttttcaaa
    39721 caatagattt cctcctttct ttcttactag tatatattat attcgttaaa aattctttta
    39781 ttcttttttt
//
LOCUS       DACDIG010000021        38976 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.021, whole genome shotgun sequence.
ACCESSION   DACDIG010000021 DACDIG010000000
VERSION     DACDIG010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.021"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(554..2167)
                     /locus_tag="IV247_001131"
     CDS             complement(554..2167)
                     /locus_tag="IV247_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAP8777213.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2308..3216)
                     /locus_tag="IV247_001132"
     CDS             complement(2308..3216)
                     /locus_tag="IV247_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8777214.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            3399..4523
                     /locus_tag="IV247_001133"
     CDS             3399..4523
                     /locus_tag="IV247_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8777215.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            4750..5667
                     /locus_tag="IV247_001134"
     CDS             4750..5667
                     /locus_tag="IV247_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8777216.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            5660..6493
                     /locus_tag="IV247_001135"
     CDS             5660..6493
                     /locus_tag="IV247_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8777217.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            6576..7694
                     /locus_tag="IV247_001136"
     CDS             6576..7694
                     /locus_tag="IV247_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP8777218.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            7688..9586
                     /locus_tag="IV247_001137"
     CDS             7688..9586
                     /locus_tag="IV247_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAP8777219.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            9580..10803
                     /locus_tag="IV247_001138"
     CDS             9580..10803
                     /locus_tag="IV247_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAP8777220.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(10889..12823)
                     /locus_tag="IV247_001139"
     CDS             complement(10889..12823)
                     /locus_tag="IV247_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8777221.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     EIEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            13049..13705
                     /locus_tag="IV247_001140"
     CDS             13049..13705
                     /locus_tag="IV247_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP8777222.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            complement(13779..14132)
                     /locus_tag="IV247_001141"
     CDS             complement(13779..14132)
                     /locus_tag="IV247_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP8777223.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            14324..15253
                     /locus_tag="IV247_001142"
     CDS             14324..15253
                     /locus_tag="IV247_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAP8777224.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            15264..16100
                     /locus_tag="IV247_001143"
     CDS             15264..16100
                     /locus_tag="IV247_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAP8777225.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            16348..17124
                     /locus_tag="IV247_001144"
     CDS             16348..17124
                     /locus_tag="IV247_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8777226.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            17300..18043
                     /locus_tag="IV247_001145"
     CDS             17300..18043
                     /locus_tag="IV247_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8777227.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            18036..19616
                     /locus_tag="IV247_001146"
     CDS             18036..19616
                     /locus_tag="IV247_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAP8777228.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(19867..20346)
                     /locus_tag="IV247_001147"
     CDS             complement(19867..20346)
                     /locus_tag="IV247_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAP8777229.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(20362..21114)
                     /locus_tag="IV247_001148"
     CDS             complement(20362..21114)
                     /locus_tag="IV247_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAP8777230.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            21231..21301
                     /locus_tag="IV247_001149"
     tRNA            21231..21301
                     /locus_tag="IV247_001149"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21263..21265,aa:Gly,seq:ccc)
     gene            21667..22392
                     /locus_tag="IV247_001150"
     CDS             21667..22392
                     /locus_tag="IV247_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAP8777231.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            22646..22912
                     /locus_tag="IV247_001151"
     CDS             22646..22912
                     /locus_tag="IV247_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAP8777232.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            22909..23340
                     /gene="ruvX"
                     /locus_tag="IV247_001152"
     CDS             22909..23340
                     /gene="ruvX"
                     /locus_tag="IV247_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAP8777233.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            23366..23671
                     /locus_tag="IV247_001153"
     CDS             23366..23671
                     /locus_tag="IV247_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAP8777234.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(23756..24901)
                     /locus_tag="IV247_001154"
     CDS             complement(23756..24901)
                     /locus_tag="IV247_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8777235.1"
                     /translation="MATIKQYEKKDGTKAWLFQTYLGISPLTGKEVRTTRRNFKTKKE
                     AQIELNRLLVDFEENGLKKETNETFKEVYDLWYESYKTTVKEVTQLKIEIKFNKWILP
                     KYGQFRIKEITTKHVQKILNQWAKTTDQYKVLHSTASRIFKYAMTLGIITKNPCELVI
                     MPNRNVEKKKDTVKIYSRDQLESLFQYLESKRSTYRADYDKTLLRFLFFSGCRISEAL
                     ALDWSDIDFDSNTVTINKTLSQTKYGYKISSPKTRNSYGSLSLDNKTINWLKKWQINQ
                     KRYMLTLGITDPTMIFCGIYKEIITKHAIYSRMITICKNSGIPFLGNHVTRHTHASML
                     LESGVSMKEVQERLRHSSIKLTMDTYAHLTKETKEKTVEKLAEHLNF"
     gene            complement(24952..25716)
                     /locus_tag="IV247_001155"
     CDS             complement(24952..25716)
                     /locus_tag="IV247_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8777236.1"
                     /translation="MNLNKTSVGNRIRLVRKELGLSMKEFGKKITPTAADSIVSRWER
                     GISLPNNDRLTQIAKIAHKDINWLLWGTFKEFISSLLDKEGYTNFLNDFPETVDTIYN
                     DLMPISENLQFPDYENSILRLFNKIYLPIFNDYCLDIIELDLEDFINKRHSISSDKFK
                     NRFLSSFHEGVSNSGYKYGDTDLIYKYGIALLEKFDDSYKLNNPYESIDAYFSAMTVN
                     TEDTEKLIYTICKSLGIPFQKGSNTAQILSKNHNNF"
     gene            25785..26078
                     /locus_tag="IV247_001156"
     CDS             25785..26078
                     /locus_tag="IV247_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8777237.1"
                     /translation="MTDNEINAILSLVKTKKDEVVRLDSPVRKYRRFCGYRQKELAEL
                     LEISELTYRNKEKGRVPFKDYEIERIVKLFHKDGIHLDIVDIFFSDLKTKKDE"
     gene            26091..26375
                     /locus_tag="IV247_001157"
     CDS             26091..26375
                     /locus_tag="IV247_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8777238.1"
                     /translation="MDVSSVQVILPPDLDQSLRKYFYQVAKEAVDQVRIETSLSKEIF
                     RLGEACEYLSCSRSTLKKFINQRGLKVSIIGDCQFIRKEELARFIKEYEI"
     gene            26565..27530
                     /locus_tag="IV247_001158"
     CDS             26565..27530
                     /locus_tag="IV247_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HAP8777239.1"
                     /translation="MKTLFDYILTLTGGSVEFMAEKRMLSKTFLNNASFMKMSDSAKL
                     LYVYLNVNADDDGIVEAYPYVRMLGTPDDDVKILLAKEFLYMLNEDLLVFLPHWLQNQ
                     TLRTTRYTPSIYRFKLLNSNPDFFTRLKQISIEKILKAGQTEMLRNDLIEKWYPVGLP
                     KDDHGHAQVRLGQVSSDQSRSGKVSLGEIREDQSINLSDDMEVEIMRQKIKNQIGYNA
                     LKSNPCYKRIVEDIVNIIQSIYSEKSKTYRINREEKSANEVIEKVEQLNQFDIESAID
                     SFNAIDHEIHNLNLYWITTLYNCKNTVSSRAYNNFVTQTREVLYD"
     gene            27523..27741
                     /locus_tag="IV247_001159"
     CDS             27523..27741
                     /locus_tag="IV247_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777240.1"
                     /translation="MIENRLIDQICCLDQNVDTLELIESICFNTVVLDLRAENDNCVT
                     HIIINQEMAQKLSESLKKWAEGGNNENN"
     gene            27728..30418
                     /locus_tag="IV247_001160"
     CDS             27728..30418
                     /locus_tag="IV247_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777241.1"
                     /translation="MKIIKKKIAGRKINWLALSLLALMFIVIFTGNVAHASIFNWFDD
                     TESQTEFLNNPVYQPYLKKYNGNFYSQFGLWIGWSVIKSMFAIVDGIQNMIPEVLDLF
                     SFVESAGLNKVYQSVLSTIVVGLMILSLVIIGYKMVTGKGTVDLKSVGINVLMSIVLI
                     LLMPTLIGSTEKLSGIMFAKTLYNDATSINNQDGGVAWSLIEQGVTDLAYINKTDQYS
                     MIDKTNDRNNLTKENFWKTDLTQVLTDNVIDKMEKENSTADNLRYELVENSDNEFVAT
                     KFSDNFLSTFVDGIKSGYYRYQANLWGISIGLIALAIAYVFSAFVIITAILELAFKRV
                     LGVLVFATDLETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNSGQGISTNIF
                     IKTIAYICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRA
                     KGSGNGNGSDSGEGEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMAS
                     ERAIKPFKSMRDMANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPT
                     RLSDRKDGAKVENADRIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMH
                     GQAKSAEELINQKRQGAKYTPEAMNREEMLRKRVEGRTGANMDEKEALTKERIQEAKN
                     SNTGLEKLVKENVQNSTSSQGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNGQGT
                     KTIDVRENLQSSSTSQPKTVDVRENVQNSTSSQGFKSVDVRENVQSSSASQPKTVDVR
                     ENVQSSTSSQGFKTVDVRENAQKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDS
                     EIRENDKGSKPRRRFTYEDNELFRDTLNDPNPLFDSLLKK"
     gene            30432..30617
                     /locus_tag="IV247_001161"
     CDS             30432..30617
                     /locus_tag="IV247_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777242.1"
                     /translation="MQLILQISTHDFLELDELCESVKKATNEKITVTNYIELMIQEEL
                     KMKNELMNELSHIQKGA"
     gene            30621..30959
                     /locus_tag="IV247_001162"
     CDS             30621..30959
                     /locus_tag="IV247_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777243.1"
                     /translation="MARKKQKSIADLKKELAELERKLYPELGKFAYEKTGISSVKEFE
                     ETFEINQKGTREKWQKAVNFANSEFTLVHEFLASDQLLETKFQQFLRRKGETAYRIFY
                     REETQEESVR"
     gene            31108..31605
                     /locus_tag="IV247_001163"
     CDS             31108..31605
                     /locus_tag="IV247_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777244.1"
                     /translation="MAIDVSKLPLEERRKRYKRNEDSRRQNRPIKFRVDDYQYQELEK
                     TADIFGLSVPMYVKKIAVDNAGKSLKYTDRQYKEINFHLLAIGRNLNQLVKTANYLRV
                     QLNKKDTEIQKEEHQSVYLSFTTKQERLKRLYAKKNDLMIQIKSISDPLNRLEKELNK
                     LWARL"
     gene            31590..32927
                     /locus_tag="IV247_001164"
     CDS             31590..32927
                     /locus_tag="IV247_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP8777245.1"
                     /translation="MGSIIKIASKSRSIHNTISYMLNKQEDSELVTGIGVSNDLNAIE
                     DFKRTNEIFGDKGKIKLWHFTQSFDPLEDISAEKAHELGVKWAEKMFPNQSILVVTHT
                     DKGHVHNHFGISPNDQRTGKKMDIRKNDIRVLQAWNDNILIDNGISPLDMYKYNHLVA
                     DRENWKEREFRTENKISNREKIKLAVSEVAQYAKNYDDFHAELKKKHSVETYRFAKNQ
                     RIGYKLLDSEGGLILKIGGKRLGQNYDMNYLDDLFTENKKAEQIQSEEVLTEIAIRHL
                     GKSLNGRADSRINWQERAQRFELDNENIPFLRSSKDYEEFYDKYKFEIEDRIEIVLDV
                     FGENKDQFFGFAVEEEPLKYKKKCVDLVYKKVVYDISESLSTDYDQFNVHDTKPALER
                     NFKKRYIQETKRVLYLFNRAIDDGTIEKARREYEETAREIDYQKRQNQYDMDF"
     gene            32949..33263
                     /locus_tag="IV247_001165"
     CDS             32949..33263
                     /locus_tag="IV247_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777246.1"
                     /translation="MNYPYFKVSASEETKEIFNNFYNQNKGVFGSKANMFRVMVSNLP
                     VLASPSNNKFNDSESIKFEQKISELESMISNEVIEKLDDIDQKLSYSLKNKYKTEEKK
                     DV"
     gene            33256..33750
                     /locus_tag="IV247_001166"
     CDS             33256..33750
                     /locus_tag="IV247_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777247.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTIDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLKNDTEKPIRPQQW
                     SIENKDGEELYEYAMILDNPEYIESDKAETINYYYELPNEMQDYQIVLRRANGYLVGL
                     PFTL"
     gene            33827..34141
                     /locus_tag="IV247_001167"
     CDS             33827..34141
                     /locus_tag="IV247_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777248.1"
                     /translation="MAEENKVVPFETLVINKEEFEQLFLNFFIQYDRIYDFSSSLDLF
                     ELEAEIIENSREKNGKIIISVEKFNELFSETFEIDPDYYQPQEVFREFRNQIIRSSNI
                     EN"
     gene            34405..35454
                     /locus_tag="IV247_001168"
     CDS             34405..35454
                     /locus_tag="IV247_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HAP8777249.1"
                     /translation="MDKIFKIFVSSTFTDLEDVRLQAMQGVLKAGHMPIMMEGFAATA
                     TQKEIIKKKITSCDAYMLIVGSKYGTIDPESGLSYTEWEYDYALDNGIPIYTMILEEK
                     YIQERMANGLLKLSDIETTSENYKAFVSKASSRQVDFINSIEQVKSMTEAGIMQLVKD
                     FGSSMIGWVSATIVDNVKMLENELDNLRKTRSELQVKLTNAETREIRESELPPNSSFD
                     GLLSKMFAPNSTDPILEQGMIYIMEFIREKVEQLKATIESHRGNIALGYDANKKDQVL
                     LRLGKDCLFFLADYNKAIINVRFAIQDPYFSENIDTLHVKNDVLYSEKYSEPLSLDYL
                     NKTLSQYHEIKKQQN"
     gene            35817..36653
                     /locus_tag="IV247_001169"
     CDS             35817..36653
                     /locus_tag="IV247_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAP8777250.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            36717..37148
                     /locus_tag="IV247_001170"
     CDS             36717..37148
                     /locus_tag="IV247_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8777251.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(37210..38532)
                     /locus_tag="IV247_001171"
     CDS             complement(37210..38532)
                     /locus_tag="IV247_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAP8777252.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(38686..>38976)
                     /locus_tag="IV247_001172"
     CDS             complement(38686..>38976)
                     /locus_tag="IV247_001172"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="malic enzyme; oxaloacetate-decarboxylating;
                     NAD-dependent; catalyzes the formation of pyruvate form
                     malate; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAP8777253.1"
                     /translation="LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAIL
                     PPVAKITEFSQTIAETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
BASE COUNT        13097 a         6202 c         7669 g        12008 t
ORIGIN      
        1 tgttaaataa ataggaacaa aaaaattaaa ttatttaata cacacattag ggatcattca
       61 ttgaaggatt agaagacaac gaggatggcg aaaaatatga ttacaataga aaagtcgttc
      121 taaaccaatc agttcctagt atcacacaac gcaagccagt cgatttggtg agattagagg
      181 agtgaaagca aactttatta tataagaaaa aatattcgaa agggatgcta gtttaggtac
      241 tagtgctgat agattaatat gatgcctcgt aatattaggc gtagttactc tagagacatc
      301 actatggact atgatttgtc tcagagtcac aacatgtttt tttatgaaaa gaaacagcta
      361 aaataattaa attgcgcaat gtttaaactt tcttaaacta taacctaata aatgtgaaga
      421 aataatgcac aatttcttca catttatctt ctattctata tacataccaa ccaacaaccc
      481 tatatacttt ttcattttta ctcctcaagt aaaaataact cctagaccgc ggctacccac
      541 cgcggtcgtt tttttattct tcaatcaaaa tcgctttgat acccaaacct gccaagattc
      601 gggcagaagt ataagtattc aatcctcttc tgaaagtaat gtaaactttt ttatcttttg
      661 ggatttcatt gattcgatct cgtaactcat tcattggaat attttttgtt gccgagatac
      721 tgccagaagg tgctttatta ggttcacgta tatctaaaaa gaacgcttct ttcaaatcgt
      781 tttcgggtat atcacttgct ttgatcgttt ctacgatatt cgtcatttga ttgattgcga
      841 cataaccagc tatattcaac gggtcacggg tagtagaata cggtggagag taaggcaact
      901 ccaaatcagg caaatcaaag actgtcaagt ttccggtgat tgctacagaa agttctccca
      961 tccgtttatc gacacctttt tctccaaatg cttgtccacc taaaatacga ccgctatctg
     1021 catcaaaaat cagctttaga tttaatcttg tagcttctgg atagaaatag gcatggtcaa
     1081 agggagtgat aaaaacagtt ttataatttg ttatgcctga tgcttttaat tcatgttctg
     1141 tcattcccgc ataacttgct gtatgatcaa agatttttgc gacacctgca ccaatatatc
     1201 cgcgatagcg cataggcgta ccatttaaaa tatctgccag catatgtcct tgacgattgg
     1261 ccgcactgga aagcatactt tggatcggct gaccagtcac tacactagtc gtttcgataa
     1321 tatcgccaat cgcatagatg tccggcagat tggtttgtaa ctggtcatca acaatgatct
     1381 gacctgtatc tgataattgt atgccggttg ctttcaccac ttcattcttc ggagaaacac
     1441 caacagcaaa aattagcata tcagcagaaa ggacggagcc atctgataat actatttctc
     1501 gacctttatc acggatctca gtaactcttg tttctaaatg aacggctaat ccttctttca
     1561 gcaatttgtc ataaactaaa ttagcaattt ctggatcata agggaaagcg acttgtggca
     1621 attgatcgat taaagtcacg tctaagccgc gatgcttgaa attctcagct aattccagac
     1681 ccataacacc tgcaccaaga atgacgacat gttgtggatt tttttcttca aggaacgatt
     1741 tgattcgatc cgcatccgtc acactacgga ggacaaagcc gttttctgct tcaaaaactc
     1801 cgggaatatc cggataatct ggtctagcac cagaagagat gatcaactta tcgtaatctg
     1861 tttttatttc ttcatttgtg gataggtctt taactgttaa tcgcttagta gaaggatcga
     1921 ttgctgttac ttcgtgttta gtaaagacat cgatgttgtt ttttgttttc aatatttctg
     1981 gtgtacgttc gatcagcgag tcacggtcag tgatcacacc tcctaaataa taaggcaaag
     2041 cacaacttgc ataagaaata ttttctccgc gttcatagat aatgatttcg tgcgcttcat
     2101 tcaatcgacg gaaacgagtg gcaaatgaag gaccgccagc tacaccgcca atgacaacaa
     2161 ctttcataaa aacatctcct tttctacgtt gatcgtacta tgttatggta aaaaacagca
     2221 aataattgtg ccggaaaatt attgtttatt ttgaatagac agaaaatata gagaaataat
     2281 ttgtggataa aacctaaaaa tttttttcta ttttaaggat agataatact ttgaagggga
     2341 taaccctgta tattttttaa aagctgaaga aaaatgatgg atcgaatgaa atcctaatat
     2401 atctgcaatt tctgtatagg ttaattcatt ttctagcacg agacgttttg ctttttccat
     2461 ttttagttct ttaaaatagc taataacact ttttccattc acttttctaa aatgtttttt
     2521 taagctggct ggactgataa aaaaaatttg ggctaattgt tcaagcaaaa aattttctga
     2581 aagatgttct tctaaatatc gtatgattgg atcaactaat tcatctgaat aaagaatatg
     2641 ctttttttta ggaatgactt tctcatttga aggtattgtc aataaagcaa gttcctgttg
     2701 ataatgcctt ggtaaggaag acaattgaat tttttgcaga caataagtaa ctaataagga
     2761 aagcaaatga atgctcatct gttggcatag tatgaaaaaa gaattgattt cctgatcata
     2821 ggaaatagct ggcttactat gttttagata atcaattttg aactgttcat ttttcagaaa
     2881 agtaatgata gatgttattt cttgatctat agaaaaagat tgtcccgtaa tatgtgctaa
     2941 taattgctca tcaaaataaa actggataat gataaaagtt tgtgtttcct tttgagtaga
     3001 aaagatttgg acttcttgct gtttatcaat gaataaaagg gaaggattat ctagttctat
     3061 agatttatgt gcaaattcag catataaatt agcgctttca tttattagta gtcgaaaata
     3121 gggagcagca gataaatgaa gatgggtatc tatggaatag gttagtgttt ttttgacaag
     3181 attggaggat tggttgtttg ttaacaaaag aaccactttc ctttctcttt ttatataaaa
     3241 agctttctat ttataattaa tgactattat aacaaaaatg aaaagccaaa acgacaagta
     3301 aaatattttt ttatataaat atttgtgtta ctaattccgt taatatacat aagtgaaagc
     3361 gttttatata agaacttgaa aagaaagaag ggatagacat gaaaaaaaga tacaaattgg
     3421 tgatagcttt gctaaacatt accctattat tcagcttaag tgcatgtgga aaaactaacg
     3481 aagaagtaac agcaaacatc gcagatccaa tgataaaaaa agccactaca ttagaagtat
     3541 atgtcgcaaa cggcaaactg acaaactatg ttgaaaatgc tgcaaaaatc tataaccgca
     3601 aaactggagc aaatattgac ttaaaaattg tgaatgtagc ttcgggaaca gcaacagtac
     3661 aaatgatcac cccaaaattg gtaacacacg aagatatgcc ggatattatc tccatggcag
     3721 acacttcagc agcaggtgtg ctggaaaaat ttcaagatgc tttttatagt gctgatgaat
     3781 atggatttta tgacaaatat ggcgatcagt tttatgatca aaaattgaat attttgcgtc
     3841 aacaaacgag taatgaaacg attattcctt gggctagtga ttttacccca gcactttgtt
     3901 attatcaacc aaaaaaattt gctgaaatag gtgtagattt tgaagaaatc agttcttggg
     3961 aagaatatat ccaggtggca aaagcattaa aagaaaaaac tggtagtttt ggaattgcct
     4021 tgccagaatc tggggaccaa gaattgttta tcaacatgct agcccagcag aatcaatctt
     4081 tgcttgatga agaaggaaat atccgtctga atactaaaga agcgaaacat gctgcaaagt
     4141 taatcaaaga aatgatcgac tcagatattg tacatttcta cggtgcacaa gatgctgaga
     4201 aagcatttca agaatctgca atgtttgttg ctggcggctg gtatgctacg aatatgtctt
     4261 tgaattttcc tgatgcatct gacaaatggc gcatggctcc actgattcct ttttcaaaag
     4321 aaaatccagg aaaaggtgca gtatcaggag gatcaagttt ttatgtccct aaagatgcca
     4381 aaaatccaca agtggcacaa caatttctta catttatgct gacagatgaa gaatgcttag
     4441 ccaacgcact ggagctgggc gtggctacat ctaactcatt ggcctatcaa acagaagcag
     4501 ctgagaaaaa gtttgactac taaggaggac aaaaatacta tgaactattg aataaaatca
     4561 atgaaaatac atcaaatact gttttaccag ccacttattc agatgctacg tcttatttga
     4621 ctaccgctgc atataactat tggcaaacag gtgattttga aaattcatat ctaaaagaag
     4681 cagaaaattt tgctcataag tacggcgtaa aattaactgg tgattaaaaa atattaggag
     4741 gacttaacga tgatgcaatc ttcgttacca agcaagacaa taaagaacag caagaaaaaa
     4801 gcaacagata cgtctcataa aaaaagtgct tatggattgg tcacaccttt tgttattttc
     4861 tttattgtga cgattttgat tcccatcatt atctcgtttg cttttagttt tatgaaaatt
     4921 ggctactcat ttgaatttgt tggatttgac aattatctag ctatcttccg agatccattg
     4981 tttattaaat cttacacgaa tgttttgatt atcatggcag gatcaattcc aataacgatg
     5041 ctattagcta tttactttgc tgttttattg aattcagcac agttaaaagg aaaaggattt
     5101 tttagaactg tctattatat tccaaccatt acatcaacgg tggctgtagc ttccgtattc
     5161 atgacttttt tcaatccgac tgggttattc aatggatttt tgaatgcgct aggaattaac
     5221 ggtgtacagt ggttaacaaa tcctttttgg atacgaatat caatgatcat cacgatgatt
     5281 tggcttagtg taggatataa cactatttta tttttagctg gtcttcaagg gatttctaaa
     5341 gagatatacg aaagtgctga aatagatgga gcatcgaaat ttcggcaatt ctttttatta
     5401 acgatcccca tgctaaagcc aatcatttta atggccacag ttttagccac tatcaatggc
     5461 ttgggtaatt tcaatatccc aaatatattc tttgggactt ccaatggacc agaaaattca
     5521 gctttggtcg taggagtcaa tttatataaa acaagttttg aaatggttga ttttggaaaa
     5581 gcctctgcta ttgcctggac gatggtgttt gtatctgtta tattatcggc tattcaattt
     5641 aaattcggag gtgataaaaa tgactaaaca aaagatacaa agcagaattt ttacgtacgt
     5701 cattattgtg tttggtgcct taatattttt gatgccatat ttatatatga ttgtagcttc
     5761 aacgcaaagt aatcaagaaa ttttaagtgg cggaatcaat ttcaagtttg gggatatgct
     5821 attaaaaaac tggcaagcca tcatggcgag atataattat cccttgatta tttggaattc
     5881 gattgttatt gctgtaatca gtacagtttc agctacagcc agttctacaa tggcgggtta
     5941 tgcattagca aagtatcgtt ttatggggaa taaactgttg ttcaatacag ttatgctatc
     6001 taggatggtg ccttttttta ccacattgat tcctttattt tacattatga gtcgtttagg
     6061 attatcgaat acttatgcag gtgtgatcat cccttcgatt gccagtacaa cttcagtgtt
     6121 tatgatgcga caatatagca gtcaatttcc tacagcactg atggaggctg ccagaatcga
     6181 tggtgccaat gagtgggtga tttttacgaa aatcgctgta ccgattttat cacctacgat
     6241 tattactagt ggtttactta tctttatggg aagttggaat caatatctat ttccattagt
     6301 catgctttca gataaagata aatatacggt tcccttaatt atcaaaaata tgacggtttc
     6361 tagtacaggt gagccaatta attatggcgc aattatgcta attttagcaa cttcagtgat
     6421 cccaatggtc ttgatttatg tgtgggcgca gtctaaattt aaggagaatg acattggagc
     6481 tgcaaataag taaaaaaaat aacgaatgtt aaccatctag ttcgttctag gagcaactcc
     6541 acattcataa aaattaaaag aatggcggga tatccatgaa tgaacagacg aaggaatggt
     6601 caagaaaaat tttgaatgta gcttctgaaa agatagatgc ggaattagac agaatgggga
     6661 ataaaattcc atatatttct tacaacggaa aatatacaga cgtaataaag gacagaggaa
     6721 tcgactggtg gaccaatggt ttttggagtg gtattttatg gcagctcttt cacaagacaa
     6781 aacgagaaaa gttcaaagag gcagcaatac aacaagaaca attattagaa tctgccctgt
     6841 accatttttc agacgttcat catgatgtag gttttttatt cttaccatcg gctattgcac
     6901 attatcaaca gtctcaaaac aaacatagtc gacaactagg gttacatgca gctacggtac
     6961 ttgctggacg ctataatatt tcagggaact atttagtagc atggaatgac cataaacctg
     7021 gttggatgat tattgatagt atgatgaatt taccattact gtattgggct tcgagggaaa
     7081 ctggtgatcc gaggttctct aaaattgctc ggcagcatgc agatacaaca gctaagtatc
     7141 tagtgaggaa agatggatca accgggcata tcgcagaatt tgatgcagaa tcagggaaat
     7201 atattcggtt acttggcggg caaggataca atgagaattc ttgttggagc agaggaaatg
     7261 catgggcttt atatggattc gcgttaagtt atcgttacac aaaaaatccg tactatctag
     7321 ctatagcaga aaagatagca gtaaatttta ttcagaaaat cgaaaaaact ggttatattc
     7381 ctctcattga ttttgctgta aaagattcga atattgatac cagtgctgga gtatgcgctg
     7441 catgcggtct tctggaatta gcaggatatg tttcctctgt acgatcaaaa atgtatcatg
     7501 aagctgctat caatatactc caaaagacag aaaagcacta tgcaaattgg aatatggatg
     7561 aagatgggat cattggaggc ggagcagaag cataccatag agaactgact tataatgtgc
     7621 cactgatcta ttctgattat ttttttattg aaggcttact tcgattagaa cagtcggaat
     7681 tagctatttg gtagtaaaaa gaggagaaaa tacaatgaaa catccagtac cactattaag
     7741 gaccagacag gattactttg acttatttaa aaagataaat caaccactcc agccgtttta
     7801 tcgagcaagt ggcgcacaaa ttgattatat acatcaaggg gtaggatatg gaaataaaat
     7861 tgcaggaatg gaaggattct ctcgggtttt atggggagcg gggcccgcaa ttgaccaatt
     7921 agatgaacga tggttgaaat taattctatc aggaataaaa gctggaacag atccttccca
     7981 tcccgattat tggggagaaa tacatgaaag agaccagcgt atggtagaaa tgcccgcaat
     8041 gttattagcg ttatatcatc atgaacaatt attatggaaa aaattagata caggagaaaa
     8101 aagccagatt attcattggt tctcgcaaat ttttgaatat gaatgtgcag atggtaattg
     8161 gcaatttttt aagataattg tcggaacaat tatccaaaaa tttggttatc cagttaaaga
     8221 atcagcactg aaagaagcat ttgataatat agaaaattgt tatcttcaaa atggctggta
     8281 tcgagacagt tcaagaggtc gccaagatta ttataatcct tttgcatttc attattatgg
     8341 tttgatttat agcgtactag aaccagaaaa gcccattagc cagatatata agaaccgagc
     8401 tattgatttt tctagagact atattcattt ttttgcggaa gatggtgcaa atgtgccttt
     8461 tggtcgaagc atgatttatc gttttgcagt ttcaagcttt tggtcagcta tgttgtgggc
     8521 cgatcttttg ccttttaaat taggtgaaat caaggggtta gtcaatcgga atataagatg
     8581 gtggatgcaa aaagaaatat ttgatgaaaa aggtatcctg aatattggtt atacctaccc
     8641 tcaattgaca ttgactgaac cctataatag tacgttatct ccattatggc tgaataagat
     8701 ttttttattg ttggcgctac ctgaagacca tgagtattgg acggctaaag aaatgcctat
     8761 gcctattcgt gaaggaacta aacttctttc agaagccaat ttgctcatac aacatgataa
     8821 tggacataca ttttttttga atgcgggaca attagggcca aatttccata cattgaccaa
     8881 tgaaaaatat ttaaaatttg cttattctag tcaatttggt ttttcgatac cgcgagcgaa
     8941 tcaattgaaa gcagaaatgg caatggattc aatgctaggg attcagcgag cggacacata
     9001 tatcacgacc agttttaaga atgagagcat caaaacctat ggacagttca tcgttcgaaa
     9061 taatgtacga gacattgtat gtaagaaaga ggtacttgcc tctacatggg aacctacttt
     9121 acaggcaaaa atcagaactt ggcttattcc ttttgaaggc tggcagattc gcgtacacaa
     9181 agtagaatta gatacagaat atgttctata tgagacaggg tttgctattg aatgttctcc
     9241 tgagaaagaa ggaataatca tagaagaaga caaggaaaac tatagggtga gtgaagctgg
     9301 cttttcaggt attatctgtt taagtgacga tactattaaa cgaaaatcca cagctattat
     9361 gggtttgccc aatacaaact tgatgacatg ggaaaacact tttattcctg gattagaggg
     9421 tacatttgga aaaggaaccc attggctcgg aacaggagtg gttgctcatc aagatcggga
     9481 ttatagttta gaagtatgga acaatcgtcc caaaattgat tttgatggga caaccgggct
     9541 aactatccaa aataaaaaca ataaaaaaag actaaaatta tgctaaaggc tggtggggaa
     9601 atgcttgata aagtgatgtt tgaagagata atacatccag aaaaatggat aaatgaatgt
     9661 tataaagaat taatggaaaa agaatggaat aatgagatcg agcgttgtcg cagacacacg
     9721 gagaggttaa tcaaacgtat cagtaacagt cagtatcaat tcccaagcga gtgtcctata
     9781 aaaggacgtt atccattaag agacaatatt ggttggacag aaggattttg gacaggaatt
     9841 aattggttgt tatttgagaa gacgggtgat cctttatttc aacaaacagc tgaaaaagca
     9901 gatatagatt ttatccacag gattgactgt caaattatca ctaatcatca cgatcttggt
     9961 tttttgtatt ctttatcaac cgtagcagat tataaaataa cgggaaatcg aaaaatgaag
    10021 gaagcttcaa ttgatgctgc agaaacatta cttcagcgtt tttgggagaa ggcaggagtt
    10081 atacaagctt ggggagatcc tagtgatcct tcacaacaag gtagaatgat catggattgt
    10141 tgcatgaatc ttccattatt gtattgggcg agtgaagaaa ctggaaatca aaaatataaa
    10201 aaagctgctc aggctcatat tcaaaatgca gcagattatt tagtaagaga agatggttca
    10261 acttatcata cattttatat ggatgtcaaa gatggaaaac caagatatgg aagtacgcat
    10321 caaggttata gtgataattc ctgttgggct agaggccaag catgggggat ttacggtttt
    10381 gcattaagtt atcgttattt gaaagaacaa aaatattttg atttaagcaa aatattagcc
    10441 aattatttta tgaatcggtt gccagaagat accatttgtt attgggattt gatctttgat
    10501 gattctagca gagaagaaaa agatacatct agtatggtga tcatggtttg tggattatta
    10561 gaattaatag aatcaggtct attagacaaa acagaaaaga acttatacca gtctgctgtt
    10621 tggaaaatta tgcgacaatt gaaagaaaga tatgctacaa cggaagaaga agatggcttt
    10681 ttaacacagt ctgtttataa taagcccaaa aataatgggg taaatacacc ttgtatttgg
    10741 ggtgattact attatttaga agctttagtg agaatgacta gtaattggtc ttcttattgg
    10801 tgaaaaaggt aaatatggaa tttttgtttg ataaaataaa aaaagaaaaa ccaaaacagc
    10861 atgaatggtt tctgttttgg ttttttcgtt aattttcata ttcttctaat tccaagctta
    10921 gttcttccca ttccgtcaat tttgtttctt gatcttgtct agcagcttct aaatcttggt
    10981 tcagctcatt gagttttacg tgatcttcca ggttttctgg cgcgaccatt tcttgttcca
    11041 actgatcgat ccattcatcg atccgcgcca tttcttcttc aacctgagta atttttcgtt
    11101 gaaggctacg gataagcttt tgctgttctt tgctttggta ataatcagat ttgttcgtgg
    11161 gagcaggagc agattctggc tcatcttgtg ccaacaattc ggcgatttct tcttcttcct
    11221 gctttttctc taaataataa tcatagtctc ctagatagac tttgctgcca ttttcagaga
    11281 gttcgacgac ttttgtcgcg atccgattga tgaaatatcg gtcatgggaa acaaaaagca
    11341 gtgtgccgtc ataatcgatc aatgcatttt ctaatacttc tttgttgtca atatctaaat
    11401 ggtttgttgg ttcatccaaa atcaaaaaat tttctttgtt catcgctaac ttggctaaag
    11461 caacccgtgc tttttcaccg ccacttagca gagggattgg tttttctaca tcttcgccag
    11521 aaaataagaa agcacctaaa gcacttcgga tctctttttc tggagtggta ggatgctcat
    11581 cccacaattc tgctaggatc gtcttgtttg tatgcagatc agcttgacct tgatcgtaat
    11641 agccgatggt gacatttgtc cctagcgttt cttttccacg gataaaaggg atctggtgaa
    11701 tgattgattt cagcaacgtc gatttcccga taccattcgg acctactaga gcaatcgctt
    11761 cttgacgacg gatatctaga ttgattggtt cagataaggt gacattatca tatccgatag
    11821 cggcatcttc gatttgcaag actacatttc cagaaggctt ttctatactg aatagaaagt
    11881 ttgcttgttt ttcatcccct tgaggacgat ccagccggtc cattttttct aatgtctttc
    11941 tgcggctttg cgctcgtttt gtggtagagg cgcgaacaag gttccgagca acgaagtctt
    12001 ctaatttatg gatttctgtt tgctgctttt cataagcctt ccattcactt gctaactgtt
    12061 ctgctttcaa atctagatat ttcgaatagt tacctttgta atggcgcatt tttcttctgc
    12121 ttaattcata gacttcattg actactttat ctaaaaaata acggtcatgg gaaacaatca
    12181 ataatgcacc agtatagctt tgcagataat tctcaagcca ggaaagtgtc tcgatatcta
    12241 aatggttcgt tggttcgtct agaatcaaaa tgtcagggtg ttgcaaaagc atgcgagcta
    12301 aagctaaacg tgtgcgctgt ccgcctgata aagtatcgat tttctgatca taaaatgaag
    12361 aatcgaattt aaacccatgc aatacggaac gaatttcatt ttcataacca tagccgtttc
    12421 gatctgaaaa atcgtgttgc agccggtcgt attcttttaa ataagaacta taagcaggtg
    12481 tgtctggcgt accttcgcta atgaaaatct ctatctcgcg catccgctct tccatttgtc
    12541 taacttgttc aaaagctttc agcatctcgt tccaaactgt ttcattggaa tcaagtcctg
    12601 tgtcctgagc gaggtatccg agagtcgctg ttttattttt agcgatcttt ccttcatcag
    12661 gtgcttcaat tccagcaatg atctttaata aagtcgattt tccagcgcca tttctgccaa
    12721 ctaaagcgac acgagcgcct gtactgattt ctaaatgtat gttttcaaat aagacttctg
    12781 caccaaaatg gcgagcaatc tgattggctt gtaataatat catggaatcc tcttttctac
    12841 tttgtgaatt ctcctcaaat aaaaaactat ttttcaatta gtagtctacc ataaattcca
    12901 ttatactgcg agatttgcag aaatgataaa taaatgaata aagaataaaa tttcacaata
    12961 tctgagcaat ggaattgatt ttttcctata aatactgcta tgatggagaa atcaaagatg
    13021 taattagaca atacgtggag gtcatttcgt gaaagatcat attattccaa aggcgacagc
    13081 taaaagactg cctttgtatt atcgctattt aaggatttta aataatgctg gaaagaaaaa
    13141 agtgtcttca actgaattaa gtgaagccgt tcaagtagat agtgcaacga tccgtcgaga
    13201 tttttcttat tttggcgaat taggaaaacg tgggtatggt tatgatgtag aagatcttat
    13261 gcactttttt gcaaagacat taaacgacga tcaattgaca aatgttgcgc tgatcggtgt
    13321 agggaacttg ggaagtgctt tactaaaata caaattccat caaagtaata gcatacgtat
    13381 cagttgtgct tttgatgtga atgaggaaat cgttgggcgc atcgtagacg gtatccccgt
    13441 ttatccaatg gaagatatgt cagaacaaat caagatccaa caaatcgaag tagcaatttt
    13501 aacgataccc gctgaaagag cacaagaagt agtcaatcag ttaacggaag caggaatcaa
    13561 aggaatcttg aactttaccg ctgctcgatt gaatgctccg tcagatgtat tgatccaaaa
    13621 tgttgatttg acgaatgaat tgcaaacctt gatctatttc ttgcattctg acgcaaatgc
    13681 gcattccgaa aatacagcag aataaaaata aaaagcgatt gaacaaatga aggcaaaact
    13741 ttcacttgtt caatcgcttt tttctgtttg gacaacgatt attttgttcg gaagatttct
    13801 tcataagaaa gggaatcatc ttctgctgtc agttcatcta gtagataacg gattttttta
    13861 aagtcgatga accgtgcttg ccgaaagact tcttttcctt gatgaaaaat caggacaact
    13921 ggcgcagtca gcacttcaaa tttactagct acttctggtg tttcaaatgc atccaaatga
    13981 agtgccggaa tatcataatg tgtgagaagt tcctctagtc gtggacgaac agcgtgacaa
    14041 acactgcaat taggcatcga aagataaatg acggcaacgg atgactgttg cagtatgtct
    14101 tgaacttcag tcaagttagt ggtttgtttc atttttattc tcccttttat gatagaagtg
    14161 atatgtatga gaccttaatt taatgggaat aacagaaaaa gtcaattgaa gtgctttggt
    14221 aaaaaataag agttgtgtaa aaatttacag agcaacttgt tttttttgaa taaaagcgta
    14281 tatttgcaaa gcgaatgaac tagaaataaa aaggaggaaa ctcatgtttt cttttattcc
    14341 aaattcggtt ttgcagatga gtacgatttt cttatcgatc gttattgaag cattaccttt
    14401 cgtattatta ggctgtctta tctcaggcgc cttacaagta tttttgaccc cagaacgtgt
    14461 gaagagatgg cttcctgaaa atcgtatctt ctctattttt actggttcga tcttaggatt
    14521 tttctttcct tcatgtgaat gcggcatcgt accgatcgtt catcaatttg ttaaaaaagg
    14581 cgtaccagtt cacacagcgt ttgcttttat gctgactgct ccgatcatca atccaatcgt
    14641 gattttttcc acttttatcg catttggcaa ttcttggcag atggcaagct ggcgggtgat
    14701 aggcagcttt tttgttgccc ttgtgattgg tgtctggctt gcctattttc aaaaagacgc
    14761 tgtattgaaa gaaaaaatca cgtctaaagt agcagaaaat caccatcatc atgtccatga
    14821 agaagaacaa agtgagaaga atgaaccaaa aaatcagctg atccatcaaa ctgggcatgt
    14881 gttgacacat tcaatcgacg aattttttga tacaggtcgt tatttgatca ttggcggatt
    14941 aatcgcagct gcgatgcaga cttatttacc aactagaatt atgctaacgc tcggctcaac
    15001 aaagatttta gccattatca tcatgctgtt attggccttt acgatgtctc tttgctcaga
    15061 agcagatgcg tttataggat cttctttgtt aagtctgttc ggcacagcac ctgtagctgc
    15121 ctttttggtc tttggcccaa tggtcgatat caaaaatcta ctgatgatga aacgatattt
    15181 tacaggacgc tttattttat cgctgatcgg actgattgcc cttagcgtta ttggattcac
    15241 attatttatt taggagggaa aatatgctgc ggtttttgat tttatctggg tatttcgaat
    15301 tgatgatgta cttgcaagtt tctggcaaac tcgatcaata tatcaatgtc cattatcgct
    15361 acttagctat tctttcaatg attttatctg ctcttttggc tttagttcag ctgtatttgt
    15421 gggtcaaaaa cggaaaagca gaagaaaagg cacacgagca cgatcatgac catgggttgt
    15481 caaaacctta tcaacgtgga atcgcttatg tactgctagc tttgccagta atcgtgggga
    15541 cgttgtttcc aacagttagt ctcgacacta cgatcgtaga agccaaagga tttaatttcc
    15601 cagtaagtaa agaatcagta ggggatccgg agatgcagac acaatactta aagccggata
    15661 ccagtattta tttcaacaaa tcagactact tagaccaaat gaatgagttg aaagaaaagt
    15721 atggggataa gcaaactatc aagatcacag atgaaaacta tttagaagtt atggagctaa
    15781 tttataatta ccctagcgaa ttcattggca aaaaaatcag ctatgaagga tttgtttacc
    15841 aaacaccgaa cgatagcaat acagatatct tcttgttccg attcggcatt atccattgtg
    15901 tggcagattc aggagtcttt ggtttgttga cgcatatgcc ggaaggagta acggttaaaa
    15961 atgatgaatg gtataaaata gaaggaacca tccaatcaga ctattatcaa ccattcaagc
    16021 gtgaaatccc atctgtagtt gttacaaacg ctgagaaagt ctctgctccg aaaaatcagt
    16081 atgtgtatcg ttctttttaa aagaacaaaa taaataaaca tagctcagac agctgacacg
    16141 caaggacgtc ggttgtctgg ctttttttgc atgatgtatt catttgtttg atatttattt
    16201 tagaagcagc atcctgaaag tatttgatat cggtttcaaa ggtgttggaa aagattgtga
    16261 agagaattac aatttattcc taacatgtat gttgaaaagt tgtatgaaat gattttgata
    16321 gtaaaatatc gagtggaggt actttttatg gtaacgcgta tatttatttt ggatgatagt
    16381 ttggtttttg aaaaaatgct agtagaaatt ttggaagaat ccagaatttt attaagtcct
    16441 tggaaatttg agatcgttca gggattgaac gttatacaat ttgttgaatt cattaaaaat
    16501 aatgaaatct attcctcaga tatttttttc ctagacatcg atttgaacgc ctattatagc
    16561 ggtatcgaac taggcgaaat gcttagagaa aagaatgaaa agtgtaaaat tgtttatcta
    16621 acgtctcatc cgaataaagc aataactgtt attaatcgaa agattacgcc tagtggttat
    16681 ttgataaaag aattcactta tgaaaaaaat aaaaaaagca tccaaagtta cttattgaaa
    16741 gaaaaagaaa actatcctgt tcaacatgct acgaaaaaaa ggtggacaac tgtccgttat
    16801 ggtaatagcg atcactatat ccattacgat gaaatccttt atctaatgag catgccaggt
    16861 tataaaaata aaataggcct agtattgaaa gatgaagaga ttctcgtaaa cggcacaatt
    16921 aaaaaatata aaaaatcact cgaatatgag tatttatgta aagatttaaa agcttatatc
    16981 attaatactg aaaacattac atctattagc agagtaaacg aaatagtaac atttgcaaat
    17041 ggagaacaac tagagatggg aaagagaagt attgataaac taaagaattt tatgcaagaa
    17101 aagatagaaa atgaatcatt ttaaagaaaa caaagactca tatcttacct gttagaagag
    17161 tgttttcctg tagaagagaa caacccaagt agcagcctag tcgtttctat ccttttttgt
    17221 tatgcactct ttttgacatt tactccacca ataacgaagg gtacaataaa aagtaataac
    17281 aaacaaaagg agtacagaaa tgattgaatt catcaatgta agtaaaagtt acgggaaaaa
    17341 acaagcatta aaagacttgt ctctctcgat aagacaaggg gaaatttttg gatttttagg
    17401 acataatggt gccggaaaat caactacgat taaaagttta gttagtatta ttgaaccatc
    17461 atcgggaacg attttagtag atggaatgaa attgacggaa aatcgattga gtatcaaaca
    17521 aaaaatcggt tatgtgcctg attccccaga tattttttta caattgacgg ctggagaata
    17581 ttgggacttg atttcagctg cctatgaatt agacacacaa aaaaaagaaa agcgtttagc
    17641 agaattgaca gcgctttttg atatgtatag tcatcaaaat gaaacaatcg caagtttttc
    17701 acatggaatg cgtcaaaaaa cgattttgat cggcacgttg ctgccagatc cagatatttg
    17761 ggtattggat gaacctttgc aaggacttga tcctcaagca gcatttgatt tgaaagaaat
    17821 gatgaaggct catgccgcaa aaggaaaaac agtcattttt tctacacatg tattggatac
    17881 cgcacaacag ttatgcgata aactggctat tttgaagaaa ggtgaattga tttatcaggg
    17941 ggcagttagt gaattattga atggctcacc tgatgaaacg ctagaaaaga tttatttgaa
    18001 aatggctgga aggcaagcga caggagatgg accgtatgta taagcgacaa ttattagaat
    18061 taattcgagt taatctttta tatgttaatc cacagctcac gaataaagca agagagaaag
    18121 gaaaaaaggg aaagcgatta actatctatc ttttgtctca aaatcttttt tcagggctga
    18181 tttttttact gatttacgga tttgctatgt ttgctatgaa ctttgttaaa ttgccaggtt
    18241 tctttactta ttacgtggca ctttttggta tgttaggttt aactcaaagt atatcagtca
    18301 tttataatat cttttttgaa ggaaatgacc tgcaagtatt tttgcctctg ccttttggac
    18361 aagaacaggt tttttcagca aagattttgg tagttgcttt gaccatcatt ccttttgttt
    18421 ttccgcttct agtcatgttt attttaaccg gatggcgagc tgatctaggg attttgctta
    18481 ctatcattat ctcaatcctt ttgttttctc tatttttgat tattcttttt tgtctggcta
    18541 gtttgattgt ttttggtttg gctcgaacag cagtgtttaa aaaacataga aaaacagttt
    18601 ctagtatctt gcttggcagc tcgatgctga tcgcatttgc tggaatcatg tggatgaatc
    18661 atcaaacggg gactattgaa aatgagttaa cagatcggca tccgatttcg atattattgc
    18721 cattctatga tgctgccagt cagccacttt ctcaacaagg tttattgggt tggggaatac
    18781 tagctatcac ggctctggga tttttccttg ctgtaaaaca gctgatagta ccaaagctta
    18841 cagagcaact gactgattca aacagtgagt tcaaaataaa aagaacccat aaaaagaacc
    18901 aaaatgtgca tcagctttta tttagttaca atgctcaatt agtaaaagaa cctaatttga
    18961 ttatgcaagt tctttcttct tccttgttaa ctccaatcat ttttattctt gcttttgccc
    19021 taggaggcga aataaaatta actgatttag ataataagtt aatcggtgtt gttttcctgg
    19081 tagggatcgc attggctttc ttaacagtta atcagacttc ttttatcagc aatcttattt
    19141 ctttggatca agaaaatttt ctgtttatcc gttctttgcc aatctctatg aaccaatatt
    19201 taaaagaaaa gttcagattc ggatggatat tgcagagttt tttaactggt gggatcgcat
    19261 tattaagcgg actgtctttt cagctgcctt tgttttttat ccctagcttg ctatttggtt
    19321 ctttattcgg ctgctactta ttgtcactac gttatttcgc tagagattat cggttgctgc
    19381 tgctgaactg gaccaatatt aaccaattat ttaacagagg ttcaggaagt ttgggtttag
    19441 tcgcaactat gatgggttca ttgatcatca gtattattct tatcgtctta tacggaatgt
    19501 tggctttgtt ttatccattt tggttagtga atattcctgt gatcggttta gttctaatac
    19561 tttccgctct atggatcgtt tattatcaac gaacgttttg gaaaaaattt gaataaagaa
    19621 gaggggccgt gacaaaaatc atgtcgcagc ccctttttta cagatagagg gtgttcaaaa
    19681 gtcagtttct cagtattaag tcaattttat gtaaaaatgt gagaagctat ttttacaaaa
    19741 ctttttttaa tactcataat tacttcttat tgctcctgcg gttacttgtc gcaatgtttg
    19801 tgagatattt tgtcatcgct caaggctgaa cgtctctttc acaacctttt ctttgttaca
    19861 cttcgtttat ttaaaaagcc gtttttttcg tttcatcatg attgcgtcac gctttacttt
    19921 gaaattacgg atgttatggt taaagacttc tgataataca agacctaaag cgattgctcc
    19981 agcaatcata attgcttgga ccgcataatg agccgcttct cgataggatt cagtaacgag
    20041 atttcgaacc gcttgataag ctaaaccacc agggactaga ggaaccatcc cgggaatatt
    20101 gaaaatcgtt acgggcattt tcaaacgttt agagaaaaga tcgctgatga atgctactcc
    20161 taaagaacca agcaatgaac caaaagcagc agatccgcct atttgaacac agatccagta
    20221 gatcatccag ccaaaagcac cagaaagacc acaacaaatc aatgaacgcc taggtacatt
    20281 tgtaataacg gcaaatgccg cagtcgctaa aaaactaaaa gaaaactgaa taaatatcgt
    20341 ccccatactt gtgttccttt cctaataaaa aagctggaaa ataaaagcaa tcgcaaaacc
    20401 gatcatcgta gcagtcatca tcgcttctgt tccgcgagaa actcctgaga cataatgtcc
    20461 agctagcaaa tcacggatag cgttcgtaat ttgaactcca ggaactaaag gcatcacaca
    20521 tccgataatg attagatcct gattgcttcc taagccaaaa cgtacgctgt agatagcggc
    20581 tatgccaatc aataaagaag ataaaaattc cgataaaaat ttgattctaa aaaatttcaa
    20641 actatataga taagctgcgt atcccaatcc tccaatgact aaagtcacag gtaaatcggt
    20701 catctttccg ccaaataaca ccataattgt tccactgatc actgccgcgc taacgatttg
    20761 caaccagaca ggaaagaaag aacggtctcg gtcgattgct tgcagttcgc agtaaagttc
    20821 ttctaatgtt agctctgaag caacatattt acgtgaaaga tcatttactt ttactacttt
    20881 ttctaaatta atcgaacggt tggtaatttg ttccattcgg atggtcgagg tacgatcaag
    20941 accaataaag agaccggttt gagtgacata gctgaccaaa cggaaatttc cagaagctaa
    21001 agcaattcga ctcatcgtat cttctacccg atacatctcc gcattgcttt ccatcatgat
    21061 tttcccagcg agtaaacaag tgtccagcaa cagatctaat tcttttcccg tcatagcatc
    21121 ttctccaata attgattgat tagttttatt ttactacaaa acatatcgga agcgtaaaga
    21181 ataaaaaaac ttgaggaagg agtgaaagag gcgtatactg tttttgtatc gcgggtgtag
    21241 tttagtggta aaatcccagc ttcccaagct gatgtcgcgg gttcgattcc cgtcacccgc
    21301 ttaaaagagg ctgtgaaaaa gctgccctgc atcaagtaat aagaatagtc aagcaaaaaa
    21361 gcttcttatg tttttgactt ggttggactt attaccgcag atgcaagctt ttgaacactg
    21421 tttaccaaga ggtcgtacag aagccgttta gttttgagta ttaagaaaaa ttttttgaat
    21481 gataataaat accttgtaaa tagtgttttg tgattgagta gagacgatga atccgtctct
    21541 ttttttgtat tcttaagccg tctaaaaact ggttttattt ttttactgtt tctatttctt
    21601 tccagtattt atttataggg gaacatatgt taagatttag ggaactgtta gtaaaggagt
    21661 aaagaaatga gcaaaaaaac aaccaaggcg atcgtgggga tcgtcgctgc tgtgattgca
    21721 ggggcactcg gtatttccac cacacaaaaa gattcagata tcttccaaca aattacaacc
    21781 atttttgatt ccacaccgca agcttctcaa gtagcggatg tgccagaata tgatggaaaa
    21841 catcaagaaa tagaaatcaa tgggaatcag ccggagttta caaaagagga tcttagtctt
    21901 tccaaaggga catgggaaag ctattcaaat atagacaaat tgaacagagt aggacaagcc
    21961 aatgcgatgt tgggaaaaga cttgtttcct actgaaaaga gagaaccttt gtatattgat
    22021 ccgacaggtt ggaaacaaaa gaaattaagt gacggacaat ggttatacaa ccgcagccat
    22081 ttgattggtt tccagttgac tggtcaaaat aataacatta aaaacttaat gacagggaca
    22141 cgttcgttga atgctccgta catgcttgcc cacgaaaatg atattgcggc atatatcaag
    22201 gaaacaaatc atcatgtacg ctatcgtgtc actcctcatt tcgagggaaa tgaactggta
    22261 gcaagaggtg tgcagttgga agcagaaagc attgaagaca aaaaaattga attcaatgtc
    22321 tttatctaca atgttcaaga tggctacacc atcaattatg agacagggca agcaacgaaa
    22381 acgaaaaatt agtagcagca attcgaattt tttatgaact ttatgaaaaa attgtgtaaa
    22441 aacatacttt caaagaaata tagcgtatac tttatggaaa gagggctttt gaaaaagcca
    22501 cagaaaaaag gaaaaatcag acaaatcgtg tttcttaatt tattcagaag tttggaatat
    22561 ttgtttgtag ttttcccttt cttcgagtaa aataagggtg tagaaggtaa gctggaatta
    22621 ccttgcggga aagaggtgta gaatcatggg ttttacagat gaaaccgtac gttttggttt
    22681 tgacgatagt cgcaaaaaag aagtgagcga aacattagcg attgtttatc gggctttgga
    22741 agaaaaaggc tacaatccaa tcaatcagat tgtcggctac ttgctttctg gagacccggc
    22801 ctacattcct cgttatcgtg atgcacgaaa tttgattcgc cgtcacgaac gcgatgaaat
    22861 catggaagta attgtcaaag actacctctc aaatcatgga gtgaatttat gagagtaatg
    22921 ggcttggatg tcggttctaa gacagtaggc gttgctgtga gcgatccctt tggctggaca
    22981 gctcaaggag tagaaatcat tcgaatcaat gaggcagaag aagagtttgg ttttgatcgg
    23041 ttgaaggaat tagtggcgca gtacgaagta gaccgttttg tagttggatt acctaagaat
    23101 atgaacaatt ccatcggacc aagggcagaa gcttccatgt catatggaga gaagatcaaa
    23161 gaactattct ccttgccggt tgattatcaa gatgaacgac taaccactgt tcaggcagaa
    23221 cgtatgctag tagagcaggc aaatacttca agagcgaaac gaaaaaaagt catcgataaa
    23281 ttagcagctg tgatgatttt acaaaattat ttagacggct caggtaagca attattttaa
    23341 taataaaaag aaaagaggta atcttatgac agaacataac cattcccacg aacacgaagg
    23401 acacgaacac atcacattgg tggatgacca aggaaatgaa acattatacg aaatcttgct
    23461 aacagttgac ggacaagaag aatttggacg taactatgtt cttttatatc cagctggtgt
    23521 ttcagaagat gaagatgtag aattacaagc ttatgcatac atcgaaaacg aagatggaac
    23581 agaaggcgag ttagaacaaa tcgaaacaga cgcagaatgg gatatgatcg aagaagtatt
    23641 caatacattc atggcggaag aagaagaata agctttaaat ggtagctgaa cccttggtag
    23701 ataagggttc agcttttttg tattttgaat tgaatacgag attgaatacg ttccgctaga
    23761 aatttaggtg ctctgcaagc ttttcaacgg ttttttcttt ggtttcttta gttagatgag
    23821 cataggtatc cattgttaat ttgattgatg agtgtctaag gcgttcttga acttccttca
    23881 tggagactcc tgactctaac agcatactag catgagtgtg tctggttaca tggttaccta
    23941 gaaatggaat accagaattc ttgcagattg ttatcatacg agaataaata gcgtgtttag
    24001 taatgatttc cttgtagatt ccacaaaaaa tcattgttgg atcagtaata ccaagggtta
    24061 acatatatct tttttgattg atttgccatt tctttagcca attaatggtt ttattgtcta
    24121 aagatagaga accatagcta tttctcgttt ttggacttga aattttgtac ccatattttg
    24181 tttggcttaa ggttttgttt atagtgacgg tgtttgaatc aaagtcaata tctgaccaat
    24241 ctaaagctaa agcttcgcta attcgacaac cactaaaaaa tagaaagcga agtagcgttt
    24301 tatcgtagtc ggctctatag gtgcttcttt ttgattccaa gtattggaag agactttcta
    24361 gttgatcccg tgaataaatt ttgacagtat ctttcttttt ttcaacgttt ctattgggca
    24421 tgattacaag ttcacagggg tttttagtaa ttatccctaa agtcattgca tatttgaaaa
    24481 tacggcttgc agtagaatgt aggactttat attgatctgt tgttttagcc cactgattca
    24541 atatcttctg aacatgtttt gtagtaattt ctttaattct aaactgccca tattttggaa
    24601 gaatccattt attaaactta atctcaattt ttaattgagt gacttccttt acagtagttt
    24661 tatagctctc ataccagaga tcatacactt ctttgaaagt ttcgtttgtt tcctttttaa
    24721 gtccgttctc ttcaaagtct acaagtagtc tattaagctc gatttgagct tctttttttg
    24781 ttttaaaatt tctacgtgta gtacggactt ctttaccagt taatggactg attccaaggt
    24841 aggtctgaaa tagccaagct ttggttccgt ctttcttttc atattgctta attgttgcca
    24901 ttgtttttcc tcctatcagt tgcgtggcgt gcttttgata gaaaaaatga attaaaaatt
    24961 attgtggttt tttgaaagaa tctgtgcggt attgcttcct ttttggaatg gaatgccaag
    25021 cgatttacaa atggtatata taagtttttc ggtatcttca gtgtttacag tcatggcaga
    25081 aaaatacgca tctattgact catatggatt atttaattta taagaatcgt caaatttctc
    25141 aagtaatgca attccatatt tataaattaa atccgtatcg ccgtatttat atccgctgtt
    25201 agatactcct tcatgaaagg aggataaaaa cctattttta aacttgtcag aggagatgga
    25261 atgtctctta tttataaagt cctccaaatc cagttcaata atgtctagac agtaatcatt
    25321 gaatataggt aagtatatct tattgaatag tcgtaaaata gaattttcgt aatcaggaaa
    25381 ctgtaaattt tccgaaatag gcattaaatc attataaata gtatctactg tttcaggaaa
    25441 atcattcaaa aaatttgtgt atccttcttt atctaacaat gatgaaataa attctttaaa
    25501 agttccccat aataaccagt taatatcttt atgagcaatt ttagcaattt gagttagcct
    25561 gtcgttatta ggtaagctaa tccctcgctc ccaacgcgaa acaatactat ctgcggcggt
    25621 aggagttatc ttttttccga actctttcat ggaaagcccg agttctttac gaactaaacg
    25681 aatacggttt cctactgatg tcttattaag gttcatatta tatccctccg acagtaatgt
    25741 aacacaaaaa cggaatggaa tctacgaaaa acgacgggta agtattgacg gacaatgaaa
    25801 taaatgctat tctatcgttg gtaaagacga aaaaagacga ggtggtaagg ttggactctc
    25861 cagttagaaa ataccgcagg ttctgtggat atagacaaaa agaacttgcg gagttattag
    25921 aaatatcgga actaacgtat cgtaacaaag agaaaggaag ggtgccgttt aaagattatg
    25981 aaatagaaag aatcgtaaag ctatttcata aagatggaat tcatttagat attgtggata
    26041 ttttttttag tgatttgaag acgaaaaaag acgaatgagg agtgggcatc atggatgtat
    26101 cgagtgtaca agttattcta ccgccagatc ttgatcaatc attacgtaaa tatttttatc
    26161 aagttgctaa ggaagctgtt gatcaagttc gtattgagac atctttaagc aaagaaattt
    26221 tccggcttgg agaagcgtgt gaatatttga gttgtagtag aagtacacta aaaaaattca
    26281 ttaatcagcg tggtttaaag gtgagtatca tcggagactg tcaatttatc agaaaagaag
    26341 agttagcaag atttatcaaa gaatatgaaa tataaaaaaa gttgcgtggt gtgcttttga
    26401 tatgaaaaaa aggaagtgtt tgtttgaacg atgatgaaga agcaaagttg agtttaaaag
    26461 aaatcaagat ttattttcta tttgatttaa taaaaggtga atatacaaaa aaagagtgct
    26521 ccttacagtt tgcagaccgg aagaacactc aataataaag ttctgtgaaa actttatttg
    26581 actacatttt aacattgaca ggaggaagtg tggaatttat ggcagaaaaa cgtatgttgt
    26641 caaaaacttt tttaaataat gcgagtttta tgaagatgtc ggattcagca aaattattat
    26701 atgtatatct gaatgtaaac gcagacgatg atgggattgt cgaagcatat ccatatgtac
    26761 ggatgcttgg aacccctgat gatgacgtga aaattctttt agctaaagaa tttctctata
    26821 tgctgaatga agatttatta gtatttcttc cccattggct gcaaaatcaa acgttaagaa
    26881 ctacaagata tacaccctct atatatcgat ttaaattatt aaatagtaat ccagactttt
    26941 ttacaagatt aaaacaaatt agtattgaaa aaattttaaa agcaggtcaa acggaaatgc
    27001 ttcgaaatga tttgattgaa aaatggtatc ccgttggtct accaaaggac gaccatgggc
    27061 atgcccaggt tagattaggt caggttagtt cagatcagag taggtcaggt aaggttagtt
    27121 taggtgagat tagggaagat caatctatca atctatcgga tgacatggaa gtcgaaatta
    27181 tgagacaaaa aataaagaat caaattggat ataatgcgct aaaatccaat ccttgttaca
    27241 aaagaattgt tgaagatatt gttaatataa ttcaatcaat atactctgaa aagagcaaaa
    27301 catacaggat aaatagagaa gaaaaatctg caaatgaagt gatagaaaaa gtagaacaac
    27361 tcaatcaatt tgatattgaa tctgctattg attcatttaa cgcaattgat catgagatac
    27421 acaatctcaa tctttactgg attacaactt tatataactg caaaaatact gtaagttcca
    27481 gagcatataa taactttgtt actcaaacaa gagaggtgtt atatgattga aaatcgtcta
    27541 atagaccaaa tctgctgtct cgatcaaaat gtagatactc tcgaattaat cgagagtatc
    27601 tgctttaaca ccgtggttct cgatttaaga gctgaaaatg acaattgtgt aactcatatc
    27661 ataattaatc aagaaatggc tcagaagctt tctgagagcc taaaaaaatg ggctgaggga
    27721 ggtaacaatg aaaataatta aaaaaaagat tgctggtagg aaaataaact ggttagctct
    27781 gagcttactt gctttaatgt ttatcgttat tttcaccggt aatgttgctc atgcaagcat
    27841 ttttaattgg ttcgatgata cggaaagtca aactgaattt ttgaataatc ctgtctatca
    27901 accgtattta aaaaaatata acggaaattt ttacagtcaa tttggattgt ggatagggtg
    27961 gtctgttata aaatcgatgt ttgcaattgt agatggtatt caaaatatga ttcctgaagt
    28021 gttagatttg tttagctttg tggaaagtgc tggattgaac aaagtctatc agtcagtttt
    28081 aagcactatt gttgttggct taatgatact ttcactagtg attattggtt ataaaatggt
    28141 gactggaaaa ggaacggttg atctaaaatc tgttggaatc aatgttttaa tgtcgatagt
    28201 attaattctt ttaatgccga ctttgattgg atcaacagaa aaactttcag gaataatgtt
    28261 tgcaaagact ctttataatg acgcaactag tattaacaat caagatggcg gtgtggcatg
    28321 gtcgctgatt gaacaagggg ttactgattt agcttatatc aataaaacgg atcaatattc
    28381 catgatcgat aaaacgaatg atcggaacaa tctaacaaaa gaaaattttt ggaaaacgga
    28441 tcttacgcaa gtattgacag ataatgtaat tgataaaatg gaaaaagaga actcaacagc
    28501 tgataacctg cgttatgaat tagttgaaaa ttctgataat gaatttgttg ctactaaatt
    28561 ttcagataat tttttgtcaa cctttgttga tggtatcaaa tcaggatatt atcgctatca
    28621 agcgaatcta tggggcattt caattgggct gatagcttta gcaatagcct atgtttttag
    28681 cgcctttgtg attattactg caatactcga gttagcattt aaacgtgtac tcggagtttt
    28741 agtatttgct actgacttag aaacaggaca gcgttcaaaa gttgttctat cagatattct
    28801 acagtgctat ttgactgtag gctttcaagg atttggacta tccatgtttg ctatgtttat
    28861 caattttctg aactcaggac aaggtatctc aacaaatatt tttataaaaa caattgctta
    28921 tatctgtgcc gtttttgttt taatcaaagg cagtggaaca gtcatgagat actttggtgt
    28981 tgatattgga ctcaaagaag gttatgggca acttgcttct gcttttggta tgggcgcaat
    29041 gctgtttaga aaaggatcaa atggatttaa tcgtgctaaa ggaagcggaa atggaaatgg
    29101 atcagacagc ggagaaggag aagatcgaaa accagaaaag aatttcggcg aaactctatc
    29161 aaaaaaagct ggcaaaactg gaagagcttt gggatatgcc catgaacgtg gattatcagg
    29221 tttagcttca gatggagcta caatggctag tgaaagagca ataaaaccat ttaagagtat
    29281 gcgtgatatg gctaatgata ctaagaataa gttcaaagaa ggtttagatg atggtaccgt
    29341 gtccgctatt aataaaaata gcaaacctat gttagctaaa aataaagaag atgaaactgg
    29401 aaaatatgct gattccatgc caacacgtct ttcggataga aaagatggtg ctaaggttga
    29461 aaatgccgat agaattatgt ctagttctga acgtatgcgt gaagcaatga aaaataatgc
    29521 tgaatcgaat aataatcctg taagtgcaat tcagcaaaaa gttcaacaag atattgaaga
    29581 acgtaaaaat gctatgcacg gtcaagctaa gtctgctgaa gaattaatta atcagaagcg
    29641 acagggagcg aaatacacgc ctgaagcaat gaatcgtgag gaaatgttaa ggaaacgtgt
    29701 agaagggcgt acaggcgcta atatggacga aaaagaagcg ttgaccaaag aaagaatcca
    29761 agaagctaaa aattcaaata ccggattaga aaaattggtc aaagaaaatg tacaaaattc
    29821 tacaagtagt caaggtggcc gatcagtaga tgtccgagaa aatattcaag gttcattgaa
    29881 tggtcatgtc ggacggaatg ttgatgtaag agagaattta caaagttctt caaatggtca
    29941 aggtactaag acaattgatg ttcgagaaaa tctgcaaagt agcagtacaa gtcaaccaaa
    30001 aacagtagat gttcgagaaa atgtacaaaa ttctacaagt agtcaaggct ttaaatcagt
    30061 ggatgtccga gagaacgtgc aaagtagcag tgcaagtcaa ccaaaaacag tagatgtccg
    30121 agaaaatgta caaagttcta caagtagtca agggttcaaa acagtagatg ttcgggagaa
    30181 cgctcaaaaa gatagtggag aaatgaaaga aaaaacacaa aaaatcaata tcgttgaaga
    30241 cagaaagtca gcgaaaaaat ttgattcaaa tcatgaaaca gtgattattg atagtgaaat
    30301 ccgtgaaaat gataagggat caaaaccaag acgaagattt acctatgaag acaacgagtt
    30361 attcagagat acacttaatg atcctaatcc acttttcgat agtttactta aaaaataaag
    30421 aggtgatttt catgcaatta attctacaga ttagtacaca tgatttctta gaacttgacg
    30481 aattatgtga atcagtaaag aaagcaacaa atgaaaaaat cacggtcact aattatattg
    30541 aactgatgat tcaagaagaa ttaaagatga aaaatgagct tatgaatgaa cttagtcaca
    30601 tacaaaaagg agcatgaaaa atggcaagga aaaaacaaaa atcaattgca gatttgaaaa
    30661 aagaactcgc cgagttggag agaaagcttt atcccgaact gggaaagttt gcctatgaaa
    30721 aaacaggaat ttcatcagta aaagagtttg aagaaacttt tgagattaat caaaaaggta
    30781 ctcgagaaaa atggcaaaag gcggtaaatt tcgcgaatag cgaatttacc ctagtgcatg
    30841 aatttcttgc aagcgatcaa ttgttggaaa cgaagttcca acaatttttg agaagaaaag
    30901 gagagacagc gtatcgaatt ttctatcgag aagagacgca agaagaaagt gttaggtagc
    30961 tgatttatcg actatctaat ctcgataaaa tgcggagcat gagggtgctc gtagggagtt
    31021 tactccctcg acaagcgggg aagggttccc tttcccctcg cttgcttctt ttgaacttaa
    31081 aaataatatt ggaaaggaga agtgcgcatg gcaattgatg tcagtaaact accattagaa
    31141 gaaaggcgaa agcgatataa acgtaatgaa gatagtcgac gacaaaatag gccgataaag
    31201 tttcgagtag acgattatca gtaccaagag ctagaaaaaa cagcagacat ttttggttta
    31261 tctgttccaa tgtacgtgaa gaaaatcgca gtagacaacg cagggaaaag tctaaaatat
    31321 accgatcgac aatataaaga aattaatttt catttactag caataggaag aaatttgaat
    31381 caattagtga aaacagcaaa ttatttgcgt gttcagttaa ataaaaaaga taccgagata
    31441 caaaaagaag aacatcagag tgtttactta tcttttacaa ctaagcaaga gcgcttaaag
    31501 cggctatatg ctaaaaaaaa tgacttaatg atacaaataa aatcaataag tgatccattg
    31561 aatcgcttgg aaaaagagtt gaataagtta tgggctcgat tataaaaatt gcttcaaaaa
    31621 gtaggagtat tcataataca atttcatata tgttaaataa acaagaagat tctgaactcg
    31681 tgacaggaat tggcgtatcg aatgatttaa atgctattga ggattttaaa cgaaccaatg
    31741 aaatttttgg tgataaaggg aaaataaagc tttggcattt tacacaaagc tttgatccac
    31801 tagaagatat ttccgctgaa aaagcacatg agttgggtgt taagtgggca gaaaaaatgt
    31861 ttcctaacca gtcaattttg gtcgtcacac acacagacaa agggcatgtt cacaatcact
    31921 ttggtatttc accaaacgat cagcgaacag gaaagaaaat ggatattcga aagaatgata
    31981 ttcgagtttt gcaggcctgg aatgataata tacttattga taatggaatt agcccacttg
    32041 atatgtataa gtataatcat ttagttgcag atcgtgaaaa ttggaaagaa cgtgaattta
    32101 gaactgaaaa taagatttca aatcgagaaa aaataaaact agctgtttct gaagttgctc
    32161 agtatgcgaa aaattatgat gactttcatg cggaattgaa gaaaaaacat tctgttgaga
    32221 cctatcgatt tgccaaaaat cagcgtattg ggtacaaact cttagattct gaaggtgggt
    32281 taatcttaaa gattggcgga aagcgtttag ggcaaaacta tgatatgaat tatcttgatg
    32341 atttgttcac agaaaataaa aaagcggagc aaattcaatc tgaagaggta ctcactgaaa
    32401 ttgctattcg acatcttggt aaatcgttaa atggtagagc ggatagtaga attaattggc
    32461 aagaacgagc gcaacgcttt gaattagata atgagaatat accatttctt agatcatcaa
    32521 aagattatga agaattttat gacaaatata aatttgaaat agaggacaga atagaaattg
    32581 tattagatgt ttttggtgag aataaagacc aattctttgg atttgcagtg gaagaggagc
    32641 ctttaaagta taagaagaaa tgtgtagatt tggtgtataa aaaagttgtt tatgatatct
    32701 cagagagttt atcaactgat tatgaccaat ttaacgttca tgatactaaa cccgctttag
    32761 aacgtaattt taagaagcgt tatattcaag aaacaaaacg tgttctttat ttatttaatc
    32821 gtgcaattga tgatggtacg attgaaaaag ctcggagaga gtatgaagaa acagcaagag
    32881 aaattgatta tcaaaaacga caaaatcaat atgatatgga tttttgagta aacagggaga
    32941 gagaaaaaat gaactatcca tattttaaag tctcggcttc agaagaaact aaagaaatat
    33001 tcaacaattt ttacaaccaa aacaaaggag tttttggttc caaggcaaat atgtttcgtg
    33061 ttatggtttc aaatttacca gtgttagcta gtccatcaaa taataaattt aatgattctg
    33121 aaagcattaa atttgaacaa aaaatttcag aactagaatc aatgataagt aatgaagtaa
    33181 ttgaaaagct tgatgacatt gatcaaaagt tatcgtattc tctaaaaaac aaatacaaaa
    33241 cggaggagaa aaaagatgtt taaagtttac ttcccgtttc agtggctcgt taaaggaatt
    33301 cttttagtgc tactaggatt gattcttttt ggaagttatc taacaattaa agccagacat
    33361 tttccagaag aaaagccaat aaaaattgta acaatagatt taaaaatagg tgaaacttat
    33421 caggcagatg gggttgaact aacatttaaa aatattgaag tgaccgaaaa aagaaatgaa
    33481 tttgcacaac atgatcgtgt attacaagtg acctatacac ttaaaaatga tacagaaaaa
    33541 ccaatacgtc cacaacaatg gtcgattgaa aataaggatg gtgaagagct ttatgagtat
    33601 gctatgatat tagataatcc agagtatatt gaaagtgaca aagcagaaac aataaattac
    33661 tattatgaat taccgaatga aatgcaggat taccaaatcg tattacgtag agcaaatggt
    33721 tacctagttg ggttgccatt tactttataa gttttatttt gtcagtagct tttaaggtaa
    33781 tatcagtttt gtgatttttg aaaatggaag tgggggaact gggagaatgg ctgaggagaa
    33841 taaagttgtt ccgtttgaaa cattagtgat aaataaggaa gagtttgagc aactttttct
    33901 taattttttt attcaatatg acaggattta tgattttagc tcgtcactag atttatttga
    33961 attagaagca gaaataatag aaaacagtcg agagaagaat ggtaaaatca ttatttcagt
    34021 agaaaaattc aatgaattat tctcagaaac atttgaaata gatccagact attatcaacc
    34081 acaagaagtt tttcgagagt ttagaaatca gattattcgt tcaagtaata ttgaaaattg
    34141 agtcaataaa agaagcgttg aagaaaagtt ccttctttcg acgcttcttt ttaaaagatt
    34201 ttacaaatgt cttatggaga tttgcttcaa tgactacttg tagtcatttc gtttaaaata
    34261 attatgttac acccttatgt gtcacagaga aatactgata taataagaaa taaggtatgt
    34321 aacaatagag tatacttata cggtacatta agaaatgtta agtataaaat taatgacttt
    34381 ttggtctgaa agctgaggga ttcaatggat aaaatattca aaatttttgt gtcatcaaca
    34441 tttactgatt tagaagatgt tcgacttcaa gcaatgcaag gggtattaaa agcgggacac
    34501 atgcctataa tgatggaagg gttcgctgcc acagctacac agaaggaaat tataaaaaaa
    34561 aaaattacta gttgtgatgc gtatatgctt attgttggta gtaagtatgg aacaatcgat
    34621 ccagaatcag ggttgagtta tacagaatgg gagtatgatt atgcattaga taatggtatt
    34681 ccaatataca cgatgatttt agaagaaaaa tatatacagg aacgtatggc aaatgggtta
    34741 cttaaattat cggatataga gactacttct gaaaattaca aagcttttgt gagtaaagct
    34801 agcagtaggc aggttgattt tattaattcc atagagcagg taaaaagtat gacagaggcc
    34861 ggaatcatgc agttggtcaa ggactttgga agctcgatga ttggttgggt tagcgcaacg
    34921 attgttgata atgttaaaat gttagaaaat gaactagata atttgagaaa gacaagaagc
    34981 gagttacaag tgaaattgac aaatgcagaa acaagagaaa taagagagtc agaattaccg
    35041 ccaaatagta gttttgatgg attattatca aaaatgtttg ctcctaactc cactgaccct
    35101 atccttgaac aaggaatgat ttatattatg gagtttattc gagaaaaggt tgaacagctt
    35161 aaagcaacaa ttgagagcca tcgtgggaat attgcattag gatacgatgc taataaaaaa
    35221 gatcaagtac ttttgcgctt aggaaaggat tgcctatttt ttttagctga ttataacaaa
    35281 gctattatca atgtacgatt tgctattcaa gacccttatt tttcggaaaa tattgatact
    35341 ctccatgtta agaatgatgt attgtacagt gagaaatatt cagaaccact ttcactggat
    35401 tacctaaaca aaacgcttag tcaatatcac gaaataaaga aacaacagaa ttaaatttcc
    35461 tgccaaaagc acgccacgca atttgaatac gattttgaat acgattagaa aactaatcaa
    35521 gatgatttat cattaataaa gcgattagct ttaatgaaaa atcattgaaa tatcaactat
    35581 cttcaattat catcaatgaa cttaacttca tggcggaaga agaatagaac aaaagaatcg
    35641 ataaggtgta agaaaagctg gactctctga aaagtagagc ccggtttttt cgtttagcac
    35701 taaatttcct taaaaagtag aaaagctttc gaagcctcgt ttgtttcttt tactgtataa
    35761 taaaagaaaa gagtaacaat cgctaaaaga tataaacaag agaaaggaaa tacaagatga
    35821 ttggtctgat ttattttgtg gtgatcgttc tggcgaacac agttggcgct atttccggga
    35881 tgggtggagg cgtgttgatc aaaccgattc ttgatcttat cggcgcccat tctgttgcag
    35941 ggatttcttt ttactcaaca gttgcggtat tcacgatgtc cattgtttcg actgtgcgac
    36001 aagtatcaag cgggaaatca ctgaattggc agattgtcgg ttgggtatct agtggagctg
    36061 ttgttggagg agtagtcggc aacattgtat tcgaagtttt tttacagtta tttgaaaacg
    36121 agaagcatgt gcagctgatc caaatttttt tgacggtatt gaccttggtt tttgcgttct
    36181 tctacaccaa gcatcatcag ccaaaatttc atttgacgtc atggacttgg taccttatat
    36241 gtgggggggt tctgggcttt ttagctagtt ttttaggtat tggtggtgga ccagtcaatg
    36301 tttctttgtt gatgctgatg tttgcattgc caataaaaga agcaacacta tattctctat
    36361 ctacgatttt cttttctcaa ctggcaaaat tggtaacgat tgctttaaca agtagcttta
    36421 tgcgctttga tttatctatg ttgttttatg ttatccccgc tgctgttatt ggaggtttgt
    36481 ggggagcaag atttagcagg gttttgtctc caaaaaaagt gacctttatt tttcaagcga
    36541 ttgtcatcgt ggttcttttg atcaatcttt acaatgcttt cgtcatcttg caaacattag
    36601 aaggatctgg attcaagcaa gtcaaaatca ctgaaaagtc tttttttaag tgaaaaaagg
    36661 agtatactgt gataaaaaaa ttcatttaaa aaaataaaaa ttggctggtg atttggatgt
    36721 tgcgggaaat gcgtttagga gatgcagaag cactacgaga aatcaatgcg gaacaattag
    36781 gatatgatgt accattaacc gttacggtga gacaaatgaa gaaactgttg aaggagccag
    36841 aaaaaaattt tttcttggtc tatgaagatc ctacttctaa aaaagcagct ggctatgtac
    36901 aagcggagtc ctatgaatcg attttcagcg atccgatgtt caatattatg gcacttgctg
    36961 tgtctaaaca tgctgaaaaa agaggaatcg gaaaggcctt gatgactggc gtagaagaag
    37021 cgaaaagacg aggaatatca gcaataagac ttaattctgc agaatacaga gtagaagcac
    37081 accgatttta tgaacgcatc ggttatcatt ccgataaaat gcaaaaaaga tttttgaaaa
    37141 tcctgtaaat ttttatcaaa aaatacctgg tcatcaagcc gatacttgat gatcaggtat
    37201 tttttttgac taagaaagta attgcatcaa gaaacctgag atgatcaaaa cgatggcacc
    37261 accaagccga ttgcccattt gagcaaaagc aataagttcc atccgattag ctgcagataa
    37321 gacagctaca tttcctgtac cacccatcga gttgttgcat aagccagcag taatagttga
    37381 ttcgattgga tataaaccaa acaaacgacc gacaaatcct gaaactactg aaatcgcgat
    37441 aacacttgtc aaacataaga caacaaattg ccatgtcaaa gcagaagcca atacatttag
    37501 attcaataac gcaataccga taccggcaag aacagctgct gtcaagtttt tgacaatcaa
    37561 gttgttgaac atgatagctg catcttcgta atattctggt aataggtttg cgattttaca
    37621 gataacaacg ataatgatca tgaatgcgta agcatggact ttaggaacaa aatagttaca
    37681 gatttgtcct aagatgaaga aagtcaatga taccatcaaa ccgacaccta attgtggaat
    37741 ggaaggtttt acatttctag gttttccatc gccaaggtcg cctttttcca ttaatttacc
    37801 attaccgttg aattttggca ttgattgtcc cgcacgagca actaacgctg caccaatgat
    37861 agctaggaca tttgtcattg ctgtcgcagg aatcaggcgg gaaatgatag aagaagcgtc
    37921 cgtacctaaa acatttgcat aaatcgttga taaaggtaca gctccggctc caacaccacc
    37981 agccattgcg ggcaatgaga tatataaaac ggaatttgca aaaccatttc cgataagcat
    38041 acctacgagt cctacggcaa aaaaggagac gaccattgaa ataagtgcaa cgggaataaa
    38101 acgaacagaa gctttcatca atagattacg gttcatacca aggatgctac cagtaatcaa
    38161 ggcagcaata taaaagtcta aaaagcccat attgctgaca aagttttctg tggattttac
    38221 tacatactca ggtaagatcc caaatgtagc aatcgcggca gaggcaaaga tcgcaaaaac
    38281 ggagccacca cctaaataac ttttgactat agggattttt gttccgataa aatgaaataa
    38341 attaccgaaa atgaccaaaa cagcgattgg gccaatcata ttcgtcggta attttcctaa
    38401 agccattgca atgatcagga caaggaccat gatcaaataa aggggcaaac ttacgccggt
    38461 aatttttgtt gaccaaaacc cttctttttt cttttcctca actgcagttg cggttgttgt
    38521 tttttgtgcc atttttacag atcctttctt attctgtatc gtatgaaaaa agccaaagag
    38581 aagcggcaaa agagcagtag ttggctggct gctgttttgc cgcagtcgct tttttctttt
    38641 tagacgtcat acaaagtttc tgtatgttct tttactataa caatcttaat cttctaaaga
    38701 tttatattca ggaacccatt tagcagattc aacagcttct ttgatatccg tgatttcttc
    38761 acgattcaat ttttgtgcaa caactgattt tgcgacagtt tcagcaatcg tttgagagaa
    38821 ttcagtgatt tttgctactg gaggcaagat agctgctcct ggttttgtta catcaacgat
    38881 tccgcctaat gctcgactag cttgagaaat gatttcagca tttactcgtg tagctgttga
    38941 agcaatcaaa ccaagcccta aacctgggta catcaa
//
LOCUS       DACDIG010000022        38531 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.022, whole genome shotgun sequence.
ACCESSION   DACDIG010000022 DACDIG010000000
VERSION     DACDIG010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.022"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(180..1154)
                     /locus_tag="IV247_001173"
     CDS             complement(180..1154)
                     /locus_tag="IV247_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAP8777254.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(1135..3018)
                     /locus_tag="IV247_001174"
     CDS             complement(1135..3018)
                     /locus_tag="IV247_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HAP8777255.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDKITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(3036..5261)
                     /locus_tag="IV247_001175"
     CDS             complement(3036..5261)
                     /locus_tag="IV247_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP8777256.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEEKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            5376..6410
                     /locus_tag="IV247_001176"
     CDS             5376..6410
                     /locus_tag="IV247_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8777257.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(6477..7490)
                     /locus_tag="IV247_001177"
     CDS             complement(6477..7490)
                     /locus_tag="IV247_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8777258.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(7568..7756)
                     /locus_tag="IV247_001178"
     CDS             complement(7568..7756)
                     /locus_tag="IV247_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAP8777259.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(8196..8324)
                     /locus_tag="IV247_001179"
     CDS             complement(8196..8324)
                     /locus_tag="IV247_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP8777260.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(8327..9700)
                     /gene="rlmD"
                     /locus_tag="IV247_001180"
     CDS             complement(8327..9700)
                     /gene="rlmD"
                     /locus_tag="IV247_001180"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP8777261.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(9811..10866)
                     /locus_tag="IV247_001181"
     CDS             complement(9811..10866)
                     /locus_tag="IV247_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP8777262.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(10876..12306)
                     /gene="gatB"
                     /locus_tag="IV247_001182"
     CDS             complement(10876..12306)
                     /gene="gatB"
                     /locus_tag="IV247_001182"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAP8777263.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(12303..13775)
                     /gene="gatA"
                     /locus_tag="IV247_001183"
     CDS             complement(12303..13775)
                     /gene="gatA"
                     /locus_tag="IV247_001183"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAP8777264.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(13775..14080)
                     /gene="gatC"
                     /locus_tag="IV247_001184"
     CDS             complement(13775..14080)
                     /gene="gatC"
                     /locus_tag="IV247_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAP8777265.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(14186..16222)
                     /gene="ligA"
                     /locus_tag="IV247_001185"
     CDS             complement(14186..16222)
                     /gene="ligA"
                     /locus_tag="IV247_001185"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAP8777266.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(16239..18473)
                     /gene="pcrA"
                     /locus_tag="IV247_001186"
     CDS             complement(16239..18473)
                     /gene="pcrA"
                     /locus_tag="IV247_001186"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAP8777267.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHHI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            18628..20187
                     /locus_tag="IV247_001187"
     CDS             18628..20187
                     /locus_tag="IV247_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAP8777268.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVVGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(20298..22229)
                     /locus_tag="IV247_001188"
     CDS             complement(20298..22229)
                     /locus_tag="IV247_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP8777269.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(22247..23161)
                     /gene="pfkB"
                     /locus_tag="IV247_001189"
     CDS             complement(22247..23161)
                     /gene="pfkB"
                     /locus_tag="IV247_001189"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP8777270.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(23158..23910)
                     /locus_tag="IV247_001190"
     CDS             complement(23158..23910)
                     /locus_tag="IV247_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8777271.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(24069..24278)
                     /locus_tag="IV247_001191"
     CDS             complement(24069..24278)
                     /locus_tag="IV247_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777272.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(24289..25617)
                     /locus_tag="IV247_001192"
     CDS             complement(24289..25617)
                     /locus_tag="IV247_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777273.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            complement(25982..26569)
                     /locus_tag="IV247_001193"
     CDS             complement(25982..26569)
                     /locus_tag="IV247_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAP8777274.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(26652..27902)
                     /gene="clpX"
                     /locus_tag="IV247_001194"
     CDS             complement(26652..27902)
                     /gene="clpX"
                     /locus_tag="IV247_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAP8777275.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(28149..29438)
                     /locus_tag="IV247_001195"
     CDS             complement(28149..29438)
                     /locus_tag="IV247_001195"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAP8777276.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(29592..30524)
                     /locus_tag="IV247_001196"
     CDS             complement(29592..30524)
                     /locus_tag="IV247_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777277.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHQVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(30681..31340)
                     /locus_tag="IV247_001197"
     CDS             complement(30681..31340)
                     /locus_tag="IV247_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP8777278.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(31343..32368)
                     /gene="sppA"
                     /locus_tag="IV247_001198"
     CDS             complement(31343..32368)
                     /gene="sppA"
                     /locus_tag="IV247_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP8777279.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(32381..32671)
                     /locus_tag="IV247_001199"
     CDS             complement(32381..32671)
                     /locus_tag="IV247_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777280.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            33080..34885
                     /gene="glmS"
                     /locus_tag="IV247_001200"
     CDS             33080..34885
                     /gene="glmS"
                     /locus_tag="IV247_001200"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAP8777281.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(34957..36312)
                     /locus_tag="IV247_001201"
     CDS             complement(34957..36312)
                     /locus_tag="IV247_001201"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAP8777282.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPSDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(36336..37505)
                     /locus_tag="IV247_001202"
     CDS             complement(36336..37505)
                     /locus_tag="IV247_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777283.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(37502..38389)
                     /locus_tag="IV247_001203"
     CDS             complement(37502..38389)
                     /locus_tag="IV247_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAP8777284.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTRAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
BASE COUNT        10475 a         8300 c         6929 g        12827 t
ORIGIN      
        1 ctttttttaa ttttctgata gttaactttg tttttcagaa aaataacaat tggttttatt
       61 gaagaaaata gtcatgaaca aaagtatctg agaacttttt tactctcttg ttcatgacat
      121 atagaataaa gcagctttgt cttgtaaact ccgtttttca aataataatc taaagattgc
      181 tactgaataa taaagccgat ttcatggtcc tcttcgctgc tgattcggta aggtgactca
      241 acatccgctc cccagctatt gatacccccg actcctctta cttttgcaaa aatcgtaaga
      301 accgttcgtc tttctggcgg tagttcttct atatgtgttg cctgttccag ttcttctgct
      361 ttgaatggta aagcggaaaa agcaaatggc gtatcctgct gttcaaatcg caacgaaaaa
      421 tcttcttgat ctttatccgt attatccaac gtttgatttc ttgttagttc tacccatttc
      481 gtttccatat gcatgccgca ttcttgggga acaaggtatg gggaaacggg gagtcctgcc
      541 actttataaa taccttcgac accaccagcc atccgatccg gatatgtttc ccctgatagg
      601 ccttcatact caaaaaattt tgctttcgtt ggcataatga accttaaacc caatgcgggt
      661 aattccggta atcctttttg tccataataa tgcatattga caagcatttt accctctcca
      721 gcgatttggt aggaaattgt cacttgggtt tttggtactg tgttgatttc cagtgtaaaa
      781 atgatttcaa ccgtttcagc gtattcatta cttgaatatt gattatttac tggagcaatc
      841 ggcaaatcga tctttttatt gtccaccagt acttctattt ctttgacttt tggatacaat
      901 ccggctccta accacatacc ggatttaaat ccaaatccgc tacctctgtc attatcggtc
      961 gttgctcgcc aaaaaactgg aagtgtttcc cggtatagcc attcttttcc gtttttattg
     1021 agcgattcca gtccgcctct tgtataagaa aaaatataat gcgtattccc cgatttgaca
     1081 cctaatgtac agtcgccgaa gacgattgca actttttccg tatccatatg acttttattt
     1141 actgtatttt tcataataat atcttacctc ctgcatggcg ggtttttctt gtccatctgc
     1201 aaaaacgatc ccattccctg agaattcata gtcacttggt ttatcgtcaa agtcaccgcc
     1261 atagcgcatc acttctctgc ctgtgattgg atcagtcact tggatcgcct gatctttaaa
     1321 atcccatata tagcctcctt ggtacatttc ataacgatca agcaacgtca tgtaagaatc
     1381 cattccacct aacgaattcc ccatgtcatg catatattca cataagatga acggtttttc
     1441 cgctttattt tcaaggtagt caatgatctc ttctggtggc gcatacatgc agctttttac
     1501 atctgaaatc gaatcttcga atgctggatt tggaaaattc ccttcataat ggacaagtcg
     1561 atcggggtct acttttttga agtaatcgtt catttgtcga atgttttctt ccgcataaga
     1621 ttcatttcca agagaccaaa aaagaatact tggatggttt tttaatgttt cgaaattcgt
     1681 ttttgctcga tcaagaacag ccgcctgcca ctgaggtact gcacctggta cgttgtaaga
     1741 aggctcaaat gcgcccattt tttgccatga gccatgggat tcgaggttcg tttccgccat
     1801 cacatggatt cctgcttggt cgcataaata ataccaagtg atctggttgg ggtaatgaga
     1861 tgtccgtacg ccgttgatct ttgcattttt tatgatagcc aaatcttttt ccatatcttc
     1921 tatagtgatg gcgcgtcctt ttttcggatg ccactcatgt cgatttaccc cgttgataat
     1981 catccgtttt ccattgaagt agatgatttt atctttgatt tcgattttac gaaaaccgaa
     2041 ggaatagtca atatactccg ttactctttt ttcttcgtct atcgtctcca attcaagctt
     2101 atataaattg ggctgaaaat gcccccacag tgccacattt tcaaatccat cgatgcgaat
     2161 cgtcatttgc tcttttaacg gatattgctg ttgatagatt tccttcccat caggatctag
     2221 tatcgctaca ttcagctgac tgtttttggc tacttgctca aaagacaaat ctacttcaag
     2281 tttgccattt tgacatttgt catctaagcg aggtttgatc caaagatctt ctaaatggtt
     2341 ttgtcctcga gcaactaaga agacatctct gaagatccca aaaaatcgga aaaagtcctg
     2401 atcttccaag aatgcagctg tacttctttt gtaaacctcc acagctagaa tattttcttc
     2461 ttccaaataa ggtgttaaat caaattcaga cggagtgaac gaatcttcgg catacccgat
     2521 aaaatgacca ttcaaccaaa taaacattgc ttgttctaca ccagcaaaat gaatcgagat
     2581 ttgttttccc tttagacctt cttctaatcg aaaagtcgtc aaatacgaac caaccggatt
     2641 gtctttagca gctgaaaaga tcccttccgc ctctttctca gggcttgtcg tgaaaggggg
     2701 acggcgaaat acatgtccct cccacggata catcgtattg atgtaatgga tcttgtcata
     2761 atttgctaat tcaatatgac taggaacagt gattttatca aaagaacagg catcaaaatc
     2821 ttttttataa aatgtttttg gtcgtaccga aggatttggc gaataacaaa aacgccatga
     2881 tccatttaaa gactgtttaa gggaggatgt ggtttctact tcttcccttg ttgcataaca
     2941 aacatgatcg ctatgtgcat ttgattggtt cactctaaaa acttgtggat catctaacca
     3001 ttttatattt gctttcatac ttttttcctt ctctctcata actttttgaa atgaagcagt
     3061 tctgtttgga aatcaccttg aaaatcgggg aagtaaagtc caactgtcat caattcggcc
     3121 cccgaaaata tccgcttcga ggtctgttcc tgataaagtg cctcatcatc taaccccatc
     3181 agatatactt catggaacgc tggttgagct gaagctagta tcctccctaa aaacacaata
     3241 gcttcatctt gtttaggaga cacaaacatc catgcggcaa tattttcttc aaaaggactc
     3301 gccaaacgat aataatggcc atactggatc accggacgaa tcgtttgata ggatataatt
     3361 tggtctttta cttgcttttc ttcttcagga gacagctttg tcaaatccag ttcataacca
     3421 aaaacagcgc tcatagctgt ttctcctctt gttttcaacg aagtagatct gtcggtctga
     3481 tgatttggta cagctgatac atgagctgtg atggacgaaa tcggataagc aaggcttgtt
     3541 ccatattgga ttttcatacg ctccactgca tccgtgttat cacttgtcca tgactgcggc
     3601 atataataaa taaacccggc atcaaaccgt ccacctccac cagaacagcc ttcccataag
     3661 atttccggat aatcagttgt caaatgttca agtagttcat aaagcccgag catataacga
     3721 tgcgctactt ctccttgtct ctgtgggtca agggtaatcg aatagacatc tgaaagcgaa
     3781 cggttcatat cccatttgat gtagtcaaga ggaatcgtat ctaaaatctt tctcatttgt
     3841 ttctcaatcg tctgccgaac atctaacctt gtaaaatcca gtacatgttg tgatctggaa
     3901 gcagaaggca ttctacctgg ctcctgcata agaaaatcag gatgtgttcg atacagttca
     3961 gaatcaaccg agatcatttc aggttcaaac cataaaccaa atttcaatcc tttgctatga
     4021 gcataatctg ctatttcacg taatccattt gtcaacttgc cttcatactc gaaccagtct
     4081 cctaatgaag aattgtccgc atctcttttt ccaaaccagc catcatctaa tacgaacatt
     4141 tcgatgccta gctcctttgc ttcatcaatg atcgccttga ttttttcact attgaagtca
     4201 aagtaagtgg cttcccagtt attgaccaga atcggccgtt cagcaaattg atgacgacct
     4261 cttgcgattc tttcacgtag caactcatgg tggatctgac tcattttgtt caatccctga
     4321 cttgagtaag aaaggatcat ttcaggtgtt tgaaaagact ggcctgcatt caattcccac
     4381 gaaaaacgat ggctgttgat cccaccgacg actctcaatt gattgatctg atctttttca
     4441 agttcgaaag aatggttgcc ggaataaaca aattgaagtc cgatcgcttc tccagtattt
     4501 tctgtcgtat gatgatgaac taaagcaata aagttattca tatggtgact gctcgttcct
     4561 cgacggcttt caaatacttt tgttccagat aaaacagatt gccgtgaaat ctgtctttca
     4621 ttgacatgtg ctcctggcaa agcaatgact tcatcaaatc gcctagtatg ggcaaagtct
     4681 agctggaaac tagccgcttt ttctagtttg attgtttctc cggtttcatt tcttatcttt
     4741 acagatcgtg tgatcactgc tctatcagta aatatggtat agaacaaatc gaaataaatc
     4801 gctgtttctc tatccttaag tgaaatcacc aacgtttgtg cctctgactt gtccatcaca
     4861 taagtttgag gcaagccttc gatctctggc ttcccatctg taatataatg gctgtcatat
     4921 ctcagatcca gtaagtctga tccattcttg cgctgaatga tcgatgcggg cgttcgaaag
     4981 tccatgctgc catgagaaga gtattcttgc ggtagtgtat ctttcgacag agaccggtca
     5041 ggatttaagg gaacatttcc tgaaaatcca cgatctcttc ggggataatt tttattgtct
     5101 ttgtactgct tgactggttt gccaaaataa atatgcgcca atacattgct ttcttctatc
     5161 tttatcagat aagaaagaaa tgtatttcgt aaatggaaga ctcggctctt ttcatcaaat
     5221 aagatgaaat ttttatctgt ttttctatgc gtaatatcca tcatttcact cccaacaaaa
     5281 cggaaaccgt ttactttttt ttataattcc tgtatactgt ttactaaaca attttgtcaa
     5341 taaaaatttt ttattttttt gaggagtgtg taaaaatggt tggaattaga gatgtcgcac
     5401 gaaaggcaaa tgtctctcct gctacagttt cccgtgtgtt gaataacgat cagacgatcc
     5461 atgtggctca gactactcgg caacggatag tagaggcagc tgctgcgttg gattatgaca
     5521 tcagtactcg taaatatatc aaaaagagaa tgccttcgat tggtgtgatc tctacgatcg
     5581 gcaaagcgag cgaagaacag gatatctatt acaaacattt gcgagtaggg atggaagaag
     5641 aagctcgacg tctgcattta gggatgaatc gaatctataa tctatctgat aatcctaaac
     5701 aatgggaaga tctagatcag ttaggtgcgg tgatcgttgt tgggactgtt acgaaagaat
     5761 cgattaccgc cttgttaaaa caaaacatgc aaatagtcgt cgtggataac cctgacatcc
     5821 agcaagaggt cgatatggtg tatgcagatc ttgaacggat gactaaaacc gtactggaat
     5881 tgttcttaga aaatggtcat aaaagaatag cctatatcgg gggatataaa gtcgatatgg
     5941 atgaattcgg tgggaagcag atttctataa atgaaaagag gatgcgtgcc tatcagcatt
     6001 ttatgatcga tcatcactta gaaaaatata tgtcttatca tctaggtggc tggacagaaa
     6061 aacaaggtga ggtacttgcg actaatctat tagaaaatga atcccctctt cctactgcca
     6121 ttcttgtcgg gagcgatcca atggcaattg gtgtctatcg ggcattgaaa caaaaaaata
     6181 tccaaattgg agaagagatt gttgtagcaa gctttgataa tatcgagaca atcgcaaacc
     6241 tctcccctgg cttgaccagt gttcaaatca atgcgaaatc aattgggcaa gcagctgttc
     6301 gaatggcatt ggagcgaatt gacggtatac gggacgaacc aatcattttg acttatcctg
     6361 cacgattgat tgttagggaa agttttatcc caagaacaaa aaggtattga aagagaggtt
     6421 gtgaaaagcc gcttcgtttc ttcatactca aaacgaaacg gctttttcac aacctcttac
     6481 ttatacttat tcacaaaatc caatgtctgc tggtaacaat tttgcgattc ttttagcgtg
     6541 taatcaaatt ggtattcatg ggtaatctct ttttttgtat ctgtataaaa caaggattgc
     6601 acgggtacct ttaattctgt caaccgattt tctaatgctt gaccttgctc ctggaatgag
     6661 tatgcatttc cgtcagttat atacgttggc ggaaattcct tagaaacatg gtcagccaca
     6721 ctagcttctt tcaattgctc actcgttttc caatcttttg ttccaatcaa cgaccacgca
     6781 acggttttga cgaaaaactt cataaatcga tcatccgaat gctgctgtgc tgttagcttc
     6841 aaatcaacag gtccgcaata agaaatggtt cctttcagac tatcttttgg aagtagctgt
     6901 ttgattttca tttgtttagc atacgcatca ttcgtttgta tagctgcata ttgcaatgcg
     6961 atttgtgaac ctgcactatc tccaccaaaa aataattgtt ccatgtctag caatttgtcg
     7021 tctttttctt cagctaataa atctttgatc agttcattca cttgaagaac ttgattaggg
     7081 tattctgaat caggcgctaa ttcataattc atcgcaacca ctgcgatgtt ggcgtctgaa
     7141 actaattttg tcgcaaattc tttgacaccc gatttatctc cgccgacgaa tcctccacca
     7201 tgtacccaaa atagaacagg aacaggcccc ttgctgtttt ttggatagta gatgtcataa
     7261 gtattctctt tatacttgga ctcatacgtc aaatctgttt ttttcgccac gtttttttct
     7321 gcttgatcga acgttttttt atcagtgatt tgaacagtat ttgaaaacat atggttgatc
     7381 aagaatgctc caggtctggg ggaaagctgg aaggcaagaa agattgcaaa cgcgatccca
     7441 agaatgattc cgaagaacca tccgataaat ttaagaattt tctttttcat aaggctccct
     7501 cagtagttag tattaagcat tctgattata ccatagtgtt atttcctatc aagctctcct
     7561 agatgattta atgattttcc ccaaacttct tctgcaatgc ttttgacaaa agaaattttt
     7621 tccattgttc ttcaaacaaa acattttctt cttctgttga agcaaatcca cattgaggac
     7681 ttaagcacaa acgatccaaa ggcagatatc gtgtcgcttc gtagatccgg tcaatcactt
     7741 gctggtgctc ttccatttcg ccatttttcg ttacagtcaa aatctaagac aactaattta
     7801 tcttcagaga ctttctctaa aagaacaaaa ctcccagcac gttcactatc gtattctaaa
     7861 taataggcac ggacattttc ttgagtaaaa taatgggtct gctacgtcgt catcccccct
     7921 gatgccgcca aaatcgaata ataatttccg cgtcaaacat gtatatttag tgttgaatca
     7981 tttgagaata tgaaaataaa aatctttttg atcaatcagt taatcaaatt gaaataacga
     8041 aaaatctgag acagaatgac gttcattctg acccagattt gattttgcac aactcaacag
     8101 tccttattgg caaatcgcca aaataatgta gactaactaa aaagactaag aaacaataac
     8161 aaaagttaac acttcttagt ctttctttag cacgtctatt tattaaagat tttagggcct
     8221 tttctcatgt ttttacgatc attcttaggt gtttccattt gtttggatcg gcctccacct
     8281 tgtttctttt tgatcaattc tttcattttt tctttttggt ccatttttat tctcctttaa
     8341 cgaatgaagc gactaattcg atatgatggg tttgcgggaa aagatctaca ggctgaactt
     8401 tcttcacggc ataacctgct tctgcaaata acttagcatc tcttgcaaaa gttgccggat
     8461 tacatgagat atagacgatt cgttctggtg acatttcgat tgctgattca ataaaatgat
     8521 gatctaatcc ttttcgtgga gggtcaacta cgatcacaga tggtttgatc cctttttctt
     8581 gccatttttt cataactttt tctgcttttc cgacttcata atgaacattt tcgattttat
     8641 tatttaaagc attgaactgt gcatcttcaa ttgcttctgg aataacttcc atcccataca
     8701 ccttatccac tctgtctgct aaagacaagc caatcgtacc tattcctgaa taagcgtcga
     8761 tcacaatgtc ttttttatct aactcggcaa actcgatggc tgtttggtac agatactctg
     8821 cttgttgagt atttacttga taaaatgact ttgctgagat tcgataggtt tttcctaata
     8881 gctgatcttc gatatagtct ctgccatata aaactttttc tttgtctcct aaaatcacat
     8941 ttgttttttc ttcattgata ttttggatga ccgaaaccac ttctggcaat tcttcatgaa
     9001 tagcttcagc aatttccttg gatttaaaga atttttcttt acgagtaact aaaacgacca
     9061 tcatttcatg ggaataatga ccacgacgga taatgatgtg acgaagctgt ccttcatttt
     9121 tttcttcatt atatcctcgt acttggaacc gttgcaagat atctctgact actttgattg
     9181 cttgatcgat gacaggatct tggatataaa aatcttcgat agggactaga tcatggctat
     9241 tctttttgta aaaaccggtg gctaactgtc catcgatacg gcgaacaggg atctgtgctt
     9301 tattacggta gccgaatggt tctgccattc cgaccgttgg aagtacagga acttctggca
     9361 tcttagcaat cttcgccatt acatgctcta cttgtttttg tttgaagaca agctgttgtt
     9421 catacttcaa atgggctaaa ggtgcgatac ctatgcgaag taatgcattg ttttctacag
     9481 gctgacgatc aggtgattgt gtaataaatg tttcgacttt tccatatcca aacttattgc
     9541 ctgcttttac tactagtatt tctacttctt ctccagggag tgcattttcg ataaataatg
     9601 gataaccatc gatttttgct acccccattc cttcataact caagtcgacg atttcgactg
     9661 tatatcgttc gtttttcttt actatttgct gttctgtcat attttgctcc tttaagccta
     9721 tttttcaatc ctactgaact ctactataaa tcagctaatt acacaactaa aaccggcact
     9781 gaccttacta cgttcagcac cggcttttga ttaattcttt tgattaggtg tttcgtctgc
     9841 tatttttccg tcaccgtcga tatcttcatc cgttaaacgt tcgacttctt ttacgaattc
     9901 tttgctgaca acctctaatt cttcttcatc tgtacctgtg attgcttcat caggaatctg
     9961 atcggtattg gcaaagaact caatgtgttg atgaagattt tggaaatgca tcggtgcttc
    10021 cccgccgtat tcaccatcta agttgatcat tactggtcga ttgatatcat cttctgtttc
    10081 aacgaacaaa ctgcttgttt tggtatagat tattcttgga tcatcgacat gtcgaccgcc
    10141 attcagcatg agtgctgcaa tatgcaagat ctcaaagata tttgctgtct tcacaatgat
    10201 taaagaaaac ttcccatcat ccaatttggc atctggcgcg atctgttcga atcctccaac
    10261 agagttcgtc agccctagga aaaacatcga agcatttccg atatattccc cttcatcata
    10321 cttcaagcgc attttgattg gctttactcg agggagcatc tctgctcctt tcgcaagata
    10381 agcaagataa ccaaaaatac tctttagttc tgatggaact tcataagtca attctgtcaa
    10441 atagccacct gcagcgatat tgatgaagta actgttatca gaaaggccga tgtccatttt
    10501 tacggtttgg tgttttttta tcacttctgc tgctgcttta atattatctc taggaatttt
    10561 taatgctcga gcatagtcgt tcgttgtacc agctgggatg atggccattt taggacggtg
    10621 ttcaagcgga gcgattccat taaccacttc gttgattgtg ccatcgccac cagctgccac
    10681 tactagatca aaacctagtt ctgcgactct acatgcttca ttcttggctg aatctggttc
    10741 tggtgtagtt gcataggcgc tggcttcaaa tcctgcttct tctaatgcct gtaggatatc
    10801 cgccaaattt tttttcaata attcttttcc cgaggtgggg ttataaatta cgcgtgcttt
    10861 tttcattttc agcctctatc gtttcgctaa ttcatcttta agtagcttat tcacgacacc
    10921 aggatttgct tgtcctttag ttgctttcat gatttgaccg actaggaagc caaccgcacg
    10981 atcttttcca tttttgaaat catcgacaga ttgttggttg ttatccaaaa tttcattaat
    11041 gattggcagt agtttgttcg gatcagataa ttgcacccaa ccattttttt caactacttc
    11101 ttgggcttct ccgccatttg tgatcaattc acggaagact tttttggcga tttttgagct
    11161 gatcgttcca tcggcgatca aacgaatcat tcctgctaag ttctcaggtg tcaattttgt
    11221 ctcagctaat tccagtttct cactgttcaa gtaagcagaa acttcaccca tcaaccagtt
    11281 agatgcttgc ttggcatctg caccattttc caaaacagct tcaaaaaaat cagacatttc
    11341 ttttgtaagt gtcaatacca ttgcgtcata ctctggaagt ccaaactcac ggatatagcg
    11401 ttcgcgacga gaagctggca tctcaggcaa actagctttt actttttgaa tccattcgtc
    11461 atcgatcacg aatttcggga tatcaggctc tgggaagtaa cgataatcac tagacccttc
    11521 tttgacacgc atcaaaactg ttttattcgt tgtttcgtca aaacggcgtg tttcttgttg
    11581 gatttctcct cctgaaagaa gaacttttgc ttgacgtttt tcttcaaaag ctaaaccttt
    11641 tttcacgaaa gataaagagt tcaagttttt caattctgct ttcgtaccga actcttcttg
    11701 tccataaggg cgaagagaaa tattggcatc acagcgcata gagccttctt ccattttcac
    11761 atcgctgact tctgtaaatt ggataattga tcgcaatgct tctagataag cgtatgcttc
    11821 ttctggtgaa cgcatatcag cttcagaaac gatttcgatc aaaggcgtgc cttgtcggtt
    11881 caaatcaacg taagaataac cgccaatacc atgcatgttt tttccagcat cttcttctaa
    11941 atggacacgt tcgatccgga tttttttctt tttaccttct acttcgattt cgatccatcc
    12001 gtcatgaccg attggttgat caaattggga aatttgatat gcttttggat tatctgggta
    12061 aaagtaattt ttccggtcaa aatgagtatc tttagagatt tcacagttta atgccaaagc
    12121 tgctttcatc ccgaattcca gcgcagcttt attcattact ggcagcacgc ctggataacc
    12181 ccagtcgatc acgtttgtat tgctgtttgg ttctgcacca aaatgagctg gcgctggaga
    12241 gaaaattttt gagttggttt ttaactcgac gtggacttct agtccaatga ctgtttcaaa
    12301 attcattcgt ctttccctcc taaaatagct ggttgttgtt tatggaattc tgttgcttgt
    12361 tcaaacgcat atgccgcttg atacatcgtt ttttcatcaa aataattacc gatgatctgc
    12421 aatccaactg gtaatccttc tgataaacct gccggtacag acatacctgg aagtccagcc
    12481 aagttgacag ggatcgttaa gatgtcactc atgtacattg tgattggatc gttaatgttc
    12541 tcaccgattc cgaatgcaac tgttggtgaa gatggcccga tgatcaagtc ataatctgca
    12601 aagacttttt caaaatctcg tttgatcaat gtacggactt gtcctgcttt tttgaagtaa
    12661 gcgtcataat aacccgcgct taaggagaat gtacctaaca tgatacggcg ttttacttct
    12721 tcaccgaatc cttctgaacg tgagttgaca tatacatcat ctaatgattt cacattttca
    12781 gagcgataac cgtagcggat accatcgaat cgttgcaaat tggaacttgc ttctgatgag
    12841 gcaatgatat aataaacagc cacaccgtat tttgaatgag gcaagctgac ttcttccaca
    12901 acagcaccta atgaacggaa tgtttctgct gctttcaata cagcttcttt tacgccaggg
    12961 gccacacctt cgcctaagta ttctttaggt aaagcaatct tcattccttt gatgtcgcct
    13021 gtcagtccct ctgcaaaatc aggaactgaa acgcctgaag aagtaccgtc tttttcgtca
    13081 taaccgctga tagctgtcaa tgctaatgca ttatctttca ccgtacgggt cattggtccg
    13141 atttggtcaa gagatgaagc aaaggcaatc aaaccaaaac gtgagacacg gccataagta
    13201 ggtttcatcc ctacgatgcc gttaaaagct gctggttggc ggatacttcc cccagtatca
    13261 ctacctaaag aaactggaat ttgtccagct gcaactgctg ctgcagaacc accagaagag
    13321 ccaccaggta cttttgttgg gtcccaagca ttttttgttt tcttgaaata agaagtttct
    13381 gtacttccac ccatagcaaa ttcatccatg tttagcttac caacagggat catatctgct
    13441 tgatagattt tatccatgac cgttgcatca taaattggtt caaaattgta aaggatctta
    13501 gatgcagcgg ttgtcaagat atcttttgtt acgatattat ccttgatacc aattggaata
    13561 ccagcaagga cgttgctttc atcaatccct ttttcatcta aagcttttgc ctgttccaat
    13621 gctttttctt cgttcaatgt aatgaatgcc tcgacctcag gctctgtttc tttgattcgt
    13681 tcaaatgttg tttttgtcaa atcttgtaca gtgatttctt tcgatacaag catatgatgc
    13741 agttcttcta gagaacgatt gtgtaattct gtcattatgc gccagcctcc ccattatcga
    13801 tgattgcggg tactttgata aagccgtctt ctgtttccgg cacatttttc aataattcat
    13861 cgcggttcca tccagcttgc gcccgatctt gtcgcatcac gtttactgtt tcaaccacat
    13921 tcgacgtaaa tggaacacct tccgtatcta cttcttccag catttcaacc atatcgatga
    13981 ttttacctaa ttggtcggta aaacttgcaa gttcttcatc tgcgaacgca agttttgcta
    14041 atttggcaac gtgtttcact tgttcttcac taatcgccat actacccctt ctttctaaac
    14101 gagttaaaaa ctttgctctc tattttaaca caagagtgcc gtgtctgaaa ttgtttattg
    14161 aaatttgtct gaaaaaactc atttttcatt cttctactga gcgactttta gccaatgcat
    14221 ctgccatttg atcttctgtc caaacttcga ttcctaattc ttgtgctttg gtcagcttgc
    14281 tgccagcatc ttctcctgct accacgatat ccgttttttt cgatacgcta ccagtcactt
    14341 tcccgccaag attttcgatc gtctcttttg cttcttcacg agtaaatcgt gttagtttac
    14401 cagtaaggac tactgttttt tctttaaaag gagactcgac ttcttgcaat tgggcatttc
    14461 gaagcccttt gtattcgaaa ttgacgcctg cttgttttaa ttcattcatc aattcatgga
    14521 cttcttcgtt actgaaataa gtgactacac tgtcggcaat gatatcaccg atcgtatcaa
    14581 gcgcaataat cgactcatag tcacttttac tcaatgtttc tagatcaccg aaatgttctg
    14641 ctaaaatttt tgctgctttc gctccgacat gtcggattcc taacccgaaa atcaaccgct
    14701 cgaccgaatt atctttgctg ttgtcgattg ctgtcaaaat attatttgct gatttttctt
    14761 ttattttgtc caacgtcaat aactcttctt ctgtcaattt gtaaagatca gcaacatctg
    14821 ctactagttt tttatcatac atctgttcta gtacgcgcgg ccccaaccca tcgatattca
    14881 tcgcatttct tgaaacaaag tggttcaaac cttctttcat ttgagcaggg catttaggat
    14941 tgatgcaccg taaggctact tcttcatcca aatggacaag ttcacttcca caaactggac
    15001 aatgtgtagg caattggtat tcttcgctgt ctttaggtcg tttatctaac acgacttgcg
    15061 aaacttctgg gatgatgtct cccgctttat aaatcagaac tgtatctttc aagcggatat
    15121 ccttcaactt aatataatca ccgttatgca ggctggcacg gcttactgtc gttcctgcta
    15181 ctcggactgg tgtcatgata gctgttggcg ttactacccc tgttcgaccg actgtccatt
    15241 cgatgttttc gattagcgtt tcaacttctt ctggcggaaa cttgtaagct gctgcccatc
    15301 tcggtgcttt gatagtaaaa ccaagttgat cttgtagcga gaattcattc actttgatga
    15361 cgattccatc gatttcatag ggcaaatcca cacgtttgtc atgatattct tcaatgtatg
    15421 accacacttc atcaatcgaa tgacatagac gtttttcccg attcgtgtgg aatccaattt
    15481 tttctagctc ttctagcgca tcatactggg tttttgcttg cataggaccg aaatcagcta
    15541 ctgtgtataa aaaagtgtcc agatttcttt tcgctgtgat tttagaatct aactgacgca
    15601 aagaaccagc tgcggcgttc cgaggattcg caaagatatc ttttccctct gcttctctgt
    15661 cttggttcaa ttggacaaat gaccgttttg gcatgaaaca ttcgcctctt acttcgatgt
    15721 tcattggttc ttttaatttg attgggaccg accggacagt ttttaggttt tcagtgatgt
    15781 tttctcctac agttccatcc ccgcgagtcg cacctctcac gaaatttcca tcttcataac
    15841 gcaaagaaac ggaaagtcca tctattttta gttcacagca atagtcaact acacgtccca
    15901 ctgctttttg tacacgttga tcaaaagcta tcagctcttc tttgctgaaa acgtcgttca
    15961 aactgtaaag cggaatatca tgagtgactt tttcaaaacc ttctaatact tttccgccta
    16021 ctcgttgagt gggagaatca gacgtgatca aatcaggata ttgttcctcg attgctacta
    16081 attctgcgta ttctttatca taaacatagt cttccactgt tggtttatct tctacgtagt
    16141 attcgcgaga ccattggttc agtcttgtac gaagttcttc tgctcgttta gcagcttcgt
    16201 caatcgtcat ttcatgttcc atcatttacc ctccactact aagctttttc aattggcgca
    16261 aaggcagcca aaagacgttt tactccttga cttgggaaag ccacatctag ttctagatcc
    16321 ttcgacgtcc cgcttactcg gacgactgtt cctagacccc attttttgtg tttgacttta
    16381 tctccaggtt tccagctgtt attttcccct ccagtagcac ttttagaggg aacaaccgct
    16441 gtctctggct gtttatagct atatgctgtt tttttagcga atacaggcgt ttttttaggc
    16501 gtaggacgca tgccttcgat ttccagtaac tcctgatcga tttcttctac aaaacgagaa
    16561 ggtcggttat attgcgtccg tccatataat gttctggaga aagcatttgt taaatacaac
    16621 gcttcttctg ctcgcgtgat ccccacatat gcgagacggc gttcttcctc cagttcgctt
    16681 tcttccatca atgctcgaga taaaggaaaa accccctctt ctagaccaat caggaataca
    16741 actggaaatt ctaatccttt ggctgcatgc aaagtcatca atgtcacttg ggaagcatct
    16801 tcttctaaat tgtcaatgtc ggaaacaagt gccagatcat ttaagaagac cgttagtttt
    16861 tcttctggtg catcagcgtc ttcttcattt tgctgttcga attgtttgtc gaattcttgt
    16921 gtgaccgtta aaaattcctc caagttttcg atgcgtgctt gtgcttctaa tgtattttgg
    16981 atttttaagt cttctagata tccgctacga tccaaaacct ctttcgtcaa ttctgtcact
    17041 gtcaagtatg ggatcatctg agtgacttct tggatcatct ctccaaatgc acctaattgc
    17101 tgtcctgctt ttccaccgat atttgccaga tccacatttt gcgctgcttc aagcagtggc
    17161 cattcatgaa gagaagcaaa actgcgtagt ttttcgatag atcctggtcc gatgcctcgt
    17221 ttgggcgaat taacgatacg ctcaaaactg attgaatctt gaggatttac cagcacattc
    17281 aaatacgcta aaatatcttt gatttcttta cgatcgtaga acttgtgccc tccaaccatt
    17341 ttataaggaa tatttgcttt caacagagtt tcttccatta cacgagattg tgcgttcgtc
    17401 cggtacaaga tggcaaagtc gccgtaattc cgatgattgc ttcgaatctc ttcttgcatc
    17461 cggtctacga taaaacgggt ttcgtctcgt tcattatctg cacggtagta agtgatcttg
    17521 tttccttctt tgttttctgt ccacaagttt tttggttttc gattgctatt gttttcaatg
    17581 acttggtttg ctgcgctaag gatgtttttc gtggagcgat agttttgttc tagcaaaatt
    17641 actgcagcat ctggataatc tttttcaaaa tccaagatgt tctgcatatc tgcaccgcgc
    17701 cagccgtaaa tactttgatc tgcatctccc acgacgcaca ggttcctaaa tctacctgcc
    17761 aataaattga ctagcgtata ctgtgcgtga tttgtatctt ggtactcatc tacatgaata
    17821 taatggaatt tgttttgata atacgtgagt gaatcaggat gttcttcaaa taatcgaatc
    17881 gtattcatga tcaggtcgtc aaagtccata cattgattat attgaagctc tttttggtaa
    17941 gcagcataac attttgccgc aatctcttcg taataactgc cttgcatttt tgcaaaatcc
    18001 tgaggggtct gcaggctgtt ttttgcttga gagattgttc ctaatatact tcgaggatca
    18061 tattttttcg gatcaatgtt cagttctttt agtatccgtt tcatcaaggt cagctgttca
    18121 gaagaatcga tgattgtaaa gttccggtta tacccgataa aatcaacatc tcttcttaaa
    18181 atacgcacac acatcgagtg aaaagtcgat acccagacat cttcgcctcc agatgcgaga
    18241 atcgcattga cacgttcttt catttctttt gcagccttat tggtaaaagt aatcgctagg
    18301 atgttccaag gattgacttc tttttcctca atcaaatagg caatgtggtg tgtcagcact
    18361 ctcgtctttc cacttcctgc acctgccatt agcagtaatg gtccgtcagt gtgaagaaca
    18421 gcttctttct gacgggggtt catcccgttt aataattcag ctttggatga cattaacaac
    18481 atcctttctt ttatcaatct tgttaattat agcattttct tggctttcat taaactagct
    18541 agttcaaaat catttgtttc tttttatgga tttttccttg tcaattcgtt gatttcgcgc
    18601 taaattaaaa gaaagcgagg cgtttgaatg gaaaaaaaag gaaaaatcaa gaaattagat
    18661 aagactcaga tatattttat tctaaaagga atatttgttg gactgctgac aggaatcgtc
    18721 gttagcctgt ttcgattatc tatcgaaaag ctaagcgatt tagttcgaag tatctatgaa
    18781 atgagtcggg aacagccgat ttacttaatt ggcctagcta tttgctgcat ccttgcagca
    18841 tttttcgtgg gttatctggt caaaaatgaa ccggatatca agggaagcgg gattccacaa
    18901 gtagtgggac agcttcgcgg ggaattatca atgaactggt tctctgtttt atggaaaaag
    18961 ttcattggcg gtgtactttc agttggtgcg ggcctttttc taggtagaga aggaccttct
    19021 atacagcttg gcgcatccat tggacaagga gctggacaat tgtttcggag tccatcttca
    19081 gaagaaaaga ttttaatttc tagcggtgca agtagcggac ttgcagcagc attcaatgct
    19141 cctatcgcag gattactctt tgttttagaa gaaatccatc actccttttc tccacttgtt
    19201 tggctgacat catttgcttc agcaatcact gctaacttcg tctctcttta ctttttcggc
    19261 ttgagacctg ttttatatct aggtgacctt ccttctttgc cgttgaaata ttatggttcc
    19321 ttagtgatct taggagtact tctaggcatc ttaggtttta tttatcagaa tgtcttgctg
    19381 gctttgccaa aattttacaa ccgattgcct ttaccagctt attttcacgg gttcgttcct
    19441 ttcttgctga ttttgccaat tggttattta tggcctagta ctctaggcgg aggcaatcaa
    19501 atcgttttag cttttggaca tgcttccttg ccaatcatta tgattatcgg tttgtttgtt
    19561 cttcgattcg ttttttccat gatttcttac ggtgggggtc ttccaggggg gattttcctt
    19621 ccgatattga cattgggggc tttaatcggt agcttttatg gaaacattat ggttgatttc
    19681 gggatggatc caatctacat caaggatttt gtcgttattg ccatggctgg ttatttcaca
    19741 gcgattggaa aagcaccgct gacagcaatt attttagtga cagaaatggt tggctctctt
    19801 aatcatctga tgcctttagg attggctgct cttgttgctt atatcgtcaa tgaccttctc
    19861 ggcggtaatc caatttacga atcattattg gaacgtctac ttggaaatac tcagaccgcc
    19921 tcactaaaag gtgcaaggac aacttttgat tttccagtaa cggcagagag ttctttagac
    19981 ggattaatgg tacgagactt caattggcct aaaaatatgc tgctaatctc tatacgcaga
    20041 ggaaatcagg aaatcttgac tcacggtgat acgatcatga atgtaggaga tattttggtt
    20101 atcttgactg atgaagcaaa cttgccaaga atcaagcagg aaatcgaggc taaatcttct
    20161 atcacaaatt tgaagcaatc attttaacga acatggaaaa actattttac aacaactatt
    20221 catctacact acttgattct atatgattct ataaaaaaag aagaggctgg gaaaacccag
    20281 cctcttcttt tttatttcta tgcttctgct actgcaggag cttttctcaa taaaccataa
    20341 atcacaccag atacgactgc tccgatagca ataaacacca aatacatgac cgggtgactc
    20401 agtaagaata caacgaaaat cccgccgtga ggagccagta atttgattcc gaaagctccg
    20461 actaatccgc cagttaaagc agaaccaata ataaaacttg gaatggcacg aatcgggtca
    20521 gctgctgcaa atggaatcga tccttccgtg acaaacgata gtcccatcac gatgttcgtc
    20581 aaaccagcat cttgatctgt tttgctgaat ttattcttaa acaaacgtgt agcaacgaaa
    20641 attgccaatg gaggtaccat tcctccagcc ataacagaag ccatcacgac acttccgcca
    20701 gttgcaacag tcgtagctaa agttgctgta ccaaacacat aagcggcttt gttgattgga
    20761 ccgcctaaat ctgccgccat cattccagct aataatgcac caagtaaagc agcattcgaa
    20821 ccactcatac ttgtcaagaa accgttcaat gcatcattga ttgctttcat agggacattg
    20881 accagcaaca tcaagaaacc agtaatcagt aagccaaaca ctggataaaa gaggatcgtt
    20941 ttaattccat ccaatgattt tggtaagttc ttgaataatt tacgtaaaaa tacgatgaca
    21001 taaccagcta agaatccacc aatcagtgca cctaagaatc ctgcaccgcc tgtattagct
    21061 aatgcaccag cagcaaatcc aacgatcaaa ccagggcggt caccgatact tgaagcaata
    21121 aatcctgcta ataccggcag catgaaacca aatgcagctt gtccgatttg gttgaaccag
    21181 ctagcagctt gattgtaatt ccctagattt gataattgat catgtggcac gccaataaat
    21241 tggtcgatca tgaaagaaag cgcaatagcg attcctccac caataacgaa aggtaacatg
    21301 tgtgatacgc cgttcatcaa atctttgtaa attctttgtc cgatagtacc atccgcattt
    21361 ccttcttctg aactgctttc ttttccatca ccatggaata ctggtgcagt accactaagg
    21421 gctagattga tcaactcttc tggtttacgg ataccatcac tgacaggacg attgatcagt
    21481 tcttttccgt caaagcgatt catctctact tttttgtctg ctgcaacgat tacaccttct
    21541 gcacgggcga tgtcttcagc agtcaatcgg tttttgattc cttctgaacc gttcgtttcg
    21601 actttgatct ccacacccat ttctttcgct tttttcttca aagcatcttc tgccatgtaa
    21661 gtgtgagcga tccctgttgg acaagctgta acggcaacaa cgaattttct ttctggatga
    21721 gcagaagctt ctgcagcttg tgtttcttgt tctgcttttt cttctgcttc tttttgctct
    21781 tcagctgttt ggaacaagtc ttggacttct tctggtgtat ttgcttcttt taattttcct
    21841 acgaaatctg catcaatcag caatcgagat agtgctgcta atgcttgcaa atgtgtgtcg
    21901 tttgcgcctt ctggagctgc gatcatgaaa aacagataag ttggctgtcc gtctaaggct
    21961 tcataatcta ctcctttttg gctttttgcg aataaaactg ttgcttcttt aactgcactg
    22021 tttttcgcgt gaggcatggc gattccatca ccaagacctg tagaagtttg tgcttcgcgg
    22081 gctagaattc cttctttata tgtttcgata tcggaaatcc ggccggcatc aaacattttt
    22141 tggaccattt catcgattgc gcctttttta tcagtcgctt gcaaatccat gatcatcgct
    22201 tctttgacca ttaaatcctt gatattcatg attatcctcc ttgaaactaa tcaatttttg
    22261 tgatttcaac ttctggcagc aactcatcaa tcaattcgcg agctgccaag tcatcagaaa
    22321 aagctgttgc gcttccgcaa gcaactcccc atttaaaggc ttcgattgga tctttcgtac
    22381 ggctgtaagc cccaataaag ccagcaatca ttgaatcccc tgcacctact gaatttttca
    22441 atgggcgaac taatacattc gttttataag ttccttgttc cgtgaagagc aaagcaccgt
    22501 ctccagccat tgagatcagc acattctgtg ccccttcagc tagtaatttt ttcccatacg
    22561 gaatgatttc ttctacagat tggaaggttg tgttgtaaag ctcagctaat tcgtgattgt
    22621 ttggtttgat caacaacggt ttcttaacaa gtgcattttt taggtcatca cctgttgtat
    22681 caatcacgaa atctgcacct ttttctcgaa tgatatcaat cagttcttga taaaatcctt
    22741 ttcgcaggct agctggcgcg cttcctgaca atacgacgat atctccttgt cctactgttc
    22801 ctaatgcttg cttcaactca tcgatttctt catctgaaat agatgggcct aagccattga
    22861 tctcagtttc agtgtctgat tttagtttga tattgattct tgtatcttct tttacggttg
    22921 tgaacaaaga ctggatttgt tctttttcaa gccacttcga aataaattcg ccagtgaacc
    22981 ctcctaaaaa ccctagtgct gtagattgag aatcgatccg tttcaaaata cgggaaacat
    23041 tgatcccttt tcctccaggt aacttgaagt cttccttcat tcggttcaaa tcgcctagtt
    23101 ttaacccatc tacgcgtacg atataatcaa ttgatggatt aagtgttact gtgtaaatca
    23161 ttgtattgcc tccttaattg ttgtcttttc acggaattca ttaacgaatt ctaatggaca
    23221 acgctcggtt atgatcgtgg ctgcttccaa aggtgcaaca gaggtgaagg acaactggtt
    23281 gaatttggta tgatcaatca atacaaatgt ctcttcggaa tttttcatgg ctaaacgttt
    23341 aacagctgcc tcttctggat ccggagtcgt gaatccttgt tcaagatggg caccgttcat
    23401 tcccataaat gctttattaa aatgaaaatt ttttagttgt tctatcgcat tagatcctaa
    23461 aacagcatta gttgccagct ttatcgttcc acctaaaata atggtagcaa gctccaaatc
    23521 aactaaattt gccgcatgtt gaacggaatt tgtcactact gtgatctgtt ttccttgcag
    23581 aaaaggaatc atttctaacg tagtagagcc agcgtctaaa taaatgacat cttcgtcatt
    23641 aatggtttca gctgccgatt tggcgatttg ctgtttttcg tgaacgtttt tgagtgattt
    23701 ttcactcata ttctgctcaa aattcaataa ttgctctttc ttagctccgc catgaactct
    23761 ttctagaaac ccactattct ccagctcttg taaatcacgt cttatcgttg attcagaagc
    23821 attcaaaagg tttacaagat cttgtgattt cacgattttc tgctgttcaa gcaagtctag
    23881 gattttttgt ctgcgttcct ctgtaagcat tttcacgatc acctcaatac aaataatagc
    23941 atattttttt tctactagca atataaatca gtcaaaaacg ttcataaaaa ttataaatca
    24001 gtcaaaaaaa aataaaaaac cagtcagaaa gactggtttt ttattttttt gtctatcatc
    24061 aagccgtttt atcgtttttt attttcgctt cttgtttttt tcatataaac agaagcaggt
    24121 tttctttcct tatgaagaag ataataataa agtcccatcc cgccaaacat gaccaaaaaa
    24181 ccgatgactg atcgaatagg aaaattagga acaaaaaaga gacagacgga taataatgat
    24241 gccggaacca ttattcgata atagagttgt ttatccattt tcttcacctc atattttttg
    24301 taaagtaatc aattgttcta attctcggta atctaaaaat tgcacacgca gatgctgctc
    24361 acctttcttg acatcgaatt ggatacttcg ccgctttttc tttattcgtt tgaaggtaac
    24421 atcagagagt ctatgtttga tttcctttaa cggtaattct gacagggatt ctgaaggaac
    24481 atagattctt aaggataaaa gatactcttg atatttttca tctgttacct ctacttctag
    24541 atattcccta atataataac aataagcttc gattccatta tccaaagcaa cagtcggatc
    24601 gataacttta tagcacatca tgcgtgatcg attctcgtca acccaaggaa aatacgcctg
    24661 agcatttagt gttccgcgaa cacgcctttc tttataatct ccttgttgga tatcataact
    24721 ttttgttaca aaaaatgaag cattcttttt aggaacaggt tgcatttgtg cattctgata
    24781 ctggacagat tggatcgtat ggtcagtgta aatgtattca acgaatggcg gtttcaataa
    24841 accgacacca ttgtcccaag caagatgatc aaacccaagc tggtccctaa aataggattg
    24901 aaacatttga ttgtttggct tttgccagtt aaaggggtct cgataaagct ggatacatat
    24961 gacttcgttt tctaattcct tcactttttc tacttgttgg atgtcgagaa aaatcggttt
    25021 agggatgatt tgatgataat agcgactata atacatttcc caacacgaag tgaaacataa
    25081 accttggtaa aaaagatcgt atcctggaaa ttggttgcaa tcgactacta cttccccgtt
    25141 ttgttgtttc attttaggta aatgttcgat ttcatccttt ttctcaaaca atagccgttg
    25201 agagatctgt tttgtattgt gatagataaa ctcgctataa gctcgcatat aggcgaacac
    25261 cccatgagca atcatttttt tctgaatgaa gtctcgaatc aaaaattgct tttctttgaa
    25321 aacagagata gacaatacat tttttatcaa taattgatta tcttccaagc gaaaactaca
    25381 ttcgttctcg tcatttccta caactaacca actttcgtct cttaccgatg ctagcattat
    25441 tttttcttga agctttcgaa gatcatacgg atccgcttgt tgtgtgaaag agcggataaa
    25501 tgttggctgg atcaacagac gtgtcaaaat agcccaatcg cttatttcct gttttttttc
    25561 ttcttcattt aagtctgtga gctggatagt catcaataat ttatcttcgc caatcatttt
    25621 ctcacctctt tcaaatcttt ttataattta attataacca atgataaaga aaaactaaaa
    25681 ctttatgtct tagaaaccaa tttggacgat tacatgatag actaagtaaa aagaaacaaa
    25741 gaggtgttta caatggggtt gaaatttgaa cgtacagata atttagataa acttttcgaa
    25801 tcatttgctg tcgatccgga aaaaacagtt gcaccaaaag aagagatcga tcgatttcta
    25861 aaaccacagc cacaaaatga aaagaaaaaa gacaagaaac aaaagtaagt taagctcaaa
    25921 aaaagactta ccagccaaac ttttatgaaa gagtttgact ggtaagtctt ttttattctt
    25981 tttatgattc aattgcttgt tcgattgctg cccacgcttc ttcttttccc tgttttgtaa
    26041 cggaagaaaa gagaataaac gtgtcattgc gatcaaaatc caacgctttt ttgatttcgc
    26101 tctcatgctt gttccattta ccacgaggga tcttatctgc cttagtggca acaacaatta
    26161 cagggatatc ataatacttc aaaaattgat acatctggat atcttcttga gaaggcttat
    26221 gacgcaaatc tactaaagaa acgaccgcac gtagctggtc tctagtcgtt aaatatgtct
    26281 cgatcatctt tccccatttt gctcgctcag ttttggatac ttttgcatac ccatatcccg
    26341 gaacatcgac aaaatgtaat gcgttctcga tcaaataaaa attcaatgtc tgggtctttc
    26401 ctggcttacc ggaagtacgg gctaaattct ttcgatcaat caatgtgttg ataaaagaag
    26461 atttccctac atttgatcgt cctgccagag ctatttctgg caattgtgta tctggatatt
    26521 gttttggtgc cactgcactt atgataattt cagcttgatg gactttcatg gtctctctcc
    26581 ttaacaaggt gatgaataaa gcaagcggct gtcgcaaaag ccaaaatgac ttttacggca
    26641 gcctcttttc atcaacccgc ttttttctct gaatctttat agattactgt tggctcacca
    26701 gacaattcag ctgcttcttt tgtgataatc actttttcga tgttttcatt tgaagggata
    26761 tcaaacatca catccatcat gatttcttca atgatggaac gaagtcctcg agcaccggta
    26821 ttgcgttcga ttgctttttt cgcaatggct ctcaacgctt caggttcgaa ttcaagttcg
    26881 gtattatcta gagaaagaag tttttgatat tgtttgacta gcgcattttt cggctcagtc
    26941 aagatccgta ccagatcatc tgttgtcaat ttctctaaag ctgccataac aggtaatcgt
    27001 ccgataaact cagggatcaa tccgaatttt agtaaatctt caggaatgat atgctgcatg
    27061 acactttcgt cttcagaaag tttttggttt tgtgtaccaa atccaatcgt cttttcgccc
    27121 atacgattct tcacaatcgt ttcgatgcca tcaaatgcac cacctacgat gaacaatatg
    27181 ttcgtcgtat caatctgaat aaattcttga tgtggatgtt tacgtccgcc ctgaggtggc
    27241 acacttgcaa cagttccttc tagaattttc agcaaggctt gttggacacc ttcgccggaa
    27301 acgtcacgag taatagatac attttcgctt ttacgcgcaa ttttatcgat ctcatcaata
    27361 tagatgatcc ctttttctgc aagttccaca ttgtaatcag cagattgaag caatttaagc
    27421 aaaatgtttt ctacatcttc ccccacataa ccagcttctg tcaaacttgt cgcatctgcg
    27481 attgcaaaag gtacattcaa tgtttttgct aatgtttgtg ctaaaaatgt tttaccagac
    27541 cctgtaggtc cgatcaagca gatattgctt ttttgcagtt caacatcatc tgtgttttct
    27601 tgtgcttgct gattgacacg tttatagtga ttgtatacgg caacagctaa cgagcgtttt
    27661 gcgcgatctt gtccaatcac gtagttattc aatacttcaa gaatttctag tggtttaggg
    27721 acttcagtga attcgcgtac agcttcttca taaaattctt catcaatgat ttctttacat
    27781 aggtcgatac attcattaca gatgtagacc cctggaccag ctacgatctt tttcacttct
    27841 tcttgggttt tcccgcaaaa agaacagcgt acggtctcat tgctacttgg attgtcatac
    27901 atggtcttca ccccttaact tgcaaaaagt atgcaagaaa ctatcaaaga ctttacagtc
    27961 ttttactatc ataacatagt ttagtaaaaa gaaaaagtgt ttaacaaagg atcaactgac
    28021 ctataccttt gcatttttta ctcttaacaa aaaataagga actgtgacaa aatcgtgtca
    28081 cagtccctta tttcgaataa acaattttct agaagtagct tcttcagaaa tgatttatta
    28141 cttgatttct attctttcgc ttctgtcgct gtacctgtga tcaattcgat tgcttttttc
    28201 attgtcacat catgttccag catatcttct gtcaacacgc gtttgatttg ttcgattggc
    28261 atgttgtatg tttctgataa ttcttggatt tctgcattga tttcttcttc tgttgcttga
    28321 agattttcag cagctgcaat tgcttcgatc acaagatttg ttcttgtgcg catttctgct
    28381 tcaccttcga attgtttatg caagtcttct tctgtagaac cagttaattg atagtacatt
    28441 tcaggagaga taccttgacg ttgcatgttg ttcaagaatt cgtccattga acgatgtact
    28501 tcatcatgaa ccatcacgtg tggcaattca acgatttctg cgttttcaac agccatacgg
    28561 atcgctgctt catcttttgc atcgtccgct gctttttctt ttgtttctac taattctttg
    28621 cgatattttt ctttcaattc gtctaatgtt gatacttcgt catcaacatc ttttgcgaat
    28681 tcgtcatcta attcaggcac ttcttttgct tttacttcat ggattgtcac tttgaagact
    28741 gcttcttttc cagcaagatc ttctgcttga taatcttctg ggaaagttac atttacatct
    28801 agactttcac ctgatttatg accaactaat tgatcttcaa aacctggaat gaatgagcct
    28861 gaacctaatt caagtgagta attttcgcct tttccgccat caaacgcttc gccatctaca
    28921 aagccttcga agtcgattac tactgtatcg ccattttcag ctgcagcatc ttctttgata
    28981 actaattcag cttgtgcttc ttgttcacgt ttgatacgtg catccacgtc agcatctgtt
    29041 acttcacggt cttgtttttc aacaactaga tctttatatt gtcctaattt tacttctggt
    29101 ttcactgtta cttcagcagt gattacccag tcttgacctt tttccatgct ttcaacatcg
    29161 atttttggtt gagaaacagg atcgatgcca gcttctttga cagcagcttc ataagcttct
    29221 ggtaatacag cattcaatgc atcttcatat aaagcttctt caccgtacat acggttgaat
    29281 acttgacgag aaactttccc tttacggaat ccaggtacgt tcaagctttt tttcacttta
    29341 ttaaacgcag ttgttaatcc tttttggatt tcgtcttgcg caatcgtaaa ttttaaaaca
    29401 ccatcattcg tgcctttttt ttcaaatgtt gcagtcattt aattccctcc gagtttagaa
    29461 attcatttta tacatcaaaa acgtttcatg cataccttta aatagtacac taaccctttc
    29521 aaattgtaaa catattgaat ggatagaatc cttgtttttc ataacatttt tatctttttc
    29581 actgataaaa tctaatggaa gaagatcatt tgctgcaaaa ttttttctct tttttttaaa
    29641 aaatcttgtt tttcatcttc tgtaccttca tacatcgttt ttcctgtaca cgcctctaca
    29701 gtcagttgaa accagcgctt cgggtcttcg atgtatgtat catagacagg ataaacaatt
    29761 gcagattgca acgtgaattc ttcttttaac acggaactaa gcagcgcatc ttcattcccg
    29821 taacgatctg aaaatacaca taattgctgg taacttgcct gctgctgcgg ctttggcaga
    29881 tcttttggta tgactttgac taacttttct tcaatcgtca aatagaaaac ttgatggcat
    29941 tcgttcaatt gcctaagtgt ctccagtaat tttgcccttg ctaaaatatg gacctctggc
    30001 gatacaataa gtttactcgc tgtttggata aactcatgat aaggaagctg ttcgatctca
    30061 tcaatagacg caagctgacg tgcaggttcc aacgaaggaa gttcctccga tttattgtgg
    30121 agtgccttga tcttctgcct ctccatttgt ccggataatt cttctgcaaa ttggattttt
    30181 tcaatgaccc gctgctcgaa agcttcataa aaatctgtac ttgctatcaa ttttctagca
    30241 tataaaaaat cttgtgtact gattaacaag tcaaaataaa attcagcaaa ctcctcattt
    30301 tgaagatact gtttttcatg caagagtgct tgcttcaatg cttctttttt ctcgtccaac
    30361 tcgtaaagac aaaaaacaag caggctatta gcctcaaagc tttctttcaa ctcgtaagct
    30421 cgttgaaata attcttttgc ctgctgatat tgctggtttg ctagtgcttg ttgtgccact
    30481 cgcattaaat tctcatattt ttctggaaaa tcaatcggct tcacgcagtg tcctcctctt
    30541 gctcatattc agctcatgtt caatagtagc aaaacaaaaa aagcatgaca attaaaaata
    30601 actgaagcac cctctaaaag ctagatttta gatctgactt ttagaaggtg ctttcaactg
    30661 ttactgcttt ttattgctca tcagctgttg acttcctggt tgaacgcttt gatcagattg
    30721 atcgtaacaa cacttgtatt agtgaaaaaa tcgcccagat gttgcttttt ctgattaaag
    30781 actgctggaa gataccctaa catcagcaaa ggaaatttta gaatgaaccg gcaagcacct
    30841 tctctaacta aaacggttga ccagctaagt tcttcttcat caaagctgat gactcgaata
    30901 ccaaagatca ttttcccaat cgtttggcca tgattgaatt tcgtcaataa aataaaatac
    30961 gctagataaa taagtaaggc tgtgaatccg tagagactca gataagaatc tgaaaaatta
    31021 agccaaccta gatttttggc aataccaagt gttaaacgcg taatagctcc gatacacaaa
    31081 agatcgacca agaaggcaaa cattcggata aaaaaacctg catagaaata cttaggaaaa
    31141 tcatttgctt ttggcggttc atttcttgtg tccgtatact ttttccattt acgttgtttc
    31201 tttttgattt cttcgggcgt caaaggctct ctttcaaaag agctcagtac cttttgggaa
    31261 aagttggagt caggtcctac agcaggctgc tgttgcttat ctttttcttg ttccttattt
    31321 tcatttgaaa gctctgacat gcttactcac ctccgtaata atacattgct tttggtgatt
    31381 ccgctgtccc tagattctca agaacggata agatctgact agtctcagaa ggcttcaatc
    31441 cctggaattc tgctagttta cttcctaacc atgtactagc aaacccagat ggcgtggaag
    31501 aatattctac tagttccgca tcttcaagct gttgttcctt tcgtatagct tttaaagcat
    31561 cttcaggaaa tcccaattca tcgactaaac ctacttcttt tgcttggaca ccatcataaa
    31621 tccttccatc agcaattttt ttgacttctt cttcagattt attccgtccc tcgctgacga
    31681 ttgctacaaa acgactatat gcgctgtcga tatatgcttg aagcaccgct tcttcttctt
    31741 ttgtctctgg tcgcgttcct gaacccatat ctttcaacgc tccgctttta actgttgtat
    31801 cttccacacc cagtttttct agaagtcctg aataattcaa tcctgacatg atcacaccaa
    31861 ttgagccggt cactgtttct tctgttgcaa agattttatc tgcttgagca gaaatatagt
    31921 agccaccgct agctgccatg tttttcatgc tgacatacat tgggatatca tgttctttac
    31981 gaatcgtatc caatttctta gcgatttccg cactttcgta gacaccgcca ccaggagaat
    32041 ttacttctaa aaagatacct ttgactgttg gatcttcttc aatcgccttc agttgttcca
    32101 taaaattttt atgattgtaa ccttcagaag caaataaacc tgaagaccct cccgcagcaa
    32161 tcgttccatc tactgataat tttacgattt tttgcgtact gtctccctct tccaacacat
    32221 ttggagctag ttcttcatcc ccatataacc aactgttcaa tccctcaatt tgatcgtttt
    32281 cctgaggctt agtcaaacta gctgaaacta acgaaaccac aaataatcct gcagcaatca
    32341 gaactgctgc ccaacgtctt ttattcattc tttctccttt ctaatacaag ttttttatga
    32401 cgatcacagt ttctgactgc tcagaagtgt cgaaccgctc tgctttttct tcccgcgcat
    32461 acccggaaaa cgactctttt gttaattcat cttctaccgt aaactcacga gcttgagcaa
    32521 cttccaaggc atcaggtgcg atttcttgga tgctatccca gccagtatct aaaaatattt
    32581 tcgtatcatc tgatacacca gctgatgctt cgaatttgga caattgtttt tttaacattc
    32641 ccagtgtcat gatctcaatt tttgaatcca tttttactcc tcctctatcc gattactttc
    32701 tattgtaccg aactcctgaa aaagatgata gataaaaacc cattattttt tgacataaaa
    32761 aggacatttg acaaagaatt tgatatatac caatttattt tttattcttt ttttgttgac
    32821 aagaacattc gcaaaaggtt atgattttaa atgtttccag ataaaaaaca aaaaggagga
    32881 tagttatacc ttttaggata gcgccaggtc tgtgtaaaca gatgacgagg agagggttta
    32941 tcgaaagatt cggcggatgg ccctagggac tgcactctac agaaaatcca caaaaaatgt
    33001 ctgcaaagat aaaacaaaag gattttcctg cagctggaag cattcatacg aattcattat
    33061 agaaagtagg atgtttttta tgtgcggaat tgttggaatg attggattaa aaaatgtaac
    33121 accaggattg attgacggtc tagaaaaatt agaataccgt ggttatgatt cagctggtat
    33181 ctttgtctca gatggcacaa ctgattatct ggtcaaagca caaggcagaa ttcaaaattt
    33241 gaaaaacaaa ataaatattg atactacagg aaatataggt attggtcata cacgatgggc
    33301 aactcacggg caaccttctg aagaaaacgc ccatccacat acttcccaaa gtggacgctt
    33361 cgtgctcgtt cacaacggcg taatcgaaaa ctttgaagaa cttaaaattg cttatttagc
    33421 aaatgaccac tttattggtg agaccgatac tgagattatc gctcatctaa ttgaaacttt
    33481 cgcgaaggat actacaacgc aagaggcgtt tttaaaagct ttgcgggtca tcaaaggttc
    33541 ttacgctttc gctttgattg atcggacagc acctgatgtt atttacgtcg ctaaaaataa
    33601 aagtccacta ttaatcggac tcggcgatgg attcaatgtg attgccagcg atgcaatggc
    33661 tatgcttgct catacgaagg aattcgttga gattgaagat gaagagatgg ttaccgtcac
    33721 atcagaaaaa gttattattc aaaattttgc aggtgacatc gtagaacgtt cttcattcga
    33781 agcacaagta gatgcttcag atatcgagaa aggtacctat cctttttata tgctgaaaga
    33841 aatcgacgaa cagcctatta tcatgcgtcg tatcgctcaa aaatacgtaa cagaagacaa
    33901 tcgtgtctct cttgatcaaa agctggtcga tacattgtct gatagcgatc gaatctatat
    33961 cgttgcttgc ggaactagtt accatgcggg gttagcaggt aaacagactt tggaaaaatt
    34021 gacacagatt cctgttgagg ttcatttagc cagtgaattt ggctataaca cacctcttct
    34081 ttcaaaaaaa ccattcttta ttttcttgag tcaaagtggc gaaacagcag acagccgcca
    34141 agtgctggta aaaatcaacc gtctcggcta cccttcactt acgattacaa atgttgctgg
    34201 ttctactctt tcgcgggaag catcctttac actgctattg catgctggac ccgaaatcgc
    34261 agtagcatca acgaaagctt atactgcaca aatagctgta ctaactttat tagccaaagc
    34321 tatcggtgat aaaaaagaat ttgaaacttc tcttgtattc gatatcactc atgaattgag
    34381 tttagttgct aatgcgatgg aatcggtcat cgcccaaaaa gattatttag aagagttggc
    34441 agaagtatat ctcagcgata caagaaatgc cttttacatt ggacgtggtg ctgactacaa
    34501 cgtttctctt gaagctgctt tgaaattgaa agaaatcagt tatatccagg cagaaggttt
    34561 tgccgctggc gaattgaaac atggaacgat tgctttgatc gaagaaggaa caccagtgat
    34621 cggaatcatc agtgaagaag tgactggtgc gcatactcga ggaaacctga aagaagtcga
    34681 aagccgtggc gctaaaacac tagttattgt ctcagaggga ttagaaaagg aaagtgacca
    34741 gctggtgcta ccaactgtcc acccttatct aactactcta accacagtcg taccgactca
    34801 attacttgca tattacgcaa cgctattacg gggattcgac gttgacaaac cacgtaattt
    34861 agcgaaatca gtgacagtag aataataaaa gaacaaggac cagtgtcaga catctgacac
    34921 tggtccttgt tcttttattt tttatgaaaa tatctcttaa tccagtccga tttcttcttt
    34981 gactactgcc gcgattttat cgacatagta attaactttc tcatcagaag gcgcttctgc
    35041 catcactcga agtaatggtt ctgtaccgga aggacgaact aggattcttc cttctgatcc
    35101 catttcagct tctgcctctt caatcacttt tttgattgcc ggaacatcca tcgcaccgtt
    35161 tttatcactt acgcggatat tcactaattt ttgcggatag attgttacct ctgctgcaag
    35221 ctctgataat ttcttacctg tctgtttcat gatattcagt aactgaattc ctgaaagcat
    35281 cccatctcca gttgtgttaa aatctaagaa aatcatgtgc ccagactgct cgccgccaaa
    35341 gttataatcg tttttgcgca tttcttctac aacgtaacga tcaccaactt gtgtaacgac
    35401 atctttcaaa ccgattcctt ccaccgcttt gtggaatcct aagttactca tgacagtcgt
    35461 aacgatcgta tcttttttca atcgattttt ttctgccaag tatttagcac agataaacat
    35521 gattttatca ccgtcgacaa tgtttccaag ttcatccacc gcaataatac gatcgccatc
    35581 gccatcgaaa gctagtccag catctgctcc tttttcaacg accatctcag ctaaacgttc
    35641 tggatgggta gagcctacgc cgtcattgat atttaacccg tctggatttg tgcccattgt
    35701 ataaaaatct gtttcaagat cggcaaacaa acgattaaca cttgttgaag ttgctccgtt
    35761 tgctgcatcg acacaaatgg tcaaaccgga aaggtcaccg ttgatcgttt gtacaaggaa
    35821 ttgagaatat ttcaataagc cttctggaaa ttcttctaca gttcccaatc cttcagctga
    35881 tggacgaggc aattcatcca cttcagcatc taataatgct tcaatttcta gctcttggtc
    35941 atctactagt ttaaaaccat ctgcaccgaa aaatttgatt ccattatctt gtgctggatt
    36001 atgagaagca gaaatcatga cccctgcgct tgctttttgc aaacgtgtca gataagcaac
    36061 acctggtgta gagatcacac ctagctggaa cacttcgata cccaccgata aaagtcctga
    36121 aatcagcgct tcttctaaca tttgacctga gattcttgta tcccgcccta ctaacacgcg
    36181 tgggcgtttt ccttcttctt catgctggct taggacataa cctccgcacc gtcccagctt
    36241 aaatgcaagt tctggcgtaa gttccttgtt ggcttcaccg cgtacgccgt ctgttccaaa
    36301 atatttaccc atagttgtaa aattactcct ttaacttaat tttctgagga atttgttctg
    36361 ctcatttgtg ttgattcgct tgtttctgat gtttcttctg tacttgtggt cgagctgctt
    36421 gtcccagatt ccgaactgga agaaatggtt gttgtcgatg gttccgttcc tgaatcagca
    36481 gatgaaatac ttctactcgt acttgtattt tcttgtgtcc gactgctcgt cgaagcagag
    36541 ctactgcttg acgtttgcaa cacgggtgta atctccacac taactgattc gggcttgata
    36601 cttaacccct cttctactgg tatttcaatt ttacgagttg ttcgatgatt gatacctgag
    36661 acattgatcg gtacagcaat actgtcaccg atcgatgcaa gcaaatcgct agagccgctt
    36721 acttctgctt ccagttttga aaaactgaaa tcatagcttt ctaccccggc agctggtgtt
    36781 ccttgctgta tcccataaag tcgtaccgac ttgcttggct tgatcgaatc agctctcact
    36841 gttacttgca ctggatcaga gacgatactt aatacttccc ctgctgcatt cagcgcctgt
    36901 attgggacct cactattaat cccatcatct gtgaccatcg aagcgtccac atcagctact
    36961 actcgtttga tttctgccaa tgttttgtca cctgtagtga tagagacttt tttaggacta
    37021 acggaagtat catcaagttc atatccagat ggtgtgaacg ttgaaggaat caacgtttcc
    37081 acatcgaaat cttttattac ctttttctca accgttactg taatagtcga tggttcgatc
    37141 tttgctgtga cagcactgct caaattttga atttttagcg gaacttcatg tgttccttcg
    37201 cccagcttag tgatatctgc tgtgacgcgg aacatccgtg tatcttcgtt tgcctctgca
    37261 ttcaactgaa ctcggtttgc actggaaagt ttgacagtca ctgcattttc atatccatgg
    37321 atatagtagc gctttgaatc ataagaaatc ttgatcggca cccccgtaac tgtttgatta
    37381 tacgcttcat ttccagaaag cgtatttgga acggactggc catttgcatt gaagtacagc
    37441 gcaatactga acagcaatgc aataaaactg taaagaatgc ttgtccgttt ctcttttgag
    37501 atcatttgcg atcccctccc ttagtcatac cgtctaggaa agattgaatc acgtttttct
    37561 tgttttttgt tttctcttca gcaattagtt ctctacgcaa gattgccaaa tattcttgct
    37621 gggataagcc agccatcagt tcattatcta acgtgatgct gacgtccccg gtttcttcag
    37681 agacaacgat ggtaatcgcg tcactgactt cgcttatacc gacagctgcc cggtgtcgag
    37741 tcccgaattc tttagggatc aacatacttt ctgatagagg aagataggcg cttgctactg
    37801 cgatctttcc ttcttttaca ataactgcac cgtcatgcag cggtgtatta ggaataaaga
    37861 tattgatcag cagctcccca gtgatatctg catctaaagc aatccctgtc tcaatatact
    37921 catctaatcc agtatgacgt tcgatagtga tcaacgcacc gatttttcgc ttggacatat
    37981 attggatggc tttgtcaaaa gacaagatca tcttctcatc ttcctgctgt tcgctttttg
    38041 attgtttgaa aagcgaactg cgtccaagat gttccagtcc tctccggatt tccggctgaa
    38101 aaatcacgat agcagctatc accccgtacg taatgacttg gttcatcagc caagacaagg
    38161 tgtgcagacc aatgatttcc gctacgattc ggataacaat aaaaatagct acacctttca
    38221 gaagctgaat cgctcttgtg cctcgcagca gttggatcag tttgtataca agataccaga
    38281 caaccagaat atcaagtatg ttcaccaaaa aattcagaga gaacaagtca ccggaaaaca
    38341 tttgctgcca atagtccaaa ttaaaaagtt ccccgatttg aaatgacata aaaagcctct
    38401 cttctctttt gagatttcct ctctagtata tcatatgttt taattttaga ggctgatatg
    38461 ctcgtatttt tcttgagaaa aacgcataat tttcaagaaa attgcaagta tgcgttttat
    38521 ttttctctgc a
//
LOCUS       DACDIG010000023        38219 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.023, whole genome shotgun sequence.
ACCESSION   DACDIG010000023 DACDIG010000000
VERSION     DACDIG010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.023"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            161..391
                     /locus_tag="IV247_001204"
     CDS             161..391
                     /locus_tag="IV247_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAP8777285.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            488..1789
                     /gene="murA"
                     /locus_tag="IV247_001205"
     CDS             488..1789
                     /gene="murA"
                     /locus_tag="IV247_001205"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP8777286.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1802..1978
                     /locus_tag="IV247_001206"
     CDS             1802..1978
                     /locus_tag="IV247_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP8777287.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2113..2409
                     /gene="yidD"
                     /locus_tag="IV247_001207"
     CDS             2113..2409
                     /gene="yidD"
                     /locus_tag="IV247_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAP8777288.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2473..2940
                     /locus_tag="IV247_001208"
     CDS             2473..2940
                     /locus_tag="IV247_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8777289.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3025..3909
                     /locus_tag="IV247_001209"
     CDS             3025..3909
                     /locus_tag="IV247_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8777290.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4026..4259
                     /locus_tag="IV247_001210"
     CDS             4026..4259
                     /locus_tag="IV247_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAP8777291.1"
                     /translation="MSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4347..4427
                     /locus_tag="IV247_001211"
     tRNA            4347..4427
                     /locus_tag="IV247_001211"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4381..4383,aa:Tyr,seq:gta)
     gene            4434..4505
                     /locus_tag="IV247_001212"
     tRNA            4434..4505
                     /locus_tag="IV247_001212"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4466..4468,aa:Gln,seq:ttg)
     gene            5020..5727
                     /locus_tag="IV247_001213"
     CDS             5020..5727
                     /locus_tag="IV247_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8777292.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5787..6431)
                     /locus_tag="IV247_001214"
     CDS             complement(5787..6431)
                     /locus_tag="IV247_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAP8777293.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6558..6845
                     /locus_tag="IV247_001215"
     CDS             6558..6845
                     /locus_tag="IV247_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777294.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            6953..7273
                     /locus_tag="IV247_001216"
     CDS             6953..7273
                     /locus_tag="IV247_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8777295.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7354..8667
                     /locus_tag="IV247_001217"
     CDS             7354..8667
                     /locus_tag="IV247_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP8777296.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATIQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8664..9383
                     /locus_tag="IV247_001218"
     CDS             8664..9383
                     /locus_tag="IV247_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAP8777297.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9511..10062
                     /gene="raiA"
                     /locus_tag="IV247_001219"
     CDS             9511..10062
                     /gene="raiA"
                     /locus_tag="IV247_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAP8777298.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10284..12818
                     /gene="secA"
                     /locus_tag="IV247_001220"
     CDS             10284..12818
                     /gene="secA"
                     /locus_tag="IV247_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAP8777299.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            12944..14045
                     /gene="prfB"
                     /locus_tag="IV247_001221"
     CDS             join(12944..13006,13008..14045)
                     /gene="prfB"
                     /locus_tag="IV247_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAP8777300.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14150..14836
                     /gene="ftsE"
                     /locus_tag="IV247_001222"
     CDS             14150..14836
                     /gene="ftsE"
                     /locus_tag="IV247_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAP8777301.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14820..15713
                     /locus_tag="IV247_001223"
     CDS             14820..15713
                     /locus_tag="IV247_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8777302.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            15865..17313
                     /gene="cls"
                     /locus_tag="IV247_001224"
     CDS             15865..17313
                     /gene="cls"
                     /locus_tag="IV247_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP8777303.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            17650..18507
                     /locus_tag="IV247_001225"
     CDS             17650..18507
                     /locus_tag="IV247_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAP8777304.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18622..19542
                     /gene="pstC"
                     /locus_tag="IV247_001226"
     CDS             18622..19542
                     /gene="pstC"
                     /locus_tag="IV247_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAP8777305.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19542..20426
                     /gene="pstA"
                     /locus_tag="IV247_001227"
     CDS             19542..20426
                     /gene="pstA"
                     /locus_tag="IV247_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAP8777306.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20441..21247
                     /gene="pstB"
                     /locus_tag="IV247_001228"
     CDS             20441..21247
                     /gene="pstB"
                     /locus_tag="IV247_001228"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP8777307.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21263..22021
                     /locus_tag="IV247_001229"
     CDS             21263..22021
                     /locus_tag="IV247_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP8777308.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22034..22711
                     /gene="phoU"
                     /locus_tag="IV247_001230"
     CDS             22034..22711
                     /gene="phoU"
                     /locus_tag="IV247_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAP8777309.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            22949..23980
                     /locus_tag="IV247_001231"
     CDS             22949..23980
                     /locus_tag="IV247_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP8777310.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            23977..24744
                     /locus_tag="IV247_001232"
     CDS             23977..24744
                     /locus_tag="IV247_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP8777311.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            24844..26412
                     /gene="liaX"
                     /locus_tag="IV247_001233"
     CDS             24844..26412
                     /gene="liaX"
                     /locus_tag="IV247_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAP8777312.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            26712..27029
                     /locus_tag="IV247_001234"
     CDS             26712..27029
                     /locus_tag="IV247_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAP8777313.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            27029..27379
                     /locus_tag="IV247_001235"
     CDS             27029..27379
                     /locus_tag="IV247_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAP8777314.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            27546..28484
                     /locus_tag="IV247_001236"
     CDS             27546..28484
                     /locus_tag="IV247_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAP8777315.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            28499..29329
                     /locus_tag="IV247_001237"
     CDS             28499..29329
                     /locus_tag="IV247_001237"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAP8777316.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            29373..30398
                     /locus_tag="IV247_001238"
     CDS             29373..30398
                     /locus_tag="IV247_001238"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP8777317.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHHASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            30413..31351
                     /gene="galU"
                     /locus_tag="IV247_001239"
     CDS             30413..31351
                     /gene="galU"
                     /locus_tag="IV247_001239"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP8777318.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            31476..31916
                     /locus_tag="IV247_001240"
     CDS             31476..31916
                     /locus_tag="IV247_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAP8777319.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            31919..32521
                     /locus_tag="IV247_001241"
     CDS             31919..32521
                     /locus_tag="IV247_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP8777320.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQGLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(32584..33687)
                     /locus_tag="IV247_001242"
     CDS             complement(32584..33687)
                     /locus_tag="IV247_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP8777321.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            33897..34898
                     /gene="ccpA"
                     /locus_tag="IV247_001243"
     CDS             33897..34898
                     /gene="ccpA"
                     /locus_tag="IV247_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAP8777322.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            35253..35648
                     /locus_tag="IV247_001244"
     CDS             35253..35648
                     /locus_tag="IV247_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777323.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREMQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGSYRIVILMTNKQDQSIYFSYSFWKERNEIGSY
                     GIVDEKKQKEGNTLNKVKVTYSNGNEESI"
     gene            35648..35899
                     /locus_tag="IV247_001245"
     CDS             35648..35899
                     /locus_tag="IV247_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777324.1"
                     /translation="MITDKQYSELSDAVYWLDPKHRDYVPEMQENLSFKINGTMYKIL
                     KIKNSFDGMQAMAVAPIVHSKLEKNFKNKKIPANFRVLK"
     gene            35942..36337
                     /locus_tag="IV247_001246"
     CDS             35942..36337
                     /locus_tag="IV247_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777325.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGSYGALVTMTNNEGKSVYFDYSFWKERNELGGF
                     GIENRAVQKTGVTIKRVKVIFSNGQEELV"
     gene            36334..37590
                     /locus_tag="IV247_001247"
     CDS             36334..37590
                     /locus_tag="IV247_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HAP8777326.1"
                     /translation="MTINDKHYNDISEKVYWLDPKYPRYNEGYKKNSVKEFAGMEFQI
                     LQIKDSLDGMQAMSVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDSYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGQLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            37602..37925
                     /locus_tag="IV247_001248"
     CDS             37602..37925
                     /locus_tag="IV247_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777327.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            37918..38184
                     /locus_tag="IV247_001249"
     CDS             37918..38184
                     /locus_tag="IV247_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777328.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNYQ
                     MTAEEAAQEFSNRHVHLKGSVKGQFAEKLTELFQRQEKKLKQFR"
BASE COUNT        12816 a         6499 c         8234 g        10670 t
ORIGIN      
        1 tttgtctcag tccctttttt tgcatatttt tagcatttat ttcctaaaaa atgtaagggg
       61 attcctatat ttttaagtat acttgttttt ccttttatac attttatggt attatcattt
      121 tattagttat tataaaacaa tcgttagaaa ggacgtcatt atgcaagtat acggaattga
      181 tgcggtgctt cgaatcgtga gtcatttggc gtttatctac ttagccttct ggtctttacg
      241 ttcgttacgc attgaaactt ttttcagacc aatgcataat acgcaaatta gaatggcaat
      301 cgtacttttt tcaatttttt tagggtacac tgcaagtaat tttttcttag aagttatcgc
      361 tttatgccga aatcttttcg ttagcctgta aattttaaaa aaaggtacta ttttaaaagc
      421 aaggatgtta taatagaaaa gattttgagt aagtgtatgc tcattggaat tttttggagg
      481 gaacaacatg gaagagatca tcgtaagagg tggcaatcaa ttaaatggaa cagtaagaat
      541 tgaaggagct aaaaatgctg ttttgcctat tttagctgct agtttgttag cagaagaagg
      601 tattacgaca ttagacaacg ttccgattct ttctgatgtc ttcactatga atcaagtgat
      661 tcgtcactta aacgtagatg tagattttga tgaacaaaag aatcaagtaa caatcgatgc
      721 ttcaagacaa ttagaaatcg aagcgcctta tgagtatgtt agtcaaatgc gtgcttcaat
      781 cgttgtgatg ggtccgctac ttgccagaaa tggacatgca aaagtagcta tgcctggtgg
      841 atgtgcgatt ggtaaacgac caatcgattt gcatttaaaa ggatttcaag ctttaggagc
      901 aaaaattatt caaaaaaacg gctatatcga agcaattgcg gatgaattga tcggtaacac
      961 gatttattta gatttcccta gtgtaggtgc tactcaaaat atcatgatgg ctgccgtaaa
     1021 agcaaaagga acaaccatca ttgaaaacgt agctcgcgaa ccagaaatcg ttgatttagc
     1081 aaacattttg aataaaatgg gcgctcaagt ctatggagct ggtactgaaa caatgcgtat
     1141 tgaaggcgtg gatcatttac atgcagtcaa ccactcaatc gtacaagatc gtatcgaagc
     1201 aggtaccttt atggttgctg ctgcaatgac acaaggcaat gtgctgattg cagatgctat
     1261 ctctgaacat aaccgcccat tgatttctaa attgatcgaa atgggtgcag aaatcattga
     1321 agaagaaggc ggagtccgtg tcatcggacc aaaacatatc ttacctacag atgtaaaaac
     1381 gatgcctcat cctggtttcc ctacagatat gcaagctcaa atgacagcta tccaattagt
     1441 tgcagaaggt acaagtgtag tgactgaaac agtgtttgaa aatcgtttcc aacatttaga
     1501 agagatgcgc cgtatgaacg ctcatgtcaa aatcgacgga aatgtagcaa tcatggatgg
     1561 aaaccatgaa ttgcaaggtg cagaagtata tgcaacagac ttgcgggcag cagcagcatt
     1621 agttttagct ggcttgaaag caaatggaat cacacgtgtg cgtaacttga attatctaga
     1681 ccgtggatat tataatttcc atatcaaact acagcaatta ggtgcagacg ttgaacgtgt
     1741 agacatggat caaacttctg ctgaaaaaac agcacaaacg attgcttaag gaagtggctg
     1801 aatgagtaac caacgatata tcttattaac tttgataaag attttagtgg tgatcttatt
     1861 gcttatcctt ctttttgtgg ccggaacgat gatcggatat ggtgtcatcg gtggcggcaa
     1921 tccatttaaa gtattccagc caagtttgtg gattcatatt cgagactttt ttcattgata
     1981 aataagaatc acagtggttc tagtcgatat gaagtgagcc atgaagcaga acatgacaaa
     2041 agaaaacaga agggaacgtt gacgttagtc gcgatccctt tttttagtaa aagaaaagaa
     2101 ggaatctaat tgatgaagaa gatattgatt tatttagtta gaggatatca gcgctttatt
     2161 tcgcctgcat ttccacctag ctgccgttat tacccaacct gttccaatta tatgataaaa
     2221 gcaattcaaa cccatggtgc actaaaaggg acggtaatgg ggactgcacg gatcttacgc
     2281 tgtcatcctt ttgtcaaagg cggtatcgat tatgtaccat tgacattcca attaacgcga
     2341 aataagaaag ataaaaaaga accagattat aaaaaggttg tgaaaaatac gagtagcact
     2401 gagcgataaa gaaaaagagg aaaattattg tcaaagtaac aactccgcaa ctacttatat
     2461 gggggacaaa aaatgatcga agaactaaac aagaaagatt taccatggtc cttgttatta
     2521 gatgcagatc ctgataaaga aaaagttcaa gtatatgttt ccagagggag tggctggata
     2581 tggaaagaaa aagaaaaaac aataggtgta ctgatctatg tggcaaggga aaaagaatac
     2641 gaaatcgtca atgtagcagt agccccaacg tatcaaggaa aggggattgg cggaaaatta
     2701 cttgagacag cttttcagaa gctttcacaa ctcgcttcca gtcaaactca gatcatcata
     2761 cgaaccggca gtactagcag tgcggcgtta catctctatc aaaaaatggg ctatgtggag
     2821 attggtcgag tcaaagacta ttttatccat cattactctg agcccatttt tgaaaatgga
     2881 gatcaattaa gggatcaagt aattttagca attcacttac ctaaaaatag ctttaactaa
     2941 taaactttca attcgagaaa atgctttcaa atgctaaata gatacattac aatagcttta
     3001 gcaaacaaaa aggagaatat ttttatggga atattagttt ttgattttgg tggatcggct
     3061 gtcaaatatg gttgttggga tggcaaagag attaaaggta agggacaatt tgcaacgcct
     3121 gaatcttggg aagaaatgaa agcgcaatta tttcaagttt ataaaaaaat actcgtttct
     3181 tttgaagtcg aaggaattgg aatcagctct cctggagtag tgaataatga gaaacaggtg
     3241 attgagggaa tcagtgcgat tccttatatt catggattta atattttccg agatttggaa
     3301 actctgtttc aactcccagt aacgattgaa aatgacgcca actgtgccgg aatggctgaa
     3361 ttttatgaag gtgctgcccg tgattatcaa gatgttgctt ttgttgtggc aggaactggg
     3421 attggcgggg cgttgttcac gaaaggacaa atcaataaag gggcacatct ttatggtgga
     3481 gaatttgggt tgatgttctt aaatggcgat aagacattca gtaaacttgg tacagcggta
     3541 cagatggctt ggcgttattg tgaacgcaaa ggtctcgata aaaattctta taccggaaaa
     3601 gatgtctttg aattagcaga aactggagat gcaatagcaa aagaagaagt tgagagtttt
     3661 tatacgtatc ttacaaaggg attattcagt attcaatttt cgtttgatcc agaagtgatc
     3721 gtattaggcg gcggtgtttc tgcaaaagaa gggctcattg atgagatcaa aagccgaatg
     3781 aagaagctga cagaacaatt cgatctgcat gatttcgaac cgcagatttt attatgtgaa
     3841 tacagaaacg atgctaatct tgtaggtgca gcagcaaact atctcgctgt acgtcaaggg
     3901 gagaattaag agctttagag aaggtgcagc ttgcataaaa gaagtgaatg aaatgaatag
     3961 gagattttta cctaaggaat gttttatttg ctgcagtatg ataaaatatc tgagaggtga
     4021 aaatgatgtc aaaaaaaaat aaagatgttg aagtgcgtat agaagaagtg aaaaaagata
     4081 tcaaaggtaa gaattatgaa gtcactcagt tatttatagg gaaaaaaatg attggtgaga
     4141 tcttagccta tgggccaaaa gaatatgaaa ttttctttgg tgaagaagat tttggaaaag
     4201 aaaaaagtct tgaaaatgcg atagaaacag tgattcgaca ctggaatctg cacgaataaa
     4261 aaaatatcaa aaaaactttc aataaagggt tgcaaaaatc tggaaaatta ggtattataa
     4321 ctaagtcagg ttgatacaaa cctttcggag gagtagcgaa gtggctaaac gcgacggact
     4381 gtaaatccgt tccttagggt tcagtggttc gaatccactc tcctccattt cattggggta
     4441 tagccaagcg gtaaggcaag ggactttgac tccctcatgc gttggttcga atccagctac
     4501 cccagtatac cacaatcgta agctctcgtt agttcgagag ctcttttttt tgctttgcta
     4561 ccttgttcct tgaagattct gctgatttaa gatagagtta agttaactac aagaattgga
     4621 gggatccaca tgaaatggaa agaaaaagta gacgaagcag ctgaaaagtt atatgatttg
     4681 gtcaaatcag aaaaatacaa tattgaagtg aatattccga aaaaaggcgg aaaagcggtc
     4741 caggtaaaat caaaacgccc aacgaatcat acaaaaaaat ggatggctaa aaaccgctaa
     4801 taataacaaa agatccataa gtcggcattt tcatcgattt tagggtcttt tgttattttc
     4861 taggttgaac atatctaaaa aattgtttct gacaatagga gtaggaagaa cgatgtgtgt
     4921 ctaaatcctc tgttgatgga tagaaacctt tatctttttc agttgtgaat caggtatata
     4981 ccagttataa tagaagaaac gaaaggacgg gtgtacgtta tgtcgaatca attacctgta
     5041 tatatacaga ttcatgacca attaaaaaat gaaatcgaac aaggtatatg gaaagtcgga
     5101 gaccgtcttc cttcagaaag agaattagca gtgaaattca atgtcagccg tatgacgttg
     5161 agacaagcaa ttcaaaactt ggcagatgaa ggaatcttag aaagaaagat cggttcgggt
     5221 acatatgttg cgcgagaaaa agtacaagaa aaaatgtctg gtgcaacaag ttttacagaa
     5281 attatggaat cgcaaaatcg agtaccgtct agtcggacaa tctcttattt cttgacttcg
     5341 ccaagctcaa gtgaaatgga aaaactaaac ttgagtaaag atgacacgat cttgcgaatg
     5401 gaacgtattc gtttcgcaga tgatgtccct atttgttttg aagttgccag tgtgccacaa
     5461 aagctgatcg atggctacag caaagcagag attactgaat cgttgtatcg cacgctagaa
     5521 gaaaaaggcg ggaaaaagat cggtccagcg aatcaaacga ttactgctgt gttggcttct
     5581 gagaaaattg ctgactattt agaggtaaaa cgaggagatg cagttcttcg tttgcgccag
     5641 atttcttatt ttgaagacgg tacgccgttt gagtatgtgc gtacgcaata cgtaggaaat
     5701 cgttttgagt tttatttgga aaaataaaaa acaggtgatc cctgattcct tacttagaaa
     5761 taaaagagaa tcagaatcac ctgtttttat atatgatcag ttaccagttg ctcgtgataa
     5821 gaaacaggac cctcttcaaa ttggacttgt ccgttcagca gctcaatgat ggattcttta
     5881 aagtggtcag cacttttttc atctacagca cagatgacgc taacttgatc agtatattgg
     5941 atttctttta taaaaaaatg ttggagttca agataattct gtaattttcc tagttgcgga
     6001 tacgtgatca tcacagagat ctcctgttgc aaggtacctt ctacgattcc aatgtcagac
     6061 aatgcttgag agacagcatg agtgtatgct cgtattaatc cgcctgtgcc gagtttcgtt
     6121 cctccaaaat atcgagtgac tactgcagct acattgatca gttgattttt tttgaggact
     6181 tccaacatcg gtacgccagc cgttccactt ggttctccat catcgctgct tcgctggatt
     6241 tcattttttt ctccaatgat gaaggcgctg cagttatggt tcgctttcca atgttctttt
     6301 tttatttctt gaatgaaact ttttgcttgg tcctctgatt ctattcgtct aagagaacag
     6361 ataaagcgtg attttttgat ctcaatctca ctttgtccat tttcttttat tgtacgatag
     6421 cgctcgatca tcttgtcacc tctttttttc attttcttta ttcagtataa ataaagttct
     6481 aggttgtttc aacactgtaa cggttgcgca atcgctgtag tatgcgataa taaagacaaa
     6541 ggtaggtgga tctggggatg caggaacgaa tgaaaaagta cgatgcaatt acgcattatt
     6601 tgaagaacaa tggcggctct caagtaacgt tgacctttac ccaatttgac gaactgctgt
     6661 ttcccagtaa cggacttccc aaaacagcaa gggaaagtac agattggtgg gcaaatgatt
     6721 ataaacaccc tgaaaaaggt gcctatggct ggataaatgc aggctatgaa gtagttgtca
     6781 tcaatttgga caaagaatat gtcgtgttca ataaattagt gaaatctagt tggctatttg
     6841 attaaataaa ccgcgtttag aaaacgttga caaatttctt tctttttata taatagagta
     6901 acagacgcga agaaaacgtt tgcgtagttt gttttaaaaa ggagagaaaa gtatggctaa
     6961 aaaaacaatc atgcttgtat gttcggctgg tatgagcaca agtttactag taacaaaaat
     7021 gcaaaaagct gctgaagcaa aaggaatcga ctcggatatt ttcgctgtgt ccgcttctga
     7081 cgcagataac aatcttgcaa ataaagatgt agatgtactg ttattaggac ctcaagttcg
     7141 ctttatgaaa gctgattttg aaaaacgtct ggaaccaaaa ggcattcctt tagacgtcat
     7201 caatatggct gattatggta tgatgaatgg cgaaaaagtg ttagatcaag caatcacgct
     7261 cataggtgaa taaatagaac agtataacta tcgaacaagc gatccaaaaa atagatcgct
     7321 tgttttttcg tatatttttt tagggcgaat aatatgaatc atttttgggg tagaaggctt
     7381 ctgacgagag atattaaagc aatagactgt gaggaggtaa atttaccaaa ttatgagaag
     7441 atcgcagcag tggaacgttt gggcaactgg attctttgtc atcgctgtgg aatgaggacg
     7501 tccgtcattg atggacagct tgcagattac ggttattttt gtacacattg tctatattta
     7561 gggcgatgcg acactcaaca agatttatat ttgtttgatc agcctgaagc aaaacctcga
     7621 gaagtcgtct ttttttggac aggacagctt actgaaaaac aaacggaaat cgcaaagaga
     7681 atactttgcc attcagaaaa aagacatcat cttatttggg ctgtaacagg tgcagggaaa
     7741 acagagatgc tttatcctat tttagtggaa actttaaaag ctggaggaag agtagcgatt
     7801 tgtacaccgc gagtcgatgt ttgtaatgaa ttgtttttac gctatcgtta tgtttttcca
     7861 gaagaaaaaa tcacgttgct ccatgggaat acagcaacgg cttatacctt tacttctttt
     7921 gttatctgta cgattcatca gctataccgt ttttatcggt cctttgattt aatagtgatc
     7981 gatgagatcg atgcgtttcc ttataacggg gatgcaggat tagctcatgc tgtgcaaaca
     8041 gcgattaaac cagacggacg attcatctac ttaagtgcca cacctgataa aaagttacta
     8101 aaggaaatca agcagacgtt tgacatccac aaacttgctt tgcgctttca tcggcgttta
     8161 cttcctgaac ccgtcttggt tttcatgaat aattggcgac aaaaatgcaa gaacaagaag
     8221 aagataaaat ctttggtgcg gatcattcat caattaatta aagaaaacca tatcctttta
     8281 ttttgtccaa gtatcacgat gatgcatcgt ttgaaagcca caattcaaga ggctcttccg
     8341 agatatgaga tcacagatgt ttcttcaaga gacaatcagc gggcggaaaa agtccagaaa
     8401 atgcgagaag caaaatatga catactgtta accacgatga tccttgaacg tggagtcaca
     8461 tttgaaagaa tatccgttat cgttttagga gcggatcatc cggtttttac taaatcttct
     8521 ttggtacaaa tcgcaggaag agccgatcgt aaagggcctt atacgaacag tcaagtatac
     8581 tttttctatg aagaaaaaac tgcagctatt cgaaaagctt gtcaggaaat caaagaaatg
     8641 aatgaagaag ggaagaagat gctgtgaggt gcagttgctg cagtaaagat atcgtccaaa
     8701 atttaacatt cagcgagatt attggtgttt cgaaatacag attacctcag acttgttcag
     8761 tttgtcgcgc acaatttcat ttattggaaa caagaggctg ttctggctgt caaaaaccaa
     8821 tccatttcaa tctagagagt acgaaaaaga aagtaaagtc tattcccgtt tactgcagtg
     8881 actgtcaaga atggaagaag aaatatccga cctatgattt tcagcaccgt gccttttttg
     8941 tgtacgatga aaatatgaag gcctggctca atcaatacaa gtttatggga gatatccgac
     9001 tagcaggcac ctttgcatat aaatggacgc agataaaaaa agaatacccg acatacatct
     9061 attgtccaat acctttatct gaaaaaaggc gaacagaaag aggattcaat caagtaagcg
     9121 agatgttgaa agcagcaaga attccttttc agatgctact caaaagagag aagcatctgc
     9181 tggctcaagc aaaaaacacg cgaaaggaaa gattggagat gcctcaacct tttgaacttg
     9241 cagtcacctt ttctaagata caaaataaaa aaatattgat tattgatgat gtctatacaa
     9301 caggacagac tcttttccac gcagcagatt gtttgcaggt cgcacagccg aaaatcatcc
     9361 gaaccatttc tttagcaaga tgaattagat gtggaaattt gatagcgatt taatttgcaa
     9421 ataacctaga gttcgatata atagaattaa gaagagggaa agagtggtcc ttctccttct
     9481 tgattgtggt agacgaaagg ggtaattttt atgtttagat ataatgtacg cggcgaaaat
     9541 atcgaagtaa ctgaagcgat tcgcgactac gttgagaaaa aagtaggcaa actagaacgc
     9601 tatttcagtg acgcacccga tgcaacggcg catgtgaact taaaagttta tacagaaaaa
     9661 acagcaaaag tggaagtaac gatcccatta ccttatcttg tattacgtgc cgaagaaact
     9721 tctccagatt tatacgcaag tatcgatttg gttgttgaca aattggaacg ccaaatccgc
     9781 aaattcaaaa caaaaattaa tcgtaaatcc cgtgaaacgg gcatgaatac tgcagatgct
     9841 actgctttat tcaatgaaga ggacgaaaac gaaaatgatt ctgaattaga tatcgtacgt
     9901 acgaaacgcc tttcattgaa accaatggat agtgaagaag cagtcttaca aatgaacatg
     9961 ctgggacata acttctttat ttttgaagat gcagaaacaa atggtacaag tatcgtttac
    10021 cgccgtaaag atggaaaata cggactgatt gaaacagact gatcagcctt acttaaataa
    10081 caacgaaaac ttgcagcagc tactgacagc tgccgcaagt ttttttgagt ttcctcctct
    10141 tttagatgaa atatgaataa attatgaagt tcttacttta atacgcttaa gatatttcaa
    10201 ttatctataa ctaatgataa aatgaaaagt aaagtctaca tagtctggat aatacacaga
    10261 aggaaaagga gattacacaa cgcatggcca atttgattcg ttcacttatc gaaaacgata
    10321 agaaagaact aaaacgttta gatagcattg caaaaaaagt tgaagcatac gctgaccaaa
    10381 tggcagcatt aactgatgaa caattacagg caaaaactgc tgaattcaag ggcagatacc
    10441 aaaaaggaga aacattagat caattacttc cggaagcttt tgcagtcgtc cgagaagcag
    10501 caaaacgtgt attggggtta tatccacacc acgtacaatt gatgggtgga atcgtgcttc
    10561 acgatggaaa tatccctgaa atgcgtactg gtgaaggtaa aaccttgacc gcaacgatgc
    10621 cagtatatct aaatgctctg actggcgaag gggtacacgt agttaccgtc aatgaatatt
    10681 tagcaacccg tgactcgacg gaaatgggcg aactttataa tttcttagga ttaacagtgg
    10741 ggttgaatat caactcaaaa tcttcggatg aaaaacgcga ggcttataat tgtgatatca
    10801 cttatagtac gaataatgaa ttaggatttg actacttgag agacaatatg gtcgtttacc
    10861 gccatcaaat ggtacaacgt ccgctgaatt atgccattgt cgatgaagtt gactcaatct
    10921 tgattgatga agcgagaacg ccgctgatca tttcagggca agcagaaaaa tccacagctt
    10981 tatatacaag agcagacaac ttcgtgaaac gtttgaaaga agaagaagat tataaaatcg
    11041 atgtacaatc aaaaacaatc agtttaaccg aagcagggat cgaaaaagca gaagaaacat
    11101 tcggtttgga taacttatac gacattgaaa atactgcatt gacccatcac ttggatcaag
    11161 cattgagagc caactatatc atgcttttag atatcgatta tgttgtccaa gacggaaaag
    11221 tactgattgt tgaccagttc actggtcgga tcatggatgg acgccgttat tcagatggac
    11281 ttcaccaagc aatcgaagct aaagaaggcg tagagatcga agacgaaacg aaaaccatgg
    11341 cgacgatcac tttccagaac tatttccgta tgtacaagaa acttgcggga atgactggta
    11401 cagctaaaac agaagaagaa gaattcagag aaatttataa catccaagta taccagattc
    11461 cgacaaatcg tccggttatc cgtgatgatc gtccagattt gctttaccca acattacaaa
    11521 gcaagttcca cgctgtcgtg caagatatca aagatcgcta ccgcaaaggt caacctgtgt
    11581 tagtcggaac tgttgcggtt gaaacatctg agttattatc agatatgctg aatcaagaac
    11641 gtattcctca tgaagtgctg aatgcgaaaa atcactttaa agaagcagag atcatcatga
    11701 atgctggtca aaaaggtgct gtgacgattg ctaccaacat ggctggtcgg ggaacagata
    11761 tcaagttagg acttggggta agagaacttg gtggtctagc agttatcggg acagaacgac
    11821 atgaatctcg tcgtatcgat aaccagcttc gaggacgtgc aggacgtcaa ggagaccctg
    11881 gtatgtcaca gttctacttg tcattggaag atgatttgat gaaacgattc ggttcagaac
    11941 gaatcaaatc tttcttagat cggatgaaga tcggtgagga agatgctgtt atccaaagca
    12001 agatgctgac gaaacaagta gaatctgcac aaaaacgtgt agaaggaaat aactatgaca
    12061 cacgtaaaaa cgtcttacaa tacgatgatg tgatgcgtga acaacgtgaa gtaatctata
    12121 aacagcgaca agaagtaatc atggaagaaa aaagcttatc caagccacta atgaacatgg
    12181 tcaaacggac aatcagtcgt gtagtagatg cgcatacaca attagagaaa gaaaattgga
    12241 atttggaaag tatcatcgat tttgccggca gtactcttgt ccatgaagat agtattactc
    12301 ttgcagatat cgaaggaaaa acagctcagg agatcaaaga gtatctaaac gaacgagcac
    12361 aagaagtatt taataccaaa gcggctcaac taaatggtcc agagcaactg ttggaatttg
    12421 aaaaagtcgt gatcttgcgt gttgtggact ctaaatggac agaccatatt gatgcaatgg
    12481 atcaattacg tcagtcaatc ggcttacgtg cttatggaca gaacaatcca ttagtcgaat
    12541 atcaaacaga aggctataag atgtttgaag atatggtagg cgccatcgag tatgaagtca
    12601 ctcgaatctt tatgaaagct gagatccgtc aaaacgtaca gcgtgaacag gtttctcaag
    12661 gaacagcttc tcactcagaa gacggagatg ccaaagaaaa tagtaatact agcgcgaaaa
    12721 aacagccaat ccatgtgaac aaggtcggaa gaaatgatcc ttgtccttgt ggaagcggga
    12781 aaaaatataa gaactgtcat ggaaaaaatg caaactaatt tctgacagca ggattggtga
    12841 gacaaattct ttttttaaga attcgtctca ccttctttct gttatttgct gattctgtta
    12901 gactagatat aatgaaaaaa gtatagtgaa agaaggaaaa aacatggaaa tatccgaaat
    12961 tcgaaatctc ctgacagaaa tgcaggaaaa ggtcacaagc ttcaggaggt ctctttgact
    13021 tagaccagct ggaagaagac attgcggaag cagaagcaaa aatggctgaa cctggttttt
    13081 gggatgatag cgaagctgct caaaaagtga tcagtgaaaa caatgcaaat aaagaaactt
    13141 atgatcaatt ccattctctt gctgaagaac tggaagaaat cgaattgatg gtggagatgt
    13201 tgcaggaaga acctgatgca gccatgcaag aagaagcaga aacacgtatc aatgcgctag
    13261 atgaaaaaat gaagacatat gaattatcta tgttgttaga tggtccttat gatcgaaaca
    13321 acgccatcgt tgaattgcat ccaggggctg gcggaacgga gtcccaagat tggggcagta
    13381 tgcttttacg tatgtataca cgctgggcag agcagcatgg gtatcaagta gagacactag
    13441 attatcaagc aggtgatgaa gcaggaatca agagtgtgac cctactgatc aaaggacaca
    13501 acgcatttgg ttatctgaaa tcagaaaaag gtgttcatcg ccttgtacgt atctcgcctt
    13561 ttgactcagc gaaacgacgt catacttctt tttgttcggt agatgtgatg ccagagcttg
    13621 atgaaacgat cgatatagaa atcaatccag atgatttgaa aatcgatacg taccgagcaa
    13681 gtggtgcagg tggtcagcac atcaataaaa cagaatctgc tgtacggatc actcacttgc
    13741 ctacaggaac agtagtagct agtcaggcac aacgttcaca attgaaaaac cgtgagcaag
    13801 cgatggggat gttaaaagct aagctttatc aattagaagt ggaaaagaaa gaacaagaag
    13861 cagctgcact tcgaggagaa caaaaagaaa tcggctgggg ttcacagatc cgttcttatg
    13921 ttttccatcc atattctatg gtaaaagacc atcggacaaa cgtagagaca ggaaatgtcc
    13981 aagcagttat ggacggtgac cttgatttat ttattgacgg ctatttaaaa atgcatctac
    14041 aataagaacc cattgttaaa ttgaaataaa attgtaagat tttgcaagca tgttgaaaca
    14101 ttgccatgct ataatagcta aagactgaaa aagatatgga gagaacgcca tgattgaaat
    14161 gaaggatgta atgaagaagt actcaaatgg tacgaccgct atccgcaatc tctcaattgt
    14221 catcgaacaa ggagagtttg tctatgtggt tggtccgtct ggagccggta aatctacatt
    14281 catcaaattg atgtatcgag aagaaaaagc ttcaaaagga actttgaacg tcgcaggtca
    14341 tgatctaata gcaatcaaaa accgcgaagt tccttatcta cgcagagaaa tcggcgtggt
    14401 gttccaagac tataaattat taccaagaaa aacagtatat gaaaatgttg cgtacgctat
    14461 gcaagttatc ggcagaaaac ctcgggatat caaaaaacga gtaatggaag tgctggacct
    14521 tgttggtttg aaacacaaag ttcgagtctt tcctagcgaa ttatccggtg gggaacagca
    14581 acgtgtctct attgctcggg caatagtgaa tacgcctaaa gtacttatcg cggatgaacc
    14641 gacaggaaat ctggatccag aaaactcatg ggaaatcatg aaattattag atcggatcaa
    14701 tgcccaagga actactgtcg tgatggctac acataacagt acgatcgtaa atacgattcg
    14761 gcaccgagta attgctatcg aaaacggccg cattattcga gatcaagcgg aaggggaata
    14821 tggatacgat gattagaacc tttttccgcc atttattaga aagtatcaag agtttgaaac
    14881 gtaacggctg gatgacagtt gcttcagtca gtgcggtaac gatcacattg actttagtcg
    14941 gtgtattcat ggcagttatc atgaacgcaa ctaaattagc tcaagatata gaaggaaacg
    15001 ttgatgtctc tgtattcgta gatatcggta caaaagacga agagatgaaa aagctagaaa
    15061 aagaattgaa tgacttagat catgttaaaa aagttgaatt ttcaagcaaa gaacaggaat
    15121 tgaaaaaaat ccaaaacgaa atgggtgatt cctggaaatt gtttgaaggt gacaataacc
    15181 cgttatatga tgtgtatgta gtaagtgcta ctgatccttc ttatacaaaa caagtagcag
    15241 aagaagcagc agatttgaag aatgtttctc gttctgatta cggtggagct tcctcagatc
    15301 ggattttcca aatcgctggt gcagttcgga catggggatt agttgcagcg gcattgctgc
    15361 tgttcgtagc gatgtttctc atttcgaaca cgattcggat cacgattttg tctagacagc
    15421 gtgaaattca aatcatgcgt ttagtcggtg caaaaaatgg atatattcgc tggccgtttt
    15481 tccttgaagg tggctggatc ggtctgatcg gtgcgattct tccgattttg atcatcacat
    15541 ttggttatgc atggtttttc caattgatca atccatcatt attacgttcc cattattcat
    15601 tgattcatcc acaaaatatc gtttggaaaa tcaatctttt aatggtcggc attggtgtga
    15661 tcattggttc acttggctcg atcatttcta tgagacgttt cttgaaattc taataccaca
    15721 atataaataa aatgagaaag ttggacagtt tcgttccagc tttctttttt tattggctag
    15781 gagaatagaa ggaagcggct tttttccctt tactttaaaa aacaacttgg gtattcttaa
    15841 gttggtagaa gaagaggtga agccgtgaaa attatcgttg acaatttttt tacgatcctt
    15901 ttgattatga atattttgct ttcctttatc atcgtttttc gagaaagaaa ggaaactgct
    15961 caaacatggg catgggtgct cgttttgatg ttcattccag ttgtaggatt tattttatat
    16021 atttttttag gaagagggat ttccaaagag aaaatctttg atttgaagat ccaagggaaa
    16081 atcggtaaga acctggaaat tgaagaagac aaacaggcat tgatgcgagg gctttatcct
    16141 catccgccta ctggacaagt agatgtgaaa caattgatct atatgctcac ggtatttgaa
    16201 agtacgctct atacgactaa aaatgaaatc actttattta cagatggtcg tgaaaaattc
    16261 gatgcgctga ttgaagatat caagcaggca gaagaccata tccacttcca gtattatatt
    16321 tatcgcagtg atgcattagg tgaagaagtt cgagatgcat tgacagaggc ggcaagacga
    16381 ggggtaaaag tcagagtcct tcttgatgca tggggttcca cacaagtttc ttccagcttt
    16441 tttcagaatt tgaaaaaagc aggcggagaa gtcgcatttt tctttcctct atttgttccg
    16501 tatattaatc ctcggatcaa ttatcgcaat catcgtaaaa tcgtagttat tgatgggaaa
    16561 atcggatata caggtgggtt caatgttgga aatgaatatc taggattggt aaagaaattt
    16621 ggttattggc gagacaatca tttgcgtatt tacggagaag cggtgtatac cttgcaaaac
    16681 cgttttttga tggactggaa ttctcagcat acaaaagaag taggctattc tcccaaattt
    16741 tttccgtcca ttcactctac tggtgatata gctgttcaaa tcgtgacaag cggaccagat
    16801 accgaacatg aacaaatcaa aatgacttat ttgaaaatga tttcgttagc caaacgggag
    16861 atattgatcc aaacgcctta ttacataccg gatggatcga ttcatgaagc actgaagttg
    16921 gctcttcttt caggcgtaaa agttcatatc caaataccta acaaaccaga tcatcttctc
    16981 gtttactggg caacgtattc tttcgctgca gagctaatcg agtatggagc aaggatcgaa
    17041 acatatgaga atggatttat tcatgcaaag acgatgatta tcgatggtgg cattgtttca
    17101 gttggctcag caaatatcga tgttcgttca tttcgcttgg actttgaggt caatacactg
    17161 gtttatgatg aacgaatggc ttcacgtgta agaaaagctt tttttgaaga ctcgaaaatt
    17221 tctacgcatc tcacaaaaga aatgtatgaa aaccgaggaa taatcatcaa gatgaaagaa
    17281 ggattagcaa gattgatttc gccgctcctt taaaacataa aaaggacatg agaaccatca
    17341 acagatggag atctcgtgtc cttttctaac ggggccattt ctgagagtac gtcagaaatg
    17401 gagaaagcgt ctcgcatgag cgaaattaac tttggggtat ctcttgtttt ttggggaaag
    17461 caagaaataa gctaatatat gctattaaaa gagacacatt tcaaagccag tatagcatag
    17521 aaacttttaa tgagatagtt ttttttacaa ttatgagaat ttattttgtt atttaatgat
    17581 tttgattcgt tttcatcaag taatgaaaat gataaaataa acatacttta ggattaaagg
    17641 agatttctaa tgaaaaaact taaattattc ttaccaataa taggattagc tattctttta
    17701 tcaggctgtt caaagtggat tgatcgagga gaatccatta cggctgttgg ctcttctgct
    17761 ttacagcctt tagtagaaac agttgctgaa acttaccaga cagaaaatcc tggaaaattc
    17821 atcaatgtac aagggggcgg aagcggaact ggtttgtctc aagtacagtc aggagcagtc
    17881 gacatcggaa actctgattt gtttgcggaa gaaaaatcgg ggatcgatgc ctcagcatta
    17941 gtcgatcatc gagtagcagt tgttgggatc actcctattg tgaacaagga tattggcgta
    18001 aaagacatca gtatggaaaa cctgaaaaaa ctatttacag gcgagatcaa aaactggagt
    18061 gaagtcggtg gaaaagatca gccagtagtt atattgaacc gcgcttcagg aagcggaacc
    18121 cgtagcacgt ttgaaaaatg ggtattggac ggagaaacag ctgttcaggc acaagaacaa
    18181 gattcaagcg gaatggtgcg ccagatcgtt gccgacacac ccggtgcaat cagctatgtt
    18241 gctttttctt atgtttccga tgatgtagat acgttgaatg ttaacggtgt agaaccgaca
    18301 gatgaaaatg tgacaacgaa caaatggacg atttgggcat atgagcatat gtacacaaga
    18361 gatcatccaa aaaaactgac aaaagaattt ttagactata tcttatctga tgaagtacaa
    18421 gataacatcg ttggagaact aggttatatc cctgtttctc atatgaaagt cgaacgtgat
    18481 tggcaaggaa atatcgttaa agagtaatct ttacacaaaa tttacaaact tagctaaact
    18541 tttacatgtt attaaaacca aattgatgtt caatttgtac actgagtgag tatgttaaac
    18601 ctgattaagg gggagtcatc tgtggaagat gtgaaaaaag tattattaac aaaatcaaaa
    18661 cgttcaaaaa tggaacagcg tggaaaattc atcagttttt tatgtatcgc gctgatcgtg
    18721 ttggttgtat tatctatttt ttattttgta gctagtaaag gattggccac cttttttact
    18781 gataagatca atgtatttga ttttctgttt ggtacagatt ggaatccaag ccaaatcgga
    18841 gcaaatggga aaccaatggt aggggcattg cctatgatca caggttcgtt tattgtgacc
    18901 tttttatctg ctatcgttgc cacgccattt gccattggtg cagcagtatt catgacggag
    18961 atctctccta aaaacggaac gaagatctta cagccggtca tcgagctgct tgtaggtatt
    19021 ccttctgtcg tttatggttt tatcggttta tcagttatcg tgccttttgt cagaagtatt
    19081 tttggcggga ctgggtttgg tatcttagct ggtacatttg tgttgttcgt tatgatcttg
    19141 ccgactgtca caacgatgac tgttgatgca ttaaaggcag tgccacggca ctatcgggaa
    19201 gcttcattag cgcttggtgc cacaagatgg caaacaatct acaaagtcgt gctacgagca
    19261 gccgtgcccg ggattttaac agcagtcgtt tttggtatgg ctcgtgcgtt tggtgaagcc
    19321 ttagctatcc aaatggtcat cggtaatgca gcattgatgc caacaggtct tactactcct
    19381 gcttctacat tgacgtccat cttaacaatg ggaatcggaa atacaatcat gggaacagtt
    19441 gaaaataatg tgttatggtc attagcgctg atcttgctat tgatgtcatt attctttaat
    19501 atcgtgatcc gtatgattgg gaagaaagga gagctgaaat aatgaacgca aaacgctcag
    19561 ataaaattgc tacaggtgtt ttatatatag tttcggggat catcgtattg attttagcag
    19621 ctttactact ttatatctta gttcggggaa taccgcacat ttcatggtcc ttcttgaccg
    19681 aaccttccaa agcctaccaa gaaggcggtg gaatcgggat ccagctattt aactcgattt
    19741 atttgctttt gatcacgatg atcatcagtt ttcctatttc tttaggtgcg ggtatctatc
    19801 tttcagaata tgctggaaaa aactggctaa cagatgtgat tcgcacgtcg attgaaatct
    19861 tgagctcgtt gccttctgtc gttgtcggtt tattcggttt cttggttttc gttattcaat
    19921 tccagtatgg tttctcgatt atttctggtg cattggcttt gactgtattc aacttgcctc
    19981 ttttgactag aacagttgag gagtctttac aagcggtcca ttacacacaa cgagaagcag
    20041 gattggcctt aggtttgtca cgctgggaaa cagtgataaa agtggtggtt ccagaagcga
    20101 caccagggat tttgactggt gtgatcctaa gttctggaag aatttttggt gaagcagctg
    20161 cattgatcta tacggctgga caaagtgcac ctgcgttaga ttttacgaac tggaatccat
    20221 taagtgtttc aagcccgatc agcattttcc gtcaagctga aacattagct gtccatattt
    20281 ggaagatcaa tacagaaggc acgatgcctg atggtgtttc tgtttcggct ggcgcttctg
    20341 ccgtgttgat tattgctgtt ttactgttca acttcggcgc tcgtgcgatt ggcaaacgat
    20401 tataccgaaa aatgacttca gcctaaggag aagaagcaaa atgaaaaaat ataactggaa
    20461 tgaaacaaat gtcatcacca tgccaaaaga gaaagaagta gcactatata cggatgatct
    20521 acatgtctgg tatggcgaca atgaagcaat caaaggagtg gatctggaat ttgaaaaaaa
    20581 taagatcact gcactgattg gtccttccgg ctgcggaaaa tctacgtatc ttcgttcgtt
    20641 gaatcgaatg aatgatggga tcgctaatac gaaaatcact gggaaaatca tgtatcaagg
    20701 cgtagatatt aatgcgcctc aagtggatgt ttatgagatg cgcaaacgga ttggcatggt
    20761 ttttcaacgt cctaatccgt tcagcaagtc gatttatgac aatattacat ttgctttgaa
    20821 acagcacggc gaaaaaaaca aaaagaaatt agatgaaatc gttgaaacaa gcttgaaaca
    20881 agctgccttg tgggaccaag taaaagacag cttggacaaa agtgccttgg ctttatctgg
    20941 cgggcaacag caacgtttat gtattgctcg tgcgatcgcg atgaaacctg atatcttgct
    21001 gctagacgag ccggcaagtg ctttggaccc tatttcgaca ggaacggttg aagaaacact
    21061 tgtaaacttg aaagaagact atacgatcat catcgtgacg cataacatgc aacaagcagc
    21121 tcgtatcagc gactatactg cctttttcta tttaggaaaa gtaatggaat atgacaaaac
    21181 taacaagata tttaccagac caaaaattca ggcaaccgaa gattatgtgt ccggtcattt
    21241 tggttaagac ggaggcctca acatgacaaa agagattatt tcatctaaag atcttcatct
    21301 gtattatgga aagaaagaag cactaaaagg gatcgatcta agcttcaatc aaggggaaat
    21361 cactgcgatg atcggtcctt ctggttgcgg aaaatccact tatctgcgtt ctttgaaccg
    21421 gatgaatgac ctgattccga atgtaacgat caccggaagt gttttatata aagacaaaga
    21481 catctatggt ccaaaaacag atgttgttga actaagaaaa gaaatcggca tggtattcca
    21541 gcagccaaac ccatttcctt tttctatcta tgaaaatgtc atctatggat tgaaattaaa
    21601 aggggaaaaa gataaaaaag tccttgatca agtagttgaa gaaagcctga aggcagcgtc
    21661 tgtttgggat gatgtgaaag acaaattgca taaaagtgct ttatctttat ccggtgggca
    21721 gcagcagcgt gtatgtatcg cacgtgtttt ggcagttaat ccggaaatca tcttattgga
    21781 tgaaccaaca agtgcgcttg atcctgtttc tactgggaaa atcgaaagta tgctgttaga
    21841 attaagagag cggtatacga tgatcatcgt gacccataat atgtctcaag cttcacgtat
    21901 ttcagataag acggcattct ttttagatgg tcacttgatc gaatacaatg atacgaagaa
    21961 gatattcatg aatccagaaa aacaagagac agaagattat atttcaggtc gatttggata
    22021 aggaggaaac ggaatgctac gtacacaatt tgaagaagaa ttgctgaatc tccataatca
    22081 gttctacgaa atggggatga tggtgagcag cgccgtgcac aagtcagttc gtgcatacat
    22141 caaacacgat aaaatcttag cccaagaagt tatcgaaaac gatataaata tcaataacat
    22201 ggaaacacgc ttggagaaga aaagttttga aatgatcgca ttgcaacagc cagttacaac
    22261 tgatttgcgt atgatcatta cggtcatgaa agctagctcg gatttagaac gtatggccga
    22321 tcatgcggtg tctgttgcta aatccacgat tcgcgtaaaa gggcagacga gaataccgga
    22381 aatcgaaaaa gagatctccg atatgtccga ttatgtgaaa aaaatggtgg acaacgtcct
    22441 agttgcttat gtcaaaacag acgaaaaaga cgcacgtacg atcgccaata tggatcaaag
    22501 agtcaatgac tatttcaatc gaatctataa tgcaacaatc aaaaatatgc aggaaaatcc
    22561 tgagacagtc atcagtggaa cagactactt gaatgttgcc agttatctag aacggatcgg
    22621 agactatgtg acaaacatct gtgaatggat cgtctacttg gctacaggga aaatcactga
    22681 gttaaatacg aaccacaatg agattttcta aactgaagct gtgattatgc cgtttgttta
    22741 ggaaaagagg ttgtgaaatc agcggttgtg aaaaagctgt tctgcatcaa gtaataagaa
    22801 cagtcaaaca aaaaatagct cctcatattt ttcgtttggc tggacttatt accgcagatg
    22861 caagcttttg aacaccgttt attcgggaaa gggtttgtga caaaacatgt cacaaaccct
    22921 tttcatcatt tcatagaaag gataaagaat gcaaaaaaaa attagcatca tcactatatt
    22981 ttttatttgt gtgcttactt catgggttat tcagcatcaa gccgctttat atgaagtacc
    23041 tgtcatcgag atagaaaaag tacgaataaa agaggatcat caattgatta caggaaaact
    23101 gatgaacacg gatcaaaaag gagaatcgat ttatttatcg gttccttatc aaaaaaatca
    23161 aatagaaaca gcaaatataa aagcgagaca aatgtatcta gtccaaatgc atggagatga
    23221 atggcagcta gtagcaaaaa aaaatgatgg ctggcttttc ttcttcacaa gtatcttcat
    23281 tggcgccatg ttgcttattg gtggaaaaca gggtctattt gctttaggaa gtctcatcat
    23341 caatatcgtt ttacttttac ttttgttgct catctttact aaaacagaag ggatttctct
    23401 attaaatata tctatcctgt tcgtcgttat agggatgatt attagcatat tggctttaga
    23461 tggatggaat agaagcagta taatcaaaat ttcagctgca gttacgagtg tttttttagc
    23521 attttttatt tgtcttttga caatgaatca ttggaaagac caaggactgc gctatgagga
    23581 actgaattat ctgacacgac cgtatcgttc tgtttttctt tctagcgtac tgattggaac
    23641 agctggagga acactcgata ccgttatcac agtcattgcg acattagaag aattgactaa
    23701 gcgacaacca gaaataacag cgaaacagtt atggctttca ggaaggcaag tgggtcgaga
    23761 tgtgattggc tcgatgacca atatcctttt gttcgtctat atcagtgggt cagttccatc
    23821 gcttctttta tatttaggaa atcaatggtc gttcaaagaa acgatagaac agcatttatc
    23881 attggaattt ttacgcgtca ttgctggcag cttaggaata gtattatcga tccctatctc
    23941 ggtcctcttt tttctgactg taaggaggct gaaacgatga atgtacttat tatacttgtt
    24001 gggattttag caatcagcgt gctacttatt ggcggtacgc aaggtatgta tattctttta
    24061 ggcttattca tcaatttagg gatctttttc ctgttgttat tcggttatca tcagaagtgg
    24121 ccgatactgg cattgtcggt cataggattt ttattgattg ctgtggttat cttatttttt
    24181 atcaacggtt ataatctaaa aatgcgtgca gcttttgtga gtatactgat ctttttattt
    24241 tgtttcctac tattgcttcc tatcactgat tttttagcaa tccaaggttt tacatcggta
    24301 gaactagaag aattatctgg cttagataag actttagcga ttgattttag attacttacg
    24361 agatcgttgc ttctgattag tcttagtggt gctgttttag atgcgagtgt agcaatttct
    24421 agtgggacat tcgaagtata tcaggcaaat ccgcaattat ctttttacca attgcgccat
    24481 tctagtttcg ctattgctaa aaaaatattg gcgtcaacag taatgacact tctttttgca
    24541 ttcatgggaa gttctttagc gttgatcctc tggtttattg atttagacat tccttttcag
    24601 caagtgatca atgaaaaatc atttgttcta gaatatacga tggctattct taccacattg
    24661 tccgcactac ttgttcttcc tttaacctgc gtgactacag catatctctt tacaaaaaag
    24721 aaagaacaac tcaagatgga gtaaaagtta caacttaagt cctatgacat ttttactgaa
    24781 tcagtgcata atatggatgt aaggaaaagc ttgcaaacaa ttttgcttaa ggaggcaatt
    24841 tccatgaaag aaagagaacg tattttagat ttagtgaaaa aaggaatcct ttcgactgaa
    24901 gaaggcttag atttattaga aagcatggca actgaaaaag acgaaaagca aatcaaaaaa
    24961 gaagcagaca aggtaactgc tagtcataaa gaaaaagatc aggcaagtca attgatcgat
    25021 aaacttgaaa acggcgaaga agaaatttct gagccagttg atccaaagga aagagaaaga
    25081 caagatcaag aaaacttaga aaagatctta gatgaactag caactgaagc gaataaaact
    25141 tcggcgcgtc tagatgaagt aaatgctcaa ttcgctgaca ataaggcagc acgcaatgag
    25201 aaacaggaag cattgatgca gctgaataca aaagaagaat taggcgagtt gacagaagaa
    25261 gaattagcac agcgtcagac gttagaagca gaaatcaaag aattagaagc tagtggcgat
    25321 acactgttag aagaacaatt gaaattagaa gcagaattaa aagatatccg taagaatcaa
    25381 tggtctgaga aaaaagaaac gtttactgat aagtttgaat tgccagatga ttggaaagat
    25441 caagcaacag atacactgaa tcaagtaggc gaaaaaatgt cagaagctgg atcacagtta
    25501 ggcaaattct tgaagaaaac attccaaact gtttcagaaa cagtgaatga caacatggaa
    25561 tggaaagatg tcagcttacg tgtccctggg attgcgacaa cgaaatttga gcatgaattt
    25621 tactatgaag ctcctgcagc aagtatcctt gatatcaagg cagcaaacgg aaacgtgacg
    25681 ttaaaaacgt gggacagcaa cgatgtgaaa gttgaagcta agatcaaact ttacggaaaa
    25741 atgggtgctg aaccatttga agcattctct gaacggagcc agattgaggt taatgaagat
    25801 catatctcct tccaaattcc aaataaacgt gtgcgtgcag accttgtttt ctacttacct
    25861 aaacgagtat acgaccacgc agctattaaa cttctaaatg gcaacatcat gattgaaaca
    25921 ttggaagcta aagatattta tacaaaatct acgaatggta acatcatcgt ggaccaatta
    25981 actgcaacga tgctggaagt agaaggtgta aacggcaata tcgatattcg aaacgggaac
    26041 atcttagatt ctattatcga aacagtaaat ggtacagtga catttggtgc gacagcagaa
    26101 aacctttctg tgtctttggt aaatggtgat gtacgtctta cgattaaaga agataatttg
    26161 aaaaaagtag aagcaagttc agtgaacgga aatgtcaaag tcgctttacc agatggcatc
    26221 ggtttggaag gacatgctaa aacaagtctt ggaagtatca acagccgtct gtccaattac
    26281 gaagtagttc gtgagaaaaa agaacggaca aaccaaatgt tgcaattccg tcgtgtttca
    26341 gatggtgaaa tcgcgcaagt gcaactttca acgacaacag gaagtatcta tttaaaagat
    26401 acagataaat aaagaggttg tgaaagagat gttcagcctt gagcaataag aagcgatttt
    26461 gtaaaatagc ttcccctatt ttacaaaatt gtgccttatt gccgatagtc tatctcttga
    26521 acaccgttta ccaagagatt atgaacctgc caattatcgc cgagtcacaa ggaataattt
    26581 cagaaaaagc tcgtcacgtt ttatgaatat ggtaatttgt gaccgagaat tttgcagaaa
    26641 gacaccgatt aagaagaagg ttgtcacaac gattttcttc atttaaaata gtagaacaga
    26701 ggtgagacat gatgaaaaaa aagttgacaa aatctaataa aaatgttgtt ttgacaggga
    26761 ctttagcagg aataggagaa tacatcggga ttgatccgac tgttatccgt gtgctttatg
    26821 tattcctgag cttagttgct tttggaagtc caattatttt atacatttta ttagctttga
    26881 ttatcccatc tgcgcgttct gcacgaacaa actatggaca taacaatgca tattatgaaa
    26941 aaaacaatta tcgtgaacaa actcgtgaac aaacaaaaaa acgcaaagaa gcggaaaaaa
    27001 tcgatgatga cgaatggagt gacttttaat gtcgtacttt caacgtattt tagttaatac
    27061 gttaacattc atatcattgg ctgttctttt tcctgcgatg gtccatgtga gcagtatttg
    27121 gacggccgtt cttgccagct tcgtattgtc tgtgttgaat atgtttgtca aaccgatttt
    27181 gacgatactg tccctgccct ttacattagt cacattggga ttgtttacgt ttgttatcaa
    27241 tgcagcgatt cttaaaatga cctccttttt tgtgggagca gaacatttta gtttttcttc
    27301 attcggtgca gcgttgttag tagctatctt gatgtcgctt gtcaatgtaa ttgtcagcga
    27361 acataacagt agaaaataaa agaaagctgt gccgaactat cggcgcagtt tttttattta
    27421 gaatcaggaa tgcgttttct attacattgg taattaaata aaaacgtttt ttttaattgg
    27481 tagtactttt ggctagacaa tgataaaatg atagagaaca ctctaataga aatgaggtag
    27541 catgaatgtc agaagttgta aaagttagcc aattagtgaa aaaattatct ttggaaatcg
    27601 tgacaggcga cgagaaaagc ttagaccgtg tcatcgtaac tggggacatc tcacgcccag
    27661 gactagagtt gactggttat tttaattatt attcccataa tcgtttacag ctatttggca
    27721 gtaaagaaat cagttttgcg gaacggatga tcccagaaga gcgtttgatc atcatgagaa
    27781 gattatgtag tgaagatatg cccgcattta ttatttcaag aggattacca gctccagaag
    27841 aaatgatcca ggcagcaaat gaaaatggga tagctgtact gcgttctccc atctcaactt
    27901 ctcgtttatt aggagaatta tccagttatt tagatggtcg ccttgcccct cgaacaagtg
    27961 tccacggtgt tttagtggat gtttacggat taggtgtact gattcaaggt gacagtggga
    28021 taggaaaaag tgaaacagcg ttggagttga tcaaacgcgg ccatcgattg atcgcagacg
    28081 atcgagtaga tgtttatcag caagatgaat taacggtgat cggagaaccg cctaaaatcc
    28141 tggaacatct gattgaaatc cgaggaatcg ggatcattga tgtaatgaat ctttttggtg
    28201 cgagtgcggt acgtggctct atgcaagtcc agttggctgt ttatctggaa gcatgggcaa
    28261 aagataagaa atacgaccgt ctgggctctg aagacacgac cgttgaaatc gcggaagtgg
    28321 gcattccaca aataaaaatc cctgtcaaaa caggacgaaa cgttgcaatc atcattgaag
    28381 tagccgcgat gaatttccgt gcacgtacaa tgggctttga cgcaacgaaa acatttgaag
    28441 aaagattaag tcgcttgatt gaagaaaact caggcaacga ctagcaagga gtgacaatat
    28501 gttagcacaa atcaatcgag ttgcttttga gcttgtaggg attcctatct actggtatgc
    28561 attgatcatc gtatcaggta tcattatcgc tatgtggctt agcagcagag aggctgttcg
    28621 ggtaggatta aaagcagatg atgtgacaga ttttatgttg tggggattgc ctatctccat
    28681 tattggcgct cgcttatatt atgttttatt tgatcttccc cagtatatag ctgatccaat
    28741 ccaaatattc aatatccgtt caggaggctt agctatttat ggcggactga ttgcaggcgc
    28801 gatcacgctg tattttttta ctaaatatca ttttatttct ttgtggactt ttttagatat
    28861 cgctgcacca agtgtattat tagcacaggc aatcggtcgt tggggaaatt tcatgaacca
    28921 cgaagcatat ggaccagaca cgacgcgaag cttcttagaa agtctgcatt taccgcaatt
    28981 catcattgat aatatgtata tcgaagggat ctatcgtcaa ccaacatttc tttatgaatc
    29041 actttggagt ttgattggat ttattttgct tttgttttta cgaaagaaac cacacctttt
    29101 gaaaaaaggc gaagtccttt tatgttatct aatgtggtat agtttcgggc gtttctttat
    29161 tgaaggatta cgtacagaca gtctttactt tttaggtgtg atccgtattt ctcaattatt
    29221 atctgctgtg ttatttgtcg gtacattgat tctatttatt tggcgcagaa aaaaaggaaa
    29281 tttaccggat tatgaccgct cattaggaaa aaatcaacaa attatctgat ttttagagcg
    29341 ttaaaaatga aaaaaatttc aggagtggta aaatgaatta tagagtagcg gttttaggac
    29401 caggctcttg gggtactgct ttggcacaag tcctcgccga aaacggacat gacgttcgga
    29461 tttggggaca tcatgcttca caagtagaag aaatcaacac ccaacacacg aatcaacgtt
    29521 atttaccaga tcattatcta cccgattcca tccaagcatt caccaatatg gaagatgcag
    29581 taaaagacgc agatgcggtg ctattcgtca ttccaacgaa agcaatacgt tctgttgcaa
    29641 aagaactagt gcctaaattg aatacaaagc cagtgatcat ccatgcaagt aaaggattag
    29701 aacagggaac acataaaagg atctctgaca tcttgttaga ggaaatccca gcagaaaaaa
    29761 gaaaagctgt tgttgttctt tctggaccaa gccatgcaga agaagtagca aaacatgaca
    29821 tcactacgat cacagctgca agtacagatg caaaagcagc aaaatatgta caagagctgt
    29881 tcatgaataa ttattttcgg atctatacca atcctgatgt gatcggtgta gagatggggg
    29941 cagctttgaa aaatattatt gctattggtg ctggcgctat tcacggctta gggtttggcg
    30001 ataatgcaaa ggcagcaatc atgacgcgcg gattagctga gatcagccgt ttaggtgtag
    30061 caatgggtgc caacccgctg acctttatcg gattaagcgg aataggcgat ctagtcgtta
    30121 cttgcacaag tgttcattct aggaactggc gcgcaggcaa cttgttagga caagggcagc
    30181 ccctagaaga ggtgctagcc aatatgggaa tggtcgttga aggtgtgaat actacccaag
    30241 cagcgatgga actagcccaa atgatggaaa tcgaaatgcc catcactcaa acaatctatg
    30301 aagtgctgta tgaacaaaaa gaaatcaaaa cagccgcaaa agaaatcatg ttacgtgatg
    30361 gaaaaaaaga aaatgaattc tcattaacaa ttttttgaag gagcgaatgt atatgaaagt
    30421 taaaaaagca gttattccag cagcaggatt aggaacaaga tttttaccag caacaaaagc
    30481 tatggcaaag gaaatgttgc caattgttga taaaccaaca atccagttca tcgttgaaga
    30541 agcgttgaaa tcaggtatcg aagacatctt gatcgttaca ggaaaagcta aacgaccaat
    30601 cgaagatcac tttgattcaa atattgaatt agaaacaaac ctaagtgaaa aaggaaagac
    30661 cgaattattg aaattagtag aagaaacgac agatgtcaac ttgcatttca tccgtcaatc
    30721 tcatccaaag ggtttaggac atgccgttct tcaagcaaga gcctttattg gaaatgaacc
    30781 atttgtcgtt atgctaggcg atgatataat ggaagacgaa gtaccattga cacagcaatt
    30841 gatcgatgac tatgagcgta cacacgcttc tacgattgcc gttatgcaag ttcctcacga
    30901 tgaaacttca aaatacggaa tcatcaaccc aggggaagta ctggaagatg ggttatacaa
    30961 tgtcaaaaac tttgttgaaa aaccagatcc aagcaaagca ccaagtgatt tagctattat
    31021 tgggcgttac ttattgactc cagaaatctt tgatgtatta tcagaacaac aaccaggtgc
    31081 tggaaatgag atccaactaa ctgacgcaat cgatacgttg aataaaacac aacgcgtatt
    31141 tgcccgtaaa ttcacaggta aacgttatga cgttggtgac aaatttggtt ttatgaaaac
    31201 aagtattgaa tatggtctga cacatccaga agtaggggca cctttgcgtg aatacatcat
    31261 gtctttaggt aaaacattag aagcagaagg aagcaagatc aatcataatc caaaaggaat
    31321 aacagaaatc aaagaagaaa agaaaaaata atcttttttt ctaacgagct tcagctgcta
    31381 attgaataaa aagtagttga agctcgtttt tatttgttta aaaaacacga aaatgctatc
    31441 cttatagaaa agagagacaa gaagggagtt tgatcatgtc aggaggagaa attgcaggat
    31501 tgatcgcagc aatcgccttt gcgatacttg tcgttttcat cgttcgcgta ttgttgcaag
    31561 tttccaaaac tttagaaaaa gtagaccaga ctgtcgcaga agcaaatacg acaattgaga
    31621 tcgtcacaca agacgtagat cttttatcta gacaagtaga aggcttgcta gtcaaaagta
    31681 atgagctgct cttggatatc aatggaaaag tcgcaacgat cgatccattg ttcacagcag
    31741 ttgctgattt aagtgaaagt gtatcggaac ttaatcattc tagtaaaaac atcgtgacga
    31801 aagtcggcgg aatcggaaaa acgacagcta aagcgactgt tgccggaaaa gtcggtggaa
    31861 cagcgatgaa attttttaaa aacaaaaaac atactgaaac gacaggagga aaataagaat
    31921 ggctaaaaaa ggtggatttt tattaggtgc agtagttgga ggtacagccg cagcaatcgc
    31981 cgcattattg ttagcaccca aatctggtaa agaacttcgg gatgaattgg ctgctcaagc
    32041 agatgatttg atggacatgg cttcagatta cacagatatg gcaaaagaaa aatcatctga
    32101 gttaacaggt attgcaaaag aaaaagcgca aggtctttct caacaagcag gggatctagc
    32161 aggcaatgtt aagcagaagg taagtgaatc agcagaggaa gcaggaagtg aatcagatga
    32221 tatcttgtta aacctcaaag aacaatcagc tgatttatct aatcgtttca aaaaaacagc
    32281 agaagatctg aaaaagccag cagacgaatt aggtagtatt gctaaagata cttcagaaga
    32341 tatcatgatc gatgtaaaag atgctgcaga taaagcagga gagacggtca atgaaggaat
    32401 gtcagaagca aaaccagttg cagatgacac aaaagaaatg ttgaaaatgg caaaagaaga
    32461 tgcaaaagaa gctaaaaaag aaatgaaaga tacaatgaca gattcgacta ccaatcacta
    32521 agattactca tttctagaat aaaaaaggtg agagcggacc agctctcacc ttttttattt
    32581 tcattactct aaaacaagta attcttttgg tgtagtcgtg aatggcacac aaccatcact
    32641 tgtcacatac acacaatctt cgatacgtac ccctactttt tcaggaatat agattcctgg
    32701 ttcaagagag aagcacatcc cttcttcaat cactaagtcg tttccttcta caagagaagg
    32761 gaattcgtgg acggttgttc cgattccgtg acctaaacgg tgattgaaat attcgccgta
    32821 gccatagctg ttgatcacgt ttcgtgcgat ttgatcaagt tcacctgctg tcacacctgg
    32881 gcgaactgcc tcttgtgcgg caagctgtgc ttcaagagtg atgttataga ttttttcttg
    32941 gaactcagtt ggtttttgat aagcaactgt acgagtagca tcactgcaat agccattcca
    33001 aataacgcca agatcgaata acactagttc atttggtgaa accttcgtgc ttcctggtgt
    33061 accatgaggg cttgcagcat ttgctcctgc aaggactaat gtgtcgaaag acatttgtga
    33121 aacaccttgt ttttttaatt ggtattcgat ttctgcgata attgcttgtt cagttacgcc
    33181 ttcttttact gttttgaagc caatttcaaa ggcaacatcg gcccatttac cagcttcgat
    33241 cagtttgtct ttttcttcta ccgttttgat cagttgcaga cgctggataa tagaagttag
    33301 atcggtgtaa gaatctgtac ctgtcaagat atcagagata gcatcgaagc gatcgacagt
    33361 cagagcattc ttttcaatac ctacattacc aggttgccca tagcgtgcgc gaagctcttt
    33421 tccgatgatc tcccaaggat tttcgctgtc taagtagcca cgtacatcgt acgcccagct
    33481 gctgttttga gcatcttcaa cttccagagc aggagtgaaa aggaaaggat cttgttccag
    33541 agggataaaa agagctagta cacgctcgtg aggatcactt tcatatccgg aaaagtaagc
    33601 gatgtggcca gggtcgctga tataagccaa ttctacttgg tgttgtgcca tccattgacg
    33661 tagttcattg atttttacgt tattcatttg gcaacctact ttctaaatta attttgtatt
    33721 caataattgt accactgatt gattaaaaga ccaacgttct taaaactgaa aaaagtaaaa
    33781 attttcatga aaacagagat aaaacggttg caattgttga gattatttga tatcctattt
    33841 tagaaatgta agacgtattt tcagaaaaca tataaaatat gaataggaga acacaaatgg
    33901 aaaagcaaac aatcactatc tatgatgtag cgagagaggc aaatgtttcg atggctactg
    33961 tttcacgtgt cgtaaatggc aatccaaacg tcaaaccagc aacaagaaaa aaagtcttgg
    34021 aagttatcga tcgcttggat tatcgtccaa atgcagtcgc acgtggcttg gcaagtaaga
    34081 aaacaacaac agttggggta atcattcctg atgtcagcaa tatgttcttt gcctctttag
    34141 cacgtggtat cgacgatgtg gcaacaatgt acaaatacaa tatcatctta gcaaactcag
    34201 atggcaacga tcaaaaagaa gtcaatgtat tgaataattt attggctaag caagtggacg
    34261 gcgtgatctt tatgggtcac catatcacag acgaaatccg tggagaattt tcacgttcaa
    34321 aaacgcctgt tgtactagct ggttccattg atccagatga acaagtaggt agcgtaaaca
    34381 ttgattatgt gtcagcaaca aaagatgcag taaataaatt agcaaaaagc ggcaataaaa
    34441 aaatcgcttt tgtcagcggt gcgctgattg atcctatcaa tggtcaaaac cgtctaaaag
    34501 gctataaaga agcattaagc gaaaacaatc taccatatag cgaaggattg atttttgagg
    34561 cacaatacaa ctttaaagat ggtctgtctc tagttgaccg gatccataat agcggtgcaa
    34621 cagctgtcta tgtttcagac gatgaattag ccattggtat cttggatggt ttattagacc
    34681 gcggcgtaaa agtaccagaa gaattcgaaa tcatcacaag caacaattct ttactgacag
    34741 aagtagctcg tcctcgtttg acaagtatca cacaaccatt atacgattta ggagcggtat
    34801 ccatgcgcct attgacgaaa atgatgaata aagaagaagt cgaagaaaaa acgattatct
    34861 tgccttatgg aatcgaagaa aaaggatcaa ctaaataata aaagatcaat catttaaagg
    34921 ttgttccaaa agtggtcaac ttctggaaca acgtttatac gagaaagggg gttatgacaa
    34981 aattttgtca taaccccttt ttttttgtga ctttaaaaaa agagaaaatg gcggaaacaa
    35041 accttacgtc catcgtatac gacacaatct gtgcgtagat gctacatttt gttcactact
    35101 atcaaaatta ctttaaaatg aaaacgtgat aaaaaagaaa gtagaaaagt agataagtaa
    35161 ctctaaaaaa tccaaaaaaa tccaggctcg attgcgtact tatcttaaag gagaaacaat
    35221 ccatcatata taacccgaaa ggagacttct atttgcctaa gaaaagacga aagaccatag
    35281 tcatcatttt gaccattctt actataggaa tcggagggaa attttacatg gacaaaagag
    35341 aaatgcaaag acagaaagat ttactcgcag tagaaaaaca gtccgtcaaa gttctgaaaa
    35401 atacctttgc ggatatcaag gaagtgaaga ttgaaaaatc tgctcgaaat gaaatgacgg
    35461 gttcataccg gatagttatt ctaatgacta ataaacaaga tcaatcgatt tatttttctt
    35521 atagtttttg gaaagaaaga aatgagatag gaagttacgg aatagttgat gaaaaaaagc
    35581 aaaaagaagg aaatacgctc aataaagtta aagtaactta ctctaatgga aatgaggaaa
    35641 gtatttaatg attactgaca aacaatatag tgaattaagt gatgctgttt actggctaga
    35701 tcctaaacat agagactatg tacctgaaat gcaagaaaat ttaagtttta aaattaacgg
    35761 cactatgtat aaaatcctaa aaatcaaaaa ctccttcgac ggcatgcagg cgatggctgt
    35821 agcgccaatc gtccatagca aattggagaa aaatttcaaa aataaaaaaa ttcctgcaaa
    35881 ctttcgcgta cttaaataat gaaggtgtca atacttacat aaaaagaaag gaggcacgtt
    35941 cttgtcaagg aaaataagaa aactcagctt agccatttta ttaatcgtca tattagggat
    36001 cggagggaaa gtctatatgg acaaaagaga agcacaaagg cagaaagatt tactcgcagt
    36061 agaaaaacag tcagtcaaag ttctgaaaaa tacctttgcg gatatcaggg aagtgaaggt
    36121 agaagaattt aaaaaaaacc ctgttaccgg aagttatggt gcacttgtga caatgacaaa
    36181 taatgaaggg aaatctgttt attttgatta tagtttttgg aaggagagaa atgaacttgg
    36241 tggttttggc attgagaaca gagcggtaca aaagacagga gtcactataa aaagagtaaa
    36301 agtaattttt tctaacggac aggaggagtt agtatgacaa taaacgataa gcattacaat
    36361 gatattagtg agaaggttta ttggctagat cccaaatatc cccgctacaa tgagggatac
    36421 aaaaagaact ctgttaaaga atttgctggt atggaatttc aaatcctaca aatcaaagac
    36481 tcattggacg gcatgcaggc gatgtctgta gcgccaatca agaacggtaa agtggatctt
    36541 tctcaagtag tgattgcttt tgcaggaacg aacttaagtg attggaaaga tttggaaacg
    36601 gatgcaagaa gtatcatgaa tctctacggg aatagaccag gagcaagttt ttctgaaaaa
    36661 gcaacaacaa acaagatttt atccgttagt ggattgaaaa cagcggggca aatcaattca
    36721 gcgattgcat ttgctaatga agtaaaaaag gaatatggag aagctgctat ttctactacc
    36781 ggccattcat taggtggatt cttagcatta tatgtagcag cagagaaaca atggaaaaat
    36841 gtcggcttca acgggccgga ctcgtatgat ctcttatcac cagaagcaaa aaaatgggtg
    36901 gaagaaaatc ccggtatgct caccaattac cgaaatcacg ccgattttat cggtaatttt
    36961 ggtggtaatg gcacaggtgc tgaagttcgg atcagtatgg aaacagcatt cttgaatctg
    37021 cttgcttccc acagtttaac ctcttggaag tttgatgaaa atggaaagct gattattcct
    37081 gaaaatgaaa acaatgttaa agctcgaaaa caacagaaag aaaaccaagc gacccagttg
    37141 ttttcctatc agttgcaaga actgaacaac atacgtaaaa aactacgatt aagtggcggc
    37201 cagctgacag ttaatgaaca aatttactta gatgacacac aagctaggtt agtagtggaa
    37261 aagttatcgg caaaaatgaa aagcgctatg gaagaagtta tccgcatcaa tcaaagagga
    37321 atttttgagc tagagaaaaa atggcaagaa ggcttgcggc aagcacgtgt tgtagcccct
    37381 tcgttgactg aacaggaaat catagaagca ttagctagtg tgggcgccac aaaagaaaac
    37441 ttagtcgatt ctgcgaaaga agtctatctg ctacgaatca ataaggcaag acgtatgggg
    37501 gaggcctttg atacattggc gaaggagatc caagacaaaa tcaatgaaat cgtggcacga
    37561 gatcgagaat tagcacagca attcaattaa aggagaagta gatgaaacta gaagaattgg
    37621 aaagagatta tcgaagtgaa cgaagacaat tagaagctca agaagatgaa ttgatccact
    37681 ttaagaatca aggattacga ttgatggagc aagaggagca gcgactgcat catgtattaa
    37741 agaacttagc tttagataat gagcctctgt ttcaagcaag acgaagttat tcgaattatc
    37801 aggagcatta tcaacaagcc ctacaagtag aacgacgaaa actctatcag cagcaagatg
    37861 aactagaacg ttcctatcgt caaaaaatca gacaaataac tatggagaaa tcatcttatg
    37921 aatgaatttt taatgcaatt actaaaagaa ttagagaaaa atctacatcg atcaaatgta
    37981 cgttcagttg gtatggggat atctcagcaa atttgggcag aaaaacgatt gacagatcaa
    38041 aactaccaaa tgactgcaga agaagctgct caagaatttt ctaaccgaca tgtacacttg
    38101 aaaggatcag taaaagggca atttgcagaa aaactaactg aattattcca aagacaagaa
    38161 aagaaattaa aacagtttcg ttagtaaagt tatatagaaa aattttgtga aaatggttt
//
LOCUS       DACDIG010000024        36269 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.024, whole genome shotgun sequence.
ACCESSION   DACDIG010000024 DACDIG010000000
VERSION     DACDIG010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.024"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            356..631
                     /locus_tag="IV247_001250"
     CDS             356..631
                     /locus_tag="IV247_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777329.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            644..2722
                     /locus_tag="IV247_001251"
     CDS             644..2722
                     /locus_tag="IV247_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP8777330.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            2882..4768
                     /locus_tag="IV247_001252"
     CDS             2882..4768
                     /locus_tag="IV247_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8777331.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4780..5727
                     /locus_tag="IV247_001253"
     CDS             4780..5727
                     /locus_tag="IV247_001253"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP8777332.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5747..6274
                     /locus_tag="IV247_001254"
     CDS             5747..6274
                     /locus_tag="IV247_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8777333.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6337..6990)
                     /locus_tag="IV247_001255"
     CDS             complement(6337..6990)
                     /locus_tag="IV247_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP8777334.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7122..7964
                     /locus_tag="IV247_001256"
     CDS             7122..7964
                     /locus_tag="IV247_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8777335.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8122..9018
                     /locus_tag="IV247_001257"
     CDS             8122..9018
                     /locus_tag="IV247_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP8777336.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            9021..9734
                     /locus_tag="IV247_001258"
     CDS             9021..9734
                     /locus_tag="IV247_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP8777337.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9754..10272
                     /gene="msrA"
                     /locus_tag="IV247_001259"
     CDS             9754..10272
                     /gene="msrA"
                     /locus_tag="IV247_001259"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP8777338.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQDYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10269..10496
                     /locus_tag="IV247_001260"
     CDS             10269..10496
                     /locus_tag="IV247_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAP8777339.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10648..11199
                     /gene="lepB"
                     /locus_tag="IV247_001261"
     CDS             10648..11199
                     /gene="lepB"
                     /locus_tag="IV247_001261"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP8777340.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11282..11824)
                     /locus_tag="IV247_001262"
     CDS             complement(11282..11824)
                     /locus_tag="IV247_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP8777341.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(12061..12459)
                     /locus_tag="IV247_001263"
     CDS             complement(12061..12459)
                     /locus_tag="IV247_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP8777342.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12719..13579
                     /gene="ylqF"
                     /locus_tag="IV247_001264"
     CDS             12719..13579
                     /gene="ylqF"
                     /locus_tag="IV247_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAP8777343.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13572..14339
                     /locus_tag="IV247_001265"
     CDS             13572..14339
                     /locus_tag="IV247_001265"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAP8777344.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14395..15252
                     /gene="dprA"
                     /locus_tag="IV247_001266"
     CDS             14395..15252
                     /gene="dprA"
                     /locus_tag="IV247_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAP8777345.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15374..17452
                     /gene="topA"
                     /locus_tag="IV247_001267"
     CDS             15374..17452
                     /gene="topA"
                     /locus_tag="IV247_001267"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAP8777346.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17421..18797
                     /gene="trmFO"
                     /locus_tag="IV247_001268"
     CDS             17421..18797
                     /gene="trmFO"
                     /locus_tag="IV247_001268"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAP8777347.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            18995..19900
                     /gene="xerC"
                     /locus_tag="IV247_001269"
     CDS             18995..19900
                     /gene="xerC"
                     /locus_tag="IV247_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAP8777348.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            19937..20485
                     /gene="hslV"
                     /locus_tag="IV247_001270"
     CDS             19937..20485
                     /gene="hslV"
                     /locus_tag="IV247_001270"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAP8777349.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            20499..21899
                     /gene="hslU"
                     /locus_tag="IV247_001271"
     CDS             20499..21899
                     /gene="hslU"
                     /locus_tag="IV247_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAP8777350.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            21917..22708
                     /gene="codY"
                     /locus_tag="IV247_001272"
     CDS             21917..22708
                     /gene="codY"
                     /locus_tag="IV247_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAP8777351.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            22790..23665
                     /locus_tag="IV247_001273"
     CDS             22790..23665
                     /locus_tag="IV247_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP8777352.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(23797..24435)
                     /gene="plsY"
                     /locus_tag="IV247_001274"
     CDS             complement(23797..24435)
                     /gene="plsY"
                     /locus_tag="IV247_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAP8777353.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            24562..24996
                     /locus_tag="IV247_001275"
     CDS             24562..24996
                     /locus_tag="IV247_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAP8777354.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            25162..27207
                     /gene="parE"
                     /locus_tag="IV247_001276"
     CDS             25162..27207
                     /gene="parE"
                     /locus_tag="IV247_001276"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAP8777355.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            27219..29669
                     /gene="parC"
                     /locus_tag="IV247_001277"
     CDS             27219..29669
                     /gene="parC"
                     /locus_tag="IV247_001277"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAP8777356.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDRSIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            29946..32177
                     /gene="pflB"
                     /locus_tag="IV247_001278"
     CDS             29946..32177
                     /gene="pflB"
                     /locus_tag="IV247_001278"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAP8777357.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            32421..33182
                     /gene="pflA"
                     /locus_tag="IV247_001279"
     CDS             32421..33182
                     /gene="pflA"
                     /locus_tag="IV247_001279"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAP8777358.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            33267..34193
                     /locus_tag="IV247_001280"
     CDS             33267..34193
                     /locus_tag="IV247_001280"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAP8777359.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            34375..35268
                     /locus_tag="IV247_001281"
     CDS             34375..35268
                     /locus_tag="IV247_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAP8777360.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            35402..35527
                     /locus_tag="IV247_001282"
     CDS             35402..35527
                     /locus_tag="IV247_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8777361.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(35661..>36269)
                     /locus_tag="IV247_001283"
     CDS             complement(35661..>36269)
                     /locus_tag="IV247_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777362.1"
                     /translation="NFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWLLLDHSDIT
                     EAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSFLAILFYSF
                     NHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSLFSFFVLAL
                     MPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
BASE COUNT        12725 a         6218 c         7625 g         9701 t
ORIGIN      
        1 ttaatcatac tagagaacga tatgtttttt aatacctaaa ataaaaatgg ggctgaagaa
       61 tcaattctga ttcatcagtc ccataaatta tagagccgat ttttgtcgca gccccatttt
      121 tttgttgtga taaaagaaca agaaaggcag gaatgggaaa gtaaacatcc ttttgtcaga
      181 aacttttatc tttcttaagt tttacttgat atctgatact atttggaata gaatgaggcg
      241 ttgaaaaatt tatggaaatt tctaactagc aataaacgat gaaacaatga aatatttttc
      301 atccaaaaag aactttgcta taatgatgga agtttgtgta taggagatct ggaaaatgag
      361 agagagtttt gaacaacaaa aaaaactgtt gcatgatcgc tatggcgccc tttcgatgga
      421 tgaccgccgt caaatacttt gtaagttgcg taaaagaaat atattgatgt atcgtcagtt
      481 ggaacgtctg aaacatgatc tgcttcgttt ggagtcaaaa cgtgtacagt gtgaattaga
      541 ggggaaccaa acacaggttg aagtagtcga aaccaaaatt ttaaagaaaa aagaacaatt
      601 tttaaaaatg cttacacaaa ataaaaaata aaggggatgg acgatggaat ttgctgagtt
      661 agttattgta tttgcagtaa cgatcacctt atcgaacata gctagccgca ttttgccaat
      721 gattccagct ccactgatcc aaattttcct tggagtggta ttaggtctga cagagtgggg
      781 acaatcaatc gattttgaac ctgaattgtt cttagttatg attattgcac cattgttatt
      841 tagagaagga gaaaaagcag atatttcttc gattctcaaa aatttcggga caattttgtt
      901 cttggcattt ggaggagtca ttttgaccct tgtcggggta ggtgcgacgt tatcattcct
      961 gttgccaagt gtcccacttg ctgcctgctt tgcttttgga gcagcgttag gtccgacaga
     1021 tgcggtagca gtcagttctc tttctggacg agtgaatatc cctaaaaagg cgatgcatat
     1081 tttggaagga gaaggtcttt tgaatgatgc atctggtgtt actgccttcc agtttgctct
     1141 tggtgcgctg atcaccggat ctttttcagc agtaaacgcg gggatgagtc tagtagtttc
     1201 tagtattggg ggagctttga tcggcttttt gcttgtctgg ttcaaacaaa aaataattca
     1261 tttgatcgaa aaagcatcag cacaagatgt cacagggtat ctgctgatcg aacttttgct
     1321 tccttttcta gcctatgtat tggcagaggc atttgatgta tctggaatca ttgctgcagt
     1381 ggcagcaggt attctccaag cttctggttt taggaaaatc tctgtgtttg atgcagaatt
     1441 gtcgagcttg tcccacagta catggacaac tattgctttt acactgaatg cattagtatt
     1501 tatattttta ggaatcgaat tgacgcaagt gttctcgcca gtatggggag acggtttata
     1561 tccaaacggt cttttattag cgattatcgt attaatctct gtcatgcttt ttgtgatcag
     1621 attcatttct atcagtcttt tttatgtatt caaagatggc agtaaaaagt tcaaaaaaca
     1681 gttgaacgag atcttgatct taacatttgg cggtgtaaag ggaacagtca gtttggctac
     1741 gatttttatt ttgccatttt caatcaataa tatgatgttt taccagcggt ctttgctttt
     1801 gtttctgacg gcgggtgtca tcttagtgac tttggtcata gggatcattg tcttgcctat
     1861 gctgacggaa acagaggaag cgaaaagcac ggatttgaat gctttgatga ttttagaaga
     1921 ggtcgttgaa gttttgcgaa aagaaataaa ggaaattgat cgtaatacaa aagaattttt
     1981 agccactgaa gcagtgatag aaaattatca agaacggata cgtgatctgt acttggaaga
     2041 tttaaccgat gatgaaaagc aagaagtaca agaaatacaa gcactgattt tgtcgatcga
     2101 acgggatgga ttggatgaaa gttatcgttc gggcaagctt tcttctaatg gttatcgttt
     2161 ttactcccgt tttctttctc gattcgaaca ttctatcaca agccagattt tgtcttttat
     2221 cggcttttgg ttcattgttg tccgtaggtt aatgcgaatc gttctccatc cgaaaatgtt
     2281 ttggcaaaga agatatgctg atcgtcagac atttattagt gatgaagata tccaagaaat
     2341 cagagagaca tatcaaaaaa atactcagtt gattattgaa agtttagata acttgactga
     2401 tatttatgat gaaacgatgg ttcaattttt tatccagcaa cgcaaacttg aaggaataaa
     2461 aatgaactcg gggaatttga tttcttcgtg gatgatccag caagatagtc tgtttacgaa
     2521 aaaaatgcta agaggttatt atcttgaaag aaaaatcatt gatgaatacg aagttgctga
     2581 aaaaatcacg acgttttctg cgaacagcta tcgcaggaat atcaatttgt tagaatctta
     2641 tacgatgaac aaaccatctg ataacttttc tttccgattt gcgtttcgag caaagtcaaa
     2701 aaaaacgagt ccaataaaat aaaaaggtaa aaattttcaa gttttataaa aaacaaaaca
     2761 tatcagaaca ggatcgaatc ggtcctgttc tttttttgag aacacaaaaa gaacaaatac
     2821 caaaaaaatt ttcctgaagt tcactatttt tatgatacaa tatccaagat aaggagggaa
     2881 catgttgaaa gaattaaaag taactgacct caaaaaaact tatggtgaaa aagatttgtt
     2941 tgaccagatt tcttttttgg tccacgaaaa agaccgtatc gggttgattg ggacgaacgg
     3001 gacgggtaaa acaagtctct taaatattat tgccggtatc gatagcagcg acggtgatcg
     3061 gcaaacagtg ttttatccaa ctgattaccg tattggttat ctttctcaag cagctgaatt
     3121 ttccgatgaa ttaactgttc tccaagcagt ctttcaagga aacagcccct tgatccaaac
     3181 tgttcgagca tatgaggaag cattgatcga actaggggaa aacggagaag accctgatgt
     3241 gcaaaaacgt tatgctaaag cagaagaaca aatgaataaa gaagatgctt ggactacaga
     3301 taccaacgca aagattattt tgcaaaaatt aggaatcagt gaattagata aaaaaatcag
     3361 tactttatca ggtggtcaga aaaaacgagt tagtctggcg caagtgttga ttgatgaacc
     3421 cgatttacta ttactagacg aaccaacaaa ccatttagat tatgaagcga tcgagtggtt
     3481 ggaaaactat ttgaaacaat accgtggcgc actattgatg gttacccacg atcgttattt
     3541 tctagataga gtagcaaacc gaatcttcga actttcattt ggaaaattat acgagtataa
     3601 aggaaattat gaagcttacg tcttagaaaa agcagaacgt gatcgtgtag aagtagaaca
     3661 agaagaaaag cgcaagcggc tttataaaca ggaattagct tggatgaggg ctggcgtaca
     3721 ggcacgtggc accaagcagc aagcacggat caaccgtttc gaagatctaa aggagaactt
     3781 gtaccaagta aaccaagaag atgatttaga gctgaaccta gcgacacaac gtttagggaa
     3841 aaaagtactg gaaatcaaag atggcagtta ccgtataaat gaccaaacac tgctagaaca
     3901 tttagattta ttgattcaat ctcgggaaag actaggtatt actggtaaaa atggtgcggg
     3961 aaaatcgaca ttgcttaata ttctagctgg cagaattccc ctggatagcg gtacaatgtc
     4021 tatcggtgaa acggttcatc ttgcgtatta tacacaagaa aatgaagaaa tggcaccaga
     4081 taaacggatg attgcttact tacaagaagc tgctgaagaa gcaaaaacag cagatggcag
     4141 tcaaatcgga gtagcagaat tacttgaacg atttttattt ccaagattta tgcatgggac
     4201 actgataggc aaactttcag gcggagaaaa acgccggtta tttttgttga aactgctgat
     4261 tcagcaacct aatgttttat tattggatga accgaccaat gatttggaca tagcaacatt
     4321 gacgattttg gaagattatt ttagaagttt tccaggtgct gtgatcactg tttcccatga
     4381 ccgttatttc ttggacaagg tagcagataa gttattagta ttccaaggaa acggaaaaca
     4441 agaactttat tacggaaaca tgtccagtta tttactgaag caaaaagaga cccagcagcc
     4501 tgctgaaaaa gcgaaaccaa aaatacagag caaggaacct gctgaaaaga agaaattatc
     4561 ttacatggaa caaaaagaat gggaaacgat tgaagatgag attgcagaac ttgaagaaaa
     4621 aatctcattg ctccaagaag agatgaatca tcaaggagat aatttcactc gattacaaga
     4681 attgcaaaac gacgtatctg aaacagaagc acaacttgaa gaaaaaatgg cacggtggga
     4741 atacttgagt gaatgggtag aagattagga ggaaaaagga tggaacaagc gtatcttgac
     4801 ttaggaagaa aattgttgga agaagggcat cgtaaagaag acagaacagg cacaggcaca
     4861 ttaagcttat tcggatacca aatgcggttt gatctgcaaa aagggtttcc tttgttaaca
     4921 acgaaacgag taccttttgg attgatcaaa agcgaattgc tttggttttt aaaaggagat
     4981 acgaatatcc gctatctatt acaaaataaa aaccatattt gggatgaatg ggcatttgaa
     5041 cgctacatca aaagtccaga ctataatgga ccagatatga ctgattttgg ccatagatgt
     5101 ttgacagatc ctgaattcaa tgaagaatat aaaaagcaga gcaagctttt ttgcgagaag
     5161 atcctaaccg atgattcctt tgctgaaaca tacggcgatc ttggacacat ctacggctat
     5221 caatggcgtc actgggaaac aaaagacggt ggctttatcg atcaaatcaa acaagtgatc
     5281 gaagcaatca aaaagactcc tgattctaga agattgatcg tttctgcatg gaatccggaa
     5341 gatgttccta gcatggcttt gcctccttgt catacgatgt ttcaatttta tgttcaagag
     5401 ggacggctaa gctgccaatt gtatcaacgc agtggggatg tgtttctagg cgttccgttc
     5461 aacattgcaa gttatgcgtt gctgactcac ctaattgcac atgaaacagg tttagcggtt
     5521 ggcgaatttg ttcacacatt aggagatgct catctttata tcaatcatat cgaacaaatg
     5581 aaagaacaat tgagccgaga aatacgcgca tttccgactt tagttttgaa tcaggaaaag
     5641 cagtcagtat ttgattttga gatggaagat atccaattag aaggatatga cccacatccg
     5701 gcgatcaaag ctcctatcgc tgtttagaat cagaaaggat gagaaaatgt tagctgcaat
     5761 ttgggcacag gatgaaaacg gattgattgg aaaagaagac cgtcttcctt ggcgtttgcc
     5821 aaatgatctg aaattcttca aacaaatgac agaagcaaat acactagtga tgggacgaaa
     5881 aacattcgaa gggatgggta gccgtccgtt gccgaatcgt cagacgattg ttttgacgag
     5941 agattcttcc tataaagcag aaggcgtgca tgtcatgcat agtgtggaag acgtgcttgc
     6001 tctggaaaaa gaaacagacg gtatcttttt catcgctgga gggtcagctg tttatcaaga
     6061 atttctgcca ttttgtacga tattataccg tacagtcatc catcatgtat tcgatggaga
     6121 tgcttatttt ccacctgttg actggtctga ttggtcactg atcaatacta gtcaaggaga
     6181 aatggatgaa aaaaatcgtt atccgcatca atttgaaacg tatcagcgta atgagaatcc
     6241 tgtaaaaagc gggacaggcg aattgacgat ataaaaaagc tatggataga ctagctgtta
     6301 tttagagctg tcccatccat agctttattt tttttattaa gtaaagaata aaacagagaa
     6361 gtacataaaa cctgcaccta acataacaaa caaatgccaa acaacatgca tatagcggat
     6421 agaattcaag ctataaaaga atgcgcctat cgtgtaagaa agcccgcctg ctaccatcaa
     6481 aataacacca gtcattccaa gagagtgata aagaggaaca gcagccacga cacagagcca
     6541 ccccataaaa atatagatga tcgtagatac ttttgatact ctctcccgtt tatgcaacgt
     6601 caacgcttta tatataatgc caagaatcgc aaacaaccag attaatacaa ataaagtcca
     6661 acctagccat cctcggatac tcagcaaaca aaaaggagta taacttcctg caatcaaaag
     6721 aaaaatcgaa ctatgatcga atacttggaa cacctttttt gcttttgtaa aaatcaatga
     6781 atgaaacaaa gtagacgtta aaaataaaag aatcatcatc gatccataga tagcataaga
     6841 gacaatatgg agggaggaat gcaagtgggc accttttact aaaagaacca ctagtcccgc
     6901 gatactcaat ccagtcccaa ttccatgggt gactgcatta aacacttcat ttaatatttc
     6961 atacgttcga ttaaatttcg ttttttccat gaaaacggct cctttactag gtgtaattaa
     7021 tgaaaaatca ttatttgtct gttatactaa aaatagaaat tgtcttacat gtagtttaac
     7081 atgaataagg catcttgtta aagaaagaga gagagaaaat aatgacaaat gtaaaaatcg
     7141 taacagattc ttcatgtacg atggaaaaaa atgtacgcga tgctttaggc attcgtgtca
     7201 tgccgctatc catcatgatc gacggaacag tatatcccga tgatgaccaa ctagacggcg
     7261 aaagatttat ggaaatgatg gcgaaagcaa aaaatctgcc caaaacaagc caaccaccaa
     7321 ttggagaatt catctcgatg tatgatgagc taggagaaga cggcagtgaa gtgatttcga
     7381 tccatatgac aaaaggcttg agtggaacag tagaagcagc aagacaagcc agtaatctaa
     7441 caaaaacgaa agtaacggtt atcgacagtg ataccactga ccaaggatta tcttttcagg
     7501 taattcgagc agctgaatta gcaaaagaag gtaaaacagt agagaaaatc cttgaagaag
     7561 tcaaaaaagt acaagacaat acaaaattat acatcggaat atccacacta gataacttgg
     7621 taaaaggtgg aagaatcagc cgaacaactg gtttgctgtc tagtgttttg aacatacgcg
     7681 tggtgatgaa tttcgcccat tctgaattaa tccctgtaac taaggggcga ggaaagaaaa
     7741 cgttcgataa atggtttaat aatttgaaaa atgaactgaa agaattgcca aatgtgcgac
     7801 aaatcggtat ttcacatgcg gatgcaaaag aattggcgca agaattcaaa gagggtttgc
     7861 aagaattatt cccggacatg catatccctg tactgcatac gaatccggtt atcgctacgc
     7921 atactggtaa agacgcgttt gcaatcatgt attatgtaga ttagtcgaaa agaggataag
     7981 ccctcagcct gaaaggcttg cagcttattc tctttttctt tataaaatta ggaaaataat
     8041 tgttttcttt tttcataaag actacctttt tgataaaatt tttgataaca ttagaaccaa
     8101 tcgaatacaa gaggtgaaaa aatgaaatta acgaaacgac atttgatcct cgttttgttc
     8161 ccattattaa ttgcgatcat catctatgca ggtttagctg cttctatacc gaaagctggc
     8221 ccactaatga aaccagtaac tactgtgaga gaagatccaa ataaaaaaga aaaacttcac
     8281 tacatagcta tcggagattc gctgactgaa ggagtaggag atcaaaccaa gcaaggagga
     8341 ttcgttcccc ttgtcgcaaa tgatctaaaa gaccgttata atttgaccgc ggttgaaaca
     8401 gaaaactatg gtgtaaatgg tgaacgaagt gaccagatct taaagcgagt aaaaacaaaa
     8461 gaagaaattc gaaacaatat caaaacagct gattttatca cactgactgt tggcggtaat
     8521 gatttgatga aagttatcca aaataattta ttcgggttaa cggtcaattc ctttaaaaag
     8581 ccactgaaaa actatcagga aaatgtcaca gaactattgg aagaatttcg atccctcaat
     8641 aaaaccgcac ctatttacat tttaggcatc tacaatccat tttatttgaa ctttcctgag
     8701 atcactgaca tgcagacaat tgttaacaat tggaacaaag gaacagaaac ggtgacaaaa
     8761 aaaatgccga actgctactt tatcccaatc aatgatttgc tttatcaagg attgaatcag
     8821 gaagtaggtg tcacagaaga aggaacagat caaacaacta gcacgacaga aaatctaaat
     8881 ggcatcaaaa ataatgtgct ctatgaagaa gatcatttcc atccaaataa tttaggttat
     8941 caattgatgg caaatgcagt gagagatcag ctgatcgaga cacaaaacca atggttagag
     9001 aaggaggatg atcaataaac atgaaaacaa gatctgacgt atcttcaaac aaaacaaaga
     9061 aaacaaatta ctggaaattc gccttcctta tccttcttgg ggtagtactt ggttcgaccg
     9121 tatttttagg cagccgtatt tttgctaatc gggaaccaga actcccagaa ataccagctc
     9181 ttacggaaag acaaggagat cctgtcttaa cgatcaattc aaataaagaa aaagtcaatc
     9241 aaatcatcag ctttttcctt agtgagtatc aaaaagatag tgatattgaa tataaatttt
     9301 atttggaaaa tgaagcacta ctcaatggta ctttcgaagt actaggtttt cctatcgatt
     9361 tctatttata ttttgatccg tatgtgatgg aaaacggaaa tgtccaacta aaagctagaa
     9421 gtctttctat cggaacattg agtttgccaa tcaaagatgt gatgaatatg atcaagagaa
     9481 actataaatt accagattgg atcgaagtca atacagatga tctgactatc atgcttaggt
     9541 tggatcaatt ccgaatgcaa aacgggatgt atattaaagc agataaaatc gacttagtaa
     9601 atgatgacat tcggtttagt ctatatttgc caaaagatac ctctgatgct tcaaacaact
     9661 caaataccaa caaaactagc accagcagta ctgaaagcaa gacaaaagaa aataaaaaat
     9721 catcggatga gtagtattag agggggaatc ataatggaac gagcaatatt tgcaggaggc
     9781 tgcttctggt gtatgatcca gcctttcgac acgcttcctg gtatccatac agtgatgtca
     9841 ggatatactg gagggcatgt accagatccg acttatgaac aagtaaaaag ccaaacgact
     9901 ggacatacag aagcagtgga gattatctat gatccaacat tgatttcata tgaacaatta
     9961 gtagaacttt actggcagca aactgatcca actgacgctt tcggacaatt cgaggatcgc
    10021 ggtgacaatt atcgcccggt gatcttttat ttgacagaag aacagcggat cattgctgaa
    10081 aaaagtaaga aacagttagc tgaaagtgga cgtttcgaac agccaatcgt tacaaaaatc
    10141 gaaccagccc aaccatttta tctagcagaa gaagaccatc aagactatta taaaaagcat
    10201 cctgaaaatt ttaaaagaaa ccatgcacgc cgggctgcct ttatcgaaga aaattggagg
    10261 aacgaagggt gaatcgaagc ttttttcatt atttaatgac actgcgaggc ggtggcacac
    10321 atgatgcttt atctgttttc gctacagaag ccggtaaaga catccaattt cctaagcaca
    10381 gctctagcta cgaagaaatt tctaattatc tggaactgaa tgtcgactat ttgccgtcca
    10441 tggatatctt tgacgaagca tgggaaaaat atttagaaaa taatcatgac atataagaga
    10501 tagttttgta ctttatgatc aaaaaagttt tttgtccaat agcctaattg aaagaattat
    10561 ttttatttca gctatctagt cgcatttttt tgatattttt cttataataa agaaagaatg
    10621 aaaaaaagag ggatggattg atagagattg acaaaaaaac aacgctatat taatcgcttc
    10681 tggcttgtat tcaaatactt acttgtttct gtattccttg cgttcatgtt gcggggattt
    10741 ttattcatcc cggtaccagt ggaaggcaat tcaatggaaa acgtgttgaa gcaaggtgat
    10801 atggttgtga tggaaaaatt ttcggagatc agacgatttg atattgttgt ttttcaatta
    10861 gcagatggca cgatttatat caaacgagta atcggtcttc caggagaaaa tgtcagctac
    10921 caaaatgatc aattgaagat caatgggaaa gtagttaagg aaccttatct aactaaaaac
    10981 ctgaaatcag atcatgcaaa tgcatcctat acgacggact ttacactaca agagctaacg
    11041 ggacaatcca agcttccgga agataattat tttgtacttg gagataatcg aagagtttca
    11101 aaagatagtc gttcgtttgg cacgatcaat aaaactgata ttctaggtaa agcaagattt
    11161 gtctattatc cacttgatga aataaaatgg atccagtgag aaacaataaa gcgtgcagtc
    11221 tgttgagact gcacgcttta ttgtttaatc caaaatcagt ttactacctt agtacgtaaa
    11281 atcaaacagc tcgtttaaac acttttctag cctctaaagt tttgataaat gaataaacaa
    11341 ccattatttg aatagggatt tggatcatat tcctgatgaa tctacctaga aaaattactt
    11401 tgaaatctgc accaaacaaa acagacatca gccaagaatt gataaaaagg ctgactggga
    11461 tcataatcaa taattgggca gcgatgacgg aaggcaggtt tatcttttta tgatataaaa
    11521 ataagccatt aataaatcca ccaaggaaag ccgtgataag aaaagcagct tgaaacgttc
    11581 ctgaaccaaa tatcgtgaaa tttaaagtgt tcgacaacat ttcaagcaaa ccagcccaga
    11641 tcggtccata tagataaccc ataagcgcca tacttaaaaa tccgaatgtg atttgaaaat
    11701 aagcaggacc aatcgctatc ttgtataaga tgacatcaag ggctaataaa agagcagagg
    11761 tcgacaacaa tcttgcattc atttcgggca atctaaaaac gtcttttgca atcaaagtgt
    11821 tcaaaatatt ctctcctttg tcagttaata ataaaaggat accataaaat caccaatgtg
    11881 tcatgacaca ttggtgattt acatacactg gcatctaaca attaaaaaaa gggtgagaag
    11941 actgctttta taaactgtaa aaaagagatt gtacgaataa aaaaaataga ggcggagaat
    12001 acttatcgta tcatccgcct ttgttttttt gttataaaaa acgtcagatt agttatctgc
    12061 ttacatcttc atccatacgg gttctaatgt atttccgtct agatcttgga cttctaatga
    12121 aaacatttct tcttcaggca ttcccatatc tacttcgtgg aaagtaccac cattttcttt
    12181 tgcaatttga gcaaattttt ttacttcatc cgcattttca acactaaacg aaatcaaagc
    12241 accacttgtt tttttattat cagctatttc tttatctttt ataaattttt tataaaaatc
    12301 atgagagaga agcattatcc aaaaagtgtc atcccatacc attgaaccag cttcttcagt
    12361 agaaaattct tcattttttt taaaacccag cttttcataa aaagcaacgg aacgattgac
    12421 atcagcaaca ggaaaattaa caaaaaccat actattcatt tgtttcgtct cctttctgtt
    12481 ttgttcgtct atcatctctt atttaggaaa cgctgtcaaa aatttagatt catatataga
    12541 aaccaaaata aaaatttgca tcatcaaaaa agtgaagatg tgatagaatg aggactggtt
    12601 cactttcact taactaatag atggaatttc tttataataa aaaagtgaat ttatattgaa
    12661 aaagaaaaaa acaagtaaag ccggggaata ttccggtatg aaaggaatgg agagaacaat
    12721 gaccatccaa tggtttccgg gacatatggc aaaagcccga cgagaagttt ctgaaaaaat
    12781 gaaatatgtg gacatcgtat ttgaattggt agacgcacga ttacctttat cttctcgtaa
    12841 tccaatgcta gatcagatca ttcagcaaaa acctcgcctt gttttattga ataaagcgga
    12901 tctggcagat ccacaacaga caaagctgtg gcaagaatat tttcgtaaac aaggccatct
    12961 tgcattagct atcacggcac aagaaagtaa aggaattaaa ggtctagtac ctgcggctaa
    13021 agaagcattg aaagaaaaac gggcaagaga tgctgctcgg gggatcaagc ctcgcgctat
    13081 tcgggcaatg gtcttaggta ttccaaatgt tgggaaatct acgttgatga atcgactagt
    13141 aggcaagaaa attgcacaga caggaaataa gcctggcgta acaaaaggtc agcagtggct
    13201 tcgtttaggt aatgagttgg aattgttaga cacaccaggg attttatggc ccaaatttga
    13261 agatgaagaa atcggcaaaa aactagcgct gacaggcgcg atcaaagacc agcttgtcca
    13321 tttagatgat ttagcgattt acggattaga cttttttgcc cgatattatt caggaagaat
    13381 cagcgatcgt tatggtttat caatcgagca agaagaaaaa ttggcaccgg aattactgat
    13441 ggatattaca gcaaaacgtg ggtataaaga agactatgat cgtggcagcg aattagtcgt
    13501 gcatgatatt cgtcaaggaa aacttggacg atacacactg gatcgttgcg aagaaatgga
    13561 gcaaaaaagt gatgactgaa tccatccaaa gtatcaaaga acgattgaaa agcatacaga
    13621 gaatagatga tcctacacta gaatcattaa gaaatgatcc acgcaaaggc gtgcagcaag
    13681 cactggcttc ttttgaacga cgattgaaaa aggaagcatt aattaaggaa aaatatgaag
    13741 aaatgttttg ctttgaaaag caagggtacg cacaaggtca ccaatggatt gccggcattg
    13801 atgaagtagg aagaggacca ttagctggac ctgtcgtggc agctgcagtt atcttgccga
    13861 aaaatgagat catattaggg ctgaatgact ccaaacaatt atcagagaaa aagcgagaac
    13921 ttctttatca acaagttcaa gaaaaagccc tggcaatcgg gatcgggatc gttgatcagc
    13981 agacaatcga tgatgtgaat atttatgaag caagtaaatt agcgatgatc caagcagtag
    14041 aaaaattgtc cattgtacca agctatttgt tgatcgatgc gatgaccttg gatttgccta
    14101 ttcagcagga aaaaataatc aaaggggatg cacgatctgt gtcgattgct gctgcaagta
    14161 tcattgctaa agtatatcga gaccaattga tgaaagaata tgacgtgctt tatcctggat
    14221 atggtttctc gcataatgca ggttatggaa caaaggaaca cttagaagga cttgaacgac
    14281 aaggaattac gccaatccat cgtaaaagtt ttgcacctgt aaaaaaatat ttacaataaa
    14341 aaacagccgc ttttacgtat cttattagaa agagagacga aaggcggttt tatgatgtat
    14401 caaatagaag aaaatttatt gaaacttgct tatacaaaag gaatcagtag tcaaggaaaa
    14461 tgggcacttg ctaactggat gatgcagtat ccttataaag aaatcggatt tgaagacatc
    14521 attaagatag gtaaaataac caaacaccgg gagctgtttc gcaaaagttg gcaggaaatt
    14581 cacgctaact ggggcaaaat ccaatcgaaa cagtcgttta tcacttgctt tgatcccaaa
    14641 tatccccctc agctgcttca cttgacctat ccgccaattg tactgtttta taatggtgat
    14701 ctatctttac tttcttgtaa catgttaagc gttgttggtg gaagacacac ttctgcttta
    14761 gccaaaaaaa cagtttatta tttactaaaa ccagtgatag aagctggata tgtgatcgtc
    14821 agtggctgcg cgaaagggat agatacatac ggtcaccaag cagcaatctc tcatggtgga
    14881 cggacaatcg cagtcattgg aacagggata gagcaagtct atcctgctga aaatcgtgac
    14941 ttgcaagagg tgattgcaaa tgatcatctt cttttgagtg aatatcctcc tcaatccggc
    15001 ccgaaaaagc attgttttcc aatgagaaat cggattatcg cagctttgtc taaaggaaca
    15061 tgtgtctttg aagccaagaa aaaaagcggc tctctgataa cagcacagca agcattggat
    15121 cttggaagaa cagttttttc tgttccagga aatattttaa cagaacattc taccggatgt
    15181 catcatttga tccaagacgg tgcagtttgc acggtttcgg gtgaagatat cttggcagaa
    15241 tttaaagaat aacaccatac ttttcatttg cttttcttga caaacgtttt ctgaatggat
    15301 atgattagct acgatttgta tgaatcctct tttaatagta ggaacacgac aatggaaagg
    15361 agtccattca gtaatggcgt ataaatattt agtgattgta gaatcacctg caaaagccaa
    15421 gacaattgag aaatatcttg gacgaaacta taaagtaatg gccagtgttg gccatataag
    15481 agatttaccg aaaagtaaaa tgggtatcga ttttgaaaac aattatgaac cccattatat
    15541 ttctatacgc ggaaaaggcg atgtcatcaa aagcctgaaa gccgcagcaa aaaaagctca
    15601 aaaagtttac ttggcaagtg acccggatag agaaggagaa gcgattgctt ggcatttagc
    15661 gtacctactt gggttggatc tgaaagaaaa aaatcgggtg gtcttcaatg aaatcacaaa
    15721 agacgcagtc aaagcagctt ttaaggaacc aagaacgatc gatgtagatt tagtagatgc
    15781 acagcaagct cgtcgtacct tagacagaat cgttggttat tcgatcagtc ctattctctg
    15841 gcgtaaggtc aagaaagggt taagtgcagg acgtgtccaa tctgtcgctt taaaaattat
    15901 tattgaccgt gaaaaagaga tccgagaatt tgttccagaa gaatattgga gcatcgacgg
    15961 taattttaaa aaagctcgca agaaattcaa agcaaatttc tggggaatcg acggtaagaa
    16021 aaagaaatta ccagatgcac aaagtgtaaa agaagtcact gctagaattg atggaacaga
    16081 cttcgaagtt aaaaaagtag aaaagaaaga gcgcaaaaga aaccctgcga atcctttcac
    16141 tacaagtagc ctgcaacaag aagctgcccg taagttaaac tttcgaacaa gaaaaaccat
    16201 gatggtagca cagcaattat atgaaggaat cactttgggt aaacaaggaa cagtcggctt
    16261 gattacatac atgcgtactg attctacccg gattgccgat acagcaaaag cagaggccgc
    16321 agcatttatt gaagatacgt atggaaaaga attttctaga catgatcacc gaaagatcaa
    16381 aaatgctcaa ggcgcacaag atgcccatga agctgtgcgt ccaacgagtg tgcttcgtac
    16441 accagatgaa ctcaaacaat atttagataa agatcaattg aaactttata cattgatctg
    16501 gtcacgtttt gtggctagtc agatgacacc agctatttta gatacaatga aagtgacatt
    16561 agaacaaaat ggtgtcatct tcattgcgaa cggttcaaaa atcaaattca aaggattcat
    16621 gcaagtgtat gtggaaggta gagatgacgg caaagaagag aaagaaaata ttcttccaga
    16681 acttgaagaa ggcgatgtag tccaatctgt tgatattgaa ccaaaacaac attttactca
    16741 accaccagca cgttttagtg aggctacgtt gattcgtgca ctagaagaaa acggggtagg
    16801 gcgaccttcc acttatgcac caacacttga tacgatccaa agacgctact atgtaaaatt
    16861 gacacaaaaa agatttgagc cgactgaact aggagaaatt gtcaattcat taatctgtga
    16921 attcttccct caaatcgtcg atatccattt cacagcagaa atggaaggag atttggataa
    16981 aattgaagaa ggcacggaag cgtgggtcaa agtggttgat cgtttctata aaccgtttga
    17041 aaaagaactg acgaatgcgg aagaaaaaat cgaaaaaatt caaatcaaag atgaaccagc
    17101 aggattcgat tgtgatgtct gtggacatcc gatggtgata aaattaggga aatatgggaa
    17161 gttttatgct tgcagtaatt ttcctgattg tcggaatacg aaaccaatcg taaaagaaat
    17221 cggtgtgact tgcccagtat gtcatgaagg acaagtcatt gaacgaaaat ctaagaaaaa
    17281 cagactattc tatggttgtt cccgtttccc agaatgtgaa ttcacttctt gggataaacc
    17341 agtgggcaga aattgtccaa aatgtgacgg atatctggtc gagaagaaag tcaaaggcgg
    17401 taaacaagta gtctgtgtaa atggagatta cgaagaagac atccaaaaat aatagaaaag
    17461 aggttctttc attgaaaatg gtaaatgttg ttggagcagg gcttgccgga agtgaagctg
    17521 cttggcagat cgcacaagca ggagtccctg tccgtttata tgaaatgcgt ccagtcaaat
    17581 cgactgaggc gcatcacaca agtaattttg ctgaactagt atgttccaat tcattaagag
    17641 gaaacagttt ggctaatgca gtagggttat tgaaagaaga aatgcgccga ttgaattcgg
    17701 tagtaatcac ttcggcagat gaaacagcag tgccagctgg tggtgcctta gctgttgacc
    17761 gcgattcttt ttctgaaaca attacccgaa aagtcaaaga gcatccatta gtaacagtga
    17821 taaacgaaga aattactgaa ataccggaag gaatcaccgt agtagcaaca ggacctttga
    17881 cttctcatcc attagcagaa agcatcaaag cattcaatgg ttcagatggt ttttatttct
    17941 atgatgcagc tgcaccaatc gtagacaaag ctacgatcga tatggataaa gtatatttga
    18001 agtcccgcta tgacaaagga gaagcagctt acttgaactg tccgatgaca gaagaggaat
    18061 tcaaagcttt ccatgaggca ctaatcaatg ccgaagtggc agaacttaaa tcttttgaga
    18121 aagaaaaata ttttgaagga tgtatgccaa ttgaagtaat ggcacagcga ggattcaaaa
    18181 cgatgctttt cggacctatg aaaccagttg gattggaaga tcctaaaact ggcaaacgtc
    18241 cttatgctgt tatccagctg cgtcaagaca atgctgcagc ttctttatac aacatcgtgg
    18301 gctttcagac tcacttgaaa tggggcgagc aaaaacgtgt tttccggatg attcctgggt
    18361 tagaaaatgc tgaattcgtt cgctatggtg tgatgcaccg aaacagcttt atgaattcgc
    18421 cagaattgct gaaaccgact taccaatcaa cgaaacgcga agatctgttc tttgcaggtc
    18481 agatgactgg tgtagaaggc tatgttgaaa gtgcagcgag cggactactt gctggaatca
    18541 acgccgcacg cttagcaaaa gaaatggaac cagtcgagtt tccacaagaa acagctattg
    18601 gaagtatggc ttactacatt actcatgcgg aaggaaaaca tttccaaccg atgaatgcca
    18661 actatggtct gtttcctgaa ctgccagagc gcattcgaga caaaaaagca agatatgaag
    18721 caattgcaca acgagcttta aaagctaatg aggcaataaa aacagaatta gaaaaaactg
    18781 caatcccaaa tgagtaagga aactccatga agagggtgag acggatagtc tcgctctttt
    18841 tcttgcgttt ttcttaagaa tttctcaaac aaaatgtgag gaattgcgcc aattgtgaac
    18901 aaacttagaa atattggtta aattctgaca atttatttgt ctttttataa acgctgtgct
    18961 aaattattct ctgggacaaa aggaaggtga gaaaatgaca gaagccacgt gggaacaaaa
    19021 atttttgcgt tatttgattg tcgaaagagg atattcagaa aaaacgaaag aagcatatga
    19081 agaagacatt ctccatttca aacatttttt ggaggaatct ggagacgctg atctgctgaa
    19141 ggcggaatat tttacggttc gtgtctattt gagtgcatta gctgatcatc gtcctagcta
    19201 tagcagaaat tctatcagtc gcaagatagc cagcctacgt tcgttttatc agtatcttct
    19261 caaagaagaa gtgataaaag aaaatccatt ttcttacgtt catctcaaaa agaaaaattt
    19321 gagactgcca cgtttttttt atgaaaatga aatgcaagca ctttttgaca gcgtagcagg
    19381 agatagtcct ttggatttga ggaaccgcgc tttactggaa gtcctttacg ggtcaggcat
    19441 ccgtttaagt gagtgtagca gtttgtcggt atcagacatt gattttgatt cagaggtcat
    19501 gttgatccat ggtaaaggga acaaagagag atatgctcct ctaggttctt ttgctcaaga
    19561 tgctctgcaa gaatatttca gggaaggccg ccaggtattg atgacaaaat atcacgaaga
    19621 gcatgatatc gtttttgtga atcatcatgg aaaaccaatc acgcctactg gaattgaata
    19681 tgtcttgaat caaatgatca aaaaaagcag tttggatagt gatatccatc ctcacatgct
    19741 gcgccataca tttgctactc atttattaaa taatggggca gatttgcgga cagtccaaga
    19801 attgctagga catgccaatt tatcgactac ccagatctat gcccatgtaa caaaagaaag
    19861 tttacaaaaa aactatcgtt ctttccatcc aagagcgtaa aaataaggaa aagaacaaat
    19921 ttaggaggaa acaaacatgg tcgaatcacc atttcattca accactatct gtgccgtcga
    19981 aaaagacggg aaatttgcta tggctggaga tggccaagta acaatgggtg aatctgttgt
    20041 catgaaaggc actgcaaaga aagtacgacg tatctacaat ggagaagttg ttgtaggttt
    20101 cgccggaagt gttgcagatg cgtttacttt agaagaaaaa tttgaaggca aactaaacga
    20161 atataacggg aatctacaac gcgcagctgt cgaattagct caagaatggc ggacacagca
    20221 atctatgcaa aaacttgaag cgatgctgat cgtaatgaat gataaagaaa tgcttcttgt
    20281 ttcaggtaca ggagaagtta tcacaccaga tgatggtatc ttagctatcg gatcaggagg
    20341 gaattttgcg ttggctgcag cccgagcaat gaaaaactat gctcaaaacg acatgagtgc
    20401 gaaagaaatt gcagaaaatg cgttaaatat tgccgcagat atctgtatct ttactaatca
    20461 taatattatc gtagaagaac tgtaagaggt gagtagatat gtatgaatta aaacaaacac
    20521 caagacaaat cgtaagtgaa ttagatgaat atatcgtagg acagcacgca gcaaaaaaat
    20581 ctgttgccgt tgctttgcgt aaccgctatc gccgtttgca gttagatgaa aaaatgcaac
    20641 aagatattac accaaaaaat atgctgatga tcggacctac aggggtcggt aaaacagaaa
    20701 tcgcacgacg tttggcaaaa atcgtcaatg caccatttgt caaagtcgaa gcaacaaaat
    20761 ttactgaagt cggttatgtc ggaagagatg tagaatcaat ggttcgggat ttagtagaaa
    20821 atgcgatcca aatcgtagaa aaagagcagt acagccgtgt atacacaagt gctaaaaaaa
    20881 atgcagaaaa acgtttggta aaaatcttgg tacctggtat caaaaaagaa cagaaacaat
    20941 ccactaatca atttgaacaa atgatgaatc tttttaatgc tagtcagcaa aataccgctg
    21001 aaccgcaaga agaagtgaca gaagatattc gaatcaatcg ccgtacagtc ttggaacaac
    21061 tacaaaaggg tttactggat agtcgtgaag taaccattga agttgaagac caaaagaaac
    21121 ctgcaccaat gatgaacaat ggattggagc aaatggggat cgacctaaat gaaactctag
    21181 gtgctttgac accgaagaaa aaagtggaac ggacggtaac agtcaaagaa gctttagagt
    21241 tattgatcaa ggaagaatca gcaaaattag tcaaggatgc tgatatccat agtgaggcaa
    21301 tcaaattagc ggaagaaagt ggaattattt ttatcgatga aatcgataag atcacttcta
    21361 aatcacagca atctggagaa gtatccagag aaggcgtcca aagagacata ttgccaattg
    21421 tcgaaggttc acaagtaaac acaaaatacg ggatcatcca aacagatcac gtcctattca
    21481 ttgcttcagg agctttccat ttgtctaaac caagtgatct aattccagaa cttcaaggac
    21541 gcttcccgat acgggtagaa ttagatgatt tgacagctga agattttgtg aagattctga
    21601 ccgaaccaaa taatgcttta gtaaaacaat acgtcgcgtt gatcggtaca gaaaatgtgt
    21661 cagttacgtt tacaaaagaa gcaattgaac ggatcgctca aatcgctttc gatgtcaacc
    21721 gtgacacaga taatatcggc gcacgcagac tgcataccat tttggaaaaa ctactagaag
    21781 atctattatt tgaagcacca gatatgcaaa tgggagaaat aacgatcaca gaagcatatg
    21841 tggatgacaa acttggctca attgcggcaa atgaagattt aagtcgctat attttataaa
    21901 aaatcggagg acgacaatga cagacttgct aacgaaaacc cgcaagataa acacactttt
    21961 gcagcaaaag aatacttttg acatgcaagc agatttgcct tacgataaaa tggctgtcac
    22021 actaggtggt attcttgaca gcaacaccta tatcatagga agtgacggag tattactagg
    22081 ttataatgaa cgtcacgatg tgaacaatga acgaatcaaa acaatgtttg ttgagaaaca
    22141 atttcctgaa tcttatactg atacagtggc acagttgatg aaaactgaag ctaatatccc
    22201 aattactagt gatattactg catttccggt agagtcaaga gagaaatatc catttggtct
    22261 gacgactgtc gttccgatct ttggagctgg cgaacgtttg ggtacgatta tcttatctcg
    22321 tatggaacaa tcgtttgcag atgacgatct agtcttagca gaatatggag ctacggttgt
    22381 cggtatgcag atcctttatc agaagtctcg aagcatcgaa gcagatgtac ggagtgcaac
    22441 ggctgtccaa atggcaatca acacattatc ttatagcgaa ttgaaggctg tacaagctat
    22501 ctttgaagca ttggatggag atgaaggtcg tctgactgct tctaatattg ccgatagtat
    22561 cggcatcact cgttctgtga tcgtcaatgc tttacgaaaa ttggaatcag ctggaatcat
    22621 tgaatctcgt tcgttaggca tgaagggaac ttatttaaaa gtattgaatt cccgttttaa
    22681 agacgagtta gcaaaacaca gttattagaa gtaaggggac tggggcagat agaaatgttt
    22741 gttccagcct tcccatttta aaaagtaagt cgaagttgga ggaatggcaa tgactgttat
    22801 tattcaaaat gaccagctga tcgccgaaat cgctgaacac ggtgcggaat tgatcagtct
    22861 gaaatcaaaa gaacatggtt tagagtatat ctggcaaggt gatccagcgt attggggcag
    22921 acatgcaccg gttttattcc cttttgttgg acgtttaaaa gatgatcagt atgtttatca
    22981 agggaaaaca tatcaaatgg ggcaacacgg atttgctcgg gatatggatt ttgaagtcat
    23041 tgaacatgga caagaaatgg ctagtttcat cttaaggagt acgccggaga cattagaaaa
    23101 gtatccattt gattttgaat tagtgatcag ctatgaatta ggaggagacg gcatcacggt
    23161 tcattatcaa gtggcaaata ccggtgaaaa agaaatgttc ttttctatcg gtggacatcc
    23221 tgctttcaat gtacctcttg aagaaggatt gtcatttgaa gatttctatc tggcattttc
    23281 tccattgaaa tcaagattac gcctaccatt gaaaggccca ttcatcgata tggatcaaaa
    23341 gacgttaggt cagacaaata cgaatctggc tttgaatcgt gaactttttg agcaagatgc
    23401 cttggttttt gaaacaaggg gtctaaatgc gtacgccatt cgaagtgaaa aatcttccca
    23461 tagcgtaact ttaagctata aagatatgcc ttatgtaggg atctggtctc cttatccaaa
    23521 agaagcgcct ttcgtttgta tcgaaccgtg gtgcggaatc gctgacacgg tgaacagtag
    23581 tggaaacttg atggaaaaag tagggatcaa tcaactaggt gcccatgaaa tcttcaagac
    23641 aaaatattcc atcacagtga agtaagaggt tgtgaccgag aagttagcag ggaacaccgt
    23701 ttaccaaggt gtgtaaaaaa gctatcctgt aagtaataag ggagtgtgac atcgatttgt
    23761 cacactcccg ttttatctct ttggaatgaa aaagtatcag gattttttct tgctgctatt
    23821 caaaccaaac gggattcgac tttcagtatg gtttctgata cgcttgatat tgtctttatg
    23881 ccgataaaag ataaaggaag aaactaaaaa ggcaatgatc gtcagtaacc aattgggctt
    23941 agccaaaatc acaggagcta cataaggaag gacgatcgta gataaagtga tcaatactgc
    24001 actgatcata ctgctcaaac tcaccatact tgtacaaaat aaactaacga taaagatgat
    24061 acaagaataa acaaaaaata caggattaaa tcctaaaagc attcctgcac ttgtcgcaac
    24121 agcttttcct cctttgaacc cagcaaaaat cggaaatgta tggccaagaa tggcgcatac
    24181 cccaaaccaa agagggttaa tcccagaaag gtggaagatc atcggcaaac aagtagccaa
    24241 tgtacctttt aaaatatcca taagcaggac aacaatcccg gctgttttgc ctaatacacg
    24301 aaatgtattg gtcgttccag tattaccgct cccgtgttgt cggatatctt ttttgaaaaa
    24361 caatttgcca atccagacac cggaaggaat agaacctaaa agatatgcta aaataagtaa
    24421 taagatcaat ttcatttttt accctctttc aagttgaaaa ctatccttta ttttagcatg
    24481 aataggtaac aggaacaatt gaaaaaatga aagatacaag tataatagat ggaataaaaa
    24541 gaaaagcaag gggtggcaga aatgtctgta aaaaatccaa cacaagaaac gattttcaac
    24601 tatttggaag aagcaaaaaa tattgccgtc gtaggcttga gcaataaacc agaacgaaca
    24661 agttacatgg ttgcacaggt attgcaagac aatggatata aaatcatacc agtaaatcca
    24721 ttattggctg gtgaagaaat tctaggcgag aaagtctatg cgaatttgac agaaatcgat
    24781 gatccaatcg atatcgtaga tgtatttaga cggagtgaat ttttacctga aatagcaggt
    24841 gattttttga aaactgatgc taaagtgttc tgggcacaat tagggattga aaacaaagaa
    24901 gcagctgaga tgctaagaaa agcaggacgt acagatatcg taatggatcg ttgtattaag
    24961 attgaacttg gtaagttgaa agaaaaccaa aaataaaaat gaccacaaag aagagatgat
    25021 cgatagaggg gaatgtcgaa aaagcggtta ggagataatt aaaattgatt tctcgtctac
    25081 cctctttttt ttcacgacct tttctagtat gataatcgtg gtgcggtttt gccgcaaagt
    25141 attattgaag gagcgtttgc cttggcaaaa aaagtaaaca acgaatacaa tgactcgtcc
    25201 atccaagtat tggaaggatt agaggcagtt agaaaaagac cagggatgta cattggatcc
    25261 actgacagcc gaggcttgca tcatttagtc tatgaaatcg tcgataatgc agttgacgaa
    25321 gcattatcag gttatggcag tgagattgat gtgaccatcc atgaagacaa tagtataact
    25381 gttgcagata gcggacgtgg gatgcctgta ggaatgcatg cttccggaat accaacagtt
    25441 gaagtgattt ttactgtgct gcatgctggc ggaaagtttg gtcaaggagg gtacaaaact
    25501 tctggtggac tgcacggtgt aggagccagt gtggtaaatg ccttatcgaa atggctaatt
    25561 gtaacgatcg ttagagatgg cgtagagtat cagcagaaat ttaaaaacgg cggtaaacct
    25621 gacggtactt tgaaaaagat aggaaaaaca aaaaaagcaa acgggacgac tgtccatttt
    25681 ttaccagatg atacaatatt cagtacaacg aaattttcat atgaaatttt agccgaacgg
    25741 ttaagagaat ctgcctttct tctaaaaggc gttaaaatct ctctttctga tcttcgtgga
    25801 gaagaaccag tcaaggaaat ttttcattac gaagaaggaa tcaaagaatt tgttgattat
    25861 ctgaatgaag aaaaagatac attgacacca gttgtttatt tttcaggaga aaaagaaggt
    25921 atcgaagtag aggtcgctta tcaatacaat gacggttatt cagaaaatgt tttatctttt
    25981 gtcaataacg ttcggacaaa agatggggga acccatgaag ctggtatgaa agcggcgatg
    26041 acgaaatctt ataatgaata tgcgagaaaa gttggtttgc tgaaagaacg ggacaagaat
    26101 ctggaaggta gtgatttcag agaagggtta gctgctgtac tttctatccg tgtacctgaa
    26161 aatcttctgc aatttgaagg gcaaacaaaa gaaaaacttg gtacgccagt tgctcgtacc
    26221 gtagtagata acgtcatcag tgaacaaatg ggcttttatt tgcaggaaaa tagcgaaatg
    26281 agccagatgt tggtccgtaa agcaatcaaa gccagagaag cgagagaagc agcacgtaaa
    26341 gccagagaag aaagccgaaa tgggaagaag cgtaaaaaag gcgaatctct tctttcagga
    26401 aaattgacac ccgcacaatc ccgaaaccct aagaaaaatg aattgtattt agtcgaaggg
    26461 gattctgctg gcggatctgc taaacaagga agagacagaa agttccaagc aattctacct
    26521 ttacgaggaa aagtcatcaa taccgaaaaa gctaaaatgc aagatatctt gaaaaatgaa
    26581 gaaatcaata cgatgatcta tacgattggt gcaggagtcg gtcctgagtt ttctatcgaa
    26641 gactgtaatt acgacaaagt aatcatcatg accgatgcgg atacagacgg cgcccatatt
    26701 caagtattac tgcttacttt cttttatcgt tatatgaaac cgctgattga agctgggaaa
    26761 gtatatatcg cgttaccgcc tttatataaa gtatccaaag gacaaggaaa gaagcaagtc
    26821 attgaatatg cgtggacaga tgacgaattg gcagcaatga ttaaaaaagt cggcaaagga
    26881 tatatgctgc agcgatataa aggacttgga gagatgaatg cggaacaatt gtgggaaaca
    26941 acgatggatc cgacttcccg tacgttgatc cgcgtccgta tcgatgatgc tgcgcaagct
    27001 gaacgtcgag taaccacact gatgggtgac aaagtagaac ctcgaagaaa atggatcgaa
    27061 aatcacgtgc aatttacgct cgaagaagat ggcagtatct tagataaaaa agaggatact
    27121 gaaatctctc cttcagtttc taatgattta ttagatgaag aacgagcaga taaaaatgaa
    27181 aacaatcagt tatttgaagt agaataggag gtcacctgat ggaaaatcga caagaagtac
    27241 aagaattaac tttagaagaa gtgatgggtg accgcttcgg acgttattcc aaatatatta
    27301 tccaagaacg tgcactgcct gatatccgtg atggattgaa gccagtgcaa cgccggatat
    27361 tatttgccat gaacaaagat ggcaatacgt atgacaaagg attccgtaaa tcagcaaaat
    27421 ctgtcggaaa tatcatgggg aattatcacc ctcatggtga ccgcagtatt tatgaagcga
    27481 tggtccgcat gagccaagac tggaagttgc gggaagtact gatcgaaatg cacgggaata
    27541 acgggagtat ggacggtgat ccacctgctg cgatgcgtta tactgaagcc agattatcca
    27601 agttaagtgg agagatgcta gcagatatcg agaaagaaac agtcgatctc gtgtggaatt
    27661 ttgatgatac agaaaaagaa ccaacagtcc ttccagctcg atacccaaac cttctcgtca
    27721 atggatcgac aggtatatct gctggatatg caacagaaat cccgactcat aacttagctg
    27781 aagtgatcga tggcacaatc tatatgatcg atcatccaca agcgagcctt gaaaaattga
    27841 tggaatatat tcctggacct gactttccaa caggagggat cttgcaagga aaagatgaaa
    27901 tcaaaaaagc ttatgaaaca ggacgaggaa aagttattct tcgttcgaaa acaaagatag
    27961 aggcaatgaa gggtaataaa cagcaaatcg tgatcacaga aatcccatat gaagtaaata
    28021 aagcgacctt ggtgaaaaaa atggatgaga tccgtttgaa taagaaaatc gatggaatag
    28081 ctgaagtacg agatgaaagt gatagaacag gtctgcaaat tgtcgtcgaa ttgaaaaaag
    28141 acgccaatgc tcaaggaatt ttgaattatt tgttcaaaaa cactgagcta cagatcaatt
    28201 acaatttcaa tatggtcgct atcgaccata tgactccgca tcaagttgga ctgaaagaca
    28261 ttttaagaag ttatatcgag catcgaaagc aagtcataac aaaacgtagt caattcgatt
    28321 tagccaaagc tcaaaaaaga cagcatatcg tagaaggatt gatgaaagct ttatcgattc
    28381 tggatgaagt aatcgcaacg attcgtgaaa gtaaagacaa aaaagacgcc aaaaagaatt
    28441 tagtcgacgt ctttcaattt acagaagaac aagcagaagc aattgtcaca ttgcaattat
    28501 accgtttgac gaacacggat atcacagagc ttcaaaagga atccgaaagc ttgattgcgc
    28561 aaatcacaga gttgaataaa atcctttcaa atgacaaaga gcttttctct gttatgaaaa
    28621 aagaattgcg agaagtgaaa aaaaattatt cttccgctcg tctaacgaca atcgaagacg
    28681 aaatcgaaga aatcaaaatt gatacgcaag tcttagtggc acaagaagac gtaattgtca
    28741 gtgtgacgcg agaaggctat gtgaagcgaa caagccttcg ttcttatagt gcttccaaac
    28801 cagaagaaat cgggatgcga gaaggcgact acttgctata ctcaggtgaa ttgagtacat
    28861 tggaccatgt tcttctgatc acaaataaag caaatgtgat ctaccgccct gtccatgaac
    28921 tacccgattt gaaatggaaa gatgctggtg agcacatttc tcaaacaatc tccaatctgt
    28981 ctgtagacga atcgatttta gctgttttcc catatcaaaa aattcaagca gagaaaacat
    29041 ttgtattcat cagcaagaat ggattgatca agcaaacacg aatgacagag ttcgaaccat
    29101 ggcgcaccta taagagccgt ccgctcagct gtatgaagct aaaggcggaa gaagatgaac
    29161 tgattgctgt gtatctgaaa gaagatcagc caggcttaga tgttttctta gtaacgcatc
    29221 aaggaatggg tttgcgctat cctttaacag atgttcccgt tgttggagca aaggcagctg
    29281 gagtgaagtc tatgaatttg aaagatggag ataggattat caacgggctg cttgtgttag
    29341 cagaaggtga tacgccgatc gtaatcgtta ctcagcgagg agctgttaaa cgcatgcttg
    29401 ctcaagaaat cagccaaact tcacgtgcga agcggggagt gacggtcatg cgtgagctga
    29461 aaaaaaatcc gcaccgtatt atctatatgt cggaaggaag atcaaaagaa ataaccttga
    29521 tgaatcaaaa agggcagcag ttaacgattg acccaactga tttccctata ggtgatcgaa
    29581 catcgaatgg atcatttgcg atggatgaga aaaaaggtgg agaagtcatc gaagttatcg
    29641 atgctcctga gattttcatc gagtcgtaac tgttgtagta ttttaatgaa gagacgccat
    29701 ctgtattaat acaggtggcg tctctttttc attcattttt aataaatcct tatacgtttg
    29761 caaaaaaaga tgaaagtgtt cacaatgctt aaatgttggt atgtcaacgt tgatttaaat
    29821 atatgtaaat aaaacataaa aattgttaaa taaaaaacaa gaaaaccctt gcaaagatag
    29881 ggtgctgtgt tatactaatc acgtaaaaac tgttacataa aacagaaatt taggaggtcc
    29941 atacaatgga gcaatggaat ggatttaaag gtaccaaatg gaaagaaggc gtagacacta
    30001 gagactttat tcaaaataac tacactgaat atcgcggtga tgacagcttt ttggaaggcc
    30061 caacagaagc aacagaaaaa ttatgggatg aattgcaaaa gttaaatgat atccaattcg
    30121 agcgtaacgg tgtctatgat atggacacag aaatcgtatc aactattact tcacacgatg
    30181 caggctattt ggataaagat ttggaaaaaa tcgtcggtct acagacagac aaacctttga
    30241 aacaagcatt catgccgttc ggcggaatca atatggctaa caatgcgcta aaaagcaatg
    30301 gctatacacc aaacgatgaa ttgacacata ttttcactga ctggcgcaaa acacataatc
    30361 aaggtgtatt tgacgcttat actgctgaaa tgcgtgctgc tcgtaaaaac aaaatcatta
    30421 cagggcttcc agacgcttac ggtcgcggac gtatcatcgg tgactatcgt cgtgtggcat
    30481 tgtatggaat cgactatttg atggaacaaa agaaaaaaga tcatgatatg actggttatc
    30541 atacaatgag tgaagaagtg attcgtttac gtgaagaaat cactgaacaa tatcgtgcat
    30601 tagcgcaaat gaaagaaatg gctgcgaaat atggctatga tatttctaaa ccagctgcaa
    30661 atgcaaaaga agctgtccaa tggctatact tcggatattt agcagcaatc aaatcacaaa
    30721 atggtgctgc gatgtcaatc ggacgtatct cagcattcct tgatatctat atccaacgtg
    30781 atttggataa tggagtaatc acagaaaaag aagcacaaga attgatcgac cacttagtaa
    30841 tgaaattacg tatggtcaaa ttcgcgcgta cacctgaata taaccaatta ttctcaggat
    30901 tccctatctg ggctacttta tccattgcag gtatgggatt agatggtcgt tcattagtta
    30961 caaagaatga tttccgtctg cttcatacat taacaaatat gggaccttca ccagaaccaa
    31021 acttgactgt tttgtattct gaacaccttc cagaaggatt cagaacatat gctagcagaa
    31081 tcgctatcga aagttcttcg attcaattcg aaaatgataa tcagcttcgt gaacaatggg
    31141 gttctgatga ttgtgcgatt gcttgctgtg tatcaggaac agttgtcgga aaagacatgc
    31201 aattctttgg cgctcgtgct aacttggcta aaacactatt atatgccatc aatggcggag
    31261 tcgatgaagt gactggcgcc caagttggac cagaatatcg tccaatcaca tcagaaatac
    31321 tagactttga agaagtgaaa cataacttca tgaatatgat ggattggtta gcagaactat
    31381 atgtcaatac gttgaacatc attcattata tgcatgataa atattcttat gaagcagctc
    31441 agctggcttt gatggattct gacttgcaac gcacatttgc tacaggtatt gctgggatct
    31501 ctcatgcggc agacagcttg tcagcgatca aatatgcaaa agtaaaagca atccgtaatg
    31561 aaaaaggaat agcagttgat tttgaagtgg atggcgactt ccctaaatac ggaaatgatg
    31621 atgaccgtgc agatgaatta gtttcatgga tcttggaata cttcatggga caaatcaaac
    31681 gtcataaaac ttaccgcaac tcaaaaccaa caacttcatt attgacgatt acttcaaatg
    31741 ttgtttacgg taaaaataca ggaaatactc ctgatggccg tgaagctgga aaaccacttg
    31801 cgccaggagc aaatccaagt tacaatgcgg aacaaagtgg gttattagct agcctgaact
    31861 caacagctaa aatgaattac ggctgtgcat tggatggaat ttcaaatacc caaacaatca
    31921 atccttcagg tcttggaaaa gaacaagata caagaatcga aaacttgcgt aatgtcttag
    31981 atggatactt ctcaaaagga gcttatcatt tgaatgtcaa tgtattcagt aatgatttat
    32041 tgcgtgatgc aatggaaaat ccaatgaaat atccgaactt gacgatccgg gtatctggtt
    32101 atgcagttaa attccgtgac ttgacaagag aacaacaatt agatgtcttg atgagaactt
    32161 cacacgacag aatgtaaaaa gttcgtgaaa aaagtttttc gacttagcgt cgaaaggacg
    32221 ttgaatagcc atcgcatcaa aatcgaataa tcagtatgct tatttgaaag aaacgattta
    32281 caggggcagg acttgtatgt tccgtccctg taaattttac acataacgaa gcggcaattg
    32341 cgaaaaattt tgttcagaaa gcttcgcttc gacagctaac ttgtacttaa aatgaaatca
    32401 aagaaaagat gtgaacttaa atggaagaaa aaacaatcgg ctatgttcat tctatcgaaa
    32461 ctttcggttc agtagacgga cctggactgc gctttgtcgt gtttatgcaa ggatgccgca
    32521 tgcgttgcca attttgtcac aatccagata cttggaatat tggcggaggg aaagaataca
    32581 cagcagatga acttttagat aaagcagaac gcttcagacc ttactgggga gataaaggtg
    32641 gtattactgt tagtggtgga gaacctctat tgcagatcga ttttttgatc gaactattta
    32701 aaaaagcaaa agagcggaag atgcatacta cattggatac atgcggaaaa ccttttacgt
    32761 atgaagaacc tttcttttca cgttttcaag aattgatgaa gtatacagat ttactgttat
    32821 ttgacatcaa acacatcgat aatgaagagc ataaaaaact aacgcattgg gacaatgaaa
    32881 atattttaga aatggctcaa tatttgtcaa aaatcaacaa accggtttgg attcgccatg
    32941 tactagttcc tgagcgaagt gattatgatg aatatttgat ccgactagac aactttattc
    33001 aaaccttgtc aaatgtagac agagtagaga ttcttcctta tcatacgatg ggaaaataca
    33061 agtgggaaac acttggtttg aaatatccat tagaagggat cgaaccacct acaaaagaac
    33121 gtgtcgaaaa tgctaaacga ttgcttcata cagaagagta tacaaaatat ttaacgcgat
    33181 aggtcgcatc ggaaagaaga taagtattgc ttctttccgt ttttttgcta ttattaaata
    33241 ggagaaatca caaaggagcg aatgacatgt caaaaatttt agtttttggt caccaaaatc
    33301 cagatacaga tgcaatcggg gcagccattg cgttttcaca cttgcaaaat gaattaggta
    33361 aagaaacaga agcagttgct ctaggtacac ctaatgaaga aacacaatac gcattagacc
    33421 actttcagct atctgcacca cgagtagtag agtcagctaa agcagaaaat gcagatgtca
    33481 tgcttgtgga tcataatgaa ttccaacaaa gtatttcaga tatcagcgaa gtaaatattt
    33541 tagctgtagt agaccaccat cgtattgcaa actttgaaac agctaatcca ttgtactatc
    33601 gtgcagaacc agttggatgt acaagtacga ttattttaaa actatataaa gaaaaccaag
    33661 taacaattcc taaagaaatc gcaggaatca tgctctcagc tatcatttca gatacattgt
    33721 tgttcaaatc accaacatgt acgcctgaag atgaagcagc tgcaaaagaa cttgcagcaa
    33781 tcgctgaagt cgatctaaac acttatggac ttgaaatgtt gaaagcagga acaaatctaa
    33841 gtgataaatc agctgcggtt cttcttgatt tggatgcaaa aagtttccca atgggcagtt
    33901 ccaacgttcg tatcgctcaa gtcaatacag tggaccttaa tgaagtgatg gatcgacaag
    33961 cagagctaga agcagcaatg gaagatgaaa tgaaagcaaa tggctacgat ctgttcgtat
    34021 tagttgtcac aaacatctta gacagtgatt cagaattact tgttgctggt aaaggaaaag
    34081 aaaaagtaga agaagctttc caaacaactt tgaccaacaa ccgtgctttg cttaaaggtg
    34141 tcgtttcacg taaaaaacaa gtcgtacctc aattgacggc tgtatttagc taatcaaatg
    34201 gtagtatgaa gcattcaaaa tgaatggata agcgattgat ataaaaagaa acatagaaca
    34261 tactaaaggc cgaagaaatg gaggagaata acagtcaatt atgactcttg ttcttcctcc
    34321 tttttacgta aaagcctttt aagtaaaata aaacggatag aaaagagaac atatatgaac
    34381 tattattcaa aaaataagga aaacttttat caaaaactga ccgatgatcc gcttttttct
    34441 tcattgaccg attatctata tgaacatcga gaacaagaaa cgattctaag agaattgaaa
    34501 aaagaatttt cgcaaaataa attttcccat tttcttgatt tattgatcga tgcaggatta
    34561 atcaagaggg aagaacgaag atatcatttg aactttccaa tatttgatcc taaagattac
    34621 ttgcaacaag ccacttcggc tgctgaaacc atagctgaac aattgaagag actctctgtt
    34681 gatgaacaaa aattagcgat gggagaagtt atttgggcgt attgtttcga agatgaacgg
    34741 aaagaagcgt atttttatgg agtaagaaac agccgagaga cagaattgct gaggacaact
    34801 gcaggaaatg agaagtaccg ttttatcaca ctttcctcta tagaacactt cccgcttact
    34861 ttggctaatt acttttttgt acagaagaat cagcttcctg taacgaaggc attcaaagaa
    34921 ttagccgaac ttattggaga cgtaaatgaa tcgtactttt ttgatcaaat agaagttatc
    34981 gttgacagga tacgtaagaa taaatataaa aatcgccggc ctagtatttt ccatcagtct
    35041 ctacttgtga cgaatactat aaaggaagaa gaatcattca cattagaatt acctattgtc
    35101 gaaaaaaaca attttgagat tgaattacca acattggatc cttcattgac aatggaggaa
    35161 atggcctttc ttaaacgaca aatcttttct gagctgagca agaaatttat accgcatgca
    35221 ttttcttata tcaaagaata tggaacagtt ttagtgagta aaacctaaga gagcagtaag
    35281 caaaaattat actgactctt gcaggaggag cctttctctt cctttatggt taaaagaatt
    35341 atttgagtat tttgcctatc taccacctat tagattcact acatggtctg gaaacaaagg
    35401 aatgcaaaaa aatggtgaga aatgtggaat gacaaaggaa gtagtgataa gaaaagttag
    35461 atttttaaat aatcaatatt atgattcagt caagtatggc attttatggg aagaactagc
    35521 tgattagatt aggaagtcca tttgataatc cagaatcact aagaaacgaa tggcgagtca
    35581 ttaataattt atccaatctt gggttattct tttagattga agtgtttaaa actaaaaaag
    35641 catttatgaa gtcgtaatag tcaggcttca aaatgctttt tattttgttt gttttttctt
    35701 tgccacataa atacacctag actgacgatg aagagcatat ccaacagatt tcctgcgact
    35761 tggatcgtcg aagggttggt cagtggcatt aaagcaagga caaaaaagga aaacaagctg
    35821 taaataaaac aggtgacagg aactaatcga aagaagagcg gacatttcaa ttggctaaaa
    35881 atgagtgaaa aaataaaata agtaaaaatg gataaaatca cgactaaacc attcatgata
    35941 aagaagaaaa gcgtattgtg attaaacgaa taaaaaagaa ttgctaaaaa agaaaacgtt
    36001 ttaaatgcaa tcaatacgct tgaagcttcg tttaacgtac caggatgtcg atgaaaaaaa
    36061 taggcaccga aataaacaga aaacagtaag ccgcaaagag aagacaaaat caaaaaaaac
    36121 ggattttctt cggcttctgt gatatcagaa tgatccaaaa gcaaccagcc taatgcacta
    36181 gaaggcgcat gtgaggtctt aaggtatgaa taacatagaa aaaataagat cgtcaaagtt
    36241 gcaaaaaaag agcctaaatg taaaaaatt
//
LOCUS       DACDIG010000025        33764 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.025, whole genome shotgun sequence.
ACCESSION   DACDIG010000025 DACDIG010000000
VERSION     DACDIG010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33764
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.025"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..810
                     /locus_tag="IV247_001284"
     CDS             <1..810
                     /locus_tag="IV247_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8777363.1"
                     /translation="KEYANIKIIKGYVRSMFDIAEILNYIEFNRTTKIIQSITAPKKN
                     ALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLLTFHDYTLFMITLYLGDRKSETYA
                     LQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTVIQVPEVVMTLLSEWKSVQADQLL
                     KLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRINSIKRRHPDLVHLSPHKLRHTYA
                     TIARQGGADMNQISNALTHSDISTTKIYVNTPDIVDKAVFEAFQRGLNKCD"
     gene            complement(898..1290)
                     /gene="rpsI"
                     /locus_tag="IV247_001285"
     CDS             complement(898..1290)
                     /gene="rpsI"
                     /locus_tag="IV247_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP8777364.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(1306..1749)
                     /gene="rplM"
                     /locus_tag="IV247_001286"
     CDS             complement(1306..1749)
                     /gene="rplM"
                     /locus_tag="IV247_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAP8777365.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(2268..2735)
                     /locus_tag="IV247_001287"
     CDS             complement(2268..2735)
                     /locus_tag="IV247_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAP8777366.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(2855..4393)
                     /locus_tag="IV247_001288"
     CDS             complement(2855..4393)
                     /locus_tag="IV247_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAP8777367.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            4473..5159
                     /locus_tag="IV247_001289"
     CDS             4473..5159
                     /locus_tag="IV247_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAP8777368.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(5360..5887)
                     /gene="msrA"
                     /locus_tag="IV247_001290"
     CDS             complement(5360..5887)
                     /gene="msrA"
                     /locus_tag="IV247_001290"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP8777369.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(5971..6648)
                     /gene="pgmB"
                     /locus_tag="IV247_001291"
     CDS             complement(5971..6648)
                     /gene="pgmB"
                     /locus_tag="IV247_001291"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP8777370.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(6645..7775)
                     /locus_tag="IV247_001292"
     CDS             complement(6645..7775)
                     /locus_tag="IV247_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP8777371.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(7775..9958)
                     /locus_tag="IV247_001293"
     CDS             complement(7775..9958)
                     /locus_tag="IV247_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP8777372.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(9955..10980)
                     /locus_tag="IV247_001294"
     CDS             complement(9955..10980)
                     /locus_tag="IV247_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8777373.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(11025..11852)
                     /locus_tag="IV247_001295"
     CDS             complement(11025..11852)
                     /locus_tag="IV247_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP8777374.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(11849..12916)
                     /locus_tag="IV247_001296"
     CDS             complement(11849..12916)
                     /locus_tag="IV247_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAP8777375.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(12942..13778)
                     /locus_tag="IV247_001297"
     CDS             complement(12942..13778)
                     /locus_tag="IV247_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8777376.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(13775..14644)
                     /locus_tag="IV247_001298"
     CDS             complement(13775..14644)
                     /locus_tag="IV247_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8777377.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(14714..15997)
                     /locus_tag="IV247_001299"
     CDS             complement(14714..15997)
                     /locus_tag="IV247_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP8777378.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(16011..17696)
                     /locus_tag="IV247_001300"
     CDS             complement(16011..17696)
                     /locus_tag="IV247_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAP8777379.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            17971..18996
                     /locus_tag="IV247_001301"
     CDS             17971..18996
                     /locus_tag="IV247_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8777380.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(19591..23244)
                     /gene="rpoC"
                     /locus_tag="IV247_001302"
     CDS             complement(19591..23244)
                     /gene="rpoC"
                     /locus_tag="IV247_001302"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAP8777381.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(23323..26940)
                     /gene="rpoB"
                     /locus_tag="IV247_001303"
     CDS             complement(23323..26940)
                     /gene="rpoB"
                     /locus_tag="IV247_001303"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP8777382.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            27668..28636
                     /locus_tag="IV247_001304"
     CDS             27668..28636
                     /locus_tag="IV247_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8777383.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            28654..29559
                     /locus_tag="IV247_001305"
     CDS             28654..29559
                     /locus_tag="IV247_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8777384.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            29556..30362
                     /locus_tag="IV247_001306"
     CDS             29556..30362
                     /locus_tag="IV247_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8777385.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            30519..31487
                     /locus_tag="IV247_001307"
     CDS             30519..31487
                     /locus_tag="IV247_001307"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAP8777386.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(31804..32328)
                     /locus_tag="IV247_001308"
     CDS             complement(31804..32328)
                     /locus_tag="IV247_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAP8777387.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            32615..>33764
                     /gene="scm"
                     /locus_tag="IV247_001309"
     CDS             32615..>33764
                     /gene="scm"
                     /locus_tag="IV247_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP8777388.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTT"
BASE COUNT         9568 a         7218 c         6335 g        10643 t
ORIGIN      
        1 aaagaatatg caaatataaa aatcataaaa ggatatgttc gttccatgtt cgatattgca
       61 gaaatactaa attatatcga atttaatcga actactaaaa ttattcaaag tatcactgca
      121 cctaaaaaaa atgctttaga agaaaaaaga atacaagaag gtaagcaagc actatcatca
      181 aaagaactta ctaattggat agaagcagta aatgatgatt ttaataatca tttacttact
      241 tttcatgatt acacactttt tatgattacc ctgtatcttg gtgatagaaa aagtgagact
      301 tacgctttgc aatggaaata tattgatttt gaaaaacaaa cggtccgtct taaacatact
      361 ttagataaat atcaaagaaa aaagtttaca aaaggtcgaa aagataccgt cattcaagta
      421 ccagaagttg taatgacatt gctaagtgaa tggaaatcgg tacaagctga tcaattgttg
      481 aaattaaaaa ttaagcaaac gttagatcag tatctcttta cttatactaa accatctgga
      541 gaagtaaatt gtcctgttca tgccgattac ctaaactatc gaatcaattc aattaaaaga
      601 aggcatcctg acttagttca tttatctcca cacaaactcc gtcatactta tgcaactata
      661 gctagacaag gtggagcaga tatgaatcaa atttcaaatg ctttaacaca ctcagatatt
      721 tctacaacaa agatatatgt taacacacca gatatagttg ataaggctgt ttttgaagct
      781 tttcagagag gattgaataa gtgtgattga tatgtgttct taaatgtgtt cttaaaaaca
      841 aaagaagcac tttccaaata attgaaaagt gccagaaacg ttgatatata aaggatatta
      901 acgttttgag aattgtgaag ctttacgtgc tttcttaaga cctggttttt tacgttcgac
      961 catacgtgcg tcacgagtaa gtaagccagc acgtttaaga gctaaacgga agtcaggatc
     1021 aacttctagc aatgcacgag cgataccatg acggatagct cctgattgtc cagcgtatcc
     1081 tccgccgttt acgttaacaa atacgtcata tgcgcctttt gtttcagtaa cagcgaaagg
     1141 ttgattgatt acttcacgca agtcagcatg tggaatgtat tcttcaacat cttttttatt
     1201 tacagtaatt ttgccagttc ctggtactag acgtacgcga gcaactgcgt ttttacgacg
     1261 gcctgtgccg atatattgaa cttgtgccaa tgaaattccc tcctattaga ttaagtttgt
     1321 gatgtctaaa acttctggtt gttgtgctgc atgtggatgt tctgctccac cgtatacgtt
     1381 caattttgta aattgtttgc ggcctaaagt gtttttagga agcatacctt ttacagaagt
     1441 ttcgatcaaa cgacgagaat ttttagcacg caattcgcca gcagtgattg atttcaatcc
     1501 tcctgggtag ttgctgtgac ggtaataaat tttgtcagtc gcttttttac ctgttaattt
     1561 cactttgtct gcattgatga cgatgacgaa atcgccagta tccaaatgag gtgtgaaagt
     1621 tggtttattt ttaccacgta gtacagatgc aacaactgtt gaaagacgtc ccaatggaac
     1681 atcagttgcg tctactacat accatttacg ttctacttcg cctggtttgg ccatatatgt
     1741 tgtacgcacg ttattttcct ccaatttaag ttctgatgtt tgacatacac ttcgagtttc
     1801 cggggctctt tgtggggcaa acaataccat ttaacatatt acccgctaat ctagtcaatg
     1861 tcaacgtttt tctgcttgat tgaaaaattt atatgaaact ttacttcttt tctttctttt
     1921 atataaaagg gtcgtttcct gtcaaaatga aaaacaaaaa gaggctgtga caaaagtctt
     1981 gtcacaaccc cttattttga ataaacggtg ttctcctgcc aactcctcgg tcacaagtca
     2041 caatatccat aaaacataag aagctgtttt ttaaaattgt ttcttgtgac tcggagctga
     2101 acggcaggct tacaacctct tggtaaacgg tgttcaatca gctgaccctc ggtattagct
     2161 caaaaaacgg caaaatatga ggaactattt tgacgtttgt ttctctaata ctcaggtcaa
     2221 aacgctgatt tcacaacctc tttttcggtc aagttcaaca acagttttta cgcgtcgatc
     2281 tctggcgctt tgcccaaatg agcttggatc atccagatac gtttttctgt ggctgttttg
     2341 aattcgataa agatatcttg tgttggatcg tcgccttctt ctccagaaac ttcgattcct
     2401 ttttggtaaa gatctgctag ataacgatag cctgcaacaa gtgtttccaa acgttctgga
     2461 atagtacgat cccagcggcc aatttcatca gggatttttg tgttatctgc aaattcacgc
     2521 aatgtagaat aaggtgaacc gtctaatgtg atcaagcgtt cggatacttc atctaactga
     2581 gcgttcaaat catccatgaa ctcatccatc attgggtgca atgtaaggaa acctggtcct
     2641 ctcatgtacc aatgtgtttg gtgaacaaca acagacattt ggcttagatc tgcaactaat
     2701 tgatttaata cttctttggt ttgttggaat ttcatctttc ttgcctccta acattttctt
     2761 acacttaaaa taatagtaga ctttcaatga aaagtacaac aaaatcattt acctgagaac
     2821 gattttcagt tcgctttttt atccgtaaag ccaatcattt ttcagaatca gctaattctt
     2881 ctcgcagttg agcaaattgc tgataagtct gtgctaaatg cttttgtgtc gaaacaaata
     2941 atggatagat tttctgatat tttttatgat cagtttcttg tactccaact tgctctttca
     3001 attctatcaa ttcctgtgct tcactgatac tttggatctt tccgatgctt ctccagccta
     3061 aaatcacagc tcctaatgcg gatgcttctg tgacagtagg gaaaacaagc gggtaaccca
     3121 gtacatcagc catcatctga cgaaaaatcg gagattgagc aaacccgcct gtagcacgga
     3181 tctctgtgag ctgattttcc tggcaaactc cttttaaaat ccgataaagg ttgagacaga
     3241 tcccttctag aatcgccttc atcatttctt tttctgtatg ttgccgctgt aaaccgataa
     3301 aactgccaaa cgcatctgcg tcccaaagcg gtgctcgctc accaagtaaa tagggatgaa
     3361 aaatcagacc attggcaccc gctggcacaa aagcgatagc cgaaagcagt gcatcatatg
     3421 gattttctcc tttttcttta gcagattgaa cgacttcctg taaaaatgta tgccctgccc
     3481 aatcaaagat gccagcacca ttgctagtcg ctcctcccac tacccaatgt ttctgatcca
     3541 gaacataaca aaacgtctcc atttctggat ggaactgagg ctgagtagta ataaaacgaa
     3601 gtgctccgct cgttcctacg gtcaaagcgg cgatcgtatc accaaaagca cctaacccta
     3661 gattagaaag cggcccgtct gcccctccca aaacaaatga tatatcattt ggcaaaccta
     3721 gttctgctgc tgtttttgat ggtagacctg ttacttgata ggtggaagga aagacttgcg
     3781 gcaattgctc tattcgaatg ttcaagtagt caagcacttc ttctgcccat tggaattcat
     3841 ggatatcaaa atatccagtt cctgaagctg ttccgtagtc gctgatcagt tcgccaaaca
     3901 atctataaaa gatatagtct ttgataccga caaaataagc agtttttttg aataattcag
     3961 gttggctgtt ttgcagccac ttgattttgt tcaatggtgt cattggatga atcggcgtcc
     4021 cagtctgccg ataaaattct ttgccaagca cggtatattt cagtttttct gcttcgtccg
     4081 ctgctcggtt atctgcccaa gtgatcattc gagataacgg tgttttctct tcatccaaca
     4141 atatcaggcc atgcatcgca gaggagaagg agacaaattg aatgattgtg tctgaaggca
     4201 actgtttaat tacttctttt ataacggtgc agacaccttg aaagatcaat tcctgatctt
     4261 gttctgccat cccttgtgtt tcttggatca aagggtagcg aaaataagcg gatgctgctt
     4321 cttgggcttc atctgtgaaa gcaactgctt tggtttgtgt ggttccgata tctaatccga
     4381 ttgaaacgtt cattttttca cctcaaattt tatgtatgta caaaaattta gtttaagctt
     4441 gcgtatcttg gttttgaaaa gggggagatg gcgtgttact ttattttatc attggttgct
     4501 gtatcggatc atttttatgt ttaaccgccc agcggattcc gttaggtcac tctattattt
     4561 atcctagatc tcattgtgtc aaatgctgtc gttcgctttc ttggtatgaa ttgattccga
     4621 ttctctcgat catcgtccaa cgatttcgct gccgctattg caggtgtcgg ttgcccttct
     4681 actatttgtt ggcagaagcc ttatgtggtg gtctttttgc ctggttcttt actttttctt
     4741 ccgcaagaaa tttttcgacg ttcatatgga tgctttcggc tttgctattt tcgttgatgg
     4801 atttatttta cttcatggtc cattctcaca cgctattctg ttcttgggca attttatgga
     4861 ttttttggtt gcaggccggc gtcttccaat ggcaaagtgt gctgttaacg tttatcgtag
     4921 gcggtgtgtg ccttcgctat ggtcaatcct atctcggagc tggcgatact ctgttgatgc
     4981 tttcttggag tggtggcttg tctctagaag agttgcttca gatcttgttt cttgctagtc
     5041 tgtttggaat atccgttttt tctgtttact ttatctgtca taaaaaaaag ttggaaaagc
     5101 tgcctttcat tccttttttg agcagtgcgt tgctgattgt ccttcatctt aggtattgaa
     5161 aaaaggttgt gacaaacgac ttgtcacaac ctctgatata tagtgttctc ttaccaaatc
     5221 cttgtgactt ggagctaggc gacaggttca caacctctcc actaaatggt gttcaatcag
     5281 ctgaccctca gtattagctc aaaatgctgg ttttacaacc tctgatatac ggtgttccat
     5341 aagtagcttt ttcggcttct tatttcttga cgccccaatg tcgacgaagg aaacctgcac
     5401 gtccagaacc ttctcgataa agattgtaat ggagcttgtt tttcttgtaa taatcctgat
     5461 ggtaatcttc tgcaggataa aattcgcttc ttggttcgat ttttgtcacg attggcttat
     5521 cgaatcgccc gctgtcttgc aatgcttgtt ttgaacgctc tgcaatattt cgttcttctt
     5581 catcattata gtagatcact ggacgataag aatcaccgcg atcagcgaac tgtcctaatg
     5641 cgtcagtcgg atcggtttgc tgccaataga tttccaccaa ctgatcataa gaaataactt
     5701 ctggatcata ttcgatctct actgcttctg tatgccctgt tgtgcctgtt gttacttgtt
     5761 cataagttgg atttggtaca tgcccgccag tataaccgga tgtcacagaa atgatccctg
     5821 gctgctcttc aaatggtttg accatacacc aaaagcatcc gcctgcgaaa actgcttttt
     5881 ttgtcatata aaatccccct tttttctcat cataccttga tttccaaaag gaggataacg
     5941 ttttgcttgt gacagaaaaa acggaaacta ttatatacgt gatacggctt ctgataaatg
     6001 ccaaaacgaa cgtacttgat caaaaacggt taaatcagga atcacatatt cagcagaagg
     6061 aagatttatg ttttcaccta ttcccaatac atgcatgtta ccagccaaag ctgcttgaca
     6121 accagcttct gaatcttcaa tgaccaagca tgcatttggc ggtacattca attgctgtgc
     6181 tcctttgaga aaaacttctg gatcaggttt tgcttttgat acttgcgtgc cgtcaataag
     6241 gacatcaaaa taacttgtca aattcgtttt ttctaaaatg aggcgtgcat ttttgcttgc
     6301 tgatcccaat ccgatcttga tgtttgtttt tttcaagtac tcaagaacag ctattgcacc
     6361 aggcaaaacg gatgtttcat ccattttttg aatcgcttga agatatagat tgtttttttg
     6421 ctcagcaaag gctgatttct ctttttctga gaatttctgt tccatttttc caaatgacag
     6481 caatcgctct agtgactcgg ttctggatat tcctttcagt tgctcgtttt ctttctcagt
     6541 gaatggaatg gacagctggt tagctaaaac cagccacgct tcgtaatgat aacgagctgt
     6601 gtctacaaga acgccatcta aatcgaatag tgctgctttg attttcatcg attacctcct
     6661 tgaaaatgaa cgacttgaag taaagatgcg atgtttttag ctgtgcgatg aagattatca
     6721 gtggcttgct gtaaagcttc ttgtaaaggc acagctgcag gaaggatcgg aaaaacggcc
     6781 gaaatgccaa catctgctgc tttttcgctt cctttaccaa cacttccgca aatagctatc
     6841 acaggaacgc cctctggtac tcttctagca atcccaactg gcgcttttcc ttttagactt
     6901 tgtccatcta gacgtccttc gcctacgatt acgaggtctg cttgctgtac ccgtttatcg
     6961 aactccagta aatccaatac gtagtcgatg ccagagacta ttttccctga agcaaatgct
     7021 aacagtcctg cacccattcc accaccagca ctactgcccg atgcctcgat tacttgaggg
     7081 gcaaatctca tataaaaatc atgcatggct tggtcagcca ctgctaattc attttctttc
     7141 aagccttttt gtggtccgaa aacaaaagaa gcgccttctt ttccgcataa aagattagta
     7201 acatcagtaa tgacagtgat tttggcttta atcagctcaa taggcacgtt cgttttatca
     7261 actgtagcaa ttttacctag attttctccc agtgcttcta ctttttgatt atcttgatcg
     7321 taaaaagcat aacctagacc ttctgccatg ccgatgccgc catcgttggt actacttccg
     7381 ccaacgccga tgatgatgtg tcgaacacct tgttgcaaca attgtcgtat cagttctcct
     7441 actcctcgcg tggataacct aagtggctgt tgttggttta gtgggacatc tttcaatccg
     7501 acgatttctg ccatttcaaa aatcgcagtt tgaccgtctg aagcgtactg gatttctttg
     7561 tttttgccaa atgcattagt aacgactgct gtttgccgct tcaaccttaa actgtctgct
     7621 aatgatgaaa ccgttccttc tcctccatcg cctataggta gcagatcata ttcagcctga
     7681 acaaaaactt cttgaaaacc tgattgaatg gcttgtgctg cctctaaggc tgtcagactt
     7741 tctttgaaag aatctggcgc aatcagaata cgcattattt tcccctccgt tccaagatca
     7801 gttctttttc cagctggtat tggtctccat tgacaaaaat aggtaatgtt tctttcgtct
     7861 ctttttttat tactatttgt ttttttgtca aaacaaatag caacctgtga tttttccaaa
     7921 taaaaggaaa agaaattttt tcccatgctg aaggaagtct tggagaaata gaaagagttc
     7981 cattcgttac tgtaatacct gaaaaaccaa atataactgc tagccatagt gctcctaatg
     8041 aagcagcatg aattccgtca tctgatgaat gcggatctgg tcctaaatca atcaggcacg
     8101 cttcttggaa taaatgatac gcccattcca tttcttggat acggcaagcc tctatggcgt
     8161 gaatcgcttt actcaacgat gagtcgtgga ttgttttgtt ttcatagtaa tgcaaattct
     8221 tagaaacgat tggttgagaa aaatactcag gtaataagta taacagcatg acaagatctg
     8281 cttgcttgct gacttggagt tcatttactt cgctacgggc ataatcaagt aaaatagact
     8341 ggctgccagg acgttctttg taagtggtca aatcaataac tggcttactg agatacgtgt
     8401 catcttgcgg aatcagctgt tctttgttag gcactggcaa atataactta tctaaaaaat
     8461 tttgacattc tttcaaaaaa agcggatcgc cagcttgata tttaacagct aaggccacat
     8521 tataatgcgc cagatagttc gtatatgcat tattgtcgat atattctgtg tattcatctg
     8581 gaccaatgac tctgtgtatc tctaaatgat catcgacttt tacagctctg ctcagccaaa
     8641 aagctgctgt ttcatgaatg agcgtttttc cctttgtttc taaaaacgat tgatcctctg
     8701 ttgcttggac atactcttga acggcataag caaggtctgc actgatatga tgttctgcgg
     8761 cagctgaagc aacttcttgt cttgttccgg ttttgatatt caatgcagca aatttgggtg
     8821 tctcttcctg accagtgtgt gcgctttccc atgggaaaag tgcaccttga tagtgataat
     8881 ctcttgcttt ttgccgtgcg ccttctaatc gatcataacg gtacagcagc aaattctttg
     8941 ctacttcagg aaactgatat aaaaagaaag gcaggataaa aatttccgta tcccagaaaa
     9001 catgcccttt ataaccttcg ccagttagtc ccttcgcccc tacactaaca cttggatcat
     9061 tgcggggagt cataatctgt aaatggtaag cagcgaagtc catcgctaac tgatcgaaag
     9121 aagaatcgcc ttggatcgtt atccgatgct gatgccaaaa atctgtccaa taagacatcg
     9181 actgctctaa aatttctgta taactaacgc ttttctgggc ttgggcaata cttttcttaa
     9241 acgggtctgt ttgctgaaga gaggtagaga gcacgatgat cttctccagc gtaaaggatg
     9301 tattttttct gatcttttct gaaaaggaag cggtgatccg gcgatttttt gcttggaata
     9361 cacctgcttt attgaattta gctgcaatcc caatatcaat tttactttct gttgtctggt
     9421 aggatgcact gatcaaatcg tcaccaaata ctcgcagttc tttttcaacc aactgctgtg
     9481 taccaaaatt ggtttgctgt ccatcgatac ccgttttcac atctacttgg cagtcttcgg
     9541 tcaacggctt gatgaccact ttcagggctg cctgatggag attattttga tcagcaacac
     9601 gacaagtaat gacttccact ttttttcctt gagcagtttc aatgagcgca tagcgtttaa
     9661 gctctcccat ttctaaatct agtgcccgct cgtatgcaag aactttcatc gtctcaagag
     9721 aaaaatgttc accgtcgatt tttatttccc atctgatgac atctgggaga ttcactaact
     9781 cggctgattc tgctcctaaa ggtcggttat atacaccagc tgcaaacatt ccccttactt
     9841 gattccgata gtcttcctcc tggcttcctc tgataccaaa aaatccattt ccaatggtca
     9901 tctgactcgc atacttattg atatactcta aatcaaactg tttttcttct actttcataa
     9961 ggtaataact tctcctgttt ctgccgaacg atataaattt tccaccagat tctggatgac
    10021 atatccttgc cagccatctg caatcatctg agtctggtca ccgagcgccc gattcacaaa
    10081 atgagccata cttagttcat gtgggtcatt aactggcgtg gtcttttgta aggaaaccag
    10141 ctttccatct acatcttcga atagttcgcc tggatacaga ttagcacctg cctgatcgcc
    10201 acaaaaagcc acattcagtt tgtcttctct gatattcaaa gcaaacgaag tattcagttg
    10261 gatgatcttg ccgttttcta attccagtgt gccaaaaatt gcatcttcta ctgtgaattt
    10321 gcttggatcc catttcccga atttccctga attttttttc gtaccaattt tttggaagct
    10381 gtacgcccga acgcttttta ctttaggaaa tccagtaata tacatcgctg tatcaagcat
    10441 gtgaatgcca taatcgatca aaggcccgcc tccttgaagt tctttgtcga taaacgttcc
    10501 ccagcctgga acaccagacc gacgaagcgc attcgcttct acgtaataaa tatccccgag
    10561 aacatcaaga tgattcttga tcattgttgc ttcgtcagaa aaacgatgct ggaatccata
    10621 agccaaaatc tgcccgtttt tatcggctgt ctctgccatc tttcttgctt gatcagctgt
    10681 catagctggt ggtttctcgc acatcactga agcattagct tcgagggcag ccaatacatg
    10741 ttcgaagtga aagcgattgg gtgtacagac actcacgata tcgggagaaa tctcattcag
    10801 catttgtgta actgatgtat acgcatgaga aaatcctgta cgtcggcaaa agtcttctgc
    10861 tcgctggctg actggatcaa ccacagcggc caattctaag acatctgatc ttttttgata
    10921 ataagcagca tgtacttttt ctgctacttg tccagcgcct atgattgcta ctttcttcat
    10981 ttatttctcc ctcctttgct tatttactta ttgttattct ttctctattc tttctctaaa
    11041 tcatttaaat gacgcgctgt ttccagacag tttttcaagt attgcaatga ttcctgataa
    11101 attttcactt cattttctcc tcgcaaacga cattcatata ccatataacc ttcatactca
    11161 ttttgaagta gcgtttgaaa atggcgagta aaatcaattg cgccagatcc tggttggtag
    11221 cgatgattat ctgctaaatg gatatgaccc acatactctt tttgcgtagt caatgctttt
    11281 gaaatatcat cttcttcaat attcatatga taaaaatcag cgatgatctg cacatgtttc
    11341 aattgattag agtcaatgta atgacgcgca tccgctaaca aattgatcat atggtcttgg
    11401 tatcggttca aaggctccaa ataaataacc gtacctgttt cttcggctac tttatctaaa
    11461 taacgaagtg attcgctgac tgctttatag tctccttctt tgcttcgggg tgaaaccatc
    11521 ggcggtaatc gataagtgaa cattccccaa gctgctggga taacgattcc tttgcctcca
    11581 acctcgctta gtgcacgcaa aatctcagca atttgacgta caccattaag acgccgttct
    11641 tcgataaagt caccgatcca cccatcgtag ccgttacaag ctgtcactac tgggatccct
    11701 gtttctttga ttgcctgttt gactgtgtcg atattttcta ttaacaactt accgtcgatt
    11761 tcgtattctt caaatccaca gtctttgata aatctaaatt tttctaaaat gtctcttggg
    11821 aaaaatgctt gatcttgcgt accgaatttc attgttctcc tcctcggact tgaacgccca
    11881 ttttgatact ttcttctggg tgctggtcta cataacgcat gtaagcttct gcgctttctg
    11941 caaatggcac aactggatca atgatttctt cacaatccaa gtaaccgttc atcaataatt
    12001 cccaacatgt gtcctcgatt cgtttacggt tccatcttgg gtaatctggg tttggttcac
    12061 tagatgctcg agcaaagacg atttttgcat tattgaaatg tgcttcgcga ccaagattaa
    12121 acccagctgg aaaaggttta gcaaaggcaa cataagaaat gatcccgcca tagccgatcc
    12181 ctcttaaaga agcttgtaat gcggcagcac tgccactcgt ttcaataact gaatctactc
    12241 cacgtttatc tgtcaattcc tttagacgaa aaccgacatc tgctttcata ggatctagcg
    12301 tgtaatcggc accgtatttt tctgctaatt ctctacgctg cgctatggga tcgatcccaa
    12361 cgatggtagt agcacctgct tttttagcta attgaatcgc aatttgcccg atagcgccta
    12421 aaccgaagat tgctacagca tcacctgctc gaacatgtgc ttcccggata ccacctaaag
    12481 cgaattgagc aggatcgtaa catactgcat ttttccattg atccaaccga tcaagtttac
    12541 gtagtttata attatcaaca ccgtttgtaa tcagcgtttc acgaatgggt ccataagtgc
    12601 agactaactc tcctttttga tactctgtca ccaaactgcc cgtttcttcg atcgtaccga
    12661 caatcatatt tcctaattga aattcgccaa ataccacacc gcgttggctt ccttcttctc
    12721 ttggcaagaa taggcgccat tcttctgaaa attcttcatc gataaatgga cttaatcctc
    12781 taaagtcgac aatttctgtt cctggttttg gagtagcgaa aattactcgg atacgtactt
    12841 cgttcttttc gatctcacgg tcttcgtaat ctttgaatcc ggctattctt ggttctaatg
    12901 caactaattt tttcatcttt tctttcctct ttcttttctt tttatccttt tacgccgcct
    12961 tcgactaaat cacctttaat aaatcgttca gataacgcat acataatgat gactggtaaa
    13021 gcggtcacca atgaagcggc catcattcgt ccccaaatgt agtctggtgt actgaataat
    13081 gtgttcaaac cgattggtaa agtaaatttt tcggaggtcg acaggaaaat cgaagcaaat
    13141 aaataatcat tccacgaaac cataaagcaa taaacgaaga cagaaatgat tccagaaata
    13201 gccaatggta taatgatccg aataatgatc tggatccggc taagaccatc gatcattgct
    13261 gcttcttcaa tatcagtcgg aatcgtgtca aaataacttt ttagcatgaa tatcgcagta
    13321 ggaagtgttt gaacgaccat cgtgataatc agtgctgtat tggtatcata caaacctaga
    13381 ccggaaatga ttttaaataa tggaacaacg atcaaaatac cggaaaacat ataaacggtg
    13441 taaaagctag cattgatttt cgagcgagct ttgaagcgta gttttgacaa agcgtatgcc
    13501 ccgctgatcc caatcactac tgcagttacg gaggctatca aggagatcac taaactattt
    13561 ttgaaataca tgatgaatgg aaaaatctca gcattgaaaa tatcctgata atgttcaaaa
    13621 gtccattctt tcggaaaaat agtttgtgtc gttgcaattg cttcttgtga atccttgaat
    13681 gatgtcatca gcatgatcaa aaatggaaac aaattgacag ctaatagcaa caaaatcgtc
    13741 gcgtaaaata agattttcct gctcttttta tttttcattt caagtccacc ttctttcgtg
    13801 agagcaagac gaccgctgca ataatgatga acaatagaat cgatatagct gctgcttttc
    13861 caagatcatt gaacgcaaag gctgtctgat acaaataaac gcctaagata ttgactttat
    13921 tcgtcagtag atagacatct gtaaacatat agaacatcca gatagatcgt aaaacaatca
    13981 cagttgctag tactggtttg attgccggca gtgtcacgat cttgaacttc tgccaagcat
    14041 ttgcaccgtc catttcagct gcttcataaa gcgttttgtc gactgtttgc aaaatagcca
    14101 aaaatgaaat aaacgcatac ggatagtatc tccagatagc aaacaaaacg accataaaga
    14161 agctagcaac tggctggtcg aaccacagcg gtgcttcgtt gaaaagctgc aacctatcta
    14221 ctaacagatc attgacgatt ccataactat tattgaacat atatttccaa gcaaaaatca
    14281 aagaaataga tggcgtaaca taacttaata aaatcagtga acgcaccgtt ttcctaaatc
    14341 tgaacttgcg gttaagcaaa atagctacgg ctaatcctaa agctgtactg cctgctacaa
    14401 ctaagaatgt gtaagcaact gttaaaccta aagaacgata aaattcacta tcacttaaaa
    14461 tttcaagata attgcttaat ccgataaaaa cggcgtctga gttaggattc agcggcatat
    14521 cgaaaaaact gatctgaata ttggaaagca ttggaatcag cactaaaaca gctaataaaa
    14581 gcatgcttgg tgctaaaagt cccatggcta attttaagtc agaatgtttc cttggacgat
    14641 tcattgaaac ctccccctga taatctgcca atagctatct acttttttcg aacaccattg
    14701 gcagaacttc ttgttatttc gtattttttt gtgcgtcagc taaagcttga tcgacatctt
    14761 cgtttccgac agttacttgg ttcactgctt ttccgatcac acctgagctt gtgatatctc
    14821 ccattttcgt gaaattcttt tccccgacta gtccaaagac ttgtacttca tcaaaagcac
    14881 tagcaatctc agatggcagc tcgccaaaag acttgatgac ttcattttct ttgtaaccat
    14941 ccagttcgac tacttgttta ttcactggtt gagcaccacc aggagacatc aagacccact
    15001 tttccatgtt tttcggttga gataagaaag ctgtgaatgc ttcagcagcc tctttttgtt
    15061 tctcgtctag accagaagaa atcgtcaatc cagatactgt gccatagaca gcttgatctt
    15121 tttgtgttgg gatagcaaaa ccgatatttt ctgagttccc cgcttcataa acagaaggca
    15181 aaatgtaagt tgaatagatt gccattggtg ctgttccatt catgaaagca tctttgattt
    15241 ctgtcacatc attagagcct ggcatcgtgt attgggataa gttttgataa aacgacaatg
    15301 cttctctcat cttttccgta tcgatcgtta cctcgccttc atcatccaaa acatttgctc
    15361 catttgacaa agcaaattga gagaacgctt gctcactcat tgttccttca gcagtaggca
    15421 tagcaattcc atattgttta ttctctttat ctgtgaaatg ttgagcaatt tttaaaatat
    15481 catcccaatt ttctggttct gaaaaaccag cttctgataa tttttctttg tcataccaaa
    15541 taccttgaac ccagccgcta attggtgcag caatatagct ttttccatct tctgaacgaa
    15601 caaggttttt ggctccgtca taataattat cttctccagc ttcctcgata acgttttgga
    15661 ctgccgtctg atcaatcagt tcatctttat ccatcacttt agcaaaatcc tgactgactt
    15721 ccattacttc tggtaattta ccggaacgag ctaacgtaac gatttttgta ttgaacgctg
    15781 attcttctac cggcacttgc ttgattttga tgtctggatt ttctttttca aaatcagcaa
    15841 ccagttcatt gatcacactt aatcgttcct gttcgacaga agagtgcatg aattgaatcg
    15901 ttgtctgttg tgattcgtta gagctttgac tttcaccaca agcagtaaga agaaatccag
    15961 tagctagcag acaggtagta acaataattt ttttcatttt tttccacctc ttattttatt
    16021 attttcagcc acatatattc atacggctgg aatatcaact ttcgttcttg actgcaatgg
    16081 ataatttgtc cagaaaacag gtctaggtaa tctccttttg gtaaaagata acctgctgat
    16141 tgattggaaa aattatggat aactaagact ttctcttcct tcgcttcgcg aataaaactg
    16201 ataaaagaat ctgctgagtc cagtaagtaa atcaaactat tcggatggaa ggctttttct
    16261 ttttttcgaa tagatagcat ctgggtcaat tcaccatata ctgctgttct taatggatct
    16321 gtctgcaact ctttctcaat atctgctaac aaaaattttt gccgattgat ttttcgattc
    16381 tctcctgttt tttctacgcc tttgtaatca tttcgacttc ccagcacact ttgaatataa
    16441 attgccggca cgcctggtaa cgttaccaaa aatgaatgag cagctaaaaa gcgtttgctt
    16501 ctccattccg tcgtatctgt tggagaactt aacgcatcta gatacgtagc gtttatctca
    16561 tatggactgg tcgttccatc tgggtttttc ttgtagttta ctcgggtacc ctctttcttc
    16621 agctgttcca ctaattcaag gatcgttttt tccggcagaa tacctcgtac cggattcaat
    16681 ccgataccgt catgggaggc taaaaaatta aaaaaagttg tttttttcga tgcgaaatcg
    16741 atggttttta accattcttt caacttactt gtattacccg aacgaatcgc ttgaagggtc
    16801 aaaggtggta gcgaaaactg atagaccatc tgtgcttcat tctcgccatt cccaaaataa
    16861 ctgatattat ctttgtgtgg cacatttgtc tcagtgatca ataccgttcc tggagctgct
    16921 tcatcagcga ttgcacggaa taattggatg attttatgcg tttctggcag gtgtatacaa
    16981 gaagttccta gttctttcca taaaaatccc acagcgtcca agcgaataaa tgaagcgcct
    17041 ttttctaagt aaaacaaaag aatatcgatc attttcaata aaacttctgg atttttaaaa
    17101 ttcaagtcta cttgatcttc gctaaaagtc gtccatacgt gtttttcttt cccgtttttg
    17161 tcaatgaacg ttgtaagtaa cgggctggtc cttggtctag tcacgctgct tgtatctgta
    17221 tccagtggca aatcaataaa aaaatctttg tacgtatcat tcaagtttaa atattcctga
    17281 aaccaagtac tttcggcaga catatgattg cagacaaagt cgaacattaa ccgtgtttcg
    17341 cttctcattt cttctatatc ttcccatgtg cccaaacggt cgttgattgc ctgataattt
    17401 acaacggaga aaccatcatc agatgtatat gggaaaaatg gcaagaaatg tacaactcta
    17461 aatgtatctg ccaaatactt ttgataaaat ttcttaaatg tacgcaaagt tggttgctgc
    17521 tcttcaaaaa attgatcccc ataagtaatc aagaaaatat cttcttcgtc ccaatgcagt
    17581 ttgcctgcct ggcctttctt ctgctgataa acactgactt tttgttcaat catagaggcg
    17641 gttttctcag acaaatctga gtcgcgataa atcgcttgta aaagttttgt atacatcgga
    17701 ttcccctttc ttttttggga gcgttcccaa cgatttgatc atattatgga aaacgcttac
    17761 tgtcaatagc atttttttaa ataattttga tatacacaaa aatacaaata agaaagctta
    17821 tgtatcaata attaatagct tcttaaaaaa atatggtctc tcatgatcat gcaagctatt
    17881 cctaaatttt caaaatcata gacaaaaaag aatgtctatt gtactatgga attattctaa
    17941 aacgctccca aaaaggaggt gcatataaaa ttgaaaccta ctatttatga cattgcgaaa
    18001 aaagctaatg tctcaaaatc aactgtctcc agagtattaa ataaccagcc aaatatctct
    18061 gataaagcaa aagcaagtgt acttcaagca atcaaagaat tgaattatga accgagcaaa
    18121 attgcaagag gactgtcagg aaatggtttt gacgcgattc tagcattatc tcatcgtact
    18181 tctcatacga cctatggcaa tccttttttt tcagatgtta ttcaatccat ttctgccgtt
    18241 tctgaacaaa ataacttcga cttgattcta caaacggcaa aaacaccaga agaagagcta
    18301 gaaaaatgcg cgaaaaagat ccaagaaaaa atgatccgag gaattattat tttaagctct
    18361 acgatggatg aaacacttct ttttgaattg gatcaataca atattcctat tgtggtaatc
    18421 ggaaaagtcg gaaacgacta cacgaacatc tactcagtgg acacggataa tttccaggat
    18481 agttacgatt tggtggaaac actgatcaag aatggacacc gtcatatcgg ctgtctccat
    18541 gctccgacag aagtttatgt atccaatgat cgtctcaatg gttaccgtca atgcttattt
    18601 gatcaccacc tagatatccg taatgaatgg attatagacg gcggctttcg cttagaagat
    18661 agcatggcag caatgaatga tttaatgaag ctacgagaaa gaccaactgc gattttttct
    18721 accgatgcat taaaatcatt gagtatttac aaagtggcag aacaatctgg aaagcagata
    18781 cctgatgatc tttcgatcgc aggattcagt gatcccttat tgtcctccct attttcgcca
    18841 cagttgactc ggatagacat tccgacaaat cagttaggaa tcaaagccac agaactttta
    18901 ttccaactaa tccaccacca ataccctgaa gaaaaaagaa tcattctttc aaccgagatt
    18961 actctcacag attcgatcca acaacaagaa atgtagaaca gaattataaa agaacagaaa
    19021 cactgaggat aagtcagtta ctctgttctt ttttcaatca ctcatccctc gttaccccct
    19081 cccggtattt tagcgaattt ttcttatttt ccatctgttt tagatagctg tggataacta
    19141 taatgtttgt gttaaactat tttttccaca gggaagaaaa aactattttt aaccacgttg
    19201 tgtataagtt tttatagttc gtttttttaa acacatttac ccttatttca atccatgtta
    19261 tttggggata cttatctcta aaacataaat agaataacag acatgtctac tggttttgtt
    19321 ttattcgagg aattattcaa taatagctag caaaatatta aaaataaccg ctctatcttc
    19381 ccatcaattt attttgcatt caaaaaatga tctatcttta cttttgttga ttgacttctc
    19441 ttggaagatg atcgttccga taccttaatt ggatacgaac cgatgttgtt aagatccttt
    19501 cccacttttc ttctagttag acaaattatt ttagacaaac caaaaaagag gttgtgacaa
    19561 taatttgtca caacctcttt tttaaaatat ctattttgtt tgattgttca ttgcgtcttc
    19621 tgcagccatt tgcgcttcaa tatctgaaat actgtagaca ttttcgcttg ctacaccgac
    19681 ttcttttggt tccatgtcac gataacgttg cataccagta ccagctggga tgatcttacc
    19741 tataataaca ttttctttca aaccaagaag aggatctttc ttaccgcgga tcgcagcatc
    19801 cgtcaagaca cgagttgttt cttggaatga tgcagcagat aagaaactat ttgtttctaa
    19861 tgatgctttt gtgataccta gtaagacagg tcgagaagtt gcaggaacgc ctcctgagac
    19921 tagcgtattg tagttttgat ctttaaagtc tgcgatgtct aataatgtac ctggtaagat
    19981 atctgtatca cctggatcca ttacacgaac tttacgcagc atttgacgaa ccattacttc
    20041 gatatgcttg tcgccgattt ctaccccttg catacggtaa acacgttgta cttcacgaag
    20101 caagtagttt tcaacagata atacatcgcg aacttgcaac aattgttttg gatcgattga
    20161 tccttctgtc aatggtgcac cacggtggat catatcgcct tctgctactt tcatacgtgc
    20221 tgtataagga acagtatacg tacgtgtatc tgtttttcct ttgattgtta cttctttggt
    20281 acgtgttgca ggatcttctg agatatcgat gacatcccct gttacttctg tgatcactgc
    20341 ttgccctttc ggattacgtg cttcaaagat ttcttggaca cgaggaagac cttgagtgat
    20401 atcgtctccg gcaaccccac ctgtatggaa tgtacgcatc gtcaactgag taccaggttc
    20461 accgattgat tgagcggcga tcgtaccaac tgcttctccg acttcaacgt cagagccagt
    20521 tgcaaggttg cgtccgtaac aatgtttgca gacaccatgt tttgtattac atgtgaagac
    20581 agaacggatt gtcactgttt cgacacctgc atcaacgatt tgttttgcga tgtcttcgga
    20641 aatcaattgg tttcctgcaa tgatcaattc atttgtttca ggatgacgta cttctttacg
    20701 ggtgtaacgt cccactaaac gatcttctaa tgattcgatg atttcatttc cctcgcggat
    20761 ggcttggatt tcaagacctc ggtcagtacc gcaatcttct tcacggatga ttacatcttg
    20821 ggccacgtca accagacgac gagtcaagta acctgaatcg gcagtcttca aggctgtatc
    20881 ggtcatccct ttacgagccc cgtgagttga gataaacatt tccaagacgg atagtccttc
    20941 acggaagttc gaaatgattg gcaattccat gattcgacca ttaggagcgg ccatcaaacc
    21001 acgcattccg gcaagctgtg taaagttgga aatgttacca cgagctccag aatcagacat
    21061 catgaagatt ggatttttcg cttccaaacc ttccatcagt ttttgttgga tgctgtcttt
    21121 tgcagcattc caaacagcaa tgacacgttc ataacgttcg tcatctgtga tcagaccacg
    21181 acggaattgt tttgtaatag tttcaacttg tttatgggca ttttcaatga tttcttgttt
    21241 ttcattcagt acactgatgt cagcgatccc tactgtgata ccggcatgtg tcgaatgttt
    21301 gtaccctaag tctttcatac ggtcaagcat cttagaagtt tctgttactt tgaatcgttt
    21361 gaagacttct gcgatgatgt ttcctaagtt tttcttcttg aacggtaata ctaattcttg
    21421 ctctttgata tgtgcaggaa tatcagtacc agcttcaaca aagtatttgt ccggtgtttg
    21481 gacggtcaag ttgaagtctg ttggttcatt cagataaggg aattcaggag gcatgatctc
    21541 gttgaagatg atcttaccaa ctgttgtgat catcgtacgt tctttttgcc attcagtaaa
    21601 tggtttttca cctaatgagt tagggttcac accgatacgt gaatgtaaat gaacatatcc
    21661 gttgcgccat gcgataacgg cttcgttttg atctctgaag accattcctt cgccttcgcg
    21721 tccttcttct tccattgtca agtagtagtt acctagaacc atatcttggg aaggagtaac
    21781 aactggttta ccatctttag ggttcaagat gttttgagca gctaacatca acatacgtgc
    21841 ttctgcttgt gcttcttcat tcaatggaac gtggacagcc atttggtctc catcgaaatc
    21901 ggcattataa gcttcacata caagtggatg caaacggatt gcgcgtcctt gtactaaaac
    21961 tggttcgaat gcttggatac caagtcggtg aagcgtaggt gcccggttca gcaatactgg
    22021 gtgttccttg attacgtctt ctaaaacatc ccatacttcg tcttcttgac gttcgatttt
    22081 acgtttcgca tttttgatgt ttgaagctag ttcgcgttga acaagctcac gcatcacgaa
    22141 tggtttgaat aattcaatgg ccatttcttt tggaagtcca cattggtaca tcttcaagaa
    22201 aggtccaaca acgataacgg aacgacctga gtagtcgaca cgtttaccta gcaagttttg
    22261 acggaaacga ccttgtttcc ctttcaacat atgagaaaga gatttcaatg ggcggttacc
    22321 tggtccagta actggacgtc cgcgacgacc gttatcgatc aatgcatcta ctgcttcttg
    22381 cagcatacgt ttttcatttt gaacgatgat tccaggtgca ttcaagtcta acaaacgttt
    22441 caaacggtta ttacggttga ttacacgacg gtaaagatca ttcaagtcac ttgttgcaaa
    22501 acgtccacct tctaattgaa ccattgggcg taaatctggt ggaataacag ggataacgtc
    22561 cataaccatc cagcttggtt gatttcctga agcgcggaat gcttctaaaa tatccaaacg
    22621 gcggattgca cgtgtacgtt tttgtccttg tgcagatttc aattcttctt tcaattcggc
    22681 tgcttctgca tctagatcaa cattatcaag taattgttta atcgcttctg cacccatagc
    22741 agcttggaat tcttgtccat attgttcacg tttttcgcga tattcgcgtt ctgtcaacaa
    22801 ttgttttttc tctaaattcg tatttcctgg ttcgatgact acatatgaag caaaataaat
    22861 gatttcttcc aaagcgcgtg ggctcatgtc taatacaaga cccatacgtg aaggaatccc
    22921 tttaaagtac cagatatgtg aaacaggagc tgctaattca atatgtccca tgcgttcacg
    22981 gcgaactttc gaacgagtaa cttccactcc gcatcgatca caaacgattc ctttataacg
    23041 gatacgtttg tattttccgc aggcacattc ccagtctttt gtaggaccaa aaatccgctc
    23101 acagaacaac ccttcgcgtt caggttttaa ggtacgatag ttaatcgtct caggtttttt
    23161 tacttcaccg taagaccagc ttctgatttt ttcgggagaa gccaaaccta tttgcatact
    23221 ttcgaattta tttacatcga tcaaaagggg ttccctccat ttcatttaat ggcaaaccgc
    23281 aagacggctg atcttcgacc gccttgcagc tcatagaccg gattagtcta aattatcttc
    23341 tgcggtttca aagttttgga tgacatcgtc tttttcatct tccacttgtt cagcagcttt
    23401 cttttctaat tctttggcag tttgctgttc ggcaaatttt gtcaaagcat caacagtgat
    23461 caaatcgtcg tcttcatcgt ccatgtcgcg aagttcgatc tctgtttctt cgatatccaa
    23521 gacacgcatg tccagaccta gtgattgcaa ttctttcacc aatacgcgga atgattccgg
    23581 tacacctggt tttggaattg gttcaccctt gacgattgct tcgtatgttt tcacacgtcc
    23641 gactacgtca tctgatttgt atgtcaagat ttcttgtaaa gtataagccg caccgtaagc
    23701 ttccagtgcc catacttcca tttcaccaaa acgttgtcca ccaaattgag ctttacctcc
    23761 caatggttgt tgtgtaacca atgagtaagg tccgattgaa cgtgcatgca atttgtcatc
    23821 gaccatgtga gcaagtttga tcatgtacat cacaccgaca gacacacgtc catcaaatgg
    23881 ttcacctgta cgtccgtcgt acagaacagt tttcgcatcg ctggccatac cggcttcacg
    23941 aactgtttcc catacgtctt catcacttgc accatcaaat actggcgttg ctacgtggat
    24001 acctaactga cgtgcagcca tacccaagtg taattccaat acttgcccga tgttcatacg
    24061 tgaaggcacc cctaaaggat tcagcatgat atcgattggt gttccatcag gtaggaatgg
    24121 catatcttct tccggcataa tacgggatac aaccccttta tttccgtgac gaccggccat
    24181 tttatcccct tcatggattt tacgtttttg aacgatataa acacgaacta acatgtttac
    24241 gcctggtgac aattcatctc cagcttcacg agtaaagatt ttcacatcgt gaacgatacc
    24301 gccgccgcca tgtggtacac gtaatgatgt atcgcggact tcacgagctt tttcgccaaa
    24361 gatcgcatgc aatagacgtt cttctgcaga taattctgtt accccttttg gcgtgacttt
    24421 ccctacaagc aaatcgccat cttggacttc tgcaccaata cggatgatac ccatttcgtc
    24481 taaatctttt aatgcgtctt ccccgacatt agggatttcg cgtgtgattt cttcaggtcc
    24541 taattttgta tcgcgggctt ctgattcata ttcttcaata tggatagaag tgtatacgtc
    24601 atcttttacc aaacgacggc tcatgatgat cgcatcttca tagttgtacc cttcccaagt
    24661 catgaaacca actaaaacgt tttggcctag agccatttcg ccttgttcca tagatggtcc
    24721 gtctgccaga gtatcccctt tttcgacttt ctcacccaaa tgaacgattg gacgttggtt
    24781 gtagcttgtt ccggagtttg aacgacggaa ttttgttaca tcgtatttat ctaatgcacc
    24841 gttgtcgcgg cgtacgcgga tttcagaagc atcaacgtat tcaacgacac cgtcatgttt
    24901 acataacaaa gcagctcctg agtcatgcgc tgatttgtat tccataccag taccaaccca
    24961 tggtgcttga ggattgatca acggcactgc ttgacgctgc atgttggcac ccatcaaggc
    25021 acggttggag tcatcgtttt ccaagaaagg aatacatgct gtcgcaacgg caactacttg
    25081 tttaggtgat acgtccatgt agtctacttt atcaatagat acttctaagt tttcactttg
    25141 agcacgagcc ataacaacgt cttgagcaaa tgttccatct tcattcaatg ggctgtttgc
    25201 ttgggctacg atgtaatggt cttcgatatc agctgtcaag tagtcgattt ggtcagtaac
    25261 acgacctgtt tcacggtcta cacgacgata aggtgtttcg ataaatccga atttatttac
    25321 tttcgcataa ctggacaaac tattgatcaa cccgatgttt gggccttcag gagtttcgat
    25381 cggacacata cggccatagt gggagtagtg aacgtcacga acttcataac cggcgcggtc
    25441 acgagtcaat ccgccaggtc ctaaggctga aagacgacgt ttgtgtgtca attcgccaag
    25501 agggtttgtt tgatccatga actgtgacaa ctgagaagaa ccaaagaatt ctttgatact
    25561 tgccacaact ggacggatat tgatcaattg ttgaggtgtc aatgtttcag tatcttggat
    25621 cgacattctt tcacgaacca cacgttccat acgtgctagc ccgatacgga attggttttg
    25681 taaaagttca ccgactgaac ggatacgacg atttcctaag tggtcgatat catcgacatt
    25741 gccgatacct tccatcaagt tcaagaaata gctcattgaa gcaatgatgt cggctggacg
    25801 aaccgttttt acagctgctt ctggatagcc gtttccgatc acgtttactt cacgttcagg
    25861 gtctcttggt gaaaatactt tgatgacttg gacagtcatt ggatctgtga ccacaccgtc
    25921 ttctgatgga tagtaagtca ctgaatttag gccattatca atgaaaggag ctagtgtttc
    25981 catgacttgg tgagtcagaa ctgttccttt ttcaacgatg atctcgcctg tttctggatc
    26041 aactaatgtt tcagctaatg tcagattcaa tagacgtgtt ttcaaatcta attttttgtt
    26101 tactttgtaa cgaccaacgt ttgctaagtc gtaacgtttt ggatcgaaga aacgagcatt
    26161 cagcaagtta cgtgagctgt ctgctgtttt tggttcgcct ggacgcaaac gttcgtaaac
    26221 gtctttcaaa ccttcttctg ttcttgagtc gcttgcgttt ttatgcaagt ctttttcgac
    26281 tgtattgcgt aatgtttcgc tgtcgccgaa gatttcaaag atcgtatcat cggaaccaaa
    26341 tcctaacgca cgaactaata cggttaaagg aatcttacgt gtacggtcga tacgtacata
    26401 agagatgtct tttgcatcag tttccatttc tagccaagct ccacggttag ggataacagt
    26461 agaaccgaat ccttcttttc cgtttttgtc gacttttcca tggaaataga cacctggtga
    26521 acggactaac tgagaaacga ttactcgctc tgccccgttg atgatgaatg ttccttgttc
    26581 tgtcattaat gggaaatcac cgaagaatac ttcttgtgct ttgatttcgc cagtctcgcg
    26641 gtttgtcaaa cgcaatgtta catgtaatgg tgcagagtag tttgcatcat gcgcacgagc
    26701 ttctgctact gtatactttg gttctttcaa ttcataatct acaaattcca aagatagatt
    26761 gccattgaag tcatcgattg gcaaaatgtc ttcaaacatt tctctaagtc cctcatctaa
    26821 gaaccattgg taagagtctg tttgaatttc gatcaaattt ggtaattcca acacttcact
    26881 gattcttgcg aaacttctac gttcgcgatg ttttccgtat tttactacgt gtccagccaa
    26941 gctcttcacc cctctaaaat gtcattcaaa aagactaccc gatcaattgt tacaataata
    27001 tttcaaatat tatttttagg taacaacaga cattttttgc ggcttttagg caaaaaaaaa
    27061 ccaaaaatag aacattctct tgaaacctta cttcgtgagc ttctactttt gtttccttat
    27121 attaaagccg aaacggacaa tatttcgttt ctgccttttc ttcatcagat agtttttgca
    27181 acttcatata ctacacttat tttctataaa agtcaacact tcctggcgtt tcttctgctt
    27241 tttcgcaaca ttttcttaat gttttcaatt ttatgttttc aattatctga caattcttaa
    27301 ttgacagacc tttttatctc ggttatactt tagaaaattc attttgtgga ggaacttcta
    27361 tgcgaaccaa catcaaccaa atcgcaccaa cacaactgaa taacttttac tttagctact
    27421 ttagataggt ttgtattcat catgatggat acaaacgtta aacgacagtt tgtatctatg
    27481 atggctaaag aactttgtat tgcagcaaaa agccacgtag acaaaatgct aagtggctga
    27541 gcagtgaaaa tacaaagtaa aagtatcaag cattctactt tttcaattta ttttccgact
    27601 tttcaatcca tttggacatt ttgtctgaat ggattttttg tttcccaaaa ttaaggagga
    27661 aaaagaaatg aaaaaaaata taatcatcgg attaggcgct gccctgctac tagctttagg
    27721 aggttgcgca caaaaagaca attccgcaga ggcatcagat ggacaaaaaa caattggcgt
    27781 attgcaactt gtacaacata actcgttaga tgctgcctat aaaggattca aagaaggatt
    27841 agcagaagaa gggtataaag aaggcgataa tctaacaatc gattatcaaa atgcccaaaa
    27901 caatcaagac aacctgaaaa gtatgagtga gaaactcgtc aaagaagatc ctgatttgct
    27961 acttggaatc gccactccag cagcacaatc tttattaaat gaaacaacca ctatcccgat
    28021 gactgtcaca gcggtcacag acttggaaca agcccattta gtcacatcca atgaaaaacc
    28081 tggcggcaat gtgactggca cgacagatat ggtgccaatc gaaaaacaaa tcggcttact
    28141 gttaaatatc gtgccagacg ccaaaacaat cggcatcatg tataacaacg gtgaagcaaa
    28201 ctctaagatc caagctgatt tggcagaaaa agaattgaaa aaagcgggcg tcaaagtcaa
    28261 agtactgaca gcaaattcca caaatgatgt ccaacaagtg acgaaaagtc tagctaaaga
    28321 tgtagatgga atctatattc ctactgataa cacgttcgca agtgcggcaa gtgttgtcgg
    28381 tgaagtcgca aaagaaacaa aaacacctgt cgttgctggc gcagttgatc aagtcaaaga
    28441 cggcggtcta gcaacagtag gaatcgacta cgaaagcttg ggtaaacaaa cagggatcat
    28501 ggcagcaaaa atcttagacg gcaagaatcc aagtgaactt cctgtcgaaa cagcgaatga
    28561 tttggaactg tatgtcaata aagacatggc caaagcatta ggcatcaatc cagacagcat
    28621 caaagaagct gaataaaaca caggaggatt ctcatgacaa atttgctctc tcttttctta
    28681 tcatctgcat cgcaaggggt cttatgggcc ctacttgcga ttggtgtata cgtcactttc
    28741 cgtatcttgg acattgccga cttgactgct gaaggaagct ttcctttagg tgcagggatc
    28801 gcggcagtaa gtattacaaa tggctatcat ccattgacag cttgtcttct aggcttttta
    28861 gggggtgcgg cagcagggct tgtttccgga ctgcttcaca caaaattgaa aattcccgcc
    28921 cttcttgcag ggatcatcac catgactggt ttatattctg tcacatcccg tgtgatgggt
    28981 gcgccgaaca tctctttatt gggtcagcca acgatctttc attgggtcga gtcattcggc
    29041 ataacaaaag acaacgctgt actgatcgtc ggtctgcttt ttgctttgat cgttgtatgt
    29101 ttacttgttt tgttcttacg tacagaaaca ggacttgcta ttcgtgcaac tggcgataat
    29161 ctggcaatga gcgaagcaaa cgggatcaac acagataata tgaagatcat cggttatatg
    29221 atttctaatg gatgcattgc tctttctggc tccttgcttg cgcaaaacaa cggctttgct
    29281 gatttaaatt ctggtattgg aacgatcgta attggacttg cttctattat catcgcagaa
    29341 gttttgttac gtaaccagcc actatttctc cgtctcttga ctgtcgtttt aggtgcggtc
    29401 atttatcgtt ttattttagc gttagtcttc gaactgaatg ttgaaccaag tgattcgaaa
    29461 cttgcttctg ctttcgtgct agtgatctgt ctttctttcc cgcagatcca gcaaaaattc
    29521 catttacctc aaaaaaagaa aggcggtagt gtcaaatgaa tgacgtctta acattggaaa
    29581 atctccatca tacctttgaa aaaggaacga tcaacgaaaa tcatgtcctg aaaggcatta
    29641 atctttcctt agatcccggc gacttcgtga cgatcatcgg tggaaatggg gctggtaaat
    29701 ctactttgct gaacagcatc gccggtacat tttctgtcga tcagggtcaa attttattga
    29761 atggaaaaaa tatcacgaaa aaatctgttg tcgaacgttc gaaatccatc agtcgcgtat
    29821 tccaagatcc taaactaggg acagccgttc gtttaacggt agaagaaaat cttgctcttg
    29881 ctatgaaacg aggaaaaaaa cgcggcttct tccgtggtgt caaaccacaa gaccgaacat
    29941 ttttcaaaga ccagctggtt cggctaaatt tggggttgga aaatcgttta tcctcagaaa
    30001 tcggtttgct gtctggagga caacgtcaag ctattacgtt attgatggcc acattacagc
    30061 gtccagaact gatcttatta gatgaacaca ctgcagcgct tgaccctgct acttccaaaa
    30121 cggtgatgga attgacagaa aagctgatca ctgagcagaa tctcactgct tttatggtca
    30181 ctcatgacat ggaagacgcg atccgctatg ggagtcgact gatcatgctg catcaaggaa
    30241 ggatcgtcgt agatatccag caagaggaaa aacagcagct cacagtgccc caattattag
    30301 cgttgttcaa acaaaacagc ggcacggcat taaaagacga tcaagtactt ttatcttcat
    30361 aatcagaaag aggatgggac aaaaaatgaa cgcccaccct cttctttttt ctctttcctt
    30421 ctttcgtttt tgctttatgt atcaaccgtt gtttcttcga aaactatttt gttctctcct
    30481 tcttcctgat agaatggatg ggaaaggagc ttgatgccat gacaacgaaa gacaaggtac
    30541 ttcaattatt gaaaaagaca ccagattttc tttccggaga aaagttggca cagcaactgg
    30601 aagtttcccg gacaagtatt tggaaagcca tcaaagaatt ggaaaaggca ggctatcgtt
    30661 ttgagcacgg gcctacaggg tatcgttacc tgccctctga cgtactagat gcaaaagaaa
    30721 tccagcaaga gctacggtcg tttattcctg atttgaaagt acaaacgatg ttgacttctg
    30781 aatctacgat gaaagatgcc aaactggcag cagctcaagg agattctgtt tcgaagttat
    30841 ttgttgctga tacacaagaa caagctcgtg gacgttttgg acggcctttt ttcgctgaac
    30901 ctggacgagg aatctacatg agtctcttgc tccatccaga caaacggttt gatgaattgc
    30961 ctcaatatac gatcatcgca gccagtgcct gtgtgaaagc cattgaatct gtaaccggtc
    31021 gttctgcttc gatcaaatgg gtcaatgata tctacatgga tggaaaaaaa gtctgtggga
    31081 ttttatctga agcagtcagt gatatggaaa gcggcaggat cagtcatatc atcattggga
    31141 tcgggcttaa tttttctgtt cctcaaaatc attttccagc tgatctccaa gaaaaagcca
    31201 tctctgtctt tcccgacgga caagctagtg tcacaaggaa tcagctaatc agcaaaatct
    31261 ggcaagaatt gttcacttat ctttctcacc cagaaaaagc gttggacttc tataaagaaa
    31321 aatcattcgt attgggaaaa accgtgtcgt tcaatcaaaa aggaacgact tatgtcggtc
    31381 ttgcaaaaga aatcggtgat catggcgaac tgatcgtcca aacaaatgaa cagaagatga
    31441 gtttgtcttc tggcgaaatc agtctcacat ctatccgtac gcaataatta tcagatttca
    31501 caaaaggaca cctgatctca tacagtgtcc ttttttctga attctcttca aaagactctc
    31561 cttattctat cagctgacag tttctctatc ctctagaaaa aggtattcta aaaatatcta
    31621 taaagcatgg tacactttct ttaaatgaat caatgcaaaa aacaagagag tcatcagttt
    31681 acacgatcaa aagagatagc aggccgcttc ctttttcaat cttatagata gaccatgggt
    31741 aacgataaaa acaaaaaaga acttgcgact tattttgccg taagttcttt tttagctagt
    31801 gccttaatct cttaatccca acaaacgtct cgcatagatc acctgaccag agtgcatagc
    31861 agcatcatca ataatagaaa cgagacgcat tcctcgtgtg actggtggtg tccagttctc
    31921 gtcaattact tcatctagct cggaaggttc cagccttctt aagtaagaaa tagcaaaatc
    31981 agcagctcct cgcaagtatc ctagcagatc ttctatttga ttagcttgga tctgctttgc
    32041 ttgttccaga ctatgcttcc aatcttgttc gttatctgct atcgtcatcg gaaaccggct
    32101 ttcccagtct tgactatgcc aaattggaga agttcctgct aaatcagcaa tctggaaatc
    32161 taattctcga gcggtatgcc aagccagcca agtcattgat ttgatctgcg gggctctatc
    32221 ttccactgga aaacgattgg cctcctcaat agagacgcct tcaaacaaac gttcaaaccg
    32281 atcgtatgcc cgatccacac tctcgcatag catatctaaa taatccattt attttcctcc
    32341 tttttattta tcctacttac tttttcctaa ttctaggata cctacttata tgttactaca
    32401 cacccttatt ttttacactt taacagtcac catcatcaat agtttggaga ttgaaagaaa
    32461 aagatagtgt cgaactatca attgaacaag aagcaacaat atgaacgttt cattaatatt
    32521 ctttaaataa acaatgattt ttcaaacgaa cagtttaaga ctaggagaaa aagagtataa
    32581 taggaaaatg tttgaaagga aaggaagggg aaaaatgaaa aaaagggcaa gtttactagt
    32641 cctagttgct ttgttgttca atatcttttc gtatggtgtg gccgtttcag cggaaacata
    32701 cattcctaaa gaccctggga atatttataa tgatctaact ggtaactatg gcttgttagg
    32761 aattgctagt caattccacg tatttgctaa aaataagaca actgtgaatg cacatacaga
    32821 cggaaatata gcaacacatg aacttgatgc aaaaaataat tttggtacag agcttcatga
    32881 aggtttattg aaccaagaaa taaattatat acaaaaaact gattctttcg ctaattcttc
    32941 aggcatacct acaggtagca caagaatgaa taaatttgtc gttggcagta acgtggatgt
    33001 agcacttcaa gatggtcatc ctacagtcaa tggatccagg cttgatcact taactccaga
    33061 tgatttttat aaagatcgtg ctgggcatga atatattgat ttcgagcagg aatttgctaa
    33121 attgaatgta gcagcaaatg atcttgcaac gatcactcca gctaaaacat atactgctgc
    33181 agattttcca gacatgaaca atcggacaat cgatttaact gatttatcag ataccgggtt
    33241 tttattggta aatatcgatg cagaagtgtt aacgatgaat acgccacttc aaatcattaa
    33301 tccaaatgat caagtagttg tatttaatgt catcaacagt tcttctgcat tgaacgtaca
    33361 gtcaccaatc aaatacaatg atcgttcaaa tcatgaaaca gaagattttt cggatgcgaa
    33421 tatctcttgg aattttggaa atgagatgac tgatttgaca atcagtgctc cttttcaagg
    33481 aacaatcctt gcgcctaatg ctactatccg cattactcaa aaccaagacg gaacgattat
    33541 aggaacaaat gtgattttag atgcagctac caatcgttgg gatcctaacg aaatatttat
    33601 cagcgacaat ggtacagata cgacagacac aagcgacagt acagatacat ctaccacaag
    33661 tgatagctca gattcatcaa cttcaagcga cagtacagat actacctcta ccacaagtga
    33721 tagcacggat acatcagctt caagcgacag tacagatact acct
//
LOCUS       DACDIG010000026        31735 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.026, whole genome shotgun sequence.
ACCESSION   DACDIG010000026 DACDIG010000000
VERSION     DACDIG010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.026"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            337..1947
                     /locus_tag="IV247_001310"
     CDS             337..1947
                     /locus_tag="IV247_001310"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAP8777389.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            2239..3108
                     /locus_tag="IV247_001311"
     CDS             2239..3108
                     /locus_tag="IV247_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAP8777390.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            3273..4544
                     /locus_tag="IV247_001312"
     CDS             3273..4544
                     /locus_tag="IV247_001312"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP8777391.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            4541..5836
                     /locus_tag="IV247_001313"
     CDS             4541..5836
                     /locus_tag="IV247_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAP8777392.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            5935..6198
                     /locus_tag="IV247_001314"
     CDS             5935..6198
                     /locus_tag="IV247_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAP8777393.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            6643..7092
                     /locus_tag="IV247_001315"
     CDS             6643..7092
                     /locus_tag="IV247_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8777394.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            7149..8567
                     /locus_tag="IV247_001316"
     CDS             7149..8567
                     /locus_tag="IV247_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8777395.1"
                     /translation="MKIVGVTACPTGIAHTYMSVEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            8567..9427
                     /locus_tag="IV247_001317"
     CDS             8567..9427
                     /locus_tag="IV247_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAP8777396.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            9601..10374
                     /locus_tag="IV247_001318"
     CDS             9601..10374
                     /locus_tag="IV247_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8777397.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            10521..10886
                     /locus_tag="IV247_001319"
     CDS             10521..10886
                     /locus_tag="IV247_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAP8777398.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(11034..12242)
                     /locus_tag="IV247_001320"
     CDS             complement(11034..12242)
                     /locus_tag="IV247_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8777399.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(12261..12539)
                     /locus_tag="IV247_001321"
     CDS             complement(12261..12539)
                     /locus_tag="IV247_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777400.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            12732..13388
                     /locus_tag="IV247_001322"
     CDS             12732..13388
                     /locus_tag="IV247_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP8777401.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(13830..14627)
                     /locus_tag="IV247_001323"
     CDS             complement(13830..14627)
                     /locus_tag="IV247_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAP8777402.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(14624..15808)
                     /locus_tag="IV247_001324"
     CDS             complement(14624..15808)
                     /locus_tag="IV247_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP8777403.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(15833..17080)
                     /locus_tag="IV247_001325"
     CDS             complement(15833..17080)
                     /locus_tag="IV247_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAP8777404.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            17295..19040
                     /gene="dnaX"
                     /locus_tag="IV247_001326"
     CDS             17295..19040
                     /gene="dnaX"
                     /locus_tag="IV247_001326"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAP8777405.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            19060..19374
                     /locus_tag="IV247_001327"
     CDS             19060..19374
                     /locus_tag="IV247_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAP8777406.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            19399..19995
                     /gene="recR"
                     /locus_tag="IV247_001328"
     CDS             19399..19995
                     /gene="recR"
                     /locus_tag="IV247_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAP8777407.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            20059..20703
                     /locus_tag="IV247_001329"
     CDS             20059..20703
                     /locus_tag="IV247_001329"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAP8777408.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            20718..21047
                     /locus_tag="IV247_001330"
     CDS             20718..21047
                     /locus_tag="IV247_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777409.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            21061..21999
                     /gene="holB"
                     /locus_tag="IV247_001331"
     CDS             21061..21999
                     /gene="holB"
                     /locus_tag="IV247_001331"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAP8777410.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            22035..22859
                     /locus_tag="IV247_001332"
     CDS             22035..22859
                     /locus_tag="IV247_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAP8777411.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            22852..23199
                     /locus_tag="IV247_001333"
     CDS             22852..23199
                     /locus_tag="IV247_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAP8777412.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            23268..24140
                     /gene="rsmI"
                     /locus_tag="IV247_001334"
     CDS             23268..24140
                     /gene="rsmI"
                     /locus_tag="IV247_001334"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAP8777413.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            24249..25370
                     /gene="dinB"
                     /locus_tag="IV247_001335"
     CDS             24249..25370
                     /gene="dinB"
                     /locus_tag="IV247_001335"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAP8777414.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(25424..26026)
                     /gene="nrdG"
                     /locus_tag="IV247_001336"
     CDS             complement(25424..26026)
                     /gene="nrdG"
                     /locus_tag="IV247_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAP8777415.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(26341..28530)
                     /gene="nrdD"
                     /locus_tag="IV247_001337"
     CDS             complement(26341..28530)
                     /gene="nrdD"
                     /locus_tag="IV247_001337"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAP8777416.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            28856..29623
                     /locus_tag="IV247_001338"
     CDS             28856..29623
                     /locus_tag="IV247_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8777417.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            29613..31655
                     /locus_tag="IV247_001339"
     CDS             29613..31655
                     /locus_tag="IV247_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8777418.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT        10199 a         5667 c         6651 g         9218 t
ORIGIN      
        1 taaaattttt cagtaacgat tttctgattt gcttaggttt aacgttagct atgtttatat
       61 caagcttatt atgtgggtta aatttttttt gcactcataa aggacttaag aaataatttc
      121 tcaaaggata tcgaaaaaga aaaaattgta agcggatagt attcatttga gtacttgact
      181 ttcccttagt cagcttgtat tatacacctt gggcaccctt ttagagggtc agaagtttac
      241 agaatttcgg ctccctattc ttattgaaga atagggagct ttgtttattg tctgagactt
      301 ttcctgcaca taaattaaaa aggagttttt gaacttatga caaaatatat ttttgtgacc
      361 ggcggtgtcg tttcatcaat cggaaaaggg attgttgcag cttctttagg acgcttattg
      421 aaaaaccgcg gcttaaaagt aactatccaa aaatttgatc catacatcaa cgtggatccg
      481 gggacgatga gcccatatca gcacggagaa gtattcgtaa cagatgatgg tgctgaaaca
      541 gatttggatc tgggccacta cgaacgtttt attgacatca atttgaacaa atactccaac
      601 gtgacgacag gaaaaatcta ttctgaagtt ttgcgtaaag aacgtaaggg cgaatacttg
      661 ggagcaactg ttcaagttat tcctcatatt acaaatgaga tcaaagaaaa aatcatgcga
      721 gcagctaaaa tgactgattc agatgtgatc attacagaag tagggggaac agtaggggat
      781 atagaatctt tgccattcct tgaagcactt cgtcaaatga aagcagatgt cggtacagat
      841 aatgttatgt atatccatac aacattgatt ccttacttga aagctgctgg agaaatgaaa
      901 actaaaccaa cgcagcacag tgtaaaagag ctacgtggtt taggtatcca accgaatatt
      961 ttggtggtac gtacagagca acctgtttct caaagcgtga aaaacaaatt ggcacaattt
     1021 tgtgatgtcg aaccagaagc agttatcgag tcgccagatg tagatacact ttactccatc
     1081 ccattagttt tacaagcaca aggaatggat caaattgttt gcgaccattt aaaactagat
     1141 acaccagaag cagacatgac agaatggaaa gaattagaag aacgtgtctt gaacttgaag
     1201 aaaaaagtac ggattgcgct tgtcggtaaa tatgtcgagt taccagatgc atatatctca
     1261 gtagttgaag cattgaaaca ttctggtttc gcctataatt cagacatcga gatcgattgg
     1321 atcaaagcac aagaattaac gagagaaaat gtagaagaac gtttgaaaga cgcagatgga
     1381 atcttggttc ctggtggatt tggtgatcgc ggagtagaag ggaaaatcga agcgattcgt
     1441 tatgctcgcg aaaatgacgt tccgttttta ggtatttgtc ttggtatgca gatggcttgt
     1501 gtcgaatttg cacgtaacgt cgttggatta gaagatgcgt catcagctga aacgaatcca
     1561 gatacagcaa acaatattat tgatttaatg gctgatcagg aaaacatcga gaatttgggt
     1621 ggaactttac gccttggatt atacccatgt aaaatcaaaa aaggaacgaa gacggctgaa
     1681 gcatatgatg gagccgatgt tgtgcaagaa cgtcaccgtc accgctatga gttcaataac
     1741 aaatatcgtc aattgtttga agaacaaggg ctagtctttt caggtgtctc accagataac
     1801 cgtttagtag aaatcgtaga gctgccagag aaaaaattct ttgtgggatg ccaattccac
     1861 ccagaattga tttctcgtcc aaatcgcccg caaaaattaa tcaaagcatt tgtcggagca
     1921 tcattagcag atcacgaaac aaaataattt ttgaacttga tagaaagaga aaagtagcaa
     1981 gagaagggga taagcaattt tcttgtcact tttctctttt tctgatttgt tattatttgt
     2041 aggaaaaaag atagaaagca aaagactttt ttcgattaaa gcgctattgg atattttatt
     2101 ttttgcaaaa aacttcttga aaattttcat tttcattgat tttagtagaa aactaccgaa
     2161 atgtttggta aaatagggtc gttggttaag aattgtctta acaaatttag ttttacaaaa
     2221 cttaggagga attttattat gccagtagta tcaggagctg aatttttaaa agctgctcgt
     2281 aaaggcggtt acgctgtcgg cggattcaac acaaacaact tggaatggac tcaagcaatc
     2341 cttgaagcgg ctgaagcaaa acaagcacca gtacttatcc aaacatcaat gggtgcagct
     2401 aaatacatgg gtggttacaa aatctgtaaa gatttaatca ctgatttagt tgaagcaatg
     2461 aacatcactg ttccagttgc tatccactta gaccatggtg attacgaagc tgctttggaa
     2521 tgtatcgaat taggctatac ttcagtaatg ttcgatggtt cacatctacc atttgaagaa
     2581 aacttgaaat tagctaaaga cgttgttgaa aaagcacatg caaaaggtat ctcagttgaa
     2641 tgtgaagttg gttcaatcgg cggagaagaa gacggaatca tcggtaacgg cgaattagct
     2701 gatattgaag aatgcaaaca aatggttgct acaggtatcg actacttagc ttgcggtatc
     2761 ggtaacatcc atggtcaata tccagaaaac tggaaaggat tagcttttga ccacttacaa
     2821 gcaattgctg atgctgtagg tccagacatg cctttagtat tacatggcgg atcaggtatt
     2881 cctcaagatc aaatcgaaaa agctatctca atgggtatct caaaagtaaa tgttaacact
     2941 gaattccaat tatcatttgc tgcagctact cgtaaatata tcgaagctgg taaagataaa
     3001 gaaggtaaag gattcgaccc tcgtaaatta ttagctccag gtaaagcagc aatcgttgaa
     3061 gatgctaaat ctcatatcga ctggttcggt tcagctaaca aagcgtaagc atccacataa
     3121 cagatacgat atataggaat ggagaacgac ttttgaagtc gctctccatt cttttttact
     3181 taaaacggca gaccatgctc gtttcttttt tttatctatg ataaaataca agcatatcca
     3241 tgaaaaaaga ttaataaaca aggtggaaac agatgaaaaa aatcgtaatc aaaggaaacc
     3301 gacctttagc tggtgaagta acgatcagcg gtgcaaaaaa tagtgcagtc gcattgattc
     3361 cagctgcaat cttagctgat tccccagtga tcttggaagg tgtgccggac atacaagatg
     3421 ttcattcatt aattgaaatt ttagagatca tgggcgcaac tgtccatttt tccaacaaca
     3481 tactagaaat cgatcctcga gaagtcgtat cgattccgat gccgcacgga aaaatcaata
     3541 gtttgcgtgc ttcttattat tttatgggaa cactgcttgg taaatttggc gaagcggtag
     3601 taggcctgcc aggtggctgt tatttaggcc ctagaccgat tgacctacac gtcaaaggat
     3661 ttgaagctct aggtgctgaa gtgacaaatg aacatggtgc catgtattta cggacagaaa
     3721 ataaaggatt gcgtggcaat cggattttca tggacgtggt ttcagtcgga gcaacgatca
     3781 atgtgatgtt agctgctgta aaagcaaaag gacagacagt aattgaaaac gctgcacgag
     3841 aaccggaaat catagatgtg gcaactttac tgaataatat gggggcaaaa gttcgaggag
     3901 cgggtacaga tgtgatccgt atcgaaggag tcgaaacatt acatggctgt cggcatttca
     3961 tgattcctga ccgcatcgaa gcaggaacct atctagcatt agcagctgca gtaggaaatg
     4021 gtatcaaagt gaaaaatgtg atctttgaac atttggaaag ctttatagca aaattgcagg
     4081 aaattggtgt ccatatgaaa atcagcgaag atgaaatcga agtttacccc tcgaaagaat
     4141 taaaacccgc aaacatcatg acctatcctt atccaggatt tgccacagat ctgcagcagc
     4201 cacttactgc gttactattg atgactacag gcacttcaga aatcatcgat acgatctatg
     4261 cgaaacgtgt gaaccatgtg ccggaactag cacgaatggg cgctgatatt accgtggaag
     4321 gaaatatgat catcgtgaat ggtccaaata aacttcacgg cacagaagtc gtggcttctg
     4381 atcttcgggc aggtgcttgt ttagtgattg ctggactatt ggcagaaggg acaacgacga
     4441 tttacaatgt agattatatc ttacgcggtt atgatcatat tatcgagaaa ctaacagcct
     4501 taggggcatc aattgaaatg atagaggaag tgggagctga atgagcgatt atttgacaat
     4561 ggcagaacta gagaacaaaa cgttaaaaga agtttatagt tatgccaaag aatttaaaat
     4621 tccttattac agtaagatga ataaaaagga attatctcta gctgtgattc gtgcacaagc
     4681 agagaaacaa ggattttatt atatggaagg aatattggat atcgttggtc aagatggtta
     4741 cggtttttta cggccaatca attatggacc aagtgcagaa gatatctata tttctgcctc
     4801 acaaatcaga cgattcagtt tgagaaatgg ggacaaagtg gcaggaaaag cccgtccacc
     4861 taaagaatcg gaacgttatt atggtttgat gcacgtggaa agcgttaatg gaaaagatcc
     4921 ggaagaagca aaagaacgtc cgcattttcc agccttaaca cctctttatc cgcaaagaca
     4981 gcttcaactg gaaacaacac cgcaaagact atctacccga atgatcgata tcttttctcc
     5041 aatcggtttt ggtcagcgtg gattgatcgt cgcaccgcca aaagcaggga aaacttctgt
     5101 attaaaagaa atcgcaaatg gtatcaccga aaactatccg gaagtagaat taatcgttct
     5161 actgattgat gaacgaccgg aagaagtaac ggatctagaa cgaagcgtga aaggcgacgt
     5221 ggtatcttct acatttgatc tgcagccgca aaaccatact cgagtttctg aattagtatt
     5281 ggaacgcgca atgcgtttag tcgaagataa acgagatgtc gttatcttga tggatagtat
     5341 tactagactt gctcgggcct ataacttagt cgttccacca agcggacgta cattgagtgg
     5401 cgggattgac ccagccgcat tgtataaacc taagaaattt ttcggggcag caagaaacat
     5461 cgaagaaggt ggcagtttga cgattttagc gaccgcttta gtcgatacag gaagccgtat
     5521 ggatgacgtg atctatgaag aattcaaagg aactggaaat ctagaattac aactttcacg
     5581 tgaattatct gaacgtcgga tcttcccagc catcgatatc aaaaaatcag gtacccgaaa
     5641 agaagaatta ttgatgccaa gtgaagaatt agaagaaatt tggaaacttc gaaaaaatat
     5701 gactggagat tcattagaat atacggaaca actgatccgc tttttgcgta aaacaaaaaa
     5761 caatcagcaa tttttcaaag aatttcaaga tgtctccttt ggtaaaaaaa atccgaccag
     5821 aaatctgaaa agataattgc aaccttgact gaaacatgtt aagatgttaa tgttgtaaat
     5881 cgacaaatca actctggttc agcaaatgga gcagggcaaa ggagcgaaaa aatcatgaaa
     5941 gaaaacatcc atccagatta ccacccagta gtattcatgg attcaacaac tggtttcaaa
     6001 ttcttgtcag gttcaacaaa aacttcacaa gaaacagttg aatgggaaga cggtaataca
     6061 tacccagtca tccgtgtcga agtaacttct gactcacatc cattctacac tggacgtcaa
     6121 aaattcacac aagcagacgg acgtgtggac cgtttcaaca aaaaatacgg tctcaaagac
     6181 gcaaacgctg cggaataata aaaacgctga taactcaacg tttagcagac tatctgatag
     6241 ggatagtctg tttttttatg ttttttgcca atttaaaact tcgtttgatg ctgaaaaagt
     6301 cgtaggatca taatgcacaa aacaatgaaa aactagtata tagttttaat tatccacttg
     6361 ataaaaacac cttatattaa cgaaatgaag aggggttatt tcgaagtaaa aaaccattta
     6421 cctaaaatta gaagctaagc tttcctttca aggaaggctt tttgttttgt tatagacttg
     6481 cgattgtttg tgagctagat cgttcatttt aatgacttat acacgcggat gatgggtctt
     6541 tttcctctgt tcattcataa tattgaacgg tacagctagc gatgctatac tgacctcagt
     6601 tccaagaaaa cgttaacatt ttttgtgggg gagaggtttt ttatgaggtt tgatcaattg
     6661 atcgcagaag aactgattgt tttcgatcca tcgatagatt caaaaggatc attatttgag
     6721 cgaatagggg atgtgttgaa aaaaacaaat aaggtaagaa atgttcaaaa agtcgttaag
     6781 gcactattca aacgagagga ggagatctcc acaggaatag aagatggatt tgggattccc
     6841 catgcaaaaa gcaaatatgt gacaaaacca gcactgtgtt tttttcatac agccaaatta
     6901 acggactata gcgggataga tgaaatgcca attgaatata tttttgccat cgtagttcct
     6961 gaaaaatcag cagatatgca tctagagatt ctcagcacac tctccagacg attgatgaat
     7021 gcagatttta gaaagggaat aaaagaagcc actacgccag cagatatcat cagacagatt
     7081 agtcaagaat gaaaatagta gagtctttgt ccgagtatcg atagtgaaga aaaaggagga
     7141 aaataatcat gaaaatcgtt ggtgtaactg cttgcccgac tggaattgct catacttata
     7201 tgtcggtaga aaaattagaa gtaacagcaa gaaatctaaa ccatcaagca aaattcgaaa
     7261 cacaaggtgt aaaaacagaa aatcagttat ctgaacaaga aatcaaagag gcagatgcaa
     7321 tcattcttgc agtagataaa gaaatcgaat tggaccgatt tgccgggaaa aaagtaaagc
     7381 gtgtctctac aagccgagca atcaaagaac cgcaagtagt aatagacgaa gcattgagag
     7441 atattggcgt attcgttgtg tctaatgaaa aagaaccagc tgcaaatgag aaaaagccga
     7501 caatctataa tcatttcatg aatggtgtga attacatgct tccatttgtg atcgcaggcg
     7561 gaatcatcat cgcactgagt tttgcctttg ggattacagc ggctgatccc aactcagcag
     7621 actataatgt gttagcagct gcattttcaa ggatcggagg agatacagcg tttgccatga
     7681 tggttcctgc gttaggcgcg gggattgcta catcgattgc tggaaaagca ggttttgccc
     7741 caggaattgt tgccggatta ctggcctcta caggtggttc aggttttctt ggcggaatga
     7801 ttggtggcgt gctagcaggg tatatctgtg attttctggc aaataaaatt agcgtaaaaa
     7861 aagaattttc tgctatttat cagctgattg tcgtcccctt tttatcgatc ttaattattg
     7921 ggcttttgat ggtatttgtg attgaacaac cgatcacatg ggtgttggat gctttgacaa
     7981 attggctgaa tagcttggga aatacatccg ggctattgtt cggattactt atcgggatca
     8041 tgatggcagc tgatatgggt gggccgatca ataaatcgac ttcgactttc tcgatcggtt
     8101 tgatgtcagc tggagtaaat gcaccgattg ctgcttgtat ggctgcagga atgacacctc
     8161 ccctaggtat tgctttagcc actttattat tcaaacataa atttaccaaa gaagaacgga
     8221 cttcaggtca atcatgctgg attttaggcg cttcgtatat tacagaaggt gctataccgt
     8281 ttgcggtatc tgacccgatc cgcgtgatac ctagtttgat gacaggttca gcagtcgctg
     8341 cagggatttc catggcagta ggagttactt caatggcacc tcatggaggg atttgggtga
     8401 tgctaattcc aaatgttatg aacaatttac ctatgtatgt cttggcaatt gcatcaggta
     8461 cagtcactac agcctttatg cttggactat tgaaaaaaac agtgaaagag aaaaaacaag
     8521 acaagtggac gaaaaatgaa aatctaacaa atgaggtgga agcgtaatgt tatatacaat
     8581 gaaagagtta ttgaaagtgg ctaaagagaa tcaatttgca gtaccagcct tcaatatttg
     8641 cagctatgat atgatgaaag caatcatcga agaagcagag agattagaag caccagtaat
     8701 cttagaaatt catcctgatg aaatcgccta tttaagtgat gagtttgttg cggcagtaag
     8761 agcctatgct tatacaagta aactgccgat cgttatccac ttggatcatg gcggtacgat
     8821 agaagatgtc ttacgggcga ttcgaaacgg ctattcttcc gtgatgatcg atgcgtcttt
     8881 gaaatcattc gacgacaatg tacagattac gaaaaaagtg gttgcgttag ctcatgcggt
     8941 aaatgtctca gtggaggctg agcttggaac gattgggaat aatggttctg ctgaaggagg
     9001 tgctgaaaca atcatttata cagatcctga tcaagcgaag atttttgtga aagaaacagg
     9061 aatcgataca cttgctgtag caattggaac agcgcatggc ttgtatccga aaaacagaac
     9121 tccaaaatta aatatcgact tactgaaaga attgaaccaa caaatcgatc ttccatttgt
     9181 tttacatggc ggttcaggta atccggacgc agaagtcagc gaagcagtaa aatacggagt
     9241 tgccaaagtg aatttgagct cagatcttaa aagcgtcttt ttcaatgctt tgagagaaat
     9301 attgagtgaa aatccatcga tgtacgagcc gaatgcaatt tatccagcag ctaataaaaa
     9361 ggtacaagaa gtggtcaaac ataaaatgga aattttgaac acagtaggca aggctagttt
     9421 atactaaaaa taaaaatgtg tggtcaagag ggtatgacaa tagtcttgtc ataccctctt
     9481 aaccacacac gatgttcaga agtagttttt tcgaaaaatt tcttcttatt tctcgaagcc
     9541 aatcacttct gtcacaacct cttttttcct gttactatat aaagtaaatg gggtgatttt
     9601 atgttgaatg aagtggagaa tagacagcag gagattgtga attatctaaa aaacaaccaa
     9661 tttgcaagag tcaccgattt gatcgagtta gtaaattata gcgaagccac gattaaacgt
     9721 gaccttgttt ttctggaaaa aagtggattg atccgtagag ttcgcggtgg cgccatgctt
     9781 attgacaatc gaaaaattga cgtgccttac ctgatgaaga tcaccaaatt agaagaagaa
     9841 acagaaaaac gtaagattgc agatacagct tcttctttga ttcgagatga tatggtctta
     9901 ttcttggatt ccagtacaac ctctttacac ttagtcagaa acctagcaag atttgatggt
     9961 ttgcagatca ttacgaatgg catcgtcaca gctgctatgt tgagtgaata tacaacggca
    10021 aaagtatctg tattgggagg ggcaattgtt ccgaaacgag caacaatcaa cggttcaaaa
    10081 gcctataacg atgcattgtc atattatgcg gatttagcga taatgagttg tcgcgggcta
    10141 gatttcagcc aaggagtaac agaaactcat gaaggagagg caatggtcaa aaaagctttt
    10201 agaaaacaat cagaccggct tgccgtttta gttacacagc aaaaaattgc acataaattc
    10261 atgcatcaag ccattgcttt tcatgaaatc gattatctgg tgacaagtaa agaactgaca
    10321 aaagaacagt atgcgaaagt aaaggaacac catatcgaat gtctgtacgg ataaattatt
    10381 acgtgaatgt tctaagtggc ttcctcattt ttagatagga ccttatcttt gctcgcttct
    10441 ctcagccgat ggtataataa tgaaaaggtt atcttgttag ataatcttac ttttttatcc
    10501 aggagaaagg agtgcagaaa atgaaacatg tgaaggcgtt ggtagtgaag gcaatcatga
    10561 tctgggcgat tctgtgggtc gtgctgacag gtttatacgg tgttagcttt atggattcaa
    10621 cgattgttgg ggtaattatt gtagtcatga tctatgtgct aggggatctg ttgatcttac
    10681 gtaaagtagg gaacatcgca gcgacgatcg cagatgctgg ttcagctgca gttatcttgt
    10741 ggctctatct atattttatg aatgatacag tcgatatttg gatgaaagta ttgattcctg
    10801 ctctattgat cggtgtggca gaatggttct tccacaagtg gttgctaagt caaggcatcg
    10861 taccagatga acggaaaatg gaataaatca acagttaaac tattcttatc gataaatgaa
    10921 agctttaaaa ataaatagat gtttaattaa gaataatcat aaactatttt tactcaaaaa
    10981 accgctcaga ttattttcag atctgaacgg ttttttttat ctattcatca agatcaaatt
    11041 tcatttttca atgattcaat caattcgccc tctgcttcat gactatgttt tcggccatta
    11101 acaagataga gtgctgtaca tatcaatgga atgactccgg caatcacata caatcctcta
    11161 aatgaagtaa gagattttag catccccatc aaataaggac caacgcctaa gcctaggtct
    11221 aaaccaataa agtaggtaga taaagcaata ctgattcgat gttcgcggac gatttttaaa
    11281 caaacagcct gtccatttga cataaacgta ccataaccta ggccgactaa tgctccagag
    11341 ataagcaatg tcacactatt cgtcgttaca ctcagtaaaa ataatcctgc tgttaagaat
    11401 aaatagctag gatacatcac gtattgttct cctttgacat cgaaaatcct tccagtcaat
    11461 ggacgtgtaa atgtgatgac caaagcatat acgacgaaga aaaatgaccc agcagcaact
    11521 aagtggatta cttttgtata agaagataaa aatgataaaa cacttgaata agaaagaccc
    11581 atcaaaaagg caattcctgt aatgaaaagt gctttctttt caataaaact attgatattc
    11641 catgagcgaa gagatatttt ttgttcttca gataatggaa tattttttac aggaaagaca
    11701 aggcaggcaa tcgtaacaaa gaagaccagt acaattgaaa accagataat gaaacgaaaa
    11761 tcagtcatat tcagcaaaat cattcctaaa aatggcccaa cagcggcagc taaactagta
    11821 cttaatccat aataattgat gccttcccct tttttagatg cagggatata tgcagtaacg
    11881 atagcatttg tagctgtaga aactgtaccg tagccgaatc cattcaagaa tcgaactaaa
    11941 tacaatgccc cgatagttgg cgtataaaga tatgctaatg ttgaaaggag atagaagatc
    12001 gcgccgcctc ttaaggtaaa cttccgacca aatagttcta actgtttccc catgaataag
    12061 cgtgccagca atgttccgat gatatagatc ccagaagcaa aacctgcttg gctcaagctg
    12121 gcgtgtaagg tgtcttgcgc aatgactgcc atgatgacca tcaacagata ataaatcagg
    12181 tagacaataa aattgatcaa tgtgattcca ataaaatatt tattaaataa tttcttttcc
    12241 atgtatgtta tacattcccc ttaagctgaa tatgctttta atttttgtat ttgaacttct
    12301 acatcgtgat aattggatgg atagcgatca acaagttggc gaataaataa atctcttgta
    12361 gacataaaca gatttaaaaa ttcttcgcat tcgtttgttg gattctcatc cactgctttg
    12421 atcaattcga tcgagttttg atacgcatca tttaaaaaga ctaaagaagc tttcatatca
    12481 aatcttgttt gatatgctgt ttctaatcga ttaatgattg tgttcatcca gtaattcatg
    12541 ttttttccct ctattcttaa aaaattttac ttctgcagta taacgaataa aaaaaaggaa
    12601 aaaaatcgca taagtgatgg aaaaaggtac ttaacaaatg ttaatatcat tagtaaaata
    12661 cctttggact ttaaaaagtt ttaaagtact gtatgatctt tttagatgaa agtttggatg
    12721 ggggagcatc attggatagt agagtcttaa aggaatattt ggataaaaat gattttccga
    12781 ttatacgcaa aggatatcat aaatatttga cattcgaagg gatggaagat tcccatacgt
    12841 atatcttgaa agagggaacg gtaaaagcaa gtgttatttc aaatgatggg agagaattta
    12901 accttcgcta tatcaaagat cttgaaatcg tctctctttt aaaagacgaa tattcacaat
    12961 tcatcgattc gccgtataat attcgagttg aatcagaata tgcagagttt tatcgaatca
    13021 atcgtgtgcg cttttggcaa aatatcaacc aagacccaaa gctacaacac tatgtaaaag
    13081 agtattaccg ctatcgtctg ctttattcga tgaaaaaaat gcagcaaatg ctattaaacg
    13141 gaaagctggg agctgtttgt acgcaacttt acgaattata tgatttattc ggtgtgcgga
    13201 cagaagaagg gatgctgatc gatttttcga tcacgaatga agaaatcgcc aaattttgtg
    13261 gcatctcaac tgctagcagt gtaaacagaa ttttaaaaca actgaaaaaa gatggtatta
    13321 tcaccgttaa agaccatcat atcgttattt tgaatatgga cttgttagaa gacaatatta
    13381 tcttttgatg aacaaagagg ttgtgaaatc agcgttttga cctgagtatt agagaaacaa
    13441 acggaaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagctgat
    13501 tgaacaccgt ttaccaagag gttgtgagcc tgccgtttaa ctctgagtca caaggaacaa
    13561 tttcaaaaaa cagcttttca tgtttgatga atattgtgac ttgtgaccga ggagttggta
    13621 ggagaacacc gtttaccaag aggttgtgaa aaagctgttc tgcatcaagt aataagaaga
    13681 gccaagtaaa aacagtctct tatgttttta acttggctgg acttatcacc gtagatgtaa
    13741 gcttttgaac gccgtttatt tgcaacaaaa ggggctgtga cacgattttt gtcacagccc
    13801 tttttttgcg aacattcatt tagcaatttt tagaaacttc tgccgccacc gccaaaagtc
    13861 ccgccaccgc tagaatgggt agtacttccg ccaccgccgc ttccaggagg tgggcttttg
    13921 gggattcttc ttgtcgtgac aaaagaattt gtcagacgat cggttttttc agttaatttg
    13981 atactggatt tttctcggaa tggatatttg tagatgcctg atttcaattg ataacgcgaa
    14041 acagtcacaa aataaaagac aagactaagg ataagagcca aaacaacagc aatgatgatt
    14101 tctactgtcg tcaaaacctt atagcgtgta attttgccag tttcttcgtc tacacgataa
    14161 tgtcctcctg gaactccgtc ttccacatat gttgctgatt tagacaggta agcttttgca
    14221 gcattgtaat aggcagcatt tgtcatctga tcataaacat catctaagat cgaatcgatt
    14281 ctgctatcag tcaaataatc gatcatatta ccagaagtcg aaatgtagat ttcacgctgt
    14341 cccatatcta atagcaatac tgagccattt tgatcatttc cgactgcgtc tcttaagtaa
    14401 ttatcagcaa agtctcttgg ttcttctgaa ttagatgtag tcgttacgat caagactcgt
    14461 cctttgattt tttcattcag cttttctgcc tgttgttcca aagattggat ttgatctgtt
    14521 gtgaataacc cagcctcatc atttaccgta gctgtatctg catgagtagc caccggccag
    14581 ccaacactta ctagcaaaag aaagaataaa aaaaattttt tcatcatatc aagtaacctc
    14641 cgatcagaaa gaggatcgct agcccagcga aaatcccaag agcggtcagt cccaattttt
    14701 tctggctgat cggcaggaca ccgctgacct ttcctgtttg tccattcatt gcgtagtagt
    14761 agactttttt acttgagtca ttgcttcgat aagtaacaag ccataccggc agtaatacgt
    14821 aattatttac ttcgtttgtg attgaagcag aagttcgcac accggtcaat gttgtatatc
    14881 catttgcggt atcttttaac agcttctctg aatagtcttt taattcagat tgaatctgtg
    14941 atttgattgc ctcatattcg atatctcttt tttctgcttg gaacccggct aaatactggc
    15001 ttttaaaatc aattgcttta tctaacggga aaggttgcac gccttcgacc atcttttgtt
    15061 gcgtattttt tgataaggca ttttttacta gttctttaaa agaaagcttg cctgctcttg
    15121 aaacagcaaa ttgcttcgtt tctgtgtatt cgatgtcacc tatccgccat atgcggataa
    15181 ctgtaccatt cgcctgcatg gcaccatcta cttcagcatc tgtagcccaa taagggaaat
    15241 acacacctgt caacttatcg atttgatcct tattgaaaaa agctttggga acaaaccatt
    15301 ttttcttggt ccaagcgaga aatctgttga ttgcttcttc tttctcgaca gcaaaaggaa
    15361 gtactttatt tggcaagaat tctccgctta aacgtcccga tagtacgacg ggattatggc
    15421 agtagtaaca ataagtcgca gcagttgtcg catcagtcac gatctctgca ccacagttgg
    15481 gacaaagaaa aagatctatc gctgcggtat tatctgcaga ttcgtccgct gtatctgaaa
    15541 aaccgcctgc ctcgtcaacc gctttgcgct cggcttcatt catatcggca agctgttctt
    15601 gggctgtaaa agtaaaatct tcggtaggtg aaggttcttc tttgctttct tcgccagcca
    15661 tacgcgcttc tttttgcttt tgctcatatt ggctgacttc ttcttctgta tagatattca
    15721 ggcagtattc gcaatgaaat ttttggtctt tgggatcaaa agtcaacgga ccgccgcaat
    15781 taggacattt atgtgtaaga acatccatca gctgcacacc tttctaaata aattaattaa
    15841 cggggattcc tttaaacggt ttaccacagt ttggacagaa tttagggatg ccgttggaaa
    15901 gatccacgat ctcattgcat tcactgcatc gcatttgtag cttaggctga acgttttcta
    15961 caggtttggc agctccgcag ttggaacaga attttccgga attttctgtc ccgcaaactg
    16021 gacatgtcca agtatccgca tttccttgtg cagtttgttg attttgtttt tctgcttgtt
    16081 gtttgatttg ttcttggtta ttttgagctg cttgagaaag ataagaacca tttgcattca
    16141 ttcccatacc tacacccata aagccggtca ttgctcctgc ttcatttttt ccggctgctt
    16201 ccataccacg tgcaatggaa ccttgcacgt atccttcacg tacgctagga tcacctagca
    16261 tcgccccttc gttacgcata ttgatcagtt tcacagaatc atcggtgtaa gaaatgctgg
    16321 ctacagccac agaaacaatc tccatcccac gtaattcacg ccaagaatca tccaatgcag
    16381 tatccatgta tttgcttaat tccaaactct tagatggtac ataagaaatc cgttgaccat
    16441 ctgcagacat ctgattgatt gctgattgta gcgctgttag gaattcagct aaatactgct
    16501 cattgatatc attgatttcc acttgtgttt tgtttttagg gattgcgttt gtataaaata
    16561 aaatcgggtc aacgacatga atagaataag tgccatgtgc ccgtaagaat aattctgcgt
    16621 tatagaaatt gtcaaagtag ttcaacggcg cgcttgtacc gaatttgatt ccctttattt
    16681 cttggagatt gatataaaaa acttgttgtt tctgcggtgt gacgccgcca aatttgaatc
    16741 ggtcaaaagt ttcagcaatt gcttctttca gtgttccatt gaacatggaa ggagctgcgt
    16801 catttttaat ggtgtaataa ccttcctcag cggtataatc gatgattttt ccaccatcga
    16861 ccaaaagcat catcatgttt ggatagacat ggacgactgt tccgtctgtt aaaacatctt
    16921 ctgttccttt acgattggag ccacgtttgt catctttgcg gactttgaca ccttttgtca
    16981 tgaccgttgt atcactcata ttgtctggtt cgatgacttc tagccattga tctgccagac
    17041 cgccgccaac catacttgtt gctgctttga taagacccat aaaaaattcc tcctctgatt
    17101 cagctagtgc caaacaatta ttgtttattt attataccat aggaagatgt gaaacttctc
    17161 ggactagaga cggaattgaa atggaaaatt gcgtattgtt taaatacctg aaaaagccga
    17221 gaaggatagt agttttttta cctaatatca cgtataataa gagatagtga attttttaaa
    17281 aaagggggat ccttatggca tatcaagcac tttatcgagt ctggcgttcg cagcgtttcg
    17341 aagacatcgt tggacaaaaa gctgtgacac agacactaaa aaatgctatt gtttcccaca
    17401 aaacctcaca tgcctacttg tttacaggac ccagaggaac cggaaagacg agtgcagcaa
    17461 agattttcgc aaaagcaatc aactgtccga acagtaaaga cggtgagcca tgcaacgaat
    17521 gcgaaatgtg tcgatcaatt actgctggta cgcaagaaga tgtgatcgaa atcgatgctg
    17581 ccagcaataa cggagtcgaa gaaatccgat ttatccgtga tcgggcaaat tatgcgccta
    17641 caaaggcagc gtacaaagtg tacattattg atgaggtaca tatgctttca acaggagctt
    17701 tcaatgcctt gttgaaaaca ttggaagagc caaaagaaag tgtcatattc atcttagcaa
    17761 cgaccgaacc tcacaaaata ccagctacga tcatttcacg gactcagcga tttgatttta
    17821 aacggatcaa tacggcagat attgtcgaac atcttgaatt tatcttgaaa gaatcgacta
    17881 tcagctatga accagctgca ttgaatgtga ttgcccgttc tgcagaaggc gggatgcggg
    17941 atgcattgag tattcttgat caggcaatct cgtttagtga aggaacaatt actttagatg
    18001 atgccatgca agtgacaggt agcttaactg acgaaatgat ggacagttac ttgagtgctt
    18061 gcgtgaaaaa agaagtacca gaagcattgg aaacattgaa ccaaattctc gcagccggca
    18121 aagaatcacg tcgctttcta gaggatatgt tacaatactg ccgtgacttg ctgatgtatc
    18181 agcaagcacc taacctagtt gaggaacaat cgagtacgat cagaagtaca tttacagaat
    18241 tggcacaaac tgtttcagcg gatcagctgt tttcaatgat aaagattttg agcgatactc
    18301 aaaatgaaat tcgttttacc aatagtgccg ctatttattt ggaagtcgct acagtcaaac
    18361 ttgcacagct accgtctgct tctgttcctt cagcagatgt agcatttgca gatgatcaga
    18421 tagtaaaaca gctacaacaa gagattaagg aactaagaaa cgaaatcaag gaactacgca
    18481 gtaatacagg aattgagaat acaaaagtac agccggtaca gcaaacaaat cataaaaaac
    18541 agacaagtac ctatcgtgta ccgacggaac gagtatataa agttctgcgg gaagcgacaa
    18601 gacagcatct gaaccaagta aaagaagtat gggaagactt gctgatgagt ctatctgtca
    18661 ctcagcgagc aatgcttaaa gcaagcgaac cagttgcagc tggacctaca ggactagtga
    18721 ttgcgtttga ttatgagatt gtttgtcaaa gagcagcaaa tgacgaagaa ttgcagcttt
    18781 cggtacacaa caacttaagc cgaatgatca aagattatgc gcctgatcct gtttatatta
    18841 caagagaaag ctggccagaa cttcgaagaa attatttgac gcaggcacaa gaagagggaa
    18901 cagaaaactc ttttgcttct gaagactcag aaggagaaat cgagttgctt cctcaagaac
    18961 aagaagtacc agaagaaaca cttgtagtaa aagcagagga actgtttggc tccgaagctg
    19021 ttacagtggt agatgattaa taaaaaggag tagataatta tgatgcgtgg aatgggcaat
    19081 atgcaaggca tgatgaaaca agtacaaaaa atgcaaaaag aaatggcaga agcacaagag
    19141 ctgctaaata aaacagagtt tacaggtaaa gccaccaatg aattggtcaa agcggttttt
    19201 acaggcgatc gtcgcatgaa agatatccaa atcaatgaag aagtcgttga tcctgatgat
    19261 atcgagatgc ttcaggatct tgtcgtaatg gcagtcaatg atgcgatcgc acaaattgat
    19321 gtggaaactg agaaaaaatt agggaaatac gcaaaaggtt tgccaggatt ttagttttaa
    19381 actgaaggag taatgaatat gcaatatcca gaaccaattg cgaaactgat cgacagctac
    19441 atgaaacttc caggaatcgg tcagaaaaca gcgacaagac tcgcctttta tacgatcgat
    19501 atgaaagatg aagtagtaaa tgaatttgct aaatctttgt taagtgtaaa acgcgatttg
    19561 catttttgta gtatctgcgg aaacatcaca gaagaagatc cctgcgaaat atgtagaaat
    19621 ccttcacgtg atcgaagcgt tatcctagtg gtcgaagagc cgaaagatgt aatggcaatg
    19681 gaaaaaatgc gagaatatca cggactttat catgttctcc atggtgtgtt atcaccgatg
    19741 gaagggacag gtccagaaga catcaatatt tcttctctgt tgcagcggct gcatgatgca
    19801 gatgtaaaag aagtgattat cgcaacaaat gcaacaacag agggagaagc aacagctatg
    19861 tatctctcac gtctgatcaa acccgcaggt atcaaagtga ctcgtctagc acatggtctg
    19921 tctgtaggct ctgatatcga gtatgcagat gaagttactt tattgaaagc tgtcgaagga
    19981 agacgtgagt tataaatttc taattaagtg ttttttcaga tacaatataa aagcagcaca
    20041 agagatgggg gcaacaatgt gaacggacta tttattacaa tcgaggggcc tgatggtgca
    20101 ggaaaaacca gcatcttgaa tgaactttac cctcttttga aagaagtagc aaaaacagaa
    20161 atcattcaaa caagagaacc aggaggcatt ccgattgctg aagagatccg agcagtgatc
    20221 cttgatccaa aaaatgatcg gatggatgaa cgtacggaag cactgcttta tgcagcagca
    20281 agaagacaac atctagtcga aaaggttctg cctgctctgg caaaagggaa aatcgtttta
    20341 tgcgatagat ttgtcgacag ttctcttgct tatcaaggtg caggccgacg catcggcgtt
    20401 accgagattg ctcggttgaa tgagtttgct acagaaggta ctacgccgga ttttaccctt
    20461 tatcttgacg tagattcaga caccggatta agaagaatca agaaaaacag acaaaatcaa
    20521 atcgaccgtt tagattcgga aggtttggaa ttccaccagc gtgtacgtca tgcttactta
    20581 aagctggcag aagagaatcc ggaaagaatc cacaaagttg atgcaagaaa aagctttgaa
    20641 gaagtgctgc aaacaagtta tcatacaatt attgaacagt atccgcaatt ttttgaaaac
    20701 taggaaggtg aacgtttatg aaaattattt tagcgattat ccaagacaaa gacagcaacc
    20761 gtttatcaaa tgaattgatt gatgcaaata tccgtgccac aaaactatca tcgacaggcg
    20821 gcttccttaa ggctggaaac agtacgttta tcgtgggtat tgaagatgac cgtgtagaag
    20881 aagcattgga aatcatcaaa aaaacgtgtg aatcaagaaa gcaatttgta tctacacctg
    20941 ttacactgga tatttcgatg gatgggggcg tcccttatcc agtagaagta gaagtaggcg
    21001 gtgccacagt cttcgtctta cctgttgaag gattccatca attttaggag gagctgagct
    21061 atgaatcaag aaataaactt ggctcaaatg caaccgatcg tgtatcagca gcttcaacgg
    21121 agttttgagc atgggcgtat cgctcatgct tatctttttg aaggggagaa aggaacagga
    21181 aaacatgaag taggtatatg gcttgcacag cacctgttct gtacccaaat gaaagacaat
    21241 ctgccttgtg gaaaatgcaa caattgtcag cggatcgaaa agaaagaaca tccagatgtt
    21301 ttggttattg aaccggaagg acaaaccatc aaagtcgatc agatcagacg tttgcagacg
    21361 gaattcagtc gcagcggtta cgaatcaaga aaaaaagttt ttttgatcaa agaagctgaa
    21421 aaaatgaata gcagtgcggc aaacagtttg ttgaaattct tggaagagcc ccctggagat
    21481 tttttagcgg tgttagaaac agatgcagta ggaagaattc tccctactat tcaatctcgt
    21541 tgtcaagttc tgcattttca agaactttcg aaagaagcac tgatccatcg attgcaagaa
    21601 gagcagattc cattggaaaa agcaaaactt ttggctttct taacaaacag tttaggaaaa
    21661 gcagttgaaa tctctcaaga tgaatggttt aatgatgcta aggacttgat ttatcaatgg
    21721 ttcgtttact tgcaaaagca agatactcaa gcctttatct atgtccaaaa aaaattggta
    21781 aaatcattta aagaaaaaac acaacagttt acaggattat ctgtcttgat gttttattat
    21841 caggaagcac agcgtcaagc agtagcaaac ggcgaatatg gcaaagtgaa acagataaac
    21901 cagataattg aacggatatt gatcgcagag cagaaactac gcagcaatgt cagttttcag
    21961 gctgttgcgg aacagttcgt tttgcatacg attttttagc ttgccgcaaa ggatgatgat
    22021 caaggaggat ggaaatggta gaagtagtag gtgtccgata tagagatgca ggtcatatct
    22081 attattttgc accaggagac tctgaatatg tttatagtga caaggtatta gtcgaatctc
    22141 agcaatcctt acatattgct actgttgtcc ttcctaaaaa agagattgat cccaatgacc
    22201 ttcctgaagc tatccgtccg atcttgcata aagcgtcaca agctgaatta gaaaaagaca
    22261 aaaaaaacaa aaaagatgct tatgatgcat tgaaaatcgc aaaagaaaaa gtgcagttgc
    22321 gtgagttgga aatgaaaatg gtcaaagccg aatacacatt tgatcgaaat aaattgattt
    22381 tttctttcac agcggacggg cgaattgact ttagagaatt ggtaaaagat ctggccgctg
    22441 tatttcgtac tcgtattgag cttcgacaaa ttggtgtccg cgatgaagca aagataatag
    22501 gcggaattgg tccatgtggg cgtccattat gctgttcttc ttttttaggc gatttcgtgc
    22561 ctgtatcaat caaaatggca aaagatcaaa gtttatcttt gaatccaaca aaaatttccg
    22621 gtttatgcgg acgcttgatg tgctgtctgc aatatgagag cgaagaatac gagacagcga
    22681 aaaaagaact ccctgacttt ggaaaagtaa ttgctacacc tgatggtaaa gggaaagtcg
    22741 tagggttgaa tctgcttagc cgtatcgtta gtattcggct gactggacgg gaaaatcctg
    22801 tcgaatatga atgggatgaa ttgaaagaat ttatccaagt aggtgagatg aatggataag
    22861 agatcattat acgacggatt gaatcaatta gaatcagagt tgagaaatac attaacagaa
    22921 cttgttgaaa tgaagcatgc tttgcatgag atcgtagaaa aaaatacaac tttggagatg
    22981 gaaaatcaac gtctgcggga acacctgcgg gaattgaacc aagttgcaca agctccagct
    23041 gaaaatgtga agcaggaact ttctaagtct cgtatgaatc ttgaaaaaat ttatgaagaa
    23101 ggattccatg tctgctacga tttatatgga tcaagaaggg acaatgacga gccttgtgcg
    23161 ttttgtctag aagtgatcta tcgcgaaagc ggtcgatgac caataacaag aggagactgg
    23221 agcggagaaa tactgctcca gtctttttat taaggagatt gtgacagatg tataatcaaa
    23281 aaagtttcaa gggaaatccg gtatatgggg atttatatct tgtaccgacg ccaattggaa
    23341 atttagaaga tatgagctac agaagtgttc gtatgctgca agaatccgat cttatcgcaa
    23401 gtgaggatac gcgtaataca caaaagttat tgaatcattt tgaaatccat acaccccaaa
    23461 aaagtttaca tgaacataat tataaagaac gtgtccctca attgattgct ttgttaaaag
    23521 aaggaaaaac gattgctcaa gtcagtgacg caggaatgcc ttccatcagc gatcctggac
    23581 atgagctagt tttagcctgc ataaaagaag ggatttctgt gatctctatc ccaggaccta
    23641 cagctgggat gacggctttg attgcttcag ggctagtgcc tcaaccattt ttattttatg
    23701 gcttccttcc acgtaagaaa aaagaacaga aagaaacatt gaaaaagcta aaagaacaga
    23761 cagttacgct gattttttat gagtcacctt atcggatcac tgctacattg acgaatttct
    23821 tagaaatttt tggaaatcgc caagtcgttt tgtgtcggga attgacaaag gtccatgaag
    23881 aataccttag aggaagtacg gaagagctac tcgcttatat cgaagaacat ccagtaaagg
    23941 gtgaatgttg tctgcttgta gaagggaatt tgttcgaaat gccagtgacg gaagttgatg
    24001 aagagttggg aacgttaaag gaacaagtag aagcaaagat cgcttcaggc gaaaaaccaa
    24061 atgctgctat caaagcagta gcgctgaaaa atgggctgaa aaaacaagaa gtctaccgtc
    24121 agtaccatga attagactga gatagtataa gttgtctata atgaaagact aatgaggaaa
    24181 tcagctacta acttttcaaa tctcttgaac gcgaacgaat gttcttgtat aatgaataag
    24241 aggtgaggat gatggagctg caatttcctt tacataatga tacatcaaga aaaatcattc
    24301 atattgatat ggatgctttt ttcgcttcgg ttgaagaacg agatcatcct gagtttcgcg
    24361 gacatccatt agtaattgcc agacacccca gtgacactgg tgggcgtgga gtagtgacta
    24421 cagcaaatta tgaagcaaga aaattcggaa ttcattcagc aatgagtgca caaaaagcgt
    24481 atgagctttg tccgcaagcc atctttaagc ctggcgacca tcataaatac agcgatattt
    24541 ccaaacaagt acgggaagta ttctatcgtt atacagatat tgtggaacca gttagtatcg
    24601 atgaagcgta tcttgatgtg accgaaaata aaatcaattg caagtcagcc atcaaaatcg
    24661 cccgaatgat ccaagcagat atttgggaga gtgtccagct aacatgttct gcgggagtaa
    24721 gttacaataa gtttttggcc aaattagcat cagactatca aaaacctaga ggaatcacgg
    24781 tagttgcccc tcaagacgct gtgccatttt taaaagcttt gccaattgaa aaatttcatg
    24841 gggtagggaa gaaaactgta cctagaatgc atgagctagg gatttataca ggaaaagacc
    24901 tatatgaatg cacagaaatg atgttgattc gaaattttgg aaaaatgggc tattctcttt
    24961 atcgtaaagt cagaggaatc catgattctc cagtaaacgt tacaagagag cgaaaatctg
    25021 tgggaaaaga gcatacttat gggcagccgt tgcaaacgga agaagcagtg ctgactcagt
    25081 tgcgccaact agctgaaaaa gtagaagaag cactgagaag agtccaaaaa catgggaaaa
    25141 cagtagtttt gaaggtcagg tacacggact attctactgt gaccaaaaga gtgacattgc
    25201 cagaatatat ctataaaaaa gaagcactct tttatcaagc aagccttata tgggaggaaa
    25261 tcttgggagt agaaaaagga atccgattgt tagggattac tttgacgaat ctggacccga
    25321 tgacttatga aaacatcgtc cttccattat gggaaaatca agaaatataa aaacagcttt
    25381 gagagttgaa accggaaatc cgttttctct cccaaagctg tttttatatc aagtcttgct
    25441 ttttgatttg ttcataggtt tctgttgcat ccgtacattt ttcccaaatc acaacttgat
    25501 tggattctaa agatttttgg acgtcaatga tccgctggtt gctacttccg cgaaattgta
    25561 gttttaggtc acgtttgctt aattcaaagc gtccgtcaac aaggatatcg atttgcgaaa
    25621 ggagttctaa tttatcttct gaatcttgta gcaattcatc aaaggtatag cctgtccatg
    25681 accagatatc ttttgtcgta ccgaactctt ttcggatacg tcgtacgact tgcagacaaa
    25741 catctgtatt caaaaaaggt tcgccaccaa gtaaagttaa tccttgcacg taatcgttgc
    25801 gcaagtcagc gatgatttgt tcttctaatt caggtgtgaa tggtttgcca tatcggaaat
    25861 tttgtgcagc tttgttgaag cagccttcgc aggcaaataa gcagccacta acgtaaagac
    25921 tgttgcggac accttcacca tcgacaaaat taaacggttt gtagtcagca atatagtttt
    25981 ggctgtagtc ttttgctaac cattcttttg gttttggatt tctcataaaa aaacttcttt
    26041 catttaaggt tcaaagatat agctattgaa gataaaatct actcgaccac ataaataaga
    26101 acaaactgac ctaaatccat tgttcaagaa agcttgttct tattttgata gagggcgttc
    26161 aaagatacaa agccatcgaa agagggtggt gaaaaaagac ttgtcatagc ctcttattct
    26221 gaataaacgg tgttcaaaag cttgcatctg cagtaataag cccaactaaa cgaaaaatat
    26281 gaggaactat ttttgtttag ctattcttat tacttgatgc aggacagctt tttcacaacc
    26341 tcatttcatg tgtttcacac gtgaggaaat ttctttatgg cggccatgta ccatcggacg
    26401 agcttgcgga tttcctaaat agccacaagt acgtttgact acatcacatg tttttggatc
    26461 atgatttcca cattctggac attcgaaccc tcgttctgtc ggtttgaaat caccttcgaa
    26521 accacattga tagcaatgat cgattggcgt attcgtacca agatacccga ttttgtcata
    26581 tgcaaaatcc catacagctt ctaaagcttt cggattttgt tggagcatag ggtattcaca
    26641 gtagtggatg aatccacctg aacaatattt tggataatcc ttctcaaatt ccaatttttc
    26701 aaatggcgtt gggtttttac gcacatcata gtggaaactg tttgtataat attctttatc
    26761 tgtgatattt tcgatgatgc cgaatttttc tgtatccaaa cggcagaaac ggtcagttag
    26821 actttcactt ggagttgaat agacactgaa atgataacca tattcatccc cccatgcatc
    26881 tgcattagct tttaattctt tgacgatatc aagtgtgaaa gcttttgctt ctgggtttgt
    26941 ttcccattcg ccaccgaaga aagctgaagc aacttcatac aatccaatgt agcctaacga
    27001 tacagtagct cgacggtttt tgaataattc attgacagaa tcggtacgtg aaagacgttt
    27061 cccgaaagca ccatacatat ataaaattgg cgcatttgct ggaatggctt ctttacaacg
    27121 ttctacacga tagagaagcg catctttcat gatttctaag cggtcagaca gtaactgcca
    27181 gaatttattc tgatctcctt ttgattcgat cgcgattctt ggcaaattca atgtaacaac
    27241 acctaaattc attcgtccga catttacttc ttgaccattt tcgtctttcc acccttgcaa
    27301 gaatgaacga cagcccatag gtactttgaa acttccagtt aattcaacga ttttatcgta
    27361 attcagaata tcaggataca ttcttttcgt tgcacattcc aatgctagtt gtttgatgtc
    27421 gtaattagga tcttcagggt ttaagttcac accttttttc aaagtgaaga tcagtttagg
    27481 gaagatagct gtccgctttt cactccctaa tccattgatt cgaatctgta aaattgcttt
    27541 ttgaatttct ctttcaaacc agttttctcc tagtccaaat cctaatgaag tgaaaggagt
    27601 ttgtccattt gaagtaaata aggtattgat ttcatattcc aaactttgca tagcatcgaa
    27661 gatatctttt ttggtttttg cttttgcata ttctttttgg cgttctggcg aatcgatcca
    27721 ttcttccgcg tcttttagat gttttttata gttcaattct gcaaaaggag caagcaattc
    27781 atctgttcgg tcggcagaac agccaccata ttgacttgac gctacattgg caataatctg
    27841 tgagatttgt gctgtggcgg tctggataga ttttggcgat tcgacttcgg cattgccgat
    27901 tttgaaccca ttgttcagca tgcctttgaa atcaatcaag cagcaattcg tcatcggtgt
    27961 ataaggatga taatccaaat catggtaatg gatatcgcct ttttgatgcg catttgctat
    28021 atgtggtggc aacattttca gtccgattga ttttccaacg atcccggctg tcagatctcg
    28081 ctgtgtattg aaaacattac tgtctttatt tgcgttttcg ttcacgacag tttggtcctt
    28141 gttgattagc ttttcaatcg aaaagttgat atctgttgct ttactgcgag caaaatctcg
    28201 tttggtacga taattgatgt actcttctgc taattcatat tcgttttttt ctagtaatat
    28261 atgttccacg atgttttgaa tctcatagat tttgacattt tcagcaaacc ggcttttgat
    28321 ttcttggaca accaagtctg caatcatttg aattttttca taagtaagtg gtgataatga
    28381 gccgtgaatt ttcttttctg ctttgatcag tgcttcattg atcttttgtt catcgaaatc
    28441 aacatggcgg ccatcgcgtt tgatgacttt catctgggat gtctctggct gctgagtaac
    28501 gacagttttt tcttcataat ttactttcat tatcttcaac tcctgctaaa ttgttcttgt
    28561 ctatcataaa acaaaatcgg ggaaggagca actaaatatc ggctatatat agtattcaat
    28621 gtgctgtata attggatttc tatactatat gtagtgtata ttttattgtg aaaaaaggtg
    28681 aaaacttgta gctggtggat ttgttttttg catatttaat tttgctgaat aaaataattg
    28741 aataaaattt tttattcagt ccaagtaaaa gtgacaaaaa cataagagca aattattcgt
    28801 agatatgcta ctatgcaaat aaatagaaat gaaaaatgaa agaggaggag aagaattgga
    28861 aaagatgatc gaagtgaaac atctaagtaa aagctacggc agcaaacttt ctcccgtcaa
    28921 agccttagat aatatctcat tcacagtaga agaaggcgag tttgttggga ttatgggccc
    28981 aagtggtgca ggaaagacaa cgctgatgaa tatcttgtcc acgatcaatt tgcctacgat
    29041 gggcagtgtc tcgatccaag gaaaagaaat cacaaaaatg aaaaacagtg agctcagtga
    29101 ctttcgtcga aaaaaactag gattcatttt tcaagagttc aatttgatcg ataccctaaa
    29161 tgcaaaagac aatatcctat tgcccctagc agttgaacgg atgactaaag aggaaatgga
    29221 aaagagagta agacatgtag ctcaattgtt gaatatagaa gaactgctca aacggtatcc
    29281 cgatgaactt tctgtcggac agagacaacg aattgcagct gctagagcac ttgttgttca
    29341 gccacaagtg atttttgcag atgaaccaac tggctctttg gactcgaagt cggctacaga
    29401 attgttgaac tacttgaaga ccatgaatca aaaagaaaaa gcaacgatac tgttagtcac
    29461 ccatgatcca tatacagcaa gctactgcga tagaatttta tttattaaag atggtgtgat
    29521 tttttctgaa gttgtcagaa gagggacaag aagagagttc tttgagaaag taatagacat
    29581 gcaggcgacg attggcggag gaggaaaaat gaatgctgta taaactagcc gttaaaagtt
    29641 tttttcggca gtcacgaggc tatcttgttt attttttcag tttgacttta tccgtaatga
    29701 tctattattc cttcagcgca atgacctacg atcaatcttt agtcagaaga gcaagtcaag
    29761 atacttcgat agatggtgga ttgagttttg gtggatttat cattactgtt gttttgcttt
    29821 ttttcgtatt cagtgccaac cgatttttct tgaatagaag gcaaaaggaa ataggcatct
    29881 atcagctttt tggtatgaat aaactgcaga tatcaggtat atatgtgtta gaaatcatga
    29941 taattggatt gttcgcctgt atttctggga tattactcgg tattattttt tccaaactct
    30001 tttcaatgat tttggttcga atgatggata tggatcttac tagtccattt tttatctcca
    30061 ttccatcagt agtagatact ttatttgctt ttttcatcat ccttttagca gtatctgtat
    30121 attcaatatg gaaaatatgg cgctatccaa tgattcgaag ttttggagaa aaagaacaag
    30181 cagatagttc aacaatgcgc ttcaggacaa ggcaccgatt actcgcagtc atcgggttac
    30241 tacttttaac gagcggatat tttggagcta gtcattttag ggaaatcact tcatggatga
    30301 tcagttatca ttatcttgat acattagttt tcttttttcc ttttttaatt tttttcgttt
    30361 gtgtggtcgg tacttatttg tttttctgtt atacatttcg gttatttttg atcatattga
    30421 gtaaatggaa gatttattac tatggtatca attttttgat aatgggtaac acgcaagttc
    30481 atttgctcaa aagttggcgt acaaattctc tgattacagt cattctcgga atttctttaa
    30541 tgacaattgg cggcacggct agtatctcta ctattttatc ctaccaggag aaagtttcct
    30601 ccccgatgga ctatcagttt gatgttgaga cagagaaaaa aatcaagccg attctagcag
    30661 aggaagggca gaagataact aaagaaatca ccttgcatta taaagtcatt gggagtatct
    30721 atagtcaaaa tctcgaagaa cctatgtttc aaactgctaa tctgatcacg gaaacacaat
    30781 atcaagcctt cagaaaagtt aatccagatt tgccgaacat ttctttgaaa tcacctggtc
    30841 atactgtttt gcttgatcaa atgcaaactc tttttcggaa tatttcatta tatggatcta
    30901 gtattggact gccagataat caagaattga agattcaaca aatgcttcca agtgtattag
    30961 gtgacgaaac aatggtatat ctaagtcctg tactgattgt agatgatacc gtttttgaaa
    31021 aagcaaaagg tgtggattat caagtagtca atgtagatgt atcaggtgga aataatgaaa
    31081 aaatagatga acgactgagc aatgacctgt cgataaagtg gctcaatgct gtttattatt
    31141 cttatgatat cgtaaatgga agtctacaag gagaaattag cacaaagaaa cttccggatg
    31201 accaaatgaa ggaagcacaa tttagccaag aaagctctcg tttgaatttc tcttccagct
    31261 attcgaaatt gcgtgcagtt aatcggcgaa tcggtatcaa tatttttgtc acactatttg
    31321 tcgggatgat cgtgattatt gctactggta gtattttgac agtccggcaa ttagcagaag
    31381 cagaggaaga aaaagaaaat tatcacctat tgactaagct ggggattcca caaagaagga
    31441 tcagacggat gatcttcgag caaaacgcac ttaccttctt tccacccatg atattaggga
    31501 tcactcacgc agtatttgca atcaatgtct tcactcagta tataaaaact gccgattact
    31561 ggttggctta tcttgtgagt ggcttattga tccttatcta tcttttgctt tatgtcatta
    31621 cgagtcagtt atattgtcgg attatcgagg aatgatatac ggaataagac aagataataa
    31681 atagaactaa attttgaata ccatttattt ggaataagag gttgtgacac gattt
//
LOCUS       DACDIG010000027        30003 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.027, whole genome shotgun sequence.
ACCESSION   DACDIG010000027 DACDIG010000000
VERSION     DACDIG010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.027"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            173..511
                     /locus_tag="IV247_001340"
     CDS             173..511
                     /locus_tag="IV247_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777419.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(716..955)
                     /locus_tag="IV247_001341"
     CDS             complement(716..955)
                     /locus_tag="IV247_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAP8777420.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(931..1278)
                     /locus_tag="IV247_001342"
     CDS             complement(931..1278)
                     /locus_tag="IV247_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAP8777421.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(2010..2591)
                     /locus_tag="IV247_001343"
     CDS             complement(2010..2591)
                     /locus_tag="IV247_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8777422.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2774..3400)
                     /locus_tag="IV247_001344"
     CDS             complement(2774..3400)
                     /locus_tag="IV247_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8777423.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3422..4753)
                     /locus_tag="IV247_001345"
     CDS             complement(3422..4753)
                     /locus_tag="IV247_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAP8777424.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(4908..5309)
                     /locus_tag="IV247_001346"
     CDS             complement(4908..5309)
                     /locus_tag="IV247_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP8777425.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5395..5601)
                     /locus_tag="IV247_001347"
     CDS             complement(5395..5601)
                     /locus_tag="IV247_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777426.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(5696..7198)
                     /locus_tag="IV247_001348"
     CDS             complement(5696..7198)
                     /locus_tag="IV247_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAP8777427.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7216..7671)
                     /gene="argR"
                     /locus_tag="IV247_001349"
     CDS             complement(7216..7671)
                     /gene="argR"
                     /locus_tag="IV247_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP8777428.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(7769..8386)
                     /locus_tag="IV247_001350"
     CDS             complement(7769..8386)
                     /locus_tag="IV247_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777429.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8607..9980
                     /locus_tag="IV247_001351"
     CDS             8607..9980
                     /locus_tag="IV247_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP8777430.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10055..11167
                     /locus_tag="IV247_001352"
     CDS             10055..11167
                     /locus_tag="IV247_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAP8777431.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(11239..12876)
                     /locus_tag="IV247_001353"
     CDS             complement(11239..12876)
                     /locus_tag="IV247_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAP8777432.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13044..13652)
                     /locus_tag="IV247_001354"
     CDS             complement(13044..13652)
                     /locus_tag="IV247_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8777433.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(14036..15187)
                     /gene="queG"
                     /locus_tag="IV247_001355"
     CDS             complement(14036..15187)
                     /gene="queG"
                     /locus_tag="IV247_001355"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAP8777434.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(15206..15958)
                     /locus_tag="IV247_001356"
     CDS             complement(15206..15958)
                     /locus_tag="IV247_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777435.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(16099..17049)
                     /locus_tag="IV247_001357"
     CDS             complement(16099..17049)
                     /locus_tag="IV247_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP8777436.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(17050..17889)
                     /locus_tag="IV247_001358"
     CDS             complement(17050..17889)
                     /locus_tag="IV247_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP8777437.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(17886..18632)
                     /locus_tag="IV247_001359"
     CDS             complement(17886..18632)
                     /locus_tag="IV247_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP8777438.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            18870..20240
                     /locus_tag="IV247_001360"
     CDS             18870..20240
                     /locus_tag="IV247_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8777439.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(20295..21191)
                     /locus_tag="IV247_001361"
     CDS             complement(20295..21191)
                     /locus_tag="IV247_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8777440.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(21472..22149)
                     /locus_tag="IV247_001362"
     CDS             complement(21472..22149)
                     /locus_tag="IV247_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP8777441.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(22240..23145)
                     /locus_tag="IV247_001363"
     CDS             complement(22240..23145)
                     /locus_tag="IV247_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8777442.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            23320..24774
                     /locus_tag="IV247_001364"
     CDS             23320..24774
                     /locus_tag="IV247_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8777443.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            24790..25821
                     /locus_tag="IV247_001365"
     CDS             24790..25821
                     /locus_tag="IV247_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP8777444.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            25828..27015
                     /locus_tag="IV247_001366"
     CDS             25828..27015
                     /locus_tag="IV247_001366"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAP8777445.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            27171..28553
                     /locus_tag="IV247_001367"
     CDS             27171..28553
                     /locus_tag="IV247_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP8777446.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            28680..28910
                     /locus_tag="IV247_001368"
     CDS             28680..28910
                     /locus_tag="IV247_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777447.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(29110..29778)
                     /locus_tag="IV247_001369"
     CDS             complement(29110..29778)
                     /locus_tag="IV247_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAP8777448.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT         8415 a         6143 c         5477 g         9968 t
ORIGIN      
        1 agaaataaat tgtagccagt tcattttttc tttaactaaa aaaataatcc aagaaacatt
       61 gaacctatgg atccaaaagc tacccaaata ctatagctaa ccccccgcta ccttcaattt
      121 tgcagataaa acgctttggg agaaaatttc aaagacaaca ggccatccta tcatgcccga
      181 ttataaacaa aaagctggct attccttttg ctctgacaaa tttcaattgg gaaagttcac
      241 gaaactagca ggtgaagaag tttcaactgt tcggatcaaa gaatgcccgc ttcaaataga
      301 aacattggtt caacgaattt caactagaca agactttgct attgtagaac gtgagatcgt
      361 agctattttt gtagaagaag ggattttaca tgatccaact catatcaatg tggataaatg
      421 gcaacctctt atctataaat tcagagaata tacaactgcc aaccagtcgt taactttgaa
      481 ttttagattc caagaattca aagagtcata atgataaaca tcgttaggta taaataaaaa
      541 agactcgcag cacgagtaaa aaacaaatag aagctataca ttagaaaggt cgactgtgac
      601 cctatagatg cagtatatgc agcttatgag catttcttct gttctctttg tttttgggat
      661 ctgtgctgaa gtctttttcg ttttttgccg ctctttcttt gaaaaatcga ttatgttata
      721 ccttctccat tactaattca ggcatctgct gagtaatgat ttttcgcgca aaagaaatat
      781 ctgttaccca aggttcgatt tgttcttcag tcaagacaag cggcattcgg tcatggattg
      841 gtgcgactgt gcggttaggt tcagtagtca ttatgattga ttctgccatt ttttcatcct
      901 gatggctacg ataaaaaccg ccaatataca ctattttttc cttatttgaa aacataaatt
      961 tgtctttttc ttcattccac tcattgggaa aacacaacga ttttgcaaaa atggttgatg
     1021 aaaggtcttt ttttcttcta ctgtctctgc acgggcatta atcatcaatt gtcctttttt
     1081 aaatccggta aatccccact tcatgatttc cggagtgagg atgttttctt cctcttttct
     1141 taacgtcaca acaagatttg acggaaaaac ttctccagct gctaattctg ctgcctgctt
     1201 cggttgtttc gtctgggctt ctcgataata attctgaagg agctctgact ctagatcaaa
     1261 aaaataacga ccacacatcc taatccctcc caatcttttt ttctatcata cactgcaaca
     1321 agaagttcgc accaacaaaa cgctttcaat ttttgtagaa ctaaaaaagt tgtgacaagt
     1381 gacaaactct ccagtgaatg gtattctcct gccaactcct cggtcacaag tcacaatatt
     1441 caacaaacat gagaagctgc tttttgaaat tgttccttgt gactcggagt gaaacggcag
     1501 gcttacgacc tctgggtaaa cggtgttcaa aagccagttt ctcggtatta agccaaattt
     1561 atgcaaaaat gtagaaagct attttcacaa aatttttctt aatactcgaa actaagcgac
     1621 tttttcacaa cctcttcctt aaacggtgtt caaaagcttg catctgcggt aataagccca
     1681 accaaacgaa aaatatgagc agctattttt atttggctgt tcttattact tgatgcaggg
     1741 cagctttttc acaacctctc cactaaacga tgttcaatca gctaaccttc ggtattagct
     1801 caaaaaacgg caaaatatga ggaactattt tgccgtttgt ttctctaata ctcaggtcaa
     1861 agcgctgatt tcacaacctc taatacacgg tgttccagaa gtagcttctt cgaaaaataa
     1921 gccgaaattt cacaaaaatt tgagaagcaa ttttcgtaaa ttccttctta tttcttgaag
     1981 ctgaccactt ctgtcacaac ctctaattat taaagaacct tttgcgaagt taacaatctt
     2041 tctttgatca attgaaaatt ctgcctaagt agtgatagat cctgtgtgac tttcaacaat
     2101 aagatacttc cctggatttc agcaatcaac gctttggctt gacggtctgc ttcttcatcg
     2161 gacagttttt cttcagaaag tgccaggata ttttgtttcg tatacttgac ccattgattg
     2221 actaattcta gtagcaacag acggtattct tcatccactg tactcaactc aataatcaaa
     2281 ttacctacag gacagccatg ataagtgatc tgtgtctcat gatcagcgaa aatccagtcc
     2341 aacatttctt ctaatgcttc ttctggctgt tttccagcat ttaagatgtc ttcgaacaat
     2401 tctgtctgcc atacgtccat atgcgcatgg atcaccgcta gacaaacttc tttttttgaa
     2461 gaaaagtaat aataaaattg accttttcct gtatcagaag ctgctagaat atctcggatt
     2521 gacgtttcat gatagccttt tttataaata agatctttgg tcgcttccaa gatcttttcc
     2581 tttgttttca atgatattct cctttttcat ttctcatagt cctatattat acagtaaatt
     2641 catcattttt tcttcttaat cgtttaaaaa agactgtgcc aaattatctg acacagccct
     2701 cactataaaa ctgttctcat atagctagat gttcattgat tcaagaactg cgaggacaaa
     2761 agcaattcct atcttatgat agttgctcta atacttcctc tgttgtcatc acttgatctg
     2821 caataaagcc aaagttcgtc aacgctgcat ccattgcttc ttggccagga gcaccaattg
     2881 catctgtcgc taccacaaca ttgaatccac tctctaccaa ttcacgcata tgggaatcgc
     2941 tacacaagtt tccgttcatt ccaccaagaa ttactgtgtc gattccattt tttctcaatt
     3001 ggagcgccaa atcattgctt tctggtccaa agattttatg aggagagctg atgatcgtat
     3061 tttcatccaa ataaggtttc aattcttcga taaaatcggc tcctgaacct tcttccactg
     3121 attcatattg actttttcta gcaaacatct gctgctcaag catcatatgt tctcctgcac
     3181 cgccaaattg ccatttatgg tcatgtgggt aaaaataatg aggagaaata aagagtttgt
     3241 atcctttttc ttttgcttgt tttaccattt caatcatatt ttcgatcgta ttgtattttt
     3301 ctaagatatc ttttgtcaaa ccaaagccac cgcctgctgg tttcaagaac tcgacttgcg
     3361 ggtcagtgag aactactgct gtatttttat tattgaacat ggtggattct ccattcgtta
     3421 tttattttaa tacatcattt agagcttcac tcattgtcgg atgagaatag atttgatctc
     3481 tcaataacgt ataaggaagt tttccattca tagctagcga aatcaagttg atgacttcat
     3541 gagaatcttc cgcatagata cttgccccta gaatcaagtc agtttcaggg tcgactaaaa
     3601 ctttgaatac acctttagga tcttctaata cttgtgcttt agggacaccg cttgtcatat
     3661 acttgaataa tttgtaattg acgccttgtg ctttggcttg tttttctgtt aaacctacat
     3721 tggataaagt cggagtgata aatacactgt aaggcacagc tttccggtta ttcgttgttc
     3781 gtttattctc gcccttcaac tgatccataa tgattcgata atcatctaga gagatataag
     3841 tgaactgcaa tccgcctttg acgtctccaa tcgcccaaat gttgtctgca tctgtacgaa
     3901 ggtattcatc cacagcgatt gctccgcgat ctgttagttt aacatctgta ttttctaatc
     3961 caagtgatgc tgtacttggt tttcttccag ttgcagccaa aacttttgac gcttggattg
     4021 ttacttcttg gccatcttct gtaaaggtga cggcagctgt tgattcttgg tctgctactt
     4081 gatctacaga tacacctaga tggaattcga ttcccgcatt tgtcatgtct tctaaaatca
     4141 tttgcgcgat atcttcatct tcacgtggaa gaaattcaga atgggcatca agaacgacaa
     4201 ctttagatcc gtattcattg aacattgatg caaattccaa gccaatatat ccagccccga
     4261 tgatcactaa tttttccggt aattgttcct gatccatcgc ttgcgtagaa tcaatcaagt
     4321 agcgacttgt attcaatcct ttgattggta aaatgattgg ttcagaacca gtattgatga
     4381 aaatccgttc gccttcaaca atcacttttt caccattatt cgtcacttcg atttgatggt
     4441 tagaaagaaa acgtgctgta ccgtccatca ctgtcccagt tgcctcatcg ctgatcatat
     4501 gataattttt ggcgcgaagc atgcccgtca atttttcttt ccgagcaaca gcctctgtaa
     4561 agtcgattcc tctttctcca ttgaaaatca atgatttaga aggaatacag ccgatgttga
     4621 tacatgttcc gccatacata cgtgttgact tttcaacgac taaaaccgat tcaccttttg
     4681 aagctaacgc cttcgctaaa gtcttaccgc cctttccaaa accgatcaca atattttgat
     4741 atttttccat catttttccc tcgcttcttt tttacctacc gattggtaca aaaatatgat
     4801 aaccgataat tgtctattct ccaaatcatc tgctttattt gtcaaaaaaa accagacaaa
     4861 aagcggtcct ttcacgtttt tagtctggtt cgatttcctt tattcttcta aacaatcgtt
     4921 acttctacat cgatatttcc tctagttgct ttggagtaag ggcagaattc atgcgcatct
     4981 tctgcaattt tttgggcttc ttcttcgctg atgccatcga tatgcacttc taatactaca
     5041 ccgatctgat agccgccatc atctaaatta taaagagaga cacgtgcttt tactgtggac
     5101 tctgctgtca ttcctgcttc tttgatcata tgttgtaccg ccccattgaa acaagaagca
     5161 taacctgctg caaataattg ttctggattt gttccttctg catggattcc tggaggagtg
     5221 attttcatat gcatcgagcc atttggtgcc tcgacttctc cattgcgccc gccatggttg
     5281 atcattgctg tttcgtatgc ttttttcatt gtactgctcc tttcgattga ccatttgcta
     5341 cttggctata attatagtgt atgacaagta aggcgctttc tacaaaaatt ctgcttattc
     5401 gaataccaga atgccgtctt ctactatttt tccaaaaaac aaaaaaatac tactcgtgaa
     5461 caacagcgtc atatttgttc caaaataaat agcaatcttt tctacataat cttttatatg
     5521 attgatttta agatacatat ataccagaaa gttgacccca taaaccaata ctgttaaaaa
     5581 aataaccata ttcgttacca ttttttcctc cttttgctgg tctccctacc agcactcgat
     5641 ttaaccatac cacttttttt gccgtccaag caactagcct ttcaaaaaga ctggtttatt
     5701 ctgctgttaa tgattcaaga tcagaaaaat gtccgaataa ttttgctaat tttactaacg
     5761 ccaacttgtt ctttccgaat tcatcatttt ctatctgtaa tactagtaac ggctcatgta
     5821 agcttaaacg gatcaaaaac cagccgtttc cgtatggctc ttctagatca tatcgaatac
     5881 cttcttcatt ttccgggtcg aagttcatcc ctttttcttc ttccaacagt cgcttcattt
     5941 cttctaatat cttgtttcct tgttcgcgtg gttcatcgct gaacaatttt aatcggattt
     6001 cttgtacttc tacgggctgt ttcaactctt tgatttgttc atctaatgtg ataccttttc
     6061 tttttaattc tggcaaaagc atcaggatct ttgcgatgac atatgcacca tcatccagaa
     6121 aatagttttc cttgaatgca gcgtgtccgc ttgtttcgat ggctagctgt gtatctttcc
     6181 caagacggtt aagttctaat gctttattga ttacatttcg atagccggaa atataacgaa
     6241 cttggatgcc tcctttttct tcaatgaatc tttgtagatg agaagaagta ggggaattcg
     6301 tcacgattct tgttcctgga tgctctttta atacgattgt acttaatact gcgatcaaat
     6361 tatttcgatt gatcactgtt ccagaagcac tgacaactgc agaacgatcc acatctgtat
     6421 cgaaaatgat cccaagatct gcttcatttg ctaaaacagc ctgcttgata ctttccatcg
     6481 cttctttatt atcaggattt gggatatgat taggaaatcg gccatctggt tccaaaaatt
     6541 gggaacctgt tgaatccgca cctaagctat tcaatacttt ttcagcaaag aatccaccag
     6601 caccatttcc ggcatcaaca atgattttcc aaccatttag tggtttgctt tctagtgttc
     6661 caatcccctt tttaattttt gtgactaaat cttgcgcata aggtgtcaaa aggtctgctt
     6721 tttctacgct tccagtttgt tcgcttttta ttttttgctg ctgtgatagg ataaaagcaa
     6781 tatcttcttt ttctgctcct ccttctgagg taaagaattt tattccatta taataatagg
     6841 gaagatggct agcagtcagc ataaccgcag catcgcacga gaactgttca aattgagtgg
     6901 acataaacat agcaggcgta gtagctaatt cgaaatcgat gacatgtatc ccttgttctt
     6961 tcagactttg gatcaacgcc tctttcagtg ctggtccgct tatcctactg tcatgaccaa
     7021 tcccaatcgt ccatttttta tttggatcgg ttgatttctt ctttttcttt atccagtttg
     7081 ctagtcccca gccgatcttt tttaccatgt ctgtcgtcaa gtttacttca tatccttcaa
     7141 ctggtaatgc gattcctcga atatccgatc cattttgcag cgctttcata tctttcatat
     7201 aaaagtcctc ctcatttatt ccctcatttt ttccagcttt tctgcaaatc gctgtgcttc
     7261 ttcttctgag cggcaaataa taatgaatgt atctacaccg gccatcgttc cgacaacttc
     7321 agggtaagcc acttcatcta gccaattcgt taccacatcg gcaccatttc gttcagtcaa
     7381 tacgataacc atgaactgaa cagactggat acgcaaaacc tcgcgtctaa ttgctgtacg
     7441 caaacgttct tcatttaaag aaacagatgg ctggttaaat aatgtatagc gtactatttt
     7501 gttttcattt tgacttttta ctaacttcat ttctcgaatg tctcgagaaa tcgttgcttg
     7561 agtcacatcg gcgcctgctt catgcagccg cgcaagcagc tcttcctgtg tcccaatagg
     7621 ttgttctgta atgatctggc ggatcagtgc ttgtcgctgt ttctttttca tattagacac
     7681 ctctcaatgt tgattatagc aaaagataag taatatttct tcatattttg gtatttttta
     7741 cttttatttt ttgttttcat tcaggttgct aacctttttc tattttaaca cgcaaaatcg
     7801 tcttcatttt ttcaggtgct gattttcttg gatcgctgat gtaaatctct ttgtgttctt
     7861 tagaagctct ttcatagcct tcttttgcga tgaatgcttc taatttctca aacgttgctg
     7921 gttcttcggc atagggcccg acgtgtaaga tttgggcaca aagaccttct tccatctttt
     7981 tgaatactac ttgttctttc aagtcttccg gaagtttgct agaacgctga atggcttctt
     8041 gtgctatcgc ttctgtaaca aagtcaggct gtcggatcat caattgataa gaaaaatgtt
     8101 cttttttcat cactggttca tgcaaatact tttcttgcag tccccattgt ccttccagtg
     8161 gatagacagt atacatctga taatcaggaa tcagccaatt ttttttaggc gacatgcgta
     8221 ttgcatagct gactgcatat agacaggcta ctcttcgttt aaagtcttcc tgattcgggt
     8281 cgccgatccc tctgatgcta aagaatgatt ttttaggaac ctctagtatt tgcgggcttt
     8341 ttttcatact atagctcttg tctttacgcc attcaatttt ttccattttt cttcctccgc
     8401 gaaattccat ttagttattt attatcatac ctctttttga gtgacttggc taatcgtgtt
     8461 gacactattt ttcagtttgt gctagaataa gtgaagtaaa taaatcgtac agataacacg
     8521 tttcggcgtg tcgagagaga ggcggtttct atttttataa tagaaatcac tctctttttt
     8581 tacgaaattt tttagaggtg atgtaaatga caaaacaaaa acagctgctt ttagtacctg
     8641 cagtaattgc aacagggatg atgtcttttg cgggcgtatt gatcgagacg gccatgaatg
     8701 taactttccc aactcttatc agacagtttg atttaacgac tgcacaagtc caatgggtaa
     8761 caacgattta tcttttgatg atttcgatca ttgttccgct gtctacttat ttgaatcgca
     8821 attttagtct caaaaaactc tttttagctt caaatctgct tttcttggca ggtataatga
     8881 tcgatttctt ctctcctacc ttcatggtcc tattgtttgg tcgattgctc caaggtgcag
     8941 cgacaggtat cgctcttccg ttgatgttcc atattatctt gacatttgcc cccctcaatc
     9001 gacgaggcgc gatgatgggt gtcggtactt tgacaacggc cattgctcca gcaattggcc
     9061 ccacttatgg aggcattatg acttcacatt ttacatggaa tcatatcttc ctgtttcttg
     9121 tgcccgtttt gtttctttct ttgattatcg gctggtttgc tattcccgag atggcagtac
     9181 aaaaaagtgg ttatttggat tggataagcg ttttaggaat ttgtcttttg tttagtggaa
     9241 gtctaacatt tttgagtacg ttgggaacag tttccagctg gattgcttta atcatcggcg
     9301 gtattggctg gttccttttt tactatcgaa gtaaaaaagt ggagcatcca ctgatccgac
     9361 tgtctattct ttcaaataaa acattccgct tgtttttgat tggcttctta gtttatcagt
     9421 ttttattact tggtgtatct tttgtcttgc cgaatttcct tcagatcgtt caaggaagta
     9481 gtccttttgt cgctggatta gcgatgcttc ccggcgcagc ggttggtgct ttgctttctc
     9541 ctttttccgg gaaaatgctg gatcaatatg gtccgaagaa gccaattttt gctgggctga
     9601 ttctttcttt gattggttgg ctagcattat tcctactcat aggacacgct tcagtgggtg
     9661 tattgattgc ttgtcatgtg ttttatatga tcggtatcgg attatcttac agcaatatga
     9721 tgaccaccgg aatgaatgca ctgaatgaag aactacaagg agacgggaac gcaatgttca
     9781 ataccttgca gcagttttct ggcgctgtgg ctacttcatt agtagcggtc attatcaatt
     9841 acgtccagca tcatacttcc cataactatg aagtgtctac tacattagga tcaaaagcag
     9901 cattaggcgt cttgttgctg ttgcttctcg taagctttgc tcgattcgcc tattatctgt
     9961 tctttgctaa aaaagcgtag attccatata cggcaggtgt tgcatctgcc gtttttttgt
    10021 gtgaaaatgg ttgtttgtaa aaggaggctt ttttatggaa aaaattgcca ttatcggtgg
    10081 cgggatcatt ggtatgaccc tagccagtta cttagataca tcaaaattca atgttgcttt
    10141 atttgatgaa gggacaggac aggcaactaa agctagcgct gggattatct ccccttggtt
    10201 gtcgaaacgc agaaacaaac aatggtatcg tctggctaaa gacggcgctg cttttttccc
    10261 taaacttata aaagatttcc aacttgatca aacgatttac gcacaatcag ggacactttt
    10321 gcttcgacct gaaaaagatt tagctgaatt ggctgttctt gccgagaaac gaaaagaaga
    10381 agcgccagaa attggaaaca tcgaatggct ttcacctgaa cagacatctc aagcacttcc
    10441 gttattgaaa ccagcagttt cactgcgtat cagtggtggt ggtcgtctgg atggaaaagc
    10501 atatttagaa catcttggtc agcgtttagc acaaaaacaa atggagcaga tccaggcaaa
    10561 agtcactttg cataagaaga gcgattcttg gctcatcaag ggaccgtttg gagaatacac
    10621 agctgatcgt gtaattttga cacctggccc ggcacttaag cctttattac aagaattagg
    10681 ctatgctgca gatatacgtc cgcaaaaagg gcagctctta gcatttcaga catcttataa
    10741 gatgagtcaa gactggcctg ttgctatgct tgacggagaa gctgatttca ttcctttcat
    10801 ggatggaaag atccttttag gtgctaccca tgaaaacgat caaggctggg atttggctcc
    10861 tactagcgaa gcttataagg gattgactaa gagtacagaa cagttcttga ctgatccaga
    10921 tcagataaaa aactggccat acgagttccg tgtaggtacc cgtgcttata cgtcagattt
    10981 ttccccattt ttcggcgtac tgccagatga tccaagctta ctggctgcta gcggtctagg
    11041 ctcttctggc ttgaccacag gtccttatat cggctatcta ttagccaact tcttaaatac
    11101 tggtcattgg cctacggaag attatcaaaa ggacatctcc acctatatta cgaaaaatcc
    11161 atcatagaaa aacagctttt tcaagtaagg atcccgagcg atattgcgca tggattactt
    11221 cttgaaaaag ctgttatttt agttcaattt ctgaatcaaa ttctccaaac tttcttttgc
    11281 ttcatctggc aagagatctt ttcctatctg agcttcaatg aatcgtaaac gaatatcaag
    11341 tcgttgtttc accagatttt tataggacgt atcctcaaaa gaaacaggaa aatctggtat
    11401 aggtaaatag tggatgtctt caaatggacc aaacgacttg aattctgccg tttgttgcac
    11461 gattctttca acgattgaca atgtcgtttc aggtttgatt tttttatcat cgaaatatcc
    11521 agtattcagg acatagcagg ctgtttgctg tttcaaaaac aactctcgaa aagcttgata
    11581 gtcttcccta agaggataga cacggaaagg attagcaaat ggttcaatga ctagctcatc
    11641 tagctgtctt tttgtagtga tattttccgc gtctgatcgt tttgttgcta atgttgcacc
    11701 aaaagacgct gctaaaactg ggtcattgat cttgataatt ggcggaagcg aatcatcttt
    11761 catgatccaa aaaacagcat cgatcttttc gctaagatga tcgactcggt cgggtgtcgc
    11821 ataacgtgat ttgactgtcc gcccatttcc gtttcgtata tctcctgtta ctaggacttt
    11881 ttctccttta cttgtacatg tcacgcccac attctgacaa gtaaggaaag aagaaatggc
    11941 cggatcatca gacggatagt cttgtgtttt atcaaagtaa gaaggttcca aggcagtagc
    12001 aaaaccattt tttcgagaaa taacgaaagc atcgtcatgc aagatcgaaa cagagtgacc
    12061 ttccgtatgt tttgctaagg taatcgttga ttttccagaa ccggacaacc caaatacagc
    12121 catcgtatag gtctggtcat tactagcata ttgtttcatc ccaccgtggc aggcaataaa
    12181 gccattgcga tttcccaatg cccaagcaag cgtcaaagtc gcttttttta attctccaaa
    12241 gtaacgtaat cctagcacag ccgctgtatt atggagagca tcaaacaaaa tgagtccatt
    12301 tggaaatgtt tcatgtgtcc attcgggatc acaaaagata taaagatctc cctcatcaaa
    12361 ggaaacagac ttttgataag tctctaagat cttcgaattc gctggctgaa aattcagcaa
    12421 ataagaatac aaattgttct caaatccttc tggaatcagc aaatgacttt gcacagaaaa
    12481 ttcttcagat aggccaacga ttacttttgt atggtaaaac tggcgcttgc gactttgata
    12541 cacagcttct cttaaaattt tctcgtattt gctttgttcc ttgtctaagt cctcaaagat
    12601 ccgccgagca cttgctgttc ttcccacaat gcttccatca ttgaatacta gtgtcctaac
    12661 atcagctggt aaatctagtt cttcagcatg tgagattgtt aaatctgtga tgatcgttcc
    12721 tggactccct tttgctagtt gataagctgt cttcctatca aaaacttcat gaacattatt
    12781 accgtaaaat gtcgtttcta caatcgatcg aatactggat aacagtggat ttccacttgt
    12841 gatttcgttt aattcgagac tctcttttgt actcatggct cctcctcatt ccctaaacaa
    12901 aaaaacgttt tctttattat acattattcg agtttttatt tgtactgctg cgctgtggga
    12961 agcaaacgag ttatttttca cacaaaaaaa ccgagaactt gaataaacaa gtttctcggt
    13021 ttcatcattt tccttttatc ggcttagtac cagccgtttg ctaaccagaa tgttttagca
    13081 gcatcccatg aaccataacg agatgttaca tattgttctg ctacacgttc ttgattggca
    13141 gctgaatagt cgccgtttaa gtatgaagca tctaattgat aacggccgat gtaacgtccg
    13201 ttagtagctg tgtatgaacc gcctgattct ttttgtgcga tccattcttt tgcagagctt
    13261 gaagttggcg ctgttgcagc agctggggca gcaggagcta cttcaactgt ttcaacaggt
    13321 gcaacgtatg tatgttcttg cgttgctgtt tcagttgtag atgctgtatt ttctgttgtt
    13381 ttgttttctg ttgttgctga agaatcattt gaagttggga ttgttaattg ttcaccaaca
    13441 taaatgcggt tgatgtttgc gatcgaattt ttttcagcaa ttgcatcgat cattgagttg
    13501 tctcctgcaa atttttgtga gatcttagat aatgaatcac ctgattttac tgtataaact
    13561 tcatcagcgt gagcatttgt agttcctaca aacattgcag tagcaccagc agcaaaagtt
    13621 gtacctaaaa caattttctt aaatgaattc atattaaact cctttttttc tccgtttttt
    13681 attctttcga cgctattaac tttatcattt agaaatattt cataggtaaa agaaacatga
    13741 catttaatga caaaataacg aattatttaa tatttgtaac atttaaacaa atattttttt
    13801 gatacaaaac cataaaagcc ttgttgtata agcaaagtaa tcgtttttct atgttttttt
    13861 atttttgaat gaaaaacgca gattctgtaa cattcactgc aataatccat aaaaaaaagc
    13921 aaaaaaattt ttgattttta tcaaattctc ttaaaaaaat gtaaaaaata caaaaaaacc
    13981 agtaattgtt acaacaatta ctggacaaaa actttgaata atgttaacta tttttttatt
    14041 ttaactttct ccgcaataat ttctgggcat gttccaattc gtttataacg acttcttctg
    14101 tttctttagc taatatttca tctaatacat caagccattt ttccggttct tttgttccta
    14161 gacgtccaat agcccaagct gctgttcctc tgatgacagg gcggacatct gttaaacatt
    14221 ctgaaatctc aggcaatgcc gttcgatcac ctagattagc taaagcaatc aatgcgtttc
    14281 tctgcaaagg tttcttccca cgccatgaac cagctaaatg cccaaattgc tgtttgaagt
    14341 ctttattaga catcgaaagc attggcttta attttggata tatttcgtct acttttggtt
    14401 ccatctcttc atgaaaatga aaatcttttc cttgattgta agggcagact aactggcaga
    14461 tatcacatcc ataaatgaca tttcgcattt ttttacgata ttcttccggc atcatccctt
    14521 ttgtctgagt ttgataagac aaacatcgct gggcattcat tcttccgtca ccaagcaatg
    14581 cttttgtcgg acaagcagtc acgcagcgca tacagtcacc gcaaccaaat tctcctggtt
    14641 tatcaggctc aaatacaata ttagtaacga tctcaccaag atagacaaat gaaccgaact
    14701 cttcagtgat caacaatccg tttcgtccaa taaatcctaa accagctcgc tgtgctacag
    14761 cgacatcgat cagttctcca gtatctactt gcggagctag acgccattcc tcttcttttt
    14821 gccatttttc tgctcttgtt tggatgaatt cgatcaatcg gttcaaacgg tctctgagta
    14881 tatcatggta atcgatcccc caagaagcac gagcaaactg cccgcgtttt tcatccctag
    14941 gcacttcttc atggatccta gtaggatacg caagtgcaat ggaaatgatc gatttcggct
    15001 gatcaaatgt catttctgga taaagacgct catcgatatt tggatgttca aagccagaag
    15061 tatggccagc tgctttttgt tcttccaacg attcttttaa tgaatcaaaa ggttccgccg
    15121 tggtgaaccc gattttgtca attcctaatc gtttgctttc ttgtatgatt tcttctttta
    15181 atgtcatggt cttcctcgct tgctgttaac ggctgtataa cccataatat agtttttctt
    15241 ttttcagctg gacaaactgg atctgctgat cggcaatctt cttttcttcc atccattttt
    15301 ttcttgcagc aaatacttcc caataatttg ttgaaattaa ttttcttttg ttatctaatg
    15361 cattaaaaaa attcaatgaa actggttttg tacttaacag ttcatggagc cttacctctt
    15421 cccatggcga aaactcagac agatcgcggg aatacaatac ttcttcatag agattttttt
    15481 gctgtgtgta cgtgaatgtt tgcgggattt tcccaggcct agcaatttct actagacgat
    15541 tataaaacat attcattaat ttgctgttgg caataaattg acttttataa taggccgact
    15601 ggatcttttt gattactcct tctgctttga tgactgaaaa taatgcaggc catgctttga
    15661 cggttatttc gtcgttttgt ttaactaata cgccaggtgt gaagccgact ggagaaagga
    15721 acacaaaata accgataccg gtattttcat ctaaaggctg aaattcatca aacaaccatt
    15781 tgcctatgta cagctcttca tctggttctg ttgtctcatt ttgatttgct atgaataatt
    15841 tagctaattc tgcttcaaga aattgaaggt cttgatcctt gtcggtattt tcctgctggc
    15901 tgatcaatcg aaagacttct tctttaatcg tatatttatc tttacgtgta aaattcatag
    15961 ccacaccttc cttccttact ctaactataa cggaatttct tcaaaaaagg tatctgtacg
    16021 acgaaaaaca gcataatttt ttttcatatc tcttataaaa aatgcaaggt ctgctcctca
    16081 agctagacct tgcacttttc agctttcttt tgtttgtgac aaaccttcat ggatcgtttc
    16141 aatattccat ttcatcatct gatagtaaga atctccttgt tctccttctt ttgctatgga
    16201 atcagtgaac agtttatcat agattttcaa acccgtttct tttgctaccc gttccatgct
    16261 tcttgagtct acactggttt ccacgaataa aaccggtact tcttttgctc ttatctgatc
    16321 aatgatcgct ttcatttgat caggcgtacc ttgactttct gtattgatct cccatatata
    16381 agctgctggc aaatcatatg ctcgtgaaaa atacttgaat gccccttcac ttgttactaa
    16441 taattttttg ttctcttcga tagaagcaaa actttccttc gcttgtgtat ctaattcttt
    16501 tagtttttcc acatatgctt ctgcattttt tttatagagt gtcgcatttt ctgcatcttt
    16561 ttcagagaat atacgtgcga tttcctccac atattttatt ccgttagata ggtctagcca
    16621 agcatgggga tctgcttttg tatgttcttc accgctagtt aaataaagag gttctacatt
    16681 tttgctaact gcaaaataat cttttccttc ttctttttta gccgtttcca tcaagttatc
    16741 gaaccagctg ttacctgttt caagattcaa accgttgtat aaaataacat ctgcatcgct
    16801 tgcctttttg atgtcttctg gtagtacttc atattcatgc ggatctgttc cgactggtac
    16861 gatactgtgg atatctattt gatctgttcc gacctctttt gccatgtccg ccaagatcga
    16921 attggtagcc acgactgaca acttttcttt acttccttca tccgcttggt cagaagcctg
    16981 ctttccgcaa ccggctaata ccaagaaact aactgccaag attaaaaaga gtgatttttt
    17041 tgttttcatc tatctgcttc ctttctgaaa taaaattcct tttgttggag aaaataaaaa
    17101 agcaaggata aaaaatgcgg tacatgctaa cacgatcgta gctcctgatg gccaattgta
    17161 actataactg aaaaacacac cgacaactgc acttagcata ccgattcctg cagctgtaat
    17221 gatcattttc agtaaatggt tcgttagcaa gtaagccgtg gcggctggtg tgatcaacat
    17281 ggcaatcacc aagattgttc ctactgtctg taaactgact acagccacta atgtcaaaaa
    17341 gaacatcaaa agataatgga tcagcgatgt gttcagccca taagcttttg ccattgtcgg
    17401 atcgaatgat gtgatcttca attctttata aaagaaaaag acaaatatca agacgattcc
    17461 actaacgagt gctgtcagca acaaatcgct ttccctaaca gctaagacat tcccaaataa
    17521 aatatgatac aaatctgtcg cactctgggc atatgaaatc aagataatcc ctaatgcaaa
    17581 aaaggagcta aagacaattc caatcgctgt atcatttttc aatcggctcc gctgagtcac
    17641 gaaaccaatc actgctgctg ttagcatccc aaaagcagtg gctccaaaga tgtaactaaa
    17701 accaaacata taagaaatgg ctacacctgg aagaactgcg tgagagatag cgtctcccat
    17761 caaagacatc cctcgtaaaa taataaaaga accgatgact ccagaagcaa gtcctacaag
    17821 catggaagtg accaaagcgt attgcaggaa ttgataattc attaatccat caataaagga
    17881 ttggatcatt ttttctctcc tccttttact aaatgtctga tgatctcacc ataggccaat
    17941 tgaaggttct tcgatgtaaa gacttcttgt actggaccgg aagcgatcag ctgtttattt
    18001 aaaagaataa cctcatcgaa atattcttct actttatgaa gatcatgatg gacgatcaaa
    18061 atcgtttttc ctgaattctt caattcctgc aagatgtcaa agatctttcg ttcacttaac
    18121 gcatcaatcc ctacgaatgg ttcatctaaa aagatccatt ctgctccttg ggctagagct
    18181 cttgcaataa aaactctctg aagctgtcca cccgatagtt cgctgatctg tcttttggca
    18241 tattcttcca tccctacttt tttcaaagct tgtcttgcac gttctttttc ttttttccca
    18301 ggtctttgtc caattcgtaa agatggatat gttcctaaaa gtacaacgcc taatacatca
    18361 attggaaagg aaagatccaa ttcacttcgc tgttccacgt aggctatttt tttacggact
    18421 gagtttaccg actgtccaga aaaggtgact ttaccagtag tagctggtat caactctagc
    18481 aaagatttca taaatgttga ttttcctgca ccgtttggtc cgataatacc ggtaattttt
    18541 tgtggattta tcgtcaagtt gatagttttc aaaacagttt tcccttgata agcggctgaa
    18601 aggtcttgaa ttacaatggc cgtacgttcc aaatcttaca ctccttttta ggtttaccta
    18661 actttttatt taaatatagt ttaactaaaa tggaaagcat tttcaagtag aatgatgaaa
    18721 aagttttttt cgaattttcc tcgcatttta tgaaaatttt ctgtagaatc acatcaagag
    18781 ttcaataagg ctaacctaat aatgaaaaac atgtgttgaa acgtttgatt attttttctt
    18841 gttgactcaa caacgaaagg agtttttgta tggaagaaga aaagttaact cgaagtctta
    18901 gtgcccgtca tatccaaatg atcgcactag gtggaacgat cggtgtcggt ctgtttatgg
    18961 gtgcctcctc aaccatacgt tggacagggc cctcagtgat gggagcctat gcgattgcag
    19021 gattcttttt gtatttgatt atgcgtgctt taggcgaaat gctttacgtt gatccttcga
    19081 ctggttcttt tgcaaattac gcgagcgagt acatccatcc tgtggctggc tacttgaccg
    19141 cttggagtaa tatttttcaa tatattgtcg taggaataag tgaagtgatt gctgtaggat
    19201 cttatatgaa ttattggtgg cctgatttgc cggcaattat tcccggaatc attgtcgtct
    19261 tgtttcttat gctggcaaat cttatttctg tcaaggcgtt tggcgaatta gaattttggt
    19321 tttcgatcat caaagtcata acgatcattt tgatgatcat tgcaggtctt ggggtaatct
    19381 tatttggatt tggtaatcat ggtcaggcag tagggatttc aaatctttgg aaaaatggtg
    19441 gattcttcac tggaggcgtc aaaggatttt tctttgcatt atcgatcgtt gttgcttcct
    19501 atcaaggaat cgaactgatc ggaatgactg ctggtgaagc agagaatcct aaaaagacga
    19561 tcattgaagc tgtccagtcc actatcggtc gaatcttgat tttttatatt ggtgctattt
    19621 ttatcattgt gagtatctat ccgtggaatc aattgagtga gattggttca ccttttgtac
    19681 aaactttttc taaagtcggc attacatttg ctgcaggact gattaatttt gtcgtgatta
    19741 cagcggcatt atcaggttgc aattcaggga ttttcagcgc aagtcgtatg gtctatacac
    19801 ttgccataaa tggaaagatg tctaaaaaat ttctgaaact atctcgtcac ggtgcgcctt
    19861 tttatcctgt cgtagcaatc tcaggtggaa tcttaattgg tctgatctta aattatcttc
    19921 taccaatgtt catcaaccag tcagaaaata ttttcgtttt cgtatacagc tcaagtattt
    19981 tgcctggtat ggttccttgg gtagtgattt tgatcagcga gattcgcttc aaaaagaagc
    20041 acgcagataa aatgcatgat catcctttta aaatgccact atctccttat acgaattatg
    20101 tgacacttgc ttttttagct atcgtattga ttttcatgtt tatcaatcct gaaacgagaa
    20161 tctcattgat tattggactc gttttcttgc tttacatgac gatccattac tttgtacgcg
    20221 aaaaaagaac cggaaaataa aaaaagaacg aagcttcacc ttagtggaag cttcgttctt
    20281 ttttattgct tttatcaatt caattttagc gtctcaccgg gagtcaggaa ataattctcc
    20341 atgctcatcc cattcagcga caagatcgtt tctactggta cgccagttcg ttcggctatt
    20401 tgatttggac cttctccttc caaaactacg gttgtcgcag cctgtgaagt cgcgctgttg
    20461 gtattagttt cagaggaatc tgttgtgtct tgtgttgttc gagcttgcat ttcagaagct
    20521 tctaccgact ctgctgtctc agaatcagaa gatgttgtgt cttgtgtgct tgttgactct
    20581 gcttgaagag aggcgctagt ctgcaaataa atatcggaaa tcttagcgac atgattcgta
    20641 taattagccg ataattgatt gatcaaagta tctaagtcta caccgtcttt attttctgct
    20701 gcctgatacg ctgcacgtaa ttttctgatc ccttcgtcgg agattgcttg aagctcacgt
    20761 gctttttggt tagcaactgc tgataaacca gctattccct cttgatttcc ctgaatttct
    20821 gtctggtatt cttcaatcaa tgcaggcgtc ttttcttcga tctttttcga ataatcattt
    20881 acgattgctt gaagttgctc tggcaaacca tttgctgttt tttcttcaga agtttgtgtc
    20941 tcttttgttt tagttgatga ctcacttgtt tgtttcgtct ctgttttctt tgtcgttgtt
    21001 tccgttatgg tcgtactttt cgcttctgac gaattttttc catgaaaata tagacccgca
    21061 actcctcctg ctatgatcaa aaacacggca actgctgccc caatcatgat catccgctta
    21121 cgagctgcct gtttttgctg tcttcttcgg gtcatccgaa cattttctcc ccactcattt
    21181 tcatcataca tttttttcac caccaatttt tatgtataat agcgttatca aacgcttcat
    21241 attcttattg tagcaaataa ttgtcgctgt gcacattttt atctttgtta tttataagaa
    21301 aatttcacca gttgagatga ttccaagatt gtccgtcgat ttctccttgc agctgcattg
    21361 ttcgttcgtc tatctcagcc atcagtttct ttatttcata taagagcgca cggtcttgat
    21421 attctgattt ttcgcttaat tcatctaatt gttggatcag ccaatcattt gtcataatga
    21481 agttcctttc atttcttgca gtgtaaaggt caagtcttcg atctgctctt ttgcctcagt
    21541 cagtgcttct acgatatatg cttgtttttc agattcttct tcctgcattt tctgattttc
    21601 ctgtactctt tgaataaacc agtcaatccg ctgagtcagc gatggtttgc cttccaatga
    21661 gagatactgc tgataagcaa ttagcttctc ccaacgtttt tcctgggata aatacataaa
    21721 ttgtgctaaa acgaatggcg gaagataggt catatggaat ttatttgcga ttcgctgata
    21781 cggcctcaaa aattcggaaa tcgtatattc ttcttttcct ccagcttggt attctttttc
    21841 cgccacacct gttgtcaata cgattcctag ttctttccct tttaatacat gtgctgctcg
    21901 ttccaagaca ttatcttgcc aaatttttaa atgagcagga gaactatacc agtaaaacgg
    21961 aaactggaaa atgatccgat catgctgggc caagagctgt agttcagtct ctatatctat
    22021 ttttccgtct ggataacttg attccaaatg atgccaagta gctggtaaac cagcagaagc
    22081 ttctttgaaa aattgctgat ttaaagattc ttcgatcgtt ggatggctaa tgatgataag
    22141 tgtacgcatc ccttttcctc ctttttagtc ttcgctttac agtgtagcat aaccgccaca
    22201 agtttctttt gccgatgctt gtcattttaa ccagactaat caggttgtcc aagaaactgc
    22261 ggtttgtttt tccaactgtt ttttcaaggt atccaaagca atattcttaa aattttcgtt
    22321 tttcatacgt ttacgatagt tcgtagccgt ttctccggta ctctttttga acattcggct
    22381 aaatacgact agactattga accctacttg ataggcaata ctttccacgg attcatccgt
    22441 cagagaaaga agaaacgccc ctcgattgat ccgtaccccg atcaaatatt ccttgatact
    22501 ggtacccata tgatttttga atactcgcga caaatggctc cgattgaccg ctaaatgctg
    22561 ggcgatttcc tcaacagaca atttttcagc ataatgtgcc tcgatgtatt tgactgtttc
    22621 aacagctaaa gaggaatgaa ccttttgtac accaccgctg acctttccgc catcttttaa
    22681 aaggactgat aaaaaacggt aggttaatgc tgttaactgt aattcatttt ccaaggtttc
    22741 ttccgtatct ttcatgcatt ctaaaataat gtcgatatat ttttgtatgt cagaagagac
    22801 catcgtgtat tggtcttctc tgaaaagcga ctgccgaatg atttcttctg cagcactccc
    22861 accaaatgaa agccagcaat acatccacgg atcttctcca tctgataagt aaaacgtcga
    22921 gtctcctgga cgaatcaaga acagatcacc ttttttcaat tggaattgtt gatttttcac
    22981 atgataagat cctttgcctt ccatcacgac atgcaggatg taatcattgc gaatagcagg
    23041 accaaaagaa tgaaacggca tcgttttaga gaaaccacaa aaatcaaaat acaattcttt
    23101 ttctacttgt tgatgttcct tttttaaaaa aatcgattgc tccacgtcta tccctcctta
    23161 ctcctttagt ataatgcaac aaaaagttaa ataaaagtca cagaaatata ttaaaaagga
    23221 agcgatttct tgttaagttt aagtcattaa aagatctttg ttattcgttg taaccggttt
    23281 acttagtatt atatattgaa caggagagga atacagatta tgaaaagcaa aatgatccaa
    23341 cgtgtgtcct acgctttagg cgcttttgga cacgacacat tctatgttac attaagcact
    23401 tattttatgg tattcgtgac aagccagatg ttcgcaggaa cagataaggc aacgaatgca
    23461 aaaatgattg ctacagttac aagcttagtt gtaggtatcc gcttagttga aattattttt
    23521 gatcctttga ttggtggcat catcgataat actcgtacaa aatacggtaa attcaaacca
    23581 tggttagtta tcggcggcgt tgttagttcc gtactcttgg tagcaatctt taccaatttc
    23641 tttggcttag ctactcataa tacgacttta tttatcatat tatttgcaat cgttttcatc
    23701 ttattggata gtttttattc atttaaggat atcgcttttt ggtcaatgat tccagctttg
    23761 agtacggaaa caaaggaacg tgaaaaattt gctacgtttg ctcgtttcgg ctcttctcta
    23821 ggtgcaaatg gtactactct agtcgttgtc cctatcgtta ctttctttac ctatcttgtt
    23881 actcataaac atattgaagg cgcttcaggc tggttctggt tcggtgtgat tgctgctgtt
    23941 atttcaggcg gtactgcgat tatcactgcc ttgggcacaa aagaagtcga tactgcgatc
    24001 cgtcagcaaa atgaaaaagc tgggatcaaa gatgtgttca aagcagttgc tcaaaatgat
    24061 cagttgatgt ggcttgccct tgcttatatc gcttatgcga ttgccaatgt agcaacaact
    24121 gccgtattat tctacttctt caaatatgta cttggccaac caactgaatt ttggatcgtt
    24181 ggtctagttg ctgccttact tggtttagta gctgttccac tattcccaat gcttacacgc
    24241 gtcatctctc gtcgctacgt ttaccttgga gggatcgcaa tgatgatttc ttcttacttt
    24301 ttctttacga tcgcaggttc aagtttgcca atcgttatca tcgggctagt attgttctac
    24361 ttccctcaac aattgatttt cttatccgct ttgatgacga tcacagactc tgttgaatat
    24421 ggtcaatgga aaaatggtat ccgtaacgaa gctgttactc tatcaattcg ccctttacta
    24481 gataaaatcg ctggtgcttt gtctaacggg atcgtagggt ttgtcgctat cgcttctggg
    24541 atgactggta atgctactgc tgctgatatc actgctcatg gcgtaacgac tttcaaagct
    24601 tatgcattct atgctcctgc tgctttgatg attttctccg ctattatttt tgcttggaag
    24661 attacattga cagagaaaaa acatgcacaa atcgttgaga aattggaaaa caaattggct
    24721 ccagctgata caatggaaga tgaacttcac gatgcagtag ttccttccca ttaagaaagg
    24781 atcttgaaaa tgaagattac agaaaaagat tttggccaag gttatcactt gataaccttg
    24841 gagaacaaaa acaagctagc actttctatc tctgatttag gtgctagaat cgtcagttta
    24901 aaatcaaatg atcgagaatt agttttggga tttgacaccg ctgaagaata tatcgaaaaa
    24961 gatccgtata tcggtgcttc tatcggtcgc accgcaggga gaatcgaaaa tggccgcttt
    25021 tctctcaatg gaaaaactta ccaattagcc acagatccaa aaactggaca taatctgcat
    25081 ggtggtgctc ctggatttga attaaaaaaa tggtcttatg tcattttgaa tggcgaaaat
    25141 gaagcttctg tcatttttac tactacaagt cccgacggag aacatggttt tccaggtaca
    25201 atggatgtgg aaatccgcta tactttgaca aaagataata tctggcgagt gacctctcgc
    25261 ggcaccagtg accaagatac tttattcaat ccgacaaatc atgtttactt caacttgacg
    25321 ggagatgctt ctcagtcaat cgatcaacat gagctttggc tcaatagcga agcttatgct
    25381 cctttgcgaa cagatagtat tcctataggg gtaaaagaaa atgctgcagg aagtgctttc
    25441 gattttcaaa tacctaaaaa attagcgtcc gtttttgcta gtgatttgga tcaaaaaaat
    25501 cttgttgacg gaatcgatca tccatttttc ttaaaagaaa ccggcttggg taaagaagcc
    25561 gctcgcttga ctagccccga taaaagaatc caagtagata ttgctacaga tgcttctagt
    25621 gtggttattt tcactgcaaa tttcggcaca gaaacaccag aaatgcgcaa ccgaaaactg
    25681 gcacatcacg gtggcatcac atttgagact caaacagctc caggtgcaga gcgcttctca
    25741 gcatttggtt cgattcattt aaaagccgga tcagtatttg aaacagtaac agaattcaaa
    25801 atcaaaacaa gaaaggaata actcatcatg gaaatcaaaa gaagcttaaa cacaaaattt
    25861 gccgaaacat tcggacctaa aaaaactaca caatacttct caccaggacg catcaatttg
    25921 attggtgaac acacagatta caatggaggc catgtcttcc ccgcttccat cacttatgga
    25981 acgcaaggtg tagcagcacc tcgagacgat aataaaatat tagtttattc cacaaacttt
    26041 gacgatatcg gtgtcattag ttttacttta gatgagctag aatatgataa aaaagcaaac
    26101 tgggcaaatt acgtaaaagg aatggtcctt aaattaaaag aagctggaca ctcaatcgat
    26161 catgggtttg agttattagt ggaaggaaca atccctaatg gtgcaggact ttccagtagc
    26221 gcttcattag aattattagt aggtgttgta ttagaagatc tgtttgattt aaaaacagat
    26281 cgcttggaac tagtccaaac aggtaaaaaa gtagaaaatg aattcatcgg tgtcaattca
    26341 ggtatcatgg atcagtttgc catcggcttc ggtgagctag atcatgcaat cttattagat
    26401 acaaatacgc tgaaatacga aatggttcca gtgaaactag atggttatgc aatcgtcatc
    26461 atgaatacga ataagcggcg ggaattggca gattcaaaat acaatgaacg ccgtagcgaa
    26521 tgcgaagaag ccttggcacg tttgcaaaaa actttgccta tctcttcttt aggagactta
    26581 gatgaagaag aatttgaatc aactattgac caaatcggcg atgatacatt gatacgcaga
    26641 gctcgccatg ccgtttctga aaatcagcgg actttgaagg caaaagctga attagaagct
    26701 gggaacttag aagcatttgg tcaactgtta aatgattctc accactctct tcgctatgat
    26761 tatgaagtaa ctgggatcga attagataca ctagtagatg ctgctcaaaa acaagaaggt
    26821 gtcttaggtg cacgaatgac aggagctgga tttggtggct gtgcgattgc tcttgtcaaa
    26881 gaagaaaata tacctgcctt caaaaacaat gtctatgatg aatacatgaa agttgtcggc
    26941 tatgctccgg aattttatgt tgcacacatt ggaaatggga caacaaagat cgatccagaa
    27001 gctgttcatg tttgataaaa aaatcaacga tctcagctaa aaacaaatag ataaaaataa
    27061 cctctcagca atttccaaat gtgagccttt tgctcgtatt tgaaaattgc tgttttttat
    27121 tgacacggaa agagaacgtg tgttctaatt gtgttgaaga ggtgattgtt atgtgggcga
    27181 atcaaaatcc ggtatttgtt tatgaaaaag aaccttcaag agatatttta tgtattgact
    27241 gcaaatcttt ttacgcctcc gtagagtgcg ttgaaaagga tcttgatccc cttacaacta
    27301 aattagtagt catgtcctat ccgtccgatt cgacagaaac acggggtagc ggattaatct
    27361 tggcttccag tcctgcagca aaaaaagctt acggcatcac caatatcagc cgtgccagag
    27421 atcttccctt cccctatcca agtgatttat atattgttgc cccaaggatg gcctattata
    27481 tggaaaagaa cagagaaatc aattctatct ataaaaaata tgcagacgaa gcgaaccatc
    27541 atgtctattc cgtcgatgaa agcttccttg atgtaacaaa tagcatggca ctctttggta
    27601 aaacaaatgc gtacgacatg gctaaactga tccataaaga tgtgtttgag cagaccggaa
    27661 tctacacgac tataggaatt ggcgataacc cgctacttgc taaactagcc ttagacaatg
    27721 gctcaaaaga cggaaagcaa atgtttgctg aatggcgtta tgaagatgta ccagaaaagt
    27781 tatggaaaat ctcagatatc actgactttt ggggaatcgg caaacgaaca gcaaaacgtc
    27841 tgaaacaaat gggtatacat tctgtttatg atttagctca cgcggattat tatcacttga
    27901 aaagcaagct aggggtcatc ggtactcagc tttatgccca tgcttggggc atcgatcgaa
    27961 gttttttggg acaagaatat acaccaaaat caaaaagtat cggaaatagt caaatcctag
    28021 atagagatta tacaagacga gaagaaatcg agatcgttat caaagaaatg gccgaccaag
    28081 tcggcactcg cttgagacga tctgcagcaa aaacacaagt agtcagctta tgggtaggat
    28141 actctttagg ctatcttgat ccaaatggca agaccgggtt tcaccagcaa atgaaaatcg
    28201 aagcgacaaa tgctagcaga gatctagcag atgctttatt gaccattttc caccagcatt
    28261 accactacca ggatattcgg cgtgtaggcg tgaactgttc cgacctcgta tacgcgacag
    28321 gtttacagct taaccttttt gaagatcctg aaaagcagat aaatgaagcg aaagttgatt
    28381 ttgttgtcga tacgattaga aaaaaatacg gctttcaatc aatcatccat gcgcatagtc
    28441 tcttgcaagg aggacgcgcc atcgcacgta gttctctcgt tggcgggcat gcaggtggta
    28501 tgagtggatt agaaggtgga aaagatgaga cggacagcaa aaaaattcat tgactataac
    28561 gagtatcgag atcggccatt tggattaaaa tgggggactg cttttgcgat ggaagaacta
    28621 gtcaacggta tcaaaaaaat gaagaagaag cacgcaaaca gcctcttcct aaacaacaaa
    28681 tgacattgca agaaatcgat agtatactga cagaagcctt tttatctcgt aaagcagtaa
    28741 tcattcagct caatttaaaa gatgaatttg gaagactctt ggatgaggtc gaagggcact
    28801 tcattggtga agcctatgaa gattacttta tgattgacca tcagccgata ttgtgggaag
    28861 acgtacggta tattgagtta aaggaaacaa aaaaatggtt tgtgacatga gaggttgtga
    28921 cagaagtgat caacttcaaa aaataagaaa ggatttttcg aaaattgctt cttaaatttt
    28981 tgtggaattt tggcttattt ttcgaagaag caacttctgg aacaacgtat attagatatc
    29041 agaggttgtg aaaaagtcgc ttagttttga gtaacaagcc aaggcgctga tttcacaacc
    29101 tctgtctatc tatccatcta ttcggtaaga ttcatgcatg acgtcttctt tatttagtat
    29161 gtagagccct ttttcttctg accattcaac ataaatcagc tgttttacat ctgcatagcc
    29221 ttctttcttc aagttctcca taagccatgc ttcagtcaat tgattggact gaagcacttt
    29281 atgctgaatt tgcccttcat ctacaagcag gatcgataaa gctttgtctt catctgattt
    29341 tttcaataca ctcatttgac cggcattctc taggataaca tgtttggcat tttcaagaaa
    29401 aaagcattct tgctgtctta agattgctcg caactgctcc atctcgatat ggttatttgt
    29461 caactctgtc atgttgatga cgccgtcttt gattagaaca gatggctcgc ctttgaccca
    29521 acgattgacc ttttcatttt tttggacgat acgttcgata ccataaatca ttactgccca
    29581 aagcgcaatg gcaaatagga catgccctac atggacattg tcatcatagg ttgattcttc
    29641 taagatccca cctaaaacaa gcgtatagac taaatcaaac ggtgtgattt ctgacatgga
    29701 tttcttccca agcaaacgta ccactaataa taaaccgatc aacccgaaca ccagtttcat
    29761 tgtaattgaa aaaatcattt tttctccccc taactatctg ttaattaaat tatatatgct
    29821 ttctcacgaa cacgcatgga atatacaagg aagaaatgta tccttttact aaaaaaagga
    29881 ctgtgacact gtagtgaccc ccaaaagtta gacttttttg gagtggagaa atccactcct
    29941 tttttttatg ctctaaaatg atatttttac acaaccgtta cggttgtttt acgaaattgg
    30001 acg
//
LOCUS       DACDIG010000028        29399 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.028, whole genome shotgun sequence.
ACCESSION   DACDIG010000028 DACDIG010000000
VERSION     DACDIG010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.028"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            60..926
                     /locus_tag="IV247_001370"
     CDS             60..926
                     /locus_tag="IV247_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8777449.1"
                     /translation="MAIDGLAPGAYQLVETQAPTGYELDATPIEFEIERSQTAVVELT
                     KANRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPG
                     VYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVL
                     QGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFK
                     IEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLV
                     FISIKFRKKRNI"
     gene            complement(1350..1520)
                     /locus_tag="IV247_001371"
     CDS             complement(1350..1520)
                     /locus_tag="IV247_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8777450.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1714..1893)
                     /locus_tag="IV247_001372"
     CDS             complement(1714..1893)
                     /locus_tag="IV247_001372"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777451.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            2280..3392
                     /locus_tag="IV247_001373"
     CDS             2280..3392
                     /locus_tag="IV247_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8777452.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3470..3763)
                     /locus_tag="IV247_001374"
     CDS             complement(3470..3763)
                     /locus_tag="IV247_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAP8777453.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3931..4245
                     /locus_tag="IV247_001375"
     CDS             3931..4245
                     /locus_tag="IV247_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAP8777454.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4266..6245
                     /locus_tag="IV247_001376"
     CDS             4266..6245
                     /locus_tag="IV247_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP8777455.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6258..6584
                     /locus_tag="IV247_001377"
     CDS             6258..6584
                     /locus_tag="IV247_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAP8777456.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6600..7118
                     /locus_tag="IV247_001378"
     CDS             6600..7118
                     /locus_tag="IV247_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777457.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            7118..8470
                     /gene="celB"
                     /locus_tag="IV247_001379"
     CDS             7118..8470
                     /gene="celB"
                     /locus_tag="IV247_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP8777458.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8554..10059)
                     /locus_tag="IV247_001380"
     CDS             complement(8554..10059)
                     /locus_tag="IV247_001380"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAP8777459.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10151..10741)
                     /locus_tag="IV247_001381"
     CDS             complement(10151..10741)
                     /locus_tag="IV247_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8777460.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10920..12566
                     /locus_tag="IV247_001382"
     CDS             10920..12566
                     /locus_tag="IV247_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8777461.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12615..13334
                     /locus_tag="IV247_001383"
     CDS             12615..13334
                     /locus_tag="IV247_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP8777462.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13813..14760
                     /locus_tag="IV247_001384"
     CDS             13813..14760
                     /locus_tag="IV247_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8777463.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14772..15692
                     /locus_tag="IV247_001385"
     CDS             14772..15692
                     /locus_tag="IV247_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8777464.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15733..17190
                     /locus_tag="IV247_001386"
     CDS             15733..17190
                     /locus_tag="IV247_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8777465.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17535..18149
                     /locus_tag="IV247_001387"
     CDS             17535..18149
                     /locus_tag="IV247_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP8777466.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            18142..19866
                     /locus_tag="IV247_001388"
     CDS             18142..19866
                     /locus_tag="IV247_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8777467.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19863..21314
                     /locus_tag="IV247_001389"
     CDS             19863..21314
                     /locus_tag="IV247_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8777468.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(21787..23940)
                     /locus_tag="IV247_001390"
     CDS             complement(21787..23940)
                     /locus_tag="IV247_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP8777469.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(23942..24988)
                     /locus_tag="IV247_001391"
     CDS             complement(23942..24988)
                     /locus_tag="IV247_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8777470.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            25138..26427
                     /locus_tag="IV247_001392"
     CDS             25138..26427
                     /locus_tag="IV247_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAP8777471.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            26440..29142
                     /locus_tag="IV247_001393"
     CDS             26440..29142
                     /locus_tag="IV247_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP8777472.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            29293..>29399
                     /locus_tag="IV247_001394"
     CDS             29293..>29399
                     /locus_tag="IV247_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8777473.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSR"
BASE COUNT         9576 a         5089 c         5960 g         8774 t
ORIGIN      
        1 tacagaaccg agaaggagaa acgctacaaa ccggtctaac tacaggagaa gatggaaaac
       61 tggcaattga cggtttagca ccaggagcat atcagctggt agaaactcaa gcgccaacgg
      121 ggtatgaatt agatgcgaca ccgatcgagt ttgagatcga aagaagtcag acagcggtgg
      181 tagaattgac gaaagcaaac cgacttacac caggcggtgt cgtgttgaca aagatcgatg
      241 accaaagcgg ggaagtcctg caaggagctg tttttgagtt acaagatgcc aatggaaccg
      301 ttctgcagtc aggtctaacc acaggagcag atggaaaact ggcgattgac ggcttagcac
      361 caggagtgta tcagctggta gaaacccaag cgccaacggg gtatgaattg gatgcgacac
      421 caatcgagtt tgagatcgaa agaagtcaga cagcggtggt agaattgacg aaagaaaatc
      481 gacttacacc aggcggtgtc gtgttgacaa agatcgatga ccaaagcgga gaagtcctgc
      541 aaggagctgt ttttgagtta caagatgcca atggaaccgt tctgcagtca ggtctaacca
      601 caggagcaga tggaaaactg gcgatagatg gcttagcacc aggagcatat cagctggtag
      661 aaactcaagc gccaacgggg tatgaattgg atgcgacacc agtaactttc aagatcgaaa
      721 aagaacaaga agtggcgatc tttttaacaa aagagaatag aaaagttgct gatagctcag
      781 atataagagg aaacaatcca acaagcagtg gaaataagca tcttccaaaa actggagaaa
      841 ccatcattag ccagtttata ttgtcaatat tgggtgtgct acttgttttc atcagtataa
      901 aatttagaaa gaaacgaaat atatgagcta aaataaaaaa ttgaataagc atagttttta
      961 aaatcaacta gtacccatca attgaatgga aactaccgat tttagaaggg gtatacaaac
     1021 aaatattagt atcggagtag agaaggttga acccaaccag taaataagta aagacaattt
     1081 tgtgctgcgt tgagaagtga aaaataaacc tttgtagtaa tactagttaa taaaaaatat
     1141 acaaaaacac tgcgaaaatc attcttgagt ttcgcagtgt tttttgccta atgactcaag
     1201 tttattgggt aatactttga ttttctttct acaaataatc agcgatcagt tgtaattttt
     1261 tctctagttt taatggctct ttgtcaataa ggagaaaaac aagtcgggtc ttttgtctcg
     1321 gaaagcttca accaactcgt gaataaaggt tacgcctcct tgaaaggcgt ggaaaaacca
     1381 agtaatcgat caatcaccaa tttcgcattt ggtagaacat gtttggtgag ctgaaagtag
     1441 gtggcgttca tgtctgtaac taagaattct atttctttag gattagtgca gctaaggaaa
     1501 tagtttgtta atcgaggaat tttttgtgta ggtaaatttt ctcgattaat ttttctgtct
     1561 cgccatccgc ggaaataaaa ctcagtttat cctcaacgga aacgtgagaa cgaaattcac
     1621 aaccctcaac actcaagaca gaattctttt ggaagacttt ggtaaatagc ttctcaactc
     1681 tttcaatgtg cgaataacgg ttgtcaaaga tacctaacaa cttttcgaaa taaaaaataa
     1741 cgacactttt tcagtcagta aagaagtgat tttatgtctg acatgatttg aaatcgagtg
     1801 ccttggacgg ataaaataac tttgagcgtt ccaatggctt cgacagtttt tacaggtata
     1861 gcgctgcttt tttaggcgta taaccattgg catatgatta tattattcaa agtggacaat
     1921 ggtttccttc ttcttgtttt tcaccaccat taatttccta tttccatcta ccacagcaga
     1981 accacaattt ctacagacac atggagcagg gaagagaact gcagcaacga tcaatgtttt
     2041 tttcttctgt aaggtttcgt aagagacctc tgtaatcatc aaatttttgt ctattgatct
     2101 cagaattttt ttgatagaac cattcataaa gcatatcgtt ctctcagttg tttattttgt
     2161 ggtgatttaa tcatacaaga aaacgatatg tttttttata aaaacatgaa taggaaaaat
     2221 agttgacaag aaaatgacaa cgccatatga tgttccatag tatttacaac ctgtcataga
     2281 tgactgtaaa aaataaaagt aaaaagaaag gacgacaaca ggtggatttt tatcagtctc
     2341 tacaactaga tccgtttatt ttaaaacaaa aaatacgaga agcagcttct aaaaaagaaa
     2401 agtgcatgta tatttgttca ttgtttttac gatcattatt catcgtattg tttgccattt
     2461 gttttatcat tataattaca acattatttg aatcgaagca taaaccatac gctgtggttt
     2521 tattctgtat gttgatgagt attcgttttg ttgactttgg ttataagatt tcccattcga
     2581 tcataggttt agcaatcgtc atgttctcac tattagtagc accgtatgtc cagctgatca
     2641 aatggtcagt tatgggcatg ttgattcatt ttattttact aagcagcata ttaatggcaa
     2701 ccgctagtga tcccaaaatg gggaatgctt ctttgtacgg cttttcatat ctctttattg
     2761 tttattcgtt acctaaagat tcgttgaaca agaatttctt tacacaaaca agtagcttat
     2821 tatttttgtt tttttgttgg ttttcagttc tcctttacag aaaacatcga gaaaagaaca
     2881 aagacaaaag cttgtttaaa gagatttttt tgaaaggcat gtattcgcag gaaaaaattt
     2941 ggatgctcat ctatgcattt ggcattagtc ttttgatcgt tgcaggagag tatgtccctt
     3001 ttcagcgatt gatgtgggca gggtttgcat tttcttctat tgtatctagc tatggattga
     3061 tgtcgattgg tttcaaagaa cgagcagtag accgaatcat tggctcattg attggatgtg
     3121 ttttatttat tggtatcagc caatttattc cttttaactg ggtcggtata ttaggtggtc
     3181 tagctttagg tgtttgttct acctatagat ataaaacagt ttttaattct tttggtgctt
     3241 tggcaatcac tgccagttta ttcggggttc caggagcagt aacgatccgc atttttgaga
     3301 atatccttgg ggtatgttta ggaatcatgt atattggggt gactgaaata cttatccgaa
     3361 aaataagaga aaaacacggt ttgaatcatt agatgagatc gaataaaaaa taaggaaaaa
     3421 gtgaagatac acatggatga taaattgcta tcattccatg tgtatctttt tacggtaaat
     3481 cagtgaaacg ttcgactttc atttttaatt tatacttttt acggagttcg tttatttgag
     3541 ccatcatttc ggatttgtga tgcttttcga ttgcttcttc atctttccaa cggtcgatca
     3601 gtaaaagaga ttctggatct tctagtggaa agtaatagtc atatttctcg tttccttctt
     3661 ctgcacgaat cgctttgaca atgtttcttt cgaccatttc ttctgcaaat tttctggctt
     3721 gtccatttgt acttgtatac aaaatattca ctgtgatact cactaaatcc cctcctgaaa
     3781 aaattctatc actagtatat cacggtgcca gaaattatta ttcaagatat gatacacatt
     3841 ttttccgcaa tgctacggaa atatatattt tgaatagaaa acgcttacaa gatatgcttg
     3901 gtttataaaa ataaaaggag tgattgattt atgaaaaaag cattgattat ttgtgcggca
     3961 ggaatgtcat cctcgatgat ggcctcaaag acaacggatt tctttaagca aaaaagagaa
     4021 gaggtactcg tagatgcagt ttcagctaca gaaggcgata atatgattaa gaatagtgat
     4081 tttgatttat ttctgatcag cccgcaaacg acgatgtttt tggataaatt caaaaaatta
     4141 ggagaatctg ttggaaagcc tgttgttagt atccctttcc aagcgtatgt cccaatccct
     4201 acaggcatcc agaagttagc agaggtgatc gaagagaaca tatgatggaa taggagtgga
     4261 tgataatgaa acagaaggaa aaattgctgt tgagatattt gattgatcac aagaaggagt
     4321 atgtcaccag tcaacgttta gcttctgaat tgttattatc tgatcggaca atccgtaatt
     4381 acttgcagcg gatcaaagag ttagtagaaa aaaacggagg taagatcatt gctaaaccag
     4441 gttatggcta ccaactgcat attttacaaa gactgacatt tgacttgttt ttgagtcggc
     4501 aagaaatagt acctggatat gctagagaac cgcaaggatt ttatgagtcc gaagatcgac
     4561 aaaagtatat attgaaccaa ttgcttttgg aagatgccat tctttttatg gatgatttgg
     4621 cagaagagct ttatatcagt cgttccagtt tgacaaagga catgcaagaa atcaaggagc
     4681 gattgattcc atattcacta aaaatcgttt ctaagcatgg tcaagggaca tggatcgaag
     4741 gggaagaaag aaatcgacgg cattttatca tggatacttt ttttggaaac cattatggaa
     4801 attcattgaa agaatattta gggcaaagcc gttttttccc agacatcagt tttgaagagc
     4861 tagtcattat cattctcgat gaaacaagag aagctaagtt aaaagtttcg gattttatca
     4921 tacagaatct gactttacac ttggctttgg ggatcaaacg gctccgagaa gggtttgaaa
     4981 tcaaggaatt aggcatcgaa caagaagttt ctaagcgagt agagtatcaa gtggcgcagc
     5041 ggatcgttca acgtattgag gtaatcgcag gtgtatcgtt tcccgaagaa gaaattgctt
     5101 atttgacatt acatctcatg gctaagtcga atcacaaaga aaatcaaaat gatcaggaac
     5161 tatcaaagga attagcgaaa gttctgaaaa gattgtctca ggaatttcat ttttcattag
     5221 tagaggatct ccagcttttc aatggactac tcgatcattt gtcaccgatg atgattcgat
     5281 tgagccgagg tattcgcatt gagaacccat tgactgagga gataaagaaa gaaaatccca
     5341 aagtctttga cgcagtaaaa aggcatttca gcaacatgcc ggctttgaaa aactatacca
     5401 tcaatgaaga tgaatgggcc tatttggctt tgcatctcat ggcggcattg gaaaaagaaa
     5461 gagctgcgca taaactacat gcattgatta tctgtgcaac gggttacggc agtgcccaac
     5521 tattaaagaa tcgcgtagtc agtgaattcg gtaaaaatat tacagtggta agtgtaaaag
     5581 gctattatga gatcaatgaa acaactttaa aaggaatcga cctgattatt tcttctattg
     5641 atctttcgac aatgtttttc aaaattccag ttttacatgt cagtattttt ttgaatgaag
     5701 atgatgttcg taaaatacga aaggtagttg gagaatctat accctatcat tcctcagata
     5761 gaccgatacc agcattttct ctacagcaaa aaaagcagat ttacaccgaa cagttgtctg
     5821 aaaaattttt caagacttat acgtacgaac cgaaaaagga agaaattctt catgatttgc
     5881 tgcaaaagct gagcataaat gaagaagaag gatatataag agaaatgcga aagcagatcc
     5941 agcaacgtga aaatatggga caaatcatat tcagtgatac agtggcagtc ccacatcccg
     6001 ctgttccagt tggaatcgaa acaaaggtag ccgttgcatt gattccttct gggatgcagt
     6061 gggatcaata tcaagaaatt cattttgttt ttttagtttc gccttcttat gtcgaaaatg
     6121 aaggaattac ggtcgtgact aaagcaatcg ttaggttagt tgaccagcta aaagtacagc
     6181 aggaaatctt agctgaacct atttttgaga atttcagcag taaatttatc aaactgatcg
     6241 aataaggagg aaaacagatg gctgaaaaaa taaacagtga aacactacaa gtgacttctt
     6301 ttgatatcat tttccatagc ggaaatgcac ggacactgat acatgaagca tttcgctcga
     6361 tgcgtaaaga gatgtttgaa gaagtagaag agaagttgcg acaagcaaat gaagagattc
     6421 ttcatgcgca tcagtcacaa actcaacttc tgaaagaata tgccagcggg acaaaaatag
     6481 aaatggaaat tatcatggtc catgcacaag atcatctgat gacgacgatg acattattgg
     6541 aagttgcaca ggaaatgaat tatttgtatc aaaaaaataa atagaaagga aggatcattg
     6601 tgcaggatac agtaaaaagg aaaatcgaat tagaaaaaaa gcaacttagt ttgaaaaaca
     6661 tgtatttcaa tcgttatcta atcgtacgtt acctgactgc ctttttcttt ttcatgaaca
     6721 tgcaatggat gattctgctt ctatcagcta aaagtttagg aagtagttta ccgatgattc
     6781 tgattttagc tattttgcct gcagtaggag aacaagtaaa gctttatcga aaacaccaaa
     6841 cgaacgttcc atggacaaag tggtattttt tgttccagag tgtctttaat ttattagtga
     6901 ttccagtgtt gtttacctca ggatttacat tcttgtatcc ttttatggca aataacaatg
     6961 gaggacagtt atttgttttc attcttgttg taagcggtat ttttgccagt gcatttatac
     7021 agtatcgttt aaagaaaatt tcactcaacc aagaccaaca gtatatccgt attaaacaat
     7081 atgaaaaagc gttatattta ggaaaggaga ataactaatg tttgattttt tgcagaaata
     7141 cctgatggga cctatgggga aaattgcgca acttaaaatc gttcgggctg tcatggcagc
     7201 ggggatggct tctattcctt tcacgattgt aggctcgatg tttttggttt taaatatttt
     7261 gccattaccg ttcccatttt tagaaggatt ttttaatgca acttttttca aagttagtga
     7321 cttgtacatg atcgtcaata caatgacgat ggggatttta tctatttact ttgccattgt
     7381 gtttgcttat gaattaacgt caattgaacg agatgaacaa aaattaaatg ttaatccgct
     7441 cacgggggca ttactatctg tttttgcatt ctttatgtgt atccctgaat tgatcatgac
     7501 tgatggtaaa atcagcttga ttgcttctat caatgagaca gaaactgtag taaatggtat
     7561 taggatgggc tcttttgtcg aacgtttagg aacttcagga atctttacag ctattattat
     7621 gtcgattatt gcggtcgaat tgtattgtat gtgtgtcaag agaaattggg tcataaaaat
     7681 gccagatgtt gttccgccag gagtttctcg ttctttcact gctctgatcc caacatttgt
     7741 cattgcgttt gtggtgatga tcatcaatgg attattagtt gcactaggaa cggatatctt
     7801 caaaatgatt gctatcccat ttgggtttgt tacgcagttg acgaatacat ggcttggtgt
     7861 catggtgatt tatttcttga tccatgcatt gtggatcgta gggatccatg gagcgaatat
     7921 catcacttct ttcttgaccc cgatcgtatt agctaatatg gcagctaacg cacaaggcgc
     7981 aaattatccg ttggcaggag aattcaacaa ttcttatgta accgttggtg gatcaggtgc
     8041 gactttagga ttgatcatct ttattgcatt cctcgcgaaa tctgatcagc tgaaagtgtt
     8101 aggaaaagcc tcacttgtac caggattatt caatatcaat gaaccaatca ttttcgggat
     8161 gccaatcgtc tacaatccct atttagcggt tccgtttttc ttagctccga tggcttctgc
     8221 tacactcgct tattttgcca ttcggctgga aatcgtcaaa ccgatgcttg cacagatgcc
     8281 gtggccttca ccagtcggga tcggagcatt tgttggtagt ggtggtgatt ggaaagctgc
     8341 tgtgttagca gtcatttgtg caatcatcgc attcattatc tggttcccat ttatcaaatt
     8401 ctatgataat aaattattaa atgaagaaag agaaaaggca gcagaattag ctagtgaaaa
     8461 gttaaaatga tttctaagaa aaaaggaact gtgacaaaaa atcacagtcc ctttttttct
     8521 tagaaatggg aaaccactag tatataaata ccattattct tcaatcaacc gcttcacgat
     8581 tgcataatcc ccttcataag gttcatctac accattgatt ttttgatagg gatccacgcc
     8641 ttttcccgca ataacgactg aatcttgcgg agcgctttct gctaaagcgc gtcgaatcgc
     8701 ttcaggacga tccatttcat aatgaacgat caaatctgga ttagtaattg cttcattgat
     8761 ttcttccgcg atttttttcg gatcttcaaa tgctgggtca tctgccgtca aaaaggcgat
     8821 gtcagctgtt tctgatagca cttttccaaa atcatgtcga cgggagattg ctttgtttcc
     8881 tgggctgcct aatacaacaa ttacacggcc atctggatgt tcatttttgg caaatttcag
     8941 caatgttttc aagcttagat aattatgtgc atagtccaca tagacatgcg ctccattgga
     9001 ttggatcaat tgatccattc ggcctggtac acgagcttct tttaatccgt cttgtgcatc
     9061 ttgactggag gcacctgtta gtgctgcggc aatgatcgca ctggctgcat tttcttggtt
     9121 gaatcctcca gccagcaaaa tatcataagc agtatcgcag atagctaagc gatcttcatg
     9181 agaagataaa gtaaaaccgt gagtaccttt atctgaacgg atcacttgat aattgctttc
     9241 ttccgaacga ccataagtga tcgttggaat ctggaataga tcggctgttt ccctaagtaa
     9301 atcaaaatag tcactttcat gatttaaaac aactgttttt gaattcaaga tcaattgtcg
     9361 cttgcaatag aaatagtcat caaatgtcgg atgttcgatc ggactgatat ggtcaggcga
     9421 gatattcaag aagattccga catctaaagt caagccgtat acgcgttgtg ttttgtaagc
     9481 ttgtgaagaa acttccatca ctagatgagt cataccattt tcaactgctt ctgacatcat
     9541 acgatataaa tctaatgact caggcgttgt caaatgggat ttgaaatatg ttttgccatc
     9601 taacgttgta ttcatagtag aaagcaatgc tgttttttta tttgttgtgt gatccaagat
     9661 tgcttttgta aagtacgcag cagttgtttt accttttgtt ccagtgaatc cgatcacttt
     9721 caatttattt tgcggatagt cgtaaaaagc catagcaaga agcgccattg ctttacggat
     9781 atccgttacg atgattccta ctgctgaatt ttcgtatggt tgttctgata cataatattg
     9841 aataccctgt tcgattgctg aatccaaata ttcttctttg aaattcattc ctttacagaa
     9901 aaacaaagta gaagcatcag cttttcttga atcataactt aactgaagaa attcagcttt
     9961 ttctggtaca tcaagatgcc agcctcttgt tgaaacaaat tcttttaata gattttcttt
    10021 taatagtaaa tgacggatgt cttctaaaga gaagctcatt gattgatccc tcgctttatt
    10081 caatttcttg gtttattata gcacactctt tttaatatat cgtttgtttc cgctatgaca
    10141 aaatccagct ttattcaaat tttcttgaca caggggacaa gcggatgagt attttcataa
    10201 acaatgaact gactgtaata gataggagaa cgaatagtgt ctccaacaat ccaaaaacat
    10261 aataattcaa aaagacgata agactattgg taagtaaaag tccttgcggc attggaagcc
    10321 gaaaaaattt gtggaaaata atcggcggta tcgttatacc gccatttgag gccccgactg
    10381 tgtacaatgt gtaaacccca agactgaaca aaagacttcc tgaaatcagt gaaaagaaat
    10441 gtgaggagat catttccact tttggaacaa tctctaaaac aacaggaaaa agcagacttc
    10501 ccgctacagt cttaaaaaat actgattttc ctaataggac agcggctaga agcagcatca
    10561 taagattgat gctccaaaca gtaactgagc gttggataga aaaggccgtt tcaactaaat
    10621 gtccgatggc gcccactccg gcggaagcta tatcgtgagg gagcaagaac caattcaatc
    10681 caactgtgat aaagaaaaga cctaaaaata taacgggtag tttttttatt tcttgtgtca
    10741 ttgatatctc ctataaatgt tagccataaa aggctgtata tttctttact ttaccaaaaa
    10801 aacgaaaccg aattcaaaaa aacaattgtt cttataaaag ttatataaaa tcgattgcca
    10861 gtttctgttt taccaattaa aatgatagta atgttttgta gaaagttgga atctgaccga
    10921 tggataatca aagaaaacca gcattagatc aattaacacc gcaagaaaaa atggcacgag
    10981 gctctgcttg gatgaccgcc agtaatatca tttcaagatt gctaggcgct gtctatatca
    11041 ttccctggta tgcttggatg ggcgaaaatg ctaaggctgc caatggatta ttcaatatgg
    11101 gatacaatat atatgcattg tttctactga tctctacagc aggaatacct gctgcgattg
    11161 caaaacaaac tgcacgctat aattcattga atgaatatgg cacttcacga cgattattta
    11221 tccgtgcatt acagatgatg ggcgggttag gcctgttgtt tgctttgttc atgtacatcg
    11281 cctctccttg gctagctcat gcttctggtg gcggagaaga actgattcca acgatgcgtt
    11341 ctttgagtat cgctgtattg gtttttcctt gtatgagtgt gatacgaggg tatttgcaag
    11401 gcaatcaaga aatgatgcct tatgcgctgt cgcagattgt agaacaggtg gcacgtattt
    11461 tttatatgct attggctaca ttcatcatca tgaaagtcat ggatggtgac tatgtgacgg
    11521 cggtcacgca atcaactttc gcggcattta tcggtatgct agcaagtatt cttgtccttt
    11581 tgtatttttt gaagaaacat caagcgtata catcggcgtt tattcactac agcgaaaaca
    11641 aaataaatat tgcaacgaag gaattgttgt tagataccgt taaagaagcg attccgttta
    11701 ttattgtggg ttcaggggta acgatcttca aattagtcga tcagttcact tttatgaaga
    11761 tcatgtctgc atccaccgag tattccaatg cacagttact ggatcttttt tcgattttta
    11821 gtgccaatcc agataagttg acgatggtag ttattgcatt ggcaacatcg atcgcgtcta
    11881 ctgggttacc gttgattaca gaagctgtta cgttgaaaga tcgagtaggt ctggcaaaat
    11941 tgacaagcgg aaatttacag ttgttttcat tttttatgtt tccggcgaca tttggtgtta
    12001 tgttacttgc ctatccgcta aatacattat tttatacacc ggattctcta ggaagtaatg
    12061 tactgatcca agcaagtttt gtaggactct tcctaggact atatatgcta gtttccaaca
    12121 tgctgcaagg aatgtttgaa aacaaggcgg caattaatta tttggttatt gggtttctag
    12181 tgaaactcct tcttcaatac ccagccattc gtttatttga agtatacggc cctttattgg
    12241 cgacaatgat cggatttgct gtgtcttgta ccttgatctt aaagagaatc catgaagtag
    12301 cccgctttaa accaagtttt gtctggcgca ggacattgct gatctttatt cttaccttga
    12361 ttatgctttt ggcttcgtgg atcacaaaga tgatttttgg tgctttttta aacgaagata
    12421 gcaaattcca gtctctattg ttgattcttg tagtagcagg tataggggct ttagtgtatg
    12481 gttatttagc tttgaaaatc cgcttggcag aaagactact aggaccagga atagcgcgtt
    12541 tacgacataa attaaaaata aaataagtga atcagaagag ctagattgat gctcttttga
    12601 aatgggggaa acgcatgcgt ttagataagt ttttagcaga tatgggactt ggcagccgca
    12661 aagaagtcaa aggattaatc aaaaaaggaa cgatcgaagt caatggacaa gtggtgaaaa
    12721 gcgataaagc gcaagtaaat gaattcgaag atgcggttac ttatttagga gaaatcatta
    12781 cgtatcaaaa agatttttac tacattttgc ataaacctgc aggagtcatt tctgctactc
    12841 aggataatca tgatcaaacc gtaatcgatt tattggaaga ccaggattat cgggaagatc
    12901 tttttccagt cggccgatta gataaagaca cagagggcct cttgatttta acgaatgacg
    12961 gggtgtttgc ccatcagtta ctatcaccta aaaaacatgt ggagaaagaa tatctagctg
    13021 aggttagcgg tataatgaca gaagaagata tccgtttatt tgcagaagga ctgattattg
    13081 atggcgatga gcagacattg ccggcaaaac tccaaattga tcaagtagat caagagacag
    13141 aaacttcaat tatccgtctg attttgcatg aaggaaagtt tcaccaagtt aagcgaatgg
    13201 tcaaagcagt aggaaaagaa gtcttatatt taaaaagaat ccgtatggga aaattttggc
    13261 tgcctaaaga tttgaataaa gggcaatatc gacccatgac caaagaagag ctagaacaag
    13321 tgagagaaaa ataattgaat ggatagctaa aagaataagc agagtagttg tagcgccatc
    13381 tgaaaagcag acttgtccgt ctgactttcg gatggctttt ttgtgaaatt tcggcttatt
    13441 tccgtagaag ctacttctgg aacaccgttt gttcggaata agggactgtg acaagacttt
    13501 tgttacagtc cccattgttc cttgtgactc agagttagat agtaaggaca cgatctccct
    13561 aatactcagg ccaaaacgct gaattcacaa cctctgataa acggtgttca atcagctgac
    13621 cctcggtatt agctcaaaaa acggcaaaat atgaggaact attttcccgt ttttttctct
    13681 aatactcagg ccaaaacgct gatttcacaa cctctcctat actttgtttc attttcccta
    13741 tactttgttt atttattctc attattgaaa gcgctataat tacgttgcga atgaatcgaa
    13801 aggggttttt agatgaaaaa acgaaagggg ttcaatcatt tttttatgaa tctatggcgt
    13861 tataaagcac ttgtcctgat ggctgtaccg ggtatgcttt ggatgatttt tttcttttac
    13921 atacctgtaa tggcgaatgt cgtagcattc aaaaattttc atatttcagc agatgggttc
    13981 ttggctagct tgagggaaag tccttgggtg ggttgggaaa atttcaaatt tttattttct
    14041 tcggatcaag catttctcat cactaaaaat acaattttat ataatgtagc atttatcctt
    14101 ttgaatctgc tgatctcagt cgtatttgca atcattatga gcgagttgag aaataaacga
    14161 ctcgttaaag tttatcaaac gatgtcctta ctaccttatt ttctatcttg ggtcattatc
    14221 agttactttg tttacgcttt tttaagtcct gacaaaggaa tattcaatca gtggatcact
    14281 ggacatggag gagaagctgt caattggtat aatgaaccga aatattggcc gtttatcttg
    14341 gtgttcattg gtacatggaa agggattggt tataacagca ttatttactt tgcatctgtt
    14401 atgggaatcg atccgactta ttatgaagct gctatggttg atggagctag caaatggcag
    14461 cagatcaaac atgtgacgat accacaactt attccactaa tgacgatttt aacgatttta
    14521 gccgtaggaa acattttccg agctgatttt ggcctgtttt ataacattcc taggaattcc
    14581 ggtgcattat atgatgtgac tcaagttttg gatacgtata tctacaatgg cctgacatcc
    14641 acaggagatt ttggtatgac ggctgctgca ggactttacc aatcagttgt cggctttgtc
    14701 ttgttgatga tcacaaatac gatcgcccga cgttttgata gcgattctgc attattctaa
    14761 ggaggaaaaa aatgaaaaaa aagaaagtgg agagagtcca agtccgctct ttcaataaga
    14821 cggctaattt tttctctaat ataatgattg cactctttgc tatttcctgt attttgccat
    14881 ttatatttgt tatcgccatc tcttttaccg atgagtccat attgtcgact cacgggtaca
    14941 gtttttggcc agaaaagttc agtatatttg gttatacgta cttgttcgac cagatgcagg
    15001 ataaaatttt ccaagcgctt ttcgtcactg tacttgttac tgttttaggt acactgatca
    15061 atagcacggc tacttcttta tatgcttatg cgatttcacg gtcaaacttt ccattccgtc
    15121 gtttttttac cgttttttgt ttgattacga tgttgttctc accagggatg gtggccaatt
    15181 atcttgttat gacaaatctt cttcagttaa aagatacgat ttgggcattg atcttgccaa
    15241 tggctgtcag tcccttcaat attattgtga tgcgcacatt ctttaaacgt tcggtctctg
    15301 attcgattat tgaatcggca cggatcgatg gagctagcga actgcgaata tttgttcaga
    15361 tcgttttacc gttagctgtt ccaggtattg cgacaatcag tttatttgcc gcattgggtt
    15421 actggaatga ctggttcaat gctttactat acatccaaga tgataagcta gttccactac
    15481 agtatctttt gatgaaaatc caaagtaata ttcaatatct gacacaaaat tcaggagcag
    15541 gaagccaatt gacaggcgga ttagcctcag ttccaggaga atctgccaga atggcgatcg
    15601 tagtcatctc gacgttgcct attgcgatca gctatccatt tttccagaag cattttgtca
    15661 aaggtctaac aatcggcgga gtaaaagaat aaagaaacaa aaatataaat cataaaatta
    15721 ggaggaagaa aaatgaagtt atggaagaaa ctagccttta caggtgtttc agcgttgttg
    15781 ttgggaacat tagcagcatg cggaggttct ggttctaaag accaggcaga agctagcaat
    15841 tcggacactt tgcagatgta tcaaatcgga gataaaccag ataatttcga tcaattgatg
    15901 gaagtagcaa acaaacgaat cgaaaaagag atcggtgtaa aagtcaatat caattacatt
    15961 ggctggggcg attacgaaaa gaaaatgaac gtcatcattt cttccgggga aaattatgat
    16021 atcgcctttg cgaacaacta tgtttccaat gcccaaaaag gagcctttgc tgatttgact
    16081 gaattagcgc ctaagtacgc tgaaaaagct tataatgatt tagatgaagc atatattaaa
    16141 ggaaatctgg tcaatggaaa attatatgct ttcccagtca atggaaatgt ctttgctcaa
    16201 caagtactta cttttaacaa agctttacta gataaatacg atctttcaat tgatggaatc
    16261 caatcttacg ctgatgcaga aaaagtcttg aaggaattcc atgaaaaaga accaaacacg
    16321 gcagcttttg ccattggaca aggcttcaaa gtccaaggga actttgatta tcctttaggg
    16381 aatacgttac catttgctat tgatctggat ggagatgatt cgaaaatcat caaccaatac
    16441 gataatgaaa cgtatatcgg actgttacgt acaatgcata gctggtacaa acaaggactc
    16501 attccaagtg acgctgcaac gagcaataag gattatccac tagaaggaaa tacttggcta
    16561 atgcgtggag aaacacaagg accgtatgat tatggcgata caattttgac aaatgcagca
    16621 aaacaagaac ttgtctcaaa agcaattact gttccactaa aatctacagc tcaagcacaa
    16681 atggcaaatt ttgtcgtatc aaatacttca aaaaataaag aaaaatcagt agaacttcta
    16741 gggctgctta acagtgatcc agaattattg aatgggcttg tttgggggat cgagggagaa
    16801 gcatgggaaa aagtcgatgg ttcagatgat aaaatcaaat tgctagatgg ttatcagcca
    16861 aatacacata tgtctgcttg gaatacagga aacaacaaga ttctatatac acaagagtct
    16921 attacggatg acatgattgc caaacgtgat caatcgattg ctgatgcaga aacttcaccg
    16981 attttaggat tcagcttcaa cactgacagt gtcaaaacag aattaagtaa tatttctaat
    17041 gtcatgaatc aatatttaga tggattgaat acagggactg ttgatccaga tgaaacatta
    17101 ccgaaattga aagacgcttt ggacaaggct ggctatgata aggtattgaa agaaatgcaa
    17161 aaacaatatg atgagttccg acaagaataa taatgaataa agacaaaggg gttgtgacaa
    17221 aagacgtgtc acaacctctt gttccgaata aacggtgttc aaaagccagt ttctcggtat
    17281 taagctaaat atatgtaaaa atgtggaaag ccatattcac aaaatttttc ttactactcg
    17341 aaactaagcg gctatttcac aaactcttgg taaacggtgt tcaatcagct aaccctcggt
    17401 attcgctcac aaaacgacaa aatatgagga actatttttc cgtttgtttc tctaataatc
    17461 aggtcaaggt gctgattgcc caaccttttt ttgtataatg agaaagtata aacttaatgt
    17521 ttggaggaga agatatgtta gggaaggttt tggaagagtt atttatccgg atctgggtca
    17581 ttataaaact aaccttgtat ttctggatat atacatttgc cggcgggatc atctttggat
    17641 taggtgcggc atggaaaaca gtcaatgaac tattttattt gtacggattc gagtataaag
    17701 aaatcacaat aaaaagaggc tggaatatat ataaaagaaa ttttttaaga ggcaatctgc
    17761 tatttagttt atttttatct ggtactgctt tattgagtta caatttatat ttatctgttc
    17821 aaatcaaagg gctcattttt ttagctattg attttatttt actttttgcc ttgctctgcc
    17881 tatttgctac ttatcaatac agtatgattt tagatagtga atatacgatc tctgttccta
    17941 atctgctaaa gctaagtttt atttcggttt tttccagctt ttcgtctttt ctaaaaataa
    18001 ttattggtag cggtataatt ttaggcgtga cttggcaatt caaaggattg atcctgtttg
    18061 gtgtcattgg tcttttgacg gtttggaatg gtacgatgac aacacattgg cgtgaagaat
    18121 tagataaaca gttggaaagt tatgaataag tttcggattc tattttcaaa aggaacctta
    18181 ctaaatcgtc tgttgcggaa atacgcggtt atcatgatcg gtgtaacgat gactgctacc
    18241 gttattttta gtatccatac ttgggaacaa aatcaaaaac aggcagaaaa catgacaagt
    18301 gatgccgttc aaagcacaag cagaatgctg aatgataaaa caaccttgag taggatcatc
    18361 aaaaaccagc ttgtagggaa ttccgaaaag atcgaaaatg tgacgaccta cttaacaaaa
    18421 cccatagatc agtatctgat gtatgtatac gaacagcaaa acagtaccga tgaattagtt
    18481 tctttcccaa atcaaatcaa agatctctac gcaaattatg aagaactaag tgcgatatat
    18541 atcgtgttga atcaactacc ggagtattat gaatcttcta gagaaaacaa aggtggggaa
    18601 ataaaaaagg ggacaccaaa attactagac gccttttata tcaagttgcc tatcacacaa
    18661 ggaggagctg agtcaggaag tatttttatt ggttttcaaa agaaagaact agatatgatt
    18721 ttagaaaatc taacgtcatt caatggactc tctttataca tgatttctgg aacgacaaat
    18781 cgactataca cattccatga taataaaata gctaaagaaa cgtttgaaag acaagaaatg
    18841 atcatcacag agagtctgaa gaaaaattca caattgccga ttgacgatct ggaaaaaaac
    18901 aattttgttc aacaccaaga actgtctgat gattatcgta tattagctgt actagataaa
    18961 aaaagtgtaa aaatcgaaac gggaaaaaat cttgccccgc tgatcattgg tatcattatt
    19021 ttagacaata tactgcttat ctttctttat aaaacattca aacgatattc acagcaagta
    19081 tctatcgtca tgcaggccat ggatcaaaca gcagcaggga atttagagac gcggatcgac
    19141 ataacaaaca cggaatatga actgaaggaa ctttcaatcg gtatcaacga aatgttggat
    19201 agcatcaatc agtttgtaga agacatctac aaattagaaa tcaaacagca agacgcacat
    19261 atgcgggcgc ttcaagcaca gatcaatcca cactttttat ataatacatt agaatatatc
    19321 cgaatgtatg caatcagcga aggaagtgaa gaattagcag atgttgtgta tgcgttttcc
    19381 gctttgcttc gaaacaatac gaatcaagag aaaacgatca ctttaaaaga agaactggac
    19441 ttttgcgaaa aatatgtcta tttgtatcaa atgcgttatc ctaatcgagt agcctatcat
    19501 tttatgatcg atcctgattt ggaaaagatc gaagtaccta aatttgtgat ccagccatta
    19561 gttgaaaatt attttaaaca cggtattgat tttactcgat tcgataatgc gctaagtgtc
    19621 aaagtcttgc aggaaggcaa acgggtaagg atcatcatca aagataatgg aaaagggatg
    19681 actgaaaaac ggctaaaaca gattgaagaa aaactgtctc atcctaaagt ggaacttcac
    19741 ggttctatcg gtctgcaaaa cgtgaatgaa cgtttacgtg ccagcttcgg cagcagttat
    19801 tatatgtcgc ttgagaataa tgagacaggt ggactgaccg tttctattac attcaaggag
    19861 ggatgagcgt gtatcaagtg ttattggtag atgatgaata catgattttg aatgggttaa
    19921 aaaagataat tgattggcaa agtttaggct ttcaaatagt agcaacagca gagaatgcca
    19981 aagaaggact agctgtactt gaacagcgac agattgattt agtagtaacg gatgtgacaa
    20041 tgccagaaat aaatggactg gaatttattg aagcggctca aaaagaacgt cataattttg
    20101 aatttatgat tttatcagga taccaagaat tcgattattt gaaaggaggt atgcaattag
    20161 gcgccgtgaa ttatttgatg aaacccgtga ataagttcga attggttgaa agcctcaaga
    20221 aaatcaaaac acggttagat cagcaaaatg aacaaaaaaa tcaacaagaa atctatcaag
    20281 aactgttatt cagtcaatgg ttgaatgaag aattggatga aataagtgaa gaagaaataa
    20341 taggacaaat aagtgagaag cagcatagga tagtattgat tcaactggca agaactcatg
    20401 atactcttat caatagatgg ttgcaagaac ggcaacaaat cttttactat caacgaaatt
    20461 acggagaccg tgtattgatc acgttgttgt tggaggcaga tgaagttgat ggcttctgtt
    20521 gttttgttca agaaaacttt cctgatcagg aatggctgat cagtatcggg gaagagacag
    20581 attctttgga caaaatccct gaaacattcc aacaagcgaa agacaatcta cagttgcatc
    20641 aattttatgg agataaagac gagcatgttt tttacgcagt acaagcagaa acaaaagatc
    20701 aaaccatcga tttttcaagt ttcagacgcg tgctgcaaaa taaacaattg gaagtcgctc
    20761 aaaaaatgat cactgatttt tttgaacagt ttcagttagc agccatgatg ccggaagata
    20821 tacgatattc cagtttttta ctgtttatgg agattcaacg ggaattgatt gatctggaag
    20881 atgaggaata tttacaagga atcgaaaaaa tccatcaggc aaaaacggtg caagaacttc
    20941 agcagctact tctgtcattc atcaaaaagc atcagagaca gaaaaagtac agtgacaatg
    21001 tggaaaaagt aatcgtaatc ctgcaccagc attatcaaga accacttaca ctcaaagaag
    21061 tgtcagaaaa cctgcattta aatgtcatgt atctaggaca gctgttcaaa aaagaaacga
    21121 aaaaaagttt ttcagcttat ttgaaccatt tacgtatgga aaaagcgaaa caactgcttt
    21181 tgcacagtaa tcaaaacatc aatgagatag caagtgagat tggctacaac aacaccacgt
    21241 atttctctaa attattcaag aaaatcgtcg gcagatcccc taaagagtac cgagaaaaca
    21301 gtggagaatc ataaaagaaa aagccaatgt aatgaaataa cttataaaaa attgtctaga
    21361 aatctaattt tgggctgttc ttattacttg aggcaaggca gctttttcat gacctctcga
    21421 taaacggagt tcaaaagcca gtttctcggt attaagccaa atttatgcaa aaatgtggaa
    21481 agccattttc acaaaatttt tcttaatact cgaaactaaa cagctttttc acgacctctc
    21541 cactaaacgg tgttcaatca gctgaccctc ggtattagct caaaaaatgg caaaatatga
    21601 ggaactattt tgccatttgt ctctctaata ctcaggtcag aacgctgatt tcacaacctc
    21661 tcaataaacg gtgttcaaaa gcttgcatct gcggtaataa gaccaaccaa acgaaaaata
    21721 tgaggatcta tttttgcttg gctgttctta ttacttgatg caggacagct ttttcacaac
    21781 ctcttgttaa ttatagtttg ataaggaaaa aggcaaatct tttgcttgat atttcttagg
    21841 gtgagggatg atccctaatt ggtaagagat cgtgccgcct ttcagcaaat cttgatgggt
    21901 aaaatataat ttcgtatatt ctttgtccat ccgtttgatt ttatggataa attgttgctg
    21961 cggccgatta ggcgttgctt caatcgtcaa ttcctttcca tttgacaaaa gcaaaacagc
    22021 cttgtcaatt aatggcatac cgataacata ttcaccggtt ccaggcgtga caggataaaa
    22081 tcccagacta ttgaagatat accaagcggc catacttcca ttgtcctcat ctccagggta
    22141 acccattgga gaatcgtcga aggtctgagt catcaactgt ttaagcagtg gctgtgccat
    22201 ttcaggttgt cctatataac tgaacaagta aggatagtga aaacttggct ggtttgaaat
    22261 agctaattgt ccaaattcta tcgcggccat ctcactcatt tcgtggatct caaaaccata
    22321 tcctcctggg tcaaacgttg ggggctgatt gcacagctca atgagttttt ctttatatct
    22381 ctcactgcca ccatattttc cgatcagtcc tgaaaaatcg tgataaacag caaagctgct
    22441 ttgccaagca cttccttctg cataatcttg tccccaacga ttagctgaaa atggttctct
    22501 gaacttgcca tctttatctt ttgaacgcat aaatcctgac tcatagtcaa agatattttg
    22561 atagttcaat gattgctgct gatatttctt cgtcatttcc tcttcttcta gtaccttggc
    22621 aacttggcta atacaaaaat cgctataagc atagtctaat gtgtgattga ctgattcgtg
    22681 gtagtgatta ggaacatagc catatgtcaa gtaatcttct gttccttggc gtccataatt
    22741 tggatgtttg ctttgaaccg tagcaccttt ttccattgcc gttagaaatt caggcatcaa
    22801 gtctcgtcgg atatctttta cagcagcatc tgcaatgacc gcatcgatca atgtcccagg
    22861 cataagtcca cgttcatcag gagacagcca ttttggcaaa tagccacttt ctctatagct
    22921 gttcaaaaaa ccttccagca tttcttcgta tttttctaca gcaatcaaag aataaagagg
    22981 ataaaccgtt ttatacgtat cccaaaaccc attatttgta tagagaacac caggttttat
    23041 agatcgacta gtcgtatcat agtggatttt ttgcccacta ggatcaattt cataaaatgt
    23101 ttgtggaaat aaaaacaccc gatataaatt atgataaaac gtccgcaatt gttgttgatc
    23161 atggtgtgtg agctgtatac gatcaaaata ggactgccat tgttcttgac tgtgagcaag
    23221 gtaagtctct ttgctttggt tttgttctag ttccaggtta taagctgctt gttccgagct
    23281 gataaaagat gtcccaaatc ggatagtttg tttttttgtc tgttcaaaat acaacgggat
    23341 cgagtcgcta tttttgttgg aaacagctaa ctcatcaatt gttaaaggat attcaaatct
    23401 caatataaaa taaaaagaaa aatcaggatc ttctgaacca gcaaaatgtg tgatttttcc
    23461 ttccactgtg taaggatcta taacaaagag ctgatggcgt cccggaaagg ataaaagtaa
    23521 accgctgtca tttttgctgt aatcaataga taagattccg ccatacatgc taggtatcaa
    23581 tgtagaacag atgccataac gtaattgaca gatcgacaga tgagtcgggc aaaatatact
    23641 ttcttctggg cggtaagaac tttgagcatg gaaaagggtg ttctcctgta gttttccatt
    23701 gatcggcgta aacagcatat gactgaaatc acccatccaa ggacttggtt gatgggtaag
    23761 acggtagcct tgaaaaacgc ggtcatcagg atgaaaccac cagctgccgt tttgatccgt
    23821 agtttgcggt gcaaaataat tcatcccaaa aggaacgcca gtataaggct ggcagttccc
    23881 attagagaaa tttggctgat tcgctgttgc atgtctagta tctatttgac cgatattcat
    23941 tttatttctc ctttgttgat tgtcttttga tgaatgaaac tggaaattta ctatcggatg
    24001 gtcgatcttt tgtctctaac caagtgacca aagcatctcc cgcaagctta cctaactgtt
    24061 caaaatcttg tgcgatggtt gtcagtggcg ggtcaacaag agctgctgct tggatatcat
    24121 caaatccgat aattgaaaaa tcttcaggga tatgataacc atgctgtctc aagagattca
    24181 tggtctggat agcgacaaca tcgttttcac aaacaaaagc agttacttca tacttagtga
    24241 tataagaaag caattcatct aatgtcgcgc ttttgtcatc cagtgaagtg tggaaagtca
    24301 aaccagcttt gtccaatgct tcaaggtaac caaggtaacg ctgtcgaacg gattgtggat
    24361 gagtctgcgt accaaacaaa taagcgattt ttgaatgtcc ctgttcgatc aaataagagg
    24421 cagcaagcgc accgccttgg aaattatcgg aaagaacggt aggaaaaggt aactcataga
    24481 tttttttatc taatatgacg acagggaatt ctttcaatga aagctcaaaa agtaagtcaa
    24541 gatgttgttc tgtatcaaga gcataataaa tcaatccgtc aaattcattg attagctctt
    24601 ttgctgtttt attttgtaga aagtcaagcg tcgtcatcat gacatcaatc tgtttatctt
    24661 gcatcacagg gttcaatccc ttgctaaaat caccaacaga caaatcagcg acatatggaa
    24721 gaagaaacag aatccgtcgt gtttttgtag gagaagcaag aatctgttcc ggagaagcgg
    24781 atttaacgaa actgcctttc ccgcgaacgc gataaataag tcctaattgc tctaattcag
    24841 tcaatgcacg tttggaagtg attctgctta ctttataggt acgtgaaagt tctttttcag
    24901 ttggtagttg gctgtctaca ggtaattcac cagttaaaat agccgtttgc aaatcattaa
    24961 atattgtctg gtacaatggt tgattcatat aaacacctct taatgatata tcatattcaa
    25021 ataataccat aatggatgaa aaagtaaata aaaagatatt ttttgaaagc gtagacataa
    25081 ttggctgtat gaagtataat ctttaaagat atatcaaata gaaagaaggt tttattagtg
    25141 gcttataaag atataccaaa atcggtgcag aaatttatgg aagatatcac acaaaaatgt
    25201 ggggatatcc atgcggactg ggcaaagaat tttacagcag catttgccaa tactctgttg
    25261 acaacagtca aaaagcaaaa tgacggaacc acctttttat tgacgggaga cattccagcg
    25321 atgtggttaa gagactctac tgcacaagtt cgtccttatt tagtgattgc aaaagaagat
    25381 gaggaattag ctgacatgat cagcgggtta gtcaaaaagc aattttttta tatcaatatc
    25441 gatccatatg cgaatgcctt caacgaagaa gctaacggtg ctggccacca aacagatcat
    25501 acaaaaatga acgattggat ctgggaaaga aaatatgaaa ttgattcttt atgctaccca
    25561 atacagttgg cctatcttct ttacaaaaat acaggaaaaa ctgatcaatt caatcaatct
    25621 tttattgaag gtatcaaaaa aatcttggac gtgttcaaaa cagagcaaga tcatgaacac
    25681 tctccttata catttgtacg agacacgaca agaaaagaag atacgctgat caatgacggg
    25741 cgaggtgcaa aaacagcttt tacaggaatg acatggtctg gtttccgtcc tagcgatgat
    25801 acttgccgtt atggatatct ggtaccatcc aatatgtttg cggtggtcgt gttaggttat
    25861 gtggaagaaa tttttcaaac gatactcgat gataaagaaa ttgtacagca aacacagaaa
    25921 ttgaagaaag agatccaaga ggggattcag aatcacggac gaacggttaa tcaaaaaggg
    25981 caaacgattt atgcatatga agtagatgga atgggaaacg ccacagtcat ggatgacagt
    26041 aatgtgccca atctgatcgc cgcaccttac ttgggatatt gttctgaaca agaggaagat
    26101 tatctgcaaa caagaagaac attgttgagt aaagaaaatc catatttcta tgaaggaaag
    26161 tacgcaaaag gaattggatc ctcccatacg ccagaaaaat acgtttggcc gattgcatta
    26221 gctatggaag gaatgacaac gtcagacaaa tctgaaaaag agcggatatt gaatttactt
    26281 gtagcaactg acgcaggaac acaccttatg cacgaaggat tcgatgtgaa caatcctgaa
    26341 aattatacga gagaatggtt ttcttgggca aatatgatgt tttgcgaatt agtcatggat
    26401 tactttgata ttagagtaga aaagtaggag gtatcgtaaa tgaaaaagaa agtatatatc
    26461 atttctcata gccactggga tcgtgaatgg tacatggctt acgaacagca tcacatgcga
    26521 ctgattgaat tgatagacga tctactagag ctatttgaag ttgatccgtc cttcaacagt
    26581 tttcatttgg atgggcaaac gattatttta gatgactatc ttcaagtaag gccagaaaaa
    26641 agacaggcaa tccaacaagc aatcaatgaa ggaaaattga gaattggacc attttatatc
    26701 ttgcaagatg attttcttat cagctcggaa tcgaacgtga gaaatatgct gattggcatg
    26761 gaagaaagcc gtaaatgggg aacccctgtc atgttaggct attttcccga tacatttgga
    26821 aatatgggac agacgcctca attaatgaaa caagctggta tttcagcagc agcgtttggc
    26881 cgtggtgtaa aaccaattgg ctttgataat caagttttgg aagcagaaaa ctattcttcc
    26941 caatactcag agatgtggtg gaaaggacct gatcaaacag caatctttgg tctgctattt
    27001 gccaattggt atagcaacgg gaatgagatt ccagtagaaa aagaagcagc tcttgccttt
    27061 tggaagcaaa aattagcgga tgcagaacaa tatgcctcta cgaaccactt attgatgatg
    27121 aatggtgttg atcatcaacc ggtacaaaaa gatatatcaa aagcaatcca tctcgcaaat
    27181 gaactgtttc cagattacga gtttattcat tctaatttta ctgactatct ggaagcagtt
    27241 caaaaagatg tgccagaaga cttaggctca gtagaaggag aattgacaag tcaagaaacg
    27301 gatggctggt atactttagc caatacagca tctgctcggg tttacttaaa acaatggaat
    27361 accaaggtcc agcgacagct agaaaatatt acagaaccac ttgcgacgat ggcttatgaa
    27421 gttagtggaa actatcccca tgatcaatta gattatgctt ggaagacatt aatgcaaaat
    27481 catcctcacg acagtatttg cggttgttca gtcgattctg tccatcggga aatgatacct
    27541 cgttttgaaa aagcagatga agttggaaag tatcttgcac aagattcctt agaacagctg
    27601 accgcagcaa tcgatacgac cggattccca aaagacagct ttccatttgt cattgtaaat
    27661 actgcaggaa tggataaaac tggtgaagca gaaataacca tcgaattaga aagaaaaaga
    27721 ttcgctgaag gaatacctga acaactatat caagagttag aaaatttgcc taaacgtaag
    27781 tatcatgtag aaacaaaaag tggggccact ataccagcta ttttatctga agaaactgtc
    27841 caatttggtt atgatttgcc gaaagaccgt ttccgtgtgc catatatggc aagaatgatc
    27901 aaagtgactt taccattaga aaacatgcca gcattttctt gggaaacatt cgcgcttgta
    27961 gaaggggaag cgaaagcaga agaaaaggaa acaatgattc atcagagtgg acgaatcatc
    28021 gaaaacggcc ctctacattt gacgattgaa aaaaatggta ccattacaat ggaagatcgc
    28081 aaaaacaaac gaaagttgaa taatctgcat atttttgaag atatcggcga tataggaaat
    28141 gagtatatct ttaaacaacc tttctgtgat cagccaatcc tatccagtaa caaagaaaac
    28201 tctgaagtaa aagtattagt agatacaccg gaaatagcaa aaataagtat tctacaggaa
    28261 atggaaatcc ctgtttctgc agacgagcgt ttagaaaaag aacagcagat ggttgtggaa
    28321 tttcgttatc gaaaagcaga acgatccaaa gaaaaacgaa tccttcaaat aaaaacaatc
    28381 atgacaatca gaaaagatag taaaaaaatc gactttgaaa cgactctcga caatcagatg
    28441 aaagatcatc gcttacgtgt cttgtttcct acaaagttac atgtggaaca tcacgaagca
    28501 gatagtattt ttgaagtggt gaagcgccca aaccatgtgt ctaaaagttg ggaaaacccc
    28561 acgaatccgc aacatcagca agcatttgtc aatattcatg atgaagagta cggtgtaaca
    28621 gtcggaaact ttggtttgaa tgaatatgaa gtaacagaag acggacaaat cgcagtgacg
    28681 ctacttcgca gtgtgggaga actaggcgat tggggatatt ttccaacacc agaagcacag
    28741 tgtctaggtg agcatcggtt caattacagc attgaactac atggaccaga agaaaaattc
    28801 tctacttatt tgcatgcata cgcagcacag attccttttt ccactcaaca aatcaaacat
    28861 catgaaggaa cattgatttc aaaacaacaa tatttaacga taaaaagtga aacgtttgca
    28921 attacagctt tgaaacgcag caagtttagt gataaagtag tagtaagagg tttcaatatg
    28981 tctagtcatt tagaaaaatt ggaaataact aaagacaatg gaaaaactgt catattgaat
    29041 ctgcttgaag aaccaacgaa gcaagcggtt gtcccaataa tccaacctta tgagattcgg
    29101 acaatcggat tcgaggagga aaacgaatgt atggcggaat agaagcaggc ggaacaaaat
    29161 ttgtgtgtgc ggtttcagat ggtggagaaa ttttagaaac gccgattgta aaattaagct
    29221 agacaactaa aaaagtcatt tgtgctacac tcaaaatagt tccgaccaaa gaattataag
    29281 gagtgagtac aaatgaccta tacccatctt acaacggatg aacttgtaat aatagagtct
    29341 tatttcaaaa tgaatcaatc tgttgctaaa actgcccatt gcttgaatcg ttcaagaca
//
LOCUS       DACDIG010000029        28068 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.029, whole genome shotgun sequence.
ACCESSION   DACDIG010000029 DACDIG010000000
VERSION     DACDIG010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.029"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(89..667)
                     /locus_tag="IV247_001395"
     CDS             complement(89..667)
                     /locus_tag="IV247_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8777474.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(687..1355)
                     /locus_tag="IV247_001396"
     CDS             complement(687..1355)
                     /locus_tag="IV247_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8777475.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(1370..2440)
                     /locus_tag="IV247_001397"
     CDS             complement(1370..2440)
                     /locus_tag="IV247_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8777476.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            complement(2611..3975)
                     /locus_tag="IV247_001398"
     CDS             complement(2611..3975)
                     /locus_tag="IV247_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8777477.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(3972..5531)
                     /locus_tag="IV247_001399"
     CDS             complement(3972..5531)
                     /locus_tag="IV247_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8777478.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(5587..8067)
                     /locus_tag="IV247_001400"
     CDS             complement(5587..8067)
                     /locus_tag="IV247_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777479.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(8051..8386)
                     /locus_tag="IV247_001401"
     CDS             complement(8051..8386)
                     /locus_tag="IV247_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777480.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(8400..9434)
                     /locus_tag="IV247_001402"
     CDS             complement(8400..9434)
                     /locus_tag="IV247_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP8777481.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(9524..10204)
                     /locus_tag="IV247_001403"
     CDS             complement(9524..10204)
                     /locus_tag="IV247_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP8777482.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(10374..11333)
                     /locus_tag="IV247_001404"
     CDS             complement(10374..11333)
                     /locus_tag="IV247_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8777483.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
     gene            11577..13028
                     /locus_tag="IV247_001405"
     CDS             11577..13028
                     /locus_tag="IV247_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP8777484.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            13377..14009
                     /locus_tag="IV247_001406"
     CDS             13377..14009
                     /locus_tag="IV247_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAP8777485.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLTGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            14597..16036
                     /locus_tag="IV247_001407"
     CDS             14597..16036
                     /locus_tag="IV247_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8777486.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            16331..16549
                     /locus_tag="IV247_001408"
                     /pseudo
     CDS             16331..16549
                     /locus_tag="IV247_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            16798..17013
                     /locus_tag="IV247_001409"
     CDS             16798..17013
                     /locus_tag="IV247_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777487.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(17230..17486)
                     /locus_tag="IV247_001410"
                     /pseudo
     CDS             complement(17230..17486)
                     /locus_tag="IV247_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(17673..18584)
                     /locus_tag="IV247_001411"
     CDS             complement(17673..18584)
                     /locus_tag="IV247_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8777488.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(18603..19607)
                     /locus_tag="IV247_001412"
     CDS             complement(18603..19607)
                     /locus_tag="IV247_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP8777489.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(19604..21727)
                     /locus_tag="IV247_001413"
     CDS             complement(19604..21727)
                     /locus_tag="IV247_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8777490.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(21732..24179)
                     /locus_tag="IV247_001414"
     CDS             complement(21732..24179)
                     /locus_tag="IV247_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8777491.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(24166..24555)
                     /locus_tag="IV247_001415"
     CDS             complement(24166..24555)
                     /locus_tag="IV247_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8777492.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(24615..25118)
                     /locus_tag="IV247_001416"
     CDS             complement(24615..25118)
                     /locus_tag="IV247_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP8777493.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(25131..25355)
                     /locus_tag="IV247_001417"
     CDS             complement(25131..25355)
                     /locus_tag="IV247_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777494.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(25459..27369)
                     /gene="ltrA"
                     /locus_tag="IV247_001418"
     CDS             complement(25459..27369)
                     /gene="ltrA"
                     /locus_tag="IV247_001418"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP8777495.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         8246 a         5600 c         4960 g         9262 t
ORIGIN      
        1 aaaaactacc tatataaaga agcaagatat aacgatcttc gtcatatctt gcttcttcac
       61 ctaactattt tctccctata taacagcttt acgcttctcc taatacacca ttcaaatata
      121 aatcgatcaa ttgcgccaca tttagcggtt cttccacagg tcttttcgac cgagtgatgt
      181 aaggtgctaa cactaagaaa aaatgccttg ctgcgatttc ttttgaaatc ccatttctta
      241 gcacagattg gtttgctgaa aagatttctt caaaaggttc acgataattt ttcttccata
      301 gaaagaaaag atgatctctt gtttcctttg aaagattgct ttcaagatcg tgaagcatca
      361 gaaacaaatc gaccagatgt gtctcaatca aaaactgcgc catttttgtc aaggttactt
      421 gaaatcccgt ttcgcttgtt tgaacgattt ctctcaaagc ttttccaaca tcttttaagt
      481 ggttttctaa aactgccgta tatagttttt ctttatcacc gaaataatga tagagattgg
      541 gctgggtgat cccaactttt tgtgcgattt ctcttgttga agtgccgaga tatcccttgg
      601 acataaacaa gtcagtcgct tctttcaaaa ttttttgctg tgtatctttg ttgtatcgat
      661 ggaccatttt tccacctgct ttttggttat tctttttctt tcaatacacc atcttccatc
      721 ataaatattc tatcgcattt gtcaatcagc cgtgtatcgt gagtaaccat gatcgttgct
      781 ttatttttgt cttttgattc ttttgccaag atatcgacta cttctcgtgc cttttctgaa
      841 tccaaactag cagttggttc atctgctaga ataatggacg gatcattgta caatgcacga
      901 gcgattgcga ccctttgccg ttctccacca gatagttctt ctggatactt gttgatcaac
      961 ttatccacgc caagctgctc aaataattcg tttgcttctt tttgacgatg ttcatgttta
     1021 atcttatcta ctaataataa ctgctttttt actgtcagaa aaggaaccag attggaagct
     1081 tgcaaaataa agccaatttc tttaaaacgt agtttaacgc gttcgctttc tttttgatta
     1141 ctgaattctt gatcattgat aaatacagag ccactgctag gcgtctgcaa tccgcctgcc
     1201 aaagtcaaaa aagtactttt acctgaacca gaaggaccga tgattgcaac aaattcccct
     1261 ttatcgatac tcatggttgt ttctttcaat gctttaacta cttggtcgcc atcttggaaa
     1321 cttttttcta cttctttaaa ttctattgct ttcatgttct tcctccttct tatccaattg
     1381 ccttcaacgg atcgattttt acgattgtcc gaacagaaaa tagtgcgcct aacacagcta
     1441 ctactaacat caacaagcta atgacgccaa aaaacagcca attgctttgg aatggaaccg
     1501 catcaggtag aacaagggat gttcccactg ttcctaatag accaagaaga ataccaacaa
     1561 atgataaaat gaatgtttgt gccactacag aacgcgcaat aaatcctcct gtgatccctt
     1621 gtgctttcat tacaccaaag atattaatct tttgcatcgt cagtacataa atgaaaatcc
     1681 cgatcacaat agccgcaata acgatcaaaa atccgatcat aaatccaaaa gtcaatactt
     1741 gcgcattata tccaggtaat tcattgatga atttagaaat tttgatctgt tctaaatctt
     1801 caggtaaatt actgatttta cctctgacaa ctattgcgtt aatccgtgca ttttcacttg
     1861 tatcttcttt ttcgaagcgg atttcttggt aagcattgat ggttgtatag agaactggtg
     1921 agacattgaa tttggcatga tccgtaaagc cagttagttt aaacgtcttg tcactaccag
     1981 ataacttaac tgtatctcct atggctagac catattcttc tttcaaacta atatcgccta
     2041 ctgcctcatc atcgttatca aaagcttttc cttcaacaag attgggcatg atgaattgat
     2101 ttttatcgat accgaagaag ctaacattga tttttccttc ttctgtactg tctttactag
     2161 tcacgactcc aggtgtttgg gcaagatacg ccactttccc accttcgact tcttctgcta
     2221 tcttcttagt aatcatggac atccctagat ttgaatttgc atctttcgaa agcacaatcg
     2281 aatctgcctc ccacttgtcg actgctgttc gattatcttg agctaagcca taagctaacc
     2341 cagtcaaaaa aaataccagg taggcgatca aaaacatcac ccctgcaact aacgcataac
     2401 gtaactttga atgcataatt tcatttaatg ctaaaaacat ttaactcctc ctcctgaaat
     2461 aatcatttta tacaacaatt ttatcacttg ataaattttt ggcgaatatt atgctcaaaa
     2521 aattcttccg ctatttcatt atatgaattt tttattctcg attgattaat atttattata
     2581 tccccacaca ataaaagacc gtgatatttc tcacagtctt ttatctactc tattgctatt
     2641 tgctctctta gctagtactt taaaagctaa ctcattgagg tagtgaaaac gagaggtgta
     2701 ttcagacaga aatccaaaat cctcaactgc tagctgtgga atcgtagttt tgtgtttcac
     2761 gatatccgca agcaggttca aatctgataa taagaattct tggcttatca gctgtccgcc
     2821 aattatttct aatgtatccg gatggtagat cagctttgca tttactgttt tgttttcttc
     2881 gtcgtattgt ggaaatagat caaattctcc tgaacacgaa ccggtatttt gttccaagaa
     2941 tggcgcttca tcttctgtca gccctacagt tcctaaaaag tagtctataa tgactgctgc
     3001 tgttatcttt tgtctgaggt cgtaagcggt tggctgacca ctcaaagtta gcgctactgc
     3061 tcttgccata tgtacagctc gagtaactaa aggtaaaaag agatgccgtt tatttttgtc
     3121 aaaactgact gggacgatat ctccaatcgc atagatgtcc ggatcacttg tctgcagata
     3181 ttcattgact atcacagtct catcctcata caaagccact ttttctttta acaatgtcgt
     3241 attaggacga gtttgggcag aaaaaatgac catatctgcc tttattgttt gagacagaag
     3301 ttgtacggat actactttct cttctgattt tttccaatcg atcaagaatt catttggata
     3361 aaaatttatt tgcttagttt gcagctgatt ttgaaaactg ttgatcatat ccttatcgaa
     3421 atatcttgat aaaacattcg gcatataatc aactagatga ataatgtatc ctttatttct
     3481 caaagcatct gctaattcca atccaatgta accagcaccg aagatcacta cttcttttgc
     3541 ttcttttaaa gcggcaagta cttgcttaga ttgcactaga tttttatagg tgtagacatt
     3601 ttcagcttca atttgtttat acaaggtgac aggagaggaa cctgtcgcta cgattaattt
     3661 atcataggtt atggtctcgt tttttccttc tttggatgcg aggatgagct gatgtttgtc
     3721 tggatcaagt tcagttactc tccattcggt gatcagtttt gccccacttt cttgaagagt
     3781 ccgcatgtta tgggcaactt cagctaaact acgtatcttt ccttttaaat aaaggttgat
     3841 tccatttgaa acatagccta gtccatcttt atgatcttca tcgatcagta aaacttctgc
     3901 tttcggttga agttttctta gatttagggc agctgtgatg ccttcatggg aagcaccaat
     3961 gatcacgatt ttcatgtttt attcatcctt aaattcaatt tttctaaata acattctttt
     4021 aaaaattggt tcaaataggg gaaatcataa ttatattcgt tgctagtgaa tacaaaaact
     4081 ttagcggact tctgtgacgg gtactctttc aattggctag tcaaaatgat atcatactgc
     4141 ttttctttct cgtattcgac taaatcatat ttgatcgtat tcagcaattg ttcctggatg
     4201 atttctttca cgatatccgc cattagcctt tcataaggaa gatcataagc aatttgcaga
     4261 cgtaattcta actgcttgat cagcgtccgt aaaatagatg catagcgagc aaataattca
     4321 aagcgggcat tttcctcgtc tacttcagta aaatcaacaa tagcaaatgt ttgtctgacc
     4381 aatctttcac ataaaaccag gaattgttta tcttctactt cagttaaccg ctgttttaat
     4441 ccttcttggc caaaaatatt catccatcct ctaaaatagg caaaatgagc gtgattttgc
     4501 atcactgtca acgcaatctt ttgcgaaatc tcttctggaa aaacataaaa agagaaatat
     4561 tcttctatcg attgtttaaa cgttcgattc aattgagcta ctacgtcgtc tttgtatttt
     4621 tcgcttatca aaaactcctg gacatgcgag ttatgtgtat ctaatgtaaa gttactgatc
     4681 atgaaaagat aaaaaaccaa cgtttcattt tgattccaag gaaaggcata ttgaaagaag
     4741 taacgggtca aaaatgtatg gaggtccaat aaaaattgtg tcgtttgaaa caatccaaca
     4801 ggaagattgc tataatctgg tttggatttt ttcgtacagc gcattcgctt cttcataatg
     4861 gaaagccata tttcaaattt caatcgtcct tcttctgtaa tagcaaaact aaactctttt
     4921 tgcagcagat ctaaaaaagt gtttcctgat aaatcactgt attctttttt gttttcaaga
     4981 tagggacgtc caaaccaaaa taattggaaa taaaaataac gaatttgact ttctggaccg
     5041 gtcagccgcc cgctttttat tttcagatga aactcattta aagaatcatt taattccttc
     5101 actctgcgag acaaagtagc cgaactgata ttaagttcca tgacaagttt ctgccgatca
     5161 aatgagccat tttccaacag ataaagaagt atctgatatt ttgtagaata gcgcaagtaa
     5221 caatgataat aaacatcgac tgctgctata tttccatgtc tgattaataa aatattccct
     5281 tcttggataa caatttccaa ttgtttgata ttttccaagt tagcttgtaa ataaaacaga
     5341 tcttctttca aggtagcccg atcaactgat aagcgggtaa gtacgttatt aacggaaacc
     5401 tctccttttt cctctaataa aagttggaaa agtttgattt gccgttgttc atttttttca
     5461 agcaaactga aaagttccaa gtccactcct tcaagcattt ctgattttaa cagcagaagt
     5521 ttccttctca tccttgaaaa agaggatctg tgacagttat cacagatcca tacaaattct
     5581 aatcacttag ctggttggcc cagctgtcag ttcccatgtg accatgcctt gatacgttgc
     5641 gccaggttga gcattcgtac tgctatttac ctctaagagt aaaccatcgg tatcgggaaa
     5701 gtcaacgaga acctctcctt tagctgttcc atcactctca taaatcaacg tgttttgacc
     5761 ttgctgaatc aattgctttg atccagaatg attgaagtat aattttgttc cgcttaattc
     5821 ttgaccttga tcattcttaa ataaagaagt caatgtacct gtcagttgcc aaggagacgt
     5881 gcttggaagc cgagtatccc gcacatcaaa agaaaaatcg ccttttgatt cataataact
     5941 tgtcttcaac ggtttgggga gatcgttacc aaatgtaaaa tcatctggta cattaactaa
     6001 tgttaattct tgtttgggga caagaattgt tttagcagat gtttggacaa ctgggttagg
     6061 aagcttagca ccatctgcta atgtaatatt gctggaaagt acagctgcct ttgtcagtat
     6121 gctgacggat tgtaaagaag tatctacagt atttaattga tagttgtaag taagatgaat
     6181 ggtatctcct attttgctca atagatctgc tgggacagaa actccttgtt gaatgccggt
     6241 atctgcgata gttgtaccat ttaattttaa ggaaccattg atataagtag tattttcagg
     6301 aagtgagatc gtgatttttt tgtcggttat cgcatcccca gattttgtta acgtgaatgt
     6361 cacattttct tgaatcgtat ctcctgatag agcagacagg ggatctcctg tctcaggttg
     6421 tctgctaagg ttttttagct gactatctgc actcacagat ggaaaattag ccactgttat
     6481 ttgaggaaga gtggtgattc cagcttgttc agactgccat ttgtacgcaa aagacggata
     6541 catgttagta agtacatcgt ccaccaagcg ataatcaaaa taatattgcc ctgaagccaa
     6601 tgcaggtagt gtagaagtag ctgtatttac acccccgtta tttgattgga tcgtttggaa
     6661 aagttgatct gtccatcctg tcgctaattt ttgggttcct ttcgaataaa gcctgtaatg
     6721 gatcgaaccc ttttttagtt cagttgctga ccaattggcc gtgacttggt acgatgcaga
     6781 agaaaggagc tttccccact gactatcggc tgttaagttt gttggtaaaa ccgttccgtc
     6841 tagcttgtac tgctccaata cctttaaagc aacaggagaa agcaatccgc cttgcgcatc
     6901 tttcccactg tacacgatat tggctgtctg attcaatcca cttgtgcttg ctggagtatg
     6961 agtcagtatt gctccttgtg taacgttttt tcctgtaaaa tagttgacta cgcgatcaaa
     7021 gcgactaaat ggcaactgat caaaacgtcc ttcagtcagc cccatcgtca atgtccgact
     7081 attggtagag cctggaaggt tctggttatt tccatccacg tattctgtgg tatcagcaaa
     7141 tgtattatat tttcctgttg aatcacttgg tgagatgtaa ctattccacg tatttatctc
     7201 tgcacttgca gaaaatgcat atgctcctgg attcaagttt ccgccagtta atttaggata
     7261 aaatctacct gcagctggag ctaaaatcga attgaacata cgatcttgga taatattatt
     7321 cgaatcataa aaatcaacag aaaaaacggc ctttttagac atagaaggtt gtaatttacc
     7381 cttggtcaaa attttgtttg gactcgtatc atatttatct ccgttttctg tcatcttcgc
     7441 gacattagct gtaacatcaa attgaagcgg gatatcgtcc acgttgaatt cgcctcgcgt
     7501 aataatagca ataatatctg tcaatagagt atttgtatcc cacgacccca tcccaaaacg
     7561 caaggtatgg cttcctgctg ataataaact tccgctagta accttgatgg gggcaccggc
     7621 aatatacaag taggcgttgt tccagtcaat tgcttttaat acatcactgc ttgatacatg
     7681 ttccggcaaa ttgatgatcg ccacaaatgc aaatccttgt gcagtgatat tcaccaaatc
     7741 aagtacattt gttagtttca catggctttc ccaatgaaaa cggaccgtgc cacctttcca
     7801 aggatcattt gaaccatcgt tcaaataagt agcagtcaat ttaggcaatt ggttagcccc
     7861 agttagttgg acaagcttcc cgccattgac tgcactacgt ccaccatttg gaggtacgat
     7921 attttcatac gtgtatggat cataagaaat ggcaggaggc gttgctccaa cagcttctgc
     7981 cgaaggtgaa aagataaata tacttaaaac aaaaagcgaa gtaaaaaaga aggagcgaaa
     8041 aaatgaaaat ttattttgta gcaacatgtt catcctcctt ttcctttctt ctccataaca
     8101 acaaccacgc aagaagccat aattctatac caatgatcaa ataaagaaaa cggatctttt
     8161 gttcattcgt ctccgggaat acgccatgtt gattagattc tttataaatc ggtagctgtg
     8221 tctgctgttt tgatgcgcca ttgactgaac cacttggatg cggaattgga cttagatcat
     8281 aaaatgtaat cgtacctgaa cttttggtat ctgcttggac tggctcttgc cagctgaaga
     8341 acagcaaact tgcaataatc cctcccaata agatcgatgt tttcaacgaa atcacctcat
     8401 catgttgttt ctcctttttt ctttttcttg tggacccaca aaaacagcaa aatcaaccat
     8461 aacactagaa ctgcagcaac caatcctgca taaagccatg ggaaagattg ttcttgtact
     8521 gcaaaaacgg aattttcatt tactttttgt gctttttctt ttttgatcgt aaagttttgt
     8581 gtaaatcgcc attcattact gtcgcctgat tttgctatga tttctgcgcg gtaatcgccg
     8641 gcttttgctt tatcaccagc taaatcaaga ttcaagtgaa aattcgaatt tggtgcaact
     8701 aaaaatgcat tagacgtatt ttcaatataa agctgatttt cataaaagat cttgctcgta
     8761 aatatcaaat tgctgataat cctaggctga ggattttgga aacgaagctg aacagttggc
     8821 tttgcgttga cctcgtccaa atctcctgat aaaagtttca gatccggttc tatcgtctct
     8881 tgactttctt gtaaaacgac tgcgagatta taagcaatac gatttttgat ttgtgctcct
     8941 tctgtagcat tttctgtttc agcttcttct ttaggtgaaa catgaatacc cgctaataca
     9001 cgtcccttaa agctttttgc tgggattttc acttctatag gtacagttgt ttctgcatgt
     9061 gctgatacat tgatttcctt tttcgcaatg agcaaaacgt cacggacatc aaacggcaat
     9121 gtctcatcta acgttgggtt tttttgacta taatcgatcg ttccaccatc actcgttacc
     9181 gccggattga cagagacctc aaatgttcgt tcttcatctg aagaattgct gataattact
     9241 tctagtgtct gattttgttc cggtgccact aataaatcaa aataaccgat tgctttatct
     9301 atttggttct ctggaaatac cggtttgatt ccaaagtctc ctgctgaatc ctttgcttgg
     9361 ctgattgacg gaaaaatgaa aaatagactt aaaatagaaa aaaacgaaac aaacagtcgt
     9421 ttactttgat tcattgtcca ccacatcctt tcaacaacaa ctgcggtaaa aaatccttca
     9481 cctatagtat tttcaccgca gtttgttgaa tttatttcat actctatgct actgggccag
     9541 ccacgaggtt ccaagtcaaa tcagccgtga atgttccagc tgctgctttc ggcactttca
     9601 gtgtgatatt tgctggatca taaacagaaa gccatgttcc cagaccttga tcttttgctg
     9661 ctgtaaatat tggtgtcgct gcacttgctt tactcaaccc agttacagtt tgactagttg
     9721 gtccagtgga ttcgttatct gacgtacttt taacagtgga tgttccaaaa tctaattcag
     9781 cgccttgcaa aacttgtgaa ccgtttacaa aatctgaaac cgaaactgtc acgttccacc
     9841 cttgtgcttg gccatctgca cctacacctc gacgatctga tacttggaga taaggtgtat
     9901 cgttttttga agcgtctact tgatacgttt ttgttccgct ttcaatctcg tgtgtaccaa
     9961 atggcaattc gggaactaca tccagtgtta aaggacctgt ctggccagtt gctggatttt
    10021 ccggatctgc tggctctact ggttttgaag gatcagtagg gttaagaggc tccacggtat
    10081 ttgttggttc agtaaaggta atatctcctt ttgaagtcgc ttgaccctca gcaaaagctg
    10141 gtgttgccaa gccgcctaga agaagtgttg aaagtaataa tgtcccaatc atgttctttt
    10201 tcataataaa tcactccttt ttcttagtga cctaagtata gtagagacag tatgcaaaaa
    10261 atcctcatga attcatccgc ttacttgaaa ttatgaccct ttttgtataa aagagaaaaa
    10321 aacaataaaa tcgtttacat gaatattctt ccttttttat taagtttcgt ttgtcaaatt
    10381 aagttagaca aatcatcttt tgtgacgtaa ctcaaaaata cttccattgg tgtccgatag
    10441 tgtaatgatt ttctaggaat atgatttcgt ttcgatgcca ccgattggat aaaggtttca
    10501 gatacaccat taaaatccat agctttatac aaaccatctt tacgtaataa gccatttgaa
    10561 tgttcattca atcctcgttg agaaggtgtt cctggatccg caaaataaat tgaaatatcg
    10621 ttggtattac taatcttttt ccaatttgag aattcttttc cacaatcaaa tgtgatggat
    10681 ttaaataagt tttttgggac acaacgaaac cattgattca gcctattttc aatgtcttta
    10741 gcctgtctgc cttcgggttt caaggtaatg atgactttag atagccgttc aactaatgtg
    10801 atgaccgcgc ttttatgttt tccacccacg atagtgtcac cttccaggtg accaaattca
    10861 tgatcaaact gggtatagtc tgtctttctg tctcggattg accggcgaaa cccttgtttt
    10921 cctctttttt ctttatgacc gtttggtttt ctttttccct tcattggtaa atcgtgagtg
    10981 tcaaaggttt tatctttaaa cattcgatac agtgtgcgta ccgaacaagc aataggaaac
    11041 tctgctcgac caataaggac atcaggagcc cacccttgaa caaccttttt ttgaatgtat
    11101 tcctgttgct catcaggtaa aacaatcgga tgtcttccgc agttacgttt gtttttctta
    11161 tattgtttat agtagctcat tgcgcttcca ccagatttaa gaaaacgata gaccttataa
    11221 atagtttgtc gagaacgttg taacagattg gctacttttg aaacaggttg tgattgattg
    11281 aaataagctt ctatcattac gagttcagtt ggtgtaagat ggttataggt catttgtact
    11341 cactccttat aattctttcg tcggaactat tttgagtgta gcacaaatga ctttttttag
    11401 ttgtctagct taattttaca atcggcgttt taaaggcagt aacaggatta aacattgaaa
    11461 cataatcgtt actaaatgaa atcaagaaat atactgtatt actcaattca ttgaaaatat
    11521 ttatcataat ttttaataga aaacactatt aaagagaaaa aagagaggta aatcaaatga
    11581 aaaaatcttt aaaacttgca tcagcagtta tattaatgtc ccaactcctt atagcaacac
    11641 ctttccatgt gttggcaagc gaaaattcaa catacaaaat tgcaaaagaa agaaccgaga
    11701 cgactgaaat gagtaattcc gatgtaacag cagagagtag tgaacttttg acaacttcat
    11761 caagtgaaag tagtacgact tcaaatgccg agtcttcaac aacttcatca agtgaaagta
    11821 gtacgacttc aaatgccgag tcttcaacaa cttcatcaag tgaaagtagt acgacttcaa
    11881 atgccgagtc ttcaacaact tcgtcaagtg aaagtagtac gatttcaagt accgaatctt
    11941 caacaacttc aagtagtaca agtgaaataa cttcttcgaa agtgacaaaa ccaaaccata
    12001 tagaatcttc aaaaaaggaa gcgccaaaaa cggaaggaca aacaaaaact ttttcgacac
    12061 cacagcaaat agaatcaaca gaatctttcc aatcacaacc aatactatct ggtaatgaag
    12121 aaattcattt tgacaaaaat gaatctgtag aaaattttat tatgaaaatt ggtgagtctg
    12181 cgagaaaaat tggtcagaaa tatgatttat atgcttctgt tatgattgca caagcgatct
    12241 tagaatctgc cagtggtcaa agccagttag ctcaggcacc gaattacaat ttatttggga
    12301 taaaaggaac ttataatgga aactttgtca ttatggtaac aaacgaagat ttgggtaatg
    12361 gaacgcttta tacgacccaa tcaaaattta gagtgtatga aaactatgag gaaagctttg
    12421 aagattatgc caaattatta actaagggaa ttagtggaaa taaagatttt tatgcaggag
    12481 cattaaaagc taatagtaaa acatatcgag aagcaactaa attccttaca ggacggtatg
    12541 caacagatac acaatactat ttgaagctaa atgaattaat taaaacctac gatttaacca
    12601 actatgataa agaagttaaa ggtccacaaa tgagtaagac tggataccaa gtgcctattc
    12661 ataattattc aataaccagt ttgtttggaa atcgtggaaa tgaatttcac cgaggacttg
    12721 atatggcagc tacacagggt gagccgattc atgcagttaa agctggtaag gttgttaaag
    12781 cagagtttca ctactcttgg ggaaattacg tggtgataca acacgaagat ggatcaaccg
    12841 cgttatatgc tcaccagcaa caatacctag taaaaactgg agataatgtt ttgcaaggac
    12901 aaataattgg ctatgtagga agtactggga atagtactgg cccacacctt catttggaaa
    12961 tttgcaaaga tagtagtctt tctcagagca tgttagttga tccgaaaacc ttattattcg
    13021 gagactaaaa aaggtaatca atctgacaaa gtaacaagaa aatggttgga ttcttagacc
    13081 tttgatcagg atattgagac tggaattgat atctcgatta cgatgggttt gacaaataaa
    13141 acaagtccat tatcgaatat cgagtgtatt attgccgtct ttatgctcat attttgagca
    13201 ggtttcacta gatgaaaaca atttatctat cttgaggatt tcgtgtacgt atcgttcagt
    13261 cttgtattgt aaattagtca taaaatcaga ctaagaaaaa ttggaaatat ttttttctga
    13321 tttattaaaa gttaattgaa attatatggt acttctttct tgggagggta ggtgcagtga
    13381 aggaaagata tggtcacctt actgttgttg aggagattac tcataatgga tataaaaaat
    13441 atcgttgcct atgtgactgt gggaatgaaa cgattgtata tcgatcaaac ttactaacag
    13501 gtagaaccaa aagttgtgga tgtggaaatt ataaaaatag gcataaatat aaaaatttga
    13561 caaataaaac ttttggtaag ttagttgcaa tcaagccaac taaactgcga agtgaaggaa
    13621 ctgttgtttg ggaatgtcaa tgtctgtgtg ggagaaaaaa gttagtatcg gcaaagcgac
    13681 ttctaaaagg tgatgtaaaa agctgtggat gtttgaaatc aaaaaagcat cacattgaaa
    13741 ataaaaaatt cggattactt actgcaaagt atcctttgga caataactgt tcatggaaaa
    13801 caaaatgggt ttgtcattgt gagtgtggca gtatgtgtga gataagttat agtaacttat
    13861 tttttggaca tactcaaagt tgtggatgtt taaaaaggca agaaacaagg acatgtgttg
    13921 aagggacagt aatagagtgt ctaacgtcaa aattaccgaa aaataataca agtggtgtta
    13981 agggagttta tcgttctaaa gagaaataga ttgcgtatat aactttaaaa aaaaagaggt
    14041 actatttagg atcgtatgat tctattaaga gtgcagctca agctagaaaa gatgctgaaa
    14101 ataaattgtt tcaacctttg attgacaaat atcgaggtaa tcattaatta ttgtggataa
    14161 taattaacag tgatatataa acatctataa tatattattc atactattga tatttagtat
    14221 aatcctatgg aattgaatat catagcaatg tatttgaaaa gcaaattgga aaacaaatag
    14281 gtgcaacacc ataaatatga aaaaattggg aaaaagtaga atggaaattt gcttacgtct
    14341 cacgagatag ctcacactat taatctgaag aacaatgaag tcgactttta gaaattgcta
    14401 gaagaaagta aagcgaatta tcactagtta ctatagagta agttcaaata aagcatctcc
    14461 aacttacatg gttacatgag gatatcaaat cgaaattact caagcttcct atggctagag
    14521 tcgcgagaag ttgttgggca gtagacatag gttatattgt tcttagctac acgatcatta
    14581 gaaaggaaat cctattatgt atagttatca agaagatgca aaagtccaaa aaaatcgctg
    14641 gtggattttg attgctatat ctatgtttac cttcatgtcc acattggatg gcagtattgt
    14701 caatattgca ctacctacca tctcagatga tatgggcata ccaatgaatc aagcggaatg
    14761 ggtagtgtcc gtttatctga tgattgtctg ttcttgtttg ttgctgtttg gtaaaattgg
    14821 cgatagccgt gggaaaatca aagtatttcg gattggtacg ctgatctttg tcttgggatc
    14881 gctgttgtgt gggttcaatc attcgctagg tattttattg ttcgcacggg tggtacaagc
    14941 cattggtgct agtatgacta tggctacaaa tgccggaatc atcacggaag tctttcccat
    15001 acatgaacgt ggtcgggcat tgggagcaat aggtgcgttt gtttcattag gctcgattgc
    15061 tggaccaggt cttggcggct tgattttgtc ccaattttct tgggcgtaca ttttttggat
    15121 caatgtaccg gtgggaatcg taacgatgtt gattgctgaa aaattcttac caaaagatat
    15181 caccatgagc ggccatacaa tcgatggagg gggctttgct gccattgcgg ctacgattat
    15241 cagctttttt ggagcaatct ttttggggca agaaagtggc tttttccatc cagtccccct
    15301 tgtactgttc gtaatttcag tgattgcctt tgtcagcttt ttaaaagttg aaaagaaagc
    15361 agcctctcca ttgatttctt tcggtatttt taagaacaag atttttacca tgagcttgat
    15421 tacagccttg ttgatttttt cttccaactt ctttgtcaat gtagtgattc ctttttatct
    15481 gcaaaatgct cgcgggttga gtcctagcta tgctgggctg ttgatgatgg tctttccttt
    15541 gatgatggtc gtcggggcgc ctttaagcgg ctacctgacc gaccgggttg gcccggagat
    15601 tttggtatta gtaggcttgg gcttattgtc ggtaacccag gtcatgtata tgtttttgtc
    15661 agtggactct cctttgtggc tatacattgt cgctactgcc atcatgggtg ccggcaatgc
    15721 cttgttccag tcccccaata atacgatggt tatgagctcg gtcactatcg aaaacttggg
    15781 actggcaggc tcgatgaatt cctttgcccg aaatttgggg atggtcagcg ggatttcgct
    15841 ttctacgacg gtcttatatc aagcgatgtc tgctaagatg ggccagcaag taactaacta
    15901 tatcgcaaat cggccggatg tctttttata tggaatgaag attacctttt taggcagctt
    15961 tgttttgtgt tttgttgcct ttttcctgac tatctggcgt atcaaaaaac aaggaggtat
    16021 tcagaaaaat gtctgaaaaa accactgttg aagaattttt ggaggcctgc gtatgggtga
    16081 gtgggatttc catggatgat ggaagatgaa gtgaacttct aaaagccgaa aaactcaagt
    16141 attatcttca aaatactagt gctcaatgag atgcttgatt tgatcacaag aagactgcga
    16201 aagccgctgg caaccgcaag ctaggtatcc caccgatgga ctacgtttta gaggctactg
    16261 gttatgaata cggagccaac gctctcatct gcataagaat acatcaccca gattatgtaa
    16321 agattttcga tacttctgtc aaaaaatcat taagatagat aaatgatttc cgtctagtca
    16381 aattggttca gaatgtagac acacagatgg taagaagtca ctcacaattc gagaatggac
    16441 ttgtcctatt tgccgtatac atcataatcg aaacgtcaac gatagtatca atattttgac
    16501 caagggctat gaatacaagc attgccttag aaaacatcaa gaaccttagg aacaacgggg
    16561 atagcttggt taataagaga taccgctgtt taggcttgca agtatactct tttatcaaga
    16621 attccgtggc tttagtcatg agaggcttaa tactatgaaa attttcttta gcatttttga
    16681 tttaggttcc ttttctaatt ataataagat gaccactaca gtaacctact gaaatatata
    16741 aacagccact gtagtaccat tttcaaagta gtctatgaga ggagaatctt cattcttatg
    16801 aacgctggaa ttgcggatat gaatttaata aaaaaaaccc ttaatgattt tacttccaat
    16861 tcaatatcaa aaggtactgg tatcaattta agcacaataa aaaagctgaa aagtggtgaa
    16921 agatcagtgg aaaagctgaa tcttttagat gctataaaaa taacggaatt tgctatgaaa
    16981 aatggaaaag ctgagattga aatatggaga taattatcgt ctagtgaatt acttgccact
    17041 tagctgaatt taacgatcct tggtgcttga agggggagat tcttgggaat agagcatact
    17101 tgcaagccta tgtctttact aacaacgata tctcttatta accaagctat ccccgtagtt
    17161 ctaaggcttt tacttttatc taagcaataa tcgtattcaa aggaaatttt tgacccttag
    17221 aaagtctcat tttatcagac gtgagcttag gcattgctga attttgttaa ttcggtttta
    17281 ggttgtgctt tgagcataat atgaaagtat tctttatcac gatttcattc atctaaggta
    17341 atgtgatgtg attctgaaat tctttcaaag gcagtttttg cataagctga cattacatca
    17401 tcaatgacat gtttacaatg tttcactact aaaataaggg ggtaaccaag aagaaaaact
    17461 gaatgattat ttgtatctaa ttccatggta tatcaaccat ttcctatata gactgattat
    17521 gacatggatt aaaataaaaa ggcactatgt cttaattgtc ggttttcgag ttaccgaatg
    17581 gcattcatca cccacctata gaggatgggc gacttctgcc tgaaataagt tacactactt
    17641 ataacttcta ataaaattag attgagtcat tattatttaa catcaacgat tttccagttt
    17701 ccattttttt gtaatactaa tttgtactga aagtagctag tggtttttga tacattatca
    17761 aggtatttta tctttgtact cactagaaaa ttttttccgt tttgtagata aactggattc
    17821 tctaactccg aaaactttaa gtcaatattg attggtcgca tcgcaccatt ttcaacataa
    17881 tactccaatt ctttttcaga agctgatgga taaagtttaa aaaatgtttc taaaaaatcg
    17941 gtgatttctt ccgttgtttt ggcatcaatt tctgaatcgt tttctacttg ttttgcttca
    18001 tattttgcct tgtctggctt tccaatcatt gttggcaaac tagttactaa atgattcctg
    18061 ttttcatctt caaagattgt cactcgatat gcagaagaaa ttgattgcgt cttttttccc
    18121 tctgtaatat gttgctccac taaaaatgaa acgacaaatt cttccgagtg tttttcaaca
    18181 tctagaattt ttacagattg aacttcggaa ctcgtgggaa tatctgctcg aaccatatct
    18241 tggattaaag cgagaccgtt ttctgtaagg tactgttcga gattactcat tctcttttca
    18301 attgcttctt tgttattttt ccatgaaaaa tattctttcg caaaatcctc tgtaaaactt
    18361 tcaacgccag aagtattcac taccttctct tttataacca ctttttcatg agttgtatgt
    18421 tgatcaatgg cggtgaaatt tttataaacg ccaaaagcaa gactacaaga caataaaagc
    18481 caaagggcaa gcaccatttt tcgatgctta cccacggata caactcgttc ttttttcttt
    18541 tttgggctct tttccttttg attccgttca atttttatct tcattctttt tcctcttttc
    18601 tcttatttgt atcggccagc tccgataaga tgctgccgcc aatagttttc cgttagattc
    18661 gtccacccca ctggatctcc tgcatgaaac atgcgattct cccctgcgta aatgcccaca
    18721 tgggtcacat aagtaccggc atcataggtt gaatgaaaga aaactaaatc acctggtttg
    18781 gcatctttta gatcaatatg agtcatggca tcatattgat cctgagccac ccgaggaagt
    18841 ttcaggtcaa ctttggcata acaccattgg gtcaagccac tacaatcaaa ccccgtttct
    18901 ggacttgagc caccaaacac ataggccgtt ccttcatact taaatgcctc atcaaaaatt
    18961 tcttggacta ttttgtcatc aaactgagtt gtagttaggt attgcttcac taataaacag
    19021 taaaacatat tgccataatt ataccgccac ccaccatttt ccttaatcgc gattgggttg
    19081 gggtaatcga ctttttgacc acctgatttt tcctttgaaa aagattcagc taaagcaaaa
    19141 ctatgcttct tcccgttttt cccgacatga tctagatacc cgccaccata gttataggcc
    19201 tgaataatac tatcttgatc acaccctagt tctttggaac gcttaaccaa agacgcaaaa
    19261 tagagacagc cttgtttgat ggatgcctcg gtacttaatg aattaggtgg taatccggct
    19321 gactcagaac tttgcataac gtcttccatt gtgccaccag attccacttg aataatcgcc
    19381 aaaagaattg gaatatactc tgttatatcc gattctttgg catacttttc caccattgca
    19441 cggtgtttca atacttcttc tgagacacta gttccgccag gagaagtgcc agaactacca
    19501 tttccctcac tgtcttcacc aaacaacaaa cccacacaca tcaaaatacc cataaaagag
    19561 atggtcagaa aagttaatga aataacaccc actttcttca agttcatttt ttctcacctg
    19621 accttgattt tggacgatgg ttcattcttt tttgtacttt ttgattggtc tgctttggca
    19681 gagtggacct ctgtttcact tgttggaaag aatttttctt acttgctgat tggcttttcc
    19741 cttttgaaat gatttcttga ctagttagtt ttcgtttgat ttcgggattc agatttgctc
    19801 tcacaatccg ctttggtgct tcttttttca gcatcatatc tttctgaggt tgagctgctt
    19861 tatctattgg aatattcgcc cttctttttc cctgacgatt tgttgcttca ctcagtatct
    19921 tgcgtttttc gtccattttc gcatttcttc gttgtcgttg tttttctcgc aaatccactc
    19981 gatccgcttt ttcttggaac agtccgcgct tgaattcttc tggtgctttt ttcgactttt
    20041 cgattccttt atggagattg tatttcaaat tcgttggcgt attccttacc tgatccttta
    20101 cttgtttagt cttatctttc accaaagctt gggtatccaa aactttgccg atttttctcc
    20161 cagccaaatt cattcgggat tgtttcttat tatttgaaac tggattctcc ttagttaagt
    20221 cagatagtat ttcgtgatcg ttcggtaatc gtgaattctt tcgtagagaa gaaccggatg
    20281 gcttaaattt ggattttgat tgttcctttg ccaacttatt tccgactgct gcaccaccac
    20341 caaaagccaa agctcgtccc acatttcgat tgagttgccg caattttcga ttcattaaca
    20401 tttgcggttt tcgcatgacg cgtcttccca ttgactggct atcattactc tggaggttga
    20461 acatactcat aatgtcgccc aatttgaaat agataccggc aaaggtcaca atttgcagaa
    20521 aagcaaccat gaaaaatgga taggttgccg aaatattgaa aaacatcgtg gagatgctaa
    20581 atgcagtcgt aatcaccaaa gtaacacccg ctcgcatcat aatcgtattg aataaacgaa
    20641 taatcgcctt ctttcctaaa ctttcataag tcggcaacat ggacaataag aaactaattg
    20701 gcagaaacat cgcaaagata atgaagagaa tttgcgagaa caacataatc cctgttagaa
    20761 gaaaaacaaa gaaggaaatg ccaatattga acaataccaa aaagaccacg gtccctaaac
    20821 ggttcatggt cttggtaata ctgagattcg cattgtcatt gtcttcaatc tctgccttga
    20881 ccgcttcttc ccgatctttg cctttgtttt catctggact cacagataga atcttgttga
    20941 cccggtcttc accaatttct tctatgttgg aatcatcaaa ttgcagtagt aaccaaggtt
    21001 gttggacttg aatcgaaaac aaactatccc gaatcaaatc cacactatct tttccttggc
    21061 tttctgaatt gggtacaacg atctcggttc caagggtaag ggccgcttca ctaacatctg
    21121 agctaaagtc attgattttt gtgatatagg taggcgcata agcaatgaac gaacccgata
    21181 gtagaaaaat catcacaaaa ttaatcacgg ctcgaactgc tttcgtggtt tctcgtttta
    21241 ataatccggt atacgccaca tacaccccca gagctaaaat catcaataat agaaacccaa
    21301 aataaaatcc ggaggtctgg agtccatttt cagtaatacc agccaatgtt tggatattct
    21361 ttccaatact ttcagccgta tccgaaatga aatctaattt gtaagcctct tgaaccacat
    21421 aacccgtggc gttcgacaaa tacaaactca ccgtccaaat gaaattagta atggcataaa
    21481 gaccatacat cacgcttttc cctaagccat caccccaatt ccaaggcaac caatcccaag
    21541 agctatccac aaagaaatct agttgatagt tgttcagtga atatttggaa tatagatttc
    21601 ctgcttcaac tgtatcatca actagccctg tcgcctcggc taccgtacca actaaactca
    21661 atagaactat cagagtgagt atgctaataa aaacgattcc cacaatccga aaaagtttct
    21721 ttttcatccc ctcactccat tctctcttga acaggtggac gggtatcaaa ggcatggaag
    21781 agttcttcaa agactggatg tacctgaacc acgcctaccc gaccatacaa atcctgcatc
    21841 aagcattggc cattttccaa atctcgtagg cgtttttgat tgctttcatc ttctcgatcc
    21901 aagccaaaga attccaaggt tttcttgatt tcatgaatat cggtactgcg aaacgcaaac
    21961 ttcaacccga tattattctt caagcgttca tccagtaaat catcactatt ttgcgtgaca
    22021 aagtaaaccc cggcattcat agcccgccca gcacgaacga gcttattcga taatgttttc
    22081 ccttgcgcta cttgaagaaa actccacgcc tcatctaagt ctacaatttt gaagatttct
    22141 cgattactat ggataaagtc caaggcaaaa gtggaaatga cgattagcat ggaaacacta
    22201 agcaattcca tggtggtata ttcttcaaaa gtggtttctg catctggtaa aaccaaatcc
    22261 gctacttgaa tgatatttaa ctgcctatct aaactaatcg actgggtaat cgaaccatct
    22321 gagaaaagca aatgggcaaa atcataatcg gtaaaggact cgatgtgatc cgcgatattt
    22381 tccgcaactg gtgagccttc ttttcgaagc tcattaatta cttgtaataa tccccgttga
    22441 ttgctttggg taaccgtccg aatggcctta cgcaacactg gaaacttctc cccatctcga
    22501 ctagaaattc ctgttaaaaa ggtcagaata tcaattgcta gactttcaga atccttcttt
    22561 ttctccataa tcacataagg atcaagtaat ccttgatttt ttggcttact agttaagttg
    22621 agaatattga tttcatccgc aatttctggc aaggtttctt tccagccacc ccgttcccct
    22681 tttggatcga caatcacagc ttgtccacca aaaaggacgg cgtaataaac taagagattg
    22741 ttactaaagg actttccgcc accgagtgaa ccgaggaatg ccgctgctag cgcattggta
    22801 actgaatcct tgaccccttg tgaggccaat gccggtttca ggtaaacatt gcgccccgta
    22861 tctacgttat aaccaaagta aatgccttcc aactccccta acatctgagt ggcgccaaaa
    22921 ccaagtccgg cgagaaaatc agacgtaaca tactggatat aatcattcat gtatcgctta
    22981 ctagaaggta agaattcttc atgtagcccc atcatatcgc caaaaggccg gactagtttt
    23041 acattggtat catcataaaa atctagcact tcatcacacc ggcgctttag ttcatctact
    23101 gattcggcac ttactcgaac cacatagctc agtttgtaca tggattcttt ggactgatcc
    23161 aaggtagttt ccaattcatc tacggaatcc aatgcatcta agacattgga atttgtttca
    23221 ttgtcagatt gataggcatg gttgtccaag tcttttaatt cctttttctt attgcgaacg
    23281 gtagcaagtg cctttttgtt cgtcacaatc tccacattca tactagtgtc aattgggaat
    23341 gtaaattgtt gttgctggta atagaaaagt tctgaggagg gaaactccat ttcccccaca
    23401 attgtattaa ttgttaagta cgctacatat gattcatgat tctcatgttc catgcataag
    23461 taccggggct tttcttcaat caaacaacga ctgggacgta ataagtcgta acgtttcacc
    23521 aaagtttccg acttcagctt tttctttggt agctgaaatt catattcttc aaatggtgta
    23581 cccttttcgc catagatatg ttcaatcaaa tacatcagat cactcggtgt gactcgtctg
    23641 actttaaatc ttcgagccaa tttactttcc attagttttt caagcttggt atatcggcga
    23701 atttcttcat tggataagga aacaaaatcg cccatcaaat gatgattcac tccatagaca
    23761 aattcttgaa agccggagaa gaaagatttc ttgagattct ttagattgac ctcttcatct
    23821 gtcgcaatca gtttgaagcc aataaaaaag cgataatcaa tctgactatc gccaatcatg
    23881 gatactaatg cctctgtctg taaatcaatt cgttgtttcg ctacttctgt taatcgcccg
    23941 gtaatttcct tttttgatcg ttcttgcgtg gcacgaatac tactttctgt ggcaatttgt
    24001 aaggcatgaa tcttgccttc acgattttgc gcaatcaatt gacgaaagtt atcgtggact
    24061 tgatatttct gttcaggtga caaaaaggag taattgtaag gtattaactc atagtaggca
    24121 aaacattctc cctcttgatt aaagacaaga ttattttcaa tatacttaat cgggtacata
    24181 gttcacactc cttaccagag taatgggttc gtcccatcgt ttcttctgtt ctttgacttt
    24241 ctttcctgcg aaagtgacct taaatcgtaa gtgatagcta ataaaagatc gtaaaaatcc
    24301 caatggtttc ttaccgtcaa acgttttttg cgacatgaac caagtaactg ccaccggtat
    24361 acctaagtat tttaaaaagg ccccctctat catagatagt ggaggcacat gggcaaatac
    24421 gatgacaaac agcaaggata aaacaaacca agccatctgg ttaaaagtca caggaaaagg
    24481 cagttgaaag tcattaattg cataaatgac cttttcaact gcccaaatac tggtgtagct
    24541 ttttattttc ttcatcaaat cattccttta aaaagaagga tagtttagaa atcaactacc
    24601 cttcaataat tttgttaata tggatactca aaaacacctc gactcgttac caaataattg
    24661 cctccaatct ccaagtctct accacacgca tcaaaatcaa tatacgtctt caagtgttct
    24721 ggtacttctc caaaatcacc agcttctaca atcaagaaat atgctacatc actcatatca
    24781 tcgcaatctg gataatagat aatatcatcc acatgctcta ccatctcctc aaaagaattg
    24841 aagaaagcat gttgaatctc agaagcaact tcaccaactg gcgttccttc aagttcctcc
    24901 gctaagccac acaaccgatt aatttcctca attggcgtat actcatcaat ctcaaaggga
    24961 agttcataat catgaattgc atattcctcg tattcgtcat ttaacccaat tcgttctttc
    25021 acttcatcaa aatcgacagg tggggtaaac cacgccccca ccaattcacc ttcgttatat
    25081 ttcccgaggt tcgcaatata aacacgcatc tgttccatcg tgcttccccc ttacgcaccg
    25141 acaattcggt taaacaaact gagtaagaca tccttcaccc cagaagcatt aaagaccaat
    25201 ccgaccgcaa tcaaagcaat caccaaaaag ccaatcagtt tggaaaactc ccgcttaaat
    25261 cctaaaaaca aaccaatgac cacaattgcc agtaagacta aggattgggc attggttaaa
    25321 aaccattgat atatggatag gtaagttcct gatattataa atatctcaaa cttttccccc
    25381 cattcacacg gagcgtgcga ctttcaccgc actccgcgtt ccatcgtgtt tatattgaaa
    25441 agtatttatg tagtagacct atatcaagtt taattcttga ttgccagcta atactctata
    25501 tttatttagt cttatgagct ggactttatt tagcttcaat agagctatat atttttgtat
    25561 tgtttcttct gttttagcgt gtattagacg gtgtaccgcc tcttttacca aaataagatt
    25621 tttataatcg tcatttccac cagctccagt tggtacaata tgatgacaat gtatttcatt
    25681 agcttgttga aattcttcca gagttatagc acattttcca tgttgagcag aaaatagcga
    25741 aattcgattg tctgcgtatt ctattgtgcg attttccaaa gataatttca ttaactggta
    25801 catcagtaat ttatcaacac ttagattggt gtggataaat tttcttccct ctagagtgta
    25861 gcaattcgct aaatacacct ttgccattgg ttttctgtgt tggatatatc caacagggaa
    25921 aataggttcg ccctcgccag caacataccg aagcatttta gatttaccat atcttgttgc
    25981 ctcctttgtc gtaagttgtc tgcctgtttt tctcaatctg ccccttttgt ttgtacctaa
    26041 tctgttagtg aagatagcca ttacagctct attcagtttc atacaatcca gatttacatt
    26101 cgttgcaatc tgataataat tttgcatacc taataccatt gaattataaa gtcttgtttc
    26161 ttctaattcc gttttccctt ttgagggaga agcaattttc tttgcctgtg ataccagctt
    26221 gtctttttgg tttttgaatt gtttatcact tatatgtgat ttaacaacta acttctttct
    26281 tctcggtatc attttaatct tgaaaccaag aaagtcggag tatctttttc taacatttac
    26341 aattcttgtt ttttcttgtg atacttccaa ttttaatcgt tgttcaatcc attgagtgat
    26401 tgctattttg gctttttcag cactttcctt gtagcggcag aaaactctaa aatcatctgc
    26461 ataacgaacc atatacattt cctttaactt tgttttcttc atttctttgt aagcgttgct
    26521 tttacatggg ctaccgcttt tattgatatg aaccacatag ttttttgtaa caggattttc
    26581 ctgccattgg ctttctaccc aatggtctaa ctcattaaga acaatgtttg ctagtagtgg
    26641 ggatattatt cccccttgcg gagttccttt ctctggatat gttattgtac cgttagtcat
    26701 tttgattgga gcttttaata tcctttttaa aatgaatatc aagtgcttgt cccgtattcc
    26761 catagcccat atctgtttta tcagcttgga atggttcacg ttgtcaaaga aacccttaat
    26821 atcaaattca atcacataat gtagctggct gatttgtagg cggttatagg ttgcttttat
    26881 tgcattttca actgacctat taggtctaaa accatagctg ttttcactaa atttagcttc
    26941 gcagattggt tctaatactt gttttataca ttgttgtata agcctgtccc acatacaagg
    27001 aatacctaga ggtctggttt taccatttgg ttttggtatt tcttttcgcc tcactggctt
    27061 aggtcgatac ccatgtggac tgcctgcaac aatgtatctt acttttttga caactgtttc
    27121 tgctggcaat ttgccaatat ctttaattat tgtaccgtct gttcctgctg ttttactgcc
    27181 tgcatttgct ttgatatttc gatatgccag taatatattt tctctcgata ggataatatc
    27241 cataagatga gtgaaaatat ctccattctt acttttggca tataaatcat caaaggtgtt
    27301 ttgcatttcg taatattcag cgtgtcttaa atcttctaca cacaatgttt catttttatt
    27361 tttaggcata aggcttcact cctttacttg aaattgagtg agctttttta tcttactcgt
    27421 aatccttatt gaaacaacat aatacttttc taatatatac acgacttgag ggcattcctc
    27481 cattctcatt acaagaattt cttaggttcg ccctctgctt ttcagcatta gttcaactgc
    27541 ttattgttct tcgtcagttt attctcttga tgagaaccca atacctttcc tcgttccgat
    27601 aatcttatct atacatatat atccttaggt cactagctat aagcctgctg gcttgatacc
    27661 gcctgtaacg ttatatggca tttcataacc tatattctta ctttgccaca ccagctacga
    27721 tttctcgtac ctttgcattt ctacaaagtt caaatttaga cccgtacatt cgctgtttcg
    27781 tcagttcctt atagaacatt ctaaccatag atattttata gacccccgac ctgttggata
    27841 cgctacccac ctctggatag ttttcgatag aagatatatg ttcatcaact attacggtat
    27901 ctttcagccg tctttaccga gcttcaaaca ttctgagttt cctcatacaa tgcttgtcgg
    27961 aggctcaggg caggcgtttc agagcgttac ttcatcattc cacctgtcag attatcagtt
    28021 ctcctaataa ccaaactttt tcgttctttt attagtgcct tagctttt
//
LOCUS       DACDIG010000030        28062 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.030, whole genome shotgun sequence.
ACCESSION   DACDIG010000030 DACDIG010000000
VERSION     DACDIG010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.030"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(409..1353)
                     /locus_tag="IV247_001419"
     CDS             complement(409..1353)
                     /locus_tag="IV247_001419"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP8777496.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1649..2593)
                     /locus_tag="IV247_001420"
     CDS             complement(1649..2593)
                     /locus_tag="IV247_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8777497.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2814..4028
                     /locus_tag="IV247_001421"
     CDS             2814..4028
                     /locus_tag="IV247_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8777498.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            4050..6065
                     /locus_tag="IV247_001422"
     CDS             4050..6065
                     /locus_tag="IV247_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAP8777499.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6148..7521)
                     /locus_tag="IV247_001423"
     CDS             complement(6148..7521)
                     /locus_tag="IV247_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP8777500.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7547..7831)
                     /locus_tag="IV247_001424"
     CDS             complement(7547..7831)
                     /locus_tag="IV247_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8777501.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7833..9848)
                     /locus_tag="IV247_001425"
     CDS             complement(7833..9848)
                     /locus_tag="IV247_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP8777502.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(10027..10332)
                     /locus_tag="IV247_001426"
     CDS             complement(10027..10332)
                     /locus_tag="IV247_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAP8777503.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10421..11452)
                     /locus_tag="IV247_001427"
     CDS             complement(10421..11452)
                     /locus_tag="IV247_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAP8777504.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11659..12534
                     /locus_tag="IV247_001428"
     CDS             11659..12534
                     /locus_tag="IV247_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8777505.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12686..13528)
                     /locus_tag="IV247_001429"
     CDS             complement(12686..13528)
                     /locus_tag="IV247_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8777506.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13620..15497)
                     /locus_tag="IV247_001430"
     CDS             complement(13620..15497)
                     /locus_tag="IV247_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP8777507.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15494..16915)
                     /locus_tag="IV247_001431"
     CDS             complement(15494..16915)
                     /locus_tag="IV247_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP8777508.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16918..20307)
                     /locus_tag="IV247_001432"
     CDS             complement(16918..20307)
                     /locus_tag="IV247_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP8777509.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20564..21955
                     /locus_tag="IV247_001433"
     CDS             20564..21955
                     /locus_tag="IV247_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8777510.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYKLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(22028..22675)
                     /locus_tag="IV247_001434"
     CDS             complement(22028..22675)
                     /locus_tag="IV247_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8777511.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22683..23369)
                     /locus_tag="IV247_001435"
     CDS             complement(22683..23369)
                     /locus_tag="IV247_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8777512.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23375..24448)
                     /locus_tag="IV247_001436"
     CDS             complement(23375..24448)
                     /locus_tag="IV247_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP8777513.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24670..25344
                     /locus_tag="IV247_001437"
     CDS             24670..25344
                     /locus_tag="IV247_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8777514.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25349..26689
                     /locus_tag="IV247_001438"
     CDS             25349..26689
                     /locus_tag="IV247_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8777515.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26838..27824)
                     /locus_tag="IV247_001439"
     CDS             complement(26838..27824)
                     /locus_tag="IV247_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAP8777516.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8195 a         5281 c         5075 g         9511 t
ORIGIN      
        1 ctctcgaacg gttttttatc aaaaatcgtc atttttccat caaaatctgc ttatgcaaaa
       61 ttataaaaaa attttgcagt ttttcttact ttttttgtcg tttaattgaa tgataaaaac
      121 cagtttcttt attcagttaa tcaaatatga acgaattaga tatgaatcat tgtatttgcc
      181 gttgcggaag gattttttaa ttaaccacag ataaacattg ataacgattc gcttattgta
      241 atcaacagat taaatttcat atgttcatat gtacggatac attttcggac cgattaattt
      301 taaccattct gtttatttgc ttacctatta gatatcaata aaaaactgct taacagctat
      361 ttcagctgtt aagcagtttt aggattttta gttcatattc attgtttctt ataatacagt
      421 gttcaatact tctcttaaag cacttgttga tttgctgaac attgcttttt cttcttcgtt
      481 gattgataat tcaacaactt cacgaacacc attttcgtca acgattgatg gcacacctgt
      541 aaagatatct ttttcgtcgt attcaccagt taaataagca gatacaggca atacagcttg
      601 ttcgttattc aagatagctt ttacgatacg tgcagtactc ataccaatac catagtaagt
      661 tgctttttta cgatcaatga tctcataagc agcattacgg actttatcag caatcacatc
      721 taattcatct tgggcaatac gatggtcttt ttctacaatt tcaaacactg gtttaccacc
      781 gacagttgta tgtgaccatg cagcaacttc tgaatcacca tgttcaccta agatatagcc
      841 gtggacgctg cgtgggtcaa cttttaattt caatgcgatt tctttacgga aacgagttgt
      901 atccaatgtt gttccagtac cgataacacg tgatgttggc agaccagatt cgttccaagc
      961 gatatatgtc aaaatatcta ctgggtttga agcaacaacg ataataccgt cgaatcctga
     1021 tcccatgatt tcttttacga tttggcgcat gattgacgca tttgttttaa ctaaatctaa
     1081 acgagattga ccaggttttt ggttgatacc agctgtgata acaacgatat cagcatcttt
     1141 acattcttca tagccacctg accatacagc cacattttca tcgccccaag ccataccatc
     1201 taaaaggtcc aatgcttccc cttctgcttt ttcttgattc acatcgatca atactaattc
     1261 attagagatt ccttggttga tcattgcata agcgatactt gttccaacaa aacctgtacc
     1321 gacgattact actttacgac ttgttttttt catgaataaa cagctccttt tcataactat
     1381 ttgttacttt catgaaaagt atagtcttgt ttattcattt tatcaacatg ttaattataa
     1441 tagttgttat tataattttt gatggaaacg aattcctgaa tacgcataac agataaatga
     1501 caaatgttta cacctatatc ccctgataat acaaccaaaa gtttagaact gaaaataaat
     1561 ccaaaaaaat tcgctgcaac gatataataa gacagcaatc gcacctaaaa caattttcct
     1621 tcatatgctt tagtgaaaga ttttcttctt aatgtttatt tctccattca ttgggagtac
     1681 atgttgaata cttcttgaat aatttgaata aagaagtcgt ttgtttatat ccgatacgat
     1741 tagcgatttc ttccaacgaa aaactcgttt cttgaatcag ttccaacgca aagtcgaatt
     1801 ttttctttag aaccagttct tgaaaggaag catcaaaata cattttcaat ttattactaa
     1861 gatagaccgg attgtagcca aaatgagctg ctgtttcttt taacgtacaa gaagtaaaat
     1921 gcttatcgat ataatctaat actctaagca tctcattttc tgcgcatcca tctgctgatg
     1981 aaaagctttg ttgttctaat aaatgagtca attcgatcag taatagtgat gtatagagat
     2041 ttaacgaacg gatcttatga ctgtatgtat caaaataaat caacagcata ttttcaatca
     2101 aatgcgtcaa acgacttgtt tttccgccgc gaaaatgaat gaattgtgta tgttcccctt
     2161 tttcttttga tgcatttatc aaaaaagaag aaagtagatt atttgtgctt gccaaatgat
     2221 tcaatatctc tgtagataag gtttcttctt ttaataagat gttgattaaa atatcctctt
     2281 gccctgtgta ttcgattctt tgagtagctt ctttatcaat gatcaccacg tcactttctt
     2341 ccatctctat cctttctcca ttaataattt gtaaacatgt accgctatac atataattga
     2401 tttcaacaaa atcatgtgta tgctcgggaa cttccgcata tcgacggtgt ttactgatat
     2461 gaatatttcc ctgttgaaat agatcccctc ggtggatttt tggcggtgtc tgaagatggt
     2521 aagctaagtt taaatcattg atattcattc cttgttcacg ctgcagagtt tcgattgatt
     2581 cttcgttacg caaaaaaaat gtctaattca gctagttcca attgactccc tcctttttgt
     2641 aagcggtttc tttcgcttat tagttcacgc tgtactgcta ttatatgcaa agctattttt
     2701 ggtttcataa agactttcta tggttgggac atgaacttcc tgtgattccg ctattgagag
     2761 aataaccact tcatactata tttttgataa gcgcttacaa aaatgaaagg aacatgctta
     2821 tgaataaaaa gatgatgaaa ttttctctgc tgtcagtctc tcttattttg acttctgctg
     2881 gtgcagtcac gtctgcaatt cccagtatgt cagctactta tgccacgcgt tctttatctg
     2941 ctgttgaatt attaacgact gctccctctt ttatggtcac tatttttgtt ttgattagta
     3001 gttttattgc taggaaaatc ggacaaaaac aaactgtgat catcggatta gtgattgcat
     3061 cgatttgtgg tatcctcccc ctcttctcac aaagttacag tattgtctta gcttcaagag
     3121 ctggactagg tattggcttt ggattgatca attctttggc agtcagcatg attgctgcct
     3181 tttatcacgg agatgaacga gcctctatga ttggttttca aagtgcattt caaggatttg
     3241 gtgcagcttt aatgacattt gttgcaggac agctagtaca gttcggttgg caatatacct
     3301 ttttagtcta cgccatcagc ttacctattc ttgtcttgtt tgttttgttt gttcctacac
     3361 ctgaaacaac tgaacttcaa tcgcatggca cagccccaac aagacaagta atcaaaacga
     3421 atcctaaaat gattagttat ggtatttttc tcttgctggt catcgtggta tataatgctg
     3481 tatcgattaa attagccaca gtcctgacga cttattctct aggaaacgaa actaatgcag
     3541 cgaatatctt aacaatcatg caattggcaa gtatggctgc cggagtttgt tttggctggc
     3601 ttcagacaaa agcacatcgc tatatgctta cactttcttt actactgatg ggaatcggct
     3661 ttgtattgat tgccactgtg ataaacctgt atgtgattgg gtttggtgcg atcctaacag
     3721 gaatttcttt ttcgttattc attccttact tattcaacca agtaagtatc caagcgccca
     3781 agtacgcgga gagtttcaat acttcaatct tattggttgg cgctaattta ggtactttca
     3841 ttgcaccata tggtttgctc tttctctctt ttgcttctct tgggagtaaa acacttgctg
     3901 ttttcgtcaa tggcagtatc ttgctgttga ttagcgcgct tattagcctt ctagtagtac
     3961 acaggacgaa tagaaagaca gtgataagag tagatgaaga aataaaagaa caattgacaa
     4021 caaaataaat ttgatggagg tcgtgtaaaa tgacaaatga aaaacataaa gatagatggc
     4081 tttggtatcc tggagacttt gagatccgcc atggactgct acaaaatttc caacgggaag
     4141 aacgaggttt tgactggcca gcgtactggt acatggatga ttgccatcgg aacgtcaagt
     4201 ttaagagata ttactttctt gatcaacctt ccatgtttaa agtgacgatc caaggagttg
     4261 ggtatgtaga aattaatgga caaaaacatc catgtggaaa atggctgact tgtccagctg
     4321 gtaaagcaaa aattcgtatc tttgtaggac atacatcagg gctaccagcg atgtttatcg
     4381 agggagacga agtaaaatca gatattggct ggacagcaag taattttatt gaagaatatc
     4441 cagctggctg gagtccgctt tatgtcgata tcgcaaaaga tccaaatcaa atctattatc
     4501 aaaaagaatg cgtccatcca gtcactgaaa cggagattgg cggaggaatc ttattcgatt
     4561 ttgggcgagc agtgaatggc tgtgtagaac taatatctct ggatgaccat atcgaatcta
     4621 tcactctatg ttatggagag tccgatgtag aagctatgga tatcgattat tgctactata
     4681 agcaaatagg aatgacaaga aaaatgaaag ctcgtaaacg cgcttttcgc tatttgttta
     4741 taccagaggt ttctactgga atggttcaac ttcaagtttt tcatgaattt ttatctcttt
     4801 cgaaacaagc tgctttttca agcgattctt ctttactgaa tcagatctgg tcagtatcag
     4861 aagaagcctt caagttatgt agtgatttgt tttttatcga cggtatcaaa cgagatcgct
     4921 ggatttggtc tggagatgcg tatcaatcat tcatgatcaa tcaatatctt ttttttgacg
     4981 aggaaatcaa taaacgaacg atattagcat taagaggaca aaatgaaatc aagcagcata
     5041 tgaatactat cgttgactat tcgatcttgt ggttgattgg tatcgaaaac cactatatga
     5101 tgacgaaaga ctatgaattt ttacgcatcg tctaccctaa gatgcaatca ttaatgagat
     5161 acttgatgga gcaaacgaat gaattagggt ttatttatgg gagagcaaaa gattggatat
     5221 ttattgattg gtctgaaatg gataaagaag gaacgcttgc tgcagaacaa gtcttattac
     5281 tgaaagccta tcattccatg atcacttgcg gagatatctt agctgaaaca tatggagaga
     5341 acgaaaatta tcatactcat ctctatcaag aaaaatatca actattgaaa caaaacctat
     5401 ttgactactt ttgggatgaa gaacttggcg cttttatcga ttgctatgaa tctgggagaa
     5461 gaaatgtgac gcgtcatgcg aatattttcg ctgttttatt cgattttgct aacaaagacc
     5521 aacagcaatc gatcttaaag aatgtcttat tgaatgacac tatcacacag attactactc
     5581 catatttcaa attctatgaa caagatgctt tatgcaaact cggacagacg aatatcgtct
     5641 accaaacgat tcttgattat tggggaggga tgttatctca tgatgcggta actttctggg
     5701 aagaatacga ccctactcaa acaggaaatg aaaagtacag tatgtatgga gatccttatg
     5761 gaaaaagctt atgccatgca tggggagcaa gcccaatcta tcttttagga cgctatttta
     5821 tcggtttgta tccaacaaag ccaggttatg aagaatttat gatacaacct aatctagatt
     5881 catttgaaac actgaattgt acgctaccta tcaaagatgg aaagatttcg cttgaaaaaa
     5941 caaaagataa gatcgtgatt acttctgata gaacaggcgg aacagtgaaa atgaatggta
     6001 aaagttatcc tcttctagca aaacagccac ttgtcttaac aagctattca aaaataaatg
     6061 cataaaacaa aaaactgctt gcgacatatc agaagacttt aaatcttctt ctgatattgt
     6121 ccaagcaatt tttattttga agtttgctta attctgttct ttagaaacaa tgtttgttct
     6181 ttttccggca aaaagaaagc tagataatac gacaagtaaa aagaaagcga tgattccaac
     6241 tgtcaaggct atttgaccgt attctcctag tttcccaagt acaagtcctg gtactagata
     6301 atcagtatcg gcaaatgtag aagaagcgat tttaagatct cctaatactg gaaggataaa
     6361 taatggcagc caactaatca ataagctatt gaaagcagaa cctaataccg cccctcttct
     6421 accgccagtc gagttgccga agacaccaga agcagctccg cagaagaaat gtcctactac
     6481 cccaggaatg ataactgtag tttgcaagcc tatcatagca aacatggata gtgttcctac
     6541 aaagaagcta acaaaaaagc caattaatac agcattaggt gcatatggaa aaacgatagg
     6601 aacatctaat gctggtttag aatcgggtac gagtttttta gcaatccctt ggaatgctgg
     6661 aacgatttct gccaacacca ttcgaacccc ttgaaggacg ataacaaacc cagctgtgaa
     6721 tgttccagct tggatcagtg caaagattac agcgttgtct ccattgctta gttctctttc
     6781 aacaaatcca ggaccagcta acaaagcaag tgcgacatat acgatcgaca tcaagagcat
     6841 gatcgataca gtagaatctc gcaaaaagct gaaactttta ggaatcttta tgtcctctgt
     6901 agaacggtct ttgtttccaa aaaactttcc gatccatcca ctagaagcat aaccgatatt
     6961 tccggtatgc cctaacgcaa cttgattgtt tccagtgatg ttcttcataa acggttgaca
     7021 aagagcaggc gtaatagtta atagtgttcc ttcgaagacc ccgcctaata agatcatcca
     7081 caaatttgca gtaaaccccg cgcttactaa aatgactgct gttagacaag aaacataaag
     7141 cattgcttga cctgtaagaa aaatgaattt atatcttgtg aatctagcaa gtaaaatatt
     7201 cacgatcatc cctactagca tgatcaaagc agtaggtgta ccgtattgtt gcaaagcaat
     7261 cgccactact gcttcattgc taggaactac tccttgagta tgcagagctg tttggaacat
     7321 cgaagcaaaa ggggttagtg aattactgat taaaccagca ccgccgatca gtactaggaa
     7381 tccgacaaat gttttgatag ttccttggat cacttctgaa aatttcttct tctgaagaac
     7441 gagaccaagc agtgcaatca aagaaacgag gatggagggc tgactgacga ttgacacaaa
     7501 caaattcaac aatttatcca ttgatagttc ctcccttgaa tcatttttat tcttttgaca
     7561 agatcttcgt tactttttct tttacttctt cgctgtcaat gatcgattgc aaaggtacga
     7621 gcttttcatc aggtatagaa gttagcgtat ctttcaaaga atgctcaatg aagaaataat
     7681 cagccatatc gctagataca cttccaacat ccgtatgact tacttcaaac tcgtccacat
     7741 ctgctccaag atctgcaagt gcttgtttga tgttcatctc taccataaaa cttgttccta
     7801 atccactttg acatactgca aaaattttca tatcattcgt ctccttttct tatgatcgct
     7861 aatatttctt cttttgttga tgcgtgtaat aaatcttgaa gattttgttt attagaaagt
     7921 agctttgtta aatttgccaa agcccgaaga tgtgcctcat tatcgatagc agctaaacag
     7981 atgaacaaac taatgggatg ggctggatta tcaagcagat ccacaggttg ttgaagtttc
     8041 agcaaagaca tccctacttc gtttacgcca tcttctggac gagcatgagg taatgcgatg
     8101 tgatcgccaa tatggataaa cgcaccatac tgctccacgc gctcgatcat agctttgata
     8161 taactttctt ctattttatt ttctgataat agcggctgtg cggcaaatgt gattgcttct
     8221 tgccagtttt ttggttgatc cgataattgg atcatttctt ctgttaataa ttcagatagc
     8281 ataggacgtt gatcctcctt cttttctaaa agtttgttca ttttacgatt taatacttga
     8341 tagagctttt cttcgtctac gccttctttt aattcgatat aaggcagtag cgccgacaac
     8401 aattcttggg cagaaggcat tgaaaatcct ggtatcagcc aatctctttg tacttgattg
     8461 atcagctgat ttttttccaa gtcagtcatg attggatgca gaacatagag agactttttc
     8521 ttatcagtaa gtgtgagagg tacagtagaa aaaataatat cgtaagaagt ttcgtcaacc
     8581 tcgtgtaagc ggtcagctga attagtctct gtaaaaaaga tcatcgggaa taattgctga
     8641 agttcacttt ttaagatcaa tgaagagcta atgccacttg gacagacgat cacggctctt
     8701 atttttctat ttctttcttc tgcgtccgct tttctgatct ctccgccaaa tagtatagtg
     8761 aaaaaaccaa tctcttcttc agggatgctc ttccctattc gtctttctaa cggaagtaat
     8821 gcttttcttg tcatttcgta gatggaagca tattgatgct taatttgatc gatcatcaca
     8881 ttaggtaaat aaaaagaata ggaaatacga aaataggcag ggaccaagtg ataaaagaca
     8941 ttcatcaata attcacgaaa cgaagcaaat tctacggcca tcaggaattc catgcgatgg
     9001 atgacttctg aagaacattc cagcaaaaaa tcaagtgcag gctcctggac atctccttca
     9061 acgatcgatg ctaagcaaag agagaaaaat ataatctccg aattttgatg ttcggaatag
     9121 ccaaggccag tcaatacttg tcgactaatg tcagtgactg caagagaagc caacagttca
     9181 gcatctttta ccagttccat ggatgttttt ccaattcgaa tggttgtcaa actcaaataa
     9241 taagctaatt catccattcg atttggtgcg aatcgaagat ctgatagtga aatcgtttta
     9301 tctagcaaat ctctaacttt ggcgtaataa tcaatggaaa gggttgcggt taaatacgtg
     9361 agcccaaatc ttccagcaag tgactgcatc aaacgagcag tgcgattttt tgcttctctt
     9421 cttaatattg cttctgaccc tagcagccta aaaccagttt tcctagtata cgacagctga
     9481 caatcttcca cttctaattc atttcttatc tttttgatat ctgacaggac tgtcccttta
     9541 gaaacctgaa gaaaatcttg aaaatgaaaa acagaaacag acgttgcagt cgaataaacc
     9601 atcaaataaa gaagatctct tcgctcttgt tcggtataca cagcttcaaa tcgagtaata
     9661 tccaaaaatt tttctgccca gtcgattgct cggaatttct ttatacggat acgggtatca
     9721 tccacatatt cggcaaaagg agttaattgt ttgatcgcct cgcttgtatt tacttcgatg
     9781 atctctgctg ctttttctag actgatttct cttggtgttt tcaggagata actaattttg
     9841 tagttcacag cgctgttcac ctcctactta tattctagta tggtagcgct tttaaatcga
     9901 ggggtgtttg gtacaactta cagttggatt ttttttacga ctgtaccgaa cgatttctat
     9961 cgaatggata cagaaaaaaa gcaggtgacc aaatgtctcc tgcttttatg aattgatttc
    10021 gtccgatcaa tgatgatcat gtttattttt tgaggtcagt tcttcaaatt tttctttgac
    10081 atcttcaacc gctttttcga tatctttttc aaagtgattg ggctcattag gctttgtttc
    10141 ctcttctaca gaatttgaac cagataaagc tgtttcttta ttttctgttt caccagagtt
    10201 tgtgccagct aatgattctt ctttgtcttc tgtttcacca gaattagttc ctgctaaagc
    10261 cacttcttct tctgttttag gggccattga acctcttaat cgtttgtgtt tttcaccgtc
    10321 ataatttgtc atgttggttc ctcctagtag ttctatactt tttcatcgta caataagaca
    10381 gtttatgcgt caaaaaacaa acccgattct tacaaatatg tcatattttt ccttctgatt
    10441 cttgaatgct tttgattcct tcttccaaaa ttttcatttg taagattgtt tcttcatctt
    10501 tgatgacttt ttctgcgcca ttaactaatg tctcgtaaat cccttcgtag acacggctgt
    10561 aatcaccaac ctcggaaatt actttttctt catgataact cccttgttca tctatatagg
    10621 tcaatacacc ataatgttcg ggcaaatcga taccaaagtc ttcatgatca ggcatgtaaa
    10681 acattttcag atgttcttct tgtcgatctt ttgtttgttt cacgaacatc cctttttttc
    10741 cgtaaacgac gaaacttgga cgttctttga tgcggaaata actggatttt atcgaaattt
    10801 tcatatttcc atagaaaaaa tccagatcga agtaatcatt cattcgtcct ttccctaaaa
    10861 gttgacgtac atcatagctg atatcatctg gttcgccgaa ataagaaaga acttgatcta
    10921 ctgtatgaca agcatgtcca tataaatagc tgttcgcagt agaaaattca tgcgtatttt
    10981 ctggtacttc cggtctgaaa taatcataat gcatttctac ttctaaaata tcccctaatt
    11041 ttccgctttc gattactttt tggactgtca agaagtcaga atcgaaccgt ctgttttggt
    11101 aacattggat aaatagacct ttttcttttg ctaatgcaaa taactcttct gcttcctttg
    11161 acgtttctgc aaaaggtttc tctactagta cattcttacc atgttcgagt gccatttttc
    11221 catattcata atgaaattga ctaggtgtag tgatgacaac taattggatc tcaggatcat
    11281 cccaaagatc agacagctga tcagtatatt cgatgtcttc gaatgttttc cacactggtt
    11341 cagatgggga aaaaattttt ttgatattga ttttgttcgt aagttttctt gaaaaaggca
    11401 aatgatagcg atttgtactc tttccattgc caagataagc gattgtaagc atgagtggtg
    11461 cctcctttta ttgatgtttt cagtatatcc tagaaaagcg cagaaagtat ttataatagc
    11521 tattctggta aaaaagttca gaaaaacact tgttatcatt cggtttggtg aaattaatgt
    11581 tcaaagtaag gataaaaatc aaaaagatag tagcggaacc aagtgtttat cagtaagata
    11641 gaatttggag gtcagatcat gcttattgaa gaaaaactaa caaaacaaga gttgtttacc
    11701 acgacagaga aacggattgt cgattacatc cgtaggaata tcgaagctgc agtctacatg
    11761 acgattgaag aattagcaaa agctacgtat acttcccatt cagctatcat tcgtttatgc
    11821 aaaaaaatgg gattcagcgg atttaaagaa ttccgtttcg agctggggcg ggaagttcat
    11881 caattgattg ctcagttcaa tcaaacgatt gatcctaatt ttcctttttc aagtgaagat
    11941 agcactcagc aaattgcaaa gaaaatggcg gatttatcga tccagtcaat aaaaaaagca
    12001 caattgcaga ttgaacatga agatttagat caaatcggta aagtattgaa caaggcacag
    12061 cgaatctttc tttttgcaaa aggtgattct caaatcacgg caaggaaatt ccagaataaa
    12121 atggtcaagt tgaataagtt tttgatcatg gctgaagaat acagtgattc ttcttggaat
    12181 gcagccaatt tgactagcga ggattgcgcc atttttatta gctacagcgg tcgcatccat
    12241 cattatgaac gaataatgac ctatttgaag catgtagagg cccctacctt gctgattacc
    12301 ggaaatcagc attcagaaat ggccaaacag gctgatatgt gtcttgttat atcgcaggag
    12361 gaatatgatt ttgctaaagt agcgactttc tcctctcaaa ttgccttcga ttacgtatta
    12421 aatacgttat tctcagtgat atattctcaa aattttgaag agaatatccg caacttgaaa
    12481 aacaaacaag aattgatgca aaaaggcctt cttaaagaat atcggaaaac ttaagaaaag
    12541 atcctcattt agtaataaga aagatagtgt ttgataggct ctctttgata tcatcataaa
    12601 aacaggctga agttttcttt ttcaaacctc agcctgtttg tttacggaac gattcgaata
    12661 gatatattat agaaatgagg aatagctaac ttctgtttct tattttgaac gattgacgaa
    12721 aaagatatat aagaaggagg aaaatcagta tacatgcagc taacaatcca tacaggaaaa
    12781 atcgtttgtt ctcggatact ttctgtactt ctttttgaac ttcttttgca tgataaggaa
    12841 cccggtgccc tctgacaagt aagcgatgac tgttgaccat ataaggtgta caagttagga
    12901 gtgtcacgaa atcctgccct ttttcgattc tcagatcttt tgtatcagtc ggttctaccg
    12961 tcttgatctg atcgacttga taagcgagtg tccgtccatt gatttcgata tagaaagaat
    13021 cacctaaagt cagtttcggt aagtctgtga acaactttgc ttgaggcagt cctcgatggc
    13081 tggaaataac ggcatgtgta ttttctcccc ccgtaggata agacgttcct tccaataatg
    13141 aggcaccttt ttctagcagc acagtattcg tttcatcaaa aattggcaaa tggacattga
    13201 tctcagggat cgtcaaaata ccgatcgtat gtttctcaaa ataggttcta tcctttttcg
    13261 caggttcttt tttcttagta aagggatctg cacctggatt tccaccttct tttgctaatt
    13321 gctggttttt ctttgtcatg ttttcttgga ttttagccat gacttcttca ttttctttga
    13381 ccgcttgttg ctgataatga gagatgatct gctgatccag ataattattc agcgcgtcgc
    13441 taacaaatgg ataagctagt atccctattc ctgagagaag caagaaaagc atcagcagat
    13501 ctagaagttt ccttttcttt ggtttcattt gtgttctcct tgtgatgact gtttgataat
    13561 aaaagaaaaa ggagcatttg cgtaatccgc tgctcctttt tccaattctt gcaaaaagac
    13621 taaatctgac tgtgcttgcg tctagcaaag tacagtccag caatcagtag aaggactact
    13681 cctgcaccga tatacacata gattccctta ccgcctgttg aaggaagtgt acctttgtgt
    13741 ttatttggta ttttttcagg agaaatcaac tgtcctgctg ttacataaga ttgttcatcg
    13801 atagtaaatg ctacacggtt tgttaatgga acatattttt ctggcgcttt cgtttcttcc
    13861 agatagtacg tgccatattt tagacctgtc acatcgatta aaccgttact tgtggttgta
    13921 aaaaccgttg ctgattcttt cgccgatacc cagctgacgg cttttgtgga tgggtcgatc
    13981 gataaatatt tcgctgtgtc actatcttga tcacgaacga cgaattctgc tccagcaagt
    14041 gtttggtctg atgtaacgtc accatctact ttaacaaatc gtttgccccc agtaacgaca
    14101 tcgactgacg gtggtgtttg atcatttgta tgcccgttat cgacatttgc ttggttagaa
    14161 aatcctttgg ttggatctgc tttttcgttc aaatgcatat agtaaacgaa tttcaatgta
    14221 ccgccaggag ttaatgaagg aatataattc ggatcaactg aaaccgtgaa tccgtctgtt
    14281 tgctcagtga cagaataatt tactgggtcg atttctttat ttccatcata taaggcataa
    14341 gcatacgttc ctgaagaatc attatcaaat gttaaagcag cgtcatgagt atcgataagt
    14401 ttgaatgttg tgtacttgtt ttgcgttcct tctttatcag cgatacctaa tgggatattg
    14461 acagaaatct catattgaat tttttcaccg attgcgacat cttttccatt caattgaggt
    14521 gttgttttat caactttaga cgtatcattt ttgatggtct tttctactgg tgtttggcta
    14581 tttgccagga tctcaaaagg cgtgattgtt tgcttttcaa tcatttccgc attatcagga
    14641 gcttttactt cttctaaatt atattttcca acttctaaat gattaacgat caactggcct
    14701 ttttcaatag atgcctcggc aaagtcattg ttgccgatgt cataagaatg accagtaatg
    14761 aaatgtttgg ctttggtttg atcagttgtc caagtgtaca atccatctgt gacactttgg
    14821 atgtattttt tgacgcttgg tgttccttct tctttagaaa taataaattc tgctccattt
    14881 agtgcttcgt tttcggcagt accgattttt gtaactttca acgttccatc attaccgact
    14941 gtatttttag ggtagagatg gatagtatct agttcttctg tcccgtattt ataagagcca
    15001 tctgcttgtt tgatcatctc atatacaggg aaagctaaaa ccatgtttgc ggcagctgac
    15061 actccgtctt ttggttcttc tttgatcgtg tagactgcat ctttcccatt ttgtttttta
    15121 ggtaaagata aagtgacatt tccatcagca tctgtcgttc ctgaagcaac tggtgtacca
    15181 ggagtcaaag actggactgc ttgttttgct gcatccacgg acgctccttt atctcgttgc
    15241 gcataaaatt cttgagtgac gttataaact gaaaatgaaa tatcggctaa tccttggtat
    15301 tgatcgaatt cgctcatttc tttcccgctg ttttggatta aaggatcggg taaatcagtc
    15361 attttctttt tatgaaggat gacttgagct gctgtctcct ctgcaaacac tttttttgcg
    15421 ccgaagctgt ttgtgagtaa tggtaaaata aggaaaagga ctcctaacat aacgtttatt
    15481 tttttatgat ttttcatttt ttcactcctg ttcttttttt gataaacaac cagattgcaa
    15541 agcttgcgaa taccatacca acaacgccta aaagtgtatt tttcgtttcg tttgtttttg
    15601 gaagaaatgg acggctattt tcttttttat tgactacttt caatatactg tccgcctctt
    15661 tatcctcaga aagaaaagtt gaaatggtaa acggaatttc tgtttcactt aaaatataac
    15721 cagaaggtgc ttcgatctct tttaatcgat aacttcctgt tttcaacccg gaaattgaaa
    15781 aagcgccatt tttatcggaa atcaggtgaa gcgcatctga ttcgttcttt gtccacttgt
    15841 atccgttggc tgtttgctgg aggtaatttc ctgctttgtt tttaataagg aatactgcgc
    15901 cagctaatcg tttatctggt ttatttgtct cgattttgac aaattgctta ctccctgtat
    15961 atacagagac tttctttgta ctgacaccaa aaccaaaatc cagtgttccg ttgttgttga
    16021 tttccttgtt cgcctgtgct gtactgctta gctgcatctg ataagaaatg gtcagttttt
    16081 ttccagcata tttttccaac gaagggatcg aaaataaaac atcaaatccg tgaggtttct
    16141 tgtgtatgac aaagccttct gtcagctttt ctccattcga tgaaatcgtt aaactattag
    16201 gtaaaaacgt taatgcttca tccgcactat ctgacaattc gaacttttga tagtcaagga
    16261 tatttacagg aattgtcgta tgcaaggaat aagtgatttt ttcaccaatc gtaaaatcat
    16321 ttctaggctc ttctaatacc ttctcaaaag gtggatcagg gtagtcgttt tcttcatttt
    16381 taggatacaa atggatagtt gaaagttttt gattgttttg tccatgtaca ggaagaacaa
    16441 ctaccatatt ctctgccttt tctttgacga cttctggtac tttggattca atgaaaagat
    16501 acgctttatc tcttttttct tgatctttag cggccagaga aaaagaagcg ataccatctt
    16561 cgttgttaag agttgtagtt gttgcttctg cggtaaacat accggaagac gcaccgtttt
    16621 ttgcgatctc tgcttgtgct tcttctaccg ttttgccctt ttcccgtaga tggtaaaaag
    16681 aatctgtgac atcataaact tggaatgtga caccatttaa tcctcgatac gtttgtaata
    16741 aagctttttc ttgtccgtca tttggatgat ttttcggcag ttgcccgttt gggaaaagca
    16801 atttgtgtaa agtaatctga accatttctg tttctgtttc tgttgctacc tgagtaaaag
    16861 ctggtttaaa tagtaacaag aagagacaca tactaagcca accaagtttt ttcatattta
    16921 gctgccccct ctttcttttt tcaaaaaaag ataccaaata gaaaacgcac aaccaatcat
    16981 ccctactaga tagattccta aacggccaat tcctcccgtt tcaggtaatg gtacttttgc
    17041 ctgattcgtg atgtctaaag aaatctggtt gtttttggct cctggtgaca gaacagattc
    17101 atgatcttgt ccatccactt gaattgaccc atcttcgtgt ataactatag taactggctc
    17161 ttttagacct tggtatcctt ctggtgtaaa agtttcggtc agcgtataag ttccaggttt
    17221 taaattttcg aatagaaagg tatctgtttc ttgtccatct tttggcgatt ctaagtccat
    17281 ttctggtccc tgcagtctga attttgctcc ttttaatgtc tgtccgttgt cttctttttt
    17341 gtgtaccgtt agatcaaacg cttttagtcg attttgatga gtgagcgtcc atacattatt
    17401 tgtcatctcg gtattttttc ccgtgtaaat gatttttcca tatttatcga tttggaattc
    17461 ataatttccc ggcgaagtat catatccggc aggaccagct gtttcaacga ctcgatattt
    17521 accaggtgtg agtgaatcaa aactggcaag acctttttca ttcgttgttt ggctgtcaat
    17581 tggctgataa gtaccatttg cttctttttt ctgtagggaa aatgctgctc ctttaagatt
    17641 tatctctttg tccgtccctt gcatcgcata cttacgtact gctacaggaa cttcaacaaa
    17701 aggattttca atagtcaact ggatcgtatc atcggaagta gtgactgttt ttccatcaat
    17761 ggttaccgta ccatcagaag cgatcttgat ttcccaagta gtctttttgc ttaatccatg
    17821 cccttctgga gcttttgttt ctgtcagcgt ataggtcatt tctttttgca atttgacatt
    17881 ttctggaaga gaataggttc cgtcgccatg atctgttagt aaagtatcaa tagaatctcc
    17941 tgttaatttg aaaacagcgc caataagatc cttttcacct gtagaggaat ttttcgttat
    18001 tttcaagttt aacggtgtga attgtttagt atttttccat gtcattgcat ctatttgttg
    18061 agaatcgtat cctggtacag gtaattcttt gattgtttgg taactgaatg cttgaccttg
    18121 attattgtat tgaggtagtg ataatatctc ttgataactt tctccgctat ttgcagataa
    18181 tttgtcaatg tctgcacgtt cccacgtatt tgttgtatct ttagctggtt taatgattcg
    18241 aatatatccg tttttccaag ctgcagcatt tgtcgtatgt tcccgttgaa tctcaaaagt
    18301 aacttgatct ggacgatcag ctagattgtt gtcaaattct tcccataact ttttgatgtg
    18361 aagactgact ccgggagctt tagcagatgg tataccgaat tcagctaatt cattggtatc
    18421 aatgtttggc tggaaagttg tcctgccgtt catttgatac cagaaatttg gatggaagtc
    18481 ctcattttct gtttggattc tcacttggta atggatttgg atttcttggt cttttcctaa
    18541 atagatctga ttgctcttga tggtatttcc ttctatggaa atagatggag tgaccgttgt
    18601 aggacttgtc cccacactct tgactgaaag tgtaccaggc tgataaatga atggttctgc
    18661 gattggatca tttatttgtc cattgcttac agttgctgag atctgtacag cttttttggc
    18721 taggtattca gagatatctg ttgcatgatc agctgattca tagtaaagat cgcctttttc
    18781 atctgatgaa accatttgtc gcatacgaga ctctacttct gcttttgaga gaccagctgc
    18841 cggatcgctt tgaagttgga tgccaagacc atgtatttcg attcctcgtt ctttgagtgc
    18901 catcgcttct ccgatggttg cgataaacgt actgtcgatt cttctggata gattgttttg
    18961 gtcaggtgca tcatagattc ttgagattag agaagtattt cccggccgat cttgcgtatt
    19021 agaaaactga gttccgtaat aattgctgct tgattgtgcg tgtacccgct gtactttata
    19081 ggaaaatgtt ggtacaccat ccgtcagcaa aacgatcact tttttatgtc catttggaac
    19141 ggatagcatg cttcctgcat ctcttagtgc tttttgtgta aaagtaccac catttgtccg
    19201 tgaaggtgta atggatttta cttgattttt cactgaatca aatgaaccca tctgtactgc
    19261 accgttactg tagctatatc cttcgcttga gtagccgaca tatcccatat tgattttgtc
    19321 tgtgataccg ctatctgcta aagtatcgac aaaacgatcg acaccaatct ttacttcacc
    19381 gatccgatta ttgtcgttca tacttcctga ccagtctacg accaatacga gatcaagagg
    19441 cgtgatatct ttttgtacat ttccacgagc attcaaataa acatcaaaca atccgggtgt
    19501 acttgtttcc tttgcatact ttcgtatcgc atagtctgct tgattaccgg ttcctccata
    19561 ttcgatatac gaatgggtcc gatcattagg atctccattc caagaattta taccatccca
    19621 ttgatttgtt ccgtttttat tgccttgatg atcaagaaca tttgtatttc ctgtaggttg
    19681 ccaactggct ttaggataag tgccctgatc atctgttgta tattctgtag gaatcgtttg
    19741 attagatggc acactcacag ctggcaagga taatgtaggc aatgtaaatg ggctgacaga
    19801 attttcgttt tcttttgatg attcttcttc tatttcgtct ggctttggta ctgacacatc
    19861 tgtatctttc ttattttctg tctcatcaat aatgtcatat tctttattat cgacgatcag
    19921 cttgtttgcc tggctgtggt aaagtgctgg atcgattgga aaactcagat cgatagtttg
    19981 tttagtgttt gatgagattt ccaacgtaat tttcccgcta tttacctgat aagcatagcc
    20041 atctccttct tttctttcca catcacttaa gtaattttcg actagaccaa tttctttttc
    20101 tatcttttca gcactggtgt tattcgcatt tagcttgatg ttcagtgctt tttttccaga
    20161 ctgatccgaa atgacttcag ctgaaattgt ttctaaaact gttggatcat ctgtagtgtc
    20221 tgctattgca gataatggca caaagtttga taaactcaat attatcagca tgaaaataac
    20281 tttcagtttc ttccctgtgg tcgtcatttt tcccctcctt tttctttttt tctgttttga
    20341 cgtgaatagt gaaaattatt aaaataaaca ggcgttacat aattttattt attttaatat
    20401 gataattatt atacgaaggg cgctaaaggg aatcacacca attttcatac caattggttt
    20461 catttatgaa gttgattcct tcgattaatg agcgtttcat tgaaagaaaa gggttgaggg
    20521 agaaaaacat atgatcaggt tgttggaagg aaactataga aagatggaaa aaatcattcg
    20581 aattctgtcc atttctgaca agtggatgac taaaaaagaa cttgccaaac gaattggtag
    20641 ttcagaatct acatttgtaa ggtatatcga agagatcaaa atccgctggg gaacggatgt
    20701 aacgattcaa tcttcacaaa aattggggta tcgtcttgaa catttgaatg taccagctta
    20761 tttaaatgtt ctgatggaaa tgtcgcaaag ttctacaaac agtcgtttat taaatgagat
    20821 tattgtcaat ccgggtaaaa cgattgagta ttattgtgaa aaaatttcca tcagccggtc
    20881 ttcttttaca cgtaagttga aacaatgcaa tcaaatttta gaaagctatt tattaaaagt
    20941 gatcgttgac caaggttttc agttaactag ccttaaaagt gagctccagt tgcgtatctt
    21001 tgttaccttt ttctttttaa gttactacgg aagacgcacg ttgccatttc caatcgaaaa
    21061 aaagaagatc aaagaaatta tggtcaagaa taattgtcag atgaatttgt ttcaggaaga
    21121 aagcccatat gagcaatctt tctttattat gtattttgtt gtgcatctca tgagagaatc
    21181 ccaaggaact ccatttgaat ggaaagctgg agactcttat gtacagttga taaatatcga
    21241 taaaaaagac ttcttaatct tgaaaaatct cttaggaaaa ttgaagtaca gcagctatat
    21301 aaaagtagtg aactattttg tcaatacgat tttttcagaa ttttcggaga aagaaaaaaa
    21361 tgaaatcagt gagcagatac gaaaaaatat gtcctactgt tcgttttcat ccgctgattg
    21421 caaagaaaaa aacgaagaat atgttgttca attattaacg caaatttatt ttttctcccg
    21481 tattttccca tacgatacga ctcatttgac gatccgtgca acttactttg cacagcagat
    21541 gcaacaccgc cagatacgtc tatatgctgg atttcgtgca gaacttcaat tttattccac
    21601 tctactgaat cagaatctga tttattattt atctagctat atcttttgga tgcttatcgg
    21661 taatcctgaa attcaccatt atacttctga taaaaaaata ctgattatca gcgacttaag
    21721 tcttcgacat agtcaatata ttgaagaata tatcagtgat attcttgctg tgcataaaat
    21781 tcactctgag acaactgcta ttactgaaga tcagctgtcg aaatacaaac ttctcgagta
    21841 tgatctgatc gttacgaatc aacccatttt gaatgcccat gttcctcata tattgattga
    21901 tgatagtgtg tcattcgcaa atgaagaaga actcatccgc ttattcgagt tatgaataat
    21961 caaaaaacag ctatgtataa accaccctga taaaaatttc aggacaaata cacatagctg
    22021 tttcttttta ttgtattcct tcagcaatcc aacgagattt atagcgaaag tcttttaaca
    22081 actcttcttt tgtccaatta tttgcaatga gatccgtcaa agtgttcatt acgagggtca
    22141 tggaaaagta taggaaacta actgcttctt ccgacgattg aatggagaaa tgattgattc
    22201 ttaataattc cagccatttt tcgttcattg gagaatcagc cgatagttct ctacgtttgg
    22261 ttgtctgctg atcatttcgc tgggttaaca agtataacct ttgccaatag atactttcgt
    22321 gatctaagtc gcagcaccac tctaaatatt tttcgatccc caaaaagaaa tgttgtttgt
    22381 tttcgctaat gaattttaag atatcttgat ggactatgtg agaactgctt ttgatcgtat
    22441 acaaataagc atcttccaaa tcttgaaaat atttataaaa agcaccgcgc gacatcccca
    22501 tcttgtcaac gatttctgag actttgactt ggctgatgtg ctgtgagcaa aatatctcca
    22561 gtaataggtc atcgattctc tgtttctttg cgttcgataa attgaaaaaa gtttcttttg
    22621 gcatccacat ctctccttta tctctgatta tacgtggata gcccttcatt gtcatgaagg
    22681 attcattcct tctttttctc ttcttctgtc aagtacccat cttccattcg ataaacgcga
    22741 tcactccatt gaatcatacg gacatcatga gtaaccataa tagtcgcttt ttgtttctca
    22801 tgagcttctt taactagtaa tttgacgact tcatatgcat gatctgtatc caaactagct
    22861 gttggttcgt ctgctaagat caagcttggc tcattatata aagcgcgagc aattgccact
    22921 ctctgtcttt ctccaccaga aaggtcttta ggataactat ttttaagtga ctggatatct
    22981 aaagattgca gcaaggaatc gatttttgtt gaatcttctt tttttgaagt aacttggtca
    23041 actagttcaa attgttcctt taccgttaaa aaaggaatca aattagagct ttggagaata
    23101 aatcctattt ctttaaagcg taaatctgct cgtttttttt ctggaagctc agttagtgag
    23161 tgaccgttga tcactatttc tccggaagtc ggtgtttgca atcctcctga aatggtaagg
    23221 aaagtacttt tacctgaacc agaaggacca atgatgctga cgaattctcc ttcctttaca
    23281 taaaaatcaa ttgctttcaa agctgagacc tttgtatgac cagaaccaaa tgttttggta
    23341 acttttttca tcgttaatat ttttttcatt tttattaacc tcctattgct gtaattgggt
    23401 ctactttggt tatcgtacgt atcgaaaaca atccgcctag cattgcgaca atgatcaaaa
    23461 tcccactata tagcaaccat tgtgaccaaa aaacagcaaa aggcatcgcg cttggtaaaa
    23521 tcagactagt caaataagca aacaaaagag caagcagaac gcctaccacc ccaacaataa
    23581 aggactgtgc tactaaagat ttagcgatga agaaattttt gatcccttgt gctttcatta
    23641 ctccaaaaat agcagtcttc tgaagtgtca tcacgtacat gaaaataccg accacggcag
    23701 caaccactaa gaacaaaaag tatatcatcg catttaaggt catattttgg gcagaatagc
    23761 caggaatact ttcaatgaat gtcccaatag ttaataactg tagacccttc gatgcttttt
    23821 ctttcgaaat tttagctgaa tcctttatta cgatcccatt gatcggctgt tcttcttcag
    23881 aagcaaacgg ctgttgtcca tacttcaaag cagtccacgt atctagggac gcatagatga
    23941 cgggagaaac tgtgtaataa gtttcaggaa agataccgac aatcgtcaaa ggatcgtcgt
    24001 aactgccaat tttgatcgta tctccaattt tatagcctga atcagccaga ttttgagaga
    24061 tgatgatttc attctttgtt ttaaattcat gccctttcgt gagttttggt aataaaaatg
    24121 cttctttcgt tgtgccgaaa actgtaacat tttctttgtt ttttcctttg attgctccgc
    24181 tgtacaatcc gattggtgct ttctctcctc cttcaatgta tttcagatct cctcttgtta
    24241 attgtgaagc tgcaaatact tgatttgcat cctccgacaa aacaatctct tgtgcgtccc
    24301 aatcatcgat tgctttttta aactcttctg aaagtccatt cgctagtcct gacaacatga
    24361 atacgacata cgcaatcaag aacataatac cgacgatcaa gccatatcgt aatttagaat
    24421 agcgaatttc tttgatagct agaaacattt gtttttcctc cctatgatag attgatagtg
    24481 acaaaatgtc acttgttgaa tagtgtaact ctaaagtgac aatttgtcac ttgtgttgtt
    24541 tttttcttat caataaattg tctaagacat ctttcgataa aaccttaaca tgagtcataa
    24601 aaaatttatt taagccttat ttatgctttc catgataaac taaagtgacc aaatcaatga
    24661 ggtgggaaaa tgattaaact attagtagta gaagacgata aagtattatc caataatata
    24721 aaagaaatac tcgctgcaat cggcgagata acacaagttt atgatggtag tgaaggactg
    24781 ttcgagttgg aaagtggagt ttatgatctt gcaatcttgg atttgatgat gccggaaatg
    24841 aatggatatg aagtgttagc aaaaatacgg aaagaaaaaa tacagactcc agtattgatt
    24901 ttgactgcaa aagatagttt ggaagataag attgaaggat ttgaacgagg tgcagatgat
    24961 tatctaacaa aacctttcta tcgagaagaa ctgatcatgc gcgtgaaagc attattgaaa
    25021 cgctcgctag gtctctttga tgaaaatgtt ctgaagtata aggatatcag ttgtgattta
    25081 aaaagcaatg aagtcttgta taaagaacaa gttttgccca tacaaggaaa agagtttgaa
    25141 ttgctcgttt attttttaca aaataaggga atcattttga caaaagagca gatatttgac
    25201 cggatctggg gatttgattc tgatacgacg attacagtta ttgaagttta tatgagcaac
    25261 ttacgaaaac atctgcgaag tactggattg gataaagaaa tcaaaacctt acgtaatgtc
    25321 ggttatatac tgcaaagggt gtgatgcaat gaatggagat attacacgaa agcaacgtag
    25381 tcgctttttt gcgacaaatg tagcagcatt tgcttttatc ttttttcttt tgggattcat
    25441 tactttacag atattaaacc aatctgcata cagagaaaca gatctgtcat tagagaaaat
    25501 aaattttgat tctcgaacag tacaattaga gatacaaaga tatcagcaag gcgatccctt
    25561 tttaaacccg actcaaacgg aacaaaacag aacaaacatg gaacagccat taatggaacc
    25621 atcaaatgac cgttttaaca ctcaagtgat cttgtggtct aaaaatggtg agatattgaa
    25681 taaagcagct attggtgggc gtttcaacca aatcaaagaa ttaaaaatgg atacgaaaaa
    25741 tctgaatacc atccaaaata ttgaattaaa tgaagacagt ggctcatctg ccctctcttt
    25801 ccgttcgatt acgaaaaaag cggagacgaa tcaagatggc gttgcctaca tccaaatttt
    25861 agaaaataca aatcaaatca agaattcatt agcgactttt cgcacgatta tgattttgtg
    25921 tatgattgtt ttttggattc tctctattgg tatcagttat tacttgtcta gtctaagcat
    25981 gcgccctatt attctttctt ggagaagaca aaaagaattt gttgagaatg cttctcatga
    26041 attgcgcaca ccgttaacga ttattcaaaa tagtttggaa catcttttta ccaagcctaa
    26101 tcatactatt attgaagaat ccgaaagcat cgctcaagca ttgtcagaga cgaggcgcct
    26161 cactggattg acttcggatt tgctgacaat cgctcggaat gattctgatc agcagctgct
    26221 ttcaaagcaa atgatcaata cacaagaata tatcgaacaa cttgttaaac catttcaaga
    26281 gatggccatc atagatggaa aagagtttat tctccataac gatgcaacat tagaagtacc
    26341 aattgatgaa aaaaaaatcc atcaagtttt ggttattttg ctggataatg cattaaaata
    26401 tacgaatcca gaagataaga tcattttgga ttcatctgtt aaccataaag aatggctgat
    26461 tcacgtaaag aatactggcc cttctatcag tgaagaagat caagcaagga tttttgatcg
    26521 attttatcgg gaagatcaat ctcgggcgaa agagactggc ggatacgggc ttggcttggc
    26581 aattgcaaaa caggtaatcg aacagcatca tggacagatt tctgtatcca attatcttcc
    26641 acaaggagtc gattttaaag tccaattgcc gttgaagcct aaaaaataat gcttatccct
    26701 aaaaaagggc tgtgacaaaa atcttgtcac agcccttttt tgttgcgaat aaacggtgtt
    26761 ccagaagtag cttcttcgga aataagccga aatttcttta agctgaccac ttctgtcaca
    26821 acctctttag cttacgctta cgatttttta ttgcggtctt caatgtgtac atcaccgctt
    26881 gtgtcaatat ccaattcttc tcggctaaca tcttctgtca ctttttcaac atcttcatgt
    26941 tcttctttat gaatcccaac ttcttcacga ataactgtat gtttgcttac atctacttgc
    27001 tcttctttga ttgggatagt aatcgtgtca tcttccaatt tttcgtctgt tccctgtgaa
    27061 gattgatctg ttactggttt gcgttcgatg acgatttctt cacgtttgac cggtacttct
    27121 accgtttcag tatcgttgac cacatgtttg tggatgtcga cttctccagt tgtcacatca
    27181 tgtgtattta catctaattt ttcttctttt agttgaattg tatcatcatc tagatttggt
    27241 ttgcgatcga tgtcttcagt cttgcttgta cccattgtat cagtctcgcc aggtacgtcc
    27301 atcggatcag ttcctggtgt catattcaat ggatccgttg tattttgaaa gcctgtcgtg
    27361 ccacctacgt ccattggatc tgttccagtg ccagtttcta atccagtccc cattgtgctt
    27421 gctgggctaa cagtatcttg tgtatccatt gtatcttctg ttgtttctgg ctgacgatag
    27481 ccttttactg tgatgacaaa ttttccatca gaaatatctc ctctatattg atataaagga
    27541 tcattttctg gtgtagccgt ctcagaatca taggtatcaa aagaaaaagt atcttttatt
    27601 ttttcccata aagaacggtc ttcttcacca tccatccgat tttcatctgt ttcaacatct
    27661 ataccagaca atccgtcaaa tcctagtcta gatgctgctt catcagtagt ataaataacg
    27721 atatcatctc gttcataccc ttcgttacgc aaccgttcaa ttactgataa tgcttcttcc
    27781 ctcgtactat agcttccgat gaccgttaaa tttttctctt ccatgtaaat tcctcctagc
    27841 aaatagattt atgattggta tattgttact tataaattta ttctatagga gacgattgtt
    27901 ttctacaaat atttagattg gaatttgatt aaaacaattt aattgaaatt tgaaaacatt
    27961 tatgttaatg aaagtcatat tctaataaaa tgtcatttgt ttctatatga atttttatga
    28021 aaaatttcat ataatttcca ctcgttgtga tggtttaatt ta
//
LOCUS       DACDIG010000031        27960 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.031, whole genome shotgun sequence.
ACCESSION   DACDIG010000031 DACDIG010000000
VERSION     DACDIG010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27960
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.031"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            113..601
                     /locus_tag="IV247_001440"
     CDS             113..601
                     /locus_tag="IV247_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777517.1"
                     /translation="MELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTAAEL
                     QKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSIQFP
                     QLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVAMEK
                     AN"
     gene            660..1100
                     /locus_tag="IV247_001441"
     CDS             660..1100
                     /locus_tag="IV247_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777518.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1112..1780
                     /locus_tag="IV247_001442"
     CDS             1112..1780
                     /locus_tag="IV247_001442"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP8777519.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1844..2467
                     /locus_tag="IV247_001443"
     CDS             1844..2467
                     /locus_tag="IV247_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAP8777520.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2523..2909
                     /locus_tag="IV247_001444"
     CDS             2523..2909
                     /locus_tag="IV247_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAP8777521.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3122..4513
                     /locus_tag="IV247_001445"
     CDS             3122..4513
                     /locus_tag="IV247_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAP8777522.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            5124..5492
                     /locus_tag="IV247_001446"
     CDS             5124..5492
                     /locus_tag="IV247_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP8777523.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            5672..6613
                     /locus_tag="IV247_001447"
     CDS             5672..6613
                     /locus_tag="IV247_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAP8777524.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            6682..7413
                     /locus_tag="IV247_001448"
     CDS             6682..7413
                     /locus_tag="IV247_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAP8777525.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            7557..9737
                     /locus_tag="IV247_001449"
     CDS             7557..9737
                     /locus_tag="IV247_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAP8777526.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            9757..10239
                     /locus_tag="IV247_001450"
     CDS             9757..10239
                     /locus_tag="IV247_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAP8777527.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            10255..11016
                     /locus_tag="IV247_001451"
     CDS             10255..11016
                     /locus_tag="IV247_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP8777528.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            11147..12481
                     /locus_tag="IV247_001452"
     CDS             11147..12481
                     /locus_tag="IV247_001452"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAP8777529.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            12788..13273
                     /locus_tag="IV247_001453"
     CDS             12788..13273
                     /locus_tag="IV247_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAP8777530.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            13266..13949
                     /locus_tag="IV247_001454"
     CDS             13266..13949
                     /locus_tag="IV247_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAP8777531.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            14075..16720
                     /gene="polA"
                     /locus_tag="IV247_001455"
     CDS             14075..16720
                     /gene="polA"
                     /locus_tag="IV247_001455"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAP8777532.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            16765..17601
                     /gene="mutM"
                     /locus_tag="IV247_001456"
     CDS             16765..17601
                     /gene="mutM"
                     /locus_tag="IV247_001456"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAP8777533.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            17565..18194
                     /locus_tag="IV247_001457"
     CDS             17565..18194
                     /locus_tag="IV247_001457"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAP8777534.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            18246..18578
                     /locus_tag="IV247_001458"
     CDS             18246..18578
                     /locus_tag="IV247_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP8777535.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            18890..19381
                     /gene="nrdR"
                     /locus_tag="IV247_001459"
     CDS             18890..19381
                     /gene="nrdR"
                     /locus_tag="IV247_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAP8777536.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            19404..20777
                     /locus_tag="IV247_001460"
     CDS             19404..20777
                     /locus_tag="IV247_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAP8777537.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            20777..21703
                     /gene="dnaI"
                     /locus_tag="IV247_001461"
     CDS             20777..21703
                     /gene="dnaI"
                     /locus_tag="IV247_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAP8777538.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            21832..22272
                     /locus_tag="IV247_001462"
     CDS             21832..22272
                     /locus_tag="IV247_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP8777539.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            22416..23117
                     /locus_tag="IV247_001463"
     CDS             22416..23117
                     /locus_tag="IV247_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAP8777540.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            23114..24235
                     /locus_tag="IV247_001464"
     CDS             23114..24235
                     /locus_tag="IV247_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAP8777541.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            24250..25482
                     /gene="pepT"
                     /locus_tag="IV247_001465"
     CDS             24250..25482
                     /gene="pepT"
                     /locus_tag="IV247_001465"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAP8777542.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            25614..26522
                     /locus_tag="IV247_001466"
     CDS             25614..26522
                     /locus_tag="IV247_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAP8777543.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            26557..26829
                     /locus_tag="IV247_001467"
     CDS             26557..26829
                     /locus_tag="IV247_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAP8777544.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            26840..27886
                     /locus_tag="IV247_001468"
     CDS             26840..27886
                     /locus_tag="IV247_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAP8777545.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
BASE COUNT         9398 a         4837 c         6094 g         7631 t
ORIGIN      
        1 aaataaaaca ttgctgcatt tttcaccaga gttccatcta gaggggctaa cattaggaga
       61 aatctatcaa gcagaagggc tgtcggctgt tttgcacttt tttaaggtag aattggaatt
      121 accagtaaaa gattatatcg agctttctct tgaatcttgg gatcgagcag tggttgaact
      181 gtttccagaa ggtttaatga tcgatacaaa cgatgggaag atccatctga ctgcagcaga
      241 actgcaaaaa caaatgcgtt atcaaataga tgacgaaaat tctttttcta tttttagacg
      301 tcagcaaaag attctaaaaa gctttttaaa agtgatcagt aaaaaacgga atctgatcaa
      361 aacgacacag ctgttgaaaa aatatccagg tctgcttcac acgtctattc aatttcctca
      421 attgattacg atgatccatc gttttatacg tatgcaacag ctaccatcta agaagctgtt
      481 tcttccattg acggacacat ttcgagtagt caacagagaa gataaaaaga aagtgattgt
      541 gattgacttt accagaaatc ggaatgtcct tcaccaagtt gcaatggaaa aggccaatta
      601 aggctttttc tttttggatt tttttgtcat aataaatatt agatgatgga ggagataata
      661 tgatgcaaac atggttagaa aggctaatac gatttcagca gaactatcac aaacttatgc
      721 agggaagata tgcgaaattt gatcagttga atcgcggtct attgatcgct gcgcttgtct
      781 ttagtttgct aagtcgttgg ttgccctata gaatcggaca attgttgttc atcgtctttt
      841 tcggatggat gatatttcgt ttcctatcaa aaaaaatcta ccccagacta aatgagaatc
      901 aacgttattt gaaatatctt gatagagtca aagaaaaata tcatcagata aaaaacaaaa
      961 agcaagtcaa aatggatcga aaaagttata ctttttttga gtgcccgaat tgtcatcaaa
     1021 agcagcgtgc acctaaagga aaaggacgaa tacgtgtgac atgcaaaaca tgtggtataa
     1081 aattcgaaac gaatgtttaa gggagaaaag aatgaaatta gtattattac gtcatggcga
     1141 aagcgaagca aattttgaaa attactggac aggatggcta gatgtcgcct taacagaaaa
     1201 aggaaaagaa caagcagcag ttgccggtca aaaaatgctt gaagccggac ttcaattcga
     1261 tgccgtttat acctctgttt tgaaacgtgc gattcttacc ggacaagtcg cactagaaga
     1321 aatgggtcag ttatggcttc ctataataaa aacatggcgt ctaaatgaac ggcattatgg
     1381 ggatcttgtg ggtaaaaaca aagataagat gaaagaaata tacggaaaag atcaagtaaa
     1441 aacatggcgc agaggatata cggaagttcc gccgttaacc gcagaaaata gatttgaccg
     1501 cagatatgat cacttggatc cgcatttgat cccatacgga gaaagtctgg aaatgacagt
     1561 gaatcggatc attcccttat ggcaagatca tctagcacca aaactaaaag atcggcaaga
     1621 tttactagtt gtgggtcatg gtaatagcat ccgagcactg acaaaatatt tagaagatgt
     1681 tcctgaagat caaatggata cgatcgatat cccaaatgca caaccaatcc agtatacatt
     1741 agcagatgat ttgacaattc tggacaaaaa aatactttaa agaacacctt tatctaaaag
     1801 tgagagatag aaaaaagaag caaaggaatg gaacaaaagg aacatgaaaa attatatatg
     1861 ggactttgat ggtacattgt ttgatacgta tcccgcaatg gtagatggtg catggcaagc
     1921 actaaaagat tttggtatca gtatggacaa aaaagaaatc tacttcaaaa tgaaaaagta
     1981 ttctactagt tatttgatca atgaatcaaa cttgaatgcc agagagttta atgaattatt
     2041 tcaccgatat gaaaaagaaa gtacggaagt ttcccgtcca tttccagaaa cgaaacaagt
     2101 actggaaatg ctaaaagaca acggcggtcg tcatttcatc ctgacccacc gtttgacaga
     2161 atcaacatgg ggcctgttaa aagaacatcg attggcacat ctgattgagg aagtagtagg
     2221 gatcgatcag gactttcctc gtaaacctga tccagcatct ttaaattatt tgatcgatac
     2281 ttttcattta gaaagaacag acacgatgat gatcggcgat cgtcgtttag atatcgaagc
     2341 agggaaaaat gcaggagtcg ctacttgtct ttacgacatc gatcattttc tgggtgagat
     2401 cccggcagat tatgtggtag gaaatctgaa tgaaatcctt accttgcaga gcatcattaa
     2461 aaagtagtat cccctttcaa aggagatatg ttataatttt cataaaggag tgataaaatt
     2521 atatggaaat cagaagacaa aaagaagttg tagaagcatt tcattatgat atgagattgc
     2581 cgaaccaaga agtagaaaca gatttgaaag taggcttctc gcctttaaaa gaaacgatcg
     2641 aaaactatcc gaaagaacat agcatcgtag gagcacgctt agagttccgt ttagtgtttg
     2701 atacatatgt tttatctgga agtgtgagtc agatcaatca tatcctcaat cggaaaattg
     2761 aaggtcaaaa tgatgtcaca caagaagaag tggacgagct agtagcaccg ttattcgata
     2821 tcgtacgccg aatgtcttat gaggtgacag agatcgcctt agatcagcct ggggtaaaat
     2881 tgaattttca atcagaaaaa caagaataga cagtaaaaac atacctgcga tagattaaag
     2941 ctatcacagg tatgtttttt tattactaat tgtaagcaaa aagaatgtca tttcgataaa
     3001 aatcgtctaa aatataaaag tcactgaaaa aaattttttc aggaggatgg ttgaagaatc
     3061 attctcggga acctttactt ccttcgatgg acttgtcatt aataatatga ggaggagaaa
     3121 aatgacaaca ctcatcacag ttttagtggt tctagttttt gcggcattat tatatgtgtt
     3181 ctatcgtatg caaaagcgcc atacaaaatt ttcaacacgc gtctttacag cattaggagt
     3241 tggtatcgtc tttggcggtc tgcttcaatt gatctttggt gtagacgaca aagtcgttac
     3301 tcaatcaatg gaatggatcg ggattgtagg tcaaggatat gtatcattct tgcaaatgct
     3361 agttatgcct ttagtgttta tttcgatcat tggtgcattc acaaaaatga aagtttctga
     3421 aaagctaggg aaaattagtg cgactgtatt gacttcttta cttggaacaa cagctattgc
     3481 agcattgatc ggaattggtt caacaatgct gttcggactc caaggagcta aattcacaca
     3541 aggagctgca gaaacagcac gaatctctga actggcaaca cgtcaggaaa cagttgaaaa
     3601 cttatcgatt ccacagcaga ttttatcctt tatccctaaa aacgtttttg ctgatttagc
     3661 aggtagtcgt gcaacaagta cgattggagt cgttattttc gcggcatttg tcggaatcgc
     3721 ttatctaggc gtgaaaagaa aagcgccaaa agaagcagca ttcttttcag atttagtcga
     3781 cagtctgtat aaaatcatta tgcggatcgt gacgcttgtt ttgcgtttga caccttatgg
     3841 agtatttgct ttgatgacaa acgttttggc aacaagtgat tttgcagcta tcgtcaattt
     3901 aggtaaattt gtcgttgctt cttacgtagc attgcttgct gttttgattg tccactcact
     3961 gattttgatt ggtgcaaaag tcaatccaat caactatttc aaaaaagcct ttccggtatt
     4021 gagctttgcg tttacttcac gttcaagtgc tggagctttg ccattgaata tcgagactca
     4081 gacaaaagct ttaggagtag atgatgcaac agctaatttc tcagcaagtt ttggtttgtc
     4141 tattggacaa aacggctgtg ctggcgttta tccagctatg ttggcaacga tcgtcgcacc
     4201 agcagctgga gtgaatgtct ttagtttgga atttattttg atgcttgtgg caattgtaac
     4261 aatcagttct tttggtgtag ctggagtcgg tgggggcgca acatttgctt cactgatcgt
     4321 tttaggggca atgaacttgc cagtagcaat cgttgggtta gttatctctg ttgaaccatt
     4381 aatcgatatg gcacgtacag cagtaaacgt cagcggtagc atggtagctg gtatcgtgac
     4441 aagtgcacgc atcaaagatt tagatcgcga tgtcatcaat gatgattcta aagtgatcga
     4501 tgtgaatgca tagtcaaaaa gaggttgtga tagaagtggt cagctttaag aaataagaag
     4561 aagtttccta aaaatagttt ctcaaatttt tgtgaaattt cggcttattt ccgagaagct
     4621 acttctgaaa caccgtgaat aagaggttgt gaaaaagctg ccctgcatca agtaataaga
     4681 acagccaaac aaaaatagtt cctcatattt tacgtttggt tgagcttatt accgcagatg
     4741 caagcttttg aacaccgttt aagaaagagg tcgtgagcct gccgtttcac tctgagtcac
     4801 aaagagcaat ttcaaaaaat agcttctcat gtttgttgaa tattgtgtct tgtgaccgag
     4861 gagttgtcag gagaacaccg tttaccagag gttgtgactt gtttttagtc acgacctctt
     4921 tttttattta ttccatataa atacacatta tttattccga aaagtgaaag cgcaaataac
     4981 aaaaacaacc aacttattta ttattaaaga aaagcgtatt taagtaggac gattctgttg
     5041 agagctttcg tagattttcc cattttccta tggtaaactg acaaagttga gaatgttaga
     5101 acagatcgtt taggaggaaa cagatgttta cgaatgaaat aaaaatcatg ctttatgtgg
     5161 acgatgtaga aaaaaatgca acgttttgga gagcagttgg ctttgcggaa aaagatcgtc
     5221 aagaaatgga tgggacgctt ataattgaag taggagttga ggacagtcaa acctcatttc
     5281 tgttatatga tcgagcattt gttgagtccc attcacctgg cacagctact agtgccccat
     5341 ctttgatgtt ttacagtaaa gatatctttg gactatataa aaaaatgcag acgcaatcag
     5401 tcacattagg tgatatggta cagcttggag aagaatatgt ctttaacttt gctgatcccg
     5461 atggcaacta ctttgcggtt acaggtaaat aatagaaata aagactttgt ttttctgtgc
     5521 gaaacaggaa agcgaagtct ttttaattca aataataata ttatgtaaac aaaaaacttt
     5581 ccatgcgtaa ttatacacaa gcaaataaat cgtcaaatgc gaacgccttc tttatactgt
     5641 ttaggaacga aaagaaggaa ggaagaaata catggattat caagtactgc tttattacaa
     5701 ttatacaaaa atcgaagaac cagaggaatt tgccaaaaat catttagcct tttgcaaatc
     5761 tttggatctt aaaggtcgta ttctagtagc tgaagaagga ataaatggta ctgtgtctgg
     5821 aaccgttgaa aacacgactg cctatatgaa agcaatgaaa gctgatcctc gatttgctga
     5881 tacgatgttc aagatcgatc caagcgaagg acatgccttt aagaagatgt ttgtaagata
     5941 ccgtccagag cttgtaagtt taaagctaga agaggacatt gatccaaacc aagtgacagg
     6001 tgcctatctt tcaccgcaag aatttaaaga agctatttta gatgaacaga cagtggtgat
     6061 tgatgcacgg aatgattatg aatatgactt gggacatttt cgaggggctg tacgcccaga
     6121 tatacgcagt tttcgagagc ttccagactg gatccgcgag cacaaagaag aatttatgga
     6181 aaaacgggta gtgacctact gtactggtgg cattcgttgc gagaagtttt ctggttggct
     6241 tgtccgtgaa ggattcaaag atgtcggaca gctgcatggc ggtattgcta cttatggtaa
     6301 ggatcctgaa gtccaaggag aattatggga cggcaagatg tatgtgtttg atgaacggat
     6361 cgctgtcgat atcaaccaag tagataaaca aatcgtcggt cgcgactggt ttgacggaac
     6421 accatgtgaa cgatatatca attgtagcaa tcctgagtgc aatcgtcaga ttcttgcttc
     6481 cattgaaaat gaagaaaaac atttaggagc atgtagcata gagtgtgcga aacatccgcg
     6541 caatcgctat gttcaagacc atggaatctc tccagaagaa gcagaactgc ggtttacagc
     6601 tttaattaaa tagaaaacaa tagttttgat tgtcgaccat ttccaatatg cgctatacta
     6661 gcgttgagga tggtgatcca catgaaatca ttgatttatg ctattgggga tgtccatggc
     6721 aaacatgagc tttttgaaaa acttattcac tattatgatc caaagatcca tcagcttgtt
     6781 ttattagggg atctcaatga tcgaggtcct cgtacaaaag aatgtttttt attaggaaag
     6841 caattagtcg aagaaacagg agccatttac ctccggggaa accatgaaga atattttttg
     6901 caatttctgc aatcaccgga ggactggtat gcgccttata tgagaaatgg tggaaaagaa
     6961 acgtttgaaa gcctgattca cccaggggca tcggaggaat actccccaac tgaaatctcg
     7021 atgatgatcc aatcgagata ttcagaactg attaaatttt tagtccagcg ccccttgtat
     7081 tatgaatggg aaaattatct ttttgtccat gccggtgtgg atttgacaaa gaaagactgg
     7141 cgtaagacaa gtctaagaga ctttatttgg ataagggaac catttcatga agggaaaaac
     7201 aatacaggta agacaattgt atttggacat acgattacac caatgctcca tggagatatg
     7261 cagacaacag acctctggat tcaggatcat aaaatcggaa tggacggtgg ggctgtattc
     7321 ggagggtcat tgcatggggt tgtttttgac aaggaaggta ttcttcaaga tattgaatat
     7381 cagaacaacg aaggaccttg gcatggaatc tgactaatgg aaagaatgaa ggagctgtga
     7441 ctttgttgcg gctcttttta tttttataga gaatattgtt tctttcagta gtttaaccca
     7501 ggataaaaag tatgatagaa taaagaagca atttttagca gaaaagaggc attttaatga
     7561 cggaaaattt gaaccagacg atcattcagc ttgtacaaaa agaactttca gactatcgtc
     7621 ctaagcagtt gactactgta cttaatttgt taaatgaagg aaacactgtt ccttttatcg
     7681 cgcgttaccg aaaagaaatg actggaagtc ttgatgaagt ccagattcgt gaaatcgaag
     7741 agcggtatgc ctatcttgaa aacttagaaa aacgtaaaac tgaagtgatt cggctaatcg
     7801 atgaacaagg gaaactgact cccgagttag aagcagaaat cacccaagcc gtgaaaatgc
     7861 aacaagtgga agatttgtat cgtccataca aacaaaaacg acggacaaaa gcaacgattg
     7921 ctaaagaaaa aggacttgaa ccactcgcga agtggctgat gcagcttaca gatggagagg
     7981 tgcaatctga agcagaaaaa tatattgaca aagaaaaaga agtctcttct gccgaagaag
     8041 cgttgcacgg tgctcatgag attattgctg aacaagtcag cgataatgca aaatttcgga
     8101 cttggattcg gtcttatacg tataacaaag gtatgtatgt cagcaatgtc aaggatgagc
     8161 aagcagatga aaaaggcgta tatgaaatgt attatgactt tgcagaaccc gtccataaaa
     8221 tggtttctca tcgaatatta gctactaatc gtggagaaaa agaagatatt ctgaaagtat
     8281 tctttcaagt agatgaagca gcaattttag cttacttgga tcgccaaata gtcaaaaatc
     8341 ctgcctctcc ttcttcttca ttcgtcagag aagcctatca ggatagttat aaacgcttta
     8401 ttcagccagc aatcgagcga gaactgcgca atgaattgac tgaaaaggca gatgagcaag
     8461 caatcgctat tttcggcgaa aacctccgta atcttttgtt gcaaccgcca ctaaaaggta
     8521 aagttgtcct gggatttgat ccggcttatc gtactggatg taaactagca gtcgtagatg
     8581 cgacagggaa agttttagca atcgaagtta tttatcctca taaacctgca gcgcaagcaa
     8641 aaagagaagc tgctggacca gcttttatcc aattaattaa taaatatcaa gtagatatgg
     8701 tagctatcgg aaatggtaca gccagtcggg aatctgaact ttttgtggct gagcagttga
     8761 aatccgccga ccataaaacc tattacgcta tcgtcaacga agcaggcgct tctgtttact
     8821 ccgctagcga aattgcacga aaagaatttc cgcatctaca agttgaagaa agaagtgctg
     8881 tttctattgc ccgtcgcctg caagatcctt tagctgaatt agtcaaaatc gatcctaaag
     8941 ctgtcggagt aggccagtac caacatgacg tttctcaaaa acgtttggct gaacaattgg
     9001 attttgttgt ggaaacagcg gttaaccaag taggtgtgga tgtgaataca gcaagcccgc
     9061 agctcttgca gcatatatca ggattgaata aaacaactgc ccaaaacatt gtcatttatc
     9121 gtgaagaaaa cggagaattc accgcacgaa ctcaattgaa aaaagtgcca cgcttaggac
     9181 caaaagcata tgaacaagca atcggattct tgcgagtccc tggtgggaaa aacattttgg
     9241 acaacacagg tatccatccc gaaagttatt ctattgcaaa agaaatatta acttcagtcc
     9301 aactttcaga aaaagagctt ggtacagaag aagcagtaga aaaattgaca cgattatccg
     9361 tagagaaact ggctgaaagc ctatcagtcg gagaagaaac gttagccgat attttagctg
     9421 ggctgacaca acctggacga gatatgcgtg atgagatgcc agcgccctta ttacgtacag
     9481 atgtattgag tatggaagac ttgaaaccag ggatggaatt gactggaacg gtccgtaatg
     9541 tcattgactt cggcgcattt gttgatatcg gtgtgaagca agacggactt gtccatatat
     9601 cgaaactcag caaaaaattc gtcaagcatc ctacagatgt cgtgtcagta ggagatatcg
     9661 taacagtttg gatagaacaa gtcgatacga aaaaaggcag aatcagtttg acgatgcttt
     9721 ccccttatga agagtaacaa tgaggagtaa caaaaattga cacaagaaga actgcagcag
     9781 ttagtagaag agatatcatt aaaaagcttc cagcttcctt tcaagcacca agcaatgttc
     9841 aatccacgct tacgtacaac tggcgggcgc tatcatttaa acgatcatca tttagatttt
     9901 aatgcgaagc tatttgaaga gtcagacgaa gagacgattg caggaatcat caaacatgaa
     9961 ctttgccatt atcatcttca tctagctgga agagggtatc agcataaaga tatggatttc
    10021 aagtatcttt tgaaaaaaac aggagggttg cgctatgcac cagccttgac gaagaaacag
    10081 ataaagatgg agtattatcg atgccaagat tgttctgcca cgatctgccg caagagaaga
    10141 atcgatgtac gcaaatatcg ttgtggaaga tgtcatggag aaatcaaatg gattgagaca
    10201 aaaagagaag actttaatga agcacaaaaa atcagatgaa aggatagaag gcctttggaa
    10261 aaaagtgagc agaggaatct aaaagactgg aatcagctat taaatttttt agccgcacct
    10321 gaagagggaa attttgagga actgacggca cctacactta ttttagctgg aaactgtctt
    10381 cctatcttag ctgataaagc agcacaaatg tatttaaatg gccaagtaga tcaattgttt
    10441 ttggtcggag gggtggggca tgcgactaga atcctttatg agaattttga aaagcaagga
    10501 tttcattttg aagatggcat gagtgaaagt gaaatctgtc gtcagtatct aaaagaagtc
    10561 tatgatttgc ccgataaggc ttttctgata gaatcgaagt ctacaaattc tggagagaac
    10621 gccacttttt cactggagat tcttcattcc ttaggagctg taccagaaaa aatcttattg
    10681 atgaatgatc cgacactgca acgcaggact cgagcaactt ttgagaaagt atggcaaaat
    10741 gaacaaacta ttttcgcgaa tgctgtgcca tttgtgccgg aaatccttca cttttctgaa
    10801 gagatcatct ttacagcaaa agaactgaat catcaatggc ctaaggaata tttccatgcg
    10861 ctagtattag gagaaatgga acgtctccat gacgatgaga acggctatgg tccgaaagga
    10921 aaagatttta ttcctcacat tgatatatca gaggaagttt ggtcttctta caccagaatt
    10981 aaagagtcaa taaaaacgga tttttctcgt acttgaccat tttagcaagc gaatcctcct
    11041 ttccaaataa atttggttga aattttcaat cgtttataag ataatgagga gcgtgttgaa
    11101 tgtaatagct aaaagaatgg acgctagata agagaagagg attcaaatgg aaaatcaaaa
    11161 taaattgtat cattttgttg gtatcaaagg ttcaggaatg agctccttgg ctcttgtttt
    11221 acatgaacaa ggtctaaatg tccaaggatc agacattgaa aaatatttct ttacacaaag
    11281 agatttagaa aaagcaaata tcacgattct gccatttaac gcagataatg taaaaccagg
    11341 gatgacgatc attgcaggaa atgcatttcc tgattcacat gaagaaatcc agcgagcaaa
    11401 agaattaggt ttggaagtga ttcgctatca cgactttatt ggtcatttta tccaaaatta
    11461 tacgagtatc gctgtaacag gctctcacgg aaaaacaagt acgactggac tgctctctca
    11521 tgtattgagt ggtgtccgtc caacaagtta tctaattgga gatggaacag gacatggtga
    11581 tccgcaagcg gaattctttt catttgaggc ctgtgaatat cgccgtcatt tccttgccta
    11641 ttcaccagac tatgcaatca tgaccaatat cgattttgat catccggatt attatacaag
    11701 tatcgacgat gtatttacag ctttccaaac aatggctgga caagtgaaaa aagctatttt
    11761 tgcgtatggt gacgatgctt atttacgcaa attaaaagca aatgtgccga tttattatta
    11821 tggtgtcaca gaaaacgatg atatccaagc acgaaacatc gaacgcacaa cttctggttc
    11881 tgcttttgat gtctatcatg gggatgaatt tgttgggcac ttcactgtac cagcattcgg
    11941 taaacacaat atcttaaatg ctttaggtgt tattgcagta gcttatttcg aaaaactgga
    12001 tctgaaagaa gttgcagaag aaatgctgac tttcccagga gtaaaacgtc gattcagtga
    12061 aaagatcgta gcagacatga ccgtagtcga tgattatgcg catcaccctg cagagatcaa
    12121 agcaacgatt gatggcgcac gccaaaaata tccagataaa gaaataattg cagttttcca
    12181 accccatacg tttacgcgta cgattgcatt gatggatgaa tttgcagaag cacttgattt
    12241 agctgataaa gtttacttat gcgatatttt tggttcggct cgggaagaac aaggaaacgt
    12301 taagattgaa gaccttggag cgaaaatcaa aaaaggtgga gaagtcatca aagaaaataa
    12361 tgtatctcca ttgttagatt atcatgatgc agtagtaatc ttcatgggtg ctggagatgt
    12421 tcaaaaattt gaacaagcgt atgaaaaact tttaagcagc acgacgaaaa atgtactgta
    12481 atacaattac tgcagtgtcc ttataaaaat agaaagaggg ggagtggcaa aaatcttgcc
    12541 aagccccctt ttttgcaact tatgataaac ggtgttcaaa agcttgcatc tgcggtaata
    12601 agcccaatca aatcaaaaac atgagaagct gttttttctt ggctgttctt attatttgat
    12661 gcaggacagc tttttcacga cctctgtaaa ctataggaat ctgttaaatc aagggagcag
    12721 aacttgcctt ttacgctact tttggtacaa taatgtatga ttaaaaataa actggagggt
    12781 ttctatcttg aacataggta agccgagaaa actaggaaaa acaataaatg aaatcgagga
    12841 aggcgactcg ttatccttaa cagaatctat tgaagataac cagcttttgc tgtatttggg
    12901 attgacgaat gatgcaaatc ctttgtatat ccaacatgac tttgcacaaa aaacagaata
    12961 tcaacagcca atcgttccat cagtgatgct gatggggatc atcacaagtg caatttcaaa
    13021 gcatcttcct ggaccaggat cacatgttgt caatttttct gtcaatttta ttgaaccggt
    13081 tttccattat gaaacactga cttttcagtt tgaggtcata aaagtagata agatgaagga
    13141 tgtggtgacg atttctgtag aagcggagaa tacgaacgaa gatcgtgtgt tggatgctgt
    13201 cgttatggtc caaccgccac aagttcctga attggaaata gcaatcgaaa gtgaaggtga
    13261 attgaatgaa taatccaagt acatcaatgt caggtatcaa tcgtttttat gcaaaagttt
    13321 atggattttt aggtatggga cttgccctta gtgcagtcac ttcttacttg gctctttggg
    13381 tatttccttt gcaattagca acttttatca ataattttcc actaggcttt gtgggtctgt
    13441 ggatcgttga actgatttta gtaatggttc taggcgtaaa agcacaaaag aatccgagct
    13501 tagcaatcgg tggatttatt gcctactctc ttttgaatgg cataacatta gcggttactc
    13561 ttgcatacta cgatattgga acagtaacgc aagctttctt gacagctact ttgatgttca
    13621 tggggatgtc tgttgtcgga gtaacaacaa aaagagatct tagcggggtt ggaagagcag
    13681 gacaggcagc tttaatcgga gtgatcattg cactagtctt gaacatcttc attttgagaa
    13741 gtagcgtagt tgagctgttc atctccctac tgttagtcgt catctttgca gggattacag
    13801 cttatgataa ccaaagcatc cgtaatatct atgtccagac aaacggaaca gcacatagtg
    13861 gaattgctgt attcatggca ttgaagctgt atttggactt tatcaacttg ttcttggcgt
    13921 ttctgagaat ctttggacgg aacaactaaa aaagaaaaaa attcatcagt tggggctata
    13981 ccgaattatt tggtatggtc ccgctttttc ttttcgcatt ccgcaggatt ctttgttaga
    14041 atgaataaga actttacagt aaggagttaa gaagatgaca aaaaataaat tattactcgt
    14101 tgatgggaac agtgtggctt ttcgcgcatt ctttgcgctt cacaattctc ttgaacgttt
    14161 caaaaataga aacggtcttc atacaaatgc aatctatgca ttcaatacga tgtttgaaaa
    14221 tgttatgcaa aaagagcaac cgactcatgt tctggttgcg ttcgatgcag gaaaaacgac
    14281 ttttcggaca gaaatgtatg cggagtacaa aggtggaaga tccaaaacac ctggcgaatt
    14341 caaagaacaa atgccatata tacgtgattt gttgacagga ctaggggtac agtattacga
    14401 attgcccaat tatgaagcag atgatattat cggtactttg gcagaaaaag tagacaaaga
    14461 tcaatttgat gtcgtcgtgc tttcaggtga tcgggatttg actcaattag ctagtaaaga
    14521 agtaaaagtg gatatcactg taaaaggtgt cagcgatatc gaaagctata caccagaaca
    14581 tgtagcggaa aaatatgatg ggttgacgcc aaaacagatt attgacatga agggactagc
    14641 cggagatgcc tctgacaata tccccggtgt gactaaaatc ggtgaaaaaa cagctatcaa
    14701 gctattgaaa caatatggct cagtcgaagg aatttatgaa catatcgatg aaatgaagca
    14761 aagcaaaatg aaagaaaatc tgataaatga caaagagcag gctttcttat cgaaaaaatt
    14821 ggcgaccatc aatacttcag cgcctgtgga tgtctctatc gactcattga agtatgaagg
    14881 gaaaaatctc gataaactcg ttccgtttta taaagaaatg gatttcaatc agttcctttc
    14941 taaattgaat atcgtagaag aagaagtaaa aatggatgat attttgtttg aagtcgttca
    15001 tgaattcaaa gaggaaatgt ttactacgga tatggcgttg tacgtggaaa tgatgggcga
    15061 caattatcat acagaggaaa tcgtcggagt agcgtggggg acagaaaaga aaatttatgt
    15121 cacgaacgat ctatctgtat tcgaaaaccg agcgttccat tcttggatca ctgatccgac
    15181 tcggctgaaa aaagtatatg atgcgaaacg aacttatgtt gctttaaacc gctatactgg
    15241 aaaaacgaaa ggaatcgatt ttgatgtctt gctaggtgca taccttctcg atacgaatga
    15301 aaaaagcaca gatatcgaag gaatagctgc tcattacgga tacaacgata tccaatcaga
    15361 tgaagctgta tacggcaaag gggcgaaaaa aggtttacca gaagaagagg aactcttttt
    15421 tgctcattta gcgcgaaaag tagctgcaat caatgcgttg acaccaaaat tgagccaaga
    15481 actggcagaa aaaaaccaag aagatctttt ccggaaaatg gaactgccat tgtctatcat
    15541 tttggcagag atggaaataa gcgggatcaa agtagatgcg actcgcttac aagaaatgaa
    15601 gggcgagttc tctgctcgct taagagagat cgaacaaaaa atctacgaag aagccggcga
    15661 agaattcaat ttgaattcac caaagcaatt aggagtgatc ttatttgaaa aaatgggatt
    15721 acccgttatc aaaaaaacaa aaactggata ttccacagcc gtagatgtac tggaacagct
    15781 tcgtgaacaa gcaccaatcg tagaggatat cctcacctat cgtcaaattg caaaaattca
    15841 atctacctat gtcgagggac tgctgaaagt gatccaatca gatggaaaag tgcatacccg
    15901 ttatgtccag acattgaccc aaacaggacg attaagctct gttgatccta atttgcagaa
    15961 catcccgatt cgattagatg aagggagaaa gatacgtcag gcatttgttc ctagagaaaa
    16021 agactggctg atttattctt ctgactattc acaaatcgag ttacgcgtac ttgcgcatat
    16081 ctcaaatgat gaacatttga aagctgcctt cctagaaggt caagatatcc atgccagcac
    16141 agcgatgcgt gtatttggaa tcgaaaaggc agaggatgtc acaccaaata tgcgtaggca
    16201 ggcaaaagca gtgaattttg gtatcgtgta tggcatctct gattacggac tttcacaaaa
    16261 tctaggaatc agccgaaaag cagcacagca atacatcgat acgtactttg aaaaataccc
    16321 aggtgtcaaa gaatatatgg aaagaatcgt acgcgaagca aaagaccaag gctacgtcga
    16381 aacgctgtat caccgccgtc gatatctgcc agacatcaat tcaagaaatt acaatatccg
    16441 ttcttttgcg gaacgaacag ctatcaatac accaatccaa ggaagtgctg ctgatatttt
    16501 gaaaatcgcg atgatcgaat tggataaacg attaaaagaa acaggtcttc aagctacgat
    16561 gctgttacaa gttcatgatg aacttgtctt cgaggtaccg gaaaaagaac ttgaatcatt
    16621 agataaattg gtgaaggaag ttatggaaca agctgtttct cttcatgtac cactgatcac
    16681 tgacagcagt tggggaaaaa cgtggtacga agcaaaataa acattgttta agacagagca
    16741 atataatgaa ggtgaaaaga aaaaatgcca gaattaccag aagtagaaac cgttcgtaaa
    16801 ggattggaac ggttagtagt aggaaaaacg attcaaaaag ttcaggtctt gtggcccaag
    16861 atcatcgagc aaccagagac acccatcttt gaggcttctc ttgttggcga aaccatccaa
    16921 tccattggac gtagagggaa gtttttaatt tttcatctcg atcattgtga actgatctca
    16981 cacttgcgaa tggaaggaaa atatcaattt acgaaagaaa atacaccaat cgataaacat
    17041 acgcatgtac ttttcttttt tgaggatgga agccaacttc gctacaacga tgtacgaaaa
    17101 tttggtcgta tgacaatcgt cgaaaaagga gcaagtgcaa cttaccgagg aatcatgaaa
    17161 ttgggacctg agcctttgcc agatagcttc ttattagcag atttcgcaaa tggactgaaa
    17221 aagtcccaca aagcaatcaa gccattgctg ctagaccaac gtctagtgac tggtctaggt
    17281 aatatttatg tagatgaagc attatgggaa gcgaagatcc atccagaaca accggcgaac
    17341 acactgcgcc ccaaagaggt agaacaatta cgtctatcga tcattgatgt tttggatcgt
    17401 gcaatagaag ccggaggtac cacgattcgc agttatttga atgcattagg agaatcaggc
    17461 ggatttcaag tagcactgca tgtctatcag caaactggca aaccatgtat acgttgtgga
    17521 acgccgatcg tgaaaacaaa agtggcacaa agggggacgc actattgtcc gaattgtcaa
    17581 aggctaaaaa tcgcaaagta ggatttgttt taggtttgac gggtggaata gctaccggta
    17641 aaagtacggc agccaaagtc tttcagtctc atggatttcc tttgatcgat ggcgatgtga
    17701 ttgccagaga aactgttgag cctggaacgc ttgctctgag gaaaattgta tcagtgtttg
    17761 gccaagaggt attgcagcct gatgggcaac tagatcgagg gaaacttggg atgattgttt
    17821 ttccatcaaa ggaaaaacgg caaaaacttg atcagctatt agatccgttt atccgaaaag
    17881 cgataaaaga tcaaatcgcc tctctaagtt ccaatcatcc tttagtaatt gtagatatcc
    17941 cattgctcta cgaaggtcat tatgaccact atatggatgc agtagctgtc gtttatacta
    18001 cgccagaaac tcagcttcag cgtttgatga aacgtaatca actgacgcta gaacaagcgc
    18061 agcagagaat tagtagtcaa cttccaatcg aagaaaaaaa gcagcgtgcg gatattttgt
    18121 tcgacaacaa tggaacaaaa gaaaaacttg tgggacaaat cgaaaattgg ctttttgcta
    18181 atcgctttct ttgagtatct gtgtcaattt tgctatcctg agtatataga aagtgaggga
    18241 tagcgatggg atcattaaaa aatcaaaagg ataaagtcgt aggtaacgca aaagaaaaaa
    18301 tcggcgagat gctcaataaa gaaaatctaa tcgatgaagg tcgtacgcaa tcagaaacag
    18361 cagaagaaaa agaaaagcag taccaagcaa gaatcgatga aatagaagac cagcaggcag
    18421 aagaagaagc gaagcggcaa gatttgactc aagaacgaaa aaatccgaca cctgatcgta
    18481 gtaaaacacc gctcccagaa agtgaccaca ttcccgatga gcaaaaaatc gaggaacagc
    18541 gaatgaaaca ctatacagga atcgaagaga agttataaaa tagtaaaaaa tcgagatgtg
    18601 acttgaagca gatgagaagg tgttcatctg cttcaagttt tttgtgctag atgactaaat
    18661 cagcttttgt gtaagttatt tctcagtaat caaggtgatc agtattggaa aattgaaatt
    18721 tagtaaggac aggggacaca attacgcatg aaagtagaaa aaattgttct tatatccgtg
    18781 ttttttgggt aggacagtcg cgtttgtgat ttgtttcgtg ttataataca actagttgta
    18841 aagaaagaca catcctttat ataaaatgcg atgcaagagg tgagacaaaa tgagatgtcc
    18901 cagatgtcaa cataataatt cacgagtaat cgacagccgt caagcagatg atggacgtgc
    18961 gatccgtcgc cgtcgcgaat gtgagaattg ttcgttccga ttcactacat ttgaacgaat
    19021 tgaagctgct ccgttattag taatcaagaa aaacggggaa cgcgaagagt tcaatcgcga
    19081 taaaatttta agaggactca tccgttcagc agaaaaaaga cctgtagcta tggaacaaat
    19141 ggaacaaatc gtcaaccatg tcgaaaaccg cgtacgcagt ctcggagaaa acgaagtatc
    19201 gacgaatcta attggtgaat atgtgatgga agatttagtt gatttagacg aaatcgctta
    19261 tattcgtttt gcaagtgttt atcgccagtt caaagatatg tctgttttct taaaagagtt
    19321 gcaagatatc gtggataaag caaaagaaaa taataaagag aataataaag aaaacaatta
    19381 agaaacaaaa aggaggcatt tccttggaaa atgcttggaa agagcttcaa ccaaaaaata
    19441 tttaccaagg aagaaaagcg gctccgctga gagaagacag caagaacgcc ttgctgtatc
    19501 tttatcagcc actaatcggc ggagaagcct tgagtatcta tttcaccctg ttatcagaaa
    19561 tctctcaaga atctggcaaa ggtcctgaag ggatgcatgc agatctgctt agtagtctag
    19621 gctgcggtat cccgcaattt tatgatgcaa gaaaaaagct agaaggaatc ggcttactgg
    19681 atgtttattt taaagaagat agtgatctgg gacctcgttt tatttatgag ctgttagaac
    19741 cactcattcc taaagcattt ttcaaagact ctgttctggc ttttcttctt ctggaaaaag
    19801 ttggtgaacg acgattccag aagcttaccc aattctttga acctaaaagg catacagcag
    19861 agggatatca aaaaatcaca aaaaaatttt tagatgttta tcgcttcaat gaagcagtgt
    19921 acgctgctaa tcaagaaaac atcgaatcgc tgaataaatc ttttgaagaa tctgaatcag
    19981 tagcattact tgaagcagat tcgacattcg attggcagtt tttgaatgat tggctggcaa
    20041 aaaaacatat tgcaagtctt gatgatccaa tcatcaaaca agtcgacatg tatcatcagc
    20101 tttacggttt cgatgaattg acacttggcg aactgatcag tcaatcttat gactttagtg
    20161 aacaaaaagt atctgaaaaa gaactgcaaa aagccatttt ggcaatgcag agacgtccgg
    20221 tgaagcaaac aaaagaaaca gttgcggttt ctgaaaacag taatgaaccc tcgtcaaacg
    20281 aacatttgac aaacgcagcg cttcagctga taaaagaagc acagagcatt ccaccgatga
    20341 aatatctgga agcaatcaag tcagaaaaag gcggatatgt ttctaaacaa gaaaactggt
    20401 tactgcaaga tcttgtttct caatcaggtc tttccagtag cgtgatcaat gttttgctga
    20461 attatgtact agtgatcaag aataatgcta gcttaaatgc cagttatgta aatacagtgg
    20521 caaatgaatg ggcacaaaag aaaatcgcca cagcagaaga agcgatccag cacattcggc
    20581 aaataagtaa tcaagcaaaa caatcaaaac aaaagaagca aacaaattat caaactggca
    20641 agcgaaatgt tcgacgggaa aagcttcctg attgggtcaa ccagccaaaa gatgaaaaac
    20701 agatcagcag cgagaaaaaa gcagagattg accgccgttt taaagaatac ctggcaaaaa
    20761 aggagggtga tatctagtgg aagacgtagg caaagaattg aagaggatca tcagcaaacg
    20821 caattatcaa gaaaagtatg ctgaaatgat cgctgaagtc ttaaatgatg cggatgttca
    20881 agcttttcta agcgcacacc aagaagaatt gacacaagcg gatattgaaa aaagttacgc
    20941 taaattgtat gaattcgtgc aagaaaagcg taaatatcaa aaaaacgatc caacgatgat
    21001 cgctcccggc tatgagccga agctgacgtt gaattttcat tatgtggatg tcacttatgt
    21061 accaacggaa gcattgttag cacggcaaag acaagaagaa atcagaagcc gagtaaaagc
    21121 aatggatatg ccaaaagata ttcaatcggc ttcttttgaa aactttgaac gtacagatgg
    21181 acgaggatat gcgagcttag aagccctaga ttttgtagag aaatatactg ctgatccaaa
    21241 agggtatcat aaaggactgt atctagtagg aagttttggt attggaaaaa catatttatt
    21301 aggagcaaca gcgagagaat tagcgattgc tggattcacc acgacgctag tccattttcc
    21361 gacatttgcc gttgagatga aacaggcaat cggaaaagat caagtggcag aaaagttaga
    21421 tgcagtgaaa cgttcaccta ttttgatgat cgatgacatt ggtgcagatg cgatgagtag
    21481 ttggatacga gacgaagtat tcggtgttat ccttcaatat cggatgcagg aacagttacc
    21541 aaccttcttc tcatcgaatt ttacaatgga tgaattggaa cagcatttgt ccgtcactca
    21601 acgtggagat gaagagccgc taaaagcgaa acggataatg gaacgcatac gttatttgac
    21661 aaaagaaatc gaaatgactg gtcgcaaccg acgcaatggt tgaacagttg acaagtccaa
    21721 ctgacatttg acctagtagt aaaagcaggt cagcgaatca ttatatggac agaaatgacc
    21781 ggagacaatc accgcaacaa gtaatgcaga aacttaaacg gaggattttt tatgagggct
    21841 attttttcta gtatatctgt agttggtcta ttttttcttt tagcaggctg cggtagcgca
    21901 gatagcaaca caggttctgt tgattcgatt gatgcgggtc aaacagtaaa aacaagcagt
    21961 acgaaaacgg agagacaaag catatttgtc ggaacgattg aaagtatcac tcaagtagaa
    22021 gcagaagaag aaacgcttca gttgaaactc gtcgatgtca tggccaaaga agatccagcg
    22081 aatatgggaa cgtcattcag caatgacgga gtgatcttaa atgctactgc tgaaaaaata
    22141 agcgtggaca taaatgaatt gaaaatcggt gacaaagtcc aatttacatt agttgagatg
    22201 ccgatcatga cgatgtctat cccaccgcaa gttcctggag attcgattat tgatgtttca
    22261 ttgaacaaat aaccaataac attttttcaa aaataagaaa atgttgtttt atagaacgaa
    22321 aaggtatatt ttttaaggag atatgggaat ttttttattc ctagtctcct tattttattt
    22381 ggaaaatgct ataattatct agaaggagtg aagctatgaa ccatttagat ttatctcagc
    22441 gcttagccgc agtgggtgac tttgtccctg aatccgctcg tttagcagac attggatcag
    22501 atcacgctta cttacccgtt gcattgatat taagaggaaa aatcgaatat gcagttgctg
    22561 gtgaagtcgt tcaaggtcct tatgaatcag cacgtaagca agtcaaaaaa gatggattag
    22621 aagaccgtat caccgtacgc cttgcaaatg gtctagatgc tatccagcca caagatggta
    22681 ttacagctgt cacgattgcg ggtatgggag gttcactgat ccgagatatc ctagaagctg
    22741 gcaagttgaa tcaacgctta agcggacaag aacgtttgat ccttcagcca aacatcggag
    22801 agcgtacatt acgatcttgg ctgcaagaaa atcagtataa aatccaatca gaaaaaatac
    22861 ttgaagaaaa taaaaaaata tatgaaatca ttgtagccga aaaagaacaa aaaccagtta
    22921 cttacaatga aatggagctg atgttcgggc catttctgct tgaagagaaa agtgagatct
    22981 tcaaatcaaa atggacaaga gagcttcaac aaagaaaagc tatccgtgaa cagctcaaac
    23041 aagcagctaa tcaagaggca cgtcaaaaac aaatcgagca ggaaatccaa atgattgagg
    23101 aggttttaaa gccatgacaa tcagcggacg cactttcatc caaaagtttg aaagctattg
    23161 tcctcagtgg ctagctgaag aaggagaccc agtcggactg catatcggaa cattggataa
    23221 gccaatccaa cgagtaatga tgacattaga cgttcgtcca gaagtggtcg aagaagcaat
    23281 caagaaaaaa atcgatttgc tgattgctaa acatcctcca atctttcgtc ctgtaaaacg
    23341 gctggttact gatcagccac aagaaaaaat gtatgcagat ttgttgaaac atgatattgc
    23401 cgtctatgca gcacacacga acatggatat tatttgggat ggactgaatg actggttttg
    23461 tgaacttctc ggtattgaag tggagagtta tttagtaaag actcatgaga ttcattataa
    23521 aaaactggcc gtttatgtac ctgtggatca tgcacagaaa atgcgtgaag tattagctgc
    23581 agctggtgct ggtacacaag gtgattatac cggtacaagt tttacctcga ttggacacgg
    23641 tcgatttact ccggaagctg gtgcacaacc agcgattggg aaagtaggga agacagaaca
    23701 agttcaagag gcaaaagttg aggttatttt gccggaaact atcgaaaaac aagtgattca
    23761 agccatgcgg tcagctcatc cttatgagga acctgcttat gatcttttcg ctatagatga
    23821 accagtcgaa atgtttgggt taggacgagt aggtgaattg ccgcaagaaa tttctataga
    23881 ggcatttgta gaacaagtaa aagaagcgtt ccaactagat ggactgagaa tagtccagcc
    23941 aaaaaatgca aaatcctctg tgaagcgcat tgcgatttgc ggtggaagcg gtgaaaaatt
    24001 ttatccgcaa gcaatcgccc aaagagcaga tgtctatatc acgggagata tctattatca
    24061 tacggctcat gatatgcaaa gcgctgggtt gattgcaatt gatcccggtc attatattga
    24121 atcgttatgc aagcaacgat ttatcgagaa atttgaatct tggaaacaag aagaaaattg
    24181 ggatattaac tttttcgttt ctgaaacaaa tacaaaccca tttcaattta attaaaagga
    24241 gagaatacta tgtacgaaaa tcttttgcca cgttttttac gatatgtcaa aacagaaaca
    24301 cgctccgatg caacaagcaa aacgactcca tcgactcaga cacaagttgc ttttgcgcaa
    24361 gaattgaaaa aagaattaga agagttaggc ttagctgatg ttttctataa tgaagataac
    24421 ggattcgtta tgggaacatt accaagcaat gttgatcatg aagtgcgttc cattggattt
    24481 atttcccaca tggatacagc agattttaat gcagtaggag tcaatccaca aatcattgaa
    24541 aactatgatg gcgagtctac gatcgtacta gatgcggaag aacgctatac attgaataca
    24601 aaagattttc caaatttgaa aaattatgaa ggccacacat tgatcacaac agatggcagt
    24661 acattactgg gagcagatga caaatcaggg attgcggaaa tcatgactgc aatggagatc
    24721 ttgatcaata atccttctat ccctcacggt accatcaaag tggcttttgg tcctgatgaa
    24781 gaaatcggtg tcggagcgga taaattcgat gttgagcaat tcgatgtaga ttttgcttat
    24841 acagtcgatg gtggtccttt aggtgagttg cagtatgaaa cgtttagtgc tgctcaagca
    24901 aatattacga tccaagggaa aaatgtacat ccagggacgg caaaagatac aatgatcaat
    24961 gctctacagt tagcaatcga ttttcataat cagcttccag ccgatgaagt tccagaaaaa
    25021 acggaaggat acgaaggttt cttccatttg atggcattgg atgggaatcc tgaagaagct
    25081 tctatgagtt atatcattcg cgatcatgat cgtgaaaaat tcgaagcacg caaagcacaa
    25141 atcacacata tccaagaaga gttgaatcag cgatttgatc aagaacgaat caaagtagat
    25201 atgtatgatc aatattacaa catgaaagaa atcattgaaa aagatatgag catcgtggaa
    25261 ttagcgaaac aagcaatgat cgagcttggt atctctccag tgatcgaacc agttcgtgga
    25321 ggaacagatg gttcaaaaat ctcttacatg ggtattccga cgccaaatct atttgccgga
    25381 ggagaaaata tgcacggacg attcgaattt gtttctttac aagtaatgga aaaagcaaca
    25441 gacgtgattg taaaaattgc agaattgaac acaaagagtt aaaatcgaaa aagaacagct
    25501 tatgacaagg cgttcgtccc aatgatggaa tgtcatctgt ctaaggctaa agggcaggac
    25561 aatggaattg gttgtcccgc cctctttttt gaagagtaaa taggaggtaa agaatggaaa
    25621 agctgctagt tttttataac aaaagttcag ggaaagatga gggtgagaaa ttagcacgct
    25681 ggtttcaaga ttatgtaaaa gaaaagcagc cagaattata tgtagaacta gctgaaactg
    25741 gcccatcgat tgatgatgcg gaattagtca agaaagcaga agccatagaa gctgatactt
    25801 tagtaattat aggtggagac ggcacgattc atcatatcgt ccaagcgttc caaaaaacat
    25861 tatctcaata ccatgtcgga ttacttccag gtggaactgt caacaacctt gcacgtgttc
    25921 ttgagattcc cctcaaaaaa gaagaagctg ctgacgtgat cctggaaggc aatgtacgga
    25981 aaatagacta tggtcaagtc aatgatgaag tcattatctc tacactgaca atcggaatct
    26041 tagcagatac agcagtttgg atcagtcaag aagaaaaaca gcgttacggc tcgtggatct
    26101 ttatcaagaa gttttttaag ttattgctga agaagaaaaa atatcatttg gatttagaaa
    26161 cagacgagga gcattggaaa ggaaaatccc aactgttgac agtcacgatg tctaactccg
    26221 tgggcggttt tacaaatttt gacgactcag cgaccctaga tgatggaaaa tttcatgtaa
    26281 cgatcgtacc ttccttagat attttccgat ttatttttta tttgcctaga atcatgagag
    26341 gcaaattcta tacgatcccc ggcattactt atttcacagc caaacgcatg aaaatccaag
    26401 cgcaggaaaa atctgtgggc actcgaacgg atggagacac gacagatgat cttccagttg
    26461 aattatctgt tgtatcaaaa ggattaacag tttattcacc aaagaaagaa gaaaaagctt
    26521 agaaatagta gttaacagaa agttggaaga agagtaatgg atgtattgtt acacaatcct
    26581 ttgtggtttt accttttcat tgtgaatttt tacttatttt gtctgatggg gtatgataaa
    26641 catcaggcta gaactagcgg ctggcgtgtt cctgagaata atttgatatt tgtcagcctg
    26701 ttaggcggtg gtttgggagg attgatctct cgaaaagttt tccatcataa aactcggaaa
    26761 aagaagttta ctataggatt cgctgcaggt gtacttgttg atttgattct catttatttt
    26821 ttccattaag gagcaggaga tgtcgtctaa aggaacgaaa actaaaatca ttctgaatct
    26881 gcttttgctg attgtgattt ttgccattat ttattattta atcaatcaat catttgcaga
    26941 tatctttgcc gaattgctgt ctaccagtgt tgttgttttg gcagcggctg ttatattagg
    27001 gacagtttac caaatcgctg aagggcgctc aatcaaagaa attgctgaac ctttcgcgcc
    27061 gaattttaca acgaaagatg ggttcttgac ctctagctat attgcgtttt accgaatcgt
    27121 ttcgtttggt acaggaacgc tgatctcgga gatttatttt tataacaaaa aaggaatgaa
    27181 gctatcccag ggagcagggg tgacagcact ccatatgatt atgtacaaac tggcagtgat
    27241 cacttatgca ttagctggct tggtcatcca attctctctt ttttattcta atggtccatc
    27301 aatgatctgg tttatcatcg ctggtatcct tttgacgatc gtgattgttg cctttttatt
    27361 ggcgatgtcc gtcagcttga atgttcaaat attttttgtc agcttatcga ataagttatt
    27421 taaaagtgag cgtttgcgga aatgggtgga tacatgcaat cagcagatct actctttaag
    27481 ggaaacggtc caaaccatta tccaagacag gactgcgcta ttgcggattt atctatggaa
    27541 tatgttaaaa cttgtatttt ggtatgtact tccttatttg attctgattg aaaaccatcc
    27601 taaaatagat tttctactga ccatttcgtt tatcagcttt gcagtagtgc tttcaggggt
    27661 tattcctacg cctgctggta ttggttcctt tgaatttgtc tatttgttat tattcagacc
    27721 agttgttgga acagttgacg cagtttcttc gcttttactg tatcgtttca gttcctttat
    27781 cttgccattt ctctatggat ttgtgtatgt gattgctgaa cggcgaagag tgatcaaaga
    27841 agaaataata gaagtaaaaa aagagaataa agaaaatcaa gggtaggtct tttataagat
    27901 ctaccctttt tatcaaatca aagaaaaacg ttatggataa gataatttta cgtttgtttg
//
LOCUS       DACDIG010000032        27546 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.032, whole genome shotgun sequence.
ACCESSION   DACDIG010000032 DACDIG010000000
VERSION     DACDIG010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.032"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            131..505
                     /locus_tag="IV247_001469"
     CDS             131..505
                     /locus_tag="IV247_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP8777546.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="IV247_001470"
     CDS             complement(619..1248)
                     /locus_tag="IV247_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP8777547.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="IV247_001471"
     tRNA            complement(1382..1454)
                     /locus_tag="IV247_001471"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="IV247_001472"
     tRNA            1668..1741
                     /locus_tag="IV247_001472"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            1696..1845
                     /locus_tag="IV247_001473"
     CDS             1696..1845
                     /locus_tag="IV247_001473"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777548.1"
                     /translation="MTVNHDVVGSSPTAGVNKAYAKKKKSNHLGFFFFYYYDESSFLF
                     LSRML"
     gene            2338..3666
                     /locus_tag="IV247_001474"
     CDS             2338..3666
                     /locus_tag="IV247_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP8777549.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="IV247_001475"
     CDS             3868..4701
                     /locus_tag="IV247_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAP8777550.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..5454
                     /locus_tag="IV247_001476"
     CDS             4717..5454
                     /locus_tag="IV247_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAP8777551.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5609..6577)
                     /locus_tag="IV247_001477"
     CDS             complement(5609..6577)
                     /locus_tag="IV247_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAP8777552.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6809..7642
                     /locus_tag="IV247_001478"
     CDS             6809..7642
                     /locus_tag="IV247_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAP8777553.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7676..8515
                     /locus_tag="IV247_001479"
     CDS             7676..8515
                     /locus_tag="IV247_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8777554.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8541..9101
                     /locus_tag="IV247_001480"
     CDS             8541..9101
                     /locus_tag="IV247_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP8777555.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9300..11021
                     /locus_tag="IV247_001481"
     CDS             9300..11021
                     /locus_tag="IV247_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAP8777556.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11186..12328
                     /locus_tag="IV247_001482"
     CDS             11186..12328
                     /locus_tag="IV247_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP8777557.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12344..13555
                     /gene="thiI"
                     /locus_tag="IV247_001483"
     CDS             12344..13555
                     /gene="thiI"
                     /locus_tag="IV247_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAP8777558.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            14128..14775
                     /locus_tag="IV247_001484"
     CDS             14128..14775
                     /locus_tag="IV247_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP8777559.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            15168..17813
                     /locus_tag="IV247_001485"
     CDS             15168..17813
                     /locus_tag="IV247_001485"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAP8777560.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FYRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            18123..19436
                     /locus_tag="IV247_001486"
     CDS             18123..19436
                     /locus_tag="IV247_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAP8777561.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            19426..20079
                     /locus_tag="IV247_001487"
     CDS             19426..20079
                     /locus_tag="IV247_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8777562.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            20134..20826
                     /locus_tag="IV247_001488"
     CDS             20134..20826
                     /locus_tag="IV247_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAP8777563.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            20976..21176
                     /locus_tag="IV247_001489"
     CDS             20976..21176
                     /locus_tag="IV247_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8777564.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(21430..23109)
                     /locus_tag="IV247_001490"
     CDS             complement(21430..23109)
                     /locus_tag="IV247_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP8777565.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(23111..23323)
                     /locus_tag="IV247_001491"
     CDS             complement(23111..23323)
                     /locus_tag="IV247_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAP8777566.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            23716..25446
                     /gene="efrA"
                     /locus_tag="IV247_001492"
     CDS             23716..25446
                     /gene="efrA"
                     /locus_tag="IV247_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAP8777567.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            25443..27203
                     /gene="efrB"
                     /locus_tag="IV247_001493"
     CDS             25443..27203
                     /gene="efrB"
                     /locus_tag="IV247_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAP8777568.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            27298..27495
                     /locus_tag="IV247_001494"
     CDS             27298..27495
                     /locus_tag="IV247_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777569.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
BASE COUNT         9079 a         4669 c         5758 g         8040 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     5461 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     5521 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     5581 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     5641 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     5701 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
     5761 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     5821 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     5881 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     5941 catatgcttt aacgatcggg atagtcccat ctacagcgga aatgtccagt cgctttgttt
     6001 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     6061 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     6121 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     6181 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     6241 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     6301 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     6361 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     6421 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     6481 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     6541 tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt acagacttcc ttacgaataa
     6601 ttttgcatag ccgttaaatc ttattgtata ctatataaaa gagttttgct atcagtgaag
     6661 aataacgaat agcctaactt atcataaaaa agctgacctt attaaaatgg aaaagactca
     6721 accttgacct cctaatacaa tggagtatct ggttgagttt cttttacaag aaagcagcca
     6781 aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc ggattaacat cgtttaaaga
     6841 acatgacaaa ttaactggta aaaagaataa cactctttat gaatatgcag ggtttctacc
     6901 cttagtggaa ttagatactg cctattatgg aattccccga aagtcctcag ttgagaattg
     6961 gataagtgaa gtacctgaat catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg
     7021 tcaaggagac tggaagcctt attatcagac cgaagaagag atgattgcgc attttttaga
     7081 tgttatgtcg ccaatggtag atgctggaaa gatgttttgc tttttagtcc aattcccctc
     7141 atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc aaacttagaa agtggtttga
     7201 gggttatcct gtttctatag aattgcggga ttattcttgg tacagcgatg aatttcttga
     7261 acgtacaaga aactttatgt ctagagccgc tttttcttta gccatcatcg atgagccaca
     7321 gttgctgaat acaacgattc cttttgatcc atttgtcacc aatccgaact ttgctctttt
     7381 tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa
     7441 aagaacgtta tatcgttatt cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt
     7501 agttgattct aagacaaaag aggtaggtgt gatttttaat aataattctg gtggtgatgc
     7561 ggctgaaaat gcattagcac tgcaaaaaat attaaatatc aagccagatg gactcaatcc
     7621 aacccaaatg gatctgtttt aagggcttat tttattcaag aaaggggcga agtctatgat
     7681 caaagctatt ttttttgata ttgacggaac attggtcacc aatcgttcaa aagcgttgga
     7741 atcaacaaaa caagcaattg aatatgctcg gaaaaacggc attttatgcg gtgtagcaac
     7801 ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa ttggaattag atatgtatgt
     7861 cgtgtataat ggtcaactgg tttttactgc agatcgcacg attatcgacc acccttttga
     7921 acaaaaagta ttggaacata ttgttgaatt tgcagatgag aaccatcgac aaatcgtttt
     7981 tggtgcgaga aatcggttag atggatcaac gacgatgcta ttaggacaat cgatttttat
     8041 caagcgacta gtcagctttt taccgcgtaa attcccggta cgtctgatga aaaaaatcct
     8101 ccaggttttt agccctcacc gacaaaagga tcgttatgaa acgttagaga tattaaaaga
     8161 accaatttat caatgtatat tgctcagtcc ggagtcagaa cagaaaaagt tagaacatcg
     8221 ctttcccgag tgtacgtttc agcgttcaaa tacgtatact gtagatatca tcccaaaagg
     8281 tggttcaaaa ttactaggca tccaagcttt cgcaaatgct gtcgaaattg aaatggaaga
     8341 aattatggca ttcggcgatc attacaatga tatcgagatg ctgaaaggtg taggaattgg
     8401 agtggccatg gggaatgctc agatcgaagt aaaacatgct gcagactttg tgactcaatc
     8461 aaatgaagaa gatgggatat acgaaggact gaaacacttc ggtctgattt attagtcaat
     8521 gaaagaagga aaaaatcagt atggaagcag agacaccttt tcaaaaagta gaaacattcc
     8581 acaaaacttt tgatccaaga agaccagaga tacccacggc ttttacagaa aatgaagctc
     8641 atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt tgttgcagct acttcaccta
     8701 ataaagaaca actgcatgaa gctgttatgc tgctgcacga agcattagat aaagcagaag
     8761 aaaaaatact tggtaaaaca cagtggggaa gtgatccgct ggttgaacaa gtagatgcac
     8821 tgacagatat cctttacttt acttatggtt ctttttcatt actaggaata gtgcctgatc
     8881 gaatattcga aatcgtccat caggcaaata tggggaaatt atttccagat ggaaaacctc
     8941 attatgaccc tgtaacaaat aaagtgttga aacctgaaaa ctggcagatg gaatatgctc
     9001 cagaacctaa aatcgcggca gaaatccaac gtcagattca agaagcgatt gctaagaaga
     9061 aatccgctga tataggcatg tgcacaaatg aaaaagagtg atcagagttt cactattttt
     9121 gttatcttta tacgatggaa aaacaaaaga ttttcgttta agttagtgaa tgcatgaaaa
     9181 tagtatgcta taatctagaa tagatgtctt ttgcacgaga gacgtattat ttggatttat
     9241 gccaagcaga acgccatctg ttgcataaaa gatgcatact gaaaacggag gaaccataaa
     9301 tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat tatagtcgcc attgtcgtct
     9361 ttttggttag ttacttgatg aagaaaaaaa accaagatcg attggatgcc ttagaaaaac
     9421 gcaaggaaga attatttgac ttgccagtga ttgaagaagt cgatgatgtt aaaaaaatgc
     9481 atctggttgg gcaaagccaa aatacctttc gtgaatggag tcaaaaatgg aatgacatat
     9541 ctacttcctc gttcgctgaa ctagaaagtc aaattttcga agtggaaaac ctcaatgaaa
     9601 gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc agaagctaca atggaccaaa
     9661 tggagaaaca agtgacggaa atccgtgaag gtctaaagga attgcgggaa acggaagaac
     9721 gcaactcact agctgttcag caggcacttg acgtctatga agaactgaaa gaaaaagtca
     9781 gagaagacga gcatgaatac ggaccagctc tttttgaatt gcaaaaacag atcaaagtga
     9841 tcgaaaaaga atttacgcag tttgtgactt taaacacttc tggagaccct gttgaagccc
     9901 gcgaggtttt ggataaagca gaaaaacata cgtatgaagt ggaagctctg atgaaaaaaa
     9961 ttccaccgct gtatgaggag ctccatacca ctttccctga acaacttgaa gaaatcagtg
    10021 atacttacga gaaattgatg gaagaacaat atgtattccc agaagaagat cttgctgaag
    10081 atatcgcaaa agtaagtcga agaattgaaa attcattagc caatcttgaa aaaacagaag
    10141 ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt gattgattcg ttatatgata
    10201 tcttagaacg ggaaatggaa gctcagagat atgtaaaaac taaccaatca acgattgcag
    10261 agtatatcaa acatacaaca aagaataacc gtcaattatt gattgagctg gatcacacat
    10321 ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt acgaggtttc caaaccgaag
    10381 tggaagaaat ggaacgtcaa aatgaacaga tgattccaca gatccgtcaa cacgaaattc
    10441 cttattcaga agtgcgcacg ttctataaaa cagtcttcaa agtcttagaa gatatcgaaa
    10501 agcaacaagt agagatcgac gactcattac atgaattgag aaaaggcgaa aaagaagcgc
    10561 aagaaaagat cgatacattc gaattcaaat tgcgcagttt gaaacgtttt gtagaaaaac
    10621 aacgtttgcc tggcttgccg aatgattacc tagaattctt ttttgttgct actgaccgta
    10681 ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa catggaagag gtcaatcgtt
    10741 tagttgctct atgtgaagaa gatctagaat tattagataa gaagacacat gatttagtag
    10801 atgcagcagc attaacggag caaatgctcc aatatgcaaa tcgttatcgt catacacacg
    10861 aagaagtgag agaagcaatt gataaaagct actatttatt caataaagaa tatcactatc
    10921 aagaagcatt agacgaaatc ggtacagcat tagagcgagt agaacctggt gctttcaaac
    10981 gcatcgaaga tttttatttt aatcatccag acttagtata aagcaaaaaa gttagtaaac
    11041 cagcaagagc aagacggatt attgtcttgt tcttgtcttt ttttgtatat ttgaaaattg
    11101 aaacaagtga aaatttcacc tgctcttgaa agcaccttgg acaaccgtta taatagaaaa
    11161 gaatttcaaa aaagaggggt ttcttatgat atattttgat aacagtgcaa caaccccaat
    11221 ctttccgcaa agcttagatg catatgtaaa aaccagtcag cggattattg ggaatccatc
    11281 aagcttgcat gatttaggca atcaagctaa ccggttgttg cagcaagcaa gaaagcagat
    11341 cgctgatttg atccatgtag aaccagaaga aatctatttt acttcaggcg gaacagaagg
    11401 agataactgg gtattaaaag gaacgatgat cgaaaaaaga gaatatggga atcatatgat
    11461 catttcagga gtcgaacatc cggccgtcag cgagacagca gaacaattaa aagaattagg
    11521 aatcgaagta agtatcgccc cagtggataa acgcggattt gtacgtgtcg acgaattgaa
    11581 agaattaata agaaaagaga cagtattagt ctctgttatg gctgtgaata atgaagtagg
    11641 atcgattcag cccatccagg aaatcagtga tcttttggat gattttccta aaattcattt
    11701 ccatgtagat gctgttcagg cgattggaaa agtaccattc tcgcaatggt tgacagaaag
    11761 agttgatttt gcaacatttt cagctcataa attccatggc ccacggggaa ctggtttcat
    11821 ttattggaaa aagggtcgtc gtttagcacc attactgaca ggtggagggc aagaaagaaa
    11881 tcagcgaagt gggacagaaa atgttccagg tattgtggct atggttaaag ctttacgttt
    11941 atcattagag ataaaggaac aaaaacctga gcagacacaa cgcttaaaag tagctctaat
    12001 caatgcgttg aagacgtatg agaaagtgac gattttttca gaaggaactg actatgcacc
    12061 gcatatttta tgttttgctc taaaaggaat tcgcggcgaa gttctcgtcc atgccttaga
    12121 agaaaaacaa atatttgttt ctactactag tgcctgctcc tcacgtaaac atatggcaag
    12181 cagtacattg cacgcaatgc atgtaccaaa tgatctggct acatctgctg ttcgaatcag
    12241 cttagatgaa aacaattcgc ttgcagaagt cgagcaattt atgattattt tcaagcagct
    12301 ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt cttgtgaaat acagtgaaat
    12361 tatggtccgt tatggggaac tttcgacaaa aggaaagaat cgaaaaagct ttatcatgca
    12421 actagcacaa aatgtcagac aagctttgaa agactttcca gaaatcaaaa tccatgctga
    12481 tcgggaccgg atgcatcttt tattgaatgg cgcagacggg cagctagtta ttccaaaact
    12541 agcaaaaata tttgggatcc aaaatttctc tccaagtatt cgtgtagaaa aagatgtcga
    12601 agttttgaaa caagctgttc aagacatcat gaaagaaatt tatactggga aagaaacatt
    12661 taaaattatc gctaaaagaa gcgatcacca atttgaattg acaagtaatg aattgaatca
    12721 aacattaggt aatgctgttt ttgatatttt cccgcatatc caagttcaaa tgcgtcaacc
    12781 agatattcca ttacgtgtag agattcggcg tgatggcgct tatctatcgt atgaaacgat
    12841 caagggagct ggaggattgc ccgtaggaac aagcggaaaa ggaatgctta tgctttctgg
    12901 ggggattgat tctcctgtag cagggtactt tgccatgaag cggggagtgg aaattgaagc
    12961 tgttcatttt gctagtccgc cgtatacaag tgaacaagca ctgcaaaaag caaaagatct
    13021 aacagccaaa ctagcacctt atgtgggaac gatccatttt atcgaagtgc catttaccga
    13081 gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat tggatgacaa ttactcgtcg
    13141 aatgatgttg cgtttgaccg atgaaattcg ccgtattcgt catggtttag tcattttgaa
    13201 tggtgaatca ttaggtcaag tggcttcaca aactttgcaa agtatggtgg ctatcaatga
    13261 agtaacgaat acgccaatca ttcggcctgt agcgacaatg gataaacttg aaatcattga
    13321 agtagcagaa cagatcgata catttaatct ttctatccag ccttttgaag attgctgtac
    13381 gatttttgct cctccacagc caaaaacacg ccctcaactg gaaaaggtcc atcaatatga
    13441 agagcgttta gcaatagatg aaatgatcca acgagcacta gctggcttga agatggaaga
    13501 gatacaagca gaatcgtcta atgaaaaaac tgaagaattt tcagaattgc tataagaggt
    13561 tatgaaaata gtgttttgac cagagtatta gagaaacaaa acgaaaaaat agctccatgt
    13621 attttaccgt ttttgaacta atgtcgaaga ttagctgatt gaataccatt tatcaagaag
    13681 ttatggaaaa gccggttctt ttttactaac tgaaaaataa aattttattt agagatcaag
    13741 ctacagttga taaatgatat aaaaaagaga atttcccatg aaatagcgag gttgtgacag
    13801 aagtgggcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttttca aatttttgtg
    13861 aaatttcggc ttatttttcg aagaagttac ttctggaaca ccgtgtatca gaggttgcga
    13921 aaaagctgcc ctgcatcaag taataagaac agccaaacaa aaatagttcc tcatatttta
    13981 cgtttggttg agcttattac cgcagatgca agcttttgaa caccgtttta tttgtctcac
    14041 acccttttcc ttcctacttg taaccttgct cccgtgttag aaacatgtta cactaaatat
    14101 gtgaaattat taacaaggag cgtcgttatg cgagaaaaaa cagatacact gccaaaagca
    14161 actgcgaaaa gattaccttt atatttacgc tacttgaaga tgctggatga ctcaggtata
    14221 agtagaatca aatcaaatga gttcagcgaa atcacccaaa ttccttctgc aacgatccgc
    14281 agagacttct cgcaattagg cgagctaggc agaagcggat atggctatga tgtccctttt
    14341 cttatcgatg tattcaataa tatccttaat actaaagaag aaaaacgaat cgcattagta
    14401 ggatatggaa atcttggcaa agcattgaaa cacaacaatt ttagaagaaa tgaaaatcta
    14461 aatatcgtgt gcgtctttga taatgatcca gcactgatca atcgagtaat tgatggagaa
    14521 atgatctatc cgattgatcg tttcgcggag atagccaaag caaaaaatgt gacagtagca
    14581 atctctacgg taccaagtaa atattcacaa tctgctattg atgaaattgt caaaggaaat
    14641 gtaaccgcta tacttaattt tgcgcctgac agagtaactg tcccagctta tgtcaatgtt
    14701 caatacattg atttgacaac cgagttgcag acgcttattt actttgatga gaattactca
    14761 gaagtttttt cttaggaaaa tttgagtagg cttattgatc ttgcgagaac tcgtcgtaat
    14821 tctttcaaaa attttttagt actaaatgaa gtggtttttt cacgaccact tgttgactaa
    14881 aaaaaaaaac cattgtagaa tgaagcataa cgctgaacaa gaggagtaac tggcacattc
    14941 attcttagag agagaactat ctgctgaaag gttctcaatg acaaccagtg aaggtagctt
    15001 gggagtttct gtcttgaatg gagtagagat agacgagtcg tgatcgttaa ctcacttgag
    15061 aggcaataat gattgattat tgcaatttag gtggtaccgc gtaaatcata cgtcctagaa
    15121 tacgaaagta ttttaggacg ttttttagtt taaaggagga aaacagcatg acggaagaaa
    15181 ataacctatc aacaaaatat aatccgcaag aagttgaagc tggacgttat cagcattggt
    15241 tagaacaaga tttgtttaaa ccgagcggag ataaaaaagc aaaaccttat tcaatcgtaa
    15301 tcccgccccc aaatgttact ggaaaacttc atttagggca tgcttgggat acgaccttgc
    15361 aagatatgct gattcgtcaa aagagaatgc aaggatatga tactttatgg ctgcctggta
    15421 tggaccatgc aggaatcgcg acacaagcaa aagtagaagc gaagcttaga gagcaaggga
    15481 tttctcgtta tgatctaggt cgtgaaaagt tcatcgatca agtgtgggaa tggaaagaag
    15541 agtatgcgag ccacatccgc gaacagtggg ctaaaatggg cttatcttta gattacagta
    15601 gggaacgttt tacattagat gatggattat ctgaagcagt acgtaaagtt tttgttacct
    15661 tatacgaaaa aggtttgatt tatcgtggtg aatatatcat caactgggat cctcaagcac
    15721 gaacagctct atctgatatc gaagttattc ataaagatat cgaaggcgcc ttttatcata
    15781 tgagctatga actagcagat ggatcaggcg tagtagaaat tgcgacaacc cgtccagaaa
    15841 cgatgttggg agatacggcg attgctgttc atccagaaga cgagcgttat caagcgctca
    15901 tcggcaaaaa agtgattcta cctttagtca ataaacaaat cccaatcatt gcagatgaat
    15961 atgtagatat ggaattcggg acaggtgtag taaaaatcac tccagcacat gatccaaacg
    16021 actttgaggt aggtaatcgt catgatcttc cacgcgtaaa tgttatggat gatgatggta
    16081 cgatgaatga actagcagga aaatatgctg gaatggaacg ttttgctgct cgtaaagcaa
    16141 tcgtgcaaga tctgaaagaa atcggtcgat tgattaaaat cgatacgatg atccacagtg
    16201 tgggtcattc cgagcggaca ggtgtcgttg ttgaacctcg gctgtctact caatggtttg
    16261 taaaaatggc gccgttagcg aaaaaagcca ttgataacca agaaacagat caagcagtgg
    16321 aattcttccc tccaagattc aatcaaacgt ttttacgttg gatggaaaat gtccatgatt
    16381 gggtaatctc acgacaatta tggtggggac atcaaattcc tgcatggtat cacaaagaaa
    16441 caggtgaaat gtatgtaggg atggaagcac cagcggatag tgaaaactgg gtacaagatc
    16501 ctgatgtttt agacacttgg tttagttcag ctttgtggcc tttctcaaca atgggctggc
    16561 cagatacgga aagcgaagat tataagcgat acttccctac tagcacttta gtaaccggtt
    16621 atgacatcat tttcttctgg gtaagcagaa tgattttcca aagcttggaa tttactggcg
    16681 aacgtccatt tgagaatgtc ttgattcatg gattgattcg tgacgaacaa gggcggaaga
    16741 tgagtaaatc attaggtaat ggaatcgatc cgatggaagt aatcgaaaag tatggagcag
    16801 atgctctccg ctggttctta tcgaatggtt ctgcaccagg acaagatgtc cgcttcagct
    16861 atgaaaaaat ggatgcttct tggaacttta tcaacaaaat ctggaatgct tcacgtttcg
    16921 ttatcatgaa tatcgaagga atgaccgtag aagagatcga tttatctgga gaaaaatcag
    16981 tggctgaccg gtggatcctt actcgtttga atgaaacgat cgaacgagta actgagctat
    17041 tttatcgttt tgaatttggt gaagcaggtc gacaactgta taactttatc tgggatgatt
    17101 tttgtgactg gtacatcgaa atgagtaaag aagtacttta tggagatgat gctgtttcaa
    17161 aacaaaccac ccgtagcatc ttagtccata cacttgatca gatcctacgt ttattgcatc
    17221 caatcatgcc attcgttaca gaagaaatat gggaacatat tcctcatcag ggaaattctc
    17281 tagtagtggc agaatatcct gttgtacgtc cagagtttga tgatgaaaca gcttctaaag
    17341 ggatggaagt attgaaagaa ttgattcgag ccgttcgaaa tattcgttca gaagtgaata
    17401 caccattgtc taaaccaatt acgttgctaa ttaaaacaaa cgatgctaaa atcgatcaat
    17461 ttttagttga aaatacaagc tatatcgaac gcttctgtaa tcccgaagag ttgacgatat
    17521 caagtgagat cactgcgcca gatctagcga tgtcagctgt tctgacaggt gcagaaatct
    17581 tcttgccgtt agcaggattg atcaatattg aagaagaaat caaacgatta gaaaaagaac
    17641 tggaaaagtg gacaagcgaa gtaaaacgtg tccaaggaaa actttctaat gaacgttttg
    17701 tttctaacgc gccggaagaa gtagtagagg cagagcgtgc aaaagaaaaa gattatttag
    17761 agaagcaaac ggctgtcaaa gaacgaatcg aaagtttgcg tactattcaa taagaataga
    17821 aaaagagagt gtgaaatcag ctagctatta ctaaaagcta gctgattgaa catcgttaca
    17881 ggaaagagtt tgagcctcac tcttagcttc gattcataag gagatgtgac ataaattttg
    17941 tcgcatctct tttttagtct actcttatta tgtaggagaa ggagggaggt ggatcatgta
    18001 tcaagagtca ataaagtagt tgataaaatc gttcctctct ctgtttcttt tctctttgtc
    18061 atacttttat ttttcttatg tgttctattt gttataataa aagagaaatg ggggaattgt
    18121 tgatgacgat tgaagaagca atagaatgga tccatagtcg attaccattt ggttcacgtc
    18181 ctggccttga ccgagtcgaa gctttattag aagtggtagg gcatccagag agaaaagtaa
    18241 aaacgatcca tatcgctgga acaaacggaa aaggttctac tgtagcttat ttgagaagcc
    18301 tattagaaga ggctgggctg acagtaggta catttacttc accttatatc gaaacgttca
    18361 atgaacggat cgcaatcaat ggcaaaagca ttccagacaa tgatttgatc cagcttgtaa
    18421 aaaaatatca gccactgatc aaacaacttg atcaatttga agcagtagct ggtattactg
    18481 aattcgagac attaacagca atggcatttg attatttttt gatgaagaaa gtggacgtag
    18541 cgattatcga agttggctta ggcggcttat tggattctac caatgtagct gtaccaatgc
    18601 tgacgggaat aacaacgatt ggacttgatc ataccgaaat acttggagat acaattgaag
    18661 aaatcgctgc acaaaaagct ggaatcatca aacaaggcgt tccagtagta acaggaaata
    18721 ttgccccaga agcttttact gtcatccaac aaacagctaa agcaaaaaat gcaccgcttt
    18781 atgtatacgg ggaaaattat aaaataaact atctgcatcc tgatgagatg tggggagaag
    18841 tatttgaatt ttttaatgaa ataggaaaga tccctcaatt aaccactccg cttttagggc
    18901 aacatcaagc tgaaaatgca ggagtagcca tcgaattgta ttttgtttac tgtcaattgg
    18961 aaaagctgcc tttccgagaa aaagatgttt tgaagggatt gaagaaatct cgatggccag
    19021 gaagaatgga gcgtttgagt aacgagccat tagttgtttt agatggtgcc cataatacgc
    19081 atgcaatgaa ccggcttgtt gagaatttga aaaaagaatt caaaggatat caaatcaaca
    19141 ttttgttttc cgccttacga actaaaaatg ttagagaaat gctagaacga ttgcagcaag
    19201 taccaaatgt ccatatttat ttgacgacct ttgaacatcc aaaagccatc gaattaagtg
    19261 aagtatcaga tttagcaagt gagagagtat caatcgtttc attgtggcaa ttcggattag
    19321 cagatatatt ggaaaagatg acaaatgaag acgtattagt tgtgaccggt tcactttatt
    19381 tcatttcaga agttcgccat ttattaatac aattaggagg agatcatgaa gtataatgca
    19441 gtgatttttg acatggatgg tttgctgttc gacacagagt tagtgtatta cgaagccaca
    19501 caagtagtag cagacaagat gggttttcct tatgataaag acctgtattt aaaattctta
    19561 ggtgtttcag atgaagaagt atgggcgaat tatcatatga ttttctcgga atttggcaaa
    19621 acaagtgtac aaagattcat agatgattca taccaggaaa cgcttgaacg tttttcgcaa
    19681 ggagcggtac aaatgaagcc cggtgtgttg gaactcctct cttttttaga agaacatcag
    19741 attcccaaag ttgtcgcttc cagtaaccaa cgagcagtca ttgaactttt attgaagaag
    19801 aacaatctgt ttgaacgatt tgagacgatt gtttctgcag aaaatgttca acgagcaaaa
    19861 ccagacccag aaattttctt gttagctaat cagaagctag gtacagcgaa gaaagaaaca
    19921 ttgattttag aagattcaca aaatggtatt ttagcagctt ctgcggctga gatcccggta
    19981 gtcatggtac cagatctgct tccaccatcc caagaattga tcaaaaaaac agctggggtt
    20041 gtttcttctt tacatgaaat ccctgcattg ttaaaataaa aatccatctc ttttcgtatc
    20101 ttattttaga taagatagaa aggagatgtt tttttgggaa aacgattgat caaagaagta
    20161 cctactagct ctttaccaag ggaacgtatg gaaatttatg gcgcggaagc tttatcggat
    20221 caggagcttt tggcaatctt actgcgtacc ggtcaacacc cttatagtgt gatgtcgatt
    20281 gctggaaatc tgttaaaaac gtttggagga ctggcctctt tacgacaggc tactttgcat
    20341 gagttagaag agattcgagg gataggacgc gtgaaagcaa tcgaaatcaa agccttgatc
    20401 gaattaggtc gacgaattca gacggatcat gaaagagcgg ctccaccggt tcgtaccagc
    20461 tacgaattgg cccagcaatt gattctggaa atgaaagatc acaaacaaga acatttggtt
    20521 tgtatttatt tggacacgaa gaatcaagtg atcttaaaaa aaacagtgtt catcggttcg
    20581 ctgaatcagt cagtcgctca tcctagagaa attttccatt atgctgtacg ttattgtgca
    20641 gcaaggatcg ttttagcgca taatcatcct tctggtaatg ttattccatc gcagcaggat
    20701 atgaatttta ccaaaaggat acaaaagtgt ggagaaatga tggggatcac tgtactggac
    20761 cacttgatca tcggacgcaa acgatatttt agtttgagag aagaaggaat gatggaagag
    20821 aagtaagttg ctttcttgca aaaaaaatga agcaagtgta caattatatt gtaattttgt
    20881 ttgggaatag aagttgaaac atctgttgca cctctatcgt acacgagcaa tgttacaatt
    20941 taaattagtg cttttggcac gaggaggata ttattatgga aaacggaaca gtaaaatggt
    21001 tcaacgctga aaaaggattt ggatttatct cacgtgaaga tggaagcgac gtattcgttc
    21061 acttctcagc tatccaaggt gacggattca aaacattaga agaaggtcaa gcagtgactt
    21121 tcgacgttga agaatcagac cgcggcccac aagctgcaaa cgttgtaaaa gcttaatttt
    21181 atctgtaaat caaattgatg gtagacatca atttttacga gagaacaaag cgagtaacaa
    21241 aaatacctgc cacaagagac aatttctctt gtggcaggta tttttttgta tgaaaaagta
    21301 aataaattta agaggctatg aaagagccac cttgtatcaa ggaataagaa caaacaaaaa
    21361 tggcttctca tgtttttcat ttgttttctc ttatattcag gacaaggtgc tgatttcata
    21421 acctcttatt tattcttcat ctggtgtcat taccattggc aaaatcattg gatgtctttc
    21481 tgtacgttcg aataagaaag gctgaagcgc gccagtcatc gcttctttca atttatattc
    21541 tgaacagtca ggtccattca aacattctct taaagcgtgg aataagattc gctgagcttc
    21601 attgatcatt tctcctgatt ctcgcatgta gacaaatcca cgagagagaa tatctggacc
    21661 agccaagatt tcttttttct tgatatccac tgtcgcgaca gctaatacaa gaccttcttc
    21721 tgaaagcagg tgacggtcac gtaaaacgac attaccaata tcaccgatcc cgctaccatc
    21781 gatatacaca tctccagcca caaaatgccc agcaggacgc gcagaatcgc tagttaacgc
    21841 taaaacatcg ccattttcca tgatgaagca gttttctgca ggaacaccta catcttgtgc
    21901 tagagaagca tgtactttca acatgcggaa ttctccgtgg acaggaataa aatattttgg
    21961 cttcatcaaa cgaagcatta atttttgttc ttcttgtcca ccgtgaccgg aagtatggat
    22021 gttattgatt tttccgtgaa tcacttctgc accagcttca gacagcaaat tgatcaaatg
    22081 attgacactt gttgtattac caggaatagg tgaacttgaa aagatcactg tatcgtctgg
    22141 ctggatttgg atttgacggt gagtaccatt cgcgatgcgg ctcaacgcgg ccatcgcttc
    22201 tccttgagaa cctgtacata ggatcatcgt ttcattggca ggcaagcgat tgatttcgtg
    22261 tgcatctaca aatgtgccgt ctggaacttt gatatatcct aaacgttgac cgttgacgat
    22321 ggcattttcc atgcttcgtc cgaaaacagc gattttacga ccagttgcca cagcggcatc
    22381 tgctgcttgc tgcaaacgga agatgttaga agcaaagctg gcaaaaatga tacgtccttc
    22441 gatttttgaa aagatcttca aaatagaatg cccaatcgtt ttttctgatt tagtaaatgt
    22501 tggaatctca gcatttgtac tatcagataa aaggcacaag actccttctt ccccgatacg
    22561 agccatttta tgcaagtttg ctggttcgcc aacaggagtg aaatcgaatt tgaagtctcc
    22621 agtcgctacg atatttccag aaggtgtttt caccacgatc cctaatgcat ctgggatact
    22681 atgggtcgtt ctgaaaaagc tgacagaagt ctttctaaaa cggatgatcg tatcttcatt
    22741 gatctcatgg agctctgcat ctcgaagcaa gccatgctca tctaatttgt ttgtgatcaa
    22801 cgctaatgcg agaggaccag catagatagg aatatttgct tgtttcagca agtagggtat
    22861 tccgccgatg tggtcttcgt gcccgtgagt aatcaccaag gcttttactt tgtgtaagtt
    22921 ttgtatgatg tagctgtagt cgggaatgac atagtcaatt ccaagtaaat cgtcttccgg
    22981 gaatttgatc ccggcatcaa tcaaaatgat ttcatcttgg aattgtaccc catacgtatt
    23041 tttcccgatt tcacctaatc caccaatagc aaagacgcct gtctcgttat ttttgatgga
    23101 aagtttcata ttaaaactcc actaatgaga aatcagcatt ttctttttcg tactctagat
    23161 gacttccttc tagaagttga acgaattcaa tattgtatgg ggtattttct tccacacgtt
    23221 ggcgagcgtc tacatcgctt tttgcctcaa tgtaaagaga ttttgtttct tcccgtttag
    23281 ggttgcgtac ttttgtttct tgataataaa ctttatagat cattgttttg tctcctttat
    23341 ctgcgtaaag cagtgattta gctagttgat ttttttagac aacacaaaac gaggggacaa
    23401 gccacctggt ttttcatatg ctttgactag aatactggat tcgagtcaaa gagacaagcc
    23461 tcgtttttgc ttatctgttc ggatattttt tcaaccgtac cttctgtata aaaaatatac
    23521 aacaattaat tgttattcta gcataaaaca gcaaataagt atagaaatca actagattga
    23581 aagaaaaaat caatgtttat catcccgcta taatcataat gactttttta tgagagaagt
    23641 gttctattaa taggctcgtt gtgctatcat ttttagtaat gaaaatgtaa caaaagatta
    23701 aaaggagatg agaaagtgaa actaatgtgg cattatacca tgcgttataa gaaattcttg
    23761 tttttcaatt tcatatgcgt atttggtttt attttgattg aattaggact tcctacgatt
    23821 ttagcacgta tgatcgatgt aggaatccgc aatgatgatt ttgattatgt gaagcaacaa
    23881 gggatattta tgatcgttat tactgtaatc ggtattattt tgaatatctt gttaggttat
    23941 tttggttcga ggattacgac taatatcgta gcagatattc gagatgattt gttcgaaaag
    24001 atccaaagtt attcgcatca ggaatacgaa aatctaggcg tatcttcatt gatcactcgt
    24061 acaacaaacg atgcttatca gatcatgttg ttcctgcaaa atattttacg tatcggtttt
    24121 atggcaccaa tgatgttcat tgtcagtttg tacatggtca tgcgtacaag tgtgacactc
    24181 agtttgtatg tagtaggtgc attaccttta ctattattgg cagtcgtagc gattgctaag
    24241 gtgtctgaac cgttatctaa aaagcaacaa aaaaacttag ataaaatcaa cagtatcttg
    24301 cgggaaaatc tttcaggatt gcgtgtgatt cgtgcattcg tcaatgaaaa gtttgaagaa
    24361 aaacgttttg ctgtagtcaa tgagaattat gcttctagtt caaaaagctt attccgtttg
    24421 atggctgttg cacaaccggg tttttatttc ttatttaata tcgtaatggt attgatcatc
    24481 tggagcgggg cagtgcaaat cgatgcaggc gagcttcgtg taggagattt gattgcgttt
    24541 atcgagtata tattccatgc cttgttctca ttcatgttat ttgctagtgt gttcatgatg
    24601 tatccaagag cagctgtttc agctggacgt atccaagaag ccctagatgc caaaccttcg
    24661 attgtagaag atccaaatgg tatatctgat acaaaaacta aagggtatat cgaatttcaa
    24721 aatgtaacat ttgcttatcc aggccattcg caagagccag tgatacgaaa tgtcagtttt
    24781 acagcctctc caggagagac cgttgctttt attggaagta ccggtagcgg aaaatctacg
    24841 ttgatccaat tgatccctcg cttttatgat gtgagtgaag gaaagatctt gatcgatgga
    24901 gtagacgtga gagattatcg gctaagcaag ttgcgagata agattgggtt tattccgcaa
    24961 aaagcgctgc tatttactgg aacgattgca gataatttgc gatacgggaa agaagatgct
    25021 acgcaagaag aaatggaacg tgcagctgag attgctcaag cttcagaatt catttctcga
    25081 aaaccagatg gatttaatga acatttatca gaaggtggtt ccaatttttc aggtggacaa
    25141 aaacaacgat tggcaatagc cagagccatc atacgccgtc cggaaatcta tatttttgat
    25201 gacagctttt ctgcattaga ttatcaaacg gatgcgaagt tacgtgctcg tctgaaaaaa
    25261 gaaacgacag aatctactgt attgatcgta gcacaacgtg tgggaacgat catgcatgca
    25321 gataagatca tcgtattaaa tgaaggagaa gtcgtcggta tggggacgca tcgggagttg
    25381 ttggaaaaat gcccgattta ttatgatatt gccgcttctc aattatcaaa ggaggaatta
    25441 gcatgaaaaa aaccttttct tccttaaaac gtttagctcg atatatcaga ccgtatcgtg
    25501 agacttttct tttagtgatc ctatttacag ttctgacagt ggctttcaac gtggctttgc
    25561 catatgttac aggacttcct actacagaaa tcagccgtaa tattgccaat ggcgaatcaa
    25621 tcaatttttc ttatatattc aaatgtctca tttggatcac agttgttgga atagggtatt
    25681 gtatttcgca attactttct ggtgttttga tgacaaatgt cgttcaaagt gccatgcatg
    25741 atttgagaag agacatagaa gaaaaaatca atcgtcttcc ggtttcatat ttcgataaaa
    25801 accagcaagg aaatattttg tctcgtgtga cgaatgatgt agatgcagtc agcggtgctt
    25861 tacaacaagc ttttattggt atcgtaaatg cgatattggg aattacgttg gctgcagcaa
    25921 tgatgtttta tatccagccg atgatggcgc tgatctctat gatcatgatt ccaacatcga
    25981 tctggatctc aaaacgagtg atcaatgctt cgcagaaata ttttcaaagc atgcagaatt
    26041 ctcttggtga gctaaacggc tacgtccaag aaaatatgac aggttttagt gtattgaaag
    26101 tatatggacg cgaaaaagaa acattggaag gattcaaaaa agttaatcac agcttgaaat
    26161 actttgggtt tcgtgcagca ttcatttctg gcttgatgat gcctctagtt caattaactg
    26221 cctatgctac ttatattggt atggctgttc taggaagctt ttatgttatt actggggcga
    26281 tcgttgtcgg tcaactacaa gcattcatcc aatatatttg gcaaatcagt cagccaatgg
    26341 ggaacgtgac acaattgtct tcagttttgc aaagtgcatc agcggcaacg aaacgtgtat
    26401 tcgagatttt agatgagcca gaagaaaaag aaaacaaagt agatgcttca ctgccagaaa
    26461 cgattttagg agatgtgaca ttcgatcatg tcgattttgc atatgatccg aagaaaccat
    26521 taatccaaga tttgaatttt gaagtgaaag ctggtcaaac cgtagctgtg gtcggtccga
    26581 ccggtgccgg caagacgaca ttgatcaatc tattgatgcg tttttatgac gtagacaaag
    26641 gagcaataag gattgatgga atcgacacca aatcaatgag tcgcagtgat gtgcgttcgc
    26701 tatttggtat ggtcttgcaa gatgcatggc tttatcaagg aacgattgca gataatatcc
    26761 gcttcggaaa gcttgatgcg acagattatg aagtagttga tgctgcgaaa acagcaaacg
    26821 tagatcattt tatccgaacg atgccagatg gatatgatat ggccatcaat tctgagggag
    26881 acaatgtgtc tttaggacaa aaacagttgc tgacgattgc ccgagcagtg gtctccgatc
    26941 ctaaaatctt gattttagat gaagcaacaa gttcagtaga cacccgttta gaagcgttga
    27001 ttcaaaaagc aatggatcgc gtgatggagg gacggacaag tttcgtcatc gctcaccgtt
    27061 tatcgacaat ccgtgatgca gatctgattc tagtgatgga tcaaggtcag atcattgaaa
    27121 aaggaacgca tgagagttta ttggcacaag gtggatttta tgaaaaactg tataacagcc
    27181 aatttgctga ggaagcagaa taagtcatat aagaataaaa cagtttaaaa cttatttttt
    27241 gttctctatt cttgataatg gtaaagtaaa gttatcaaga aaagtgagga cacggagatg
    27301 gaacagtttt taaatgaaaa aattactgaa gccaaaagag aagttgatcg tttaacgcac
    27361 gtctgtcaga cgaagccttt tgattcagct gatttttttg aaaatgagtt tcaaatcgaa
    27421 cgcttggcag cacaaataga agtgtacgaa ttaattttaa aaaaagtaat agaagaaaat
    27481 atagatgtac aataaaaaca ggtaaaacgt cagttaccag atactgtaaa tagacatctg
    27541 gcctga
//
LOCUS       DACDIG010000033        27342 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.033, whole genome shotgun sequence.
ACCESSION   DACDIG010000033 DACDIG010000000
VERSION     DACDIG010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.033"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            273..1751
                     /locus_tag="IV247_001495"
     CDS             273..1751
                     /locus_tag="IV247_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8777570.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            2028..4088
                     /locus_tag="IV247_001496"
     CDS             2028..4088
                     /locus_tag="IV247_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777571.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            4171..4740
                     /locus_tag="IV247_001497"
     CDS             4171..4740
                     /locus_tag="IV247_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8777572.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(4915..5721)
                     /locus_tag="IV247_001498"
     CDS             complement(4915..5721)
                     /locus_tag="IV247_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777573.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            5859..7373
                     /locus_tag="IV247_001499"
     CDS             5859..7373
                     /locus_tag="IV247_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAP8777574.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(7776..8759)
                     /locus_tag="IV247_001500"
     CDS             complement(7776..8759)
                     /locus_tag="IV247_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAP8777575.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(8764..9411)
                     /locus_tag="IV247_001501"
     CDS             complement(8764..9411)
                     /locus_tag="IV247_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8777576.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(9496..10128)
                     /locus_tag="IV247_001502"
     CDS             complement(9496..10128)
                     /locus_tag="IV247_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8777577.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(10144..10695)
                     /gene="coaC"
                     /locus_tag="IV247_001503"
     CDS             complement(10144..10695)
                     /gene="coaC"
                     /locus_tag="IV247_001503"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAP8777578.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(10692..11429)
                     /locus_tag="IV247_001504"
     CDS             complement(10692..11429)
                     /locus_tag="IV247_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAP8777579.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(11426..12415)
                     /locus_tag="IV247_001505"
     CDS             complement(11426..12415)
                     /locus_tag="IV247_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP8777580.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            12673..13215
                     /locus_tag="IV247_001506"
     CDS             12673..13215
                     /locus_tag="IV247_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8777581.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            13232..14317
                     /locus_tag="IV247_001507"
     CDS             13232..14317
                     /locus_tag="IV247_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8777582.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            14329..15135
                     /locus_tag="IV247_001508"
     CDS             14329..15135
                     /locus_tag="IV247_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8777583.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            15136..15963
                     /locus_tag="IV247_001509"
     CDS             15136..15963
                     /locus_tag="IV247_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8777584.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            15960..17033
                     /locus_tag="IV247_001510"
     CDS             15960..17033
                     /locus_tag="IV247_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8777585.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            17461..20157
                     /locus_tag="IV247_001511"
     CDS             17461..20157
                     /locus_tag="IV247_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8777586.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            20234..20722
                     /locus_tag="IV247_001512"
     CDS             20234..20722
                     /locus_tag="IV247_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8777587.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            20734..21228
                     /locus_tag="IV247_001513"
     CDS             20734..21228
                     /locus_tag="IV247_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8777588.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            21249..22058
                     /locus_tag="IV247_001514"
     CDS             21249..22058
                     /locus_tag="IV247_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8777589.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            22055..22876
                     /locus_tag="IV247_001515"
     CDS             22055..22876
                     /locus_tag="IV247_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8777590.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            22927..25026
                     /locus_tag="IV247_001516"
     CDS             22927..25026
                     /locus_tag="IV247_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAP8777591.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            25023..26141
                     /locus_tag="IV247_001517"
     CDS             25023..26141
                     /locus_tag="IV247_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP8777592.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            26180..27058
                     /locus_tag="IV247_001518"
     CDS             26180..27058
                     /locus_tag="IV247_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8777593.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            27204..>27342
                     /locus_tag="IV247_001519"
     CDS             27204..>27342
                     /locus_tag="IV247_001519"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777594.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CM"
BASE COUNT         8738 a         4700 c         5725 g         8179 t
ORIGIN      
        1 aaagagcagg acggtggccg tcctgctctt ttttgtggtt gtttacgcaa atggcgcaaa
       61 tcttgtacat tttaacacaa taaaacaaaa aatatagaat atttcctcat aaatttttcc
      121 attttttgca atattctcaa aaaaaagata aaaaatgaga ataactgatg aaaaaacaag
      181 cataatccat cataaaatca gtataggtga aaagatgaac tttcatctgc atatcaagta
      241 attggtatga ggcatttttt gaggtgtagg atatgaagtt ggattacctt atagaagagg
      301 aagatagaga aaaactgctg ctagctagat atttagaatt tgagaaaaag aattatgtag
      361 tgctgcctaa gttatatgaa acattaggtt tgtctaaatt taaaacaaga aattatatca
      421 tagaattaaa taaagaattt actgaattca aaacaaagcc agagatccaa atttcagaag
      481 aagcagagat ccatgtcgaa cgtctgaatt tgacggatat caaaaattta agaagaatct
      541 actttcaacg atcaaaaatt gctcgactgt taacggagat cgtggattca aaagtaacga
      601 tcgaaaaatt tgcagagaat cattttttaa gcatatcaag agcctatgta aaaagaagag
      661 aattagtctc ttttcttgaa cagcatcaaa taaagttaaa gaaaaacata ttagttgggg
      721 aagaaataaa catacggaac gtattatttg gggtatattt cggggtcttt aatggttttt
      781 ctatgccatt tagagaaagc gtcatgaatg aagcaaagag tatcgtgcaa tattttgaat
      841 atctgttcaa tttaaaactg tctgcaacaa aacgaatcaa attcaatttg ctaaccacta
      901 ttagtttgtt gagggcctat gggaatcaac caatcggaaa acgtttcttt tcagatgaat
      961 actttaagag ctcagaagga atgaagatca tcaaggattt atcacatatt tcttttcttc
     1021 atatccctaa agagcagatc attgatgaga tttcttatat catgttgttt ttgatcacgg
     1081 aagaagaatt tgaattagat ggcgcatttg gacttgcgga agctcatttt catgatatcc
     1141 acatgattag tcaaaaaatg tcagtggaat tgattgatca attaccattt tcgattgaaa
     1201 tctctgaaga agaaagacta tatatacgaa aagaacttcg tatgggtatg atgcatgtca
     1261 atcgaaaaaa ttggatattc gactttgaga tgactagctt tacgtctaaa aagcagctac
     1321 agtttttctt cgagacgtat ccaatattca gtacagcaat ccaaaaggtc atacaagaga
     1381 ataaacgcta tttcttggga aaagatgaac gtttcatgat tcgcttattt tatgattatc
     1441 tctttttatt ggtcggtatt tgtccggtat catattttga agcaccgatt catgtctgta
     1501 ttgatttttc gcaaggatct gcttataccg attacattat ttctcaagtg aaaggattta
     1561 aaaattacaa tattgtgatt gagagtcgaa tcacttctcg tacgaatatt tatttatctg
     1621 attgtttact tgaaaaattt aataaagaac agataatttg gaaaaatcca ccgactccag
     1681 atgactggga atattttgga aataccatca tcagagaaaa aaatagacat ttaactgaaa
     1741 aaatcagcta gtatttatgg atggcagctt tgtctcacat atttattagc tgatttatgt
     1801 ttttttcaaa gaaaaggtaa ctagatataa ctagatataa ctagatatat tacttaacaa
     1861 aattaaaaag tacataagtt attattccga ttttcttgaa acaataaaaa atcaaaaaaa
     1921 atactctcct aatagtccat cttcttttga ttttatttat ataatgaaat catcttatga
     1981 aaattactac ttcatgttta gacaagagta gggaggacta agaataaatg tgttcaaaaa
     2041 gacgttcaaa acaactaaat aaacacgctt gtcgtttaga taaggaacgg tcgtgttctg
     2101 aaaaacacta cgttaagttt atgatgcgca cgatgctgat cagtttatct gtaggtgtct
     2161 atatgcatgt acccgttcag gcaacgacac aaaccatcaa ggtcgtacgt acctctgttt
     2221 ctgagaccac tgattcccct atcactatta ctcatacttt cactgatgca aatggaaaac
     2281 agctacttcc tgcattgact tcctataata aagtcagtta tcctccaacg ataaaccact
     2341 actggttaat tcctaacaaa acattatgga catcggaaac cggaagcagt gtgccgcttt
     2401 ctatcttttc cgatatgact gatcctacaa aaggtgatca gttaaaacag atcactgata
     2461 ttttgaatca tgctttgaca gatataaagg acggtggaag cgcacaagtt tcttatgtat
     2521 atgaaaaaga ttattcacag tttgaaagaa agaaaatcga tgttctggca gggacaaaag
     2581 tttcgatatc tgatctggta gaaaggctga agaacgcaga tggaacacaa ggagatatgt
     2641 ccaaagtaat ttcaaacaat gggaatatcg tggatacgac taatacagga acatctacta
     2701 tttccttgtc ctattttgat ccaattgctt taaaaacaat gactgcatct gctgtgatcc
     2761 atgttcgtgc tgatcaaaca gctatcaaag gaaaagatct tcacttaaga acaggagaga
     2821 gttacgcccc taaagatctt gttgaatatg taacaaaaag cgatggaact tcaggaaata
     2881 aagaagaaat caaaatgaac gggacagtag atactacaac tccgggagaa tatcccgttg
     2941 tattaactta tatagactcg aaaacatcaa atgtggcaga aacaacagct gtagtttatg
     3001 tagaagcatc tgctgcttca tcgactgtca tcactcattt tatagatagt caaacaaata
     3061 aagaacttca agcagaaaca catatgagta gtgtaaatcg tattcctgat ttgcgcctac
     3121 ctgctgaatt ttctgatcga gtgccaaata tagatttatc gacttatgaa caaaatggga
     3181 aagtcacttc tatttctgat tggatgcaag caaacggatt agacagaaaa agtcactggt
     3241 cggagatcct tgaagtactt cactcagaac tgatgaataa taggaagcaa tcaggagggg
     3301 aaattacctt tacttatgtt tatataccag acaatcgttc attagaaggt catgatctta
     3361 ttgttcatcc aaacgaaaaa ataaacaaag aagagttgat ccaatcagca acaaattcct
     3421 taggtaaacc cgtaccgtta aaggatgtat cggttacaat caatgaagta ccattggagg
     3481 atggttattt gaccaaacaa acaggtgatt ttacgattat gtatagttat catgacccgt
     3541 attcactcca agaactccat gctcagtcac tattacatgt tttaccatta aacaaacaga
     3601 tagatcaaaa agaacaaaaa agacaagaag aaagcttgtc agaaaaaaaa caacaaatgg
     3661 cccatgcagt ttctccaaca gggaaagtaa aacaaacaag aagcctagag ttagcaagaa
     3721 tatctagaaa aatctcagaa tctaataaaa cgataaggca agaagctcat ttggccccac
     3781 aagtttctgg attggaaaat gaaattttag agcctaaaat aaaacaacaa tcagaaaaag
     3841 aatttgttgc taaacagtcg gaagatcaga aggaatcaga tgaccagaag gaaacgaaga
     3901 aaaaacgctt gtcaactatt gattccaccg atgagcatct gcagccgcaa gtaccacttc
     3961 ctagtccggg aacacccgca cctggtggag gtattccttc ttctgcttta ggagatttca
     4021 tgcgaggtat ggcaggaaca tgggtatacg ctaatcgaaa tgaatttgac gcagatgact
     4081 ggttctgatt tatatctcta tctgttatag tcaaataagt agctgagtca tatgttattt
     4141 ggcataatgg agacgaggag ggatagaaaa atgttagata aaacgttacc ttacgcagaa
     4201 atctggatga caagaccact tcaacaaaat gttccaatca cgcctttagc ggaaggtttt
     4261 gaatttcgat tttatcaaaa agaagatgaa tctgcttggg cggagattga gacttccgtt
     4321 ggggaatttc aaacgacaga tgaagcactt gagtattttc aacaaaactt tgctccttat
     4381 cctgaagaac ttgaaagaag gatgtttttt gtagagacaa aaaaaggaga aaaagtagca
     4441 acctgtaccg cttggcgaaa aaaatcaaga gagcaagtgt atccggtttt tcattggtta
     4501 gcagtgaaac cagattacca aggaaaaggg ctcgcaaaag cattgactgc cagagtcttg
     4561 caagaatttc ctaccctcca tacggacgga ccaatctatc ttcacacaca gacatggagc
     4621 catcaggcga ttgctcttta taaaaaaatg ggttttactt ttattgctga aaatctagac
     4681 ggcacaccta atccagatta tgaaaaagtt atgcaggttt taagtaaatt agataaataa
     4741 cgaaatggtg aaataagtcc tcagtattgg gataaaaaac gaaatagtca ttcctcatat
     4801 cttacttttt tgagttaata ctatcggata actaatggca tgccatacat tcaaaaaaga
     4861 aggggcagtg acaaaagtat tgtcactgcc ccttctcttg ttttttagca tgttttatcg
     4921 tttgattttt ttaggaataa aattcttgat gaacgtaaat ttttggtaat cgtttcgact
     4981 atagaggtaa ccaaatacac tgaaaacaac tgctccaata aaattaacaa ataaatcctt
     5041 catagtatcc atgattccca cgtctaagta accattttgg atgattgttt tatctgtttt
     5101 accatctgca ttttgacttt caatgattgt ccgagtgata tctggaacat gatagacagt
     5161 gtttccgtct ttacttaact ccacactatt gatttgctgg atgatcgtat ctttttgcat
     5221 atcgaattgc agtaatttat ctgctgaaaa ttccacaaac tcccacataa caccaactgt
     5281 catcgaaaaa cagaaggaaa caactgctag taataaagga gtcaagttca tggtttttgt
     5341 atttttattg agcaaataaa caagggaaaa gccgacaccc ccagctaaaa atccgctgaa
     5401 tgcatgtaga aaagtatccc acattgaaaa atagccataa aaatcactta actctcctaa
     5461 aactgttgaa ctaaaaataa aagataacaa aatgatttct agcatatttg gaatctgtat
     5521 ctttaatatc ttttccagaa aataagggat ttcaaataaa attaatgtca gtaataaaag
     5581 cattgtattt ccccagtgtt ggttccagac ctgatccact agcataataa cgacgatgat
     5641 acgtaataaa atatagattc cgtttgtgat tctacctgca gtttttgtac tgttcgtaaa
     5701 aaaggttttc aaatgattca taaaataact ccttcatgta tatccttaat tatactaaga
     5761 agcgtgtgtt taccaaagct atatgactta aagaaaaaca gagttgatat tgtgaaaaaa
     5821 atcacttaat atagtataag gagaaaaggg ggatacaaat gaaaaaatta gttgctggtt
     5881 tattaacagc cgttgcactt cttgctatga gcgcatgcgg cgcagatcag aaaaatacag
     5941 cgcagacaag ttctagtacg aaggaatctg ccgaagtatc atcaggcgct tctgaacaag
     6001 tatatacaga tccaaaagaa atgaaagaat cttatgatgt cgtgattatt ggagctggtg
     6061 gtgcagggat ggcagcagct atcgaagcaa aagatgctgg gttgaatcca gtaattttgg
     6121 aaaagatgcc tgttgccgga ggaaacacat ctaaatcatc aagcggtatg aatgcttcag
     6181 aaacaaaatt tcaaaaagaa gaaggaatca aagactcgaa tgatgccttc tatgaagaaa
     6241 cgctaaaagg tggtggagga acgaatgata aagaaatgct tcgatacttt gtcgatcact
     6301 ctgctgatgc ggtcgattgg ctagatcaaa acggtatcaa actggacaac ttaacgatca
     6361 ctggagggat gagtgaaaag aggacacatc gtccgtctga tggctcggct attggtggat
     6421 acttagtgga ggggttattg aataacgtcc atgagcggga aatccctatt tttgtcaacg
     6481 cagatgtgac agacattaag aaaaatgggg aaacggtcaa tgaagtgact gtacatgttc
     6541 aaggagaaga accaaaagag gtcaccggaa aagcaatcgt agttaccaca ggcggttttg
     6601 gtgccagcaa agaatttatt gaaaaatatc gtcccgattt agctgattat attacgacaa
     6661 atcaagaagg atcaactgga gatggtatca ccatgattga aaaattaggt ggacaaaccg
     6721 ttgatatgga taaaatacaa attcatccaa ctgtgcagca ggatgagggg gtcttgattg
     6781 gtgaggttgt tcgcggagaa ggagcaatct tggtcgatca agaggggaaa agatttgtca
     6841 atgaaatgga tacaagggat aaagtatctg cagcaattac agcattgcct gaaaaatcag
     6901 cttatttgat ttttgaccaa ggcgtgcgtg atcgtgccac agctattgaa ttttatgacc
     6961 aaaaaggata tgtcacaact ggaaagacaa tggaagaatt agcagaaaaa atcgatttac
     7021 caaaagaaac actagcaaaa acagttgatg gatggaatac ggatgttgcg gagaaaaaag
     7081 atgctcaatt caaccgtgcc acagccatgg aacatgactt atcgtctccg aattattatg
     7141 cgatcaaaat cgcaccagga atccattaca cgatgggcgg tgtgaaaatc aatacgaaca
     7201 ctgaagtatt agataaagaa aaccagccaa ttagggggtt atatgctgct ggtgaagtca
     7261 caggtggctt acatggaaac aaccggatcg gtggaaattc agttgcggag atcattattt
     7321 ttgggcgaca agcgggcata caagcagctc gatttgcttc ggaaaatgaa tgaaatagaa
     7381 cgtaaataaa aagaggttgt gaaatcagcg ttttgagcta ataccgaggg tcagctgatt
     7441 gaacactgtt tagtgaagag gttgtgaaaa agctgccctg catcaagtaa taagaacagc
     7501 caagtaaaaa cagcttctta tgtttttaac ttggttggac ttattaccgc agatgcaagc
     7561 ttttgaacac cgtttattcg gaaaaagagc gtgggacaaa agtaaagaac acttttgtct
     7621 cacgctctaa acacgtataa acggcggaag cagaagaaat tccttcggaa ataagctgaa
     7681 attcacaaaa atttgaaaag caattttcgt gaatttcttc ttatttcttg gagttaaacg
     7741 cttctgtccc agcctctttt ttgatcagca tgattttatt cagttatctt caaaatgatt
     7801 tttccgatgt ttttgtttgc ttccatatat tcatgcgctt gtttgactgc gctgaattgg
     7861 aatgtacggt caatgattgg ccgaatcttg ccattttcta gaaaaggccc agctttttgc
     7921 ataaaagttt gtgcgagctc tgctttatac tgatcacttc taggtgtcaa taatgtacca
     7981 agcagttgga cacgtttccc aatcaataca ccaaggtcta tctcaggcac gatggtccca
     8041 cctagcatac caatcaagac ccaacgtcca tcaacggcta tagaagctaa attcttttcc
     8101 cagtaagaag cgccgatgaa atccaaaata acatcgactc cccgaccttc agtgattttt
     8161 cgtacctctt cttgaaagtc ttgtttttta tagttgatca attcatctgc accaagttct
     8221 ttacaaaagg cgagtttttc ttctgatcct gcagtaacga tgattttcgc gtgagaaagc
     8281 tgtctagcta attggatcgc tgctgttccc acaccacttg ctcctgcatg gatcaagaca
     8341 gtttcgcctt cttgtaattt tccaagccag taaagtgttt gataagcagt caaaaaaact
     8401 tctgagattc cagcagcaga aacatagtct agcgtatcag ggagaaggat tgcacgatcg
     8461 gcaggcatca cagcatattc ggcataacca cctagattga ctaatccgta tacttttgtc
     8521 ccaggagtaa aagtggagat atcactttta ttctcaacta cctctccagc tacttcgact
     8581 cccaaaatag ggtagggagg tttcaaagag actgattctc ttttcataat atctgttcga
     8641 ttgacagctg ctgtatggac tttgatcagt aattcgcctt gttttggttt aggaacaggc
     8701 acttcaattt gttctaatac atctacagaa ccaggatgct tgattgaaat ggcacgcatg
     8761 gatctattcc tcctttgccc attcttggaa tgctgtattc aactctttgc taccttcaga
     8821 aagtactaga gtcgtacgag aaatatccgt ttcttccact agagcggcaa gtacagcagc
     8881 aactgtagag cgagtgatcg tttttgatgc cttctcatta ggcttagtta attgaatgga
     8941 agtaacttct tcatcgttag tcaatgtgac tggttgaaga atgacaaaat ctaagttagt
     9001 tcgtttcagc cattcatctg cataatgttt agtaatataa taatctgtca tggactcagg
     9061 ccattttgct ggttcatctg cataaacagc actgaccatc agatagcgag agacatgcgc
     9121 attttcagct gcgatcactg ttttgattgc gccatccaag tctacttgca gaagattctt
     9181 tccttgagag ccggcagcaa aaatcaatac atcgatgcct ttgaatgctt cggtcatttt
     9241 ttccacggac caagttaaat caaaagagac atacgaaaca ttctcttttt ttactaatgt
     9301 ttgggaagca acatctctca cccctgcaaa aatttcatgg gaacttggag ctaaatcttt
     9361 tatcaggtgt cggccaattt gtccgtttgc acctacaaca aaaattttca tttggatcat
     9421 cctttcgacg tcaaatcttt ccattcaata gtaacaccaa aagagaaaaa aatcagagat
     9481 cggctacgat gactatcagt tagtaagaga agggtgcttg aagtatctta acaagacatt
     9541 gcatactgct gcagtcgcaa gacctgcgac gattccttca ggtataccac ttgtaaaaat
     9601 aaccgctaga atagctgaat aaacagcatt taaatttgtt ccgatgactt tggcataatc
     9661 ttgctggaat aaaaagtaga tcatattcat cactaaaatc gtattggtca atgaaccgga
     9721 aagtccagct aagaataaag aaacggaatc tgctttttgt ttcattagtt tgcgcagacc
     9781 tttataaacg aaataaggaa ccaccccaat caagatgcga ggaataaacg cgatcagcaa
     9841 ggctttccag cttccatgtt ctgttccgat gactgggata aacggagaaa agacaaaaga
     9901 caaaggtgtt tggataactg tgctacggat catgctgatc agtccaaatg taccgccaag
     9961 aaaagcacct agtcttggtc ctaaaacgat cgaagcaatg attacaggaa tatgcatggt
    10021 tgttgcattg attggaccga tagggataaa acctaaaggc gtaacagcta ataaaatcaa
    10081 aatagctaaa aacatcgcag ttaatgtaaa gttttttgta tttttcataa gtactccttt
    10141 tttctaagca tttctttcat taagagtcag catgacctgt tcgatgatcg tctgaatatc
    10201 agctaaggca ccttttccaa aatctccaca agctaaaaga gattctcttg gttcaatttc
    10261 ctgatagccg acagatctca atgtttccag attacgctga gtgatcgggt tttgatacat
    10321 attcgtgttc atggcagggg caatcaattt tggtgttccc actggaagcg ccattgcgac
    10381 tgttgaaaga agatcgtctg caatgccgtt tgctagcttg ccgataatgt ttgctgtagc
    10441 aggcgcgatg agaaacaagt cagcttgttt tgctagttcg atatgattga ttacctgcgg
    10501 atttgattct tgcatgacgt ctgtatggac tgcgcgcttc gacagagatt gcaaggttaa
    10561 tggcgtgata aaatgtgtgc tgcttttaga cataatcaca tctacctggg cacctaattt
    10621 gacgaattga cttgtaagat cagcgctttt gtaagcagaa atacttccag taacgcctag
    10681 taagatcttt ttcattttga ttcctccagt ctaattttct cagtaatcaa tgcagcaatc
    10741 tcagatttac tttgagcttc ttccactgtt ccatctttag ataagagata gccgtggtgt
    10801 gtctcgccat ggatttccgt caaatcattt gcgaaaataa aatcagtttt gttatttatc
    10861 agactatttt ttgcgactgc cagaagagat tcctttggta catctactaa tagtttgaaa
    10921 ccaatgattt ttgcttttgg ctgccattca cgtagataag aaatgatttt tggtgttttc
    10981 ttcaaaacga gcacaagatg ctcggtgtta gaagagatct ttgaaacagt ggtatctgtc
    11041 tgttcatttt ccgcaaacca attgtccagt tcttcttgcg ttgaagaagt gggtaggttt
    11101 tttgccagct gttcttctga aaaactaaaa gcaggagtaa aatcactgac tgccatactg
    11161 tggataatcg catcgtagtg ttcttttttc gttagttctt ctaattgtaa aaataagtcc
    11221 agtgcagatt cgatcgtata aatcgttata ttggagtgtc ttttcggttt cagtgcttgt
    11281 ttcgtagtca cgtaatcgat tgtggtatcc ggattggcag ctaaataatc agctaatgct
    11341 tgccctaatc gtcctgtcga atgatttgta atagcacgta cttgatcgat tttttcagtc
    11401 gttcctccag ctgtgattag aatcttcatt tatggtcacc tacatttact agtgttcttc
    11461 cttggtgtgt tccagcaagt agtttagatg ctgtttcagg caattgatcc agagtaatct
    11521 cttggacgac cagttcatct gcaatctgca aatccgccat ctgttgccat aaagaaagac
    11581 gttgatctat tggtacattg acagaatcaa ttccaatcaa ctggatgttt cgcagaataa
    11641 aaggcaagac agtcgcgttt agtttgattc ccccagcatt gccacataaa gctactgcac
    11701 cgttataaga gatcaatgga agaagactgc ttagctgctc gccacctact gtgtcgatga
    11761 catagtcgat ttgctgtttt cctaaagctt ttgttgtctc tggcagaaat tcatcaggtc
    11821 gtacgacttg caaagcacct agacttttta gccatgtgac agactcttct tttctggaaa
    11881 aggcaatgat tgactggaat cctagtttat gtaacagggc tatcgctgta ctgcctacac
    11941 cgccagaagc accagtaacg acaacttttt tatcggaggt cataccttga cggattaaag
    12001 cgttcacaca caacatagca gtaaaccctg ctgtaccaag gatcatcgct tgacgcagcg
    12061 tcatattttt tggtagagga actaaccagt ctcctggtac tttttgatat tggctataac
    12121 cgcctggatg ggtgacgcca agcccaaaac cagtagcaag tacaagatct tcttttgaaa
    12181 aacgtggatt gttactttgg acgacagttc cggataaatc aatccctgga gtcatcggat
    12241 atgttcgtat cacctgacca gactcgctca ttgccaatgc atctttataa ttgacatcag
    12301 aataggcaat ttttaccaag acttcatttt cagccaactc agtaaatggc agatcactga
    12361 ttgctgtatg gaaaacaggt tcctttatta cctgaaaaga tcgaaaagcg ttcatttgtg
    12421 agacactcct tttaaaattt tcttttatca taccataaag cctactcttt tattcttgtt
    12481 cttccgtaaa tttagacaaa aattaggtga aaaataaaga atcaaacaac tagaatttat
    12541 tccaaaagat aaagaaatat tcagcaaaaa aaattttaca aatggttgcc tttttcaata
    12601 aatttcgtat actaatacga gtggtagtaa acaggaagtt tactattaga aaacttcgat
    12661 tcggaggaaa ttatggaaat tggtgagaag ttaagaaatt tgcgcatcca aaagaatttg
    12721 acacaagaag aactaggcga acgcacagat ttgtcaaaag ggtatatttc ccagttggaa
    12781 cgagatttaa gttcaccatc aatggaaaca ttcttttcta ttcttgaagt cttaggtgtg
    12841 actccagaag aattttttca tcaagaagca gcgaatctgc aagtggttta ttcaaaagaa
    12901 gatcatacgg tttatttcga tgaagaaaac gggtatgaat tggaatggct cgtggctgat
    12961 tcgaatgaaa aagaaatgga gcctgtcttg ctgacttttg aaggtgcagg agaatataag
    13021 acgtttgaac cttcattatc tgaaacgttt atttttgtat tagaagggga attagagtta
    13081 agcttaggag agacggtcta tgtggcaaaa gcaggacagt cgatctatta tcaagcgaca
    13141 aaacaacatc aattaaggaa tcatctcaaa aaacgaacaa aagtattgat tgttgcaacg
    13201 caatcatatt tgtaatatag ggggtaaaaa cgtggggaaa ccaatcattt catttgatca
    13261 agtagtgaaa cgctatgatg atgaaacagt attaaaaaaa gtaagttttg agatcgaaca
    13321 aggaaaattt tatacactgc ttggtccttc cggctgtggt aaaacaacga tcctacgtat
    13381 tatagctgga ttcaccgaag ctacagaagg ggacatctat tttgaaggga aacggttgaa
    13441 tgatttgcca gctaataagc gacaagtaaa tacagtcttc caagattatg cactttttcc
    13501 tcatatgaat gtttttgata acgttgcttt tggattgaag atcaagaaaa tgtccaaatc
    13561 agatatcgaa aagaaggtaa aagatgcttt gcgtatggtc cagcttcctg gatacgaaaa
    13621 acgggagatt agtgaaatgt ctggtggtca aagacagcgt gtagcgattg cacgagcaat
    13681 tgtaaatgaa ccgaaagtat tgctattaga tgaaccgtta tcggcgcttg atttgaaact
    13741 acgtacagat atgcagtatg aactgcggga tctgcaacaa cgattaggaa tcacgtttat
    13801 ttttgttact catgaccaag aagaagcgtt ggcaatgagt gatgagattt ttgtcatgaa
    13861 taaagggaaa atcgtccaaa gtggtacacc cgtagatatt tatgatgagc caatcaatca
    13921 tttcgttgct gattttgttg gggagagcaa tatcgtggac ggtgtaatga tcgaagataa
    13981 tctagttgag tttgttggta agcaatttga atgtgtcgat ggcgggatgc gtctaaatga
    14041 accagttgaa gttgttttac gcccagagga tttgacgatc acgacaccag ataaaggaaa
    14101 actagtcgta acggtcgata ctcagctgtt tcgtggtgtc cattatgaaa tcatctgtta
    14161 tgatgaacag caaaatgaat ggatggtcca ttcaacgaaa aaagctaaag aaggaagcaa
    14221 agtaggattg gcctttgagc cagaagatat ccatgtcatg cgtttcaacg agtctgaaga
    14281 agaattcgat gcacgtcttg acagctacga agaatagggg ggacaagaat gaaaacaatg
    14341 cgcaggatct attccgtccc ttatattttg tggctcggtt tattcgtgat cgcgcctgta
    14401 ttaatgatca tttatcaatc tttttttgat atgaatgggc aattcacgct gaataattat
    14461 ctatcttatt ttacgtcggg gacatcgctt tctatgacga tcaattctgt atggtatgca
    14521 tttttgatca cgtttttcac gctgatcatc agttacccga ctgcttattt tttaacaaaa
    14581 ctgaagcaca agcaattatg gctgatgctg attattttgc ctacatgggt gaatctgctg
    14641 cttaaagctt acgcatttat tgggattttc agtgtgcacg gcagcgtgaa tcatttcttt
    14701 gattttatcg ggatcgggcc gaaacagata ttattcacag acttcagttt tctgttcgtg
    14761 gcaacgtata tcgagattcc atttatgatt atgccaattt tcaatgcatt ggaagagatg
    14821 aatccttctt tgatcagtgc tagtagagat ttaggcgcga atagctttga gaccttccga
    14881 cgagtgattt ttccgttatc attaaatggt gtgaaaagtg gtgtccaagc agtatttatt
    14941 ccttctcttt ctttattcat gctgactcgt ctgattggcg gaaatcgggt gatcacttta
    15001 ggaacggcta tcgaacagca tttcttggtt acacaaaact gggggatggg ttctacaatc
    15061 ggtgtgattt taatcattgc tatgttcatc gtgatgctcc tgacaggcga gaagaaaaaa
    15121 ggaggtcgct cataaatgaa aaaatttaaa tggtcgtatc tttatttagg gatcgtcttt
    15181 ttattcttat atgcaccgat cttctatctg attttttatt ccttcaatga aggaggaacg
    15241 atgaacagct ttacaggatt tacttgggaa aattatgaag ctgtttttga agatacacgt
    15301 ttgatcatta tcgtattgaa tacgttcatg ctggctttct tatctgcatt gatcgccacg
    15361 atcatcggga catttggggc tatgggaatc tattacacga aaaaacgacg tgcacggaca
    15421 gcgttgttga gcatgaataa catcttatta gtctctcctg atgttattat cggtgctagt
    15481 ttcttgattt tctttacact actaggattc ggattaggtt ttcagagtgt attgctttcc
    15541 catattgctt tcagcattcc aatcgtcgtc ttgatggtct tgccaaaatt gcaggaaatg
    15601 aacgattcaa tggtcgatgc ggctagagac cttggtgcta ataatttcca agtagtcaaa
    15661 aatatcattt tgccttatct ttctccagga atcatagctg gatattttat ggcatttacc
    15721 tattctcttg atgattttgc ggttacattt ttcgtcacag gaaatggctt tacgacgctc
    15781 tcagtagaga tctattctcg tgcaagacaa ggaatcagtt tagaaatcaa tgcgctaagt
    15841 gcgttagttt tcttgttctc catgatattg gtcattggat actactttat tagtaaagaa
    15901 aaccgtccaa aacggctgaa gaaaaacaga agagtacgcg agggggtggc acggctgcga
    15961 tgaaaaaatt acaatctctg tttttaggta ttctcatcat tattgcggtg ctgctgcttg
    16021 gtgttcgtca acttgaaaaa acaagtggca tgagtggagc aaaggtactt accatctata
    16081 actggggaga ttatatcgat cctagtttga tcacgaaatt tgaaaaagaa tatggataca
    16141 aagtgaatta tgaaacattt gattctaatg aagcgatgtt cacaaaaatc cagcaaggtg
    16201 ggacaaacta cgacatcgct ataccaagtg aatatatgat ccaaaaaatg atcaaagaaa
    16261 aactagtact tccattagat cattcgaaga tcaaaggatt aaataatatt gatccacgat
    16321 ttttagactt ggattttgat ccagataatc agtattcgat tccatatttt tggggaactt
    16381 tagggatcgt atataacgat aaattctttg atgcaaatca aattaagcat tgggatgatt
    16441 tgtggaagcc ggaattaaaa gacagtttga tgctgattga cggtgcacga gaagttatgg
    16501 ggctttcttt gaatagttta ggctattctt tgaatagtaa gaatatggca cagctgacag
    16561 aagcagcaaa taaattgaat catttaacac caaatgtcaa agcaatcgtg gcagatgaga
    16621 tcaaaatgta tatgatcaat gaagaagcct ccgtggcagt gacattttct ggcgaagctg
    16681 ccgatatgat gtgggaaaat gaacatttac attatgtgat cccaccagaa ggatccaatc
    16741 tttggtttga taatatcgtt atgccaaaaa ccgtgaaaaa taaagaaggt gcctatgatt
    16801 tcatcaactt tatgctagaa ccggaaaatg cggcacaaaa tgcagagtat attggttatt
    16861 ctacaccaaa tgaagaagca aaagcgttat tgccaaaaga catttcgtcg gacgaacaat
    16921 tctatccatc tgatgacacg atctcccatc ttgaagtcta caaagatcta ggatcgaaat
    16981 acctaggcat ctataatgac ctattcttag aattcaagat gtataggaga taatggcaaa
    17041 actcagtaag aatggggact tatcaacgtt ctaaagagct aaaaatcgtc ctttttctat
    17101 ctgagttttc caatcttcta ccaaaagcgg atgaaacgcc gctttttttg tgttatttta
    17161 ttttttctac cacgtttttc taccagagaa aagtggacaa aaccattgta aaatagggcc
    17221 cgagacgggc tagtaaatct aagattgaaa aagttacaga aggataaagt aattggaaac
    17281 tatgcaaata tacttaaccc ttctgtaatc tattaataaa actaatatat gaatccagtt
    17341 caatatttgt agctatgaaa aaacataaaa atcatattag ataaaaacta aattggtaga
    17401 taaattattt tgtcagatat ctgaggcaaa tattcgacta tttgtgtttc aaagtaaaaa
    17461 ttggaataca aatatagaat agacatattg cagccgatag tttattatgc ttttagacac
    17521 attaaactgg aggaaaaaat gaaaaagagc gctaaagatc gagtttttga tgagctgata
    17581 cgactaacag ggaatatgtc ttctgtcact gccgagaaaa tttcgaagaa tctaggtatc
    17641 tctcgtcaga atgccagcca ttatttgacg agattagtgg aagataaaaa ggtcgagaaa
    17701 ttaccgggaa agcctgtttt gtggaagcct ttagatgaat atgcggttgt agataatacg
    17761 gagcaaataa acgaagcctt tcattctgta gttggtcatg acggaagtct gagagaggtc
    17821 attcaaaaat gtatagccgc tgtcaaatat ccgcctaacg gactcagtgt attaataaat
    17881 ggagccacgg gtgttggaaa aagtttttta gccacaaaaa tttttgaata cgctatccat
    17941 gagcaaatta ttgaaaaaga tgcaccattt gctatcttaa actgtgccga ttatgcggac
    18001 aatcccgaac tgctttcggc aacactgttt ggatacaaga agggggcctt tacgggggct
    18061 gaaaaggata ccgatggttt attggcaacg gccaacaacg gctatctttt tctcgatgag
    18121 gtccatcgcc tatcgaaaga aaatcaagaa aagctctttc tatttattga tactggaaac
    18181 tatcgtccag tcggtgaaaa tgtaaattgg cattctgcta aggttcgttt tattttcgcc
    18241 acgacggaaa aaggtgaaaa ttatttatta gacacctttg atcggcgtat tcaaattagt
    18301 gttatgttgc caacttttga agatcgcccc attcgagaac gattagagct gattcaatta
    18361 ttttttcaaa atgaagcgga tgttttacaa aaagatatta ttgtttcaag agaagccttg
    18421 tctttaatgt tatctcatcc ctttagtggg aatattggta agctgaagaa tatcattaaa
    18481 atcagttgtg cggatgtgta tagtcgaaca caagaagaac cattaacgat tgggaaaaat
    18541 gagatcttga ttcagttaaa tatgctggaa tccgaagtag aaagcttgcc gattgctagt
    18601 ttattgataa agaaaaatca aaagataaaa acggatcgaa tcaaaccgtt tgattatgat
    18661 tcgattttgg aaaaaattac tcagctgata aatgaaggta atttcaatga aaatttttct
    18721 tcgataaaga atgaagtaca gcatttggtg catcacattg ataaggattc ttttttatca
    18781 atgagtagaa aattgatgat tcagatgttt agaaaggtct gggtatccgt tttaggaaaa
    18841 acgtacggat tgacgacgag tggacctgtt gcagaaatta ttagcagatc ctatgtcatt
    18901 tccccaagct ataagacaga cttaaccggg ataaatcgta cattagctgc aaatctacct
    18961 cggacgactt acttatgtaa tcagttcttg gagcatttac caccgctctc agaaaaggac
    19021 gagaactttc taagactgct tattgtcgtg actttatctg attatgtaga tgaatccatc
    19081 gagctgaagg gcctgttgct tgctcacgga gatagtacgg ctagcagcat tcaagcagta
    19141 gtgaatcaat tatgcggcaa ttatgttttt gaagcgttgg atatgccagt agagagtaat
    19201 gtgtcagaga ttgtcgaatc gacaaggaat ttcgtgaaac ggcaagtgaa tacagatagt
    19261 ctagtgctga tcgtagatat gggctcttta aatcaaattt acacacagat caagaacgat
    19321 ttaaaagggg agttacttct tctgaataat ttgacgactt ccattgcact tgatattggc
    19381 ttgaagatga cgatgaatgc gccatttaaa cagattgcgg aggatgcagc agacaagtat
    19441 tcaatcaatg ttcaatactt tgaaggcttt tctcaaagta gcaacattat catttcctgt
    19501 atgtcgggtt tggggatttc ggacaaattg aaagatgttt ttcgtcatca tttatcggat
    19561 gaatccatcg aagttttcac gaaggactac agagaactac gtgaattaat cgaccaaaat
    19621 gatgaaactt attttgacaa gacaaaattg gtcatcacga cttcggatct tccacgttcc
    19681 ttttcaattc ctaatattaa tgtttatgac atcttagacg gtgatggagc agccaatttg
    19741 gagcatattt tgagtcctga aatgaagccg gcagaattta accgcttgat tcaggagcta
    19801 gtaaaattct tttcgttgga aggaattgcg gaccgtttga actttctgaa cccaacgatc
    19861 gtgatgagtg aagtggaaac agtcatttct aaatatgaaa attactatca tttcacttta
    19921 aatggccgtg taaaactaaa tctctatatg catacggcat tgatgatgga acgtttattc
    19981 ttggctcgga cagaaaaatc tgaagaaccg aattatgatt tgaaaccaga tgaagatgaa
    20041 ttttattatg tttctagaag tatttttcaa tcaatggaga tgaaatataa ttttaaagtc
    20101 aatgcttatg agctttcgct gctctatgaa ttattaggac cgtatattca aaaataaagt
    20161 gtctaaaaaa gtgtctaatc tgtttttttt aggttagaca ctttttttaa tgtgaattaa
    20221 cgtgattaaa ccaatgtttt taaaagttgg cacaggaatt gcttatataa aagtgaacag
    20281 aaagaaagga attaaccata tgaagaaaat tctaatagcg actcacggtc acttagcttc
    20341 aggctttctt agcagtattc aattactgac ggggaaggtt caagagatta cggtgattaa
    20401 tgcttatgtg gatgagtgtg attttgaaca ggagttgaat gtatttgtaa accaggtgaa
    20461 cgagcaggat cagatttttg tttttaccga tttatttggc ggaagtgtca atcagaagat
    20521 taccaaaacc tttttggaga aagagattgc tgtaactatt ttgtctggct ttaatctgcc
    20581 aattctgctc gaaatagtat tggcaaccaa ggaattaaca aaggaggata ttcaggaact
    20641 ggtaaataaa tgccaacagg aattaaagat gaccattctt gatgaggtac atgtagtaaa
    20701 tgaagaggaa gatttttttt aggaggaaga aaaatgatta aagcagtgcg aattgatgaa
    20761 cgattaattc atggacaagt agcgatgact tggacaaaga gtctaaattt aaccgggtta
    20821 gtagtagcaa gcgatgaggc ggctaccaac gatattcaaa agatgacatt gaagatggct
    20881 gcaccatcga atgtcaaagt cattattaaa acagttgatg acgcgattca tttgttaaaa
    20941 gatccgcggg cgaaggacat gcgcttgatg gttttagtcc ctaatgtcaa agatgcggta
    21001 aaagttgcga aagctttccc aacggaaatc gaattaatga acgtggggaa tgctgggaaa
    21061 atgacaccgc catcggaaga taagaaaacc ttatcaaagg aagtgatgtt aacgacgtca
    21121 gaaattcaag acttaaaaga attagttgaa ctttatccag ataccgtttt tcagcctaca
    21181 ccggcgatgg aaaaacgttc agctatctct attttgagtg atttttagaa aaaggaagga
    21241 gaaaaattat gtatgatgca tttgtagtcg ctttggccgt ttttattgga gttgctggac
    21301 atgaaatgtt cgggatgtcc atgctgagtc gtccgattgt tgttgctccg ttagcaggag
    21361 cgttgatggg ggatttgcag acggggttga ttgtcggggc gtcactagaa gccatcttta
    21421 tgggtgtcgt caatatcgga atctctagta cggcggagcc ttctttggct gctgggctgg
    21481 ctacagcgtt cgcaatcaaa atgggaggaa acgtggatgc gattattccc attgtctttc
    21541 cactagcagt attaggatta cagttaatga atatgatttt ttcttttgta tgtggaccga
    21601 tggctgctaa atttttcaaa tttgcaaaat ctggtgatca aaaaggaatc atcaatctgc
    21661 acttctttat gtggtttgtc cactatgcgc tttatgctct tattccattc tttgccgttt
    21721 tatttggagc agatgttgtc aaaagcgtct tggataatat tccagaggtc atcatgaacg
    21781 gattaacagt cgctggaaat ctgctgccgg cagttgggat ggcgatgctg ttacggatgc
    21841 tttggagtaa aaatattgct gtgtggtttt tctttggggc cgttgttatg gcgtatctcg
    21901 agttaccatt gattgcaatt gccgcagttg gaacgattat tgcaattgtt atagcccaaa
    21961 gagatctaca actaaaaaat atatctgttc atgcagttca aagcgatgct gctgtaaatg
    22021 caacgcttga ccaagaagag gaggatttct ttcaatgaaa ttagctgatg gaatttctaa
    22081 agatgagaaa aaattggttc ggaagatgtt ttggcgctcg atgactatgt atgcttcggt
    22141 gaatccggtg acgatgggcg gcggtggttt cgcctattct atgatgccct tcattaatca
    22201 tttctacaaa agtgaagaag atcgagctgc ggcgctggaa cgtcatacac agtatttttc
    22261 gacgaccatt ccttgcgcgt cctttgtcat ggggattgcg gcatcgatgg agaaggaaaa
    22321 ttctgaaaaa gctggttttg acgtggaaag tatcaacgcg atcaaaacga gtttgatggg
    22381 accattggct ggaattggcg attcattgtt ctggggagtt tggcgtgtcg ttaccgcggg
    22441 actagcgatc aacttagcca atgccggaaa tattttggca ccaattatct ttttagttat
    22501 gttcaatata cctaattact tgatgcgata ctatggcggc gttttaggct attcattagg
    22561 ctctaaatat attgaaaagc tgtatgcgaa cgggttaatt gaaattctga caaaagctgc
    22621 tagtatcttg ggtctgatca tggtaggtgc aatgacgatc agtatggtca aattcaatac
    22681 cgttttgagc ttttcaatga agggcgaaga actaatgaat gtccaagaaa tcctcgatag
    22741 tatttttaaa ggaattgtac cgttattggt aacattgagc tgtttcaaat tactagataa
    22801 aaaagtcggc gtcatcaaaa ttatttttgg aatggtcgtc ttgggaattt tattgtcatt
    22861 acttggaatc gcatagtagg aagctgcagc tactaggctg cagttttttt agcaaggagg
    22921 gaaaagatga ctattaatga agtaatttat tcacccaaac acccattttt taatgctgag
    22981 ttaaacgacg gaaatgtgac tacttcaaac gaatacagct actatcggaa tattcctgat
    23041 gtaaagggat tgaccttcga tgtaagtaat caaaagacat tagcaaacct tgatgtttgc
    23101 gggacgattc aaacgttgac cttttatcga gaaaatttac taactgagga aaaaccaggt
    23161 gtttgggtca ataaacagct ctctcaaacc aattcgttat ccttaaaggt tgaagtaaac
    23221 gggattgtct accatttgtc agaatcggat catcgtattg aagtcgatgt gattcaagac
    23281 tgtttgccta gatatattca tcattattcg acgtttaagg tcattgtagt atcctttgct
    23341 cccatactgg ctaataagcg tttaagtatg cttgttcaac aggtttacat tgtgaatgaa
    23401 acacacgaaa gccttcaagt ttcagtgaaa gatgtgcctt cgtatcaaga gaagtattcg
    23461 gatcagcaaa atgtgttgat ttctcaaaaa ggaaataagg acacgatcgc acaacaggaa
    23521 gtggcaagct tcgccattgc ttttgtggac ccaaatgctt ttgaagaagt aatgactttt
    23581 caggaatctg atatcgaaaa atggctgaag gaaacattga actattttgc tcagctattt
    23641 gggcagctgt caatgccaaa cgatagagtt gtccatctct atcgtcgtgc actttatcaa
    23701 agcttttcta gtttcggtat gaatcgacag ggacaggtcg tcggcagcaa ttggggcagc
    23761 tatcctgcta ctaatcgaat ttggaataag gatatgtatt acgcatcatt gccattcacc
    23821 atgtttgaac cggagttatg tcaaaaaacg gttctatggt ttgatcaata tggcgttaag
    23881 tttcctggtt caaaatttac aggaggtatc aatcattctc tgagtaattc gttatccagt
    23941 gcgttactgg ctagtcttta ttttgaacat actggggata caaaattctt tgagcggtgt
    24001 ccgcaggttc tactgaatgc ttcaagaatt attgaggaca tattagctca acgtcggtca
    24061 ggggaaccaa tgctttttcc atctgtttgg ttatcagacg cctttgcact tggaaagtat
    24121 cataccggca gcaatctttg tctttggaag gcctgtattg gacttgcaga aatttttgaa
    24181 gccctagaac aactgagtct tagcaagcga tacaaaaaca ttgcctgtaa actaaaggaa
    24241 tcaatttgta aacaaatgac tatcgatggt ccttttggtg agcaattttt ggagggaatc
    24301 ggtgatgagg agaaaacaac ctattcagtg aagcactatc aaaaaccgat tcttgagcaa
    24361 gggttaatct ttttaagtga tgtgattgtg gatggaaaaa ttaatttatt gatgcatgat
    24421 ggagaagaat cagacactac gttgatgcct ttttatcaat ttttagataa taaagatcat
    24481 ttatatcaga acaccatggc cttttctgct agttctttca atccaaccta tagcgaggaa
    24541 atcaaaggca tcacttgggg attagaatcc ggcgccactt ttcctggctt tatcactgtt
    24601 ttaatgagcg agctgcacaa ccctcaagct ttcgtaaccc gattagaaga actaatttgt
    24661 ttggcagatt tagacggttc gtggtggtgg tggccgtatc gcttagaggc tcaacaagga
    24721 gaagtcgtgc gagactttgg ctgcggcaaa tgcggctggg catcgggtct ttttgtcagc
    24781 ctaatgatca gtcaatattt cggattgaaa ttcaaaaaag gagtattgca aattgatcct
    24841 gttgataatt taacgttttc ttggaagaat ttgaagttcg gtcaagcctt catttcgatt
    24901 gaatgtacgc aatccgagtt gagtatttca aatttgtcag aaaagccatt gaaaataagt
    24961 gatacgaaaa aaattcttca cgtagaaaag ggaaaaacga ttcacattca aaggagaaaa
    25021 agatgagtat actaaaaaat attacccaat ttaaaaaaat ccagagtcga gcaatcactc
    25081 ccgaaaatcc aacaggcgag aaaggtaaag ctgcgatgtt gccaagcgag ctaggaccaa
    25141 gccgcaaagg tcgtggcgca attccgctga cttctggtga agaaactgtg attgcggata
    25201 ttactggaac gggaataatt cgtcatatgt ggatgacgat tcgtgatcga acagaatttg
    25261 gcagttttgt tttacgtgat ttaatcttaa gaatttattg ggatgggtcg gatagccctg
    25321 ctgttgaaag tcctttaggg gatttctttt gtaatgggtt tggagagcgt tgcgaggtga
    25381 actcgttgcc gatagtggtt aatcctacag ggggctttaa ttcctatttc gaaatgcctt
    25441 tccaaacatc cgctaaaatt acgatcgaga atttacatcc gaaggatatg tcctcatttt
    25501 tttatacgat aaattacgct ttggtggacg aattggaaga aaatagccta tattttcacg
    25561 caaattggca tcgggaacgc attaccaaat tacaggagga ttataagtta attgatcgat
    25621 taaagggtca aggctattac gtgggtactt accttgcgct tacctgccta gaacgttatt
    25681 ggtggggcga aggcgagttt aaattctaca ttgatgggga tagtgagtat ccgaccgtaa
    25741 cctcaacagg ttcagaagat tattttggcg gggcgtgggc ctttcatgag tacgatgagt
    25801 tcggacgtcc acatgcgaaa aactattcaa cagccttttt agggtatcct taccaatcaa
    25861 atagagatgc cacaagagat cgttttgaaa cgggaaaatt gaatgccgtt catgcctttg
    25921 gtgacgacgc aatgccacga catggtcttt atcgttggca tcttcaagac cccatctgtt
    25981 ttaccgaaga tttatcggtc actttacaac aaatcggcaa cgatgatgtt cgcttatttg
    26041 agcggcaaga cgatgttgct agtgtcgctt actggtattc aacagaagct tcaggaaata
    26101 ctaaacgtca tttcactgat cgagaacgct tgccgcgtta atgaagtgtt tttcacatat
    26161 tcgaagggag gaacctatga tgatatatgg ggcaattgaa gctggaggga ctaaatttgt
    26221 ctgtgcagtc ggtactgagg aattaacgat tttggaaaga gtcagttttc ccacaacaac
    26281 tccagttgaa acaatgaagc aggtcattga tttttttaaa ccttttaagg aacaattggc
    26341 aggcatcggg gtcggctgct ttggtccaat tgatattcaa tgcaaatcaa cgagtttcgg
    26401 tcatatcaca tcaacaccaa aacttgcttg gcaaaatttc aattttgtag gaacgctaaa
    26461 gcaatatttc aatattccga ttgaatggac aacagatgtc aatgcggctt gctatggtga
    26521 atatgttgct ggttctggca aagggttagc aggtgttgca tacttcactg tgggaacagg
    26581 tatcggaggt ggtgccttgt tcaatgggac tttcgtggaa gggttcagtc atccggaaat
    26641 ggggcatatg ttgatcaagc gacatccaaa ggacaatttt tcaggaaact gtcccttcca
    26701 ccaagattgt ttagagggga tggcagcagg accagcgatt gaaaaacggt tgggtgtaaa
    26761 aggccagaat ttattggctg acgattcgtt ttggcagatt gaagcctttt accttgccca
    26821 atgtgcatac aatacaacac tgatgttttc gccagataga attatttttg gcggtggtgt
    26881 aatgaaacaa gaacacatga agaaaaaagt tcaggataaa tttgttgagc ttataaatgg
    26941 atatgttgaa ataccaccaa ttgatagcta cattattacg cccgaattag gtgataatgc
    27001 cggaataatc ggcggattag cattggccag aaaagcggta agaaataagc aaccataatt
    27061 aatgaatgtt tcattgatac tcatgtcaaa agtatttaac cgagtaatca aatgatcaaa
    27121 tgaagttgga taaagcgaaa atgaattcaa ataaaaaggc tctaagtcaa atcgtgttgt
    27181 tttcattaaa tcactgaaag gaaatgcttc tgcgtttcct tttttctttt tgttgtgcgc
    27241 tgtatacctt gaggatattc actggtggcg tacacttaaa tagtaatata ttgtgttact
    27301 ttatgtctgt ggtaatagtt atagttgctt accaatgtat gt
//
LOCUS       DACDIG010000034        27111 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.034, whole genome shotgun sequence.
ACCESSION   DACDIG010000034 DACDIG010000000
VERSION     DACDIG010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27111
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.034"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1554)
                     /locus_tag="IV247_001520"
     CDS             complement(<1..1554)
                     /locus_tag="IV247_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAP8777595.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDG"
     gene            complement(1558..1968)
                     /locus_tag="IV247_001521"
     CDS             complement(1558..1968)
                     /locus_tag="IV247_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAP8777596.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(1965..2888)
                     /locus_tag="IV247_001522"
     CDS             complement(1965..2888)
                     /locus_tag="IV247_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAP8777597.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(2885..3763)
                     /locus_tag="IV247_001523"
     CDS             complement(2885..3763)
                     /locus_tag="IV247_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP8777598.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(3750..5087)
                     /locus_tag="IV247_001524"
     CDS             complement(3750..5087)
                     /locus_tag="IV247_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP8777599.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(5090..6193)
                     /locus_tag="IV247_001525"
     CDS             complement(5090..6193)
                     /locus_tag="IV247_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP8777600.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(6267..7775)
                     /locus_tag="IV247_001526"
     CDS             complement(6267..7775)
                     /locus_tag="IV247_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8777601.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(7741..8433)
                     /locus_tag="IV247_001527"
     CDS             complement(7741..8433)
                     /locus_tag="IV247_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP8777602.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(8455..9429)
                     /locus_tag="IV247_001528"
     CDS             complement(8455..9429)
                     /locus_tag="IV247_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8777603.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            9729..10601
                     /locus_tag="IV247_001529"
     CDS             9729..10601
                     /locus_tag="IV247_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8777604.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(11059..13257)
                     /locus_tag="IV247_001530"
     CDS             complement(11059..13257)
                     /locus_tag="IV247_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8777605.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            13489..14481
                     /locus_tag="IV247_001531"
     CDS             13489..14481
                     /locus_tag="IV247_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8777606.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            14541..15962
                     /locus_tag="IV247_001532"
     CDS             14541..15962
                     /locus_tag="IV247_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAP8777607.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            15955..17454
                     /locus_tag="IV247_001533"
     CDS             15955..17454
                     /locus_tag="IV247_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAP8777608.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            17493..18887
                     /gene="uxaC"
                     /locus_tag="IV247_001534"
     CDS             17493..18887
                     /gene="uxaC"
                     /locus_tag="IV247_001534"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP8777609.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            19046..19729
                     /locus_tag="IV247_001535"
     CDS             19046..19729
                     /locus_tag="IV247_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAP8777610.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            19731..20567
                     /locus_tag="IV247_001536"
     CDS             19731..20567
                     /locus_tag="IV247_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP8777611.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            20578..22332
                     /locus_tag="IV247_001537"
     CDS             20578..22332
                     /locus_tag="IV247_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8777612.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(22608..22949)
                     /locus_tag="IV247_001538"
     CDS             complement(22608..22949)
                     /locus_tag="IV247_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP8777613.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(23017..25188)
                     /locus_tag="IV247_001539"
     CDS             complement(23017..25188)
                     /locus_tag="IV247_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP8777614.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            25399..26256
                     /locus_tag="IV247_001540"
     CDS             25399..26256
                     /locus_tag="IV247_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP8777615.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(26327..>27111)
                     /locus_tag="IV247_001541"
     CDS             complement(26327..>27111)
                     /locus_tag="IV247_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8777616.1"
                     /translation="LSIPTTTPEETLDQVFEFFDRYELKGIGIGSFGPIGIDKTKDNY
                     GYVLATPKKGWAQFDFLGTIKERYNIPAAWTTDVNAAAYGELKQGAAQGKNSCIYLTV
                     GTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPDDHYEGTCPYHKDCLEGLAAGPSIEA
                     RTGIKGQDLPQDHPVWAIQAYYIAQALINYTLTLAPEKIILGGGVMNQDHLLQKIRQQ
                     FVELMGGYMETPQVSEYIVRWGLPNESGIIGSLLLAEHEHQK"
BASE COUNT         8041 a         5601 c         4877 g         8592 t
ORIGIN      
        1 tccatcatac ttgaaaaact caaaatcagc ataacttcct ctttttttat ttagatcatg
       61 cactgctatc ccgataaaat ttccagtaaa ccctcctgag agtatacaca tgtttttttg
      121 cggacctact ttcttccatt cttcagttga tacgtctttc aaatgaaact gtgctgtctc
      181 tttctttaca gtgactttca aaaaagtctt cttttctgtg agtggaatcg tccaatcttc
      241 tatttgtgct actccgtttt ctgattctat catacgtagg cattttccct tgtcctcatt
      301 ccaagtaaca taacaataaa tataattgga ttcatttaag tacagtacca gtccagccat
      361 ctgattaaaa ttattaggca tgtattcaaa tgctgtctcc gcatcaaaag taaaatccgt
      421 ttgccgaata gctaacaaat gatgttcgaa ggtagattga agagattcac cggaaatcag
      481 acgtaaaaaa cctggtctac attttgtatc acaccaatca gcatttggca aaatgcgtct
      541 ggcattccag ttcttcttta gctctccttc aaatgtatcc ataaactgat gatctttttc
      601 ttgttgtacc tcttcatttg tctcaatcat tatttcttcc atcggagttg tctggctatc
      661 tttaagtctt aaccaatcgt cggacgtcca atacacttct tgaatagcgg tttctcttcc
      721 caaaatagcc gcaccgttta atggtctggt acaaagatat gtcatgtacc atcttcctga
      781 cggggtttgt actaagctcc catgtcctgt acattgcaat gtagaagcag gtttgtcact
      841 agctgtcagc ataggaaaac caggatcaag ctcgtaagga ccagtaatat tctttgctcg
      901 gcaaactgta actgaatgtc ctcttcctgt accgccttct gccgtcatca aataataata
      961 cccgttacga aaatacaaat gtggtgcctc cgttttcgct aattctgtac ctgcaaatat
     1021 tttatagatg ggaccatcaa gcttcttctt ctctgcatca tattcttgta atacgatacc
     1081 gctggattta ttcggtgtgt ccagacgata atcccatagt tcattgacaa gccatttttt
     1141 tcccgtttca agatcatgga acaaagaagg atcaaagccg ttactattta aataaattgg
     1201 ttcggaccaa ggacctgtga tctcagatgc agtgatcaaa taattgtgga catctttaaa
     1261 cggacggcct gcacttttga catctgtgta aacaagataa aacaatccat cagcataact
     1321 gatctgtggc gcccatatac ttccatttgt aggatttcct tgcaaagata cttgagtggt
     1381 caaaatatcc gttgtatgtg accagttgac taaatttttc gactcatata ctcggattcc
     1441 tggaagccat tcaaaagagg agacaataat gtaataccgc tctttcactc ttaatatatt
     1501 aggatctgga ttgaatcctc ttaaaatagg atttttcact ttgatagtct gcatgattca
     1561 ggctcctttt ctcattctta ctcgatataa actgaatgcc ccagctgcga aataaaaaaa
     1621 tgcaaaagga aacgcaataa actgagtgcc aatcaacaat atgaatcctg ttataaaata
     1681 caaaccagtg gcagttgtta agtattttcc tttagcagtc aaaaacaatg ctactgctaa
     1741 aaaagcaagt gaaataaaca acacaacggc aaacagacca gtcatgtcct gtaataatcc
     1801 aaatgtattt tctaaagaca aagttgaatg atctacagca agcgactgat aacttttttt
     1861 aaactctgac gcagacatat tacttaaagt aactgctaac ccgccaaaga aaatcaaaga
     1921 tagactgcca ccgaccattc ctagcaatag ttcccatttt cttttcattt ctgaaccccc
     1981 tgcttttcta aatgtatcca ttcaggtctt ttgatcgatg tttcataatt gttattacgt
     2041 tcagagaatt tgacagtctg ttcatttttc ttgtcatccc aattatccca accagttggg
     2101 tgaacatgcc cacctatctt acacgcttca aataagacat ttgcatacgg acgccacggt
     2161 cttcctaaat agacgtttct agtatcaggt tctgcagtca aggtacagct ttcaaaacga
     2221 taccctgttt tttgattttt cggcgtagat gctgctgtga tgtatccagg atttttactg
     2281 gctaagctat ggatatgaca ctgatgaaaa acggcatctg ccccaccaaa tatgaaatcc
     2341 accgttcctt caatccagca atattcatag gtctgacgac aatactcagg attggcagaa
     2401 gccggtgtac aaaaaggagt accatctttt tgcaaatcag gcaacggacc cgtgcataag
     2461 gtatcctgat aaccagatag tcgacagttt tttaaagata cgcgatctcc tgaattgaat
     2521 aaagcaacag cctgtcctac cttttctcct ggacctgctg tattttcgat gtgaagattt
     2581 tctaatacga cattcgatcc ttctaagaaa aaagtagcac ttcggaaagt ccctaactgg
     2641 ctaccgtcaa gatactcctg ttttgcaaaa cgattaccgg agataactac tgttcctgct
     2701 cccacaagtt taaaattgga taatctgctt tctatatatt catcatatct tccttctttt
     2761 actatcaaga cctttggatg gctatccggc aggcctgcca aataatcgat tccttcttgg
     2821 atagtttcaa aatccgtcgg ggtatctata gcattctttc caattatcag tttgtctttc
     2881 atcctcataa atgctccctt atgaattgtt tccaaatatt tctcatttct tgtgtttcca
     2941 tatgaccgcc tgttaaagaa aaagaagaaa agttttctgg actacccatt gattgatact
     3001 ttttgtccag cccttttctc agcttcagta cgccttcgtc caagcacatc gtatcgtttt
     3061 ttccaactaa gctaagacga gggcgcggag cgattttttc ttggattgct aacgttgtat
     3121 aagctgtcaa tagatttggc acatagtaat aaaacccatg atgatctagg cggcgatgta
     3181 gcaaaagtat ttcgatgtcc acctgtgccg ctagatcaat acaaactttg acacgagaat
     3241 ccaatgctgt cacccaccag cttaataagc ctcccataga aagaccaatc gttgcgagcc
     3301 gttgagtatc tgtgtcttcc cttgtctcca aataatcgat caagctgatc acatcataaa
     3361 tacgcattcc ccataacgtt tttcctgtta ataaaaattg tttgaacaac tcgctttcag
     3421 aaatacctcc gcgttcttca aatccccaag catcaatcga ccaaacagca tatcccattc
     3481 ctgtcagtac ttctgcaaaa gatggagaag ctaagtaagg agcaccatgg atcaattcac
     3541 ttttcccgac tgaaaaatct ccgccgtgag aatgcaggta aaggaccggc ggaaacggtc
     3601 cttctttttc tggataagca tagagggctg gtactgtttc caattcattt aagtacaatt
     3661 gatagtattc aagcataaaa ccgttttgtt tttctcggta taatcgtttt ccactagggg
     3721 tatgtctgct agatggagca cctaaaaaat cattcaactg cataagcaaa gtttttacga
     3781 acaaccagtt catccatatt ttctgattga tttgattgga atttctcaat actgatttct
     3841 ttagtatttt ctaatagaaa tgctggtccc tcgtgatttt caatcgacat ctggttaaag
     3901 gaaaccttac ccgcaaagcc aatataaaat ccttgtctgc tcatttcctc tattcctgtc
     3961 atcatagcag gaacaccggg aactgcatct tgggccatcg agacatcgat attgtcaaat
     4021 gtgatttctt gtatgaattg ttccgctaat ccataaataa aacctgcaga agcatggaca
     4081 ttccttgccg tgatattcga aaaatggatc cttctgaatg ctggtgtccg ttcatctatc
     4141 ggataagcct ttttttccca tacatacggt tctttccctc gcggaccaca gaaataataa
     4201 agattcagta taaatggaca catcacatta tccatgacta tattggatac tcgaatatct
     4261 tcaacgatcc cgcctcgacc tcgtctagat ttcaatcgta ttcctcgatc agtttcttgg
     4321 aagatacaat ttgaaatggt aatattacga atactaccac tcatttcact tcctaatacg
     4381 acaccgccat gtccatggac catcgtgcaa tttgtgatcg tgatattttc gcaagcgatc
     4441 ctttcgtacg tatcttcagt ccctgccttg attgcaatac agtcatctcc cacgtcaata
     4501 tggcaattac taatacgcac atttttgcat gattctggat caattccgtc tgtatttggt
     4561 gaatccgctg ggttcaagat cgtcaagtta tcaaacgttg cattactgca aagaatcggg
     4621 ttgatcgtcc aacttggtga ctggatcaat ttaatatctt tcacagtaat tcgatgacag
     4681 ttatggaaac tcatcaattt aggacgagga taagcgagat tatctggttc atttctaaaa
     4741 gtatgccacc atttttttcc atttccatct aatgtaccga aaccagtgac agagatgttc
     4801 tctacatttt gagcatagat acaggaagca taaacttttc gatggactcc ttcccaacgt
     4861 gaatgaacaa caggataatc ttttggatcg tcagagaatt tcagaaccgc tcctgcgctt
     4921 aaatgaagtt cgacattgct ttttaaaaat aaagctcctg ttagaaattc acctgctgga
     4981 acaactacgg tcccgcctcc gtcacttgct gcagcatcaa tggcttgttg tattgcttca
     5041 gtattcaatt catcaattga tgcgccaaat gtcaaaatat catacatatt tataatgcct
     5101 ccacttcaat caatgcttgc aagaatgcac caacgccttt ttgatcatta caaataattg
     5161 gttcactgat gtagtaagca tatgaaccat ctcgctgatc tgctccgcct aaccctgcta
     5221 cttgacagtt tttattcaag ttcagccagc cttcttttgt ttccagaaca aattcatcaa
     5281 gtaatccttg atgtgatttc ttcacttgtg gaagccattc ttctttgttc agcagtccca
     5341 aacgtattcc tttagccatt gcgtaaacaa acatactact gccggaagct tccagataat
     5401 ttcctttccg ttttgtttct gttgttactt gataccatgt cccgctgttt tcttcttgat
     5461 atggaagtaa tgcagccagt gtcttttcta gaatttcttt cagcaaagaa caatctgttt
     5521 cttcatgaag taattcatag gtatctgctg cagccatcaa ataccagcca atcgatcggc
     5581 tccaaaaatg atgggataga cctgtttttg gatcagccca tggttggacc ttcttttcat
     5641 cccatgcatg aatcagcaaa tttgtttttg gatcgaatag atgtttataa ctgatttcaa
     5701 actgtctcaa aacatcttgg taatcttttt cttcactaaa attctggata aattcagcat
     5761 aaaaaggaga acccatatat aagccatcca accaaatttg ataaggataa atttctttat
     5821 gccaaaaagc tccttcggat gtgcgtggat ggtttgccaa ctgctgggaa aggagttctg
     5881 ctgctttttt atatttttct tcgcctgttt ctttgtacaa taaaaagaaa agctttcctg
     5941 tatttacatg gtcgatattg tattcttcaa cgctgtagcc acggatcgtt ccatcttctt
     6001 gcacgaaata gtcgatattg tcttttatga aatcaaaata aattttttct tgggttcgtt
     6061 cccaaagtaa ttggaaccct ttaagtacga cgccatagtc ataagaccat tttcctttat
     6121 accacttttc ttcatataag cgaggtgttc tttccatgat actttttgct aatttctttg
     6181 accagttttc cattatttgc tctcctttaa aaattaaaat aggatttggg acaaatacca
     6241 agaagtattt gtcccaatcg tctatattac tgtgccgctg ctttgtatgc ttcctcgtac
     6301 tcttgtgtga ttcgatcgcc tccttgtgct ttccagtctt ctacagcttt tttgaatcca
     6361 tcttcgtcaa gctgtcccat gatgtattgc accgtagcat caatgacgat tttttcaatt
     6421 tctgatccta cttcattgtt cgtcggtgaa tccaatggca cagaaggatc cattacagca
     6481 aattcttcat tttctttgat caattgatta gagaattctt tttcagggtc tgtatctttg
     6541 aaagagaacg tcacttcact aggacgagag cttgataacg gttgcacctc ttgttgccaa
     6601 agatccatat cgataaattc gatagaacca tcatcattca attcataatg cgttccttcg
     6661 ataccgccag tcatcaattt atacacatct tcttctaaaa gatcatcaac aaattgaagt
     6721 aatttcttca attgtgcttc gtcttttact tgagatttgg gaaatgctag caagccgcca
     6781 ataccatttc cttctgacca aacatgataa ccgccgcctt caccatctga gatcttgttt
     6841 acaggcacta attccatatc atcttgaata ccttgaccca tcgtcttcag attcttgata
     6901 tccaccattc cagtgtagat tccagttctt ccttgtgcaa attgttcttg ctgatcagtc
     6961 ttttgggtaa ctgcaaagtc ttgtgccaaa tagccattct caaatagatc tcttgaaaaa
     7021 ttcaaagctt tcatatactc ttctgtatca aattctggag tgaatttgcc attatcgtct
     7081 acagcccagc catttggtgc tccaaaataa gaagtgaaaa gtttaaagct ggtataacga
     7141 atatcactag gcgcacgatc gccaaaacca actgtgtcgt ccacaccatt tccatcagga
     7201 tcgttttctg taaattgttt agctacttca taaagctcat ccatggtagt tggaacatct
     7261 agtcctagat tatccaacca gtcttttcga atgactaagc cagcacgagc gtagtctttt
     7321 tgtatcggta cgccgtacaa ggcaccgtcg atactcgcag cttcgattct atcttcagaa
     7381 atttgactta aatttttata gtcttttaaa tattctgata catcccagaa tacgcctgat
     7441 tttaacgcat tgcggacaga agagtttttc aacatcgcaa tttgaagtgt aacgatatct
     7501 cctagttcat cagaagccaa tgcagttgtt aatctttctt cttttgaagc atcaggaatc
     7561 cagttgaatg tcagttccgt atcggttttt tcttcgatca agttcacaat cgtgtctgta
     7621 ggcggtgaag catgatgcaa aatatttaac caagtgattt tagcttttcc atcttctgtc
     7681 tctgaagaac tgttacttga actgctgccg catgcggcaa gtgctagaat tcctgcaaaa
     7741 ctaagaccta attttacgag cgttgctttt ttcataaatt ttctccttca ctgttttaat
     7801 aatatttttg ttttcttctg ttttttctgt atatgagttc ttccattcca aatggcaaaa
     7861 tatagaattt gctgtccacg tgactgcgga aaagaagaga ctgccaccga taaaggttaa
     7921 aacacctggc attgtttgga tgatcatcag ataaatccct accatactta atatcaacac
     7981 taatgagtaa tgaagatgtg agaatccgaa taaaaatgcc attttcattt tgaaaaaata
     8041 tcctttccaa tcataatgag ccatgattgg gaaaatatag agtgctgaga gcacatatat
     8101 gaacgcagca atatacaaag cgatcctaag ataccaattg tgtatgaaag aaagatccac
     8161 aagtaaaaca atcccgatgg cgccatatat ccagctcatc agcaaggttt cacgatagtt
     8221 ttctttaaaa tatgtgaagt acgttttcgt caaaggaaac gtttcttttg agcgcagcca
     8281 ttttctaatc actgcaacca tcgcatacga tgcaggaccg aaagtaaaaa ctcccaaacc
     8341 tgcgatgact aaaccgcccc acagaaaatt caaataaacc aactgtaaaa tacgatttag
     8401 ccttccgttt attttatcta atgactgcag catatctttc cctctctttc tatcttagta
     8461 aacgccgtct tctcctagct ttttggcaat cttattagct aaaattacca taaacaatcc
     8521 tacgacccct ttaaacaatc ccattgctgt actgaaactt aactgtccat tcttcaatcc
     8581 agctgtataa atataggtgt cgaaaatttc tgcaacgctt cgattcaatg aattcaataa
     8641 caagtacata tgctcgaacc ctaagtctaa tgtctgaccg atttttagaa tcagcaaggt
     8701 aatgattact ggacgaattg ccggcaatgt aacatgccat gttttgcgta agcgtcctgc
     8761 tccatccatt tctgcagctt catacaactg tgtatcgact gcagttatcg ctgaaaggta
     8821 gatgatcgta gaccagccca actctttcca gatcacttgt ccgatatata ctgtgcgtgt
     8881 ccattcaggg gatgttagaa aactgatttt ttcaaatccc attgttgcaa ggaattcatt
     8941 caatacccct ccgtcaacat tcaaaaatac ataagtaatc gaaacaatga ttacccaaga
     9001 catgaagtga gggatgtaaa tgatcgtttg aatcccgttt ttcaatcgtt tgttcgttac
     9061 ttcatttaat agtaaagaca aaatgattgg catcggaaaa aacacgaaga tatttaatcc
     9121 gaacaagatc aacgtatttc ttaataatgt aaagaatgtc ggttctgtaa acaaacgaat
     9181 gaaatgtttg aaaccgaccc aaggacttcc cattattcct aaaaatggtt gataatcttg
     9241 aaaagcaata acgagtcccc ccataggaag atacctaaaa atcaagaaat aaaggactcc
     9301 cggcagaatc atcacatata aaaatttatt cgttttgatt cctgcaatta ttttttttcg
     9361 tcttctgagc ttttcttctt ttttcgatag cgtcagttga aatggttctt gtttctttac
     9421 actttccata agcccctcct tagttttgtg aatgtttgct gcttatgact tcatgataga
     9481 taactatctg aattacgtat ataatcattt ttaccgtaag taaaaacgct tacatagcaa
     9541 tgatttttgt gctatacaaa acgataaagt gtattttttc acaaataaac tacgcatagc
     9601 aaaccttcta tttttttcta tctgacactt agcataggaa atgattatat acgtaatctt
     9661 tcaccgatga taaattgaac atatagaaac agaaacattt aatttatttt gaggaggagc
     9721 ctatctatat gaaaaagaaa gtctctatag gagaaagaag ttttacactt ttcaacaata
     9781 cgtttctaat tttgctagct ttgatctgta tcgttccttt tttaaatatc atcgctacat
     9841 cttttgcttc tacacaagaa gtcgtcgcga aaaagtttat cctttttcct actacctttt
     9901 ctttagacgc gtatcgctac attctttcta cgccgaccat ttttagagca cttgctgttt
     9961 cgattggtgt tacaggtgtc ggaacgatcg tcagtatgtg tgcaacttca ctgatggcct
    10021 acggtctttc cagaaagtat ttgtttggtc gaggtttcgt caactttctt gttgtatttt
    10081 ctatgttgtt cagcgggggg atgattccaa cgttcttagt tgttcgttct ctagggctgg
    10141 tcaattctta ctgggccatg atcctgcctg tggctgttaa tgcaatgaat atgatcatta
    10201 tgagaaactt tttccaagcc ttgccggata gtttggaaga atcagcaaaa atggatggtt
    10261 gcacagattt tggtgtattt ttcaaaatta tgctgccatt agcgttaccg tctattgcta
    10321 ctatctcgct tttttatgct gttacttact ggaacactta tatgactgct attttgtata
    10381 tcaacgattc atctaaatgg ccgatacaga ttcttttacg ccaaatcgtc attgtatcaa
    10441 gtgggatgca agcagaaagt agtgccgttg atgtgattcc accagcacaa acagttaaaa
    10501 tggctgttat cgttattgca acagtgccta tgctgattgt ttatcctttt gttcagaaat
    10561 attttgtcaa aggagcactc gtaggttctg tcaaagggtg attatgtact gaacgcaaaa
    10621 tggtagttgt gtcagaagtg gtcagcttca agaaataaga aggcatttac gaaaattgct
    10681 tcttaaattt ttgtgaaatt tcggcttatt tttcgaagaa tctactcctg gaacaccgtg
    10741 tatcagaggt tgtgaaagag acgttcagcc ttgagcgatg ataaaaactc tcacaaacat
    10801 tgcgacgagt aacccacagg agcaataaga agcgattttg aaaaatagct tcccatattt
    10861 cacaaaatta tgccttattg tcgatagcct gtctcttgaa cgccatttat tcgtaaaaaa
    10921 gaactgtgac aagacttttg tcacagttct ttttgcgtgc ttagccttga aatttaacta
    10981 tccataaata caaatactag ggttattcta acttctttta ttgcttgaat cagtccacaa
    11041 gaaaaacagc ctcttgattc acaaatattt ttttcgatat tctcccggcg taatcgattc
    11101 cttcttttta aagaaacgaa taaaattttg cggatttcga tattgcaatc tttctgcaat
    11161 ctcttttacg gagaggttag tctctttcaa ccattttttt gctacctcta aacgatgatt
    11221 ttgaacaaaa tcaccaaatt tttctcctgt ttccttttta aatacattac ttaaatagtt
    11281 agggttataa tgcaacctat cagcgatcac atctaaagac agatcacgat catattctgt
    11341 gtagacgata tgcataatat tctccgacaa atttttaaat tcatgatcag tgattgcttg
    11401 agagcattct gtgattggca gaactaattg ttctaaaaca agtttttcta gttctttggg
    11461 atgataattg ttcagtgctt ttacataaat ttgtttcatg ttatcaaagt tttttgaatc
    11521 tactcccaat aactgtccta attgaacgag ttcattgatc aagcggaccg aaactacttc
    11581 ccgactcaaa ggatttttat tttgtgcaaa caatgcatca atcaattcat gtactaaatt
    11641 cttcacttcc tgatttccag aacgaatggc ttcaaacaat cgattttgtt cttctatcgg
    11701 atatctaata agtgtttttt catgagcagc aggaacgatt tcctgataaa aaataattga
    11761 acttggacca gtatttacac gataatacag ggcttctttt gcccgatcaa ccgattgctt
    11821 gctttcattc agcgtttcga accggttact gatgcctata ctgatagtta aattcaagta
    11881 ttcttttaca cgtttttgaa tgagtcggca atactccatg acttttttgc ttgcttctgc
    11941 atcatcttta tccattcgat aaatagtggc ttgcatttct tcattcaaaa cgatcggcag
    12001 cattcgattt tcctttggca caatttccgt gatcatgttg ttgatcgcta aaagtagcaa
    12061 atcacgctcc ccaccgtgac tatcttctaa cagatcgatt tgaaccaaag ctgtataata
    12121 gcagtcattt tgagaactat aaccaaattg ctgcaaccgc tgatctagtt ctcgcctttc
    12181 tactcgattt ctaaataaac taagtacaaa tagcgtttcc agctgtggct tttgggtaac
    12241 taagtgtgca cttaacgctt ctttttctcc tatgatccga ctgactgatt gagcgaccaa
    12301 atctagttct ttcccactga atccttttga tttaagattc agacgttcct gtatttgaga
    12361 aatcggacgg gcaaatctgt ccgaaaataa gtaggaaaga agaccaatga gtcctaccag
    12421 tgttaaagac actgcaacta gcccgattct cagattgcgg atcattgcgc caattgcact
    12481 agagtcaact tctaatctgt aaatccaatt attataatct gatttcagta ctatatacgt
    12541 tttatctttt tctcttatca ctttcagctg attgtaatct agatccacgt ccgttaatac
    12601 agttggttgc tctttatttt ttactgtctg agtagtaaaa aggttgccaa cttcgttttc
    12661 aatagaaagt atgtcttctc cactttcacc aacaacttgt tctattgtat gtttctctat
    12721 cgtagcaatt cccaatgcat atttttcttt ttcaaacatt ggcaaagaaa tcaccatttc
    12781 gattcctttg tttttctttt gccaaaataa attatttttg ttatttatgt acttctgttg
    12841 atacttttca atttcctctt cggtcagttg actcaacgat ccattgatta ctccccattt
    12901 accagccaaa ctgactaaat cataattatt atcatccata acaatcgttt ctataaaatt
    12961 tagttctttc ctgatttcag aataagtgga gtaatctgta tatgtcagcg gatgcttgaa
    13021 tatgtccgag aaatttttgg tcgaaccata cgtagaaaac gaatattcaa tcgttctaat
    13081 tttccattca acgttagttt taacttgcct gagcaaattc tttttgtctg ttaaataact
    13141 cgattcgatc gattcatagg tattctggta aatgaataaa ctaaatccta tgacaagtac
    13201 ggctcctaat aaaaacatcg gtaaaaatat tttataaaat actgtacttt tcttcatttt
    13261 cttcctctcc tccttttaaa ataaaaggct ttcatttttc tgtaaaattg atactcatct
    13321 aattaaatga gcattccccg ctttcaagaa tagcataatt aaaaataaaa atccaagtaa
    13381 tttttaacgt gtgcaatctt ttcatatcaa agatctattg acttatcgta gttatcgtaa
    13441 tatgatgaat gcgttaacaa aaagtgaaaa agtaaggaaa atgatggaat ggtaacgatc
    13501 aaagatgtcg ctaaagcagc tggcgtttct catacgacag tttctcgtgc tttgaataac
    13561 agcagtttaa taaaaaatga aacaaaacaa aaaatacgac aactagctaa ggaaatgaat
    13621 tattcgccaa actatagtgc aaaaggttta gtgaatcaaa aaaaatatct aatcggacta
    13681 tttttctcaa gtttgcataa aggaacttca tcaaatttcc tagcagattc cattgcaggc
    13741 atccattcag tattagacac aaattacagt ttatctgttg aaagtatcga tgttgttgat
    13801 ttaacagaaa tcaacctgca acgctacgat ggactgatcg ttatgagtca aagtgatgat
    13861 gatcagcctt tcatcgacta tctcaaagaa aaagaatttc ctttcgttgt tgtcaatcgt
    13921 cacatcaatg atccagcggt tatcaacgtc actgccgatg atgcggccgg tgttatgcag
    13981 gcagtagatt acggtattgg atgcggacat acgaagatcg cttatattgg cggaaagaaa
    14041 aattttcgtt cgacaaaaga acgggaaaaa ggggtattga aaagtctaaa aaaagcgagc
    14101 atccctgtca atccagccta ttttttaaca ggagattaca gcttagaaag cggcctccaa
    14161 aacatggaat acctattatc actttctgac ctgcctactc tggttttttg cggcaacgac
    14221 gacatagcga ttggtgcatt gagagctgta catttagctg gcctccgtgt tccccagaac
    14281 atttctctga tcgggttcga tgattctcca gtcgtctcct atctcaatcc gccgcttacc
    14341 accgtacgta agcctttgaa agaaatgtgt caagaaggaa caaagctttt actagatttg
    14401 atcgaaggca ggacgatcga acaatctaaa attgagctct ccaccttgtt gatccttcgt
    14461 gagtcgatca attacttgta aactacaagt aattttatat aaaaatgtta acgtgtgcat
    14521 aacacagaaa ggaagtttat atgaaaaaac tgacaaaaga attagtgaaa aaaaacgaag
    14581 cacctgtaaa agttcttcag tttggtgaag gcaattttat gcgtggattt attgattggc
    14641 agattcagca gctgaataac caacaattgt ttaaaggtaa tgttgcaatc attcaacctt
    14701 tagaacaagg tcttggaaat atgatgaacc agcaagataa tttatatacg gtcattttac
    14761 aaggactcag caatggcgaa atcgttgata cttccgagat tatcaccgta gtagaacaaa
    14821 tgatcaatcc ttatgatgaa tgggatgctt atctatctct agcagaaaat gacgacttgg
    14881 cctatatcgt ctccaataca acagaggcag gaatccaatt cgtggagact gataatttaa
    14941 cagatactcc cccacagagc ctcccgggca aactgacagc ctttttatac cgacgtttca
    15001 aattaaacaa agctggcttt accatcattc cttgcgaact gattgacaga aacggcgaac
    15061 gtctaaaaga aatcatttta cgctacgcag aaaaatggga tttagaacct gcttttgttg
    15121 agtggttaga aaaagaaaat gttttctgtt gtagtttagt tgatcgcatc gtcccaggtt
    15181 atcctcgcga agatgcaggt gttttcaatc aacggcataa ctatgaagat caattgatgg
    15241 tcaaagcaga accattcatg ctttgggtga tcgaagggcc taaagaattg gagaatcaac
    15301 taccattcaa acaggctggc ttgaatgtca tcatcacaga tgatatgact ccttatcgtg
    15361 aacgtaaggt gcatttgctt aatggaccgc atacagcgat ggtccctctt gctttgttgg
    15421 cggaattgga aactgtagaa gaagtaatga aagatccttt attctccgca tatgttgatc
    15481 agttattcaa tctagaattg attcctatgc tttctttacc taaagatgaa ttagccattt
    15541 atgctgacca aatcaaagaa cgtttcttga atccttttgc ccatcacaaa ttagaagcga
    15601 tttcattaaa tagtgtttca aagttcagca cccgtctgct tccagtgttc aagaagtata
    15661 tcgaagaaca aaatcaggta ccaccgttaa tcactgtttc actagctgcg ctacttctta
    15721 tgtatcgagg cgatcaagta aagccacatg atgatgaaaa gacgataagc gaatttactg
    15781 atgcttggtc tgataatgga acagctattc ctcgattatt gcaaaacgct gcgctttggg
    15841 gagaagattt gtctcaaata ccgaatgtga cagatacagt tcaagaaata gctgcaaaga
    15901 tcgatcaaaa aggtgtccgg tcagtattac aagaaatgaa aggaacgatg gtccatgctt
    15961 gaaaccctgc aaccaacaat gaaattaaat cgacgagaca gtgtggtcgt cgcattaacc
    16021 cctattccaa aacaaacaca actgacgatt gatcagcaaa cgatcaccac tcttgaagat
    16081 atcccgcaag gtcataagat tgcattgggt gatttgaaag ctggagacaa cgtgatcaaa
    16141 tatggctatc caatcggtca tgtgacgaca gacgtaactg ccggtcaatg gctgcacact
    16201 cataatgtaa aaaccaatct ttctggtgaa ttggattatc gatatgaaaa agatgtccat
    16261 ccaagtcatt acctttttga gaaccgaaca tttcaaggtt acttgcgaaa aaatggaaaa
    16321 gtagggattc gtaatgattt gtttttagta cctaccgttg ggtgtgtgaa tggcattgct
    16381 gaactgatcg tcaaacagtt caaggaaaaa catccagatc tgggacagtt tgaccatatt
    16441 acgattttaa agcatcctta tggctgttcg cagcttggaa aagatcatca aaatactcga
    16501 gaaattttag cggacgcagt aaatcatccc aatgccggag gagttttagt atttggatta
    16561 ggttgtgaga acaatacagt tcctgaattc aaaaaaattt taggtgaata tgatgaagaa
    16621 cgagtgaaat ttctagtggc tcaagatgtt tacgatgaga tcgaacaagg tgtggctcta
    16681 ttagaagagc ttttaacagc ggcggaaaat gaccagcgca tatcagtccc gctctctaag
    16741 ttgaatgtcg gtttgaaatg cggcggttct gatggtctat cgggaataac tgctaatcct
    16801 ttacttggtg cattttctga ctacttgatt gctcaaggag gcagcacgat cttaacagaa
    16861 gtgccagaaa tgtttggcgc agaacaagta ttgatggcac gagcggaaaa tgaagaagtt
    16921 tttgactcaa tcgttgactt gatcaatgac ttcaaacagt atttcttgtc ttacggggaa
    16981 ccagtttacg aaaacccatc tcctggtaat aaagcaggtg ggatcactac attagaagat
    17041 aagtctttag gctgcacgca aaaatcagga agctctcctg tcgttgatgt gctacaatat
    17101 ggcgaaaaaa tcagaaaacc agggcttagt ttattgcaag ctcccgggaa tgatcttgta
    17161 gctgcctctg ctcttgcttc atccgattgc cagttggtct tgtttaccac aggaagaggg
    17221 actccgtttg gctcatatgt cccaacggtc aaggtatcta cgaatacaac tctttttgaa
    17281 cgcaaaggtc attggatgga ttttaatgca ggcgtcttat tagaacaacc aatggaagta
    17341 atcttagaat cgtttgttca aaaaatcatc gctgtagcta gtggagaaga aacgaaaaat
    17401 gaacaaaatg acgttcgaga aattgccatt tttaaaaacg gagtaacact ttgattttca
    17461 taaaacagta taaaaagaaa gaaggaagat ttatgttttt atctgatgat tttttattaa
    17521 aagacgattg ggcgaaaaag ctttaccatt cctacgcaaa aaacatgccc attattgatt
    17581 atcactgtca cttagatcca aaagaaattt acgaaaacaa caatttcagc aatctgacag
    17641 aagcttggtt aggaggcgat cattacaaat ggcgcttgat gcgagcttgc ggcgtgcctg
    17701 aagaaaaaat cacaggaaat gcttctgatt ttgaaaaatt tttagcttgg tgtcaaaccg
    17761 taccaaaaat tatcggaaat ccgctttaca gttggtcaca tcttgaattg aagcgtttct
    17821 tcggtattga cttgttgata aatgaagaaa atgcagagaa aatttggaca caagcaaatg
    17881 aaattttggc agctcctgat tttcgccgtc gggaaatcgc aaaaaaatcg aatgtagaag
    17941 tgatttgtac aacagatgat cccgcagacg acttgcacta tcatcagttg cttgctcatg
    18001 aagaaccaga tttgaaggtt ctaccttcat tccgtccaga taaagcactc aatattgaaa
    18061 aagaaggatt taatgcatgg gtcacactac tggaagaagc agctgaccaa acgatcacca
    18121 cctatcaaga actgatagaa gtcttagggc agcggataga atacttccat caacatggat
    18181 gtcgtgtttc cgaccacgct ttagacatct tacgctataa agaagcggat gaaacagtat
    18241 tagaagagat ttttcaaaaa gcacgagcta acaaactatt atcttcagat gaaatcgatg
    18301 cttatcgtac agaaacactg atacatttga cccacttcta tcattctcat aattggacga
    18361 tgcagctgca catccatgct tatcgtaact gtaatacaca aatgttggaa aaattagggc
    18421 ccgataccgg ctatgatgga atgaatgatc tttctttgac tattcctctg caaaaattat
    18481 tgaaccgtgc agaagaaacg gatcagttgc caaagacgat cctttattca ttgaatccga
    18541 atgactatcc tgcgatttta gctttgatgg gctgtttcca aaaagaacag aatggaaaac
    18601 tgcagttagg ttctggatgg tggtacaacg atactcgagc tggtatgcgc gatcaattga
    18661 cacaatttgc tgatggtagt gctttgggga attttgtcgg catgctgaca gattcaagaa
    18721 gtttcctgtc ctacactaga catgaatatt tccgtcgtgt tctttgtgaa tttttaggcg
    18781 aaatggtcat gcgaggagaa gcacctgaag atgaacaact tttaggaagt ctagttcaag
    18841 cgattagtta tacaaatgct aaaaattact ttggcttttt tgcctaattg attaatagta
    18901 acagtaacga atcaaaaaca ggatagtgaa ataacggaat ccgtttatcc acgttttaga
    18961 gaagaagacg gaccaaattt gctccgtctt cttctcttgc tgttctgctt cattgcctat
    19021 aaacctttat gaaagaagag ataacatgcc tactatccat attgccggtg attctactgc
    19081 ggcagaaaaa gatgaaaaca agcgtcccga gacaggttgg ggagaaaaaa tctctgctta
    19141 tttttcaccc gagatccaga tcaataacca agcaaagaat ggacgcagca ccaaatcatt
    19201 tatccaggaa ggaaggttag cccgtctagc ttcacagttt cagccgaatg atttcttatt
    19261 tgtacaattt ggacataacg accagaaaat ccaagaagaa agaggtacgc ttcccttcgg
    19321 tagctattta aaaaatctgc agatattttt tgaatgcgca aaaaaagcga acgtccaaat
    19381 ggtcttactg acatctgtga cgcgacgaga ttacttagaa aacggaacat tgaatccaga
    19441 tatacttgga gactatccaa aagcgatgcg tgcatttgct gagaaacacc atattcctct
    19501 tttagatgtg ttctcaagat cacaagaatt gttccagacc ttctcaaaag aagagacaaa
    19561 aaaattctac ctgcatctta tacctgatac ttgtaaaaat tacccagaag gactaaaaga
    19621 caatacccac tttagtccag aaggcgctga taaagttgcc cgaataattg ttgaattgat
    19681 aaaagaatcc cgtctgcctc ttgctaatta tttacaaaaa ggagtttgag atgtttccat
    19741 taaatttagg catccgtgcc cacgatttag atgttcgaaa tcgtgaagaa atcgttcaaa
    19801 aagtatcaca gcttcacttg tcccatatcc aatttgcacc acaaaaagca tttcctgaac
    19861 aacttaaatt aactgacatg acacttggga gtgctgccta ttttggtgaa tacttccaaa
    19921 aaaacaatat cgaactttcc attttaggat gctatatcaa cctatccagc aaaaatcaag
    19981 aagttcgtgc acaagcattg gcaactttca aacagcaaat tgctctgtgc tctgcctatc
    20041 aagccaagat gattgctacg gaaaccggta gtgtctctgt cggttacaca aaagaaaatt
    20101 ttactgaaga agcttatcag atcgccagaa attctatcat tgaattagtc gctcatgcag
    20161 aaaattttgg cgtaactgtt gctgttgaag cagggatcaa tcatccaatc tataactatc
    20221 agttagctaa acgtttaatc gatgaagtag cttcaccaaa tttaaaaatc attttagatt
    20281 gtgcgaattt gatgcataaa gaaaatcatg ctgatcagga aaaagtgatc cgaggtgcct
    20341 tggacaattt acatggttat attacagcgc ttcatctcaa agactatatc atggaaaatg
    20401 gaaagatccg catggtacct gttggcaagg gctgtttaga tttccgcccg gtactgcatt
    20461 atatcaaata tgagcgtcct ctaatgtacg ccactcttga agctactcct gagatttatg
    20521 ttcctgaagc aatagaacac ttgcagttta tttaccaaga ttgttaagga gaagattatg
    20581 gaggaacgaa tacagaaaag ttatcaaaaa tatcatgcaa atgcttttca gacattgctg
    20641 catctttatc agggaaatta ttggcgtttt gccatgtcag gactcttttt tattatcaaa
    20701 catcttccat cttggttaat gccgatcgct attgccaatg taatcaatgc agcttcatcg
    20761 aaagattctt ctcagttgca ggccatttat ttcaacgctt ttttgatggc ttgtctgatt
    20821 ggtcaaaata tattgacgaa ttattttcat gtaaaatacc atagcacttc gatccgtcaa
    20881 gtagaagctg gactaagagc gacccttatc aagaaaatcc aagaactatc tattccttat
    20941 caaaaagaat tgcaatctgg acgaatccag tcaaagatca tccgtgatgt agaagctatc
    21001 caaacgttgt cctctcaagt atttgtcagc ggattaaata tcgctgtcaa tttattcgta
    21061 gctttaggta tcacgcttta taaaagtcct gttgttttca tgtttttctt attgactgtt
    21121 cctctcgccg cattcgttac tctctttttc cgtaaaagga tcagacagtc caactcagac
    21181 ttcaggcaag aaatggaaga gacttctgca aaagtgatcg aagtgatcga attagcaccg
    21241 attgctcgag ctcatgggtt agaaaaacac gaaacaactc gcttaaataa gcattttact
    21301 caaatctatg aaaaaggatt ccacttagat atcctgcaat ctgttttcgg atcagtcagc
    21361 tgggctagtt ttcagatatt tcagctgatt tgtttgattt tcactgggat attagcttta
    21421 caagggcgca tccaacccgg cgatgtcgtg atgtatcaga cgtatttcac gacgatcgtc
    21481 aattccgttt ctggaatgat cacgctgatt cctacgattt caaaaggcat ggaatctatc
    21541 acttctattg gcgaactgtt gattgctggc gatgtagaaa cttatcacgg taagaaaaaa
    21601 atccaacatt tggatggaaa ttttcgtttt gaggatgttt cttttcttta tccggactca
    21661 ctcaaaccga ttatcgatca tttttctcta gaggtgaaag ctggagaaac aattgctttt
    21721 gttgggccat ctggctctgg aaaatctaca ctccttaact tattgattgg atttatccag
    21781 ccgacttcag gaaaaatttt tctagatgat cagcctttgg atgaactgga tatgcgaagt
    21841 gtgcgaaatt atttagccgt tgttcctcaa accactttgt tgttctccgc ttcgatcaaa
    21901 gagaacatca cttatggctt gaaaaacgta tcaaaagaac gtttagatga agtcatcgaa
    21961 gctgcgcaac tttcttcact gattgaccag cttcctgatg gtctggatac cttagtaggc
    22021 gaacatggca acaagctgtc tggtggccaa aagcaacgta tctccattgc acgagcgttg
    22081 atacgtcagc caaaaatcat tttgctagat gaagcaacct cagctttaga taaccagtca
    22141 gagaaaaaaa tccagcaagc cctgaattac ctgacagaaa cacccactac ttttattgtg
    22201 gctcatcggc tttcaacgat caaagaggca gataaaatcg ttgtgatcga tgaaggaaaa
    22261 atcgttgaaa taggtactta cgaaaaactg ttagctcaaa aaggctattt ctaccgcttg
    22321 aaaaatgaat gacaaaaaat aggagctatg acaagacttt tgtcgtagct cctatttctg
    22381 aataaacggt gttctcctgc caactcctcg gtcacaagtc acaatagtca tcaaacatga
    22441 gaagctgttt tttgaaattg ttccttgtaa ctcagagtta aacggtaggc tcacgacctc
    22501 ttggtaaatg gtgttcaaga gacagcctat cggcaataag aagcaatttt gtcatcgctc
    22561 aaggccgaac gtctctttca caccctctgt tgtttcataa aatagtttta ttcgctgtaa
    22621 aggtcgcgaa ctggtgtcat gatgatacgg ttcaagtcat tgatgattgt actgaagcct
    22681 tgctcttttt gcatcaagtc attgattact tcttctgatt ggattttcac agccatttct
    22741 tgtgctttca ttgcatcttc gtcgctgaac tcttgtccac tcatttgttt ttcttgtaat
    22801 tcgatttgca tcgcttggaa gtctttgaac agtttgtgtg cttcttcgtt tgcttgtact
    22861 ttctcgtacg cttctttcaa cgctttgaat tctggtaatt cacggatctc acgttcgatt
    22921 tgattcgcgc tatcgtagat gtttgacatg ttatgccctc ttttctttta gtagttactc
    22981 tctattgtaa catggggtta ttcgttttca aaggggctac tctccttgat atttccgcca
    23041 gaggtcttta gccgcttcac caattccctg aacgccttct ttcaccatat cgccgaagtt
    23101 tttcagccct tcttttgctt gttcaccata ttgattcaag ttatcttgcc aatttccaga
    23161 ctctccatta gaatttcctg tgtttccaac ttgatctgca gctaccactt gtcctcctgt
    23221 ggcataggca tctgctacac taaatcctgt ttttgaagta tacggaagga tgccttctgc
    23281 cgctgatttg aagactttcc cgacaacatt tccacttgtc ccttccaaat agtgcagctt
    23341 gctggattct tcgtaaccca tccatgtcga aatcactacg tctggcgtgt aaccgatcat
    23401 ccattggtca tttgcttttg tggcatcgaa attagtttct gtggttcctg ttttccctgc
    23461 tatcgtataa cctgcaggat tcgcagcagc cgctgttcca tttgaaaatg taccaagaag
    23521 catgctggtc atttcatcgg ctgtttcctt tgatatgacc tgttttggtt ttttgtttgt
    23581 gttatcgacg atcaccgctc cagtcgaatc aacgattttc gtgataagat gcggcgtata
    23641 catctgacca tcattggcaa atgcactgta agcacccgcc atgatcaatg gagatactcc
    23701 tttttccagt ccaccaaggg ctaagccata atacttatca gattttgcta aaggcaggcc
    23761 gaattcttct gctttatcat atcctttttg taaaccgatc tcatgcaaaa gccatacagt
    23821 aggcaaattc aaactttgtg caacagcttg atacatcggt acttctcctt ggtatgtccc
    23881 gtcaaaatta tgcgcatcgt aataactttg cggttcgtct ttcaagatac tggaaggctt
    23941 gtatccagct tccagtgctg gtgtatatac gcttaatggt ttgatcgttg atcctggtga
    24001 acgacgcatc tgtgtagcaa agctgaatcc tctaaagaca tgatctcctc ttcgtcctac
    24061 tagtgcttgt acaccacctg tatttggatc taaagctact gaagcagatt ggaccatggc
    24121 accatctgaa gcatttgctg ggaataatgc actgttttga taagtagctt ctaattgctt
    24181 ttgataatcc tgatctaaag ctgtatagat tttatagcct ttattcatga catcttcttc
    24241 atcaagacca tactcgttaa ctgcttcatc gatcactgca tcaaaataat aaggataacg
    24301 ataaccagaa tctgaattat cgtatgtgtc attaagatac gcgctgatat ctgtctggct
    24361 ttctgcttgt tcttgttctt ttgtgatttt tccgttttct tccattacac ttagtactgt
    24421 gtttttccga tcattcgcat tttttgggtg atcgatcgga ttataaatac caggaccttt
    24481 cagcattcct gccaaagtag cagcctcaga cagcgtaaca ttcgttgcat ctactccaaa
    24541 atactttctg gaagcgtctt ggacacccca taccccgttg ccaaaatagg cattgttaag
    24601 atacatggtc aagatttctt ctttgctgta cttcttttcg atctcgattg ccaaaaataa
    24661 ttccttcgct tttcgatcaa acgtttggtc taatgtcaga tacgcatttt tggcaagctg
    24721 ctgagtgatc gtacttcctc cgccccctga tgttccgcca gacaagacca ttcggactgc
    24781 tgcacgggca atccctttga tatcaaaccc gtgatgctcg taaaaattcc tatcttctgt
    24841 cgagattact gcatcttgga tatagggaga aatcgcgttt aattctacat atgttccttt
    24901 ttgggcgtac agtgtgccag cttcttcgtt tttcttatca taaatgactg tcgattggct
    24961 taatcctgat tttaacgttt cgacatttgc ttgttttgct aaaataaaga ggtagatact
    25021 tgtcactaag acacagatca aaccaatcag caaaatcaat ttgttcacat gatatttttt
    25081 ccagatgcgc tttcgccatc gatggaattg tccgagatat ggtctgatcc aacgccaaaa
    25141 actgatcaaa gcagctttta tcttttctaa aatgctttga aaatccattt gcttcttctc
    25201 ctgtctattt gattcaatcg tcttatttta ccatagtgaa agagtgtttt cttcctgctt
    25261 aagttgtagt tcaatatgtt tttttacgtt agtgcaagta gagtaacata agaatgaaca
    25321 gaaaaatgta agttttttct taaatatgtt ttaaaaatgg tacaattagt ctgcgattgc
    25381 actgaaagga gtacacttgt gaaaatcacg atcactttac ctatgaacca tcctgaaaca
    25441 acaattcgcg acttgcttga aaaggaatgg ttagttccta gaaaggttcg acactttttg
    25501 cgtatacgta aaaacgtttg gatcaatcaa caacctgctt tgttccatca tgaagtaaag
    25561 ggcaatgacc agatcacatt gttattcgaa gaaacagatt ataattatca agaagttcaa
    25621 ttaggagatg cgaagcacat ccgtgtttta tatgaagatg agcatttgat tatagtggat
    25681 aagcctgcag gaatgaagac tcatccaaat gaaccgacag aaaacagtac cttactcaat
    25741 catttagctg cttatctcga cactaaacag caacgacctt acgttgtcca ccgcttggac
    25801 aaagaaacaa gcggtgccgt tttatttgca aaaaatccat ttgtcttgcc gatacttgga
    25861 agaatgttgg aacaaaaaat gatctatcgg agatatcaag ccatcgtatg gggagaaatt
    25921 gccaaagata tgaccatcac caataaaatt ggacgagatc gccatgatcg gagaaaaagg
    25981 gtcgtagatg ataaaaatgg tcagagcgcc ttgacacatg tcactgtcaa caaagtactt
    26041 tctgaacaaa cacaagtcta ttgtgttttg gaaaccggac gtacacacca gatcagagtt
    26101 catttagctc ataagggaca tccgatcgtt ggagaccctc tctaccaaaa tagaacagct
    26161 gaacgactga tgctccatgc attagaactg cacatgatcc atccatttac tcaagaaaaa
    26221 atagtcggta aagctttacc tggattatgg gaatagaaat aaaaaagaac agagtcttat
    26281 cagcagataa tgataagact ctgttctttt tgtgtaattt gtctgcttac ttttgatgtt
    26341 cgtgttctgc taacagcaaa ctaccgataa tcccactttc attcggcagt ccccaacgaa
    26401 cgatatattc acttacttga ggtgtttcca tataaccacc catcaattca acaaattgct
    26461 gacggatttt ttgaaggaga tgatcctgat tcattacccc accgcctaaa ataatctttt
    26521 caggtgctaa cgtcaacgta tagttgatca gtgcttgagc aatataataa gcctgaatag
    26581 cccaaacagg atgatcttgc ggcagatctt gccctttgat ccctgtgcgt gcctcaatag
    26641 acggtcctgc tgctaatcct tctaaacaat ctttatgata aggacaagta ccttcataat
    26701 ggtcatctgg atggcgtttc acccaaatat gtcccatttc aggatgtgcg atccctgaaa
    26761 aaatttcttc attgatcacg acacctgcac cgattcctgt tcccacggta aggtaaatac
    26821 aactattttt tccttgtgct gctccttgct tcaattcccc atatgcagca gcattgacat
    26881 ccgttgtcca agcagcagga atattgtagc gttccttgat tgtgccaaga aaatcgaatt
    26941 gtgcccaccc ttttttggga gttgccaaaa cgtatccgta attatctttt gttttgtcga
    27001 taccaatcgg gccgaatgag ccaatcccga ttcccttcaa ttcataacga tcaaaaaatt
    27061 caaatacctg atccaatgtt tcttctggtg ttgtggtagg gatacttaat t
//
LOCUS       DACDIG010000035        26804 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.035, whole genome shotgun sequence.
ACCESSION   DACDIG010000035 DACDIG010000000
VERSION     DACDIG010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.035"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(12..212)
                     /locus_tag="IV247_001542"
     CDS             complement(12..212)
                     /locus_tag="IV247_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8777617.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(517..1749)
                     /locus_tag="IV247_001543"
     CDS             complement(517..1749)
                     /locus_tag="IV247_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP8777618.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            2003..2572
                     /locus_tag="IV247_001544"
     CDS             2003..2572
                     /locus_tag="IV247_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8777619.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2750..3190)
                     /locus_tag="IV247_001545"
     CDS             complement(2750..3190)
                     /locus_tag="IV247_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAP8777620.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            3348..4112
                     /gene="map"
                     /locus_tag="IV247_001546"
     CDS             3348..4112
                     /gene="map"
                     /locus_tag="IV247_001546"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAP8777621.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4144..5067
                     /locus_tag="IV247_001547"
     CDS             4144..5067
                     /locus_tag="IV247_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAP8777622.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            5143..6282
                     /locus_tag="IV247_001548"
     CDS             5143..6282
                     /locus_tag="IV247_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAP8777623.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            6275..7075
                     /locus_tag="IV247_001549"
     CDS             6275..7075
                     /locus_tag="IV247_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8777624.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            7075..7902
                     /locus_tag="IV247_001550"
     CDS             7075..7902
                     /locus_tag="IV247_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8777625.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7880..8614
                     /locus_tag="IV247_001551"
     CDS             7880..8614
                     /locus_tag="IV247_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8777626.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            8714..9580
                     /gene="rfbA"
                     /locus_tag="IV247_001552"
     CDS             8714..9580
                     /gene="rfbA"
                     /locus_tag="IV247_001552"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAP8777627.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9594..10166
                     /gene="rfbC"
                     /locus_tag="IV247_001553"
     CDS             9594..10166
                     /gene="rfbC"
                     /locus_tag="IV247_001553"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAP8777628.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            10188..11216
                     /gene="rfbB"
                     /locus_tag="IV247_001554"
     CDS             10188..11216
                     /gene="rfbB"
                     /locus_tag="IV247_001554"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAP8777629.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            11314..12165
                     /gene="rfbD"
                     /locus_tag="IV247_001555"
     CDS             11314..12165
                     /gene="rfbD"
                     /locus_tag="IV247_001555"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAP8777630.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            12200..14233
                     /locus_tag="IV247_001556"
     CDS             12200..14233
                     /locus_tag="IV247_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777631.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            14277..15557
                     /locus_tag="IV247_001557"
     CDS             14277..15557
                     /locus_tag="IV247_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAP8777632.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCRSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVTEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            15767..16573
                     /locus_tag="IV247_001558"
     CDS             15767..16573
                     /locus_tag="IV247_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8777633.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16585..17805
                     /locus_tag="IV247_001559"
     CDS             16585..17805
                     /locus_tag="IV247_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8777634.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGKVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            17795..19381
                     /locus_tag="IV247_001560"
     CDS             17795..19381
                     /locus_tag="IV247_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8777635.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLYINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAQPQNSNYVKVLEVIQQTKQDEIIQQIPFNNFTGENETLSFHVSELA
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKDLPEFFLTDIAG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHYLIENNTRLD
                     QTLKTTINRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSLCIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVLREEVNQAKQLAEGAKAGFELRILCEK
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19420..21558
                     /locus_tag="IV247_001561"
     CDS             19420..21558
                     /locus_tag="IV247_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8777636.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEVQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPAEHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVHPGRISGFYEITYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(21926..22909)
                     /locus_tag="IV247_001562"
     CDS             complement(21926..22909)
                     /locus_tag="IV247_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HAP8777637.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            23287..24678
                     /locus_tag="IV247_001563"
     CDS             23287..24678
                     /locus_tag="IV247_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP8777638.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            24694..25734
                     /locus_tag="IV247_001564"
     CDS             24694..25734
                     /locus_tag="IV247_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8777639.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            25753..>26804
                     /locus_tag="IV247_001565"
     CDS             25753..>26804
                     /locus_tag="IV247_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAP8777640.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTT"
BASE COUNT         8570 a         4479 c         5435 g         8320 t
ORIGIN      
        1 ggtacagggg gttattttgt ataagtagac attgtagttt ctaaactgct atgtcctaaa
       61 cgtttcgata cgctaagtat atttactcct tgataaagta aaatagatgc atgcgtatgt
      121 cttagtccat caataaacag tcgaagagaa aatagggaaa aattgattac taaagaaaaa
      181 cttactaggt cgacggtgta gttgattttc atacttcaat agattcgaat taaacgattt
      241 cgattggctt ataccagtcc tatccaacga aagatatatc gttcttaact ctagcggtat
      301 aacttgaagc tttacgtaaa gccaatggga atgaactgga gatgactttt tattattttt
      361 agtaatagct gatgggttaa tacttacaac tttccttttc aaagtaccat ataaaaaaac
      421 ggtggggttg tttagcgaaa taaaggatct aagccaatag cttagatcct ttattctatg
      481 ctttatatta ttttttgcat gtgtgcgaaa gtatactcaa gcccaatcgc catttcggaa
      541 aatcggtact gtagagccat cttcacggat tccgtcgata tccatcttgt cagatcctac
      601 catgaaatct acgtgggttt gacttctatt caatccagct tctgctaatt cttcatcgga
      661 catttctgta ccgcctttca cactgaaggc ataagcagaa cctagtgcta agtgattcga
      721 tgcgttttca tcaaataatg tattgaaaaa gatgatgcca gattgagaaa taggtgaagg
      781 gtcaggtaca agtgcgactt ctcctaaacg acgcgcacct tcatctgtat caagcagttt
      841 tgctaagaca tcttctcctt gttcggcaga aaaatcaaca acttttccat ctttaaaagt
      901 aaatttcata cccgagatga tggtgccagc ataactcagt ggctttgtac ttgaaatgta
      961 accatctact cgatgactgt caggtgctgt gaacacttct tccgtcggca tatttgccat
     1021 gaatttttct ccacgagcat tatagcttcc agcgccttcc caaagatggt ttttaggcaa
     1081 tccgataatg atatctgtac cgggagcagt atagtgaagg gcagaaaatt gttctcgatt
     1141 caattcttct gctttttttg ctaatttttc atcatgcttc ttccatgcta agacagggtc
     1201 ttcttcatag acacgagtcg ttttgaaaat ctgatcccat aatgcctcaa cttgtttttc
     1261 ttctggcagt tctgggaaga cttttgctgc ccattgttta ccagccgcag ccacgacagt
     1321 ccaactgact ttatttgctt gagttgcctt gcgtaaattc attagtgctt tgccattcgc
     1381 tgattgataa gacgctactc gatggctatc tacaccagca aatgcatcgg gatcggcaga
     1441 aacaacgctg atcctgcttg ctcctttttc aagccattca tccgcttgat cgattttaga
     1501 ttgtggtaca ttctcaatac gatccgttgc agcatgtaaa agaaattctc tttggatctg
     1561 gtcgtcagtc cattgaacga taacctcagc ggctcctagt ttataagctt cttgtgtgat
     1621 caatcgagca aggggtgcct gttccacgct gatctgtaaa acaactgtgt gtcctttttc
     1681 tgtggctacg ccagtttctg caattaagcg tgcatatttt tttagaagtt cattaaaatc
     1741 agataacata ataaaaattc ctcctcacca ttttttctgg taactattag catgatacca
     1801 tttaattatg attcaaacaa tgaggcttta aataaaaaaa ggaggttatt tttttaaggt
     1861 taagtttcat gatatatgtt actacttgct atttcagctt gtgaagaaaa tcaaaaacta
     1921 attttgcata tcaaaaaaac atgttacaat gaaattaatc aatggtggaa gcgttataaa
     1981 aaaattaagg agattaaaaa atatggcaag aaaaaagacg attactagag aacagatttt
     2041 ggcagctgct tatgaagtag ctgccaccga tggatttaca cgatttactg cgagaaacat
     2101 cgcgaacaaa atgaattgtt ccacgcaacc gatttatctt gagtttaaaa atatggatga
     2161 cttgagacaa gctctttttg agcaaatcta tgatcattta caatatgaag tgttcactgt
     2221 tgagcataca gggaatacga ttatcgatct tgcttttaat tatatccatt ttgcaagaag
     2281 agagaagaag ttgtatcgtt cactgtactt agaagaatat ggtggaggaa aagagatgca
     2341 gaatttctct tatacttact ttgtgaatac agtgaaacgc gatccagctt atgtgaatct
     2401 ttcagaagag cagatcgaat ctttgtataa tgggacatgg atcatagcta ctgggatcgc
     2461 agctttgatg tcttcaagca tcatccatcc aactgatgaa cagattgaaa agatgatcca
     2521 agacagtata gatgctatct tgaaattcaa agaaccaatc gaaatcgatt gaatatactc
     2581 aaaagttcca caaatcagta ggtaacaaga gatgtgtcaa aataaggaaa agttcgtttt
     2641 tcttacagcg tatgcatttt taactcccga agaactcttc catgaatgca atgaataaaa
     2701 gagccataca gattgaataa atctggatgg ctcttttatt gcaagtatat taaatttttg
     2761 cgactagttc tttagcgaaa gcttctaaat tttcgatatc ttcttcttca gcattcagat
     2821 ccactttaac attttcggct ccttttgatg cgcctgtttt tgcaaaagca gcatcgaaat
     2881 catccaccga tttgcagaat tcatcgtaaa aagtatcacc agatccgcag acaccgtaaa
     2941 tttttcccaa tagatccaat tcttgcaagt cttcataaaa gtctacgatc tcatcaggca
     3001 gatcgccatc atcgtatgta taagtagcta ctacgcaaat atctgcctcc tcgaattcat
     3061 ctgcatctac ttgggtacat tcgttgattt cgacttcgat atccagattt tcaaatgctt
     3121 ctgcaacaat atcggcgatt tcttcagtgt ttcctgtcat gctagcatat acgatttttg
     3181 ctaaagccat gatgtgcctc ctcgtttata attatttaac aattatgaat tataccaaaa
     3241 catcattgaa aagaaaataa aacctaaaga cacctgggga atgtcatttg gctaaatgta
     3301 tgccgctatg ttaaaatgag agggatttgt aaaaaggaga cgaaaatatg attactttaa
     3361 aatcagcacg tgaaattgaa atgatggcag aatctggagc tttattagca gatgtacata
     3421 aaaatctgcg tgattttata aaaccaggaa tcacaagttg ggatattgag gtatttgtca
     3481 gaaactatat cgagagtcat ggcggaattg ctgcgcaaat tggctttgaa gggtatgaat
     3541 atgcgacttg tatcagtata aatgatgaaa tttgtcatgg attccctcgt aaaaagcctt
     3601 tgaaaaacgg tgatttggta aaagtcgata tgtgtatcga tttgaaaggc ggcatttctg
     3661 attcttgttg ggcctatgta gttggagaat ctacacctga gatcgaccga ttgatggaag
     3721 taacgaagaa agctttgtac atcggtattg agcaagcaca agtagggaat cggattggtg
     3781 acatcggtca tgctatccaa acttatgtag aaggcgaaaa tctagcgatc gttcgtgatt
     3841 ttattggtca tggcgttggt cctacgattc atgaagaacc agcagttcct cattacggag
     3901 aagctggaaa agggttgcgg ttgaaagaag gcatggttat cacgatcgaa cctatggtta
     3961 ataccggaac ttggaaaatg aaaatggatc ctaacggctg gactgcgtat acaagagacg
     4021 gcggactttc ttgccaatat gaacatacat tggctattac taaagatggt ccaagaattt
     4081 tgacatcaca aggagaagaa ggcacttatt gatctatcca gtcaataggt acgggagagg
     4141 tacatgaaag tttggaacaa aataaaacaa aatgaaaatc tgatgcgctt tattgagacg
     4201 actcaaaagc gcatcactga ttcagaaatg agtacgacct ctgtcgtcgt tgcttattat
     4261 ttactcttgt ctttatttcc attgattatt gcttttggta atattttacc gtttctccac
     4321 atcgatcaag agactgtact gacctattta cgagaagtga ttcctgagac cgtttaccag
     4381 tttatcggtc ctgcaatcaa agaccttttg acacaaagtt caggaggctt gttatccgtt
     4441 tctgcattag cggctttatg gtctgcaagt caaagtatca atgctttgca aattgcgatg
     4501 aataaagcgt atggcgtaga aaatagaaaa aattttttga tcgtccgttt cttctcacta
     4561 attgtcattg tcttgttcat gattgctatt agtggggtaa ctatcgttct tgggttggga
     4621 cagctgattt tggatgctat tcagccaatc gtacaaatac ccgtgaactt tatcgatcag
     4681 ttccaaactt tgaagtggcc aatcacattg gtagctttat ttgtcatcat gttcttgatt
     4741 tatttaattg tccctaatgc tcagttgaag ttgaagacga tcattccggg cacgatcttc
     4801 gctactgtcg gctggatgct gttatcacaa gtgtttagta tctatgctag gtattttgca
     4861 acgaggatca gcggttatca gatcatagga agttttattg tactcatgct ttggcttaat
     4921 tttgctgcta cgatcatcat tttgggcgga atcatcaatg cagttgttca agaatacgtg
     4981 acgggtcatc aaatcaaaaa acgtcgagat ccaagccgaa aatggttcaa taaattgaaa
     5041 actaaatttt ctcataaaaa aaagtagaaa caagagttct tattgtaaat ttatatcagt
     5101 aatttataat aattatgact cttaattaaa gtaggtgccc atatgcttgt ttcttttagt
     5161 atggttataa gattttttct gacaatttta ttatcactga tattaacacc tgtattcaaa
     5221 atcatttcag tacaaacagg tatggtcgac aaaccaaatg aaagaagaat aaataaagtt
     5281 ccaatgcctt cagcgggcgg actgcctatc tttatttcat ttgttatatc gactttattc
     5341 ttattccgta aaattatccc tcaatcgtat attgtaccga tcttgattgc ttctttagta
     5401 atcatagtca caggagtgct cgatgataaa tatgagctga cacctaaaca aaaaagtata
     5461 gggatcttga ttgcttctct tattatttat tttgtagcag atatacgtat tgattccttc
     5521 acgcttcctt ttattggata tatccaacta ggctggctta gttttcccgt aacgattttt
     5581 tggatcttcg gtatcacgaa tgcggtcaat ctgattgacg gtttggatgg gctggcggca
     5641 gggatttcat tgatcggttt aattacgata ggaatcattg gatatttttt cctccatgct
     5701 tctacagtgt atattccagt tgttattttc tgtcttgtag caagtattat cggttttttt
     5761 ccttacaatt tttatccggc aaagatttat ttaggcgata caggagctct attcctaggg
     5821 tttatgatgg ccgtcctttc actacaaggg ctgaagaacg tgacttttgt ttcatcgatt
     5881 tctttgctga tcgtcatggg agtgccggta acagacacgt tttttgctat cattaggaga
     5941 aaagcaaacc gtgtttcttt ttctacagca gataaaaaac atcttcatca ccgactaatc
     6001 agtttaggct ttactcataa aggtgctgtc ctgacgattt atgcgatggc tttgatgttt
     6061 tctttcacag ctatggtcat gaattatact ggaagaatcg gaacgatcat attgattatt
     6121 gccatgttgt ttgctgcaat tttattattt gaattgattg gtctgatcaa tgagaaacat
     6181 cagtttattt taaaaacatt gcgattttta gggaataaag agtaccgtac gcaagtaaaa
     6241 cagaaatatt tgaagaaatg gaaccgtcat atagatgaat aacaaattaa accagccgat
     6301 ccttattagt attgtgacgg caaacagtga taaaattttt cggacactag ataattttat
     6361 ttcaactgta aaagatgaaa cagcagtaaa aatccgaatt tttgacaaca attcacttcc
     6421 cgagtacaaa aaaagactaa cagagtatag gaaatatccc tacatcgata tctattttca
     6481 tgatgaaaat tccggatttg gttatggaca taatcacaat ctgatagcta gcgattctcg
     6541 ctatggcatt atttgtaacc cagatatttt agtggatgaa gagacagtta cagcattggt
     6601 cttgttattg aaagaacatc cggaatgtgc gatgttagca cctaagattc ttaatgaaga
     6661 tggaacaact cagcacctca tccgtgagca gctggctgtt tttgattacg ttttgcgctt
     6721 tgtgcctttc aaatgggtca aaaaaaagtt tgacaaacga ctggctgtgt ttgaatgccg
     6781 aggattgcct actgatgcta atagttatgt acggatgatc tcaggtagtt ttatggtagc
     6841 tgatattgat aaatttaaag agataaaggg gtttgatgag cgtttcttca tgtactttga
     6901 agacaacgac ttgtgtttga gttttgaaaa agcaggttac aagttattgt acacaccctt
     6961 ttatgcagtt atccattttt acgggaaggg tgcgcatcga aatgcaaaac tgtttcgcgt
     7021 gtttttgact tctatgatga aatttttcaa taagtggggg tggcgttttt tctgatgaaa
     7081 ccagaaatat tagtagtcgt tgtgctttat cagcaactat tcagtcaatc tccagcttat
     7141 ggaccattaa gcaaagcact tagcgaaaag caaattcagc ttattattta cgataatagt
     7201 cctcaaaggc agcaaaatga gctgtttgaa aaagaaaata tcgtttatca tcatgatccg
     7261 caaaatcctg gtttagcggt ggcttataat tttgcgttgt ctcaagcaga tgaaggaaca
     7321 cgttgtctcg tcactctgga tcaggatacg aggctagtcg acgattattt tgaactattg
     7381 cgtaaagtaa cctttacaga tgaatgtgtt gctgcagttc cgatgatttt ttctgggagc
     7441 cgacaagttt ctcccctata ttcagatcgt tatatcaatc gtacagctca agcagtagaa
     7501 gttgaaggcc caacagctga acgaatcatg gcaatcaatt caggtgttgc ctggtctgtc
     7561 aaatttctaa aaaagattgg cgggtttaat cctgcttttt ctttagattt tttggatcat
     7621 tggctgtttt ggaaagtgga tcagttggaa aaaactgtgg agatccttcc tgtacgaatg
     7681 gaacatgatc tttcggtttt agactatgaa aaagtttcaa gtaatcgata ccgttctatt
     7741 ttacaagcag aaaatcgatt ttatcatgaa tatgatcaaa ggcatttatc cagtcaccga
     7801 cgacaattat tgttaagaac agctaaacag ttcttgaccg tcaaaaaccg gcatatctgg
     7861 cggatgactt tgcgctcata tgttaagaat tggaaggtgt gaattcgtgc gttcggtttg
     7921 tatagctacc tttaatggag gcagatattt aagagagcag ttagatagta ttttatctca
     7981 gctttcagca gaagatgaag tagtcatttc tgatgatggg tcagtagatg atacatggga
     8041 tatattaact gaatacgcag aaaaagataa gcgaatccag cttttttcgg ggcctcgtca
     8101 aggattgatc gctaattttg gttatgcgat ccagcagacg aaaggagatc taatcttttt
     8161 ggcagaccaa gatgatgttt ggttggagac aaaagtgaaa aagatcactg actattttga
     8221 gcagaatccg gatcagctag tagtagtttc tgatttaatc attgtggaca gcgagctgca
     8281 agagatccat gattcgtatt tttcgtttcg aaattccaaa gaaggatggc ttaataatgt
     8341 gataagaagt cactatattg gtgctggtat ggctttccgg tcttctttaa aacccgatat
     8401 tttgccgatc ccgtcagaag tgccgatgca tgatatgtgg attggtttgc tgggaggaaa
     8461 gaagactggg ttcctaagag aaccgttaac tctgtaccga agacatgatt taaacgcgag
     8521 tgagattgaa acacattcta aattacggca aaaaatttct tggcgtgttc atctgattac
     8581 ggcgttgatc aaacgaaaat ggtttaaacg ttgataaaag atgtttaaat ccttaatatt
     8641 tccatgacaa atataacaat tgatgagata ataacaaagt gaaatttgaa atcaaaaagg
     8701 agagcagata tccatgaagg gaatcattct tgctgggggc agcggaacac gtctgtaccc
     8761 tttaacaaag gcaacatcga aacaattgat gccgatttat gacaaaccaa tgatttatta
     8821 tccaatgtct actctgatgt tggcgggaat caaagaaatt ctgattattt caacgccaca
     8881 agatacacca cgtttcaagg aattatttgg aaacggaaat gatttaggac tgcagatcga
     8941 atatgcagta caagaaagtc cagatggatt ggcacaagcg tttatcattg gagaagaatt
     9001 tattggagat gatagtgtct gcttggtatt aggggacaat atttactatg gtggcggtct
     9061 gtctaaaatg ttgcaacgag cagcttcaaa agatacaggc gcgacagtat ttggctatca
     9121 tgtcaatgat ccagaacgct ttggtgtagt agaatttgat gagaatatgc aagctttgtc
     9181 gattgaagaa aagcctgctc agccaaaatc aaattacgct gtaactggcc tttattttta
     9241 tgacaatgag gtaatttcta tcgctaaagg tatcaaacct tcagaacgtg gagaactaga
     9301 gattacggat gtcaataaag catatttgga taaaggaaaa ctatccgtag aacttatggg
     9361 acgcggattt gcttggcttg atacaggaac acatgaatca ttgcttgaag catctacctt
     9421 tatcgaaaca attgaaaaac gtcagaactt aaaagtggct tgtttggaag aaattgctta
     9481 ccgtatgggc tacatcgata aggaacaatt gctggccctt gcacagccat tgaaaaaaaa
     9541 tcaatacggt caatatttat tgaatttggc cgctgaataa ggagtcctta cagatgaaag
     9601 taacagatac aaaattaaca gatgtcaaaa tcgtagaaat ggacgtcttt ggcgatcatc
     9661 gcggtttctt cacagaaagt tattcaaaag caaaatttgc agaacatgga ttgaaatatg
     9721 actttgttca agacaaccat tcactatcag ctgaagctgg tgtccttcgg gggcttcatt
     9781 tccaaaaagg agaagctgca cagacaaaat tgattcgggt agtcactgga gcagttttag
     9841 atgtagtagt ggatatccgc aaaggtagcc cgacttatgg tcaatgggac ggatttatct
     9901 tatctgaaca taatcatcga cagttgcttg tacctaaagg atatgcccat ggattcgtta
     9961 ctttgacacc aaatgtgaac ttcctttata agtgtgataa ttactataat gcagaagcag
    10021 atgggggcat cgcatttgat gatccagatt tagcgattga ttggccaatt gatccatcaa
    10081 gagcgattat gtcggaaaaa gatcaccgtc atccgacatt aaaagaattt gaagcagaaa
    10141 atccatttgt ttacggagaa atctaagaat taaggagaaa ttttcttatg aaaaacatca
    10201 ttgttaccgg aggtgccgga ttcatcggct ctaatttcgt tcattacgtg gtaaacaatc
    10261 atccagaagt gcatgtcact gttttggata aactgactta tgcaggaaac aaagagaact
    10321 tggccggact tcctagcgat cgcgtagaat tggtcgtagg agatgttgca gatgcagaat
    10381 tagtggaccg tctagtaaaa gaagcggacg cagttgttca ttacgcggca gaatcccata
    10441 acgataattc attaaaagat cctttcccat ttgtacaaac gaatatcatt ggtacgtata
    10501 cacttttaga agcttgccgc aagtatgatg tacgctatca tcacgtatct actgacgagg
    10561 tttacggcga tcttccatta cgtgaagacc tgccaggaca tggggaaggc gaaggagaaa
    10621 aattcactgc ggaaacacca tataatcctt caagtccata ttcttctaca aaagctggtt
    10681 cagatctgtt agtaaaagca tgggtgcgtt cttttggctt acgtgcaacc atttcaaact
    10741 gttcaaacaa ctatggtcct tatcagcata ttgaaaaatt tattcctcgc caagtaacga
    10801 gtatcttaag cggtattcgt ccaaaattat atggcgaagg aaagaatgtt cgtgactgga
    10861 tccatacgaa tgatcattct tcagcagtgt ggctgatttt gacgaaaggc agaattggcg
    10921 aaacctatct gatcggtgca gacggtgaag aagataataa gaccgtgatg gaattgatct
    10981 tagaaatgat gggccaacca aaagacgcat acgaccatgt aaacgaccgc gcagggcacg
    11041 atttgcgtta tgcaatcgat tcgacaaaat tgcgagaaga gctaggttgg cagcctgagt
    11101 ttacgaactt ccgtgatggg ttggctgaca cgatcaaatg gtacgaagaa catgaagact
    11161 ggtggaaaaa agaaaaagag gccgttgaag cagcatatgc caaaaatgga caataataca
    11221 gaggaaattt cactgtatta atggaaaaac acatgtgagg caggcgctcg cctgtctctt
    11281 ttttaaaata aaaatcaagg agcggataac catatgtttt tattaactgg aggaaatgga
    11341 caattaggta cagaattgcg ccatttatta gatgaaaaaa atctgccata tgtttcaaca
    11401 gatgcaaaag acttggatat cacagatgct gagaaaacaa tggcttatat tacagaatta
    11461 aaacccgatg ttattttcca ttgcgcagct tacacagcag tagataaagc agaagaagaa
    11521 gcaaaagaat tagatgagaa aatcaatgtc gatggcacaa gaaatgtagc acttgcagca
    11581 aaagctgtga atgcacaatt cgtctatatc agtacagact atgttttcga tggaaaaaac
    11641 aaaaatgtag aatacgaagt agacgaccaa acgaatccac taaatgaata tggtcgtacg
    11701 aaattattag gcgaaaaagc agtagaagag attttagaag actattatat catacgaact
    11761 tcttgggttt tcgggatcta tggacacaat ttcgtcttca cgatgcaacg cctagcggaa
    11821 acccgcgaca aactgactgt agtaaatgac cagtttgggc gaccaacttg gacacgtact
    11881 ttggcagaat ttatggtata tgtcatcgaa gaaaaagcac catttggtat ttatcatttg
    11941 tcgaacgaaa atagctgttc ttggtatgag ttcgcaaaag aaatattgaa agatacagat
    12001 gtagaagttg cacctgttac gtcagaagaa tttccgcaaa aagcaacacg tccgcagtat
    12061 tcagtcatga gcctgaaaaa gacagaagcg ttaggttttg tcataccaac ttggcaagaa
    12121 gcacttgcgc aaatgctgga agctgccaaa aaaatgaata agtaatttcc tttttatcaa
    12181 atagaatttg gagcatgata tggaaaaaca aacaagaagt gcacgacgaa aagaaaagcc
    12241 aaaagcgaat caggcagatt cgaatttggt gcttgacctg tttctaaaac tccgctatct
    12301 tattggcgtt ttagtcatcg tggtcatcgt tacttttaat ttaaacggaa gttcacttgg
    12361 tgtttgggat aaatatgtgt cgcaaagaga cgatgggaaa aaaactgatg tgatttttgg
    12421 acagaatcgg gaagtgcggt cagacgaatg gctggtgcag acaccttttt acctctcaca
    12481 agctgaagag gacttttcgt tggtgaacga ggattattca ttaaacggac aaaacatgat
    12541 cattgcgtat aattctcctg taaaggatat tacggttatc ggaaaaccat tcaactgggg
    12601 attctttttc ctcggaagag atcgaggact ttccttttat tgggcgatga aattagtcgt
    12661 catggtcttg cttggattcg aagtactgat gatattaacg aagaggaaaa aagctctatc
    12721 gctaatcggt gcatttgcgc tgacattttc tccagcagtc cagtggtggt tcatgcaaca
    12781 tgtgggcgat ttgattttct ttactttggg actgatgatt gctttttatc attatttcta
    12841 caatcatgat aaaaaatggc tacgaacgtt gatgatgctt ttggtggtta ttttcggtat
    12901 tgggtttatt ttagtgattt acccagccca tcaagtgatg ctggcgtact tgctgatttt
    12961 ttattttgtt ggcctgctta tttactatag caagaaagtt tcatgggatc tgttggatgt
    13021 agtgttgatt ggaggggcag ttttatttat tggcggagtc atggttcatt tctggctaac
    13081 gtcaaaagac gcattgttag cttctttgaa taccttatat ccaggaaaac gggtaagtac
    13141 tggaggaaag tggacgattg gcgaattttt ttctttcctt acaaattgga aaattccttt
    13201 tgaagatatc aactattcga ataactctga agtggccctg ttctatcact tttttccaac
    13261 tgtattccta gcaagtccat ttgtgttatt tggcaaaaaa gatagcgaac aaaagttact
    13321 gggacgtatt ttgatgattt tttgtctgtt ttctatcttt tggattaccg ttgggttgcc
    13381 aaaatggcta gcagaaatca ctctgctgtc ttatgttcct ccattaagag ccatccttac
    13441 ttttagtttt gcggcttgtt tattatcgat ctggtttatc gcttatattt ggagagaaga
    13501 tgttattcct atttggtata aattcatttt gttggcagta aatgcatggt cttatttcta
    13561 tgcgattacg cattcgaaaa tggataatta tttttcggat tttgaaacca tcgcagttgc
    13621 tgtgttggga acagtgattt tagcttttgc attatttggc tggagacgct tgttttacct
    13681 cgcttttgtc gccttagtta tcgtatctgg ctttttcgtc aatccagtcg tagaaggtac
    13741 aggagcgatt tttgaaaaaa cactggcaaa agaaatccaa aaaatcgatc aacaagatcc
    13801 tggtaaagtt tggttgtctg aagatgaatt atataacttc accccaacat tgggagtgaa
    13861 atccttcaat acagtacgct tttatccaga tatagctgca tggaagaaaa tcgatccgaa
    13921 agaagaatac gagaagtatt ataatcggta tgctcataca agagctttta tcgctaagga
    13981 agatacaaag ttcattttag atcgtccaga tatgttttcg gtcacgttga attttgaaga
    14041 cgcagaaaaa ttagggatcg actatgtgat cagtaaacga ccattaactg attacaacca
    14101 attatatgaa gcgcagttca cgcagctata tggaccggat aaagacggtt atatgatttt
    14161 taaagtagaa tatccggatt atccagatgt accacaaaca caaactcagg aaaatcagct
    14221 agtcgtacag taataggcta gctgagaata atcctgaaat attagtagaa aggagagtga
    14281 agataattca agcaatcgta caaaaatttg tcaacaaaat gagtgtcggt ttcttgcttt
    14341 tgttgctgac gctagtcttt tttgtttgga cagcaggggt aggttttcca gcaactttgt
    14401 ttatatataa caatagttca ttacttatcg cttttagtgt ggctattttg ctcattttaa
    14461 atgtttataa agcaaaaggc gtcgattttc tttatctagg tgttgcgctt gcccttttca
    14521 ttttcttttc ttactttcgg gaattaagaa atgagtcttt atttggagac ttactgtttc
    14581 ctttgattat tttattttgt ttagtaatta aatggatcga gtggacaaaa tgggatcgcg
    14641 gtatttatct gattgtcttt acggtcttcc tcggaatcac gttatatcgt gtatttacag
    14701 agatccaagt accagaagga caaagcatct gggctccagg caattcgctt tctgatattt
    14761 ggatcaatac gaatacaatc ggaagttctt tgatgactct tgctttattg atcagtggat
    14821 ttgccagttc ttttgaacga tggtatgttc gaatcatcgg tgggatagca gtcgttgcag
    14881 cctttttagg tatttgggtc tgccgatcta gaggtgcatt agtcgcgttg attgtttttg
    14941 cgatattgga tcttttacct aaatcgttga tgcgtgtcat tcgtgcgcca tttattgcct
    15001 acacggttac gattctttta gctttgccga tttcttatct ggcggctgtc tctgaaaaag
    15061 tcaatttgtt taccggtcga gaagatattt ggcataaatt ctatcagacg ctaggagaaa
    15121 aatctgaaca agttttacta ggaatgaaaa catttatctt ccagcgagga aaccaatttt
    15181 taggaaacca caatagttat aattctattt tgaatatcta tggattgata ggatttggga
    15241 ttgctgcttt gctgcttatc ctttttattg gtcgcttaac gttgaaagca gatttatcca
    15301 acgggcagat gacttttatc tgggcctttt ttgcggtgat gatgcagtca tttatggagg
    15361 atacattgac ttcataccca tgggttccga ttatttatat cctgttggct atggccgttc
    15421 atcgatatga tgaaccagaa gttacggagg aattctatgt tatggaagag gcatatgacg
    15481 atatgactta tgatgataca tcttatgagg aacaaccaga tagcgctcat tcttccagga
    15541 tccaacgcta tcattgatag gcacaaaata cgagagaggc tgtgacaacc gtcgcagcct
    15601 tttttggttt attgcaattg tctagcatct tctgtaaaat agaaacgtta gagaaaatct
    15661 tgtatcatag aaaattaaga aagaaataag aacgcagctc ggtaccaaaa atatggtaaa
    15721 atggcacttg actgtaattc actgaaaatc gcttggagga acacaaatgt ttaaagattt
    15781 gtatttattt atgaaggaca tttatcagaa cagaaaatta ctgctacagt tttcgatcaa
    15841 tgacttcaaa tcaagatatg caggatcgtt tttaggaatc ttatgggcgt ttatcaatcc
    15901 gctatttact gtattgattt attggctggt atttggtgtt ggactgaaag cacaaatgat
    15961 cgatgcaaaa tacccattta tcgtatattt agttaccggg atgattccct ggttcttctt
    16021 ctcagatgca tttgcatcaa ctacggttgt ttttcgggaa tatacttatt tagtaaagaa
    16081 agtcgtattc aatattcgta tcttgcccac gacgaaaatc ctatccaatc tgtacaccca
    16141 tttattcttc attctgattg gcatagctgt cgcgcttata aatggtatat atccttcagc
    16201 aatgtcttta cagctgattt attatttatt ttgcatgtgt gctttcttga caggattgac
    16261 ttggctgacg gcatcgatcc aaccattttt acctgatatc atgcaattaa ttacgatcgt
    16321 gctgcagttg atcatgtgga cattaccgat attatggagc cctaatcagt ttgattcagg
    16381 tcttgtaagc atattgaaat taaatccgct gtactatgtt gttcaaggtt atcgtgagtc
    16441 atttttaagt gaagcttggt tctgggagca tggaacatat actttgtatt tctgggtttt
    16501 cacgttagta atgttcgtaa tcggctctgc agtgttccgt agattgaaac cacatttttc
    16561 agatgtgctg taaggagaat agctatgaca gaatatgcaa ttagactaaa agatatcaca
    16621 aaatcttata atatgtacgc aaaaccttcg gatcgatttc gtgaagccct caatcctttt
    16681 aagaaatcct atcatgatgt attttatgcg ttgaaaaacg tgaatgtaga aataaaaaaa
    16741 ggtgagatga tcggtttcgt gggagaaaac ggatcgggaa aatctacaat gttgaaaatc
    16801 attacgggag tactgacacc aacatccggc acgatggaaa tcgaaggaaa aatctcagct
    16861 ttgctagaac taggatcagg attcaatcct gagtattctg gctatgaaaa tatctattta
    16921 aatggaatgg ttctaggatt ttcaagagaa gaagtcgatg aaatggtcga tgatatcatt
    16981 gaatttgctg acatcggcga ccatatttat caacctgtaa aaacctattc aagtgggatg
    17041 ttcgttcgtc tagcttttgc tgtagcgatc aatgtgaatc ctgatatttt gattgtggat
    17101 gaagcattgg cagtaggaga tcttgaattt caactaaaat gtatggaaaa atttacagaa
    17161 atcaagaact cgggaaagac gattttattc gtttctcatg atattagttc gattcgccgt
    17221 ttttgtgatc gttcctattg gttgaaaaat ggaaaagttg tcgaatatgg tgatacgatg
    17281 gaagtaacga cgaattacga aaactttttg aagaaaaaat cagttaaaac agtagatcga
    17341 aataaaaacc aagtagaacg cttcgcagct ccagatatcg tagacgtgac gaaagccgaa
    17401 ttgttggata aagataaaaa tccagtggat atgctggaac aaggagaaaa actatacgtt
    17461 aaagtaacct atgaagtaaa agacgataca atcaaaaatc cagttttagg aattgctctg
    17521 cgtacagtcg acaattttta tgtgtgcgga ctcaatactc tactagatgg tatcaagatc
    17581 ccttggaaaa aaggtgagaa tatcttttat ctggagtatg aaaagttagg tttgttagct
    17641 ggtgaatatt attttgacgt tgcggtcttt gaagaaaatg caacggttcc tttggtatac
    17701 aagacaaaat acatgaactt gtttgtcagc ggttcttata tcggagaagg catcgtggtc
    17761 ttagaccata aatgggaaga agggactcat agcaatgaaa tatgattttg aaatggacct
    17821 agatgaacaa agcagtgtgg gaaaaattgc cgcacagatc aaaccaggaa gtaaagtttt
    17881 agagtttggt cctggaaacg gccgattaac gaaacatttg atcggagcaa aacaatgtga
    17941 agtgagcatt gtcgagctag ataaggaatt gttcgatttt gtcagcgagt ttgcccaaga
    18001 tggattttat ggagatatcg aaagttttga atgggcaaac tattatgctg gacaaacatt
    18061 tgattacgtt ttgtttgccg atgttttaga acatttggta gatcctggaa aaacgttgaa
    18121 gaaagttaga gaatttttga atgaagaagg agaaattcta attacattcc ctaaccttgc
    18181 gcataattct gtcatgattg atttgttcaa taatcaattg ccatgggctt cttatggttt
    18241 gctagatgaa acccataatt ctttttatac tcatgatggc ttccaaaaag tttttgaaaa
    18301 agcaggatta tacatcaata ttgaagatta tttgtactta gctgtaggtg atacagaatt
    18361 aaaatcaact tatgaagaat tgccggaagc tgtacgttat gatttcaaaa tgcgaccatt
    18421 tggcgaagtc taccaatact tcttctcatt gatgaaacat ccagtagcac aaagttccat
    18481 tgcgcagccg caaaattcta attatgtgaa agtattagaa gtaatccagc aaacgaaaca
    18541 agatgaaata attcaacaaa taccattcaa taatttcaca ggggaaaatg agacactaag
    18601 tttccatgta tcagaattag cagaacgaat ggtgtttcgc ttcgcgcagc aacctagctt
    18661 tatcgaattc agcgcagaag ctgctggaga aaaactcaca tttatcgatt caaatgctgt
    18721 agtcaaaaca gtaaatgatt gttatttgtt tgatggtaaa gatttaccag agttcttttt
    18781 aacggatatt gcagggaaag aagtgactat ccattgtcac tatcgtttta tcggagaatt
    18841 gacaccaaca atgaaagaat tgctggaaac aatccgtcca atggctgaag ttacaaagca
    18901 gctttctgag aaaaatgatg aattagaaag agaaaatcat tatcttattg agaataatac
    18961 aagattagat cagacgttga aaacaaccat aaatcgctat tgcacgttgt tggaaagtga
    19021 tgaatggaca atcaaacata gattagggcg acgtaagaaa gaaacatcga aaaaaatcca
    19081 agaaaaagag ctttctctat gtattgacga aaaaatctgg gatgcagaaa caaagatatt
    19141 gaaaatcatc gggtggggaa tcgctaattc ggatcgccaa ccattgtcct ataagttatc
    19201 agccgaccaa tcaccatttt ttgaagcaat tcctgtttta cgagaagaag tcaatcaagc
    19261 aaaacagcta gctgaaggag caaaagcagg atttgaactg cggatccttt gcgagaagga
    19321 acgttcattc ttagttgaag cagtggctca aaatggtcaa tcatggatca ttgaaatgta
    19381 aataatgatt aacttataaa gataaggagt aaacgaatcg tgcaaaaaaa agaaattgcc
    19441 gtatttatcg atcaaatcac tcgtgaacgg gccactggcg atttgcttat tgtgggctgg
    19501 gcaattgatg aagtgaccaa agaaatccct acgataaagg tagaaaaaga aaatgtgatt
    19561 gctgaagcaa cacatgtcgt aagattggat atcaatcatt tatataatct tgatgttaag
    19621 acccaaagcg gttttaaggt acgtttatca gggaaaatga gaggaaaagc aatcttagac
    19681 ttccaaacag caagacatca aaatggcata gctgtgaaat taaacggtaa atatccttat
    19741 gatgacggca ttgaatcttc atgggaaaga aaaaaaagat tactgaaaaa agggataaat
    19801 tatgcaagga ctcatggcgt aaaaaaagca atccgccggg tcaaactgga attgaaacca
    19861 ggatcgatcg actatgcaga gtggatcagt cgtcatgaga aacctgatct gaaagagcaa
    19921 aggaaagaag tacaaggatt tgcctaccgc ccattgattt ccatacttat gcctgtctat
    19981 aacgttgaaa tcaaatggct cgaaaaatgc attgattcgg tacttgatca aacgtatgat
    20041 cactgggaac tatgtatcag tgatgatgct tctactgatc cagctatccg aaagtgtcta
    20101 gaatcctatc aagcaaaaga tgatagaatt aaagtcgttt tccgacaaga aaatggacac
    20161 atcagcctgg caactaattc tgcattggaa atggcagaag gagagtttat agcgctctta
    20221 gataatgatg acgaattacc tccatttgcc ttatatgaag tggcaaaagt gctaaacgta
    20281 catccagaac tggatttgat ctacagtgac gaagataaaa tcgatgcaga tgggaatcgc
    20341 tttgacccgc acttcaaagc agactggtct cccgatacat tgatgggaaa taattatatc
    20401 tctcacttag gcgtatatcg gacaagcatt gttaaggaac ttggcggttt ccgtaaagga
    20461 tatgaaggct ctcaagatta tgatttggtt cttcgtgtga cagaacaaat tcctgcagaa
    20521 cacatttacc acattgaccg tgtcttgtat cattggcgga cgatcccggg atcaactgca
    20581 agcaatggag aagctaaaag ctacatctat gattctggag tgaaagcatt gacagatgct
    20641 ctatctagaa gaaatatcaa gggaagcgta catcctggca gaatttctgg tttttatgaa
    20701 atcacttatg atgttttgca agaagacttg gtcagtgtga tcattccaac taagaatggc
    20761 tatgaagatc taaaaacttg tgtggattcg atcatcgaaa aaacgtctta tccaaattat
    20821 gaaatcatta ttgcagataa tggaagtaca gatcctaaaa tgcaagaatt atttgcagaa
    20881 tacaagcatc agctgaaaga ccgatttatc gtcgaattaa ttgacattcc attcaattat
    20941 tcacgtatca acaatctggc agcagaaaaa gcaaacggca aatacttttt attcttgaat
    21001 aacgatacag aagtgattga acctgactgg atgacagcga tggtttctta tgcacagttt
    21061 gatcgaatcg gctgtgtagg agcaaaactg ttttatccag atgatacaac gcagcatgca
    21121 ggagtattat taggaatcgg tggtgttgca gggcatgcat tgaataacta tgatcgaaca
    21181 cattgtggtt atttcggccg attggtcatt gacgtcaatt acttagcagt cactgcagct
    21241 tgtatgatgg tcaaggcagc tgacttcaat gctgttaacg ggtttgatga aacattagaa
    21301 gtagcattca acgatgttga cttgtgcttg aaagtttatg aactgggtcg ttacaatgtc
    21361 tatgcacatc aagcagaatt gtaccatttc gaatctaaat caagaggtta tgaagatact
    21421 ccagaaaaac aaagacgttt tgctggtgaa atcaaaaaaa tgcaagataa atggccagca
    21481 tatattgcgc atgatccatt ttacaatgat aatttaacaa aagaaggaat cggcgacttt
    21541 tcgttgagac cagattaaac tataataaaa agctgttatg ttgtgattcg ctcgcacata
    21601 acggcttttt atagtttttt gtcaaaaaaa ggcagaaggt gccgactcaa aaaaataagt
    21661 cgaggttggg acagaagtgt ttaactccga gaaataagaa gaaattcacg aaaattgctt
    21721 ttcaaatttt tgtgaatttc ggcttattta agaagaagtt gcttctgctc ccaccgttta
    21781 ttcgttttta aagaatgaga caaaagtgtt tcttactttt gtcccacgct cttattcatg
    21841 ctcagagcgc caatagatac aaaatcatag ccaaagtttc acgaacccta gctgtcaaag
    21901 aatcgatatt ttattttaat ataggctatt ttaaaataaa tggtttaagg tattcctcta
    21961 caaaagccac tctatcaatt atcatgggtt taaaataaga agtgatggag ataaccagat
    22021 tgttcttagc gtcaatataa agaacatttc cactattacc aattgctgaa tatatttcgt
    22081 tctcagaatc taatacccac cataaatagc catatctcat attttgtaag tcttctaaag
    22141 cactggttct aggtttgaac atctcgttta tccacatggg tggtacgatt tgttttcctt
    22201 tatacatccc tttatttagg cataatacac caatttttgc cagatcactt gccgataaac
    22261 acaaaccata tcctgccgtc ccgatattta gtggatcaca aaaccagaca ttggttttag
    22321 gagacttatc cattgtaaac tgcttatgtt cttcagctgt ttcagcataa taattgacat
    22381 ggtttggtat ccctatagga tcaaatagat acttattggc aaaatcaaca gttgttagct
    22441 tacttgtctg atacaacaag ccagttaata tgtgtaaaca gacagtttga tagttgaatt
    22501 cgccggttat accttttctt cctcccaata aatctagtga tgtttttgtc caatcttcac
    22561 ttgaacagac ttttgtccat ggatcgcctt tacatttgta gggtgctttc attgttagca
    22621 agtgcttgat tgtaatagcg taaatagttt tctccccgcg tttcacttta tactctggaa
    22681 aataatctaa tactttatca tcgatacttt tgattaatcc ttgctctaaa gcaatgccaa
    22741 tcagcaaaga tacaatactt ttcgttgctg acatgatatg acaagtatcg tcttttttat
    22801 aaccattcca ttcatctgta taaacttcta ccccatcctt atatgcgaca atttgacata
    22861 tatttggttg tcgaaaacca ataaaatcat gcaactcttg tttattcata agatattacc
    22921 tcactttctt ctataagcat aatgtcatta aaaatcgatt tcaatttttt cgattcttca
    22981 cgtaatacag gtattcttat ggactagaca gcgctttggg aagatggatt ctctgaaaaa
    23041 tataaaggtt cactttatga gtggataaaa aaagcagcaa atacttcgac ttctttaatt
    23101 taccaacatt agtgaaaatc gattcatctg gagatttgta atttagagtc cttgtcttga
    23161 ttagaggtgt ataaagcttt ttattagaca tttttaaatt ccattacaat ttgcttataa
    23221 actaggtttt taaaggtgac tatggtaaga taatagagat attattttgt gattaggagt
    23281 gtatatatga ataaaaatgg agaatggagt gctgcacgac ggatattcat tatattaatg
    23341 gatgttattg tatacaatgc agcaatatat agtagtttct tactgaagtt tcgtggagag
    23401 atcccattac gaaatttaga aactttccaa cactcagcaa ttattatttc agtgattttt
    23461 attgcgttga acattttgtt gggtgcatat gttttttata atcgaatgat cagtgacatc
    23521 gtgtttgtca cagtcatcgg tcaggtattg atgacactag ggatcatggt agtgactttt
    23581 gcgggccgct ggtttgcttt tccacgtctt gttatcttac tttcatttgt tctcggtacg
    23641 cttttcttaa gtatctatcg tattctcgta tataaacttt atttacgcgt aagtcgagat
    23701 aaaaaggtcg tcattattgg gttagaaaag gatgtcgcac cagcaatcca aaattttggt
    23761 tcaaagaaaa acaacaaaca taaagtcgaa gcagtcattc ttgataacta ttatgcaaac
    23821 atcaaaaata tgattaaaga catcgatatt gtctatcttg cttctcatat cgaagaaact
    23881 gaaaaaatca aaatctatca attagttact aaaaatgaaa aaaaattgtt tttgaataca
    23941 acatttgaaa acttgacatt gatcaatcca aatattatga atttcgaaga tgaaagtatt
    24001 attgaagcat ctggatttag aatccaacct gaatatgaac tattcaaacg tttgtttgat
    24061 atcatcgttt cacttgtttt attagttatt gcttcaccat ttatgttatt cacagcaatt
    24121 ttagtcaaag tgacatcacc gggaccaatc atctataaac aagtgcggat cacaaaaaat
    24181 caaaaggaat tttcaatcta taaattccgc tcgatgaccg ccacagccga agcaaaatct
    24241 ggaccagttt tagctaagag caatgatgca cgagtaacgc ctgtaggtaa atttattcga
    24301 gctgtccgct ttgatgaatt accacaaatc ttcaatgtgc tgaaaggcga tatgtcgatc
    24361 gtaggaccac gtcctgaacg tcctttcttc gtcgatcagt tcaatgcaga aaacccttat
    24421 tactacttac ggcataatgt tcgtgctggt atcactgggt atgcacaagt ctatggaaaa
    24481 tatgtctctg attacaacag taaattacgt tttgatttac tttatatcaa aaaatattca
    24541 ttgatgcttg atttaaagat cttgctgcaa accatcaaga tcctatttga taaaatgtcc
    24601 tctcaaggac ttgaagaaga cgaaaatgtg atctcattgg cagaaattgc ttttccgaat
    24661 gaaaaaatct ttcgctagtt gggaggcgta aaaatgaaca atccgaaatt cagcataatc
    24721 gtaccagtat acaatgcaga agcaactatt ggtcgaacga ttgatattct aaaagaattg
    24781 gattatgaag agtacgaagt cgttttagta aatgatggat caacagatca aacacaggaa
    24841 atcattcaag aactgattga aaatgatgaa cgttttgaat tgattactac gaccaatcaa
    24901 gggcccggat tagcaagaaa cgaaggaata agacagtcaa aaggagaata tctcttattc
    24961 ttcgatgcag atgacacgcc agtaaaaacg atcttgtcag attatgatca tttgctagaa
    25021 aagagtccaa aagcggatct tgttgttagt tcgtttgttt ttcgtacaat ggacgaggga
    25081 agaatcgttt ccgaaaaaga aaacttagtt acagaatgtc actatctaac tcacgaagag
    25141 tttataaaag acatgtatca gttaatgaat gaacagttga tgtatgttgt atggaacaaa
    25201 tgctatcgta gcgatattct gaaaaataac caaattctat ttaaaactta taatagttgt
    25261 gaggatcgaa tatttaactt acattattat gaatactgtc atcaagtgat catgaaccca
    25321 aaagtagaat atatttatga atttgaaggt gggaagggga tcactaatca ataccgacct
    25381 aacaagttcg aaacatttaa agaattctat tcattagcaa atactgtaac agacgatatt
    25441 aataaagacg gtatggcggc gcttctttta aaagggacaa cttcagttat tttttctatt
    25501 tatagtacct ctgcaagaac ttcaaaagag aagaaagacg aagcaaagca gatattgagt
    25561 gatccagtga tccgagaagc caagcaaatt gctaagacgg attcttcagc aaaaaaaata
    25621 acaaagttgc tttacaatat gccagctcca ctatttttat ctgctgtaaa agttggaagc
    25681 ttcgttgaag tgaaaatgcc agggttaatg gctgctttga agcgagtata ttagttaatg
    25741 gaggaaaata taatgaaaag aattttgcat tttcagggac gaatgggttt aggtggagca
    25801 gagtctttca tgatgaattt atatcgtaaa attgatcgta caaactatca atttgatttt
    25861 ttgatttatg aagattatgc agatgttcaa gactatcatt cagagattga aagattagga
    25921 ggccgtattt ttgttgtacc taatcctaag aaaaatattt ttaaatattt aatagaagtg
    25981 aataagttac taaaaaaaga atctttttct atagttcaca atcaggttta ttttggcggg
    26041 ggaattaatt tatggttagc caaaaagaat ggaatcagac agagaattgc tcatagccat
    26101 gcaactgaag atggaaaatc tcaaaatatt gtaatgaatg tattgcgtaa atttttaacc
    26161 aaattgttgt tacaaaatgc aacagactac cttgccgtta gtcaagaagc tggtgaaagt
    26221 ctgtttcaaa accatccctt tgagatagtt cataatggta ttgatttaga gctttatagt
    26281 aaaaattcag aagctaaggt taataagaga aaagaattgg atatctcaat gagtactttt
    26341 gttgtgggaa acattggacg attagagaaa caaaaaaatc aatcttattt gttagagatt
    26401 tttttagaaa tactaaaaat gaattctgat tcttacttga ttttagtagg tgaagggtca
    26461 cttagaaata aactgaaagc caaagcagta gaattggata tagagaaaag agtaaaattt
    26521 ttaggagaaa gaaacgatat tcccgagtta ttatctgtga tggatgtttt tgtaatgcct
    26581 tctctttatg aaggtctgcc aatggttgga ttagaagcac aagcagcaaa attaaaatta
    26641 gtcttagcag atactatttc caaagataca aagttaacaa ataatgtgga ttttgtatca
    26701 ttagatgaaa caccacaaga atgggcaaaa aaagtaataa aagagccgta tgctaataaa
    26761 ataacacaag aactaaagag ttatgatgtt tcgtacacta ctga
//
LOCUS       DACDIG010000036        25692 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.036, whole genome shotgun sequence.
ACCESSION   DACDIG010000036 DACDIG010000000
VERSION     DACDIG010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25692
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.036"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(60..605)
                     /locus_tag="IV247_001566"
     CDS             complement(60..605)
                     /locus_tag="IV247_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8777641.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(617..1309)
                     /locus_tag="IV247_001567"
     CDS             complement(617..1309)
                     /locus_tag="IV247_001567"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAP8777642.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1328..1612)
                     /locus_tag="IV247_001568"
     CDS             complement(1328..1612)
                     /locus_tag="IV247_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777643.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1625..2188)
                     /locus_tag="IV247_001569"
     CDS             complement(1625..2188)
                     /locus_tag="IV247_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP8777644.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2416..3078
                     /locus_tag="IV247_001570"
     CDS             2416..3078
                     /locus_tag="IV247_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAP8777645.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3202..4398
                     /locus_tag="IV247_001571"
     CDS             3202..4398
                     /locus_tag="IV247_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAP8777646.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4499..5875)
                     /locus_tag="IV247_001572"
     CDS             complement(4499..5875)
                     /locus_tag="IV247_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8777647.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5912..6778)
                     /locus_tag="IV247_001573"
     CDS             complement(5912..6778)
                     /locus_tag="IV247_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAP8777648.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6867..7892)
                     /locus_tag="IV247_001574"
     CDS             complement(6867..7892)
                     /locus_tag="IV247_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8777649.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7910..8584)
                     /locus_tag="IV247_001575"
     CDS             complement(7910..8584)
                     /locus_tag="IV247_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8777650.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8768..9661)
                     /locus_tag="IV247_001576"
     CDS             complement(8768..9661)
                     /locus_tag="IV247_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8777651.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9735..11432)
                     /locus_tag="IV247_001577"
     CDS             complement(9735..11432)
                     /locus_tag="IV247_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP8777652.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11425..12738)
                     /locus_tag="IV247_001578"
     CDS             complement(11425..12738)
                     /locus_tag="IV247_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP8777653.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12738..16256)
                     /locus_tag="IV247_001579"
     CDS             complement(12738..16256)
                     /locus_tag="IV247_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP8777654.1"
                     /translation="MDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEK
                     KEISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMV
                     NFYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETL
                     YKDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDV
                     LFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGS
                     SNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAI
                     GRNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVS
                     PFGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYK
                     FSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSS
                     SIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFET
                     KMPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEY
                     TSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNI
                     NKLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDG
                     KYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRL
                     YDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTL
                     DGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQP
                     LHLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIID
                     GVLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPND
                     SSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTL
                     KVEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYIL
                     LAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16549..17007)
                     /locus_tag="IV247_001580"
     CDS             complement(16549..17007)
                     /locus_tag="IV247_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8777655.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(17304..17822)
                     /locus_tag="IV247_001581"
     CDS             complement(17304..17822)
                     /locus_tag="IV247_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP8777656.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(17824..18441)
                     /locus_tag="IV247_001582"
     CDS             complement(17824..18441)
                     /locus_tag="IV247_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP8777657.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(18438..19292)
                     /locus_tag="IV247_001583"
     CDS             complement(18438..19292)
                     /locus_tag="IV247_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8777658.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(19289..20200)
                     /locus_tag="IV247_001584"
     CDS             complement(19289..20200)
                     /locus_tag="IV247_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8777659.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(20241..21575)
                     /locus_tag="IV247_001585"
     CDS             complement(20241..21575)
                     /locus_tag="IV247_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8777660.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            21783..22613
                     /locus_tag="IV247_001586"
     CDS             21783..22613
                     /locus_tag="IV247_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAP8777661.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(22857..23106)
                     /locus_tag="IV247_001587"
                     /pseudo
     CDS             complement(22857..23106)
                     /locus_tag="IV247_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(23297..23491)
                     /locus_tag="IV247_001588"
     CDS             complement(23297..23491)
                     /locus_tag="IV247_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777662.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(23996..24181)
                     /locus_tag="IV247_001589"
     CDS             complement(23996..24181)
                     /locus_tag="IV247_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777663.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(25031..25372)
                     /locus_tag="IV247_001590"
     CDS             complement(25031..25372)
                     /locus_tag="IV247_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP8777664.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(25372..25602)
                     /locus_tag="IV247_001591"
     CDS             complement(25372..25602)
                     /locus_tag="IV247_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP8777665.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
BASE COUNT         7626 a         5501 c         4378 g         8187 t
ORIGIN      
        1 gttttatgct cttcttttat tattttgtgc tttttatttt atcaaatcac tttgtttttt
       61 tataaaattt ctgcgcctag tgtggactca aaatgtctca atgcccattc atgtccaacg
      121 ggatcgaaac ttgccacggc atctttgaaa atatggagtt tgtatcctag attatacgca
      181 tcgactgctg tatgcaacac acagatatct gtacatacgc cggtcaaata aatatctgtg
      241 atctggcgtt ctcgcaaacg gatgtctaga tctgttccac taaaagcgga ataatgccgt
      301 ttgtctatcc aataaacatt ttcctcttgc tcatagtctt ggtataaact ttttaaagag
      361 ccgtacaatt cccgtccacc cgttcctatc acattatgcg gaggaaataa gcggttctcc
      421 ggatgaaagg aatcttttgg atcatgcgca tcaatggcaa aaacgacaaa atccttttga
      481 tcaataaact tctttgtatg agcagtcagc tttttttcaa ttacttgtcc tgctgtccca
      541 gtagtcagct ttccatcctc tgcaacaaaa tcatatgtgt agtcaatcga gatcaatgcg
      601 ttcatcttag tcctccttat acgagatgtt tgacaaattc gatcaccatt tctgctgatc
      661 gtttgcctgc gtcttcgata aattcgtcaa agctttgtgt cgccgagtga tctgctgtgt
      721 cgctcattgc acgtacgatc aaaaatggga tattgaactg acgagctgtt tgtccaacag
      781 cggctccttc catttcgcaa gctaatgctt cagggaaatt cgttaagatt tctttgattt
      841 tatctggcga gtcgacaaat gtatcacctg taacgatcaa tccttctttt gcgttcaaat
      901 tcgttgcttt tgctgcttta accatttcac tgcgcaaata tgtgcttgct tcataatata
      961 gaggcattcc tggtaattgt cctgggttgt agccaaatcc tgttgcatct gcatcaaaat
     1021 aagctacttt gtctgatata acgatgtcac ctacttgtaa gccttcaccg attccgccag
     1081 ctgaacctgt attgatcacc atgttcacgc cgtattgttg gatcaacaga cttgtggtaa
     1141 tagatgctaa gacttttcct ataccggaac gtacgacgat cacttcgtgg ttacctaatg
     1201 aacctgaaat aaataaagcg cctgcgcgtt cccatgataa tggttcgctt aggttctctc
     1261 tcaaaatttt tacttcttct tccattgcgc cgataatacc gattttcatc tttttaattc
     1321 cttctttcta tataaatgcg attgctaaaa aaaccacaca taacattagg ataacaataa
     1381 taagagattt catcaaaaaa gagttccatt tggtcgtctt ttcccgttcg ctgatccttg
     1441 ttttcgtgat tggtttattc tttttgcttt ctttacgata aaagctggca atcttttttt
     1501 cttcttgctg ataagctgtt tctgcttttc gctgtttttt taatgactcg cttgcttgtg
     1561 cttgttgtcg cttgcgaagt tcgcttcgcg taataagcgg tcctttggcc ataagccgat
     1621 ccctctactt tcctttggtc aatagttccc agtattgaat cgcataaatc gtctttgcat
     1681 cacaaatcaa ctgatctttc atcgcttgtt gcgcctcttc aagagttaga tgatacaact
     1741 ccagtacttc gtcttcatct tgtgctaaag gattctcgac cttctctacc ccttgtgcat
     1801 gataaatatg aaggacttca tttgcaaaac ctggtgaaag atacatcgaa gtaagatggc
     1861 tcagcgactt cgcacgatag ccagtttctt cttctaattc tctagctgct gtcatttcgg
     1921 gattttgtcc ttctcctgga tcgatttttc cagctggaat ctccaaaatc actttttcta
     1981 aaggttttcg aaattgtttg acaagaagca gacgatcttg ttcgtcaaaa gcaatgatcc
     2041 cgacaccgcc aggatgaaat accagttctc tcttcgaagt tcctccatct ggcaaacgaa
     2101 cttcgtctac agcaacatca ataatttttc cgctatagat ttctttgcga ctgattgttt
     2161 tttcttcaaa ttgctcattg ttcagcatat cttctcctgc tttctttgta atcataactg
     2221 cttttttttc atttttcaat ttttctcaaa aactccttat tagtttacca tagataaacc
     2281 ttagattttc tctaaatata gaaaatcctt ctttagtccg atactcttta tagtagtccg
     2341 tatgctaaaa taagtatgta atcgaataac gaaaagaatt caatactcaa cgataaatga
     2401 catggagggt ttattatgat aaaacgaaat tggccgtggc ttctagcatt ggtcttgcta
     2461 tttctctttg tccgaaacaa tagtttttct tttgttctag gacttttatt gataggtgta
     2521 gttttagttt tgtggggact ctttggttcg cgcaggaaaa agggaaaaca agaagaaatt
     2581 cctgcactga ctgacgaact tgaaactcac tatgaaaaaa gcggtatgac agcgaatgaa
     2641 atttcatttt ttcgacaaac aatgaatcaa acaaaaaatg agatcaatca attgcaaatg
     2701 aatatgcagc agacagccaa acttaaagca atcgatctcc gccatgatcc agtcaaagca
     2761 gcgaaagctt tattcaaaga attggtgaaa gaaccaaacc gactgcatga agcaagtcag
     2821 tttctttata cgcatctgcc gaatctagtt gatttgacaa ataagtataa tgagatcaat
     2881 gaccatgaga tcaaaaacaa acagacctat gaaaaactag aagaaagcgc gcagatcatc
     2941 gaccagctgt cagctttaat cgcacaagat tatcaaaaat tcgttgctga ggatctagat
     3001 gatatcgacg tagagatatc tgttgcgaaa cagagcttaa agcgtgataa taaatatgaa
     3061 ccagaacaaa aggattaact cttttgttca taaaagaaga ctcggaacat tcagatgatg
     3121 tctggctgtt tcgagtactt tttctaaagt agagacattc atgtaagaat cgagaacaga
     3181 ttcaacaagg aggaattttt tatggatcat aaaccgactg aaacaaaaga tgttacacca
     3241 gttaacgata cacttgaaga tttattgaac aacccgtttt caactccaac agatacgctg
     3301 accacttcac aacaagcgga gattgatcag ctccaagagc aacagacagc tgctcgttta
     3361 attgacaaac taccggcgga acgccaagaa caagctaaac agctagcagc aaaaatcgat
     3421 gctaatgatg cacagtctgt catcagctac ggatcggctg cccaagcaaa attaagtgaa
     3481 ttctcacaat cgatgttgaa tcatgtgcaa gcacaagata ttggtcctgt aggagattcc
     3541 ttaactgaac tgatgtatcg tctccaagaa gccaatcctg acgaactgcg tgccggagaa
     3601 gggaacatct tccagcgtgt cttcgggaag gtcaaacaat cgatctatga agtgacagca
     3661 aaatatcaaa agattggtgc acaaattgac aagatttcag tgaaattaga taaagaaaaa
     3721 gacggtctgc taaaagataa tctgatgtta gaacagcttt accagaaaaa caaagactac
     3781 tttgatgcac tgaatattta tatcgctgct ggagaattga aaatggaaga acttcaaaca
     3841 acgatcattc cagaagcgat gaaaagagca gaagaaactg gcgatcagat ggatgttcag
     3901 attgccaatg attacacgca attcctagat cgtctggata aacgtacgca tgatcttcgc
     3961 ttagctagac agatcacgat ccagcaagca ccacaaatcc gtttgatcca aaacacaaat
     4021 caggccttgg ctgaaaagat ccaagcatct atcgctacag ccattccgct gtggaaaaac
     4081 caagtcgtga tcgcattgac cttgcttcgt caaaaagatg cagtaactgc tcaacgccaa
     4141 gtctctgaaa caacaaacga cttattgaag aaaaattcag aaatgctgaa aatctcagcc
     4201 atcgaaacag cgaaagaaaa tgaacgtgga atcgtggata tcgagaccct tcaaaccact
     4261 caaaatgatt tgatcgaaac aatccaagag acattgcgta tccaaaaaga aggaaaagaa
     4321 aaacgtcgtc atgcagaagt agagcttgga cacatggaag aagatctgaa acaaaaactg
     4381 ttggatttaa cccaatagat cacctttcgc ttgttactaa aacaaacgcc caacaacagt
     4441 ttattacaac tgttgatggg cgtttttctt atctttcttc taacttctcc attggagtct
     4501 atttctcatt catcttgctg tgtccatagc catagccaaa ataaatgatc agtccaataa
     4561 ccattacgat accgaacacg atccatgtca cagtttgcag acgaagcatc aatacaatac
     4621 aagtcaagat ggagacgatt ggcaatagcg gaacgaatgg cactttgaat tctccagctt
     4681 ttggtttgcc aaacattttt ctcaacttca gtaagcctag actaacaagc atcaaatagg
     4741 cgagcgccat gatattcgtt agttccgcta atacattcaa tgggaaaaat ccggcaaaga
     4801 atacagctgc cacaccagcg gcataagtcg cttttttcgg tgtacgatgc ttttgatcga
     4861 tttctgtcat aaacttagga agtaagccat ccttgctgac agcaaacagt aaacgagcaa
     4921 gtgaatacat catcgagatc gttaccgtca gtagcgttaa aattgcccct acagcgatca
     4981 ccgcgcccac tgttccatgt cctacatacc tcattgcaaa agccactggg tcattgactc
     5041 ctagttcagt atacggaacg attcctgtca agatcagcgt cacgatgatg taaaggacag
     5101 tagcaatcag tatcgaacca atgattcctc ttggcacatc tttttgcggg tttttgactt
     5161 cctcggctgc cattgcaacg gcatcaaatc ccaagaaagc aaaaaacaca agagctgcac
     5221 catctagcac accggaaaaa ccaaaaggca tgaatggctg ccagttatca ggtttaacga
     5281 aaaatattcc tacaatgata aacaacgcga tgataccaaa tttgacatat accatggcat
     5341 tattcaaacg taatgctttc ttggcttcaa gcgacacgat gtaagtgacg aatatcacta
     5401 cgagcaccgc aatcaagtca atgtatgtac cattttccgg attgtacccg ccagttaatg
     5461 cttgtggaag ttcggcccct aaacttttca aaaaaccttg catatagcca gaccatccgg
     5521 aagcaacaga ggaaaccgct agtaaaaatt ccccgatcaa caaccaccct gtcagccagg
     5581 cagcaaattc accaaataca acatacagat aggcatatgg cccaccaatc actggcactc
     5641 gcgaagcaaa ttctgcgaaa cttagtccag acaaaatgac aacgattgca gctaagacaa
     5701 atgaaagtga cagtgctgga ccagcatttt gattggccgc aacccctgtc accacaaata
     5761 ttcccgtacc gatgatcgca ccaattccaa gcatgatcaa atcagacgtt tttagttctt
     5821 ttttcatctc actcgtatga ttgagattga tctctttctt acgaaacaaa tccattgaag
     5881 ttcctcccca ttttaggttg gaaaacagcg attaaactgt tttcatatac cctaaaagga
     5941 taccacctaa aatgactaaa aggcaaccaa caataacata aatgagctct tttttcgttt
     6001 ttctttcgcc aaggatgaaa atcccaccta aagtagagat aatgatcccc atttgtgaaa
     6061 gtgagaagcc aacagctaaa ccaagtgatt tgactgttag aagcatgcag atgtttccta
     6121 agccccataa taatccacag atcatgtttt tccacacaaa ctgatcgact ttaactttac
     6181 ggaaagcaaa gaagcttgcg cctaagatca ttccgatact ttgaggtaag ataattgaca
     6241 tcgcatccaa attcgcccag ttgacgatga tggtatatac accataacca atcgtagaga
     6301 agatcaatgc tctaaaaccc ttaccaaaat caagcatttt attatctgta tctatttttc
     6361 cttcgtcatc ttgtcgggca gtcaaatacg caccgcttac aagaagagct aaagcaataa
     6421 taccgtatac ataatctttc gtttgtgtcc attcatggaa gaaaacggct cccgcaatcg
     6481 tattcaaaat taattgaaaa ccagtagaca atggtagccc tacagaaaca cccatgtact
     6541 tcattccatg gaactgtcca ttttgtccaa cactccaagc taagccggat aaaaatccga
     6601 tcaagaaaat ccaaggggtg attacaggac taacaacgaa aaagacaact aaagaaaaca
     6661 gaaatgcacc gatcgtcatt cctagtgttt gttgattggc atcgccacca attttaccac
     6721 ttactaaacc aatactaccc catgccaaca tagggactaa tgctattaaa atttccataa
     6781 ccaatctcct tttatttcac atgataagaa acttatcagc gaaaacgtta acaacacaag
     6841 cacttttttt cgtttctaac gattttctaa cgtttttcat tataaaaatt taagattggg
     6901 aaaaataaag tcattgttgt cccttttcct tcagttgatt cagccgtcaa atggatacct
     6961 aatttttcgg ccagattttt tgctaaataa agtcctaatc ctgttgaatg tgttttgcta
     7021 agtcgaccat ttctcccagt aaatccctta tcaaagatcc gtttcaagtc ttccgctggg
     7081 ataccgatcc ctgaatcttt aactgaaagg ctgactcctt gcgcttcctt ttctatgctc
     7141 acaatgatgt ttccgtgatc cggcgtatat ttgatggcat tactaagcaa ttggttgaag
     7201 ataaaagcga tccatttagc atcggtcaaa acagtatgat cctcaccaac gatctcgtag
     7261 cgaatatttt tttggataaa ataatttgcc tgactgcgta tcacaggctg aatgattcct
     7321 tttagtccat attcttggat gaggtagtcc cgcgagaaac tgtcgagtct tgcataatac
     7381 aaaacttgtt ctacgtattc atcgattttt gataattcat tttctagtaa tgtatacttt
     7441 tgatcgtcaa tatcgaactc gatcgaacga actagcaatt tagaagcgga aagtggcact
     7501 ttgatttcat ggacccatga gtcaatataa tctttttggt cttgttgatt actgattgct
     7561 gattgcatta cttcttggtg ttcacggatc agctgattga tatattcttc taccagtttt
     7621 tcttcttctg tcctcccacc actcaaataa ttttgcaaag gggaagtttt ttgtatgtcg
     7681 gctaattttc cccaccatct tctcttgctc aagtaaccac ccagcagata aaaaatcaga
     7741 aagacgccct caatcaaagc taaataagca attgtgctca aatcaacttt gatgtttggc
     7801 gccagccaca tcacaaagat cgtcaatgca ataaaaaaca gccatcccaa gatcaatgtc
     7861 cactgatctt tcaaatattt aaaaaaattc atcttaaccg ctcatccttt cagggaacga
     7921 tatatccctg tcctactttt gtttcgatat atccttggat acctgcctgt tcgatttttc
     7981 ggcgtaaccg attgatgttg actgtcaatg tattgtcatc tacaaatcgt tcgtcatccc
     8041 acagtgctcg cagcaatttt tcccgactta agatctttcc attgtgcttc atcaaaataa
     8101 ataatagacg atattcattt ttgctcaaat caatgatctc tccgttgatt tccatacttc
     8161 cgttgtcaac attcaacgta atgccattgt ggctcatcat ttcactagat aattctgtgt
     8221 agttgtaaga acggcgaagt aatgcattga tttttgcaat cagtacatcg atttggaaat
     8281 gttttgtcac aaaatcatct gcccccatgt tcatagccat gatcatatcc atattcgtat
     8341 tcctgctgga gataaaaatg atcggtactt tcgatacttc acgtattttt tgacaccaat
     8401 aatagccatc caaaacggga agattaatat ctagtaatac aagctgtggt tcttctcttt
     8461 caaagtctga gaacacttga ttgaaatcgg tcgttccaaa ggtttcaaat tgccatttct
     8521 caagttcctc catgatcaat tgtcgaatga tctcttcgtc ttcgactacc atgatttttg
     8581 ccataaaaaa tccctccatc tttcttcttg ctactacaat atcaaagaag cccttgtgaa
     8641 acaagttggc ttacctggaa aatgtcattt tagcaaacgt taaaaaaaac tggaaatatg
     8701 cgatttaatc gcgtttgtct cttccaagca gcttaaattt attatgttat tcttaataat
     8761 tggtctatta tctgttattt ttctttcgtt gtttttgtct tttaaatttt cgataacacc
     8821 aataaccgaa aagcgttagc actagcaata tacctagaag gagtataagt aatcgataaa
     8881 gatggtattt ttttgtctgt tctatctttg aagagacttc ttctgtaaag ggaactcgtc
     8941 ttcctgtcac tagtaatcgg tgagtgttca cggtgtaagg tgtacaagtg ataagtgtga
     9001 ctagatcctg cttttctcta attgctaatg tatttgtttt tgttggaagg acaacaattt
     9061 tctcaacgac ttcataagcg agcatccgac catacacttc aagatagaat cggtcatctt
     9121 gctccatttc ttcaagatcc gtaaagagtt ttttctcagc aataccgcta tgggcggtaa
     9181 tcacggaatg tgtactctct cctccagttg gaaaagaggt gccttgcagg actgtcgctc
     9241 ctctatctaa caaaagatca ttcgtttcat caaataaagg caagctaaca ttgattttcg
     9301 gaatatagat ggctccgatc atatgttgtt cgtaatattc ttttcctgga ttttggacat
     9361 ttctaaccgc ttgctcaaaa ggatctttga cttgtcccat tcctgggatt actgatgtat
     9421 gagcaagcgt ctgatttttc ttttcatgca ctgctaattg ttgctcatgt ttttcttctt
     9481 tatcgcgttc aagctgcttt tgataattct cgatccttct ttgatccaaa taattgctga
     9541 gagcatctgc taaaaatgga tatgtaaaaa ctgcagctcc ggcaagaaac atcactgcca
     9601 taaataattg taataaaaga ctgatttggg tttgaggtct attacctatt ttctttttca
     9661 ttttcttcac cttcttattt aaaaaaactg cagcaacctt tataaaaccg attgctgcag
     9721 cttatttttg ttgcttatac ttcagacttc attttagatt tacggtacca gctgtacgct
     9781 ccaatcatca aacttattcc gattagtaag aatgccaaga gcccgttccc accagtagca
     9841 ggaagtgacc cgcgtttcat attcggtact ctttctggcc ctggattttc tactcctacg
     9901 atctgaattt cttcattata gcttccataa gagatgataa attcagtaaa ggctgcctca
     9961 cctgttggaa gtacataacc ttctggcgcc atggtctctt tcatttgata ggttccataa
    10021 tctaaccctt gaacctcaaa gtatccattt ccatctgcga caaattctgt tgcttgtccg
    10081 cttgttgccc actcaattct atctgctcct gattcataaa cagctaaagg cgactcccct
    10141 ttataaaata ttgcgtattc agaacggtct tcattccaca attcgaatct tgcatcttta
    10201 agcaattcac tgcttgatgc atcatatttt tcaaactttc ttccacctgt tgtgactttc
    10261 tctggcggct ctactccttc ttcataatca aattcatcat ttcgtccgat ttcaataacc
    10321 gctgtgttgt tatggaaatc atccgcttct agttctgtgg aaaccttcaa attatacgtg
    10381 atcgttaaat ctcctccacg tgcatctgct aatctaccta aagtatctac tttggctact
    10441 ccattttctt ctgtcaaaat cgtcacgaca aacccgttgc ttactacttc tactatataa
    10501 tcttgtcctt ttgttaaaat cgtctcgccg gctcgcactt ctatttcctg atcataataa
    10561 gttagaccaa ctgtaggcgt atctgtgatt ttgtaatttt tgataacagc gcttccgttt
    10621 tcaaggacag aatcgatgaa ataaggaata ggtgctgtga tcgtgtaaga agccgttttt
    10681 cctctttcta gatcaatata agaaatcgtt tcttgttctc cagcttcatt aataatcgtt
    10741 acttgtctaa ggtcgctttc ctctacattc atctgtttat ctgcttctct gatttcgttt
    10801 ttcggataca aatgcacatc tgtattgtat gtgtctttcc atgtagaacc atcccacacg
    10861 ccatccacca tatcaggcat catcactggc atcataacaa ccattggaga cgcgattctg
    10921 gaaatatgag ctggcgaata agtttctaag aaaagataga ctttatctct agaagaaggt
    10981 gcttcttccg tcacttggac ggtagagaat accgcttctc cagcttcatt cgttgtttgt
    11041 ttgtcaatac ttgtcagtcg atattttaaa aaccaacttt ctgtgtgccg tttttgaatc
    11101 cattcaatcg cttcggcaga attaagaccg tcttctcgtt ccggtgttaa aggattatct
    11161 cgaatcaaat cgtaatatac ttccgttacg tcaaacaaat caaaatcgac tcctggaata
    11221 ttttcttcac caaaatccgt agaaaccaat ccgctattcg gtcgttcttc cggaacagat
    11281 gagaaacctt ttttgtgtaa tgtaatcgtc acttcacttg gtttttgtgc gacttcttcg
    11341 gctttgacta gttttccgtt cccgaaaatc cctattcccg cagtaacgag taacagtaca
    11401 ctcattcccg tgacaatcat ttttttattc atttttttct ccccgttctt tttttattaa
    11461 taaaacagca cctaacaata aaagaattcc tagtaaagaa atcaacgttc ttgcttcatt
    11521 cgtttgcggg aatacggacc attttcctga ttgacctgga ttgcttggac cactcggctc
    11581 tttcggtttg tccgttgttt cttttacggt ctcgttataa acacgtggtg ttgcggattc
    11641 ataggcagag actggaactt tcccggattc taattcagcc atctcctgtc cgttaactac
    11701 tacatattgt ggttggcctt ttgcatccac ccaaaattca gggatgatga cctcgattct
    11761 cttacttgct tcatccatct cgtaaccttc cacacctttt aattcttcaa aataataggt
    11821 gccagaaggc aagaaacgtt gtcctgtcgt aacaaggccc tctttatccg aaatgaattt
    11881 gcttacattt ttgtcattca aaggatcatt gtctgctggt ttgatccatt gatttttcaa
    11941 gtcattagca ggagccatat ctaaataata tttcccccct tctatcatct gatatagagc
    12001 aaaaacagca ccttctaatg gttttccttt ttcagttgtt cctctttgac gtccatactt
    12061 gaagaagtat ggatctctta gataaccgac atttttagga tagatatgaa tctcttgcag
    12121 ttcctcggtt gattctgtcg ggtgataaat tggcaaaata gcagcaattg ggatagctgt
    12181 ctgatcgata tcaacatcca atccaacttc tggatcaatc gattcttcga aaatcaaata
    12241 aactgcatcc cgatcttggt ttttcttagg tgctttgata cgtgcaatcc cgtctcctaa
    12301 tccgccaacc gtatctttcc cagttgttac cgaagtccct atttggacaa gcctttcttc
    12361 tgtgattagc tgacggatat tgtttctgtt cgtttcagcg atttgctgga cgaattcttt
    12421 aggagtcatg ttttcagcag cttttttttc atgataataa tcggtcatgt cgtaaatggc
    12481 aaatgtcgct ccatttagtg gcaccgtctg tgaaatgata tctgctgttt cccctgcttc
    12541 ctcagagata gccaagccat cattttcatg gtattcaaat tgatcgttat aatcgatatc
    12601 tcgaatcatc cgtttatgca acacgaattc cacgacatct tcccctgatt cttctgccga
    12661 catcgcatgt ggaagaagga ctgctgcaag cggtagtaaa acaagtaatt gaagcatcaa
    12721 acacaacagc tttttcatta ccttgctccc tcctttttag attgtctgac aaacaaacca
    12781 taggctgctc ctgcaagcgc catgaagaat gcagcaagca ggatatagaa taatcgtccg
    12841 gatcctcctg tagcaggcaa cggattttca ggatcgttcc cgacactaag ctgtaatctg
    12901 attcgatttg tctccaaaag ttcatattct aactcttgct gtagatcagg cgtctctgct
    12961 tcttcgtttg gataatacga aatctcaact tttagtgtac ccgcttcttt tcctggaact
    13021 acccgatcat tttctatgtc aggttcttct cgataactaa tttctaaaat aaagtaacct
    13081 ggaagttttc tatagctgtc aggtgacttc acttccttga tccgatagac cccttccaac
    13141 aagtctgaag cattgaatag ggaagaatcg tttggatctg gtgccaagcc tccctcgatg
    13201 atttcatact ctcctgcact attttgtttc tcaattgaaa attctgcgcc ttcaagacgt
    13261 cggctattca tactgtcaat tttcaaaagt tccaaatcga ttggcgctag ttcatcttct
    13321 aaggttactt gtagtacacc atcgatgatt tcatagcctt ccggcaaggg agcagtttcg
    13381 tcttcgatga ccgtatcttc aatgataaac ttaccttctt cattcagtgc aaactggaac
    13441 acttgatcag atagccgata cccatttggt gcgacagatt ccgagacctc atattcacca
    13501 atagacagat gcagaggttg gtcgtaattt aaatcgacat acttttctac tcctacggca
    13561 cgagaataga agtatccgat cccaggcaaa cgtttcgatt cttctggttc taacggatta
    13621 ccagtgtatg ctcgatacag cgggaaggaa gaatccccca ctttcctgat atcgaaacct
    13681 gcaccatcta atgtatggcc ttcatggtct aattttctaa ctcggatctc ccgataatca
    13741 aacgtattgt aagcagtgaa tgctacccat aaacgatcct cttcatctgt tgtatacgtg
    13801 agatattgcc aattttcttc acctgtttgc ttgagttgga tagtccctat agcattatcg
    13861 taaagtcgca agatccatgt gtcattttca tcaggttctt tgaaaccttc tgggttctgg
    13921 ctctcaatga ttttgtattc tgcatagttg gtagtttctt cactatcctt ataaacaaat
    13981 gaatctggct ctccgattgg atgaataact ttctcaccat ccacgattac atatttcccg
    14041 tcttcatcta ctgaatagaa cgtgagtctc cctttctctt cctctacttc gttcacacca
    14101 gaaagccctt tcgctactgg atatccgatt tccagtccgt taccatctgt ttcataaagt
    14161 tcaaactctg cgccattgat tggcatatta ctgccttcaa ttagtttatt gatattcatg
    14221 tggattggct tgaattcatt tcgcatttcc agtttgacat acaactcatc atactctgtg
    14281 ccttctatgt taggctcttg tacaacagaa agcttgtgat attctccttc ataataggaa
    14341 ttcggatctt taccttccgg tccgccgact ctcgaaccag atgtatattc atctacaagc
    14401 tcgtagtgac ctaacatctc atcttcgttg gctgcatatt ttttgatttg gaattgatac
    14461 gtctcatcta ctaactgata acgtggtggt ccagcgatct ctctgatcca aaatatgtcg
    14521 ctgactgctc cagtttcatc tcctaaaatc ggcatcttcg tttcaaagat cgctattccg
    14581 tccgtggtag ttttacttgt ttcgattggt tcggcttccc cactctctgg attttggtca
    14641 tataatgcaa attcgactcc agccaacggc tcgccatttt gacccttctt gctgacttgg
    14701 aaattacggc ctttatgttc gtaacgaatg gaagaagatg ggaaatacat ccgtccagta
    14761 gtgatatcat tctcataatc ctccaggttc tgaatatagg ccatattgtt cactgtgcaa
    14821 aaatttccgt cttgagctcc cgcatcaagt tttacttggt acttgatcct gaccatttcc
    14881 ccttcaccta aagaaatcgg agaaaattta taagcttggt tttcccgtcc gacatacgtt
    14941 gtcggagtcg ccactacttc tggtggggct tgtccatgac gttcttcatc ccatatttct
    15001 ggctcttggt acttcatgcc gttttctcct ggatctagat ggtaaaactg aatatctgcc
    15061 gatctgccag ctccaaatgg tgagacatta aaaccagttt catcataaag tgtggcttgc
    15121 tggatcgttc tcttgaaggt agaagagata tcctccaagg ctgtaacaat gtcattcttg
    15181 ttgttcgcat tgtaatatcg cggtgttacg ccatcttctt ctaaatcagc aatatttttc
    15241 aatacatttc gtccaatagc atctagtcgt tgtctcccgc taacgtttcc atcgattcct
    15301 aaaccaatgg agaagatact tggcgggttc tcttgatact tgtttttcag ccaatgatga
    15361 taagcaactg tgttgatcgc agatgacttt tcttgcgaag taggactgcc attcgtattg
    15421 ttattagaag aaccatcacc aacccagtga gtcgctttat tatctccttc ttttacctct
    15481 ccataacgat agtgtacatt tacttcatct tctggacggt taaagtcaga tgcataggta
    15541 actggatgag taaaccctgc attagaagta ttcccagaaa actcctcaaa atttctgaac
    15601 aaaacgtcat tatcttcagc aatcttatct tccatcacag tccagttgct ccaagctcca
    15661 ttagtgctcg tacgataact tgactgaatc ggtgcataac tgaaggtcgg tgtcccatct
    15721 cccacaacga ttaggatttt ttctggattt ctattttctc cgccattgtc tttatacaat
    15781 gtttcatagc catttttaag ccctagtgta agcggagttc ctccgattgg tgtacgattt
    15841 aatgctgtat tctcacttcc cgtcaaacga ttgatatcat ttgtcaatgg aaagatgtcc
    15901 gaactgattt gctcatggtt attgatagct gtactattat ggtaaaaatt caccataccg
    15961 attcgaatat ccagactcgg gtcagagagc agatctttac tcatttcgtt tactgcctca
    16021 atcaacgcag catttttagt ttgactattg gtcccagcgg ttaattcact catactcgct
    16081 gacttatcta gaacaaaaac gatatcgatt ggactgattt cctttttctc gcccccgatg
    16141 acatctagat agacattgta aagggttgtt gcatcttcag gatgtagatc agatgctgtg
    16201 ctgcttggca gaacaggttc tacccatttt ttgatgtacg ccccgttata atccaagtaa
    16261 ctgttttcaa aattccgttc atttccccaa agctgaacgg tttgagctgg aacgtttcta
    16321 tcttcttcgc ctgcagttga tccattatta gtagcatagt cgtaatttcg ccagttcgct
    16381 cccccgtttg tataatcact atcatcgatc aagttctctc cgtcatatgg cgagcctttg
    16441 tctccttgat agcgttcatt gctttgtgtc caatttccta acacttttgt atagtcattc
    16501 gttgtaaact tcatctattc tttcatcaac caccacttga agaggtagtt acccttcgtt
    16561 cacatcgggg atcttttctg tttcaaaaat caatgtgccg gtttctgctt cttttgaata
    16621 aacttttccg acataccatt cttgaccatc gatcgtctcg acttttaggt cttctccatc
    16681 ttcatcatag gaggatagac ctgttccacg aacgtttttt acggtaccaa tcccggcggc
    16741 tgccgcatcg attgctaaac gtattctacc ttcattggca ctttcctttt tttcaaaagc
    16801 cagatgccat tcaagggttt cttctttttc aatatacttc ccttcaattt ttgcatagtc
    16861 ctcatctaag aatacctcat cctcctttgt ttctgttgac tcagcagcta catccagggt
    16921 cgtcatatga tagactgact ggaaatatgg tccaaatagc aaactgacag ttactaactg
    16981 caaaagcttc aattttaacc ctttcacatc cttatactcc tttcaaaaat atttgtcttc
    17041 tttcacaaca atcaacaaaa caatgcccat ctaattataa tttaatttaa ttttaatttt
    17101 aaatcaaatg atatcctttt tagttaaaaa caccaattat acaacattta ttattaaaat
    17161 taaattataa aattttataa cgttctcatt taacaaaact taagttatta agacaaatga
    17221 ataaacaact acgctttcct cgaaacaaaa aaataaagca caaatgcgca tttacgcact
    17281 catgctttat tcgtacaatt aattcagata acggcagcgc ataggatgcc aataacactt
    17341 cctaaaatag ctcctcctaa aatgtcagta ggataatgta aaagtaaaaa aatcctcgaa
    17401 taagcgatca aacaagcaaa taaaaaggct aatattcctg caactggact taattcgaac
    17461 aacgcagcta ctgataaaaa ggctagcgct gtatgtcctg aaggaaaaga agaagtcttc
    17521 ggtttacttg tgtaaaggac aagccgacga tttttttcat aaggtctttt ccgtcttgtc
    17581 gtttttttga taaaaagcaa accaacaaaa aaatagatca aactgccgat aagaatggct
    17641 agaccatatt ctctagtatc aggtatcaag actaaaacag cagcaaggag caaccaaata
    17701 aaaccaccat tagagatatt actgataaat ttcaaaaaat tgatttttcg actatccgtt
    17761 ttccgttgct tggcgaactt gttgataata ctatcatcga ttttagcaat caaacgcaac
    17821 atgttattcc tcctttttaa gaaccttttc ttgcgtacga tggataaaaa gcaggacttg
    17881 aaaataaaac aagaaactga aacctaataa agcaaaatag attggtcctg caaaccagaa
    17941 tgcgacaaga aggatcaacg tgcctgccaa atagaaaagc gcgcttttga aatcattaaa
    18001 aaacaagaaa aatgcagtaa tccaagaagc ttctttttgt tccagttgaa gcaagatttt
    18061 gtaactaaag agaagcgcgc ttaatagaag caaaaacaac gtagtataca tcaatgtata
    18121 gccaattccc ccaccccaat agcggaaaat ctgataatcg atcaatagaa gaagaaaata
    18181 aacacttgct aaactatctt gctttccata aagagagaag ttctcacgaa actttttcca
    18241 gaatagtgaa ccgagctttt tctcttcatt ttttttgtaa tgataaatca tatacagaac
    18301 tgttcgactt gcagttccta atgtgaaaac aggcaagacg cacaggaacc atagtagctg
    18361 taaatggata agacgtggaa tccattcaat gatttctaag aaataatcat tggctttttt
    18421 accaatcgat cgattcatca tccttttgaa cctcctgtca aatccatccc ttcaatgaaa
    18481 taacgttgtg caaaaaagaa cagcaaaatc gtcgggatca agacaagtgt tgctcctgcc
    18541 atcagataat tccattgtgt cgttgcttgg ttctgaaaaa cttgcaagcc aatctgtaac
    18601 gtgtattttt cttgatcttg gatatataac agaggaccta agaaatcatt ccaagctgcg
    18661 ttgaaggaat tgatcgcaat ggtaatcaag gcaggtttcg tcaatggaag catcaaatga
    18721 ctccaaatat acaaatgatt cgctccgtcg atttcagcag cttcgatcaa ttcattattg
    18781 atcgataagt aaaattgacg catcagaaat atattgaacg cattgccgaa gaacgatgga
    18841 acgatcaggg gcaaataagt acctacccag ccgatcttgg agaataatac atattgaggg
    18901 atcatcgtta caaaccccgg gatcatcatc gtagaaagaa ccaaagcaaa catcagcttt
    18961 ttcccaggaa aatcaagctt cgcaaatcca taagcaataa aagaatttga aaggacatta
    19021 ccgatcacta ctagcacagt gatgaataat gtatttcttg catagcgcca aaaaggaaag
    19081 gcggcaatcg tatccagata gttttcccat acgatttttt ctgggaaaaa ggtcggagga
    19141 taacgaacga tttcttccat tggtttcaat gaagtaaaca ccatccaaag caatggaatg
    19201 atcaaaatga ttcctattac tgtcatgagg acataactta gtattttttt tgtttgccga
    19261 ttcacactgg tctttttgaa ataatccatc atttgccgtc acctccagaa taatagaccc
    19321 acttaggtgc aagcttcaaa ttgacaaacg tcagaaccaa cacgatcaga aacaggatca
    19381 ttgacatagc catcgcataa cccatatcaa aagctacgaa cgccttgttc cacatatgca
    19441 gattataaaa aagcaaagaa tttgcaggtc cgcctgttcc atttttcgtc atgacataag
    19501 cttcttgaaa aatttggaaa gaaccgatcg tagaggtgat cacatcgtaa aaaataatag
    19561 gagtgattaa tggcaaagta atacttttga actttcccca aacgcctgct ccatcgattt
    19621 ctgctgattc atataaagat ctaggaacat tttgtaatgt agctaagtaa agtagcattg
    19681 ctcctccaga actccatatt ttcatcaaaa tcaacgcagg tttcgtccat tgtggatcaa
    19741 acagccaagc tggtccaggg atatggaacc agcccagaaa tgtgttgatc aatcctgcag
    19801 atggagaaag cagctgcatc cataagatgt agactgctac tcctgataag actgccggta
    19861 aatagaaagc tgtacggaat acactgattc ctttgatttt ttgattgagt actgtggcta
    19921 aaaacacact gaaaatcgtc gtcagcggta cattgaagat gacgaaataa acggtgttgt
    19981 ataacgattt ccaaaataaa tcatcgtgaa cgaacattct tttgaaattt gctagaccaa
    20041 tgaaattcat cttggatgtc aaattataat cggtgaaact accaataact gaaaaaagca
    20101 taggccctag cataaatacc aagaacccga taataaaagg cgagataaat aaataggccc
    20161 atcttgcttc ttttttcttt cggatagata aacgcttcaa attttcttgc ctcctctgta
    20221 cctagacagt caaaaatgga ttattgattt tgcttgacta agttttctac tgctttttgt
    20281 gcatctgcca atccttcttt tggtgatttt gttcctaaaa gaatctggtc aatctgttca
    20341 ttcactaatg tcgtgtaatc cggagcatct aaaggaactg gtgtgatcac cgtattttta
    20401 aagttttcgt ccgccatttg atagatcatt tttcctttat ccgttaggtt gtcattattc
    20461 actaaattct cattggctgt cttgctcgcc atgatatcaa aacttttttc gccgaatttt
    20521 tcttgtactt ctggagaaga aagatatttg atgaattcat aagaggcctc aggatctttt
    20581 gctccgtatg gtacttccaa gacgaatccg ccaccccacg accaatggcc tgaaccttct
    20641 tctttttctg ggagctgcat cacgccaaaa tcaaaatcat ccggtgcatt ttccatcaaa
    20701 ctagagtaat aattgatatt ttgggcacgc atggctacta atccggaaat aaatggatca
    20761 gatacaccag acccaaattc ggcttctaat ttattgatcg tatttttccc ataataatct
    20821 tgccagtctt ggatccactc caaggcttcc actttatttt tagtatcaat cttgactttt
    20881 tcatctttgt caaaccaact agtcccgtta tctgcgttta gcgcccatac atcagcccct
    20941 aaattccata atggataaaa accgatgcgt gtaaattctt ttccttcttt tttatctagt
    21001 ttacgtgctg tttcttctaa ttcatcccaa gttgtcggta gatcttgttc actgattcca
    21061 gcttctttaa ataacttttt attataaaaa atgacttgtg tatctgtatt gaatgggatc
    21121 gcataaggat caccttcata ttctactgta tcccaaagct gcggataaaa atccttagaa
    21181 gtgtcttttt ctacatattt ggagagatta gttgcttgct gtgcttctgc tctttgggca
    21241 acggaattta tgtcctgtac aataacatcg ggtgcatttc cagctgttat tgcagataaa
    21301 gatttagtcc agatgtcacc ccatggctga tacttataat ttacatggat ctcgttttgt
    21361 gattgattga aatcctcaat gatttcttcg atagcttctt ttcgtgcacc ggatccccaa
    21421 aaggaccaga aatcaatatt ttttcttcca tcacttgttt tttcagaaga atttgctgca
    21481 cttgaactgg catcgcttgc attacttgat ccgcatcctc ctaataaaaa caatgctgaa
    21541 cttgccagca accctgtaaa caataatttt ttcatatttt acctccaaat tttaatgtaa
    21601 ccgatgccgg ttttaatata acgatatcat tttgttctga aaataacaat ttataaggta
    21661 tgattctaaa aaaattatta atgataccaa tactttatat aattcgcgag cgctttcttt
    21721 gaatttttta atctttcgta gcagaatgag aatgtattaa aaatagatta ggaggaattt
    21781 tgatgtttag acaccagaaa gaattgcagt ttgaagcaaa accagatcgt ccgaatccat
    21841 taatcgcgaa atatctgcag gagctgatcg gcggccaata tggcgagatg agtgtagcta
    21901 tgcagtacct gttccaaggc tggagctgcc gaggagaaga aaaatataaa gatatgctga
    21961 tggacattgc aacagaagaa atcggccacg tggaaatgtt ggcgacaatg gttgctcgtt
    22021 tattagaggg tgcgcctgtt gaagatcaag aagcagcgat gcaaaaagat ccaacaatcg
    22081 gtgccattat ctctggaatg aatccacagc acgcgatcgt agctggctta ggacctcgtc
    22141 ctgctgacag cgtgggcaat ccatggagcg gtgcctatat cattgcgagt ggcaacttgt
    22201 tagcagactt tagagctaat ttaaatgccg aatcacaagg aagacttcaa gtagcacgta
    22261 tttacgaaat gatcgatgat cgtggcgtaa aagatatgct gtctgttctc cttgcaagag
    22321 atagctatca tcaaaatcta tgggaatgtg cagtgaaaga attagaagaa aaagaaggtt
    22381 caatcttagt cccaagtact ttcccgcgtg acaaagaacg aaaagacatt gcttatgact
    22441 tctataactt ctcagaggga gaccaaagta gtgaaggttc ttgggctagt ggcaaagcac
    22501 tggatggcga aggcgaatat caatggttga aagaacctaa gatcgaaggc gctgcaccaa
    22561 aattagaacc agtaacacca aaaatgtatg ggacacctcc attcaagaaa taaatatcta
    22621 cccgtagaaa cacccgtttt ttgttccaca cgctttgtga gcaaaactga ctaaagtcat
    22681 taatcataac cacccttcaa aagggtggtt ttctctagag ctataagctc aaatcaccgg
    22741 ccagcgccta aagacgctgg ctttcatatt ttatgttcaa gccatattgc ctttgccagt
    22801 tttgcccacc tcttaaaagg tttatgtact actccttagc ccttaaaagg atcgtcttac
    22861 tctcgtacac tcagcttatc taacgcacta tcatgttttt cttgttcttg aatgtacttt
    22921 ttgatggtcg cttcattttg tcctactttt gtcacatagt atccttccac ccaaaagtgt
    22981 cgatacccaa atttatattt tagatttgca caatctcttg atgatctcct gaatattggc
    23041 tcgatattga ttataaatta tttttcttct atactttggt gtgaatacaa tgtggtattt
    23101 acacatctac tttgtacggg ctaaactatt tccctttatt gctccacccg catagcaagt
    23161 ggttttgtat ttcaatcgct cggcgcgctc aattaggtct aaagacaata aatctaaaaa
    23221 cccgctgctt cactgacaga aacaacgggt tttcagaaaa aaatgaaatg aaaaagatct
    23281 cttacggcaa tctattttat ttttttgatt ttttataaat ggaaaatgct tcaagaagtc
    23341 caagaaataa agccgtataa aaacaagaac ctatgaaatc tttatttatt agatattcaa
    23401 cagaaatccc gataacacta gccaacaata tatttatcag aagtgtaaaa taaggatgct
    23461 ttttatagaa tagaactagt gttgttttca tagactctcc tttcttcata tttccaatta
    23521 atcacaaatt cacggcatta ctagaaaaat taaaggggcg cagaagtgga aaaatactct
    23581 tcgactttcc ctacaaacca ggccatcttg tgtttaataa ctataccaaa tctaaggtac
    23641 ctctaaatct aaattccatt tgtggcggtg acctccaatt tttaactctt ctatctccca
    23701 tccagtatac ataaccttga tacttcgacc acttatatat cgaacacaca aatacctatc
    23761 cattgcagga gtactaccta acttagttgc tacttgcatc taaataacag ctgaaaagat
    23821 aggacttact cttagtaaag tatctgaact attaattttt aaactatcat ttttatcctc
    23881 aactacatgc acatctgttg caacttcact ttctgtctca tccagataaa tgattaagga
    23941 atcgggaatt gcaatatctt cagctgacgc tggaatagtt gagctaaatt agtcatcata
    24001 tctttattat aaaataacca acagcaaaaa atacaatgaa aaacaaactt ggaactaatg
    24061 tatcattaat aaatgttgaa aaattcagca actcattact gtatgatttt agcagcccaa
    24121 ccaacatata ccctataaaa gctaataaga tggttaggaa tcctttagaa tttttactca
    24181 ttcatagaca cttccttaaa taacttttat tatagaaggt attgtaaata ataatattga
    24241 caaaaagtga ctcaaaataa aaaaaaactt acattatact ggaatgaact gtattgatag
    24301 tgtacctaaa agctctactg acataagatc gtgtaatcgg taaagtaatt ttattaatta
    24361 tacgggatgt aatcaattag agaataaact aatcataaaa ggtactttta cattcataat
    24421 tgtcgctttg agattctact ttggttcact atctgaccca attgcacatt ttcgatcatt
    24481 acaagtattg gtgtcgttag aacaactaaa taaaaactag cggtagattt ttcagaatct
    24541 atgctaattt ctgtatggat gttccagtga tggcaaattt ctcttttcat tgtttttctt
    24601 gtcgcatgat atgccttttt gtgttattca gttaaactat agaaattctt tcctagacag
    24661 atcttgatcc caaattttac gtttttgagc atcgatatag tttaaaatga cattactcat
    24721 atctaaagag tttagatttt ttaggactaa aactgtcagt tgttcttttt cttctttctg
    24781 ttaaagtatt gtcgcgactt ttcgtatacg ttaagatatg tcgtgaaaaa acaagttttg
    24841 tgacacaaaa aaacgaatga cttgaggaaa tcacctcaat cattcgttta aaatgctata
    24901 tattctttag tttaagatac ggaaattaat gccatcgacc acactagtag aatgaacgac
    24961 atttacttgg ccttttttta atttcgttat gtttagcata taaaaatatt atagagttta
    25021 cttacaatgc ttaaaacata tactgtataa tctgatcaat tttagccatg tcttgcaatg
    25081 gtaagttttc tatttttttg agttttcgtt ccttaaaatc aagagatttt aattgtgtaa
    25141 taattacttg tccttttata tctagctctt caggtaatga gtaacgtgtc ggtaaattct
    25201 ttatagtgga agtaattgga catatgaccg aaaacattgt tttacggtta aattcataac
    25261 gagagacgac tagtcctggt cgtctttttt gaatttcttt tcctacggag ggatcaaaat
    25321 caatccatac aatatcacct tttttaggaa tataaattct ttcttctgac actataaaat
    25381 ttctctccct tcaggagtta aatcttccca ttcgtctttc tcataatact cggcctcttc
    25441 cccaccacta aatggatctt caatttttgg aactaacgta attgttccat catcagaata
    25501 cacaacgata tattcttggt tttctgaagg tttctttccg ttatcagaag gtaaagttac
    25561 cactacagaa cttccttgta atctagattt tgtaacaagc attatcatca ctcccttaat
    25621 aaaagtatac tatataacac taagtaacgc aaaattcccc tttgtaaact atctagaaaa
    25681 ttgttttgtt ta
//
LOCUS       DACDIG010000037        25672 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.037, whole genome shotgun sequence.
ACCESSION   DACDIG010000037 DACDIG010000000
VERSION     DACDIG010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.037"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            172..516
                     /locus_tag="IV247_001592"
     CDS             172..516
                     /locus_tag="IV247_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAP8777666.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            517..936
                     /locus_tag="IV247_001593"
                     /pseudo
     CDS             517..936
                     /locus_tag="IV247_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(1028..1378)
                     /locus_tag="IV247_001594"
     CDS             complement(1028..1378)
                     /locus_tag="IV247_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777667.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1344..2675)
                     /locus_tag="IV247_001595"
     CDS             complement(1344..2675)
                     /locus_tag="IV247_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAP8777668.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2931..3497
                     /locus_tag="IV247_001596"
     CDS             2931..3497
                     /locus_tag="IV247_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8777669.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3642..3729
                     /locus_tag="IV247_001597"
     tRNA            3642..3729
                     /locus_tag="IV247_001597"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3674..3676,aa:Ser,seq:cga)
     gene            4063..5037
                     /locus_tag="IV247_001598"
     CDS             4063..5037
                     /locus_tag="IV247_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP8777670.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            5362..7257
                     /gene="asnB"
                     /locus_tag="IV247_001599"
     CDS             5362..7257
                     /gene="asnB"
                     /locus_tag="IV247_001599"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAP8777671.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEKWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(7452..7898)
                     /locus_tag="IV247_001600"
     CDS             complement(7452..7898)
                     /locus_tag="IV247_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP8777672.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8126..8275
                     /gene="rpmG"
                     /locus_tag="IV247_001601"
     CDS             8126..8275
                     /gene="rpmG"
                     /locus_tag="IV247_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP8777673.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            8363..8881
                     /locus_tag="IV247_001602"
     CDS             8363..8881
                     /locus_tag="IV247_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8777674.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(8958..9914)
                     /locus_tag="IV247_001603"
     CDS             complement(8958..9914)
                     /locus_tag="IV247_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8777675.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10080..10886
                     /locus_tag="IV247_001604"
     CDS             10080..10886
                     /locus_tag="IV247_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8777676.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            10883..11872
                     /locus_tag="IV247_001605"
     CDS             10883..11872
                     /locus_tag="IV247_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP8777677.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            11869..12873
                     /locus_tag="IV247_001606"
     CDS             11869..12873
                     /locus_tag="IV247_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP8777678.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13005..13547
                     /locus_tag="IV247_001607"
     CDS             13005..13547
                     /locus_tag="IV247_001607"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAP8777679.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            13567..14265
                     /locus_tag="IV247_001608"
     CDS             13567..14265
                     /locus_tag="IV247_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAP8777680.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            14286..14498
                     /locus_tag="IV247_001609"
     CDS             14286..14498
                     /locus_tag="IV247_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAP8777681.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            14498..15460
                     /locus_tag="IV247_001610"
     CDS             14498..15460
                     /locus_tag="IV247_001610"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAP8777682.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            15478..15873
                     /locus_tag="IV247_001611"
     CDS             15478..15873
                     /locus_tag="IV247_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP8777683.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16036..16401
                     /locus_tag="IV247_001612"
     CDS             16036..16401
                     /locus_tag="IV247_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAP8777684.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            16398..18209
                     /locus_tag="IV247_001613"
     CDS             16398..18209
                     /locus_tag="IV247_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAP8777685.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(18271..18438)
                     /locus_tag="IV247_001614"
     CDS             complement(18271..18438)
                     /locus_tag="IV247_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAP8777686.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            18686..19675
                     /gene="galE"
                     /locus_tag="IV247_001615"
     CDS             18686..19675
                     /gene="galE"
                     /locus_tag="IV247_001615"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAP8777687.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            19687..21171
                     /gene="galT"
                     /locus_tag="IV247_001616"
     CDS             19687..21171
                     /gene="galT"
                     /locus_tag="IV247_001616"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAP8777688.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            21195..22175
                     /locus_tag="IV247_001617"
     CDS             21195..22175
                     /locus_tag="IV247_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP8777689.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            22264..23088
                     /gene="tsaB"
                     /locus_tag="IV247_001618"
     CDS             22264..23088
                     /gene="tsaB"
                     /locus_tag="IV247_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAP8777690.1"
                     /translation="MYYCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDNIIVPMFDARRKNVYAGAY
                     RWKENRLETVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFSEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            23072..23614
                     /gene="rimI"
                     /locus_tag="IV247_001619"
     CDS             23072..23614
                     /gene="rimI"
                     /locus_tag="IV247_001619"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP8777691.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            23621..24085
                     /gene="rimI"
                     /locus_tag="IV247_001620"
     CDS             23621..24085
                     /gene="rimI"
                     /locus_tag="IV247_001620"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP8777692.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            24082..25098
                     /gene="tsaD"
                     /locus_tag="IV247_001621"
     CDS             24082..25098
                     /gene="tsaD"
                     /locus_tag="IV247_001621"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAP8777693.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
BASE COUNT         8329 a         4398 c         5378 g         7567 t
ORIGIN      
        1 ataaaaaaag cgttagcaac tatgcttaaa aagcatatcg actgaataaa ctaagcattg
       61 gacaggctga aagactacat ttaaaatgaa atgcgaagaa acaatagaaa ataaagttgc
      121 cttagaacca tagttgaatc acctaacagt cgtgaaggga gaataagaga aatggcagaa
      181 aaacaaaccg caggacgaga tcaattagga gattttgcac caaaatttgc agaattgaat
      241 gatgatgttt tatttggaca agtatggtcc agagaagaat tatctgctca tcagcgtagt
      301 atcatcacca tatctgcatt aatttcagga ggaaattttg aacaattgcc agctcatctg
      361 aagattggca agcaaaatgg cattactagt gaagaaatag cagaaatcat tacccattta
      421 gctttttatg taggatggcc aaaagcttgg tcagctttta gtttagcaaa agaaatctat
      481 ttagaagaaa atagcgaaga taagaaagag gcgtaaatga cgacaaaaca cgaagaaata
      541 cacaatggcg tgatttttcc agcaggtgaa aaaaacgaag cttacgctca agtatttacc
      601 gggtaaagct atctgaatat gttagtgaat gatcccgaag tttctgttgg tgtaggtaat
      661 gtaacttttg aaccaggttg tcgcaatcac tggcatgtcc ataaagacgg ctatcaaatc
      721 ttacttgtga ccggaggaga aggctggtat caggaagaag gaaagccagc aagacgatta
      781 atggctggag atgtcgtcgt aacgcacaaa gggattaaac attggcatgg tgcgacaaaa
      841 gacagttggt ttgaacattt ggcgatcact gctggtaatt cagagttttt agaacctgta
      901 acagatgaag tgtatgaagg attggaaaat caataatgtc aagcttgaac agctcctgaa
      961 agtagatttt aatcgaactt tcaggagctg cttttttgat tcagtctttt ataaatagaa
     1021 aatagggtta ataataggaa tgagaagcac gtcgaaagat cttaatattg ataatcggct
     1081 gggcatacat gatttccacg tcatcaatca aactacctaa cagatgataa tcttcccgat
     1141 catgtcgggg attacctaat agttcttgat aatagatttc catactaggg tcccgaggat
     1201 tgttcagcaa atcagcaagt acatctaaat cttctggctg agtttctgcc gtaccctgta
     1261 tctctatgaa aagcccttcc tcagcaaatt ctactccaat acgtatttca gttatttcat
     1321 gggaatataa ataagtaaga agctcatcga tgattctttt tgataattct ttttccaagt
     1381 gtcttttttt cctttctgac agcggtaaag ttatctagta tcggtacaag gatacctgca
     1441 acgaaaccag ttgaaaaacc actgttatat agatttagtc ctccgtgcaa agtgctgacg
     1501 ttatggacaa gagcaatatg tagtacacca gctattacac cgtaaaaact gccgtaaaaa
     1561 ccgcttatgg gcgcgagacc tgtcccaaac acagctgcaa caagcatgcc agtactggtg
     1621 atctcatgaa aagttccaaa caaagaagca ataaatattc cgactagtat cggaaaactg
     1681 ttttttaaat gacaaccaaa agcactgaaa cccaccgcag ataaaatagc accgatgaca
     1741 ggtccattta actgaccgcc aaccagcaaa acataagaag acagcatcag accaactaag
     1801 cctatgttga tcaaagtagc accaatacca gaattagcaa tgaaatcagt tgtcaatttt
     1861 ccagatgaat cgaaaatttc tcgaatcccc aaaagccgaa aggaattata atagaaacca
     1921 attccgactg tcagcgaaaa aaagccaaaa ataatccaaa tgagaggaaa gtgatattca
     1981 ttgaaaacca atgtattttc gacgattgaa tagctgaata acctcaaaac agcagtaaaa
     2041 gtcatcgcaa tcaacccaga ggtgaatcca atattataaa gtgtgaatcc acgatggaaa
     2101 actaaagttt gagcagctaa gggcggaaga agtagaccaa ttgctatgcc agctaaattc
     2161 cccaataaaa tacctatcag taaaggaaaa cgcataccaa aagtgatata actaacgacc
     2221 ggtgataatg cgctaccaaa cagtccgatc atgatgtact gagaaaaagg tttattgaca
     2281 aatacagcgt atagatacac acctaaaatg attgagatag aattataaag gtttttgcca
     2341 aaaaaagaga aaccggtaac tgttagaatc gaagcaaata agggaccgtt taaaatcacc
     2401 ttgtgccgat aagctaaaag cacagaaagt aaggtcaaaa tccctacatt tatgaatgcg
     2461 cttccgaaac cgcctaaagc aaaataatct gtcaaaagat tgcttggcga ggtaagaata
     2521 gttagcaatc cactccaatt atcagtagga gtattagaaa gcaagccaat aaaaagaaaa
     2581 aaaacacctg taaaaccaag ataaaaatac tttcttttct gtgaaacgat tcgtttttga
     2641 ggatgaatat aaataacgtc agtttcgttt tccattacac accttctttt tcattttctt
     2701 taactataac ttgaaatgaa agatttggac aactatcatt taggtcaaaa ctaaaaatgt
     2761 aagggaaccc attgaattta accaaatttt tttaattttg caaaaaaaag ttaaagaaga
     2821 ctgaaaaaaa atttaaaata taacgttttg agtaaaatgt ttgcaaaaaa tgctaaaaat
     2881 agggtacaat aaaacaacat atactgcagt aaagaagtgg gggagtttta atgagaagaa
     2941 aagtctatac caaggatcaa attctaaaag ctgcttatga tgttgttgca aaggaagggt
     3001 ttaaaggatt tactgcgaga aatattgcta aaaagatggg gatttcgaca cagccgattt
     3061 atctggaatt caaaaacatg gaagatttaa aaaatacgtt gctggattct atctttgaac
     3121 aactaaaaga agatgttttc cctattgaac gtactggtga taaaatcatt gatctcggtt
     3181 tgaactatat ctactttgca aaaaagcatc atagtcttta catggcattg tacattgatg
     3241 aacatgggga cggaaagaaa atgcatgatt tttcatacaa ttatttccgt gaaatagtta
     3301 aagaagatga aagatacaat caattatcgg atgaatatgt cgatgctctg catactggta
     3361 catgggtagc agtgactggc ttagcgtcat tgatgtcatc aaacatcatg catccaacag
     3421 aagaacaaat cattgcactg atcaaacgga caattgactc gattttagca ttagataatc
     3481 ctgaaataat cagatagtgt tctatcagta tgaatttgat cggtaagtcc gttggctgca
     3541 aactgtcaat ggactttttt gctaaaaaaa gcgatttctg ttatttatct attagaaata
     3601 ggcttgacat ttcgtgaaaa aagtttaaaa atagtactta cggaaggttg gcagagtggt
     3661 aatgcaccgg actcgaaatc cggcgaaccg gctaataccg gcgcgcaggt tcaaatcctg
     3721 taccttcctt atatcaacga ttagaggttg tgaagaagct gtcctgcatc aagtaataag
     3781 aacggccaaa caaaaatagc ttttcctatt ttttcgtttg attgggctta ttaccgcaga
     3841 tgcaagcttt tgaacaccgt ttgttcggaa taaggggctg tgacaagact tttgtcacag
     3901 ccccttattg ttgtactttt aacctacaat attttttgat tccccctgag tactattttt
     3961 tataaatgct agtaaatcaa tcttctttgg cctttttttg ataaaaaaaa tactagatgt
     4021 aagataaaag tgtaaaaacg atttgtttta ggacgggaaa cgatgtgtac cgcaatcact
     4081 tatgcaacga aagaccatta ttttggaagg aattttgatt atgagatgtc ttataatgaa
     4141 gtggtcacta ttactccaag aaattatcgt ttcgatttta gaaaggtaaa aaatttagat
     4201 aaacattatg caatgatagg aatagcagct ggggtatcca actatccact ctattatgag
     4261 gctacaaatg aaaaagggtt gagtatggca ggactgaatt ttcctggcaa tgctgattat
     4321 aaagaattac aagaaggaaa agataacgtt gcaccatttg agtttattcc atggatacta
     4381 gggcaatgct caaccataga cgaagctaaa gaattattgg ctactatcaa tctagtcaat
     4441 atcgatttta gtgaaaaact acccttgtca cctttacact ggttattagc tgataaagaa
     4501 aaatcgatag taattgaaag tatgaaagat ggacttcatt tatatgataa tcctgtcggt
     4561 gtgttgacaa ataatccgcc atttgattat caattattta atctgaacaa ttatcgctcg
     4621 ctatcaaatg gaacaccaga aaatcatttt tcaaaccaga tcagcttgaa tgtttatagt
     4681 cgtgggatgg gaggattggg cttacctgga gatctctctt ctgtttctcg atttgtaaaa
     4741 gccacattta ctaaaatgaa tgctgcatca ggtgattcag aatctgaaag tatcagtcaa
     4801 ttttttcata tactaggctc agtcgagcaa caaaaaggag tatgcgatac tggagaaaga
     4861 aaatacgaat atacgattta ttcttcttgc tgtaacgttg ataaagggat ttattattat
     4921 cgtacgtatg aagacagtca aattactgct gtaaatatga ataaagaaga cttggatcgt
     4981 catgaattgg tcagttatcc agtcataaaa gagcaacaga taaactatgt caattaatta
     5041 atgaatagat ccgtttggta tcatattttc tgatacagtc ataattagac acgatcttta
     5101 ggtcgtgtct ttttttgctg gtttttttgg aaatagcatc cactaatatt tttttaacac
     5161 gaagagaata aggtagcctc aaaaaagaaa acatggtaaa ttagatattg ggactttatt
     5221 ttctgtaaaa acagaaactt tcatcttttg ttttcacgtg acgaaattgt aagagagaaa
     5281 atattcgtcc gctatttatt ctatgataag atgcatatga atgaaataaa cagtaatttt
     5341 atattaggat gtgaaagtta aatgtgcggt attgtaggtt ttgttaacga caaggacaac
     5401 aaaaaaacaa ttattaatac aatgatggat cgaatcgttc accgtggacc taatagttca
     5461 ggagaatata tcgataaaca tgttgcttta ggattcagaa gattaagtat tattgactta
     5521 gagggcggta ctcagcctat ttacaatgaa gatcggacaa agatcattat ttttaatggc
     5581 gaaatctata attaccagcc tttgagagaa gaattgattg cagctggcca cgtttttcaa
     5641 acacatgcag atacagaagt ccttttgcat ggttacgaag aatggggaac agaattattg
     5701 caaaagatcc gcggaatgtt tgcttttgct atttgggata atgaaaagaa tgagttattc
     5761 ggtgcaagag accactttgg gatcaaacca tattattatg cagaaatgaa cggaacattt
     5821 atgtttggtt cagaaatcaa aagttttctg cctcatcctg attttaacaa agaattgaac
     5881 aaagaagcgt tgaaacctta tatgactttc caatattctc cattaaacgg cgaaacattc
     5941 ttcaaagatg tctatcgttt gcctgaaggc cattattata cgtataaaga tggaaaaata
     6001 gacattcagc aatattggga tgcagatttt gaaacaaaag aaacgcattc ccgacaagaa
     6061 tggatcgaaa aaatcgacga aaccgttcaa gcttctattg aagcacatac ggtcagtgat
     6121 gtagaagttg gttctttctt atccagcggg gttgattcca gttatgtaac ctctgtattg
     6181 aaaccagatc attctttctc aatcgggttt gatgataaaa cctataatga agcgattgaa
     6241 gccagaaaac taaccgaact attggactta gataatacag cagcagtgat cgacggagac
     6301 atgtcgttca aagcattccc tttgatccag tatcacttag atgaacctga ttcaaatcct
     6361 tcttgcgtac ctttgtactt tttagcaaat cttgcttcac aaagtgtgcg ggtagtccaa
     6421 tctggagaag gggcggatga actgtttgcg ggataccaaa cgtatggatt ccatacgaat
     6481 tcaaaattca tccgtgtgat tgctcaagga ttgaaaaaat taccaaaagg aacacgctat
     6541 aatttaggac gtaaaatcgg aaaaatgaaa aatttccatg gacggatcca tttatatgaa
     6601 tcacttgctc ctgcaaaaga atattttatc ggtcatgctc gagtgttcga agaatcagaa
     6661 gcatcagaag tgttaacacc aaaatatcaa acagcaccat ctgtggatga aatcatgact
     6721 gttcattacg agaaaacaga aggaatcaaa gatgaagtca ataagatgca atatgtcgat
     6781 cttcatcaat ggatgccaaa agatatcttg ttaaaggcag ataaactttc tatggcaagt
     6841 tctttggaag tccgagtacc tttattggat atcgaagtca tgaaattggc tcaacaaata
     6901 ccaagtaagt ttttattgaa tcaaaacaac acaaaagata ttttcagaca agctgcgaac
     6961 aaacatcttc cggaaaaatg gtcgaatcgt gtgaaattag gattccctgt gccaatcaaa
     7021 gcttggttga aagaagaaca cggctacgaa caagtcaaag cattgttcga agcagatttt
     7081 gcaaaagaat tttttgatca agaaaaaatc atgaagttgc tagatgacca tcacgaagga
     7141 cgtaaagaag agcaaagaaa aatctggaca atcttcagtt tcttgacatg gtataaagtc
     7201 tactttgtag atgaaacgat tccgcaagca gaagcaatcg actatgtgac tgtttgatga
     7261 aaatcaaata tcaataaaaa agaggttttc tcctaccgtt taacactgag tgataagaaa
     7321 caatttcaaa aaacagctct tcccgtttta tagatattat gacttatggt tcggagttgg
     7381 cagaggaaca ccattgtttc gaacataagg gggttgtgac acgattttcg tcacaacccc
     7441 tttacatatg ttcaataatc gatttctttt tcttttgtcc catctttttt ataaatgaac
     7501 aaagttgatt ctttgttttg tgctacttgc tttccttttt cgattgcttc tttttttgta
     7561 tcaaaatggt tgcttgcctt ttttgcccct ttagatttca cgatccattg ttcttcctca
     7621 tattctacga tcacatctga atcaagcagt tttccagcac gaggattcgt atcgtgttca
     7681 tccgtcttgc ttggattttt ttctttttcg aaagttcgtt tttctgattc actagcatct
     7741 tcataccatt tttttgcttg atcaatggca attgggatcg cacggttatc agggtaccct
     7801 tcatctaata atgcatttgc gatgtcgatt gctttttttc ttgtcaattt atctaagttt
     7861 ttcattgatg caggaaagtc cttcatgttc catggcatat tagtatcccc ctttattaaa
     7921 aaacataatc tatataaaca agtgtaaggg tatttataca aaaagaaaaa gattttgttt
     7981 gagaagtaaa accgttgtat ttatcgtcaa gtagttttac ttaacaaaat cgtgaaaaac
     8041 actagcaatc tagaaaaaat catggtaaga taatcaagtc tgagagtcat cttagaggat
     8101 tcttgtaaaa atgggaggga aaaacatgcg cgtaaacatc actttagagt gtacttcttg
     8161 taaagaacgt aactatctaa caagcaaaaa caaacgtaac aatcctgatc gtttggaaaa
     8221 acaaaaatat tgcccacgcg aacgcaaagt tactttgcac cgtgaaacaa aataatgttt
     8281 ttaaagttct caattccttg ctggtcaaag aattgagact ttttttatct caaaacatct
     8341 aaaatcataa ggagcggata atatgtttat ttccatgtgg gcacaagata aaaatggtct
     8401 gatcggcaaa aatggtttgt taccatggcg attgccaaat gatatgcgtt tctttcgcga
     8461 acatacaatg gatcgaattt tagtgatggg aagaaagacc tatgaaggaa tgggggatct
     8521 ttcacttcct tatcgccata tcatcgtgtt gacaacgcag ccaaattttc gcacgaaaga
     8581 aaatgcggaa gtaatgcatt cgatcgatga acttttgtcg tatagtcaaa cgatctcaga
     8641 agatatctat gtttcgggtg gtagtcggat ttttcaagaa cttcttcctt acactggtcg
     8701 gatctggcga acattgatcg atagcacgtt tgaaggcgat acgtatattg ggaatattga
     8761 tttttcagac ttcgccctta tagaggagta tgaggggata acggatcaag aaaatctata
     8821 tgcccatcgt tttcaaaaat gggaaagagt aaaaaatagg gttgcttcaa aagaagaata
     8881 aaatctatgc gtctagtttg ttaataagaa aaaaagcata tcacgactaa tcgtgaagat
     8941 gcttttttta gttaaaatca atttagtttt tctttgatat cattgaccat ttcctgactg
     9001 gcaataggtc cgttatatag ccaactagat tcaggtccga attgcagtac atgattgtct
     9061 gtcactgctt taagattctt ccagcttggt tcattgaaca tggcagcatc tttgtcactg
     9121 ttcacaagga tgatgtagtc agcatctaat tcagctagct tttcagagga aacagcagac
     9181 cagtcagaag tggctttttt ggagacctct tccactagat caggaacacc aaattccaga
     9241 tcgtcgtata cgattcttcc acttgaccga tttttgctga ccataaaggc tgagttgttg
     9301 gtcacccaca acactgcagc tgtcttgttc tcgattttat ctgctagttc ttctcgagtt
     9361 ttcttgatgg atttttgata atcggaaatg atcttttctg cctctttttc ttttgccaaa
     9421 gctttgccga catcttttag ttgttcttcc catgtgacat ctgttccgtt ttttacaaca
     9481 taagttggcg cgattttgct atattgttcg tatttcccgc cttcgacagt agcggaagaa
     9541 gaaatcaaca gcaaatcagg ctcaaagctt aacactttct cataaggaag gtcataagaa
     9601 atcgttggga catcctttaa atcatcttgc agataatctt gaatggaacc actgccaaca
     9661 gtccactggg cgataggttt ttcccctagt gcaatcagat agtcttctaa gtaactgccg
     9721 atgatccgtt tgggcgcgtt tggaatctct actttatgtc caagtgtatc tgtgactgta
     9781 tgagtactat tggatgaagt cgtcgaattg gcagttgtag ctgtttcttt atctgaagag
     9841 ggggagcaag cgctcagtac tccagccagc agcaatatgc tagataatgt gaatactttt
     9901 gtttttaatt tcatttcttt cctcctaaca taattgagaa tctttctcat tggttattat
     9961 tcctgatatt tcgaacgctg tcaaccgctt catttggcta tagatatgaa agtagcgctt
    10021 gacgaaatgt tgaaacagcg tcataattga gaatgatttt cagcgaaagg atgggtgaaa
    10081 tgacgagatt aagagcagct gacattgcag ccggctacga aaacaaaaaa atcattgaaa
    10141 acatgtctgt tacgattcct gaagggaaaa tcactagttt gattgggcca aatggaagtg
    10201 gaaaatctac tttattgaaa agttttaccc gtatactacc tgtgaaatca ggaaagatca
    10261 ttgtcaataa ctctgatatc tctacataca gttcaaaaga acttgcaaaa aaaattgcat
    10321 tattagcaca aactagtgaa caccctacag gtatgacagt agaagaagta gtatcttacg
    10381 gacgataccc ttatcaaaaa ctgttcagtg gcatggacac gcaagatcat gaagcaattg
    10441 agtgggcgtt ggatgctaca ggtctagctg gatttcgtat gcgggatatc gcaacgctgt
    10501 ctggtggaca aacccagcgt gtctggatcg ctatggcgct tgctcaggaa acagatatct
    10561 tgatcttgga tgaaccgaca acatttttag atccggcaca ccaattagag atcctacagt
    10621 tattgactga aatcaatcaa atgaaacaaa caacgatctt gatgtcgatt catgacatta
    10681 atcatgcttc acgtttttca gattatttat tagcaatgaa aaacggtaag ttgatcacag
    10741 aaggtacacc tgaagaagtg atcacaaaag agtgtatgaa aaaaatattt gagattgatg
    10801 caacgattgt cacgcttcca agaacaaata aaccagtttt tctaacctat gatttgttgc
    10861 caaaagagca ggaggatcaa ttatgaggac tatgaagaaa aatagactcc tgcttttggt
    10921 ctgttttgtt cttttgatta ttttttttgt tgctctttca aatggcgcag cgactgtggg
    10981 aaaagcagat ttttggcagg cgttgtttga ttttgataga tcaaaacaaa gtcaccaaat
    11041 catacgaaat attcgtttgc ctcgtgtggc gggagcattt cttgtaggga gttgttttgc
    11101 attgagcggt gcattgatgc aaggagtgac aagaaatcct ttagcagatt caggcttact
    11161 aggaatcaac gcgggtgctt cgttggcaat ggcccttagt tttgcttttt ggcctaaagc
    11221 ttctgatttt tctgtgtttc taatgtcatt ggcaggttct tttgctgtaa ctttgctcgt
    11281 atttggtttt ttgcagtttt cgaaaatggg catgaatcct ttacagctga ttttagctgg
    11341 cgtagctatt agttcttttt ttactgctat cagccaagca cttacccaat tctttaatct
    11401 gcagcaggat cttgcttttt ggtttgtagg cggagctgcc aatatcacat ggaaacaatt
    11461 agcagtattg tttcctttat atatacttgc tgttttggtt tctttttcat taggaaaagc
    11521 aatatctttg attcatttga gtgaaacaca tgcattagct cttggaagtc atcctaaacg
    11581 aacgcggttg attgttttct gtctagttgc agtgttagca gccattgccg tttcgttagt
    11641 cggacctgtt tcatttattg ggctgttggt tccgccagtt gttcgcggaa tggttggaac
    11701 ggactatcgt tatgttctac ctgtctcttt tttagttggc ggtggattgg ttatgttggc
    11761 ggattttaca gcgaggatga tcaatccccc ttttgaaaca ccttttggat tagtcattgc
    11821 attgattggc gttccgtttt tactgataca agtaaggagg gaacaacggt gaaaaaaata
    11881 tattggacgt tctatttctt gctttttgta cttgtcatcc tgtctatagg tagtttaatg
    11941 attggtacgc cttttattta cctggatcag ctttttgcca cgttcatagg gaaagaatca
    12001 tcttctttgg cgctgattgt tagagaattt cgagttcctc gattactgat cagtttatta
    12061 gcaggagctt gtcttggcgt ttctggttat atcttgcaag gagtaacaag aaatgaattg
    12121 gcagattcta gtatattggg cattaatgcc ggagcaggat ttttagtgat gctttattta
    12181 ggtttctttt cgcactattc gctgccgttt ctattgctag ttgtagcctt tctaggtggt
    12241 gttttagctg cttgctgtgt ctatcttgcg gcctatgatc gaaatggctt tttgggtatg
    12301 aatcgaatct tattgtccgg tattgccgtt aatgccggcc tttctgcatt aacattgcta
    12361 tgcacgatcc aattatctaa ggaaaattat ggatttgtga atgcatggtt agcgggatca
    12421 atctggggtg cgagctggcc ttatgtttgg gcattgcttc catgggcggt tatcttaatt
    12481 ccgctaggag gactttatgc acagaggatt gtattgctat catttggtca agaacgcgcg
    12541 caaggattag gccttaacgt ggctaaaagt caaaaaatcc ttttgttgtt agctgttgct
    12601 ttggcaagcg gtagtgtagc ggtgacaggt agtttatctt ttgtaggact actagctcct
    12661 catgctgcga agctagtcgt acgtaaagaa aataactgga cctttgttct gagcggtttg
    12721 ttcggttcca tttttctgct tagtgcagat atggttgcta gagtgattct actaagtggg
    12781 gaagtaccta caggaatttt gattgccctt tttggtgcgc catattttct gtatgcactt
    12841 tttatcagac aaaaacacgc gctgaacagt tgaaattcat taaaaaagga aggccaatca
    12901 tttctaggtc ttctttttat tctatttatg catttatttg aaagttcgat tgctattttt
    12961 ctttttttcc tataccattt tatgagagaa ggaagggtga gtttttggac caattgaaag
    13021 taagattacg ccggaaagga atcgaaaagc tgaaaaatct ggccaaaagt ggcaagaaaa
    13081 aggaaaaaga acagcgaatc tataaacagc tattcgccag cgagtactgg aagtcagcaa
    13141 aagtgatagg gacgacttta tcgaatgaat ttgaactgga tacacagccg gttatccaac
    13201 gagcagaaaa agaacaaaag aaaatagttg tacctaaaac tttgccgaaa cgtcagatgg
    13261 cgttttatga aatggatgaa cagtccgttc ttgtacgttc gagttttggt gttcttgagc
    13321 cacaatcaga gaagctctat ttgccggaga tgatcgatct cctcattgta ccaggtatta
    13381 tctacaatag agccggttat cgaatcggtt ttggcggtgg ttattacgac cggtatttaa
    13441 ataattttca taaaaaaaca tgtagccttg tttttcgtga acaattgaat gaacaatggc
    13501 aaccggagag ctttgatcag aagatccaac aattgtttat tgactaaaat gaaatgggag
    13561 gttttcatga attatcaaca gcaaataaaa ttgcgtgcat ggttaagacg accgttatgg
    13621 acctatgctt ttttagggat acagacagcg gtcttcctca tgatggaatt atttccgcga
    13681 cttgagatcc cttattatgc cggaatgtat ggtccttatc ttgttcactt caatgaatgg
    13741 tggcgtttag tgacaccaat cttcatccat tttggactga tgcatttcgt aatgaactca
    13801 ctgatactgt attttatggg gcagcaaatc gaggcaatct atggccattg gcgttttttc
    13861 ttgatttata tgttcagcgg gataatggga aatacagcaa gctttgcttt taacgaagcg
    13921 aatgttctgt ctggtggtgc aagtacttcg atattcggtt tgttcggtgc gttgtttatc
    13981 ttagggtttc attttaaaca taacccagcg atccagcagc tagtcagaca ttttctttta
    14041 tttattgtgt tgacatttgt ttttggtctt ctggatacct ctgtagatgt ttggggacat
    14101 gtcggtgggc taattggcgg attagtgtta gggaatgttt tgggtctacc gaaacaacag
    14161 acaagctatt cgatacacca aaggattctt tctacactag tttttgtatt tctttttgta
    14221 atatgtattt tactgggttt aaaaaaatat ggtttacttg tataatatga aggaagtgct
    14281 taaagatgga gagcttatat gatgtacagc agctgttcaa aaaatttggc atctttatct
    14341 atgtgggtgc acgcatctat gacattgaat taatgactat cgaattgcgt aatctgtatg
    14401 atagccgttt gattgatcat gatacataca tgacagcgtg gcgcatatta aaaagagagc
    14461 accgaattga agaaagccga aagaaaggaa atttgtaatg gacaagaaga ttattgggat
    14521 tgatttaggt ggaacaacag caaaatttgc aattttaaca ccagaaggag agattcagca
    14581 aaaatggagc atcgatacca atattttgga tgatggtaag catatcgttc ctgaaatcat
    14641 tgaatcaatc aatcatcgtt tgaatttata taatatgaaa gccgaagact ttatcggaat
    14701 cggcatgggt accccaggaa gcgtggacag cgaagctgga acggttattg gtgcgtataa
    14761 cttaaactgg acagaagttc aatttgtgaa aaaactgatc gaggcaggaa caggaatcaa
    14821 attcgctatc gataatgacg cgaatgttgc tgctttaggt gaacgatgga aaggtgctgg
    14881 agaaaacgat ccagacgttg tattcgttac tctaggaaca ggtgttggcg gaggtatcgt
    14941 tgctggcgga aatttgattc acggcgtgag cggagctgga ggagaaatcg gacatatcac
    15001 tgtggatcct gaagggttcg aatgtacttg cggtaaacgc ggatgtttag aaactgtatc
    15061 tagcgctaca ggtgtggtac gcctaggtcg tttcttggca gaagaatacg caggggattc
    15121 aaaactgaaa gcaatgctag acaacggaga agaagtaaca agtaaagata tctttgaaat
    15181 ggctcaagaa gacgatccgt ttgcattgat ggttgttgat cgcgtatgct tctacttagg
    15241 tttagcttgc ggaaacttgg ggaatacatt aaatccgtca agtatcgtgt taggtggagg
    15301 agtatcggca gcaggagaat ttttacgtag ccgtgtagag aaatatttta aagaatttac
    15361 tttcccgcaa gtaagagaat caacaaaaat caaattagca gagcttggta atgaagctgg
    15421 tgtcattggt gctgcctcat tagccttgaa atttgcttaa aaaaacggag gcattacatg
    15481 gttttatggg taattaatat cattttgcta ttgattgtgc ttgcaatcat cctttgggaa
    15541 gtctatctac gagtaatggc caaacgttca gcaacgactt tgacagaaga agaatttcga
    15601 gaaggaatga gaaaagcaca agtaatcgat gttcgcgaaa aagatgtgtt tgatgctggg
    15661 catattttag gtgcaagaaa catcccttac actgttttga aagattcatt aggatctatt
    15721 cgaaaagacc agcctgtcta tatttacgat caaaagaaaa gtttgagcat ccgtacagca
    15781 aataaattac gtaaagcagg ttatcaagag atttatttac ttaagggtgg atatgacgga
    15841 tggtctggaa aaatcaaaag taaaaaagta taaaacagat gattgtatga cagctttaaa
    15901 aaccgaatca atcattagat tttgaccaag atcacatgaa cggcgcaatg gatagttgtg
    15961 ccgttcattt tttttatttt gtgctattat ctacatatat aagctaagcg aaaaggtaga
    16021 aagaaggaaa caactatgat caaattaaaa cgtgtttacc ttgatgctga aaaagaggat
    16081 ggctatagaa tactcgtgga tagattctgg ccacgaggaa tttcaaaaga aaaagcgcag
    16141 ttagatctgt ggttgaaaga aattgcacct agtaatgaag cacgcaaaac ttttgaccat
    16201 gtaccagaac gatttgcttc atttgcaaag aaataccagc aagaattgat tttcgaagaa
    16261 gaaaagaagc aagctatttt taaactcagt caacttatga aagaacatcc agtcattact
    16321 tttgtttatg gtgcaaaaaa tgaacatcag aaccatgctg ttttattgaa aaaatttatc
    16381 gaaatggagg catgcaaatg aaaataggga tcattggtgc cggacctaga ggattaagtg
    16441 tagcagaaag gattctccat aataatcagg acagacattc tcttgaactt tttcttttcg
    16501 atcccaatgg tcctggaggt cgagtttggc ggttgaatca gccaccggag ttattaatga
    16561 attctgtttc tcaacaagtg acgcttttta cagatgaaac attgtccagt ggaggattgg
    16621 taacacaggg acccaacttg tttcaatgga gtaaaggacc tgctacagat tatatcgaac
    16681 agacggtaac caaaaataag caactaatta tagaagaaat caatcgtctg gaaaaagatc
    16741 agcaatctac tcgatgttta tacggactct atcaacgctg gtttttctca aagttgcaag
    16801 aagaatttcc agcttctatt cagtttgttt attctttagt aaaaaaagta agcaaaagaa
    16861 aagatggtta tcttttacag acagatcagc aaagctattc tatcgatcag cttgttttag
    16921 caagcggtca ctgggaaaat gatgagacag aagaagaaag agtgtgggta gactacgcaa
    16981 agaaaaatgt tttactgtat cagcttcctt ctaatcctgc agatgtagat ctttcgaaga
    17041 taccgcctaa agaaaatgtc tttttaagag gtctaggctt aagttttttt gattatgtcg
    17101 gattgtttac agtatcaaga ggagggcgct tcgagaacga aaagggaaaa ctggtctatc
    17161 atccatcagg caaggaaccg attgtataca gtggatcaag aagggggctc ccttattacc
    17221 ctcgaggaag aaatcaaaag caaggcggag caatggcatg gccacggtta ctaacaaaag
    17281 aaaacctgga gcaatggcat cagtcgggat ttttgactgg cgaaacattt tttgactatc
    17341 tgaaaaaaga cgttgagttg ttttattaca agaaaatcat cgaagaatat cacttgccga
    17401 ttccaaagaa aaattttgag catgattttc tctccctctc ttcagaggag tgtctcggaa
    17461 agcatccagc actaatccct tatagatggg attggtcgta tttagaaact cctcttgcct
    17521 atcagaatga gcccttttca gaagctagcc gaatatttat tgaagggcag ataaaagaag
    17581 cggaaaaagg caattgcaca ggagcatttg cttcaacatt tgatgcatta aaagactggc
    17641 gtgatccaat ccggcaggct attgaatggc acgttttttc tgctaaggaa tacaaagaaa
    17701 tactatgggg atggtttact catctaaata gctttttaac gatcggtccg ccaatcattc
    17761 ggacgaaaga attagcagca ctgatagatg cgggtatttt tcatttgatc gaaccgccat
    17821 ttgatattca gcaaaagcac aaccgctatg ttatttctct acaagataaa gaaatcagca
    17881 gtcgctattt gattgaagct cgattgccac aaacaagctt ttctcgttca agtaatcctg
    17941 tcataaaaaa tttgaaggaa aacaaacttg ttcgcccttt ttcattttca gataatacag
    18001 gaagttatca ttcagaagct atagatgttg agcgaggtac gaatcgcgtg attgatgctt
    18061 caggggaaat ccagtctgac ttttattgct atggaatacc tgttgaaggt gtggattggc
    18121 taactgcagc agtagctaga ccttatacag atccatggaa tctgagacag gcagataaaa
    18181 tagcacaact tatattgact gacaaataaa aatacgcatg atgacttatc tgttttttag
    18241 aaagtcttca tgcgtatttt taaataatta ttaacgagaa acgcgtttac gcattgcttt
    18301 tttacggttt tcttcgatca ttgcttcttt ttcgtcaatt ggatcgatca ctgttttttt
    18361 cacagaagca accaaaccag caacggcagc ggctgttgcc agacttccta ctaatattcc
    18421 agatacaaat tttttcattg aatccacctc atttattatt tgttatatct attatggaac
    18481 aaaagagaaa aaaaggctag ttttacgttt gtttgtaaac ctttgcaaaa aatccttgaa
    18541 aatgtttact aaatgtttta ttataaattt accatatgaa tttacgaaat tttactttgt
    18601 ttgttttcac cataaaagta aacttcgttt tttaaattat atagaatcaa caaacacaat
    18661 tttactaatc aaaggagtgt tttttatgac aattttagta ctaggcggag ccggttatat
    18721 cggttcgcac gctgtagacc agctagttca aaaggggtat caagtagctg tggtagataa
    18781 tttgctcaca ggtcacaaac aagcagtaca tccagatgct catttttatg aaggagacat
    18841 ccgggataaa gaatttctac gttccgtatt tgaaaaggaa ccgattgaag gagtaatcca
    18901 ttttgctgca agctctttag taggagaatc tgtcgaaaaa ccattgatgt atttcaacaa
    18961 caacgtatac ggtatgcaaa tcttacttga agtcatgcac gagttcaacg tgaataaaat
    19021 cgtattctct tctactgcag caacttatgg agagccgaaa gagtctccaa tcacagaaga
    19081 tacaccagct aatcctaaaa atccgtatgg tgaaagtaaa ttgatgatgg aaaaaatgat
    19141 gaagtggtgt gaccaagcat atggcatgcg ctatgtcgct ttgcgctact tcaatgtagc
    19201 cggagcaaaa gcagatgcat ctatcggaga agatcataca ccagaaacgc atcttgtccc
    19261 aatcatttta caagtagctc ttggtcaaag aaaagccttg gctgtttatg gagacgatta
    19321 tgatacacca gatggaacat gtatcagaga ttatgttcaa gtagaagatt tgattgctgc
    19381 acatatttta gctttggaat acttgaaaga aggaaatgaa agcaacttct tcaatttagg
    19441 cagcagcaaa ggatactcag tcaaggaaat gctagaagca gctagagaag tgacaggaaa
    19501 agaaattcca gcagaaatag caccaagaag agctggagat cctagccgat tagtcgcttc
    19561 aagtgaaaaa gcgagagaga ttctgggctg gaaacctgaa tacacagata tcaaagcgat
    19621 tatcaaaaca gcatgggatt ggcatgtcag ccatccaaat ggttatgaag aataggggaa
    19681 tgagatatgt caatcagtca aacgatttgt gattttgcca cacttgcgat cgaagctggc
    19741 ggatggatgg aaatggaccg cttatattta cagaatcgtc ttttagggat ggtaggagaa
    19801 gatgctctag aaagcattga agtaagacca gttaaggaag aatctgtaga cttattggat
    19861 cagcttgtcg ctaaggcaag agaaaatggt gttctttcag aattgccagc agaacaagag
    19921 atttttgaag cgcaattaat ggattttctc acaccgccac cttcagtcgt caatgcattc
    19981 tttgcacagc actattcgaa aagcccagag gaagcaactg agtatttctt taatttaagt
    20041 aagaaaaatg actacatcaa aacaagagcc atcgcaaaaa acatcaaatt tccggcagag
    20101 tcggagtatg gtgaactgga aatcacaatc aatttatcta agccagaaaa agatccaaaa
    20161 caaatcgcag cagaacgcga ggcggtaaaa gtagactatc ctacctgcat gctttgcatg
    20221 gaaaacgaag gatatagagg tcgcttgaac tatccagccc gcaccaatca ccggattatt
    20281 cgaatgaatc tagacggtga aagctgggga ttccaatatt ccccttatgc ttattacaat
    20341 gaacattcga tcattttatc agaacaacac cgcccgatga aaatcacaaa ggaaacattt
    20401 gctagactgc ttcggattac tgaagtcttg cctcattact ttgttggttc gaatgctgac
    20461 ttaccaattg ttggaggatc gattctttct catgatcatt atcaggcagg acgtcatgaa
    20521 tttcctatgg caaaagcacc gatagaaaaa cttatcaaac tagaagatta tccgcaagtc
    20581 atcgcaggag tcgtgaaatg gccgatgtct gtgatccgtt tgcaatcaac agagaaaaac
    20641 ttgcttattg aagcagctaa ctcagtattg gaaaaatgga aagcatattc ggatgaaaca
    20701 gtatctatca gagctttttc agcagatggt acaccacacc atacgattac gccaattgca
    20761 cgaagaaggg acaattactt tgagttggac cttgtccttc gagacaacaa tgtttcagaa
    20821 gaacatcctg atgggatttt tcatccacat aaagatgtcc aacatatcaa aaaggagaac
    20881 attgggttga tcgaagtaat ggggcttgct gttttaccac ctcgtttgaa accagaacta
    20941 gtagaagtag agcatttctt attaggagaa gaaaaccaga tggctgaata tcatcgtccg
    21001 tgggcagaac aattaaaaaa agaacatcct gatttgacga aagatacggt caccgatgtc
    21061 gtacaaaaat ctgtaggtca ggtttttgca cgtgtactgg aagacgctgg agtattcaag
    21121 cgagatgaaa aaggacaggc agcgtttgaa cgatttactg cgattttata aacgtaaaaa
    21181 aggaggagag acaaatggca acaattaaag acattgcgca gttggcagga gtttctcctg
    21241 ctactgtttc aagagttctc aactatgatc ctgatctttc cgttggctta gccacaaagc
    21301 aaaaaatttt tgaagcagca gaacgcttaa attatacgaa acatcgtaaa aatgcagcaa
    21361 atactaagca aacgcttcgc ctgatccagt ggtacgacga tacggaagag cttgaagatc
    21421 tttattattt atctattcgc ctaggaatcg aaaaaaaggc agaagaagca aacgttactt
    21481 tattgaaaga aacgtgggat tccattagtg atcttccggc agatggaacg atcgcactag
    21541 gaaaattcga cgaagctcag ttattagaaa tgaaaggatc ccaaaatgcc ttgcttttgg
    21601 tagattctga cggcacttct caaggaatcg actctctagt tgtcgacttt aagtcaagtg
    21661 ttcaaaaagt cattgaacac tttgtctccc ataaagcgca acatatcgct atgcttgctg
    21721 gaacagaata tacgaagaaa aaccatcagc ggctaaaaga tcccagaatt atggcatttc
    21781 aacaagtttt ggcagaaaaa ggctacgacg aaggtccaat tatcgaagca gatttttctg
    21841 tcgagtcagg atatcaagca gtgaagcagt atttaaaaac gaatccgcat gttcctgatg
    21901 cgctttttgc agctaatgat gcattagcga ttggcgcact gaaagcgatt caagaaaaag
    21961 gtttagtggt accagaagat atttctgtga tgggcttcaa tgatgtcagt gtagcgaaat
    22021 atgtaacgcc ctcgctttct actgtaaaag tttataccga atggatggga gagctagcag
    22081 tggagacgat tcttcaatta agtaattcca aagcaccggt ttcaagaaag attattgtgg
    22141 aaacagagtt gattattcga gaatcaacga aataaaagat ggaaaagctg tgggaaaatg
    22201 gagtgatacc gcagcttttt tgtgtttcag gaaggggcaa aatttgcccg ccagcagata
    22261 atcatgtatt attgtactag ttttgccttt ggtattttca tcctcagaat aaaaaagtat
    22321 gctacaatgg aaggcgagaa tgtcatagca gaaaggatca aatatatgat tacattagga
    22381 atagatactg caaaccaaac tttggcgatc ggtgtggtag aagacgaaca aattcttgga
    22441 caaatacaaa caaatatcaa acgcaaccac agtgtaacac tgatgcctgc aatcgaccaa
    22501 ttgtttgcag atcttcaaat aagtccaaaa gacattgacc gaatcgcggt ttctgatggt
    22561 cccggttctt atacagggct tcgtataggt gtaacgacag caaaaacgat tgcttataca
    22621 ttagataaag agctagtagg agtatctagt ctgaaaacca tcgcggctaa ttgcatcggt
    22681 gtggataata ttattgtgcc catgtttgat gctcgaagaa aaaatgtata cgctggggca
    22741 taccgctgga aagaaaatag attagaaact gtacttgaag atgtacatat ttctgcttct
    22801 gatttgttca cacaactaca aaagctgaat gaagctattt atttcgtcgg gagtgattgt
    22861 cacaaattct tctctgaaat aaaggaaata ttgcctagtg cgatgattaa tacagttcca
    22921 gcatgggata ttccaagtgg tgtaactgtg gctcaattag gtgctttagt tgaaccagtt
    22981 gcagagaaac aagcttttct tcctcgctat ttaaaacgag tagaagcaga agaaaaatgg
    23041 ttggagacgc acacacctgg agacgaaagt tatgttgaaa aaatttagag cgtatgccag
    23101 aagctttttt caagataaaa tgatttatac agaaaaaaca gtcgaacaaa tcgatcgtcg
    23161 ttacgtacta agagaattga ctacagaaga tatcaaagag ctgctggatg tcgaacgaga
    23221 agtctataat ggcgaattgc catggacaaa aagtgccttt atatcagaat tgaagtcacc
    23281 gcttcctcat ctatatctag gtatctttga taatggaaga atgattggtt ttatcggttc
    23341 aagagcgttc ggtttagatt gccatatcac gaatattgcc gttcgaccgg catatcagcg
    23401 taaacgaatc ggttcgctgc ttattgacga aatcgagaac tttgctataa tgaatcgctg
    23461 tgagacgatg tcattagaag tgcgtatgag taatcaagat gcacaaagac tttatcggaa
    23521 attggggttt caagcaacaa cgatcaaaaa aagatattac actgaaacaa aagaagatgc
    23581 gttggatatg ataaaacttt tagaaaatga gtgagattag ttgatttgca gaaaaaaaga
    23641 ttttgatgaa gcgtacttgg cagaatgcgt atggatgatt agcgaaaatg cttatccaca
    23701 tggttcacct tggacgaaaa tgcagttttt ggcagacatc caacagcctc actctgatta
    23761 tctgctttta gctgaagata accagatctg tggctttatt ggatactcga aagtaataga
    23821 tgaagtcgag atcacaaata ttgctgttgc agtttcagaa caaggaaaag ggcatgcacg
    23881 gcatttgtta caattactga ttacggaaca aataaaagat tcgctgcatg tttttttaga
    23941 agtacgtcta tctaatgaag cagcaagaaa actttatgaa tcagaaaagt ttcgtatatt
    24001 aggtaaacgg aaatcatatt atcgcaatcc gacggaagat gcgatcatta tgagcagaaa
    24061 gttaaaaacg gagacgatga aatgaatgaa gaattgattc tggcgattga atcaagttgt
    24121 gatgaaacga gtgtagcggt cgttagaaat ggaacagaga tcttatctaa catcgttgct
    24181 tctcaaataa acagtcataa acgttttggt ggcgtagtac ctgaagtagc tagccgccat
    24241 cacgtagaac aaatcacctt atgcttggaa gatgcactgg tagaagctgg tgtgtcggca
    24301 gaagatttat cggcagtcgc tgtcacttat ggaccaggac ttgtcggctc gttgctgatt
    24361 ggtatttctg cagctaaggc ttttgcttgg gcacaccaat tgccgcttat tcctgtaaac
    24421 catatggcag ggcatatcta tgcagcccgt ctggttaaac cattccagtt tccattgatg
    24481 gcacttcttg ttagcggagg acatacagaa ttagtctata tgcaagaaga tggatcatat
    24541 gaaatcattg gggagacaag agatgacgca gcaggagaag cttatgacaa agttggaaga
    24601 gtattaggtc tttcttaccc aagcggaaaa gaaatcgatc agctagctca tcagggaaaa
    24661 gataattacc attttccgcg agcgatgatc catgaagata attatgattt cagttttagc
    24721 ggattaaaaa gtgcatttat caatttggtg cataatgcac aacagcgtgg agaagatcta
    24781 gacaaaaatg atttagcagc tagctttcaa gcaagtgtga tcgatgtatt gattactaaa
    24841 acacttcgag cttgtcaaaa ttatccagtc aagcaactga ttatagctgg aggtgtagcg
    24901 gccaatcaag ggttgcgtga aggattgcag gatgctttgg caactaaact ccctgaggta
    24961 gaactagtga ttccccctct tcgactttgt ggagataatg ctgcaatgat tggagcagca
    25021 gcacatgtgg aaatgcaaaa aagaaatttt gctagctatc agttgaatgc tgatccaagc
    25081 ttggttttat ctgaataaag aggttgtgaa aaagctgccc tgcatcaagt aataagaaca
    25141 gcccaggaaa aacggcttct catgtttttg acctggctgt tcttattacc gcgtatgcaa
    25201 gcttttgaac gccgtttaat caagagttcg taagcctgtc gtttaactct gattcacaag
    25261 gaacaatttc aaaaaacagc tcctcatgtt tatgaacatt atgacttgtg accgaagagt
    25321 tgacaggtac acaccgttta atcgaaatag ggctgtgaca cgtttttgtc aaagccctat
    25381 tttttacata cttttaatca ttattgttat tttgctattt tttacagctt atttatcatt
    25441 atccgttcct ctgattgccc tcactgagta atcgtttatt gtcgaggaat atcgaataat
    25501 ctagatcgga tgaggacaag acgcttcatc tagttaatct aacagtattt tttgcaaaca
    25561 gtgaatatta cataactgaa acacacatag aaaatctttg agtcttgtcc agtgatctaa
    25621 aactgccgga tctttcagaa gttttaggga ggaaaataag cagtgaccct ga
//
LOCUS       DACDIG010000038        25322 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.038, whole genome shotgun sequence.
ACCESSION   DACDIG010000038 DACDIG010000000
VERSION     DACDIG010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.038"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..2093
                     /gene="acm"
                     /locus_tag="IV247_001622"
     CDS             <1..2093
                     /gene="acm"
                     /locus_tag="IV247_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAP8777694.1"
                     /translation="VYADAGRDISSNVTSLTVDPTNITDGGNIKVKFSFDEKKQNIQP
                     GDYLWINWPSEGNIRGEGFQKEIPLMIENKNVGTLTVRKDSAQVVFNENIKNLDSVEG
                     WGQFEIQARNVTDTGEENTGPFTVTSGDKTATVNVTKPASGSSSSVFYYKTGDMLPED
                     TKHIRWFLNINNNGTYVEQPVKISDEIQSGQRLDPSTFEINQIHLGEQKVYRGEEGIQ
                     QFLQDFPSATFNFSVTDNYIEITIPKNFVNLRKIMVSYKTIIENPEQINFENHSEAWF
                     KEFNKPAVDGESFNHTVKNISASGGVNGTVRGELKIFKYINDTEIGIPNVTFELRRAD
                     EQPIQGQSSILLTSNEQGEASIKGLQVGDYVVKEKEAPNWIDFDPLSSNELKFSINEN
                     DTEGVSLPIYNKKKVTNITATKIWNGGTTPRPSIYFKLFRASTNNWEPVPDAETKRLD
                     NGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGNDYVPSGYQKIENGLVVTNENKEVIS
                     VSGQKTWEDNENQDGKRPTTITVNLLADGQPIQHKEVSEKDDWRYRFTNLPKYRDGQE
                     IIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNISIPGSHITPWKNFPEKTEREKIRNL
                     FSQKLQIFAHTGESSEQRSNYISKRAVPKQITNKSKSILPKTSSKKSSFAVIIGLLIV
                     TICAGIFLQKYKKV"
     gene            complement(2365..2850)
                     /locus_tag="IV247_001623"
     CDS             complement(2365..2850)
                     /locus_tag="IV247_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAP8777695.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            3330..3971
                     /locus_tag="IV247_001624"
     CDS             3330..3971
                     /locus_tag="IV247_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP8777696.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            3990..4625
                     /locus_tag="IV247_001625"
     CDS             3990..4625
                     /locus_tag="IV247_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP8777697.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4643..5458
                     /locus_tag="IV247_001626"
     CDS             4643..5458
                     /locus_tag="IV247_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8777698.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            5777..7213
                     /locus_tag="IV247_001627"
     CDS             5777..7213
                     /locus_tag="IV247_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8777699.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7402..9849
                     /locus_tag="IV247_001628"
     CDS             7402..9849
                     /locus_tag="IV247_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP8777700.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10079..11146
                     /gene="rlmN"
                     /locus_tag="IV247_001629"
     CDS             10079..11146
                     /gene="rlmN"
                     /locus_tag="IV247_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP8777701.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(11339..12442)
                     /locus_tag="IV247_001630"
     CDS             complement(11339..12442)
                     /locus_tag="IV247_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8777702.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            12995..13543
                     /gene="aac(6')"
                     /locus_tag="IV247_001631"
     CDS             12995..13543
                     /gene="aac(6')"
                     /locus_tag="IV247_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP8777703.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            14089..15750
                     /locus_tag="IV247_001632"
     CDS             14089..15750
                     /locus_tag="IV247_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8777704.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(15820..16167)
                     /locus_tag="IV247_001633"
     CDS             complement(15820..16167)
                     /locus_tag="IV247_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAP8777705.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            16251..17192
                     /locus_tag="IV247_001634"
     CDS             16251..17192
                     /locus_tag="IV247_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8777706.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            17193..18236
                     /locus_tag="IV247_001635"
     CDS             17193..18236
                     /locus_tag="IV247_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8777707.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            18242..19312
                     /locus_tag="IV247_001636"
     CDS             18242..19312
                     /locus_tag="IV247_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8777708.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            19302..20255
                     /locus_tag="IV247_001637"
     CDS             19302..20255
                     /locus_tag="IV247_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8777709.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            20267..21277
                     /locus_tag="IV247_001638"
     CDS             20267..21277
                     /locus_tag="IV247_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8777710.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(21562..22383)
                     /locus_tag="IV247_001639"
     CDS             complement(21562..22383)
                     /locus_tag="IV247_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP8777711.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(22480..22758)
                     /locus_tag="IV247_001640"
     CDS             complement(22480..22758)
                     /locus_tag="IV247_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777712.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            23026..25035
                     /gene="metG"
                     /locus_tag="IV247_001641"
     CDS             23026..25035
                     /gene="metG"
                     /locus_tag="IV247_001641"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAP8777713.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
BASE COUNT         8557 a         4397 c         5257 g         7111 t
ORIGIN      
        1 atgtctatgc ggatgcaggc agagatatca gcagtaatgt cacttcgttg accgtggacc
       61 caacgaatat tacagatgga ggaaacataa aagtaaaatt ctcgtttgac gaaaaaaaac
      121 aaaatatcca accaggtgat tatctttgga tcaattggcc aagtgaagga aatattcgcg
      181 gtgaaggatt ccaaaaagaa atcccattaa tgattgaaaa taaaaatgtt ggaactttaa
      241 cggtcagaaa agattctgcg caggttgtgt ttaatgaaaa tattaaaaac ctagactcag
      301 tagaaggttg ggggcaattt gagatacagg ccagaaacgt aaccgatacc ggtgaagaaa
      361 atacgggacc attcaccgtg acgagcggtg ataaaacagc tactgtaaat gtgacaaagc
      421 cagcttctgg ttcttcttct agcgtttttt attataaaac aggagacatg ctacctgaag
      481 atactaagca cattcgatgg tttttgaata ttaacaataa cggcacttat gttgaacaac
      541 cagttaaaat cagtgatgaa atccaaagtg gacaaagatt agatccaagt acgtttgaaa
      601 ttaatcaaat acacttaggt gaacagaagg tttatagagg agaagaggga atccaacaat
      661 ttttacaaga tttccccagc gctacattta atttttcagt aactgataat tacattgaaa
      721 taactatacc aaaaaacttt gtcaatctta ggaaaataat ggtgagttat aagacgatca
      781 tagaaaaccc agaacaaata aattttgaga atcattcgga agcttggttt aaagaattta
      841 ataaacctgc tgttgatgga gaaagtttca atcatacagt taaaaatatt tcagcttctg
      901 gtggagtcaa cggtacagtt agaggcgaat tgaagatttt caaatatatc aatgacacag
      961 agattggtat cccaaatgtg actttcgaat taagacgagc agatgaacag cctattcaag
     1021 gacagtctag tatcctgttg acttcgaatg aacagggaga agctagtatc aaaggcctgc
     1081 aagtaggaga ctatgttgta aaagaaaaag aagcacccaa ttggattgat tttgatccac
     1141 taagttcaaa tgaactcaaa ttctccataa acgaaaatga tacagaggga gtcagtttac
     1201 ctatttataa caagaaaaag gttacgaaca tcacagcaac taaaatttgg aacggtggaa
     1261 ccactccaag accgagtatt tattttaaat tattcagagc aagtacaaac aattgggaac
     1321 cagttcctga tgcagaaaca aaacgattag acaatggaat aacttcagtg acttgggaag
     1381 atatccaaca gtatgatgat agtggaaacg aatatacgtt taaagtgcaa gaagtagatc
     1441 aaaacggaaa tgattatgtc ccttcaggtt atcaaaaaat tgaaaacggg ctagtcgtta
     1501 caaatgagaa taaagaagtc atttcagtct caggacaaaa aacatgggaa gacaatgaga
     1561 accaagacgg caaacgaccg acgacgatca cagtaaatct attagccgac ggtcaaccga
     1621 tccaacacaa agaagtaagt gaaaaagatg attggcgcta tcgtttcaca aatctgccaa
     1681 aatacagaga cgggcaagaa atcatctata ctgtaacgga agacaacgtg cccgaataca
     1741 gtacgacgat tgacgggtat aacatcaaaa acagttatac accggaaaaa acaaatatat
     1801 ctataccagg tagccatata acaccgtgga aaaattttcc agagaagaca gagagagaga
     1861 aaattagaaa tctattctct caaaaattgc aaatatttgc tcatacagga gaatccagcg
     1921 aacaaagaag caattatatt agtaagagag ctgtacctaa acagataaca aataaatcaa
     1981 aaagcattct tcctaaaaca agtagtaaaa aatctagttt tgctgtaatc attggtttat
     2041 tgatagtaac tatctgtgct gggatattct tacaaaaata taaaaaagtt tagttagaat
     2101 ataatatttt ttgttaaata tgactgaaat caaaagaata aaattccgat ttataatcga
     2161 aaaaagaagc atttttatat atgcttcttt tttcgtagaa tcaataaata tggatttggc
     2221 taactatgga agaatactcg tattagtttt cagcgttcat actagttgtt acattgtttg
     2281 attggccaaa agaatttctg atatattttg tgctaaataa agaaaaagac gcttgaattg
     2341 ggaaataaga aaactactct atcattacaa attttcataa actggcagat gttctaagta
     2401 aacaagtatc ttttcaaagt cggaaaaatc cataatcaac gtagtggtat tgatatttgg
     2461 atgaaagcct acggtatctt gacgatcaat agcagcatcg atcacgactt ggattttttt
     2521 ctctttgtcg tgcatcaatg ctaatggagt cactgtacct gctggaacgc ctaggaggtc
     2581 aggaagctgt tcttcagaaa caaaagataa gcgcttttct tttaattgtt cagctagttg
     2641 ttttaaatcg gcgtgctttt catcaggcaa gatgacaaga tagatttgtt ttccttttct
     2701 tgatttcaat aatagatttt ttacttgcgg accaggtagg gaaaaaggga catttttgac
     2761 tgacgtaatc gcaggatgat cgactttttg aaaagagata cccagttttt ttagtagttc
     2821 ataagcttct tgttcggttg cacgactcat cgcgcatcac cctccttttt ttattagtgt
     2881 atcataaaaa agagttgaca aatgaaaaat aatcatgtaa attacacgta aagttaaaac
     2941 aaatgacatc gaaaagaaga gtagccgtta ggtgttttta gagagtctct ggttggtgga
     3001 aagaggcagc agttaaccgt gaaacacatc ttggagtcga ttttctgaag cgtatagtag
     3061 gaaaatccgg ggagcccgtt acagctgcgg tcgttgctag atgaccagat aaggctggct
     3121 tttccgtgag gagtcggtaa aaaaaggtgg aaccacgtga ttaacgtcct ttgatcttcg
     3181 gatcaaagga ctttttgttt tcatctgaac ggcaacgact gtgtgaggtt ttttctgcaa
     3241 ggaaaaaata aacagaggtg gaaccacgta taatacgtcc tcttggcatt tgccaagagg
     3301 atttttttat tttaaaggag gaaaagaaga tggagtattt gattgaagtt atgccgcaat
     3361 tattagtagg agcactcaca acattgaaat tatttttatt taccttgatt ggctcattac
     3421 cattagggat tgttttagca ttaggattgt ctttgcattt taagccgcta cgctacttgc
     3481 ttcaaacata tgtatgggtt atgcggggaa caccgctgtt gttgcaactg atttttattt
     3541 tttatgggtt gccgacgatt gggatcgttt ttgatcgttt cgaagcagca ctcattgcct
     3601 ttatcttaaa ctatgcagct tattttgcag agatctttcg tggagggata caatcgattc
     3661 ctcaaggtca atatgaagca gcgcaagttt tgcaattaag tccctttcaa acggtgacga
     3721 aaatcgtcat cccacaagtg acaaagattg ttttaccttc tgtagggaat gaagttatca
     3781 acttggtcaa agattcttcc ttgatctata tattaggttt aggtgacgta atgcgtgctg
     3841 ggaaaatcgc gatggaacgc gacgcgactt tactgccttt gctaggcgtg gcaatcatct
     3901 atcttacatt gactggtatt ctaacaatca taatgaaaca agttgaagga aaattaaatt
     3961 attaccgcta actgatagga ggaagaaata tgattgaatt gaagaaagtg aacaaagcat
     4021 ttggtgaaaa aaaaataatc aaagatttga atctaatcat tccagatggg aaaacattag
     4081 ctatcgtagg tccttccggt ggcggtaaaa ccacgctttt acgtatcttg gctggcttag
     4141 aaagttctga cagcggagaa ttttatttag acggtgtacc gttcaaccca ttcgagctta
     4201 aaatgaaaga acaagttgta ggagtcgtat tccaagactt ccagctattt ccacatctga
     4261 atatttttga aaacattacc ttagcaccaa aaatggtatt gaaacagaca aaagaagaat
     4321 acaccaaaaa agcagaagaa ttagcggctt cattagggat tagtgattta ttgaatatct
     4381 atccttatca gctttctggc ggacaaaaac agcgggtagc gattgccaga gcattggcta
     4441 tgaatccacg ggtattggct tatgatgaac caacaagtgc tttagatcct gaattacgcc
     4501 agcaagtcga gcgtttgatc ctatcattga aagaagatgg ggtaacccaa attattgtca
     4561 cccatgattt agtatttgca gaaaatgtcg gtgatcaatt actaaaagtt tccccaattc
     4621 attaagaaag gagaagatag agatgaaaaa aaccagtttt ctattcgctt tgatcgcagg
     4681 tcttcttctt tttgcaggct gttcgaacaa aaaaacttca gcagataatt gggcgactat
     4741 caacgaagag aaaaaaatcg ttataggttt agacgataca tttgttccta tgggatttag
     4801 agataaagac gggaagctca caggatttga tattgattta gccaaagcag tctttgaaca
     4861 atatgggatt acggtagact ttcagcccat tgactggtca atgaaggaat ttgaactgaa
     4921 caatggaacg atcgatttga tatggaacgg ctattccaaa acgccggcaa gagaaaagaa
     4981 agtgcaattt acaaaacctt atatggaaaa cgatcaagtg ctgatcacac ctaaagaatc
     5041 aggaatcaca aactttggac aaatgaataa taagagatta ggtgcgcaaa atgggtcatc
     5101 tggttatgat gtatttacga aacaaccaga agtattaaaa gagatagtca aaaaccaaga
     5161 tgctgttttg tatgatagtt ttaatgaagc attgatcgat ctgaaaagca gacgggtcga
     5221 tggtctgctg atggataaag tatacgcaga ctattatttg aaacaaagaa acgaactaga
     5281 agacttcaac ataacaaaag gttcttatga gagtgaggat tttgcagttg gtgcacgcaa
     5341 gtcagatagt acattagtga aaaaaatcaa tgaagcccta gatcaactag agaaaacagg
     5401 tgaattccaa aaaatatccg ataaatggtt tggcgaagat gttacgccga gaaattaaca
     5461 aataggggac tgtgacaaaa gacgtgtcac agtccctttt tccgaataaa cagtgttctc
     5521 ctgccaactc cttgtgactc ggagctgaat ggcaggctca caacctcttg gtaaacggtg
     5581 ttcaaaagcc agtttttcgg tattaagcca aatttatgca aaaatgtaga aagccatttt
     5641 cacaaaattt ttcttactac tcgaaactaa gcgacttttt cacaacctcc tttacataat
     5701 tttattttgt tgtggaaggg gtttcgtgtt attctaaaag aggaacgatt gaaggttata
     5761 ggaaaggaag agatgaatgg aacacaaaga acttacctca tttccaaaag attttctgtg
     5821 gggctctgct tctgcagcgt atcaagtaga aggagcttgg caagaagatg ggaaaggaga
     5881 atcagtctgg gatcgatttg tacggatacc tggaaagaca tttaaaggaa cgaacggtga
     5941 tttggcagta gaccactatc atcgttataa agaagatatt gctttaatga aagaacaagg
     6001 tctgaaagct taccgcttct ctgtagcttg gacacggatt tttcctaatg gacgaggaga
     6061 agtcaatcaa gctgggctcg ctttttatga gagattgatc gacgaattga ttgaaaacga
     6121 tattgaacct atgttgactt tatatcattg ggatcttcca caagcattgc aagatgaata
     6181 taatggttgg gaatcacgtc agatcattga agatttcact cattatgcag aaacattatt
     6241 tgaggcattc cgcgggaaag tccattactg gattagtttg aatgaacaaa atatcttcac
     6301 ttcattaggt tatttgttag cagcacatcc gccaggtgtg acagacccga aacgaatgta
     6361 tgaagttaac catattgcta atttagcaaa tgcatctgtc atcaataaat ttcacgagtt
     6421 agaaatgcct ggaaaaatcg gaccaagctt tgcgtatacg ccaaattatc caattgacag
     6481 taatcctgaa aatgttctgg ctgctgaaaa tgcagaagac ctgatggcca attattggat
     6541 ggatgtctat atgtggggaa aatatccgat tgctgccatg aggttcttag aagaaaaagg
     6601 atgggcacca acgattgaag ctggcgatgc agaattgcta gaatctgcta aaccagattt
     6661 tcttgggatc aattactatc aaacggcgac gaatgcattc aatccattaa acggagttgg
     6721 tgctggaaaa atgaatacga caggaaaaaa aggttcatca gaagaaactg gagttcctgg
     6781 aatgtataaa aaagttgaga atccatttgt cgaacgaaca aattgggact gggagattga
     6841 cccagaagga ttaaggatcg gactgcgtcg tatcacaagc cgttaccgca tgccggtact
     6901 gattacggaa aatggcttag gagagtatga caagttgaca gaagataaac aaatccatga
     6961 cgactatcga atcaattact tacaaagcca cgtcaaagcc atcaaagaag caatttcgga
     7021 tggagcagaa gtcctaggct attgcacctg gtcttataca gatttgctta gctggctgaa
     7081 cggttatcaa aagcgatatg gatttgtata tgttgatcaa gacgaaacac aagaaggatc
     7141 tttggaacga tataagaaag acagttatta ctggtaccaa aaactgattg aagaaaatgg
     7201 agagaacctt tagttcacat acttaaaaca agaaggtacg gctggatatg gccgtgcctt
     7261 ttcttgataa aatcatttag aaattaagaa aaactgtaaa ataagctgaa aaatgaaaga
     7321 aagcgtcagt tctttcaaac aaaactttag ttcccttact tttttttgta taatgaaact
     7381 tgatgaaatg agggataata aatggcacaa aagaaacggc ctactaaaaa aaggaaaaag
     7441 acgaaaaaaa cgaaaaagca aaaacagcag caggaacaaa tgctgacgat tggtgttggg
     7501 tttgccttga ttttatttgc tgtcttcggc tttttaaaat taggtttttt aggaatattg
     7561 attgcaaacg gctttcggat cattgcagga aatacttacc aaatcctctg tcttttatta
     7621 gccgtattag gattttggat tgtcattaag aatacagagt tttcaattgg taaaaaccga
     7681 cgctggtttg gcggcatact cttttatttc ggtatcttat tattgttaca tgcccattta
     7741 tttggcaaac tccatactgg ggaaccaaat atcatgggga cgacatggga tttgttagcc
     7801 agtgatataa aacaaagtca agtagacaat aacgttggtg gtgggatgat tggagcaatc
     7861 ctttatcatt ttacttactt cttgattgca cagcctggaa gttatttagc agcagtgcta
     7921 ttactggcag gaggagcatt cctgttcagt aatctggaag gctatcagct gttaaatggt
     7981 atccaatcaa ttggagaacg agtacaggaa ttattggaag gtgatcctgc caagcaagct
     8041 cgaaagcaag cggcaaagga agagagaatg aaacagcgcg cagaagcgaa agaagcaagg
     8101 cgcttagctg caaaagaagc tgcagaaaaa gaagctgcag aatatgagaa gaaaaaagcg
     8161 gtgagccagc aaagggaaaa tactgctgat gaatggcagg aaccagaaaa tacagagccg
     8221 gagcagctaa gttttgttcc aatcgacagt tttcaagaaa atatccatcc tgcgaatcta
     8281 gagaaaccag taccagatac accaaaacag accaatacag cagaaggttt ttcagatgag
     8341 ttgccagagg atgacggtac ctcattagaa tttgagattg aagcagaaca ggaaaatcaa
     8401 gactatgaat taccgacagt tgatttgctt gattctatac caactgtgga tcaaagtgat
     8461 gagtataaaa aaattgaaaa aaatatcggc gtattagaac aaactttcca aagtttcggt
     8521 gtcgatgcca aggtcgtcaa agccagctta ggaccatccg tcacaaaatt cgaagtccag
     8581 ccggctgtag gggtaaaagt aagtaaaatt gtgaacttaa cagatgatat cgcgcttgct
     8641 ttagcggcga aagatgtccg gatggaagca ccgattccag gtaaatcact gattggtatt
     8701 gaagtaccaa atggaaagat cagtatggtt tcctttagag aaatcatcga agcacaacca
     8761 aaccacccag ataaattact agaagtacct ttaggacgtg atgtttctgg acgggtacaa
     8821 acggctgatc tttcgaagat gcctcactta ctcgtggctg gttcgactgg tagtgggaag
     8881 tcagtagcaa ttaatgggat tatcacaagt atattaatgc gtgctaagcc ccatgaagta
     8941 aaactgatga tgatcgatcc gaaaatggtt gaattgaata tgtacaacgg catacctcat
     9001 ctgttgacgc cggtcgtgac gaaccctaga aaagcagcgc aagccttaca aaaagtagta
     9061 caagaaatgg aagaacgata tgaaaaattc gcggcaaccg gagtaagaaa tatttctggt
     9121 tacaatgaat ttgttcaaca gaaaaacttg gaaaacggaa cgaaacatcc aaccttacca
     9181 ttcatcgtag tgatcgtaga tgaattagct gatttgatga tggtagccag caatgaagtt
     9241 gaagatgcga ttattcgttt agcgcaaatg gctcgggcag cggggattca tatgatccta
     9301 gctactcaac gtccaagtgt ggatgttatc acaggaatca tcaaagcaaa tgtaccttcc
     9361 agaatggcat ttgcggtatc aagcggtaca gattctcgga cgatcattga ttctaatggg
     9421 gcagaaaagc tcttagggcg aggagatatg ctattcttgc caatgggtga gaataaaccg
     9481 atccgtgtgc aaggagcatt catttctgac cacgaagtag aacgggtcgt tcaatttgtt
     9541 acagatcagc aagaagctca ttatgaagag aaaatgatgc cgacagatga agttgaaacg
     9601 acaggtgctc cagaacagcc acaagatgag ctgtttgaag aagcaaaagc acttgtcgtt
     9661 gaaatgcaaa cggcaagtat ttcccttctc caaagaagat ttcggatcgg ttacaaccga
     9721 gcagcacgtt tagtagatga actggaagct catggagttg ttggcccgtc agaaggaagc
     9781 aagcctcgga aagtattcat tgagcaggaa gaaacatcag aaacgttgcc ggacgataat
     9841 gacatgtaaa acttgtgtga tgaagggttg aaaaacgaat aaccaataaa aagacaaggc
     9901 atgatgatac caaaagaaaa aatggttttt ttccatcttt tctaatacgg tatgatgtct
     9961 tgtcttttta gtttttgtga gattaaagtt ctattcgatt tatttgtgaa gtattgcatt
    10021 aaggaaatga atcatgtata gtatgtagcg aaactttgag aaaaagaggg aaaatacttt
    10081 ggaaaaacag tccatttatg gcttgacgaa tgaagaatta atcaattggt ttatagaaaa
    10141 cggcgagaaa aaattccgag ctgcccaagt ttgggaatgg ttgtatcaaa aacgtgtaag
    10201 taatttcaca gaaatgacga atctatcgaa acaattgatc gaaaaattat cggagcattt
    10261 tatcatcaat cctctaaaac agatggtcgt ccaagaagct tcggatggta cagtaaaata
    10321 tttgtttgaa ctccctgata aaaacatgat tgagactgta ctgatgagac aagaatatgg
    10381 cttgtcagtt tgtgtgacga cacaagtagg atgtaatatc ggctgtacgt tttgtgcaag
    10441 cgggctgctc aaaaagaacc gtgatctgac tgctggagaa attgttgcac agatcatgat
    10501 ggtccaacat tattttgatg agcgcaatct tggcgaacgc gtttcacatg tcgttgtaat
    10561 ggggatcggt gagccatttg ataattacga caatgtaatg gactttttgc atatcatcaa
    10621 tgatgccaaa ggattggcaa ttggtgctcg gcacattaca gtttccacta gtggattagc
    10681 acacaaaatc aaggaattcg ctaataacgg cttgcaagtc aatctggcta tttccttgca
    10741 cgcgccgaat aatgaggtgc gaacatcgat catgcggatc aaccgaagtt tcccaattga
    10801 gaaattgatg gaagctgtgg atgagtattt agagaaaaca aaccgtcgaa tcacgtttga
    10861 atatatcatg ttgaatcaag taaatgatag accagagcat gcgcaacaat tagcagatct
    10921 attaaaagat aagaaaaaat tagcatatgt caacttgatt ccatataatc cagtcagtga
    10981 acatgatcaa tattctcgca gtccaaaagc tgatgtattg agattttatg atgtattgaa
    11041 gaaaaacggc gtaaactgtg tgatacgtaa agaacatggt acagatattg atgcagcttg
    11101 cggacaattg cgaagcaagc aaatgaaaaa aacagttgcc aaataatcgt atcctactcg
    11161 ttttttcaat taccataaaa ggagcagttt tcttaattaa ggaactgttc aacttaaata
    11221 agagaaaaga ttctatagcg aaagctataa agcttttttt gacaagaaaa accctcatca
    11281 agcaaagaaa tgtcctcaat ttgatgacag cctgcttgat gggggtttta gcaattattt
    11341 aactgatttt cttatatcct tgctggcttt cttgtgtagc atgataagtt tctacgtcaa
    11401 gaatggcctg tttaaatttt tctcgggtga tttcataagg cacatgttta atatcatttg
    11461 ttgccatggc tttatctaag attgcttcta aatcggtcgt gacattgaga ttcatgtcgg
    11521 ttaacgatac tggaagttgg ttctttttca taaacacata gattttatca aacaaatcga
    11581 actgctgatc catcatcaat aaggtcagta caccataact gacagatgct ccgtgaaggt
    11641 atccctccat tttttctaga acagtcgtac cgtaatagaa tgagtgtgcc atagcagaat
    11701 tatagttatt gttaactaaa acagaaacca atccggtagt aacaatgata tccgaaacga
    11761 cttgttcaat tgcatatgag ggacgatcgt cggcactatc tttcaatgct tgcgtaccat
    11821 aagtcaagag acgatcaatg cacatttttc cgatttggat tcctaatcta tccgtgtagc
    11881 ctagatcatc gccacgagca gaaaaagtag attcgacttc tttggataaa gcatcgccaa
    11941 tacctgccca taagtattta tcaggtgctt cggcaataat tgctgtatca atgaaggtgt
    12001 gtacaggtgg ttttgaagga aagaagagtc cgtccattac gccttcttcg ttatagatga
    12061 cacataccgc agtgacgggc gaacagttcg aaccaatagt gggaaaggtg aagactggtt
    12121 tagctagttt ttcaccaatg acttttacag tatcgcaagc acggccacca ccgactgcaa
    12181 acaggacgtc tgctttttgg acactttcat gctggatcaa ctgttcaata tttccataag
    12241 ttgaattacc cccgtaccag agactggcga caacctctat ttcctgtgtc aaagctactt
    12301 ttattttgtt ttctgccttt gaaagtgcag tcttccctcc aatcagcact acttttgtcc
    12361 catattcttt gacgattcca tttgcttttt catacgcatc tgttccaatg gtataatttg
    12421 gtaagaaaac actttgactc atattcattt ccttctttct tcgttgaaac gggttaaaat
    12481 tttaaagagt gatcgatctg actgcggctg tagatggagt agaggtgaac ctaagacagg
    12541 ataagtgatg aataaggtgg catgaacacc ttgaaatcat caataagtat cctaaaaacg
    12601 aacaagtaca aaaacattga aaacgacaat aatgaatttt tgttcctctt agtatttcta
    12661 ccgattccct ttgctttttt gtaattatct aaaaattcta aaaattaaga ttattatatg
    12721 aaaacaggtt ttatttgtca attcataatt ggtttttttt aaagcaaaag ataatctata
    12781 tcatgatgaa caaacagagt tttttcctcc aattgatggc tttcctgatc tttcctattt
    12841 tgaataaagt gtttctgtat aattttttta ttttataagt aagataaata atgaaagaat
    12901 agagatactg attggtagct aaagattttt gagcgagaac atttcgtttt ttcaaaagga
    12961 ctgttagaat aagcaagaac aagggggaag acgtatgata atcagtgaat ttgaccgtaa
    13021 taatccagta ttgaaagatc agctttctga tttactgaga ctgacttggc cggaagaata
    13081 tggagacagc tcggcagaag aagtagaaga aatgatgaat ccagaacgaa tcgcggtagc
    13141 agcggtagac caagatgagt tagtaggatt tattggtgca atccctcaat acggtatcac
    13201 aggttgggaa ttgcatccat tagttgtaga aagctcccga cgaaagaacc aaataggtac
    13261 tcgattagtc aattacttag aaaaagaagt agcttccaga ggaggaatca cgatttattt
    13321 aggtacggat gatttagacc atggaacaac gttaagtcaa acggacctgt atgagcatac
    13381 atttgataaa gtggcttcca tccagaacct tcgtgaacat ccgtatgaat tctatgaaaa
    13441 attaggttat aaaatcgtag gtgtcttacc aaatgcaaat ggctgggaca aaccggatat
    13501 ttggatggca aaaacgatta ttcctcgacc aaattctcaa taataaaaag aggttgtgac
    13561 agaagtaatc agcttcaaga aataagaagg aatttacgaa aattacttct tgaatttttg
    13621 tgaaattcca acttatttcc gaagaagcta cttttggaac accgtgtacc agaggttgtg
    13681 aaaaagtcgc ttagtttcga gtagtaagaa aaattttgtg aaaatggctt tctacatttt
    13741 tgcataaatt tggcttaata ccgagaaact ggcttttgaa caccgtttat tcagataagg
    13801 gactgtgaca agacttttgt cacagtttct ttttgtttat tcttttttat ctttgtatgg
    13861 caataataga caatataacg aaaattattt aatggctttt ggaagaccat ggaaaacctt
    13921 gtacaaagct attttttctg aaaaattcct tgaaacctca cgaaaaaatc attattttat
    13981 gaaaaagaca aaaaaaagtg ttgatttttt aataattatt cattaaactc ttaaacaact
    14041 tcagaaaata ttaattattc tgaaaattcc aataaagggg tacgtaaaat gaaaaagaaa
    14101 tttacatttg gtttggttgc ggtatgcggc cttgtacttg ctggatgtta tggcggcagt
    14161 tcagccacaa gcagtagttc agcaaaaagc ggttcagcag atggaggagg cgtatttaac
    14221 ttagtcgtgc ctcaagaaat gcctacagct gacttatcag ttgcaacaga tacaatcagt
    14281 tttacagcat tgaataacgt ttatgaagga atttaccgtc ttgatgaaga cagcaaacca
    14341 cagccggcag gagctagcga gttggcagaa gtcagtgaag acggattaac ttacaaaatc
    14401 aaattacgag aagacgcaaa atggtcaaat ggtgatcctg ttacagcagc ggattacgtt
    14461 tatggctggc aacgaacagt agacccagct acagcttctg aatatgctta cctgtatgcg
    14521 ccagttgaaa atgcagaaga cattacagct ggaaagaaag ataaatcaga actagggatc
    14581 aaagcagttg gtgactatga attagaaatc aaattgacga aacaaacacc atatttccaa
    14641 tatctgcttg ctttcccatc atttttccca caaaaacaat ccgtagtgga agaaaatggg
    14701 gatgcctatg catctgcaag tgataaagct gtttataatg gaccattcac attagaagat
    14761 tttgatggac caggaacaga tacagaatgg acttataaga aaaatgatca ttactgggac
    14821 aaagatacag taaaactttc tgaaatcaaa gtcagtgtag taaaagaatc ttctactgca
    14881 ttgaacttat tcaaagatgg ccaagcagat gacgtgatct tgtctggtga gttagcacag
    14941 caaaacgcaa acgatccagc ttatacttct gttaaagaag cgcggacaag ctatatcgaa
    15001 ttcaatcaac gtgaaaagaa ttcaccattc aacaatgtga acctaagaaa agctatttct
    15061 tattctatcg atcgtgaagc tttagtaaaa caagtactag gtgacggatc agttgtctct
    15121 acaggattga tcccatcaga tatggtaaaa agtccaacaa cgaacgaaga ctttgcaaaa
    15181 gaagctggaa aattagtttc ttatgacaaa gataaagcaa aagaatattg ggaaaaagcg
    15241 aaaaaagaat tgggcgttga ttctttagaa tttgatttga tggcttctga tgatgattca
    15301 tcgaaaaaag taatcgaata tgttcaaaac tcaatccaag aaaacttgga tggcgtaaca
    15361 gtcaaaccaa cacctgttcc attctctgta cgtcttgacc gctcaacttc gggtgatttt
    15421 gacgcagtac taggcggatg ggctgctgac tatgcagacc caagcagctt tactgatttg
    15481 tttgtcacag gtaattcata caaccgtggt cgctggagca atgcagaata tgacgaagca
    15541 gttaaagcag cttcatcaac agacgctggc gatgaacaag cccgttggga tgatttccaa
    15601 aaagcaatca agatcatctc tgatgatatg ggtgtcgttc cggtgtacca aaaagcagaa
    15661 ggtcacctag tgaatgaaaa aatcaaaggt atcgttcacc atgcggcagg cgcttcatgg
    15721 gattacaaat ggacatacgt tgaagaataa tcgttaatac cgtgagcatg agaaaaaacg
    15781 aggctgcggc aaatgtcgca acctcgttct catattcact tatcctaaca aagaaaaaag
    15841 catgaaaata gcagaaagag caattagtat tccggcaatg atcagccaaa agctgtacat
    15901 accgtagctg gcagacgaga gcgaggaaaa ttctggtttt ttctttcggt tccttgttcg
    15961 tgcaagatgg accgatcgct gaaaatgatc gaagaaaccc gaagaaaaga cccataaaaa
    16021 accgccgata atcagaaacg gcaatgacca taagaataga tcattagata attgaatgag
    16081 tgtaaaggaa tggttgatga gattttttat taaaataacc agaatcagta gaaaagctac
    16141 tgcgtagggg atagattttt ttttcatttg attgctcctt tatttttggt tcattatcta
    16201 ataatgtagc gaaacatctg tcagaagtca aaagctaggg ggaaacatta gtgaatagtt
    16261 atatcaaata tgtattaaaa cgtgtctttt tcatgatcat cacgctttgg ttaattgcaa
    16321 caatcacctt tttcttgatg caattgctgc cgggcacgcc atatacgaat caggaacgtt
    16381 tgagtcctga aacaatcgag atgctaaata aacaagtcgg tttggataaa ccagttatcg
    16441 tacaatatgg gatttatctt tctaatttgc ttcaaggaga ttttggaatt tccttccaat
    16501 ttaaaaacca gccagtcgca catttgttag ctggaagaat cggaccatcc ttgcagttag
    16561 ggttacaggc aattatcttt ggaacagtcc tagggacgat tttaggaacg atctctgcca
    16621 tgaagcaaaa tacatgggca gatacatctt ctacactcgt agcgatatta ggtcgttcca
    16681 ttccaaactt tgtatttgct gttttattac aatatatctt tgcaatcaaa ttacaagttt
    16741 tgccaatcgc aaaatgggat ggctttgtgt acacaatctt accaacaatt gcgttggcca
    16801 tgtcgccgct tgctgactct gctcggttta tccgtacgga aatggtggaa gtactgcata
    16861 gtgattatgt ggaattagca aaagcaaaag gattgagccg ttgggaaatc gcctttaaac
    16921 atggtcttcg aaatagtttg attccattga tgactttatt aggaccgtta gctgtagcat
    16981 tgatgaccgg atcactggtg gtagaaaata tctttgctat tcctggtatc ggggaacaat
    17041 tcgttaagtc aatcacaaca aatgattatc ctacgatcat ggctgtaacg atcctttact
    17101 catttatgct gatttttgtt attttgatcg tggatctatt gtacggtcta gttgatccaa
    17161 gaattcgtgt ttcagaaggg agccgaggat aaatggaaat gataccaaag cgttatgaaa
    17221 cagttgcaga tatccctgcg gatgaatttc ttccattaca aaaaaatacc gaagaagaac
    17281 gtgaacagat tactgcacct tctttgaatt ttatccaaga ttcatggcgc cgtttaaaga
    17341 aaaacaaagc tgccgttatt tcaatggttt tactattggt catcattttg atctctgtga
    17401 tcacaatctt tgtttctcct catgatccaa ctgcccaaaa cgtggcttat atcaatttgc
    17461 ctcctcgtat tccgggaatc aatatcgatg gattgaatgg aaaagcaatg gttggtggtg
    17521 aacttgttga taaatatgct caagcaaacg tcccttcaaa cgtaaatttc tttttaggaa
    17581 cagacggttt gggacgtgat gtattaagcc gcttatttat gggaatgaga atctcgttgc
    17641 tgatcgcatt tattgctgca ttactagatg taacgattgg tgtagcttac gggttggttt
    17701 ctggtttgtt aggcggacgt gtcgataacg tgatgcaacg ttttttggaa atcttatcag
    17761 gaattccaaa cctcgttgtc atgattttga tgttggtcgt ttttgaacca ggaatttttt
    17821 caatcgtggc tgctatggcc atcacaaact ggataccaat ggcacgtatc gttcgtgcac
    17881 aaacaatgaa attaaaagat caagaatatg ttttagctgg attgacatta ggtgaatcaa
    17941 gaatgaaaat tgcattcaaa catattttgc cgaatatctc tagtgtcatt attattcaaa
    18001 tgatgtttag tattccatct gctattttct tcgaagcctt tttaagcttt atcggtttag
    18061 gtcttacgcc tccgtcagct tcattaggaa cgatgttgag cgaagggtac aagacattcc
    18121 tgtatctgcc ttatctatta tggattccgg cagtaacttt atcggttatc atgattggct
    18181 ttaacttact tgctgatgga ttgcgtgatg cctttgatcc taaaatgaaa gagtgaagac
    18241 gatgacgaaa attttagaag tgaaagactt agaaatctct tttgatacgt atgctggaaa
    18301 ggtacgagcg attcgcggtg tcaattttca tttgaataaa ggagaaacat tggcgatcgt
    18361 tggggaatct ggaagtggaa aatcagtgac gaccagaagc atcatgcgct tgctgtcaag
    18421 caacgccaat atagatgctg gtcaaatcct ctttaaaggt aacgatatcg ttggaaaatc
    18481 agaaagagag atgcaaaaaa tccgtggtaa agaaatagcg atgattttcc aagatccaat
    18541 gacctcttta gatccgacga tgcccattgg gaagcaagtc gctgagtccc ttagaaagca
    18601 taataaagtt tcaaaaaaag agggacaaaa agctgcatta gaattgctta aattagttgg
    18661 aatacccgaa gctgagaaac gtatcaacag ttatccgcat cagttctcag gaggtcagcg
    18721 tcaacggatc gtgatcgcga ttgcactggt atgttatccg gaaatcctga ttgctgatga
    18781 acctacaaca gcgcttgatg tgactattca agcacagatt ttggagctgc tgaaagaaat
    18841 tcagacaaaa atcgatacat caattatctt tatcactcat gaccttggtg tagtagcgaa
    18901 tgtcgctgat cgtgtcgctg tcatgtatgg aggaagaatc gttgaagttg gaacatcgga
    18961 agaaattttc tacaacccgc aacatcccta tacttgggga ctgcttggtt caatgccgac
    19021 attagatagt gcgaacgatc gattgtatgc tattcctggt tctccaccgg atttattgaa
    19081 cccgccaaaa ggagacgcat tctatccaag aaatgaattc gcgatgaaaa tcgatgcaga
    19141 acaagaacca ccattttttg aactatcaaa aacacacaag gcagcaactt ggctacttgc
    19201 tccgcaggca cccaaagtga caccgccaga agaaatccaa cgtcgctggg ctatctttaa
    19261 agagaaacaa aaacatactt acggtcaagg aggaattgct gatgccaaat aaactacttg
    19321 aagttaaagg attgaaacag tatttcaatg taggaaaaaa gaatgaagta catgctgtaa
    19381 atgacatcag ctttcacatt tatgaaggag aaacattcgg tttagttgga gaatctggaa
    19441 gcggaaaatc cactactggc cgtacgatca ttcgcttgaa tgaacctact gatggtgaga
    19501 tattattcga cggtcaagat ataacgaaga tcaaagataa aaaaggtttg acgaaattcc
    19561 ggcatgatgt tcagatgatc tttcaagatc catacgcttc gttgaatcct cgtatgaaag
    19621 tcagagatat cattgccgaa ggaattgatg ttaatggact agctaaatca tctgaagagc
    19681 gtgcaaaaaa agtcgatgat ctattgagaa ctgttggatt gaatccaagc cacggtactc
    19741 gttatcctca tgaattttca ggtgggcaac gccagcgtat tggtattgca cgagcattgg
    19801 cagtaaaacc gcgttttatc atttgtgatg aaccaatttc tgcactagat gtgtcgatcc
    19861 aagcccaagt ggtcaatttg ttgcaagatc tccaaaaaga acatcaattg acctatctat
    19921 ttattgcaca tgatttgtcg atggttaaac atattagtga ccgaatcggt gtaatgcata
    19981 acggcttgtt attagagatg ggaacaagtg atgaaatcta taatcacggt gttcatcctt
    20041 atacagaaag tttgttatct gctattcctc taccagatcc agaccatgag agaaaacgca
    20101 gaagaatcaa atatcaacct gaaccagatg acggacaagt acgtcaactt cgtgaaatcg
    20161 caccagaaca ttttgtctat gcaactgagc aggaagtccc atattacgca aaaaaactaa
    20221 aacgacaaaa ggaatctctt ctggttgcta actaggaggg acatagatgc taaaagcttt
    20281 taaattccgt atgtatccga cagaagaaca aaaacagcaa ttgattcgta cttttggatg
    20341 tgctcgattt acctataacc atttgctgaa aaagcggcag aaaagttggc aacaaacagg
    20401 agtagccaat ttttcgctca cacccgctac attaaaaaaa gaatacccat tcttaaaaga
    20461 agtagacagt cttgctttag caaatgcaca gcttaatttg gatcgggctt ttcgcaatta
    20521 tttcaaggga cgtgccagct tcccaaagct taaaacgaaa aaaagtatgt ggcaatctta
    20581 tacgaccaac aaccaaaccc gtacgatcta tctggaaaat ggctacttaa agttaccaaa
    20641 acaaaaagaa ctaatcaaga taaatcagca tcggccagtt gaaggatcga ttcgctccgc
    20701 tacgatttct gctcgatata atgaagaatt ttatgtcgca ctactttgtg atgtttcgcc
    20761 agtaaaaaaa gaatctttag ctaaatggat cgggatcgcc tatcatccga agacattgat
    20821 ccaaacatct cgacctctag aagtaacttt accaaaattt caccagacag aagaaaagct
    20881 gcaacatgca cagcggaaat tgaatgtgaa agtccgctcc gctcaccata gaaagatcag
    20941 gttagatcag gcaagcaatt atcagaaaca aaagagaaaa gtaatggatc tttatctgaa
    21001 acaaaagaac cagcgagaag attatcttga acaattatct gggaaattgg tcaaacaata
    21061 tgactactta tttgtagaat cttttccaaa agaagaggca catgctgatt tttctattca
    21121 cgactggcat aaattaataa caaaattgcg ttataagtcc caatggtata ataaaaaatt
    21181 tcttttgatc aataccgacg gagcagaaga aagcaattcc gtaagaaaga gccaagtagt
    21241 agaaaaaatg ggcagacatt cggttatcaa gggataaaaa atgaatgaaa ctgataggag
    21301 cgttagggtt agccttgtta ataaaaagtc ttaatgactt ttgtttctag gaatttttct
    21361 tttgaagaaa agctcactaa ttatgacaaa ccaataaaac aatagcagaa aaagttcgta
    21421 cgagcttttt ctgctattgt taatttttta ttcagaaagt ttacgaatac atcaaaattc
    21481 tgaaacaaaa aacgtaactc acaaagagtt gtttttgcgt tttttcttga atgattaggt
    21541 ctgaatcttt tgctcacaac cttattcatg gtgccccggc ttgagctgat agatttttga
    21601 aggacggccg attcctacag gacgttcacc gatctcatcg aaatattgga gcatagcttt
    21661 cttaaaattg gaatgatcga tcgttttata atcgatccct aagaatttag caaatacttt
    21721 tcgagcttcc gtaatcgtga aatctttacc taaaacttgt agaacttgcg gctcgtgttc
    21781 cattttattg actacacggt tgaatgcttt caagatgatt tgactatggt caaaagccaa
    21841 agtatctttg ccagaagact ctccagtgac taaatcaagc gagatagcca cttctccgct
    21901 agataaatgg atcttgttac tatgacgttc tagtgtgaac cagcgtactt ccttggcatc
    21961 atctccggcg attagcggtt cttctccaat aaaggctaag tagctgactg tgacgaccca
    22021 gcctcgcgga tctcgattag gcgtactgaa tgtatgcagt tgctcaatat tttcttttga
    22081 aataaccaca ccagtttctt cctttgtttc tcttaagacg ctttctccag ttgactcatc
    22141 cttttggaca aagccaccag gcaatgccca tgaatttcga taaggatgcc cttttctttg
    22201 gatcaacaaa actttcagct gatcttcttc tttgttatag cacatcagta cgatatcgac
    22261 agtcaaagag ggcttgtcat attctggacg ttcctgtttt tgataccagt taagaaattc
    22321 ttcttcactt gctttatgtt cataatataa tttttcttct tctttagaag caaaacggtt
    22381 catctgacca cctccattga cagtaaaagt actttttacc ttattgtagc agactctgaa
    22441 aaaaaaccaa ggatttactt gaaaaatcta ggttatcatt taaatatgaa aaataaatgg
    22501 aagtatgaga ttgaataaaa agaaaagcag ataaaacggc aatactttat accgctcttc
    22561 acgaatggca aagaagccac aaataacgat atatacgtca atgataaaac gcaaaatcca
    22621 agctgctgtc ataaaactgt tcaaatgccc atggaggtta aaataaccat acatacgaat
    22681 ataaaaatag ctgaatatta aatgtgctaa tgaaagaaaa gcaatactaa gtgtaaaaaa
    22741 aagatgtttc ttctgcatga ttgttccagt cccttactaa gatagatagt ctttattata
    22801 ccacgtgtga ggggagaaat aaaaagccgt tttttagtag atatgaaaca aaacaaaaac
    22861 ccgcatttat tcaacctatt catcctttta aaggatagat tgaacgtaga tagaagtcgt
    22921 ttatccagtc tttttcattt tgaaaaaaag ctaacagcat gcttttttta tggtatacta
    22981 gcaaggaaac gaattcgcat catgcgataa aggaggaaga gccccgtggc agaaaaggaa
    23041 actttttata tcactacgcc aatctattat ccaagcggca aacttcatat cggcaactcg
    23101 tatactacga ttgcatgtga cgcaattgct cgttataaac ggttgatggg ctttgatgtg
    23161 ttttatctca ctggagtgga tgaacatggt caaaaaatcg aaacaaaagc agaagaacta
    23221 ggtgtaaaac cgcaagaata tgtagataaa atggcggctg atgtcaagaa attatggaaa
    23281 acgcttgata tcagttatga taaattcatt cgcacgacag atgattacca taaagcagct
    23341 gttcaaaaaa tatttgatcg tttattagaa cagggagata tttacttagg tgaatatgaa
    23401 ggctggtact ctgtttctga cgaagaattc tttacagaaa cacaacttgc agaagtgtac
    23461 aaagatgacg aaggtaacgt gatcggtggt aaagcgccaa gtggacacga agtagaattg
    23521 gtaaaagaag aatcatactt cttccgcatg agcaaatacg cagaccgtct attggaatac
    23581 tatgaagaac atcccgaatt catccagcca gaatctcgta aaaacgaaat gatcaacaac
    23641 tttatcaaac caggattaga ggatcttgca gtttcacgaa caacttttac ttggggagta
    23701 ccagtcaaga gtgatccgaa acatgtggtg tatgtatgga tcgatgcatt atccaattat
    23761 attactgcac taggatatgg ctccgaagat gaaagtctat tcgaaaaata ctggcctgca
    23821 gatgttcaga tggtcggtaa agaaatcgtt cgctttcata cgatctattg gccaatcatg
    23881 ctgatggccc ttgatttgcc tctacctaaa aaagtatttg ggcatggctg gctattgatg
    23941 aaagacggaa aaatgtctaa atcaaaagga aatgtcgttt atcctgaaat gcttgtggaa
    24001 cgttatggtt tagatgcact ccgttattat ttgcttcgtg ctgttccgtt tgggtcagat
    24061 ggtgtcttta cgccagaaga ttttgtttcc cgtgtcaatt atgatctggc aaatgatcta
    24121 gggaacttat tgaatcgaac gattgctatg atcaacaaat attgtgatgg actcgttcct
    24181 gactatgcct cacttgtcac tccatttgat agtgaactgt ctacgacggc tgctaacgtt
    24241 gtcggacgtt accatgatgc aatggaaaaa atggaattca atacagcatt ggctgaaatc
    24301 tgggtgctga tttctcgtgc aaacaaatac attgatgaaa cagaaccatg ggtattagca
    24361 aaagacgaag aaaagaaagc tgaattgaat agtgtcatga tccatttagc tgaatctttg
    24421 cgtatcgcag cgatattact tcagccaatc atgacagaaa cgccagagaa gatcttcaat
    24481 caactaggat tggactctga aacaatgaat cttgaaggat tacactttgg cgaatttcct
    24541 tcaggaacaa aagtagttgc aaaaggtaca ccaatcttcc cacgtttaga tatggaagaa
    24601 gaagttgctt ttattcaaga aaaaatgtct gaaggtactc aaacaaacga agatacagtc
    24661 aaatgggatc cagaagaaac agaacttgtt tcaacgaaag aaaaacaaat caaatttgat
    24721 gtatttgaaa aagtagaact aaaagtagca gaagtcatca actgtcaaaa agtagaaggg
    24781 gcagataagt tgctgcaatt ccgactagat gctggagaca gccaagaccg tcaaatcttg
    24841 tcaggaatcg ctgaatttta tcctgatcca agcgaattga tcgggaaaaa agtcgtcatt
    24901 gtagcaaatc tgaaaccacg aaagatgcgt ggacaaatca gtcaaggaat gatcctttct
    24961 gcagaagcac cagatggctc tttacaagta atcgaagcac caaaatccat gccaaatggc
    25021 tcagaaatcg catagaaatt caaagggggt atgacactag ttgtgtcata cccccttttt
    25081 ccgaaatttg agaagcaatt ttcgtaaatt ccttcttatt acttgaagcc aatcatataa
    25141 tacagaaaca gagaactatc caaaataaat cctagaaaaa gaagtacgag cttcgctgat
    25201 ttttgttaat ttagaataat actaatagga atcgtaaaaa cagaaaaaaa gcataatgat
    25261 aaagggtcgg gcagatttta attgattcat ctggtactct tttttattgt ccttacattc
    25321 at
//
LOCUS       DACDIG010000039        24594 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.039, whole genome shotgun sequence.
ACCESSION   DACDIG010000039 DACDIG010000000
VERSION     DACDIG010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.039"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..374)
                     /locus_tag="IV247_001642"
     CDS             complement(<1..374)
                     /locus_tag="IV247_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777714.1"
                     /translation="MADIVQLKEDGVAKYLKTHVKAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVLEGYRTPISFEQLVGS"
     gene            complement(374..823)
                     /locus_tag="IV247_001643"
     CDS             complement(374..823)
                     /locus_tag="IV247_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777715.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            complement(827..1447)
                     /locus_tag="IV247_001644"
     CDS             complement(827..1447)
                     /locus_tag="IV247_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777716.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            complement(1447..2355)
                     /locus_tag="IV247_001645"
     CDS             complement(1447..2355)
                     /locus_tag="IV247_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HAP8777717.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETRIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            complement(2368..5127)
                     /locus_tag="IV247_001646"
     CDS             complement(2368..5127)
                     /locus_tag="IV247_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP8777718.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGEMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAMTSKGEIVADFI
                     KVGIINAEVLESSINDLGDVLKIVKGTLQIWNDNKKIMELTKKGMEFWNTKESIGTIG
                     TTDSAGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGDL
                     DVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWRIYDILCNNGFTKQ
                     SACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLFN
                     AAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGFKACTDPRQAAYAFERNYER
                     PAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSG
                     YLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINW
                     ARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAISSI
                     VIPPK"
     gene            complement(5124..5828)
                     /locus_tag="IV247_001647"
     CDS             complement(5124..5828)
                     /locus_tag="IV247_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP8777719.1"
                     /translation="MKPDEPNFIWKNRNAVIDMDCIIEGELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLDAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(5840..8149)
                     /locus_tag="IV247_001648"
     CDS             complement(5840..8149)
                     /locus_tag="IV247_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP8777720.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAVESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGESVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGGVKSF
                     IGVWQGLAGLFGISGGWFALAVIAIGALVTGLIWAYNNVKWFHDGMNSFFKGVSDVAV
                     EIFNFLGGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRRGIGGKVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANQQAIYDLGGVPI"
     gene            complement(8344..8808)
                     /locus_tag="IV247_001649"
     CDS             complement(8344..8808)
                     /locus_tag="IV247_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777721.1"
                     /translation="MARLSDYGINVEDLKNSATVTIQGVEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLYKKEGKISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKTQAKRKVNKKNRKR"
     gene            complement(8808..9434)
                     /locus_tag="IV247_001650"
     CDS             complement(8808..9434)
                     /locus_tag="IV247_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP8777722.1"
                     /translation="MGKQDTLYFEGLDDILIAMMATPDTVGTAPTYSEVVRLPIGTKI
                     GVKGNGTALEKWASSKMFRRVSRETKHEIALDHVGIPIEVMDEIKGLVARSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANFVSI
                     GLKYNNVIYASFDSNREEATMDLFEKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(9441..9806)
                     /locus_tag="IV247_001651"
     CDS             complement(9441..9806)
                     /locus_tag="IV247_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAP8777723.1"
                     /translation="MPGNKNGFSEMADYLGNLSRVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMSEQIKVVVDDDRVKVQFEETAFYWRFVENGATNRRAQHFASGTYEQ
                     NKEKIEEIMTKKILDLWEG"
     gene            complement(9796..10134)
                     /locus_tag="IV247_001652"
     CDS             complement(9796..10134)
                     /locus_tag="IV247_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777724.1"
                     /translation="MSISFDELKTALKSTKLPVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAR"
     gene            complement(10124..10450)
                     /locus_tag="IV247_001653"
     CDS             complement(10124..10450)
                     /locus_tag="IV247_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP8777725.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(10431..10709)
                     /locus_tag="IV247_001654"
     CDS             complement(10431..10709)
                     /locus_tag="IV247_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAP8777726.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETD"
     gene            complement(10711..12087)
                     /locus_tag="IV247_001655"
     CDS             complement(10711..12087)
                     /locus_tag="IV247_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP8777727.1"
                     /translation="MDTELLKKMKARREQRLTELREKVESGELREADLEAITKEIDAV
                     VDELNGIKSELSEADRSDEGSDADEGTGGSDGSTGSDENRSGEGDEGDEGEGGDNDDS
                     ENRSGMISQQQRDGLLGSIKNGLEARAKMTNKQKDQQLRKAFANFVVGNISEAEARAL
                     GIEAGNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKK
                     ERSTDIAETAIQFDEILLDPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEI
                     NYMFNGDDTGNENPGALAKKAVAFEKPLDLTAAGAGQKLYDALIEFKNTPVTEVMKKG
                     RFIINRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTP
                     VLYFGDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRY
                     EITKPVGG"
     gene            complement(12100..12675)
                     /locus_tag="IV247_001656"
     CDS             complement(12100..12675)
                     /locus_tag="IV247_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HAP8777728.1"
                     /translation="MEMKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSPTLLWD
                     DLSEVIAPGAFARTISNSDVRCLFNHDWSNVLGRTKSGTLRLSEDDHGLKFEVDLPDT
                     TVARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTISEVELYEVSIVPLPAYED
                     TEAALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(12641..13828)
                     /locus_tag="IV247_001657"
     CDS             complement(12641..13828)
                     /locus_tag="IV247_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP8777729.1"
                     /translation="MEKRGYIEDMFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALSSPVVIGPDGNEVKDHHLLKILKNPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNDSDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQNLKGGDGNEEEGN
                     SHD"
     gene            complement(13892..15619)
                     /locus_tag="IV247_001658"
     CDS             complement(13892..15619)
                     /locus_tag="IV247_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP8777730.1"
                     /translation="MIEPGVNYADLFAKEVRKKPGKYPKTVRLAVDRWYRWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFILEPWEKFIYSWMYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAATILN
                     NPILLEGTKIFKGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGNLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MDLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(15616..16068)
                     /locus_tag="IV247_001659"
     CDS             complement(15616..16068)
                     /locus_tag="IV247_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HAP8777731.1"
                     /translation="MTTKAQRKAIVDEKVSAEKARILKIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWSDKKMKALDLLGL
                     TNKAKTGRQITGGSTARKDEEITRPEEKPVDELAAHRNKWRKKAGTEK"
     gene            complement(16179..16559)
                     /locus_tag="IV247_001660"
     CDS             complement(16179..16559)
                     /locus_tag="IV247_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP8777732.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(16556..16957)
                     /locus_tag="IV247_001661"
     CDS             complement(16556..16957)
                     /locus_tag="IV247_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777733.1"
                     /translation="MAMIKIRTSITGTEYWDSEKKKTVVVPKGQEPKFDTSEQTDMTV
                     FAKAIVGDGVLFKSGEEIHLSGEELDSDGNTAADFDGDEATNDQSTEDTDELDNMTAK
                     ELRAYAKKHGIDIPGAVRAKGDIIHLIREAE"
     gene            complement(16938..17324)
                     /locus_tag="IV247_001662"
     CDS             complement(16938..17324)
                     /locus_tag="IV247_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777734.1"
                     /translation="MNDFHEAVLSIEVEPSLATAYKKAIEDDNSRHWIKNEIKDADGN
                     IVISDIKPSWNGNHCSVSIDNSLTIEKYSGAIVDRTERKIQLTIALLSRTLPNLQEQV
                     EWYERMGAKVISTNYKGENQNGNDQN"
     gene            17807..17986
                     /locus_tag="IV247_001663"
     CDS             17807..17986
                     /locus_tag="IV247_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HAP8777735.1"
                     /translation="MYFVIREATNGQYYFVIKSNNNEVVATSETYLTKYSAEQTINSI
                     KNGITKDSQVIDMTK"
     gene            complement(17979..18392)
                     /locus_tag="IV247_001664"
     CDS             complement(17979..18392)
                     /locus_tag="IV247_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777736.1"
                     /translation="MDKEIKATVKLDLTELKELLNKASDQVEQIKYQGMDRKRLIDII
                     EQQRLELIQKEAVCKEYKKHLEQVIEYHSVEKYRSVVQKNRATSPDPRQDVSISIKTP
                     VFNSVKSEKLESISTALVEKAAEMNSLANEVSRLI"
     gene            complement(18416..18652)
                     /locus_tag="IV247_001665"
     CDS             complement(18416..18652)
                     /locus_tag="IV247_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777737.1"
                     /translation="MYWRVYKMKIASEKIDQLTILVNQRIEDIIKEFEGEKIKNIKLT
                     LMDHKFELNSTKRKIVELIEEEIALQINEQQLTN"
     gene            complement(18941..19354)
                     /locus_tag="IV247_001666"
     CDS             complement(18941..19354)
                     /locus_tag="IV247_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP8777738.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            complement(19431..19727)
                     /locus_tag="IV247_001667"
     CDS             complement(19431..19727)
                     /locus_tag="IV247_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777739.1"
                     /translation="MKLKDGFYSSSHGIGGLMLDMPTKNPKTRKKTKVKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMECDKRLAKSKENLAVARLVDMEVIKSK"
     gene            complement(19751..20056)
                     /locus_tag="IV247_001668"
     CDS             complement(19751..20056)
                     /locus_tag="IV247_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777740.1"
                     /translation="MNVQVYLKRDLARFPRSWDEYSCPFEAEEESYLMSANMIEITRD
                     DADEFDKKRSWYSVSNPMYTAVIEDYHISDRFILVDLERPKKRIKQYRRCGFSTKKW"
     gene            complement(20053..20376)
                     /locus_tag="IV247_001669"
     CDS             complement(20053..20376)
                     /locus_tag="IV247_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777741.1"
                     /translation="MSDYLTAEQYEYARKTGDWESGKVLTHGQALNNNQKIVLEYLKS
                     KITESSVNPINAIVILGHLWEFRRFNTEEECLVLEAYWQLNSKQQVEVLQAFSAWVLE
                     EEEAE"
     gene            complement(20373..20651)
                     /locus_tag="IV247_001670"
     CDS             complement(20373..20651)
                     /locus_tag="IV247_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777742.1"
                     /translation="MKYRKKPVIIEAVQFNRSKAEKDVAKYYPMVTDMARLTTAKGTE
                     ERENRFFISTLEGNMTVKDGDYIIQGVEGEFYPCKPDIFEKTYEAVEK"
     gene            complement(20648..21232)
                     /locus_tag="IV247_001671"
     CDS             complement(20648..21232)
                     /locus_tag="IV247_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAP8777743.1"
                     /translation="MNKQELIDKLSSLVGSIEDFKGISTFHAGKYAGLEHALELIQQL
                     NESQKPVIPQLVAGWIEKSTDNLTKAEKIAYLIKSKDGDSYYFCDWFVRDGILTQEQG
                     EELLAWATRQSYETLLSLYNGYEVEKEPLYEVIIGDLYLIKKFNNRNDFCFDTSRSLC
                     TWEKSAYQLTEAEIKEIDERYWPFAVPVEEVAER"
     gene            complement(21229..21642)
                     /locus_tag="IV247_001672"
     CDS             complement(21229..21642)
                     /locus_tag="IV247_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002393044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777744.1"
                     /translation="MIPKFRAWVKPDILSNHLNGVIAEALPNFIYGNCLVLREDLEED
                     KQFEEIIDFLDIELMQSTGMKDKNGVDIFEGDIVKTLDADLKVTFSTIKFEEGAFCVD
                     YKDLGTEFDFLYFVDSPLEVIGNIYENSELLEEQR"
     gene            complement(21626..21958)
                     /locus_tag="IV247_001673"
     CDS             complement(21626..21958)
                     /locus_tag="IV247_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAP8777745.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDTEV"
     gene            complement(21958..22263)
                     /locus_tag="IV247_001674"
     CDS             complement(21958..22263)
                     /locus_tag="IV247_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777746.1"
                     /translation="MSVDKKATMKRIAELTKSESWQEDKEIVAEVQKLGKSMWAEKTK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEGK"
     gene            complement(22260..22421)
                     /locus_tag="IV247_001675"
     CDS             complement(22260..22421)
                     /locus_tag="IV247_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP8777747.1"
                     /translation="MTTEEVIQMRIRNIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            complement(22436..22795)
                     /locus_tag="IV247_001676"
     CDS             complement(22436..22795)
                     /locus_tag="IV247_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAP8777748.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQA
                     PLILAKKYRNTFKEERF"
     gene            complement(22792..23643)
                     /locus_tag="IV247_001677"
     CDS             complement(22792..23643)
                     /locus_tag="IV247_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8777749.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALEKAKSYTNEVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNTELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            complement(23660..24490)
                     /locus_tag="IV247_001678"
     CDS             complement(23660..24490)
                     /locus_tag="IV247_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP8777750.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
BASE COUNT         6689 a         5470 c         3789 g         8646 t
ORIGIN      
        1 gacccaacca gttgttcaaa actaattggt gtccgatagc cttctagaac agttaaaata
       61 accgaatcat tcccgccact actttttccg tttaaagcca caaaatatat agaaactgtt
      121 tttccttcac gataaagctt tgctgtccct gtattaccgt tagtgacaac cagatttacc
      181 acttcatatc tgttatcatc agtcgtcaaa acacgtttat tattgattgt tacagaacct
      241 gaaaaattct tatgtcctgc aatggcttgg tctccatctg tctgcaccaa tgcttcttta
      301 ccatcaatag cttttacatg cgtttttaag tatttagcaa ctccgtcttc ttttaattga
      361 acaatatcag ccattaaacc gtccccactt tctcaaatgt aatattggct aatccatcaa
      421 gtttcacttt atcggttgct gacattaaac ctgctgtcgt agttgtagca ttacctggat
      481 ttttctgtgc tccagctgca attccatcca acttagtttt atctgtggac gacatcaacc
      541 catttgctgt agttgtagct acagctgtag ttgtggcatt tattccagga tcacctttat
      601 ctccctttgg taaaacaaaa ttaaatctag ctgcagatga tgttcctaca ttcgtaacag
      661 aagcggttga accactagaa acggttccta tggtaattgt tgctgcttgg ccaggatcgc
      721 ctttatctcc cttcaccgtt gttggtttgc cttctatagc attccaatga gtttgtggat
      781 aaacctgtac attgctttgt tttattttta cgatatctgt cattctctat acctccccaa
      841 ttttttcaaa agtaaaattt ggaattcttt cgtttgtgta attttctgct tgatttacag
      901 cttcttggaa tttttgatct acatatgact gattgacacc accagtccca ctaccacctg
      961 tagaactaat tgttccatct tcatcaatag tgatgttttc tccagcagta taatttttca
     1021 gtgaatcgag tttttctttt aactccagcg aaaaattgac atcagattgt ctgactgcag
     1081 agactttccc tgtctcatca atcgtcaata attcaccaac ttttatgcca cccaaacggt
     1141 cgatagtggc aactggcaac acgtagtcac cttctccact gtttaaaatt ttttgataca
     1201 tttctgccgt gataatgccg tcatcttctt cacttgcata gggtaattct gtgagaacat
     1261 tctctaatcc caaatcttct ttagtaatga cgacggctcc aatttttcca ttaacagata
     1321 aaactgttgc ttctcctgct attatttctt ctaacccaag aactgcggaa gcatgtgtga
     1381 ttggaaagaa ttggcgttgt acacctgttt tttcatccgt ttccatcatt cgtttagttt
     1441 tagccattag atcaccccga ctttctcaag agtgaacaca ttttgctttg gatcatcaac
     1501 ggttgcgatg atcaaggctc cttcttcaat tggaaattct actgtttcaa ttttttcgac
     1561 ttcgtgatta tctgaaaaga gatcgtcttg aagaatatca gtcacttcaa tttcaccgta
     1621 ttcaatcgtg aaaagtgctg ctttcatttt ttgatacaaa taatttagat cggataggaa
     1681 acgttcagaa atcgattcat ggcgtacacc ttgaagatct acacgtgcat ccattaattc
     1741 ggctaacatt actccacctg gatcgataga cttcaaaata tccttgattg attcaaacca
     1801 tgacaaataa tctgattctt gtccttctct ccaatcttgg aaactattct cctgttcttc
     1861 tctccagcga tcaaattctt ctttcctttc attcatccag tccgtaaaat cgcctttatt
     1921 ttcattaata aaatctgtca tatcagcaat taaatcttca attgattgcc aataagaacc
     1981 catttctcct tctgtttttg aaacagcatt gatgacaaaa taggagaagt cttgagttgt
     2041 tccaatcaag tcttctcctt taaatataat gaaattggct gtttgtcgat gcaaacactg
     2101 catggaatat ttatcaaaaa tatatttgat tttccctttt ttagcatcca caattcttgt
     2161 ttctaattgg actggatatt ttccgccaac aactgattca aaatatacct tacattctga
     2221 taaatcatat ggaagaccat tttcgacaat tgtagcttcc ataacttcag tgtttttatt
     2281 gccttgtcga acttgaatca tccccacgta attatagggt tctgttgtac ttaatatgac
     2341 attccacttt gccattaaat cacctcccta ttttggtggt atgacaatgg aagaaattgc
     2401 acttgcacta taatactggc gatctaactt tccacaaatc atacctaact ctgtgttttg
     2461 ttcataagtt tgcatacgac cattagctaa gcctcggata acgccagtat gtccataagt
     2521 tccgtctgca aaccatgaac ccacttgtcc acctcttgcc caattaataa ttgcaccaac
     2581 tactaattga tcatatctag ggttttgaat cactttccaa ccaacagcag accaatcata
     2641 ggcaatacca atatcagatg ctgcagaagt atttcctatc acatgagtta aaccatattt
     2701 tgttccagca cccatgccac aaccacccag atatcctgaa tattctgcag acaagccata
     2761 acactgacca ttgccaattc gttgtccaat caaagactct aaatgtttta gtcctgcttc
     2821 tccagtagct cctcctggtt ttaaatcttt aaatttgtta taccagttaa ctgcataatc
     2881 ttgacgttca ggatgtgtcg ctgccggacg ttcatagttt cgttcaaaag catatgctgc
     2941 ttgtcttgga tcagtacagg ccttaaatcc atcaaccgtc gtaggttgta ctacgcccat
     3001 ccattgtcca tttgtaaatg tccaaataag caatcgaact tgtgcatcta agctcgttat
     3061 tggttcttta atacctgcag cattaaataa gttttgaaca taaacttttc catcccatgt
     3121 tgctggacca acaagaggat atgaagaacc atcccactga actaatccgt atgctggtcc
     3181 acctatttga acagtatcag gatcaaaagt tcctcctgtt tcttgttgaa tgttccccaa
     3241 tattccacat gcagattgtt tcgtaaatcc gttattacac aaaatatcgt aaattctcca
     3301 agctctcttt tctgcatctg tttttaattc actaggatac ccaccagtag attctccacc
     3361 tcccgatgga ccaccacctt gtccaggaat tatttctttt cctttaactg taagttttcc
     3421 ttgtacatct aagtctccaa aataaattgc tttaccattt cctaataaaa ctaatccttt
     3481 tcctacttta ggagaaatca aaatatattt gccgtctcca tttgtacgaa taactaaaga
     3541 attatcttca ataggggttg gagtagaagc cccaggaaaa ggattgcctg cagaatcagt
     3601 agtaccaatt gtgccaatgg attctttggt attccagaac tccatccctt ttttggttaa
     3661 ttccatgatt tttttattat cattccaaat ttgtaatgta cctttgacaa tttttagcac
     3721 atcgcccaga tcattaattg agctttcaag gacttctgca ttaataatcc caactttaat
     3781 gaaatcagct acgatttcac ctttcgacgt cattgcgata ccaaacgggc catttactcc
     3841 gttgtctgaa taacctaagc catttaaatt ccaacgccac acacgtttcg cattcgctac
     3901 atttggagta tccatgataa gaatctctga tggagctttt tctggacgaa aaacgacatg
     3961 cccaccagaa ttaccagtga tccatgccgt cgcattcaac acattttgaa ctaacgtttc
     4021 tgttcgattg tcgattttct ttttcaattc ttgtgcttga gtatttactg ccgaagtgta
     4081 cagtgataaa tcattaccta aaacaatatc cttatatttc ccaagcgttg ggaaataagt
     4141 gtattcgacc atgcgctctt ttatctcgat atctagctct ttcgccctaa catgagcgac
     4201 atcaccaaag tgtaaagacg ctagctcttg atacatctca ccgtattcga gtgtgtgttc
     4261 caacgccacc atactgactg tgtgtgtagc ttttggctca tggatacgat cattgtcaaa
     4321 taaggttttg ccccatttga tcaattcatc aactgtttta caatctccgt tttcacgttt
     4381 ggcaatacgc cgattctcgt tggttactcc atcaatttct aaataaccat attcgatagg
     4441 ttctttgtct tcatcataat cgttatcagg aacaccgcca accaaataca aactattgac
     4501 aattgattct tcatcaattt cttcttcgat tgcttctaaa ttgatgccaa aatcaatccg
     4561 aaaaccatta tctgatccaa tctgttttac cagtttcaaa tcatagttat ccatatcaag
     4621 ttccgcgcta gtcacgccag tcaaattttg attaccgttg tttgaaccaa tgattgcttc
     4681 aactggtgcg acttgtcttg ctgtaaattg gtgcgttgtc cctacattcg aaagatagtt
     4741 aaatttttga tcaaatgcca aactgttttt aagattggtc atgatctgag acccattgcc
     4801 gttatccgta aacgacttga caataaagtt cttattggcc ataaaaccaa tatgccgagc
     4861 tgttacagaa acggaggtca aattcttttt gacattataa atttcaaaat attgccacga
     4921 accatccggc accttcgctt ttataaaatt tccttttttt aaataagagc gatactgtcc
     4981 gcttcttgaa tagttaccat aaaaacgata ttggccattc agcacacgat tgatttctgg
     5041 caaatcttcc caatcaacta acgacgctcc gttaacactc aaatcatctg gcattttttt
     5101 gtaagcataa ataaattctt gcgtcacaga tatacactcc tattccaaaa ccgcacttct
     5161 ttaaattttc cggatatttt tacctgattc cattcaggct gcaatactgg ccaatcacca
     5221 cgcgtaaata aattcaaacc ttcttgtatt gctttcccaa gctgagtatc aaccacgatt
     5281 gttgcagcta aagtattaag tattgttagg cttttgtcac cgacagcgat cgtaatatct
     5341 cctccgttcg attctatttc cagataagga tgtgcaattt cgtcaccatg atcaaaaata
     5401 tccataacac tagaatgaaa aattttaggt gcttcaccga tttttctttt gagtggctga
     5461 caacgaaaag taacatcgaa tgtataaaag aagccccact cgttttcgaa tgggacttct
     5521 ttatccatgc tgcagatagc atctagatac ttgtctggat cattatgagt gattaattgg
     5581 cttttaccag taagccatcg tttgacttcc ctcagtttcg aatgtgggat agtgatccct
     5641 tctatttcca aatcaaaagg ttcataatca tcaaacgtct ccgtcaattc tccgcttcgg
     5701 ccctgaatcg tgtaagtttc gtatcgcttg ttcggcataa tatctggaag ctctccctca
     5761 ataatgcaat ccatatcaat tactgcattc cgatttttcc aaataaaatt gggttcatct
     5821 ggtttcataa attgtcgaat caaataggaa caccccccaa atcataaata gcctgttggt
     5881 ttgctttagc aaatttacga ttcatacgat ctaactcaga tggattgttt gcatcgactt
     5941 tgccaatgtg tatatgctgc tcaatactac caccagacac ttttccgcca atacctctgc
     6001 gtttttcttc gtcagataac ggagtaactg tagttttacc attttttgca gtcagtaact
     6061 ctggaccagc ttcaccaaca atcgcttgtc cattaatcag atgaccccct tgcgctaaat
     6121 aaggcagttt agaaatagaa aatgattttc cacctacacc aggaacccat tttggtatct
     6181 ttatattatt caatccacca ataaatccat tgatcaatcc aatcatggca ttaataggag
     6241 ctttccctac agcaacaata ccatcgaaaa tacctccaaa aatatcaact agaccttgcc
     6301 atgcttttga ccaatttcct gtaaatactc cagtaataaa atctagaaaa ccactgaaaa
     6361 ttcgttttcc agcattataa aaattattga aatttgctat aacaccatcg aaaaatccac
     6421 caaaataacc acctaaaaaa ttgaatattt caacagctac atctgaaaca cctttgaaga
     6481 aagaattcat tccatcatga aaccatttaa cgttattata tgcccaaatt agtccagtaa
     6541 ctaatgcgcc aatagctata actgctaatg caaaccagcc tccagatatt ccaaataaac
     6601 cagcaagtcc ttgccaaact cctataaagc ttttaacccc cccaacgatt tttgtaacag
     6661 atcccataag tgaaccaaga acgattagta ctggtccaac tgctgccgct ataccagcaa
     6721 ttgttatgat ccaattcttt gtatcttgat ctagagaccc ccaccattta gagaattcct
     6781 gtaataagga agttgctttt tcaaaaaaag gcaaaaggct tacttggact gattctccta
     6841 cgtcagccat tgctaatttt gcattgttca tagcttgatc ggcttgatca attgggtcga
     6901 gagtagcatc gaatgtgtct cctacagctc ccccactttc tacagcagtt tttgctaaat
     6961 cttccaggtt caaagtacct ctacgaattg catcggccat tctaggaccg cctttagtcc
     7021 cgaatacttc tgctgctgca ttgatggctt ctgtttcaga acctgcattt tttaccttgt
     7081 cttgtagttc ttctagtcct tgacttaaag attttccgtc tttcgcataa gccactgttg
     7141 ctttagataa actacttaaa gctgcgcttg aatcaacacc agattgctca aattgtccaa
     7201 gtaaagtgac cccttcacca aagctcaacc ctaattgttt aatttgtggt gcgccatcaa
     7261 ttgctttttg catcaagtcg tctacagatt gaccagtatt ctgcgatgtt ttcgttgtta
     7321 catcaaggac agaattcaaa tcatcatatt ctagtccata agcatcaatg gcttgtcgag
     7381 cagatattgc tgattgcgaa acatctgtat cattgatttc agcgtactgt aggagataat
     7441 tggttgaatc ttccagtttt ttatccatga atccaaattg tgtatttacc tcaccaatag
     7501 cttccccaac cgtttgtaaa ggtaaatgag tatttgaacc aacgtttttg aaagactgtg
     7561 aaagtctatc agcttgatca cctgttgctc cggtttttgt aatgatggta tcaagtgcct
     7621 cgtcaacttc accaaatgct gcaagtcctg ctgctcctgc cgctacaata ggcgcggtta
     7681 ctccaacaga cattttttca ccaacacctt ttactttctc acctgtttct tcgattttct
     7741 ggagtttttt tgctgtatca acagaaacgt caccttgttc tttaagtgca tcgttcgtct
     7801 gttctaatgc tgtacgaagt ttattttctc cggtttcaga attgagtaat tgtttataaa
     7861 gtttttctga ttcagcagaa tattcgcctg tctctttgac agatttttca tattcttctc
     7921 tcaacatttg agatcgttgt tcagctaacc ccagttgttt ttctaacttt tgtttagccg
     7981 ctcgcaattt ttctgtttga gttgcatcct tgtccatcgc agacacttgg tttttgtact
     8041 cggcagcggc taagttcatt tctttgttga tttctttgat tgttttcgaa taactgactt
     8101 cgccattcgt tttaaaatta agaacgacat cagattcttt accagccatt tatcttagcg
     8161 ctcctttcta ccaccaaggc gatttatcca tagttacaga ttgaggtggt tcaaaatcgg
     8221 tgttttgttg taaccactgt aaataagatt tgagccacaa gttaggtgta gacttcaaaa
     8281 agaaactctc actccaattc aatagagtga gagcaacgta aatataaaaa ctccaaggag
     8341 ttcctatctc ttccgattct ttttgtttac ctttcttttt gcttgcgttt tttgaaagtc
     8401 ttgtggcttc ttggattttt ttaggtcttc cacctgaaat gtctggctag caaaaatttc
     8461 catacaggta ctataagcag acaacacctc tccactcatt cctaagaatt taaaaatagt
     8521 ttctggatct tcctctaaac caccagtgcg caacattgca taaatcaagg cacgcatgat
     8581 ttttaagtca ctaggcgata agttagcaga agaaattttt ccttcttttt tgtatagcat
     8641 ggcattcata tcagattcga attgcgaata atctccacca tatacatctg ctataaattc
     8701 cattgtttgc atagtaaatg agatagggaa ttctacacct tgaatagtga cagtagcaga
     8761 attttttaag tcttcaacgt taattccata atcagataac cgagccatta gcctgcacct
     8821 ccaactttag ccaatgtttt ccactgttct tcatcgtata caggttgagc aatgaatttt
     8881 tcaaatagat ccattgtcgc ttcttcgcga ttcgaatcaa aacttgcata tattacatta
     8941 ttgtatttca gtccaataga aacaaaattt gcagttacat cgtcgatttt tgtctcttct
     9001 tctgcagtgg catactcttc atcaattaca ttagatagtt gtgtgttagg ataccatacc
     9061 gcttttttac cgccattctc aatatttccg atgaatccaa aggcaaaata cggaaattct
     9121 cgtgcggtat ttttgctaaa cgttactccg cttctagcaa ctaatccttt tatttcatcc
     9181 attacttcaa tcggaatacc cacatgatcc aacgcaattt catgttttgt ttctcgcgat
     9241 acacgtcgga acattttact tgatgcccat ttctctaacg ctgtaccatt acctttgacg
     9301 ccgatttttg taccgatagg caaacgaaca acttcactat aagttggcgc agtcccaacg
     9361 gtatcaggcg ttgccatcat cgcaattaaa atgtcgtcga ggccttcaaa atataaagta
     9421 tcttgttttc ccaattaaaa tcacccttcc cataaatcaa gtattttctt tgtcataatc
     9481 tcttctattt tttctttgtt ttgttcatat gtcccacttg caaagtgttg ggctcttcgg
     9541 ttagttgctc cattttcaac aaatcgccaa taaaaagcag tttcttcaaa ctggaccttc
     9601 actcgatcat catcgaccac aacttttatt tgttcgctca tatgtttttt cttaagcagg
     9661 gacttaggaa tattgggaag caactgcttt agataaaagt tagctgcttc ttctaaggat
     9721 tccaaggata attttttggg atcaactctc gaaagattac ccaaataatc agccatctcc
     9781 gaaaatccat tcttattacc tggcattttc cacacacctc acatatgtgt aatagttggt
     9841 cacggtatcg tcattctcat caccttgtat acctgtaaag tctgaatatg gaatgccagc
     9901 attttgcagc gcttgttcaa taacaactaa atcctgttct gttccaagtg taaaaaaaga
     9961 gacttggtaa taaggtaatt ttttatagac tttaccggat gccatttttt tgctgttact
    10021 cacatttgag tacacaatgt atggatacat cgtccctaat ctggctttgt ctctgaacac
    10081 tggtaacttt gttgatttca gcgctgtttt caattcatca aagctaatcg acataagcta
    10141 aactcaactc catttctctt gcatcgggat tcgtataaat gcgagtaatg ttatacgtta
    10201 cagaatcaat tttgagcgca cttaatttct ctgtgatgga tttatcccat ctgactttaa
    10261 ttcgtctgac aacgtctgtc ttggcttgct gtgataaata tttttcttga gaagtcacac
    10321 cgagttcttc atagaatatt agacgcttaa attcgtaaat tgtagttgga cgatcgtttc
    10381 cgtctgttcc tgttttgatg tctagcaatt cggctttcca tctgagatta ttagtctgtc
    10441 tcttcggcat tttgaatcgc tccttgcacg ataaatggcg tcatggcatt catagctttg
    10501 tcgagttcat cctctgaaac tctatattca taggcaatgc cggcaaccat caaaataaga
    10561 tattcttgtt ggccaccagt tgctgttttg acataatctt ttgccatatt taaataaaaa
    10621 gagagcaaag aatcatccat gccctcttca aaatgaatat gtgatttgaa tttttcctct
    10681 aaagacaatt ctttagtttg ctcttccatc ttaaccacca actggttttg taatttcgta
    10741 gcgatatact gccggttcaa atggagaata aaccaattga ccatctagca agttgtaaat
    10801 ttggaatcca atttgatttt taccagagaa tttttcaaca agtttttgaa tttccaaggc
    10861 accaataact tcttgaattt taaatgcaga aaaatcgcca aaatataaaa ctggtgtgtc
    10921 tggttcaccc tttttatctg ctgcatctgt ccaatccaca ggatagccaa ctaattggta
    10981 accaattcca ccttctgctt gtgtgaatgg ccgcaacaaa ggaaatccat catctgtttt
    11041 cattttttca atggcagtca aagcagctcg attaataata aagcgtccct ttttcatcac
    11101 ttctgtcact ggtgtatttt taaattcgat taatgcatca tataattttt gcccagcacc
    11161 tgcagcagtt agatctaaag gtttttcaaa tgctacagcc tttttggcta atgcaccagg
    11221 attttcattt ccagtgtcat caccattgaa catataattg atttctttac gcacataagc
    11281 ttttttcaat tcttccacaa caatatcttc aactggaaca ccagacattt ttagtaattt
    11341 tttagttact gttgccaaag catcgaattc ggcaggatca agcaaaattt catcaaactg
    11401 aatagctgtt tcagcaatat cagttgaacg ctctttcttg tttacattcg catctgcttt
    11461 cttcacaaga attggatatt tgacatctcc tgatgttcgc accactgttc cgtatttacg
    11521 aagtaaattt tcttcttgag cataagtaat aacttcagat gcaattactt ctgggacagt
    11581 aactgaaccg ttgccagctt caatccctaa agctcgagct tctgcttcag aaatatttcc
    11641 aactacaaaa ttagcaaatg cttttcgtag ttgttgatct ttttgtttat tggtcatttt
    11701 tgcacgtgcc tccaatccgt tcttaattga tccaagtaat ccgtctcgtt gctgttgact
    11761 aatcattcca gaacgatttt ctgaatcatc gttatcccca ccttcccctt catcaccttc
    11821 atcaccttca cctgaacgat tttcatcgct tccagtcgag ccgtctgatc cacctgttcc
    11881 ttcgtcagca tctgaacctt catctgatct atcggcttca cttagttcac ttttgattcc
    11941 gttcaattca tcaacaacgg catcaatttc ttttgtaata gcttctaaat cagcttcgcg
    12001 taattctcct gattcaactt tttcacgtaa ttcagtcaat cgttgttcac gacgtgcctt
    12061 catttttttc aataattctg tatccatgta tttttcctcc tacgcttcta gcgcttgatt
    12121 aatttttttg attaattttt ttctttcttc gacgtttttt tctaattcat cacgacttct
    12181 taatgctgcc tctgtatctt cataagccgg caaaggaaca atagaaacct catataattc
    12241 cacttcgcta attgttcgaa gcataggttc tgaattgtag tcccatgttt cttcagttgg
    12301 tacaaatcca aaactacatt gattaatgtc tccgcgttcc atagatttaa ccaagtctct
    12361 tgctaccgtt gtgtctggca aatcgacttc gaatttcaaa ccatgatcgt cttctgacaa
    12421 tcgaagggtt ccgcttttgg ttcgccctag tacattagac caatcgtggt taaataagca
    12481 acgtacatcg gagttactaa tcgttctagc aaaagcgcct ggtgcaatga cttcactcaa
    12541 atcgtcccat aatagtgttg ggctattgaa tacagcagca tatccactaa tggtcctcgt
    12601 atgagtttct tcatcagaac gcgttgaaag gttggtgatg tcaatcgtgc gaatttcctt
    12661 cttcttcatt tccatcacct cctttcaagt tttgatcatt cgttggtaag gaatcatctg
    12721 ttgcattttt ctggccaatc ctagacaagt catttgaaat atagatagct tgtgtttctg
    12781 gagtgttctg tttaggaaaa ccaagcattt ctgccacatt atctggactt gtaatcccag
    12841 tacgaacaat gttgtagcca atatttgttt ttgttgagta aggaacaaaa tccaaaatgt
    12901 taattttcca ttccactcga tagccagaat taggcataaa aaaaagagcg gtgtaatgtt
    12961 cgctcttgtt cttcaatatt ggtttgattg ctttgttgtg cagatacatc atcgcttttt
    13021 cgatgtctgt tttcatcaac gattgatacg tatcaacatt gattcctaaa aatttaccta
    13081 agtctttttt ataaacacct aaatagttca aaatagccgc gtcatcaaca ggacttttta
    13141 acgtctcgat ggaatatcct tttcccagag gaatcatctt aacagaatga tcactgtcat
    13201 tttgcgttcc ttccagttga tccaatatag ctttgacaat tttcgtttgg gcgctattat
    13261 ttggattgat gtgggcgtcc agtttaagca tgaacgcgag taaaccacct ttagtatatt
    13321 tgtccgtcaa aactttttca gcgctcagaa cgccttccag agtgtttctt gcaaggtcaa
    13381 ttattccagc accttttaat gaatcagttc cgatgttctt aatatgtcga atcatttgac
    13441 caggtatttt ttgtccattc atttcaaatt cttcttgaag tcgttcattg attttagtgg
    13501 ttacaccgta tgccaaatga agctggtccc gatctgttaa tgggaatgtc tcaccattga
    13561 tcagtagggt atttgtttcc aatttggtaa attcgaatcc ggtcaaataa tcattgggat
    13621 tcttcaaaat ttttagcaag tggtggtcct tcacttcatt accgtctgga cctatgacaa
    13681 caggtgagga caacgctacc tggtttgaga tatcctggac caattcataa acatcagaag
    13741 attccatgat agaggaatct gttacatatc tttgaccgta acgcgtatag tggccaaaca
    13801 tatcctcgat gtacccacgc ttttccataa aggaatagac tgcattcgat aaccgatcac
    13861 gtaatttcaa tatttctcac cgcctttcta tttatctata gatggaacta aggtaatcat
    13921 ctaattcatc cgaatcaata tctgtcatct gattcatcgt ttccttatga ccacacaaaa
    13981 acgccacgaa accatcaatc tttttctttg attgacgttt acttggcgct ttttgtccgt
    14041 tgatgttagt gattgctaca acgttcaagg tgcaataaag gaacaatgga ttatcaaatt
    14101 gaattcgttt ctcataaaac aaccgttcga catcatcaaa aggagcgttc aacactttag
    14161 gatattgggc aacctcaacg cattccaatc ctaagttctc caatttctca acaagtttgt
    14221 cgctcatcgc tggatcataa ttcacttgtt gtatatcgta taaatccatg cagtcttcga
    14281 tgtactgcaa gatttgatct tgatcaatca ttttaccatc gcaaaattca acaaaacctt
    14341 gttcagctaa atcgctgtaa ggcacgttat cttctttttc tcgaaactct aaatcttcat
    14401 tgggaataaa ataaagctgc ttcactttaa ggaccgcttt tccatcttca tcccacgttg
    14461 ggaagtttaa agacacacag gtcaaatctc gtgtgcgtga taaatccaaa ccaatgtaac
    14521 aaggttcacc acttaaattt ccaagctctt gggtagtaac caaacacggc tctacttgat
    14581 cctgttcaaa gaaattatcc gcaccgttta caaatacatc caagtgcttc gttagaaact
    14641 cggcttttga atgtgctgaa cgttgtgccg ttttaaacgc tgactccaag gcggaaagat
    14701 caactgatat tccccagtta ggattgcaca tttcccaaac ttttctatcc gtccaatcat
    14761 agtttttatt tggttcatag attagaacaa agttcgaatc attatcatca cgtttcaaca
    14821 cttcttttgc ttcacgatat acgcgcattc caacagacga tgaacctttc ccagcagttg
    14881 aaatattaaa catcaatggt tgtggcaaag agatttgtgc agacttaaaa ttgtcatact
    14941 gttccatttt ctcttgctta tgcagctcat cattcaatac aaaatatgga ttagaaccct
    15001 caatgttatc gatattcttc gtttgaacaa taaatttatt cgaataagcc atatcttcat
    15061 gaatataatc ataagtaata ctagaaacag ttccctttgg tcctttgaat attttagttc
    15121 cttcaagtag gattggattg tttaatattg tagctgcaaa aggtttggca gcatattgag
    15181 cttgagcaaa gtcagaagcg catgcataac aatcgactga caaagcacct tctccataca
    15241 ttgcatagcc taatgaaccg acagcaatca atgttttacc gttcttttta gggatttgga
    15301 catacgcttc acgagtaaca cggacaattt gccctttttc attttcttta acccatccat
    15361 acatccacga gtaaataaat ttttcccaag gttctaaaat gaatggcttt ccaaccatct
    15421 caccttttgt gtgaacaata aacgactcaa cccagtccat catttcattc gcacgatcta
    15481 catcaaacca aatatcttta cgttttttcc accgatacca acgatccact gccaaacgaa
    15541 cagttttagg atatttccca ggtttctttc ttacttcttt tgcaaataaa tcggcataat
    15601 ttacaccagg ttcgatcatt tttcagtacc tgccttctta cgccatttgt ttctgtgcgc
    15661 tgctagttca tctactggct tttcttctgg acgtgtaatt tcttcatctt ttcttgcagt
    15721 cgatccgcca gttatttgtc taccagtttt agccttattc gttagcccca acaaatctag
    15781 agctttcatt ttcttatctg accaagtttc tacttgctgc gccaatggat gcttcgattg
    15841 attagtggca cctgatttat tcgtgaattt ttgcgtctcc ggaaagcctt tttccttcca
    15901 caaaagatat ttgtgttggt aaatttcaaa aatatccaaa tatgattcga ttaatggatc
    15961 aagagtgatg gtgtacaaat cagacaaatt cattattttt aaaatacgag ctttttcagc
    16021 acttactttt tcatcaacaa tcgctttacg ttgcgcttta gtcgtcatac ttgtatacac
    16081 ccccctttgt tttttgaaaa atttgaccta acgatgcgcg tgactccccg ctaccctatc
    16141 tccccacgcg aaaaaatttt gaaaatggat agggggggtt aattaaaata cgaaggaaaa
    16201 acttttttgt cttctgtttc gttttcaaca attggatgac attttgaaca taaaagcatg
    16261 agattgtttg gatcaagctt aagcagttca ttatctttga ttggtacaat gtgatggacg
    16321 tgtgccctct ttccaaagat gaactgacca catcgctgac agtggccacc ttctctttcg
    16381 taaataaatt ggcgcatatc tttccatgct tgcgttcgat agaatggttt gttctcatga
    16441 tgataaacag actttgcttg ctgcttcttc ttgcgtgatc tgcttgatct cttgtgttca
    16501 gtacagtaga taccctttgc tatcttgttc gtgcatccgt caaactgaca gtacttcatt
    16561 ctgcttcacg aataagatgg atgatgtctc cttttgcacg gacagcacca ggaatatcaa
    16621 tgccatgttt cttagcatat gcacgcaatt cttttgcagt catattgtcc agttcatccg
    16681 tatcttcagt ggactgatca ttagtagctt cgtctccatc aaaatcagca gcggtgtttc
    16741 catcgctatc taattcttct cctgataaat gaatttcttc accactcttg aacaagacac
    16801 catcaccaac gattgccttg gcaaatacag tcatatcagt ctgttcactt gtatcaaatt
    16861 taggttcttg acctttcgga actacaacag tctttttctt ttctgaatcc caatactccg
    16921 ttcctgtgat ggatgttcta attttgatca ttgccatttt gattctctcc tttgtaattt
    16981 gtactgatta cttttgcacc cattcgctca taccattcaa cctgctcttg taagtttggc
    17041 agtgttctag aaagcaatgc tatggtcagc tgaattttcc gttctgttcg atcaactatt
    17101 gctccagaat atttttcaat agtcaggcta ttatcaatac tgacactaca gtggttgcca
    17161 ttccagcttg gcttgatatc gctaatcaca atatttccat cggcatcttt tatttcattt
    17221 ttaatccaat gcctactgtt gtcatcttca atcgcttttt tatatgcagt ggccagactt
    17281 ggttctacct cgatgctaag taccgcttca tgaaaatcat tcatagataa aactcctttc
    17341 aaaataaaaa gaccactcaa tgagtgatct aatatgtaat acaggaccca ctatccttcg
    17401 agggaatgtg gattacagaa acatcagcgc gctatagttt ccaacctgta tattcggttt
    17461 tactgacagg cagttatgga tcaccgtaaa ccaaagtcac tggcaatgaa tcgaacattg
    17521 caacaccatt ttccgatatt ttacagactc tccatcataa aaactctcac ctctagaatt
    17581 gtctatcttt aggggattta tttcaagatg cctcttgtcc tttaccctcg acggtctctt
    17641 gcctagctgc ataaactaag ctcaggtgct cattaccact gatttaagtt attagcgtta
    17701 cctttccgcc acagtgacaa atttatattg tgaaaataaa tactaagcgt ataattttaa
    17761 ttatcagcga gtggtccgct gaaataaatt ataggtggtg aaaaatttgt actttgttat
    17821 aagagaagca actaatggcc aatattactt cgttattaag tccaataaca atgaagtagt
    17881 agcaactagc gaaacatact tgactaaata ttccgctgaa caaaccatta attctataaa
    17941 aaacggaatc accaaagact ctcaagttat cgatatgact aaataagacg actaacttca
    18001 tttgctaggg agttcatttc agctgctttt tctaccaaag cagttgagat actttctagc
    18061 ttttctgatt taacagaatt aaatactggc gtcttgatac taatcgatac atcttgacga
    18121 ggatcaggac tcgttgctct atttttttgc accactgatc tgtacttttc tactgaatga
    18181 tactcaatta cttgttctaa atgttttttg tactctttac atacagcttc tttttggatc
    18241 aattctaaac gttgttgctc aataatatca atcaaccgtt ttcgatccat accttgatac
    18301 tttatttgtt cgacttggtc actagccttg ttgagcagtt cttttagttc agtcaaatct
    18361 aatttgacag ttgctttgat ttctttatcc attcgttttc cctccaatac ataaattaat
    18421 tagttaattg ttgctcattg atctggagag ctatttcttc ttctataagc tcaacgatct
    18481 tcctttttgt cgaatttaat tcgaacttat gatccattaa agtaagcttt atgttcttaa
    18541 tcttttcacc ttcgaattct ttgataatat cttcgattct ttggtttact aaaatagtta
    18601 gttgatcaat tttttcggaa gcaatcttca ttttatatac cctccaatac ataaattaat
    18661 agacagcagc agttgaaaga cgacaagaac atgtttagtt ttgtgagtgc tgctgtctaa
    18721 tcaaaataaa cagcaaccga tgaagaattt aggaggagtt gaattcacat ccttttcttc
    18781 ataattagtt gctgtctatc taagaagaag tttaaaacga tgagggagat tgcctccctt
    18841 cgtttatttt gtcgatcctg tttcctaatc tttcgacact accataataa cactggtaaa
    18901 tgtctaaaaa ccgccatcat tccgccaaaa aaccgccaaa ttatttataa gcaattattt
    18961 ttccgtgttt atatgcttct gcaaactcta ttagagcttc cgacttcatc cgttgtatac
    19021 ttctttctga ataacccact tcacggctaa ttctgtagtt tgagaagcta tctggcacac
    19081 agaagctgta gtagagtatc tgacgactaa tcagactaag agccatcaaa gccgctagaa
    19141 tcgcatctct ctccgtttct atatccatca tctgaataat cgcgtcttct gccttattgc
    19201 catgcttcgg tgccttcggc atatccgtta taatcggcga cttaatatct atcaaagagc
    19261 gacctgccat ccgctccaaa cgccgaaagt tcttcagcac atctctcgca ttacatcttg
    19321 tctgtttgaa atctacctct cgtaacaatt gcatcaagtc aaaccgctcc tttatgtgat
    19381 ataataaatg tgttggattt attgaatcag tcggagcgat ccggcttttt ttatttgctc
    19441 ttgattactt ccatgtcaac taatctcgct acagctaaat tctctttgct tttcgctaac
    19501 cgcttgtcac attccatcgt gttttcaatg cgaatgattg ctgagtttga cagtatcttt
    19561 ttaacgtatc ctcggaaggg ataaacgaac tcctctgctt cacagcgaac catgtcacca
    19621 acttttactt ttgttttctt acgtgtttta gggttctttg tcggcatgtc tagcatcaaa
    19681 cctccgatac cgtgactgct agagtagaat ccgtctttta gtttcattac tctacctcct
    19741 gttttaattt tcaccatttc tttgttgaaa acccgcaacg tctatactgt ttaattcgtt
    19801 tcttaggtct ttctagatca accagaataa atctgtcgga tatatgataa tcttctatta
    19861 cagccgtata cattggattc gaaaccgagt accatgatct ttttttatca aactcatccg
    19921 catcatctct tgtaatttct atcatgttag cagacattag atagctctct tcttctgcct
    19981 cgaatgggca ggaatattca tcccagcttc gaggaaacct ggccaaatct ctttttaaat
    20041 aaacttgaac attcattccg cttcctcttc ttccaacacc catgcgctga acgcttgaag
    20101 gacttctact tgttgcttac tattaagttg ccagtacgcc tcaagaacta gacactcttc
    20161 ctctgtatta aaccttctaa attcccacag atgtccaagt ataacaattg cattaatggg
    20221 atttaccgaa gactcggtaa tttttgattt aaggtactcc aacacgattt tctggttgtt
    20281 gttgagtgct tgcccatgag tgagaacttt acccgattcc caatctccag tttttctagc
    20341 atattcatat tgttctgctg ttaagtaatc gctcattttt ccaccgcctc atatgttttt
    20401 tcaaaaatat ctggcttaca tggataaaat tcaccttcca caccttgaat aatatagtct
    20461 ccatctttaa cagtcatatt cccttctaaa gtagaaatga aaaatctgtt ttcacgttct
    20521 tcggttcctt tcgctgtagt taatctagcc atatctgtaa ccatcggata gtactttgca
    20581 acatcctttt ccgcttttga tcgattaaat tgcaccgctt caataattac tggtttcttt
    20641 ctatatttca tctttctgcc acctcttcca ctggcacagc aaatggccag tatctttcat
    20701 caatttcttt gatttccgct tctgttagct gataagcaga tttttcccaa gtacacagcg
    20761 agcgactagt atcaaaacag aaatcatttc tgttattaaa tttcttgata agatataagt
    20821 caccaataat aacttcatac aacggttctt tctcaacctc gtagccgttg tataggctca
    20881 atagtgtttc atatgattgt ctcgttgccc aagcgagtaa ttcttctcct tgttcttgcg
    20941 tcaatatgcc atctcgcaca aaccaatcac agaaataata tgaatcacca tctttggatt
    21001 tgattaaata cgctattttt tcagcttttg ttaaattgtc ggtacttttt tcgatccaac
    21061 ctgccacgag ttgtggaata actggtttct gcgattcatt tagttgttgg attagttcta
    21121 atgcgtgttc taaacccgca tattttccag catgaaaagt acttatacct ttaaagtcct
    21181 ctatgctacc tacaaggctt gatagtttat caatcaattc ctgtttattc atcgctgttc
    21241 ctccaataac tcgctattct cgtatatatt cccgatgact tccaaaggtg aatctacaaa
    21301 gtacaaaaaa tcaaattccg tgccgagatc tttgtaatca acacaaaatg cgccttcttc
    21361 aaactttatc gttgaaaaag ttactttcaa atcagcatcg agagttttga caatatcccc
    21421 ttcaaatata tccacaccat tcttatcttt cattcctgtg gattgcatga gttcaatatc
    21481 aagaaaatca ataatttctt caaactgctt atcttcttct aaatcttccc tcaaaactaa
    21541 acaattgcca taaataaaat taggtaatgc ctcagcaatc accccattaa gatgattgct
    21601 taaaatatca ggttttaccc acgctctaaa cttcggtatc attcgctgtc ctcctcaaat
    21661 actcttctaa tatttcttta tacttctcta caaatttgaa acgatcttga tgaagtttct
    21721 tgctccaatt tgtttgccga tccagctcac gcatctgatc gaaccctttt tgaatttcgt
    21781 tgtaataaaa ttcaatgttt gctgctgctt tccaatgcct gctacttcgc actcctgctc
    21841 ctgtttcagc catttctaac ttaactaatt cagctcgttc ttttgatttt ttatctttct
    21901 gaatcttcat catgattttc ttgaggatga tgtcactgta ttgtgtaatg agatccatta
    21961 tttcccctcc acatacctaa actgtcgtcc ttttgaatca atccataagc tcctagctct
    22021 atcccagatg atgtttttgc tcagaccagt aatttcagat aactgttcag cggtacctgt
    22081 tactagaatt cgatcaccat gccagattgc aatttttctc ggcgttttcc gtttggtttt
    22141 ttcagcccac attgatttac cgagcttttg aacttctgca actatttctt tgtcttcttg
    22201 ccaagattct gacttggtta attcagcaat tcgtttcatt gtcgctttct tatccacgct
    22261 cattcctcca atctacgaat ttcccttctt aagttctcta tgtgcaaatc gattgccttt
    22321 ctcgccgttt cattgaccat cactgccttt gttcgttcta gatcgtcaat ttcacgctga
    22381 atgtttcgaa tacgcatttg aatcacttct tctgttgtca tgatggacca cctcgttaaa
    22441 aacgctcttc cttgaacgta ttccgatatt ttttagctaa tatcaacggc gcttgatatt
    22501 gatgacagaa caactttgcc ttgatcttaa agtcttttgt ctgcattcct ttgacatcta
    22561 cgactttgac aagtttgccg tttttataaa atgtgaagtc gggaatatac tcgatcttgc
    22621 gatacttctt tccgtctagt tcaaatttcg gcatcagctc aaatctttcc tgaagtttta
    22681 ctttccagcc atttgcttca gcttgccaca aggctagatc gtaatactct gcttctgcga
    22741 tagaatcaaa cttgatacct cgatgaacag tttttttatt acggtattta ttcatgcgat
    22801 actacctttc actggtttta tgcgcttgtc tgctgtttgt tggaatttca gcgcataacc
    22861 ttctgaattc ttaaatatcc tagaaacaat tctttcaccg taggcttctc ttagttcagg
    22921 accagataag tttgttgtga tgatcgttgc cttgttctgt ctggcttcta agagcgtgtt
    22981 taacgtgttg tttgtaaact gcctactatt tgatacccca ctacctaatt cagctccaat
    23041 atcgtcaaaa accaccaaat cagttgtttt aatatcggct ataagcgatc cttcaatttc
    23101 ttttctcagt tcagtattgt tataagaaaa ctttatttgc tctaataatt cttgatagct
    23161 tataaaaagt attttcttgt cataatttga gcgctcaagt atttcccaag ctgtggccat
    23221 tgataagtgg ctttttccgc ttcctgattt ccctgataga atgaaatgtg caggatggtt
    23281 cagcagaacc tcattcgtat agctttttgc tttttctaaa gcgatttttg tttcttgatc
    23341 cactacgtga taattttcca ttttgcattt aaacaaagtt ttatctgtta ataccgaacc
    23401 attttgaaaa aaactcaacg ctcgtgcttt taagctatcg ttatatatcc gttcggtctg
    23461 tatatcctct ttcacacgta acgctttata accacaactc atgcatgttg gtttacaacg
    23521 ttctgaacca tccttatttt tagctcgcca actatacaaa ggttcgctac attctggaca
    23581 ttttccgctt tgcactaata ctcttcttat tagtttctcc atagcatttg ctaggctttc
    23641 catgtgatgc atctcctttt taaattggca agtcgtcata ttcactagga ttgctgtact
    23701 gtagtttttg actttgcttt ttatgattct tcttgtctgc tttgatttcg aatttgagct
    23761 tctcaaattt ttctctcaat ttcttagcac ttctaatatt tccaaaccaa aattcatttg
    23821 taggtagcca attgatcaca tactcaatcg cttctataga tgctttatct ctttcttcca
    23881 tcaacctgat tgtgtctgcc catttttcga tatctacttt gttcatttct tttggaaaat
    23941 cttcagttaa attactttgc aactttttag caaggcgtaa gtgttcgtca gaatacttac
    24001 ctttcttttc ttctttatct atatctttat cttcttctat atctttatct gtaccgtcac
    24061 gtgacgtcac gctaacgtca ttttccaatt tgagacgttc ctgtctcttt ctttccctgt
    24121 atttacggtt tctttcagca ttttttagcc ttactttatc cataccctcg atattttgat
    24181 gtttttccca attactgatg gcaattagtc catcactgct tagatcaatc atgttgaaat
    24241 ttgccaatgt agttagcgct aagcgaaccg tatttacgtt tttgccaaac aatgtagcaa
    24301 gcatttcttc ggtataaggc atgttcctct ggatatatat cagaccatcg tcgttagtct
    24361 ttcctgctaa gactagtaat cgaatccata taacgatgat ggcatccgac tcaggaacag
    24421 cttggattaa tcgtattttt tcatcgtcaa acatagtagt tttaagtttg atccaactta
    24481 tctcagccaa atttatcctc ctatccttaa ctttttaatt gtttcctggt ttaacttgat
    24541 ccctttgatt tgatatttat ttttgaaatt gatcacacct attttgtgtt tctc
//
LOCUS       DACDIG010000040        24311 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.040, whole genome shotgun sequence.
ACCESSION   DACDIG010000040 DACDIG010000000
VERSION     DACDIG010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.040"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1153)
                     /locus_tag="IV247_001679"
     CDS             complement(<1..1153)
                     /locus_tag="IV247_001679"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAP8777751.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            1338..3779
                     /locus_tag="IV247_001680"
     CDS             1338..3779
                     /locus_tag="IV247_001680"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAP8777752.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(4109..4813)
                     /locus_tag="IV247_001681"
     CDS             complement(4109..4813)
                     /locus_tag="IV247_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777753.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(5000..5245)
                     /locus_tag="IV247_001682"
     CDS             complement(5000..5245)
                     /locus_tag="IV247_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777754.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(5262..6113)
                     /locus_tag="IV247_001683"
     CDS             complement(5262..6113)
                     /locus_tag="IV247_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP8777755.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            6279..7283
                     /locus_tag="IV247_001684"
     CDS             6279..7283
                     /locus_tag="IV247_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAP8777756.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(7524..7880)
                     /locus_tag="IV247_001685"
     CDS             complement(7524..7880)
                     /locus_tag="IV247_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP8777757.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            8248..9435
                     /gene="tuf"
                     /locus_tag="IV247_001686"
     CDS             8248..9435
                     /gene="tuf"
                     /locus_tag="IV247_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP8777758.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(9623..12214)
                     /gene="mprF"
                     /locus_tag="IV247_001687"
     CDS             complement(9623..12214)
                     /gene="mprF"
                     /locus_tag="IV247_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP8777759.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(12240..12959)
                     /locus_tag="IV247_001688"
     CDS             complement(12240..12959)
                     /locus_tag="IV247_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8777760.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            13279..13800
                     /locus_tag="IV247_001689"
     CDS             13279..13800
                     /locus_tag="IV247_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAP8777761.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            13859..14719
                     /locus_tag="IV247_001690"
     CDS             13859..14719
                     /locus_tag="IV247_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAP8777762.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLCGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            14826..17219
                     /locus_tag="IV247_001691"
     CDS             14826..17219
                     /locus_tag="IV247_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP8777763.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            17401..19242
                     /locus_tag="IV247_001692"
     CDS             17401..19242
                     /locus_tag="IV247_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAP8777764.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            19266..20375
                     /gene="rpoD"
                     /locus_tag="IV247_001693"
     CDS             19266..20375
                     /gene="rpoD"
                     /locus_tag="IV247_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAP8777765.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            20595..22694
                     /locus_tag="IV247_001694"
     CDS             20595..22694
                     /locus_tag="IV247_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP8777766.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            22890..23753
                     /locus_tag="IV247_001695"
     CDS             22890..23753
                     /locus_tag="IV247_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP8777767.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            23858..>24311
                     /locus_tag="IV247_001696"
     CDS             23858..>24311
                     /locus_tag="IV247_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP8777768.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKE"
BASE COUNT         8127 a         4299 c         5037 g         6848 t
ORIGIN      
        1 atattttgta gtaacgaata tcattttcga tctttgtaat cgaaaaaggt acatctgaag
       61 tatattctgc acattctccg tgttcaggaa gcgtctcact gaaaatcgtt tcatattcgt
      121 caacagtgat ccgtgttcga gaatctagca tctcttgatg agattgtgcg aagagatatt
      181 cttgatagtt ttcttccaga taacctgtaa aaaattcact tactgcacca gaaccgtagc
      241 tgaatagacc gatgcgatct cctggctgta gtgatttcga attttccagt agagaagtta
      301 gccccaggta taatgaacca gtgtaaagat taccgattcg tcggctgtaa cggatgcttt
      361 cttcatagcg agccataaga cgttcctgat tttcttcgtc tgtttggtct aatacgcttt
      421 gcaatgcctt ttttcccatc ttagtatacg gaatatggaa agcaatcgcc tgataatctt
      481 cgagtcctcg acccgacaat tctttatgtc gattccaaac tttttggaat gattcgatat
      541 acgtagaatt agataaagga ccatcaacaa caggaaattc gctataatct ggacgccaga
      601 aatcatagat atcttctgtc agaaatacgc tgtcgtcttc aatcgataaa atacgcgggt
      661 tttgagtgat catcatcgca acagcaccga caccttgcgt cacttcacca ccgcttgcca
      721 agccgtaacg agcaatatca cttgctatga ctagtacttt tcgttctgga tgatttttga
      781 catattcttt cgccatgtgc aaagctgcgg tgccaccgta gcaagcctct tttacttcaa
      841 aagaacgagc aaaagattgg attttcagta aatggtggat aattaccgca ctcgcttttg
      901 aatgatcaat cccggattct gttccgacga tgaccatgtc gattagctca cggtcttttt
      961 ctgttacgat tttgctggca gcatttgctc ctagtgtaat gatgtcttca tttgcacgat
     1021 tgactgccat ttggtcttga ccaatcccta tatggtattt tgcaggatca tccccacggc
     1081 tttctgccaa ctctgtcata tctaaatata aattaggaat aaaaaaggaa agacgatcaa
     1141 tccctatttt catttcaaca cctctttact tatgataaaa aacttacttt actaaggtat
     1201 cataaaaaga ttaaaagaaa tataaaaaag agaatattta aggattgtta aaatttaatc
     1261 ttagaaaaaa ataactggaa caagagctat ttgtttttac agcaaagaat ggtatatttt
     1321 tgtaggaggt ggcttacttg aaagaagtcg ttatgataga tgctgcaaga acgccgatag
     1381 gaaagtatcg cggaagttta agtccattta ctgcagttga attaggcaca cttgtgacta
     1441 aaggattatt agataaaaca aaactgaaaa aagataaaat cgatcaagta atatttggaa
     1501 atgtcctgca agctggtaat ggtcaaaatg ttgccagaca gattgctttg aacagcggtc
     1561 tccctgtaga tgtcccagca atgacgatca atgaagtttg cggatctggt atgaaagcag
     1621 tgatcttagc acgccagctg atccaattag gtgaagcaga acttgtgata gccggcggaa
     1681 cagagagtat gtctcaagca cctatgctga aacagtatca gtcagaaaca aatgaatatg
     1741 gtgaaccaat ttccagtatg gtcaacgacg gattgactga cgcattttca aatgcacata
     1801 tgggattaac cgcagagaat gttgcaacac aattttctgt gagcagagaa gagcaggatc
     1861 gctatgcctt gtcgtcccag ttgaaagcag cacatgctgt cgaagccggt gtattttctg
     1921 aggagatcat cccagtcaag atttctgatg aagacgtgtt atctgaggat gaagcagttc
     1981 gtggaaatag tacattggaa aaactgggca cgttacgtac agtattctca gaagaaggaa
     2041 ctgtaacagc aggaaatgct tccccgttga atgacggtgc ctctgtggtg atccttgcat
     2101 ccaaagaata cgcagaaaat aataatctgc cttatttagc aaccatcaaa gaagtagcgg
     2161 aggtcggtat tgacccaagt atcatgggaa tcgctccaat caaagccatt caaaaattga
     2221 cagatagatc aggaatgaat ttatcaacaa tcgatctttt tgagatcaat gaagcatttg
     2281 cagcttcttc tatcgtagtt agccaagaac tgcagttaga tgaagaaaag gtcaatatat
     2341 atggaggagc aatcgcatta ggccatccaa ttggtgcaag cggtgcacgt atcttgacta
     2401 cattagctta cggattgctt cgtgaacaga agagatacgg tattgcttca ttatgtattg
     2461 gtggaggttt aggcttggca gtcttgttag aagcgaatat ggagcaaaca cataaggatg
     2521 tgcaaaaaaa aaagttttat cagcttacgc catctgaacg gcgcagtcaa ttaatagaaa
     2581 aaaatgtatt gacacaagaa actgcgttga tttttcagga acaaacactt tctgaagagc
     2641 tctccgatca catgatcgaa aaccaagtga gtgaagtgga aatcccaatg gggatcgcgc
     2701 agaattttca aatcaatggc aagaaaaaat ggattccgat ggcaacagag gaaccatctg
     2761 tcattgcagc tgcaagtaat ggtgcaaaaa tctgtggaaa tatctgcgca gaaacacctc
     2821 agcggctgat gcgagggcaa atcgttctat caggaaaatc ggagtatcaa gcagtgatca
     2881 atgcagttaa tcatcgaaaa gaagagctga ttctttgtgc aaatgaaagt tatccttcaa
     2941 tcgtgaaacg cgggggcggt gttcaagata tctctactcg ggaattcatg gggagtttcc
     3001 atgcatactt atctattgat tttttagtgg atgtaaaaga tgcaatgggc gctaacatga
     3061 tcaactcgat tcttgagagt gtggcaaaca agttacggga gtggttccct gaagaagaga
     3121 ttctattcag tattttaagt aattttgcta cagaatcatt ggcttctgcc tgttgtgaaa
     3181 tcccattcga acgcttaggg agaaacaaag aaatcggtga acaaattgcc aaaaaaatcc
     3241 aacaggcagg ggaatatgcg aagcttgacc cttaccgtgc agcaacgcac aacaaaggga
     3301 tcatgaatgg tatagaagct gtagtggcag ctacaggaaa tgatacacgt gcggttagcg
     3361 cttcgatcca cgcctacgcg gcaagaaatg gattatacca aggattaaca gactggcaga
     3421 tcaaagggga taaactagtt ggaaaactaa cggtgccttt ggctgttgcc acagtaggcg
     3481 gagcttccaa tatcctgcca aaagcaaaag catctttagc tatgttagat attgattcag
     3541 ccaaggaact agcacaagta attgctgcag tagggttagc tcaaaacctg gctgctttgc
     3601 gtgcattagt tacagaagga atccaaaaag gccacatggg acttcaagct cgttcattag
     3661 cgatctcaat tggggctatt ggtgaggaaa tcgagcaagt tgcgaaaaaa ttacgtgaag
     3721 cagaaaagat gaaccagcag acagctatac aaatattgga aaaaatccgg gaaaaataaa
     3781 aaagcaatat ctttagaaga ataaagcaag atttacagtt attttattta ctgcagatct
     3841 tgcttttttc tctatcagaa aatatgttta ttattagaat tttttatagt taaggttagg
     3901 catcttaaga aatcaggcaa ctccaagata tcttcaggta gaagagaagt cgcctaacat
     3961 tgtaggatca taggtgattt gttggacagg tcaagctgat atttgagcct gatgttagaa
     4021 ggaatttctt tttacttgct gtagaaatta ttatatgact aaaaaggctg ggattttaaa
     4081 tcccagcctt ttatgacaaa tagaattttt atcgatcagt aatcgctgtc gctgcaaatt
     4141 gttcgatcaa aggaatcaaa gtcaatttga ctttgtattt ccgttcgagt gctttttcga
     4201 taacgaactc attttcagta gtcatagaaa tagttgctaa tttacgaggt tcatcagcat
     4261 ttttttcatt aataactact tcatgttcat tttgttctgt gtaaaattta ggatactcag
     4321 caacggcttt tgaaaaatca gcaaaactta aagcggcatt cgtatcagtt gagcgtgtat
     4381 tttcagttgt gtccgctttt ggtttttccg ttttttcttc tggcacactg actgtttctt
     4441 ctgtaactgg tgtcgcaaca gcttgcgcag ttggttcatc gtttttcaat cgttgatcat
     4501 tccagtaaga cacgatttca ataagaagat tgtcacggaa tcgttcgaaa atcccgtttt
     4561 ctcctgaacg aattctcttt aatacagtat tgaagttgtc atcgattggc acgttgatcc
     4621 gtgtatcttc atttgttttc ccagctaatc cgatcgttaa gatttgacgg tttttgggat
     4681 caagcatgac ttgtaacggc aaaccataac gtcgcaagct ctcttcttct agagaacgga
     4741 ataattgtcc tcttacacca tccaatgaag gacggaattg atttaataat ttttgtttct
     4801 gttttttctt catatgttct ctccttcgct catacccttt cgattataag aaaaaaacag
     4861 ccaaatgaaa aggagtaatg caaaaaaact tgcgtatacg caaattacaa cagaaaaatt
     4921 tgcgtatttt cccgtttttt gaaagaaaaa taaaaaatgg aacggattat gtatatatac
     4981 ataatccgtt ccattctttc tattttttcg gtatcaaggc aaaaacaggt agttcaggtg
     5041 aataaatgac ttcatgttcg tgccagggtt tgaccattag cgaatgatcg aaccgttttt
     5101 tcagttcttc gatgatttga tcatgtgtcc agtttcttgc aggaaaatgt tggattttct
     5161 ctgcatcgat caaaaagaca aaatcggaat gaaaagtctg ggtaacttct tccgagagag
     5221 aattcaagcg ttcgattgga tccataaaat cacctttcga cttaaaattg ggtagtatct
     5281 gggtcttttg ctttgccgac tacaccatct aactgatcga tttgtttcat ttcttcttct
     5341 gttaattcaa aatcaaataa ttcagtattt tctttgattc tgcttggtgt cactgactta
     5401 ggaagaggaa ggaattcgtg ttgcagcgac caacgcaaag caacttgtgc aatcgtcttg
     5461 tcatgtgctt cagcgatttg tttcatttca ggcacatcga agattttacc agtgcctaat
     5521 ggactatatg cttcgaggat catatcatgt ttttttgcat actcaactac agttgtctgt
     5581 aattcgcctg gtgcaaggaa aacttgattg accatcggca taatttttgc tgtttttgct
     5641 agtgtatcta gatggtgggg taagaaattg ctcacaccga ttgctttgat tttacctgct
     5701 tcatacagtt cttcaaatgc tcgccatgta tctgcatttg cttgttccca attatcacga
     5761 aaagcaacag ggtttggcca gtggatcaaa aacagatcta aataatctgt ttggagcttc
     5821 ttcaatgatt cttcaaaaga agacatcaca agttcatagc tgtggtttgc attccataat
     5881 tttgttgtca agaatatctc ttctctagga attccacttt ccttaatcgc ttggccgacg
     5941 ctttcttcat tcttgtatcc ttgggcagta tcgatatggc gataacctgc agccagcgct
     6001 tctttaacag aggaaacagc gacatcccca tcaggtgttt gccatgtgcc gaatcctact
     6061 accgggattt cgtaaccatt tgatagacga taagtgcttg ttaatgaatt catactttca
     6121 gctccttttt tattcgttca actataagtg tagcgcattt ttcgaaaaaa agaatccatt
     6181 ttgtttctac tcttgaaatt tttgtttaaa agaaaatgat tttgattctg gtatttttga
     6241 ttttttttcc gtaaactaag acagaaggga atgagaaaat ggatatctat ttaaaaaaag
     6301 caattctgca tattattgac agagaaactg gttctccagt cttttcacaa aaagagctgg
     6361 atctgacaaa agaatatatt cgtgattttc ttcaaaagaa gatccaaaaa atctcttctg
     6421 ctcaaacaaa aactggacaa ctagcagaag aaagcatgat cgttgaatca cttaaaatcg
     6481 ctcaagaaaa ttttgtggaa gcaagtgaag caatcgttca gcggtggttc gatatctacc
     6541 aagaaagtga agaagcacct agtgcagatg tatttgttgt cttgtatgaa ttagatacag
     6601 tgatgtactt atctttattg aaagtcaatt atcgtgaggc ttacactcac tttgttgaag
     6661 cagacgaagc agggatcgac aaccgcttga ttttgaatcg agcaattcta ggggcgaaaa
     6721 cacaaaaagc agatgaagca ttcgctgtga atttgactga ttggacctac gaattaattg
     6781 aaaaaaaata tgagttttct ggcaaaaaag agctttattt ttctggaaga gtgatcgaaa
     6841 gtgttcctgc accttcttta gaagaaaatg taaaagtgat ccaaaaagta gccaagcatc
     6901 ttggaaaaaa atttgagaca gaagaatttg atatcatggc tgatttgaaa gaagctgtct
     6961 atgatacgat agaagaaaaa ggtcggttgg atcatgagat gattgcggaa aaagtcttca
     7021 aggaaaacat cacggcaaaa atggctttta aagaagaagt acaagagcaa ggatttgtcc
     7081 cagaagcacc tcctgtaaaa gaagtacaag aaatttctga gaaaaaattt ggaaaacaaa
     7141 aattcaagct atcaaacggg atcgaactga tcgtaccagt agatgtatat cgcaacccag
     7201 atttgattga atttgtgaat aatccggatg gtacgatatc cgtgatgatc aaaaatgtgg
     7261 atgaagtatt gaatcgttta taaaaatgac tagaatatta ggtgttcaag taccagtgat
     7321 cagttaaatg aattgtgtca agagaaggat cggaaaagac gaatataaca aaaaaagagc
     7381 gtgagacaaa agtaaagaac acttttgtct cacgctctaa acacgtataa acggcggaag
     7441 cagaagcaac tccttcttat ttcttggagt taaacgcttc tgtcccagcc tctttttatc
     7501 taataaaatt ttaagaccat ttactaatta tcgatttttt ctgccgcatt tttaggcact
     7561 tcagaagcct ttatttcttc ccagcttaat acacctttac gaggattgac tttgagttta
     7621 agatacgcat tcatctttaa tggatgttct ctttcttcgc gaagcgtcac ctgttttttt
     7681 tcaccatctt tattataagc cgcttgcttg taggtataag tcgtgaattt ctctccgtta
     7741 tctgcttggg tagctgacat ttcgccattc gtcgtgattt ttgtataata agcttcgccg
     7801 ccgtaaaaat aatgataagc tgcaaaactt ccgccgagaa atatgcctaa aacgacaagt
     7861 acgccaatta attttttcat gtacttcacc tctttcctta ttattaacga aatagaagat
     7921 ttcgtctatt gaaagttgtt ggcaacagct gaattttaaa cttttcctca ttttgttctt
     7981 attgcttcaa aagaaagtta aggactatct tgaagttttt ctcaaaaaac acattttttt
     8041 atttataaaa ggtacttttt tcgcgtataa gacagacgag tgctcttagt tgactttgac
     8101 caatattgac gtataataag cttagttcga atcttacaaa aagtaagtcg gacgtattct
     8161 ttttgattta gtcaaaaaaa gactataata aaaaggtgag agctgaaatt tttcagtgat
     8221 ctatattatt taggaggttt gtaaattatg gcaaaagaac attatgacag aagtaaacca
     8281 cacgttaaca ttggtacgat tggacacgtt gaccatggga aaactacttt aactgctgcg
     8341 atcactacag tattaggcaa aaaaggccta gcaaatcctc aagactatgc tagtattgat
     8401 gcagctccag aagaacgtga acgtgggatt acgatcaata ccgcacacgt agaatatgaa
     8461 acagaaaaac gccactacgc gcatattgat gcaccaggac acgcggacta tgtgaaaaac
     8521 atgattacag gggcagcaca aatggatggt gcaatcttag ttgtttctgc aactgacggt
     8581 ccgatgcctc aaacacgtga acacatttta ttgtcacgcc aagttggtgt aaaatacctg
     8641 attgttttct tgaacaaagt tgatttagtc gatgatgaag aattgatcga tttggtagaa
     8701 atggaagttc gcgagttatt gagcgaatat ggtttcccag gcgatgacac tcctgtgatc
     8761 aaaggttccg cattaaaagc attgcaaggc gatccagatg ctgaagctgc tattatggaa
     8821 ttgatggata cagtagatga atatatccca acaccagaac gtgatacaga taaaccatta
     8881 ctattgccag tggaagacgt cttctcaatt acaggtcgag gaactgttgc ctcaggtcgt
     8941 attgatcgtg gtgctgttcg tgtcggtgat gaggtagaga tcgtagggat caaacctgaa
     9001 acacaaaaag cagttgtaac aggtgtagaa atgttccgta aaacgttaga ttacggggaa
     9061 gctggggata acgtaggcgt gttgttacgg gggatccaac gtgacgatat cgaacgtgga
     9121 caagtacttg ctaaaccagg ttccattact ccacatacaa aattcaaagc agaagtgtac
     9181 gtgttgacaa aagaagaagg tggacgtcat actccattct tcaacaacta tcgtccacag
     9241 ttctacttcc gcacaactga tgttacagga acaatcacat tgccagaaga tacagaaatg
     9301 gtcatgcctg gtgacaacgt gacgattgat gttgacttga tccatccaat cgctgttgaa
     9361 aacggaacga cattctcaat tcgtgaaggc ggacggacag taggttcagg tatcgttaca
     9421 gagatcgaag cataagaaac gcgactcgaa tattttggta atgaaaaatg tttaaaataa
     9481 aagagcggtc caaacaggat ttctgtttgg accgctcttt gttcttcaat tgtataagaa
     9541 gagattgtag aagcatgaac tttcgtttta cttcagtctc ttttattcat catgagagtg
     9601 ttcatctatc ggtatgcagt ttttaatact ttcttcgtat ccattttttc aaaccactta
     9661 ttctaccttg tgtctgctca actggcgcat tatcaataat cagcaaagca atcataacat
     9721 atgcaatcca actatctcta gaatatagag tataacgtga gacccagttt gtagcgtact
     9781 tgcttttata ttctcttaat ccatggaaag aataaaaatg agaaccaaac tcgtaaacca
     9841 agtatgcaat cctttcctgt aaaaaactct ttctggaagt tccgacattt gaaagagggg
     9901 ccatccctaa attgaaccat tggatatgtt cttctttcat ataggcaaac aaatgcaaga
     9961 acaagaaatc catactgcca gaaggtgctt ttttagaatc atatcgcatc agatcaatcg
    10021 ttcctacttt attgttcgtg taagtaggca tgatcgttgc aaaagcgaca atttcttctt
    10081 gggtattttt gacaactgca ataggcgctc tttgtagata atcttcagag aaaaagccta
    10141 aagaaaaccc tttttccttt cgtgaaccca accaattatc agagatattt ttgaaagtac
    10201 tcatttgctc agtagaaaat ggtggttgta agacatcaaa tgtaaatcct tctcgttcga
    10261 tccgattcaa gacagcacga gtgcccttca tttttttccc tgaagttgta aaactattca
    10321 ggtctacata agcttcttcc cccatcttaa tgaaatcgta gccgaattca tgcaaaatca
    10381 taacaatctc ttcgcttgtt tcgtagaaaa ctggtaaata gcatagtcga tctgtttctt
    10441 cgataaaagc ttcgattgct tctggaaaat cttctttctt accagaagga tcacccatta
    10501 caatacattt attattgaag caagcgaatt ggagaagaac agttggttca ccgtcttttt
    10561 cataggcaaa catccgctta tcttttaaga agatcaattg actatcggag tttcctccat
    10621 aagttgtaag gatcttcagg gccttttctt cattaaaggc ttcgccaatc tgtttctttt
    10681 ctccttgaag gaaatgaaca aatagaacga tcatgaaact aacagcgatg atggccaaca
    10741 atcccgaaaa ccaaatcttt tcagaaggaa ataagaaaaa ggaaataaaa tggtgacgcc
    10801 gatgagggaa atccggcaga ttgtataccc caataacgat gtaaagcaag gacagtgcac
    10861 caataatgat tccatcgatc gtacgccact cccatgaata gatcaattgt tcccgaaata
    10921 attcgttttt agaagcaaga atgatcagaa gcagaataga caagaaaacc accggtgtga
    10981 aactaaaatc acttaataaa acataaagta aagctagaac aatcaagaag attgtaggca
    11041 aatatgctcg tttcacacgt gctgcgatcc ctctgcccat aatgattaaa agaaatccca
    11101 atagtatact aggaaattgg ataataaaat gaaatttcaa tgggttcaaa ctatgaagcc
    11161 agcgaaactc tgtaaaagct tgcgggatag tggcagaaag tactagcatg attccagaaa
    11221 agtataataa gacaaccacg attttatgcg caatttctgt tgctaattgt ttagggatac
    11281 cagaataacg ctgatcaaaa gtggaaccga tatttttgaa gaaaaagacg attccaatca
    11341 aaaacggaat gatgtagtag aacaggcgat agagcaagat ccacacaacg actatttctc
    11401 ttggcacacc aatagcggac aacccgataa tcatcattac atcgaaactc ccaatttctc
    11461 ctggaatcat agacacgatt cctatcactg aagcagcaat gaataatgga attgtttgcc
    11521 acagatcaat ccggatttcc attaaataac caacagaaag gaaactaaac aggacaccgg
    11581 tccattccaa caatgaaact agcaatagct gtccttgagt tttaagtgag gtaccaccaa
    11641 ggaatccttt ctttttgaaa gtagtaaata aaaagacgac aggaaaatat agaccgccgc
    11701 cgatcaacca tatccaatat tgcttcaaaa aaggagcaac tggcgtgaag actacaagta
    11761 aaaaagaaag cagactgtaa atagaaagtc cagccatcaa aaacaagaaa actttcgata
    11821 atgcttggat cacgtttctg ctctctttct tttgtccata tagttcagag cgaagcccca
    11881 cactaacaaa cccgccgaaa ccggcaatat tgttgatcgt attgatcagc cagcttgtct
    11941 caaacaggta acgaggattc tgcttttgtt caagcatccg attcaaaata atatcataac
    12001 caaccatagg taacacagag ataagaccaa tcaatagcat tagtccgctt ttccatacag
    12061 gtatagtggc aaacgtttct cctaattgct ggatagatag agttttaccg atggacatca
    12121 attcaccgac aataatgaca atgacagaaa tcaaaaagat tgttttgaac agtcctaggt
    12181 ggtttttcag ccattgtatg gtatttttta acaaataaat caccttctat tcctaatagt
    12241 tatgtttcag aaagaaattg aagaacttct cgattgaagc gttcaggatc ttttctagct
    12301 aattcatgtc cttgttcttt cacaagcaca aaggaagctt ttggtatagt tttagctata
    12361 tacaaagaat gttttagctt gattacatct ttttttccca cgatgatcaa agtgggggag
    12421 ttgatttttt ttaaatcatt ttctgtaagc ccgatgtcat gcagcaaaag tttgataact
    12481 aaaagatttt tgcgaagact tgggcgaaat aaagagagta accataccca tgcataatga
    12541 atattactga tgactctggc agaaaaacga acgcctttaa caagcgtgtt tccagaattt
    12601 aaaatcaatc gatgaacttt tttagggaag ctgctggcaa ataccagtgc tagatttgct
    12661 ccatcactaa atccaagaaa atctgcctga tcgatttttt ccagcaacat gatcgtattc
    12721 agatcctcgg ccattaactg aaaattcaac atagatgctt cgtttgtcga acgaccatgt
    12781 cctcggctat ctactagata cacggtatag tagcgttcga gtacagggac ctgttctgaa
    12841 aaaaagcggc cgctgccgtc attgccatgc agtaaaaaga gagggaagcc ttgaccgctc
    12901 ttttcatagt aaatttcgct cccatctggc atttttgcat atttcttttc ttttttcaca
    12961 taatcaccta ctgtgtaaag tataacgatt atatcagtca ttgggcaatt gtttttactg
    13021 aaaaacaacg atcattttcg taagatgtca aaaaatacag gaaaattcat tttttcttaa
    13081 ggcgtacaca gaaatcaagg aagaaatagc caagaagata aagaaatagt aagataaaaa
    13141 aagatgtcaa aataactagg tgatgaaatg aagcgaaaac gattatctat tatccgtatt
    13201 cttttttaca tgatcattct gacggttatt ggtgcggtgt tgtttttatg aggaagaact
    13261 tatcgttcgg taaaacgagt ggaaagctat caaatagaaa taacaacagc tgcaaaaaat
    13321 tatcatatgg aaaactacga gaatttgatc aaggcgataa tattgaccga atcaaaagga
    13381 aaagggacag atctcatgca gagctcggaa agtctgcatg gagaagctga gatcattgat
    13441 aatccagctg acagtattga tcaaggtgtc aaatatttgg cggaaatgat agtagaagca
    13501 aagaaacaag gatgcgattt atggacagct gtccaagcat ataattttgg gaaagattat
    13561 attgcctatg taaaaaatca tggcggagaa aacacagtag aattagcaga agcgtattct
    13621 aaagatgtgc tgagcccact tttaggaaat gaggaaggga aacaataccg atattggcgg
    13681 atccagtcct tgctttacaa tggcggctat ctctatcata atggaggtaa catgttttat
    13741 cctgatattg tcaaaatgaa tgaaaaaata atagagacct atgaaaaagt ctttaagtaa
    13801 aatagcagga aatgttcaat catgaataaa attaaatgaa agaaggagtg cgcaaatagt
    13861 ggaaaaaagg caaaaaatgg aaaatcaaga agtggggtac acaaccaaag actggatgtt
    13921 acggtttgtg aagggaatgt ttatcggttc aggatttatc ttgccaggag tcagcggtgg
    13981 tgcattagca gctgtctttg gcatgtatga gcggctgatt tcttttttag cccaccttac
    14041 aaaaaatttc aaagaaaatg ttttgttttt tctgcctgtt ggtctaggcg gagtgacagg
    14101 catctttcta ctttcttttt tagtcagttt tttattaggt gcgtatgaaa caacgatatt
    14161 atggtttttt gtcggatgta tcgctggaac tgttcctgcg ttatggaaag aagcagggaa
    14221 aaaaggaaga gatacttctg atgtgatcat catggttgtt acttttgttc tggcttattt
    14281 gtttttatta tgcggcgctc aattatttga cgggcaagtc gatcagaatt ttggaacatg
    14341 gctgattgcc ggcggattga ttggtttggg tatgatcgtc ccaggattga gcccttctaa
    14401 ttttttggtg tatatgggat tgtataaagc gatggctgac ggatttaaag aagtacgact
    14461 tgaagtgatt gtgccgatcg gaattggtgg gattctttgt gttcttggat tgtcaaaaat
    14521 catggattac attttctcaa aggcatatag caagctattc catttcattt taggtgtagt
    14581 ttttgcttca acgattatga tcattcctac cgattacgcg ggattgggag ttgctggggt
    14641 actagcttgt cctgttcttt ttatagccgg tacagcacta ggttggtgga tgagccgttt
    14701 ggaggaacaa tataagtaaa gatagattga taataaaaac agcacacggg tgattcaaac
    14761 aaaaaggttg gcatcttttt tgtaaaatca ttcgtgtgtt tttctataaa ttttataaaa
    14821 acaagttgtt tttaatcttg gaacacgcta gacttaaaca cgtgattatt tttgaaagtt
    14881 ggcggaaaat ggagaaaaag aaaatgaatg gacttacaca gcaagaggta gttcagaaaa
    14941 tggcttctgg ccagcaaaat gactaccagg aagatgcatc aaaaagtaca aaacaaatat
    15001 tcagcgataa tatattgacg ttattcaatt ttctgaactt tggtattggg atttgtctgt
    15061 tatttgtagg tgcgtattcc aacatggcgt atttagcaat tattgtcgtc aatatcgtta
    15121 ttggaattta tcaagaaata catgcaagaa atcttgtacg gaaattatcg attgtttccc
    15181 aatcagaagt actagtgatc cgggacggta aagaacaatc gatttctact aaagagctgg
    15241 tattaggaga tttggtaaaa atcggagcag gagaacagat accagccgat ttacaggtta
    15301 tatctggacg ggcagaagca aatgaagctc ttttgacagg cgaatctgat ttgattgtca
    15361 aagaaactgg agctgaattg ctttctggaa gctttttgac aagcggttct gtcatggctg
    15421 aagttatccg agtaggtgca gataattatg ctgtccgctt gactagcgag gcaaagactc
    15481 acaaagctat tcgttctgaa cttgtagatt cgattcgcaa agtatccaaa ttcactagtt
    15541 tcgtgattat tcctttgggg atcatactat ttttggaagc actgttcatc cgcgatgctt
    15601 ctatcaaaat ttctgttatt gcatctgcag cagctttgtt aggcatgctt ccaaagggtt
    15661 tggtattgct aataagtatt gcattaacga caggggtaac caaactggca aaaaaacgaa
    15721 tccttgttca agacatgtat tcaattgaaa cgctggccca tgtcgataca ttatgcttgg
    15781 ataaaacagg gaccattact gaagggaaaa tgagtatcca gcgggtagaa gcactacatc
    15841 cgctgtacga gaaagccatc ccagaaatta tggggacgta tctggcagaa agcagtgata
    15901 acaacatcac tatgcgggct atgagagatt attttgagct ttctaatcgg tatgatgtga
    15961 caaacaaagt cgcattttct tctgatcgca aatggggcgc gatggaattt ccagaactgg
    16021 gagtcgtgta cgtaggagcg cctgaacgtt tggtggaaga agatcggctt ccggacagtc
    16081 taaaaacagc ccaagcgaat ggctatcgcg tacttatgtt ggggatcgct ccaaatcagc
    16141 aattgaacga agaaaaacca gcatttgttg agccggttgc tctgtttgaa atcgatgatc
    16201 caatccgaaa aaacgctcaa gaaacactag cttatcttca tgaggaaggt gtggatctga
    16261 aagtgatttc tggagacaat ccttttactg tctccaatat cgctagacgt gcaggattac
    16321 cgaattatga tgcgtatgtc gatctttctc aaattactga agaaatggaa gtgcgagaag
    16381 ctgctcatcg ctatacggtt tttggacgag tgagtccgca gcaaaagaaa ttgctggtaa
    16441 atgaattgaa agaaagcggc agaacggttg caatgacagg cgacggtgtg aatgatgttt
    16501 tggcgttaag agaagcagat tgcagcattg ctatggcaga aggtgatgga gcaacacgtc
    16561 agattgcgaa tctggtattg ttggactctg attttactac attgccagaa gtcctgtttg
    16621 aaggacgccg agtggtcaac aatgtgacga aagtctcagg catctttttt atcaaaacga
    16681 tttattcatt tattttatcg attatatgtg ccgttacagc aattggattt ccgtttatcc
    16741 ctatccaaat cacgctgatt gatttagcga ttgaagggta tccttccttc tttttatctt
    16801 ttgaacaaga taaaagaaaa atcacttacc gttatttgcc tacagcactt attaatgctt
    16861 taccaaatgc cttgcttgta gtagtgaata tcattgcggt gtatctactt ggaaaaacat
    16921 tcgcatgggg aaatgctgaa actactactt tgatgtatta cttgctgatt gggatcagtt
    16981 gcatggcagt tgtaaaagct tgttatccct tcaacccatt gcgcatcttc ctagcagtaa
    17041 cgacgatcgt agggatatat gtagctgcaa tgctgttcca tggaattttg gaagtaaact
    17101 tagggttggg agagagcgta ggatacttct ttgtattcat gctcgtaaat attgttcttc
    17161 gagtattgta ctggaaggct gatattcaaa atcagttact gaaaagagta gcaaattgat
    17221 gcttaatgag atagaaatgt gttatacttg aaaaagcaat gaaatatgat acaatcaaac
    17281 atgtataatt gtgtccttta aaatcgcact gatttcagtt agtgcgattt tttaccggtt
    17341 attctcctga aatatcttct gttaaaccca aaagatatca atagagcagg gggtgaaaaa
    17401 atggctcaac ggattcctca agaggttatc gaaacagtac gcagtcaaac gaatatcgtt
    17461 gaggtgattg gacaatatgt ccagttgaaa aagtctggta aaaattactt ggggctgtgt
    17521 ccttttcatg aagaacgtac accgtctttt tctgttgcag aagacaagca gatttttcat
    17581 tgctttggtt gtggtaaagg cggaaacgtg tttacttttc ttcaagaact agaaggtcta
    17641 agttttccag aagcagtggt aaaagtggca gacttcgaac agataccgtt agaagatcaa
    17701 tggcgtcagc gtactatagc tgtccaaaat cctgaatctg ctacggggaa actgattgct
    17761 gctcatgaaa aagcagcgga aatctatcat cacatgctat tgcatacaaa agctggacag
    17821 ccagctttag aatatttgca aagtcgtggg ttgtctttag aattaattga agaattcaac
    17881 ataggttttg cacctaatga gcgttctttt cttcagcaag tattcaagaa tgaatcaatt
    17941 tcaaatgaat tgttagaacg ctctggattg tttgtcacgc atgatgatgg ccgtatgtct
    18001 gatcgttttt atcaaagagt gatgtttcca cttcgtaatt ctcaaggcca gacgatcggt
    18061 ttttcaggac gctggcttgc acctgaaaaa gggcaggaaa aagatcagcc caaatacttg
    18121 aatagtccag aaacagaact gttcaataaa agacaagttt tatttaatct tgataaagca
    18181 agaagcgata tccggaaaaa cggagaaatt ttgttgttcg aagggtttat ggatgtgatt
    18241 gctgcatggc aggctggtat caaaactgga ttagcttcta tggggacaag tctaacagct
    18301 caacagatcc aacaaatgga aaagctgacg aaagaattgg tcttttgcta tgacggagac
    18361 aacgcaggtt ttgaagcaac caatcgaggg attgaattgc ttcggcaaaa cagtcgatta
    18421 gcactttctg tcgtagtcgt tccagaaaag cttgatcctg atgaatatct ccgtaagtat
    18481 ggagaagcat ctttccaaga attagtggct catgggcgcg aaacagtctt tacttttaaa
    18541 tcacgttatt taaaacaagg gaaaaattta gataatgaaa aagacaagat cgaatacctc
    18601 gaagaattac tagttgaact tagccgagtt gtttcaccaa tcgaacaaga tatgtatcta
    18661 tctcaactgt caacagagtt tcagatcggc agagagacga tacaaaaaca actgcgcgat
    18721 cttcgaagga caaatcagca aaatcgtcaa gaacctcagg aaagtcaaca cactcaggtt
    18781 acgagacaag aaactgcacg gaaaaagaga cctttgacgc aagtagaaaa ggcagagcag
    18841 attctattat atcgtgcatt taaagaagtt tctgtccgta atattttaaa agaacgagaa
    18901 tttcagttta ttcatgatgc atatgcagaa gtttattttt tattcgatgc atatgtctca
    18961 caacattatg aattcaacct tgcagagttt ttagactttt tgcaagatga aaatttaagg
    19021 cggttggtgg tggaaatcga atatttaccg gtggcagaag aaagttcgcc acgagaaatc
    19081 caagacttgc tagatgtgat cagcaaatct ggcttagctg atgaaataac attaaaaaaa
    19141 caaatgcagc aagaagccag acgaactgga aacaaacagc ttgagcttga attgacgatt
    19201 gaaatcatca atttaacgaa acagttaaaa caagccaaat agctactcaa ggggggcctt
    19261 cttttatggc aaatggaaca gataaaaaaa cgtatgaagc agcagtagca gctttcatca
    19321 aagaaaacaa acccaaacga caagttgtat atgatgattt atccaatcag cttgctacac
    19381 cattcacttt gaatgcagat gaaatggaca aattgatcca aaagattgaa gacgcaggta
    19441 tcagtgtggt ggatgaaaat ggtgatccat caattcacag cttgaagagt gctgagaaaa
    19501 aagctgaaca agcaaaaaca gaagatcttt ctgcgccaac cggtgtaaaa atcaatgacc
    19561 cggttcgtat gtatctgaaa gaaattggcc gtgtctccct tttaactgcg gaagaagaag
    19621 tagcgttagc attgaaaatc gaagaaggcg accaagaagc gaaacagcgt ctggcagaag
    19681 caaaccttcg tttagtagta agtatcgcta aacgttatgt aggacgaggc atgcagttcc
    19741 ttgacttgat ccaagaagga aacatgggac tgatgaaggc agttgaaaaa tttgactacc
    19801 gcaaaggatt taagttttca acctatgcta catggtggat tcgtcaagcc atcactcgag
    19861 caatcgctga ccaagcacgt acgatccgta ttccagttca tatggttgaa acaattaata
    19921 aattgatacg tatccaacgt caattgctgc aagaccttgg aagagaacct acgccagaag
    19981 aaatcggtgc ggaaatggat cttccaacag aaaaagtccg agaaatcttg aaaatcgcgc
    20041 aagaacctgt ttcccttgaa acgccaatcg gtgaagaaga tgactctcac ttaggtgatt
    20101 tcatagaaga ccaggaagca acaagtccag cagaacacgc agcttatgag ttactaaaag
    20161 aacaattgga agatgtacta gatacattga ctgatcggga agagaatgtc cttcgcttac
    20221 gttttggttt agatgatggc cggacgagaa cattggaaga agtagggaaa gtatttggtg
    20281 ttactcggga acgtattcgt cagatcgagg ctaaagcatt aagaaaattg cgccatcctt
    20341 ctcgttcaaa acaattaaaa gatttcttag aataagcgtg gactattgac cagaagagaa
    20401 atcagtaaaa ctgtttggtt tattttaaaa gaaaaaaagc ttgagactac gcagtctcaa
    20461 gctttttttt ctataggaag gaaattcatt cattattttt aagtaaatcc taaaaataag
    20521 ctattggcgt gacgtataga atatgataga ttaaatggta gcgtaaacct agtaaaggag
    20581 ccgatcgcta agtgatgaaa aaatgtccaa attgcggatc gaaaaaaatg gagcagaata
    20641 ctatctgtcc agactgcggt catcaaataa ctgcagaaga tcagcaaata aaagaatcaa
    20701 ttgaagaaac aaatacagat gcagctatgc aggataaatc aactgaagat gcagtctttc
    20761 ctgatacaga attaaacgat ccaatcgaat ggtcggaatt gaaagattta cctctagaat
    20821 ctgtaatgga attattcgaa aaagcagaac cagaagacga agaaccacct gctcacgatc
    20881 aagtaactaa tatagagaaa aatgaacaga agaaagcaac tgttcatgag acttcggaag
    20941 aatcaggaaa gaatcaagaa gaaacgcttg aatcaaaaca aaaaagaaga gtagctgaac
    21001 tgaaagaaat cgtcgataat gaggaagaaa attcgatact atccgcttat atcaaagcac
    21061 atcgtgaaga tacaaaagaa gagcacgcga aagagctgct caaaatgatc agtgaaaaaa
    21121 ttgcacagga aaacaaagta gagccagaaa aggaacaaga aactgaaaaa gactctgata
    21181 gagaaaacgc cacaggtaaa gaaacagtaa aaataaaaga cgtaaaaacg gatatcacag
    21241 aaaaaactga agtatttact gaaaaacaat tagaaaagga aaaaacgaat aaactgccgg
    21301 aagcagagaa aaaagaaata ctcgaaaaca cagacaaaaa gtcaacagag cccgatgaaa
    21361 taaatagtga atccgatgct gaaaatcaat ctgtaagcaa agagggtact gatcttgaag
    21421 caaaaataga aaaagaagag aaccagccta agctgtttga agcagaagcg aaatcaataa
    21481 caatcgtgcc agatactgaa aaaacacatc aagaatcaaa cttagaaaaa acagatccag
    21541 ataaagcgca aaagaaaaaa tcaaaaaaag taccttatct cgttttagca gcagtacttc
    21601 tactcggtgg aggaagctgg gcttattatg accatcatca aaaagtagaa gccgaagtag
    21661 cagcagaagc aaaaagacag caacaaatgg aagaacttca tgagaaactt gctgcctttt
    21721 atacagatga tcaccatcaa tttattcgtg cagatatgat tcatcaagat ttatcaaaac
    21781 taaaaaatga tttatccagt cttaagaatg aaaaaggata ttcagagtta gaaacaactt
    21841 accaagatat ccaatctaag atccgagata tccaaaaagt gaatgagtgg ttcatagcac
    21901 ctgccatcgt cgatgatcat ctagcagatg acatcaagtt gaaagcagat aaagagatcc
    21961 aagcaatcga aacggataac acagattttg gaaaattgat tcaacaagca caaacagaag
    22021 ctaaaaatca atatgctgaa ttgcaaacag ctaaagaaaa aacagctata gtctttaaag
    22081 acggaaaagt agtggattca gcaacacgcg accaatacaa gacggcaaaa gaagcagtag
    22141 aaaaagtgaa aaatacagat ctatctgctc agttgaatga acaattgaaa caagtagata
    22201 agatgctcac tgataaagag aaaaaagaag aagaggaaaa gaaaaaagca gaagaagcga
    22261 aaaaagcagc tgaagaacaa gcaaaacaag ccgctcaagc agcagctgca gctcaagccg
    22321 ctcaacaagc tgcggtcgca caagcagcac agcaagaagc ggcatcctcg accccttcct
    22381 cttcaaactc agcgaatcag ccaatcaaat ctgccagagc aagtgatatt gccgatgcat
    22441 ctaatccagc atggacgtgg gcaccaggtg tccaaagcaa agtgattgct acatgtatcc
    22501 aacgtggata tatcgttgaa ggaggttaca gattagaaaa agccagaatc gaaaatgggg
    22561 aaggctatta taatctatat gccacttcaa caaaatctgc attgatgaaa ggcattggag
    22621 aaagtgcttt gccattttac atcgtaacca tcaactgtaa aactggatgg ttcggaggaa
    22681 atgggagcaa ttaattttag gcaaatgaaa gaggctgtga gcctgccgtt taacaccgtt
    22741 tattcgaaaa aggggagtat gacacaactg ttgtcatact ccttttttca cctttccaaa
    22801 ccaaggctca gctattcaag tagataacat tatgttatga tagcaaagaa atgtattaag
    22861 aaaacctttc actatagagg gagaaaaaaa tgaacgattt attagcaact atatttacag
    22921 gtattattat tgatgaaaat gaaaaaaact actttgtcca aaaaaacgga caaacctttg
    22981 gcttaagcaa atcagaaggt gaacacaaaa tcggtgaagc agttgaagga ttcggttacc
    23041 aaaatcaaaa acatgaaaat cggatcacta cacaaattcc taaaagccgt atcggtcatt
    23101 acgcttttgg aatcgttact gctacacgaa aagatttagg tgcgtttgtc gatatcggtc
    23161 ttccagataa agactttgtc gtatctcttg atgaattgcc gacaatgaca gagctatggc
    23221 ctaaaaaagg ggatcgcttg atgatttccc ttagagttga tgcaaaagac cgtatttggg
    23281 gcagtttagc agatgaaaaa atattcaaat ctttaagcaa atatggaaca gaagaaatga
    23341 aaaacaaaga tgtgatagga acagcatacc gcttgaaatt aacaggtacg tacgttttaa
    23401 cggaagattt ctatcttggg tttatccatc cttctgaacg atataatgaa cctcgtctag
    23461 gcgagcagat aaaaggtcga gtgattggtg tccgaccaga cggtgtactg aatctttcat
    23521 tgaagccaag agcatatgaa gctatatcag atgatgcaca aatgatttta accattttag
    23581 aacgttctgc agatcagcaa atcaacttta cagataaatc ggatccagaa gaaatcatga
    23641 aagcttttgg tatcagcaaa ggagcattta agcgcgcatt aggtaatctt ttgaaacaag
    23701 gattgattac acaagaaaac ggtgtgacaa aactagtcaa aaaatctaat tgagaatccc
    23761 ttgcatgttt ttcttaacta gcttataata attttaatta acatagtgtt atatcttttt
    23821 ttatttgtaa ttattataaa ttgttggggt tgagtaaatg gtcatgaatg ctatttcggc
    23881 gatgaaaaaa acaaaacagc agctacatga agcggggttt aagctgacac cgcaaagaga
    23941 agctactctg cttgtgcttt tggaaaatga gaaagagcat ttatctgcag aagaaatctt
    24001 ctttttagtg aaaaaaaaga gtccggaaat tggacttgct actgtttatc gaacccttga
    24061 aatcttgaca gaattgaaaa ttgtcgataa agtaagtttt aatgatggat tagcccgtta
    24121 tgatttgaga aaagaaggcg ccaaacattt ccatcatcat ttactatgct tgaactgcgg
    24181 cacgatcgag gaagtagaag aggatctttt acttgaagtg gagcaagtag tagaagaacg
    24241 ttatcatttt ttagtaaaag atcatcggct aacgttccat ggaatctgtc aggaatgcta
    24301 tgaaaaagaa a
//
LOCUS       DACDIG010000041        24095 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.041, whole genome shotgun sequence.
ACCESSION   DACDIG010000041 DACDIG010000000
VERSION     DACDIG010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24095
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.041"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            126..3653
                     /locus_tag="IV247_001697"
     CDS             126..3653
                     /locus_tag="IV247_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP8777769.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3650..7372
                     /gene="addA"
                     /locus_tag="IV247_001698"
     CDS             3650..7372
                     /gene="addA"
                     /locus_tag="IV247_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAP8777770.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7441..7782
                     /locus_tag="IV247_001699"
     CDS             7441..7782
                     /locus_tag="IV247_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8777771.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8091..9134
                     /gene="pheS"
                     /locus_tag="IV247_001700"
     CDS             8091..9134
                     /gene="pheS"
                     /locus_tag="IV247_001700"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAP8777772.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIVRKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9139..11559
                     /locus_tag="IV247_001701"
     CDS             9139..11559
                     /locus_tag="IV247_001701"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAP8777773.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11729..12739)
                     /gene="trpS"
                     /locus_tag="IV247_001702"
     CDS             complement(11729..12739)
                     /gene="trpS"
                     /locus_tag="IV247_001702"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAP8777774.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12993..13730
                     /locus_tag="IV247_001703"
     CDS             12993..13730
                     /locus_tag="IV247_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP8777775.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13744..14559
                     /locus_tag="IV247_001704"
     CDS             13744..14559
                     /locus_tag="IV247_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP8777776.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14572..15219
                     /locus_tag="IV247_001705"
     CDS             14572..15219
                     /locus_tag="IV247_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP8777777.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15235..15891
                     /locus_tag="IV247_001706"
     CDS             15235..15891
                     /locus_tag="IV247_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP8777778.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16053..16874
                     /gene="racE"
                     /locus_tag="IV247_001707"
     CDS             16053..16874
                     /gene="racE"
                     /locus_tag="IV247_001707"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAP8777779.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16877..18232
                     /gene="rph"
                     /locus_tag="IV247_001708"
     CDS             16877..18232
                     /gene="rph"
                     /locus_tag="IV247_001708"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAP8777780.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18233..18751
                     /locus_tag="IV247_001709"
     CDS             18233..18751
                     /locus_tag="IV247_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP8777781.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18858..19358
                     /locus_tag="IV247_001710"
     CDS             18858..19358
                     /locus_tag="IV247_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP8777782.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19638..20558)
                     /locus_tag="IV247_001711"
     CDS             complement(19638..20558)
                     /locus_tag="IV247_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAP8777783.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20723..21736
                     /locus_tag="IV247_001712"
     CDS             20723..21736
                     /locus_tag="IV247_001712"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAP8777784.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21846..22601)
                     /gene="fabI"
                     /locus_tag="IV247_001713"
     CDS             complement(21846..22601)
                     /gene="fabI"
                     /locus_tag="IV247_001713"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAP8777785.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22821..23261
                     /gene="fabZ"
                     /locus_tag="IV247_001714"
     CDS             22821..23261
                     /gene="fabZ"
                     /locus_tag="IV247_001714"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP8777786.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23376..24092)
                     /locus_tag="IV247_001715"
     CDS             complement(23376..24092)
                     /locus_tag="IV247_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8777787.1"
                     /translation="MLFAAGCSANNTTSSSSSTESSKSTSTAESTTASSVDLNALELP
                     QLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAKDGYYNGLTFHRVIKD
                     FMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARSQDPNSNGSQFFIVQN
                     TDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGMDVVDAIANIETDSSD
                     KPKEEVKIDKINILQEAKQK"
BASE COUNT         7972 a         4208 c         5102 g         6813 t
ORIGIN      
        1 gtatacttaa aaaagtagaa atatcagaac cttaaaagga tataatttga agaatataga
       61 acaaatgttc agtatttttt gatataataa aggagcaccg tatcatgtgt tgaaggagga
      121 aaagcatgtc tttacaattt attcttggaa acgggaaaaa agatcaccgt caagtcttga
      181 tcgacgaagc atccgcttgg ctcgaccagg atgcaagtaa ccaagtattt ttccttgttc
      241 ccaactacag caaattcgaa caggaacaag aaattttagc ggagatgcga agaagaagcg
      301 gaaaaaaaac tttcagcaca acaaacgttc aagtatttag tttttatcgt ctggcttggt
      361 atttgctgca acagacaact cttcttacag gaaatgaatt atcagaatca ggatctgcaa
      421 tgatcttgcg acagatttta gaaaaaaatg aggaagaatt aacaatcttt cgcggagaaa
      481 tcaataaaag agggtttatt cgtcaattac aagaactcta cagtgaattg cgtacaggaa
      541 atattcaacc agaagacctg atgcttttat ttggtgattc acctaatgca gaagaccagc
      601 aacttaaaat gaaagacttg aagctgattt tttcagctta tgacagcgaa ttgactcaac
      661 gtgcactaac aaatgaagat gcactatcta tactagcaaa ttatatgcag acacaagatt
      721 tcaacaacgt caaatttatt gtcagtggtt tttctcgaat caatggtcaa gagtatcaat
      781 tactgcaggc aatgatggaa aaggggcaat tggttgtcga tttattgctg gatcgtcctt
      841 atacagcgga tcttccagaa gtacttacgc tctttcatga gactggaaag ctttatttcc
      901 gtctttttca aagtgcaaga gaacggaaag tccctatatt agtggaccga tttgcaccag
      961 agcagactcg tccgacagag cttcaactgc tgcaaacaat gtgggaagag acatctagcc
     1021 aaaagaaagc ggcagtacac aaaatagggg gacaagagca tctccatttg tgggaagctg
     1081 aaaatccaac tgaagaagtc cgacaattag ctgttgaaat cagacgacta gtcagtgaag
     1141 aaaactaccg ttatcgtgat atccaagtgc tgacaaaaaa tatgtcttta tatggaaata
     1201 tccttcggcc gatctttcaa gaaatggcaa ttccttttta tatcgatgaa gaacaaatga
     1261 tggaaaatca tccgctgatc gaatggatca acagtctgtt cgctcttgac cgctattctt
     1321 atcggctaag tgatattatg cgttttttca aaactgaatt atttctgtcg acggaagcag
     1381 aaaccgaact tgatgaatgg aaacaagagc gagatgaatt caggaaaaaa ctggacatca
     1441 ctgaaaatgt agcacttgct tacaattatc aaggaactta ttggactcgt gaaaaagact
     1501 ggcagtttgt ggcgtatgat tttgaagcag aacagattga agacgtacag tatttggaag
     1561 aacagtcaaa tacgattaga cgattatttc agaaagctgt accagtattt ttgaaaaaaa
     1621 taaagaaaag caaaacagga caagaggccg caaatgtttt ttatcaattt ttgatccaaa
     1681 gtggcgtaca gcaacaatta ttgttttggc gtaatcagga agtagacaga ggaaacttag
     1741 aagcggcaag aaaccatgag cagacatggg gagcgctgat tgatctgttg gatgaatatg
     1801 tagtaattta tggtgaatcg gcttttgact ggtcactgtt ccaagaaatc attacaagtg
     1861 gtctagaaaa tctaagttat ggtaaaatcc ctaccgcaat cgaccaagtg cgaatcaatc
     1921 gtttggaatt agttcgacca aatcaagctg ccgtcacttt tgcattaggg ttgaatgatc
     1981 aagtgtttcc agaccgaaaa gagacaaaag gattgttgtc cagtgaagaa cgagaagtat
     2041 taaatcaggc cttgccagat ggacagtttt tattcgaccc atcaaaggaa agtatctctt
     2101 ttgagccttt ccaagcctat cttgtttttc tttccggaac agatcgatta tatcttagtt
     2161 atgcacaaag ctatgacaca gatggttcgc tgaagatgtc tccttatttg cgtagaatca
     2221 cagaatattt atctgttccc gtcgagagaa aagagcattt gacgatcgaa agcgacccta
     2281 attgttatgt agggacttac agaagcggaa tcaatacagt caataggatt tatcgattag
     2341 ctttagatga aaaaaaacct gtaccaaagc actggcaaaa gctcaaagaa ttagtactga
     2401 attcgtcatg gcgaaatttt gctttacaga tttttgaaag tcaggaacat ttaaatgttc
     2461 ctgtttcgat gccagaaaaa atggcggaag agctttatgg aaaagagatt tatacttcaa
     2521 tttcacgtat ggagaatttt tataattgtg aatacaaata cttcatgcaa tttggtttga
     2581 aattgaaaga acgaactgtt tatggtctaa ctccagcagc aactggtgat ttttatcatg
     2641 aatcattgga tcgtttcttc aagctactgt tttcaaacca attatcctta gtgaatatgt
     2701 ccgatgcgca gcgtagagac tttactgaaa aagtgctgca ggaagtattc ggagaacttc
     2761 ggtttgagat attggatagt tctgcaagaa tgaactatat ccgctaccag ttaggacaga
     2821 cgattcaaaa agtgacatgg gctttgcaaa aacagagcaa aaatacaggc atgcatcctc
     2881 ttcagacaga gattctattt ggtcagattg ctggagcaaa aggtatcaca ggtctggaat
     2941 taccgttgag taatggtgga aaactccatg ttcgagggaa aatcgatcga atcgatgtag
     3001 cttctgaaca agaagatact tggctaagcg tcgttgatta caaatctagc ggacgttcat
     3061 tcgatgtcac agaagcttat tatggcatgg ctatgcaatt attgacgtat ctggacgtag
     3121 cattgatgga tgcggtacgt ttgactggaa aggcggctgt taaaccagca ggatcatatt
     3181 atttacatgt tcataatcct gtattgacgg agacagatga tgtagagaag aatacattga
     3241 aaaaatttag ttatgatggc gtttttgtcg atgatcccgc tcttttagaa gtactagatc
     3301 attcactgga agcaaaagaa caatcactga tctatccagt gaaaaaaaat gcgaaagatg
     3361 aataccaaaa aggtagtttc agtaaagaca aatttttcac ggaagaagaa ctgcaactat
     3421 tcatggcgca taatcgggaa aatatgcggt tagctggaga tgcgattact tccggagaaa
     3481 tcaaactaaa tccagcttat aaagataaac agcggattgc ctgcgaattt tgtccattca
     3541 gaagcatctg tacgtttgat gtgatgctga aagaaaataa ttaccaccgt ctggagaaaa
     3601 tagataagga agaagcactg cgtagaatat tgaaaaggag tgaagaagag tgaatcaaat
     3661 tcctttaaaa ccgcaaaatg aacgattcac agatgatcag tggcaagcga tttttgatca
     3721 aggagataat ctattagtct ctgcgtctgc cggatcaggt aaaactacag tgcttgttcg
     3781 aagagtgatt gaaaaactaa aaatgggttt tgatatcgat gaattattga ttgtgacatt
     3841 tactgaagct gccgcaagag aaatgaaaga aagaattcaa gaggcattac aagaatccgt
     3901 caatagcgaa agtgaccctg tacgtagaca gcattttact aagcagttag ttttattgcc
     3961 cacagcgaat atctcaacct tacatgcttt ttgcctgaca gttattcgac gttattatta
     4021 cttaattgat attgatccag tctttcgcat gttaacagat gaaacagaaa cgatcttgat
     4081 gaaggaggat gtgtgggacg agctgagaga agcactttat gcagaaaatg atgaacgatt
     4141 tttccaatta acaatgaatt tttctaatga tcgttcagat gacggcttga cgaatctggt
     4201 gttttctctt tatgagtttg cccgtgccaa ccctgacccc caaaaatggc tagaacagtt
     4261 aagcgataat tatcgtttgc ctgaggggct agcgaaatct cgattatatc aaaaacaaat
     4321 ccgtccttta gttttggcag atatatacca atgtgtacag ttatatgaac agatgactca
     4381 gttagcacaa ggtgaaggat tagaaaaaat gcatgagcaa gtagctggag aacaacagca
     4441 gatcaagaat atctatgaag cgttctcgca agatcggtta gaagaagctt atgctggttt
     4501 ggaacaacta actttttcaa cttttaagag cagtcggaaa gcagaattga aagaaatttc
     4561 caatgaagtc aagggcatga gggataaagc aaaaaaactg attcagcaga taagtaaaag
     4621 ttattttcct gtttccccaa gccaaatgga agaattaacg gacaaagcac taccgttagt
     4681 cgaagaaatg acaaaagtca cgcaaagctt catggacggt ttcagtatgc gtaaacggga
     4741 aaaaggtgtg ctggatttca atgatctgga acatttagcg ttgcagattc tcacagaaaa
     4801 aacgaaagat gtatggctgc caagtgaagc ttcaaaacat tatcgaaaaa aattcaaaga
     4861 agtaatggta gatgaatatc aagacgtgaa ccaattacaa gaagccattt tatactggct
     4921 aagagaaccc gatgatacaa aaggaaatat gttcatggtt ggggatgtca aacaatccat
     4981 ctattctttc cgtttagcag atcctagttt atttatcggg aaatacgaga atttctcaaa
     5041 gaaagaaggc ggacgtcgaa tcgttttggc tgagaacttc cgctctagaa aagaagtcct
     5101 ttcttttacg aatcttattt ttgaacaact aatggatcca gcagtgggtc aaatcaatta
     5161 tgacgaagca gccaaactga tccaaggatt ttcagacttt ccagagaatg aacagtttga
     5221 gccggaaatc atgatctatg aaaaagaaca ggaagaatca gagattgaga ttccgacgga
     5281 tgatatactg gaagacaaaa cagaaggcga actttttatg acaggactaa aaattcgtca
     5341 attaattgat tcctccttta tgatttatga taaaaaatca aaaaaaagtc gtccgataga
     5401 gtacaaagac attgttctct tgacacctac aaagaagaac aacttaacga ttttagagat
     5461 tttcaaaaca ttggatattc cattagagat gaatgatgct caaaactatt ttcaagcgac
     5521 agaaattcgc acgatgatct cgctgctaca attaatcgat aatccctatc aagatattcc
     5581 gcttgcggct gtattgcgct caccgatcgt gggtctgatt gaaccagaat tagccagcat
     5641 tcgattagct aaccgtgcac atacttacta tgatgctgtg cttgcttatc aagcatcaaa
     5701 cgaagatgaa ttagcagcta agctggaaca ttttggcaag caactagagc attggcgaga
     5761 acttgctcgc cgttcttcga tcactgactt gttatgggat atctattatg agacgggata
     5821 tctagagtat gtggtcggtc tgccagcagg ggcacagaga caagccaatc tttatgcatt
     5881 ggttgatcgt gccaaagctt acgagcaaag ttctttcaga ggattatacc agttcgtgcg
     5941 tttcattgaa aaaatgcaag aaaaagataa agacttagca gaaccagtca tttcaataga
     6001 agataatgct gttcgcgtta tgacgatcca tgcaagtaaa ggcttggaat tcccagttgt
     6061 atttttgtta gatatgacaa aggagttcaa cttacaagac ttgcgcaatc ggtatgcttt
     6121 tgaagaaaaa cttggagcag gaattcgcta tatggatcct gaaacacgag tgttatatga
     6181 tacgttaccg tttcaagcca tcaaattggc aaagcaaaac aaattgttgt cggaagaaat
     6241 gcgtaaattg tatgtaggat tgactcgggc agaacaaaag cttttcattg tcggttccta
     6301 taaaaataaa gagcaaatga tccaaacgtg gtctgaagca gctgatcatg aggagttagt
     6361 ttttgatcct gcgctacgtt tgaagggcag aagcagcttg atgaattgga ttggttacgg
     6421 attgatccgt catcctgaaa tgcagaaata cttagaggaa gaaatttcga cttctcttct
     6481 tcaacattca aatgcacagt tttctatttc atggatgaat cagcaatcga tcattgaaca
     6541 aagacagttg cttgctgaaa aagaactggt taacttagat cagcagatga aagaagatga
     6601 aactttatta gcagattcgt tacaaaaacg tctggcatat gaatatcctt atcaagcttc
     6661 ttcacaaacc acaagttatc aatctgtatc tgaaatcaaa cgattattcg aagatccaga
     6721 tgatacacaa gaatcacgtt tgactctgga aagcagtcag aacaaagcag catcccgtca
     6781 atttcgttat acgcaagaac aactagcaga accaaaattt ttgcaaaaag atcggcaagt
     6841 gtcagcagct gctgttggaa ctgctacgca tgctttgtta caattactgc cattagagat
     6901 gccaactaca gagagtatcc atcaaaaatt acaggaattg gtaaggaaac ggttagtaga
     6961 cgaaaaagtc gcgaaaaaag tagatgtttc ttctattata tggttctttc aaacagaact
     7021 tggtcaacag ctgattgcca acaaagaaaa cgtaaaacga gaacaacctt tttccatgct
     7081 gcttcctgct gacgaagtat ttcaagacta tccaaatcaa gaagatgaac tattaatcca
     7141 cggaatcgtc gatggatacc tagaagaaaa agatcatctc aatatctatg attttaaaac
     7201 agattttatt ttacatccgg atgatccagc agaaatagat gcaatcgtcc aaaaatatca
     7261 aggacagcta agactttacc aacaagcaat gtcagaagct ttgaataagc cagtagaaaa
     7321 tgttttccta attttgcttc gagtgaaaca aattataaac attaacaaat aattataatt
     7381 gagcaaactt gacttatgtt ttgtaaggat tatgctatac tctgggaaga ggtgagtatg
     7441 atggagctaa cacagacaga attcgtttta tgccctaaat ttgaaaaaac attctctatt
     7501 ttaggaaaaa aatggaacgg attgattatc gatgtactac tcgaaaacgg tccgcaacgc
     7561 tttgttgagc ttgcggcaaa aatcccgatg gtcagtgatc gagtattagt cgaacgcttg
     7621 aaggaattag aaaaagaagg aatagtcact cgaaactttg cctgtagcga aaagagccgt
     7681 tcagaatatc tcttgactga aaaaggagca gacttgcaaa aagcgatgcg tcaaatacaa
     7741 gtttggagcg aaaaatggat cacagaggaa gaatgcggtt gattttgtaa aaaaaatctt
     7801 gtaaactata gtccatacga ataagtaaag aacgatgacg aaaaagagta gtttggctaa
     7861 accagttcag ggagatctgt cgcagactga aagcagatcc tgtgtttgaa aaatgaagtt
     7921 cactttccga gttcgtctga taaaatcaga ccggcaattg tcgttaacga agtaagagtg
     7981 cggtgtaagt catttatccg caaattagga tggtaacgcg aagacctcgt tccttaacag
     8041 gaatgaggtc tttttgtttt gtatttggaa tttaaaggag gaacaagcac atgtctctac
     8101 aagcacaatt agagagcttg aaagaagcaa cgctcgtcaa aatcacagcg gcacaagatt
     8161 taaatgaatt aaatcaaata cgtgttgaaa cgttaggaaa aaaaggaccg atcacagaag
     8221 tattacgtgg gatgaaagat ctaagtccag aagagcgacc aaaagtcgga agtttcgcaa
     8281 atgagatccg agatcttttg acagaagaga tcgtgcgaaa aaaacaagta ttagaagaag
     8341 aagcgttaaa tgcagctttg gcaaaagaaa caatagacgt gacactacct ggtagaaaag
     8401 taaaacatgg tacacgtcat atcgtgacac aaatcatgga agaaattgaa gatatttttg
     8461 tgggtatggg ctatcagatc gtagaaggat atgaagtaga atctgatcat tacaatttcg
     8521 aacggatgaa tctgccaaaa gatcatccag caagggacat gcaagatact ttctatattt
     8581 cagacgagat attgattcgg acacatactt caccagtcca agcacggaca atggaaaaac
     8641 atgatttctc taagggtgct ctacgaatga tctcgccagg gaaagtcttc cgcagagata
     8701 cagatgatgc gacccacagt catcagttcc atcaaattga agggctagtt gttgataaaa
     8761 acatcacaat gggcgacctt aaaggaacat tagaagtcgt aatgaaaaaa atgtttgggg
     8821 aagatcgtga aattcgtttg cgtccaagtt atttcccatt tacggaacca tcagtagaag
     8881 tagatgtcag ctgtttcaaa tgtggtggtg ccggttgtaa cgtatgtaaa tacactggat
     8941 ggatcgaaat tttaggagct ggcatggtac acccgaatgt gttgaagatg tcaggaatcg
     9001 atccagaaga atattcaggc tttgctttcg gccttggacc agaccgcgta gctatgttgc
     9061 ggtatggagt gaatgatatc cgtaatttct atcaaaatga cttacgtttc ttaagtcagt
     9121 tcaaggggga gtaagaaaat gttagtttca tataaatggc tcaatcgtta tgtagattta
     9181 tcgaatgtaa caccaaaaga attagcggat aagatgtctg tgacaggaat cgaagtagag
     9241 ggagtaacgg ttccagaaga aggcttgaaa aaaatcgttg tcggtgaagt caaggaatgc
     9301 gtaccacatc cagattctga tcacctttct atttgtcaag tcgatattgg cgaagacgaa
     9361 ctgtctcaaa tcgtttgtgg agcgcctaat gtgaaggttg gaatcaaagt gattgttgca
     9421 cttcctaact cacgtatcgc tggtaatgta aaaatcaaaa aaggaaaaat gcgtggccaa
     9481 gtatcaaatg gcatgatttg ctcattacaa gaaattggct attcagatag tgttataccg
     9541 aaagaatacg ctgaaggtat ctattatatg ccacaagaag ctgtaaatgg cgatccagtt
     9601 tttccttatt tggatatgga tgattcgatt attgaattat ctatcacacc gaatcgtgcg
     9661 gatgcattgt caatgcgtgg cgtagcttat gaagttgggg caatttatcg tcagactcct
     9721 acattcgatg acgaaaagct ggtagaatcc tcacgtcctg ctagcgataa aattcaagtg
     9781 gaagttgaag ataaagaagc agttcctgct tatcaaatca gaatcattga aggagtaaga
     9841 atccagccaa gtcctcaatg gctgcaaaat cttttgatga acgaaggcat tcgtccaatc
     9901 aacaatgtag tagatgtgac gaactatatt ttgcttttat tcggacaacc actacatgca
     9961 tttgactacg acaaattgaa atcaaaagag atctttgtcc gccgtgcaaa agaaaatgaa
    10021 acattagtaa cactagatgg agaatctcgg gaattgacaa ctgacaatct cgtcattact
    10081 aacggcaaag aaccagttgc tttagctggt gtcatgggag ggttagactc agagatcacg
    10141 aatgaaacaa caacagttgc actagaatct gctttatttg atccaatttc gattcgtcgt
    10201 acagcaaaac aatttaattt gcgtagtgaa tcctctgcac gttttgaaaa aggaatcaac
    10261 catgcgactg ttggagaagc cagtgaggtt gctgcagcaa tgatcgtttc tcttgcgggt
    10321 ggggaagttc tacaaggcgc ggtcaaaggc tcagagatca aagcagaaga cgtatcagtg
    10381 aagatttcac ttgaacgcat caatcaatat ttaggaacat ctcttgtcgt taaagaagta
    10441 aatgagattt ttgaagcact tggatttagc tatgaagaaa aggagtatgt ttattccgta
    10501 gtagtaccac caagacgctg ggatatccaa atcgaagcag atattatcga agaagttgct
    10561 cgtatttatg gatacgacca gttgccatct acacttccaa gtggagaaac ggttgctgga
    10621 agcttgtcaa aagaacaaga aactactcgt aaaattcgta caatctttga aggaagcgga
    10681 ttgagcgaag caatcagtta tgcattgaca acagaagaaa aatcaagaca gtttgttata
    10741 aatgaaagta atattacacg tttggactgg ccaatgagcg aagaacgttc tgtattacga
    10801 atgaatttga tcagcggact attagatgat atcagttata acactgcccg aaaaaataca
    10861 gagattgctt tatatgaaat tggtcgggtg ttcttccaag aaaatgatcc acgtacacat
    10921 ctgccgcaag aagtaaaaca tgcagcaatc gctatttctg gtatttggga agaaaaagat
    10981 tggcagacga aagctcaaaa tgtagacttt ttcaccatca agggtctttt agaaaactta
    11041 tttgaacaac taggcatcac tgaagatgtc cattatcaaa cagtgacaga aatgaaggaa
    11101 atgcatcctg gaagaactgc tgcgatttac cttggcgaaa aatttattgg ttttgtcggt
    11161 caagtgcatc caacgattac aaaaagttat gatattccag aaacatatgt tgctgagttt
    11221 gatttatctg ctatagtgga agctgctcaa aaagggatcg ttttcgaagc agtgacaaaa
    11281 ttcccagcag tcagccggga catcgctttg cttgtaaaag aaactgtgac aaatcaggaa
    11341 ttaacagaag ttatccgttc tgctgccgga agatttttaa cagatatcca attatttgat
    11401 gtctatcaag gagaaaacat tgaaaaagga cataaatcca tggcgtatag cctgacattc
    11461 gtcaacccag aagcaacatt gacagatgaa gaaatcaata aagcaatgga aaaagtcgaa
    11521 aaagcgttgg tagaaaacct agaggcaagt attcgctaag aaggaaaata tagtaagtaa
    11581 cgatcaagta gcttttgaag accatacatt tgaaaaaaag atgtgacaca acttcgtcac
    11641 atctcttttt ttgattttaa aaaaggaata gcacagagac gagataaaaa aacaggcctt
    11701 tacagcctgc ttttttagag taaatgaatt aatggaagta attgattccc attgctgatt
    11761 ttacttcaga aagtgtttgt gcagctactt tagcagcttc ttcactacct ttacgtaaga
    11821 tttccataac agctgaagga tctttttcca attcttctcg gcgcgcacgg atcggtgcaa
    11881 attcagcttc caatacatca atgaggtatc gtttgatctt tacatcacca agtccgccgc
    11941 ggcggtaatg agctttcatt tcttcaattg cttctttatc tgttccgaat acatccaagt
    12001 aggtgaagac catattccct tctacttgtc caggatcttg tacatggatg tggtttggat
    12061 cagtatacat actcatgact tttttctgta agacatcggc aggatcggag atataaatac
    12121 cgtttcctaa cgatttactc attttgccat ttccatcgat tccaggtaat ctacccattc
    12181 cttttggtgg gaatacgcct tttggttcca ccaagacttc accatatgta tgattgaagc
    12241 tttgtacgat ttcctgtgtc tgctctagca ttggtttttg atcctcacca acaggtacaa
    12301 gattcgcttt gaatgcagtg atatctgctg cttgagaaac aggatagata aagaatcctg
    12361 taggcacgct ttcaccgaat tttttttgtt cgatttctgt tttgacagtc ggattgcgcc
    12421 gaacgcgtcc tacgctgact aagttcaaat aatacatggt taattcagct aattccggaa
    12481 tttgtgattg gataaataaa gtagattttg caggatctaa tcccacggct aaatagtcta
    12541 aagcaacttg caagacattt gatgaaacct tctcaggatt tttagcatta tctgtcagtg
    12601 cttgcatgtc agcaatcatg acaaacagtt ggttgtcttc atctgcctgc atagccactc
    12661 gggtttttag agaaccgacg taatgcccta aatgcagctt cccagttggt cgatctcccg
    12721 ttaaaataat atttttcaaa aaagacacct ctttacgatt tgtaagtctg agcacttaat
    12781 atcttaccgt ttttcctgaa aagaaacaac agttcctaag gaaaaaacaa ggaaagagga
    12841 taaaataact aatagattag aaatggaaga aatatttatg ggagaataaa cgttctaaga
    12901 aactttacaa attactaaac tatcagatat aatgtatttt attatacatc tttaaaagaa
    12961 atcttactaa ataataatgg gagtgtaatt ctatggcaat gattaaattt gatcacgtgg
    13021 ataaatatta tggcaagttc cacgcattaa aaaatatcaa tctcgaattc gagaaaggag
    13081 aagttgtcgt tgtcattggt ccttctggtt caggaaagag cacgatgctt cgctgtatca
    13141 atggattaga gacgatctct tcaggtaagc tgctgattaa tgatactgac ttgcatgata
    13201 aaaagacaaa actgacagaa gtccgaaaaa acatcggcat ggttttccaa catttcaatt
    13261 tgtacccaaa taaaactgtt ctggaaaaca tcaaattagc accaatcaaa gtgttgaagc
    13321 aagaccaggc aactgcagtc aaaaacgcgg agaaattttt gaaaactgtc aatatgttag
    13381 ataagaaaga ttcttatcct tccatgttat ccgggggaca acagcaacgg gtggcaatcg
    13441 ccagaggttt agcgatgaat cctgagatgc tgctgtttga tgaacctact tctgctttgg
    13501 atccggaaat gattggtgat gtattagacg ttatgaaaaa attggctcat gacggtatgt
    13561 caatgattgt cgtgacacat gaaatgggct ttgccaaaga agtggcggat cgtgttatct
    13621 tcatggctga tggagaagta ttggaagaca gcagaaatgt gagagacttt ttcgaaaatc
    13681 caaatgaagt ccgagcacag caatttatca gtaaagttat caatcattga taggaggaaa
    13741 aagatgcgca ggttaaaaaa aatacgcctg attttccttt taggcattgc ttttcttgca
    13801 ctagccggat gtaaaggaaa tgggttggca aatgaagata ttttagagcg aagcaaatct
    13861 accaatgaaa tcatctgggg tgtcaagtat gatactcgat tatttggaat gatggatatt
    13921 gagagcagaa ctgtacaagg atttgatgta gatattgcaa aagcaatcac taaaaagatc
    13981 cttggtgaca atggaaaaac agaatttgtt gaagtcactt ctaaaactcg gattccatta
    14041 ttgaaaaacg gaaatatcga tgcaattatt gcaacgatga cgatcacaga tgaacgaaaa
    14101 aaacaggttg acttttcaga tgtctatttc gatgctggcc aagctttgtt ggtaaagaaa
    14161 ggaagccaaa tcaaaagtgt agatgatttg aatgcttcta caacggttct agcagtcaaa
    14221 ggttctacat cggctgcgaa tatccgacag catgcaccag atgcaaaaat tttagaatta
    14281 gaaaactatg cagaagcatt tactgcttta caatctggtc agggagacgc gatgactacg
    14341 gataatgcga ttttgttagg gattgcagat gaaaatccag agtatgagtt agttggtgga
    14401 acgtttacta atgagcctta cgggattgcg atcaacaaag ggcaagaaaa cttcttgaaa
    14461 gctgttaacc aagcgctaga ggaaatgcat gcagatggca catacgataa aatctatcaa
    14521 aaatggtttc caaatgaaac tgaaggaaaa gtagagtaag ggggagaggc aatgagcgaa
    14581 ttgatacaaa cttatggacc aatctttgtc gacgggttca agatcacatt gttttcgagt
    14641 gtcttagcac ttgtgtttag tttgattatc ggaacgttaa tggcaatctt ccaattatca
    14701 aagaataaag tagtgagcgc cttagcaaag gcatatgtag aattttttag aaacattcca
    14761 ttattaatca tcgtgatgtt tttttatgtt gtcgtaccga tttattggtt taatctagat
    14821 ggattccaag caggtacgat tggattaacg atctatacat ctgcttttat tgctgaaaca
    14881 gtacgttcag ggattcaaac cgtaccaaaa ggtcaaacag aagcaggact atcttctggt
    14941 tttacctatt cagaaatcat gcgttatatt gttttaccgc aagcattcaa aatcgttatc
    15001 ccgccattag gcaaccaatt tatcaacctc gtaaaaaact catctatttt agcaatcgta
    15061 gctggtttag atttgatgta tcaaggagac ttgatcgcta gtgaaacatt caataccttc
    15121 gatacgtata tcatcgttgg gttattttac ttagttatca cgctaccgtt atcttactta
    15181 atggtttatc ttgagaaaaa atgggcggtt cgttcataga aggaggggaa atacatggat
    15241 tttagcggcg ctttttcatg gatgaatatc cgttttttat tggaaggatt aaaggttact
    15301 gttgaagttt cattgttttc gatcattttt agtttcttga tcggtggtct gacaggtgtg
    15361 attcgttttg cgaatatccc tttcttttcc aaaatccttg gtgtagtgat tgatattata
    15421 cggaatcttc cattattatt gatcattttc ttcacttact ttgccttacc tcaaatcggc
    15481 attcagatga acatcttctg gtccgctgtc gcagcattaa cgatttttga gtcagctatg
    15541 ttgtcggaaa ttttccgagc tggactcaat gctgttccaa aaggacagat ggaagcagga
    15601 ttatcaacgg gactgactta tgtagaatcg atgcgaacga tcgtgttacc gcaagcattt
    15661 aaatcaatga tcccggcaat tgtgagccaa ttgatttcac tgatcaaaga tacttcatta
    15721 gcagtaatca tttctttacc tgaactttct catcaggcac gtattattta tggacagaac
    15781 accaattatg tgcttccgat gttcatcatc atgacactta tgtacttcgt ggtatgttac
    15841 gcgctttctc tgctttccgg ttacttggaa aagagacggt acagttatta atagatgatt
    15901 cttttagcgg ttgtggcaaa ctaaagtcac aaccgctttt cctctttcca ttcgaaaaaa
    15961 agagatttcg attgttttaa gcaagtaaga gcacttgaaa attcacgcta aaaagtacac
    16021 aatagggagg atagaattta ttgaaaaaaa ggatgatacg attgacagat aatcgcccta
    16081 tcggatttat tgattcaggt gtcggcggct tgactgtagt aaaagaagcc ctgaaacaat
    16141 taccgaatga aaatatttta tttgtaggag acacagcacg ctgcccatat ggccctagac
    16201 ccgcggaaca ggtaatccag tatacttggg aaatgacgga ttatctggtg gagcaaggaa
    16261 tcaagatgct ggtgatcgcc tgcaataccg caactgcggt ggctttagaa gaaatcaaag
    16321 ctgctctttc tattccagtc atcggtgtga tccttcccgg tactagagcg gcagtaaaaa
    16381 aaacacaaaa taaacaagtt ggcattatcg gtacgattgg tacggtaaaa agtcaagctt
    16441 atgaaaaagc actgaaagag aaagtaccag aattgactgt gacaagtctt gcttgtccca
    16501 aatttgtttc agttgtcgaa agtaatgaat accattcatc ggtggcgaaa aaaattgtgg
    16561 cagaaacatt agctccttta accactaaaa aaatcgatac attgattttg ggatgcaccc
    16621 attatccatt attacgcccc atcattcaaa atgtaatggg agaaaatgtt caactgatcg
    16681 attctggagc agaaacagta ggtgaagtat ctatgctgtt agattatttc aatttgagca
    16741 attcaccgca aaatggtcgg acattatgcc agttttatac aactggctct gccaaacttt
    16801 tcgaggaaat agctgaagac tggcttggaa tcggacactt aaatgtagaa catatcgaat
    16861 tgggaggaaa ataaggatgc gtcatgacgg gagaaaagcc caagagcttc gcaaactgga
    16921 gcttcaaaca aatgttttca aatatccaga aggatcggta gtgatccgtt ttggtgatac
    16981 aacagttatt tgttctgcaa caatagaaaa ttctgttccc ccttttttga gagaaacagg
    17041 aacaggatgg gtgacagcag aatatagtat gctgcctcga gcaacgagta cgagaaatcg
    17101 tagagaaagc agcaaaggga aactttctgg tagaacaatg gaaatccagc gtcttattgg
    17161 tcgttccttg cgtgcagtag tagacttgga aaaattagga gaacgtagta tcatcgtaga
    17221 ctgcgatgtg atccaagcgg atggcggtac tagaacagca agtatcactg gggcatttgt
    17281 ggcattgaaa ttagccattg aaaaactttt aagagaaaaa gaattgtcag aagatccaat
    17341 aaaagaacat cttgctgcag ttagcgtagg aatcttacca gatgggactt gcgtcacaga
    17401 tttggattac caagaagatt ccgcagcact tgtagacatg aatcttgtaa tgacagaatc
    17461 tggaaaattc gtggagattc aaggaactgg agaagaagca acgtttgatg gtgaacagtt
    17521 gaatgaaatg ctcttttttg gaaaaaacgc gattgaagat ttgatcaaag agcaaaaaca
    17581 tgctttgcta acagagtttg ctcaaaatga tgagcggata gaagaaacaa aaacaatcat
    17641 cattgcgaca agaaatccag gaaaagcaga agagtttcga aatatgttca aagaagcggg
    17701 atatcatgta aaaacattac ttgactatcc tgaattgcca gatgtggaag aaactggcag
    17761 cacatttgaa gaaaatgccc gattaaaagc agaaacgatt gcacaattgc ttgatcagcc
    17821 agtcttagca gatgattcag gtttgaaagt ggatgcttta ggcggtatgc caggaatcta
    17881 ttctgcacgt tttgcagggg aacaaaaaag tgatgcgggg aataacgcca aattattata
    17941 tgaattgaca gatgtgccgg atgaaaaaag aacagcacaa ttccattgta cgttagtatt
    18001 cgcggcaccc aaaaaagaca gccttgtagt agaagcggaa tggcccggtc gagtagcccg
    18061 tataccatct ggcgaaaatg gctttgggta tgatccttta tttatcccag aaggtaagaa
    18121 acaaacagca gcagaattat cgagcgaaga aaaaaacaag atcagtcatc gggctcaagc
    18181 aatgaaaaaa ttgagtgccg agtggaaaca atggctggaa ggagagagat agatgcggta
    18241 tttagtagta agtgataatc atggagacag agatatcctc gttgacttag tagatcgtta
    18301 tcgttctgaa gtggatatct ttttccattg tggtgattcg gaattagaag cttctgaccc
    18361 attatgggat acattccaag tggttcaagg taattgtgat tatggaccag gattcgaaca
    18421 gaaaaaagtg atccaaactg gtcaggatac agtattcatg actcacggtc atttatcgaa
    18481 tgtccgattt ggtttgacac agttagcaat cgaagctcga gctgcaactg ccgatatggt
    18541 gttttttggt catacccatc aaataggctg tgagatggat cagaaaatcc tgtttttgaa
    18601 tcctggtagc atttctcagc ctagaggccc gatccaaatc ccatcatatg ctcttatcga
    18661 aagtttagat gaccaaattt ctgttcaata ctataaccgt gcccatcagg cagttgaaga
    18721 gttggccttt tctttttcga gagagcgctg aataaaaaaa ctctaatttt taataaaaat
    18781 aagctttatt ttatgaattt tgcatccggt ttcggtaaaa tagtagtgtt gaatgaaata
    18841 gaaatggagg ccatcagatg attggaccta tagtaagaga aatgctgctg gaaaatcagg
    18901 aaacgttttt gattcctgca gaaaatgtgg caaatgttat gtatcatcat ccattgtccc
    18961 atggtttact tgtgttatcg aaggtcggtt attccaaaat tcctgtatta ggaaaagatg
    19021 atcactttgt aggactagta agtttatcag atgtggtcaa taaaatgatt gacttgcaaa
    19081 caatcagcat ggacccgtta gaaggactaa cggttgctga tgtgacagaa acagatgttc
    19141 cgacaattga tgaaaattgg gaactagaag aagtactcca tttgttagtc gattcgcctt
    19201 ttttaccggt tgtcacagaa gatcggattt tcaaaggaat cattacaaga aaagagctgc
    19261 ttaaagccgt aaacttcatg gtgcatgaat tagaacgtcg taatatattg tctccgaaaa
    19321 tcgaagaatt aaaagaacaa atcgatatcg tgagctaaag aacgagaaac taaaagataa
    19381 tcaaaaaata agtagaaaaa acagcgtacc agttttgact ttatcttgaa aaagaggctg
    19441 ggacaaaagc gtttaactcc aagaaataag aaggaattca cgaaaattgc ttttcaaatt
    19501 tttgtgaatt tcagcttatt tccgaaggag ttgcttctgc ttccgccgtt tatacgtgtt
    19561 tagagcgtgg gacaaaagtg ttctttactt ttgtcccacg ctctttttag tgagattcgt
    19621 tgcatcgttt gtccaaatta ttttccgaca gcaattggac tgttcggaat cgtgaaacca
    19681 gaagctgtca attctttgac ataggagctc aagaactctt cttttaaggc atattgtttt
    19741 ccgttcaatg cgtagcagac cgtacgaatc gcaaaattgc tgtttccaag atccactaat
    19801 ccaaagatag aaggttcagt ttggagctct tcttggtatt tttcagctaa tgtacgattt
    19861 actcgatcaa tgatctcata gattttgtca tatccttctt caggaacaat ccgaatatct
    19921 aaaagtactt gcatatttgc acgagagaga ttgctgatcg tggtgatgtt tcgattcggg
    19981 atgaaatgga cagtaccatc cgttgctttc agctgaaggg tacggatacc aacagatacg
    20041 acggttcctt caatcgttaa gtttgctagc ttgatgtagt ccccaacatc catctgttgt
    20101 tccatgataa taaaaaatcc ggtaataaca tcactcatga acccttgggc accaagaccg
    20161 attgccacac ctgcaattcc ggcaccagcc aaaagtgatc cgatcggcac accgatagca
    20221 gataaaattg catagataaa aaagaaacca agggtataat gaaaaatagt atttaaaaga
    20281 ctatgtaaag tctttaagcg gctctcgttt agaatggctt ttttagaata ctgattaaaa
    20341 gacttatcaa tcagatattt tcccacacgg tgaagaatcc aaaaaaggac gatcaaaaaa
    20401 atcagatata aacttttttg gataaaggca gccaaaatgg cgtcccaatc aatcccgtcc
    20461 caaaaccgct gaaacgcatt tactttgctg acagcttgat ctgtcaatgt ctcgccagaa
    20521 gagtctgtcg taccggcagt tgctaataat gaaaacataa aatcctcttt tctaaaattt
    20581 gattctttct gtattgtacc aagaagtttt caaaaaagga aatagcaaaa ataactataa
    20641 tgaaggaaaa aagctgaaaa taaaatgaca aataatatgg ataatgataa aatgtggaga
    20701 aaataacgaa ggtggtaagt atatgcaaat cgattggaac aatctaggtt tttcttatat
    20761 caaaactcct tggcgtttta ttgccaaatg gaaagacgga caatgggaag aaggagaatt
    20821 gacagaagat aattacctga cgattcatga aggctctcca gcgcttcatt atggtcagca
    20881 atgttttgaa ggtctaaaag cttatcgtcg aaaagacggc aaggtcaatc tctttcgacc
    20941 tgaacaaaat agtcgacgga tgaatcaaag tgctagacga ttgctgatgc caatggtccc
    21001 tgaaaagatg ttcgttgatg ctgtcaagca agtggtaaaa gcaaatgaag attttgtgcc
    21061 gccctatgga acaggcgcta cattatactt gcgcccttta ctgattgggg tcggtgaaaa
    21121 cattggtgtc catccagctc cagaatattt atttgtcgtc ttctgtacgc cagtaggagc
    21181 gtatttcaaa ggtggactaa aaccaacgaa ttttattgta tctgattacg atcgtgctgc
    21241 tcctaagggg actggtgcag caaaagtcgg cggaaattac gcagcgagtt tacttccagg
    21301 aacagaagca aaagaactgg attactcaga ttgtgtttat cttgatccag ccacacatac
    21361 gaaaatcgaa gaagtaggtg cagcaaattt ctttggaatt acaaaagacg gaaaattcat
    21421 cactccgcaa tcgccctcta tcttaccaag tatcacgaaa tattccttgt tgcagttagc
    21481 tgaagaacga ctaggactgc aagcaattga aggagatatt ttcattgatg atctagatca
    21541 atttgcagaa gctggtgcat gtggtacagc ggctgttatt tctccaatcg gtgggattta
    21601 tcacaataac aatcttcatg tattttatag tgaaacggaa gtaggaccag taacaaaacg
    21661 gttgtatgat gaattgacag gtattcaatt tggagacata gaagcaccag aaggctggat
    21721 tgaagtcgtg gaataatttc aaagtaatga gttgaaaaaa acgtaaaaag aaaaaatcag
    21781 gaagagactt taaaaagttt tttcctgatt tttatatgtt ttactttaaa gaatgcaaca
    21841 aaagtttatg aaagatggac acctttgtca acgtagataa tatcaccgat gattccgctg
    21901 gaaagtgggc tgattaagaa agcacaagca ttaccgactt cttcgatcgt cacaccttgc
    21961 tgatcaggcg tacgagattc agataactgg atcagttttt gatagtcttt aacacctgtg
    22021 acagctaatg ttttgattgc cccagctgag attccattga cccgaattcc tttaggcgag
    22081 aactcagctg ccaaataacg gatagcagct tcaagagaag ctttcgcaat acccatcatg
    22141 ttataattcg ggattgcgcg ttctgaacct agataagtca tgctaacgat cccgctgttt
    22201 ggtgcaagaa acggttgtgc gtattttgat acagcaatta aagagtagct gctgatatct
    22261 tgagctaatt gatagccgtc tctggaaata tcagacacat ttccggacaa ttcttctttg
    22321 ttggcgtaag caactgcatg gacaaggccg tggatctccc cagcatattc accgatggca
    22381 ttcattgctt tttcgatgct ttcgtcgcta gaaacatcac attctacagt gaatgcttct
    22441 tcgccgacca atttgactaa tgattttttc attcgttcat tttggtaagt atagataatt
    22501 tctgcccctt gtttcaacat tgcttcagca caaccccaag caatgctttt tttgttagcg
    22561 acgcccataa tgacgatttt ttttccttct agaaatgaca taatgactct cctttgtttc
    22621 tgataaaaat ttgatagtcg taaaaaaata gagaataata gctaaaataa cttcttgatt
    22681 tcttaattat tagtcaaaat gttttgattg tcaaactatt ttttcttaaa ttaaaaaaag
    22741 attgctaaat atttaggaat atgatacttt gatagtcaaa gtaatgaaaa tggcaattgc
    22801 ctgaaagaag gagtttaatt atgtcttacg tattatctgc tactgaagta atggaattga
    22861 ttcctaatcg ttatcctatt tgttatattg attatgtaga tactttagaa ccaggaaaaa
    22921 agatcgttgc gacaaaaaat gtaacaatca atgaagattt tttccaaggt cactttcctg
    22981 acaatccagt gatgccgggt gcgttgattc tagaaacact ggcacaagca ggttcgatac
    23041 ttatcctaaa atcagaagag tttctaggaa aaagagctta tatcggtggg atcaataagg
    23101 ccaaattccg tcaaaaagtt gttccaggag atgtgctgaa atgtcatttt gaaatcacca
    23161 aaatgaaggg atcagtcggt acagcgttat ctgcagcata tgtagatgat aaaaaagtct
    23221 gtgagtgcga gttcacgttc atcgtcaacc aaaatgcttg actataaaac ctgatatagc
    23281 aaaacaaaag gaagcttact tgctttttat gtaaaaagaa gacgcctgaa tcctagcata
    23341 cgttgtcatc tatgcaggat tcagacgtta tttttttatt tttgttttgc ttcttgcagt
    23401 atattgattt tatcgatttt tacttcttct tttggtttgt ctgaactatc tgtttcgata
    23461 tttgcaatag catccacgac atccattcct tcaatcactt ggccgaagac agtataatgg
    23521 ccatctagtg aaggataacc gccgttttta taagcgtcga tgattgcttg aggatagtcg
    23581 tcttttaaaa gaccatcatg catatcatct gtattttgaa cgataaagaa ctggctgccg
    23641 ttactgttag gatcttgtga gcgagccata gataacgcac ctcttatatt ataaaggtgg
    23701 ttgttgatct ctgtttcaaa tccttctccc caaatgcttt cccctccagt gccatcgcct
    23761 ttaggatcgc caccttggat catgaaatct ttgatgacgc gatggaaagt cagtccgttg
    23821 tagtaaccgt cttttgcatg tgtcataaag ttttcaaccg cttttggtgc gtgttcggga
    23881 aataatttga ttttgattgt gcctttattc gtcaccattt cgaccagatc ttcattttcg
    23941 gcgacatctt tcgtcaattg tggtaattcc aatgcgttca aatcgacgct actggcagtc
    24001 gtgctttccg cagtagatgt actttttgaa ctttctgtag acgaactaga agaagtcgtg
    24061 ttgtttgcag aacaaccagc agcaaacagc aatgc
//
LOCUS       DACDIG010000042        23575 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.042, whole genome shotgun sequence.
ACCESSION   DACDIG010000042 DACDIG010000000
VERSION     DACDIG010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.042"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(116..568)
                     /locus_tag="IV247_001716"
     CDS             complement(116..568)
                     /locus_tag="IV247_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777788.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKCNWKRMYFYIDGDDALTFGKTTFSSFNFQFLLKNNFYGLLK"
     gene            complement(575..1123)
                     /locus_tag="IV247_001717"
     CDS             complement(575..1123)
                     /locus_tag="IV247_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP8777789.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            complement(1168..1500)
                     /locus_tag="IV247_001718"
     CDS             complement(1168..1500)
                     /locus_tag="IV247_001718"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777790.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(1514..1744)
                     /locus_tag="IV247_001719"
     CDS             complement(1514..1744)
                     /locus_tag="IV247_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777791.1"
                     /translation="MCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQKGYPYGPTN
                     LKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(1966..2508)
                     /locus_tag="IV247_001720"
     CDS             complement(1966..2508)
                     /locus_tag="IV247_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777792.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            complement(2529..3620)
                     /locus_tag="IV247_001721"
     CDS             complement(2529..3620)
                     /locus_tag="IV247_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP8777793.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(3628..5838)
                     /locus_tag="IV247_001722"
     CDS             complement(3628..5838)
                     /locus_tag="IV247_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777794.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(5854..8382)
                     /locus_tag="IV247_001723"
     CDS             complement(5854..8382)
                     /locus_tag="IV247_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP8777795.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            complement(8434..8844)
                     /locus_tag="IV247_001724"
     CDS             complement(8434..8844)
                     /locus_tag="IV247_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777796.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            complement(8857..9075)
                     /locus_tag="IV247_001725"
     CDS             complement(8857..9075)
                     /locus_tag="IV247_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777797.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(9072..10064)
                     /locus_tag="IV247_001726"
     CDS             complement(9072..10064)
                     /locus_tag="IV247_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8777798.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            complement(10143..11054)
                     /locus_tag="IV247_001727"
     CDS             complement(10143..11054)
                     /locus_tag="IV247_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8777799.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(11073..11417)
                     /locus_tag="IV247_001728"
     CDS             complement(11073..11417)
                     /locus_tag="IV247_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777800.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(11437..11993)
                     /locus_tag="IV247_001729"
                     /pseudo
     CDS             complement(11437..11993)
                     /locus_tag="IV247_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(12074..12574)
                     /locus_tag="IV247_001730"
     CDS             complement(12074..12574)
                     /locus_tag="IV247_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777801.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            complement(12575..13120)
                     /locus_tag="IV247_001731"
     CDS             complement(12575..13120)
                     /locus_tag="IV247_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777802.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(13117..14373)
                     /locus_tag="IV247_001732"
     CDS             complement(13117..14373)
                     /locus_tag="IV247_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP8777803.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            complement(14734..15045)
                     /locus_tag="IV247_001733"
     CDS             complement(14734..15045)
                     /locus_tag="IV247_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777804.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            complement(15068..16378)
                     /locus_tag="IV247_001734"
     CDS             complement(15068..16378)
                     /locus_tag="IV247_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAP8777805.1"
                     /translation="MYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLV
                     RMLIDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGH
                     LLSEVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPN
                     MLIAGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEE
                     MIICLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDME
                     ARNRVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYL
                     MTFGEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYD
                     FTVKEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            complement(16992..17444)
                     /locus_tag="IV247_001735"
     CDS             complement(16992..17444)
                     /locus_tag="IV247_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777806.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            complement(17446..17811)
                     /locus_tag="IV247_001736"
     CDS             complement(17446..17811)
                     /locus_tag="IV247_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777807.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            complement(17910..22193)
                     /locus_tag="IV247_001737"
     CDS             complement(17910..22193)
                     /locus_tag="IV247_001737"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HAP8777808.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            complement(22340..22579)
                     /locus_tag="IV247_001738"
     CDS             complement(22340..22579)
                     /locus_tag="IV247_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777809.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            complement(22669..22941)
                     /locus_tag="IV247_001739"
     CDS             complement(22669..22941)
                     /locus_tag="IV247_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777810.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            complement(23024..23275)
                     /locus_tag="IV247_001740"
     CDS             complement(23024..23275)
                     /locus_tag="IV247_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777811.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
BASE COUNT         6975 a         5090 c         3436 g         8074 t
ORIGIN      
        1 aacaatcatc tatactcata taatcactcc actaattaat ctccccaaaa agacacagca
       61 ttaagagata taccaacaca acagtgcaaa taactctaca tcttccttcg caaccttatt
      121 tcaataaacc ataaaaatta ttttttaata gaaactgaaa attaaaacta gaaaaagtag
      181 ttttaccgaa tgtaagagca tcatcaccat caatatagaa atacattctt ttccagttac
      241 atttaatttt taaacaaaat tctccccaaa aaggaacttt ttctagtgaa aacttggtaa
      301 tctctttgta attaattctt gttaaagaat aattaaacac ttcattttta cttgttattt
      361 tttcattgac tataactagt tcatctggta aaaaaattaa ccaacaaaca tcgacaaaca
      421 gtaaatgata taatccattt ccacctccaa gcatattcaa tactggtgaa catgccttag
      481 aaacacgaat atagttatgg tttaaataga tattttcttt aaacattgct tgtttaaaag
      541 ccatatgctc ttgttctaaa cttttcataa acttttactc taatgcacag ctagcagcag
      601 ctgctgcaat tcctgctaca atatttacct tagctaactc tttagctgca gcttttccta
      661 attgtttttc tgcaattttt aataataaat ccgcagcttt ttcaaaatta aaagctttta
      721 atgcttcaat aaaatctccc tgcgtaccta tagcccaagc cactcctcct ccaggaagag
      781 aattaatggc tacacatcca gcatatcgtt tccaatcaac tttctccatt ctaggaacaa
      841 taatatgttc ataaaagact cttgctgctc ttccttgttc gttatcttga ctgattcgtt
      901 cacgaatagc accaaaatcc tttatatggt atacaccatg ttcgtctaaa aaaccaactg
      961 tatcaaaata aaaagttaat tcatctacta atttattctt tatttctaaa tcaactgtat
     1021 tcactttttc tgtggcatac acacttgttc cagtactaac aaaagtactt aaacctaata
     1081 agaaaacacc taaaacacca aatattttaa ttattctttt cataatttct cctccataaa
     1141 aaataagtta ctttttaaat atcttgttta tgaattcatt ccccaaaggc cttttaagat
     1201 tgatacagca gtccgttcac ttcttttaga cttgtcatac aagtctttta aatgatccca
     1261 tatcagacta gtttgtctat ctagcttcaa tccattaaac gctaatgaac ggttcatttt
     1321 ttctattact ttagataaag ctacagaaat ttttatacct tcacaacgcg catcttggat
     1381 ttcaataata gtatcatcaa ttagtttttg aatttcttgc ctatcaacat attcaatctt
     1441 agaaagagaa tcttttaaag ctactaatga ttcaacgatt tcttttttct cttcagacat
     1501 tttcttcctc cttctagtga cccgtaaagc catgtagagc agagtccatt gaaccagtaa
     1561 ttacactatc tacaacgcca ttccaattta ctctacattt tccatttttc agatttgtcg
     1621 gtccatacgg atagcctttt tgacattcaa tgttcggatt ataccagata ggattatacc
     1681 attcagccgc agaagcagat tcagtcgata caaacaatcc cacaccaaca aatccaatac
     1741 acatcacact atagccaatt agttttttca tctcgtatcc atctcctaaa aaatagaata
     1801 gttcttatag ttattcataa gacatatttt ttccgttttt gttttatcct ttctgtccta
     1861 taacaacttt tttcaagtaa agaatgacat agtttttctt aaaaaaacag tctgtttttt
     1921 tctatttctt cggtgtataa taaatatgag tgttcatttt ttattttaat acgcactccg
     1981 tacataataa atcttgatcg catcaatctt ttgagcttct tgatctactt tcaattgaag
     2041 taatacaaat tgagtaacct tattcattgt ttcttctcca aataacaaat attgatccgg
     2101 attctctata tcttgtgtca cctggtaaag tttcccagtt gttttcattt gtacatgcgg
     2161 gtctacatca atactattgt ctttaataca acgatctgtc atatagtccc tcacagcttg
     2221 attgcgttta taaacatctg tgaaattact ccattttcta gcaaaatctt ttaagaaatt
     2281 ttttgtcatt tcatctattt gcatttgttg cgctgtctcg tcttctaatg aaccaatttg
     2341 actgttactt tcagtacttt gaagagttcc acttgtttct tttactgctt cgattgtttc
     2401 ttctgtcgtt ctcaattgtg ttttttgttt ttcagaagaa aaccaatgac aaccagtaag
     2461 acctatcaaa cataaccaac taaatactaa aattatccct tttttcacgt ttctagtcac
     2521 tgcctttctt atttcacctt acgaaccaga ctacgaattt ttgtttgatt gtacgtgcgt
     2581 tgataccgag ccataatccg tccttgctcg gcattctgtt ccacactaga gaaatgttgt
     2641 ccgccatatg aaacatctgt tataattccg gtgtgaccat agattccagg agacaatgca
     2701 ccacctgctt gccaattcac cacatctcct gcttgtaaat cacttggttt tgggttcatg
     2761 attacttgcc aaccataggt tgtccagtta taatcttccc ctattaacat cgcaaacatg
     2821 tgtccagaac ccattaactg tgggccacct aattgagata cataccatgc cgtaccacca
     2881 taacattccc cattatttac ggatcgtcca atgatccgat ccaaaacctt cgcattgcct
     2941 ttactttttc ctgcaataag cgaagttttt tttgttggta ggctattcca atgcttgatc
     3001 gcaaaagtat catattccgt taagttatag gacgttatga tactcattgc tcctcgataa
     3061 tacggactcc cttcggtaat acctgctgcc gcatagcctc cacgacctag aatcaataaa
     3121 caacttccag gatctttatt gcccacacat tgattaaagt tgctttggct cgccaccata
     3181 aagccataat agtcataaca agcttttaag ttcggaaact tcatatagaa accaccttca
     3241 gaaccaccaa ttccacgtgc agaaccttgt gggtagggac aaccaggaaa ccaggtaatc
     3301 ccaaaaaagt tcgtatcgtt tcttccagat tgtgactgtc cataagctga ttcatagcct
     3361 aattgagaaa gaatcacact cggtaataac tgatacttta cagctgcacc aactgctgct
     3421 tgcgcatgtt catctgtgat tggaccacgt cctttaatct ctgaaaaaat cggtaatcct
     3481 tcggtatatt ccccattaaa agcgacagct agacctgtag cttctcctcc atgctcagaa
     3541 agttctcctg aaaaaaagaa tggcaataga aaaaagggaa gaataaagaa aagaagacaa
     3601 cctagacacc ctttcttcat ttcttcctca tcctttcttc gataatggtt tttggactgt
     3661 tcgtcctttt ggtaaagtaa aatcagttcc tggttttgcg tccaaaatcg gcagtgtttt
     3721 aggatccttt tctatttttt ccgattgctc tgttggctta tctaaagaag aaaatgcagg
     3781 ttgtaacgat tttggtgcgg aaatcgacgg tttactttca gacgaacgtt gccccggttg
     3841 ttgcacagaa cgatttcctg atagagtggc taccgacgaa ttaggaacag agtattggtt
     3901 caataactgc tgcattgctg gataactccc atttttcgcc tgatttttca cttggcgaat
     3961 ctgttgtgaa agagaaccag cagctgttaa ttgtggcacg ggtacttttc cagaaggcaa
     4021 cggaatcgat tgaccaacaa aagcgatacc tttatttaac aatggttttc tttgattatt
     4081 cccttgttgc ttactcaatt gtgccaatga ttgtacctgt tgttttggtc ttactgaatt
     4141 catggacgaa tttggaatca aaccatttga aagaccagaa ttccactctg ttttcgattt
     4201 ggtagaagac atttcaggct ctaccccatg ataacgaaca tgacgaccac tataaggcaa
     4261 ggcttgattt gaacgcccac ctagttcaaa gggattaacc acttgcctcc cttttaccga
     4321 ttcttgtggt gtacgttttt gttgaaactt ttgaaatcca tgtatcgctg cagaagcgag
     4381 tggattcgtt gctcgataca cattccctgc taatcctgca cctttgattg ccaaattttg
     4441 tccagcagtt gatagttgac caggcagttg tttcatttga gacaaagaat gacttgctat
     4501 gtttttcgtg ttgtttatag tttgatctgc agctcctaac cctagtttct ttaataaatc
     4561 tttccgtttt acaatgagta aaattgatag gagtgctttg acaaacatat ttcctaaaaa
     4621 accaaaaaca gtattcattg gaattaaatc gtctactatg ttatagatca aaaatgccac
     4681 caaaataatc acactgtaaa aagatttttg aaacatcaag cccaaaaagg actttaaggt
     4741 attcatgagc atataatcaa atactggaac aaaagaagcg agtaatgaaa aaggaatcac
     4801 aagtaataaa cacaacacca acatttgaaa gataaaacga atcatgccaa tcactacaag
     4861 tggtaatcca atacttagat taagcaatgg caccatagaa ccaacgactg ctttataaac
     4921 agctttagtt ggttgaatat agacacgata agccttcttt ttccctctat cttctcctgc
     4981 agcagtggtc acttttgtct caatatcaga taattcttca cttgacgctt ccttggctag
     5041 aaattcttgt ggttcaatct ctgcttgata caattcttct aaatttgttg tcccataatt
     5101 taacaatata tacggatcaa caacagccgc tttataataa agttggcgca ctttttgaat
     5161 ggcttccttt tgtgtcgttg gcactaaaac accattgaca tcttttgtcg ccaattcatc
     5221 tagactattt gtagcagaaa aaataaagcc ttctacctcc acggacaaat catttaaccc
     5281 tttggtaaaa gcttctcctt tactaggtac gttactacca tagccaaacc aaaccattga
     5341 aaaaatgaca acgaagagaa atcgaataaa caccattttt gcgtaatgtt tccctctaaa
     5401 tatacgaaga gtaaatagat ataacacata caagacgaca atccccgcac caataggatc
     5461 gtatattttt gaataaatag tatccgaaaa agtgaaaaac tggtgaatta actcgttcat
     5521 tgcagctcct tcatagagct tctcaatgac aaaatcaagg ccttgccaaa tcaatttact
     5581 taaactaaag aacatattcg tcatcatttt tagaaaatct gtgccatttg ccccaaataa
     5641 cattttgcct aatgaaaatt cactatcaga taaataagaa gaaaaggtat caatatgaaa
     5701 tttcccaatc tcatagatcg attgatccac ctgcttttct gccgcataca ccaatccacc
     5761 aacaaaaagc agtccgaatc caatcgttaa aaaaaacaaa acgcaccatt tcttttcctt
     5821 atgctttata taagccattg tttcacctca tttctattgc atgattaacg tatcctgttc
     5881 ttctacttga gagtgacttg ttttttccgt tgtttgtaat gcagcggtca tttcttcaaa
     5941 caaactgtgt acattgagtt ttcctacacg tccataggga tctaacatcc aacactgtcc
     6001 tttaatcatg tggctcattt cagctaagtt cgcttcattt acttccaaac gtaaatgatt
     6061 caaaatcttt tctcgctcac tgtcttcatc aaaagcaaag acgacaccaa aattcccttt
     6121 ttctttatct gaacctaagt caccaataaa ttgtgtatct aacactaatt gattcatctg
     6181 ggaacgcccc acacgtgcca ttgctgcaat aatactacgt cctgcgttcg aattattaaa
     6241 tacccatgct tctgtaaaat aaatcgctgt tttcttacta ctatctcgtt ttccaaacat
     6301 ttcacaaaaa cgacctaaac taatcatcaa acacaaagac tttcgatccg catctgtata
     6361 tagttctggt cggacattgt catttggtaa tttcaaatct tggatctcta aaatcgttgt
     6421 tttggcatta aagtctaatc ccgggttttc tccataagaa aatcctaaac gtaaaatcga
     6481 atcttctacc gtcaagcgta aaaactcacc atattctcga acctcttttt gttccatttg
     6541 ttctaaatag tcgaccacat gcatcaagcc aacttgttgt ccggcttctt tttgaactaa
     6601 aacttcttgg attcccttta ataccgcccc ttttaaaagg tgattggtac ctaacggact
     6661 taactcatca atcatatcct gggctacttg tttcgcatct actcgggata aacaaacaat
     6721 tggatctaat acaccataat tacggggatt tgtagcatct aacgtgacat aactcatttt
     6781 ttgcaaatgt tctacaaaca aagggtaatg tttctgataa tacgggtctt gaatgagttt
     6841 cgtaaaccat ttgcgctttt ctcctttggg atccacaaat aaactttgta cattcataaa
     6901 cgaactatac aaaaagagca agccaactaa aaaggtcttc cctttaccgg tttttccagt
     6961 tactgctaca tgaggcgcat caaacaacgc acctttgatt ccttccgcaa tggctagaag
     7021 attcaaaaag acaaaacgat ttgacgcata aattaattct tctaatggtg ttttttctcc
     7081 tcgagaacca ccatccccta atgtatcttg ccgaccaata taccagccgg attccatacc
     7141 taattctgat actgctgaaa acaaccattc gccaactgct tctacagttg ttcggtgctt
     7201 ccaatgtaac tgccgtccca atggttgccc taataaacat ttatagaaca attctcgttg
     7261 atcatttttt gcacggtgga tatggataat cgtatgaagt tgttgaataa ccgttgcact
     7321 attttgacga acttcttcaa ccgtttgacc gtatacaata aaacatgcga tccaatcgac
     7381 gacaggttgc ccattatcta ctcgttgctt taaatgtttt actaatcctc gaacagttaa
     7441 taagcgttca ctaggttctt catcattttg aaccaattcc ttatgctctg ttttgaaccg
     7501 ttccgatagc caattcagtt ttaaaccaat acctccctgt cctttcaagc gtggatagtg
     7561 aattttcatt cgcaattctg ctgggaaaga aagatcctga gcaaattgaa atagatcaca
     7621 atttctcata ttttcaggtg tttccccaat aggcaacaat gctaaataac tactccctaa
     7681 ctcatcttct aattgaagaa ggcctttatg ttccttcggt atacgtacat acgttccttg
     7741 attgtctttc agtacttccc aattattttg ttcttctact ttgtgagcca ttcctcgaat
     7801 atattgcaga cgattgagat acaacagctc tttttttact aagggacgaa tagaaaagct
     7861 ataaagtaat tgtgtcactt cttttacata aggatcactt tcagaaaaca aagtttccaa
     7921 atcatctact tctaatccaa ataagcgtgc taaacttcct gctaaatcta accagattag
     7981 atttattaca tcatgaaaac tctccaccgt tgctttttct ttgactttga ttcctaaaat
     8041 aaatgaagga cgatagactt gaccttctcg ttccaacaat gtcactgttt cttttgcata
     8101 atacgcacca attgcttgac tatcgggaga aaatcccttt cctaatgtgt caaacttcgt
     8161 ccgtaaattc attgttcgtg gatacatcac caactccaca tcttcataat ctgctaaaag
     8221 caacagtaaa cgagccatca tttcttttat ttcctgtgtc ttttttggtg tgttttttga
     8281 aattgttcgt ggaaaaacat gatagtaggc ccagatacaa tcctctccat ttaacaaaca
     8341 atttttcttg aatgctttta ctggttcttt tacaagcacc aatgaatttc acctcccaat
     8401 aaaaaaggaa caaccagttg attgtccctt tttctaatcc tctaaataaa catacttatt
     8461 cacaaaaagt aattcttcca atgcattgtt tgataccttg tcccagttat aatacacaca
     8521 atcaccattc atcatacgaa tcatataaca aaaccaatcc cataaataaa aatgcacttt
     8581 ttttccatca ggttttagtt ttgccagtgc taacacacat aaatagggga gtcctatata
     8641 aaaaacaact cctgctggtg gaaaaaaaga aatgacccaa ccaataaatg aacgtaaaaa
     8701 taaatattca aagattacga ccaacaaaaa agttgctgca ttgactaatg caactggaaa
     8761 tggaagacgc caattgcttg taatctgttg tacgcgataa ggttccgcat aaacaatcga
     8821 ataatcataa ggttcccaat ccacttcact atcctcctaa tccataaata atttttcaat
     8881 gattttacca aacgcatcaa atacacgtgt tggatcattt aagaagaaat aaactgcggc
     8941 acctgctgcg atagatatta atgcatttcc tacttttgat ttcataaaat ttcgcaatcc
     9001 cagataaaca accaccatta ttaaaaaata gccaccttgc gttaatataa aatctacaat
     9061 tcccttcagt gtcatttccg ttcttctcct ttcctatcca accaattatg tgttaatttt
     9121 tcaacataat actgatttgt ttttttcgta atgacaatcg tcatttgttc tttttgtaaa
     9181 agtgacgtat caccatctcg aaagacgacc gttgctttta caataaaagg ttttttcgtt
     9241 tctttataac gcaatacttt catctctact tgatcaactt tagctgtttg attgagtcct
     9301 tcaggttctt tcatcatgta tgacatatct tttgcattgt ttttcgcata cttatctaag
     9361 aaatcttcca caaatccttc aacatctttc gcttccgacg catcaaccgt ttcgtataat
     9421 gtgtcatcat aagtaatcat tcgttgtttt tctgtattct ttggtagtgc cgtaaaataa
     9481 ggataagcaa taatacgaca gcccttgttt gaaatagaaa agggaagatt gagtacttgt
     9541 gtgattgtca cacttgtttc tttcttactc tctggattca tcgagcgaat atcataagtt
     9601 actttataac tagcagttgg aatggtttca atttctttta atccaataaa cgaagcagca
     9661 gttaattttc gaacacctga aacacttcct ggttcttgaa taaagttttg tgcataaaac
     9721 tcttttttta atgtttcttg acgttgttgt tcttgttcat gattatcgaa tgtattcata
     9781 tacaaagcaa caaaacgatt cataaaggat tcaatttcag gtgtgtacgt taattctgac
     9841 cgactttgta cttctttctt ttgtaattgt tggatttcat ctttcaaaaa cgacacttga
     9901 accttcgttt tcatggattg aaaaaatgcc aagccccctg aaaaacaaag aaataaaacc
     9961 aaacaccaaa tccatttcac tgcttttttt gaatggattt tcttttgctt cggtactttc
    10021 tctgtataaa ctttttcttt ccgccgtagt tgtcgttttt tcataagcta ctccttctag
    10081 tatgttttta ctagtacgta cgattgacaa cgataagctt gtttttctga aaaatagtcc
    10141 attcaattac cctcattttc tattcctttt ctctttcttt tatatccaac aaatccagca
    10201 atacttgtca acactcctcc tagaacagaa agtgcaacat gtatttcttc accagtcttt
    10261 ggtaagactt ttgtttttcc atctgctgtt ttgatttcaa tattgccact tggaagtttc
    10321 tttacaggaa tcgacaaatc ctttaccaaa ttcccttcac tatcttggat taatggaata
    10381 ccatctttta caccaacaat tgtttgatca cctgctactt ttaatggggt ggttggtgtt
    10441 ggaatatggg tcaattcctc tacattacta gtgacaatcg gtacactcat tccttgagaa
    10501 gtattcgtcg taatttcacc gcttggggtt accgatactg tgacttcttt tgctggaaca
    10561 ataggtttat tttcttttgg aattgcttct gatttttcaa caggttgttt cggttcttct
    10621 ttttgttctt cactggtaga cgacatcgtt ggctgttccg tacttgacga atcagtattc
    10681 ttttcagaag aactggttgt tcctgattgt tccggagttg tgactatact tgaatccgtt
    10741 gttgttgaac tatgcacacc ttcttcttcg atcgaatcag tcggtaaagt tgattcagga
    10801 aactctggtt ctggtgtcac tggctctttt tcaggtttct ctgactgcaa ttgttcaggt
    10861 tctgctggtt tttccgttgt tcctccattt agctctgtct caacatctgg taatccttcc
    10921 cctggcaatg tttctacaat ttctgactta ggtgtttcta cagtttcatc tgcataaacc
    10981 aatgaactcc ctaattgaaa cgaaaataat gaaattccta gtgttgttac gactgctaat
    11041 gttgtttttt tcatgattat tcctccaatt ttttatgcaa ttcgtgtttt gttcatagac
    11101 gccgcactaa tattatctga acgataaaag aactcccgtc cgttttcttc taacccaaca
    11161 tatggattat ttttgaataa ttgaatcaat agttcactgg tgttgtattt tggatagttt
    11221 gcaaaaaaat aatactgtgc ttgtcgccca tccgaaaatt caacaagtaa ttcataacga
    11281 taccgcttca atccttttcg ttgttttaaa ggatctctat accaacttgt ccatcgttcc
    11341 attcgtggat agagatagac acgatacaca tagaacaatg ccataccaca taccaccaaa
    11401 aataataatc gccacataaa aattccccct tttcttctac ctttcttgaa tcatcgaaca
    11461 ttcataccct ctatagagat ttgaaaaatc catcatcaat tgttcatata aacgttgttg
    11521 caaatagcga ttgtttcgaa tacataattc ttcatagcca tcaatcactt gatcttcttt
    11581 ttcatagcat cttgtagctc tatgacgctc agcttgcatg acataaccaa actctgcacc
    11641 atatcgttct actgttaata actgttgact acgcaataat gcaatataac aatcacaaaa
    11701 atgttcaacc gacatttgat ttttctcacg tttgtgaaat aggtattctt tagaaagcac
    11761 attattcgtt aaacaatctg ataaatagcg ataatctggc ggtcggttat tttcatctgt
    11821 gaatttttcc cataataaac tgatttttat tcgttcagcg gctagatagt cttgttcgcg
    11881 tggataaaat tcatcaatcg cacaaagttt tttatcacct gaataaacaa atccatataa
    11941 tgcgaaacga actcttcctt ttcaattggc tctcctaaaa attccagtga cataccatct
    12001 tctccttcct aaaaaaaata aaaaagcaga aaccgatttg gcttctactt ttctttcctt
    12061 ttatagctct tttttacgga agaatccgca aatgacattt tttcaaaaaa tcaatcggca
    12121 caagttcacg aaaacttgga gcaattttgg atagaatcac ttccccatct tgtaaaatac
    12181 aatcataaaa atcatcagtt acttgatact tacgcttctt acaataattg ataaaatagt
    12241 agcgatcatt ttctatttcc attcgttgtt ctactaagcg catacgaaca tcatcataga
    12301 aataagccac aaatcgccct tcaagttcat gtttttcaat gggatactct tctggtaaca
    12361 atctattttt tcgtcgttta tacattgctg gataccttag aagaagttca tctaaacgtc
    12421 tgtcagtata tggtaactga cgaatcaacg attctaatcg ttctttatac agatagaata
    12481 attggcgcaa gcaatagtgt accgcctctg gattttctaa tactgttgga tcacgtgacc
    12541 aatacgcacc aacataaatc ataatttttt tcatttagac aatcacacca gcaatactaa
    12601 tgaccgatcc acaataggta gaacaagtgg catagcgacc aatttcctct aattctatct
    12661 caatctcttc taaagaaaga tatgtctgtt cttctgttgg ctggcgactc atttcccaag
    12721 tgacagtcac caatgaaatt tcttccataa gaggtttttc gctgctaagt gctgccacac
    12781 agttttcata tatcttcatt ttatcgcagc cttttttaac cgctaaaatc gcaggtattg
    12841 gcaatgaaaa tcgataaaag cgaatgaatt ctagccttcc tttccttcgg ctaaattcca
    12901 actcttcgag ttcttgttcg tatcccttac gaacttctaa taagcgtgca atttcttgcc
    12961 agttctcttt ttcctgataa cgtccagctt ttaccgcttg ttcaatataa ggaactcgaa
    13021 aacgaaaata agacaaatca accatctttt catttaatgc ttcgattgca gcctttaaat
    13081 ccaacaggcg cagccattct gttgtcgtat atgcattcat gcaattttcc ctcctgacgt
    13141 tcccatccat tctagatagt tttgttgctc gtcatttagt tcggcattat caatcccttc
    13201 ttgtacaaag tctcttccta aaatacgatc tgcttcttgt aacaacttta atgtcggagc
    13261 tacttgtttc aaaaaccagc gttctgaacg atctaatgag aatttttctg gctttactgt
    13321 aaaatccaat tcttccatcg aacgaaataa ttttgcccat cgtttatcaa caatccgtgc
    13381 atcactttcc agtctatccc aaacaataag ttttgcatta attaaaccaa cgccaatctc
    13441 tcctaaacgt ttcggtgaat cacctaaaat aaatgcttct actgcttgtg ttgctttctc
    13501 attggctaac cgtaactcat atcgattata aataccatat ttcgttaatg cttctaattc
    13561 tgaaattttt tctcggttcg caatttcaaa acgtttctca taaaaattaa aaaagagagg
    13621 actctttcgc gatccaaaat acaaagataa tccttcactt cgaaaatagc catcatctaa
    13681 aaaacaaccg ccaatattct ggaatctctt aaagtccatt tctaataaac ctgctttttg
    13741 tttttctagt aattcaaaga gatcgaaatg ttcttccgtt tccaatcccc aaagttcatc
    13801 taacgcaata tcgaatcggc tacattgcac acgtgtataa ccagcttcca actttccttg
    13861 atttttccaa atggaatata gccatgttcg ccaatctgtt tgttgttcac gcatgacttc
    13921 ctcatattcc cggcaacctt gtcctgaaaa ttgcacaagt acattttctg ttacagttcg
    13981 ttttgaatgc cagaacaccc agatattccc acgaacagaa agacagtcaa aaagatacaa
    14041 gcccgttgga cgtgtttcaa attctgtcag atccattcgt aatacacgtt gaatcactgt
    14101 tttgtagtct gtcccaaaaa aggaaagacg caaataatca atcattgctc gaagatctat
    14161 tccgcttttc atatacaatc gaaagcgttc cttgattcgt tgttcaaaat cctctgggat
    14221 ttttcttttc ccttgttcca tctttctaat gtaatctttt gagcagccca tcaattgcgc
    14281 aaatcttgct tgtgatagcg gctttttatc tccacttata ttatgaattg cacttaactt
    14341 ctttctgtag ctttttagct caatcggtgt cattttctcg ctccttttat aaaataacaa
    14401 aatcgatttt ttttaaccct ttagaaacac cgatatatca actagtcccc ctcgtttttt
    14461 gggaaaagta gtacaactgc tgaccttaga taatcacttg atacaaaagg gtttataaaa
    14521 cttttttcta tgtgttatgt tatttctgcc ccccatatta tatatcgggg ggctttacac
    14581 aaagaggccg tgcgcaagcg cacgacctct ttgtgagctg cacacgctgc tagcgagtgc
    14641 gcattatagg cccatgcttc gcagccttaa tgcgcaccgc caggcagagt gcggctacca
    14701 gactacgtct agagccctcc cgatagataa taactacaaa attaaatgat ctaattttgt
    14761 tatttcacta tatttttcag gagaaaaaac gcgataaatt gacaaagtta aatcctcttc
    14821 taaatctact cgataatgat acaatcgagt caatcgttct atatcttttt tcgttagcaa
    14881 tacttgatta ataacttttc ctgtaaaaac acgttctttg cgcaaaaagc ggtaatcagt
    14941 tacttgttca atacgattca accaatcata aaacgttctg gaagaaagag aaggattaat
    15001 cattcgtatt gctgtagcta ctctataata acaactctct gccatttaac ttcacctcct
    15061 tagttgatta caaaatatgc gcatcagaaa aacttgttcc ttttggatct tttgctaaat
    15121 acatttggcg ctctagttgt tcttgtaaca actgattatc atctcccatc gtttgatctt
    15181 gactatcctc ttttactgta aaatcataga caatcatttc agtaaatttc tcaaattctt
    15241 tgataaagtg ataatctttg taaacaaatg gcgcaaaaaa ctctacaggt acatcaaatc
    15301 ctggaaaagc atatccacga ccaggttgat ctttgttgta aaatggctta tttttaaaat
    15361 cttctccaaa agtcattaaa taaccaatgg gagacaatcg accaacagaa atacggaatg
    15421 ttaaattatc ccgaacacca cttggaaaat attctgcatc tggtcgttgc attgctaaaa
    15481 taatgtacat ccctgcttga cgacccttta atactaatgg ctgaatcaat ttatcgactc
    15541 tatttctcgc ttccatatcc aacgaaccat aaaaagctgc ccattcatca aaaaagataa
    15601 aatgcggtgg taacccataa taagcataat ttttccctgg ttcatattta ggatctttca
    15661 tggttttcat ataggcatac cgttgattca tttcttttaa tgcatttcgt aaacaaataa
    15721 tcatttcttc atttattttg taaaacacat gtcctttgaa tacatcgacg tcacctaatg
    15781 ctgctaaatc tgccgctttt ccatcgcaaa tatctactgt cccaacactt aaacaagctt
    15841 gaattaaaga atacaataaa taggttttcc caccaccgat tgcaccagca atcaacatat
    15901 ttggtgattg atcatatacc caatccaaag ttttcattaa atgaatacgt ccattttctg
    15961 ctatacattg atcaatagga atccgaccag aatctgaaag gaatacataa ttgattcggg
    16021 ctttttctcg ttcttcacga attaaatcac gcaaaaatac ttcagataac aaatgaccca
    16081 tttttaaaaa tcgttcgtga aaacgcccac catctaaagg aaaagaaacc actaattctt
    16141 gtttcttttg ataaaaataa acttttggta atctcatttt ttcccttgtt tgtttctcac
    16201 taacttgttt ctttgtataa acaaaccgat tgtcaattaa cattcgaaca agtaactgac
    16261 gccaataaac agaggcaaaa tatccttctt ttagtcgcga ccaatgcatc aaccctaaaa
    16321 ttaacccact aaccagcaac aaatacaaca caatacttat tccaggaata agatacattt
    16381 gccaattcga aaacgtactt aaacgtggat aaaatacttt ccaactaaac caacctaaca
    16441 gaatcaaaaa tggaaacaaa aagaaaccat aatagcaaaa ccataaataa cgattgaaat
    16501 agcgaattcg ccgaccacga tacttgaaaa aatgagcttt caattgtcac attcctcctt
    16561 tctgttttta cttttcctgt gctaaaaact tttgacaaaa agcaactaat acaggaatat
    16621 cacatttagt aaaaaaacat aatcgaaaaa gatttaatcg atttttataa gggtgtgcac
    16681 acatattaca tattcgtact atacgaatcc aaatcacttc ctgatgatct tgtttccact
    16741 ggtacggtaa tgaaaaacga acaagatcac gtaaaaacat acactgccca ataaccgcac
    16801 aaatatatgc atttgtttgt acaaaaatcg ttggattgtc tccaaaatac tcaatcaatt
    16861 gttggatatt ttgataacaa gcacaatctc tcaaagtatt tgattggttt tatctgaatt
    16921 caaaatctcc ccttccttct atcttagata aaaagaatag gaagactcct tcctattctt
    16981 tcaaaaaaac cttatttttt ctctttttcc ttagaatcaa cacttggttg tttttgatct
    17041 aaaccttttt cttgtgattg tttcatatta ggattatttg ttggagcttc tactttctct
    17101 aatcctttcg cataaatcac taaatctgaa tattcacgat tgccatcttg tgctggaata
    17161 tataccacat gatattctgg atcaattaat ttcacaggat cacgtcgttt aaaaccagtc
    17221 aaatctacat ctgctggaaa tttaactcgt cgtgcaatct ttggttcatc tggatttcca
    17281 aaaactaatt gtgctcgctg tgatcgaatt tctcccgtat aatcgcctgt ttcattatca
    17341 aatacacgat cagactcttc taatgcgcca aagcgtacgg ctcctaaaca agtttcatct
    17401 aatgcttctg taatgtcata attatttgga aattttaatc ccatcttaca aaacctcccc
    17461 aatttttaca ccagttaaaa agacaccaac atcttgatag ccagttgttc gacgctctgt
    17521 ttgtcccata tcatttgttt cacgactatt cactcgttct ttacgaaaaa caaaataagc
    17581 ttcttcaaat gaaatacgca cctctttttc tggtaatcgt aatcttttat cctcttcagc
    17641 tgtgagataa acaaaaaaag caggctgttc ttctcgcgaa cactctgctt gtaattgaac
    17701 atatcgccca gacacaccat taatttcctt tggtggatta ggttcccgtg aacccaataa
    17761 aaatacttga cctaaaaact ctttcttcat acgcaatgct ttttctgcca taaagttttt
    17821 cctcctctta aaaaccaaaa taaattctaa atatttctac attacaatca aacaagcggt
    17881 taaaaatcct aaccgcttgt tcctcatgac taatctttgg ctttacgtac tttataaagt
    17941 gttataccta aagcaatgac taatactact aatccaagaa ccataagtat actacttgtt
    18001 tgttcacctg tttttggcaa cattggtaca acagtagctg gtttaacccg ttgttctggt
    18061 ttcactggtt gcggtggttt tggcgcttcc acaatttgaa ccgtttgatc tttgtttgtt
    18121 agatctacat tgtgtttcgc cacttcttca aacgtttctt tctcatcttc cgttttcgct
    18181 tggagccatt ccgcaaaact caagcctttt cctgcaagat tgcgaccgtc cactaagaat
    18241 tcaaataaga attccatcat ttctttatcg gccgcaaaga ctttttccgc atgagtgcct
    18301 tcaaatggtg tgccgtctgg tgttaagtag ccttgaacaa ttaagcgata gatttgacca
    18361 atcgcaagac cgtctacttt gacccattca agcacagttt gatttggcaa ggccaagatc
    18421 tcttttccgc catttttatt cgtcgctttc gtcgtaatgg ttggattact gattttgatg
    18481 gtttggtcat tgtcggtaat atctgcgtga gaagccaata atacgccttc tctaaacaga
    18541 tcttcaaaga ccacgacttc tttgcctcgc aacgcacgtg cgtctagaat aaagtctaga
    18601 ttgactgtac catccgttgt ttctgccaca aacgttaagg tacttgtgac ttcttttcca
    18661 tctactagca ctggtttttc cgttactttg tccattagaa caccgttgac tgtgtatgtt
    18721 tttccaacga ccaaatcgtg gtatttcaca gcgtctgtaa tggtgacttt cgttaatgga
    18781 ttaacctctt tcaagccagt gggtttatgt gtagccgttg tacgtacact tgggttagtt
    18841 actcgtaccg tttgcccttt gtcgttgatg tctttatggt ttgctagttc ttcatcattt
    18901 ctaaaaagac tttcaaacac gacgatttca cgaccattta tacctgtcaa atcaaaggta
    18961 aatggtacga tcaccgtttc attttctgct ttggcaataa agccaacact cgtttctacc
    19021 gtatttccat gcacaagcaa gggttcaccg gttgctttat ccattaagat ccctttgacc
    19081 aagtatgctt ttcctggaag aatctcggta atttcaacgg tatcgtttaa ggtaaacaat
    19141 cctgttgggt ctgcgactgt caatccgtct tcaaagacag ccgtggtatg aacggttgga
    19201 ttattcactt tcacactttg ggcttcatcg ttgatatccg catgtgctgc aacttcaatg
    19261 ccatcacgga aaagtttttc aaaaacaacg atcgtttgac ctttcagaac actagcatta
    19321 accacaaatt caaggtcttt tgtgccattg actttgtctg caataaaggc caaggtactt
    19381 gtgatttctt tgccatcttg taaaattggc gcattcgttg ccttgttcat caatactcct
    19441 gaaatggtgt attcttttcc gacaaacaag ttggtataag aaacactgtc tttaatgacg
    19501 atcgtttcta atggatcaac cactttttca ccatcggctt tatttgacgc ttgtgtttct
    19561 agttttggtg gaatcaagta attacgtaca gaaaccacta acgtttcccc atcatctggc
    19621 gcattttgtt tgaatgcaac cgtgaatgct tgtttatctg tatttggttg gaaactttct
    19681 aatggggcat tttccaagaa gtaatagtca tcggcaacta gacctttcac taaaatttcc
    19741 ccgttttgat cagtggtata ttcaccaatt gcttgatttt taccgttata caaggtaaag
    19801 ttcacacctg cgagcggcgt tttcttcttg ttccattctt catacttgaa taattttaca
    19861 cttttccgtg cttgacggtt cgcaaaatgg atcactttga tcggttctgt atcattggta
    19921 atcgagaaca ctaatgggtt ttgattcaaa acgtaattta cgggggcttt gatttcttcc
    19981 aaacgatagc gatcttttcc ataagtaagt gtaccgttag ttaggaaaaa gcctttttca
    20041 ttggttttaa agatatccgt tgtttctgta tcatttggaa tctccattgt gacataacga
    20101 ttggctaaag tatcaaaaat cttgaattct gtattagcaa gtggaatcac ttttcctgtt
    20161 tcttgatcca ctttcaccac tttaagacgt tgttcaatta ctttatcttc taaaacataa
    20221 tggaaagttt gcccattttc agaaattgtg acttcaagtg gttcaaatag tttataccct
    20281 tcttttcctt tggtttcaga aagaatgtac gtatcaaaga caacggtatt tttaccaaaa
    20341 gtagcataac catcattgtc tgtcacaacg gttttcacca ctttgccagt tgtttttgac
    20401 gtcaaagtga tttcaatgcc agctaatttt ggtttgttat ctggattcgt tgaatcgact
    20461 aaatctttat tagcaacttt cactaaatca atggttcctt tgatcacttg atcttttgcg
    20521 gtcgcattga cagtttttcc aggttcaaca acaatttcaa ttgcttcatc agaaagcaca
    20581 taaccttttg gcgctttgat ttcttttaaa acatatttgc caggatcaag atctttaatt
    20641 tctgcctgta ctttgccgtt aatttttttc aaggtaattt ctttaatctt tttcccatct
    20701 gcttgataca aaccatagac cgcattttct aaagaagctg caccttgtgc tttcacacca
    20761 gtttccgcat cttctttaaa cacacgaatg tttccttgtt ttagaatttg taaattaata
    20821 acagacgcat taggttctgt tggatgtaac acaccaattg tttgagctcc tggacgacga
    20881 taggctaatg gcgcaccttg aactttaatt ttattcaatt gaatttttgc tttatccggt
    20941 gcctcttttg tgactgtaat ccatacttca ttgccgtgaa tagatacatt gacacctttt
    21001 ggtttttgaa tgtctttcac atatttcaaa aaatcatttt tattctttac tttcaacgtt
    21061 tcgccaattt ttaccgtatg tgtttgatta ttaaaagttg ctttttcacc aaagttatta
    21121 atcaaacgtt gaatttctgc ttttcgacga tcataatcaa cacggatatt tttgattggg
    21181 atttttgtgc ccaaagcttc ccaaatcata aattgtgccg caatgtattg ttcatcggat
    21241 ttgtctttat ttgtgacata tcctaaataa gagatcaagc ttgttttttt ccgtacattt
    21301 gaatttttca aatagttttt caaagcagta gctgtataaa cttgaccttc tgcggctgag
    21361 acaccaggct caatacaaaa cgccacttgt ccattaatct caaaacgaac catataattt
    21421 aaagtgaagt ttagattgtt cggactagaa gtaacaccaa tattttcgcc ccaatcaatt
    21481 ttccgaccac ctaaaacagc tgcattggtt gcacggcgaa cacgaaccga acgattggtt
    21541 ggtactgtta ctaacagtgt aggtgtacct gtttttggaa tgttctgctc ttttttcttt
    21601 tgattttcaa tactagccgt agattctttg tttggtttcg ttggttgatt ttgactagct
    21661 gcggttgaag agattgtttt ttcaacgttt tcctcatttt ttccttcttt gtcagcagta
    21721 gccgctggtt gttccactgt ttcttctatt cgtttctttt cttccgcttg tttctcagaa
    21781 gactctggct gcacagaagg cgttgcttcg gcagcttttt ctccttctag agtggacgtt
    21841 tctgaagcag gggttgtaat tgaggcagtt tcttcaactg gttgtgaagt accaggacta
    21901 ttccctgggt gttgtgtttc cccaatcgtc tgatcgagag tcgttttgga attgaccgtt
    21961 ggcgcttctg tcacaggggc ctgttcaaca gtggcttcac cagctgggac cccggcttct
    22021 gctggttgga cttctgtcac ttcagaagcg acttgggcaa tggcttccat cgtaggttgt
    22081 atagccgtcg tttcttctgc atgtgcttga gcagttccca ccaagaaaca gaattgagaa
    22141 aaagccacca atgcgagtgc tttcaaccaa ttcattttcg tttttgcata cacaagaatt
    22201 cctccttttc ctgagaattt tatataataa aacaaaacgc cgttaccaaa agtgataacg
    22261 acgtgaactg tctcttctat ttttatctgg cgctcttatc tgaacacatt tcatcaatga
    22321 ttttaccctc ccactataat tacagaacaa aaaaagaacc tgcttcgtgg aaatcaactg
    22381 tccatttccc aattggaata ccagcttttt tggcaacttc accatcacca aatctcatgc
    22441 cataccaatt actaccttca tccttaattt ctttccaacc atatgacata gcttcatcta
    22501 atgaatcaaa aatcatgaca ctattgccaa ttgttacagg atctggctta agatcttctg
    22561 gaagagaaat atcaatcata ccataatgat ccgattcctc agtattattg tcattactag
    22621 ctactggttt ggttgttcta ctggcttatt ttgatacaac aagtactctt attcttcttt
    22681 tacttctccg ttttcaattt cccattcaaa ccactccgca aagtcttgct taaaaccatc
    22741 tactcgatac ctattttgta aataggcatc ttctgacgtc gtaggatcta aataggtagg
    22801 tttattctct ggacgagcat agacacctac ccatgtttcg taatctcctt caaaataact
    22861 atctaccaaa tagccttttt ttctaagaat ctcccgaact tgatttttaa ttattgatac
    22921 ttgttcaaaa ccatcactca ttctatcacc tcaaataact ctagattctc ataaatattc
    22981 ccgacaattt caatcctatc aatgatctct aacaaatcgc tacttatgct tccccattcg
    23041 tataagaatt tcccttccgc aaaataaatt ttccataaac ttcttcatga tcgtcccaac
    23101 caatatcact ctcgaaaaat tccacgttgt tcttatcttt tagctcagta aatgccataa
    23161 gttcaacatg ttttccaacc tcgaaacaat cacaccaccc atcaaccgtt acacgtgtgc
    23221 cttcaatacc taagaaactt tgtcctcgtg tattaaacac taaaactttc cgcatttttt
    23281 gctcattaat taatcacgcg cgaaatctag gaatcacaac ttattcgctt cttctttata
    23341 aataaaaaaa ctccaataat cggaaattac tatcttttcc tcattccttt tttaaatctt
    23401 catacttctt cttatctttg tcctaaccta ataacacaaa ttttgaagaa aaactcagta
    23461 aaacctgatt ctttttttat cttcacaaca accaactttt tcttcagatc cttttactag
    23521 aacttgtata caaaaattgt tgaatgctga tataatttgc ggctacttga aaaaa
//
LOCUS       DACDIG010000043        22987 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.043, whole genome shotgun sequence.
ACCESSION   DACDIG010000043 DACDIG010000000
VERSION     DACDIG010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.043"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(111..566)
                     /locus_tag="IV247_001741"
     CDS             complement(111..566)
                     /locus_tag="IV247_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777812.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(720..1928)
                     /locus_tag="IV247_001742"
     CDS             complement(720..1928)
                     /locus_tag="IV247_001742"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAP8777813.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2040..2372)
                     /locus_tag="IV247_001743"
     CDS             complement(2040..2372)
                     /locus_tag="IV247_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP8777814.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2520..3395
                     /locus_tag="IV247_001744"
     CDS             2520..3395
                     /locus_tag="IV247_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8777815.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3477..4058)
                     /locus_tag="IV247_001745"
     CDS             complement(3477..4058)
                     /locus_tag="IV247_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAP8777816.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4064..5323)
                     /locus_tag="IV247_001746"
     CDS             complement(4064..5323)
                     /locus_tag="IV247_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP8777817.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5325..6371)
                     /locus_tag="IV247_001747"
     CDS             complement(5325..6371)
                     /locus_tag="IV247_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777818.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6606..6881)
                     /locus_tag="IV247_001748"
     CDS             complement(6606..6881)
                     /locus_tag="IV247_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAP8777819.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7124..8434)
                     /locus_tag="IV247_001749"
     CDS             complement(7124..8434)
                     /locus_tag="IV247_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAP8777820.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8616..9845)
                     /gene="rpsA"
                     /locus_tag="IV247_001750"
     CDS             complement(8616..9845)
                     /gene="rpsA"
                     /locus_tag="IV247_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAP8777821.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9980..10660)
                     /locus_tag="IV247_001751"
     CDS             complement(9980..10660)
                     /locus_tag="IV247_001751"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAP8777822.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10709..11344)
                     /locus_tag="IV247_001752"
     CDS             complement(10709..11344)
                     /locus_tag="IV247_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8777823.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11410..12813)
                     /locus_tag="IV247_001753"
     CDS             complement(11410..12813)
                     /locus_tag="IV247_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAP8777824.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12800..13831)
                     /locus_tag="IV247_001754"
     CDS             complement(12800..13831)
                     /locus_tag="IV247_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777825.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13862..14083
                     /locus_tag="IV247_001755"
     CDS             13862..14083
                     /locus_tag="IV247_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAP8777826.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14248..14985)
                     /locus_tag="IV247_001756"
     CDS             complement(14248..14985)
                     /locus_tag="IV247_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAP8777827.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15138..16478)
                     /locus_tag="IV247_001757"
     CDS             complement(15138..16478)
                     /locus_tag="IV247_001757"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAP8777828.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16715..18238)
                     /locus_tag="IV247_001758"
     CDS             complement(16715..18238)
                     /locus_tag="IV247_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAP8777829.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(18616..19227)
                     /locus_tag="IV247_001759"
     CDS             complement(18616..19227)
                     /locus_tag="IV247_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8777830.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(19350..19475)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(19557..20273)
                     /locus_tag="IV247_001760"
     CDS             complement(19557..20273)
                     /locus_tag="IV247_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP8777831.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(20279..20848)
                     /gene="scpB"
                     /locus_tag="IV247_001761"
     CDS             complement(20279..20848)
                     /gene="scpB"
                     /locus_tag="IV247_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAP8777832.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(20832..21629)
                     /locus_tag="IV247_001762"
     CDS             complement(20832..21629)
                     /locus_tag="IV247_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAP8777833.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(21599..21970)
                     /locus_tag="IV247_001763"
     CDS             complement(21599..21970)
                     /locus_tag="IV247_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP8777834.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(21989..22876)
                     /gene="xerD"
                     /locus_tag="IV247_001764"
     CDS             complement(21989..22876)
                     /gene="xerD"
                     /locus_tag="IV247_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAP8777835.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         6448 a         4911 c         3900 g         7728 t
ORIGIN      
        1 aaaaatcttg tcacagtccc ttattctgaa taaacggtgt ggaagagaca gcctatcagt
       61 agtaataaga cacatctctt tcacagcctc atttttgcgt aaaaatcgat ttattcgaaa
      121 aattccccgt atacatcttc tacacgttga tctggattga tcacgatata caagttacct
      181 gttttttcgc tgatttttgt tgattgatat tgattgtcta atccttctgt tcctttatct
      241 agatatggga agtacacaag atcaggtgtg ccatttcggt aaaggatttc cattcgctcg
      301 atatcttcgt atttcaatgc gcgattaaac atgcccattt ccagtccgcc taggttgata
      361 tcatgtgtcg caactgcgtc cccttcatgg aagatttcga ttttaaatcc agcacacgga
      421 tggatttcaa caaattcact gccattgatt cgtccaaaac ttgttgtgat ttgtttgatc
      481 caaagatcgc cgatatgacg gcggttgatc gtccaggttt ctccatttct aaaataaaat
      541 tttagcgctg tcatttcttt actcattaaa aagactcctt tatgatgtaa tttattcttg
      601 tactacctca gtttagcaca aatttatgta agtggtcaca tcttatgttt tttcttctaa
      661 tgacgtatct gcccacttta agtagccaac aaagctgagc agatacagtc tagtgttttt
      721 taaatcaatt tttctttcga taaaaaatcc aaaacctgtt ttttatcatt ttttattctt
      781 tgttctacga cggctttttc agctaattgc aaagcttcac taacccactt accaggtttt
      841 tttccagttt cttctagcaa gattttacca tctactgcta attcgcttaa tgacttgata
      901 ggtaaacgat cataggattc tcctacggca tccatctgag gtgtttcgtc aaaatagatc
      961 atcaattctt ctacttgcag tgcatgtttt ttgcctaaga agtacaattc gaatggttcc
     1021 aaagctccag tttctcgttg tcgtagtcct tggacaagtg cttgaacgtc tttaatcatt
     1081 tggttagaac acttccacga tttcaaaaag gaacgaatct cattttcttt tagatccagt
     1141 tggctgatca ataatgccca tgcttggctc tcttctttca atgggacgga tggtaattca
     1201 gcaaaacgta aaagtgcttc gccttcttct ttcaaaccgg gacagcaaat gtagcactct
     1261 gtttcaataa atgcatggat tcccgcggcc cgttcatttc ccaagagcat cttgatgaat
     1321 tccacattta tacgttctac agatatttta cctaatagct cgtggttttc tttgatggcg
     1381 tagaaggttt cttcctctaa tttgaaccct agttgactga caaaacgcaa cccgcgcatc
     1441 attcgtaagg catcttcatg gaaccgctca tgaggattgc ctacagctcg caagatctgt
     1501 tcttctagat cttgcagacc atcgaacaaa tcgatgattt ctccatcttc tttcaaagca
     1561 aacgcattga tcgtaaaatc tcttcttttc aaatcttctg ccaacgaacg aacgaattcg
     1621 acatgatctg gtctacggta gtcctgataa gttgattccg tccgaaatgt ggtgatctca
     1681 tattgttcat cattttctaa taccaacaca gttccatgtt cgatcccaat atctatcgtt
     1741 tttggaaaaa gctgtttgat ttcttctgga aatgcgcttg ttgcgatatc cacatcatgg
     1801 attggctggt tcagcaagat atcacgaaca cttccaccta caaaataagc ttcatatcct
     1861 gcattctcta attttttcag aactggaatt gccttttggt attccagagg taattgtttt
     1921 aatttcattt ttccaacctt tcctgcgtgt ttcttgtcct gagataaaga ctcagttgca
     1981 tggtgactgt ttgacaagtc cgtttcagag tcttcaaggc caggaccact tcatcttgtt
     2041 tactcgtcct tacgctcaaa gcgattatgg tctctttgat tgaatttttc catgtttttt
     2101 tcaaaaactt cagttaagtc gatattcaat gaattagcca tgatcatcgt aacgaataaa
     2161 acatcaccaa gttcttctgc taccgagtta actggttcag aagccttttt gcttttttct
     2221 ccataagtgt ggttgacttc tcttgccaat tcacctactt cttcagttaa tcgagccatc
     2281 tgagctaatg gagagaaata ccccaccttg aattgctgga tatattcatc cacttcctgc
     2341 tgcatactgt ataaagaacg ctttttatcc atctttttcc ctccaaaact atcttaccaa
     2401 aactttaacg caaatcctag ataagagcaa ttgagaaaat cgaatttcca tgctacattt
     2461 aggaaggaat ttctaaatgg aaattctgtg aaaatacata attgaaagct ggaattcata
     2521 tggtcaaacg aaaaacagta aaagaagttc tttttatcat tataggaaca agtatttatg
     2581 catttggtct tgtttacttg aacatcgcta atcatttagc agaaggggga gtctcaggga
     2641 tcaccttgat cttaagagct ttatttggta ttgatccagc ttatacgact ttattaatca
     2701 acatcccatt gattttacta ggaggaaaga tcctcggtaa acgttccttg gcctatactg
     2761 ttctaggtac actttctttg tcattctttt tatggatctg gcaaagggtt ccactagaaa
     2821 tcaatttaca gcatgatcta ctgatcgttt ctctacttgc gggtttagtt gcaggtgtag
     2881 gaagcgggat cgtctaccgc atgggtggaa caacaggcgg cagtgatatt atcgctcgga
     2941 ttttagaaca aaattacggc atcactatgg ggcgttccct attggcattt gatatcatcg
     3001 tcttacttgc ttcattaacc tatatcgact tgaaacgtat gatgtatacg ttgattgttt
     3061 cgtatgtttt cagtcgtgtg attgattcga tcttagatgg cggatattct gcaaaaggtc
     3121 tacttgtcgt ctccaacaaa agcgaagaga ttgcaccact actgatgacc ggtctgcaaa
     3181 gaggtgtgac tttcctttct ggagaaggcg cattctctgg taattcaaaa aaaatcatct
     3241 atatggtcgt tagctctagt gaattaactg aagctaagag gatcgtccac gagattgacg
     3301 aaaaagcttt cctttcaatc atcaatgtgc atgaagtgga agggcaaggc tttacttatt
     3361 tgaagccaca gacaagatta ctgaaaaaac tataagaaaa aagacaaaga taggctatga
     3421 ggaaagattc cacatccagc tgacctttgt cttttttagt actatactat ttttaactaa
     3481 ttgttatgtt cacttacccg gttacttttc ttttgtgtct gcaaaaggat ctttcggtta
     3541 acttcatcag ataatttcaa aaaagcatcc ctatctccat cttctagtgc ttgatcgatt
     3601 tgagcataaa gcatttttag ttttgcctct tcttcttttt cttgaaaata tcgttcaatt
     3661 aattcagtat cttcttcact gactgaagaa tcccaagaag aaaatggatt gtcttctaag
     3721 atagatagat attctcttgt ctgccaagca ttttcgaata tacattccac atataaaggc
     3781 tttttccaat tcaaacggat ttcatgaaaa atctggtctg tgtccgtaaa ttcttttccc
     3841 tgcaaaaaca atttcatggg ttccccttca acagatagat cactgatctg cagtcctcga
     3901 tccgcaaaat tcgccccttc aacaaagtgg acattgctta aaatcgcttc atgattggca
     3961 agataattca agatccaaaa aacttctctt cggctaaaag atacatgatt gaccatccac
     4021 gttaaaaaat tcttcttttc ccgtacatca attagcattt ttctcacctc tctgttaggt
     4081 catctaataa tgacagtgcc tccatatctc ctggttcaag tgttaaataa taagtcaata
     4141 attcttgcgc tcttttcagc tgcccttctt ctcttaagaa aaaggcatac tctttcaaga
     4201 aatctggttc atgtgatagt tcatgataag cctgttcata gtgtacagct gcaacggtaa
     4261 agtcttctaa ttcattataa gcatgggcta ggttccattc tgcataagga ttagcagttt
     4321 cttccatctg attgatcact tgtatcacat cttcatatct ttcatcatcc aagtaaagat
     4381 tacttaaagt aagtaatgtt tcatcttgtt tgtctcctac ttctaaagct ttcagtaaaa
     4441 gttcttctgc tcgctgtcta tcatgcagac ggaaagcatt ttccgaggcc aggtgataaa
     4501 gttcgacttg gtaaggattt tctttgattc cttcttctaa cactgtttgt gcttcttcaa
     4561 tcatttcttc ttcttgaaga gcttgtccta gatatagata taacgattga taatgcggat
     4621 taacttctcg aagttcttcc agatagttga ttgcctgctg attttctttc aattgaagat
     4681 tcgtaaaagc tagctggaac agtcgatcat ctgtcctttc ttctttcagt gcttcttcta
     4741 aaggtacgat agctgcttcg aattctcctt gcatacttaa ggattgtcct aaacgttcct
     4801 gcatcgagat tcctgaaatt tcatttatcc cgcttgccaa caatgtttca taaaccactg
     4861 ctgcctcttt gaaacgatca actgaaaaat acaattcagc tagcgcaaac tggatcaatg
     4921 gttcatcagg aagcaaccca gctgcttctt tcaattttgc ttcactcact tcagggattc
     4981 cgataacttg gtacaaatca gctatcgcca ataagctttg cggatagtaa tcactttgct
     5041 tatccacttt ttccaggtat tcaaaagcta agtcgatctc attatcttcg attgcaattt
     5101 ctgctaacgg gatgttcagt ccgtcatttt ctgggtattg gttgatcagt tgttcaaaaa
     5161 tctgttttgc ttcgatcaaa aagccaatag ccagcaattc ttcgcctagt tcagctaata
     5221 tatctgctgg gtcttctttt aatgcttttt ctaaaaagat ttgtgcttgt gacaagtctt
     5281 catctgataa agcttgcaac attttttcgc tatttgtatt catttcaatt ccctcttatc
     5341 tcactgacga tttgttgaaa taccgtcatt gttctcaata attctttttc gtttggatgt
     5401 tggtatccgt ctattatacc tattttttct aaaacaagta tttttttgct tttttctgcc
     5461 aaaaaaagaa ctttttccaa atactccatc tttgaaatct gttccagcca gtggattgga
     5521 tagccaagca agtccagcca cacaaaaaag ttttttagtt ggaaatcaaa aggatatgct
     5581 tggagattcc acacgaaatg aaacatcatt cctaaaaatt ttttcatact agcagaaagt
     5641 agatggccat tttcggtcag ataaaaagct tgttcaaaca gtttaccata atttgacagg
     5701 ttttctgcct ctaattgata aaaatagatc atttcttcga tcatcccggc aaacggccga
     5761 gtcgttaatt gctttttatt tggatagttt ttatacagat tttgcaaaaa acgatgatca
     5821 caaatcattc cgcaacagat aaaagtcaaa aagtcaatct gctttccgtc cacctgccgc
     5881 tcgctgattg tttgatcata aacgatgcgt tctggcagat tgatggtttg cagcactaca
     5941 cgatttggta gctgcaaaat cgtccgctgc ggtgttaatg ctcggcttag agaacggatc
     6001 gatacaggga tcatccatag tgatgaatca agggttgtat gtttttgaaa aaaacctgcc
     6061 agatccatga tcccttcatt tccaaatccg atgataaaca tggattgatt ttcgggatag
     6121 cgtttgacga aatcaagcag gttctttaat tctgttaaat gattgcattg agtgttggca
     6181 caaatatacc agtcattgtt tgcttgattt gcaaataacg ggttgatctt atctgcaaac
     6241 aaatcataat agcgctgatt cgttaaaaat aagattggct gatcaatgga aagttctctt
     6301 agctgactga ctagcgtttc tccgtaaaac actttcgtca acgcatcttc tacttgataa
     6361 tcaagttcca tacgcatccc tcctcttctt atttttattt taccattagt aagcaaattc
     6421 tagaaaagat aagagcaaag ctcacctcat aacttggaaa taaataaaaa ttaaataaaa
     6481 tacatgtgtt ttctatgtat gacatacgga ttcggaatct ctgtctctta tttattcctt
     6541 ttctgctagg aaataaaaag aaaagcactt gtagccctag agccacaagc gctttttcaa
     6601 ctgtcttatt taacagcgtc ttttaatgct ttacctggtt tgaatgcagg tactttgcta
     6661 gctggaattt cgatttcttc accagtttgt gggttacgac ctttgcgagc tgcgcgttcg
     6721 cgaacttcaa agttaccaaa accgattaat tgaacttttt cgccgttagc taaagcatct
     6781 tgaatagttg aaaatacagc atctactgct gcagttgcat cttttttagt taaatcagta
     6841 gctgaagcaa ctttttcgat taattctgct ttgtttgcca tggatagatt cacctcctcc
     6901 acaagagata tgatagaaat atcatttagt taaaactttc tgagtggtcc agaagtttaa
     6961 aaagaaatat tgattaactt caatattctg ttatcacgat atcatatgaa ccgcttaata
     7021 gcaaggtttt taccaatttt cacatgcttt ttttagtcat agaaacgtaa caaacgaaac
     7081 tgtctttttc ttttgaaaaa aaaacgtcga tatggtaaaa tgcctatttt ctcctacgag
     7141 ggataattcg gatggacgtc ccttcaaaag taaatgcttt tctgatttga ttttctaaga
     7201 aacgttcata agaaaaatgc atcaattcct cttcattgac aaaaacgaca aatgtaggtg
     7261 gtttgacagc gacttgtgta gcataaaaaa tcttcaggcg ttttccttta tctgttggtg
     7321 ttggattgat tgctacagca tccataatga tatcattcaa cacagccgaa gagatacgaa
     7381 gattctggtt catacttact tgttcgatca tttcaggcag acgttccaac cgttgtttag
     7441 tcactgcaga aacaaaaacg attggagcgt aatcaagata ccggaattca tcacgaatct
     7501 cttgttcgaa atcacgcatt gtattggttt ctttctttac aagatcccat ttattcacga
     7561 cgataataat tccacgacca gcttcatggg cgtaacccgc tactcgcttg tcttgttccc
     7621 gaatcccttc ttcagcattc agtaccatca aaacgatatc agaacggtca atggcacgca
     7681 ttgcacgcat cacgctgtat ttttcagtat tttcgtatac ttttccgcgt ttacgcatac
     7741 cagctgtatc tatcattaag aatttctgac cactttcaga aacgaagtgg gtgtcgattg
     7801 cgtctcgggt cgttccttca atatccgaaa caattacacg ttcttctcct aaaatcgcat
     7861 tgatcaaaga ggatttacca acatttgggc gaccaatcaa actaaactta atcgtgtcgt
     7921 cgtcctcttc ttcagtctct tcagagaaat acttcactgc ttcatctaaa atatcaccaa
     7981 tccctagtcc atggcttcct gaaacaggat aaggatcgcc tagccctaag gaataaaatt
     8041 catagatatc gtttctcatt tctggattat ctactttatt gactgctaaa atcactggtt
     8101 tattacttct gtataaaatt ttcgcaacca tctcatctgc gtctgtcacg ccttcgcgtc
     8161 cgctgactac acaaatgatt acatctgctt cttcaatcgc gatttctgct tgatgcttga
     8221 tctgatccat aaaaggttca tcgccaagat caattccgcc tgtgtcgatc acgctgaatt
     8281 ctcgacctaa ccattcacct tttgcataaa tacgatcacg agttactcca ggagtatctt
     8341 cgacaatcga gatacgctca ccagcgatgc ggttaaaaat cgtagactta ccgacgttcg
     8401 ggcggccaac gattgcaatt gttggatttg ccatattatt tcctcctcac aagtttccaa
     8461 tctcacttag tttaacgaat gcattgccaa ctttcaagca tagattgtca attaaacgaa
     8521 gtatttttat tccttattga aatactaatc gcttttcaaa agaaaaactg agacaaaagc
     8581 agctgctttc atctcagtca cagatacaaa atcaattatt tttcagaatc atcggttgct
     8641 ttttcttcta cttcttcttt caacgcatca cctaaaatgt ctcccatagt aaatccagta
     8701 ttttcttctg gtaattcata agactcaaca ttttttggtt cttcttctga agctggtttt
     8761 tcttctaaag ctttgatact caatgcaata cgatgttctt ctggatggat ctctaatact
     8821 ttcactttca cttcgtcacc ttcatgaagc acttcatgtg gtgtcgcaat atgtttatgt
     8881 gaaatttgag agatatggac tagcccttct actccaggga acacttcaac aaaggcaccg
     8941 aagcttgtca aacgtttaac tgttccgtct aatactgtgc caactggagc ttttgtatcg
     9001 atttctgacc aaggtccagg ctgtgtgtct ttgattgaca aggaaacacg ttcttgttca
     9061 ggattgatag acaacacttt gacatttaca tcatcattga cagccaatac atcacttggt
     9121 tttgctacat gactatgtga aatctctgag acatggacta gaccgtcaat tccgcctagg
     9181 tcgacaaatg cgccaaaatc agtaagacgt gcaacacgtc cactgagaac atctccctca
     9241 tggatagttg aaagaatttc ttttttcttt tcttctcttt cgttttccac taccgcttta
     9301 tgcgataaga tcaaacgatt ttctgatggt tcgatttcga tgattttaaa gctcaatgtt
     9361 ttgcctttat agtctgaaaa gtctgcaaca aaatgatctt ccaccattga tgcagggaca
     9421 aaaccacgta caccgacgtc aacgactaaa ccgcctttga caacatttgt aacaggtgct
     9481 tcgatgacct tgccctcttg gaaatctctt tcgatatctt cccaaacttt tttagcatct
     9541 aaacggcgtt ttgaaagtag gtagctgccg ttttctttat ctttgccgat cgtactgata
     9601 acgactaaat ctaatacatc accgactttt actacttcgc tgatatcttc taccggcaaa
     9661 gttgataatt cttttgccgg aacgactcct tcaactccag ccccttcgat tccgacaatc
     9721 acttgtttgt cttcaatgac taaaacttcg cccttaacaa catcgccaac ttttacttct
     9781 tgtacgcttt tcattgcgtc ttccattgac tggttgttct cttgattttg atcaaattct
     9841 gtcataacac ttgtcctcct aaccaacatt ctgccgtaag tacggtactg tttttagttt
     9901 aattgattat tcaaaaaaaa taaagcgatt tcgttcgttt tccttgaaaa aacaattgtt
     9961 tttcattttt tttcttaatt taaaaaagaa gataaccttt tttcaaaaca acagctttta
    10021 ttgctgcaac gacttctgga atcgatttgc cagtcgtatc gatacgaaca gcatcctctg
    10081 cttgcttcag cggagagacg tcccttgttg aatcataatg gtctctttcc tgtatctctt
    10141 tcgtcagtgt ttcaagatca gtcggaattc ctttttcttg gttctctttg aaacgtcttt
    10201 ctgcacgctc acttacgctt gctactaaaa agattttcac ttctgcattc ggtaatacag
    10261 ctgtcccgat gtctcttccg tccatgacaa cgccaccgcg ttcccctatt tttcgttgga
    10321 cagcaactaa ttcttctctg acttttccat gtgcagagac ttcagaaaca gcttttgtga
    10381 catctggttg acggatttct aaagtcacat cttttgatgc tatgaataca tgctgtccat
    10441 cttcttgttg cgaaaaagag ataggatact ttttgatcag cgaaactagc ccttcttcat
    10501 ctgaaaaaga tacattgttc gtaatagcta aataagttac tgcgcgatac atggcaccgg
    10561 tatctgtata aatataattc aattcttttg ctaatatctt ggcaacggta cttttcccag
    10621 atgaagcagg cccgtcgatt gcaatactga tttgtgtcat gatataactc ctttttctca
    10681 gaaaaaaagc cgcaaagcgc ggctattatt atttaattct taattcttga ccagggtaaa
    10741 gcataaagtt gtttggatct aagccattca attcaaacaa ttgatcagtg gtgatccctg
    10801 cacgctcagc cacttgtttt ggaccttcgc ctgcttgaac agtcgttgtt gaaccggctg
    10861 cagctgatga agaaggtgtt gtttctgctg cattgctgct tgaatcccct gcttgataag
    10921 ttgcctcact tgaggaagtc ggtgttgtct ctgctgcaat actgctttct gacgttacac
    10981 ctgtatcttg agacacagta gattcttccg cgattgtact agattctact gttgaagtct
    11041 cagttgactc ctcttttgtt gtacttgtag aactttctac tactgatgtc gaagtttcag
    11101 aagaagttgc tgctgttgca ttgttgcttg atccatttct gatccagaaa taagcacctg
    11161 ctggaatagc gatacataag accagtaaaa tcaaaaatag cgaaagaaat aatgtatttc
    11221 ctttcttttg ttgtctctga gaacttcttg atgaaacttc atcatctgta tcgtagattg
    11281 gttgttccca tggttcttgt gtttcgttat tttctgtaga ttgatgtcta tcttttctgc
    11341 tcactgtttt acctcctaaa ctctatcgtg tgtattatac cataggaaaa agtgtcttgt
    11401 cctaaaaaat caatgttcca tgtttttttt aaataaattt agtaaaatcg tttcccaatg
    11461 aagttgttca gtcttagaaa aaagcttcct ctttggcaga agttctgctt ttgctccatc
    11521 aatatcacaa caattctgcg gcttttcgga gagtttttcg ccaaaataag ctaagatgaa
    11581 ttttctgcgg cagttttctt cattaatata gcgaagcatc tgctgcaggc ggaattcttt
    11641 ttctttttcc tgacgtttga gccgttccac ccaattttct ggtttttcag actgctggat
    11701 cagctccatc catttttttt gcagctcatc aaagggagct tgctttgtgg catattccaa
    11761 ataaagctca aaactttgtc gctgttctct agaaagttga ttaaagaaat aatggatacg
    11821 ttcatcccca ctttgataaa aaagaattgc tgcactttct tcctgatctc tgcctgctcg
    11881 accgatttct tgtacatagt tttctagact gtctggcaaa tcataatgga ccacataacg
    11941 gatatcagat ttatcgatcc ccataccaaa tgcattcgta gcaatgagaa actgcagctg
    12001 attttttaca aactgctgtt gaagttgtct tctttgagct gcatcaagac caccgtgata
    12061 gtatccaact gtaaaacgtt ccctgaataa atgatacaat cgttcgactt ctttttttgt
    12121 tgcacaataa atgattgccg ctccttgtgc tctttccatg aagtgttcaa gttcttgttc
    12181 tttttgctgc gttttccgaa caaaaagcga gatattttgc cgattgactg attgtctgat
    12241 ctcttttggt ggtcgatcaa atagtacatg acgtatatct cttgctacta aatctgttgc
    12301 tgtcgccgtt agtgccagtg tgaccgggtt tcctaaaagc tttcggattt ttcctaactg
    12361 ttgatattct ggacggaaat caactcccca ttgagacacg caatgagcct catctactac
    12421 atataacgca atattttgtt ttttcagatg ctctaaaaga gaaggattag tcaacatttc
    12481 gggactcaaa aacaaaaatt tatattgcga tagatgacgg aggacgtatc ttctttcaga
    12541 ttgcgaaagt aaactattga aagcagctac tcgtttctcc cctcgttttt gtaattgcgt
    12601 cacttgatct tccattagag aaagcagcgg tgacacgatg atgaccagac cttccattaa
    12661 ataccccgtc aattgatagc aaaggctttt tcccgttcct gtaggcaaaa tagcgagagt
    12721 atcttctcca tcaagcaacg cctgaataat ttcttcttgg ccgggacgaa atgaatcaaa
    12781 tccaaaatat ttttttaatt catttattaa cattctgtat cgcttccttt ctcaatagat
    12841 agatttgata caaacgaaat tctccataat ctaattgtcc atctacattc cattcgctaa
    12901 agcgccactc tcgcacggaa tccttatttg gttccaaacg ttcgattgtt tctgcagaga
    12961 gaatccactc gtatggaaaa tcatcaaaaa acaaggccca ctcaataaga tgatctgtta
    13021 ctgttcctct ttttaaatga cggattgcca tgatctggtc aattgtctga ccattcataa
    13081 acatctgctt ggtgatcatc atgctttgat tgaaattttg ctgtaataat gaatcgatca
    13141 aagcgtgcca aagcgaatct ggccgttttt cgatttgtgc taacaaaagg tggatacatc
    13201 tgctttgttg cagataaacc tctgactcat cattgttttt tgccagctga taaggaagca
    13261 gccctgttcg ctcatttcca gataattggt tagctaaaaa atctgctgct tcttctggta
    13321 tctccgaaaa cagctgcacc aaactctcat acgcagtatc aattaaaatt tcacgtggca
    13381 attggctttc actaagccat ttttttagtt ggaaagtata aaaaggactt gtctctgctg
    13441 gaagatagtc ttgaataccc gttgccagat tagaaattac ttgaacagca aatttaatga
    13501 gccgccaact agtttcccct gttctgccat agcggtcaaa acgcaaacca gtatgttcta
    13561 tttgatgttc ggacaacata tcagcccctg caggcgttag ctttgcttca ttttcattta
    13621 tttcgatcca accttgctgc gcaatttttt gcaaaatttg ataaaactta tcctgcttca
    13681 attctggaaa actgttatgg ataaacaaaa gttcgtgggt aaatccataa ataagtactg
    13741 aagtcgttcg ttttcccaca aggagttggt aaagagaact aatccgcagc ttgtgtcctg
    13801 atgaaaatag cgataaaata aaaacaatca tattattctc cttaagttag gatggttaga
    13861 aatgtcacta ctatgtaaac ttgtacctga acgctgtatt gcatgcggtc tttgtcaaat
    13921 cgaagcgcca gatattttcg attatgatga agaaggaatc gttttatttg cccaagaacc
    13981 agaggctcat caacagttcg tgatgccgga agaagaaaca tatgtaaaaa aagcgtatca
    14041 aaaatgtcca gttcgagcga ttttattaga aaataaaata taaatataaa cgaaacttta
    14101 aaaacgtaca ctaagttctc taaacttgaa aataagccgg gatctgtttc tcttgttccg
    14161 tgaaatcccg acttattttg aaagattcat ttgctatcta tagttaattg actagttttt
    14221 tcacggaccc tataggaccc atttatatta tttcaacttt ttcagccagt tttccaatgt
    14281 attgacgatc acagtttgct ggtctgcgtt agaaatttct gcctctccat ctcctttttg
    14341 ctcaccatag ctgccaaaac ctccatgatt tccgccattg attgatacat actctgttct
    14401 ttccggtaaa tactgcttag cttcttgata agcttcctga ttcaatactt tatcttttgt
    14461 cgccgtgatc gataaaacag gaacagctga ttctgtcaat gttccttttt tgtcgggata
    14521 acttgccatg aagaataccc cttttaaatg tttgtctgaa tgattcttat tgacataacg
    14581 acttgccatt acgccaccta atgaatggcc accgatcaca taatcggaat ttggaaaatc
    14641 ttctttgacc ttatccgctt tgtttccgcc catcactgct agatcaagtg gcattttgac
    14701 aatagcgact gaaaagccgt tttttgctaa atcttgcgcc caaatactat aactttctgg
    14761 ctctacgaga gcaccaggat aaaaaataag taatggagac tttccatgat tctttttctc
    14821 tccttcaaaa aataagtaat cttctttgtt ttttccttct tttaccaaca cttgcgcttc
    14881 tgacgatggt tcgtacgtat tttgctgtat atagtaataa ccggctccac cgagtaatcc
    14941 taaaatgatg actaagccaa ttagtattcg tctaaatatt ttcactttct cccacctttt
    15001 ctatctgagt gacccctttt tcttaatcaa gtatgattat accttaaaaa aataagagag
    15061 aaatgcaaac tttccagcat caaataaaaa aagacggaat atccatttga atatccgccc
    15121 tttttgtatg tttattttta tttagcacat aaacgataca cagcttctgc ataaatttcc
    15181 attgctttat acatactact taattcccat cgttcattcg cctggtgcat aaagtcagga
    15241 gtatcaggga acatcgcacc gaatgccaca cactggttca tcgtacgagc aaaagtagcg
    15301 ccgcctgata caaacggttc agtcatgtct ccagtaatat cgcgatatgt ccccaataga
    15361 tttttcacta actcactgtc tagtggcacg tataatgaat caaggaagtc aaattcttca
    15421 taagtcaacc catatttttc tgctgtttta gttaatttag ctactaaatc atctttcttg
    15481 aatgtaacag ggattctcat atctacaccg atttttgttt gttctggtgt gatttccaaa
    15541 cttgcaaagt tcatcgttag ctcgccggat tgttcatctt ctgttttgcc cacgacagct
    15601 tcaccagttg cattttcttg aactaatttt cctaagaaat tgattgggcc aaaatcaaat
    15661 acttctgaaa gagcaattgc cagacgagaa atagcattca ctccttgtgc tgcatctttc
    15721 gcgtggatac ttttaccaag aacgacgatg ccttcacctt gttcttcgaa atcgaattcg
    15781 tgttttttca atgcttcttt tacttcagaa agcttttctc cagagtaagg agctgcatca
    15841 ggaacaacat tcaacgctcc tccagctttt acagaaaact cgttcgttcc tggtcctgtc
    15901 aagtaagctt gcaataagcc tttttctgca taaattaaag gaaattcagc atctggcgca
    15961 aatccttgcg taattccttc ttctttttcg ttatattttt ctaaacaacg ccataaattt
    16021 tcttcatctg taccaaagat aaaacgaata cgtgtcttga attccacgcc agcatcaatc
    16081 aaagctttga cggcatacat tgccgcaata gaaggacctt tgtcatcttg ggatccgcga
    16141 ccaactaacc agccgtcttt tatagttgga tcgaatggtt ttgtttccca attattttcg
    16201 tcccctgctg gaacaacgtc catatgacac aagataccga ataattcatc tccagagcca
    16261 atctctgaat aaccgtaata gccttctgga tctttaaatg ttctaaatcc tagcttttca
    16321 ctgatttcca atactttatc caaagcttca atcacttttt taccgaaggg atgtccttgt
    16381 cctgaatctg cttcatctag tacggaagga attcggatca attcgctcaa tgattctaat
    16441 gcttgttcat gatgttgttc tgtcacaaat tttttcatga tattatcctc ctaatatgtt
    16501 aaaaagggta atgtcaaatt cctccgacac aacctcttct tctcaaataa tgacgtttta
    16561 agcctgctcc atcagcaagc agaatgttct taacaaatga tgaaagaaaa aacactcact
    16621 gaatggagct attgacttgt ccacgtgctt atcgatagcc gtttatcggt ttcttcattc
    16681 ttatgataga ccttgtacct gtatttgtct aggtttagaa cattgctgct acacctagga
    16741 tcacacagat ggctacaaat aatatcgcaa ttaatttacc ggtaaatttc caccacactg
    16801 aaacatcgaa acgagcaatt gctacagcac ccattacgat agcagatgtt ggtgtaatta
    16861 agtttactaa accagatgca gattgataag cagtgattac taaatcctta ccgacgccgg
    16921 caaattcacc cataggtccc atgattccca ttgttgcagc agctaagccg gaagttgaag
    16981 gaatcaagaa tgacataggg atatagaaaa taaatgtaat aatgatgaat acacttgaag
    17041 ataatgaact taatccttgt tcgccccaat gtaagatggt gtctgtgatt aatccattgt
    17101 tcataaccac ttggatacca cgagcaacag ctactacgat tgctacaccg atcaaatcag
    17161 aagcaccagc taaaaattca gagacaaatt cagactcttt catgccataa gtgattgcta
    17221 ctacgatgga cattactaag aataacatag taatttctgg gaagtaccag tcacctaatg
    17281 gaagcatgct tttacctaga accgtaccta gtaccggaag gtttgttaac cagtttgtca
    17341 atgattcaaa aatcgtgaaa ttcgcattca aggttgtcca aggaatcaaa ctgatgatca
    17401 tgataccaaa agtaataaag aacaatacat taacatgttt ttgttttttc gtgattactt
    17461 cttgacgacc gattgcttca atatcgaaat gcttcatatc ttctttacgt tggttgtata
    17521 ctaatgattt tgttggatct ttttcgatct ttgatgcata acgatacaca tatacaatag
    17581 aaatagctaa caaaataacg aacatcaaga atcgaagacc aataccttca cccatcgaaa
    17641 tcccaacagc ttgtgaagct acgcctgttg caaaaggatt taccgttgaa gctagacagc
    17701 ctacttgaga ccctactaat acgattgcta cagctactac tgtatcaaag cctacagcca
    17761 tcatgactgg gatcagtaaa gcatagaatg gaatcgtttc ttctgccatt ccgtaagtag
    17821 agccgcctaa tgcaaataac agcatcaata ctggaataag cattttttct tttcctttga
    17881 atttcttcac aactgaagca atcccggcat ctagtgcccc tgttttgttg ataataccta
    17941 aaaatccacc aacaaccaaa atgaagaaag agattgggat tgcagcaggt gtttctttag
    18001 ttagctcccc cgctggtaca cctagcatcc cattgattgg cgccatgaaa atatcccaca
    18061 gcccttgagg attttgttgt actcgttcat atgatccagc gacgatgttg cctgcatcgt
    18121 ctacactata gtgcccagct ggtataaacc aagtaagtac agcgattact gcaataataa
    18181 taaataagac cgtatacgct gaaggcattt gaaatttttt cttttttgtt tgttccatct
    18241 tcttcttcct ttcttttccg ttagagacaa caattttggc tctaatgtta ttttatcaaa
    18301 aagagtatca gaacaaaaat aataacacgc attttgtaaa acttttgttc aaaaagttcg
    18361 tttttcccta ttccctcctc ttttttcagg tttttaaagc tttcttcttt ttcattgtag
    18421 cgtttccttt tttttataaa caaacatttt cgtcttttaa aaggagaaga atttcacatt
    18481 tgcaaagaat tgattgattt ttatgcataa atcaaaaaaa tactcaaaac aaacaaataa
    18541 aacgtttaca ttattgcatg tcttgtattt ttttccaaaa aaaaagacca agaaatttct
    18601 tgatcttttt gatatttatt tgactgctgt atgacgttgt tcgaactgat gttgttttcg
    18661 acttaaccat ggaagaagtt gcgcatgcag cactaaaaag acagcactca cgatgaatcc
    18721 tttgatcaag ttgaaaggaa cgatcccaat cagtacatag tttgctaaag acatgcccag
    18781 catctgtgtt gcgttcagcc ctaaaaacat taagtaaaga ggcgtaataa cgaaataatt
    18841 tgcgatactc ataaaaatag ttagagcgat ggttcctgta atgacaccta aaaatttgtt
    18901 tcttccttta tgctgtttct ttacactaaa gaaataataa atcgggaatg taaagatcgt
    18961 tgttgataag aaactagcag tgtctcctac caaattatcc ggagaaaaac ctgtagtcag
    19021 cagatgtaat actgaacgca agaacgctgt tgcaactccg gccgctggac cgaataagaa
    19081 catgctgatc aataccgaga tatcactaaa atcgatcttc aaaaaactaa aagccggcat
    19141 gatcggaaaa gcaaaatact gtaaaactaa tgcgatcgca gcaaacatcg ctactgcaac
    19201 cattttctgt acacgggtgt tcttcatttg aaatcctcct gttaggactc ctcttcagcg
    19261 aactttttga cgtttctctc caaatgaaaa aactctattt tccagggaaa atagagttag
    19321 agttatttga tcaagcgtcg tcaaataagc tctatcttct ttatccagac tatactgtcg
    19381 gtatcggaat ttcaccgatt cagctatttt tttgcaaaat aggtcgtgga ctataaccac
    19441 cggtcgggaa tttcaccctg cccctgaaga cgaaccttat ataatttttg tttacatatt
    19501 cattatacat atctattcaa aaaacgcaac atctaacact tataaaaagt aagtgttcat
    19561 tttgagtggt tcagcaaact gctgacttct tttttgttca gttcacggta atccccagga
    19621 cgtaggcctt gtaaagtcag atctccgtat ttttcacgtt ttagtttttg cacaggaaga
    19681 ccaactgctt gaagcatatt tttcacttga tgattacgtc cttcgtggat cgtcaattcc
    19741 acgatactgt ggtttgtctt aggatccact gataaaattt ggaatttagc tggagatgtt
    19801 tttcgtcctt cgatctttat tccttttgtc aaaggtgcaa gcatcgattt tgtagcgatc
    19861 ccttttacct ttgcaacgta aactttatcc acttcatgtt ttggatgtgt caggcgctga
    19921 gcaaaatctc catcatttgt cagtagcaag atccccgaag tatcgtaatc cagtcgacct
    19981 acaggataga tacgttcttt taccatcggc aaaagatccg taacaacttt tcttcctttg
    20041 tcatctgcaa cagcagaaat aactccttta ggtttgtaca ataaatagta accgtattct
    20101 tcttgataaa ttgggacttc atccacttcc acagtgtcat gtttcccaac ttttatccct
    20161 aattccctta caacttgtcc gtttactttt acacgtcctg caaggatgaa ttcttctgct
    20221 tttcgacgag aagtgatgcc agcgtgggca attactttct gcaatctctc cattacaatt
    20281 attctccttt attcattcca tcaaagaata gatctggtga cacgtcttct tcaagtgaaa
    20341 tttccatttc ttgtgtatca ggcagttctt ctaatgattt caaaccaaaa taatctaaga
    20401 agtagccagt tgttccatat aatatcggcc gacctggtcc ttctactcta cctttttctt
    20461 caatcagttt atgggcagct agacgctgta ccgctcctga aacctgaact cctcgaatct
    20521 gctcgatttc agctcttgtg attggctgtt tataagcgat gattgctaag gtttcgacgg
    20581 cggcttgaga taatgtgttt gatgtccctt gcgcatatcg cttaagtaag ggagccagct
    20641 cttttttagt cgttaataca taatggcttt ccgtttcaaa aatatgcaaa gctgagtgcg
    20701 agttttgagt gtaagattct tttaattctt tcactaaact tgtgacttct gcttcgctga
    20761 gatttaacag tccgcttaat tcttcattac tgatcccttc ctcgccaaca ataaaaagaa
    20821 gggcttcaag ttcacttaat ttcgtcatgt tcggtatctc ctactgctgg ataaagcata
    20881 attgattcat agttttcttt ttgttgcgca atgatctgcc cttttttcat caattctagc
    20941 aaagccataa aagtagtgac aacttcactt ttagaaggtg actcaaaaaa agaatcgaaa
    21001 gtacagccat gcgcatttct ttttatacgc tcttcgataa ataccattcg ttcttcgatc
    21061 gttgtgtcat cgggcgtgat cgttttttcc actttttgag aaaatttctt acgttctaag
    21121 attttctgca tggcacggat caaatcgatt tttttgactt gtcctcgctt caacggcttt
    21181 tcagcatccg ctaacccatc gatgttcata ggttcttttg tgtaataaag acttcgttcc
    21241 tctgcttttt ctgtcagtct ttcagctgca tatttatatt tacggtattc taaaagctga
    21301 gtgaccaatt cttctcgagg atcttcttcc cataattctt catcgtctgt gccgaattct
    21361 tggacaggaa gcaatgtctt gcttttgatt gccatcaatg tagcagccat cactaagtat
    21421 tctcctgcaa cagctagctc gagtgttttc attgtatgga tataattcat atattgctcc
    21481 gtgatcgtag cgatcggtat atcatatata tcgatttcca tttgctgaat cagatgaagc
    21541 agcagatcga gaggaccctc gaatacatct aatttaatat tgatctccgt caactcattc
    21601 actctttctt ctttttcttt gctagccatt ttccaatgta aggctctgtg atttcagttg
    21661 cacggatgga gacatccggg tacttttcct gtaattcatc taacatttga aagcccatat
    21721 tctcaccgcg ataagacgga ttcaaagaaa tgtcatggat gatcagctga tcgttcgttg
    21781 cccagtaaat accaataatc ccttgaatgt tggttgaacc ttctggcgta aagaaaaaaa
    21841 gttcataatc gtcttcagat tcgtacgtat caatctcctt caacaaagaa gttttacctg
    21901 tcagtttctt atggaaactc aatagtccca tagcaatttt acggttttct ttccggtaat
    21961 gtgttaacat cttctcacct tcctatgact aagcccttgg aaaatgctgc ttataaacat
    22021 ccgtcattct tttcttcgtg atatgtgtat aaatctgagt cgtagagata tccgcgtgac
    22081 ctaacaattc ctgtactgtc cgcaaatcag ctccattttc tagcagatgt gtggcaaagc
    22141 tatgccgcag cgtatgtggt gttacttctt tgtaaatccc agcctctcgt actaactgct
    22201 tcagattttt ccagattcct tgccgagaaa gacctttccc atgattattg acaaatacat
    22261 gcatttcaga tgcgtccttt acaagtaatg gacgtgcttc gtctaagtag cgttccagcc
    22321 attggatcgc ataatcacct aaaggaatga tccgctcttt atctcctttt ccaactgtct
    22381 gtaccagtcc aagagaaaga tgaagatctc ccaatttcaa tccaatcaat tcactgacgc
    22441 gcatcccagt agcatacatg acttccaaga ttgcacgatc tcttatccct aacgtttttg
    22501 tcgtatcagg tgcttcaatc agcttttcta cttctgtcag agtcaatgta cttggtaatt
    22561 tctgtgcttt cttcggcgta tcgatatgtt gcatcggatc atgatcagtg atacgctctt
    22621 gccgtaaaaa ctgatggaat cttctgagac ttgagatcat ccgagtaaca gttgctgtcg
    22681 cttttttttc ttcatgcaac ttctccagaa aacggatgac taaaaagcga tcgacatcct
    22741 gccatgaagc aattttctgc tcctgtagaa aatgcaagta ctgttccaaa tcacgttcat
    22801 agctttgtcg tgtattggcg gacaatcccc gttcaatatt caagtagtgg agataatcgt
    22861 tgatttgctc tttcataata atccccctca tgcctctcta tcatatcaga taccgcttca
    22921 ttctgatagt ctttttccat aatctgagca aagaaaaagc cgtctagaca ctcagacgga
    22981 ctggttt
//
LOCUS       DACDIG010000044        22532 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.044, whole genome shotgun sequence.
ACCESSION   DACDIG010000044 DACDIG010000000
VERSION     DACDIG010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.044"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(101..1474)
                     /locus_tag="IV247_001765"
     CDS             complement(101..1474)
                     /locus_tag="IV247_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8777836.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(1474..1869)
                     /gene="rbsD"
                     /locus_tag="IV247_001766"
     CDS             complement(1474..1869)
                     /gene="rbsD"
                     /locus_tag="IV247_001766"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAP8777837.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(1897..2814)
                     /gene="rbsK"
                     /locus_tag="IV247_001767"
     CDS             complement(1897..2814)
                     /gene="rbsK"
                     /locus_tag="IV247_001767"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAP8777838.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(2922..3935)
                     /locus_tag="IV247_001768"
     CDS             complement(2922..3935)
                     /locus_tag="IV247_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8777839.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(4016..4615)
                     /locus_tag="IV247_001769"
     CDS             complement(4016..4615)
                     /locus_tag="IV247_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8777840.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            4801..5502
                     /locus_tag="IV247_001770"
     CDS             4801..5502
                     /locus_tag="IV247_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8777841.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            5512..8223
                     /locus_tag="IV247_001771"
     CDS             5512..8223
                     /locus_tag="IV247_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP8777842.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGTIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            8674..10302
                     /gene="treC"
                     /locus_tag="IV247_001772"
     CDS             8674..10302
                     /gene="treC"
                     /locus_tag="IV247_001772"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAP8777843.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(10362..11543)
                     /locus_tag="IV247_001773"
     CDS             complement(10362..11543)
                     /locus_tag="IV247_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8777844.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            11795..13318
                     /locus_tag="IV247_001774"
     CDS             11795..13318
                     /locus_tag="IV247_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP8777845.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(13387..14115)
                     /locus_tag="IV247_001775"
     CDS             complement(13387..14115)
                     /locus_tag="IV247_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8777846.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(14128..15408)
                     /locus_tag="IV247_001776"
     CDS             complement(14128..15408)
                     /locus_tag="IV247_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP8777847.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(15549..16151)
                     /locus_tag="IV247_001777"
     CDS             complement(15549..16151)
                     /locus_tag="IV247_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAP8777848.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            16396..16602
                     /locus_tag="IV247_001778"
     CDS             16396..16602
                     /locus_tag="IV247_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777849.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            16914..17633
                     /locus_tag="IV247_001779"
     CDS             16914..17633
                     /locus_tag="IV247_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP8777850.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(17714..18397)
                     /locus_tag="IV247_001780"
     CDS             complement(17714..18397)
                     /locus_tag="IV247_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAP8777851.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            18615..20801
                     /locus_tag="IV247_001781"
     CDS             18615..20801
                     /locus_tag="IV247_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP8777852.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            21147..21557
                     /locus_tag="IV247_001782"
     CDS             21147..21557
                     /locus_tag="IV247_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP8777853.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            21571..21927
                     /locus_tag="IV247_001783"
     CDS             21571..21927
                     /locus_tag="IV247_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAP8777854.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT         7751 a         3973 c         4361 g         6447 t
ORIGIN      
        1 agtttggaaa gtatcacaaa aatttataaa aataatgatc ctaaaaagaa aaagactgaa
       61 atatgtgata aacatctcat acttcagtct ttttcattaa ctattcataa gacattaggt
      121 cttcatcaaa cccgctaacg agagtagtga tcattgctac gactagacta acagccagtc
      181 ctgctaagta ccagaaaaaa gtaccggaaa tgaatgcagg caatgtggtc agactaccaa
      241 atacaaaagc gatcgctttc acttgggcaa atcccattac agctccacca gcagctgcac
      301 caatgcaagc agcaacaaat ggttttttca atgggatcgc aattccgtaa acagttggtt
      361 cggtcactcc cgcaagtaaa atcgaagcaa aagcagaacc ggagatcgaa cgggtttttg
      421 aacgtttact tcgcagaaat acacctaaag ctgcaccagc cattccaaaa ttggctgctc
      481 ctgtcaacgg aacgattcgg tcgaacccat aagcagagat attactgatc atgacagggt
      541 ttacagccca ttggatacca aatattacca aaatattcca accaccagcg atgattgcgc
      601 cactgatcgg tccgttaata tccatcatcc aagtaattgc ggcagctaaa ccttcgccaa
      661 ggtaaacacc cgcaggcccg aataccatta acgtcaaagg aaccgtgata actaatgaaa
      721 gtaaaggtac aaatactaat tgcatatttt cgcgaatata ttttttgagg aatttttcaa
      781 caaaagaata aaagaaaata cctaaaatcg ccggcatgac agtagacgta tactgcatca
      841 gtactacagg aatccccaga aaatcagtag ctgctttgcc actgtctgaa agtaaagagg
      901 tcagattcgg ttcaatcagt gctgcagcaa tgacagccgc aatatatcca ttcactttaa
      961 acgttttggc agcagaaaaa gcaagaaaga ttggcagaaa ataaaagacg gcattcgacg
     1021 cagcagacaa aatatgataa gttccgcttg tttcactaag ccagccaatc gtagtcgcta
     1081 aaacaaccag accttttaat ataccagaac cggcaaacag cggaatgatc ggcgtaaaga
     1141 tttccgatat caaaccaaat agtttttcga tcggatttag cttttctttc ggagctgaat
     1201 gatcgatttg acgcgtcaat tccttgtaag caggagccaa tccctgatcc atgattattt
     1261 ctgcatatct atcgagtaaa atgaaatact taatggaagg gatttgctgt tttaattttt
     1321 ctgcattggc tactggctgt tttttcaatt tgatacgaag ccgagtcgca catggaagga
     1381 cagaatcaat attgtctttt cctcccaact gttcaatcag catatgaatc tgctcggaca
     1441 gtaccatctg ttcaggattc attgtcgttt ccattagaag accactccac tcactaatat
     1501 aatattggcg taaggtgtca tttcgcctgt acgaataaat gcatgacagt tattcaactc
     1561 ttttttcatt tcttcatgtg ggataaagct aatcggtgta tctggtaaca ataatttgat
     1621 tttttccaat atttccgggt tcattgtctt tatttcttcc gccaattcaa tcgattgtac
     1681 ctcaagttct tctaaaacgt tttctaaaac agataaaaaa cttggaagac cgtttgagac
     1741 agccaaatct attttttcag taccaaaggg gacaggcatg cctgcatccc cgatacttaa
     1801 tttatcaaag tgccccattt gtgcgattac tcgtgaaata tctgaattga ttacttttcc
     1861 ttttttcatg tttgtttctt cctctcattg ttaattttag actgctgatt gttctaaagc
     1921 ttcgcggtaa gggatagaag gttgcgcacc ataagactgc acagcaacag aagaggctag
     1981 atttccataa aggatcgctt ctttcaagtt actaaggtct ggctgaagtg tgctgcttaa
     2041 tgcgccaata aatgtatcac ctgcggctgt tgtgtcaacg gcatccactt taaaagcagg
     2101 gacgataccg tgttcttttt ctgtatgata gaaagcgccg gcactgccta gtgtaatgat
     2161 tactgttcca atacctaatt catggagttt ttcggctgct gcgaccaaac tgttatgatc
     2221 tgtcactctg attcctgtaa tgatttctgt ttctgtttca tttggtataa tgatatctgt
     2281 cttttcaagt agttcagtag gaatcgtttc acgagctggt gcaggattca agatcgttgt
     2341 ttttcctgcg tcttttgcaa tagaaaaagc taggatcgtc gcatctaagc tactttcaaa
     2401 ctgagcaata ataaagtcac tgtttgtaat cagttgcttg ttttttagga cttcttgttc
     2461 atggaaagca ttatttgccc ctgcatggat caagatacta ttttctcctg ctgcgtctac
     2521 cataatcatt gcttgtccgg ttgtggcgcc ttggatttca gctatcccag atgtatcgat
     2581 gttttctttt gtcagtagat ctaataactg ttgtccttcg ctgtcagccc caacgcctcc
     2641 aataaaagat gtccgtgcgc ctgaacgttt ggctgccact gcttggttgg cacctttacc
     2701 tccgccagcg tgaaaaatct catgcgcatg catcgtttcg cctggcttgg gcatgttggt
     2761 taaacgaagg gtagtgtcca tattaatact tccaattact gtaaccgtgt tcattttaca
     2821 aacctccaac tatctcattt attttgatgg gaaaattatt ttttaaaacg ttttagtaaa
     2881 acgttttaat agttcgataa aaatatagca caccttgaaa gctatttcaa gggtgcagtt
     2941 gaaaatcttt tttctaatcg tacaggtaat cttttttctt cccattcttt ttctgggaat
     3001 tggatccgat ctaacaataa cttggcagat gtttgtccca attcgaagat tggttgtgcg
     3061 atcgttgtca gtttcggctt gatatactcg cacatatcta tattatcata gccaataatg
     3121 ctgtagtctt cagggatgct ttttccagct tcctctaatc cgcgatataa tccaaaagct
     3181 aattcatcat ttaaagcaaa cacaccagtt gcagcagaat caagaagttc tgctgtgatc
     3241 tggtagccac cttgttttga gaactgtgta ggtaagagga tcagttggtc gtgagggatt
     3301 tggtaaacat caagagcaga tttaaacccc tcaatccgag cgtggacatt ttccggtgga
     3361 ttttcaggat agacaagggc gatcgtttga tgtcctagac taagcaagtg catgcccgct
     3421 aggtaaccac cgcgaaaatc gtctgttcgt acggcatcac taaatccttc agattttttc
     3481 tgatccaaaa caatgaaggg ccggccttgt tttttcaaat tttcgttgat tgcatctgta
     3541 gagacagcag aagtcgcgat aatgaatcca tccacacctc gacgtgtgag ctcggctaga
     3601 tattcgattt ctttttgatg atcagaatca gcattacata atatcgtgac gaaattctgc
     3661 ttatacaaga tatcctcaat tcctcgcatc agtgtactga agaatggatt cgtgatatca
     3721 ggaacgagta caccaattgt tttcgtttcc ttggtaatca tttgtctagc aaaatagtca
     3781 ggctgatagc ctaattcttc tttggcagct aaaactttct gaacagtttt cggactgaat
     3841 ttctccgtat ttccatttaa tatcaaggag acagttgtga ttgaaacgcc ggaaagttta
     3901 gcgacttctt tgattgtgat cttttttttc accatgttca tactcctatc taaatcctgt
     3961 ggttattttc ccatgaaatc aaaaagaagt aaatgaaaat cacgtgtgcc agtacttatt
     4021 tgctttgttg gatagcttcg attatttgtt tcgttgagcg tactcgtgca gttttcggaa
     4081 atatcttttt tatgaacaat tgatgggatt ctttgtcacg atcactgcaa gcatcttcag
     4141 caagcacgat atggtacccg tgttgaaagg catctaatgc agtagcatat acaccgtttg
     4201 ttgtcgctac accgcttaaa atgatagttt cgattccacg ccggcgaagt tgaagatcaa
     4261 gagaagtacc aaaaaaagca cttggattgt attttgtgat agtcaacata ttatctgtat
     4321 ctttcaaaga atccttcaaa agtaaatcag tgaattctgg aggtacagaa gcagtcattc
     4381 cacccatatc agtttgttga ctcaaataag ggaaagtgga gatgtctacg ttcactaaaa
     4441 ctttcaaggc agcggtacgt ttcaatgttc ttactaacaa atcattgttt gtcagaatag
     4501 atgctgcagt gtgtggagag agtgtaccgt tatttacgat ctctttttgc atgtcgatcg
     4561 cgacaaaagc agtttttgat aaatcaaaaa tcatattttc ctttaaatca ttcatgatgt
     4621 tccttctttc aaataacaat gttttttatg taagtaaatc ataaatcgtt tatttgtcaa
     4681 ctaaattaaa ggattccttt ctttagtcta gagaaattca gtggtaaaat aaataagtgt
     4741 gagaaaacgc attcgatgca tagatatagc gagaaatatc gtgaaaatgg aggaatcaga
     4801 gtgaagtatg tcgaagtaaa aaatgaatac aaacgataca aaatgggaga aacgaccatt
     4861 acagcaaatg acgatatttc tttttcaatt gaaaaaggcg aattagtgat tatacttggt
     4921 ccaagtggag caggaaagtc aacgattctt aatattttag gtgggatgga tacacctgat
     4981 gaaggtcaga tcattattga tgacacagat attgcgcaat tttcagataa acagcttact
     5041 gcttaccgac gaacagatgt agggttcgtt ttccaattct acaatctcgt tcctaatcta
     5101 acagcaaaag aaaatgtgga attggcaacg gaagtttctc ctgatgcgct tgatcctgta
     5161 gaggtattga ggcaagtggg cctagctcat cgattgaata actttccgtc ccaattatca
     5221 ggaggcgaac agcagagggt ttcaattgct cgtgcactag ctaaaaatcc caaacttctt
     5281 ttatgtgatg aaccgacagg cgcgctggat tttgaaacgg gcaagcaagt attgaaatta
     5341 ttgcaaaacg ctagcagaca acacggcaat accgttttga tcattacaca taattctgct
     5401 ttagcgccca tcgctgatag agtcattcac attaatgatg ctaaagtaag gtctgttgaa
     5461 ttaaatgatc atccttcatc cattgatgag attgtatggt agggggattt tatgaaaaca
     5521 agaacatatc ttaaagcagg ttttcgcgaa attcggcaat ctaaaggacg attcattgca
     5581 atcatcctta ttgtcatgct aggaacactt ttatttgtag gtgtcaaaac tgctggacct
     5641 gtcatgcaaa aaacgatgga tcactatgtt ggtacagcag gactttctga tttgcaaatc
     5701 gtttctactg gtggcttgac acaaaaagat attgctcaag cagaaaagat cccagatgca
     5761 aaagtagaaa caggaaaaca gatctattat tccaatactg gcaaaaacga agtgattcaa
     5821 atattttctt ataacaaaaa taacaagcag aacaagctac aattaactga tggaaaactc
     5881 ccggaaaaac caaaccagct tgtattggat gaaaaagcgg aagatgaagg gtataaaatc
     5941 ggtgatattt atcagattga ttcagatgaa ttgaaagaaa aagagtatac catcacgggc
     6001 tttgtccgtt ctccattgtt catcaacaat ttggagagag gatacgcgaa cgtcggaaat
     6061 ggtagcgtcg attattttgt ctatttacct gaatccgttt ttaaaacaga tatttattct
     6121 gtcttatatc ttgattttac aaacgtaaag aatttgaata cgtacagcaa agcttacaaa
     6181 gataaaatgg agcaaaatca agaaaaggca gaaaagtatt tacaagatcg acccgaagaa
     6241 cgcttagaag agctgaaaaa atcggccaac gaagaattag aaccggcaca gcaaaaaata
     6301 gcggatggca aagcacaaac aacgcaagca agaacacggt tagaagatgc taaaaaacag
     6361 ttagcgcaac agtcagcaac tatccagcag cttcctgaag cgcaacgaac aatcgctgtt
     6421 caaacattat ctcaacaaga agctcaactt gctgaacaag aaaggcaatt agttgagaaa
     6481 gaacgtgaac tggcctctgc agaggaagaa ttgaatcgaa atgcagaaaa aattgaaaat
     6541 ttaactaaac catcatacct atacaatgaa cgttcagaaa atgtcggttt tcaagaattt
     6601 ggtgatctgt ctgatcgtat cgctgcgatt gccaatgttt ttcctgtttt ctttttcttt
     6661 attgccgcat tgatcacctt tacgacaatg acacgtatgg tagaagaaaa ccgcagggaa
     6721 atcgggacat taaaggcgtt aggttatacg aaagttgaaa tcgcaaaaaa atatgcaatc
     6781 tatgcaagtc tagcctcagg gataggtatt gttttaggaa cgatattagg aacgaatctt
     6841 ttgccaagga tcatttttga gctgtctaac gagcggtatg atattgggag tgcgcttatt
     6901 ttttatgact ggccgccgat tttacaagca gctgccgcat tcttcattgc cgcatttggc
     6961 gctgcaatga tcgttttatt caaagattta agagaacggc ctgctgcatt gcttcaaccg
     7021 aaagcaccga agccagggaa acgcattttt cttgaataca tcactccgct gtggtcacgc
     7081 cttagcttta atcaaaaaat cagttaccga aatctcttcc gatataagtc aagaatgctt
     7141 atggcgatta tcggaattgc tggatgtgcc gggctgatgg ttgcaggcgt gggattgaaa
     7201 gattctctaa gttctgtttc agccaaacaa tttggaacga tcattgacta tcaagcaatc
     7261 gttacattgg attcatccac ggaagatagt cgtcaaaaag cagcggatat gctgcagaag
     7321 caagaaaaag taaatgagat cctagctatt catactcaaa ccgtagaatt gagaaaaaaa
     7381 ggacatgctt cgcaaagtct gacagtgatc gtaccaagta aaacagctga tttggatcgt
     7441 tttatccatt taactgatct tgaagagaac ccacgctcct tgcctaaaac aggaatcgct
     7501 attacgcaaa aagtagcaga actatttgac ttatctacag gagatcatat ttcgctttat
     7561 gatgaagatc aaaaagaatg gaaagtcgaa gtaaaagaaa tcgtgaaaaa ctatctaggt
     7621 agctttattt atatgtcccc tgaatattat gcttcagtta ctgatcaatc gatgatggat
     7681 aatgcatttt tgctgcgtac agataagatg acaacaaaag aagaagaaat gctttccgag
     7741 aaactgcttg ccacagacgc tgtgacaaat acgtctttca tgtccaaaca aatcgaaacg
     7801 caagaagaat caatggcgaa cttagactcg gttgtcttaa tctttgtcgt attatcagga
     7861 ctgctggctt ttatcgtctt gtataatcta actaatatca atatttccga gcgtgtacgc
     7921 gaattatcta caataaaagt ccttggattt tttgataaag aagtcacgat gtacattgtt
     7981 cgagaaaata tcatctttac tcttttagga atcatcggag gatttggtgt aggatatata
     8041 ttgactgact tcatcttgca acaagcgtct atggagaatg tcatctttcc attagtgatc
     8101 acatggtggg cttatgtctt atcagcagga ttaactatcg tatttacggt tattgtgatg
     8161 attgttaccc atttcaaatt aaagcatatc gatatgattg atgcgttgaa atcgaatgaa
     8221 taaaaacaga ggttgtgaaa tcagcggttt gacctgagta ttagagaaac aaacggcaaa
     8281 atagtttttc atattttgcc gttttttgag ctaataccga aggacagctg attgaacacc
     8341 atgaatcaga ggttgtaaaa aagccgtcct gcatcgagca aaaagaatac tcaaataaaa
     8401 ttagctcctc atgttttaca tttgagaaga ctgattgccg tagataaaag cttttgaaca
     8461 ccatttatag aagaaacaag aatattttgc tattttaaac aagaggatag acaaatacat
     8521 gtctgatctc ttgtttttta ttgggcaaca gacctcttag aatgtataaa gataaatttt
     8581 acaaacgctt tcggttcagt tataataata aatgataact tgtatatata agttgtggta
     8641 ggtaataaat cagttgaatg gaggtaaata tccatgacat ttaaagacaa agtcatttac
     8701 caaatctatc ccaaatctta caaagacagt aatggtgatg gaattggaga tttaaaagga
     8761 atcaaagaaa aactcccgta tctgaatgat ctaggcatcg acatgatttg gctaaaccct
     8821 atttatccaa gccctcaaaa agacaacggt tatgatatct ctgattacac agccatcgat
     8881 cctatctttg gtacgatgga ggaattcgaa cagctaatag ccgaagcaaa gaaataccgg
     8941 atagaaatca tgctcgatat ggtgctgaat cacgtttcga cagaacatga atggttcaaa
     9001 aaagcactag caggggacca gcattatcag gacttcttca ttttaagaga agagccaacg
     9061 gattgggtat caaaatttgg tggaagtgct tggtcgccat ttggagatac ggggaaatat
     9121 tatttgcact tatatgatcg gacacaagct gacttgaact ggcggaacca agcagtaaga
     9181 gaggaactat tcaaagttgt tcgtttttgg atggaaaaag gggtaaaagg attccgtttc
     9241 gatgttatca atgttatcgg taaagacgaa caattgaaaa ataataaaga aaatgatggc
     9301 aaacctgaat atacggacaa accaatcacc catacgtatc taaaagaatt gaaccaagca
     9361 acgtttggga cggattccac tatcatcaca gtcggtgaaa tgagctctac gacgatcgaa
     9421 aattgtatct tgtatacaga acctagtaga gaagaattat caatggtctt taatttccat
     9481 catttgaaag tcgattatga aaatgggaat aaatggtcta ctcctccatt tgattttgca
     9541 gaattaaagc gactatttca tacatgggga gaaaagatga gtgagggtaa cggctggaat
     9601 gctctatttt ttaataatca tgatcagcca cgtgcgctca atcgatttgt agctatcgat
     9661 cagtatcgaa aacaaggagc agagatgcta gcaaccatga tccatttgaa ccgtgggacg
     9721 ccctatattt atatgggaga agaaatcggc atgatcgacc cagactattc atcgatttcc
     9781 gactatattg acgtagaaag tttgaatgct tataagttac tccttgaaca aggattcaca
     9841 gaaaaagaag cgtttcgtcg gatcaaagca aaatctcgcg ataactctcg aacacccatg
     9901 caatggtcag acacagaaca agcaggattt actacaggga aaccttggtt aaaagttgga
     9961 ggcaagctag aagagattaa tgtagaaaaa gaactatctt ctgaagattc tattttgtcc
    10021 tattacaaga aattgattcg gctgagaaaa acatatccaa tcgttgcaca aggagattat
    10081 catgcttatg aagcaaatca tccggaagtt tatggctttc ttcgccaatt tgaaggacag
    10141 cagctgcttg tattaacaaa tttctatgat aaagatacaa ggatcgccat cccagaagga
    10201 ttcacagaag ccaatattct tatcgataat tattccgata ctgttcttca tcaaagtctg
    10261 gagctaaaac cataccaggc aattgctttg ctgaaaaatt aaaacaaaaa cacaaaacgt
    10321 cgttcctacg agctattctc gtaagatcga cgttttgtgt tttatttaaa ataatcttct
    10381 tcagaaaatg aagagttttc atactctcct ttatcgccat aaaatttatt gtagcgttgt
    10441 ttgaatttac ggaaaataaa agtaacgagt aatttgacta acgtatagat aggaatacca
    10501 aaaataacgc ccacaattcc cattagatca cccatgacaa gtaaaacgat taagatcgta
    10561 atcggatgga tctgtaaacg atcacccatt actcgaggaa tcaccaagtc accgtggaga
    10621 agttgtacgg caaaccagac aatgacaaat ttgacagcca taaaagtaga gtcttggaat
    10681 gcaatgatca aacctggaaa gaaggcgata aacggcccaa tatatggaat gatacataac
    10741 aacccagcag aaatagctaa aacactagca aattctaatc cgatcagtaa gaaacaaacc
    10801 cagtagactg ctccaaggat aaatgaagca atgatttgcc cctttagaaa tgaacccaat
    10861 tgttgattag caatctttgt caaattttta aaatctgtac gaaatgctgg tgggacaatc
    10921 gctaaaactg actgataaaa tttttgagga tctctcaaaa ggaaaaaagc gacaattgga
    10981 ccagtaaata aagtaataaa aatagctgta acagttgaaa aaatgttgct gatccgttgt
    11041 gcgccacttt cccaataatt tccaataaat ccagctatat cagacgtcac gtcgtcaata
    11101 gaagcgaaaa attgattaaa tgaatcggca aaaggtgttt tctccaaaaa ttgtcggaga
    11161 ctctcttgaa aatcttgaaa caaatctggc aaactactga tcaatgtctt tgcctgttct
    11221 tcaagaaaag gaataagcca tatgcttcca agccccaata aaaataaaat aatcacataa
    11281 acgataatga ctgacaagtt cctcgaaagg tgcttgctca aacgtttgat gactggttcc
    11341 aaaagataat acaagatcag tgcaaacaat aatgggggca agatagtagc gataatgatt
    11401 ccgacaggct tgaatataaa cgagatctga tggaaaaaat aaatgatcag cgcaagtaag
    11461 ataactattc ctaaaacata gtaagaggtt tttcctccta aaaagcggat caaacgattc
    11521 gttgtatctg tttctttttt cactcttttt cctcctttat ctataaattc attatagaca
    11581 aaagcgggta taaaaggaaa atgatagagg ttgattgcaa aaatatcgtc aatcattcat
    11641 ataaactttc tcatcaatta aaataagaaa acgcttgaca gttcttttaa ccatatgata
    11701 aattaaatcc tgttgctaaa acgtaactat tacggtttgc gaaaagcgaa tcggaacatt
    11761 caaatgaaag aagatgagaa gaggagtgtc ataaatgaaa cgattgatag gtatattggc
    11821 catgctggtt ttagcaggta tgttaactgc ttgcggggca agtgggaaag ccgaggacag
    11881 taaagaaaaa ttgagcgtga tgacgacctt ttatcccatg tatgatttta caaaagcaat
    11941 cgtcggggat gaaggagaag tagagttatt gattccagca gggactgatt cccacgatta
    12001 tgaaccttct gctaaagaca tggcaaagat ccaagataca gatatctttg tttataatga
    12061 tgaaaacatg gaaacatggg tccccgcaat ccaaaaaaca ttacaagaag gcaatgtcca
    12121 tacgataaaa gcgacagaag gtatgctttt acttccggga agtgaagaag gacatgatca
    12181 tgaccatgaa cacggggaag aaggccatac acatgagcta gacccacatg tatggctagc
    12241 tccaagttta gcaatcaagc aggtagcaaa catacgtgat cagctgattg aagcttaccc
    12301 agaaaaacag gaagtatgga caaaaaatgc agcagcctat accgagaaac ttcaagcact
    12361 ccatcagctt tatcaagaaa cctttaagca agcaaaacaa agatcttttg tcacgcaaca
    12421 tgctgcattc aactatctag cgttagaata tggattgaat caagtatcga ttgctggtct
    12481 ttcttctagt gaagaaccgt ctgcggcgag aattgctgaa ctgaaacact ttgttaaaga
    12541 acatggaatc aactatattt attttgaaga aaacgccaaa gatagtattg ccagaacgtt
    12601 agcaaacgaa gcaggtgtat ctttagaagt attgaatcct ttggaaggat tgacgaatga
    12661 acaaatagaa aatggagaaa actatctttc tatcatggaa gcaaacctcg aagcgttgaa
    12721 aaaaacgaca gagacagaaa acccgctaga agatactttg acccccaaaa aagaaaagag
    12781 cgtctacaac ggttattttg aagacgagga tgtacaagat cgctctcttt cagattgggc
    12841 aggaacttgg caaacagtag atacgtatgt agaagatggg acttttgatc ctgtttttga
    12901 atataaagaa aaattgaatc aggataagac agcacaagaa tataaagaat attatacgaa
    12961 gggctatcaa acagatattg cttcaatcaa gatcagtgag aaagagatgg catttacgtt
    13021 taagaatgga acaacgaaac gttcaaatta tcgttatgta gggaaagaaa tcttgaccta
    13081 cgaggcagga aaccgtggcg taaggtatct attcgaagca gaagataagg aaagtggcgc
    13141 ttttagatat attcaattca gtgatcacac gattgcaccg gctgactccg aacattttca
    13201 tttatatgca ggaaatgaaa gtcaagaagt attgctgaaa gaaatggaga attggccaac
    13261 attttatcca gatacaatgg atggacgtga aattgcacag gaaatgcttt tgcactgata
    13321 ggcaaaaaag aaccgaaggg tttggatcgg ctggtcattt agtcggtcca aactattcgg
    13381 ttctttttat gaaatcaatt tctttaatcc acggatagag gctgggatgg tctcgccgtt
    13441 tgccaacgtg acttctctgg tttttttatt aatcgaccgc acattttgtg tattgataac
    13501 ataggattta tgacaacgga taaaattccg atgcattttc tcgatttctt tgatcttctc
    13561 atagaattgt aattgggcat gagtcaaatg gacttccagt ttatgtggcg ctcctagcga
    13621 agtagaaaaa tattggatgt ctttgatcgg taaaaatttg atctcatcat taaactctat
    13681 tttgaagata tctttttgga tttgggacat ttgaaaatct tcgaaaattt tgctcaggat
    13741 atgctcgaca ttttctttta aatcataaag atcttctttg acgatataat cagtcggttc
    13801 gatgttgctt tttaagattt tcatcgacat ttccgtatgg gaagtcacat agattatttt
    13861 tcctaaagga tctaattcac ggatcaattt accgatcgct acaccatcca tttcactgtc
    13921 ttctagttcg atatctaaaa agtaaatacc aaaagaagaa gttctgatct tggctgcatt
    13981 gagaagttct tccggatcag aggtagaaat atggacataa ctgtctaagt tttcaatcat
    14041 gattctattt ttgatcaccg ttgataaaac ttctaattgt tttggatcgt cttcacaaat
    14101 ataaatggga atcatatcat tactccttta ttttatttgt atataaatcg tttgagaaaa
    14161 acgctggttt tcaattgatg tttcaaggaa aagataagga ttggtctttg tcaattcatc
    14221 caagataaac aagccaaggc cttcgttttt tggatttgtt tttgttgaga accctttttg
    14281 tttcaactgg tttaacggta atgtatttgt tgttgtattg atcacacgaa tcatcacgga
    14341 atgattcttc ttgagtatgg tcaattcgat ttttttcttc tttgcagcac tagctccttc
    14401 aatcgcattg tccaagataa taccaagcat acgtacaagg tttaccgtat gtttcgtatc
    14461 cagttcgatt ggctcaggaa aatgaagctg gacgatcaac cctttttcct gtgccatcat
    14521 gagttttaat gacagtaaac tacggatctc aggaactttc atattatcca acatacttag
    14581 attggccgga aaaaggttca tcatctggcg tgtaggtaat atcgtttgat cataataaga
    14641 ccgtaatcct tctaagtcgc cttctgaaat atagctgtga agagaaaaaa gcagattttg
    14701 gtaatcatgt ttgaattctc tcaagtcttg atagtttttt tctagatctt tgatataatc
    14761 ctgttgcatc ctgacttgtt gctctaataa ctgatcagct aatttcttat tagagatttt
    14821 ccaataataa accaagatga tcaggccaac acacagatat agaatgaaaa aactcgtatt
    14881 caaagctaag acatcaggtg tttcaccaga aaaacgggta taaaaaataa tgatatagta
    14941 tgtaaataat atcaatgtcc cgatcgtacc gatcatacgg cggtccacag tgtgaaacca
    15001 agtctctaac gattttttta aacaaaaagc taataaagca gataaaaaag cggataagac
    15061 aatgtgaaaa tactgcaatg aatctcctgg ttcaataacc gaagatttca aaacaaaaaa
    15121 atcgccgatg cttgttaagt gatctcctaa aagcgacgtc aataacccaa aaggaagcgc
    15181 gtaaaccaat tgttgacgcg aagtcgaaaa gaagaaaaaa gtaagcatca gcattcctat
    15241 agcaattaga gcagtaaaaa tccctaaaat cgaatagacc catccaatca aaatacaaga
    15301 aatcagaatg actcccgcaa acagatattt gtatcggatg tgatggtcaa tcaataagat
    15361 acaaattgtg taaatcaaat attgcaacgc tatggaccat aaaaacataa agtacagctc
    15421 ccattgattt ttaccaaatt atagcacaat tggcgtatta tttttttaat ttttcaggga
    15481 ttttgaattc agaactcata ccaaagcagc agccagatac aaaaaagtca gataagttag
    15541 ttaattgttt aatgattgtt tttttcatga aagattcctc caaaattcat caagtagttt
    15601 gtaataggaa gcaacgtagc tatttctaaa acaatcccaa gtagcaaata ggtagaaaag
    15661 taagaagctg agaaaaataa cgtaatgaga gaaaaaatta gaaacatgct gatcgccttc
    15721 tttaataatg ggggacgttt ctcgggttca atgggattga ttgctgtacc tcttgatcca
    15781 tagcgaagca gtaacaaaaa gcagctgatt aacaaaaaga ccatcattgg gaaggagata
    15841 gtcaattgca gatgtttcat caccaaggga acgccccata aataaagtag tgtccacagc
    15901 agacaaacga attcagagtc ggaatgcaat ccataagcat atctgcggat gcagatataa
    15961 gaaagaaagg tgattcctgt ttccgcccat gagcctgtca ccagagaaaa gactagaacg
    16021 accgctagtt ttccaagatt attcaataaa acttctaaga caaattttac ttggacatac
    16081 accatatctt cttccgattt gtctccgctg aataaggctt ctaataattt gttcacaact
    16141 ttatctttca tctcatacgt acctccacca caaattttat gctaaaaaaa ataaaaaacg
    16201 tcttttgttt cataaaaagt ggttttgaca tcaaaaccgt catgatctcc taaaaaaatc
    16261 ttcgttttcc aagaacctta ccaaaaaaag tgacagtttt gatacgaaaa gtacttttta
    16321 tgacaagttt gtgctttttc gagtgattta tgttaaaaaa ttactgacct tataaaaggt
    16381 gatggaggga ttcttatgga aaatgaacaa ttgtttgaag tcggcgaaac atatgcattt
    16441 aaatctgatt tatttgaatc accaataaaa ggaaaaatag aaaaaatcta tgaaaatagt
    16501 gtgatgatac gtgtcatgaa atgcgcagta gaagacaatg ataccgctca taatctgcac
    16561 tggcagttag ttgtacgcaa atcaagtaca atagaagaat aaaaacagat aaaatgttga
    16621 gagcataaag aggttgtgag tctcccgttt agctctgagt tacaaggaat aatgttaaaa
    16681 aacagctttt catgttttat gaatattgcc ttgtgaccga ggtgttggca ggagaatact
    16741 gtttatttga aaaaagggct gcgacaagac ttttgtcaca gcctttattt tcatgaatag
    16801 gagttacttt tggctacttt tagagaagta gcttttaagg agcagataaa gtcaaggctt
    16861 ttatttctat aaatgaagta gtaaaatgaa gaacagattt tattacagga aggatgccga
    16921 taattgtgat cattgttgca ggaacgatcg gtgcagggaa aagcacacta actgaaatgc
    16981 ttgcccaaga tttagaaaca aaaccttttt atgagaacgt ggaagataat gaagtattgc
    17041 cgctatttta ttctaatcca gaaaagtata cttttttatt acagattttc tttttgaaca
    17101 aacgcttttt ggcaatcaaa gatgctttca gccatgatga caacgtacta gaccgctcca
    17161 tctacgaaga tagtatgctg tttcatttga atgcagattt agggcgtgtt tctgaagtag
    17221 aagtaaagca gtatgaaggt cttttagaaa cgatgctgaa ggagttagaa gaaatttcgc
    17281 cacaaaagaa accagatcta ctggtttata ttcgtgtatc ctttgaaact atgttagcga
    17341 gaatcaaaaa aagaggccgg gaatatgaac agctagaaca agatcctgaa ctctattcct
    17401 actataaaga actcaatcga cgatacgaag agtggtatga acagtttgat atatgtccca
    17461 aaatagtgat cgatggggac aaatatgatt tcgtagccga tccggcatgt ggagaacaaa
    17521 tcgtacaaga aatcaagatg cgggcaaaga aaatgacaga agaggcagat gcccagtcga
    17581 tccgctacac tacaaataac ccgaaagcaa agggaatctt tacaactaaa taatataaaa
    17641 actaagcaaa ttgctcactc gcagatattt ctctgtgagc agcatcttgc ttagtttttt
    17701 tgattcattg agatcaaata gtgaaccgat tcgtatgata aaattgccat aaatgaaatg
    17761 tctgagtgag ggccatgatc aaaaataaaa tggaaacgat caaaatcgaa tgggcgccaa
    17821 acagatgatg acagaaaaca aggacaatag ctcctgcgat aaaggcaaga acgacactta
    17881 gatagtggaa aaatcgatca aaatctgctt tttctttaga aatacaataa tcatataagt
    17941 tcgttgctac cccttttaaa tttccagttg taaataaatt agtgtaggca cgcccgttga
    18001 ttttatcaaa agctacccac tggatcgatg cagtaaaagc aatcaaaatc gttactaatg
    18061 cgggatgctc atggacgggt gtcaaactaa caaagaaaaa aatcactgct tcataatata
    18121 gaacaaacaa gcgccagaaa tgtttttctt tttttctaaa ataatcaatt agatactttg
    18181 ttagaagaat ccctaagaaa aagaaaaggg tagacaatac ttttttacca acacttagcc
    18241 attctccgtc aaatgcttgg atgatcgata gaatgatatt gccagtttga gcagaggcaa
    18301 aagcaccata ttggatataa gtataactgt ccatggctcc tccgataaat gtcagcaaaa
    18361 gtcctattgt acgcccctcg tgaaaggaag tctgcatttt tttcatcacc ttttatgaat
    18421 atttttgtct tccttatctt agcataaacg attgaaaaaa aggaaaaatt ttcagaaaac
    18481 taaaatcata tcgcttccca ttttcatttt tagaaaggtc tgttagtata aagaaaaaat
    18541 agacaacgaa actttaaata accaacttga aataaacaaa ttcaaagaag aaagatggga
    18601 aggaaatcaa tcagttgaat gaaaaagaaa ttctccgatt acaaaaagaa ttgaaacgct
    18661 gggagaacaa gtataaaaag cttcaacaag tctttcggct acaagaagaa atcattgcta
    18721 cctatgaagc ggcttatggc gaagcacccg gacttattga ggaagaagta caagtaaaag
    18781 aaagtgaagg aaacagctct ttctttaaaa tgatggagaa acgttcctat aatcgcttca
    18841 atcaagaaca aaaagcagct atcacatatg atatgaagaa acatcttcga atcatagcag
    18901 gagctgggag cggcaaaacg caaacaatct gtgcaaaagc tgtttatctg atgacgcaaa
    18961 aacaagtaga cgaagaacgc atactaatga tcacttttac aaggaacgct gcaaatgaac
    19021 taaaaaaacg agtagataat ttcagccaga gaaaaaccgc tgtccatatc ggaacattcc
    19081 atagtatctt ttttaggata tacaatgaaa tttgtcgaaa atttcctgaa gtagcaatgc
    19141 aaggaataca aggagacttt tcaaaggata ctgcacaaaa agtgaatgct gttttgcaac
    19201 agctgatacg taaatataat ctttatatat ttgatcaata tggagaaaaa acgattgctt
    19261 ctcggttaga ctattggcag aatatgaacc tttcagtaga agaaatggtt cagttagtta
    19321 aagaaaaata tgattccatt gataagaatg cacgacagcc gatatcagaa cgactgtatg
    19381 gattgctcac ggaactccaa gaacaaaaaa gaagccagca attgctagag ttcaatgatg
    19441 tactccaaaa tctgaaaagc gctttggaga atgaggagat ccagcattat atcggccaaa
    19501 aatatgatta tctattcatt gatgaatttc aagatactaa tccacttcaa tgggaaatcg
    19561 tgaaacggat gacaaaggaa aacaggatca agttgattgt tgttggagat gatgatcaga
    19621 gtatctatgg attcagagga tcagagccag catacatcaa gaactttgaa aaagaatatc
    19681 ctaccaaaac tcttttctta ttaactaatt atcgttctcg ggcagctatt gtccaaggag
    19741 caaatagatt gatcagctat aataaaaatg atcggatacc aaaggcgatg attccagcac
    19801 aaaaagaagc aggtgttatg gaagcctatt tgttttctga tactaaagca gaaagtcagt
    19861 ggctaatcaa acaaattcag acatttattc aaaaaaatgg gaaatataag gaatcaatca
    19921 tattataccg atcagcttca caaacgcagc agcttgtcca atctctgctg aaaacgaata
    19981 ttccttttgt actggaagca gatagtccat atgaaggtat ctttggtatc aagtctttcc
    20041 aacatttcta tcaaaaaatt gttcattggc aaacagcact gaatctgcaa aagaaaaatc
    20101 aggcctacca gcaattgctc cggcagctga tggcagactg ctacctgaaa aaaagtgaat
    20161 gcgatcaata cttttctcct aaatatcaaa atatagccgt tgcagattat attttgtcga
    20221 taagccccaa tttaaaatcg aaatctgcag aaatccgaga gtttgaggaa gcgttgcgta
    20281 aaacagacca gcatcaagaa atctattcgc ttgttcaaag ctatcttcat ctgccacgta
    20341 tagctaagga aatcgaccag agtgatcaag aatggatacg agaagaagta aagcagcatg
    20401 ctactttcag aagactccaa gccttatcgc aagaaaccca agcgacagct aaagaattaa
    20461 aaacccgctt gattgcttac agacaaggaa aacttgatgc actatgtatc cagagcattc
    20521 acaaaagtaa aggtcttagt taccgaaatg tctttttgat tggttgtaac gaaggaagtt
    20581 tgccttataa cggagcgaca gaaaagaaag taatcgactt taatgaaata aaagcagaac
    20641 cagtaacgac gatagaagaa gaacgaagac tattttatgt tgcgatgact agagcaagaa
    20701 atcgtctgta tctatgtgta cctcaaatga aaaattcaag aaaattgaaa gtatcgagat
    20761 ttatcaaaga aaccgggagt cgtgtgaaaa aaggaaagtg atagaagaga gggtgtgaca
    20821 gaagtggtca gtttcaagaa agaagaagga atgtacgaaa attgcttctc aaatttttgt
    20881 gaaatttcag cttattttcg aagaagctat ttctggaaca cagtttattc ggaaaaggga
    20941 ggtgtgacaa gaattttgtc acacctccct tatttatttt gaaaatgaga atactattat
    21001 caaaatagag aatatataga aaaattctca ttattcaaac gtaaataatc agttattttg
    21061 atttttttta tattttatta gctaataatt ggtattttaa aagaaattac tgtagaattg
    21121 gattataatt aaaaaggagt gagaaaatga taaaagtata tacttcgcct agctgtactt
    21181 cctgtagaaa agcaagagca tggttgcaag cgaatcaact tgaatttgaa gagaagaata
    21241 tttttgccga accgttaaca gaaagtgaga tcaaaaagat cttggcagcg actgaaggag
    21301 gagttcaaga tattatttcc actcggtcga aaatttacga aaagctgggc atagatttta
    21361 acgaattaac tttaaagcaa atgatcgtct tattcaaaga atatccttca ttattgagac
    21421 gacctatttt gattgatgaa aaaagaatac taatcggatt taatgaagat gagatccggt
    21481 catttgtccc tcgtgaaatt cgatgtgttg ccagaaatct gatctattta caaaacaata
    21541 acatcttaaa ttcataggga gggagccttc gtggaagtga aagtctatgg atcagcgcac
    21601 tgtgtaacaa gtctgaaggc gaaacaatgg ttagaacacc atcaattgcc ttatcaattt
    21661 gttgacttag aagaacgcga tctaagtaca gaagaagcag aagaattatg cagttttgaa
    21721 gaagtgcaag cagaacagct atttgcgact tggtctgagg cttttcacaa cattgcgatt
    21781 gacctttcct gtgtaggaaa aaaacaactg atttttttat gcacacaaca taccaatctc
    21841 ctcagacgtc ctttgattat tatcgatgaa gttctattta ttggatacaa tgagcagttg
    21901 atggaacagc ttatcttaaa taaatagact ttaactgttt taatcaagtc actgagcatt
    21961 tcatacagtt gtaacagtcg aacacttagc atcaagcgtg ttatgtgttc tttttttgac
    22021 tttatttttc tactatagag acatgctcaa tttatgtggt caaaattcaa atctaggtgg
    22081 ggatatagag ttaaaaatat attgacatgt gtaaatttac atgatatatt catttctgtt
    22141 gttttaagta gcaaagcaat aaaatagtat tttaaaaaac atataaaata gttgttgaca
    22201 tgttagaaat gacatgataa gataactgag ttgttaaaaa agaaccactt gtgaagcttg
    22261 attctatcga aaaaaacttc aaaaaagttc ttgacaaata acaaatgata agttatgata
    22321 aatgagttgc tgtaagattt cgaaaaacga aatcaaaaaa acttttaaaa aagagttgac
    22381 aattacttgt ctctctgata tgatataaaa gttgctacgg cagccaaaaa cgaatcgggg
    22441 aacactcctg atgaagtaga cctttgaaaa ctgaacaaag taagacaaac caaatgtgta
    22501 gggcgtcttg attcaattca agacaacaaa ca
//
LOCUS       DACDIG010000045        22436 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.045, whole genome shotgun sequence.
ACCESSION   DACDIG010000045 DACDIG010000000
VERSION     DACDIG010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.045"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            149..562
                     /locus_tag="IV247_001784"
     CDS             149..562
                     /locus_tag="IV247_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP8777855.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            1758..2303
                     /locus_tag="IV247_001785"
     CDS             1758..2303
                     /locus_tag="IV247_001785"
                     /inference="COORDINATES: protein motif:HMM:NF024252.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8777856.1"
                     /translation="MHMHIGEKIYKLRRLNNETMNDLVKQTGVSKSWLSDIESGKKQR
                     VDFNKICAIAKHYKVSLEYFRDDYLDSDEHEDIIPLTGETRVKSVKSLEQSLKYLNNK
                     QEPNLLEIKLKDTDSVPEVWYKGERLDELPKGLVDVSYHWKTDDFTNDDRGANDITIQ
                     YFSSFNDKYPDKKTIGHKRDM"
     gene            2304..2621
                     /locus_tag="IV247_001786"
     CDS             2304..2621
                     /locus_tag="IV247_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP8777857.1"
                     /translation="MKEVRPRDEIDKLYKTKRWRDLRQVVIARDFGMCQECKRRGRNT
                     RGTIIHHIVEAREDLSLFWSVDNLECICVACHNREHPERSGGKKKPKPKSHIVKMYST
                     PER"
     gene            complement(2778..2942)
                     /locus_tag="IV247_001787"
     CDS             complement(2778..2942)
                     /locus_tag="IV247_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HAP8777858.1"
                     /translation="MGELYKPGEDNKPKGTYKEVGPRGGNVQGGQEVKIDPGDRLPPT
                     SQKGNKWTKK"
     gene            3044..3565
                     /locus_tag="IV247_001788"
     CDS             3044..3565
                     /locus_tag="IV247_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAP8777859.1"
                     /translation="MPQPAKSAKLQLLNGNPNKKNTEELRKRAAAEDKLKMATDKIKP
                     PLWLDSLGKDTFEFIADELLSVDLISNPDVHTMALYSNWYSQYVSLEKQLRKLQREYK
                     LNYALAKKEAEARGEPFNEPNELIGNPLSRQMDTASRNLRSFGADLGLSPAARAKLAI
                     KMADDGGDDDDDF"
     gene            3537..3992
                     /locus_tag="IV247_001789"
                     /pseudo
     CDS             3537..3992
                     /locus_tag="IV247_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010965199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            complement(4032..4190)
                     /locus_tag="IV247_001790"
     CDS             complement(4032..4190)
                     /locus_tag="IV247_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HAP8777860.1"
                     /translation="MDKYGLKNRIRISNAIDKDLYEGLKKMSEETMVPMSKLLDKAIE
                     LLLKESQK"
     gene            4283..5044
                     /locus_tag="IV247_001791"
     CDS             4283..5044
                     /locus_tag="IV247_001791"
                     /inference="COORDINATES: protein motif:HMM:TIGR01453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP8777861.1"
                     /translation="MKKYIIYKHLNKINGKIYIGVTNDIGRRWRSGGIEYKPPKNETQ
                     HHRSFWNAIQKYGWENFDHLIIEEDLTMKEAFEKEKFYIELYDSTNKKKGYNIAKGGN
                     GGIIYKVHPKGMLGKKQSKEFSSNHSKWAKNHKNNCMTNGDVVWGVTHEHPKGMLGKH
                     HSKESIMKKKAYSGENAVTSKPIVAIERDGTKREFHSAKLCMAYYSISTCVFYRLLKD
                     GAPYVINPKANYRNKEKILAIEGIMFKHKEDTEVS"
     gene            5231..6583
                     /locus_tag="IV247_001792"
                     /pseudo
     CDS             5231..6583
                     /locus_tag="IV247_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            6599..7819
                     /locus_tag="IV247_001793"
     CDS             6599..7819
                     /locus_tag="IV247_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP8777862.1"
                     /translation="MSILDRLRSFGRAKPKASKQEYFLNDPGLIPYLVGKDEISEGIF
                     SVISRVSNVFASLPLKMIDVEFGQPDDCPAYNLLSEGPRYFTKFDFFRDVEVLRNYQG
                     NAYVQIFRNINGEVADMALVKPGACHPVIDMDSGELYYQVTATDKGSYKQVIYVHYME
                     MLHFKQPRFGGLEGTDPTKVLTNTLGYDREVRKISLSQLKGSNEGLKVKFASNMDEEA
                     KKATVKNIADFYRQNGGLLVEENGVEIERLQRELVDSKLLDTDKVSRSRIAMVYNVPE
                     HFIGNNQSSYSSQEQLNMEFLTYNLVPTVNQYEAELNKKTLSRTEKAKGYRYKFNVAS
                     LLRADTQARGQFYQIMRRGGAYSANDVRRFEDLQPINKTGMDDYHISGDLYPIDMDPT
                     LRKTTSSKSVAENG"
     gene            7854..8531
                     /locus_tag="IV247_001794"
     CDS             7854..8531
                     /locus_tag="IV247_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAP8777863.1"
                     /translation="MKKVTLSGDVVDNDTAWLYDWFGINCISPGKISAALTEAAGDEV
                     ELDISSNGGDVLAASEIYTAIRAYPGKVSGNVVSIAASAASVIACACEPLRISPTAHI
                     MIHNAWVTTSGNAEELKANAEMLSSVDESIVNAYEIKTGLDRKKLADLMAKDTWLNAQ
                     TAVAEGFADEIMFAEAPVTVLNASQPVIPKNAVTKLKNLILKAEAPQEETLLQKKLKA
                     LNGGKNE"
     gene            8528..9721
                     /locus_tag="IV247_001795"
     CDS             8528..9721
                     /locus_tag="IV247_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP8777864.1"
                     /translation="MNLEQLKNAWVEAGSKVSDLNAQLNAALVDDEKTEEDVVSLQAQ
                     VKAARAKRDGLKEQVANMEAEQVLNVKKEPLDKKDENLKNKFIKDFKAMVNGDPAIMA
                     TLTSNTDESGNAIGLTIPVDVQTTIHTLVRRFDSLQEYVNVEKVTTTSGSRVYEKWSD
                     ITPLTALDTEDGEIPANDDPALYLIKYLIKRYAGISTVTNSLLKDTAENILAWLSKWI
                     AKKVVVTRNTKILAAIDGIKAAQKKDVTDVDGIKDIVNVQLDPAIEATSMFITNQDGF
                     NVLDKVKRADGSYLLQKDVASATGYTFLGKPIKKIASRFLPNKGTQATPKYPLYIGDL
                     KEAVTLYDRENMSLLTTNIGGGAFETDTTKVRVIDRFDVQLVDDEAVVLATFTTIANE
                     TPAKV"
     gene            9736..10062
                     /locus_tag="IV247_001796"
     CDS             9736..10062
                     /locus_tag="IV247_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAP8777865.1"
                     /translation="MILDPKMDLGEIKNALKIDTDDDDVEVSRAAQAAIAYIRGAIGN
                     DKPSFYKQENETVDLINLAILQLADHYYNARSATVSGNLREYDLGFTSLILQLKASYL
                     LFVEEE"
     gene            10066..10401
                     /locus_tag="IV247_001797"
     CDS             10066..10401
                     /locus_tag="IV247_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP8777866.1"
                     /translation="MPLIQTGNLNQRIKFVRDTTVKDEDGQVVPTSTTILTCWASVQT
                     QRLNDIKTSIGTALEGTLTFIIRYQQKSELTNDMKVRWNGKTFEIITITKGEFAKDFT
                     TIIAKEVSK"
     gene            10398..10793
                     /locus_tag="IV247_001798"
     CDS             10398..10793
                     /locus_tag="IV247_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777867.1"
                     /translation="MSVEVDATEVYKALREVKANVQRVESPALRKAGEYAQEELRQNT
                     PYWDGTKSNGKRGSYMQEHAKNHVVTSSIKNGLVEVGYDKDVSWRMHFIEFGTIKQRP
                     KGFVQKTQKQIEKQVTQIIADEVKRRLGL"
     gene            10790..11161
                     /locus_tag="IV247_001799"
     CDS             10790..11161
                     /locus_tag="IV247_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777868.1"
                     /translation="MKTAVSQVYSILNSNEKTKNIDFYTNSVPESAQTVPSLPVGRIT
                     EISGNYEDFASNNPLTIQFNVQVDVWVSTMKEVDAFYFALDEVMRGNGWQCAYTEQTD
                     DEDLEGAKRIIKRYVANISLN"
     gene            11177..11737
                     /locus_tag="IV247_001800"
     CDS             11177..11737
                     /locus_tag="IV247_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP8777869.1"
                     /translation="MATVGFESVIFGVKTGAGGTLKELVADKSKGGAIEAKITGLGAT
                     SNTTYASNVPFFIASKGVSSPKVTLDVADLMDNGIYSEIIGAKTVDGANVIGSETEAP
                     YVSVVMVTANKEGKRLFMGLTKGKFSHPDIDMKTAEDKGVELQTDSIEGEFISDERGY
                     VYLTAVESEGMTLQKFKDLVNNKEGE"
     gene            11862..12173
                     /locus_tag="IV247_001801"
     CDS             11862..12173
                     /locus_tag="IV247_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777870.1"
                     /translation="MIELQLKLDGKKKTFKQQDISARAMRECIKFYEKAEKADLTDLE
                     AIDSMIAITADIFQDPAVTFDAILDGLTASELVPALESVFEQINELGNNEKKQTASKK
                     K"
     gene            12197..12388
                     /locus_tag="IV247_001802"
     CDS             12197..12388
                     /locus_tag="IV247_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777871.1"
                     /translation="MDQIYKDLIEAGWTMRDVDEADYHYLLHLFGEVESGEEYVDGAD
                     FIKQFLSAEDLVKLEEGGK"
     gene            12388..16244
                     /locus_tag="IV247_001803"
                     /pseudo
     CDS             12388..16244
                     /locus_tag="IV247_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922442.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
     gene            16245..17117
                     /locus_tag="IV247_001804"
     CDS             16245..17117
                     /locus_tag="IV247_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777872.1"
                     /translation="MRTVLLRNRINEEIDLSTEDYFATGLSNMGFEVKKEHVGQWGNF
                     RESSESVEISEFQSSVIISVHGFREKELYNSLVQFLSEGPFELEFAFDGETMVRRCSL
                     KSLSKTEIDPKTSLLTDTLELYFTSNWYSVKREKLIQRPNVVKTRGKVFPYKRSYIYT
                     QNLWEKKGVFKFNNNSVYLTNSKERMSPLKIRVIGKCSNPYWEVIQNSQIIATDGYFI
                     DMTETQTLEVSSLFEDTTAILKDIAGVESSVYQQQDYTKTNFVQAPTGEFSIVFHVGG
                     ADVEIELYEERDLF"
     gene            17114..18163
                     /locus_tag="IV247_001805"
     CDS             17114..18163
                     /locus_tag="IV247_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777873.1"
                     /translation="MILAVTLFHRDLMLYNEHLFSNSFEFGVDEINEEASSFTMDKYV
                     PVKTGDFLLAKYIPSGKFAYFGVITSQEDEKISCKSLLSLADSEIPTARVSGDNYEEH
                     IRRLIEYYLLNDPTKQLKDILDVKAESATSHSYQATDTNKHKLSAYILNGFKKYNVKW
                     YFKGIQNRKIYTGIRAVNESIYIKDNSSEFSDWDVFVQAPGAGNENKLLIVDKAMKDI
                     EKPIILSTWYLDEENNLTQDGSKENITKPTVNLVNIYDQTAEDKASYEDVAKSELKGN
                     TYSHEIKVNVVRNAKNLNVETIETGMFATISYKGKIYKSVLTAWRISSDKEFVELTFG
                     NIRSRFMDYFEDNGG"
     gene            18167..18991
                     /locus_tag="IV247_001806"
     CDS             18167..18991
                     /locus_tag="IV247_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777874.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERV
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            19009..21069
                     /locus_tag="IV247_001807"
     CDS             19009..21069
                     /locus_tag="IV247_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HAP8777875.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDQTNQQIGDLGKLKKMYSNSIDFGGYDYSGNPNL
                     LSKLSYDLIENQNTSAGTLSKGENSFKYNKISAEVEGGVELYYKRRGIANWLPSNKTL
                     VMTVKLRAGADYSPVDGKLILIRYRYVDSGTGKIVLDLPINSNSITQEWKEFSITGTT
                     PTFSPQAYHPWIQFRAQDGVLGEIEMSYDIKIEEGSTATPYQPNLLDDSYWLGKTPLG
                     ENLITNAKFPIITRSNPISGFDISEELIIGETYTVSLKGTKPATKEFHLYYGEANYQQ
                     LQYQATLLPVEGLTDVWSATFTARNTSETTNLLRVALWQKPNSATYDTVQIDWLKIEK
                     GDTRTPNISQFKYFGEGLKDSNNPNDYSWDVTPEYTEKSLNNTVSLTEPQSIEGLKNF
                     EDGIQSKGKSVLTSDDNKYEVVTLTVTNGNTGSAKLYREGKTVTIYFFALNGKSSGGN
                     DSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAI
                     TYTI"
     gene            21232..21678
                     /locus_tag="IV247_001808"
     CDS             21232..21678
                     /locus_tag="IV247_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777876.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            21680..21817
                     /locus_tag="IV247_001809"
     CDS             21680..21817
                     /locus_tag="IV247_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP8777877.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADRIISKEESA
                     S"
     gene            21852..22094
                     /locus_tag="IV247_001810"
     CDS             21852..22094
                     /locus_tag="IV247_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP8777878.1"
                     /translation="MKEEALQDVVERLVRIETKLDNYESLREKADSAKDLADKAYSVA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            22110..22307
                     /locus_tag="IV247_001811"
     CDS             22110..22307
                     /locus_tag="IV247_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP8777879.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            22318..>22436
                     /locus_tag="IV247_001812"
     CDS             22318..>22436
                     /locus_tag="IV247_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8777880.1"
                     /translation="MKKKIILSLSLIMALFLLPINGFAYTINNEFNLGVNEGSS"
BASE COUNT         7770 a         3798 c         5006 g         5862 t
ORIGIN      
        1 aagataaaaa aataagtaag gcttctcatt aaaatataaa aaagacagcc gaccactgac
       61 tgcctatata agagtattga aataaaaagc tgctgatata ataaattcca caagtttatt
      121 atatcacata aaggagcggt ttgacttgat gcaattgtta cgagaggtag atttcaaaca
      181 gacaagatgt aatgcgagag atgtgctgaa gaactttcgg cgtttggagc ggatggcagg
      241 tcgctctttg atagatatta agtcgccgat tattacggat atgccgaagg cgccgaagca
      301 cggcaataag gcagaagacg caatcattca gatgatggat atagaagcgg agagagacgc
      361 gattctagca gccttgatgg ctcttagtct gattagtcgt cagatactct actatagctt
      421 ttgtgtgcca gatagcttct caaactacag aattagccgt gaagtgggtt attcagaaag
      481 aagtatacaa cggatgaagt cggaagctct aatagagttt gcagaagcat ataaacacgg
      541 aagaataatt gcttataaat aatttggcgg ctttttggcg gaatgatggc ggtttttagc
      601 tatttaccag tgatattatg gtagtgtcga aagattagtg ataggtctaa gacaaaataa
      661 taataaaagg aacatcgttt tattattgtt tcacaattaa gcttcgatag acagcaacgg
      721 aaatattaag aataaggatg tgaattttaa ctccttctaa attgttctta ttatctatca
      781 tccgttgctg tctattgttt ttttaattat tcacacgata actaaaggtg agcgaagaga
      841 aatgattcca ttaataattt caatttttgc gctttgtctt aatgtctata tgattggatt
      901 taaaaatggg caaaataaaa gatagtagta actaagaaag aaatttttat attgtcactg
      961 tggcggaaag ggtagacgct aagcatgtgt gctaggtcaa tgcttcggca actatgcaat
     1021 gttcgattca ttgccagtga ctttggcaac cgaggcatcg gcggtttaaa aatatagggg
     1081 tgcgcaattt cgtacgcgtt ttgtgcatcg tgtaggttgc tattacatat tagatcactc
     1141 gttgagtggt ctttttattt tacataaagg aggtaacaac gatgtataga ccacaatact
     1201 tagaacagaa gtatgaagta atcactgtgc aaaacggtaa cggtgagata gtacgaaagt
     1261 atagaagacc aataaagagc gatacatata aacgaaagga aaacaatgaa gttatttctt
     1321 ttcgcagaag gagaaaagcc aaatgagaaa ctactggtat gtatcgctaa ctaatgaata
     1381 tcctcgaacc attgatgatt gttcagtgcg tgttgtgcgt tctgtacaaa tcaaagggaa
     1441 gtactctatt gtcgaaatgc taagagaagc tacaccaaac gaagtggata aatgcaagct
     1501 gatatattgc ggtcatggct attggaaaga cgagtatatc caacaaaaca ttgagaggtg
     1561 gatagataga tgagttacct cgaacatttg aaacgttgct acatgcattc taagaataaa
     1621 cttcctgaca gctacactgt agatgatgtt gctatacatg tattgaagac tgagagccat
     1681 agcagtcctg atggtagcag taaggagcag acgctcgcgt ggttcaagtt ctttaagtgg
     1741 attaaggagg aagagtaatg cacatgcaca taggagaaaa gatatataaa ttgagacggc
     1801 ttaataacga aacaatgaac gacttagtta aacaaactgg agtgtcaaaa tcatggttat
     1861 ctgatattga gtctggaaaa aagcaacgcg ttgattttaa taagatatgt gcaatagcca
     1921 aacattataa agttagctta gaatactttc gggatgatta tttggatagt gatgaacacg
     1981 aggatattat tcctttgaca ggcgaaacaa gagttaaatc agtgaaatcg cttgaacaaa
     2041 gcctaaagta tctgaataac aaacaagaac ctaaccttct agagatcaaa ctaaaagata
     2101 ctgactcagt accagaggtt tggtacaagg gtgagaggtt ggatgaattg cctaaaggat
     2161 tagtagatgt ctcgtatcat tggaagactg atgattttac taatgatgat agaggagcga
     2221 acgacatcac gattcaatac ttttctagct ttaatgataa gtatccagat aagaaaacaa
     2281 tcggacacaa gagagatatg taaatgaaag aagttagacc tagagacgaa atagacaaac
     2341 tatacaagac caaacgatgg cgagacctaa ggcaagtagt aatagctagg gacttcggca
     2401 tgtgccaaga gtgcaagcgt cgagggcgga acacaagggg cacgatcatc catcacatag
     2461 tcgaggcgag ggaagacctg tcactgttct ggtccgtaga taaccttgaa tgtatctgtg
     2521 tagcttgtca caacagagag catccagaga gatcaggcgg gaagaagaaa ccaaaaccta
     2581 aatcacatat cgttaaaatg tattcaactc ctgaaagata agtttgcagc gaaatgaagg
     2641 tagcccccct actctaaaag attaaagagt aaggcttgag aagaacggtg ctgtccttcc
     2701 ttcgtaaaaa gaccgctttt caagtttttt ggagaaaaag gaaaaagccg accaatttaa
     2761 gccggctttg gacgaagcta tttcttagtc catttgtttc ctttttgaga agtagggggt
     2821 aatcggtcgc ctggatcaat ttttacttct tgtccgcctt ggacatttcc accacgagga
     2881 cccacttctt tgtaggttcc ttttggtttg ttgtcttcgc cgggtttata gagttctccc
     2941 ataggaatcc ctccttaaca aatttcggca cagcactgcc gataacctaa ttataaggat
     3001 tgtgataacg atttcaatct atcttttgaa agaaggtgat attatgccgc aaccagcgaa
     3061 gagtgcaaaa ttacaattat taaacggaaa cccaaataag aagaataccg aagaactccg
     3121 caagcgagcg gccgcagaag acaaattaaa aatggctact gacaaaatca aaccgccgtt
     3181 atggctagat tcgctaggaa aggatacctt tgagtttatc gccgatgaat tgctgtctgt
     3241 ggatttaatc agtaatccgg acgtccatac aatggctcta tactccaatt ggtattcgca
     3301 atacgtttct ttagaaaaac agcttcgaaa actacaacga gagtacaagt tgaactatgc
     3361 gcttgcgaaa aaggaggcag aggcgagagg tgagccgttt aatgaaccta atgaattaat
     3421 tggtaatccg ctctctcggc agatggatac agcgtctcgg aatctccgtt cttttggcgc
     3481 tgatttagga ctatcaccag cagccagagc taagttagct attaagatgg ctgatgatgg
     3541 tggtgatgac gatgacgact tctaatattt tggatatgtc ttacacagaa cgtgtggact
     3601 attggcaaag ctatcttgag gagcaagctt cttggggtgg ctttttaaaa tgtccatatc
     3661 cggaattgtt aactacttgg tatgcggaac gattaatcga tggaagcata ccagccagca
     3721 aagaaaatat tcaagctgct aaacggcata tgcgtgattt gcagcgccaa ggaacagatg
     3781 attttccttg gatctttgac gaagaaaaag gtcaccggcc tattagatat atcgaaaaaa
     3841 aatgtaaacc aactgaaggc gactttggtt cgtttgtttt gcaaccttgg cagcatttca
     3901 taattggatc catgtacgga tgggtacatc gtgatacagg agagcgtcgc ttccgcgagg
     3961 ctcttatttt tgttggacgt aaaaacggta agttttgccg tttaaaaatc gggcaaaatc
     4021 ggtgaaaacc tttatttttg gctttctttt agtaataatt cgatagcttt atcaagcaat
     4081 ttagacatag gaaccatagt ttcttccgac atttttttta atccttcgta taaatcttta
     4141 tcaattgcgt tagaaattct aattctgttt tttagtccgt atttgtccat gtgtgacact
     4201 cctttttatt ttactataac atccgattcc accgcttgca actaccatca attgatggta
     4261 gtatataaaa gaggtgagta gattgaaaaa atacattatt tacaaacact taaataaaat
     4321 aaatggcaaa atttatatcg gtgtcacaaa tgatattggc agaaggtgga ggagcggtgg
     4381 gattgaatat aaaccaccaa agaacgaaac ccaacaccac agaagttttt ggaatgcaat
     4441 acaaaaatac gggtgggaaa attttgatca tttgattatt gaagaagact taacgatgaa
     4501 agaagctttt gagaaagaaa agttttatat tgaactttat gactcaacta ataagaaaaa
     4561 aggttataac atcgcaaaag gcggaaatgg cggaataata tataaagttc accccaaagg
     4621 aatgctaggg aaaaaacaat ctaaagaatt ttcttcaaat cacagtaaat gggcaaagaa
     4681 ccataagaat aattgtatga caaacggtga tgttgtatgg ggtgttactc acgaacaccc
     4741 aaaaggcatg ttgggaaagc accacagcaa agaatcaatt atgaaaaaga aagcatatag
     4801 tggtgaaaat gctgtaacct caaaaccaat tgtcgcaatt gaacgggatg gaactaaaag
     4861 agaatttcac agcgctaagc tttgcatggc ttactattcg attagtacat gtgtttttta
     4921 ccgactttta aaagatggcg ccccttatgt gataaatcct aaagcgaact acagaaataa
     4981 agaaaaaata ttagctatcg aaggaattat gttcaaacat aaagaagata ccgaggtaag
     5041 ttagtagatt gcgaaaggtt actaactacc gtaacgcgta ggagttgaat aaatataatg
     5101 ctcccaagag tgtccgacag cagataattt gctgaaaatg tacgctaaac tgggccagaa
     5161 ctgactgacc gatgaaaatg agggtgacct ccagagtgcg agataaaaag ctcgcagata
     5221 ataacaatcg aaaacatcac taatttcagg cctttccaca tacatggtcg cttatgatga
     5281 tgaacaaggc gccaacgttt acgtattggc aaatgctcgt gatcaagcaa gcttgttgtt
     5341 tgataaggcc gcagaaatgg tcaaacaatc gccggcgctc tttaagaaat ttggtaagcc
     5401 taaacgatca agtattaatt atgctcccgc cttttctaaa atggaaccac gcgcctcaga
     5461 tagccggaaa ttggatgggc taaacactca ctttggtatt tttgacgaga tccacgagtt
     5521 tacgaattac aatctgatca acgttatcaa gaaatcaaga ggaaccagaa aacagcctct
     5581 gatagtttat atcacaactg ctggatatgt attagatggt ccgttgatgt cttattttga
     5641 gcaaggtgtg gactgtttgg aacatttgga agatgacatc gatgaacgga ctttctatta
     5701 tctggcaaaa cttgacagtg cggaagaggc tgatgaccca agattatgga tcaaagccaa
     5761 tccgaatatt tgtctaatga attttgttgg catgctagat gactatgtta aggataaaaa
     5821 agatccaaaa gaatatgctg actggattac caagcaattt aacttgtttt ccgatatcga
     5881 tgagctgtca tttgtcgata tgcctaccat taaacgaaac aataaaacca tcgatattga
     5941 aacgctcaaa ggtaagaagt gtgtcggtgg ttttgacttg tccgaaacgg aagactttac
     6001 cgcagccgtt ttagaatttc cgcttgaaac aggcgaggta ttcattttgc aacacacatg
     6061 gatcccacaa gctagatttg atcgagataa caatcaagag cgtatcaaag cgtgggagaa
     6121 ggtgggagat ctaacgatta ttcctggtga ttacgtcaat tatgaatacg tcttaaattg
     6181 gtttgtagaa aattcgaaaa tctatgacat tgtaaaaatc aattatgaca aggccaaggc
     6241 gctacgatta aataaagaac tagaaaatgc aggatttgaa accaacgaga ttcggcaagg
     6301 gtttctatca ttaggtgggc caatgcaaaa cttcaaggaa atgctattgg acggtaaggt
     6361 gattttcaac aattccaagc tttaccgatg gtatctatcc aacgtcaagc tggtgatgga
     6421 tcgcaactca aactggatgc cgtctaagca gtccaagagt agaaaaatag atggttttgc
     6481 agcaagtttg aacagccacg ccgaagtgtt gaatatgttg gttaatcctg tcggaaccgg
     6541 gaaagtaacc tattactcga tttccgattt aatgaatatg taagaaaggt gtggaggaat
     6601 gagtatttta gatcgtttgc gttcttttgg ccgagcgaag ccgaaagcga gcaaacaaga
     6661 gtattttttg aatgacccgg gattgatacc gtatttagtc ggaaaagatg aaatatcaga
     6721 agggattttt tccgtaatta gccgtgtatc gaacgttttt gcgtctctcc ctctcaaaat
     6781 gatagatgtg gagtttggcc aaccggacga ctgtcctgca tacaacttgt tgagcgaagg
     6841 ccctcgatat tttacaaagt ttgatttttt ccgggacgtg gaagttttga gaaactacca
     6901 agggaatgcg tatgtgcaga ttttccgaaa tatcaatgga gaagtagcag atatggcgtt
     6961 agtaaaacct ggtgcttgcc atccagtgat tgatatggat agcggggagc tttactacca
     7021 agtaactgcg actgacaaag gcagttacaa gcaagttatc tatgtacatt acatggaaat
     7081 gcttcacttt aaacaaccga ggtttggcgg cttggaaggt acagacccca caaaagtatt
     7141 aacgaatacc ctcggatatg atcgagaagt ccgaaaaatc tctttaagtc agcttaaagg
     7201 aagtaatgaa gggctaaaag ttaagtttgc tagcaatatg gatgaagaag ctaaaaaagc
     7261 tacagttaaa aacattgctg atttttatcg acaaaacggt ggactacttg tggaagaaaa
     7321 cggtgtagaa atcgaacgtt tacaacgaga gctggtagac agcaagcttt tagatactga
     7381 taaagtatct cgctccagaa tcgctatggt ctacaatgtg ccggagcatt tcatcgggaa
     7441 taaccagtcg agttactcct ctcaggaaca gctcaatatg gaatttttga cgtacaatct
     7501 agtaccgacc gttaatcaat atgaagcgga actaaataag aaaacactat cgagaaccga
     7561 aaaagcaaaa ggttatcgat ataagtttaa tgtcgcaagt ttgctaagag ctgacactca
     7621 ggcaagggga caattctatc aaatcatgcg acgaggcgga gcatactcag cgaatgatgt
     7681 ccgaagattt gaggacttgc aaccgataaa taaaaccggt atggatgatt accatatttc
     7741 cggagaccta tatccaatcg atatggatcc aacattaaga aaaacaacct cgtctaaaag
     7801 cgtagccgaa aacggttagg tttttttagt ttgcaccgaa gggaggtgga aaaatgaaaa
     7861 aagtgacgtt aagcggcgat gtcgtggata acgataccgc gtggctttat gactggtttg
     7921 ggatcaattg tatctcacca gggaaaattt ctgccgctct tacagaagca gcgggggatg
     7981 aagtagaact tgatatctca tcgaacggtg gggatgtcct agcggcaagc gaaatatata
     8041 ccgctatccg tgcctatccg ggaaaggtat ccggaaatgt tgtgagtatt gcagcaagcg
     8101 ctgcaagtgt aatcgcttgc gcttgcgaac cgcttagaat ctcacctacg gcacacatca
     8161 tgattcataa cgcatgggtg accactagtg gcaacgctga ggaattaaaa gccaatgcag
     8221 aaatgttaag cagtgtggat gagtctattg ttaatgctta cgagatcaaa acaggactcg
     8281 atcggaaaaa acttgctgat ttaatggcga aagatacttg gttaaatgct caaacagcag
     8341 tagcagaagg gtttgcagat gaaattatgt ttgcagaagc accagtaacg gtactcaatg
     8401 cctctcaacc ggttattcca aaaaacgcag taactaagtt gaaaaattta atactcaaag
     8461 cggaagcacc gcaagaagaa acactcttac agaaaaaact aaaagcctta aatggaggga
     8521 aaaacgaatg aatttagaac aattaaaaaa tgcgtgggtc gaggcgggaa gtaaagtctc
     8581 tgacttaaat gcacaactca atgcagcatt ggttgacgat gaaaaaacag aagaagatgt
     8641 agtaagtttg caagcacaag taaaagcagc acgggctaaa cgggacggat tgaaagagca
     8701 agtggcaaat atggaagccg aacaagtctt aaacgtcaaa aaagaaccat tagataaaaa
     8761 agatgaaaac ttgaaaaaca agtttatcaa agactttaaa gcgatggtca atggtgatcc
     8821 tgctattatg gctactttga catctaatac ggatgaatct ggtaatgcta tcggattgac
     8881 tattcctgta gatgtgcaaa cgactattca tactttggtt cgtcggtttg actctttgca
     8941 agaatacgta aacgttgaaa aagtgaccac taccagcggt tctcgcgttt atgaaaaatg
     9001 gtctgatatt acaccgttga ctgctttgga tactgaagac ggtgaaatcc cagcaaatga
     9061 tgatcctgca ctttacttga tcaaatactt gatcaaacgc tatgcaggta tttccacagt
     9121 aactaacagc ttgctaaaag atactgccga aaacattttg gcatggttgt ctaaatggat
     9181 cgcgaaaaaa gtagttgtta ctcgcaatac aaaaatcttg gcagctattg atggaatcaa
     9241 agcggcacaa aagaaagatg ttacagatgt tgatggaatt aaagatatcg taaacgtcca
     9301 acttgatcca gctatcgaag ctacatctat gtttattaca aaccaagatg gcttcaatgt
     9361 tttagataaa gtgaaacgtg ctgatggatc ttacttgtta caaaaagacg tagcttctgc
     9421 aactggatat accttcttag gtaaaccaat caagaaaatt gcttctcgtt tcttgccaaa
     9481 caaaggtacg caagctactc ctaaatatcc actgtacatt ggtgatctga aagaagccgt
     9541 tacattgtat gatcgcgaaa acatgagctt gctgacaacg aatattggtg gtggagcttt
     9601 tgaaacagac accactaaag tacgcgtcat cgatcgcttc gatgtgcaac tagttgatga
     9661 tgaagcggtt gttttggcta cttttacaac tattgcgaac gagacaccgg cgaaagttta
     9721 aggagctgat tccttatgat tcttgatcct aaaatggatt taggcgaaat caaaaacgca
     9781 ctaaagattg ataccgatga tgacgatgtg gaagtaagcc gtgcggcaca agctgcaatt
     9841 gcatacatta gaggggctat cgggaatgat aagccctctt tttataagca agaaaatgaa
     9901 acggttgatc tgattaattt agctatcctg caattagcag atcattatta caacgctcga
     9961 tcagcaaccg tgagtgggaa cttgcgagag tacgatttag gttttacaag cctaatcttg
    10021 caactcaaag caagttattt gctttttgtg gaggaggagt agcgtatgcc ccttatccaa
    10081 acaggaaatt taaatcaacg catcaagttt gtccgagata cgacggttaa ggatgaggac
    10141 gggcaagttg tcccgacttc tacaaccatt cttacttgct gggcaagtgt gcagacacaa
    10201 cgcctgaacg atattaagac gtcgattggt acggctttgg aaggaacact gacgttcatt
    10261 atccgctacc aacaaaaatc agagctgacc aatgatatga aagtgcgttg gaatggaaaa
    10321 acgtttgaaa tcattacgat tacgaaaggc gagtttgcga aggacttcac gacaatcatt
    10381 gcaaaagagg tttcaaaatg agtgtagaag tcgatgcaac cgaagtgtac aaagcgctta
    10441 gggaagtaaa agcaaacgtt caacgagtgg aaagcccagc acttagaaaa gctggggagt
    10501 acgctcaaga agagttacga caaaacacac cttactggga tggaacgaag tcaaacggta
    10561 aacgtggttc gtatatgcaa gaacatgcta agaaccatgt ggttacaagc tcgataaaaa
    10621 acggattggt agaagtcggc tatgacaaag atgtttcttg gcggatgcac tttatcgagt
    10681 tcggaacaat caaacaacgt ccaaaaggtt tcgtacaaaa aacacaaaag caaatcgaaa
    10741 aacaagtaac acaaatcatt gctgacgaag taaaaaggag gctaggactt tgaaaacggc
    10801 agtatcacaa gtctattcaa ttctgaatag caatgaaaaa acaaagaaca ttgattttta
    10861 caccaatagt gttccggaat cagctcaaac agtacctagc cttccagttg gcagaattac
    10921 agagatatcc ggcaactatg aagattttgc aagcaacaat cctttgacca ttcaatttaa
    10981 cgtacaggta gatgtatggg tgtcaaccat gaaagaggtt gatgcctttt attttgccct
    11041 tgatgaggtt atgaggggga atggttggca atgcgcatac acggaacaaa cagatgacga
    11101 ggacttggaa ggtgcaaagc ggattatcaa acgatatgta gcaaatattt cactaaatta
    11161 aaaggagaga aaatagatgg caacagtagg attcgagagc gtcatttttg gcgtaaaaac
    11221 aggtgcaggc ggcactctaa aagaattagt agcagataag tcgaaaggcg gagcgatcga
    11281 agctaaaatt actggattag gcgcaacttc taacacaaca tacgcttcaa acgtaccgtt
    11341 cttcattgca agtaaagggg tttcgtcgcc aaaagttacg cttgacgtgg cagacttaat
    11401 ggataacggc atttacagcg aaatcattgg tgctaaaacc gtggatggtg caaatgtaat
    11461 tggttcagaa actgaagcgc cttacgtgtc ggtagtcatg gttacagcga acaaagaagg
    11521 aaaacgcttg ttcatgggat tgacaaaagg aaaattcagt catccagata tcgacatgaa
    11581 aacagctgaa gacaaagggg tagaattgca aaccgattcc atcgaagggg aattcatttc
    11641 tgatgaacgt ggctatgtat acttaacagc cgtagaatca gaaggaatga ccttacaaaa
    11701 attcaaggac ttggtaaata acaaagaggg ggagtagtta accctgcatc tacaccaacg
    11761 acagatacag ggacaccaaa agaaccagaa ccaaaaattg atacacaagg ttagccattt
    11821 ttggctagcc ttattttttg taaaaacaag gaggaaaaca aatgattgaa ttgcaattga
    11881 aacttgacgg aaagaaaaaa acattcaaac aacaagatat ttccgcacgt gcaatgcgtg
    11941 agtgtatcaa attttacgag aaagcggaaa aagcagacct aactgattta gaagcgattg
    12001 attcaatgat tgcaattaca gcagatattt tccaagatcc agcagttaca tttgatgcta
    12061 ttttagacgg tttgactgcg agcgagttag taccggcatt agaaagtgtt tttgaacaaa
    12121 taaatgaact gggaaacaat gaaaaaaagc agacggcgag caaaaagaaa taagtttttc
    12181 tgaagctagg aaagcaatgg atcaaatcta caaagattta atcgaagcag gttggacgat
    12241 gagagatgtg gacgaagccg actatcatta tttgttacac ctttttggag aagtggagag
    12301 tggcgaagaa tatgtagatg gtgctgattt catcaaacaa tttttatcgg ctgaagactt
    12361 agtaaaactt gaggaaggag gtaaataatg gcaggaaaag gacaaccggc aggaaatatc
    12421 aagctaggga ttagtttaga tagcactagt tttggtaaca cgctggacga aatcaatgcg
    12481 aaagtcaaac aagctgagtc gaatatgcgt gccaatctaa aggcttatga ttcagcagga
    12541 cgttcatacg aagcacttag tcaaaagacg aaagacttgt ctacggttat ggaagggcaa
    12601 aacgccaaag taagagaatt aacaaagcgc cgtgatgaag cgattagcaa gtatggcgag
    12661 gaatcgaaac aagttgctaa ccttaacaca cagataaaca atgctaccgc aaaatataat
    12721 gcttacagtc gccagttgaa cgacacaaaa aaagaattgg tgtattccaa aacagccgtc
    12781 aatgatttat ctaatgaaat caaagaaaat gaacgacaaa tgaacgccga agtaaaagcg
    12841 ttgaaagccg ctggtgatga atctggtgct tttgaagcaa aacaaaaagg gctagccaaa
    12901 caaacggaat tatccgagaa agctatcgaa gaacagcgca aagttgtgaa actgatggct
    12961 gatgagtttg gcgattcagc aaatgaaacc gaagatgcaa aaagggcatt agaaaagtta
    13021 gaacgacaaa gccaaatatc tagcaggcaa ttagaagcac tcaaaagctc cagcgatcaa
    13081 tcaggaaaag aaatagaaga ttttggcgac aagtccacaa ggtcagctag gaaactggac
    13141 ggactaaaag acaaattaag ctcgctaaaa agcgcatttt cgtttggtgc agttgctgga
    13201 ttagcgcata acgctattag cagtgtagta agtggcgtgc aaggtttggt tggcgaagca
    13261 gtaaacgcat cggattcatt gatgaagttt tccaaaacca tggagtttgc taactttggg
    13321 aagtcacaga tagaaagctc gaaaaaagaa atgaaagact acgccgataa gacggtttat
    13381 ggtttagaag aaattctgaa cacaaccgca caattggcat ctaatgggat tcctaactat
    13441 acagaactaa ccaaggcggc aggtaacttg aatgccgttg caggcggttc tagtgataca
    13501 ttcaaatccg ttgccatgat gctaacgcag acggcaggag ctgggaaact aacaactgaa
    13561 aactggaatc aattagcaga tgcgataccg ggtgcttcag gattgttaca agacgctatg
    13621 ttgaaaaacg gagcttatac aggaaacttc cgtgatgcaa tggcgcaagg tcaaatcact
    13681 tccgacgagt tcaaccaagc aattgtacag ttaggtatga atgacggagc agttaaggca
    13741 gccacttcta cagacacatt gagcggttct tgggagcaga tgaaatccac tgtaataaat
    13801 gggctacaaa gtattataga aaaaataggc gttgaaaata tcactggttt catcaacaga
    13861 gtaacaaaag ggattgaaaa ttctattcct aaaattactc aatttatagg ttggttgaga
    13921 gatattggaa cgtggatcgt tgaaaataga gagccactaa catggattgt cggaatcata
    13981 ggcggaatta cattagcagt aaaagcattg aacgtagcaa gtatgttgct ggcaattact
    14041 ggcggaacat tggcagcccc ttttgtggcg attggtgtag cattaggcgc actagcaggt
    14101 gctttggtga tagcttatac aaaatctgaa acatttagaa atatagtcaa tgcggctttt
    14161 acagctgtga aaaacgtagt tatgagcgtt atcaataatt tggtggaata ctacaaaatg
    14221 ttgtggagcg tgttgcagtg gctttgggaa aaaataaaag aatgggcttc atggattggc
    14281 aataaattca ttgaaatgaa gaacagcgtt gtgaatacgg ttcagaacct atggaatggc
    14341 gtgaaaaact tcttcagcaa tggcgttgga gatacgtgga ataaggtagt cggttgggta
    14401 aaaaacattt tcaacaaagc aactgaattg aagaacaacg tttctgatgt aatcggtaac
    14461 ctgtggaatg gtatcaaaga cacattccgt agaggtatcg atacggtatt caattggttt
    14521 tcagaactac caacgaagat gaagaatgcc attattggcg gtaaaaacgc cattgttgat
    14581 gcgttcaaaa gtattttcaa cgcagcactt aaagcgatag gtaaaccagt taacgcaatc
    14641 atccatggag cttcatgggt actagaaaaa ttgggcgcag aacctctaca agaatgggat
    14701 gtaccacaat acgctacagg tacaccagca ggtgggcacc caatcaatgg tccaatgatg
    14761 gttaatgatg gacgtggagc agaaacagtt atcacaccag atggtagagc cttcattcct
    14821 aaaggacgca atgttgttct aaacgcacca aaaggaacgc atgtcttgac agcagaagag
    14881 accgcgcaac ttcaaggttc taaagctcct aagtatcgtt acaaaaaagg tactaacttc
    14941 tttggtaaca tgtgggatag tgtgaaaaag gttgctggta atgtaggtca cacaattaaa
    15001 aacgtagtag gtgacgtgtg ggactttatt tcagacccgg gagcgttagc tagaaaagta
    15061 cttggggggt tagatgtatt aggtggattg acaaagtatc cattagaagt aggtaaaggc
    15121 atcctatcta aagcaacaag tgcactgact gaaaaaatca ctgggttgtt ctcatctggt
    15181 aacttagata cctccgtagg aacaaatggc gtctataaat atttggctga tgttgctaag
    15241 tctgtgatga agaaattccc aggatttgtg gcaactagtg ggtatagacc tatagaccag
    15301 gtgaccctta ttcacatggt aaacgtaatg ccattgatat tgcattacca ggcgtcacag
    15361 gaggctcacc tagatacaca gaagcagcaa actacgcttt tgacaaattc gcttccaaaa
    15421 ttggttacgt aatcactaat gggaaagttc gtgaccgttc aggacaatca ggtcaaccag
    15481 caactggtgc atgggagcca tggcccgatg gagatcacta tgatcatgtg catttaaacg
    15541 gtgtgaaaga cccacaaaac actcaaattt caggagatag cgtgggaggc agtggggtag
    15601 aaagatggcg caatgtagca attagagcgt tgaaaatgac cggtcaatac agtactgcaa
    15661 acttaaatgc attactaaat caaatgcgta cagagtcaaa tggtaatcct aatgcagtta
    15721 acaattggga tattaacgcc aaaaatggaa caccatcaaa agggttgctc caagtgattg
    15781 acccaacatt cagacagtat gcaatgccag gattcaacag caatattttt gacccactat
    15841 ctaacatctt agcttcaatc agatacgcac tatcaagata tggctcacta acaaatgcct
    15901 atcgtggagt tggttacgca aacggtggaa ttgtaaacca acatcaaatt gcggaaatcg
    15961 cagaaggaaa caagccagaa attattattc cgttagataa ggctaaacga tcaagagcga
    16021 tgcaattatt gtctatcgca atggacaaac taggagtttc tcctaaaact tatggaaatg
    16081 cgacaactgt ttccagtgat tacgaaatcc tagaagccat tgaaaaacag gcggcaacaa
    16141 cgaatcagct gttatcgtta ttgctggcat ttttcaaagg aaatagccga actgatagag
    16201 atttagcttt agatattcag aagatcttgg ttaggaggat gtagatgcga actgtattac
    16261 tgagaaatag aataaacgaa gaaattgatt tgtccacaga agactatttt gctactgggt
    16321 tgagtaatat ggggtttgaa gtaaaaaaag aacatgtggg acagtggggg aatttcagag
    16381 aaagcagtga gagcgttgaa atatctgaat ttcagtcatc tgtaatcatt tctgtgcatg
    16441 ggtttcgaga aaaagaactg tacaattcac ttgtgcagtt cctatcggaa ggtccgtttg
    16501 aactggaatt tgcttttgac ggcgaaacaa tggtaagaag atgcagtcta aaatctttaa
    16561 gtaagactga aatcgatccg aaaacatcgt tactaacaga cactttagaa ctatatttca
    16621 catcaaactg gtattccgta aaacgcgaaa aattaattca gcgaccgaat gtagtgaaaa
    16681 cacgtggtaa ggtttttccg tataaaagat cttatatcta cactcaaaac ttgtgggaga
    16741 aaaaaggtgt attcaaattc aataataatt ctgtatacct aacgaatagc aaggaacgga
    16801 tgtccccgct aaaaattcgt gtgattggga agtgttcaaa tccgtattgg gaagtaatcc
    16861 aaaactcaca aatcatcgca acggatggat acttcataga tatgactgaa acacagactc
    16921 tagaagtatc aagccttttt gaagatacaa cggcgatttt aaaagatatt gcaggggtag
    16981 aatcttctgt ctaccaacaa caggattata caaaaactaa ttttgttcag gccccaactg
    17041 gagaattcag cattgtgttt catgttgggg gggcagacgt agagattgag ttatacgagg
    17101 agcgtgatct gttttgattt tagcagtcac gctttttcat cgtgatttaa tgctttataa
    17161 cgaacattta ttctctaaca gttttgaatt tggtgtggac gagatcaacg aagaggctag
    17221 tagctttacg atggataagt acgttcctgt aaaaacaggc gattttctac tagcaaaata
    17281 tattcctagt ggaaaatttg catattttgg tgtaattacg tcgcaggaag acgaaaaaat
    17341 cagttgtaaa agcttactta gtttagctga tagtgagata ccgactgcac gtgtgtcagg
    17401 agataactac gaagagcata tccggcggtt gattgaatac tatttgctga atgatccaac
    17461 gaaacaacta aaagatattt tagacgtcaa agcagaaagt gcgacctctc attcgtatca
    17521 agctaccgat acgaataaac acaagttaag tgcgtatatt ctcaatggtt tcaaaaaata
    17581 caacgtaaaa tggtatttca agggaattca aaacagaaaa atttatacag gcatacgtgc
    17641 tgtaaatgaa tcaatttaca ttaaagacaa ttcttctgaa tttagcgatt gggatgtgtt
    17701 tgttcaggcg ccgggcgctg gaaacgagaa caagctatta atcgttgata aagcaatgaa
    17761 agatatagag aaaccgataa tactgtcaac atggtatttg gacgaagaaa acaatttgac
    17821 acaagatgga tcgaaagaaa atatcacgaa accaactgtc aatttggtta atatctacga
    17881 tcaaaccgca gaagataaag catcctacga agatgtggca aaatcagagt tgaaaggcaa
    17941 tacgtattcg catgaaatca aagtgaacgt tgtaagaaat gcaaaaaatt tgaatgtcga
    18001 aacgattgaa acagggatgt ttgccacgat ttcttataaa ggaaagatat acaagtcggt
    18061 tttgacagct tggcgaatat caagtgataa ggagtttgtg gaattgactt tcggaaacat
    18121 aagaagtcgt tttatggatt attttgaaga taatggggga taaaaaatgg ttagcaatgt
    18181 ggatggatat caatttgaaa acgtgaaagt aagcgcagaa aatgatgcta gactttatca
    18241 cgttttatat aatcggaaaa atcaggttat tgatggttac gatcagtcta tgaatttatc
    18301 ttcaagcgga ttaacagtaa aagttgctgc aggagcagcg attattcaag gtcgtatggt
    18361 cgttgttcga caagaagaaa gtataacagt tccagcaaac tcaagtggtt atatagcatt
    18421 aacagtggat ttgacacaag aagttatacc tggatctatt cttccagaat cggaagaata
    18481 tgaatggact aataatcaag tcaagctaga atttataaca aaagttataa aaggtaattt
    18541 aaacaatggt gataaggtct ataatttacc actatgctca gttaattcta ctggatcaac
    18601 tgtttcaatc tcaaagatat cggatagtta cgagctgact ctctctaaag gagaaatttt
    18661 gtggaggggg actgcgttaa tgcatgatac tcaaactgtc caaccttcaa aaaaaatttg
    18721 gcagacagtt tcaggttttt tgttagtatg gctcccatac gaaaacgggc aagcaattga
    18781 ggatagatat gtaactacgc ctttttataa ggagcgtgta acttttacta atgttttagg
    18841 agaaattgtt tcaggatttg atgactatca caaaaagtgg tttagtaaac gaataaacta
    18901 taattcgaat actaatatat ttacaggtgc agcaagcaat gcaagtggag ataacgcaaa
    18961 tatggtgctt aggtatatag tttcttttta gttaggaggt atggaagagt ggcaaattta
    19021 gaaattaaat tatctgcaaa taagagacag ccttatctac gtcaccgtgt tgttggtaga
    19081 gttggtgatg gggggctcac aacaatcaat gtacaacttc ttgaggaaga tgaaattaca
    19141 ccttttgtaa ttaatctaaa cggtactttg aaatttgtgg gcgaagtttc aaacggtaac
    19201 tataccgagg gagaaccaga aataatcgat tcgactaatg ggttaattag ttacacgttc
    19261 actaagtcaa atttcagcac gagtcatcaa ttcaaacaag catattttga atatgtagat
    19321 cctaacggca aaaaagtaac ttttcagaac ttcatcatag acgtgttaga acgagtggat
    19381 attaattcgg agcaagcgaa atactatatt tcttcattgg aaaaattaca gagtgaaatg
    19441 caaaccactt tcaatcagtt cattagtgat aaacaggtcc aatacgatca aatctactcg
    19501 aaatataacg aattagtaag attgataaat gaatcagata agcaagtaaa tgatcgtatt
    19561 gatcaaacca atcagcaaat cggcgatctc ggcaagctga aaaagatgta cagtaacagc
    19621 atcgactttg ggggctatga ttattcgggg aatccgaatt tgttaagtaa gctatcatac
    19681 gacttaattg aaaatcaaaa tacttcagct ggaacacttt ctaagggtga aaactcgttt
    19741 aaatataata agatatcagc tgaagtggaa ggtggagtag agttatatta taaacgaaga
    19801 ggtatagcta actggttacc ctctaataaa acgcttgtaa tgactgttaa gcttagagct
    19861 ggagcggact atagtccagt tgacggaaaa cttatactga ttagatatag gtatgttgac
    19921 agtggaactg gcaagattgt tttagactta cctattaaca gtaattcgat aactcaggaa
    19981 tggaaagagt ttagtattac tggaactact ccaacattta gcccacaagc ataccatcct
    20041 tggatacaat ttagggctca agatggggta cttggggaaa tagaaatgag ctatgacatc
    20101 aaaatcgaag aaggctctac agctacacca tatcagccta acttacttga tgactcttac
    20161 tggctaggta aaacaccgtt gggtgagaat ttgataacga atgccaagtt cccaattata
    20221 acacgaagca atcctatttc tggttttgat atttcagaag aattaataat aggtgaaact
    20281 tatacggtat ctttaaaagg gactaagcca gctactaaag aattccactt atactacgga
    20341 gaagcaaatt accagcagtt gcagtatcag gcaactttac ttcctgtgga aggattaaca
    20401 gatgtgtgga gtgccacttt tacagccaga aatactagtg aaacgacaaa tttacttaga
    20461 gtagcgttat ggcaaaaacc taattctgca acatatgata ctgttcaaat tgactggctc
    20521 aagattgaaa aaggcgacac acgcacacca aatatcagtc agtttaaata ctttggtgaa
    20581 ggcttgaaag acagtaacaa tccgaacgac tacagctggg atgtcacacc agagtatact
    20641 gaaaaaagct tgaataatac ggttagtctg actgaaccgc aatccatcga aggattgaaa
    20701 aatttcgaag atggtattca atcaaaagga aaatctgtat tgacatcaga cgacaacaaa
    20761 tatgaagtcg taaccttaac agttacaaac gggaataccg gatcagcaaa gctttatcgt
    20821 gaaggaaaaa ccgtcactat ttattttttt gcgttgaatg ggaaaagcag tggtggaaat
    20881 gattcaacga tactaacaat tccagaaggc tatcggccac caattagttt tgagcaactg
    20941 gttggctcga tagaccgttc tactttgaac agtgctcagt tatctattgg tgcagatgga
    21001 gccattaaat ggcgaagaaa ctcaagttat ggatcggatt atacctttgc aattacttac
    21061 acgatttaga aaggcgtgaa tcgatatgaa ggcagcatat agaccaattg aaccttacgg
    21121 attcgagcaa atcattgtga atgatgaaga acatttaccg gaagaatgca cagaagtcga
    21181 accaccgatt ccaaattgga aaccgaaatt caattactgg gagggaaata aatgaaaaac
    21241 atttggaaat atggccgtac tggcggagag tatgcaggaa aagtattgga cgacatgctt
    21301 gtatccgttc cttacacaga tcagccaccg ctcgaagggg ttcgttcaga tggcgaaccg
    21361 ctaacgattg ctgatcagat gtttgatcct aaattgaacc aatggattat tttagcgaac
    21421 gcgttagatc acaacgattt aaacaatctc aaagcgatgt acgaggctct tgaacatgaa
    21481 aacggcaacc taaaacagct aaatgccaaa ctcatgctaa gcgatgtagc gattaaacag
    21541 gaaaatactg cattgaaaga aaaagctgac agtttagcac aaatcaattc aaaaatgatg
    21601 cttgcttcgt tacaaaatag caaagacatt tcagaaatta aagagcaact aaatccagct
    21661 tcaaagggag gtgagtagta tgtttagttt tagcgatgtg aaaatgatgt atgattgggg
    21721 ctgttttact gacgatcaag ttcgtctatt cgttccacta tgtatcacag atgaagaagc
    21781 agatagaatt attagcaaag aagagagcgc atcttaagtg atgcgttttt ttgttggaaa
    21841 gttggtggaa catgaaagaa gaagcgctcc aagacgttgt ggagagatta gtaagaattg
    21901 aaacaaaatt agacaactac gaatcactta gagaaaaagc tgatagtgca aaagatttgg
    21961 cagacaaagc ctactcagta gcgctaaaca acgcagaaga tatcaaagaa atgaaagcca
    22021 ataataaatg gtcgtggggt tacatgatcg gtttaggcat tacgatcatt ggctatttct
    22081 tgactaaatt gtaaaggagt taagaagaaa tgattttacc cgataagtat tatcaagtca
    22141 ttaaatggac ggttttaaca gtattaccag ctgcatctgt tttagtagcc acgttaggaa
    22201 aagcgtatgg atggaatgga acagatatga cagtactcac tatcaatgca gtagcaacat
    22261 ttttaggcgt tatcactggt gtgtctgcat ataacttaaa aaaataggag gaaaaaaatg
    22321 aagaagaaaa tcattttatc attgagccta ataatggctc tttttttatt gccaattaac
    22381 gggttcgcct atacgatcaa caatgaattt aatttaggtg taaatgaagg tagctc
//
LOCUS       DACDIG010000046        22252 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.046, whole genome shotgun sequence.
ACCESSION   DACDIG010000046 DACDIG010000000
VERSION     DACDIG010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.046"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(159..746)
                     /locus_tag="IV247_001813"
     CDS             complement(159..746)
                     /locus_tag="IV247_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777881.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            921..2243
                     /locus_tag="IV247_001814"
     CDS             921..2243
                     /locus_tag="IV247_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8777882.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            2398..3174
                     /locus_tag="IV247_001815"
     CDS             2398..3174
                     /locus_tag="IV247_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP8777883.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            3167..3736
                     /gene="rnmV"
                     /locus_tag="IV247_001816"
     CDS             3167..3736
                     /gene="rnmV"
                     /locus_tag="IV247_001816"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAP8777884.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            3729..4613
                     /gene="rsmA"
                     /locus_tag="IV247_001817"
     CDS             3729..4613
                     /gene="rsmA"
                     /locus_tag="IV247_001817"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAP8777885.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            5064..5693
                     /locus_tag="IV247_001818"
     CDS             5064..5693
                     /locus_tag="IV247_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777886.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFGLIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(5817..6926)
                     /gene="ugpC"
                     /locus_tag="IV247_001819"
     CDS             complement(5817..6926)
                     /gene="ugpC"
                     /locus_tag="IV247_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAP8777887.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            7135..7557
                     /gene="mgsA"
                     /locus_tag="IV247_001820"
     CDS             7135..7557
                     /gene="mgsA"
                     /locus_tag="IV247_001820"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAP8777888.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            7880..8416
                     /locus_tag="IV247_001821"
     CDS             7880..8416
                     /locus_tag="IV247_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP8777889.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(8456..10219)
                     /locus_tag="IV247_001822"
     CDS             complement(8456..10219)
                     /locus_tag="IV247_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8777890.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(10222..11937)
                     /locus_tag="IV247_001823"
     CDS             complement(10222..11937)
                     /locus_tag="IV247_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8777891.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(12716..12940)
                     /locus_tag="IV247_001824"
     CDS             complement(12716..12940)
                     /locus_tag="IV247_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777892.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            13140..15908
                     /locus_tag="IV247_001825"
     CDS             13140..15908
                     /locus_tag="IV247_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8777893.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            15989..16408
                     /locus_tag="IV247_001826"
     CDS             15989..16408
                     /locus_tag="IV247_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAP8777894.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            16420..16890
                     /locus_tag="IV247_001827"
     CDS             16420..16890
                     /locus_tag="IV247_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8777895.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            16907..17686
                     /locus_tag="IV247_001828"
     CDS             16907..17686
                     /locus_tag="IV247_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8777896.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            17673..18494
                     /locus_tag="IV247_001829"
     CDS             17673..18494
                     /locus_tag="IV247_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8777897.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            18511..19560
                     /locus_tag="IV247_001830"
     CDS             18511..19560
                     /locus_tag="IV247_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8777898.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            19573..20577
                     /locus_tag="IV247_001831"
     CDS             19573..20577
                     /locus_tag="IV247_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8777899.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            21210..22229
                     /locus_tag="IV247_001832"
     CDS             21210..22229
                     /locus_tag="IV247_001832"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP8777900.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         7186 a         3896 c         4573 g         6597 t
ORIGIN      
        1 atttatatct aacagtagcg ttgatgtgtt aattataagt tctatcactt tatgttcaat
       61 tagtttgtgc tcttatatgt gtgattgaaa atatagataa aaaaacaatc atcggctttg
      121 acttcgatga ttgttttctt tattttgtaa aaatcctatc acttgatttt atggtaacct
      181 tttatttttg tcttcaaacg gcttggtaag agagcagtaa gcagtgcaaa tgggatcgaa
      241 aagacatacc agaaaatcgt acgtccgata ccataatgtt gatagagctt tgtcaacatc
      301 acgtgtaaca agatcaaccc taaaagcaag gagccagcta taaaccaatg attatcccaa
      361 tgcatgactt cactaagtgc catcactcca aaaggataga aataaaaata gatcccgact
      421 aaggtcatcc gccagttctt tgtaaatgtt ccgatattca ataaaaagaa aatcgctgaa
      481 agcaaaatac cgataggcgg aaaaagccca accaaaaagg aatacaacat cccccacaag
      541 atcatcacag gacctttgat caatgcaacg attgcgatga acaaagtcag caatattagt
      601 ttttgtgata acgttgcaac agtcattaat ccaaataaaa ttaatgtgaa ccaatgccaa
      661 tgggactgct gttgttcttt acgggaaaaa tcgacccggt tggataaata atctgctaat
      721 ttcggataag aacgcttaaa atccataaac tgttcctcct tcgtggaaaa ggtaggttta
      781 gtatacgaaa aataaaaaag aaatacatcc gtcttaagag ggagtttttc actgaaagtg
      841 gagaaaaaag aatggtaaat taaaatattc tcagttatac tgaaaattat accaaatttc
      901 aaagaagaaa gaggatggga atgaaaggca aactaaaacg agaaatcaat ctgtttggcg
      961 cattagctac cgttatggga accgtgatcg gtgcaggcgt cttcttcaaa actgctgctg
     1021 ttactgctag tactcagtct acaagtttga cattgctagc ttggcttttg ggtggtttct
     1081 taacgatctg tgctggattg acagtggctg aattagctac agcaatcccg gaaactggtg
     1141 gagctgtaaa atatattgaa gctgcgtatg gaaaattgcc cagcttttta ttaggatggg
     1201 cgcaaagctt gatttacttt cctgctaata ttgctgcatt atcgattatc tttgcgacac
     1261 aattgacgaa cttattacaa ttatctaccg attatctttt gttgattgca gtgatcaccg
     1321 ctgtttctgt gacagggttg aatttgttag ggacaaaagt cggtgcttct gtacaatcag
     1381 tgacgttgat tgttaaatta ataccaattg ctgtgattgt catatgggga ttattgaccc
     1441 caggacaagg aactgtccag ttatttccaa tcgaagctgg aaaagatgtt acttttgtcg
     1501 aaggattgag cagtgcgttg ttagcaacgt tgtttgctta tgacggctgg ttaggtgtcg
     1561 gtgcaatggc aggggaaatg aaacggccgg aaaaagattt gcctaaagca attattcttg
     1621 gtttgagttt tgtaacagtc gtttatctat tgatcaattt tgtttttttg aaaactttgc
     1681 cgattgatca tcttgccggt aatttgaatg cagcttctga agcatcagac gtcatttttg
     1741 gaggaatcgg tggaaagctg gtcactattg ggatcttgat ctcggtatac ggtgcattga
     1801 acggttatac actaactggg atccgcgtgc cttatgcgat ggctttagaa gacgagctac
     1861 ctttcagtaa acagctgaca aagctttcaa agaagtttac ggttccttat gttcctgcgg
     1921 ttttccagtt agcagtagct tgtattatga tgagccttgg ttctttcgac tttttaacag
     1981 acatgttgat ttttgtgatg tggctattca gtttactgat ttttatcggt gtctttattt
     2041 taagaaaaaa agcgccggaa ttgccacgtc catataaagt accactttat ccaatcgttc
     2101 cgattattgc gattctcggt gctattttca ttttaggtat gacgatgatg acgcaaacga
     2161 aattagcatt gatcggtata ggtgttacct tgataggtat cccagtctat tatcaaaaaa
     2221 agaaaaaacg ctcagaagag taaaatattt caacaacaga cagaaaaagt acgaactagg
     2281 tcctctagat cttcgtgctt tttctatttc aataaaacaa gttcttctat tttgattttt
     2341 gctacttctg caagtagtgc ttatgtgcta aagtaagaat gaaaaggaga gataaacatg
     2401 atatttgatt ctcataccca tttaaatgct gaacagttca atgaagatat tccagaaacg
     2461 attcagcgag cacaagaaat gggggtaacg aaaatggcgg tcgtgggctt cgatacgcca
     2521 acgattgaaa agtctttgca attaagccat gactatccaa atatatacag tattatcggc
     2581 tggcatccga cagaggctgg cagttatacg aaagaaatcg aaaataaatt acaagaacaa
     2641 ttgactttac caaaggttgt agcattaggg gaaattggct tggattatca ttggatggaa
     2701 gacccgaaag aagtccaaga taaagtattt cgcagacaga ttgctatcgc aaaagaaatg
     2761 aacctaccaa tcagtattca tactagagaa gcacttgaag acacgtatca aatcctaaaa
     2821 gaagaagatg ttcgagatat cggtggaatc atgcatagtt tcagtggtga ttatgaatgg
     2881 gcaaaacgat ttcttgactt agggatgcat atttcattta gcggtgtcgt cactttcaaa
     2941 aaagcattag acgtccaaga ggctgcagca aatgtaccaa tcgaacgatt acttgtggaa
     3001 acagatgccc cttatctggc accagttcct tttagaggaa agcgaaatga accaggctat
     3061 actcgttata cagtagaaaa gattgctgaa cttcggaatc ttccgacaga agaagtggct
     3121 gcccaaacat ggacaaatgc gcaccgatta tttggtctaa gcgaacatgg ataagatgaa
     3181 gatccaagag attatagtcg ttgaaggaaa agacgataca agaaggctgc gggaagtagt
     3241 tgatgcagat acgatagaaa caatagggtc tgcaatcaat gaagagatcc tgatgcaaat
     3301 tgaacatgcc caagagactc gtggagtgat cgtttttacc gatcctgatt tttcaggaga
     3361 aaaaataaga aaaatcatta tggaagctgt tcctcaggca aaacatgctt ttttgccgcg
     3421 cacacaagct aaaccaaaga aaaagggaag ccttggtgtc gagcatgcaa gtgatgctgc
     3481 tattttagaa gcattgaaaa aggtcgtgac gccagacatt tcccaaacgg ctgtttctga
     3541 aatcactaga caagaccttt tagactatgg tttaatagct ggggcacaag caaaagaaaa
     3601 acgagaaaaa ctaggtgacg agttgcgcat cggttacacg aacggcaaac aattggaaaa
     3661 acgtttgaag atgtttcgta ttacgccgaa agaactcgcc caagcaatga aaatagtgga
     3721 ggaacaacat gagtgaatat cgtgaaattg cgacaccctc tagaacaaaa gagattttat
     3781 tgaaacatgg tttttcattt aaaaaaagtt taggacaaaa ttttctgact gaaccaaata
     3841 ttttaaggaa aatcgtagaa actgcgcaaa tagatgaaca tacgaacgtg atcgaagttg
     3901 gaccaggaat cggtgcattg acggaacaat tagccaagca tgcaaaacaa gtcgtagcat
     3961 ttgagatcga tgatcgtttg atccctgtat tggctgatac gatgcaaccc tatgacaatg
     4021 tgactattgt acatcaagat attttaaaga ccgatttgtc aacagctgtt agagaaacat
     4081 ttcatgagga gcttccgtta aaagtcgtag cgaatttacc ttactatatt acgacaccga
     4141 tcatgatgca ttttcttgaa tcagatttgg ttgtggatga actagtcgtc atgatgcaaa
     4201 aagaagtagc tgatcgtatc tctgctgaac caggaacgaa agcttatggt tcattatcga
     4261 ttgctgttca atattatatg gaagccagtt tagcattcat cgtaccaaaa acagtatttg
     4321 tccctcagcc aaatgtagat tcagcgattt tgaaactgac aagaagagat ataccggcag
     4381 tcgaagtgac agacgaaaaa gcatttttcc gattgacaaa agcggcattt cagcaaagaa
     4441 gaaaaacgct ttggaataac ttgcagcatt cgtatggcaa agatgaccag acaaaagcat
     4501 ggttagcaaa aagcttggaa acagctggaa tcgatccaaa acgtcgtggg gagactttgt
     4561 cattacaaga attcgccgca ttaagcaatg cgatgtcaga aaatatgcag taaaaaaaga
     4621 ggttgtgaca gaagttgtga aaaagctgtc ctgcatcaag taataagaac agccaaacaa
     4681 aaatagttcc tcatgttttt cgtttgtttg ggcttattac cgcagatgca agcttttgaa
     4741 caccgtttaa gaaagaggtt gtgggcctgc cgtttaactc taagtcacaa ggaacaattt
     4801 caaaaaacag cttctcatgt ttttgagtta gttggtctta ttacttgatg caagcttttg
     4861 accactatta tgccgaacaa ggagatgtga cacaaatttt gtcacatctc ctatttttgt
     4921 ttcttgatca aaaaattctt atacttgata ctcacactca ttttgtatgg atttagaaaa
     4981 gaatatgtgt cttgacagtt attagtaagt gagttatact aagtcgacat ttatgtgtca
     5041 agacatatta ggagagagaa aaaatgagaa aaaattcagt tcgaaaacta acgatttctg
     5101 ctttattgat tgggatgggg gtcattatcc caatggtcat gcctaaaatc atgattccac
     5161 cagcttcctt tactttggcg tctcatgtgc cattatttat tgcgatgttt tttacaccag
     5221 gagttgcagt ggctgttgcg ctaggtacga catttggttt ctttttaaca acgccggtta
     5281 tcatcgcatt gcgagcattg tcacatcttg tgtttgggtt gattggggct tggtacttgc
     5341 aaaagcatcc gagtatcgtt ttaaaaaatg gacagtttac ttttgctaat tggcgtttcc
     5401 aagggttcaa ttttgtgatc ggtgtgattc attctttagc agaaatgcta gtagtcagca
     5461 tcttttactt tattggtaat atgcctgaaa catattattc tcaaggatat ttttatacag
     5521 tattcatctt gatggggatt ggtgggctta tccacagttt agttgattat aatattgctt
     5581 attttatagc aggaacactc agcaaatatt ttgatatccc cgtatttacg gcagcaaaaa
     5641 aagaaaatcg gaaagagtcg attcaagggt acgcgaagaa aacgacatat tagacatgat
     5701 ctctcaaaaa aaaaactctc atctagtaat ggataataaa tcctctaaga tgagagtttt
     5761 ttgtcctaaa aagagctgca ccaacatctt ctggcacagc tctaacaggg ggcaatttat
     5821 ggaagtttga tgacttgttc tgtttcttta tcaaagaagt ggcctttgct gatgttgaag
     5881 gcaaggtcga ctatttcgcc tggtttatgg aaatctcttg catctacttt tgagatgaac
     5941 tcagtagaac cagtttttgt gtaaagcatc gtttcagctc caagtaattc ggaaacgaca
     6001 acttctgatt tcactacggc ttcaggtgaa gcttcaaggg ctacttgttc actgtggatg
     6061 tcttctggac gaataccgaa gataacttct tttccttcat aacctttttc aactaatact
     6121 ttgttttttc cttcaggaat acgaagctta agcccatgtc catctgtgat aacaccattt
     6181 ttcaatgtca cattaaagaa gttcatggca ggagatccaa taaagccggc aacgaacata
     6241 ttgacgggtg tatcataaac ttctttcggt gtaccgattt gttggataaa tccgtctttc
     6301 atgataacga ttcggtcagc catagtcatt gcttctgtct gatcgtgagt aacatagatg
     6361 gtcgttgttt caagacgctg gtgaagtttg gcaatctcag cacgcatggc tacacgtaat
     6421 ttagcatcta aatttgataa tggctcatcc attaagaaga cttttgcatc ccgaacgatt
     6481 gcccgtccca atgccacacg ttggcgttgt ccaccagata aagcagctgg ttttcgtttc
     6541 aagtattctg tcagacctaa gatctctgct gcattgtcca cacgtttttt gatgtctgct
     6601 ttatcatatt tacgtaattt caagccaaaa gccatattat caaaaacagt catatgagga
     6661 tacaatgcgt agttttggaa aaccatggca atatcacggt ctttaggtgc cacgtcattc
     6721 atgacttttc cgccgatatt tagttctcct tcagagatat cttccagtcc cgcgatcatt
     6781 cgcaaagtag ttgactttcc acaaccggaa ggaccaacaa aaacgataaa ttcacggtct
     6841 ttgattgata gattaaaatc agttacagaa taattttctg cattgtcata ttttttatat
     6901 acgtttttca gagccatttc taccataata cgttccacac tttctttttt agttttatta
     6961 actatttaaa gtataaatga aagcgttaac taacttcaat gtcatgttga acaagaattt
     7021 tttctttttt ttgtcaatgt gctagtttta agctttaata aatatccata agaaataaaa
     7081 actgatacac tggagtaaat aagtatgtta acaatatcaa aaggagtgtc tcttatgaaa
     7141 atcgctttga tcgctcatga ccgaaaaaaa cctatgatgg tgaaattagc aacagcgtat
     7201 aaaacaatat tggaaaaaca tgaacttttt gctaccggga cgactgggca gaggatcacg
     7261 gaagcaaccg gcttgccgat ccatcggttt aaatccggac cgttaggcgg ggatcagcaa
     7321 attggagcca tgatttcgga ggatcagtta gatttggtga tttttcttcg agacccgctg
     7381 gctgcacagc cccatgaacc agatgtaaca gcacttatcc gtttatctga tgtctatgag
     7441 attccgttag ctacgaatat cggaacagct gaggtgctac taagaggact ggaagccggc
     7501 ttcctagatt ttcgaagaat cgtccatgaa atagacaagc atccactttc attttgatgg
     7561 ataagcgtat ggaaaaataa cgtttaatgt tcgcctatat gtagctataa aagctaaaca
     7621 aactgataat atgcgatata atacgaacgt attggataat ataaacaata atattagttt
     7681 ttcgtatttg cagtaaatgt gaaaatatgc tatagtttct gtgggaaaag atcccaaaaa
     7741 taatacagag tagaaaataa agcgttaagt gctaagagga tgggatgttg cctcttggac
     7801 gaaggacgta atgttcgcgg ttttattttc gcattcgctg catgatacga tgtagcagct
     7861 cttaaaggag atgctaaaaa tgaagaaaca ccggttagac gcacgtatga ttgctattat
     7921 gggcttattg attgcattga tggtcacttt gtcccgactg gtagcaatcg agaccccatt
     7981 tattaaaatc agtgtcacat ttattccaca agtgatcatg ggcattcttt ttggtccttt
     8041 ttggtcaggg ataggcgcag ttttagctga tttagtagga atggccctct tctcgaaatc
     8101 tgcattcttt attggattta cattgaatgc atttattgaa ggagcaatct acggattctt
     8161 tttctatcga aaagagatca cttggaaaaa tgccattctg gctacgctta gtgtcacgct
     8221 gatcatcaac ttatttttga caccattatg gcttgcgttg atgtaccatg tgccattgtt
     8281 cagctgggtc gtttgggcgc cccgcctgtt aaaaacagta atatggttac cgattcaatc
     8341 aattgccatt tattacgtag gacgtagtat tccttacaaa aaaattttaa gaagtttagc
     8401 gatccacgct aaataaaaaa caagcagtct tcccatccca gaagactgct tgtttttatt
     8461 gtgctgtgaa ttgtgcttga tagagccgtc ggtaataacc atctttttga agcagctcat
     8521 catgggtgcc catttcgaca atagctccct gatccatcac taatatctgg tccgcatttt
     8581 tgattgtgga caggcgatgg gcaatcacaa aactggtttt tccttccatc attgctaaga
     8641 aggcttgttg gatttttttc tctgtcaatg tatcgactga gctggtagct tcatctaaga
     8701 tcaacatagg gggattactg atcatcgttc gagcaatcgt caacagttgg cgttgtccat
     8761 ctgatatctt taacccttga ccgccaatct tagtatctaa tttttccggc aaacgttcta
     8821 caaattcata catataagag gcttttaggg catcattgat ttctgcgtca cttgcttccg
     8881 gattcccaaa acgaagattt tctcgcaatg tactgtcaaa tagccaagta tcttgtaaca
     8941 ccattccaaa acttttacgt aatgtatcac gttggataga gcggatgtca tggtcatcga
     9001 ctgtaattgt tccttttgtt gtgtcataaa aacgcatcag caaattaaca agtgtggatt
     9061 ttccggcccc tgtttttcct actatagcaa tggtttgacc tggataagca gtaaaattga
     9121 aatcttcgat caaacgctgc ttggggtcat aagaaaaaga aacatgatca aaagagactt
     9181 ttcctttgat atttttgggt tccaaggcat aggcatctgg caaatcaggc ttttcttctg
     9241 gctgatcgat caaatcaaat gctcgttcta gaccagaaaa agcggtttgc agttgtgtcg
     9301 tgattccaga aagttcgata aatggtttag aaaactgact tgagtagatc gtaaagctgg
     9361 aaatcacacc aacagtgatt gacgaaccac tcttcaatgc caataggccg ccaactaaac
     9421 caatcgataa ataagcaagg tgatctacaa aacgagaaga cgggtttgtc aaggaagaag
     9481 aaaattgtgc ccgttgtcct ttttcatata gatcagcatt tagcagttca aattgctgtt
     9541 gactagcttc ttcttgctga aaagctttca cgattttctg gttaccgatt cgttcggtca
     9601 caaagcccga gatatcccca ataatccttt gctgtctatc gaaatttgtt tgtgaagcac
     9661 gagcaacgat ccaactcaca aggaaaataa ttggtgtaga acaaagaacg accaatgtca
     9721 gaagtggact tagacgcatc ataaaaataa acgaaagaat gatgattgcc actccagaaa
     9781 ataattgatt gaaggccgca gaaattgcaa tggaaatatt gtccatatca tttgtaaatc
     9841 gactgacgat atttccgtgg gagttttgat cataataacg aagtggcaat cgattcagat
     9901 gagcaaatgc atcttttctt aattttgcaa cggataagta agctaaccga ttgcttaaca
     9961 cttggatgag ccattgactg actacgttga ttaaaacgat accaccgaat agaataagta
    10021 aatggaacag tcgattgaag cggacctgtc cttctccaat catttgatcg atactttgac
    10081 caatttgtaa cgtcatcaca actgttgcaa tcccattgat gattcctagt atcacagcta
    10141 gccaaatgtc ttttgcatag gcagtaaggt aaggaatgaa tcggcggagc gtctgcaagg
    10201 ataatttttt tactttcata actattcttc ctcctgtgat gcgacgattt cctgatattc
    10261 tgctgattga gcaagtaatt cttcatgggt accctgagca gcaatcttac cttgatccat
    10321 cactaagata tgatgtgctt cttgaataga gcggacacgt tgggagataa taatcactgt
    10381 actgccaaga taagtctgta gcgccttacg caaatccaag tctgtttgat aatccaacgc
    10441 acttaaagag tcatctaaaa tcagtaatgc tggcttggca atcaaagctc ttgcaatcgt
    10501 cagtcgctgt ttttgtccgc cagagaaatt tttcccattt tccatgactt ttgtatctaa
    10561 accatttgga agctgacgga caaattcttc tgcctgagcg attttcagag cttcccaaca
    10621 ttctttttct gtggcgtctt tcttacccca ttgaaggttt tcacgaatag tcccggtaaa
    10681 tagtacagaa gtttggggaa caagagcaat ttgctgacgg agatgatcca gctgccaaga
    10741 acgaacatcc attccgtcca ttataactgt accggaactg acatcataga aacgcggaat
    10801 caattgtgtc aaagtcgttt tcccgcttcc ggttggacca atgattccaa ggactgtatt
    10861 tcgcttcagc tgaaatgtaa tattttggag agaaagacca gaatcaggag tataacggaa
    10921 gtcaacatgg tcaaaagcga taattgcttc agaagaaaaa tcgggagtag tactgacctc
    10981 agcttcaaga atcgaatttt ctgtgtctag cacctctttc acccgatttc ctgaagcttc
    11041 ggcacgtgtg aagatcacga cgaggtttga aacaacgatc agtgccaaaa gcatttgatt
    11101 catataattg atcaaagcaa gaacttgtcc ttgttgcaag ctaccaatat ttactttgat
    11161 cccgccaact gtcagcaaac agatgatccc gacattcatg atcaaggttg tagcaggtga
    11221 taatagggca gagatattgg aaacacgtaa ataattgttc gctagatcat ttgtgctttt
    11281 atcgaacttc tctgtttctt tttttgtctg cgcaaatgcg cggatgacac gaacaccgct
    11341 aagattctga ctaacgtgtt cattgagctg atctagtttt tcttgcactt tttggtagag
    11401 aggaacggtt tttttgatga taaaaaataa aatgattgag aaaataggca ataatgctaa
    11461 gaaaaacaga cctacttccc agtcgatcac aaaagccatg accactgacc cgatacttaa
    11521 aaacggtgct ctgatcaata aacgaatgac cattgctaaa gctagctgaa gctgattgat
    11581 gtcgtttgtc attcgggtga tcaaggtatc tgttccaaaa tgattcaact cgttgtgaga
    11641 gaaagtattg atttttttga ttagctgatt acgtaactct gtaccaaatc cttgtgaagc
    11701 tactgaagca tagtattggc aaatgatagc acaaatcaga ccaatcacag acatagcgac
    11761 catccataag cccatttcaa tgatttttcc tcggtcattc atttttagtc cattatcgat
    11821 caaggaagcc atcattaaag gcaggacaag ttcgaagaca gcttcaagaa atttgaagaa
    11881 cgggcccaaa atgatttgct tacgatagtc ttttgcataa cgtaataact gaatcatatg
    11941 attcctccta ttatttattt gtccacaatt gaaaaaacaa gtactaccac attgtagctt
    12001 aggaaattta gaatgaaaag agaacaagcc ttttcataaa tgaaagtttt attatgaaga
    12061 agctttagaa attgaataga ctattttttt taaaaaaaag aaaatgagaa aacatgccag
    12121 tctcttcatt cataggaacg tctattttac ttaatttttc tataaatgaa ttctgcataa
    12181 aaaatatatc aagtaaatgt ttatagatga aactctattt acttcttttt taatttgttc
    12241 ttgcaatctt ttttgctacg taatttttgt aaaaaagtgg gttgagagaa atctaattga
    12301 ttttcaaaga aggagtatga aaatgagaaa aatatttatt cgtttattgt gggtactatc
    12361 ttagaggaat aaataatagt ggagaaaact atggtttggc gcagattcta tcaggtttga
    12421 tggaacgaag acaacctccc gacatcaagt caaaagctac ttgagatttt gatctgaaaa
    12481 taaaaaaaga aaacaaggta ggaacatcaa tgtttctacc ttgttttcgt aatcaatggg
    12541 aaaaaactaa gagtggtgta cgtaatcaat aaaaaggatg gtgacaacag tacctcttta
    12601 ctctctgaaa gcatcataac tcgtttttcc tcattatgcc aataaattgt tgttatacca
    12661 catataagga tttgtaatag ctaaaaacag acaggaaagt tgaaatctgt gaatcttatt
    12721 ttgtatggaa ggatttatag aacgcaaaaa tcaagataaa gatgaataaa aatcctgttt
    12781 cggctatata ttgcgtatct acgccaataa aacgagtttg ttctgtcata gccatataaa
    12841 gcaaaggcaa aaatacaact acagcgagat aagatccgat gatttctcga aaagttttcc
    12901 aggaagtatg tttcctgttt ttatatttat ccgatttcat tgaatagtca tagcctcctt
    12961 attctttcat atgttgaaat tccagtataa aaactggaag gaactgtacg ttttttagaa
    13021 taaaaattat aagggataag taggctgctg tctataataa gatatatttt tcaattcttt
    13081 attaaagatt ttattgacaa aatgaatccg ttttctatga tggaggaggg gtgaataagt
    13141 tgttgaagaa ggaaatttta tgttacttag agaaccagac agcctttttc gatttagaac
    13201 atatgaacga ggtctttaca gcacaaaggt tagctgatca ttttggggta aaaagaaaca
    13261 cagtcagtca ttatttgaac cagttaaatg atgagcaact actagtcaaa atcaattctc
    13321 gaccagtgat ttatttccac aaagaagctt ttgaaaaaca attttttaag ttacgcacga
    13381 atttttattt ttctatcgat gatctcaaag aggaacagcc ttttttcgcg caaccaaaag
    13441 acttattctc ttttatgatc ggatacgacg ccagtttgaa agaaagtatc gaacaaatca
    13501 aaacagctct ttactatcca gatggcggat taccactttt gattacaggc gaaagtggca
    13561 caggtaaaag ttatttagtt aatttggtct atcagtattg tctgcttcat gatctgctag
    13621 aagattctgc accattcatc acttttaatt gtgcacaata tgcagataat cctgaacttt
    13681 tgactagcaa tttgtttgga catgttaagg gagcgtacac tggtgcagaa gaaaataaaa
    13741 aaggggcttt tgaagcagca gatggtggga tcttgttttt agacgaggta catcgcttaa
    13801 caccagaagg tcaggaaaaa ctgttcacct atcttgatca aggtgtgatt tatcgtatgg
    13861 gagaacctaa tttgtcacgc cggatcaaaa cacgtctctt ttttgctacg actgaagaga
    13921 ttactagtca tttcttaaca actttcattc gaagaatacc tatccgaatc gaactacctg
    13981 cacttaatca gcgtagtcgt aatgaacggc tagaactgat ttactctttt tttctcgaag
    14041 aacaaagaaa aatggggagc cctttgtcag tcagtggacc agctttgtca cttttaacaa
    14101 gtcagaaatt atccggaaac ataggagagc tgaaaaatat cgtcaaagtt tcagcagcaa
    14161 aagcatatgc tgaacagcgt gaacagcctg aaatccatgt cacgatccat catttgcaaa
    14221 aagaactact tgcgcagcct attactaaaa atacagcaca gcaaggaatc tatattacgc
    14281 aagaccagac cttagagcaa ttaatggaga agcaacagcc cgaacagcag cgaatcgtta
    14341 aaagttttga gcaaatccta attgatttca agaaaaataa ctgtctgcta tattcttgcg
    14401 aaggaaaatt aaagcaagaa gtgattcaat tatttgattt tttgctgttt gagaccagta
    14461 gacaagaaaa acacgagctg ctggtttatt ggacacaatc tatccgagag acctttcgac
    14521 aaatggaaac tgcctatcaa atcaaattcg atggaaacag tgtctatgcc ttgagttact
    14581 atctctatca gcgaagcact atcaaatggt taccggaaga acatgatatt atccaactaa
    14641 tcaaagaatt agaacaacaa gtttctcagt cttatccatc tagttatcac tatgtccaac
    14701 gaattctgga attaagtaag atcaaattag atattgaaga cacaagtatg gatcggattt
    14761 tactgacgat ctatctaaaa aaagcgaaat ggtcaaaaga aaaacgactg ccaaaagcga
    14821 tgattgctgc tcacggttat gcaactgcta gcagtatggc aaatgtagta aatcgtttat
    14881 tacaagagga tctatttgaa tcatttgata tgccactaga tgtcacacca cagcagattg
    14941 cttcagaaat ccttgattat tgcgaaaaca gcgatgtttc aaatggattg atcatattag
    15001 tagacatggg atctctgaaa gaaatccatc agttttttaa aaaacagtta tccgtaccgc
    15061 ttttgatttt gaataatgtg acgacacctt tagcaatcac agtaggggaa tgcctgcaaa
    15121 aaaatgtatc attagaagag atagcagaag agactgttcg acagatccag ccagaatggc
    15181 gactgctcta tccagaggaa aacaaaccca aagcactcat tactacttgc ttcactggaa
    15241 tcggtacggc ggtccactta agtgaactgc tagaaaaaag cttacctacg gcttgtcagt
    15301 tgaaaatcat cccatatgaa tatcagcaat taaaagataa gaaaaacagt gacccattat
    15361 tttccatata cgaagtcgta ggaatgattg gcaccacaga tcctgatatc accaatatcg
    15421 actatctttc attagaagac ctcatctcgg gggaaaagat gtctgtacta gctgaatggt
    15481 tggacactac tatgaatgct tcagagaagg aaatattcaa tcagcgcatc attcgtaatt
    15541 tttcattgga aaaagtactg gattccgtga ctattctaga tacagagaag gtgatgggag
    15601 aaatcgatca ttttatgaga gaattagagg tgcagttgaa tcgaagcatc agcaatccca
    15661 aaaaattagc gttgtatgtc catgtgagct gtctcattga acgattgatc cgacaaatgc
    15721 caatcgaaac ctatcaagga ttagagaaat ggaagcagtg tcaaaaagaa gggttatctg
    15781 ctattaaatc tgcgtttagt gtcattgaag agcattatag tgtcatgatc cccaattctg
    15841 agattgccta tatctatgat attttgagtg aagatacaga gtttctctcg atagaagaag
    15901 aattttgaac ataagcaagt tgttgccact aattttggca tgcaacttgc ttttttagta
    15961 tgtgtaagtg aaaagaggag atgaaaaagt ggatagagaa attatcttag cttcccatgg
    16021 aaaatttgct tcagggatat tagattcatt agagctgatc tatgggaaaa atcattctat
    16081 tactgtattc gattgctata ctgatgagaa ttttgattta gcggaaacag taaggcagct
    16141 ttttatgagg tacaaagata aagaaatcat tgttctgacg gatctttttg gcggaagtgt
    16201 caacaacgaa ttcttacaat atatcaatcg ggatcatgtc tatctcgttg ccggattgaa
    16261 tttgccacta ttgattgagc tagtatctcg gttggacgct gaagtagaga caactgtttt
    16321 gatccagcag gtgctaactg attcaaaaaa aatgatccag ttttgtaacg agcggctcaa
    16381 tcagaaaata gaagaagacg aattttagga ggaagaaaaa tgattgtatt gacaagagtc
    16441 gaccatcggt tattacacgg acaagtagct ttttcatgga cacaaacaat aggcgcagac
    16501 tgtattttga ttgctaatga tgatgtacct acgaatgaga tacgaaagac aacgatcaaa
    16561 ttagccaagc cacaaggagt caaactagtg atcaaatcaa ttgatgactc gattgccgcg
    16621 ttgaaaagcg gggtgacaga taaatataag ttattcatcg tagtggaatc gatcgaggat
    16681 gcatataagc tggcagaagc gtatccagaa atcaaaacca tcaacttagg agggatcaag
    16741 gcaaaacctg gtactcgcag catcggaaaa gcggtaaata ttttaccaga agaggaggaa
    16801 cgcttgaaac gattaattga caaacaaata gaagtagaaa tccgtcaagt accagcagat
    16861 aaaaaagtgg atgtaagaga agttttgtaa gaaaggaaga acctcaatga ttgtacaagc
    16921 agtcttatta ggaattgttg catttgtcgc acaatcagaa tatgcactag ggacatcgtt
    16981 gctttctcgt ccgattgtga cggggctgtt tacaggaatc gtgttaggtg atgtaaaaac
    17041 tgggatcatt atgggcgcaa ctttagagtt agcatttatc ggatcgttct cagtaggtgc
    17101 ttcgattcca cctgatgttg tgaccggtgg gattttaggg acggcttttg ctattacagc
    17161 tggagccggt acagaaaccg cgcttttact aggtttgccg attgctacgt tgacattgat
    17221 attgaaaaat atttacttag ggcttttgat tccaattatg aaccataaag cagacaatta
    17281 tgcagaagaa ggaaattata aaggagtcga gcatatgcac ttgcttgccg gtatcggtct
    17341 ttccttgatg cttggtgttg tggtaatggt ttcttatatg gtgggaagca atacgatcgg
    17401 aaatctatta aatatgattc ctgacttcgt tcaaaagggg ttgtcagtag ctacagggtt
    17461 gattccagct cttggttttg cgatgcttgc tcgtttgctg atcaacaaac aagttgcacc
    17521 atattttttc ttaggttttg ctatagcttc ttacttagag attcctgtga ccggagtagc
    17581 tatttttggt gcgattttag cagttgtggt agtcaatatt atgaatgtcc gtcaactacc
    17641 acttcaaaca actgcagggg aggtagaaga agatgaagaa ttctgagatc aaaaacagca
    17701 aaataacgaa aaaagagcta aatcacgtat tttggcgttc ttttcaaatg gagttttctt
    17761 ggaattatga acgtcagatg aatatggctt atgtttatgc aatgattccg attttgaaaa
    17821 agttatatca gtcgaaagaa gaaatggcag cagctttgaa acggcatcta gagtttttta
    17881 acacgacccc acatatcgtt acactgattt taggaatcac ggctgcaatg gaggaagaaa
    17941 ataaggatga tcctgaattt gatgtgactg cgatcgatag tatcaaaaca tctttgatgg
    18001 gacctcttgc agggttagga gattctttct tttggggaac tttgcgattg attgccacag
    18061 gtgtgggtac ctctttagcc atgcaaggaa atattttagg ccctatcttg ttcattttga
    18121 ttttcaatat tccgcacatt ctgtttcgct atctagcaac aggttgggga tatcgcttag
    18181 gaacgggttt tttgaaaaag atccaggcaa acggtatgat ggaaagtttg acattaggcg
    18241 cgtctattat tggactgatg gtcgttggag caatgactgc cacgatgatc accatcaata
    18301 ttcctttaaa aatcggttca ggagaaaatg ctgtgactgt tcaaagtatt tttgatgata
    18361 tcgttccaaa tatcttatgt cttggagcat ttggcatagt gttttatcta ttgaagaaag
    18421 aagtgaaacc attaacgatc ttgatcggac tagctttctt tggaattttt ggttcgtgga
    18481 tagggatttt ttaaaaaagg agcagagaaa atgtttacga tgcaagatta tatctatgaa
    18541 gaaaaggaag tactaagtac gatcttgaaa aagaatgatt tttcgacaag agaacatatg
    18601 aaaaaaacag tgaatttact gatcttagca acgggttcat cttataacgc ttgtttagca
    18661 gcgaagccag cattggaatc ttatggtgat cttactgtcg atattcaaga acctttttat
    18721 tttttcaatt atggaaaact ttctccttca atcgatactg tgattgctgt gtcacaaagc
    18781 gggaaaagtg cttctactgt tgaagcagta aaaatgatac aaaaacaagg gttacctgtt
    18841 gttgctatca caaatgacgt gcaaagtcca cttgctttag aagctgcaca aattattgat
    18901 ttaggtgccg gggtagaatc agtaggcttc gttacaaaag gctattctgc gactgtttta
    18961 cagttactac tcttagggat aggaattgga ataagtaaaa ataaaatcag caaaaaaata
    19021 gaacaagact acatgcaaca gttgcgcaaa ataatcaacc atttaccagc gattattcaa
    19081 aaaacggaag aattttttga tggatatcaa agtttatttc gtttagcaca gcgtttcgtc
    19141 accattggat atggtccaaa ctggggaaca gctaaagaag cagagacgaa attgactgaa
    19201 acaatccgcg ttccgtcaca aggatttgag ttagaagctt atatgcacgg cccatacctg
    19261 gaggcagatg cttcgcattt gctattcttt attgaagggg aaagtgtaaa taaggaacgt
    19321 tcgcaaaagt tgcaaagata tatgagtcgg tacgttggag agactctcac cattaccaca
    19381 aaaaaagcaa gaaacgagaa aacacttgga ttagcaatag aatgtgatga atatctgtct
    19441 gtattagcgc ttgtcgttcc gtttcaacta ttcgcttata aaaccgcagt agctaaaggt
    19501 attgacttga acaaaaagat ttttgaagac tttgatacag tcttgaaaag taaattataa
    19561 gaggtggaaa tcatgctgaa attcaatgaa gaagaacaaa tcaaagcaat aaaaggcgca
    19621 ctagctttac gtccgcaagt agaagaaatc atcgataaac tttatgcgga aaaatttgac
    19681 gctgtctatt atctaggaat cggtggaacg tatgcttcct cgatgcaagc tgtcacttat
    19741 atgaatggga aaagcaactt gcctgtcttt gtacagcatg ctgcagaata ttacactact
    19801 ggaaacaaaa gattaacaaa agattctatc gttattttgt cctctgttac cggtacaact
    19861 caagaagtag tgaaagcagt agaacaaatc aagaaagtag gagcgacact gattggattt
    19921 atcgataaag cgaacagtaa gttgtctcag ctatgcgatt ttgtggtcac ttatccagct
    19981 cctggaacag aacaaatcaa attttttatg gcagcagatc gattgatgta tttgaatgga
    20041 gaattcgaag cttattcaga atactatgaa cagctcgaac gttatttacc aacagggtta
    20101 gtggaagcag agaaaaaagc agatgcattt ggcttatcat ttgcagaaaa acatcgacgt
    20161 gatagtatgc attattttat tggagcaggg aatcaatggg gagctgttta ttcatatgcc
    20221 atgtgttact gggaagaaca aagctggctt ccatcgaaat ctatccatgc tgccgagttt
    20281 cttcacggga cgttagagat cgtagaagaa acaaccccag tcacattatt tttaggcgaa
    20341 gatgaacaga gagtgttatc tgaacgtgtg gctaaacttt tgccaaaaat ttgcagcaat
    20401 tatacattga tagacacaaa agactatcca gtagaaggta tcagtgaaaa atatcgcgga
    20461 cgtgttttgt ctttcttatt gatgcatgta gtgacacaac gcatcgatgc tcatgtggag
    20521 cagttgaact gccatccatt agagatacgc cgctactatc gtcagtttga ttattaggat
    20581 agactggata aaaagaatat aaaaaataaa gagagcagcg ataactgctc cctttatttt
    20641 tttaaaatat tggcatcaat tacgttataa aacacatttt ttgcattatc tacagttcaa
    20701 cttataatct gtttaatggg acatgtgggt gccaaccatt ttttgttatg caatagtact
    20761 atttagttgt tgcatggatc cgtagtaaaa gtccaagtac aacgagttgc gccagtttac
    20821 atagcgtttt gtatccagaa tgttgactaa aacaaaagtt cctatgtcaa ccgtcaacag
    20881 agacaatctg tccgttgcag gacctgacag atttagaaat gtccaatctg tctcttatgt
    20941 attttaaatc aatactcact aggagaagtt tcctaacctc ctaatagtaa tgctggtagt
    21001 aacattgtca aaactgcttc ctcttaggaa caagcaaagc ataacgagtg taaaaataaa
    21061 atgtagacta ttgtaacata tcattgatta tttcaagtaa aaacgataaa agaagtaaac
    21121 aaacacttaa aaacaatgga aattagtccc aactttaaga aatgtatact ataatggaaa
    21181 ttatcaatta tttatttgga ggaatatcca tgaaacaaaa aatcgcagtt cttggtcctg
    21241 gatcttgggg cactgcttta gcacaaacat tggcagaaaa cgggcatgat gtccgtattt
    21301 ggggaaatgt acctgaacag attgatgaaa tcaataagta tcatacaaat cagcattttt
    21361 tacctgattt gacgatccca gaatcgatta tcggatataa agaattggca gaagcagtgg
    21421 ataacgcaga tgctatttta tttgttgttc caacaaaagc gattcgttca gtcgcacagg
    21481 aacttattgg taaaatgaat actaagccaa tcattataca tgcaagtaaa ggactagaac
    21541 aagatacgca taaacgaatc tcagaagttt tggctgaaga aattccagaa gaaaaaagac
    21601 aggcaattgt tgtattatct ggtcctagcc atgctgagga agtggcagta catgatatca
    21661 caacaattac agctgcaagt attgatcaaa aagcagcagc ctatgtacaa aaactgttca
    21721 tgaatgatta tttcaggatt tatacaaata atgatgtgat tggcgtagaa actggtgcag
    21781 cactgaaaaa tatcattgca attggtgccg gcgctatcca tggattagga ttcggagatg
    21841 atgcaaaagc agcaatcatg actcgaggat tggcagaaat cagccgtctt ggtgtcgcga
    21901 tgggtgccaa tcctctgaca tttatcggtt tgagtggtgt gggggacttg atcgttactt
    21961 gtacaagtgt tcattctcgc aattggcgtg ccggtaactt gttaggacag ggacataagc
    22021 tggaagaagt tctagaaaac atgggaatgg tcgttgaggg tgtggcaacg acaaaagcag
    22081 cagtagagct agctcaacag ctaggtgttg agatgccaat tactcaaaca atctataatg
    22141 ttttgtataa tggagaagat atcaaaaaag cagcaaaaga aatcatgtta cgtgacggaa
    22201 aaatggagaa tgaattttct cttcgttaaa tagatgatca atagttaatc gt
//
LOCUS       DACDIG010000047        22165 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.047, whole genome shotgun sequence.
ACCESSION   DACDIG010000047 DACDIG010000000
VERSION     DACDIG010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.047"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            235..2070
                     /gene="tdc"
                     /locus_tag="IV247_001833"
     CDS             235..2070
                     /gene="tdc"
                     /locus_tag="IV247_001833"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP8777901.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2375..3853
                     /locus_tag="IV247_001834"
     CDS             2375..3853
                     /locus_tag="IV247_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8777902.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3819..5150
                     /locus_tag="IV247_001835"
     CDS             3819..5150
                     /locus_tag="IV247_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8777903.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5264..7816
                     /locus_tag="IV247_001836"
     CDS             5264..7816
                     /locus_tag="IV247_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAP8777904.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7823..9262)
                     /locus_tag="IV247_001837"
     CDS             complement(7823..9262)
                     /locus_tag="IV247_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8777905.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9608..9970
                     /locus_tag="IV247_001838"
     CDS             9608..9970
                     /locus_tag="IV247_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777906.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(10157..11014)
                     /locus_tag="IV247_001839"
     CDS             complement(10157..11014)
                     /locus_tag="IV247_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8777907.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            11134..12045
                     /locus_tag="IV247_001840"
     CDS             11134..12045
                     /locus_tag="IV247_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP8777908.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            12052..13698
                     /gene="mdcA"
                     /locus_tag="IV247_001841"
     CDS             12052..13698
                     /gene="mdcA"
                     /locus_tag="IV247_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAP8777909.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            13800..14099
                     /locus_tag="IV247_001842"
     CDS             13800..14099
                     /locus_tag="IV247_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAP8777910.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            14089..15765
                     /gene="mdcD"
                     /locus_tag="IV247_001843"
     CDS             14089..15765
                     /gene="mdcD"
                     /locus_tag="IV247_001843"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAP8777911.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            15769..16362
                     /locus_tag="IV247_001844"
     CDS             15769..16362
                     /locus_tag="IV247_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAP8777912.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            16386..17462
                     /locus_tag="IV247_001845"
     CDS             16386..17462
                     /locus_tag="IV247_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAP8777913.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            17487..18356
                     /gene="mdcB"
                     /locus_tag="IV247_001846"
     CDS             17487..18356
                     /gene="mdcB"
                     /locus_tag="IV247_001846"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAP8777914.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            18467..19231
                     /locus_tag="IV247_001847"
     CDS             18467..19231
                     /locus_tag="IV247_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAP8777915.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            19720..21813
                     /locus_tag="IV247_001848"
     CDS             19720..21813
                     /locus_tag="IV247_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP8777916.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            21838..22140
                     /locus_tag="IV247_001849"
     CDS             21838..22140
                     /locus_tag="IV247_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8777917.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
BASE COUNT         7177 a         3787 c         4723 g         6478 t
ORIGIN      
        1 gttcgctttt tttaggttat ctattttaga agactgattt attgaaaaat tgttcgcttt
       61 tttgcgttga attatcagaa aaatccgact ttatttattt ttgtttgtaa aaatctacgt
      121 ggatttgatg ttataaaaaa aaaattctca tttttggcgc tatagtgaag aggtgttaca
      181 gataaacaca tgtatctgca agacattgac caacaaaata tggagggaag aaaaatgaaa
      241 gatatggata tcaaggccgt ctttataggc gacaaagctg aaaatggacc agtttataaa
      301 atgttgttga acaagatggt agatgaacat cttggatgga gagaaaatta tattccttca
      361 gatatgcctg caattagcga aggagataag ctcactccag attatctagc cacccgcgac
      421 catatgatag aggtcttgga tgaagtcagt cagcgattac gcgcaggatc gatcccatgg
      481 cattcagcag gccgttactg gggacaaatg aatgctgaaa cattgatgcc tgctttatta
      541 gcttacaact atgcgatgct gtggaaccca aataatgttg cattggaatc ttctatggca
      601 acttcgcaaa tggaagcaga agtaggacag gattttgctt cattattcaa tatgacagac
      661 ggatggggac acattgcagc agatggttct attgctaatc ttgaaggatt atggtacgcc
      721 cgttgcatca aatcgattcc tttagctgtc aaggaagtgc tgcctgaaaa agtgaaaaaa
      781 atgtctgagt gggaactttt gaatttatct gttgaagaaa ttttggaaat gaccgaaagc
      841 tttacggacg aagaaatgga tgaggtcaaa gcagcttctt cccgtagcgg aaaaaatatc
      901 caaagattag ggaaatggct ggtccctcaa acaaaacact attcttggat gaaggcgtta
      961 gatatttgcg gagtcggctt agatcagatg gttgctatcc cagttcaaga ggattatcga
     1021 atggatatta atgctttgga aaaaacaatt cgtgaattag ctggacaaaa aatcccaatt
     1081 ctaggtgtgg ttgctgtcgt aggaacgaca gaagaaggtc aagtagacag tgtagataaa
     1141 atcgtccaat taagagaaag gttaaaagat gaagggatct atttctattt acatgttgat
     1201 gccgcatatg gcggctatgc gcgttcactg tttctaaacg aagcaggaga atttgtgccg
     1261 tatgcttcct tagctgaatt ctttgaagaa catcatgttt tccaccactg cgtaacgatt
     1321 gacaaagagg tatatgaagg gttcagagct atttcagaag cggattctgt cacgatagat
     1381 cctcataaga tgggctatgt cccttatgct gctggaggaa tcgtaatcaa acataaaaat
     1441 atgagaaata tcatttctta tttcgcgcca tatgtgtttg aaaaatcagt aaaggctcca
     1501 gatatgttag gagcttatat tttggaagga tcaaaagctg gagcaaccgc cgccgcagtc
     1561 tggacagcac atcgtgtatt gccattgaat gtgaccggat atggtcaatt gattggggct
     1621 tcaatcgagg cagctcaaag attcagagaa tttctcgatc atctgacttt tactgttaaa
     1681 ggaaaaacaa tcgaagtcta tccattgaat catcctgatt ttaatatggt caattgggta
     1741 ttcaaagaac aaggctgtac agatttgaac gctatcaatg aattaaatga aaaaatgttc
     1801 gatcggtctt cttatatgga tggcgatgtt tatggtgaac ggtttattac ttctcatact
     1861 acatttacac aagaagatta cggtgattct ccgattcgtt ttgttgaaag aatgggtcta
     1921 acgaaagaag aatggaaaaa agaacagaaa atcactctgc tacgtgcggc tatcatgact
     1981 ccttatttga atgatgatcg aattttcaat ttttatacaa aaaaaatcgc caaagcaatg
     2041 gaaaagaaac taaacgaaat catccaatag aactgatttt tgtgtgaaag aataacaaga
     2101 gacatatgga gaacagacaa ttatttgctt catcaaaaca attgtatttc cccctgtttt
     2161 tcatatattc ctcgccaaga tattcttttt tataaaacgt tctataagtt ggatcgttcg
     2221 tttcatgtat caaacccagt tcactaagat cgttcaatgg atgtttcggt gatactgggt
     2281 tttgtactga atcgatgaaa ccttgtcatt tctgttcttg aacaaaaata aaagaaaaca
     2341 aaaacatatg tcgctattga aagaaggaat aaagatgaaa gatactggtt catcaaagca
     2401 aatcacgacc atgaccttta tcggaatgac ctgcgccctt gtagcaagtg tacgaaatat
     2461 tccagatgta gcagcaacga gctggacgat gattttttac atgctggttg cagtattatt
     2521 gtacgctttt ccaatctcat tgatctctgg tgaatttgct ggaatgtttc cccaaaaagg
     2581 tggtccagaa ctttgggtat cgaatgctct aggaaaaaaa tgggggttcg ttgtctcatg
     2641 gctgttatgg gtccaaatgt ttcctgggat ggtgatggtc gcttcagcac tcgctccgtt
     2701 gtttggtaat attatcgata atgtgccgct tggtttgaac agcaaattta cattagttgt
     2761 cattttagtc gtttattgga tcatcacttt tttgaatttg aaatttgata tggcaaaaat
     2821 cggcggaaaa gtcggtgttt ggttaggatt gtacgtgcct ttgacgatga tgctcttatt
     2881 agggtttgct gcgtggatca aagttgggat cgtaccaacc ggaacattag gcagtttttc
     2941 atgggacaaa ttaataccag atactacgac tgcatcctca tttgtttatt ttgcgccgat
     3001 catgtttatt tttactggta tcgaaatgtc ctctgtgtat atcactcgat tggaaaatcc
     3061 agttaaaacg tatattcgcg gagtatttgc cgcgttaatt tttatatttt tcgtcaatat
     3121 tttgaatgct ttagttgtag caaatgtcgt accaaaagga caaatggaat tgaataatat
     3181 cgcgcaatct atttcgattt attgccaaat tcttggtttg ccacatctta ttgtgaatct
     3241 gtttagttta cttgtcttca ttggtgtagc agtccaatta tctgcttggg cctcaggacc
     3301 agcaaagaca gtgacagaaa gtgctagaaa aggtgcgtat ccaccaaaat tcaatttttg
     3361 gaagacgaat caatttgacg tatccaaatc cgttattttg actcagtctg tcattatctc
     3421 aatctttgca cttttttatt tgctgattcc tggcgtcaat caagcatttt tgatgctcgt
     3481 aaattcaact actgttatct actgtatcgt ctatgtcatc atggggatcg cagttttgag
     3541 attgcgttat acacatgcaa agttgaatcg accatttaga attggaaaaa aagagaaaaa
     3601 gagcaatctt ggagtttgga ctgtcgttat tgttttattt gcggcaatcg gattttctgt
     3661 cggactgacg atgaaagctg gaacatggat caatctaatt gcagtcacag ccatcagtgt
     3721 cattttgttt gtagtaccac tctgtattga aaaaataaag aaaccttctt gggaacagga
     3781 agtcgccatg ctgcttgaaa aagaaaaagg tgaacttgat ggaaaaaccg gcaatgaaac
     3841 ggactctaac taatcgaaat atccagttaa tcgcattagg tggcgcaatt ggaacaggat
     3901 tatttttagg ttcagcaaag gcaattgcat cagcagggcc agctgttatt ctcgtttatt
     3961 tgatatgcgg tttggctatt tatggtttga tgcgggccat gggtgattta tttttagcaa
     4021 atagtacatt taactctatc agtgatttcg ttgactatta tttaggcaaa aagtgggcat
     4081 tttttgtcaa ttggacttat tggctttgct ggcttggagc tggacttgcg gaattaacag
     4141 caataggatt gtatatccat ttctggtttg tacatgtacc agctttgata agcggaggta
     4201 gcgcactaat gatcttaatg gcagtcaacc ttgttgcagt caagtggttt ggtgaattgg
     4261 aatctttttt ctcaatcatc aagatcttgg ggattttcct ttttatctta ttgggtatag
     4321 gattgagtgt ctcagatttt catacccagt ctatcggaat caaagaaaat tttggacaga
     4381 tcgatgaatt actaccgttt ggctttgctg gaatggtagt ctcatttcct atggttttat
     4441 tcagttttgc tggaattgaa atgatcggct taaccgttgg cgagacaagt gatccgcaaa
     4501 agaatttgaa aaaagcaatc aactgtctgc cttggcgtat tctctttttc tatataggaa
     4561 cgattttgtc gattttattt gttgttccat ggcattgttt agatttaaaa gaaagtcctt
     4621 tagtgactat gctgcaagtg atccattgcc aaggcggagc agcaattgtc aatctaatga
     4681 ttttggttgc ttctttgtcc tcagcaaata gtgcagtcta cagtactagc aggattttat
     4741 atcaaacagc aaaagaaaaa agtttgcccc attggttttg tgcgctttct aagaaaggcg
     4801 tacctttaca cggtatttta attacaggaa tgttattttt tgccggtctt tgtctacatt
     4861 tttttgtagc tgatagtcga ttgttttttc agcttttggc aggcatgaca acgagttgtt
     4921 ttctatttgt gtggtcttcg atcttgtgtg cacatgttca atttattaaa gaaaagaaac
     4981 ggaacactac acaaagatat aaagatcttg ctttgttggt gttttttact tcaattgctt
     5041 tcagcttgtt attcgaaagc acgacacgct ttattccttt tttattggtt atctggtttg
     5101 tattcttaaa ttttgtatat aaaagaataa gcaatagaaa tatcagttag gcccagatac
     5161 attttgcaaa acgaattatt tcagaccccc ctgaaataat cgatacgcct gaaaaaactt
     5221 ccctgcgtat aataacagta tcaattaaac aggaggggat aggatgaact ggtacagatt
     5281 gacggtagaa gatgtattga aaaactatca aacatcaaca gaaggcttgt cgcaagaaga
     5341 acgacagaaa cgcctgactg atcaaggata taatgaaata aaagcaaggc agcagacaaa
     5401 acgatggaaa aaaatagcaa agcattttac tgatctgctg atgatcgtgt tgatcattgc
     5461 ctcttttttg aaatttgctt ctagtgaata tattgaagga agtattattt tattcgtagt
     5521 gatcgtcaat ggactcgtta gttactggca agaaagaaaa gcggaagaat cattgaatgg
     5581 tttgaagcag ctgatgggac aagaagcgat agtactatca aacgggatgc aagaaaaaat
     5641 ccctgcgaaa gaactggtaa aaggagatgt cattgtctta cgtgccggag atgtcgttcc
     5701 agcagatatc cgattaatcg aagcacatga tttattagtg gaagaatcga ttttgacggg
     5761 agaatcagaa gcatctgaaa aaagtcatgc cgttttgaca gaagaagaat cgatcggcga
     5821 ccagaaaaat atgggttttt caggaacgct tgtacaatct ggttctgcag tcggtgtcgt
     5881 tgtagaaaca ggaaacacga cagaaatcgg taaaatcaat caagcattgc aatcggtcga
     5941 acaacaaacg acgcctctga ttaaaaaaat caaccgatta aacaaacaaa tttttcaagg
     6001 cattctttgt ttgagcctat tcttagttat ttttacgaca tttcgatatg gtatggattg
     6061 gcatatcctt ctttcggcta cgattgcttt ggtggtgtca atggtacccg aaggattgcc
     6121 agctgttttg accatgattc tatccgttgg agtacatgaa atggcaaaag aaaaggcgat
     6181 catcaaagga cttccttcgg tagaaacatt aggttctatg acagtgatct gttcagataa
     6241 gacaggaaca ttgacaaaaa atgaaatgac agttgtggat gtagctgcaa aagaagaagc
     6301 ctgcgtgttg tccattatga aaaactgtca ggaattaaaa acaaaagaag aacagaaaac
     6361 agaaaattta agtggcaatc cgacagaagt tgcgctgatt cgacacacag aaacagccaa
     6421 tctacctttg aaaaaaatag aagcaaagat tccgttcagt tctgaataca aatatatggc
     6481 aacgatgcat gcggaggaaa aaggagcggt gatttacgtc aaaggtgcac cagaagtatt
     6541 atttgctaaa tcaactcttt cttctgcaga acaagaagca tggagtcaga cagctgcaga
     6601 atttgcgaga aaaggtcagc gtgttttagg atttgcttat aaaaaagtag attcgaaaca
     6661 ggaactcacg catgagactt taacccaatt aacttttgca gggatcgccg gattgatcga
     6721 tccgccaaaa gaaagtgcag tcaaagctgt aaaggaatgc caacaagcag ggatctcagt
     6781 gaaaatgatt acaggggatc acaaagacac agcaaaagcg attgcggaac aaattggctt
     6841 aaaacatact gccaaagttt tagaagggat cgatttggat cttatgtctg atgaagagtt
     6901 gattcagcaa gtgccaatag tcgatgtctt tgctcgaacg acaccagaac ataaactgcg
     6961 aattgttaaa gctttgcaga aaaatggaga aatcgtcgga atgaccggag acggcgtaaa
     7021 tgatgcacct gctttgaaaa gatccgatgt aggaattgct atgggaatca aaggtagtga
     7081 agtaagcaaa caggcagctg atatggtttt aggagatgat aatttccata cgattgcaaa
     7141 agccgtaaaa gagggccggc ggattttgga taatttgcaa aaaaccatca atttcttctt
     7201 gccaactgca ttagctcaag gattgatctt gatttgggca cttatgctga atcgcccgct
     7261 acctttgtcc ccagtacaga ttttatgggt caatatggta acgacaatca ctttatctta
     7321 tgcactaggg ttcgagtcgc cagatccaga aatcatgaag cgcccaccaa gagatccaaa
     7381 gcaaggaatc ctatctggtt atcatttgtt tcggatcatc tatgtctccc tactgatcat
     7441 ggttcctgcc tatttattgg ctatgcagtt tgaagggcaa agcttacagc aaaccattct
     7501 tctgcaaaat atcgtcttag ctcaagctgt ttacatgatc aattgcagag agttgtccaa
     7561 gccgtcattg aaccaaggtc ttttgcaaaa caaagcgctt tttctttcat taggtatcct
     7621 tgctttattg caagggatgg ttatttttct accagtcggc cagcagctga tcggtacagt
     7681 aagtttaacg atgacacaac agttattgat tggagcaaat gtgattcttc tattcttagt
     7741 tgtagaaata gaaaaaggga tcatgaacaa actgtttcgc aaaaaagaaa aaacagctag
     7801 agaatacagt cgctagccgt tactataaaa aagttttgaa agtgagttca gtcaaacgat
     7861 tgagctcatt tttcgttgga atagaagaaa tgtatacaat agggatatct agagaatcta
     7921 gagggaaatt ggaaatcaag atatctgttt tttcaaaaga aatagtggaa agatcggtta
     7981 gttcgatagg taagaagcag acacgttttc ctaagtaatc ttttaattcc tgctgcaaga
     8041 acgccttcca tgaaggttct ccttggaaaa taaaatagac attgaacaaa ttcggtttga
     8101 tagaaaaaat cgtttgttgg atcagtagag acaagagttg gagaaagtaa tttggttcat
     8161 tgatttctat ttcttttttt agatcgttca gtaaattctc tttctgttga tacaagtgtg
     8221 catattgctg atcatatgtt tctaaaaaac gatggtattg acggtccaat gcttcaatca
     8281 atatcggcga ttcatgatat tttaatatga ggagagccaa gttatcagtc agagatgttt
     8341 tttttagttg atcaagagac agctctgaag ctagttcctc tacaaacgtt tcgaggacct
     8401 ctaaaaatgg agaatgatgc aggcagcgag cggtttcttg tccatagtta ttgttgtaat
     8461 tccaactttc cagaaagcag aaagaagcaa aagaaagctc agtgtcttta agataaatac
     8521 cttccgttaa atacaatttt ttcaatgcag agtcatacag ggaggagagt gtgctgttgg
     8581 tatggataga agaatctaat cggcaattcg cttttatacg gatcgtattg ataaagaacc
     8641 aacatgttcc gaaaatctct tctgtttcaa ccgctagttc agtttgactg aggtttctta
     8701 aaaattgaaa ataatgggat tcatggatcg aataatcctc gaaaaaatag ttgttatgag
     8761 tgaaaggaat cagcaatcga tgataaaaag tacgaatcgc acgctctttt ccaagaatac
     8821 aagctgtata tttcgtcaaa tcgatctgca agtcgtaact tgctaaacgc tgattgattt
     8881 tagtcacatg tcttttcaat gtcccataag aaagcccatg gtcggctaga aaatcaggga
     8941 cagcaacttt ttttgtaaaa agcagatttt tcagcagttc gacactgact gattgtttga
     9001 aaaaaatctc ccaaaggtca tttgttaata aattttgctg gttttgcaaa ctgattcctg
     9061 cttgatcgct gatgatgtcc cagccttcgg gcagctgaga gcgaatagat tgaaggtcag
     9121 aaaaaatcgt tcgttcggtt gtccctatga tttcagctaa ttcatttacg gttatctttg
     9181 tatgattcag caattgttct actaaagaaa catgacgtcg aatatctttt tcggtaatga
     9241 tttttttcat tagtgaatac atcaatcaga ctcccttcta ttctaagaat acggagaaaa
     9301 taaacggtgg tcaagtttca actgattttt caatcattgt ttatttttct ggagcattta
     9361 ctgatgataa gaatttcatc atgaacttga aaaatgatac cactaaaatc aaaaaatagg
     9421 tgggcaaaca ataataaaca ttttaatagt agatggtagc aagaccaaca caggcggatt
     9481 gaacatttaa gagttgataa aaaagaaaaa tatcgaagta tttgattagc aactacttgt
     9541 attcattttt ctattcttac gtggtatctt tgaataaagc gaatcaataa gaaagagatg
     9601 gaagcagatg ctaacgaaaa aagaactaat cgatcacatc aatgaaacat acaatttgtc
     9661 tccagaatat atttttaaaa aatttcctga ttattgcgta ttcagacatc aaaaaagcaa
     9721 aaaatggttt ggggtcctga tgtctgttcc agaagaaaaa atctatgatg atgcaagtga
     9781 ccataggatc atcaatattc ttgatttgaa ggtcgatcct gaacttggcg gaattttgcg
     9841 aggaaaagag gggtattttc cagcctatca catgaataaa gaacattgga tctctattga
     9901 tctacgtaaa atagaaaagc tagatgaact agcagatttg attgatggaa gttttaaatc
     9961 aacgcaatga atgtctagga caatagagaa gaggttgtga cagaaatgtt tagcttcaag
    10021 aaataagaag gaatttacga aaattgcttc tcaaattttt gggaaatttc ggcttatttt
    10081 ctaagaaatt acttctggaa caccgtttgt tcgaaaaaag agctgcgaca agaattttgt
    10141 cccagctctt ttttttttat tttcctaaat aaaattcccg catcgttttt gcaatcattt
    10201 tgatcaaagg atgatcttct gtgaagctag aagataaata aattgttcgt gtcaagggca
    10261 aatctttgat ttcccgcaag acgaaaggat catttttatc gtaagaccaa gaaacttttg
    10321 ggaaaaagct aacagctaat ccttgcttga caatagaacg taatgtcgca ggatcatctg
    10381 tcgtcgaact gatcgtcaat tcgatacttt tcgcttcaag tgctgcatcc agtgtatgac
    10441 ggaaggcgtt gctctttgac agcatcaaga tgggggtgcg ttggatatct tctaattgca
    10501 gatgttcttt tttagcaaga ggatgtttct taggaatggc gacataaaca ggttctttca
    10561 tgataggaat attggtcatg ccaggcttca tctccgaagt gatgtacagt acaggatcgg
    10621 taatatcatc tggtaaatga tgttgcatga tcgttaattg gatatcagga tgcttttcca
    10681 taaaaattcg gaccaagttc ggaatagaaa cagaggctac ctctaaatac aatatgacgg
    10741 aagtaacgat cttctcattg accgaagaca ataatttgat tccttcttct aatgtgtcta
    10801 atgtcttgac tgcatatgga taaaattctt ctgcataaac ggtcgtctca atgtttctgc
    10861 cgctatggta aaacaaaggg acacctaatt cgttttccaa atgtttgatc gcacggctca
    10921 aggcaggttg agaaataaat aattctttgg ctgcttgggt aacatttttt gtttcgaata
    10981 cttttttaaa atactgcaga tgaagcaatt tcataattat ccatcccatc catacctttt
    11041 tgttatggtt ttttcttttt ttggcatttt ttattatagc agaaattgac taagatagca
    11101 gtggaaaaag taagtgctga ggaggcgagg ctcatgagta caggtttctt atttcctgga
    11161 caaggcagtc agaagttagg gatgttagcg gatatccctg aaatgtacat ggaacgttta
    11221 gaaaatgtga cggggtatcg gttagttgat aaagaagaaa actatcaaga tacggttttt
    11281 atccagctag cattactgac aaaagcagta ttttatttag atgaaatgga aaaaaacggt
    11341 attcagcctg atattgcagc aggacattcg attggtgctt tttgtgcagc tgttgcttgt
    11401 cgatctttga gatttgagaa tgcagcggaa ctagtctatc accgcgcagt cttaatgaaa
    11461 gaagcttatc caagcggtta tgcaatgggc gtagtagtag gtgtgacgag aagcgaagct
    11521 gaggaaattg tagcaaaaac atttgatcct gaccatccag tttatctgtc aaatgaaaat
    11581 tgtccgatgc aacatacgtt atctgggagt atcgaaggtt tatccgttac gttggcagaa
    11641 gccaaacggc accaagcgca aacagcgaag ctgctgaaag tccctgtgcc atctcactgt
    11701 ctcttgatga atgatacggt caagaaattc aagccgtata tgcagtctgt ccatctatct
    11761 gatgctagat gtttgtatat aaaaaatacg gatggaagag ccactcagga cgcagaagag
    11821 attcgtaaag atttgttgga aaacctagct ttccctgtgc agtggaacca aatgatggac
    11881 ttggcaaaag aattaggaat ggatctgacg attgaatttc cccccggcaa tactttgaca
    11941 aagctgattc atacaaaatt tggtgtagat aaagggatcc gtacgatcaa tctagaccaa
    12001 catggtattg atgacgccac gtttctatat caaaaatgga gatgactaat tatggaaaaa
    12061 ccaaaccgca actgggcttc taaacgcgaa aaaaaagcgc agcgattgga aaaaatcagc
    12121 aacttgctca atggcaagta tgtagaaact gaaaaaatcg tagaagtaat ggaaaaactg
    12181 attgctcccg gcgacaaagt tgtattagaa ggcgacaatc aaaaacaagc aagttttctt
    12241 tcagaaagtc ttgaaaaagt agatgcagaa aaagtgaatg atctgcattt gatcatgtct
    12301 agtatctcac gaccagaaca tttgaatatt tttgaaaaag gaatcgcaag caagatcgat
    12361 ttctcttatg ctggtgcaca aagcttgcgt gtcgcccaaa tgattgaaga cggcacaatg
    12421 aaattaggtg atatccatac ttatttggaa ctttatggac gcttattcat cgatttgatt
    12481 ccaaatgttg ttttagtagc tgcagataaa gcagataaag acggcaactt gtacacaggt
    12541 ttcaatacgg aagaaacacc aacaatcatc gaagctgcag ctttcaaaga tggtatcgtg
    12601 atcgttcaag ttaacgaagt tgtcgattct ttaccacgtg tagatatccc tggtgactgg
    12661 gtagacgttg tcgtgaaagc agataaacct tatcaattag aagctttatt cacacgagat
    12721 cctcaaaaca tcacagaatt gcaaattcta atggcaatga tggcaatcaa aggaatctac
    12781 gcagaacacg gcgtacaatc tttgaatcac ggtattggat tcaatacagc agctatcgaa
    12841 ttgttactgc caacttatgg tgaatcacta gggttaaaag ggaaaatcgc gaagaactgg
    12901 gtattgaacc cacatccaac gatgattcca gccatcgaat caggttgggt tgaaagcatc
    12961 catagttttg gtggcgaagt tggtatggaa aaatatattg ctgctcgccc agatgtcttt
    13021 tttgtcggta aagacggcac gatgcgcagt aaccgaacat tatcacaagc tgcaggacaa
    13081 tatgccatcg atatgtttat cggttcaacc ttgcaattag attacgaagg aaactcttct
    13141 accgtaacaa aaggacgttt atctggtttt ggcggtgcgc ctaacatggg acataatcct
    13201 ggcggtcgtc gtcattctac accagcatgg caatcacttc gcgacaatga tgatccatta
    13261 gggaaaggcc aaaaattagt tgttcaaatg gttgaaacat atggttcaaa taaaaaacca
    13321 gtctttgtcg aagaattaga tgcactagct gttcaaaaac aagcaggctt agccaatgca
    13381 ccagtgatga tttatagcga agatactaca catatcgtga cagaagaagg aattgcttat
    13441 ttgtacaaag caaaaacaaa agaagaacga caagcagcaa ttgcagcaat tggcggtgtt
    13501 acacctcttg gaatgactag cacgaaagaa tcactagata gcttgcgtgc agccggcatc
    13561 gtgaaattac cagaagatct aggcatcaaa cgtagtgaag cgaaacgttc attattagct
    13621 gcacaaacca tcgatgattt gatcgaatgg tcagatggcc tgtatgaacc accaatgaaa
    13681 ttcagaactt ggtcataaaa ttggcgagaa aaaccttttg tcgccattta cccagatgga
    13741 agaagaaaga cgaataagca gaacgtgtat gctctgtttt acaaaaaggg gagagatcca
    13801 tggaacaatt agcttataaa tatccaacga aacagccgat caataagaaa atccatgtag
    13861 gagtggtcgg ttcaggagat ttggaaatct tgatgtttcc aacagaaaaa acagaatcta
    13921 ctgtaaaaat ttgtacagga agcgatggct tcggcgaagt atggaacaat gtattaacga
    13981 gattttttga tcgttatcca atttcagcag atatcgtgat caatgacttc ggtgctacac
    14041 caggagttgt gtacctacga ttgacgcaag caatggagga actttcagat gaaaaatagt
    14101 tttgtagaat taaacgccag agaacgagtg aacgctctac ttgacaatgg acgtgaattg
    14161 attgatccat tcgatcaaat catcgcgcca agtttaccag cacaaggaat cgtccctgaa
    14221 agtgatgacg ggatcgttgt cagccgtggc acgatcaatg gcaaacaagc agtagtaatc
    14281 gctatggaag gaaaattcca aggtggcggt atcggtgaag tttccggtgc aaaaatcgtt
    14341 gctgcacttg aacatgtgtt agaagaaaat aaaaaaggaa atgagatctt tccagtcatt
    14401 gtactagata caggtggtgt tcgtttgcaa gaagcaaact acggactgtt atctatttca
    14461 gaaatcggaa atgtaatcat tgctctaaaa caatatgtac cagtcatcgg tttgattcca
    14521 ggacgtgtag gatcatttgg cggtatgtcg attacttcag cattaatgag ttatttaatc
    14581 gcaacaaaaa aagcgagagt cggtttgaat ggaccagaag ttattgaaca agaagcaggc
    14641 gttcgagaat ttgattctag tgacaaagac ttgatttgga atacattagg atctcgtcaa
    14701 cgggtagaat caaaaatcat tgatgaatta gtgactgata gtgtagaagc aatcaaagct
    14761 gctgtcatcg cagcaatgga cgaacgaaaa gacacgcatc gcagtgaaag ctcagcattt
    14821 tatttatcat tacttgattc tttagatctt tcaaaaccaa tggccattga cgattacaat
    14881 gaagcaattg ctaaccatca agcaaaatca attgacttac cagaagtagc agaaggaaat
    14941 gaaccagctg cagcaagtgt tggttacgat tggtttgcgg cactaactgg catgaaaaat
    15001 ccaacttctt ctattagtac tgtgtattat ggtaaatcaa gtaaattcat agaagaagcc
    15061 atcgtgacat caatcgtacc tgatcctaaa aacccgatgt accgtgtacg aaacggtgaa
    15121 gttggtttag ttgaaggttt ccgcatggga caaattttgg atcacgttta cgaagaagat
    15181 aaagacaaaa aagtgaaacg gccaatcatc gttgtgatcg atgtaccaag ccaagcatac
    15241 ggctataacg aagaattgat cggtatccat gtggctttgg ctaatagtgc tgctgcttat
    15301 gcaaaactcc gtcaagctgg acatccagtt attggattga ttgcaggaaa tgctatttca
    15361 ggtgcatttt tagcacatgg attacaatca agccgcttga ttgctttaaa caataaagaa
    15421 attaccgtcc aagcgatgtc taaagaaagt gctgcacgtg tgacaaaacg ttcggttgct
    15481 gaaatcgaag cagctgctga aaaagttcca gctatcgctt acgatatcaa taattataca
    15541 aaactaggag cgatctatca tttcctagaa gatatcaccg accaagctgc ttcagaacaa
    15601 aatgtcagca aagtcatcga agcaatcaat gaagccattc aagatgtccg caatacaaat
    15661 gacacgatgc tgacaaatcg atacacaaac gaaacagcca aaacattcgg acgtgtcgaa
    15721 acaaataaag tccgtgccaa aatggacgaa gaatgggatg cataaaaaat gattcaagca
    15781 catgatattg tcctttttga taaacatgaa atagaagggt tcaccttgcc tgaatgggtc
    15841 gttctaactg aaaagatgtt cggaacagtt cgtcgtgcgt cgacggatca cgcagaaata
    15901 ctaccgattg gtttacgtgg acctgcccgc aatcagagat ggtcaacaga aatctcaaaa
    15961 catgccgtca aacaagtcat tcatccgtgg gacatcatcg aacaagaatc gtatagacaa
    16021 gagaaaatca tggattatcc tgtttatcag caatttcatg ctgctagaaa catattatca
    16081 ggatgtaaat ggggaatcgg cggcagctta ggatttgaat tatcgactgg gattccggca
    16141 gtaaaagaaa ccagtgattt tgatttgctg ctgtacgcaa attcaccgat tgaattgcca
    16201 attcaggtaa ttcaatcgca tcctgctttt tttgagcaat tcgataccca agtagtgact
    16261 cggcaaggcg gtttttcact aaaagagtat ttgagagagc cagagaaaaa aattctattg
    16321 aaaacagtcg aaggacctaa attaaccaaa gaactttggt aataaagaaa aagagagaga
    16381 aagagatgtt tgaaaacaaa aaaatcaaaa tcaacgtaag tgacttccag aaagtatcag
    16441 ctgtaacggc tgttatgatg caaacgatat tgagctttgc tttggcaaac agccacagcg
    16501 caacaaatac agcttttctt ggtacctttt acgtcatcgt taaatacaca gcaccaatgt
    16561 ttatttttgc aattgtttat aatatggtaa aaacaagtca gcatttgtct tattgggaat
    16621 ttttgaaaga aaagtttttt gaactagtcg ttccgtatct tctatggacg acggcttatc
    16681 tttggctatt tccagcagtc caacaacaaa ctccgtatac ggatactgca agctttttac
    16741 tgaaatatgt cacaggtgat ggagcgcctc atttatggta taccgttatg atgctgcaga
    16801 tccaattgtt catgccgttt tttgtttggc tcggttacaa agtatttgct aataaaaaat
    16861 atgtatggcc agtattgatt ggttcaacga ttttatatgt cgcatggtat ctgttctatc
    16921 aaacacaagt attgactggg ccttatcatg acagctggta cttattagat cggtttgttg
    16981 ctagtttcat gatttatggg gtagccgcat ttgtttatca cgaaaaagtc tatcaatatt
    17041 tggacagagt acgttatctt ttcttacctg ttgccttagt tatcgcgttc ttttctgtcc
    17101 gttcattgct cgcacatcca ggagacttga gctttgcgaa tgcaccttac ttgaatacga
    17161 tccaaagttt atacagttta gtcatcatct ttgctgtctt cataggcgct tccaaaatga
    17221 ttgtaaacga ttcgccaaaa ttgccattgt tcaaatggtt gtctgtttat gcttatcgta
    17281 cgtatttagc caatgtattt gttttccaag tactgttatt attgtttaaa gatagttggc
    17341 tgcagttacc ttcaggagtc atgattcttg tagcttatct aatgacggca agttgtgcat
    17401 ttgctttaag ctggttactt catatcattt gggtagctat caaaaaaggt ttcagcaagt
    17461 aatagtagga agaaggagtg agtgtcatga taaatgacga acgaaaaaaa gcagtgactc
    17521 agcaactcgc tgcgttggta gaggaagcat tgatcgcaga agtaacatta agtccaaaac
    17581 ctggattagt ggatgcactg gatgcgggtg cacattccga tatggactat gctttatttc
    17641 ttaagtctgc taagacgcta actccttttt ttgaagaaat ggctcaaata gcatggcatc
    17701 atgctattga tcaggagtta agagaaagaa ttgctgagat tgggcgaaaa gcggaaaagg
    17761 caatgctgat tgctacaaat ggcatcaata cccataaagg agcgatttgg gcaatggggc
    17821 tattgatcag tgtcattgca caaaaaatca gtatggaaca gcctttttct ttatcgaaaa
    17881 tacttgatga tgtcagcaag cttgctgctt ttcctgatac aaaatatcgt cataaaaaaa
    17941 ccactcatgg tcatagggtg aagcaaaaat atggcgtaaa tggtgcatat gaagaagcga
    18001 tattagggta tccgcatatc aggcttgcaa ttcaagcagc taaggagcta gataatgatt
    18061 catatgtaca gaaacaaatg catatgttgt tagttttgat gagttcgcta gatgacacat
    18121 gcgtattgca tcgcagtgat cagaaaacgt tagtggaaat gcaaaaactt gctcatgctg
    18181 ctagtcaaag tgctttacca aatcgtccat ttacagcact gttgcacttt tgtcaggaaa
    18241 accaaatctc tcctggagga agcgcagatc tcttgtctgc aagcttgttc atgctgaaga
    18301 tagaggaatt atggactgcc gacacataca tttgtccgat gttactcaat gactaaataa
    18361 gacaaaattg ctaaaaaaac agtttatgca tctggttatt gactcttcct tttagcaagg
    18421 ctatcataaa aatagttttt tgtaataaaa gataacgagg aagacaatga aaaaaatcaa
    18481 ttatttagca atcattttgt tagtgggcct atgtttgttt gctctgccga cccaaggatt
    18541 cgctgtcgcg ggtggtcatg gtggaggtac atctagcggt cgaagttcgg gcagaggttc
    18601 atttggtggt cgttcatatg ggggcagtta ttttggcagt cgttcttacg gcagagattc
    18661 ttatgggtat tatggtccag gtacaggtcc ttctagttat gagcaaacgg gatttcttgc
    18721 attagctgtt ttgtcagtag gaggggtttc tgctttttct atcgtcaaga ataaaagaca
    18781 aaagaaaagg ctgaaagcgg agatattgtc ccgcatgtca ggaacggcta aagaaaagaa
    18841 agcacagttt ctggaaatcc aaaagatatt catgaagatc caatccgctt gggaagaaga
    18901 aactcctgaa aaagccagaa gctgttatac agacaagctg tttgccgaac atcagaaagt
    18961 tctgcaaaaa aacaaagcag acggtgtaag aaatcatact aaaaaaataa ctattcagga
    19021 agtaggaaat tatcgacaga tccacgaaaa tagttttgcc atccgaattg atttcagatg
    19081 ctgtgattat tcagtcgatc gaaaagatgg gcgattgatc tcaggaagta agacacaacg
    19141 acagtatttt tctcagatat ggtattttga tttttctgag aaagaaaaaa gatggcaagc
    19201 cgattttatt cagccgatta tgctggattg acaggaaata tttatgaacg gagttgtgac
    19261 aaaagccttg tcgcagctcc tttttctgaa taaacggtgt gccagagata gcctatcggc
    19321 aataaggcac gattttgtaa aatatgggaa gcgatttttc aaaatcgctt cttatcgctc
    19381 aaggctgaac atctctgtca caacctctcc actaaatggt gtttaaaagc ttgcatctgc
    19441 gataataaga acaaccaaac gaaaaatagg aagcactatt ttagtttagt tgttcttatt
    19501 acttgatgca ggacagcttt ttcacaacct cttttttgta tagatgggca aaatttttgt
    19561 aataataggc gtatgtcttt ttcgttgagg agatgaaaaa gcatatggct tattttttgt
    19621 ttgctttgct tactttcttt ccacttcaag tggcagaaat atctactttt tattatgtaa
    19681 gcggaaccat atacttgaaa tcagaaggag atcatttcta tgaaaacaag aacaattgaa
    19741 atacttcaca gaatcatgac agcccaatat ccagtaaaag caaaacatct agcaagtgag
    19801 ttcaatgtca gtcagcggac gatccgtcag gaagttcttg aagcgaatat ctggttgagc
    19861 agtaaaaagc ttccggagat ccgtacaatt cgcaacaaag gttttttgtt gaagctggaa
    19921 aaggaaaagc aagctcgatt agaaactgaa ataaaagatg tgaaagacgg ctttttgaat
    19981 cggcgcgaac gtatttttga tctagtttta gccgttgctt acagccgaat cccgatccat
    20041 ctaaacaaaa aagaagaaga atttcaaatc tctaaaagta cattagatga agatatgaga
    20101 cggttacgag caacactggt cagttatggg atagagatcg tcagttatgg aaagcaagga
    20161 ctggtttata aaggttcaga acgatctatc cgtacgatga tctatgattt tatcaatcag
    20221 aatctgggaa tgaccgactt ctttatgaat caagatgcac aattaacacc ggctcaaaaa
    20281 atctttctcc attattttcc tttggtggaa atcaaaaaaa tacaggagat ctattccaat
    20341 aagttgttga aacaagaaga tgatatttat aaaaaacaaa tcctgctttt tacattgatc
    20401 tggatccagc gaatcaggaa acatgaattt atctctgcca ttaattggaa aaatacagaa
    20461 ataaaagaca gtagcttttc tgcatttatt gaccacatga tcgaggaatt tcaattggac
    20521 aatgtcccgc aagtagaaag aaattatctt cgtttcacta ttgagacgtt caatgctaga
    20581 gatatctcca attcaatcga gtgggtacaa gctcaattac taacgatcca attgatccaa
    20641 tttgtagaga acgagacaca cgtacctttc catctaaagg aagaagcttt attcgaaaat
    20701 ctctataagc acatggctgc actgattgta agaatcaaga atcatatcca agtcatgaat
    20761 ccattgaaag aaaacatccg aacaaattat ttcccaatct accgcgcagt cactcatttt
    20821 gttcctacga tcgaagaagt aattggtggc aagatactgg aggacgagat agcttttctt
    20881 gtcatccact tttcgacgat cgccagtgta atcaagcgag acttgaatta tgtttataaa
    20941 tcagtcgttg tctgtaacca tgggatggct actggaaatc tgttagcaga gaatctgaaa
    21001 gaaaagtttc ctcagatcga gattactgca gttttaagtt caaaagaaat cgaattagta
    21061 gataaattgg atgtcaatct ggttttcagc acctttcaat tgaattatcg aaataagccg
    21121 cttttagttg tcgatccgat tttaactgag acgaatatct tggttataga agactttttg
    21181 aaaagaaatg aagaactaca gcggttagag cctaacagtc atgaatcgac ggagttattc
    21241 actcaaataa tagatgtcat gaaagaaagt ggtggccgag tagatcgaat catctatggc
    21301 aagttagaaa aattattcga aacaaacaat ttagaaatca acaaaaggga gatccagcct
    21361 atgttaaaag atattttgac ggacagcaat atcatgatca aagaaaaagc cactacttgg
    21421 gaggaatcga ttgaactagc tgctagacca ttggttaaag aaaacattgt cgaagagcgc
    21481 tatgtcgaag cgatgattcg tgcagtgaat gattacggac cgtatatcgt gattggcaaa
    21541 cacatggcac tagcccatgc ccgaccagaa gatggggtca ataaattagg agtcagcgta
    21601 gctacgattg agcaaccaat cgatttcggt aatcctgaaa tggatccagt aaagattatt
    21661 ttttgtttgg cagcagtaga ctcctactcg catctgaaca tcatgaaaga actaattgaa
    21721 ttgatcaaca acgaagagaa gttggatcga ttgattgaat gcacaaatat cgaagatttc
    21781 aaacaactgc ttttttcagt agttgagaaa taataaatga tacgggggag aacaaagatg
    21841 aacaaattga acattttatt cgtatgcggt gcaggtttag gaagtagttt tgcagcacag
    21901 atgtctgctg aggatgtttt gaacaaatta ggagtagaag ccaaactgga ccattgtgat
    21961 atttcttccg cagtttccat gaaaccagat gtgatcatca cagcagaaaa ttttcgttcg
    22021 cagttcgaaa agttcgcagt tgatcctaaa acggcgatga tctatttgaa aaatattgtt
    22081 tcaaaaaatg aaattgaaga aaaaatcacc ccagttctgc aagataaggg agttatctaa
    22141 atacaaaaag aaaagagggt aaaaa
//
LOCUS       DACDIG010000048        21938 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.048, whole genome shotgun sequence.
ACCESSION   DACDIG010000048 DACDIG010000000
VERSION     DACDIG010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21938
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.048"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            297..536
                     /locus_tag="IV247_001850"
     CDS             297..536
                     /locus_tag="IV247_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777918.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(898..1158)
                     /locus_tag="IV247_001851"
     CDS             complement(898..1158)
                     /locus_tag="IV247_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777919.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1343..1840)
                     /locus_tag="IV247_001852"
     CDS             complement(1343..1840)
                     /locus_tag="IV247_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAP8777920.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(1970..2680)
                     /gene="budA"
                     /locus_tag="IV247_001853"
     CDS             complement(1970..2680)
                     /gene="budA"
                     /locus_tag="IV247_001853"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAP8777921.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(2693..4357)
                     /gene="alsS"
                     /locus_tag="IV247_001854"
     CDS             complement(2693..4357)
                     /gene="alsS"
                     /locus_tag="IV247_001854"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAP8777922.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(4562..5224)
                     /locus_tag="IV247_001855"
     CDS             complement(4562..5224)
                     /locus_tag="IV247_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP8777923.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(5234..6049)
                     /locus_tag="IV247_001856"
     CDS             complement(5234..6049)
                     /locus_tag="IV247_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8777924.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(6311..6754)
                     /locus_tag="IV247_001857"
     CDS             complement(6311..6754)
                     /locus_tag="IV247_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAP8777925.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(6888..7226)
                     /locus_tag="IV247_001858"
     CDS             complement(6888..7226)
                     /locus_tag="IV247_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8777926.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(7214..7591)
                     /locus_tag="IV247_001859"
     CDS             complement(7214..7591)
                     /locus_tag="IV247_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8777927.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            7815..9008
                     /locus_tag="IV247_001860"
     CDS             7815..9008
                     /locus_tag="IV247_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8777928.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(9174..9596)
                     /locus_tag="IV247_001861"
     CDS             complement(9174..9596)
                     /locus_tag="IV247_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8777929.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            9991..10185
                     /locus_tag="IV247_001862"
     CDS             9991..10185
                     /locus_tag="IV247_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777930.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
     gene            10182..10676
                     /locus_tag="IV247_001863"
     CDS             10182..10676
                     /locus_tag="IV247_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777931.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(10838..12010)
                     /locus_tag="IV247_001864"
     CDS             complement(10838..12010)
                     /locus_tag="IV247_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8777932.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(12216..13031)
                     /locus_tag="IV247_001865"
     CDS             complement(12216..13031)
                     /locus_tag="IV247_001865"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP8777933.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(13181..13627)
                     /locus_tag="IV247_001866"
     CDS             complement(13181..13627)
                     /locus_tag="IV247_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP8777934.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(13624..14628)
                     /locus_tag="IV247_001867"
     CDS             complement(13624..14628)
                     /locus_tag="IV247_001867"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP8777935.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            complement(14668..14997)
                     /locus_tag="IV247_001868"
     CDS             complement(14668..14997)
                     /locus_tag="IV247_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP8777936.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            complement(15123..17450)
                     /locus_tag="IV247_001869"
     CDS             complement(15123..17450)
                     /locus_tag="IV247_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8777937.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            complement(18293..18586)
                     /locus_tag="IV247_001870"
     CDS             complement(18293..18586)
                     /locus_tag="IV247_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAP8777938.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(18989..20167)
                     /locus_tag="IV247_001871"
     CDS             complement(18989..20167)
                     /locus_tag="IV247_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8777939.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(20271..20585)
                     /locus_tag="IV247_001872"
     CDS             complement(20271..20585)
                     /locus_tag="IV247_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8777940.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            20692..20907
                     /locus_tag="IV247_001873"
     CDS             20692..20907
                     /locus_tag="IV247_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777941.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            complement(21207..21449)
                     /locus_tag="IV247_001874"
     CDS             complement(21207..21449)
                     /locus_tag="IV247_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8777942.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(21436..21864)
                     /locus_tag="IV247_001875"
     CDS             complement(21436..21864)
                     /locus_tag="IV247_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP8777943.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
BASE COUNT         6457 a         4319 c         3709 g         7453 t
ORIGIN      
        1 attgacgctc ccagctatcc tcctagcaac tatttaaata cccaaaaaaa ttacctctag
       61 ataatatcat acctacgttt tcacttataa attttttcat aaaaaaagcg tttagaggca
      121 taatcgttaa tacagatggt aaccaatgta tctggtttga actgggatat caccctctcc
      181 atatatgtat tactaagact aatagatact ataaaagact tgatagtttt gcattgattt
      241 tgcctaatac tgagcatagg tgtatgctaa acgatactat aaggagtgtg tacataatga
      301 aaagaattct gtttatcagt ttatcaaatt tattgttact tactgcttgt gggacttctg
      361 catcaaagaa cataccttca gatacgcaaa tttcgaatag tatcgattca acaatttcta
      421 caaatgagaa aaattctccg gtagaaactt ccgatataaa agtttctacc tctgcaaaaa
      481 acagggtaaa gaaactgtca gcacgtctcc tttatttcaa acaaactctg aattagcttc
      541 ttcatcttct tcagcaaata atgatgatag tttcgcgttt actcctaact aaatgcaacc
      601 tttacaaatg ctttacgata acaagcctga gctaaatgat cctgatatca gttttgcatt
      661 ttttgaaatg attggatcgg atgatctttt taaaactact agtttgtcta tccgaaaata
      721 ggacagttct ggcagtatag gtttttatcg agtttctcca ccaggcctcg ttacagagac
      781 agattctcta ggcgaacctt ttgaataaat caaaaatcat gaacaatcac tgccaactaa
      841 ataaagtgcc ttcaaaagct tggtcttctc gcttttgaag gcactttatt taaatcttta
      901 tgaaactaaa gtatgttcat atttgttgct atagttatat agatattctg ttgtttccaa
      961 ctcataaacg tgagtatatt ttgccactac ttttccgcat ttttctaata tttcattgcg
     1021 atcgacgtca tgatattcgt ctacgcagat cacaacacaa ttttctaatt tactgattac
     1081 ttctccaacc actttttgtt tcaagccaat aggttggcaa atatattttt ttcctaaaat
     1141 gatagtttcg ttattcatat ttcatccatc cttaatgttt ttccattatt taaaatgata
     1201 ataccattaa aatatgatta tgaaaatcaa taacacttgt gattttataa aaaaataaaa
     1261 aaaccgaaaa acagaaatgc ccactgtctt tccggctctt tttattcttg atttctcttc
     1321 ttttttgttt caatagacga ttttagttat taataaatga aaaagcattt atattaaaat
     1381 ttgctaataa aagatctaca tatttttcta gaaatgcttg atcgatttca tcataccctt
     1441 tcgttactcc gcaatcgata tctaaaacac cgattaaatt accttctttt accattggca
     1501 ctacgatttc tgattgtgcg gctgaatcgc aggaaatgta atttgcatgg gttttgacat
     1561 catcaacgat caatgtttct tgtttttcag ctgattctcc acaaactcct tttcccatct
     1621 tgatacgtgt gcaagaaact cgcccttgga acggtcctaa aatcaattca ttaccatcga
     1681 ataaataata tcctgcaaag acagtctcag gcaaagtctc attcaacaat gcagatgaat
     1741 ttgctaaatt tgctaaccag ttatcttctg cttctaataa tccttgttgc tgtaataata
     1801 aaatttcgta tgcatctttt ttctgttttt ctgtccacat gataaaaacc gctccttttt
     1861 gatctatgga gaagattata gctcagtttt tttgtaaacg ctaggtttcc tcctcaaaaa
     1921 aacgagatac gtcaggttaa tcaccttgaa cgcatctcgc tgtctttttc tattctgctt
     1981 gttggatttc tgctgctaaa tcagcataat caatcgtttc gtgaacaaaa gtcggatcat
     2041 caactggaaa atgctgtaca agggacgaga tgcggctaat ctcaactttt ccttcagcca
     2101 cttcaaaatc cattacatgt ccgccaaatg tatgtgtatc atcgataaaa tgcaagtgaa
     2161 atcccgctgc agcaactcct tggaataatt caggcgtaaa gaacccgact aatgttcctt
     2221 ggacattgct ttgttggaat tctggttgag actctgaaat ttttgctagt cttctgtaag
     2281 gtttttcttg cttcggcatg atacggatgt gcatattttt gaataatcca gttatcttca
     2341 ctgccaaaaa taggttttcc ccggcttctc ttactaatat gtcttttttc aattgttctc
     2401 catctgcagt ttttgagaca gaaaaactag tatctggcgt aaaatctgta attgctgcat
     2461 agggggtaag ttcacttcca tctaattggg caactgagcc atctgatctt ccttgataag
     2521 ctacgccatc taaaaagatg acttcaccat caagcccatg caatgttccc aatcccagag
     2581 ttcctttttc taatatatga gcaatcgttt ctgttccatc catcaagcca gccatcaaag
     2641 ctcctaatgt cccgtgctgg tataagattt tttcagtcat gtcgacactt ccttaataga
     2701 attgatctgg taagattgtt tctcccaatt tctcattatc acggtaatca atcggaatat
     2761 caataattac gggtccttct gtcgcaaatc cttcttctaa agctttttct aattcctcta
     2821 cacttgtagc tcggattcct ttagcgccaa aagcttctgc atatttcaca aaatcaactg
     2881 gaccaaagtc tacacctgac gcccgctgat atttcatttt ttcttggaac tctaccatat
     2941 tgtagtgtcc atcattccaa atcaaatgaa cgatattcaa ttttttacgt accgccgttt
     3001 ctaaatcctg ggcagagaat aaaaatcccc catcgccgga aactgatacg atttgtgtat
     3061 ttggacgcac caatgcagca gaaatcgccc atggcaatgc aacaccaagc gtttgcatac
     3121 cattactgaa taacaaatga cgaggttcat aacttctaaa gtggcgtgcc atccaaatat
     3181 aatgactacc tacatctacg gtaacagtca tatcatctgt tacttttgac tgaagcgtgt
     3241 taataacttc taaaggatgc aagatacctg cttctccttt tgattgaaca atgtcccgct
     3301 ccgttaattc tgcttgaaga gatgctaaat attcttttgc atcttctgac acttgttgag
     3361 gctctaatga acctgtcaat aaatcaagag tggctgagat atcaccgatc aattctcttt
     3421 caggctgcat aaaaggatcg atttcagctg gcgcttcatc gatcacaatg atccgcgcat
     3481 ctttttccgc attccagttt cttgcttcat attcgattgg atcatagcca atagcaataa
     3541 ctagatcact tcgtttcaaa agcatatctc ctggttggtt tctaaataaa cccacacgac
     3601 caaagaaatg cgcttctaat tcacgtgaga tcactccagc tgcttggaat gtctctacaa
     3661 caggcaatgc tgtctttcca actaattgcc tgatggctaa cgtttctttt tcacttgatc
     3721 ctcgcatacc tactaatagt accggtagtt ttgcttctct aatgcgttca gctaggtaac
     3781 ggatatcatc tgcagaagca gaacctaatt taggatcgct caatggcttg atgacattcc
     3841 cttggacagg tgcatccact acatcttgcg ggatactgat aaagctcgcg cctttttttg
     3901 aactcttagc catacgataa gcattggcaa tcacttctga aattgtttct ggatcttgga
     3961 tttctgcact gtattttgtg attggctgga acagtgctgc attatccatg ctttgatggg
     4021 tcaacttcaa tagatcgctt cgttttactt gtccggcaat tgctaaaact gggtcacctt
     4081 ctgctgtcgc tgttaccaaa cctgtagcta agttactagc accaggacca cttgttgcga
     4141 taactactcc tggctctcct gtaatacgtc cgattgcttg tgccataaat gcagcatttt
     4201 gttcgtgacg agtcacgatt aattcaggac cttgatcctc taattcatta aatacaccat
     4261 cgatttttgc acctggtata ccgaatacat aatcaacatg atgattaatc aaactttcta
     4321 ctacagattt cgaaccttgt ttcatttttt cgtccattga acatcgtcct ttttatgctt
     4381 tatttttaat tattagttag aaataatcac aaataattga tacagtttct aaacctgttt
     4441 cattcaaaag tatagcacgt tttatttcat ttgtttagcc atatgtttca aaaaatgaaa
     4501 aagaatgaca gtttttacat aaaaactgtc attcttaatt gaaagtttca atttataaag
     4561 tctacagatc catagttctc atcaatttac gattgttgaa gtaaatcaaa ccaaatacga
     4621 tagaaggacc tagtagaata caccattggg gctttacgaa aaagaagaga aacaaacttg
     4681 tcaatagtaa aaaatctagt aaactcacat accatttttt caacgtatag tagaacgcta
     4741 tccgtagaac ttgatttggt ttcgacgctg gatttctcac ctgaaaatac cagtagttga
     4801 tgctaaagga aactgataaa caagcaagta aaacaaacaa cggaatcaac cattttccat
     4861 aagaaaaacg tataaaaaag aaacaatcaa aaatcattat cacactaact atccaagcaa
     4921 atagccaata aaaaaatccc tttagccaaa attggcgaaa cgtctttatg aacaaacgta
     4981 ctggtgaaaa acttttttct tttgctaatt cttctgtcat acctatcatc acgatgcaac
     5041 ctggaccaat aaaaagtaaa gaaaaaataa aggaaaagac attccttcca tctatcgaca
     5101 aaaggaacgc agctgctata tacaagatat tgacacacca aaaacaaagg ctacttgcca
     5161 atgattgata caaccaataa aagaattcca tataaacatt tttgtcaaaa cgctgctctt
     5221 tcataatcac ctcctattcc ttcattcctg atgaagcgat cccttctaca aaatattttt
     5281 gcatcgctaa aaatataaaa gcaacaggaa gaacagaaac aactgaggca gccatgatta
     5341 atgcgtactc tgcatcaaat agcccaataa acattttcaa ccctaactgg attgttttat
     5401 tttccgtaga agacaaataa atgaatggcc ccatataatc gttccatgta ttaacgaatg
     5461 tgatgatcgt caaactggca atcgccggtt ttgtaagagg taaaatgatc ttgcgataga
     5521 ttccccactc tgataagcca tcgatccgcg cactctcaca taaagaatct gggatactac
     5581 tgtagtattg cttcaacaag aacacaccaa aagcattgaa cgcttgtaat agcaccaacg
     5641 accacaacgt atatgttaaa cccacttttc tcatcataat gaactgcggg atcatataag
     5701 actgccaagg gacagcaatc gtgccgatat atgctaaaaa taaaatatcg cgccctttaa
     5761 aatttagttt agaaaacccg taagcagcaa aactagaagt aaacaactga atcaacgtga
     5821 tcacaacgct taaaaaagct gtattcttaa aaaatgtcac taaaggaata cgagaccaaa
     5881 taaccagata attctctaaa tgccaagttt tagggatcca ttgaatcgga atcgtgaaca
     5941 cttcattatt tgctttgaat gaagcagaaa ccatccataa aaaaggaatg atcgtcaata
     6001 aagctaatac aatcagcaga atcataccca accattgaat aaagggcatg ttttttgaac
     6061 gaatttttgc tttttttctt tcatttcgaa cacctccaaa gttataatct tcatttcttt
     6121 gtaatgatta caatccagaa caacggttat tatagctgat tcatttccgt tcttcttatg
     6181 tttgtaagcg cttatttaac aagttatgta aatctcttta ttttttttga tttaaaaatg
     6241 aaaaaataca aaagcaagac tgtcatacgt ctcgcttttg tattgaaatt tcttcgatta
     6301 gagatactta ttactgttca aaagcttctt tgaaacggtg aatcaattca ttgactacag
     6361 gtgataaaat ccgattcttt ttccagacta gcatgcagtt cgtttcaatc attgggtcaa
     6421 gcggaacgaa tgtcactcgc tttgaaagat gacttacagc accttctatg actaaagcag
     6481 cacctacctg attctcaatt agcgcaaata cattgaaaat caaattgtac gttcctgtga
     6541 tcagcaactg ctcctttttt atgcctaacc agtcagcaaa cattcgctgt atttctggac
     6601 gctttgagat caaaagaggt acattcgcaa agtcttcttt tgttatcacg tctttttttg
     6661 ctaaaacaga attttctaaa atgagcaatc cccatttttc ctttcttggt aacaaaatcg
     6721 tttcgtattt ctctgtatct actggatcta gcaaaatcgc taaatctaac aggctttttt
     6781 ctagtcactc cgtgatatca tcacttgttc cgctgtggat atgaaattga acgctgggat
     6841 aatcacggat caacttttct agtaatagtg ccaatgtatt cgaattatca gcttccacac
     6901 agccgatcgt aaatcggccg ctgaataatt gtttttttct gttctcgaat tcttgagtgg
     6961 tttgattcgt taaatctaaa atttcttctg ctcggctttt ttagaaaata acctgcttct
     7021 gtcaagatca ttttgcggtt ttcgcgtaca aacagttctg ttcctagttc ctcttctaac
     7081 tccttcagtt gtcggcttaa ggttggttgt gtaagatgta gaacttctgc ggcttgagag
     7141 attgttcctt cttctgcaat tgtccaaaaa tattttagta agcgtaactc catagcgatg
     7201 ctccttgatt tttttacttg atacattcca ttttatttct agataacaca cgatcatatt
     7261 tcttgatttt tccttgtaaa cgttccaaac actcttgcat ttcagcaatt tctttcgtca
     7321 aaagttcact ttcttcaatc aaaagcttac gccgctcaaa tacagttgct tctccttcgc
     7381 tagataggcg aatatattca atcaaagact cgatcgataa tccggcattt cgcatacaga
     7441 tcgtaaagtc gacccatttt aggtcttctt ctgtataatc acggattccg ccttttgtcc
     7501 gattaactgg tggaatcaac ccgattcgtt cataatagcg caatgtatcg gccgaaatgc
     7561 ccttttcttc actgacttgt ttgatattca tctgatcact ctttctctta atttcttgcc
     7621 actttactta tgtattttat ttattctaca actttaagtg tactccaagt caatactcat
     7681 ttctattaaa actaagctgg attatctata tcggttgttt gtgtattatt tcaaataaaa
     7741 aagtcttgtt tttttattct tttttggata tacttttaac aatttaggga aattttagga
     7801 aaaggggtat aaaaatgaca acgtatcagc gaaaggtatt attatcgaca tcggcgggaa
     7861 ttgctttaga gaatatggat attatgtttt tagctttttc attatcttca atgattactt
     7921 cttttcatat tagtggtacg caagcgggtt tcatttcaac gatcactaat ttggggatgc
     7981 tcattggagg tatttttttt ggcatcatag ctgataaata tggacgtgtg aaagtatttt
     8041 ctcaaacggt ccttctgttt tcgattgctt ctttattgat gtattttgct tcaaacatct
     8101 acttggttta tctctttcgc tttatcgcag gtgtaggagc gggaggagaa tacggggcgt
     8161 gtatgtcctt gatttccgaa agtttttcaa aaaaacaaat tggacgagct tcttcggttg
     8221 ctggtattgg tgcgcaggtc ggtgcggctt tggctgcgat tttagctgct gtgatcattc
     8281 caacgtttgg ttggaagatg ttgtatgtcg taggtgtact gccggtgttg atggtcttgt
     8341 tcattcgtcg cggcttgact gaaccaaagg aattcaaatc tgtacaatct gccgaaaaga
     8401 aaaccaaact tagcagactg tttgaatcaa aacatcttgc ttggcaaaca tgtggcctta
     8461 gtatcatggt aactgtccaa attgccggct attttgggtt aatgaattgg ctgccttcaa
     8521 ttatgcaaaa tcgattaggg ctttcggttt ctgggtcttc attgtggatg gtcagtacta
     8581 ttttaggcat gtctttgggg atgatgactt ttggcataat catggataaa ttggggccta
     8641 aactagcctt ttctttgttt ttaatttgtt catccttatc tgtctatcta cttgtgttag
     8701 cccatagcca gtggagtttg gtagcagccg ctgtgatagt cggctacttt atcaatggca
     8761 tgtatggtgg atatggcgca gtgattagta gtttgtatcc aacagaaatc cgcgcgacgg
     8821 cgaataattt cattatgaac cttggacgag ctgttggtgg tttttcttct atcgtcatcg
     8881 gtttcttgat ggatcattat agtttaactg ccgtaatctt gtttttgagc ggtatttacc
     8941 tagtcagtct gctggtcttg acaactctta ctggtgttac agcattaagg gaaagcttgc
     9001 agcattgata gttgagtctg taaatgacaa gaaagaggtg tggcaaaagc ttgcatctgc
     9061 ggtaataaga ccagccaaat caaaaacatg agacactgtt tccgtttggc tggtcgtatt
     9121 acttgatgca gggcagcttt ttcaactgct aatttccttc tatctggcgg cgactattct
     9181 ttgacgtccg tcatttcaaa gtactttact aataacttag cataacgaaa aactgtggat
     9241 acactcgtat attgagcgtc tgcataatct tccaaatttt tctcaggatg ttgatacatg
     9301 aattcaatca aatgataatt atatgatttt ctgtagaaat gaagataaat aatgtagatt
     9361 gggaagttct cctgcttttc catagaaaag cgccgattat tctcattctt caaaatgacc
     9421 ttatcaggat atttttggaa caattcttct aaatctgtta gataattcaa gatagactta
     9481 cttgatacct tcaattcgtt ttgtagttct tctattgaac accaacgttc tgtttcaagc
     9541 agtaaatgaa tcattttaat tttcttttga gaaacttttg ttaaaaaagt ctgcatattt
     9601 tctcctcctg tatctacttt actatactac atacaagaaa aaaaacggat acaaatatca
     9661 tattgtcaca ccgtcaatag tgcataaacc tgttattatt tctctttttt tcctaagtat
     9721 tctattgata tgaatcattt aatcgtttat tatataaatg tcaataatct aataataaaa
     9781 taacgtacat attttattat tgcatactta ataacagaac tatagcaaaa aatcccgtgt
     9841 atcaacacgg gattagatta agtttcctca atcactattc ataacacaat tattataaca
     9901 agtaagtaaa caattgaaaa ggacatttat tgaaaacggt tgttttttac ttaaggagga
     9961 aatttagcga aaaaattagt tgcattgttt ttggtcgcag gaagtttgtt gagtgggtgt
    10021 ggtgctacta cgtctactac tcaaagcaca agttcagatc aggcttccag cagcagtacc
    10081 acgatctccg aaagtacggc agattcatcg aaagaacaag aacaaattga aaagaaaaaa
    10141 gaagagaaac aaaaagcgga aaaagctgca ctagaaaaaa agtgacagaa gcggaatctg
    10201 ctttgtcaca agctgaagca aatcctacaa aagaaaacta tgcaactgct tcagcagcta
    10261 tccaagcgct acctgaaaac aaacaagagt tgacaagccg tcttgctgca attgattcca
    10321 caatcaaagc aaaagaagca gaagaagaac ggaaaaagca agaagaaatt gcacgacaac
    10381 aagcagaaca agcgcaacaa gaagcaatcg cacagcaaca agcagaacag gcgaggcaag
    10441 aagcaatcgc acagcaacaa gcagaacagg cgcgacagca atcagcagct gaacaggcct
    10501 ctcagcaggc acaagccgca gctcctgagc aaaataatga acaaactgtc tatgtcacac
    10561 cgaccggttc aaaataccat acacataaat gtggaaatgg tacgtatacc cctgccactt
    10621 taagcgaagc tcaagctcgt ggtttaaccc cttgctccaa atgttatgga aactaattca
    10681 aaagttatac tataataata aaattcttct gaaacataaa aaaatagaag atggcgttcc
    10741 agatagtaaa ccaaaaataa cagtttacac gtccatttcg tcagcttctc tcttttttca
    10801 tttagtctgt tccctctgct tttttggaca tcttcattca ttctttctca atcaaatagt
    10861 acacctcttt ttcttttcgc agtttatatc ttcctttttt caatttcatt gaaaactgtt
    10921 tcgaatgttt atgttttaca tggattttaa gcaggagctc ctcatgttgc aagcgatagt
    10981 tcctgccttt taaatcagaa aatctcacta gtccgtattc atcagaagct ttcacaaatg
    11041 gaactttttc tctagtaata tagtgtttgc cgttgagcgt ttttacagaa aagaccattc
    11101 cttcgattgg tcgtccgttt gtgttcttta cataaaaaga taaaggatag tttcgcttag
    11161 cgatggccaa agaatctaaa taagcgatga gtagccaaac taagccgatt gatccaagta
    11221 gtataccgat aaaccaaata aaaaatttcc attttttcca ttgatcaatt ccttggatcg
    11281 ctttttttgc ctcttttgct tgataaggaa tacgatgccc cgtcacaagc agtcgatgtg
    11341 tattgaccat ataaggagta caggtaagca aagtcacgag atctctgcct ttttggatac
    11401 ccaaagaatc tacttcatca ggtaagacca cttggatttt ttctacttga tacgccaatg
    11461 tctttccatt gatctggatg aaaaactggt ctcctttttt caacttaggc aaatctgtaa
    11521 ataattttgc ttctgggagt ccacgatgcc cagaaagaac ggcatgggtg tttttcccgc
    11581 cagtcggata agaactttct tccaataatg acgttccttt ttgcaaaaat atttccgttg
    11641 tctgatcgaa tataggtaag ctgacattga tttttgggat ttcgatgact ccaagcatat
    11701 gttccataaa aaaggcatta ctaggtaaat cttttaatgc ttttgcatca actgcttggt
    11761 tgaaactgcc tatccctggt gatacgtttt gctctgccaa ctgttggttt ttctttgtca
    11821 ttttttcctg ctgttttttt atttcctctg aattcttatt cgatgcttgc ttttgataat
    11881 ggatgatcat ttgctgggca agaaaatcat tcagtgaatc ttctacaaaa ggataagaaa
    11941 agatcccgat cccaacgact aaaatgattg ccatgaagat gtccagtatt cttattctgc
    12001 ttttattcat catgtccacc tattttctaa tatgtatatg aagaggttgt gaaatcagcg
    12061 ccttgacctg agtattagcg aagcaaacgg caaaatagtt cctcctattt tgccgtttgt
    12121 tgagcgaata ccaagagacc gaccacttgt cacaaccttt ctccccattt gaatcactat
    12181 cgtttattta tcgtatgatt tttgtttcca atagtttaag cttctgtttc ttgatggcgt
    12241 tttttgaaat agatcactgc tcctccaact aaagcagtac ctaccaagat gaatacgatg
    12301 atccccatac caccagttga aggcaagatt cctttaggtt ggttatcgat cgttgtttca
    12361 tcagaagttg ttggatcatc atctactgta aattcagtgt acgcagcttc attcttaggt
    12421 aatgcgtagc cttctggtgc ttttgtttct tgaaggcagt atgccccttg tttcagccct
    12481 tcgattgaaa atttcccttc tgaatcagaa atcaatttca cttcttccca gtcagaacct
    12541 gtgacttcat ctgaccaagc atatgggtat tctcctgagg caaggtttgc tatttttttc
    12601 attaatttaa cagttggatc ttttgctctt agaatagaaa attctgcatc ggctaatttt
    12661 tcttgcgtgt tcacatcttg tttgatgaag tggaaatcac cgtagttttg gacattttct
    12721 acagtaatcg tgttatccgt agttgtattt tcccgattga ctacaccgtt tggattccca
    12781 agattggctt catctgcgcc atggatgata ccggttgttt gttgcaattt tccgtttttt
    12841 actataaaag tagtttcttt ttgtggattg gttaattgat aaccatctgg tgcttttgtt
    12901 tcaactaaag tataattgcc gtctattaat ccattgacac tgatggatcc ttcagtatca
    12961 gttgtgaatg tcaacgctga ctcagcagga tcatagagag cgacaccagt gttttcatca
    13021 aaaccggtca cgaattgatt ctctgaattt ttgatctgga attgcgcccc tgctaactta
    13081 tctacccctg aaaagttccc agctttctgg attttgatat tgctttcttt gatgacattt
    13141 ttagcatata ggtgaatcgt gtctaaaggt ttgtcagggt ctagggtaag cttaacacga
    13201 tatttggcga cgtttcgaca ccgggctttg gtgtttctac aaagacgtat acagcatctt
    13261 tcccatcaga tttacctgga agagtcaggt tagctaatcc ttcaccagaa gtcgttacac
    13321 ttgcgaaggg tgtagcgtct gctggaactg ctgtgtcttt acttggatag tacgtagcag
    13381 tgatgtcata tacgttgaat tcaacgccgt tcaacggttc gctattcgga aatgtcaatt
    13441 ccgttccgtt gtttatttga ttttctggtg ctgaagtgaa tttcaattta tgcaaattaa
    13501 ccgtaacatt ttctggtgta ggtgatacgg ctgcagaaac ctctgcagtt cctaggccga
    13561 gaacgaaagg aagcagcatg atagcagttc ctaaaaacgt ccatattttt gttttgatgt
    13621 ttttcatttt atttccctcc tattgtttcc tttttgttgt tttttataaa accaagctgt
    13681 ccctgttgaa aggactagca ataaccctaa aattgagaaa cctccaagca tctcttcgct
    13741 tgtttttgga aagcttcctc catttttttc atttgatgga ccatttgtct gcatctgaac
    13801 tggcgttttt ccattattcg tcggatttgc ttttggttcg ttgatgattt ccaatggtat
    13861 aggatgaagc ggatcataac tgcctttttc aacttcaaag agaatggttg attgactcaa
    13921 tatatatcct ttaggcggtt taatctccac tagttcgtaa cttccaaaag acaatccatg
    13981 gacagaaaat gttccatctt tactggaagt aaaaatcgtt gctcttgaat catctttggc
    14041 tacccaatga tttatcccgt tttcttgcgc taagtattgg tcattttttt tgactagaaa
    14101 ttgagcatct gctagttttt gtttcgtttg ccgatccact ttgacaaatt gtttgcctcc
    14161 tgtttggatg gcaagatgtg tttctactgt ttgattgtca gtcgtcaaac gtacatcgtt
    14221 atcaaatgtc gttttttcac tgttttcttt caatgtcatt tggtaacgga tcgttaattt
    14281 ttttccagca aacgaactaa gatcttttcc agaatctgat aagacaaaac catgttttat
    14341 tttttgtgtg tgaaatgtat gaatcttgtc tccaccaatc aaaaaatcga tcgtttctgg
    14401 ttctagccat aactgaggat cagctgtatc tttgatcgta taggttccta tctcgttgat
    14461 gttctcagga attgttacgg tgatgagata agggacttta tccccatctg caaaatctgt
    14521 ctgcttattc tctactgttt ttgttaatgt gagtgcagca ggtatttttt gttcgctctt
    14581 tggatacaag tggatggtcg tcaatttatg attagatgaa tctgtcacag gaagcacaac
    14641 gacaaatggt gctgattttt cttttactta atctgaagtc tttatttccg taaatcggta
    14701 aacagcgtca cgaccttgct catctttgtc cgatgcacta aaagaagcaa tgccctcttc
    14761 ctcgtctact gtttttgtca cattttctgc cagtattttt tcactatcac ttttttgtgc
    14821 tagttttctt tgtgcgtctt ccacactact tccttctgac cgcagcttgt aaaattcatc
    14881 cgagacatca tatatagcga aagtaacgcc gttcaaccct tttactcctt gaaagttatc
    14941 cgggatattt attccgtcgt tttgactgat acttggcatc ttatcgcttt cgaacattcg
    15001 attatgcaaa gtaaatgtaa taggtgattc tctagctgac acagtcggag aacctataaa
    15061 aaagaccagt aggataaaaa agagaacagc aaatatatcc acacgtacat tttttgtttt
    15121 tttcatcgct gttcacctgc ttttttctta cgatggataa aataaaagaa gccagaaaaa
    15181 ataaagatca gtcctaaaaa gacaaagggc agtagaccta tcccacctgt cgaaggaagc
    15241 aagagagtcg gattgatcgt caacttagcg gtatctgttg cgataatcgt ataattattg
    15301 tttacacttc cttcgcctaa actaatcgtt actatttgtg atccaatatc tgtccggctg
    15361 ttaactgtta gggtataatc caatgattcg cctaagacaa caccggtaac gtggagattt
    15421 ccaaccgaaa tcgagtgact ttgggcatca taattaaagg cagcgtcatc gacagtaatc
    15481 actgcgttag ctttatagat ggtataagtt cctgatgtgt tggctatatc agtaaatgac
    15541 aacttgggat ttgctgcttt gactttgtct attccgctat tcgttagttt aattgtatag
    15601 ctgccagcat taaccatatt ttttgctgat tgtgcatctg ctgtgtaatc catactgcta
    15661 agtgccactt gtactggagt aagcgaacta gtgacaccct ctgctagctt gacatggatt
    15721 gacgggacac ctgcttttcc atcatatgtc ttactgccac tgagctctac cgcaccgctg
    15781 atttgcttaa tcgtatagac atcagatgtc tgcacgatag taaataaata gtttgttctg
    15841 gcagctagtt tagctagtcc gctttttgtc aattgccaag acgtataggt tccaatttca
    15901 gtaggcgcag ttacttcaac tcccttttca ttcatcgctt tgaaatcagt tggattccat
    15961 gtgatttgat caatactgag atcattgttt aaaagggatg ttgattctgt tttatcatta
    16021 aaggcatatt taacgatcgg tacaacagaa agaccatcat agtacttact gccatttgca
    16081 ttgattgata ctgttgcatt tgtcttataa tataggatga cttcagaaat ctgttttcca
    16141 tttgcgtcct ttacgattac atttccattt gtatctaaac tagctaaaac actcattgct
    16201 gattggttct ttgtttctat cgctgaaaca gctgtaaagg tatatccgtt gatagtaggc
    16261 gtattagtcg tcaggtcttg tgcagcactt ccgtaagtaa gattcattat tttagaagga
    16321 gcaatcatct gtccaaaata gtcttgatac ttaatagtga ttggtgtttg tttgcctgta
    16381 taggtcaccg tatcgacgtt actttctagg gctgtgaaag aaccgtttgt ttgtgcaaaa
    16441 gaatagcccg ttactgctgt tggagtgata accactttgt cacccacatg gattccagat
    16501 aatgtagtcc cgtacgttcc gatttttgct gctccaccat tgactgtata agaaaaagtt
    16561 cctgtccgat tcgcattgac ctgaaccgtt gctgcttgat aggttggaaa atttgaaaca
    16621 tttcccttac catcaacgat ctgcccattt ttgatcgagt accccgtgaa tggaatatca
    16681 tttgctaact gtataccgat tgaaaaagta ctcatacctc ctccggcagg aagccctccc
    16741 catgttgagg gtgtaaaaga ataactttta tccgcattgg gtgtaactga atcgatcgta
    16801 tttccagcgg tagccacccc catatagata ctttttggga atgtgatagt agtaccattt
    16861 actgtggagc caataaacag attatggata tctggacggt tatcaataaa agcgattgct
    16921 ttggttacgc cttgtaacgc agttttatcc gcactcatcg atccgttaac cgcaacaatg
    16981 actccatctt tggcatatat cactgtatat ccatctggta tggtatgtcc gttgaaattc
    17041 gcagtggccg caacagtact aaatgtctgg cttctacctg tttctgaata agctttctcc
    17101 cctgctgctt ttgctgctgt gatttctttt tctgtcggat caatcagttg agtcgaggat
    17161 gtggttgagg ttgtctcgtt gttgctagac tgattggtgc tttctatgga ctgtatttca
    17221 gaatagggtg gactatttgt ctcctctgtc gtttggttag caaaatttga cgtttctgta
    17281 taaacgttta cataaatact tgataagctc cctaagaaag taaaaatcaa gatgatcgaa
    17341 atcataatgg gatgtaagta ttttctttta gatacagctt tgagtctaga aacaaaatgt
    17401 ttttgacata tacgttggat tttattttga gttttcttaa tcattttcat ccctcctctc
    17461 aataaaaaat catcaaataa tcctttacac gttattctaa atttaatttt tattatttta
    17521 ttgatgtata gaaagtttgg ggcttttttt ctctttttat tctaaggtta ttttagtctt
    17581 gtcctttaat acaaagattt tatttttaaa taaaaaatac tcttttttta cgattattga
    17641 aaaaaaaaga taaaacttga ttaattgaag tattgcttaa atttggaagc tatgagggaa
    17701 gaataaaaga gttaggacgg gattatctac tatttttctt tgttttaaat atttgttatc
    17761 tttgaacata aaagagaaat gtgtcgactg cagtgttgtg tccctcatca cgaataaacg
    17821 gtgttcaact agctgatctt gtattcactt aaaaaatggt aaagtacgag ttgctattcc
    17881 ccattttcaa ttttctaacg ctcaggacaa aatactgttt tcacaacctc attggaataa
    17941 aagcatatat attgtttcaa gaacgaaatc ttgttatctt tttaaagggc atctgtccaa
    18001 aatctaattt taggaggtac gttttggtat ttattcgtac aaataaaaga gaattatctt
    18061 tattcatgta acgcatccaa ttgatttttt catgtacgct cctggaacga ttttactgac
    18121 gattgcttat ttaactgcga atcgctcatt agcttttatc atagctatcc atattttgaa
    18181 taacgttctt ggctttgtat tatagataca gatgacaaaa aaagttcaag tatgcggtgt
    18241 agaaccaata tcgcatactt gaactttttt caatgagtat aaattaaaac ttctaatttt
    18301 caattcccat catctgatat gcccgaccgt ctgaacttct tttgaaaaag ccaaatgtga
    18361 ttagataacg tctttgttcg acataatcgc cactagaaaa tacgttcagc agtatcgaat
    18421 taacctctgt ttctgtataa gtcactccat ggtcaaagcg tttcaagact tctttcaata
    18481 ggatgtattt cttttttgtt gaacttggta cttgttttac ttttccatca ttcatgaatg
    18541 tgtctaaaac tttcatcttt tcttgctccg tataagtata aaccatcttc tttctccttt
    18601 tgattctaag aatactccat ttatgttaga atattctgga ttttttatta aagcggttac
    18661 tcttaagaaa gatattatca tagtgctctt ttttttacta ctacttatag gtagatttat
    18721 tctaatccat tattctcccc taccaataat taaagaaaaa actggatcag ggtgttcaaa
    18781 ttacatgcta agaaaaaaga acccttgaat accaagggtt ctttctcagt ctgataacaa
    18841 tgattaacga cggatttctt tgatacttgt gaaaagtcgt taaagtattg atataaaagg
    18901 atttttatta cctctgacta catattgact aatttgttaa aaaatcgttt ttcaagaatc
    18961 ctgtaatacc agtgctttca ccgttttttt aactctctaa agaactaaaa gcaatcagag
    19021 gttttacctt ttcatttgac gttaagtggc gataaatttt cgaagttgtt tggtcagtat
    19081 ggcctaagcg atctttcaag acttcccagc tacagtgttc cgctactaag cttgccattg
    19141 tatgacgagc taaatgagta tgaaatggct tattaattcc tgagcgattc acgcaccgtt
    19201 tgacgtgatc atttacatac tctcgacgat aaggaacacc acgtttacta gaatcaagat
    19261 acacaaacaa atatgtttct tctataataa acctgtaatg ttgcctcatc tcacccaaag
    19321 ccttaatcaa ctgttgtagc ttttccataa ctagaggagt tactagaact cgtctagcag
    19381 aactaactgt cttagtagtt tcaattcgaa aatccttact cttaccttta actgccgttt
    19441 gcttatagaa cagaatttca tttcttttga aatctatatc tccaggctgt agagcaagcg
    19501 cctcgctgac tcggcagcca gtgtaaaata gaacatggaa aagtacagac atcggtaatt
    19561 cctcatgaac aatgccataa ttcaaaaaag ttctcaattc atcgacagta agaaacttgt
    19621 cctcaatatt atttatggct tcttttaaat cagttaccgt taactgatat tttggcattc
    19681 tcacagactt cattggattt tcacttatca aattctttct ttgacaatat ttaaaaatca
    19741 tatttagaac ttgaataagt gattgtaaac tatttttggc atagccaaca tttgttccat
    19801 taacgccaca ttcctttaag gtaaaaagca attcttgaag gcactctggt gtcaaatcag
    19861 acaacttttt atcaccaatc catcgaacaa taatttttaa ttgttcctct cgcttatcat
    19921 aggttatttc cttttgttca tttaacgaac ggtaatattg gaaccattgc tcacctacaa
    19981 cactaaactt gtcattgctt gcaacaattt ttttcttaag cctttgttgt ttctccattt
    20041 ccagataagc gagttgcgcc tcacgttttg aattaaagcc acgtcttgtt gttcgatatg
    20101 cttgaaagta ccatgtgatc ctaccagtag aactgatttg cttcttgaat cgatctgttt
    20161 tttccattta ttgattcctc ctattttgaa ggaacccaac aagcagaaac cctttggaaa
    20221 tctttctatc tatgtcatca ttgtaccgca tcttagaaag aaaatgtaac ttataaagat
    20281 cgaagtagtc gcaatttttc tcgatagtca ttcgaatcac tctttctcat ccattctaaa
    20341 acatcatcac gaaaatagtg tcctcgaatc ccgtaccaac agggaggaaa ctcaccaagt
    20401 tttactaaac ggttcagtgt actatcactt atctgaaata gtcgctttaa atcagtattt
    20461 gttaatacca gaggtaaatg actgactgca agcaattttt ccgtgtaatc ttccagtttt
    20521 ttttgtaaaa ttgaatagaa ctctatttcc agagaattaa catttgtttt aacttccaac
    20581 tccatcaccc ttctctctca aaatcaaaac gaccaccacg gtacccctta aaattgtaaa
    20641 aaaacactcc agtactccat caaaacggac tagaaaagag gaatttttaa tatgaaacaa
    20701 ggaattgcag acataaaaat aattaaagaa attcttgaaa aatctacggc taatgcaata
    20761 gcattcggaa caggaatcaa cttgagtaca gtaaaaaaac ttaaaagtgg agagagagct
    20821 gaagaaaaat taaatcttgc cgatgctatt aaaatcacag aatttggcat gaaaaatatg
    20881 ccaaccaaaa tcgaaatatg gaaatagtag tttttctgat ctatcagctg ttttgccaaa
    20941 gcacatagga atctcacctc cccccagtaa tggcgggccg atttggttac ggaagtatca
    21001 ttatcgtagt cgcgattctc atattaaaat ttgtcgctta aaagctaacc ttgtcatggc
    21061 gtaactagag aatcaaaatc tacatgtact gatagatttg ctattcattt gtcaaagagc
    21121 gctaggctat tttcagccta tggaaatcct ttattttgac ccgataaaaa acttccatag
    21181 aatgaaaagg aaagggaaaa aatactttaa cgagttgtat caaattcaag tatcttctca
    21241 atcaattttg tttccagttg tctacgcata tattcatcta catacataac cattcctttt
    21301 tcttcttcat aaactggttt catggctaac cgtgaaatat aattttgata ttttgacaga
    21361 acgatggaca tggcaattga atcaccagaa gctgcggctt tgattgtcgt tagtggtatg
    21421 aatggtatat atcgtttatt ttgcttcata tttgacaccc aaatactttc ctagctcttc
    21481 caaagccatt tgtcgttggt accaaacact gccgagactt ttcccaataa tatcgccaat
    21541 ttcacgatca ttaagaccga tgaagaagga cataaaaatg atttcttgtc tttgactagg
    21601 taatgctgca atcgcatttg ccaactcctc attggaaata aagattttca taccaaggac
    21661 aataaaagta ccactactat tcaaatttgg ttccaaataa tagaactctg tacctaaccc
    21721 tttttgttcc aatatttcca ttgatatttg ccgttggcga aatctagcgt acttcttttg
    21781 aatatttaac gcctcattgc agactacttt cttacaaaaa ctgtcaaacg tatgttgaat
    21841 gtgggtttcc tctgagtcgc ccataatatt ctccccctca aaaaattcat tcaactcgtt
    21901 ttacttctat ccctttccat tattactaca atctagga
//
LOCUS       DACDIG010000049        21913 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.049, whole genome shotgun sequence.
ACCESSION   DACDIG010000049 DACDIG010000000
VERSION     DACDIG010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21913
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.049"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            125..679
                     /locus_tag="IV247_001876"
     CDS             125..679
                     /locus_tag="IV247_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8777944.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(852..1325)
                     /locus_tag="IV247_001877"
     CDS             complement(852..1325)
                     /locus_tag="IV247_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8777945.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1337..1873
                     /locus_tag="IV247_001878"
     CDS             1337..1873
                     /locus_tag="IV247_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP8777946.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            1913..2284
                     /locus_tag="IV247_001879"
     CDS             1913..2284
                     /locus_tag="IV247_001879"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP8777947.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            2568..3839
                     /locus_tag="IV247_001880"
     CDS             2568..3839
                     /locus_tag="IV247_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HAP8777948.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            4084..5109
                     /locus_tag="IV247_001881"
     CDS             4084..5109
                     /locus_tag="IV247_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8777949.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5403..6512
                     /locus_tag="IV247_001882"
     CDS             5403..6512
                     /locus_tag="IV247_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP8777950.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            6595..7491
                     /locus_tag="IV247_001883"
                     /pseudo
     CDS             6595..7491
                     /locus_tag="IV247_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378728.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            7507..8331
                     /locus_tag="IV247_001884"
     CDS             7507..8331
                     /locus_tag="IV247_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002579255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8777951.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            8363..9673
                     /locus_tag="IV247_001885"
     CDS             8363..9673
                     /locus_tag="IV247_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HAP8777952.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            9821..11080
                     /locus_tag="IV247_001886"
     CDS             9821..11080
                     /locus_tag="IV247_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8777953.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            11090..11962
                     /locus_tag="IV247_001887"
     CDS             11090..11962
                     /locus_tag="IV247_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8777954.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            11974..12804
                     /locus_tag="IV247_001888"
     CDS             11974..12804
                     /locus_tag="IV247_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8777955.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            12844..15027
                     /locus_tag="IV247_001889"
     CDS             12844..15027
                     /locus_tag="IV247_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP8777956.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            15088..16308
                     /locus_tag="IV247_001890"
     CDS             15088..16308
                     /locus_tag="IV247_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HAP8777957.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16305..17765
                     /gene="gtfA"
                     /locus_tag="IV247_001891"
     CDS             16305..17765
                     /gene="gtfA"
                     /locus_tag="IV247_001891"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HAP8777958.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            17842..18948
                     /locus_tag="IV247_001892"
                     /pseudo
     CDS             17842..18948
                     /locus_tag="IV247_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            19270..19479
                     /locus_tag="IV247_001893"
     CDS             19270..19479
                     /locus_tag="IV247_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP8777959.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            19472..19621
                     /locus_tag="IV247_001894"
     CDS             19472..19621
                     /locus_tag="IV247_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAP8777960.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            19656..20440
                     /locus_tag="IV247_001895"
                     /pseudo
     CDS             19656..20440
                     /locus_tag="IV247_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922412.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(20724..21809)
                     /gene="xerS"
                     /locus_tag="IV247_001896"
     CDS             complement(20724..21809)
                     /gene="xerS"
                     /locus_tag="IV247_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAP8777961.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
BASE COUNT         7448 a         3300 c         4212 g         6953 t
ORIGIN      
        1 taaaatcata aactaaatcc cttgatttta ttgactttca aaagtttata tatataagtt
       61 aatgattatg gtaaaatata tacataatca ttaactcaga atatttttaa gaagggaaac
      121 aatagtgagc tataatgtac aaccgttaag gacaaatgaa gagatacaag attttcagtt
      181 ttggctaaga agaacaagta atccggaaag agatagtttt ttatttttgt ttggaatcaa
      241 taatgggtta cgtatgtcag atattatagg attaaaagtt ggagatatac gaggcaaaag
      301 taagcctatt attgttgaac ataaaacagg taagcgaaaa ccgattttta tagataattt
      361 gagagaagaa attttattat atactgaagg taaagaagaa aatgattggt tatttccgag
      421 tcggcaacaa ggtcgacata taacaaggga tcgtgtctat cagatatatg cagatatagc
      481 agaaaaatta ggacgagatg atataggaac acatacatta agaaaaactt ttgggtatca
      541 ctactacaag aaaactagag acattgctac cttaatgttt atttttaatc atagctcgca
      601 agcaattaca aaaaggtaca ttggtataac tgaagatgaa ataggagcat cacttcgtgg
      661 atttaaacta ggtgtataat ttaaaaattt ttataaacag taaagacaaa gattaaaagc
      721 tatttttatc tagaacgatt ttatttcata aagttcgcgg aataaaagct ggggttactg
      781 gtacttggta taggttcgtg ttatataaac taagtaatta aataacattt ttaggttgga
      841 aaaggatgaa attaaaaaaa gagaatcaat acggtctcaa aaacgcaaac gctgcggaat
      901 aagaaaaata ctgataactc aacgtttagc agactatcta atagggatag tctgcttttt
      961 tcgtttttga ccatactttt gaccttctgc aaacttcctt gcttttcctc tgtagaactc
     1021 atgaaatctg caaaacgaat tgccgtttcc tctgtctgct ttttcgcgat atgggtgtat
     1081 atatcatcca tagtggtttt aatatctgta tgtccaagtc gaacatacac ttctttgaca
     1141 gacgcaccag attcaaagag cagactacaa tgggtatgac gaaaaccatg aagtgtgata
     1201 tgttttaagt tattctcttt aatgatgatt ttcaaattgt gatttacata gtctaaataa
     1261 aggggcttat tttcttctga tgaaaacaaa agttgttcac catcttttat aggtttgatt
     1321 ctcattttta gatattatgc ccgcacgtat tcatcacatt tctattgtga atcgctttat
     1381 tcgaccgaca tttgactttt atcataatat actcggttta aaattactaa tgaaaacaat
     1441 taatcaagac gatcatacca tgtatcacct gtttttttca gataatcacc accgtgtggg
     1501 aactgaactg actttttttg aagtacaaga agggaacaac cagttttttg gaaccaatac
     1561 gattgaacga acgatactaa aagttccttc cgaagcatca ttgcattttt gggaaatata
     1621 ttttgaaaca caaggcgttt gccattatgg aatcgaatca ttttctggac gacctatctt
     1681 acgttttgaa gggccagatg caacacgatt agctttagtt cccttacgcg aatttgaaga
     1741 tattgatgat tatttcccgt atgtcacaga ccagatccct acagagcatg ccattttagg
     1801 aattgatgcc attcaactac gcgtacaata ttccgatgcg actgaacgag agttattgtc
     1861 ggtaggctgg tagcacaaag aatacgtcag tttcttttcg ggtcatcaac gagtgacaat
     1921 cttagaaaat caagacactc agttctatca agaagttcag attattcaag atcgtgagaa
     1981 tccagttgcc attgaaggga ttggtagcgt ccatcatgtt gcgtttggcg tagaaaataa
     2041 atctgattta caacgcatcg acaaacaatt acaagagcgc aattttataa attcaggaat
     2101 aaaagatcgt gaattttttg tatcacttta ttatcgtgac ccaaatcaat tattgattga
     2161 aattgctaca ggggaaggaa acctagatgc aaaagcgtat gaaaatcaaa gtccacgctt
     2221 tgaagaaatt cctttatttt taccgcagta cttaaatttc attcgtgaac aagtggagca
     2281 ataattagct aatcaaatcc atgacgaaga gtaagaggtt gactcataag tcaaaaaata
     2341 aagaaaaacg catcaaatcc attctaaaga atgttgattt gatgcggttc ctatttttat
     2401 aaactatcat tttcgactaa taaaactact tatgagtcag cctctctttt tttcttgatt
     2461 tttatacctc tttctaggta aactaaacat ctgccaaaaa taccgtgtct cattgaaaca
     2521 ttctcaacta tttcaaaata aaggggagta ccaaatgaac gagtatattg aaattatcca
     2581 cgcaagagaa acaacttaaa aatgtcagtc tcaaaattcc caaaaataaa attagtattt
     2641 ttaccagcgt atctggttca ggaaaatcat cgattgtttt tgatactatt acccaagaag
     2701 ctggtcgtca actgaatgac acctatgata gttttacccg tttattttta cctaaataca
     2761 gtcgtccaga ggttgatgag attgataatc tttctacggc gattgtggtc aatcaaaaga
     2821 agattggtgg cagtgcgcgc tcaacgttag ggacgattac taacatcaat gcgttatttc
     2881 gtgtgttgtt ttcatgtttt gcaacaccta gtttaggata tgcaaatgct tattcattta
     2941 atgatcctag tggaatgtgt tctgtctgtc agggaatcgg taagacgatt gcatttttaa
     3001 taggattgct ttatttttcg gctattgata atgctgattt tgtattttct gatccattta
     3061 ccgcagaagt tttgtctgga attgaggaac aggcaaagaa tcatggttat tttgtattgg
     3121 tgcataatgt taattcagca gaagatgtta aaactattca gagaaattgg cgttttgagg
     3181 ggttcattgt tgttggtgta cgtgtaaaag aatttttgga attaaataac gtttttgatg
     3241 gtcctgttgt ttatattgat acgcatcttt caaaagagac attaagagaa cttgaacaac
     3301 aaatgaatcg tttatttgta aatacaaatg attaccaagc tagtacattg gcagtggaac
     3361 atttgattca ttatggtcat gagaaaatag cgtttctttc ttttgactac attgtaggtg
     3421 aaccaagtgt tattcaagaa cgacatcaag cttatatcga tacattaaag aaatatcatt
     3481 taacctttaa atcgcattgg atatataaca actctgaatt tgagcgaatt tatcaagaat
     3541 tagctgaatt tactgcaatt gttgttactg gagattattt agcggctaaa ttagtaaaat
     3601 ttttaaaaaa taagaataaa cgagtgatgg aacaattatc gattattgga tttgatgata
     3661 ttacttttgc agaattaatg gatcctgcgt tgacaacaat acgactaaat ccaggtaaca
     3721 agggggcgat tgcttttcac caactgattg gactggccaa acaagaagaa ataaaaaatc
     3781 aagtactact attagaggga gaattgattt ctcgagactc tgttcacaaa atcaaataaa
     3841 aaagctctgt gaaataagta tagttaacaa taaaatatcc gaacgataga gagaatgaac
     3901 tgtgatctag cagttcctcg tcgcaaattc actagaatta gcattattta ctctaaattg
     3961 ttcggatatt ttttcttgtt tttaattatg attacagtgc ctatttttaa cgaaatttgc
     4021 ttattttcaa aatgtatgat tatatgttag taaacacttt caaatatgaa aagaggagct
     4081 attatggtag gaattaggga tgttgcaaaa cgggcgggtg tgtccattgc aacagtatcg
     4141 cgcgtgctaa atgaggatcc gacactttca gtaaccgatg aaaccaaacg aaagattgca
     4201 gaatcggtta atttttatca ttatgtcaaa aaaagtcccg ttgcaaaaaa tttaacaaat
     4261 attgcattaa taacgacagt ttctgaagta gatgagttgg aagatcccta ttttcgaacg
     4321 attcgcagag ggattcaagt cgaagcagaa caaagcaaag tcaatttgaa gaaaattatt
     4381 cgtttgtcag aaacaccgtt ggagcaagac gatttgctaa aatgtcaagg tgtgttgatt
     4441 attgggcagg tgctatcttc tgtgattgaa gaagtatcag caattaattc gaatgtcgta
     4501 gtgattgatg accctagtat tgaagctgaa gtagatgcag tttatactga tttaacaaaa
     4561 gcaacgaatc agcatttaga tcgattgtat aaaaaaggac accgaaacat tgcgtttatc
     4621 ggaggaggac gtgttcgttt agatagccaa ggaaataaac acgtgtcaga tgatgattat
     4681 cgaaaaatag cctatgagac gtggatgaaa caaaaaggat tagaaaaata tagtcaaatt
     4741 tatttagaag gctggaccac acttgacgga atgcaggcag cagatttact tttgaaaaaa
     4801 catgctaaag ataagttgcc gactgctatc atggtaggaa atgatcctat tgctgtgggg
     4861 gtttatcgtt cacttcaaaa aaatggtcta catattccag aagatatttc gattgtgagt
     4921 tttgataata ttgaagttgc tgaattttta acacctgcat taaatacggt gaatgtgaat
     4981 acagaagaaa ttggaaaact agcagttcgc atggttaatg aacgaataaa agaagttaga
     5041 aatgtttcga tcaaagtgat tgtttctaat aatattattg ttagagaaag tgaaaaaaat
     5101 atcgattaaa aaagtaaaca tccaaaaaag ggtgtttact tttttgttta ctaatatttt
     5161 tagtaaaaaa tctcgacaaa acattaagta aacagttatg gtgttgttaa caaaaagaaa
     5221 acgcattcaa aacaggagga attaagatga aacatttcaa aaaaatagct ttaacaggga
     5281 tagctgtttt aactgggaca ttactcgcag catgtggtgg gggagatagt aattctaaag
     5341 acgggcaagt aactttaagc tatgcgagtt gagatactga ccaagctcca ggactaagaa
     5401 aaatgttaga tgaatttgaa aaacaaaatc ctgatatcaa agtagaaatg gagacaacgc
     5461 cttgggatca atattgggtt aaattagaag ctgcaacaac gggaggtaat atgccagatg
     5521 tagtaaccat gcactctagc gaatcctata aatatatgtc acagaatgct ttgatgggac
     5581 ttgatgatgt tatcaaagaa aatgatattg acatgggaaa ctttactgaa ggaattgctg
     5641 atttctatac gcttgaagac gaactatacg caatccctaa agatgcttct gtagtggggc
     5701 tatggtacaa caaagaatta tttgataatg caggtgtcga ttatccagat gaaacatgga
     5761 catgggaaac actcagacaa gctgcaattg atttaacaga tgaatcaaaa ggcatctatg
     5821 gttttgcatc acatattgga acagagactg gctactggcc atttatttat caaaatgggg
     5881 gagaagtatt tgccgatgac aatacaaaat caggtttgaa taccccagaa gcacaagaag
     5941 ctttagcttt ttatcgagat attgcacttg ttgatcatgc ttcaccaaca attacagaat
     6001 tacaagatcc tgataagtta agtcgttttc aatcaggtag attagcaatg atgattgaag
     6061 gaaactggtt tgcaccggga tttttgaaaa atgactatct agttgaaaat gttgcttctg
     6121 ccgtgttacc taagggaaaa gttaatgcaa caattacaaa tggacttggt tgggctgcca
     6181 gtgcgagtac aaaataccca gaagaagtga aaaaactaat ggcattttta gctagtgagg
     6241 acgccaataa aatccaagcg gaaacggggg catcaatccc agcactaaaa ggctttgaag
     6301 acgagtgggc tgcttcttca gatacaattg atttgagtgc ttttggaaaa atgttggagt
     6361 atggagtccc tcgtccatac aataaagatg gattacgagc tgaacaagca gaagttcaaa
     6421 ttattaaccg tattctgtct ggtgaagtaa ctgttgaaga tggaaccgct caaattgaaa
     6481 aagaagtcaa tgcgatattg agcggaaatt aaattagtta attatacaat atacaggcga
     6541 aaggattcta tttcgtctgt atattaatgg atgaatacca aaaaggggga tattatgaaa
     6601 aatcgaattt ttggaaaata tcgtaaagat caagtgatat ggggctatat ttttattttt
     6661 ccaacggcat taggattaat tttattaaat atatggccag caattcaaac agcaatttta
     6721 agttttcaaa aaacggtagg gttcggtgat actgaatggg tcggattaag aaattaccaa
     6781 aaaatgtttg cagacggtga agtttttcag tctttattaa acacattaat ttatgcagtg
     6841 gtttcgatac ctagtattgt tgtattatcc ttgttggtgg cagttttaat gaatagaaaa
     6901 gttaaaggat tgtcgattta tcggacaatt tattttctac cagtcgttgc tgcaccatcc
     6961 gcagtcgcaa tgatttggcg ctggatgttc aataatgatt atggactgat taataacatg
     7021 ttggctaaaa taggtctcga tggaccagca tggttaacgg atccatcgat tgcgatttat
     7081 tctattatca ttgtaggagt ttggagtgct attggttata acatggtgtt attactagcc
     7141 ggcttataag aaattcctaa agattattat gaagcagcgg aaattgatgg tgctagtcca
     7201 atccagcagt ttttcaacat tacgttacca ttagttacac caacattatt ttttgtagtt
     7261 gtaacaacag ttatcaatgc tttccaagta tttgatgtta tttttatgat gattactcct
     7321 aatagtaccg ccatgtttaa atcacaatca ttagcctatt tattctataa acattcattt
     7381 attttaaatg acaagggata tggttcggca atcgttatgt tccttttagt gattatatta
     7441 attattactg caattcaaat gaagttgcag aaaaagtggg taaactatta aggaggctat
     7501 caaattatga aaaataaatt tttttcaaat gcgccaatcc atatcatgct tattttaggt
     7561 ggtattgcaa tggcattacc ttttatttgg atgattttaa cttcagggaa aacgttggtt
     7621 gaatctaccc aaattccacc tacaattttc cctgagaaat ttcagctaag taactatcaa
     7681 caagtacttg atttattacc attcatggct ttttatatca atacattttt aatggttgtt
     7741 ggacgagtga ttggctcggt tttattttca gcaatggctg catatgcttg tgcgcgtcta
     7801 agttttccag ggaaaaatat ccttttcagc ttagtactta ttcaaatgat gattcccggt
     7861 tccattttta ttactccaca gtatttatta gcacaaaaat tgggtatttt aaatacaatt
     7921 ccagcattaa tattaccagg ggtagttagt gcgtttggta cgtttttatt aagacaattt
     7981 ttcttaggtt tacctgatga attggaggaa gcggcctatt tagaaggtgc caatatttgg
     8041 acgatctttt tcaaaattat gttgccgctt gctaaatcag gattggttgc acttggaata
     8101 ttcacagcgt tgtttgcatt taaagattta atgtggccat tgattgtcaa tacttcaatt
     8161 gataagatgc ctttagctcc gggattagca aacttacaag gacaatatgc cacgaacttc
     8221 ccacaattaa tggcaggttc gttattagca atttggccaa tagttgtgat attcatcgtt
     8281 ttccaaagaa aattcattga aggaattgct acaacaggta gtaaattata aaactattaa
     8341 ttagtgattg gagagtaagt acatgacaaa aattacattt attggtgcag gaagtactat
     8401 ttttgcaaaa aacgtattag gcgatgcgat gttaactcca agtctacaag atgcacatat
     8461 tgcattgtac gatattgatg aaaaacgact aaaagaatca aatttaatgc ttaaaaatat
     8521 taataaaaat tcgaatgaaa atcgtgctga tattgtttct tattatgatc gcaaagaagc
     8581 gttaagagag gctgattttg ttatcaatgc tattcaagtg ggtggttata aaccttcaac
     8641 agtgattgat tttgatattc ctaaaaaata tgggttgcga caaactattg gtgacaccat
     8701 ggggattggt gggattttta gaaccctacg gacactacca gtaatgttag actttgctaa
     8761 agaaatggaa gaagtttgtc caaatgcttg gttattgaac tatacaaatc cgatggcgat
     8821 gttaaccatg ggtgttttaa aagcgacgag tattaagact gttggtttat gtcatagcgt
     8881 tcaagtatgc gtaccagaat tgtttgagca tttaggaata gccgatcaat atgacttaaa
     8941 tgactttcaa tggaaaattg caggcattaa tcatatggca ttcttgttgg aaattacaaa
     9001 aaatggggaa gatttctatc caacaattcg tcagttagct gcagaaaaac caaatccaca
     9061 tccagattct gtacgttttg aattgttgaa acgttttggt tactatatta cagaatcaag
     9121 tgaacacaat gcagaatacc atccatactt tattaaatca aaatacccag aattgattga
     9181 cgaattaggg attccgttgg atgaatactt acgtcgttgt gaacaacaaa ttaatgattg
     9241 ggaaagcatg cgagaggaca ttgtaaataa tggggagtta acacatacga gaagccgtga
     9301 atatgcttca tatatcatgg attctattac gacaggaact ccgtcagtga ttgcaggaaa
     9361 tgtcttaaat aaaggcttaa tcacaaactt accgacagat gtttgtgtgg aagtaccttg
     9421 tttagtcgat aaaaatggcg tgcaaccaac ctatgtcggt gaattgccaa cacaattagc
     9481 tgctttaaat cggacgaata tcaatgttca agaactgaca gtagaagccg ctttaacact
     9541 agataaatca aaaatttatc aagcagcgta tttagatcca catctatcat ctgaattgtc
     9601 tttaacagat atcaaaaatt tagtggatga tttaatcgaa gcacatgggg actatttacc
     9661 tgaatataat taaaaagaag ctgaaagagt aaggaatttt caattcctta ctcttttctt
     9721 agttaaattt agtaaaaata ttgacaaaac acttagtaaa cagttacatt agtgttaaca
     9781 aaaagaaaac gcattcaaat tgtataagga ggagcataaa atgaagtggt ctaaaaaaat
     9841 tattttagct tgtatagcac taggagcaac gatctctttg actgcttgtg gaaaaaatca
     9901 ggaagatgca agcggtaagg tagaaataga gttttttaat caaaaaaaag aaatgactca
     9961 aacgattcag gagattgcta aagattttga agcaaaaaac cctgatattc atgtaaaagt
    10021 tgtggatgta ccgaatgctg gtgaagtaat taaaacacgt atgcttgcag gcgatgtacc
    10081 agatgtcatt aatctttatc ctcaaagtat agaattgaaa gaatgggcga aagctggtta
    10141 tttagaggac ttaaccgaag aaccgtattt agagaatatc aaaaatggat atgcacaacg
    10201 ttttgctatt gaggatcgtg tatatagcat accattaacc gctaatgtct atggttttta
    10261 ctataacaaa actgcttttg aggaaatggg gattcaagca ccagaaactt gggcagaatt
    10321 tgaaaaaata gtagctgaga ttaaagatca aaacaaagta ccgtttgcga ttgcaggagc
    10381 agaaggctgg actttaaatg gttatcacca actagctata gcgactgctg ctggtggtga
    10441 agaggaagcg aacaatgttt ggcgtttctc agaagttaat ggaattaatg ctgaaagcaa
    10501 agaaatgcaa aaagatttta atcgattaga tttgttaaga gaaccaaaag ctttgcaaaa
    10561 caactggcaa ggtgccggtt ataacgatac agtagttact tttacaaaag gtgatgcgtt
    10621 gattatgccg aatggttcat gggcaatgcc gatgattcat tcacaaaatc cagattttga
    10681 agtaggaact tttccttttc cagcagatga agcaggtcaa agtctaacga ttggcgcagg
    10741 agacctagcc ttatcagttt ctgcaacttc tcaacataaa gaagaagcca aacgttttgt
    10801 agaatatatg acaacacctg aagcgatgca gaaatattat gatgtagatg gtgcaccgtg
    10861 tgctgttgag ggcgttatcg agaatactca ggattcacca ttaagtggtt taactgaact
    10921 tgcttttact gatcgtcatt tagtttggtt agccaaagat tggaatagtg aaaatgattt
    10981 ttatacctta acaacaaatt atttacacaa tggcaatcaa aaagccatga ttaacgcatt
    11041 aaatgctttc tttaatccaa tgaaagctga cggaaggtag gaaaataaaa tgaaaataaa
    11101 agaaatttta aatcgttact gggcaatcat ttttgtagca attccaatta ttttgcagat
    11161 gatttttttc tactttccaa tggttcaagg tgctttttac agtttaacta attggactgg
    11221 tctgacttat aattttgatt ttgtgggttt gaacaattat aagattcttt tttctgatcc
    11281 gaagtttatc aaatcgattg gcttcacttt aattgtgacg ctttgcatga ttgctggaca
    11341 aattggtttt ggtattttga ttgcgcgggc gttgaacgca aatattaaag ggaaaacatt
    11401 ctttagagca tggttctttt ttccagcggt cttatcgggc gtggttgttt ctttagtatt
    11461 taaacaaatt tttaactatg gtttaccaaa aattggtagt atgttaggca ttgaattttt
    11521 acaaaccagt cttttaggaa caacatgggg agcgattttt gcagtaattt ttgttttact
    11581 ttggcaagga attgccatgc cagtcatcat tttcttagcg ggttttcaaa gtattccaca
    11641 agaaattatt gaagctgcta gaatggatgg ggcgaatgat aagcaaattt tttggaatat
    11701 tgaaatcacc tacttattac caagtatttc aatggtattt attatggcat taaaaggtgg
    11761 cttaacagca tttgatcaaa tttttgcttt aactggtggt ggaccaagcg atgccacaac
    11821 atctttgggg ttacttgttt ataattatgc ttttaaaaac aatcagtttg gttatgcgaa
    11881 tgctattgct ttagtcttat ttatcttaat tgggattgtt tcactggcac aattaaaatt
    11941 atcgaaaaaa tttgaaatct aggaggtttc ccaatgaaaa catcaaaaga aaaaaatatt
    12001 tctaaatact tgcttttatt attagggggc ttcatgattt tagttccctt agtttcaacg
    12061 ctattcggtt catttaaaac aacacccgat attatgaata atttctttcg ttttcctaat
    12121 ccagcgactt tggaaaattt ccgaagattg ttagcggatg gaattggtca ttacttttgg
    12181 aattcggcaa tcatcacaat cattgcagta gttgctgtga cagcaattat tcctatggct
    12241 gcttattcac tagcaagaaa aatgactcgt gggaaagcat acggattgat gtatttattt
    12301 ttaattttag gaatttttgt tcctttccaa gtaattatga ttccgattac cgttatgatg
    12361 agtaaaattg gtttagcaaa tatgtgggga ttaatcttac tctatctcac ttatgccgta
    12421 ccacagacac tatttttata tgttggtttt attaaactaa atgtgcctat cagtctggat
    12481 gaggctgctg aaattgatgg ggctagtcac tttacaactt atttcaaaat tgttttccca
    12541 atgttaaaac caatgcatgc gacgactatt attcttaatg ctctatggtt ttggaatgat
    12601 ttcatgctac cattattaat tttaaatcgt gattctaata tgtggacact accattgttt
    12661 caatataatt atacgggaca atattttaac gactatggtc caagttttgc ttcctataca
    12721 gtaggaattg tcacgattac actagtttat cttgctttcc aaagaagtat tatagctgga
    12781 atgagcaatg gagcagtgaa ataaccaata aattattctc attataaagg agttcataaa
    12841 cttatgatta tatttgacaa tcacacacaa acatttcatt tacaaaataa aaaaattagc
    12901 tatctattag gaatcgaaga aaataattat ttaacgcatc tttattttgg aaaaaagatt
    12961 acgcactatt ctggtgggta tcgctacccc cgaacagatc gttccttctc acccaacaat
    13021 gcaaatgcgc aagatcgttt attatcttta gacacattac cattagaatt tccaggatat
    13081 ggacatggtg attttagaga accggcgcat aatattaaat taggaaacgg tagtcgtgta
    13141 aatgattttc gttatgatag ctacgaaatt caaaaaggaa agcccagctt agcgggattg
    13201 ccagccttat atacggaaga tgatgatgaa gctgaaacgc tgattattca attgaaagac
    13261 gcgattagtg gattgacttt acagttgttc tacacaattt atcaaaatca cagtgtaatt
    13321 attcgttcta cgcaattagt aaataatagt gatgaaacag tggagattaa ccgcttagct
    13381 agtcaatcaa ttgattttcc aaatcgtcca ctcgagttaa ttcatttaaa tggtacctgg
    13441 gcaagagaac gtcaaatgac tagagaaaaa attcatacag ggataaaagt attagatagt
    13501 aaacgcggat caagcagcca tcaacaaaat ccgtttgttg ctttagtaga tgccaaaaca
    13561 gatgaatttc aaggagaggc atatggattt aatttagttt atagcggtaa tcacgaaacg
    13621 gtgattcaaa aagatccatt tgaccaaaca cgggtcatta tgggaattaa ttcatttaat
    13681 tttggttggc aactaaaaac tggcgactca tttcaaacgc cagaagtcgt gatggtttat
    13741 agtaatgaag gtctgaatac gttatcacag acgtatcatg aattgtataa tcaccgttta
    13801 attcgtggaa actatcgttt aaaagaacgt cccacgttaa tcaacaactg ggaagcaacg
    13861 tattttgatt ttaacgaaga aaaaatcatg aatattgtcg atgatgcgaa gaacttgggc
    13921 attgaaatgt ttgttttaga tgatggttgg tttggcggtc gtgaaaatga cttcacttca
    13981 ttaggtgatt gggttgaaac agaaggcaaa cttgcttgtg gtttggaaaa tttagccaag
    14041 caagtgcatg ataacgggat gaaatttggg atttggtttg aaccggaaat gatttctgaa
    14101 gatagtgaac tatttcgagc acatccagat tgggcattgg gtattccagg tcgtgaccgc
    14161 tcaattagtc gaaatcaata tgttcttgat ttttcaagac aagatgttcg tgataatatc
    14221 tatcaacaaa tgacggcgat ttttgatcgt gtaccgattg attatgtgaa gtgggatatg
    14281 aatcgaaata tgacagaagt ctattctgtc ttgttggact caacgatgca aggtgaagtg
    14341 gctcatcgtt atatgttagg gttatatgaa tttttagaaa agttaacaac gaattatcca
    14401 actattttat ttgaaagttg ttcgggtgga ggcggtcgtt ttgatgcagg tttcttgtat
    14461 tacatgccac aaacttggac gagcgacaat accgatgccg tggctcgtct gaaaattcaa
    14521 tatggaacca gcttagtgta tccaatttca agtatgggtg cgcatgtttc agcagtccca
    14581 aatcatcaaa caggaagaga aacgagttta actattcgtg gcaatgctgc gatggctggt
    14641 gtttttggtt atgagctaga tctaacgcaa ttaacttctg aagaaaaagc agaaattaaa
    14701 gaacaagttg ccttttataa aaagcatcgc caacttttgc aatatggaac tttttatcgc
    14761 ttggaaagtc cgtttgaagg caatgatact gcatggatgt ttgtctcaaa agaccaaaca
    14821 gaagccatgg tcttttattt ccgagtatta gcagaagctg cgcatccatt agtcacattg
    14881 aaattagcag gattagcaga agaagcaact tatcaaatgg aagatgtaac atttgctggc
    14941 gatgaattga tgaatctcgg tttttatatt gactcagaac tacatggtga ttatgcgaca
    15001 caacgctttt atttgaaaca gatatagtag cttgaattta caatataagt gcaacaccaa
    15061 aagagactca ttccaaacga agaaaatatg ttttatttac acgcatttac taaaaagcag
    15121 cctgatttga attgggaaaa tgaagaagta cgtgaagaaa tctatgcaat gattaactat
    15181 tggttagata aaggactagg cggatttaga atcgatgcga tattaaacat caaaaaaaaa
    15241 ctagagtatg gcatctttga atctgatgga gaagatgggt tagccttcat tgggccctgg
    15301 attcttaatc agtcgggcat tgaagtgtgg ttaaaagaaa tgagagagcg gacatttaaa
    15361 ccacataata gtatgacggt agcagaagcc gatgttccta atgaacgttt agatgaatat
    15421 attggtgaag atggctatta ttcgatggtt tttgatttta gttatacgga tattgatgta
    15481 cctgaaactg gtgaatggtt taaagatagc caatggacat ggacggatat gcgtaccaat
    15541 atttttacga atcaattagt tacgcaagat aaaggttggg gcgccttata tttagaaaac
    15601 cacgatcaac cacgttcaat taataaatat attccagaag aatggatcaa tgattatagt
    15661 aaaaaaatgt tggcgacgct ttttatgtta ctaagaggta ccccttttat ttatcaagga
    15721 caagaactag gaatgaccaa catagaaatg gcatcattag aagattttga tgatgtagct
    15781 acgcattctc agtataaacg ggcattagaa tatggattgg caccagagca agcattgaaa
    15841 gctgtcaata aacgaagtcg ggataattct cgcacaccta tgcaatggaa tacgcaaaaa
    15901 aatgccagat tctcaacttc tgataatgta tggttaaaag taaatcctaa ttatccagaa
    15961 ctcaatgcag aagctcagtt aaccaatcaa cagtcggtat tcaattatta tcatcaatta
    16021 attaaattaa gaaaaaatag tgtatacaaa gacgtgctaa tctacggaca atttctacca
    16081 gtgaaagata aaaatgaaca tattttgctt tatgaacgcc aattagaaga taaaatcgta
    16141 ttagttatct taaacgttac agcaacagaa caagaatatt atgtagatga acagtaccaa
    16201 caagtattaa tcaataatta tgaagatgtt ttattagatc aaagtcgaaa attgcgttta
    16261 cgaccgtttg aaagtattgt attagctaat tatggaggac aattatgaaa attaaaaacg
    16321 aagcaatgct catcacctat gcagatagtt taggaaaaaa catgggcgaa ttaaaacaag
    16381 tcttagacaa acatttacaa ggtgttattg gtggtgtaca tctgttgcct ttttttccat
    16441 cgacaggtga tcgaggcttt gcgccgagtg attatacgac agttgatcca gctcttggaa
    16501 catgggaaga tgtggaagca ttaggtgaag attattattt gatgtttgat tttatgatta
    16561 atcacatttc tcgtgaatca aaatttttcc aagatttcaa aaaaaaccat gagcagtcac
    16621 catacaaaga aatgtttatc cgtattcatg aatttttccc agaaaatcga ccaacacaag
    16681 aagatattga tttaatctac aagcgtaaag acaaagcgcc gtttcaaacg gtagaatttg
    16741 cggatggtac gacagaagag gtttggaata cctttgggga agaacaaatt gatttagatg
    16801 tgaccaaaga agttgtgaaa gaatttattc gagaaaccat caaagatatg gcgaaccatg
    16861 gctgttcttt aattcgatta gatgcgtttg cttatgcgat taaaaaatta gataccaatg
    16921 atttcttcgt ggaaccagat atttgggaac ttttagacga agtgcgtcaa gaagcagcga
    16981 aatatgacgt cgaattatta ccagaaatcc atgagcatta ttccattcaa atgaaaattg
    17041 ccaatcatga ttattatatc tatgacttcg ccttgccaat ggtcactctg tattcacttt
    17101 atagtggaaa atcagaacgt ttagccaatt ggttgaaaat gagtccgatg aagcagttta
    17161 caacattgga tacgcatgat ggaattggtg ttgtggacgc tcgtgattta ttaacagatg
    17221 aagaattgga ttacacctct gaggaattgt ataaagtcgg ggcgaatgtg aagaaggtct
    17281 attcaagtgc caattacaat aatttagata tttatcaaat taacagcacc tattatagtg
    17341 cattaggcga taatgaccgt agctatctgt tagcacgtgc gattcaagta tttgcgccag
    17401 ggattccaca aatttattac gttgggttat tagcaggaaa aaatgatatt gaactgttgg
    17461 aagcaacgaa agaagggcgt aatattaatc gtcattacta tgatttagcc gaaattgaag
    17521 aagaagtgca acggccagtg attcaacaat tatttgcact tttaaaattt agaaatgaat
    17581 ctgcggcgtt tgatttggat ggaacgattg aagtagcaac gccatcagaa tcagaaatag
    17641 tgattacacg taaaaataaa aacggtagtg atagtgcaac attaacggct aacttggata
    17701 cgaaagaatt tatcattgag tctaaagata taaaaatttt cactagtgtt gcattaaaat
    17761 tttaacctat tccatagaac aaaaaatcct ttatactagc acttggatga cttatccaag
    17821 accaataaaa aggactcata catggataag tataaaacaa atacagcatt taataaatgg
    17881 ttttctgcca taaaattagg aaaactttct gtcaactccc aaaaatgtat ttttgattac
    17941 aattcgtatc gtaaaaaact gtcttttgaa gctgtgctca agctctttct ctacgcagtt
    18001 aatgaagaaa aagaaagttt gcgtgatttg agtacagctc ttatcgaccg atcgctccaa
    18061 ctagaaaccg atgtgtcatc gattagtcat acccaattgt ctcgagcatt taatgcactt
    18121 gaacctaaag tgttagaaga aatttttcaa caactgttag aaaaagttca acatcaaatc
    18181 aaaccaacca agcgaaatca tctgtatctg attgattcca gtacgttttc gcttagttta
    18241 aaacgtcata agtgggcgac tttccgtcaa acaaaatcag gtgtcaagtt acacttaaac
    18301 tattgttata tggacgatac tacgatgtat ccaactgatt ttaccttaac aaatgcccat
    18361 gaacatgatc tcaatcaact accagtattg gtgaatcaac cagaggcaac ttacgtgttt
    18421 gatcgtgggt attaggactt tgaaaagatg gatcagatgc attgggatgg ctatttcttc
    18481 gtcactcgga ttaaaaaaaa caccaaagtt cacgtgatag atactctgga aacatcgcca
    18541 gaaacagaaa ttctacgaga cgagctggtt cgtttaggat ctaaaaccta tctaaccgcc
    18601 aatttccgac tagtcactgt tcaagataag aacggacgtg tgtttcaatt tatcaccaat
    18661 cgaatggacg tctcttcaaa agaaatctct gatatgtatc acgctcgttg gcaaatcgaa
    18721 ttgtttttca agcacatcaa acaacacatg accattaaaa ccttcttttc tcaaagtgaa
    18781 aaaggcgtgc agaatcaatt gattttaacg atgatttccg ctctcctcac gtttctcatc
    18841 aaattggaga cgaaaacgga aaaatcagtg ttccaaatca aacgattctt tcgttattta
    18901 ctgtttcagc cctttgaatg ttgtctagaa aaattaattc ccacatgaga aaaaagaaat
    18961 ttggctcgtt acttttggtt ttcagttgaa taacagataa aaaattttct ggaaaaagtc
    19021 atcctctgcg tcagcacagt tttttttgac ttattttcag aggaaatagt tttcagaata
    19081 ttctaaaatg ttttatgcaa cactagtgat attttttaga attagatttc taatgtgcat
    19141 tttttgagta ttaaggagca agtatacaaa gatttataaa gctgattaaa tttgaataaa
    19201 aatatgcagg aacgctacta tttttagtag tgttcttttt tatatttgaa taaaaaatgt
    19261 ggtggttaaa tggtggttat aacaaaaaat gaattaatga aattaggtta tgggaagtat
    19321 cagtctgaga gaatcatacg acaggcaaaa gcaattatgg tttcaaaaag ctatacctat
    19381 tacgaaaata gaagattagg actagtacct gttgaatctg tagaagaagt tttaggtatg
    19441 aagatagagg tagaggagga ttttcagaat catggttaat tttaaaaaag ataaaaatgg
    19501 gaaatatcat tttactgcta gtttgggatt cgacaaacga acagggaaaa gagtacaaaa
    19561 aaacgtagag gggttaaaac agttaaagaa gaaaaaaagc gtataccgaa cttgtttcta
    19621 attatggaaa agaagcagaa acttctcaaa gtactatgtt gtttgaggag tttttttata
    19681 aattattcct accttattat caagggaaag tagaagaaag aacatataaa aatcggttat
    19741 ctacaatcaa aaaaactttt cttatttctt taaaatggag ttaagagaaa tacgtccaat
    19801 tgacattcaa aagtggcaaa ataatttatt gagcaaatat aaaaattctt atgtaagaaa
    19861 catgtatggg atttttcaga tgtcattaga tcgagcagtt gttttgggac tacttgaaaa
    19921 aaatcctgca aaagttattg gaaatgtgaa gaaaaggaaa actacagttg atttttggac
    19981 aaaagaagaa tttacaaaag ttatttcaac tttttatatt gatgatttgt accaacattt
    20041 tggttttatt tgtatgtggt tactttttat gaccgttatg cgtttgggag aagccactgc
    20101 attaacttgg aaagatattg attttgaaaa taaaaacttg cgtgtgaata aaacactcta
    20161 ttataagaga gccaaaacct atgagtttgt agaacccgaa acggcttcta gtattcgtac
    20221 aatcgaacta gacgacaata caattacctt tttaaaagct tggaaggagc gtcaagtttc
    20281 aataggaggc atgtcgtttg tactttctta taatggaaca cctactcaga aatatacaat
    20341 ccgtcatatt atgagaagac atgcaacgat cgttggtgtt catataatcc gcatacacgg
    20401 attaaggcat agtcatgctt ctttgcttat atcacaataa aaagatgcac ttcgaattaa
    20461 agaaaggtta aaacatgctg atgtacaaac gactttagga acttatggtc atttgtatct
    20521 aaattcattg agagagataa caaatgatcc cacagattat atccaaacta atttttctaa
    20581 aatcaaaccg acacctattt acgataataa aaaataaatg ataaacaaaa gtaggatcac
    20641 accaaaagaa acacactaga aaaactataa tacaggcttt tctagcgtgt tgtttcttat
    20701 ttccgcctat ttatacatta tactcataaa gcatttaatg catcgatcgt agccgcattg
    20761 tctatatggg tatataaagt tgttgtattg gtacttgaat gtcccaattg ttgggcagtt
    20821 aataccaatg aatttgtctg ttggtagagt ctagtagcta atgtatgacg cagtttgtga
    20881 gggctgactt gaattctaaa gccttttgaa tattttgcta ccatccgttc gattgcaata
    20941 ccggatatac gcttggcttc tccttggtaa attgctagaa ataaagcttt ttcgttttct
    21001 gttactttat atcgttcttt ccgaatagct agataattag ctaaatattc cattgcgaat
    21061 gctgctatat tgacagcatc tcttttattt ccttttctag ttactaccac attattctta
    21121 tctaaagata ggtcatctaa attaatattt ataacctcgg agagtctcaa tccagatcct
    21181 aaaatcagtg cattgatcgc caggtctctt tctacatctc gttgataata gatcaaagct
    21241 cgtttactca aagtattttt atattcattg gcaatataat ctagataatt ttgtgtatcc
    21301 ttatctagaa ataatttttt ctcaatggca ttggcacgtg cagctaacgt agttttatct
    21361 gtgatggttg ctacttgatc cataggatta ttgtctaagt atggttttcc tagaggatta
    21421 ttggaacttt tcgaaagata tttaaataga acctttaatg ccgtgatatt tctattgata
    21481 gtagaagtag atagacctaa gttattatta tctttgagct tagtttttgt tgtctctttt
    21541 tttacatttt cttttgtgcg acgtttcaaa tgagatttgt acaagtttag gtcataggca
    21601 gttaactgtc ttatcgaggt gatagaagta tcaggaacga tagactcact cagaagccat
    21661 tcataaaaga gcttataatt ttttagatat tctaaagtgg ttcgaggtga ttgatcttta
    21721 tcctcacgat cttgaacaaa atcttttata taatcgggca tatgaagtat gattgtatct
    21781 attttttgat atatttctgt atttttcatg tcattcatcc tttcaataag taggggagaa
    21841 gagtatctct ataattggac tataccaatt atagaatgtt ttgtcaattg gtatgctcta
    21901 aaatttatta att
//
LOCUS       DACDIG010000050        20510 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.050, whole genome shotgun sequence.
ACCESSION   DACDIG010000050 DACDIG010000000
VERSION     DACDIG010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.050"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(170..3319)
                     /locus_tag="IV247_001897"
     CDS             complement(170..3319)
                     /locus_tag="IV247_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP8777962.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEE
                     QIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDNLIHA
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3333..4550)
                     /locus_tag="IV247_001898"
     CDS             complement(3333..4550)
                     /locus_tag="IV247_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP8777963.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(4540..6135)
                     /locus_tag="IV247_001899"
     CDS             complement(4540..6135)
                     /locus_tag="IV247_001899"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP8777964.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYSISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
     gene            6260..7045
                     /locus_tag="IV247_001900"
     CDS             6260..7045
                     /locus_tag="IV247_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HAP8777965.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
     gene            7754..8113
                     /locus_tag="IV247_001901"
                     /pseudo
     CDS             7754..8113
                     /locus_tag="IV247_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(8408..9541)
                     /locus_tag="IV247_001902"
     CDS             complement(8408..9541)
                     /locus_tag="IV247_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8777966.1"
                     /translation="MLLNFKMTLTMLAFSLLVTGVILVSFFFLRNNLEGISIASTLGE
                     DVYFMEEGKTVQAETQMEASKSSNNQFMTISMIDKAYQDELFKYLPAMVLVICSAVML
                     LTFILWLVLRKIYSNQILAITRDLTFIDQVPKNTVKDEALNAAFNKLKDKFDGHLEDY
                     RRLNSYLTHEQKNAIAILRTNLEIERQHADQGNLMLLDRMTDSIDDILTLSNNSEDLS
                     TEVVDVSLICAEVCDAYKMNYPNLLFAFTEDQPTLIHGRDRWIYRAVSNLVDNAIKYG
                     EGKLVIVTISNQKGSVIIEVKDQGIGIPQEKQASIFSHQYRVHGLKQDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            complement(9532..10200)
                     /locus_tag="IV247_001903"
     CDS             complement(9532..10200)
                     /locus_tag="IV247_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8777967.1"
                     /translation="MRVLVVEDNRELVLSVKKGLEQENISVDVAYDGESGEEKAFINS
                     YDGILLDLNLPKKDGLSVLQSLRAEEVESPVIIITARDEIEERAKGLDFGADDYLVKP
                     FELLELKARIRAVIRRYHGRALPQISIGALKIDPLGRRVSYQKTEVVLKPKEFDILLC
                     IAQRHPAVVSSEEISEHVYDEDFDPFSSVLRVHLARLKKKLAEAAEKEVLKTIRAKGY
                     QLCY"
     gene            complement(10656..11642)
                     /gene="lacD"
                     /locus_tag="IV247_001904"
     CDS             complement(10656..11642)
                     /gene="lacD"
                     /locus_tag="IV247_001904"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP8777968.1"
                     /translation="MNEEKRQYIKQLCNEDGVIGALAIDQRGALKKMITKFKGSEAEE
                     SEIVEFKEIVSSQLTKYASSILLDPEYGLPAADVRAKNAGLLLAYEKTGYDASTPGRL
                     PDLLSIWSVKRLKEAGADACKFLLYYDVDEAEEINNQKHAFIERIGSECQAENLPFFL
                     ELVSYDANISDATSKEYAIKKPHKVNDMMKEFSDPRYGVDVLKVEVPVNMNYVEGYGT
                     DEVVYTKAQAANFFKEQAEATNLPFIFLSAGVKAELFQETLRFAKNAGSTFNGVLCGR
                     ATWADGVESYIVDGKEAAMEWMNTQGRKNIEELNAVLKETATPVEVLTSSLS"
     gene            complement(11655..12581)
                     /gene="lacC"
                     /locus_tag="IV247_001905"
     CDS             complement(11655..12581)
                     /gene="lacC"
                     /locus_tag="IV247_001905"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HAP8777969.1"
                     /translation="MILTITMNPSIDISYPLETFKLDTVNRVRNVRKTAGGKGLNVTR
                     ILKQLDTEVIASGLIGGFLGEEIKKDLTQNEIAHDFLPISGETRNCIAVLHDGKQTEI
                     LENGPVITEQEARAFLQHFEKLAVKAEIISFSGSLPEGLPADFYRQMLQVCQPKPVVL
                     DCSGTALREVLRGSVKPKLIKPNTEELAGLLGREISTELSDLKAALSDSMFDEIEWIV
                     VSMGGDGAFAKHHETFYCVTIPKIDVVNPVGSGDATVAGLTAAIQQGLSDEAVLKQGN
                     VLGMLNAQEATTGYVQLANYQKLYDQILVEKV"
     gene            complement(12594..13109)
                     /gene="lacB"
                     /locus_tag="IV247_001906"
     CDS             complement(12594..13109)
                     /gene="lacB"
                     /locus_tag="IV247_001906"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HAP8777970.1"
                     /translation="MKIAIGCDHIVTDTKMAVSDFLKSKGHEVLDMGTYDFTRTHYPI
                     YGKKVGEAVTSGEADLGVCLCGTGVGINNAVNKVPGIRSALVRDMTTAIYAKEQLNAN
                     VIGFGARITGEFLICDILEAFINADYQETEENKKLIAKIDHVEKASDKQSDPHFFDEF
                     IEKWDRGEYHD"
     gene            complement(13128..13556)
                     /gene="lacA"
                     /locus_tag="IV247_001907"
     CDS             complement(13128..13556)
                     /gene="lacA"
                     /locus_tag="IV247_001907"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HAP8777971.1"
                     /translation="MRVVIGSDSDGMELKELIKKKLIKEGITVVDKSETASEDFVEST
                     LAIANDLNENEGSLGIAVDGYGAGSFITATKVKGMVAAEVSDERSAYMTRKHNNSRMI
                     TLGSKIVGDELALNIVKEFLQANYDGGRHQVRVDMLNKMA"
     gene            complement(13576..14349)
                     /locus_tag="IV247_001908"
     CDS             complement(13576..14349)
                     /locus_tag="IV247_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8777972.1"
                     /translation="MLKTERQRSILAMCDRYGTITVKFIQEVLNVSDMTIRRDLEELA
                     QKKKLLRVHGGAQKIDYLEMSTSIEANELSHTEKKKLHTKEKEYIAKNAAAHINQEDD
                     TIFLGSGTTIELMTRYIKPSSLRIVTNSLPVFNLLSEIKRFELYLIGGLYREKTGAFV
                     GSIANEAVQKIGIKKAFVGVNGLKGSEVSNFSIEEGTLQKIVLDNAAEKCLVSDANKF
                     DHSDFYNFYDLRKIDKLFTDSTLSAELKSYYQQFTKIIQ"
     gene            14514..14993
                     /locus_tag="IV247_001909"
     CDS             14514..14993
                     /locus_tag="IV247_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8777973.1"
                     /translation="MNEFFFKDTVYVSDKQSQEEVFEEVYQDLLKKELVTEDFLENLL
                     ERERNYPTGLDLSPVSKILPDIAIPHTESEFVRTTRIIPIKVKNEIPFYNMISPSDQI
                     SASFLFMILNENGEAQAGMLANIMDFINSVDEEKLFSFFKLEDTEAIYHFLEEHFYI"
     gene            15050..15352
                     /locus_tag="IV247_001910"
     CDS             15050..15352
                     /locus_tag="IV247_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003088862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAP8777974.1"
                     /translation="MIKILAACGAGVNSSHQIKSALETELKNRGYQVTCDAVMIKDIN
                     EEMLSHYDIFAQIANTDLGFDINIPVVDAGAILYRIPAMAEPVYQSMESAIQSLNK"
     gene            15374..16831
                     /locus_tag="IV247_001911"
     CDS             15374..16831
                     /locus_tag="IV247_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol transporter subunit IIA"
                     /protein_id="HAP8777975.1"
                     /translation="MNTIIDIANKVFTPLINLGAAPLMTIVLTVIALFFKVKPSRALE
                     GGLKLGIALTGIGAIIGILTTAFSDAMTAFVERTGISLNITDVGWAPLATITWGSPYT
                     LYFLLIMVIVNIVMLAMKKTNTLDVDIFDIWHLAIVGLFAIFCGANLFIASALVIFIG
                     VLKIWNSDLMKPTFNDLLNAPDSNPMTTTHMNYMMNPIIMVFDKIFDKIFPWLDKYDF
                     DAAKLNSKIGFWGSKFAIGIYLGIFVGLLAGQTPTQIFSLAFTAAVCLELFSLIGAWF
                     IAAVEPLSQGITDFANKRLKGRTINIGLDWPFIAGRAEIWAAANVLAPIMLLEAIILP
                     GNKLLPLGGIIAMGVTPALLVVTRGKLIRMIVIGAIELPLFLWSGTLIAPFVTQTAKA
                     VGAFPAGLNGSAMISHTTMEGPIEKFLGYLVGNASQGQIEFVIYAALALGAYLLIFIW
                     YARQMKKRNAAYAAEKEENASPATAHNMTYAAEAK"
     gene            complement(17424..18176)
                     /locus_tag="IV247_001912"
     CDS             complement(17424..18176)
                     /locus_tag="IV247_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8777976.1"
                     /translation="MLKTDRFQAIMSLLEVNGTVRVSDIMEKFSVSDMTVRRDLDEME
                     KQGLLERVHGGAKLKDFYRHVELSHREKQIMLQEEKQRIVEKANEMINDGDTIFLGPG
                     TTIELMSKIIQGDSLRIVTNCLPVFEELNKRKGNRKIYLLGGEMRETTQCFVGEITNK
                     SLQDMNFHKAFFSCNALNEQKIMTSTFEEGQTQQIALNNSVERFLLIDHSKIGKRDFS
                     VYYNLNDVTVVITDDDEQKHYEKIEKEEKYVV"
     gene            complement(18391..18627)
                     /locus_tag="IV247_001913"
                     /pseudo
     CDS             complement(18391..18627)
                     /locus_tag="IV247_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
     gene            complement(18637..19601)
                     /locus_tag="IV247_001914"
                     /pseudo
     CDS             complement(18637..19601)
                     /locus_tag="IV247_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(19807..>20510)
                     /locus_tag="IV247_001915"
     CDS             complement(19807..>20510)
                     /locus_tag="IV247_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922316.1"
                     /note="phosphoenolpyruvate-dependent sugar
                     phosphotransferase system; catalyzes the phosphorylation
                     of incoming sugar substrates concomitant with their
                     translocation across the cell membrane; IIB is
                     phosphorylated by IIA and then transfers the phosphoryl
                     group to the sugar; IIC forms the translocation channel;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HAP8777977.1"
                     /translation="LLFATPIILNPYFLIPFLLAPIANVWIFKLFVDVLGMNSFISVL
                     PWATPAPIGLVLGTGFSVLAIVLAVVLIVVDAIIYLPFVKAYDSELVEQELKNIAAEE
                     VKEELVEEIQEVGATAAVIVDEAKASVGEGKRVLVLCAGAGTSAMLANALEEGAKETG
                     VNLSAKAGAYGSHYDMMKNFDLIVLAPQVNSYLDDLRKDADKLGIKVIATKGAEYINL
                     TRDPKGAVDFVLKQF"
BASE COUNT         5952 a         4312 c         3469 g         6777 t
ORIGIN      
        1 tgcaacagaa ccggtaaatg aatgaaaaag atggaagaat cattattcaa tagctttctt
       61 aaatactccg ttataaaatg tgaacaagta aaaagacatc tatccctatt ttctacactt
      121 ccactttact actatctttg aatcgtacaa ttttcttaac tcttctcgtt tacctcaatt
      181 cattttcaag tggaatcaac tcatcatcta acattttacg ccatgctcgc ttcatttctt
      241 gtgggtattt aaatggtttc gcttctggat gtttggcttt atatccttcg taatccttac
      301 tgttgataat tgctttcata ttaggaattg gatcttcacc tggcatatat tgcagattag
      361 aagcatgtaa ttctttctca gaaacaaacc actcattgga aaaggcatga atcaaattat
      421 cacttgcttg tgtaaaatag cgacgtttaa tgacaaagat atcttcatta ctatctaaag
      481 tccccacatc aaatgcattt aaaatttgtc ggtaaacttc tttgactcct tctgttttat
      541 tcaatttagc caaagctttt tcaatttctt ctcgtgcatc tgaactattc gcagcataag
      601 ttcctagtaa ttgatcaata taagtagaat caatatcata caacttagtg gtattttcac
      661 tataaaattc gatcgttgaa aaatcttctc cctctttttc tggttcttcc tccatcaatg
      721 aacccttcac ggtctcgtac acgccaattt gttcttccaa aagttgaatt tgattgttca
      781 aagttgaaga ttgcaccatg tcatcattgt aatcatcata agtcactaac gattcatacg
      841 cacgattcaa ttcttgaaat gctttgacgt actcgatacg gatttcaagt ggcgtatgtt
      901 catcaatgga ttccatggat gaaacctctt ttaacttacc atatgcttgc ttgaatcttt
      961 ttttggattc ttggtaactt ggataaatca aacccgattc ttctttagct tctgaataca
     1021 gttttgtcgc atcctcgaca ttcttctcca ttgtcgctgg ctttcggaaa gtaacaatta
     1081 gtccttttgt tttttcagga tacgtacggt tcgtacgtga aaaggcttgg atcaatcctg
     1141 catactctag attacgatcc acaaataggg tttgaatcgt aggggcgtca aaacctgtca
     1201 atagacgatc cactacgatt actaaatcga tttgtttccc aaattgtttg aattccgctc
     1261 tttttcgtgc caaacgatta ttgatgtctc cgttataacg ctcaatgtct gctagtgacc
     1321 aggcagtatc ataatactga ttatattcct caataatttt ttgcatttct tcttgttggg
     1381 cacttgaatc tcggctattt tcttctaatg aataggtaat agcgattcgt gggaaatcag
     1441 gatcctccat cgttcgtcct tcacggatag gctgattggg aaactctttt attaaccaat
     1501 cagattcttt cgtcatctct ttgatggccc gataatactt tttggccata tcaatggaac
     1561 tcgtggttaa gatcgcagac ttcgttggac ggccatttcg aaaatcaaat tttgtatagg
     1621 cattgtctgg acggaacatt ttatgaatga ctttttggat atgttcctct gcttcaaaaa
     1681 cagtagggtc caaataactt tcttttttga cagactccat ttgatcgatc atacgattga
     1741 tctgttccgg tgaatactct gcatatgttt ctacttcttt taatttccga tagattttat
     1801 tctctaatga agtcggctcc acggtgtttt catgctctac ttgaaatcct aaaaccgagc
     1861 catcttctaa tgcattcttg atggtatagg tgtgcaagac ctctccatac tgatcatggg
     1921 tcgtacgtgc caattgccct ttggcttgtt tccgattctc agggaaaatc ggtgtacctg
     1981 taaagccaaa ccaagtggat ttcggaaaca tttttttgat ttcttccata ttttcagcac
     2041 ttaacgctcg atgacactca tcaactacaa acacgatatg ttgccccatt aatttttgga
     2101 agcgattggt tccttttttc tcttcctgtt tctttgcata tttcaacgcc gcatctagtt
     2161 tttgacgggt tgtaatgatc acaacattcg cattggcatc tgccaacaga gtttcactta
     2221 actctttggc acttcctgtt cccacgatca agctattcgc tttggcatta ccagaagaaa
     2281 ccccagtatt aaactccgaa gcaaatttcg taaactctgt ggtggtctga ttatctaaat
     2341 ctttgcggtc cactaacatg atcgttcgat cgatccccga ttttcgtgct aaaagcttag
     2401 tagatacaaa actagtcaac gtctttcctg aaccagtcgc atgccacaca taccctgatt
     2461 gatgtttatt tgcggcaata aacaaggctt gaatcgcatg cacttgataa ggatgtaaca
     2521 ccatgagggt tttattgtct tgatcttcac tgacaatcgt ataatccgca atcaagcgat
     2581 gggcatctgg gatatttagc acttgtttgc aaaactcata taagttttct acctttttat
     2641 tatctttcgt tcgccaacta aataaaaact taggatgcaa atccttcggt aaggcatttg
     2701 caaaataccg agtcgtttgt tcattggaaa ccacaaacaa ctgtaaggtc gaaaagatat
     2761 tgttccgaaa catgccttct tcagcatatt ttttgatttg attaaacgcc tgataaaaac
     2821 catcttttgc agttacttgt tttagctcga tttggataat cggtaagcca ttgatcaata
     2881 aggtgacgtc aaaacgtcga tcccgcgctt ctatgttact tccacgtttt gcaatttgat
     2941 ggaccacttc ataagtagag attcctccac caatgtcttg attggaataa aggataagtg
     3001 agacagaacc caattgcgga ttttcccgtt caatcgtgat ccgagccatc ccattttcac
     3061 cttttaacca tttcgcagca tcaaatgggg ttttcgtccg tagcaataat tcggttttga
     3121 tcgtttcaaa ctctttatcg gtcaaaggag aatcattcag ttctgcttga ttatttgaga
     3181 tgattttttg acgtaaattg ctccaaagat cttcttctga ttttagatcc ggacgatacg
     3241 tccactgatt atgtccttcc ccgaggacac gaatcaactg ttcttcgact tccgcttcct
     3301 cacggtgact aattttcatt cttcttccct ccctaaacaa acatcttttg caagaagcct
     3361 tttttcgttt ccttcagcaa atctaactta cgttgatgaa gagcgatcgt atcatctagc
     3421 tgcttgaaga aggcaccaat tttttgttgt tcatttatat ccggtggaaa ctgaaatgtc
     3481 ctttgttcta taccaccttt agtcacatgt accattgtcg ttccgttagt tgattcttta
     3541 attttctctt tatcagtcaa tagccaagta tatagatact gtttgtccaa ttccttagat
     3601 aaaaatttaa gtttccaaat atgataatgg tagattacac gttcttcatt ccacatttca
     3661 ggaccgaagc ttgttgccca taaatataag agatctcctt tattagcata cttgtcttcc
     3721 tctaattcta agtttgaata ataccatcta tcattagtat taaaattacc tactcttaaa
     3781 accctatatt ttccagaatc aagtaattct tgttgtttaa aagctctacc attcaaaaaa
     3841 actaccactt cccccaactt ccgctcttcc caatctcccg taaatcctgg aaaacgaact
     3901 tctggcactt ttgctccgtt tttagggaac attttttgta agaatccttt tttcgtttcc
     3961 ttcagcaaat ctaattcgtg ctggtgaagc atgatggtgt gatcaagttg tttgaaaaat
     4021 gaaccgatct tgatctgttc gtcataagtt ggaagtgaga taggtgtctt tttaccacta
     4081 tcaattgtat aggttccgac ggttccaatg cctgcaacgt cacggatata cttctgaatt
     4141 ttgttattta tgaacgagta gtaaaagaag tttccgtcaa ttttatgctc attcttgaac
     4201 caaatgatgt ttccatcctt aaagtaaatt gggttatcat cagtgattaa taaggggaca
     4261 cctattgatc cgacaccagt tacaagcaag tctccttgag aaactttacc tgaatttttc
     4321 gaatattcat tatatttttc ttctgaaatg taaagataat cggatggttc ctcatttttt
     4381 gcagctgaga caatatcacg agcacgtaaa aatctaatac ctgagttagt ccaatctgat
     4441 tgatgaattc gcttcacaga agtaacatcc atcatatctc ctaacttacg ctcttcccaa
     4501 tcttccgtaa aaccaggaaa ccggatttct ggtcgtgtat cattgctcat gtttgaacac
     4561 ctccagcgca ccttgtaacc acgcttcatt ttcaggtgtt gtttgcaggg aagagatagc
     4621 ttctaaaagt tctttttcta gcacttgctg ttcttgtcgt atcttcttga tgtcatttcc
     4681 gacatgaacc atatcaattg gttcttcttc ttcgaaagta tcgacatagc gtgggatatt
     4741 taagttatag tcattttctt tgatttcatc aaaggttgcc aaatgagcgt atttctcgac
     4801 atcttttctt tcagcgtagg tttcgaggat tttttgaatg ttttcttccg ataatttatt
     4861 ctgatttttc cctttaacaa attcacgact agcatcaatg aataatacat ctcgagtttg
     4921 acgattcttc ttcaatacaa tcaccgtcgt tggaatcgat gtaccaaaga acaagttcgc
     4981 aggcataccg atcacagcat aaatactgcc gtcttctaat aatttttgac gaatgacccc
     5041 ttctgcagca ccacggaata aaaccccgtg tggcaagacg atagccatag tccctgtttc
     5101 cttcaaatga tagaaaccat gaagaagaaa cgcaaaatct gctttggatt ttggcgctaa
     5161 tttcccgtag cgattaaacc gagaatcatc taagaaggtc gtatctgctg accaattagc
     5221 cgaataaggt ggattcatca ctactgcatc aaatgtgtaa ggttcatctg ttggccagtc
     5281 cttattcaac gtatccccat tgcggagatt catttcttcg gcatctaccc catgtaaaat
     5341 caagttcatt tttgctagat tgtatgtcgt tgtatttagt tcttgcccat gatatttaac
     5401 gttatctgga tgagttaagt agttacgtac atttaacatc aaggaacctg aacccatggt
     5461 tgggtcaaag acactaaaga atcgtcgttc tttttgatcc aacgtcacga tttgtgccat
     5521 catatcagaa accatatgag gcgtgtaaaa ttctccggct ttcttccctg cttctgaagc
     5581 aaattgactg atcagaaatt catacgcatc cccgatcaca tccccatcgt gacctaaaac
     5641 ttccacgtca ttcaatttct tgataacttc ggtaatcgtc acatttcgtt gttgctcatc
     5701 tgttcctagt ttttttgatt ggagatccac atcatcaaat aacccattaa attgattgta
     5761 ggtagatgcc aattgcacaa aggctttgtt tagatcgttc aattggaacg ttgcttgttt
     5821 tgcttgatcc gctaagacgt tgaaaagata ctctggtgag atcgaatacc ccaaaatatc
     5881 aacgattgtg gcaatcaggt cttcttttga ttcttcatct gaaagtaatt cacgatacaa
     5941 cattgtttgt ttgcttactg tgtcatattc ctctaaggat tcatctgcta aaagaaccac
     6001 ttgttccaac aaacggtcgg ataaatattt atagaaaatc agtcccaata aataattttt
     6061 atattctgac gcatccattt tactacggag attatctgcc gcactaaata atttttgatt
     6121 taattctgca cccaaaaaga tacactccta tctcttattt aaagttgcta tataccctct
     6181 tattttagca tactcccttt tagaaaaagc aaaaaaaaaa gaaaaaatct tattcttgat
     6241 aacaagctac gtgacgtata tgcctaacaa aaatagaact tttgaatcaa tcttaaatga
     6301 aggtttaacc acagaaccgg catccattat ttttgatgaa gttatcgatg acgttgaaga
     6361 tagtaagtat cataaaaatt taaaagaact tttttatttt gcactcaaca atggaccaag
     6421 tttgttcact ggtatatcag tacctgatag tgataatcca ttaattatgg caaaaattta
     6481 cttaacaaaa tggtgtaata aatacatcag ggataggaat aacccagcat taaaaaaacc
     6541 tcttaaaaca tttggcgaaa aagatgctgc tttaactact agagtagcag caaatgctaa
     6601 tattgatgat caaaaagtat tgaatgacta cttgcgtgga cattttttat atatgagcgc
     6661 tgagaacatg aacggaagta ttttagagga atatttagcc gaagtgttag aacctgaagg
     6721 ttggatttgg tgcgccggct ctgtatatcg agctgttgat ttctgttatt taggaacctc
     6781 accaattctt ttacaagtga aaaataaata caatacagaa agcagttcga gttcagctat
     6841 aagagtagga acaacaatta gaaaatggaa tcgtttaaat aagtcgacaa aaatttctgg
     6901 tttggatagc cctatcccca attggaaagc attaatagaa atgactgaag ctagtaagga
     6961 actggcagct aagttaactg aaaatagtta tttagcttat attaatgaaa aatccacacg
     7021 agaattatgg acactcgatg actaacaata tccttattag tatctatcca cgatacatgg
     7081 aaaaaattat aaacaaacaa aaaaactttg aatttagaaa tttctatctt acctcagatc
     7141 cttataatat cttttggatt tacgaaacaa tccccaccaa agctttaaag tataaattga
     7201 ttgtaaaaaa tccaattact aaacttaaga aaaatcaaca ctacttttta ggagacgatg
     7261 agtttgaaca attagtttaa aagggaaaat atgcttctat cattacaagc tcgtttggtg
     7321 taagatgggt ataagtcatt tatgttcact ctccttgtat gctttagcgg gtattacaat
     7381 ttgagtgtaa cataaatggc ttttttattt gtctcgctta attatacaaa cggcgaatta
     7441 taaataactc ataaaaaata ttttgacttt caaaatcact tttgctacca gaagttgagg
     7501 cgaagtgttt taagcatagt tgtaccctag tttgaccgtt aaaaataaat aacgatcgct
     7561 agagtgcaac tatgtttgtt tgccctaccg tcagcgtcgg cttggcctct cacatccatt
     7621 accgtcggga ttatctccgt tacataccct tacaaaatac gccctgtaag cattgcatga
     7681 aaccaccgtg catttgcacc gtatttccgc cctagccgtt caaatcggtc gctaccacca
     7741 ttcagaaaca cgtgcggtaa tggaaggttt ggaggaagct atccagacca ctaacgattg
     7801 tccctatcgt cgtacgtttc actcagatca aggttggtcg tatcaaatga aagcttatag
     7861 aaacaaattg aaggagtaca aaatctttca aagcatgtct cggaaaggga actgcttaga
     7921 taattcactt atggagaatt tttttggctt attaaaacaa gagatttttc acgggaaagt
     7981 ttataactgt ttcgaggaat taaaatcagc catcgatagc tatatctatt actacaataa
     8041 tgaacggata aaacagaaat taaactggca aagtcccgtc caatttcgta aaacaaccgt
     8101 tacggttgtg taaaaatatc attttagagc atgaaaaaaa gagtggattt ctccactcca
     8161 aaaaagtcta acttttgggg gtcactacat ttttgatcct ttttatttga ttaattatat
     8221 ccagctggat aataattttg ggggcaacat ttctatctta ctattatctt cctaaagtag
     8281 tagactttct tattgggaaa aattacaaca aaaaggagtg gcttagttga caactccttt
     8341 ttgtttacaa atcaattgga tatctataaa ttgtattttc taacgaattt tatagacatc
     8401 tgatttgtca aattaagcta gacaatatgt ctttatctat gcagctcaaa aatacttcca
     8461 atggtgttcg ataatttaat gattttcttg gaatatggtt tcttttcgag gcaacggatt
     8521 gaataaaggt ttcatccgtg tccctaaagt ccattgtttt aggtaagcca tcctgcttga
     8581 gcccatgcac tcggtactga tgagaaaaaa ttgatgcctg tttctcctga ggaatgccaa
     8641 ttccctgatc ttttacttca ataatcacgc tacccttttg gtttgaaatg gtgacaatta
     8701 ccagttttcc ttcaccatat ttgattgcat tgtcaactaa attcgaaacc gcccgataaa
     8761 tccaacgatc tcgtccatga atcagggttg gttgatcttc agtaaacgca aacaaaagat
     8821 tcggataatt cattttgtag gcatcacaaa cttccgcaca gatcagtgaa acgtctacga
     8881 cttccgtgct aagatcctct gagttattgg ataatgtcaa aatatcgtca atactgtcgg
     8941 tcatccgatc taaaagcatt aaatttcctt gatcggcatg ttgccgttct atttccagat
     9001 tcgtacgcaa aatagcgatg gcatttttct gctcgtgggt cagataacta tttaatcgtc
     9061 gataatcctc caaatgacca tcgaacttat cctttagctt attaaaagcc gcatttaatg
     9121 cctcgtcctt cacagtattt ttaggaactt gatcaataaa cgttaaatca cgcgtaatcg
     9181 ctaagatttg attactgtaa attttgcgta aaaccaacca aagaataaac gttaataaca
     9241 tgacggcaga acaaataacc agcaccatcg ctggtaaata tttaaatagt tcgtcctggt
     9301 aagctttatc tatcatcgaa atagtcataa attgattgtt ggaagatttc gaagcttcca
     9361 tctgcgtctc tgcttgaacc gtcttaccct cttccataaa ataaacatct tcacctaagg
     9421 tagatgcgat actgattccc tccaaattat ttctcagaaa gaagaaactc actaaaatca
     9481 cgcctgtcac caacagtgaa aaagccagca tcgttaacgt cattttaaaa tttaatagca
     9541 tagctggtac ccctttgctc gaatggtttt taagacctct ttttcagctg cttcagccag
     9601 tttctttttt agacgagcca gatgcactcg cagaaccgac gaaaaaggat caaaatcttc
     9661 atcgtagaca tgttcagaaa tttcctcaga cgaaacaacc gctggatgac gctgagcgat
     9721 gcacagtaag atatcaaatt ccttgggttt caggaccacc tctgtctttt gatagctgac
     9781 ccttcgacct aatggatcaa tcttcaaagc accaattgaa atctgcggca gcgcacgtcc
     9841 atgataacga cgaataaccg cgcgtattcg tgcttttaat tctagtaact caaagggttt
     9901 gactaaataa tcatctgctc caaaatccaa gccttttgct cgttcttcaa tctcatcacg
     9961 tgcagtaata atgataactg gactttcgac ttcttcagca cggagacttt gtaaaacaga
    10021 taatccatct tttttaggca gatttaaatc tagtagtatc ccatcgtatg aattgatgaa
    10081 ggccttctct tctcctgact cgccatcata agctacatct acagaaatat tctcctgctc
    10141 taggcccttt tttacggata aaaccagctc acgattgtcc tcaaccacca gtacacgcat
    10201 gtatattcac tcctcatacg gatttagtaa ttacacttat gctttttagc atagtacacg
    10261 ctcttcttca aagtccacgt tctttttgtg aagatttgaa gaaattcata cattctgtta
    10321 aaaaacacat aattaagaat gattttactg tttgaaaata cctagaatag aaaaagactg
    10381 gaacctttcc tccagtctca ctactttcta tttgcttggt tttcttgtat atacttttca
    10441 aagttcgttg gtttatgacc aataagcctc gttagaatac tgaaaattgc catcaagtca
    10501 ttaagtacca catgtaagaa aatctctcta aagaaaaacc ccttcgtttt aactcactta
    10561 ctattttaga attcgttccc ctaataatgc agcatgattt tacgctttat gtataaaaaa
    10621 agactgcaag caattttgct tcacagtctg atttcttatg atagtgagct tgttaacact
    10681 tccaccggtg tagcagtttc tttcaatacg gcatttaatt cttcaatatt tttacgccct
    10741 tgcgtattca tccactccat cgctgcttct tttccatcaa cgatatagct ttctacacca
    10801 tctgcccaag tcgcacggcc gcaaagtaca ccattgaagg ttgagccggc atttttcgcg
    10861 aagcgcagtg tttcttggaa caactctgct tttacaccgg cgcttaggaa aataaatggc
    10921 aggttagtgg cttcagcctg ttccttgaag aagttagccg cttgtgcctt tgtgtacacg
    10981 acttcgtctg tcccatagcc ttccacataa ttcatattga cgggtacttc cacctttaac
    11041 acatctacgc cataacgtgg atcagaaaat tccttcatca tatcgttgac tttatgaggt
    11101 ttcttaatgg cgtattcttt cgaagtcgca tcagagatgt tcgcgtcata agaaacaagc
    11161 tccaagaaga agggtaagtt ttcagcctga cattctgagc cgattcgttc aataaaggca
    11221 tgtttttgat tattgatttc ttctgcttca tcgacatcat agtaaagtag gaacttacaa
    11281 gcatcggcgc ctgcttcttt taaacgttta actgaccaga tcgataataa atctggtaaa
    11341 cggccgggag ttgacgcatc gtaaccagtt ttttcataag ctaacagcaa gccggcattt
    11401 tttgctctaa cgtcagctgc tggcaagcca tattcgggat ctaataaaat cgatgaggca
    11461 tatttcgtta attgtgaaga aacaatttct ttaaattcaa cgatttctga ttcttctgct
    11521 tcgctgccct tgaattttgt gatcatcttc ttcaaagccc cgcgttgatc aattgctaag
    11581 gccccaatca ctccatcttc attgcatagt tgcttgatat attgacgttt ttcttcgttc
    11641 atttccgtat cctcctacac tttctccact aagatttgat catatagttt ttgataattg
    11701 gctaattgga catagccagt cgtcgcttcc tgagcattca gcattcctaa aacattaccc
    11761 tgttttaaca cggcttcatc cgaaagacct tgttgaatcg cagcggtcaa tccggcaacg
    11821 gtcgcatcac cagagccaac tggattgacg acatcaatct ttggaattgt cacgcaataa
    11881 aaagtctcgt gatgtttggc aaaggctccg tcgcccccca ttgaaaccac tatccattca
    11941 atttcatcaa acatcgaatc tgacaaggcg gcttttaaat ctgataattc tgttgagatt
    12001 tctcgtccta gcagaccagc cagctcttcg gtattgggtt ttattagctt aggctttaca
    12061 ctgcctcgca acacttccct taatgctgta cctgaacaat ctaaaacgac aggctttggc
    12121 tgacaaactt gcagcatttg tcgataaaaa tcagcaggca atccttctgg caagcttcct
    12181 gaaaaagaaa tgatttcggc tttaacagct aatttttcga aatgttgcaa gaacgccctt
    12241 gcttcttgtt cagtaataac aggtccgttt tctaggattt ccgtctgttt tccgtcatgt
    12301 aaaacagcga tgcaattacg tgtctctccg ctgataggta aaaagtcatg agcaatctca
    12361 ttttgagtca ggtccttttt aatctcttca cctaaaaaac cgccgattaa accactagca
    12421 atcacctccg tatctaattg cttcaagata cgtgtcacat ttaggccctt accgccagct
    12481 gttttgcgaa catttcttac tcgattgact gtgtcgagct tgaaggtttc taatggataa
    12541 gaaatatcaa tcgatggatt catcgttatt gttaaaatca tttgtccacc cgcttaatcg
    12601 tggtattcgc cacgatccca tttctcgata aattcatcga agaaatgcgg atcagactgc
    12661 ttgtcactgg ctttttcgac gtgatcgatt ttggcgatca attttttgtt ttcttcagtt
    12721 tcttgataat cggcattgat gaaggcctct aatatgtcac agatcaaaaa ttctcccgta
    12781 atccgagcgc caaagccgat cacattggca tttagttgtt ccttcgcata gatagcagtc
    12841 gtcatatcgc gaaccagcgc actgcggata ccaggtactt tattgacagc gttgttaatc
    12901 ccaacacctg ttccacacaa acaaactcct agatctgctt ctccgctagt cacggcctct
    12961 ccgacttttt ttccgtagat cggataatgg gttctggtaa agtcataggt ccccatatcc
    13021 aacacttcat gtccttttga ttttaagaaa tctgatactg ccatttttgt atctgtgacg
    13081 atgtggtcac agccaattgc gattttcatt tcattcatcc ttcctaatta cgccatttta
    13141 tttaacatgt cgacacgtac ttgatgacga ccaccatcat aatttgcttg tagaaattcc
    13201 ttaacaatat tcaacgccaa ctcatcgcca acaatttttg atccaagtgt gatcatccga
    13261 gaattattat gtttacgggt catatacgca ctgcgctcat ccgaaacttc tgctgctacc
    13321 attcctttaa cctttgttgc cgtgataaag ctgccagcgc cgtagccatc aaccgcgatt
    13381 cctaaactac cttcgttttc attcagatca tttgcgatcg ccaaagttga ttccacaaaa
    13441 tcttctgaag ccgtttcact cttatctact actgtaatac cttctttgat caattttttc
    13501 ttgattaatt cctttaattc catgccgtca ctgtctgaac caattactac acgcattttt
    13561 gtttcctcct attttttact gaatgatttt agtgaattgc tgataataac tcttcagctc
    13621 ggctgaaaga gtagaatccg taaataattt gtcgatcttg cgcaaatcat agaaattata
    13681 aaaatcgcta tggtcgaatt tgtttgcatc tgaaaccaaa catttttctg ccgcattatc
    13741 taaaacaatt ttttgcaagg taccttcttc aatactgaaa ttcgaaacct ccgaaccctt
    13801 caatccattc actccgacaa aagccttctt aataccgatt ttttgaacag cttcattggc
    13861 aattgaaccg acaaaggctc cagtcttttc ccgatatagc ccaccgatca aatacagttc
    13921 aaatcttttt atttcactta atagattgaa aaccggcaga ctattggtaa cgattcggag
    13981 agatgacggc ttgatgtatc gtgtcattaa ttcgatcgtt gtacccgatc caagaaaaat
    14041 cgtgtcgtct tcttgattga tatgagctgc cgcattttta gcgatgtatt ccttctcttt
    14101 tgtatgcagc ttcttttttt cagtatgaga aagctcattt gcttctatag aagtcgacat
    14161 ttccagataa tcgatttttt gtgcaccgcc atgaacgcgt aaaagctttt tcttctgcgc
    14221 gagttcctct agatctcggc ggatcgtcat atctgaaaca ttcagtactt cctgaataaa
    14281 tttgactgtt atcgtcccat agcgatcaca catcgctaag atcgaacgct gtcgttctgt
    14341 tttcagcata ttctgtacct ccacgacctt ataataaaac atttacgaac atttttcaac
    14401 acatattgtt cgtttatttt cgtttatgcg ttgacaatga ttttgtaagc ggttatactc
    14461 aacttgtaaa catataaata aacataaacg aactaataaa ggagctgttt tgtgtgaatg
    14521 aattcttttt caaagacact gtctatgttt ctgacaagca gtcgcaggaa gaagtgttcg
    14581 aggaagtcta tcaagatctg ttaaagaaag aattggtaac cgaagatttt ttggagaacc
    14641 ttttagaacg ggagcgtaat tatccgacgg gattagatct ctcacctgtc tctaaaatat
    14701 tgccagatat cgctatccct cataccgaaa gtgagtttgt gcgaaccacc cgtatcattc
    14761 caatcaaagt aaaaaatgaa atccctttct acaacatgat ctcgccaagc gatcagattt
    14821 ccgcttcatt tcttttcatg atcttgaacg aaaatggtga agcacaggct ggcatgttgg
    14881 ctaatatcat ggatttcatt aattctgttg acgaagaaaa actgttttcg tttttcaaat
    14941 tagaggacac agaagcgatt tatcattttt tagaagaaca tttttatata tagaagtctc
    15001 tgcctaaccg caggataaaa aataaactta ttttaagggg agaattatta tgatcaaaat
    15061 tttagcagca tgtggagcag gcgttaattc aagccatcaa atcaaaagcg ctttggaaac
    15121 tgaattgaaa aatcgtggct atcaagtcac ctgtgacgcc gttatgatca aagatatcaa
    15181 tgaagaaatg ctgtctcatt atgacatttt cgcacaaatt gccaacaccg acttgggttt
    15241 tgacattaac atccctgtcg ttgatgcagg tgctattctt tatcgtattc ctgctatggc
    15301 tgagcctgtt taccaaagta tggaaagcgc aatccaatca ttaaacaaat aggactaaag
    15361 aaaggaaaag catatgaata caattattga tattgccaat aaagtattta cccccctaat
    15421 taatttaggt gcggcaccgt taatgaccat cgtattaact gttattgctt tattttttaa
    15481 agtaaaaccg tcacgcgctc ttgaaggcgg gctcaaatta gggatcgccc tgactgggat
    15541 cggagctatt attggaattt taactactgc tttttcagac gctatgacag cttttgttga
    15601 acgcacaggt atttcattaa acattacaga tgtcggttgg gccccactag caaccatcac
    15661 atgggggtcg ccatacacgc tttatttttt actaattatg gtgatcgtca atattgtcat
    15721 gttagcaatg aaaaaaacca acacattaga tgtggatatt ttcgatattt ggcatttagc
    15781 aattgttggt ttgttcgcga tcttctgcgg tgctaatctg tttattgctt ctgcattagt
    15841 tattttcatt ggtgtattaa aaatttggaa ctccgactta atgaaaccaa ccttcaatga
    15901 cttgttaaat gcgccagaca gcaacccgat gaccacgact catatgaact acatgatgaa
    15961 tccgattatt atggttttcg ataaaatttt cgacaaaatc ttcccttggt tggataaata
    16021 tgatttcgat gctgccaaat tgaatagtaa aattggcttc tggggatcaa aattcgcgat
    16081 tggaatttat ttggggattt ttgttggatt actagcagga caaacaccaa cgcaaatttt
    16141 ctcgctagcc tttaccgctg ccgtctgttt ggaattgttc tctttgatag gcgcttggtt
    16201 catcgcagcc gtagaaccgt tatcacaagg gattaccgat tttgccaata aacgactaaa
    16261 aggacgtacc atcaacatag gtttagactg gcctttcata gcaggccgcg ctgaaatctg
    16321 ggctgccgct aacgtattag caccaatcat gttgctagaa gccatcatct taccaggaaa
    16381 taaattattg ccactaggcg gaatcatcgc aatgggtgtt accccagcac tattggtcgt
    16441 aactcgtgga aaattaatcc gtatgatcgt aatcggcgcg atcgaattac cattattctt
    16501 atggtccggc accttgatcg ccccgttcgt tactcaaaca gctaaagcag tcggagcctt
    16561 tccagccggc ttgaacgggt ccgccatgat ctctcataca acgatggaag gcccgattga
    16621 aaaattccta ggttatttag tcggtaatgc ttctcaagga caaatcgaat ttgtgatata
    16681 cgctgcctta gcattaggtg catacctttt aatcttcatt tggtacgctc gtcaaatgaa
    16741 aaaacgcaac gctgcatacg cagctgaaaa agaagagaat gcatctccgg caacggcgca
    16801 taatatgact tatgcagccg aagctaaata agctaaaaac accccgaatg tctttagact
    16861 tcggggtatt attttaccta taaaaaataa atagatgtaa aatccatttc aaaaagcata
    16921 gcccatctct tgccacaccc aataaagaaa ttatcaacgt ttatgctttt tttaactcct
    16981 ttaagcttgc ctttgttggt attcctcaac gattcttttt gtgttcttgt ttgttttgtg
    17041 ctttttgact ttttgttgtt taattgcgaa ttccttgatt tcttttttcc tgaatcgctt
    17101 cgcatcccgc ggttatgcgt gcgatttcat cattgctttt tctgatctaa ctcgtttcat
    17161 gtcaaagagc ttcagctgac ttagtgtcca tggttgtttt gatatccctt ttcctcctta
    17221 ataaagcggt gattgatact gctatttttt ccttcacata aacataacat cccaaaaaaa
    17281 tcgactatct tttttcaaat ggtgtattca agtattcgga ggaattagta atgatgcacc
    17341 ctaaaaataa cataattcgc ttaaattcaa ggctaccaaa aaactaccaa gttctaatta
    17401 tctagagttt ggcagtttct catttacaca acatattttt cttctttttc gattttttca
    17461 tagtgctttt gttcatcatc atccgttata actactgtta catcattcag attataatat
    17521 actgaaaaat ctcttttccc aattttcgaa tgatcaatca ataaaaagcg ttcaacagaa
    17581 ttattcagag cgatctgttg agtttggccc tcttcaaaag tcgaagtcat aattttctgt
    17641 tcatttaaag cattacagct gaaaaatgct ttatgaaaat tcatatcctg tagcgattta
    17701 ttcgtgattt caccaacaaa gcattgagtc gtttcccgca tctcgccacc taatagatag
    17761 atttttcgat tcccttttcg cttatttaat tcttcaaaaa caggcaaaca gtttgtaaca
    17821 atccgcagtg aatccccttg aatgattttt gacatgagct cgattgtagt tcctggtcca
    17881 agaaatatcg tatcaccatc attgatcatt tcattcgcct tttccacaat ccgctgcttt
    17941 tcttcttgca gcataatctg tttttctcta tgagaaagct ccacatgccg ataaaaatcc
    18001 ttcaatttcg ctccgccatg gacgcgttct aataatcctt gtttctccat ttcgtccaaa
    18061 tcacgtcgga ctgtcatatc cgaaaccgaa aacttctcca taatatcgct gactcgtact
    18121 gtaccgttaa cctctaataa cgacatgatc gcttggaatc gatccgtttt taacatttca
    18181 tcgcctcttt tctttttaag actatcaaat tcttaagaaa agtgctagaa caagttttct
    18241 taagaagcct tctgcaggaa ggaaagcaaa atttttcaga atttgcaagc tgctattcta
    18301 aaaaataaaa ccgaacgagc atgactgtca tagtgacagc cttgttcatt cgacttttat
    18361 ctttaataaa tcattttatt taatctgaag ttattgtgca agttcaaaaa caaagtagtt
    18421 aatgtctcct cgatatttcg aaatcgaata ctcaacgggc agacgttcta gcgaaaaacc
    18481 ctgtgtatgg aaatagaaca ttggatctcc ttcttccaca tctaacaaat cgctgatcgt
    18541 atcatccgct ttgattactt ctagttttct ggtgaccgtt ttaattggat aacccatttc
    18601 ttcaaaaact tgatacattt tttcttttct gatttgtcaa attaagctag acaatatgtc
    18661 tttatctatg cagctcaaaa atacttccaa tggtgttcga taatttaatg attttcttgg
    18721 aatatggttt cttttcgagg caacggattg aataaaggtt tcatccatgt ccctaaagtc
    18781 cattgtttta ggtaagccat ctcgtctaag caagccgttt gagtgctcgt gctcatttag
    18841 ccctctttgc gaaggtgttc ctggatctgc gaaaaagatg gcaatatcgt tcgagttact
    18901 tatagatcgc cagttggaaa attcttttcc acaatcaaat gtcatggatt taaacaagtg
    18961 tttaggcatg gatttgaacc aatcatttaa cctgttttcg atatcttcgg ctcgtctgcc
    19021 tgtcggtttc aaagtaataa taaccttgga taaacactca accaatgtaa taacagcgct
    19081 cttgtgttta actcccacaa tggtgtcgcc ttcgagatga ccaaattctt tagtcaggtg
    19141 tggatagaga ctgttccgtt catgaatcgt tctgcgaaag gcctgttttc cacgcttttc
    19201 ttgatgatta ttcggtttac gtttcccctt cataggtaac gactgtgtgt caaaaaggcc
    19261 ttttttaaac atccgataga gtgtacgaac agaacaatca attggaaact cgccacggcc
    19321 gataatcaca tcgggtgacc acccttgagc taccttttct tgaacatatg ttgattgttc
    19381 atcagataaa acggttgaac gtcgtccaca acgagattta ttttgtttgt attgacggta
    19441 ataatctagc acttttttcc ttgtttgaga aacgaataaa cattataaat ggtttgtcta
    19501 gagcgattta aactttgtgc gacaagagcg acaggtttct cgatattgaa ataagactct
    19561 atgattacta gttcgtctgt tgtaagatgg gtgtaggtca tttgtactca ctccttatta
    19621 attctttggt cggaactatt ttgagtgtag cacaaatgat ctttttagtt gtctagctta
    19681 attttacaat cgacgcctca aaaagaatac ccgaaattta aacagcaaaa aaagatagcg
    19741 cttaccattg tgtttaagat aaaacgctag ctaagttggc tagcgcttta ttaatctaag
    19801 caagctctaa aattgcttca atacaaaatc aactgctcct ttaggatcac gtgttaaatt
    19861 gatatattct gcacctttag ttgcaattac ttttatccct aatttgtctg catctttacg
    19921 gagatcatcc aaataggaat tcacttgagg tgctagaacg attaaatcaa aatttttcat
    19981 catatcataa tgagaaccat aagctcctgc tttagcagaa agattaacac ctgtctcttt
    20041 tgcgccctct tctaatgcat tagccaacat tgcactcgtt ccggcaccag cacaaagtac
    20101 taaaacgcgc tttccttcac caaccgatgc ttttgcttca tcaactataa ctgccgcagt
    20161 tgcaccaact tcttggattt cttcgaccaa ttcttctttt acttcttctg cagcaatgtt
    20221 ttttaactct tgttctacta attcagaatc ataggcctta acaaaaggta aataaataat
    20281 cgcatcgaca acaatcaata ctaccgctaa tacaatcgcc aacacactaa acccggttcc
    20341 taaaaccaag ccaatcggtg cgggcgtcgc ccaaggcaat acggatataa agctattcat
    20401 ccccaagaca tcgacaaaaa gtttgaatat ccatacgtta gcgattggtg ctaataaaaa
    20461 tggaattaga aagtacggat tcaaaataat aggagtagca aatagtaacg
//
LOCUS       DACDIG010000051        18578 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.051, whole genome shotgun sequence.
ACCESSION   DACDIG010000051 DACDIG010000000
VERSION     DACDIG010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.051"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            6..305
                     /locus_tag="IV247_001916"
     CDS             6..305
                     /locus_tag="IV247_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8777978.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IV247_001917"
     CDS             378..1739
                     /locus_tag="IV247_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP8777979.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IV247_001918"
     CDS             1761..2021
                     /locus_tag="IV247_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777980.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IV247_001919"
     CDS             2035..2787
                     /locus_tag="IV247_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAP8777981.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IV247_001920"
     CDS             complement(2881..3486)
                     /locus_tag="IV247_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8777982.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IV247_001921"
     CDS             complement(3539..4513)
                     /locus_tag="IV247_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAP8777983.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="IV247_001922"
     CDS             complement(4891..5670)
                     /locus_tag="IV247_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8777984.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IV247_001923"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IV247_001923"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP8777985.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="IV247_001924"
     CDS             7161..8558
                     /locus_tag="IV247_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8777986.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="IV247_001925"
     CDS             8639..9079
                     /locus_tag="IV247_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP8777987.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="IV247_001926"
     CDS             9227..9553
                     /locus_tag="IV247_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8777988.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="IV247_001927"
     CDS             9554..10303
                     /locus_tag="IV247_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777989.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="IV247_001928"
     CDS             complement(10383..10838)
                     /locus_tag="IV247_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP8777990.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="IV247_001929"
     CDS             complement(11265..11540)
                     /locus_tag="IV247_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8777991.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="IV247_001930"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="IV247_001930"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAP8777992.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(12978..14429)
                     /gene="cls"
                     /locus_tag="IV247_001931"
     CDS             complement(12978..14429)
                     /gene="cls"
                     /locus_tag="IV247_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP8777993.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            14770..17436
                     /locus_tag="IV247_001932"
     CDS             14770..17436
                     /locus_tag="IV247_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP8777994.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            17800..18552
                     /locus_tag="IV247_001933"
     CDS             17800..18552
                     /locus_tag="IV247_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8777995.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
BASE COUNT         5970 a         3212 c         3832 g         5564 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    12781 aggtcgtgag cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacagcttct
    12841 catgtttgtt gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttgttc
    12901 ggaagaaggg actgtgacaa gatttttgtc acagtccctt taatcttagt atatttttct
    12961 ctacttgaat aaaaaaatta taggattgga gaaaataatc gagaaatttg ttgcttgaat
    13021 aatagccagt tagacatttc cttgactgtt tcagttgtca tggatgaaca atgtgtctca
    13081 tcttctttga actgagtagc aagttcttct aaaaactcag gatcgtaaat gacagcactt
    13141 gtttcaaaat tcaatcggta gctacgaata tcttgatttg ctgaacctac catgcagatt
    13201 tcatcatcca ttatcaatgt ttttgcatgg aggaagccgt tctcatagac aaggatttgt
    13261 acattttcct tgatcagctg ccgagcataa tattgtgttg ctcgataaat aaaaggatga
    13321 tccggtttgt tgggaatcat gattttcaca tctacaccag aggcagttgc gatttttaaa
    13381 gcagcaatga cactttcatc aggaacaagg taaggtgtct gtatccaaac acgtttttta
    13441 gcagaagtaa tcaatttgat aaatgaaagc ttgatttgtt ccctttcgtt attcggtccg
    13501 cttgaaacaa gctggatgga tgtatcagca agcttagatt catctcgatc tgctttttta
    13561 aagaaataat tcaaatgata gccgaccttt ttttcttcag gtaccgagac gttccagtct
    13621 gttaagaagc gaagctggag caaagaagaa gcggcaccga aaatccgtat atgcgtatcg
    13681 cgccaatagc cgaatttttt tgttgtattt acatattgat tggcaatatt gaaaccaccg
    13741 gtataactaa tctttccatc gatcacaacg atttttcggt gatcatggta attcaatcgg
    13801 aaacgaagta atgccctttg tgaggtaaca aatgtatgga catgtccacc atttttgatt
    13861 agacgattga aatcttttgc ttttgtgcca tgagagccaa atgcatcata taatattcga
    13921 acttccacgc cttctgcggc tttttcttct aaaacatgta agacttgctg gccgatatta
    13981 tctgtcacaa atgcgtaata ttcgatatgg atcgaatgtt tggctttttt tagatcttga
    14041 agcagtgaat ccaatttctc ttgtccgtct gtgagaagag tgacagaatt cattcttgtc
    14101 agcggcatac gatttaatga agagaagaag tcaacaaatt gctgtttgtc tttatcgccc
    14161 atgtcaggat cgtaatgttc aatggtatct cctctaaaag actgaaagtt ttctaattct
    14221 ttcaagtcac tttgtcggag atagaatttt ttcttatccg ttaatccacg tccgaaaaat
    14281 aaatataata caaagcccac cccaggaagg gcaaacaata ctaagagcca tgcccaaatg
    14341 gctgctacat ctcggggttt gatcaaaata gccaccaaag caataagtgc atttaataga
    14401 taaagggcgg ttataatact agataccaca aaaatgttcc tccttacata caataacatt
    14461 agtatagcat gatagaagaa agaagcctac caaggtgaat atacttttca ctcctttcat
    14521 gaaaatgtag gtaaatcggg ctaaagtagt tgctttttgt aggttaataa ggtaggatat
    14581 tagtacagtt gaatatctcc ttaggggagt aaccggcaac gtttgttgcg ataatatcaa
    14641 cagtaagtta accaaatggt tttctggtat tgtatgaaat ggtgagacct aagacaaatg
    14701 cgtatttgtc ttaggtctct tttttttcag acaggactag ggccaagatc aaaaaatggg
    14761 ggatgaaatt tggaaaacaa ggaacaagca caacaagcgg tctataaaga aacgatcgac
    14821 tcgttgatgc agcaaacgca gagctcaacc gatggattga gcaccacgca agcgaaggaa
    14881 aaattagaaa gagatggctt gaaccagcta aaagaaaagc cgaagaagcc aacttggaaa
    14941 ttgttcttag aaactttcaa agatgcaatg gttatcgtat tgctgattgt cgcactgatt
    15001 cagatggtca tgggtgacta catcgaattc ttagttattt ttgcagtatt gatgttgaat
    15061 tctataatca gtgttgtcca aacaaaaaaa gcagaaggtt ctttagaagc gttgaaaaac
    15121 ctttcagcgc ccgatgctcg agtgattcgt gatggtaagg aaaaaacgat tccggcaaac
    15181 gaacttgtgg taggagatat cgtgttgtta gacgcaggcg attatgtacc tgctgatgga
    15241 cgtttactag atgcgggatc attaaaagta gatgaaggta tgctgacagg agagtcgact
    15301 tctgcagaaa aagaagtaac ggatatcaca agtacggtac caatcggaga tcgtacaaat
    15361 atggtattca gtggaacgat cgtaacttac ggacgcggta aattcttagt gacttcaact
    15421 gggaataata cggagatcgg acaagttgct ggccttttgg aatcaacaac tgagcaagtc
    15481 actccgttac aacgaggtct ggataaattc agtaaaaaac taagtttggc tattttggca
    15541 ttgtctttat tgattttagg tatccaactg tttcgtattt atcttggaga aggtacagga
    15601 gatatgactg cagatatcgt cagcgcagtg atgtttgccg tagccgtagc cgtagctgct
    15661 attccagaag cattacagtc tatcgtaaca atcgtgttat ctttgggaac gaataaaatg
    15721 gcaaaacgtc atgccattat tcggaagctt ccagcagttg aaacacttgg ttcgacaagt
    15781 atcatctgta cagataagac tggtacattg acacaaaaca aaatgacagt tgttgactac
    15841 tttttaggta atggcgatac tggggatttc acaaatgacc cttcaaaatg gacagctgac
    15901 gaacgtcgct tgatcgaaat cagtgtttta gcgaatgatg catcgatcag tgaagacggt
    15961 actaaattgg gcgaccctac ggaagtagcg tttatcgact ttagtgaaaa attaaaccag
    16021 ccttatcagg aaatccgaaa caactaccca agacaagcag aacttccttt tgactcagat
    16081 cgaaaattaa tgtctactgg acacacaatt gataacaaaa attacttgtt tgttaaaggc
    16141 ggaccagata tcgtcttcca acgcagtaaa aaagtattgc taaatggtga agtcgttccg
    16201 atgacggatg aattgatgaa aaaattccaa gagcaaaatg aagctttttc aacacgcgca
    16261 ttacgtgtac tagcttttgc ttaccgtcca cttgataatt ttgatttatc atttgaagat
    16321 gaagacgatt tgatccttgt tggtttgctt gctatgatcg atcctccacg tgctgaagtg
    16381 actcaggcgg taacagaagc aagaagtgca ggaatcaaaa caatcatgat tactggtgac
    16441 cacaaaacaa cagctgttgc tatcgcaaaa caaatcggga tttttgaaga aggaaacctg
    16501 gctttgaccg ggacggaact agatgaattg acagaagatc aactaaacga agttttagaa
    16561 aaagtcactg tttatgcacg tgtttcacct gagaataaaa ttcgtatcgt tcgtgcttgg
    16621 caaaataaag gtcatatctc agctatgaca ggtgatggtg taaatgacgc accagcattg
    16681 aaacaagcag acatcggaat tgctatggga acgggtacag atgtggcaaa agatgcagca
    16741 gcgatggtat tgacggatga taactttgct tctattgtca gcgcagtaga agtaggacgt
    16801 aatgtgtttg ataatatcaa gaaagctatt tcttatctat ttgcaggaaa tttaggtgcg
    16861 atcattgcga tcatctttgc cttagttgct gactggtcca atccatttac agcattacaa
    16921 ctgttgttca tcaatttagt caatgattca ctgccagcaa ttgcattagg tacagaaaaa
    16981 gctgaacctg ctacgatgaa ccgcccgcca agagatccaa attcaggaat ctttacggac
    17041 aaaacattga tttctgtaac gtatcgtggt gtgctgattg ggattgctgt tatctttgct
    17101 caatggcttg gcatgcagca ttcacctgaa ttcggtgtag ccatgtcatt ttcaacattg
    17161 atttggtgtc ggacattgca aacattccca gcacgttcaa acacacaaac agctatccaa
    17221 gcagggttct tctctaacag aagtgtctta ctagctgtag ttgtatgtag cgcgctttac
    17281 tgcttaacaa tgatcccagg actgaaagaa gtcttctcta tcccatcagc atttggattg
    17341 acagaagtct ttacatgtat gggaatcgca cttgtgacaa ttgttttgat ggaaatcact
    17401 aaattgatca ttgtacgagt acaaggaaat aaataaaacg aatcaacgag gttgtgagcc
    17461 tgccgtttaa ttctgagtca caaggtgcaa tttcaacaaa cagcttctca tgtttgttga
    17521 atattgtgac ttgtgaccga agagttggca ggagaacacc gtttaaggaa gaagttgtaa
    17581 aatcagcatt ttgagctaat actgagggtc atctgattgg acactgttta ttcggaaaga
    17641 gggactttga cacgactttg gtcacagtcc ctttttttat ttatgataga cggaaagcca
    17701 atcattttat tattcatcaa aagagataga atagataaaa gaggagaatg atttattccg
    17761 aaaagtgaaa acatataaca aaaaattaga ggaaatcaaa tgaatgaaaa agaatgggat
    17821 gaatttgctg aagaatatta tgaaatccag caagaatcgc aagtaactat tgctgaagat
    17881 gtaaaaaatt atctgaaaac ggagaaagtc ctgcctgctg aaacagcagc agacgttgct
    17941 ggaggatctg gcagatttct ttccttagca aaagaaatta atcgctatga tttgatcgat
    18001 ttttcttcag gaatgctaga aaatgcaaaa aaagaagcag aaaaaaatga gttgccaaat
    18061 gtttccttta tcaaacagtc gttagaagaa tttatccaat caaatgagac atatgaaatg
    18121 gtttttacag cagcaaatcc agcattggta catcttattc aattagaaaa attgatgaaa
    18181 aagtcaagca aatggtgcgt gataatcaga gtagtggaat caatcgatga tgttttttat
    18241 cctatcgaaa agcaactagg aatgatagaa gaagatccta atacatcgcc agttgtaatg
    18301 gatcaatttg aaacatactt aattgatgaa ggattcaaag tgaagaaaaa agattttact
    18361 tattttacac aggaatcaat tactgaagaa ttgatgaagc tatactatga cgcgtacgca
    18421 aaagatcctg ttgtgaccag gtacattcgc aaactattca gtgaaaaacg agaaatcctt
    18481 agtacgacta agctgaccta tagactgctc ctttggtccg cagataaaaa aagaaccttt
    18541 cacgactcgt gaagggttct ttttatgatt ttattata
//
LOCUS       DACDIG010000052        18555 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.052, whole genome shotgun sequence.
ACCESSION   DACDIG010000052 DACDIG010000000
VERSION     DACDIG010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.052"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            72..506
                     /locus_tag="IV247_001934"
     CDS             72..506
                     /locus_tag="IV247_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8777996.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(584..1117)
                     /locus_tag="IV247_001935"
     CDS             complement(584..1117)
                     /locus_tag="IV247_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAP8777997.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(1211..2389)
                     /gene="mutY"
                     /locus_tag="IV247_001936"
     CDS             complement(1211..2389)
                     /gene="mutY"
                     /locus_tag="IV247_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAP8777998.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2382..3179)
                     /gene="recX"
                     /locus_tag="IV247_001937"
     CDS             complement(2382..3179)
                     /gene="recX"
                     /locus_tag="IV247_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAP8777999.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            3286..4668
                     /gene="rlmD"
                     /locus_tag="IV247_001938"
     CDS             3286..4668
                     /gene="rlmD"
                     /locus_tag="IV247_001938"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP8778000.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4842..5420)
                     /locus_tag="IV247_001939"
     CDS             complement(4842..5420)
                     /locus_tag="IV247_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAP8778001.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5742..6212
                     /locus_tag="IV247_001940"
     CDS             5742..6212
                     /locus_tag="IV247_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAP8778002.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(6320..7546)
                     /locus_tag="IV247_001941"
     CDS             complement(6320..7546)
                     /locus_tag="IV247_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAP8778003.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(8300..8500)
                     /locus_tag="IV247_001942"
     CDS             complement(8300..8500)
                     /locus_tag="IV247_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8778004.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(8634..9242)
                     /locus_tag="IV247_001943"
     CDS             complement(8634..9242)
                     /locus_tag="IV247_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAP8778005.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(9327..9797)
                     /locus_tag="IV247_001944"
     CDS             complement(9327..9797)
                     /locus_tag="IV247_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAP8778006.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(9845..10759)
                     /locus_tag="IV247_001945"
     CDS             complement(9845..10759)
                     /locus_tag="IV247_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP8778007.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10905..11708
                     /locus_tag="IV247_001946"
     CDS             10905..11708
                     /locus_tag="IV247_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAP8778008.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            11886..12833
                     /locus_tag="IV247_001947"
     CDS             11886..12833
                     /locus_tag="IV247_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP8778009.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(12901..13194)
                     /locus_tag="IV247_001948"
     CDS             complement(12901..13194)
                     /locus_tag="IV247_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAP8778010.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(13222..13887)
                     /locus_tag="IV247_001949"
     CDS             complement(13222..13887)
                     /locus_tag="IV247_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAP8778011.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(14027..14659)
                     /locus_tag="IV247_001950"
     CDS             complement(14027..14659)
                     /locus_tag="IV247_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8778012.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(14666..15733)
                     /locus_tag="IV247_001951"
     CDS             complement(14666..15733)
                     /locus_tag="IV247_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8778013.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(15730..16461)
                     /locus_tag="IV247_001952"
     CDS             complement(15730..16461)
                     /locus_tag="IV247_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAP8778014.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(16630..17109)
                     /gene="greA"
                     /locus_tag="IV247_001953"
     CDS             complement(16630..17109)
                     /gene="greA"
                     /locus_tag="IV247_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAP8778015.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(17245..18420)
                     /gene="mltG"
                     /locus_tag="IV247_001954"
     CDS             complement(17245..18420)
                     /gene="mltG"
                     /locus_tag="IV247_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAP8778016.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
BASE COUNT         5529 a         3745 c         2984 g         6297 t
ORIGIN      
        1 tttgaaaact tgccatactt tttatttgtt ttattttcca gtatactata cgaaaggaag
       61 gtggctagac catgaaagtt gtacaaacaa aagatacaat gagcaatatt tacttagatg
      121 ctgtacggat caggcatcaa gtttttgtag tcgaacaggg tgtgcctttg tctcgagaaa
      181 tagacaaaga tgaagcccat tgtatccact tcgtcctgta ctcagataaa aaagaaccgc
      241 aaggaactgt acgtcttttg cctttagaaa acggaaaaat gaaattacag cggatggcga
      301 ttctttctga atacagacac caagggttag gaaaaatatt gattgaagaa gcagaaaact
      361 ttgctaagaa tcaaggctat aatacgattt tattaggggc acagtccaca gctgaaactt
      421 tttacgaaaa actaggatat acagcttacg gcgatccttt tgaagatgct ggcatgcctc
      481 atatttatat gaaaaagact ttataaaaat ttatttcgaa aaaagcaaaa aacagcaagc
      541 ttttgagaaa aatctctcac tggcttactg ttcttttttg tgtttactta cgcgacaatt
      601 gcttgtaacg gttataccaa atatcaatat agccttccga gaacgggcca tctccattat
      661 tgatccagtc aaccagaatc ttcacatttt cttttaggat gaagtcaata tcgttcgggt
      721 aattcatcat tttgctgtgc atctcatatt catcgacatc caatagtcgt ttctcgccgt
      781 caggaaatac cttgatatcc aagtcatagt cgatatattt caaggcctca tcgtccagca
      841 aaaaaggtga agctagatta caataatagg aaaccccctt ttctctgatc attgctatta
      901 cattgaacca gtatttttta tgaaaataaa caatcgctgg ttcgcgcgtt acccatcggc
      961 gtccatcaga ttcagtgact aatgtgtgat cattcacgcc gatcatagag tgttcgcttg
     1021 tttttaatac catggtatct cgccacgttc gatgcaattt gccgtcgtgc ttgtaacttt
     1081 ggatcgtcac aaactctcct tcttttggaa ctcccatagc aacccttcct taagcagcat
     1141 tttgtgcttt gtgtttgggt gttcattaca ccgacatcat aattattata tcaataaaaa
     1201 tgagctttgc ctagaaagga ttcctattgt ttcgattttt ctctaactga tctaccagtt
     1261 tcatttggac cttaggaaaa acgaccgaat caaatgcatc aggcttcaac cacttcgaag
     1321 ttttgttctc ggtaaattct tgttctgctc cctcggttgc ccgtccgtaa aaaaggagaa
     1381 catgccattt cagatgacta aaaatatgag taacttctcc taaatgtcgt gtctgccaaa
     1441 caaagagttc ctgcttttca aatgggagat tttgctcttc ttctgctact aggtcatcaa
     1501 acagatcgat ttctggcttt gtttcccatt cttttttcaa tcgctcatac tcctcttgtg
     1561 tcacttccat cattggaaat gtccacatat tcgccagcaa tttttggcta tctctttctt
     1621 caaaataata cgctccagaa tgattttgaa gagctgcact gatataataa acgtcttttg
     1681 gctttgcttt ttttgttttt acaggaaaac tcgtttggat tcctcgtttg ttagctaaac
     1741 aaaaggcttg gatcggacaa gtctcacact ttggagaagt tggtgtacag atagctgaac
     1801 ctagatccat cattgcttgg ttgaattcac ctggatgctt ttcatcaatg attttccgca
     1861 tcgcttcatc aaagattttc ctgctagaag cttttgcgat gtctgcttca atgcaaaaca
     1921 atctgctgac tactcgcatc acgttgccgt caactgcagg ttctggaagt ccaaatgcga
     1981 tacttgcaat cgcgcctgtc gtatatggtc ctattccctt caatgagcta atttcttcag
     2041 gcgtttgcgg catttcccca tcaaattctg acatgatctg tttagcagca gcttggatgt
     2101 tccgggctct tgagtaataa ccaaggccct cccatgcttt taaaagcttt tcttctggag
     2161 catttgctag ctcttcaatt gtaggaaacc attccatgaa acgataaaaa taatcgatca
     2221 ctgtgtctac tctggtttgc tgaagcatga tctcagagat ccagatacga taaggatcac
     2281 gattatagcg ccaaggcagg tttctttttt cttgttcata ccattgaatg aactgatctt
     2341 gaaattcttt tgtttcttta tctgtccatt tttcccattc tttattcact ttcctcttcc
     2401 ttttccgtaa tgaatcgctg gatcatatcc aaatcaaatc ctttttgata taagctttgc
     2461 tttactttta gatttctttt tttaggttca aaccttaaat ttcttcgcca tagtttatct
     2521 ccttgtttga ctaaataatc atattctatt tcttgatctg cttcttgcgg aagatcagct
     2581 atcgcttgtt ggatcacttc ttgagaaaag cctttcgtca tcaatgtttg ttggattttt
     2641 ctcaataatt ctttttggct ttttccgtga ttttttccaa acgctttttc ggctgtctga
     2701 tgtgcatttt gaacttggtc ttcaaatgta tattggctga gcgcttgttc aatgatttca
     2761 ggtttcaacc ctttttgctg catctgctgc gctaatttct ttggtccttt gtcacttaag
     2821 cgcatattcg tgcgcacata actttcccca tagaccaaat catcgacaag gtccaattct
     2881 tttaagcggg ctacgatttt atttcgatct tcttgaagga tttccttttc ttttaagtat
     2941 gttcggattt ctttttccga tcgcaactga taacttaagt aattcattgc taggtgaaaa
     3001 ccgaaatcag ctccgctact cttcttgatc tcttggatag tttcttcgtc taattcccgt
     3061 ccttttaata atcgatagcg tactaataca tcttcagata cttgtaaagc ttctccttca
     3121 gataaatcta cgcgatagaa agggcctttg cctttactga ttctcgtaac ggttaacata
     3181 aacatcctcc tcgaacatat attctctatc ataccacaga aaatccccgt aatcaggtat
     3241 tatatgttag aataaaccac agaaaaaaga aaaagggaga tacttatggc agaaacaatt
     3301 gtcaaaactg gacaaacggt tcctttgaaa atcaagcgtt taggcataaa tggtgaagga
     3361 atcggttatt tcaaacggtt gatcatattt gttccatatg cactaccaaa agaagaagta
     3421 ctagtgaaaa tcacgaaagc aactcctcgc tatgcagaag gagaattaat aaaagtcaaa
     3481 aaaacaagca aagatcgcgt agtggcacca tgtcctgttt actatgaatg cggtggctgt
     3541 caattacaac acttagctta tcaagctcaa ttggatttta aaaaggatct gcttttgcaa
     3601 gcattggaaa aattcaaacc agcaggcttt cgatcttatc aacttttgcc gacgatcggg
     3661 atggatgagc cttggcatta tagaaacaaa gcacaatttc agttacgaaa aaataagcag
     3721 actcaaaaaa ttgaagctgg tttatacgaa gcaaactctc atcagttagt ccctataaca
     3781 gactgcttgg ttcaagaacc tcgaacacaa aaagtaatga atactgttgt tcaactattg
     3841 aataaatttg atgcacccat ctatgatgaa cggacaaaca gtggaatctt ccgcacaatc
     3901 atggttagaa tcggtgttaa aactggggag ttacaagttg tttttattac tcgcagtaaa
     3961 aaattcccgc aaaaaaacgc aatgatccgt gagatcaatg aacgactgcc cgaagttgtt
     4021 tcaatcatgc aaaatgtcca ggcacaaaaa acgtctctcg tgatggggga tgaaacgatc
     4081 cacttatggg gaaaagaagc cattgaagaa caaatcaatg aagtgacttt cgatctttct
     4141 cctcgtgcct tctttcaact caatcctcag cagaccgaat tgctttatgg cgaaggaatc
     4201 aaagcattag atgtccagcc taatgaaacg atcgtggatg cttattgcgg agttgggacg
     4261 atcggcttaa gtgtagcaaa acaagccaaa gaagtacggg gaatggatac cgttcctcaa
     4321 gcaatcgctg atgcaaaaaa aaatgcggaa agattaggat atcacaacac tcgatatgaa
     4381 gtcgggacag cagagcaagt tctgcctaaa tggttgaatg aaggttttca gcctgatggt
     4441 atcatcgtag atccaccacg tacgggtttg gataacgcat tgatccaatc attattgaaa
     4501 taccctccaa aaaaacttgt ttatatctcc tgtaatgttt ctacacttgc cagagatctg
     4561 gttaatttaa gtaaaaagtt caagattgaa tatttacagt ccgtcgacat gtttcctcag
     4621 actgcacgtt gtgaagttgt cgtgaagatg acgagactcg ctgactaaat gaaagaggtt
     4681 gtgaaaaagt ctgccctgta tcaagtaata agaacagcca aacaaaaata gctcttcatg
     4741 ttttatgaat actgtacttg tgaccgaaga gttaacagga ggacactatt tatttaaaaa
     4801 aagggttgtg acatagtttt tgtcacagcc ctttttgatt attattggat attcacgtct
     4861 atcacttgat aaaatgcatt tgtcgtatcg ctgatattcc atactgctaa aatcacatta
     4921 tatccttttc gatcctgcgg aagtgtgatc gtttggttga ctgtttttgg tggaacagtt
     4981 gctttgtcgt ctatttttgt gatcaattca aaatcactaa atttcaaagg ttgattagga
     5041 ttccaagttg gtttggtaat gtagtaatcc caagtacttg tccgatgttg tgctgttaaa
     5101 ttccaagtaa tatttaatgt tcctggatta accatgtttt tatgccatct gttagatgtc
     5161 tgttcatcta aaggctcaaa gccgctgact cctgcactag caatttttcc atcaatgaat
     5221 gtatttttgg tagcttcgat actttgcggt tcccattgtg ctcttccgac attttgattt
     5281 aagtttcctg ctgctgatgt tcctaggtat gcacggcttc ctggagaagt aatataacca
     5341 tgcgcaaaac ctggaatgct aaaaccgaac aaccctaaac taactaaccc aataaacaat
     5401 aacgtactaa tctttttcat gtagaaacct ccttcatttg ataaccacat tgtaaacgct
     5461 ttattgtatt aacacaaaca ttatgctttt ataattttat gttgaagtaa aaaaggtgaa
     5521 aaaagtaaga tgcagattat aacagaagta gacgagaaaa gtacaaatag aaaacatgag
     5581 aatcaaatca tatgatcttt ttcaaaaaca tcattcatat aaacaaatgt tcaacttttt
     5641 aataacaaca attatcaatt tttttcttac ttcacaaact aaatacaggt cttgctgtga
     5701 gccttcatgg taaactaata ttatagaaag gagttagtaa aatgaagatg ataaaagata
     5761 ttcgtttttg ggtttgtgtt ataggaattg tcatattagg ctttttatca gggttgctta
     5821 gcggaaatcc cggaaaatat tactattctt tgcaacttcc accatttgca ccacctagct
     5881 ggatatttgg gccgatgtgg accttactgt atatattgat gggtatttct ctttatttgt
     5941 tacttaatca caacaacaaa aaacagcgaa acaatcttgt cggtcttttt gtgattcagt
     6001 tcatttttaa ttttatatgg tctgctctat tttttaattt aagaaatatt ttcattgcag
     6061 caatcgatat tacactttta gttattttct tgtcagtgct tatgtatcag ttgtggctgc
     6121 atcatcgcct ggcaatgtgg ctcatgattc cttattattt atgggtacta ttcgcaactc
     6181 tattgaatta ttcaatttat tttttaaact aagcgctaaa aagagacgtc ctgtaattta
     6241 acacatagaa aaagtgccac ctaagttaga ttttttgtct aatttaggtg gcactcttta
     6301 ttaaccaaac tatttttttc tagtacccac ttgttgttcc atactcatta ttctggtcat
     6361 aaccataacc attttgttcc tgtccagcgt aaggatcata actttgagta tcttgacttg
     6421 gtgtatagta ctgacttgta tcttctgttt gctgataata ggtattcgga tcttggtact
     6481 gatcatatgt gttttctgaa taggaagaat aatcggtgta cccttcacta ttcgatccat
     6541 caccgtttgt cgaatcgcca tcttccgaat catcaaagaa catcattgaa gcattctcat
     6601 ttttcaaatt aggtaatgta tcgcttgttt ttatatttaa ttgtttcttc agttcatttt
     6661 ggatcgaaag aagatcattt tttcctaaaa tttgatagta gatatcattg atcatttgtc
     6721 cttcgccttg caactggtct tgtttgattg tttcaaacgc cggggtgtag ttcgtcgcta
     6781 catcaagcat gtcatcccaa tctaagtccg tcgtcacatt gttctcaact gcttttaaaa
     6841 tttttctata attgcttacg ccatctaaac ttaaaacttt attgacgatt ttcgtcacta
     6901 cttcccgctg acgacgttgt cgaccgatat cgccttctgg atcttcgtgt cgcattcgag
     6961 aataggctaa acctttttct ccattcaatg tttgttcacc tactggtaca tggacaccat
     7021 ccaaagtaaa gtcaatttta ttattcactt tgatgccacc gaccgcattg atcaaatctt
     7081 ccatgccatc caaattgatc gttacataat gatcaatagg aatatcgagc agttgctcaa
     7141 ttgaatccat ggccatttcg acaccaccaa atgcgtaggc atgatttaat ttatctaccg
     7201 ttccataacc tacgatattc gtataaatat ctcggtccaa actgatgatc gttgattttt
     7261 tttgtttcgg attaactgtt actaccatca tcgtatcaga acgtccttgt tccgttcttc
     7321 ctaatccacc ggtatctaaa cctaatagca aaaccgaaaa aggttctttg tcatcaatgc
     7381 ttactctctc agaacgtttg gaggatattc gattagagtt tttagaaatt ttgtttactg
     7441 tttggctcgc ttctcccata aatctgattc cgtatgctga taatccggca atcagcaaaa
     7501 cgaatacccc taacaaggtt aatagaactt tttgccatcg cttcataata cacctctgtt
     7561 tcacattttc taaaatattg tctcatataa ctgttcaatc agcaaattca ctttttaatc
     7621 ataagaaaac tttaaaaaac atttttgtgt aacgattatt attttttaaa atttctcaaa
     7681 attcttaact atcataccat accgtgaaaa agatcgaaag accgaataca cgcagttcgc
     7741 aatcgtttta tcttttttta tgtatttttt ccagaaaaag cgaacaacaa ggtattttcc
     7801 aaataatcgt gcagtaaaat gatcgcacat acctttccca tctttttaac agtctttgcc
     7861 taccgttcag attatcattc gatttcacga caatttcttc ctatgactgg tccttttgtt
     7921 aaacccatct tctattcgtt ttatttccag ctcactgcat ggctcttaaa atgactcgaa
     7981 ctagtgattg cattaccatc atcttttaat ggatagatat gctcctcttc aaagtaaaac
     8041 gattttcttt taaagtaata gaattgacat ggtaatgagt atgtatacta gtggtttcac
     8101 aaatggtctt ttattcaaca tccaaaaact gaaatttctt taaaatccat tactaacgaa
     8161 gaaaatgttc cttgcttgat aattagttag gacatgtcaa gtgaacaaat gcatttactt
     8221 agccaaaaaa tctaaaaact tcataaatag acaaaaagac acgactctct aagaagtcgt
     8281 gtctttattc aacaaattat tataatttag tcacttcagc agcttgaggt ccgcgtgcac
     8341 cttcaacaat cgtgaattct acttcttgac cttcttctag gctcttgaag ccgtctcctt
     8401 gaattgctga gaaatgaaca aatacgtcat ctccgccgtc tactgtaata aacccaaatc
     8461 ctttttcatt gttgaaccat ttaaccgttc cgtgttccat aaatgtatat cctccaaaaa
     8521 agttcttgca agtaaccttg caagaacatt atacccaatt tagaaaacgc tagcaagctt
     8581 tcgctctttt attttccaaa ttatttttaa gactgatatg acggaggaaa tggttatttt
     8641 gctgcttcga aacgtttgtt tacttcatcc caattaataa cattccagaa tgctgcaata
     8701 taatctggtc ggacattttt atatttcaaa taataagcat gttcccaaac gtccaaacct
     8761 agaattggtg tctttccttc catcaatgga gaatcttgat ttgcagtaga ggtaatagct
     8821 aattttccat tttccattac aagccaagcc catccagaac caaatcgtcc agcggctgct
     8881 tttttgaatt cttctttaaa agaagaaaaa tcaccaaaag cttcattaat cgcttctttt
     8941 atttctcctg taggttcgcc accagcattt ggtgccatga tttcccagaa aaatgaatgg
     9001 ttagcatgtc cgccaccatt gttacgtaca gctgtcttga tatctgttgg aatagcgtcc
     9061 atatcagata ataattcttc tattgttttt tcgcctaatt ctgggtattt ctcaattgct
     9121 gaatttaaat tcgtcacata agtattgtga tgcttatcat gatgcagatg catcgtttct
     9181 tcgtcaatat aaggttccaa tgcgtcatat gcataaggta gatctggtaa tgtgtaagtc
     9241 ataaaaaaat tcctcctcaa tgttttctta ataatgtcta cattacaaag agtaacaaag
     9301 agaaggaaat tcttcaattg ttatgcctag tcgtcttttt cactttgacc tacttcttct
     9361 tcaaaaacct cagtttcttt aaaaataaac aacttgctga aaatatagtt cgcaaggata
     9421 acaataattt gagtgatgat tttcgccaca tactcattgc tgttcataac attgatcaac
     9481 aataccattg cccccatgtc caatccgtaa gaaaggatac ggtagaagaa gaacttaccg
     9541 cattcaataa gtgcatgtgt aaaattcggt gattttgaat ggaataccca aagtttgttc
     9601 gttacaaaag caaacaatac agaacaaaac cacgaaatag aattggataa ggccatactt
     9661 aaatcaaaat attggcgtgc gagagcaaaa ctaacaaaat ttacaatcgt agccaaacca
     9721 ccaaaaaaga gatagactag tatctcccaa tatccttttg cttctaaata tccttttaac
     9781 tgtcgatact gcttcatgag ccaacctacc ttattaaaaa ttcttattta tcatactcta
     9841 tatttcaata actttcatct ttatttaaaa aaattaattt tttttgatcc tttgcctttg
     9901 tctctgcttg gatcgtcaca tgtgtaatac catattttaa ttttaataaa gaaattatac
     9961 gaggatatat cttttcaatc tcgcttagta gctgatcttc aaaatcaata tgtccgtcga
    10021 aaataattat cttttcgcta tactgccaaa tatggacatg atgaacatcc tttaccccat
    10081 cgattgcttc aatttcttgt tccatttttt catagtcgat agcaggtgca ctttgtatca
    10141 aaatagcggc agcttttttg atcaccatta tcgcttctat caatatatat atcgaaatga
    10201 tcagtgtaat aactggatcg atccagtaga tatcgaaaaa acggatcaaa attgctccga
    10261 taatcacact taccgaagac agtgtatcac ttaacaaatg caaataacta gattttatat
    10321 tcaaactttc atctgcttct tgtcgtaata ataatactga gaacaaattc gcgagcaatc
    10381 caataactgc tactgttagc atcaaatgac tatttatctt ttctggtgaa gtgaaccgct
    10441 tgaatgcttc aacggttaaa aacaaggata atatgatcaa tatgaatgag ttgatgaaag
    10501 cactcaatat ttctaaacgc tggtagccat acgtacgttt tttatttgct tcttttcggg
    10561 ccattttttg tgccccgtaa gaaaaaataa ttgccaatgt atcactaagg ttatggaaag
    10621 catctgaaac aagagacaaa ctccctgaca taatccctcc aataaattct gataaagtga
    10681 tcaccgcatt caaaaccact actccaaata gtttttttga tgatctctgt ggctgtgtag
    10741 gcactattcc ttcctccatt ttatttatat tggatagtct ttagacaaga ataacatttt
    10801 tttgatattt caccaatcat tcagctgttt ccttaaagca cttgtatttc acttgatact
    10861 tcgctagaat tgcaagtgat gacactacta gatgaggagt acttatgaca acaaaacaat
    10921 taattatcag ttcctttata cgattcaaag acctaaagga ggcgagaaat gtcccatttt
    10981 ggaaagccat tgtctatctt ttagttttga gtataattat ggctttgcca atctcttttc
    11041 aagtttttca agtcttaaac aacattcaat tggatgggca aaagattgct actcgcattc
    11101 ctgattttac aatccaaaac gggcagcttc aaaccgaaga aaaagaagga ttcatttacc
    11161 aaacaaactc cattatcttc acatttgatc cagagggaaa acgaacagaa caggacatct
    11221 ctactgattt gatggggaat tttgttagtg tcgggatgtt aaaaaacaaa cttatcatag
    11281 ctcttcctaa tacaggcacg acttctgctt tgttgaacaa taaccaatta gaacttccct
    11341 acaccaatga atctttaaaa aacttaacag gtaagcagct ccgttctttt ttaagtgaag
    11401 catctatacc actttggata aaagtcctga catttatatt ttctatttat cctagttttc
    11461 tgaatctgct gatcactttg ctatttacca acattgctgc ttttctctat gcacgctttc
    11521 gactaacaaa agctactttt cttgattgtt taaaaacatt gatctattca atttcattgc
    11581 ctactataat tgcaacaatc ttgatggtct ttcttccttc ttttgacact agcgccttca
    11641 ttgcttttgc aggaatcttt atttttgctc aagctgtcaa aggatgggca aaaatatcga
    11701 tcaattaaga aataaagaca actcgttatt tctacttcaa tagacccttt tagaggttgt
    11761 gacatttttt tgtcacagcc ccttttttat ttgaaaaata tttctttctc aaaaaacaag
    11821 caaggcgctt caaagttctt cttgtatgcg ctacacttag agtaaagaaa ggcgggaaat
    11881 taactatgtc agaaagaatc atttatgcag atcgtccatt tcgtgatgaa tttatcgaag
    11941 aaatccatac tatagcgcct ggttatatct ttaaaacaga attacaacca gaggatctac
    12001 aaaatgtaga gatcagtttg ggctggaact cagactacca agaaaaacta ttagcctctt
    12061 ctaccttgaa atgggtccaa tctatctcag ctggtgttga tcagctaccc cttgagactt
    12121 tttcaaagaa acatatttta ctttcaaatg gtagtggcat ccacagtgaa tcgattacgg
    12181 agcatgtctt aggaatcatc cttgggtatt ctcgtggtct ttttcaagca caaaaggcac
    12241 agagtgacat gaaatggtta ggttcatcgg tccattacca atctatccaa gggaaaaaaa
    12301 tactaatcat tggaactggc catattggaa aaatgattgc aaaaaaatgc aaagcattag
    12361 acatgaactg ctatggaatc aatacatccg gtcatccagc agaaggaatg gaaaagactt
    12421 tttctttaaa tcaactaaag gaagttcttc ctgaagtaga tttcgtagtc aatatcttac
    12481 ctctaacgga ggaaacaact gggctctttg atcagcatat gtttgaacac tttgatccaa
    12541 aatctgtatt catcaatgtt ggccgtggtg cttctgttaa gacacaagat ctggtgcagg
    12601 ctttaaataa ccagcaactt tcctttgcag cattagacgt ttttgaggaa gagcctcttc
    12661 cacaagatca tcctctttgg aaaatggaca atgtactgat tacttcccac attgcaggtt
    12721 tgacacctga ttttcaaaag aaattaatgg ctattttttt aaccaatctg aaaagctact
    12781 ttgccaccca tgagcttcaa accaatcaag taaaattgtc tgcaggatat taatagctga
    12841 aaacaatatt ttcaaaagaa aaaagagaag gtgacaaaaa ttgtgtcacc ctctcttttt
    12901 ttattgtttt ttaaagtgat aggttggctc ctccaggttg ggatcataat caacaagtaa
    12961 atcatatcct ttaaagaacc attcatctgc ttcctcgata aaaaaaagtg tcccatcaat
    13021 ttccactttc tttacaggat gttctggctg ttcaactgac attccaatcg aaaaaccatc
    13081 atgaacctct gttttgccgt agacttttcc aaaaaaacgt attccgtaag tttctggcag
    13141 atcaatttct gagtcaaacc acttttgtgc ttttggcgtt accgttaatt tcatcccaac
    13201 aacctacttt ctcttttctt tctattgatt catcttatcg tctagaatat ccacatcgcc
    13261 tgtctctcca ataaccagta gattatcatt ttcacgaaca tattcatctg ctgcaggaga
    13321 agcgataacc gtttgatcta ctcttcgtat ggcgactact gttaagtcaa aacgttgcct
    13381 gaaatttaat tcagctaatg ttttgttgaa aaatttagga ttggagactc ggatttctgc
    13441 taaagaatat ttatctgaca actctaggta atctaaaata ttctttgaca ctaaattatg
    13501 cgcaatcctt attcccatgt ctcgctcggg atggacgacg cgatcagctc cgatcttttc
    13561 aagtactctg gcatgatact cattttgggc ttttgctaaa atatttggta cgcccatttc
    13621 ctttaccatc agagtaacta agatactggc ttgaatatct tctccaatag cgacaattac
    13681 atgatcgaaa tttcttatgc ctaatgatcg aagtgtcatt tcatcctgtg catttgccac
    13741 aacagcatga gtggctatat tcatatattc attgacccga tcttcattac tatcaatagc
    13801 cagtacttct tgaccagctt cgatcagtgt tcggcagata cttccgccaa atcgtccaag
    13861 acctacaatt gcaaaatttt gtttcaccgt tttcctcctt tagtatcttc ttttctcagt
    13921 ataacaaagc ctttacaaaa acaaaagcaa gcatcattat tgtaaaaaag ccaagaaaaa
    13981 taatagttat tttctcagct tttgattatc ctctttatca tcgtttttat ttcaccaaac
    14041 catgtttgaa tgcatagata gcggcttgtg tacggtcttc cacttctagc ttagccaata
    14101 tattggaaac atgtgttttt actgttttca gtgtaataaa caattcgtcc gctatttcct
    14161 gattactttt tccttcagaa atcagcatta aaatttcatt ttcccgattc gttagttctt
    14221 catgtaatac aggctcattt cgccgactca ttttttccat catttttgtt gtcacttctg
    14281 gttctaatac tcgctcgcca cgttgcgttg cccttatagc atcagcaatt tcatgtgcgg
    14341 tagaggtctt caacaaatac cctgctgcac ctgcttcaat ggcaggataa actttttcgt
    14401 catcaataaa actggttacg ataatgattt tagcttctgg ccaatctttt aagattgctt
    14461 ttgttgattc gataccatcc atttcttcca tgactaagtc cattaaaatg acgtccggtc
    14521 gaagtgtcat cgccttctca tagccttgtc gtccattttc agcttctcca attacttcaa
    14581 tgtcttcttg aatagataaa taagaagaga cgcctaagcg caccatttca tggtcatcta
    14641 ctaataaaac ttttatcatc cttatctatg cctccttcat caaagggacc ttgatttcta
    14701 cacttgtacc ttgaccttta aaactaatga tttttactgt tccaccaata ccagctactc
    14761 gttctttgat attgttcaat ccataacttc ccgttttcgt ttcattcata ttaaaccctg
    14821 ttccgtcgtc aataatacgt aacaaaagat tattgtctat tttgtgcaaa tatacctcta
    14881 attcattggc tttagcatgt cttaatgtat ttgataacaa ttcttgaacg attcggaaca
    14941 agtgatcctc aatggaacta gatagtttta catcttctac atcccatttc agcgaaatct
    15001 ggattttatt ttgcaattcc ttcaataact gctcgatacc ttgctttaaa cttttctctt
    15061 ctaaattgac tggacgtaga tgaagaagca gcgcacgcat ctcagactgg gaagcgttga
    15121 tgatctctgc taccattttc aactgcttgc gaaacatttc tggtgtctcg cttttttctg
    15181 cttgctccgt caatgccgac atcatcatca ttgctgcaaa caattgttgc gaaactgaat
    15241 catgcagctc acgggctaat cgatgtcttt ctaattctaa aatttcttct ttggtttgtc
    15301 catctacata ttggggacgg ctcgttacaa tttgtaattc actagatact tcaatcattt
    15361 tttcttttat cttagtgatt tccttatcaa tatctttgat gtataaatcg tccgatgcac
    15421 ttgggatcgg aagaagcaac aatttggatt cataattgcc agaagaaagt gcacgcagct
    15481 tttcttcgat tttcccgtat tttgtctttt gaataagtga taataataga aagacaatta
    15541 atcctacacc taaggaaatg gctaacaaat ggaagataag cggaacatat aataaacgtg
    15601 cacgcagtaa ttctccccac caatgacttt gattgctggc ataaaaataa gtgaaaagtg
    15661 aaaatagaat aataaggaaa gccgcaatcc ccgaataaac agctagcatc gctctggata
    15721 tttttcccat catattcgta tcacctcgac atctccaatc aaggtattag taataatctt
    15781 caatctacga gggttttcat cataatcact gctgtagatt ttgatttgtt catttttcaa
    15841 agaaaattgc tcttcttcaa acaagacatt tcctactaat gtcgcatgct ctaatcggat
    15901 ggctacgccc aaaggtacta agatccgtgt tctgcctatc ccttttctta caatcacaat
    15961 attatcatct tttggcaaaa gcgtattgcc caaatctatg atagtatcac cagaaatgac
    16021 ggcgatgttg atatcgtccc attcatagac gtcgtttcca atacgttggt taccgaataa
    16081 ttgctgtctt ttccgctcat tattatgtgt ttttaattgc tctgtttgga tcatcatgat
    16141 ctgtttcttt cgccaaaaag catttttggt taaatctatc cctgaaatct ctacaccttt
    16201 cagaccaata aataaaacgg caaatacagc catcatccat aaagcaggac tgttgactaa
    16261 gctaaaaaaa atgaccaaac ttcctaatat caattgaaaa ttttgaaatt tagtacgagg
    16321 atgtttgcgc atagctagat atatattgaa aataccgaat atcactaaaa gcaaaagtcc
    16381 agtattgttt acgatttgcc aaacagcaaa taacagcagt agtgcttcaa ctaccacaaa
    16441 aaaacgccaa gaactattca tcgctctttc tccctcctga cacattctca tttcatcata
    16501 cctatttttt taactgttgg ctataggact ttagatgtaa gatcgtttgt tgaaatctaa
    16561 aatacacaaa gagaaaaaga tctagagtgt atcatcagta agatgaactc tctagatctt
    16621 tcagtcagac tacgcttgtt ctactttgac gattttaact aacatatcgc cacctggagt
    16681 agcaatagtt acttcatcat ttttacgttt gccaatcaat gcttgcgcaa taggagaatc
    16741 attggaaatt ttgccagaaa acggatctgc ttcagcgctt ccgacaatcg tgtactcttc
    16801 ttcttctcca tctggtagtt cgatgaatgt aactgtttta ccaattgata cctcatctgc
    16861 atctacaccg tcgttatcga tgatttgagc aaaacgaatc atattttcaa ttgttgtgat
    16921 tcttccttca acaaatgctt gttcatcttt cgctgattca tattcagagt tttcggaaag
    16981 atctccgaaa ccgcgggcaa ttttgattcg ttcaatgatt tctccgcgtt tgactgtttt
    17041 tagttcttct aattcttttt ctaatttttc tttaccttct agagtcattg gataaacttt
    17101 ctctaccatg tgtaacgtct ccttttttcg ataaaatacc gcaaaaggta atcttttgag
    17161 gaatatagtc caaaaaagat taccacgctt ttcttcaaat gtaaatgttt ttctaaaaat
    17221 gatcaatgtc tagagcctgt ttttctaaga attatttaca tatttttcca ctaattccat
    17281 atgttcttca taagttttag cgaaataaac atttccagtg ctcgtatctg ctacgaaata
    17341 ataatagtca ttttctgctg gttctaatac tgccttgatt gcctcttcgc ttggattgtc
    17401 aaaaggtcca ggtccatagc cggtattggt atacaaatta tatggtgaat ctacagctgt
    17461 atcttcatac gtcactaatt ctttgtgttc acctaatgcg tataaaacag agatatctga
    17521 ctgaagtggc atatttgctt tgattcggtt aaagaagact tgtgcaatgt ttttacgatc
    17581 attttctttt acgccttctt tttcaactag agaagctaga gttaaaactt cttgtacagt
    17641 caaatttttc tgcttgatag ctgaataata gttttgcatg accgtattcg tcttgttcac
    17701 catttcaatc actagatcct taagcgaagt actcgtgtaa taatcatatg tggctgggaa
    17761 taagtaacct tccaaacggt attttgtgtt ctcagcttta gatgcacttt ccaacagttc
    17821 tgggaatttt tggtgcaact cgttaaagaa agtctcatca ttcactaaat ctaaaaattc
    17881 tttcttgtct ttaccagtta ccttcgctac ccgttcagca atctgatcga tatcgtaccc
    17941 ttctggaatg gcaattttcc catctgcaac ttttgttggc tcagaagtac cgccttcttg
    18001 caactgtttg ccaatctcgt caagagtcat attaggcgct aactgataat agccagcttg
    18061 aaagccagtt aaatttttaa attttgtata ataattgaaa acaatcccgc ttttaataat
    18121 attgtccttc tccaaaatct caccaatctg cttgttagaa gagccactcg ggatctcgac
    18181 ttggactaat tggtcatctg atttatctaa aggcttcaac ccgctatcta cataacgata
    18241 cacggtaaat ccaagaaatc cgccaataat caataatgta agagcaatca ctaaaatgat
    18301 cttcctcaca atcttatctt cttttttacg cagatttctc cgcttgtcga ttatatctgc
    18361 tgtagctgcc ttactggctt cttttttatc tttacttggg tgttgtttgt ttcctgacaa
    18421 aacaactcct ccttactatc aagttcgtaa ctttattcat tatacatgat atttgaattg
    18481 gcgtgaatga ttttatttaa atattctatc aaaaatgtaa gtagtaaatc cattactata
    18541 agattaaaat aaatg
//
LOCUS       DACDIG010000053        18377 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.053, whole genome shotgun sequence.
ACCESSION   DACDIG010000053 DACDIG010000000
VERSION     DACDIG010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.053"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            101..1129
                     /locus_tag="IV247_001955"
     CDS             101..1129
                     /locus_tag="IV247_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778017.1"
                     /translation="MYWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWIS
                     LLRISLQRMKQGHSITYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTE
                     QAYLYFMFGVMNTYLEEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLY
                     VNLAMIHLYSRVFGTKKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNT
                     YYEKNRRLIFQYCMLVRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYS
                     ASADQLPDIVISDYPIDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSI
                     LEKLDFVN"
     gene            complement(1155..1595)
                     /locus_tag="IV247_001956"
     CDS             complement(1155..1595)
                     /locus_tag="IV247_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP8778018.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            1755..2261
                     /locus_tag="IV247_001957"
     CDS             1755..2261
                     /locus_tag="IV247_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8778019.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            2429..4342
                     /locus_tag="IV247_001958"
     CDS             2429..4342
                     /locus_tag="IV247_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP8778020.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            4347..5348
                     /locus_tag="IV247_001959"
     CDS             4347..5348
                     /locus_tag="IV247_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8778021.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(5550..5756)
                     /locus_tag="IV247_001960"
     CDS             complement(5550..5756)
                     /locus_tag="IV247_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAP8778022.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(6402..7250)
                     /locus_tag="IV247_001961"
     CDS             complement(6402..7250)
                     /locus_tag="IV247_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8778023.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            7414..8316
                     /gene="gnd"
                     /locus_tag="IV247_001962"
     CDS             7414..8316
                     /gene="gnd"
                     /locus_tag="IV247_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAP8778024.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            8318..9319
                     /locus_tag="IV247_001963"
     CDS             8318..9319
                     /locus_tag="IV247_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP8778025.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            9316..9600
                     /locus_tag="IV247_001964"
     CDS             9316..9600
                     /locus_tag="IV247_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8778026.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            9633..10430
                     /locus_tag="IV247_001965"
     CDS             9633..10430
                     /locus_tag="IV247_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8778027.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            10444..11277
                     /locus_tag="IV247_001966"
     CDS             10444..11277
                     /locus_tag="IV247_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8778028.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            11295..12152
                     /locus_tag="IV247_001967"
     CDS             11295..12152
                     /locus_tag="IV247_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8778029.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            12174..12683
                     /locus_tag="IV247_001968"
     CDS             12174..12683
                     /locus_tag="IV247_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8778030.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            12720..13121
                     /locus_tag="IV247_001969"
     CDS             12720..13121
                     /locus_tag="IV247_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778031.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            13233..13586
                     /locus_tag="IV247_001970"
     CDS             13233..13586
                     /locus_tag="IV247_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778032.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            13620..14063
                     /locus_tag="IV247_001971"
     CDS             13620..14063
                     /locus_tag="IV247_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP8778033.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            14080..15165
                     /gene="uxuA"
                     /locus_tag="IV247_001972"
     CDS             14080..15165
                     /gene="uxuA"
                     /locus_tag="IV247_001972"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP8778034.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            15158..15871
                     /locus_tag="IV247_001973"
     CDS             15158..15871
                     /locus_tag="IV247_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8778035.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            16623..16823
                     /locus_tag="IV247_001974"
     CDS             16623..16823
                     /locus_tag="IV247_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8778036.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            complement(16927..18228)
                     /locus_tag="IV247_001975"
     CDS             complement(16927..18228)
                     /locus_tag="IV247_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP8778037.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT         6303 a         2906 c         3837 g         5331 t
ORIGIN      
        1 acttaatact attttgaaaa actatcattt atcttgcagc ttgaaaaaag gtaatccgat
       61 agaaggagaa gaataccgta tccgatactt ttttcacttg atgtattggc agtcattcga
      121 cgaaaatcat tctgatatat tatcaagtat aaaaaataaa cgtcaactat tagaaaaatt
      181 tgcgaaacat gcgccatata cgcgtagtat agatagaaag aaatggatca gcctactaag
      241 aatttcgttg caacgaatga aacaaggcca ctcgattacg tatgtcccag aagagatgaa
      301 caaatttgtg catccattga taacaaaaga agagtttacg cattacatac tagagccttt
      361 ttttgaagca aacttctggt cgatgaatca catcactcca acagagcaag cttaccttta
      421 ttttatgttt ggtgtaatga acacctattt ggaagaagat ttgttggata ctccagataa
      481 agtacaagaa agttggggct ggattgaatc gtctattgta gaaaattttg aaaaaaactt
      541 caagatttgt tttacaactt cagaaaaaaa ttatctatat gtcaatttgg caatgatcca
      601 tttgtattca agggtttttg ggacaaaaaa gaaggtggat gcttttggaa aatcagcaga
      661 agacagagat tttcagcata tatttccagt gatgtatcca atcatgcaac agttttttag
      721 tggggtagct cgcagaattc cagagcttaa tacttattat gaaaagaaca gacggttgat
      781 ttttcaatac tgtatgttag tcagggatat ttttataaaa cacgaacaac ctgtcatatt
      841 ctatattcaa tcaaagtatg gtaaaacaca ggaattatgg gcaaagaaaa ggatattatc
      901 tatgactgaa cgcccaataa aatatagtgc atccgctgat cagttacctg atattgtcat
      961 tagcgattat cctatcgaca aaaaggcata tgatcaaacg aatacacaga tattttactg
     1021 gaatggacaa cctacaaaaa atgattgggc gcgtctgaaa gctgaaatca taaaaatcag
     1081 gaatgagaaa aatagatcca tattggaaaa attggatttc gttaattaag aaaagcagat
     1141 tgtttatgcc aaaattaaga cattgcaaaa agattatttt ctttaaaatc gacaagatga
     1201 ccagaaagca agagttcgtt gagctcttgc tttcttttag ttttagaaag gaacatcctg
     1261 attttatctt cgctaaatcc agtcaaaagt tttgcagatt ggtcgtagat aatcggtttt
     1321 ttcaataaag aaggggatgt acgaatctca atatatagtt cactcaaact gagttcatct
     1381 attctgtgaa caagattttt ataactaata gaatgagtag agagcatttc ggtcacgccg
     1441 ttgtttgtca attttagtaa gtggataaat tcatttttgg ttagtggttg acgaattaag
     1501 ttctgttcga taaacggtat atcatggacg ataagccaac gttttgcgcg tcttgaagac
     1561 gagcagccag aacctgtata caatttaatc atcatacatt cctccaatag ctaagtattt
     1621 atgaaataat aacatgaaga aatagtcgaa aagtgccatt tagaggtgta aaataagaaa
     1681 atgttgtcac gtataatgga actagctaga taagaaatac tattaaatca agaaaaaaga
     1741 aagaaggcac aagtatgtca aacacagccg acgcaatagt agtgatcgat ttacaaaaag
     1801 gggtatgttt tgattcttcg ccgatacacg aattagaaac agtgacagca ttagtgaata
     1861 aaagaatcaa aacatacgaa acgattggga aaccagttat tttcgttcaa cacgaagatg
     1921 ctcaattagt gccacaaaca aaaccatggg agctattgtc ggaattatct attcctgagc
     1981 acagttattt tgtgagaaaa acccatgcga attcatttta taagactaat ttgagtgttt
     2041 tattaactga attggactgt cattcgatcg aattatgtgg ggcacaagca gagtattgta
     2101 tagatacgac tgtgaagttt gctcatggat taggatacca gcttatgatg tgtagaggag
     2161 caacgacaac ttatgataat ccttttatga aagcaaagga aacaatcgct ttttacgaga
     2221 aaatttggga taaacgattt ctgacatttt tggagcaata agttgtttct tcgtttaaaa
     2281 aaagcagaat ggatactggt ttatccattc tgctttatta cgtaaaatga gaaaataatc
     2341 ctgttctatt tttatgggat aaaccattta ttttttgtat aaaaaatgtt tgcgtttaca
     2401 tataattctc ctagaaacat aaaaaggagt gaatagcttg agaacaaaac aagtattaaa
     2461 gggagcgttg gtcatcttat ttgctgtaag tacaagcgtc ccgcaatgga cagtatatgg
     2521 agagagcatt tcttccgaat cggaatctcg ggaggtagaa accacagaaa ctactgatgc
     2581 tggaaatgaa acgaaatcga gtacttcaca aacagaaaca aatactgaaa caacggttga
     2641 gacatctgtt actagcacag atcaagaaac gaccgctacc acagataacc aacaaacaga
     2701 agaagcaaca gctacaaaaa tgtcaaacga cgaatcctct tctgctaaag taagtgacaa
     2761 agagaatggg acagaaaatg atgatatcgg taaaggtgaa aatttcaatg gtaaagaata
     2821 tttgaattac ttcggtaaac agatgacaga aatacttaat ggaccaacag aagacatcaa
     2881 gtcaaaatta atagagttat tagtcactga gcttgctgtt aatgattatg gtgtagataa
     2941 agagattatc aaacatctga ttgctactta tctattgtac gatatcgctt ttgcaaaaaa
     3001 cagtgtgttg atgtcgatgc ttggaggtac agatgatggg acaacgccta cagaaaacac
     3061 aggcttctat ccaaatacaa aacaacttca tcaaactgta ctaaacaaac aagatggcaa
     3121 aatgatggat gtatatgcat attatgtcga tcaaggttca gataaaacag tcatgatcca
     3181 tggcggattt cgagggaatt ggaataacgg gatcgttaca gaagaataca atgattttta
     3241 taaggccggg tataatttat tatttgttga ttccagagcg actggtaata gcggaggcga
     3301 ttatgtgacc tatggacaat atgagtcaga tgacgtcctt tactggataa atcaagaagt
     3361 tcgagagcgg ccatctcaaa agattttact ttatggtggt tcgatgggag cagctacgat
     3421 gatgtctgta ttagcgaaag atattcctgt caatgtcaaa ggaattattg aaaattgcgg
     3481 atttgcatca attgatgaac aactacgttt tacgtacagc cagacagtag cccccgcact
     3541 accggatgcc ataaaaaatc agttagacat cattggggat caagagcatg aagatttatt
     3601 tatgggcctt ttgaaacaat actattttga tcaggagatg catttggata caaaagctgc
     3661 tctacctacg attggaatgt cagattcatt gcctaaattg attatccatg gaacagcaga
     3721 tgatgtagta cctgtgtcta acgcgcaaaa attatatgag ttatcaggag ggtataaaga
     3781 tcttttatta gtagaaggag cagggcatgg aaaggcacag gaagttgacc atgctgccta
     3841 cacgaaacat gtaacggact ttttgaaagt agtgttccat gatcagataa acgtaaagta
     3901 tgtggatgaa aacaataaaa gtctactcaa agaccaggac gaaatccagc tttacggagc
     3961 ttatggagaa aattatatga cagagcaaaa gacattcgaa ggatacgaat tagcaaatgt
     4021 tgaaggaccg actgaaggga tttttaatga gacgatacca actattattt tctattacaa
     4081 aaaaatccca gtagctccac caaaaaagca agatccgaca gagaatgcag acaataaggg
     4141 aaagaatgaa cctcgagagg atcaatcaag tgatgatacg caaccgattc agatgacagt
     4201 gaaagtacaa aaaaagacag atgcaaaaaa aagaatgttg ccgaaaactg gagaaaagaa
     4261 acattcgctt ctcatttatt caggtggatt tttatctgtt agcacgatta tcacatggat
     4321 ttggcgaaga aagagagggt aaatgaatga aatggttcaa gcgaatagtg attgcagtac
     4381 cagttatcat cgtagttatt ttagtcactg tatttttgat taatcaaatc acaccaaagc
     4441 cgattagctt gcttgttaga aaacaatttg acacaagtga aaaagaacaa gtatatgccc
     4501 gaccagatga tttccagcaa aaaatcgaac aaatcgatat aaaaaaagaa atatcctatc
     4561 cttcaaagta caaaaataat aatatggata tctacacacc gaaagtggaa aagaaaaaat
     4621 taccgattct tttctggatg catggaggcg catatgtagg aggggataaa aatgattgtc
     4681 gtgattattt ggaatatcta tgttcagaca cgcagcaaat catcgtaaat atagattatg
     4741 aacgatcacc ggaagcaaaa catcctacac cagttatcca attgaacgaa gccattcaag
     4801 cagctaaaaa agagataaaa gatcaagcag attggtcgaa tatttcgatt ggtggaaatt
     4861 cggcaggtgc acaaattagt ggagaatatt tgttagcact tcaaaatgaa gaaattaaaa
     4921 aagctgatca tcttgatccg actttggaga acaagcagat tacgaagttt atttctttgt
     4981 ctggtctgtt ggatccttct gcacttactc aagtaagtga taaaatctct agctttcttt
     5041 atgaaaaatg tggatgggct tactttgata ataaggactt tgaacaatca gagaaggtca
     5101 aaagtttagc cttggtcaga catgcggatc gttggacgca aaacacattc ttaacggatg
     5161 gaaatacaaa tacgtttacg aaacaaatgg aagaagtaac ttctgtattt gagaaagcgg
     5221 gggtgaaagt cacaaaagta gattatgcgg agaaaaatgc tgttttgaac catgaatatc
     5281 agtttgattt cagcaataaa caagcgcaag aaacatatca aaagttagta atgtttttca
     5341 aggagtaaag tcttatgatg caaaagaggt tgtaatctgt cacttagctc caagtcacaa
     5401 ggagcaattt caataaacaa gcttttcatg gtttatgaat attgtaactt gtgactgagg
     5461 agttagagat ggcaaggggc accttttatt cgaaatttgg gaatatcaca cgattattgt
     5521 cacgatctct tttacgtaaa ttttataagt cagatttgtt gttttgatcg tttaaaaaaa
     5581 gcaacaaatc cgaagcctgc ggaggcaaga agggacaaaa agccgagtgg taagcaaaag
     5641 aatgctggtt ccactaatat tccgttgtta ttgatataag gctgggaaca aagatgaata
     5701 gcaaataaaa tcaatgcact agcgaaaaaa agcaaagaaa tatggatagc tttcataagc
     5761 agcacctcct tttttataat tgtatgataa atagattaag aatctataaa tgtgacaagt
     5821 ttgttaggat gtgaataaaa tgatcatcta gcgaacttga aacaaagatg gctaatgata
     5881 agcaattaat gattcagcaa tcagataggt acaaagttta ccatcatacg tctagaccaa
     5941 taatagaaaa tatgggaata taatagaaaa gatttaggat acccccaaaa gaatttcccc
     6001 aaaaattcga tgtatcctaa acttttttat tttacataac aatcatatca actgtgaaat
     6061 aaaattacaa atgataacct aaaatgaggc caaaagccag tgttatcaag gaaatactta
     6121 attcaccaaa atttttttta attttttaga aaataagaaa caaaaataat aaaaaaaaga
     6181 aaagataaat tttttactac tgtatactaa ctttgttatc gcatctaaca agtaactcga
     6241 ataataccaa tacaaccttc tccaaagatg cgatgacata cgttctaacc caccgactat
     6301 tgatgttctg taccaccctt gcagaaccaa aaaagccact accaaatatt tggtagtggc
     6361 tttttttgtt ttatggattt ttcgcttttt ctgttatggt gctattcttt gatttcagaa
     6421 atagtatgtc gaactttagc gatagaacga cgtgattctt tttcgttgta aaacatcaaa
     6481 atcacatata gagagtccaa aatgcttagt tgagaaatac gggaagctaa agcttcagat
     6541 cggaattctg tttcttcaga aatagaaata aaaacgacat cgccaagttt agcaagggaa
     6601 gaattagctt ggctagtaat aacgattgtt tttgctccag tttttcttac ggtttcagca
     6661 atatggattg tttcttttga ttgccctgtg tgagaaatca aaacagcaca gtctttttcc
     6721 gtcaataaag aagcttccat caattgaata tggtaatccg tgctgtgaga agttgcgata
     6781 ggggaacgca aaaatttatg ataagcgtca gttgctacaa tctctgaacc gccaacaccg
     6841 aaaaaataga cacgtttaga ttcattgata atagtagccg ctttttttag atccttttgt
     6901 tgtaacaact tttttgtgtc atttaaagtt gaaatactag actcgaacac cttttgcgcc
     6961 atagtgagct cagtatctgt tttctgaata ttttcgtgga tagaaatagc agatagatca
     7021 ttttcttgga tcaacatggc cattttaaaa tctttaaatc cattataacc aagttttttt
     7081 gtaaattgaa aaaaagtaga gtcagcaatg cccagttcac ttgctaaatt gctaatagga
     7141 ctatgactta ctttgcttgg attttctaaa atataatcgg cgatagcctg ttcttttggg
     7201 ctgaagtcgt tgtacattgt tttgatttgt agtcttatgg agacgctcat gagaattcct
     7261 cattttcata ataagattat cattttttcc gttctcatta atgttaaaga tacaaggaga
     7321 gtaggaaaaa atcaaacaaa aaatattttt gtaaaatcta tttacaaaaa aatttttttg
     7381 tataatgagg gtataagaaa tggaggcgct atcatggaaa ttggtgtaat tggtttggga
     7441 aaaatgggat tgaatttagc tttaaactta aaagagcacc atcacaaagt acaaggactc
     7501 gacatttcaa gtacagctgt tagtcaggca agagaaaatg acattcctgc ttatcaagag
     7561 atctctgaat ttctagctac ttttgagcag aaaaaaatca tctggttaat gcttcctgct
     7621 ggagagataa ccgaaaacat gttaagaaaa ctttttccaa aactcggacc aggagacatt
     7681 attattgaag gcggaaactc ttattataaa gattcaatcc gtcgagcaga agagtttcag
     7741 aaaaatgaaa tcggttactt ggattgtggg acttctggag gaatcgaagg agcacgacat
     7801 caagcctgct taatgattgg aggagataaa gaaacttttc tatccgtgga acaattattc
     7861 aaagatgtag caattgaaag aggatatctg tatgctggtg catccggaag cggacacttc
     7921 ttgaaaatga tccataatgg aatcgaatat ggaatgatgc aagcaatcgg tgaaggattc
     7981 caacttgtac aaaaaagtga gtatgctttt gacttggaac aagtcgcacg tgtttggaat
     8041 catggttcgg tgattcgcgg ctggctgatg gaaatcatgg aagatcagtt aaacgaacat
     8101 cccaatttaa gtaattatcg gggaatcgtt tctgcatcag gtgaagcaaa atggacggtt
     8161 gaaacagcgc tagaaatggc tgttccagtt ccagtaattg caactagttt gttcatgagg
     8221 aatctttcac aggaagaaga tagtttttca gcaaaagtag tttcagcact aagaaatggg
     8281 tttggcggac atgaaatagt tgaaaagggt gagtaaaatg ccagattatc atatagcaat
     8341 cgttaattcc agcagtttcg gaagagtctt tccacaccac ttggaaagat taaagtcagt
     8401 tggagacgtt catttcttta ctgttgatca agagattcca ggaaaagaac tagcggagtt
     8461 acttaaagga ttcaatatca ttattgcaag tgttacgcca ttttttcgac gagatttttt
     8521 tgaaaacaaa gatgaactgc tattgatttc ccgacatggt attggttaca acaatattga
     8581 tttaaaagca gcaaaagaac accagacaat cgtatctatc atacctgctt tagttgaacg
     8641 aaatgctgta gcagaaaata atataactaa tttgttgtcc atgcttcgac gcacgacaca
     8701 agcacaagca agagtcaagg acgatcgatg ggaagatcgg gcggagttcg tcggtaggac
     8761 gctgtttaac aaaacagttg gtgtgattgg tgcaggaaat accggaagcg gagtaatcga
     8821 aattttgcgt aacggttttc gctgtgatgt tctagcttat gatccttaca aatcagattt
     8881 atacatcgaa agctttggtg caaaaaaagt agaacttcaa gaattactgg aacaatcaga
     8941 tatcatttgt ttatgtgcga atttgacaga agagaactat cacatgattt ctgataaaga
     9001 agtcgatcag atgaaagaaa atgtttatct atctaatagt gcaagaggcg ctctagttga
     9061 tgaagaagcg atgattaatg gtctgaaaag cggaaagatc gcaggatatg ccacagatgt
     9121 tttagaagaa gaaccgggac gtcaagatca tccgtattta gcgtttgaac aagtagtgat
     9181 tacgccacat atatcggcct acacaaggga atgtttagaa gcaatgggtg aaaaatgtgt
     9241 cagagatgtc gaacagatca gtcagcacct tcttcctgaa cgttctgtcc agacaaagag
     9301 caactatata aaaggatgat gaagaaaatg gtgaacggtt taaaagtgaa aacaggccct
     9361 caattttatt tatatgaaga aggtggtatt tcaaaagttt ctgatctctt gaaatcatac
     9421 ggcgcgaaaa gagtactggt cacccacgga acggtatcat gggaaaaggc gctacctaaa
     9481 ttagtatttt tgaatgacga aacgattcaa tttttttatc atcgttattc tggagaatgt
     9541 agttatgctg aagccagacg aatagctaca atcatcaaaa aaatgaaatc gatttcgtag
     9601 ttggcgttgg cggaggaaaa ctgaccgatc tagtgggtta tagtgccaat cttgcaagaa
     9661 tcgatttcgg agtcatccct actttagcta gtaattgtgc tccatggaca cctcttgctg
     9721 tcatgtataa ggagaatgga caagcagaag ggaaaacaga acacttttac agacaagctg
     9781 ctttttttat aacagatccc caattagtga ttgattcccc tgttccatat ttcattgccg
     9841 ggttagcaga tacactagca aaatggtatg aatctgagct catcttagaa caagattttt
     9901 tgcaagatga atcatttttg aagctagcac aagatactgc gaagatctgc aaagaagaga
     9961 ttttgaatca ttctgctaaa gcaatccaag atatggcaca aagaaaactg acatcggagt
    10021 tcaaacagct ttcagagatt atctttgctg ttgctggttt agttggagga ttaggagata
    10081 aatacgcaag aaatgtggca gcacatgcta tgcatgacgc tatcagtaaa tatattccgg
    10141 aaagccatcg ttttctacat ggagaaaaag tagcgtatgg gattttctat caactggcac
    10201 tagaaggtcg ctgggaaaaa atagacgagt tatatccttt gtatcgggaa ttagaactgc
    10261 cggtttctct agaacaaatg ggattatttc cgaaagataa acagataata gataaaatgg
    10321 ctgaatttat tgattcgaaa gagaaagtgc atttgattcc tatagaggta acacagaaat
    10381 cactgataca gtcaatgatg gaactggaag gttatatcag aaagatctga aggaggagga
    10441 cacatggaaa cattaagtgt agtacaaagt ttgttgattg ctttatgggt agccgcggtt
    10501 atgtcccgct ggttaggtgg aggagccacg ttgaccttac gtttttctcc tttgatgact
    10561 ggattagtcg tcgggttgat catgggggaa gttgcacaag cgatgattgt tacagccgct
    10621 ttgcaaatga tttatatggg ggttttttct ccaggaggat caatgccagc tgaaccatct
    10681 attgcagcag ctatcgcagt accagtcgca ttattagggg atctgtcgcc agaagcagcg
    10741 attgccgttg cggtgccagt cggattgctc ggaagttact tgtatcagtt tcgttttttc
    10801 atcaatactt ttttaggtaa atatacagac aaagctgtag aagatttgaa tacaaaagct
    10861 atggcccgat caattattct ttatccaacg gttgcctcat ttttattatt cgttccgctt
    10921 gttttcattg ctttatattt aggggcgcca ttgatcgctg atgtaatcaa agctttagag
    10981 ggaacagtcg ttatccacat tcttgaagta gtaggtggag ggttagcggc aatcggtatc
    11041 gcgacaactg tatacgttat cggtcgaaaa gattatctgg tctttttctt ccttgcttat
    11101 tttatgagtg ttgttttgaa atctttagat attacgatgg ttacttatgc catttttggg
    11161 attattatcg ctttgatctt tgtccagtcg caaaaaggga aacaagtaca gcctgccaca
    11221 agcaataaca attcagcatc ttttgatgac gaagatgact atgatgacgg attttaattt
    11281 aggaggaatg ggaaatggct gaaataatgg ataatcaaac aggtgctcct gaagaaatca
    11341 cgaaaaaaga tgtaacaaaa gcctatctga gatggcattt tgcaaacgaa attcctcact
    11401 cttttgaacg ttacttggcg ccatcactac tatacggaat gatgccaatc ttgaaaaaac
    11461 tttataaaga tgaagactct ttaaaagcag catataaacg gcagttactg ttcttcaaca
    11521 ctcaattaag ctggggtggc ggtgtgatta ctggcctaat gtcttctatg gaacagcaaa
    11581 gagcagaaga agagtatgaa gaaaaagaga tcttgatgca ggatgacttg atgtataaca
    11641 cgaaagcggg attgatgggg gcactagcag gaatcggaga tgcaatcgat tcaggaaccg
    11701 tccaatatat ttttatcgca atcgcggtac cttgggcaca acagggaagt cctttaggcg
    11761 cactttttcc ttttatctgc tttgggttgt atcaggtttt actaggggta tttttcgcaa
    11821 gacaagcttt cactatgggc cgtaatgcta caggcctaaa gcaaaacact ggtgttcaga
    11881 cagcgattga aatgctatca gttttagggt tgtttatgat gggggtgctt gcgggaaact
    11941 atgtaaaagt agaatcttca ttgaaatttg cgatatccgg acgtgagttt gttatccagg
    12001 aaattcttga tcaaatcgtg ccagggatat tacctttagc tgtcgtaatg ggtgtgtact
    12061 tgttctatac gaaaaaagga ttgaaagtca cacaagcatt attatggctg actggaatat
    12121 taattgtgct agcaggaatc ggtattttat aaaaagaagg gaacgtgaat aagatgggtg
    12181 tagtcaattt agcgcgtgtg gatgagcgtt tgatccatgg gcaagtaatg gtaacgttat
    12241 ctcaaaaaag tggagtgaat tccatttttg ttgtagacga agttgtagca aaagataaat
    12301 ttatgagaga tctttacaaa aatgcaggaa gccgtacagg acaaaaaaca attgtcatta
    12361 caccagagaa agctaaattc tattgggatg aatatcaatt taaagaatac aattgtattt
    12421 tgatcgcaaa aacagtccaa acgatttacg acttagtaaa acacggaatc cccatggaag
    12481 aattgaatat tggagggatt gctcaaaaaa atcctgatac agatctttta gtgacgaaat
    12541 ctgtttattt aaataaatca gatgctgaaa aattgaaaga aatgaatgaa caatatggcg
    12601 taaagaatat ttattttcaa gccactccat ctgcacctaa aacaggatta aaagatgtat
    12661 tgaacaagtt tgatttgtca taaacaaatg cttagaaaaa agataaagag ggcgaaaaaa
    12721 tgaagaagaa cgagctgttt cgtgattggg aatttcgtta tcgctatatt taccgtaagc
    12781 gccgtacaaa aaagagtaaa cagcgttttc tttcagcatt agtatcagat atttacagta
    12841 tgagaacaga tgtgacagtc attgcttacg atacgctagc ctaccgatca aaaaatattt
    12901 atgttggaga tatcgaaaaa gcagagaaag ttatctgtac ttattacgat acacccgttc
    12961 atgcattagg aagttatttc atgtttgatt ggaaagacca aaggaaaaaa acaatctact
    13021 ctatattatt gtccttcatt ttgttgttca gtttaggttg gtggggaatg atgatatata
    13081 acaaaaatcc tcatcacgta ttcgatctgc tatccgtata aacaggtatc actgtactag
    13141 catttgggag ctactttttc ttattgggaa aagcagctcg cggatggcct agcagacaaa
    13201 cttttattcg aaatacttca tccatattaa cgatgctaga aatgatccgt acgattgatg
    13261 atccgagtgt agcgtatgcg tttgttgatg aaggctgtta tggagaaaag ggcttagaca
    13321 gcgtgagatc gggtatgaaa aaagaggcta ttctttttta tctagactct gttggggcag
    13381 atacaccgct acagttttcg ggaaattatt tttcaaataa agaacagtgg ctgaaacaag
    13441 ttgataagtt gaaagaaaaa aacgtcaatt atatttttag tgcaagaaaa aagcaagcgc
    13501 agttcttcta tttgaccaaa acagatttac gagggaaaac atttaattgg caaaatgcca
    13561 atcagattat tgctcttttt cgataagtaa aaagtgcaaa acaaggagtg agaagactaa
    13621 tgttggaaat catgatcgtt acacatggta agttgagcga aggactgaaa gatgcggcgg
    13681 aagtaattgt cgggacatcg agccatattg aaacaatgtg cttagagtct ggggaggaca
    13741 tccaaacatt cggagagaag gtcaaagaaa aattaactat gatcaacaca gcagaaccca
    13801 tgctagtatt tgttgatcta attagcgcaa gtccgtacaa tcaagcgctc ttggcagtca
    13861 atgaactgac tgaagaaaag aaagcagcca cttatgtcat agggggcgtg aatctgccaa
    13921 tgttgttaga agcaatcaat catcagatgc ttcacacacc gatagaagaa gcggtggaac
    13981 aaattgtcaa acaaggaaaa gataatttaa ctgtttggca tgcttcagcg gaagaaaaaa
    14041 aattagatga ggacgatttt tagaaggaag gctgaaaaga tgaagtgggg atttagatgg
    14101 tatggtgaaa aaggggattc gatcccatta aaatatgttc gccaaatacc gggaataaat
    14161 gggataattg gaactttgct gactaaaatg cctggagatg tttgggagat ttcagaaatt
    14221 gaggagttga aagcatctgt tgagagccaa ggactggaac tgcttggaat tgaaagtgta
    14281 gcgatccacg atgcaatcaa ggcagggaca gatgaacgag attactatat tgataactat
    14341 atacgaacaa tcaaaaactt gtctcgatgt ggggttcgat tgatttgtta tagcttcaaa
    14401 ccaatttttg gatgggcgaa aactgatttg tttaatgaaa acaacgatgg aagctattct
    14461 cttttatacg atcagaaaat cgtcgatcag atggagccag gagaaatgta tcaattgata
    14521 cacagccagt caaagggatt caaattgccg ggttgggaag aagaacggtt gaaaaaattc
    14581 gctcagctgg aaaaactgta tgaaggaatg accgaagaag aattatttga aaatctgact
    14641 tattttttag aaaaagtgat tccagtgtgc gaaaaagcag atgtaaaaat ggctatccat
    14701 ccagatgatc cgccatggga actttttggt tttcctagag tgacgaaaaa tcttggcgac
    14761 ttgaagaaaa ttctaaatgc agtagattct ccatataatg gactaacgct gtgcacagga
    14821 tctttagggg caaatccgga aaacgattta gttgaaatca tccatgaggt aggttcgcgc
    14881 atccattttg tccacttcag aaacgtggag tttttaggag aaagaaagtt caaagaaacg
    14941 gcacatccct ctacagaagg ctcccttgat atgtatcata ttatgaaagc gttggtgcaa
    15001 gaagggttcg acggtcctat acgccctgat catggtagaa ccgtgtgggg tgaagtggct
    15061 atgcctggat atggcctgta tgatcgtgca atgggactca cttacatgca aggtctatat
    15121 gaggcgataa gtaaggaaaa accactggga gagcattatg aataaaatca agctcttcat
    15181 ctttgatatg gatggccttt tattagaaac tggacgttta gcttatcgtg cttatgtaaa
    15241 atcagcacaa aagtatgatt atgaaatgcg taaggaagtc tattatttac taactggcca
    15301 aacagaaatg gcgataagag aacaaatggg cattttgtac ggagaagatg ttccatatat
    15361 caaatggcga gaagcaatca atcaatataa agaaaaaatc gtgaaagaag ataagagagt
    15421 atatacgaaa aaaggagcag aagaaatttt atcttttgca aaagaaagag gaatccatac
    15481 aatcgtggct tcgtctaata cacgggtaaa agtagaaatg tatttaaaaa tggaaaatct
    15541 atatgccttg tttgatcaaa tcatttcagg ggatgatgta aaaaaaggaa agccggaacc
    15601 agagatcttt ttgaaagctt gttcaaaaat gaatattcct ccaagtgaag cacttgtatt
    15661 tgaagactcg attgcaggga tcgaagcagc aaggcgagcg ggcattttgt cttttctcat
    15721 caaagatcat ttgatagggt tgcccgatca taatgggaag cataagttga aagtagatgt
    15781 aagtttatat gaatccaata aagcagacta tgaattttat gacctgcatc aagcaaaacg
    15841 atttttagaa agtaaagggt tgtatctcta ataaagagga ctattttcta gtcctcttta
    15901 tttattgtcc tgcacgactg agggaacaga tgaaaaagaa gtaatttttt attttgaagt
    15961 atctacctta tttgtagtga acaatttaga acaaacacaa aacgcatcta ctgttatcta
    16021 ccagtagatg cgtttaaaga gaagaacgag aatcgtccat atgagggttt ccactcctta
    16081 tgcagatttt tttaattctt gcactgcttg ttcgatcgta acacctgtcg ctacatgatt
    16141 ggtattttct ttgtcaaaaa cgacaaatag attcaaatct gtatccaagg ttactcgata
    16201 ttgtatatct ttgttataga atgtcatagc cattctcctt tctttaaata cttgctgaaa
    16261 ggcaacgaac gcgttgataa cgaaattcca tttataagta taccaccaat tgaaaggttt
    16321 tcatagttaa gaaattttaa agtcaaaagt tgcagataaa agcaaataaa caagaaaaca
    16381 aaaagtgaat caatcgcggt tattcaactt tttcttcgat tcgaagacaa tagaagacaa
    16441 tgaattttat atttcattgc attatccttg tgttattaga aaaagcatga tatattaaat
    16501 gagtaatcta ttttgaaacg taatttgagc gatatatcta catagataaa actccttttg
    16561 cctatcaaat atttattgca acgaaaaaac gtacattgat acgtatcagg aggaatttat
    16621 ttatgaataa cggtacagta aaatggttca acgcagacaa aggttttggc tttatcactg
    16681 gtgaagatgg aaatgatgta tttgcacatt tttcagctat ccaaggtgac ggcttcaaaa
    16741 ctttagaaga aggccaagca gtgtcttacg atgttgaaga tggtcaacgt ggccctcaag
    16801 caacaaacat tgttaaagct taataaaaat tgacttttaa ccgtcttttc gaaagaaaag
    16861 ggctctttgt caaataggac tgatagagtg agataagaac atctaaggta gaattccttt
    16921 tctactttag atgttttttg attcataggt aatcagatta tttagtaaga aaattctggt
    16981 cttcattttc cgaaagcttc gaaagccata agcgactcgt ttcaatgctt tgatgtgggt
    17041 atgcagattt tctattttgg catttgaata aggcaattcg agggctaaac gaatgccgga
    17101 ttcatagttt ataaaagctt tttttataga atgcagttct tttggaacgg aattgggcat
    17161 cgttcttaca agtttaaaga aagtgtccgc ctctttgtcg tgaaacgctt gaagaatttc
    17221 ttgatagtaa aaataggcga ttcgtaattc gtcagagatc gaaagcagac gatccaccac
    17281 ttcactttcg gtgagatacg cccaaccaaa attgcgccat ttggtccatt tgtcataaac
    17341 aagcttgtcc tgtcgtttga tcagaagccg agacaacgcc tttaattgtc gataatattt
    17401 ggcttgtgtt ggatcgtttc gatcaaagct tttcatgatt cgtacgcgaa tatcctcaaa
    17461 tttacgaatg agatgtttga caatgtgaaa acgatccaca acgagttttg cctttggaaa
    17521 acattccttt atcagagagg cataagaaac attcatgtcc gtcacgatga cctttacctt
    17581 caatcgtgcg cgtcgttcaa attgcataaa ataagaaacg agagcctttt tcttccgtga
    17641 aggaagaata tccagtaatt ttccagtatc gccatccata caagaaaagg agtagggacc
    17701 gacttgattt ttcagtgaac gaaattcatc cattaataaa caacgcggta accaatttct
    17761 ttttactttt gtcatgggga tcagagaagc gagtgtccga gaaacggtcg accaagaaac
    17821 gccgcaatgc ttagcaattg tcgtcattgc ttgtttttca gttagctctt ctaaaatttt
    17881 atagcgcact tgtttagcga taaagcagtt tctatcgaca atgtaggtat tcgccgtaaa
    17941 ataggtgtgg cagccacgac agtggtagcg ttgtttcgct aaagcaagat aagtcagttg
    18001 gttcgcacat ttattaagtt gaatgagcga ttttttcttt ccgttcttaa cgatcatgcc
    18061 ttcatgattc atctgtttac acaaaggaca gcgacgaatt tctttactta acgtcgcaga
    18121 aacaacaaga ctcttgatat tcttaatgct cttcgattcc acatttgtga tgaaaatgtt
    18181 tttatctttg attccaagta gtagtttgat atgatgaaga tcaggcatac agaacgctcc
    18241 tttgattgtg ttgtggtgat tcaattctaa cggagcttct gtatgttttg ttttttcatg
    18301 ctcaaaaggg accgatagaa tgattttcta tcagtcctag aaattataga gccaagaaaa
    18361 gacggttttt ttgtacg
//
LOCUS       DACDIG010000054        18018 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.054, whole genome shotgun sequence.
ACCESSION   DACDIG010000054 DACDIG010000000
VERSION     DACDIG010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.054"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..187
                     /locus_tag="IV247_001976"
     CDS             <1..187
                     /locus_tag="IV247_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778038.1"
                     /translation="VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAA
                     IITQLFLVFGEKKRSKK"
     gene            complement(278..643)
                     /locus_tag="IV247_001977"
     CDS             complement(278..643)
                     /locus_tag="IV247_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778039.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            851..1333
                     /locus_tag="IV247_001978"
     CDS             851..1333
                     /locus_tag="IV247_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778040.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1530..2420
                     /gene="pstS"
                     /locus_tag="IV247_001979"
     CDS             1530..2420
                     /gene="pstS"
                     /locus_tag="IV247_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAP8778041.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            complement(2707..3981)
                     /locus_tag="IV247_001980"
     CDS             complement(2707..3981)
                     /locus_tag="IV247_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP8778042.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTWEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHCYQSGISNAFKVSHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            4254..6725
                     /locus_tag="IV247_001981"
     CDS             4254..6725
                     /locus_tag="IV247_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP8778043.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HANQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(6828..8564)
                     /locus_tag="IV247_001982"
     CDS             complement(6828..8564)
                     /locus_tag="IV247_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAP8778044.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(8561..9265)
                     /locus_tag="IV247_001983"
     CDS             complement(8561..9265)
                     /locus_tag="IV247_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8778045.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            9396..9899
                     /locus_tag="IV247_001984"
     CDS             9396..9899
                     /locus_tag="IV247_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAP8778046.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            9859..10197
                     /locus_tag="IV247_001985"
     CDS             9859..10197
                     /locus_tag="IV247_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAP8778047.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            10218..11636
                     /gene="ffh"
                     /locus_tag="IV247_001986"
     CDS             10218..11636
                     /gene="ffh"
                     /locus_tag="IV247_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAP8778048.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            11748..12326
                     /locus_tag="IV247_001987"
     CDS             11748..12326
                     /locus_tag="IV247_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAP8778049.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            12330..12851
                     /locus_tag="IV247_001988"
     CDS             12330..12851
                     /locus_tag="IV247_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8778050.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(12930..13484)
                     /locus_tag="IV247_001989"
     CDS             complement(12930..13484)
                     /locus_tag="IV247_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP8778051.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            13737..14012
                     /gene="rpsP"
                     /locus_tag="IV247_001990"
     CDS             13737..14012
                     /gene="rpsP"
                     /locus_tag="IV247_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAP8778052.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            14024..14275
                     /locus_tag="IV247_001991"
     CDS             14024..14275
                     /locus_tag="IV247_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP8778053.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            14400..15191
                     /locus_tag="IV247_001992"
     CDS             14400..15191
                     /locus_tag="IV247_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778054.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            15361..15882
                     /gene="rimM"
                     /locus_tag="IV247_001993"
     CDS             15361..15882
                     /gene="rimM"
                     /locus_tag="IV247_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAP8778055.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            15872..16633
                     /gene="trmD"
                     /locus_tag="IV247_001994"
     CDS             15872..16633
                     /gene="trmD"
                     /locus_tag="IV247_001994"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAP8778056.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            16769..17116
                     /gene="rplS"
                     /locus_tag="IV247_001995"
     CDS             16769..17116
                     /gene="rplS"
                     /locus_tag="IV247_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAP8778057.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(17248..18009)
                     /locus_tag="IV247_001996"
     CDS             complement(17248..18009)
                     /locus_tag="IV247_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8778058.1"
                     /translation="MIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKNPIDEVVIDY
                     KRESKTIKIKDKFLEDDEYNRLVDFTTSHNKRYSLLFQWLYLTGMRSGEAIALSKDDV
                     HITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAIYNELLELNP
                     NGQFLFQTTKGTPFQLTAINTYLRSHKADMKIDKKLSSHIFRHTHISKLAELGTPLYA
                     IQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
BASE COUNT         5897 a         3232 c         3642 g         5247 t
ORIGIN      
        1 ggtatactca caaactattt tttatggagg aattgtttta attagtttac tttcttcaac
       61 actttattct ttgaatatta tagatgtttc cacctcaaat tcaatatcta ttattggaat
      121 cattattgct gcaattatta cacaactgtt tttagtgttt ggagagaaga aaagaagtaa
      181 aaaatagaaa aactagtgat agttttggtg atataagaaa aacatatatg tataaaaaga
      241 agaaagtagc ttctctttgt acatatatgg tttgttttta tggatgccga gcttttttat
      301 aagataaggc taccgcccat aacgacccaa ggatttgtgg aactaagtaa atacctgcaa
      361 gaagcaagat cagctgggtc ggttttaaag gatgatagaa gtaacgccat tcagtaaaat
      421 tggcgcaaaa taaataggaa gtgacagtag atataatatt ggcaatgaat aaagatataa
      481 tacgcctgtt cctttgaaaa taatatccag caaaaagaga taaaaatatc aatgtgatga
      541 acccgcttac ggaatgataa cgaaaatcaa gcagtaaggc gaaatgcaca taaggaacaa
      601 aagataaaag caacggaagg tttttccagc aagtttttat catctttcat caccacctaa
      661 caagtgttat tattttgtta gttttattgt aaattgaatc ctgcaataat caattacttt
      721 ttcattattt ctagattaaa tgaataaact ctcatgccat tatctctaaa aaaaaagttt
      781 ttccccctaa tttttttaga tagaaaattg tatagtatga tgagagaaaa agtagtttgg
      841 gggaacgtta atgagaagaa aagcagtaat cgatcggctt tatttatttt tttggttttt
      901 tcctatcgtt ttgctacctt ttacatgggt aaatgctgtt gtacaaggtg cggagatatt
      961 aggcctgatc caaggaagcg gatttgtggt ttctaggaat tttccagctt tgattgtttt
     1021 ctatttagtg ataagtgtag gtctcttatt accaaaatct ctgatcaatc actacaattg
     1081 gcagctgctt tacttttctt gcttattagt atttacatct atgttttctt tgctgcctcg
     1141 cttgattatc ggccctaatg gtttgagcga gagtggattg aaggatttag cttattcctc
     1201 catgtatttc ttgacgatca aaccatggtt ctatttagta attgcctcat ttattcttgc
     1261 aacatttttt tacttctgga cagaaatcag gataaaaagt gaacgatctg tatctataaa
     1321 aaataactat tgatggtgga tcatcagtaa aaaaacatat gagctgcgta aaatgcatgt
     1381 ttctcatatg ctttttttat tattatgtaa agaatgcgaa aaacgtttac atgattttta
     1441 cataacttaa ctatattttt acagcaacca tacgagggat ttacagtcga ctgataaact
     1501 ttagttgtaa ataagaaagg agcaataaaa tgaaaaaagt attgtttact ctaggtatga
     1561 tgggaattct tttagccgga tgcggctcag gatcaggaaa tacagatggc tctgccacac
     1621 aaaataattc aagtgactca aatgaacaag taaaaatcgt tgcggtaggt tcgacagcat
     1681 tacagccgtt agtcgatgca gcacaagaaa gctttgtaca agaaaatcca aattatcaaa
     1741 tctctgtaca aggaggagga agcggaactg gtttgagcca agtcgaagct ggagcagtta
     1801 ctatcggaaa ctctgatgta tttgcagaag aaagagacgg agttgatgct tcaaaattag
     1861 tggatcataa agtagcagtg gtcgggatgg caccaatcgt caataaagat acagatgtca
     1921 aagatatcac caaacaggaa ttgattgata tctttacagg aaaaatcact aactggaagg
     1981 aagttggcgg gaaagatcaa aaaatcaacg tcgtgaaccg tgcaaacggc agcggtaccc
     2041 gcgcgacatt cgaaaaatgg ggactggatg gtgctacccc tgtgcagtcc caagaacaag
     2101 attcatcagg aacagttcgg cagctggtaa gtcaaacacc aggagcaatc agctatttag
     2161 ctttctcata tctcgatgat tccacacaag cactaagtat cgacggtgta gaaccaaaag
     2221 aagagaatgt agcagataac agctggggaa tctggtcata tgaacatatg tacacgaacg
     2281 gaaaaccttc ccctgaagtc caaaaattct tagactacat gatgacagaa gaaatccaag
     2341 aaggacctgt caaagaattg ggctatttac caatcacaat gatggaagta gaacgtgatc
     2401 acgaaggaaa tatcaaataa ctagcagata aacatgccac ggtagtcgtt actattaagg
     2461 aaaaatcaac tatgtttccc cacatataca tttttccttt aattgtgaaa aatagttcct
     2521 catattttgc cgttttttga gctaataccg agggtcagct gattgaacac cgtttattcg
     2581 aaaaaaagag ctacgacata gaactgtcgt aacttttttt ggctcttcgt caaatcgtgt
     2641 tggtgaagac aaatttgaat aaaacttagt ccgatatgcg gcgcgttctg tgcgccgcat
     2701 atttttttag ttttcaaaga tcagaccttg ttgtaaatag atccgtgccc gaaagttata
     2761 aaagttgcga taaccaaacg caattcgttt aatcagcttg atcttgttat tcagcccctc
     2821 agtgacgccg tttgagtggg acaccttaaa ggcatttgaa atacccgatt ggtagcaatg
     2881 gaagatcgtg aatttctttt taaacgactg aggtaacgta gatgaaattg ttttaagggt
     2941 ttcttgaaag ccttccgtac ctcgtttaac gaaataatag cgtaataatt gacaaaaatg
     3001 atacgcagtt cgcaattctt cgttgtagct taacagctca tcaacgattt ctgtttgact
     3061 aattggtcgt ttaaacagtc gatgataatg ccgtttgagt ggctcccatg tcgtggaatc
     3121 tttgagtaag agtttccaat atcgcttgag ccgacggtac ttcttctgat tttcagagtg
     3181 tgaggttcga aaacgattca tgatctggac acgtaattga ttgaagctgc gattgatgtg
     3241 ttggacaata tgaaagcggt cagttacgat ctccgcattt ggaaagactg ttttaagcaa
     3301 ctgacaatag ctggcattca tgtccatcac taggtacttg acctttaaac gggcctttct
     3361 aggaaagcgc aggaagtatt gagttagttt gtaaagtctg cgatcttcaa ggatcgtaaa
     3421 tagctgattg gttgtcccat caacacaaat aaaactcatg gaaccagaaa cagaattcat
     3481 tgatttgaac tcatccatac acaacaccgt cggaaggaag ttcatacgtg gctggtaatg
     3541 cttcgttgct tcacgcaaga tccgtgtcac cgtcccatcc gagacgaagt acttcttagc
     3601 gattagttta cgtgattgat tttcgtacag ttccatgaga atcgcctgct tcagttcctt
     3661 tgttagttga tgatgttcat caactaagtc cgttttcgca gaaaaggtcc ttccacaatc
     3721 atggcagaga aaacgggtac gttttaattc taaaatagtt aacttttctt tgactttcaa
     3781 catctgagtg cgtgtcgtat aggaaccgtt gcggataata cgaaccgatc gacagtgttc
     3841 acaataggaa gaagagtcat ccaattttcc tttaataaac tgcgcttgaa cgcctttata
     3901 ttctttttca atcagccatt cttcatcaaa aaataaatgt ttatctgtta atccgagtaa
     3961 ttttctagta tgattatcca ttgagagact cctttggtag gatgacgttt tggtcgacta
     4021 aattttacca aaagtgagtc tctttttgag ttgaaacgaa gaaaaagtgt tagtagaaaa
     4081 tcgccttcga ttttccacca acacaaaata ttatagaacc cttttttttt gctatatgac
     4141 aggaatgaaa ggtgtttgca agcttaatgt gaggaacact ttccatatct gttctatact
     4201 ggataggaga agtttggaaa tatccagaaa acgaaatcaa tcgaggtagc cacatgaaga
     4261 ataaatggat aaatcgaagt aaaaaaaaga aagtaacaca aagatcatca gataaagtca
     4321 tccgtctttt tccatcgtat aaagaaggat taacggcaga tcaagtcaag gaacgaatag
     4381 aaaaaggtgc agcaaataat tcagtcgatc ccacattcaa aacaaatcag caaatcgtat
     4441 tggaaaacat atttacgtat ttcaatttga tctttcttat cttagccatt ttgctttgtt
     4501 tagtagaatc ctataagaac ttgacttttt taccagttat catagccaat acaggaatcg
     4561 ccatttttca ggaaattcga tccaaaaaga tcttggacga actaaatgtt ttaaatacag
     4621 caaaagtcaa tgtcatacga gacgggatag aacaaacaat agatatagaa gaccttgtaa
     4681 tagatgacat tgttatttta gagactggat atcaaattcc agcagatgca gaggtcgtgg
     4741 aagggaaact gcaagttaat gaggctttat tgacaggcga agcagatgaa ataaccaaag
     4801 aaataggtga tccattaatg tccggcagtt tcattgtttc cggaaaagct tacgcccgat
     4861 tagacaaagt tggagcagat tcgtatattt cccagctgac agtcaaagcc aagacaatgg
     4921 gagaaggaga acagtcggag atgattgctt ctcttaatca attgatcaaa tggattggga
     4981 tcatcattat tcctatcggt atcctgcttt tttctcaaag ttactttttt aatggaaaca
     5041 cgatcaaaga aagtgtcgtt gcaatggaag cagcattgat cggtatgata cctgaaggac
     5101 tgtacttgct gacgacgatt gcattagcat taagtgcgac taaactagca aagcaaagag
     5161 ttcttttaca caatatgaaa agcattgaga cattggcaag ggtaaatgtc ctttgtgtag
     5221 ataaaacagg aacgatcaca gaaaataaaa tgtctgttca aaaaatgatc gtctctaaaa
     5281 aacaagaaat aatcagtcag tcagatcaat tggaagcatg cattgctgac tatgcaaaag
     5341 cgatggcagg agacaatgca acaatggaag ctgttaaagc atattttaca aaaaccactg
     5401 atcaatccta tcaccatgtt attccttttt cttctgtcca aaaattcagc agtgtagcgt
     5461 tggcagacaa ggtgtatgtt ttaggagcgc ccgaaatggt tttgcgcgat caattgccgg
     5521 aatatgcgga agagtttgtt tctttcgctg aaaaaggcta tcgcgtgttg gtctttggtg
     5581 tgtacgatgg gattcttgaa gaaccagtat tgacagaagc agtcacacca ttaggctata
     5641 ttctgattgc taatcctatc agaaaagaag caagagccac atttgattac ttcaaaaaac
     5701 agcaagtagc catcaaagtg atttctggag ataatccgct caccgtatcc aatgtagctg
     5761 tccaagcagg gatttcacat gctaatcagt acgttgatgc atctaaatta tcagaagaag
     5821 aatatgaagc agctgtcgaa aaatatacag tttttggacg cgtcaagcca gaacaaaaga
     5881 aaatcttcgt tcaattgata aagcgtaaca atacagtagc tatgacaggt gatggcgtga
     5941 atgatattct agccatgaaa gaagcagact gcagtatagc gatggcttcc ggaaatgaag
     6001 ccgccatgca ggcctcacaa gtggtcttac ttgattctga cttttcacgc atgccagaag
     6061 ttgttgcaga aggacggcgc gttgtgaaca atattgagcg atcagccagc ttgtttttag
     6121 tgaagaatat tttctccttt ttgctctcac tgttttcagt gatctttgct ctgacctatc
     6181 ctttagaacc atcacaaatc acattgatta gtttgtttac tatcggactt ccatctttct
     6241 tgcttgctct agaagaaaac aaaaagcgaa tcagagggaa attcatcatg aatgtgatgg
     6301 aaaaagctgt gccaggtgga ctaacagata tgatcgtagt cggtgcgttg gtgatctgcg
     6361 gggtgacttt gaatctaaat aaaacagatg tttctacagc atctaccatg cttttgattg
     6421 cagtagggtt cctcgttctc tataaaatct gttcaccgct gaataaattc agaagtcaga
     6481 tcattctttt ctgtgctagc ggtaactttt tttcggtcat ctttttgcat aagctgtttt
     6541 caatcaccgg tatctcagct gtatcacttt tgctgttgag cattctattt ttctcggcag
     6601 attcgatttt taggcatttg accacattgg tagaatattt gtttgaacgt aaaaatgaaa
     6661 aagagcaaaa gccgtttagt cgcgtagcta aaaaatttct atcgatattt aaacgagaaa
     6721 attgacgaaa agtatctcaa aaaagagtaa aaaagaacaa ccactcgtgt atctttcgaa
     6781 gcaggatgag tcatccaact ttaaaacgca cgagcagttg ctctttttta cattttcgga
     6841 atggtaacgg tgaaagtcga acctgttcca agatgactgt ctacagtaat cgtaccatta
     6901 aggagttgca cgtagtcttt tacaatcgct aaccctaatc cggtcccgcc tgaatgtcgg
     6961 cttcgcgcct tgtctacgcg gtaaaaacgc tcaaagatcc gttcctgatc ctcaatgtcg
     7021 atacctatac caaagtcttg gacagaaaac gatagatcat ctgttgcctg atagcggata
     7081 ataatctcgc tgtcgctttt tgaatattgg acagcatttt caatcagatt tttgataatt
     7141 ggataaagga tatctgtttt agtagtaaat ggttcgttct ttccaatcaa tcgaatcgtc
     7201 aaccgtttct tttcgatgat tggctgataa ttttgaacga tttgctggaa ataaggttct
     7261 gtataaattg tctggatctc gtaagaattc tcgccatctt ttgacagctg gatgatttcc
     7321 cgaatcagtt tatctaaccg gatcgcatct ttttgcatga tctcaagaaa agaagtcaga
     7381 gtttgcgggt catcttttgc accatccagt aaagtctcgg taaaaccaat cagtgaagtc
     7441 acaggagttt ttaattcgtg agagacgttg ccaacgaaat ccttttgtag cttttctaaa
     7501 cgtcgcactt tcgttaagtc atacgccacc cctagaatct gtccggtacc ttctgaatta
     7561 gtaaagtagc gcaaactcaa gtctaatgtc tgccgacctt ctgtaagggt gatctcctga
     7621 tggatcagcg gatgctcagg agtcacttga tgaatcagct gaatgagttt tggctcttga
     7681 atgacttctg tgtaccgctg ttcaggttga tagcttacca cgtttaaatg tagctgcatc
     7741 tttggattaa gcagtagtaa acgagaatca gcatcatcaa tcaggaatac gccaatcatc
     7801 agttcgttca gtaacgtata taattgctct tctgtggtag tacacgcccg atacatctta
     7861 ctcatttgtt cgctcaacgc attaatcgtc tgatacaatt cttcccattg atcgggtgtt
     7921 tgcatgatta cttccgcctg attaggattt tgcaccattt tttttagtac gggcagcacg
     7981 gttttcaatg gctggttttt ttgatagatc agataataga tcattcctgc aagaacaagg
     8041 aagaaagaca gaaagaagaa aaagacccaa cggcgaaaac tctcagttct cggtaaaaaa
     8101 acgcttgtcg gttctgcgat acgtaaaatg gcttctaact ggccactttt tttgactggt
     8161 aatgcgacat ataaaagatc ctcattcaaa gtagtacttt tgcgcaaagc gctacctagg
     8221 ttcccaccat tcaatacggc tttgatttcg ggacgggagt ttcgctgttc attcaatgtt
     8281 tcattgtttg tgtcaaataa gatctttcca gtagcatcca ttaaagtaat ccgttcgttc
     8341 gactgatgga cgaattcttc caaagcagtt ttgttttgcg ctgcttggag gttttcgaca
     8401 tctaattgcc ggatcagcag ttgtgctttt tttgtcaggt agctttcttg ctgttccacg
     8461 atctgctgtt gaaaataatg ggaaatcaat tgccagccac caaaaaacaa actaactaaa
     8521 agaataagaa agaagagacg ttcataaatt tttttccatt tcatcgtttg ggttcctgaa
     8581 acttatagcc aaatcctcta atagtcacca agtactttgg gtgttttgga tctcgctcga
     8641 ttttttctcg caaatgacta acgtgtacat ctacgattcg gctttgtcct gcgaagtcaa
     8701 aattccaaat gcggtctagc aatgtgtctc ggtcgatcac acggtctttt cgtttcatga
     8761 aataaacaag taattcaaat tctttcggtg ttagttcaat cagatggtcg tctacggtca
     8821 cttgataatt ggtcaaatca gcagtgattt gtccaatcga tagatattca ggctcagctt
     8881 catcttttga ttgcttacga ggttcaattc gtctgaatat tgctttcata cgggctaata
     8941 cttcgcgagg actaaatggt ttggtcaaat agtcgtcagc acctatttct aggccaatga
     9001 tccgatccac ttggtcatct ttagccgtca gcatcaaaat cggtgtttca tttttttctt
     9061 gacgcagccg ctgagtgatc tccatgccat ccatcgttgg aagcatcaca tctaaaatga
     9121 tgaaatcaaa tgattgttct aatgctaatt ctaatccttc gcctccatct gtggcactgg
     9181 ttaccttgta gccttctttt tccaaattga acgtcaagag cgttacaata gaaggttcat
     9241 catcaacaac tagtattttt ttcattcaaa tctcctttaa ataggtttca ttctgtttat
     9301 attttagcat agaatcagta caaagggcta ttttacaaaa gaaataaatg gtataatagg
     9361 aaggcacttt gctgaataga aaggaagagc agtcaatgat tggaatcagt gcatgtttag
     9421 gcggtctggc ctgtcgctat gatggacaga aaaaagaagt accagaattg atggaactag
     9481 ttgaaactgg agaagctgtc atggtctgtc cagaagtgat cggcggatta ccaattccta
     9541 gagatcccgc tgaaatcatt ggtggaaatg gattcgatgt gtggaaagat caagcaaaag
     9601 tatggacgac aagcggcgaa gatgtaacgg aagcgtacaa aaatggcgcg atcaaagcct
     9661 atcaaaaatt gcaggaaaaa cacattgatc tagtcatcat gaaggaaaaa agcccttcat
     9721 gcggaaccca ttcgatttat gacggaagtt tttccggaat aaaaaaaaat ggtgccggag
     9781 tggctactgc ttattttatt cagcaaaaaa tgactgtgct ctctgatgaa gaatggctga
     9841 aaactagagg tgagcagaat ggaaatcgaa aagaccaatc gaatgaacgc gctctttgaa
     9901 ttttattcga ccttattgac agaaaaacaa atgaactaca tggaattata ttatgcagat
     9961 gatttttcat taggtgagat cgcggaagaa tacgaagtaa gccgacaagc agtatatgac
    10021 aatatcaagc ggacaagcaa gatcttggag gattatgaaa aaaaactcca tcttttttct
    10081 aattatattg ttcgagaaca ggtattggaa aatatgactg catatattac agataagtac
    10141 ccggaagata aaaaactact tgaatatgta cagcaaattc aagcaatcga agaataaata
    10201 aaaggaatga agaaaatatg gcatttgaaa gcttaactga acgtctccag caggcgatgg
    10261 gaaaaataag aaaaaaagga aaagtatcag aagctgacgt aaaagaaatg atgcgggaaa
    10321 tccgccttgc cttactagaa gccgatgtta acttgcaagt agtcaaagat tttactaagc
    10381 gcgtacgcga acgtgcggta ggggcagaag tgctggaaag tttatcacct gcacaacaaa
    10441 tcgtaaaaat cgttgatgaa gaattgacga tcacacttgg ttctgaaaca gcagagttaa
    10501 ataaatcacc aaaaatacca actgttatca tgatggctgg tcttcaaggg gcaggtaaaa
    10561 cgacctttac tggtaaatta gccaattatt taaagaaaaa tgaaaatgct cgtccgctcc
    10621 taatcgcagg ggacgtatat cgaccagctg cgatcgacca attgaaagta ttaggtcaac
    10681 agctagacgt tcctgtattt gatatgggaa ctgaagtcag cccggtagag atcgtccgtc
    10741 aagggatgga gttagcaaaa gaaaagaaaa acgactatgt attgatcgat acggcaggac
    10801 gtctgcatat cgatgaaaca ttgatggatg agttgaaaca aatcaaagaa ttgacgcaac
    10861 caaatgagat tttgcttgtt gtcgatgcca tgactggtca agatgccgtc aacgtagctg
    10921 atagcttcaa ccagcaatta ggtattacag gggtcgtcat cactaaactt gatggggata
    10981 ctcgaggcgg ggcagcacta tcgatccgct cagtgacagg cgcaccaatc aaattcatcg
    11041 gttctggtga aaaattgact gacttagaag tattccaccc agatcgtatg gctagccgta
    11101 tccttggtat gggggatatg ctgacattga ttgaaaaagc gcagcaagac tacgacgaga
    11161 aaaaagcgga agaacttgca cagaaaatgc gggaaaattc attcgatttc aatgacttta
    11221 ttgaacagct tgatcaagtg atgggtatgg gcccactgga agatctaatc aaaatgatcc
    11281 caggaatgaa tcaagtccct ggaatcgaaa acgtcaaggt agatccaaaa gatgttgaac
    11341 gcaaaaaagc gatggtttat tctatgacgc cagcagaaag agcaaatcct gatctgttga
    11401 acccaagccg tcgtcgccga attgctgccg gttctggaaa cagtgttgtt gaagtgaatc
    11461 gaatgatcaa acaattcaag gaatcaagaa aaatgatgca gcaaatgtcc aaaggggata
    11521 tgaacatccc aggtatggac caaatgtttg gttcaggcgt caaaggaaaa ctaggcaaaa
    11581 tggcgatgaa tcgaatgatc aagaaaaata agaagaaaaa gaaaaaacgg aaataagatg
    11641 aaaaaaacag cttcggtaca gactgaaaat gtctactgcc ggagcttttc tatcagcaat
    11701 ttttattctt tactaataca attactctaa acgaagaggt ggcacagatg agatgtccgt
    11761 ggagtgaaca aacagaaagt atgcggcaat atcatgatac tgtctggggt gttcctgaat
    11821 atgatgacca aaagctgttc cgaaaattga tgcttgatat taatcaagcc ggattgagct
    11881 ggcagacgat tttaaataaa agtgaagcat tcgatgaagc atatgatcga ttcgatatca
    11941 aaaaagtcgc aaattatgga gaaacaaaaa tcagtgaact aatggaaaac aaaggaatta
    12001 ttcgtaaccg aagaaaaatc gaagcagccg tccaaaacgc caaagcagtc ttgaaaattc
    12061 aagaagaaat cggcagtttt tcagagtatc tttggtcgtt ttctaatgga aaaatcgttg
    12121 attcttctta tcattcacaa gaagaagtcc caacaacaag tcctctttca gatgaattag
    12181 caaaagatct aaagaaaaga ggatttaaat ttatcggaag cacaacaatc tatgcttttt
    12241 tagaagctgt cgggatcatc aatgatcacc ttgacagttg tttccgcaaa gaagaggtcg
    12301 tacgtatcgg cagaaagggc gaataaagca tgcgaaaaat catattttat ggtgctataa
    12361 gtttagatgg ctatttggca acgaaaacgg atgatcttca atggcttttt gacactccaa
    12421 caggtaagca aactacttat gaagcttttt atgaaacaat cgatacgacc atcatgggca
    12481 gaaaaactta tcaggaagca aaaaaatatt tggagaccga agcaatttat ccagagaaaa
    12541 agaatttcgt tttttccacc cacgcacaat tacaattgcc agatgctgag gtcgtaaacg
    12601 aagatcctgt tgcttttcta aaaaatctac aaatgacaga aggaaaagat atctgggttg
    12661 tgggtggcgg tcgattgatc aaaccgctat tggaacatca aatgattgat gaatggtata
    12721 tccagattac ccctgtatta ttaggggacg ggattccttt attccaaaaa ggaacatatg
    12781 aagcgcgttt tgaaatgcta gacacgacac gattcggtga atttatcgaa ttgcattatg
    12841 tacgaaagta gaagtaaata aaataaaaaa ggcaactata tgcagaaatg tcctgtacat
    12901 agttgccttt tttattaccg ttatgctttt tactctgtta atttgttaga aaaatctaga
    12961 tacgcatcta aaatcgtttg gaagaaactt aatgttcctt caacgatatt accattttca
    13021 tcaattagat tcgcaacgtt tccgatatat gcttccggtt gctgcaaggt tggcacattc
    13081 aaaaagacaa gtgattggcg tagatggtgg tttgcgccaa agccgccgat cccacctggt
    13141 gatgcagtaa cgactaaacc tggtttatga tcccaaacac tgtgtccata aggtcttgag
    13201 cctacatcta aagcattttt caatactgct ggcacagaac ggttgtattc aggagtaacg
    13261 aaaacgacac cgtctagtcc cctcacttcc tcacgaaaac gagtccattc agcaggagcc
    13321 tgttctggag tttcgagatc ctcattgtaa aatggcagat catcgatttt tacaaataca
    13381 ctttcgtacc cttcaggaag caatttcgca aaagcttctg ctactttttt gttgtaagaa
    13441 tcttttctta aactaccaat aaaaaatcca attttttttg tcatacgatt tttcctcctt
    13501 caaactatct ataataatct tacaaaagat aagtaactgg agcaaataaa aacacttact
    13561 gacaaaagct acatagaaga tagattactt atttttaaca aaaaagaagt ctaaaatctt
    13621 ctattttcta aaaaaaatcg aatttaatca agctgtaaag aaaaaaagct ttacagggta
    13681 aaaaaaaact gttacactac atcttgtgaa taaaacaatg gaggtggaaa taaataatgg
    13741 cagttaaaat ccgtttaaaa cgtatgggtt ctaaaaagag tccgttttac cgtatcgtcg
    13801 tagctgattc acgttctcct cgtgatggac gtttcatcga aactgtaggt acttacaatc
    13861 ctttgaaaga ccctgcagaa gtagttttaa aagaagactt agttcttgac tggttatcaa
    13921 aaggtgcaca accttctgac actgtacgta acatcctttc aaaagaaggc gttatgcaaa
    13981 aacaccacga agctaaattc tcaaagaaat aaggtgaccg gatatgaaag atttaagcga
    14041 cttagtctta acaatcgttc gtccgctagt cacttatcct gatcaagtca agttagacgt
    14101 cgtagagtcg aaagactttt acgagtacaa tctaacagtg gctcctgaag atattggccg
    14161 tatcattgga aaacaagggc gtgttgctaa agcaatccgc acaatcgttt acggtgttcg
    14221 gatcaatgca ccgaaaaaag tgcgtttgaa tatcatcgat gataaggaaa agtaaataaa
    14281 cgtgctgtac tcgaattttc gagtgcagct tttttgtttt ttttattaat tgaagaaatg
    14341 attatttggc aaaacaagca agaaagcgta tccttatagg aggagggagt gaacagccaa
    14401 tgagaaagaa aatgttattt attccatttg ctctattgtt aagcgcttgt agttcggtta
    14461 ctcaagaatc agacaataca catctatcaa gcagtatatc tgaaactgtt accagtccag
    14521 aaaaaagtag taccaaaacg gcttccacca ctgaacaaac aatggctacc tcaaataatg
    14581 agaaaaagac tgctttagac cagttgaaag aacaacagcc aaatgttccc atgcctctag
    14641 atgtaccagt ctcttcaggc tatctcaata tcgcagcgac acatacaaag caaggttatt
    14701 ctattttata ttacagaaca gatcgtcccc tcgggttgaa tgctgacgaa ttgaatcaag
    14761 aaacgcctat tgcaacctat ctatatcaat atgggtttgc ttctagtcaa gaaacaatcc
    14821 aagttttgca gccatttgag atcgacacaa atggtcagca agtggactta ggttctcgaa
    14881 tcacagggta tcagcaaggt gcagcaggtt caagttttct tgaatggcaa gaaggaaact
    14941 ggcgtattcg aatccgtggg aataatatag aaggccaaga tcccttacta ttagctaaag
    15001 aaatcgtagc ttatttggaa gaaaacagtc taccagcacc tgaacaattt ggtaaaatta
    15061 ctgttgatat gggtgatacg acgaatcgag ccgtagaagt aagctggcaa gaaccgaaaa
    15121 atgcctatac gatcactcat caagatccta tgtctgcgtt gaagatggca gtttctatga
    15181 agaggttgtg aaatcagcgc gctgatctga gcccatcatt taactccaag tcacaggaag
    15241 taatcaaaaa tagcacgccg catttttgca taattttggc ttaatacctt aaaagaaaac
    15301 tagaattgcc tcttcacaca tgctaaaata aaacagacaa aaaagaaatg gggatataag
    15361 ttgactgaat acttgaatgt aggaaaaatc gtaaacacac aaggtatcaa aggcgaagtc
    15421 cgtgttatct caacaacgga tttcccagaa gaacgttaca aaaaaggaac ggtgctaaca
    15481 ctattccaag acggaaaagc acctgtggaa ttaacagtca aaagtcatcg caaacataaa
    15541 aattttgact tattgagttt tgaaggacat ccttctatca atgatgtgga aaaatatcgg
    15601 gatggcatat taaaagtgtc aaaagacaac tctgtagatc tagctgaaaa tgaattctat
    15661 taccatgaga tcatcggtct gcatgtatta gaagatggaa ctgagttagg taaagtaaaa
    15721 gaaatcttgt caccaggtgc caatgatgtt tgggtcgttc aacgtccgaa aaaagctgat
    15781 atattgcttc catatatcgc ttctgtcgtc aaagaagtcg atttagaagc tggagtggtc
    15841 cgtgtagaaa ttccagaagg actgattgac gatgaagatt gatgtattga ctttatttcc
    15901 aagaatgttt gaaggtccta tgggggaatc catcatcggt aaggctgtag ataaaggatt
    15961 attagatatc aatatctcta acttccgcga ttattcagat aacaaacatc agacagtaga
    16021 tgattatcca tacggcggtg gagcaggtat gctgctcaaa gtccagccca tctatgataa
    16081 catccaagcc attgaaaaag ctgcaccgga tgtcaaaaaa agagtaattc ttcttgaccc
    16141 agctggtaaa cgattcgatc agaaaatggc agaagaattt tcacaagaag aacacttagt
    16201 attcatttgt ggtcattatg agggatatga tgaacgtatc cgcacattag tgacagatga
    16261 agtttcctta ggagattatg tccttactgg gggagaatta ggtgcgatgg tcatgatcga
    16321 tgcaacagtt cggcttttgc cagatgtgtt agggaatcag acttcagctc aaacggattc
    16381 ttattcaacc ggtttattgg aacatcccca atatactcgc ccggcagaat ataaagggat
    16441 gaaagtacca gaagtattga caaatggaaa tcataaattg attgaagaat ggcaattaaa
    16501 agaatcacta agaaggacct atcaacgtcg gcctgatctt ttggaaacac ttgaatggac
    16561 acctcaaatg acgaaatttt tagaagaaat caagaaagaa gaacaagaaa aaacagcaga
    16621 tgaagccaag tgactttttc taaaatcttc tttacagtag gttgataata tgttagtatt
    16681 ttatggtgag tgcaaaagca cttaactatt acgatattcc gctgtgagaa aagctcataa
    16741 gaatatttgg aataaggaga agaaaaccat gaatccatta atcgaagaat taacaaaaga
    16801 acaattacgt tctgacatcc cagcgttccg ccctggtgac actgtgcgtg ttcacgcaaa
    16861 agttgttgaa ggtactcgtg aacgtatcca gttatttgaa ggtgttgtta tcaaacgccg
    16921 cggtgctgga atcagcgaaa cttatacagt acgtaaagtt tctaacggag tgggcgttga
    16981 acgtacattc ccattgcata caccacgtgt tgcaaaaatc gaagtagttc gctacggtaa
    17041 agtacgtcgt gcaaaattgt actacctacg tgcattacac ggaaaagctg ctcgtatcaa
    17101 agaaatccgt cgttaatcat cgttatcaga cagagaaaga acccttggta ttcaagggtt
    17161 ctttttttat tatctgtttt tttatattaa cgatttctat ttggtacatt tttgattaat
    17221 aaaaaggggc aaaaaagggg caacaagtta cagtttttct atatcttctt tcaatttttc
    17281 ttttactcct tttgtcacat gcaaataaat tttttcagtg atatcactat tttcgtgacc
    17341 aacgcgatcc tgaatggcat acagaggagt tcctagttcc gctaattttg aaatatgggt
    17401 atgtctaaaa atatgtgaac taagtttttt atcaatcttc atgtcagctt tatgacttct
    17461 caaataggtg ttaattgctg ttagttggaa aggagttcct ttcgttgttt gaaatagaaa
    17521 ttgaccattt ggatttaatt ctagaagctc attgtaaata gctatcgctt tttttggtaa
    17581 atcaatttct cgcattccgg cagctgtttt tgtagaatca gattttttca tatcagctat
    17641 tgaacgttct ctatacatca tcgtcccatt tataactact gatgcattat tattggtaat
    17701 gtgtacatca tctttactta atgcgattgc ttcaccagac ctcatcccag tcaaatatag
    17761 ccactggaaa aggagagaat atcttttatt gtgtgaagtt gtgaaatcta ctagtctatt
    17821 atattcatca tcttctaaaa atttatcttt aatttttatc gtttttgact ctcttttata
    17881 atcaataact acttcatcga taggattttt ttctacataa ccttttttca tagcatatga
    17941 aaaaagaaga ttcaatttgg atttaattac actgacgtac ttatttgaca agtcatcttt
    18001 atatatcatg tcttcaaa
//
LOCUS       DACDIG010000055        16334 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.055, whole genome shotgun sequence.
ACCESSION   DACDIG010000055 DACDIG010000000
VERSION     DACDIG010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.055"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(92..490)
                     /locus_tag="IV247_001997"
     CDS             complement(92..490)
                     /locus_tag="IV247_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAP8778059.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(487..1260)
                     /locus_tag="IV247_001998"
     CDS             complement(487..1260)
                     /locus_tag="IV247_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP8778060.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1257..1940)
                     /locus_tag="IV247_001999"
     CDS             complement(1257..1940)
                     /locus_tag="IV247_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP8778061.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2107..2994)
                     /locus_tag="IV247_002000"
     CDS             complement(2107..2994)
                     /locus_tag="IV247_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8778062.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(2991..3860)
                     /locus_tag="IV247_002001"
     CDS             complement(2991..3860)
                     /locus_tag="IV247_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8778063.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(3997..5283)
                     /locus_tag="IV247_002002"
     CDS             complement(3997..5283)
                     /locus_tag="IV247_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8778064.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5556..6134
                     /locus_tag="IV247_002003"
     CDS             5556..6134
                     /locus_tag="IV247_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP8778065.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6134..7063
                     /locus_tag="IV247_002004"
     CDS             6134..7063
                     /locus_tag="IV247_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8778066.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7060..8796
                     /locus_tag="IV247_002005"
     CDS             7060..8796
                     /locus_tag="IV247_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8778067.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8859..9695)
                     /gene="pheA"
                     /locus_tag="IV247_002006"
     CDS             complement(8859..9695)
                     /gene="pheA"
                     /locus_tag="IV247_002006"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAP8778068.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9692..10201)
                     /locus_tag="IV247_002007"
     CDS             complement(9692..10201)
                     /locus_tag="IV247_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAP8778069.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10194..11489)
                     /gene="aroA"
                     /locus_tag="IV247_002008"
     CDS             complement(10194..11489)
                     /gene="aroA"
                     /locus_tag="IV247_002008"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAP8778070.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11502..12593)
                     /locus_tag="IV247_002009"
     CDS             complement(11502..12593)
                     /locus_tag="IV247_002009"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAP8778071.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12610..13776)
                     /gene="aroC"
                     /locus_tag="IV247_002010"
     CDS             complement(12610..13776)
                     /gene="aroC"
                     /locus_tag="IV247_002010"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAP8778072.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13778..14842)
                     /gene="aroB"
                     /locus_tag="IV247_002011"
     CDS             complement(13778..14842)
                     /gene="aroB"
                     /locus_tag="IV247_002011"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAP8778073.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14875..15897)
                     /gene="aroF"
                     /locus_tag="IV247_002012"
     CDS             complement(14875..15897)
                     /gene="aroF"
                     /locus_tag="IV247_002012"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAP8778074.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         4709 a         3619 c         2904 g         5102 t
ORIGIN      
        1 ataagtattc acgagaaaaa tacaaacaaa aaagcggaaa taacgctcat ccgccttctt
       61 gtttgtgttt atttcctctt caaacgcttg tctatgattc agacttttct ctatcgttat
      121 tttcatctaa aagttcttgc attccgataa tttctcgata ttgatgaatc ttagtgatac
      181 caatgacttt gtctacatca atactaaaac aaataccatt acctggaata tcgatgttga
      241 tgccttgacc aatcgcatcc attactttat tcgtcaaact atcgggtact aatgtcaaca
      301 caatatcttt ttctggcgaa atgtccaaac ctaaaaattt ccctgaatca tgtacaccag
      361 aacctcttcc atgtaagatc gttccgcctg ctgcacctgc ttcttttgaa aagtcgataa
      421 catcttcgcc cattcctccg tcaacgatcg tcacaatcat gtgaagattc aatgctacaa
      481 gatctttcat ttgctttccc ccaatttttg caatctttct ttttcgattc tgtctttttc
      541 atcttttcgt ttataaatat atcctagtac taaagtggat aatattggtg tcaatgcaat
      601 cattgcaacc acaccaaacc cgtcaatgat cggatctctt ccttcaattt gtgcagcgat
      661 tgccacgctc attgataaaa taaatgtcga acacatcggg cccgttgcta ctccaccgtt
      721 atcaaaagcc attgctaaaa agatcttgtc cacttttcgt cctaaaataa atgctagccc
      781 gtatcctggg atcaagaaat aataaagaga gaatccgacg agcaaacgcc acatagacaa
      841 tcctactgct gcgccgatcc caattgaaat taccatcagc atgattttcg atcggattct
      901 cccctcgctc aattcttcaa cctgtttgat cattacatga atcgccggct cagcaaaccc
      961 tactaagaac cccattaaaa agccaagggg aaccagtata taattgtaag gcaatcccgc
     1021 aatagcactg cctaaatgct gtcctaccgg tacataagcc atattgactc cgtgcaggaa
     1081 aaggatcaac ccaatagtcg ttatcaagaa acctttcata atgtcaaaga aacgcttttt
     1141 gcgcatacga aaactaatga tattcattaa aacaaatagg attacgatag gtgccactgc
     1201 caaaatgact tccatggtaa cgatttcgat tccatctatc acttgctgcc agaaactcat
     1261 tggaagatca cccccaaaat catcacggca agaatcggcc ctaacgaagc aataccgacc
     1321 attccaaaac tgtcattttc atttttcttt tgtttgatca tactagtcat ccctccggca
     1381 agggccaaga taaatggaac agttactgga ccagtagtca ctccccccga atcaaaagca
     1441 atcggcataa attcggaact ggtgaaaaat gaggcgatca aaacgcctat gtagcctata
     1501 gccatcaatt gataatagga tagcttgaat acgacccgaa gcagcgcaaa ggctagaaat
     1561 actccagtgc cgacgctgac gatcccaatc agcaacactc tgccaatctc tccgtcagat
     1621 atatcatata cttgttgtcc caaaacttgc acggaaggtt cagcaatcgt gatcacgatc
     1681 ccgatagcaa atcccaaact gaccaaaacg gtcaaacttt tctttttgat catatatttc
     1741 cctactagat ctccgacttc catcatagaa taatctgctc caaacaaaaa gagcgtcatt
     1801 ccgatcatca tgattgccgc accaaccaga aatgaagcga ggtcttctcc ttctaacggt
     1861 gcaaaaacaa acgttaaaat gacaatcagg acagtcatcg gcaatatagc agcaatcact
     1921 tctttaaaat tttttagcat gtatcctcct cctttactct ttaagtttac cttaaaatga
     1981 aagagttttc gagcacaaac ttaaaacgtt atcatttaat ttttctcaac ataaaataac
     2041 acacgtccca aaagtatctt tttacttgga aaaatgactt tgaaggaacg cgcttattgt
     2101 gtttttttat tttacagagc cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa
     2161 tacgatggca gttggtaaag tagcgatgac taatccagcc atgatcattc catagtctgg
     2221 tgtataggaa gcacccatag cagataaaac aagcggaacc gtccgtttat caggagattg
     2281 caaagctact aagggccata aataattgtt ccagctactc atgaatgtga tgattgcagc
     2341 cgcggcatag gtatttttag ccgtcggcat atacactcta aaaaaaatac ttagttcttt
     2401 tagaccgtct aatcgagcgg cttctactag atcttttgga aaagatttaa cattttgtcg
     2461 aaagaagaag atcaaaaaag cagtgctaac agatggcagg atgacaacca agaaactgtt
     2521 gattcccaaa accgttccgt tcaattgtga aaacattcga tataaaggga tcatcaatgc
     2581 cgcaaaagga accatcatcg atagcagtag aatattgaaa attaggtctt ttcgtttact
     2641 ttgataaatc tcaaatccat atcctgccat tgacgaaatc agcaatgcaa acacagtggt
     2701 caccaaagcg atcatcgctg aattccaaag ggaacgagta aaattcagct cattggaaaa
     2761 caaattttga aaattgatga tcaagttact gccaatcttt atttttcccg atgtgatatc
     2821 cacgggtgta ttcgtcattc ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga
     2881 taggatgctt aatgctgtat actttaatac tgacccaatt gtcgaacgaa gttctaatgc
     2941 ttgttttttg acgttggggc gttcgattgt ttctttttct accatatttt tcatcgactt
     3001 tctcctccta ctttcgtttg gattatcgac aatatcacaa taaaggaaac aataacaaag
     3061 gaaactgctg ccgcatatcc aaaattaggc gtgaatttat aactcaaatt atagatgtat
     3121 tgagataggg tggtcgttgc atttgctggt cccccgccag taatattttg tacttcatca
     3181 aaaagctgca atgtcccgat cgtcgaagta atggaagtaa acaaaatgat tggttttaaa
     3241 tttgggatcg tcactttgat aaactgctgc catttagaag caccgtcaat agatgctgct
     3301 tcataaatct ccgaatcaat attttgcatg cctgaaagga aaaaaatcat attgtatcca
     3361 gtccaacgcc aagtgatcga aagaatgatc agaactctcg cccaaattgg atgagtcaac
     3421 cagccaatcg gtgtgtcaat cataccaatg cccaacaaca agttgtttac aaggccagat
     3481 tgagaaaata gacttttcat gatcagagaa taactgacca tcgaagtgat acatggaaga
     3541 aagatagcgg ttcgaaacaa ccctttatat ttcaactttt tatcatttaa aaggttggaa
     3601 ataaaaagtg ctagaaacaa catgatcggt acttgtacaa gtaaatacag gaatgtatta
     3661 aaaaaagctt ttttaaaagt tgcatctgtc aacatccgct gataattacc taacccgtta
     3721 aatgtcatat tgacaggggg accactttga aaagacgtga tcaaagccaa aaccatcgga
     3781 ataaaaacca tgattgatat caaaatgact ggcaagatta aaaaagcatg cccgtttttg
     3841 aataaatctt tctgcttcat tcttttccct ccaattctat cattgattat ctacatgaaa
     3901 agacaagaaa gacaatatct gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg
     3961 atgaaaaagc gaagaaattc acttttttac caccgatcaa ttagccaatt gtgcttctac
     4021 ttgtttttgt gtatctgcta gtaccttgtc tgtctcttct ccattgatga ttcgatgaag
     4081 agcttcagct acgactgctt caattgcata ggtctcatgt ccgtaattca cttctggtat
     4141 ttcagatgtc catgttgaaa aatcgtcaaa gatcttttga ccactgtaaa attcagaagc
     4201 ttcttgataa gcttcttgtt cattcgcgct aagcatcgta gatactagtc cgatttcttt
     4261 cgcaagagtg cccatcagtt cttcattcga agcaaacgtt gcttctagga aatctttcgc
     4321 tgaatcttct ccagctactc ctttgatcac ataccagcca gcgccgccaa ggttagaagc
     4381 ttgcgctttt tgcagatttt ctggaagtgc aggtattggt gcaatcttcc attttccaga
     4441 ttggttttct gctccttgga tcgttgaaga ataccaagcg ccagttgggc tagaagctac
     4501 tgctcctgat tgtatcgcag ttactccagc attccagtca gaggtttgtt cgactagatc
     4561 ttctttcaaa agagtggcaa acaattcaag accatacttt aaagatgcat tgtcttggat
     4621 cgtgactgtc tccccatctt cagatgtata ccattctcct gcctgttgca taatcatcct
     4681 tactcgacca aggtcagaag gattgacttc agcgattttc tttcccgtct tttctttgac
     4741 atctcgcgct acttgaatat actcgtccca cttcagattc tccatatcct cttctgtata
     4801 acctgcttct gccatcagat ccgtacgata gaagtttgct gttacgcccg aatcgaaagg
     4861 aacaccgtat attttatctc ctactttgtt taccgcaaat ttatacggtg caaaatgatc
     4921 ttcctttaca atagcagaca aattagcgaa tgcatctgga taagaagtca aatagccttg
     4981 tatcctataa tcttcaatca agacgatatt gggcaatcct tctgtattcc cgctcgctaa
     5041 agcggtgttc agtttctgaa cgatatcatc ctgagacatc gtaacaactt ctatctctgt
     5101 ttcttcgttc tcataaactt tttttgcttc attcactgct ttgatattga atgtctcatc
     5161 ccacgcccag acagtgactt tttcttcttt gtctgctgta ctactagcac ttccgcatcc
     5221 tgttaagaaa cctgatgctg ccgctaaaca aacgactcca gcaattaact ttctgatgtt
     5281 catttttatc cgctcctttt ccatccatta gttgtttacg tttgctacat tcatcatacc
     5341 aaaatttgaa aacggtttct ttattattca gatacttcaa aaatttctcc taaaataata
     5401 aaaaaataca aatcaactat gacaaaagga ataatcttag catctagacc aaagatcgaa
     5461 atggtgaggt ttctttaaaa taaaaatatc cttgcatttt ctcctagaaa taagtatttt
     5521 taaattacaa gactattctt aaggcgggta aaaaaatgcc aatacgcgta attcttatcg
     5581 atgatgaaca gacgatttta gatggaatga aacagttgat caattggtct gagcacgggt
     5641 ttcaattaat agggactttt tccagtccgc tgaaagcgct tgatttttct ttggtacatg
     5701 caatcgatat cgtggtcaca gatgtaatga tgcctgaatt aaatggaatc gaattatcgc
     5761 ggctattaaa acagcaacaa cctgaaacac agatccttat cctctctagc tatgatgaat
     5821 ttgatatggt taaagattct tttaaagaag gcgtcgctga ttatctttta aagcctcgat
     5881 tgagtcctga tttttttcta aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc
     5941 ccaacaaaag cccaatcacc aatcgttatg agcagatctg cgagaatctt tcacagcaga
     6001 ttgctggaca aactcgtcca ttgcttgacc ctcttctttt tgaacacacc ttgtttttct
     6061 gttatatacc gaaaaacggc cattcagttc aaaacaaggc tctaaacaaa aacagtcgat
     6121 cacccattca ttgatggata gcgacgattc gtttactatt tatcctgttt ctaccttttc
     6181 ggaagaatcc ggctatgtaa tcaatactga ttccaaagat acacttagga agtttgttca
     6241 aaagtttcgt caagaagttt ttccaggtga tttttttgtt gtttctgatt cttttgcaat
     6301 ccaagatatc aatgaaatat tccaagagat cagaaattgc accaaaggac aacgtttcta
     6361 tcataaggga aagaaccttg cttatcagca tgaacttctt ccgttattaa ccaaccacga
     6421 gcagcaagca agtagttatt taacaaaaat cgttcataaa gactacgctt tagctgtcaa
     6481 agaacttgag aatttcttca cgacgatatt ggaacagcct attgatccag cttatttgaa
     6541 acatgaggca atcaatcgtt tctatgcatt actcaacgct ttggctgacg agcatgttgg
     6601 gaatgaagaa ctttctttgc tcaagatcgc ccttccttct ttattagccg atgctgagta
     6661 tttcgaagat ttttctattc ttgtcaaaga aacacttgag aaactcaccc acttttctgc
     6721 aaatgcaaaa aaagacaatt tcgatatctt gactttgatc tacgagttcg tccaggaaaa
     6781 ttatgagaaa gaaatcaatt tacaactttt atccgaaaaa tatcattttt catatagcta
     6841 tctctcagcc atctttactg aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt
     6901 ccgtatcagt aaagccaagc tctttttgac ggaaacacgg cttactttga cggaaatctg
     6961 tgagaaagtc ggctataccg aactgggtta tttctccaga gtttttaaag aagagacagg
     7021 aatgacacct tctcaatacc gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag
     7081 catgaactct ttgccaaact tctgttcatt ttgttcatcg gtctttttct gcaagccatc
     7141 gtcatttctt tgtttattta tcatcgatca agggatgcct atatccagct gttcaatcaa
     7201 tcgaatgatg tggtactaaa aaagattcaa tctgaatttg aaagtttgaa tgatacaatc
     7261 gagaatacat tagccgcttt tgattctaat ccagctgtca aaagctattt ttccaatgat
     7321 ccagcccaac atatggaaca gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct
     7381 ctttcaaaaa tccatccaat gatcgattac gatgtattga tctttggaga aaatggaaga
     7441 acttttgtcg gaaacgacat gctcactgcc gtatctgccg attctttttt tcaatctgcg
     7501 attgctcaga gagtgaatga acgtgctgct gatacacaaa tgctttttgc acaccacgga
     7561 ttgactcttc gcgataagaa agcacccagc gtcttctttg tcaaaaaact gaaaaataca
     7621 ttgaatcacg tttacggata tgctgtttta tcgatctcat ccaagcaact agcgaatctt
     7681 tttcaatctg tagtaaatcc tgagatatcg aagatctcct tcatgaacga agaacagacg
     7741 atcatcgcat caaatgaaga aaatacaatt gggagccgat caatgatttt caaaaaacta
     7801 acagtaggtg aaacaacgat ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc
     7861 caggattgta cattactcag tcaagtcgat cttcattctt tatctaacca aatgggcgtg
     7921 atttttccga ttattttgta taatatcgtg acacttgctt ttgctggctc attggtattt
     7981 atgtatttgg atcgacacac aaaatcgatc taccgcttga ttgattcgct aaaacggcta
     8041 gaggaagatg gcttaaaagc tactgttcct gtacaaggaa gccatgaagt ccaattgcta
     8101 gggaatacga tcaatcaatt gctaagtaat gctcagcatc attatgaaca gacgatccaa
     8161 gacgaaaaga agaaacgttc gcttgaaatc caaactatgc aagcacagat acagccccat
     8221 tttatttata atacgctgac tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa
     8281 gcagttcaag gaatcgacag tttcattact ttactaagaa gtacgattgg gaaaaaagaa
     8341 gaagtgattc cagtcaagga agaaatcaaa agcgtacaaa gttacatcga tcttttgagt
     8401 atgcgatatg gcgaaagaat ctcggctaaa ataatgattc ctgatgaact gcagtcatta
     8461 tctatgccga atatgatcct tcaaccaatt atcgaaaacg cctatcttca tgcttttcac
     8521 aataaaacgc aaggattcat cacggtgtac ggcaaaacaa aaaatcagct tcttttattc
     8581 gaagtgattg ataacggcga tggcttcgat actgaaaata ccaaacaaaa aaatgatcat
     8641 ttttcaggaa tcggcatcag ccatgtagat gagcggatac gcctaatgta tggcgatgca
     8701 tatggcttaa cgatccaatc agtgattggg acaggaacga ctgtaacgat ttgtctaccg
     8761 cttcttccta aaaatcaatc agaatatcaa aaataaagaa tagccaagcg ctgatttttt
     8821 tattttaagt taaataaatc aatcagtatt cttgactttc atctacgcgt ctcttctact
     8881 gtcagcactg gataacagcc caatatttga ctttctcctc cgagcatctt gatttcttgg
     8941 agcgcattct ctactaatgt cattggacgg tccaacaaca aatcgatcac gaaaaaatat
     9001 tctcccaagc tagttttcaa tggtcgggat tcaatcttac ttaagttgat ttcccgccag
     9061 ccaaaagctg ccagcatttt atgaagcatc cctggccgat tagcaggtag tgtcaagatg
     9121 acactcattt ttactggagc tgactgctgg ctggtcatct ttcggtcacc gacgatccaa
     9181 aaacgtgtct ggttcaactc attatcttgg atattctcag ctaagatctc caatccaaca
     9241 tgctgtgctg tttctaatga agcaatcgct gctgcgtctt ctttcggatg ttctgctaca
     9301 tacattgctg cagcagttgt tgattctgtt gctactaaag gaacattagg ataatgcgtt
     9361 tctaaaaact gttgtgattg tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt
     9421 gttgctggat tgcctaaaag ctgctgcttg ataggaagaa cgatttcact tttaacttcg
     9481 acttctggat gtttggaaag aagatcgatt gtgtgatgaa cagctccttc caatgaattc
     9541 tctacaggaa cgacagccag atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg
     9601 atggaagcat acgcagtcaa ttgttctgta ggaaatagct gacaagccgc ctgataagtg
     9661 aatgaacttt ctggccctag atagctgacg ttcatatgct tttgatcctt tctataatgg
     9721 tatcgatgat cttttctggt gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt
     9781 cataccaaga aagtcgttgt gccaataatg cttttatttc ttctcttgtt tttccaagag
     9841 ctaaaggtcg aatagacgtt tcatcttgtt ggatacgatc aactagacag tccgcttctg
     9901 cctctaagta aagaacaatc ggctgtgttt ttaaaaaacg ccgattttct gcactttgga
     9961 caatccctcc accagtcgcg ataattttgt cttcatttga cagctttctt aaaatatctg
    10021 actccacttt tcgaaagctt ttttcaccgt aatagtgaaa atattcggca atcgacaatt
    10081 ggatatgttc ctcaatcttt gtatctaaat ctacatactc tttttgtaaa cattgagcta
    10141 accctttacc gattgttgtt tttccagcgc ccatgaaacc aatcagcaaa atgctagcca
    10201 tgagcttctc ctcctgacac aagttctgct aaatcgtcaa aaaatgccgg ataagagatt
    10261 gaaacagctt cagctccttc tagttcgcaa ggtccatcag ctatcaaagc agctacttgc
    10321 agcatcatac cgattcgatg atcgcctctg ctggacactt ttgcgccatg cagaggtgtc
    10381 ggcccatgga tgatcagacc gtctgctgtc gcttttatct ttgctcccat tttctgtaat
    10441 tcttctgcca ccgcatcaat tctgttggtt tcttttactt ttaattcttc tgcgtctttg
    10501 atgatcgtga ttccttctgc ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc
    10561 agacgcggaa tgatttctcc accaatcgtt gctttcttca aatgactcgt cttgacactt
    10621 aaatcagccg attggttctg ttcattgtga ttcgtctggg taatcgctgc gcccatctcc
    10681 tccaacacat ctaagatacc agttcttgtt ggattgaccc cgacattttt taacaacagt
    10741 tggctttccg gaacaagaag tcctgctgct agaaagaaag cagctgatga aatatcacct
    10801 ggaacagtta tctgttgacc agttaatttt tgcggtccgg tcactatgat cgttttatct
    10861 tccactgtga tccttccgcc aaattgacga atcatttctt ccgtatggtt acgggaacgt
    10921 tctttttcaa cgattacgga tgtgccttct gcttgtaatg ccgcaaacag caaagcagat
    10981 ttgacttgcg cactggctac aggcatattg tatcggatag gtgataaaga gcgggttcct
    11041 gtcacagtga ttggtagata ctggtcattt tcttctcctt gcaagtcagc gcccatctcg
    11101 cgtaaaggtt ccatcactcg ccccattgga cgtttagaca acgaagcatc cccgaacaga
    11161 gtagtcgtaa aaggctgtcc agccaaaata cccatcaata aacgtatggt cgtaccggag
    11221 tttcccatgt caattgattc ggttggttgg acaaatgagt ggctaccgcg accatgaatc
    11281 ttgatcacct cttcctcttc ttcgatctcg acacctaatt gttggaaggc atgtaaagta
    11341 ctcagacaat cttctgcgcg caaaaaattt tgtatagtcg tcgttccttc agcaattgct
    11401 ccaaacatga tgctgcgatg ggaaattgat ttatctgctg gtatcctaac agtccctctt
    11461 aatccatgta tttgttgtaa taattgcatt tatccagcct cctatttgat ccagcaatga
    11521 taactggtat tagacataat gattcttttt gcttgtaaca aatcctgttg gtttttaaaa
    11581 ctgatttgca gtattcctaa aatatcttcc ctggtctctt ggatcttcaa attaatcaaa
    11641 gaaactcccg cttttccgat cagtccagtt acttctgcaa taacgccagg ttcatctggc
    11701 acatcgataa ataaatcaaa aaaggcagga atcgcgcctt gcttatggac aggcaactgg
    11761 tctcgacttt cttttgcccg attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg
    11821 atccattctg ctatttgtgt catttcttgt tgccaatctg ccaatcgttc caataatatc
    11881 tgcttattgc tcaacaaaat atctgtccac atgacaggat ctgaggaagc gatcctagtg
    11941 atatctcgaa agcctcctgc tgccagttgt ttggctcgcg gatactcttg attgaattgt
    12001 tctgcctgat tgactaagcc agctgccaga atatgtggaa aatgacttag cattcctgtt
    12061 atacggtcat gttctcttgc tgtaagagtt acatattttg ctttggttcc gcaaaatagt
    12121 tcttgcagtt tttcaacatc ttttttcgtc ttttccaaag gtgtaaagat gtaataggca
    12181 ttttcaaata gtttagcatc tgctgcctgt atacctgatt tatgagaacc agccattgga
    12241 tgtccaccga caaaccgaag atttttctgc tcagcggatg tcacgacttg ctgcttcgta
    12301 ctgctgacat ccgtaatgat gacgtgatca gacaagttga ttctttcaag gacatcaaga
    12361 taagctaagg ttgtacggac gggaacggct aataagataa cgtctgcttg ttctgcacct
    12421 gtttcaaaat cacttgcaac gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt
    12481 tttgactgac tatcccaacc aactactatc agttctggat tggtctgacg aatacaaagt
    12541 gccatagaag caccaatcag tccaagcccc acgataaaaa catgttgtgc catcttattt
    12601 gccccctttt tagtaatgtt tcaagtagtc acggtactcc ttcactgcag ctttcaactc
    12661 aacaagcgaa tcacttggga attttgacaa tagctctgtc gctaccacag tggctacaac
    12721 attttccgca acgacgctag ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc
    12781 tttgtaaggt tcttttgtat cgatatcaac actttgaagt ggcttgtaca atgtaggaat
    12841 gggtttcatg accccgcgaa tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc
    12901 gcctagatta ttggtacgtc ggaaatagcc tctttcttct tcccatagga tctcatccat
    12961 cacttctgag cctggtaatg aaccagaagt gaatccgacc ccaaattcta cgcctttaaa
    13021 ggcattgata ctaaccacag cttgtgcgat tttggcatct agcttcgtgt cccattgtac
    13081 gtagcttcct aatccagctg gaacgcctcc aacgagcact tctacaattc ctccgatcgt
    13141 atctccattt ttctttgtct gatcgatcaa atcttttatt ctttgctcaa ctgctggatc
    13201 aacaactcgt acttctgacg cttcagaaat ttctttgatt tcttgaatcg agatatttcc
    13261 aggtatttct gcttggattc caccaagaca aatcacgtgt cctgctacga tgatgtcaag
    13321 ttcttccaga attttcttgg caattgcgcc gattgctaca cgcatggtcg tctctcttgc
    13381 agaggaccgt tcaagcacat tccgaagatc tcgatgatgg tatttcatac cgcctaccaa
    13441 atccgcgtgt cctggacgcg gacgtgcaac tcgccgcagt gattggtctt tttcaggcac
    13501 ttctgctaca gacatgacag ttgtccaatt tttccaatct ttattttcta ccactaaagt
    13561 gacaggtgaa ccaattgttt tgccgtgacg gatgccagac gtgatccgaa cacgatcttt
    13621 ttcgatcttc atccttcctc ctctgccgta accagcttgt ctgcgagcga gttcgtgatt
    13681 gatttcttct tctgacagag gtaatcctgc tggtagtcct tcaataatgg ctgttaattc
    13741 tggtccatgg gattctcccg cagtcatata acgcatctta cagtcctcct aagtaatctt
    13801 tgaatgtctc tgtgggaaca ggaacgattt ttgcttgccc aatcttttct agcaagatga
    13861 tattcaattg gcttcctctt gctttcttat catgggttat cgcgtggaat aattcatctg
    13921 gatgacttag atcgtacgta atcggcagat ggaatttttg aagtatcctt tctaaagcag
    13981 ctgtcgatcc agttggagac aagccgattt tttctgcatg agaagtgatg gctaccattc
    14041 caatagctac tccttctcca tgactgacta ccccatatcc tgctgtattt tcaattgcat
    14101 ggccaattgt atgaccaaaa tttaaaataa gtcgatttcc ttggtcaaag acatcttctt
    14161 ccactacttt tcgtttgatt tccaacgtag cagtaatgat tggaacagca tgttccagaa
    14221 gatctttttc gtctttcagc tcgttcagct gtttccaaag cgttgcatct gctatagctg
    14281 ctgatttgat gatttcagcg attccttctc gtaaacgacg taccggcaaa gtttttagaa
    14341 cctcaggatc aatcaataca ccagctggct ggctaaatgt tccgacaagg tttttcgctt
    14401 tgtttgtatt gactgctgtc ttcccgccaa tactagaatc tacttgtgca agtagcgtag
    14461 tcggaatttg aacgaaatgg atcccacgca tgaacgtcga ggccacgaat gctgctagat
    14521 cacccaccat tcctccacca agtgcaagta tcccatcgct gcgggtaaaa tcattatctg
    14581 ctaaaaaatc gtataactca gctgcttgtt ctaaagattt actggtctca cccgccggta
    14641 cgattgaaag gactggttca aaattagctt gtctcaattg gaacatgatt ttttctgcat
    14701 atagtggagc aacgtttgta tcagtgatca cagctacttt ttgaggtctc caaatcgttt
    14761 gaagccatga tccagcctgt tctagtgcgc ctttttcaat atgaatatcg tatttttggt
    14821 tatcaacatg tacagtaagc attcgtttca cttctttctt ttaaaaagct ggaattaatc
    14881 gagtgtctga atcttttcca ttgcttcttt catgatcgca acttctttca tcatgcgtga
    14941 gtaagtcttt tcattcaacg attggggacc gtctgaccaa gcatttgcag gatcaggatg
    15001 gatctctacg atcatgccat ctgctccgct tgccacaccc gcacgagcca ttggtggtac
    15061 aagttcccaa attcctgtac catgacttgg atcaacaatc acagggaaat gagttaattt
    15121 tttcatcaat gggacagcac ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg
    15181 aatcccacgt tcgataaaaa tgacaggaga tttgtcttcg actgcaatgt attctgcagc
    15241 attaagccat tcattgatcg taccggaaat tccacgtttc aatccaacag gtttacctgt
    15301 tttaccgaca gcagatagca gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat
    15361 atcgctgtac tcagccacca tatcgatatg cccttcatcc atcacttcag taatcacttt
    15421 cattccaaat tcatcagccg cctgacggat gtatttcaat ccttcttctt ctaaaccttg
    15481 gaaagcatat ggagaagtac gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc
    15541 ggctttggcc attcgcgcac attctctgat ttggtctaat ccttcgatag aacaaggacc
    15601 agccatcgtc acgaaactgc cgtcaccaat ttttactccg tctacatcca cgactgtatt
    15661 gccaggatgg aattctcgag aagtcaattt gtatgtgttt aaaatacgca cagcattttc
    15721 tactccttca tagcgtgtaa atgctccttc ttgtaaactt cgcgtatcac cttttagtcc
    15781 aatcactact cgttctgttc cttgatcgat tgagactttg aatccttctt tttcgatatg
    15841 atcgacgact ttttttattt gttcttccgt tgcttctttt ttcataatta ctatcattgt
    15901 ttttcccgct ttcttttgtt tacttatcta tcttggctga ttttctctac tttttcaagg
    15961 tggttttaca ttaattccta ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc
    16021 tttcctactt atgacccaga taatcaacaa aaaaagatgt ccctgtagga aattgcctct
    16081 acagtgaaca tcttcaacga gccttaaaaa gcgctgcaaa aatataaaat agccactata
    16141 gatgaatttc tctacgtggc tgtaagtcgt gtttaaaaat ctttagccat catagataca
    16201 aacctggttt gtttgtaccc acgaagtcat gaatacaaac ctatctaaaa tagctaaagt
    16261 aatacgtatt cagttttgta agttttctag atttcataaa aaatcgctcc aaactgatgt
    16321 aagaattgta ccta
//
LOCUS       DACDIG010000056        16257 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.056, whole genome shotgun sequence.
ACCESSION   DACDIG010000056 DACDIG010000000
VERSION     DACDIG010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.056"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="IV247_002013"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="IV247_002013"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAP8778075.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="IV247_002014"
     CDS             complement(2260..2511)
                     /locus_tag="IV247_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAP8778076.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="IV247_002015"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="IV247_002015"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAP8778077.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="IV247_002016"
     CDS             3461..3679
                     /locus_tag="IV247_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778078.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3968..4480)
                     /locus_tag="IV247_002017"
     CDS             complement(3968..4480)
                     /locus_tag="IV247_002017"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAP8778079.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="IV247_002018"
     CDS             complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="IV247_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAP8778080.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6933..7367)
                     /locus_tag="IV247_002019"
     CDS             complement(6933..7367)
                     /locus_tag="IV247_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAP8778081.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7377..8165)
                     /locus_tag="IV247_002020"
     CDS             complement(7377..8165)
                     /locus_tag="IV247_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8778082.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8162..9103)
                     /locus_tag="IV247_002021"
     CDS             complement(8162..9103)
                     /locus_tag="IV247_002021"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAP8778083.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9280..9867)
                     /locus_tag="IV247_002022"
     CDS             complement(9280..9867)
                     /locus_tag="IV247_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAP8778084.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10088..11401)
                     /gene="obgE"
                     /locus_tag="IV247_002023"
     CDS             complement(10088..11401)
                     /gene="obgE"
                     /locus_tag="IV247_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAP8778085.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11689..12699
                     /gene="gap"
                     /locus_tag="IV247_002024"
     CDS             11689..12699
                     /gene="gap"
                     /locus_tag="IV247_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP8778086.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12784..13719)
                     /locus_tag="IV247_002025"
     CDS             complement(12784..13719)
                     /locus_tag="IV247_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8778087.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            13905..14864
                     /locus_tag="IV247_002026"
     CDS             13905..14864
                     /locus_tag="IV247_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HAP8778088.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRK
                     VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(14950..16044)
                     /locus_tag="IV247_002027"
     CDS             complement(14950..16044)
                     /locus_tag="IV247_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP8778089.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT         4619 a         3565 c         2833 g         5240 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca agaagttaaa
     3721 cttttacggg ggtgtgacaa aaatcggtca catccttttt ttcgaataaa cggtgttcta
     3781 aagccagttt ttcggtatta agtcaaattg cggaaaaagt gattttttaa acttctactg
     3841 gtctttgctt gtttccattt ataaaaaaat agcgaccctc aaaagtcaga tagagaatct
     3901 gacttttgag ggtcgcttta aaatcgcttc tttaagaata acatcaagaa tagcttcttt
     3961 tttctgctta atattccatt aatgtaatga tgtcatatcc tttgattttg tcgcgtccat
     4021 ggagatccat caattcgatc aaaaatgcgc agcctacgac tacaccgcct aattgttcga
     4081 tcagttcgat cgtcgctttg attgttcctc cagtagctag caaatcgtca caaatcagca
     4141 cacgttgacc tggtttaatg gcatccttat gcaatgtcaa tgtatctgtt ccatattcta
     4201 atccataagt cacttcgatg gtttctcgag gtaacttccc ttttttgcgg actggggcaa
     4261 aaccgactcc taactcataa gcaacaggac atcctacgat gaatccacgg gcttctggcc
     4321 caactaccat atcaattcgt ttttcttttg cataatttac gatttgttta gtagcttccc
     4381 gataagcgtc accatctgcc atcagtgggg aaatatctcg aaacacaatt ccttttgacg
     4441 gatagtcggg aatactggca atataatctt ttaaatccac tctttgtttc ctcctaggtt
     4501 gttaaccatt tctttatggt aggaatatcg ctcaaaagca aaaattcctc aactttgatt
     4561 ttattgagcc gtcgctggta taatctgctc tcagtcaaag gatgactttc tgggctagcg
     4621 actttttgta aaacaccgtt ctctattgta acaaatttca attcaaaaaa cacctgtatc
     4681 ataaaaatta ataatttttc agggatacgc aaatattgag ctattgcttt tattttatga
     4741 cgtatatcaa ttcgttcttg agaatggatc aatttaaaca attttgcgta ctgttctctt
     4801 gtgcctaccc cattcaaata ggcctcatca ggtgaaatcc ccatcagata aacacgattg
     4861 aatgaataag accgaaaaac ttctttcaga tccgctaaat catctggaca atccataaca
     4921 actagagagt ttggttttct tttttcaatt attgccttta cagaatctag tccttcgaaa
     4981 acaatcgcag cttcctggat aatctcaggc acgaatttca acgtcgaaga atcaaatgct
     5041 aaataaaggc taccttccga cgttcggctc tgttcacgga aacgttttgc ccgccaatca
     5101 aatacttgca gaccacccac agaaaaatca gataccatca gctgcggttt cttacgcccg
     5161 ttccattcat tgatcgaaag ctttcctacc acatctacct gattattcag cagttcttct
     5221 tcttgagtac caaaaccaaa agcgaccgca tcaagctgac tcgatgcatc ggaaaggaca
     5281 aatttcagat gctgttggtt tgctccgatt cttttgatat tttctgcttg gacttgtcga
     5341 aataaaaact gaggcaaagg attatccgta ccaaatggcg ccaatagttt cagctcatca
     5401 attcgctcta ctgttacttc atttggcagc aatacttcat caattctcaa ctcaggccct
     5461 ctcatcaagt caatttgatg atcgactata tattggttca tcttctcctg tagtatagtg
     5521 acattttcac ttggcatcgt taaaccgact gctgcatgat gtcctccgaa gtaagtaaat
     5581 aaatctcgca tctggtcaag catttcaaaa agattcaagg cttcaatact acgtccagat
     5641 cctttagctg atccatcttc ttttaaagta aggacaagcg ttggtttacc cgtttcattc
     5701 attattttcc cagcaacaat gcccaaaacg ccttcatgcc atcctcggtt taccaataga
     5761 tggatatgat tctccttatt gaccatcgct aatgcttctt gtgttatctg ctctacgatt
     5821 gcttttcttt cttcattgat ctcattcaat ttttttgctt gaacggatgc ttcctcttca
     5881 tcaaacgtcg caagtaacga gacggcagga cgtggatcgc ccatccgacc gattgcattt
     5941 agtctgggag caatcgaaaa accgatcgtt gtttcgtctg cgtccctcat ttttacgccg
     6001 ctttcttcaa atagcgcctg aagtcctatc cgttctgaat gatgaatggc atcgattcct
     6061 aatgaaacaa ggattcgatt ctcatcagtc aatgaaacta aatcggcgat cgttcctatc
     6121 gctactaaat ccaaaaattc tgcaggcggt tcttccaata atgcagaagc tactttgaat
     6181 gctactccga ctcctgccaa atctggaaat ggatagtgcc ccgctggatg acgagggtga
     6241 acaatcgcat aagcgtctgg taaattctca ggtagttcgt gatgatctgt aatgatcacg
     6301 tctacaccag cttgctgagc gtatgctacc gcatcatttc ctgctacccc gttatcaaca
     6361 gtgatgatca actggattcc ttcttcgatc ttttcttggt ataccgattg gtttggtcca
     6421 tacccatgct caaatcgatt tggcaggaat acttctactt ctgcaccaag taattccagt
     6481 gtttctttca ttaccgttgc actggtgatc ccatctgcgt cataatcacc gtaaacgagg
     6541 attttttctc cactgattac agcttcttgg attcggctga ttgctttatc catatcgtgt
     6601 atcagatacg gatcatgtaa ttcttgttcc tttggatgta aaaatcggtt gatatccttt
     6661 tcctcatggt aacctctttg ccacagtaac ttaccgacaa atggagataa tttcagttca
     6721 cggatcattt ggagaaagga ttcagaaggc tctgtctcgc ttaacttcca atcaaaattt
     6781 gcttgtctca ctttctcact cctaacttag ttattatagc aaaggaaatc agcaaaaaaa
     6841 agcagaaaga aagaataaaa acagatcaga tccgctagac agatgttcag caggacctga
     6901 tcttttgtac gaccatttat gaaatatccc tattagtcaa aataattgaa gctttgtttg
     6961 ggcgattgag ttttttgttc ttccggcgct ttcaattgac tgatttgtgc ggttttcgct
     7021 tgtaactccg cctgataagc agcttccaac tccgcacgtt cgtttctaaa ctcgcgttgc
     7081 tgttcttctt ttgcttcttc aattttcttt tgaagttctg tttgatattc tgacatttcc
     7141 tgagtcagct gtttgatttg ctttttttgc tgccatagtg ttgccgaagc agtcaaaaat
     7201 acaatcagtg caccaatcag cgcagaaccg atgatcacta agatcaatgg cgcagaaaag
     7261 gaagtgaagc caaaacttac aggaacattt ttattgttca atacagcaaa aagaacaata
     7321 atcaaaacta aaacaaatcc aagaatcaca ttgctttgct ttttcaattc cttcacctac
     7381 tttttgttaa aaattccgct ggctaaataa tcaccaatcg ttggaaacag cgtataaaat
     7441 tttgatgcaa cttctaaaat aaacggttga ttgatttccc gttttggatg tctcatattg
     7501 cggacgatca tttttgccaa cttggcaggt tctaaaacga tttgtccgac tttttccaaa
     7561 tagcttccgc ttggatcagc cttatcgaag aaatttgtct cgattggacc tggattcact
     7621 gtcgtaacag ctacgccaag tggtttcaat tctaatcgca aagcgttgga gaagccaagg
     7681 actgcaaatt tcgtggcaga ataaaccgtt gattttgcag ttgccatttt tccagccata
     7741 gaggcgatat taatgatatg accttgtctt ctttctgcca tatcgatcgc aaatttttgt
     7801 gtcaaaacca tcattcctaa gatgttgact tcaaacatat cgtatgcttt tccaaggtcg
     7861 aatgtaagga agtcttcgaa aatcccaaaa cccgcattgt ttaccagaat atcgacgcgc
     7921 ccgacttctt ccatcacctt ttcatataat tcgtccacgc tttcaggatt gctgacgtct
     7981 accttgaaag cataagcctc tttgccgctc aagcgtctgc actcgtctct gacaccttct
     8041 acaaatgcca ttcttcgtgc gcaacttata acgattgctc cttgctttgc tgcttcataa
     8101 cagatttgtg cgcccagacc gcctgaacta cctgttacta aaacaacttt atcttctaaa
     8161 ttcatgcctc ttcctcctcg cgaaatggaa tctcaatgct atcaaaatct ttcataatcg
     8221 tcgtgttttc aaacacttct tgtgcttctt tttcaagctc taaagctgct tttcctaaat
     8281 atctcgcact gatatgagta agtagcagcc gttttgcctg cgcttctttt gcaacttcag
     8341 cagcttgatg agtcgtggaa tggaaatagt tgtgcgccat tcttgcttca tctttattaa
     8401 atgtgctttc atgtaccaga atatcgctat tttccgcaag cacaacggaa ttatgtgttt
     8461 ttctcgtatc gcctaaaata gtcacgattc gtcctttttt atctggacct acgaaatctt
     8521 ttccattgat cgtccgccca tcttctagtt ggatcgtttc accttgcttc agtttgccgt
     8581 acagtggtcc agctggaata cctaattctt ttagacgatc cacctgcaat tctcctttat
     8641 gatcatgttc aaccacacga taaccaaaac tgtcgatccc atgatttagt ttttttgtat
     8701 aaacagaaaa ttgctgatca gtaaaaatcg gggctgtttc atttagctca atgaatttca
     8761 gcggataact taaacgcgtt tgcgaaatac ctaaagagac ttttatgtat tcctctatcc
     8821 cttttggccc gtaaatctct aatggcgtat ccccaccttg aaatgaccgg ctgctgatga
     8881 gcccgggaag tccaaaaata tgatcgccgt gtaaatgtgt aatgaaaatt ttaccgattt
     8941 ttctcggacg aatagtcgtt cgcaaaatct gcatttgcgt gccttcgcca cagtcgaaca
     9001 accaaacttc attgcgctcg tctaaaagtt tcaatgctat acttgtcaca tttctatgtt
     9061 ttgcaggaac acctgcacct gttcctaaaa attgtaattc cataatctac ctgactttct
     9121 ttaagttcct ctttaatttt agggtaaaaa gaagggataa tcaaccgtaa gtcgagaatg
     9181 ggttcaaatt aaaaataacg ttctaaagcg atagcgtttt ctttgaaaat tactataaaa
     9241 acagcagcga acccgtttga gatcctctgc tgttttttat taatttgaag atccagaact
     9301 ttcattgtcg ttggaagttt gcggatgttt gtccttccaa gtcttttgtg cagcttcttt
     9361 cgcatgattt tcagcttctt tcgcatcatg cacatctttt tcgacatctt cataatccaa
     9421 gcgagtaatt tctccgccta attccatcat taccaattga ttcagtgggc gattgtcctc
     9481 ttcatcagca atcaagatca gtcctgtagc tccttcaccg attttatttc ctacaaagtc
     9541 gaacacggtc tgggcatctt ttatttcttt ggcatcttgt gtcgcaccga tcattcctcc
     9601 ggcaaaccat cctaataaaa tccctagcgg accacctaat acaccaacca acattccgat
     9661 taatccaccc gtagaagatt tgttattacc ggtaaagtcg ataaaatctt ctattttgaa
     9721 ttgatgtgtc ccattaggat catggctgac aactgccatc tgttctcctt taatcgaacg
     9781 aacagcatgt aactttttga tttctgaaaa tgcctgatat gcctggcttt ctacttgaaa
     9841 atttaaaatg attactcgtt tgctcattaa aaacgcccct ttcatacctt cttgttattt
     9901 attttacctt taattgaaaa ataagaaaag aaaagcactc tttttttcca ataaaatagg
     9961 gctgtgacaa aagtcgtatc acagctcctt tttccgaata aacggcgttc ttgaagctga
    10021 tcacttctgt cacaacctcc cttaaatctg aatctatatt ttttggtatt tgtttttatt
    10081 tgcttgccta ttcgacaaat tcaaattcaa actcaccgat acgtacaagg tcgccatctt
    10141 tcgcaccgcg tgcccgaaga gcttcatcca cacccatacc acgtaattga cgagcaaaac
    10201 gcatcactgt ttcatcatga tcgaaattcg tcatttggaa taatttttcg atcttctcac
    10261 cagataagat ccacgttgca tctgaatcac gatcgatttg gaactcagga ccttctggat
    10321 tgaatccata gtgtacagtt tcttcttcca gttcttcttc gtacaatggg aattctggcg
    10381 ttacttctaa caaatctgct gtagcattca ataacgcgtc caatccttgt cgagtcactc
    10441 cagaaattgg aaatactggg atgtcatcag caaattcatc ttctttttcc ttgttcagct
    10501 gttctttgaa tttcactaga ttttccggtg catccggcat atccattttg tttgccacga
    10561 tgatttgcgg acgctctagc aagcgtaagt tataagtaga taattctttg ttgatggcaa
    10621 gatagtcttc ataaggatct cgtccttcca tcccactcat atcgatcaca tgtaaaatta
    10681 ctcgtgttct ttcgatatga cgaaggaatt gtgtacccag cccaacacct tgcgaagctc
    10741 cttcaatcaa gcctggcaag tctgctgcgg caaaacttct gccatcactt gtagtaacca
    10801 ttcctagatt aggtacgagc gtcgtgaaat gataagcgcc gatttttgga cgagcagatg
    10861 aaataacgga tagtaaagtc gatttcccga cagatggaaa acctacaagt ccaacgtctg
    10921 ccaatacttt caattctagt tcgatttttc gctcttgtcc tggttcaccg ttttcagcaa
    10981 tttccggtgc aggattccga ggagaggcaa aacggatatt ccctcgtcca ccgcgtccac
    11041 cttttgccac aactagtgtt tgaccatttt caattaaatc tcctaaaaga gcaccagtct
    11101 ccgcatctcg gacagtggtt ccttgtggta ctttaacata ggtatcttct gaaccacgac
    11161 cgtgcatccc tttactcatc ccattttcac ctggctgcgc tttgaaatgg cgattgaacc
    11221 gaaaatccat caatgtacgt aacccttcat cgacgatcaa gatgacatcg ccgccacgac
    11281 caccgtctcc gccagccggt ccgccgtcag gaacgtattt ttcgcgacga aatgcaacca
    11341 ttccgtctcc gcccttgcca gctttcacat cgatcgttac ttgatctaaa aacatggaca
    11401 taattaagtc ctcctattat tcatttattt atttgactgc ctgaatttcc acaataaaca
    11461 aaagtcttac ttattttatc ggtgaaatct gattttgtct atcatttcag caaaaagatt
    11521 gtttaccttc ttcttttttt atcatttggt aaggtttcca accttttttc tgctaccaag
    11581 tataattttt tgacaaaata ttctttgcgt agcagaataa cagtgtatta attgtattaa
    11641 ttgaggatgc cgtttcacag cacccctaat aaaggaggaa cttttgatat gacagtaaaa
    11701 gtaggtatta acggatttgg gcgtattggc cgtctggctt tccgccgcat caaagaagtc
    11761 tcagatgata ttgaagtagt agcaatcaac gacttaacaa gtccaactat gttagcacaa
    11821 ttattgcaat ttgactctac tcatggtact tatcctggca cagttactgc aacagaagac
    11881 tcaatcgtag tggatggcga agcaactcgt gtttatgcag aacccgacgc aagcaaaatc
    11941 ccttgggcaa aagaaaacgg tgtagatatc gtactagaat gtactggttt ttatacatct
    12001 gaagaaaaag caaaagcgca tttagatgct ggtgtcaaac gagtagttat ttctgcccca
    12061 gctggtgcta tgaaaaccat cgtctataac gtcaatgatg atacattaga tgaaaacgac
    12121 aaaatcatct cagcaggttc atgtacaaca aactgtttag cacctatggc ttatttctta
    12181 aataatgaat ttgggatcga agttggtaca atgacaacag ttcatgccta cacttccaca
    12241 caaatgctct tagatggacc tgtaaaaggc ggtaatttgc gtgctgctcg ttcagctgct
    12301 gacaatacta tcccacattc tactggtgct gcgaaagcaa tcggcttagt tatccctgag
    12361 ctacaaggaa aattacaagg acacgcacaa cgtgttccag tcgttgatgg ttcattaact
    12421 gaattggttt cagtattgaa aacaaaagta actgctgatc aagtaaacga agcaatcaaa
    12481 aaacacacag ttgacaatcc ttcattcggg tatgatgacc gtcaaatcgt ttctagcgat
    12541 gtcattggaa caactcaagg ttcgatcttt gacccaacgc aaacagaagt aacaactgct
    12601 ggtgatttcc aacttgttaa aactgttgca tggtatgaca atgagtatgg atttacttgc
    12661 caaatgattc gcttactaga aaaattcgct aacttgtaat ttgatagatc aatcgataaa
    12721 agaagccttt gactggatcc agtcaaaggc ttctttcttt tcatccccta cttttaaact
    12781 cgttcaatga acatattggc ttaaaaattg ttggactact tgctgatatt tttcaggatc
    12841 tgtcttgtat gcttccgcat gctcggctcc ttttacaaca tacttttctt ttggtccctt
    12901 cgttgcacga tagaccttat ctaacatttc aaatggtaca aatgtatctt cgtctccatg
    12961 tataaagagc attgggcgct ggtttttctt caattgttcc actgcatttg cttcaccgaa
    13021 aaagtagcca gcacggactt ttgtcactag actcgttact tgcataagcg gaaatgctgg
    13081 aagactaaac atatcgtcta gctgataagc aagctcatca tgaacggaag aataaccgca
    13141 atcttcaatg attgctttca cattatctgg tagtttttct ccgctagtca tcataactgt
    13201 tgctgctccc atgctgacac cgtatagaac gatttcttgc gattttccat tgttttctag
    13261 cactttgttt atccactgta catagtcttt acgctctggc catccaaatc caatgtaatc
    13321 cccttcgctt tttccatgtc ctcttgcatc aggaacaagg acattgtaac ccatatcatg
    13381 aaacatcttt gcgtaattgg tcatcgtttc agcgtttccc atatagccat gagccacaag
    13441 agctgttttt tcacttttcg tttcagcagg aagataaatc gctgataatt tcagaccgtc
    13501 ttttgaagtg atggtccaat cgcttcggtt ttcttttgaa ctaaaccatt tatgtgcatc
    13561 cgtttctttg gatgtctgtg ttgtaccagg tgtatcttcg tctaaaaagt ctttctcaga
    13621 aggcacaaca gcataattat aaaaataatt ccctgcaaac actaacgcaa caatcagagc
    13681 taaagcgacg cctaaaagga ttttcatcca aagtttcata aatcatccat ctcatttctc
    13741 ttttttcata taaccttgaa ttagtataat caaagaatct ctactatgca atgttcattt
    13801 ttataacccg cgaattgtaa aattaagtta gaaaaataaa aaggcattta tggtacactc
    13861 aaattgtatt tccgacgaaa gaaaacaaag gagtgtaacc ataaatgacc tacaaacatc
    13921 ttaccataga cgaactgaca atgatagaat catattatct tcaacataat aagccggttg
    13981 aaatcgctaa ccgaatggga cgtgctatac aaactattta taatgtagtc aataagttca
    14041 agcaaggcaa gactgctctt gattattggc accagtataa agaaaataag aaaaaatgtg
    14101 gtagaaaagt cattcaatta cctgctcatg aagtagatta cattaaagag aaagtcactc
    14161 ttggttggac gcctgacgtc attatcgggc gaaaagaaag gtctgtttca tgcggtatga
    14221 gaacacttta tcgtttattt tctaaaggaa tatttgatat tgacacacta ccgatgaaag
    14281 gtaaaagaaa acccaatggc catcaggaaa aacggggaaa acaacagtat cagcgctcaa
    14341 tccatgatcg acctgataat tatcctgatt tcaattctga gtttggtcac cttgaaggtg
    14401 atacgatcgt tggcattcat cataaaagtg ccgtcattac tttagttgaa agattatcta
    14461 aagtcattat cacgattaaa cccaacggcc gtaaggcatt agatattgaa actgccctta
    14521 atcaatggtt ttctcgcttc cctaaaaact tctttaaatc tattacgttt gactgtggaa
    14581 aagaattttc taactggaaa gccattagta accaacatga tattgatata tattttgcgg
    14641 accctggaac accttctcaa cgcccattaa acgagaattc taacgggatt ctgcgtcgta
    14701 atggactgcc gaaatcaatg gattttagaa aagtgaatca gacatttatt tccagtgtca
    14761 gcaatcaacg taatcatatt ccaagaaaat cattgaatta cagaacacca atcgaaatat
    14821 ttttgagcta tgtacaagaa gcattttatt ctagcttaat ttgacaaatc ataacctata
    14881 aaaaaacaaa ataaaaaagc agaaataact ggctatttct agcggattat ttctgcaata
    14941 actaattatc tattttttat tttcaattaa tttaaagctt gaccatggtt tcaaaaagtc
    15001 aaggaggaat aattcgtcat cggagattct tcctacaaca ttgacacgtc cgtcgttttt
    15061 catatccttc aaggcgattt gtgtttctcc tttgtattga ccgtaccctt cattgtcaat
    15121 cagtacatcc ccatgatgca tatctcgcgt attatgaggc gggaaatctt tatctttgta
    15181 gtatacacga gtcatcgttg aacgaaggat atattctgag cgatcccctc gataactatg
    15241 aaggttatca aacaagcaga ttcgttcgtt ttctgtaata ccttcttccg tatctacttt
    15301 caatgttgga taatccgcgt taaatgcttc tgccatttct ttcaattctt cttcacttgc
    15361 gtacgcattt ccaatagaaa tatcatcaat caatcctgta aggattaaat gcttcacttg
    15421 tgtcgcaatt tcaagatctc ggtggtcttc cagcgtgcac aagccatctt gcgtaggcca
    15481 agggccaaac tcagcagatt gcgaattgac gaatgccatc gtattcaaat tatattttct
    15541 gaatttttct gaacaaaaaa caaagtgttc gtatcctaaa ccagagtatc gatgaggata
    15601 aaaattatga gaacctaata aattatccgt gtttggcgaa taactcatga tgttgtcaac
    15661 gtaattcgtg cccgaactca tattgatctc gattttgata ccaaaaggat tgcgcgtcat
    15721 ttttgcttct tctgctcctg tgaatccgat atctaagcgg accccgtagg cacccatctt
    15781 atggaaaaaa gataagtcat cgtaagaaat ctccaactgt tcaaataacg ctggattgat
    15841 atcaaccatt acttccatgc ctaggctatt cgcgtaatca actacttctt taaattctga
    15901 aagaactttt tctttatcat cattgatctg cagcaaactg gtaaaaaccc gtttataccc
    15961 atacttatga gctaaatcca agtacgcttt gtccttttca aatgttgacc gttctggata
    16021 aatcgagatt cctaattttc ccatattacc atttcctcct gcatcttttc gtttttgtat
    16081 ccacttacat tatagcaaac acaaagtaaa tggtctacac cttttctttt tcttttaaaa
    16141 taaatgataa attccaataa tttaagtagg tattttccct ctattttgtt atcattaaag
    16201 ataacaaaat agtaaattgg aaaggaacag ttatgaagaa aaataaagtt attttaa
//
LOCUS       DACDIG010000057        15654 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.057, whole genome shotgun sequence.
ACCESSION   DACDIG010000057 DACDIG010000000
VERSION     DACDIG010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15654
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.057"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            14..1141
                     /locus_tag="IV247_002028"
     CDS             14..1141
                     /locus_tag="IV247_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8778090.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1359..2501)
                     /locus_tag="IV247_002029"
     CDS             complement(1359..2501)
                     /locus_tag="IV247_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8778091.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2506..2691)
                     /locus_tag="IV247_002030"
     CDS             complement(2506..2691)
                     /locus_tag="IV247_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP8778092.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(3045..3290)
                     /locus_tag="IV247_002031"
     CDS             complement(3045..3290)
                     /locus_tag="IV247_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8778093.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(3284..3685)
                     /locus_tag="IV247_002032"
     CDS             complement(3284..3685)
                     /locus_tag="IV247_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP8778094.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(3872..5680)
                     /gene="uidA"
                     /locus_tag="IV247_002033"
     CDS             complement(3872..5680)
                     /gene="uidA"
                     /locus_tag="IV247_002033"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAP8778095.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(5703..7469)
                     /locus_tag="IV247_002034"
     CDS             complement(5703..7469)
                     /locus_tag="IV247_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HAP8778096.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            complement(7482..8276)
                     /locus_tag="IV247_002035"
     CDS             complement(7482..8276)
                     /locus_tag="IV247_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP8778097.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            complement(8286..9662)
                     /locus_tag="IV247_002036"
     CDS             complement(8286..9662)
                     /locus_tag="IV247_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8778098.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENC
                     ELLKQKHK"
     gene            complement(9782..10429)
                     /gene="eda"
                     /locus_tag="IV247_002037"
     CDS             complement(9782..10429)
                     /gene="eda"
                     /locus_tag="IV247_002037"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP8778099.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(10513..11940)
                     /gene="uxaC"
                     /locus_tag="IV247_002038"
     CDS             complement(10513..11940)
                     /gene="uxaC"
                     /locus_tag="IV247_002038"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP8778100.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(12014..13093)
                     /gene="uxuA"
                     /locus_tag="IV247_002039"
     CDS             complement(12014..13093)
                     /gene="uxuA"
                     /locus_tag="IV247_002039"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP8778101.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(13096..14727)
                     /locus_tag="IV247_002040"
     CDS             complement(13096..14727)
                     /locus_tag="IV247_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP8778102.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            15031..>15654
                     /locus_tag="IV247_002041"
     CDS             15031..>15654
                     /locus_tag="IV247_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8778103.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNE"
BASE COUNT         5067 a         3058 c         2120 g         5409 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgaagatat
       61 caatgatgat ggctcaagaa aaaaagtgga gaaagttggc ggagacaccc gaccagaggc
      121 cgaagctgct ttacgaaaag ttttatcaga tattgacgaa acaggacaat actttttagg
      181 tacggatact cgagtaaaac aataccttga tttttggatg gaggaatacg ttaaactaaa
      241 tctaaaatac aatacctatg aaaactaccg atttaccatc aaaaatcata taaacggtta
      301 tttaggaaag aaaaaactta cggatctctc ccctgctctt ttacaaaatt tcatcaatgc
      361 tgaatttaaa aagggttact cgaagaaaac aatgactatt actcactctg tccttaagaa
      421 tgcgctgaat atggcggttt atccttgggg attaatcaag caaaatccta tgctgtatgt
      481 aaaaatacca aaatatgaag cacgaccaac gactaaaaaa gatctaaaaa tcatttctct
      541 tgaggacttt gatcatatgc tagaaattac tcctgaagga catcctttct atattccttt
      601 gaatattgga ttttatacgg gaatgcgcgt tggcgaagtt tgtggtctga cgtgggatga
      661 tgtcgatttt tcaaatggaa caattactgt agagaaacaa atggtaaaga atgacggcgc
      721 atgggtatat ggtacgccaa agacaagcag ttccaatcga acgattttta ttggacaaac
      781 cttgctagca attctgaaaa aacataagaa acaacaatta gaaaatcgaa tgaagtatgg
      841 aaagctctac attgattcaa atgcagtatg tacgaaggaa gacggtgagc tagttacgcc
      901 aagtgtagtg aaatggaata ctaggaggat atcgaatgca ctctccctct cttttaactt
      961 ccattctctc agacatactc atgctacact tcttctcgaa aatggcgcaa aaatgaaaga
     1021 aatctctgaa cgattgggcc acagcagaat ttcaattacg atggatactt actcgcatgt
     1081 aacagataag atgagaaatg aaacggtcga tatcatggaa aatcttagaa agaattcttg
     1141 atttttgcca ccgaaaaaaa ccaccggtgg caaaccggtg gcaaatccat gtaaacatgg
     1201 tttatttttt acactgtttt caaacaaacc cttgctattt cttcggttcg tcctcgtcca
     1261 ttttcgatac cgacagctag cttataaccc aaggtatatc aacataaaaa aataaaaata
     1321 ggctatgaca gcagcgtgac agcaaaaaag atggaaagtt aaatatcaaa cctcgtaatt
     1381 tcatttgcga gtgcttgatt tgtttctgga tataagtgtc catatttgtc caaagtagtt
     1441 ttaatagagg catgacctag acgcttagat attgcataga tatttttatt atgttcaatt
     1501 aaaaaggcaa catgcgagtg tcttaatcca tgcatatgaa ttggttttac tcctgcaatt
     1561 tcagctagtt tttttattct gcttaaaata gttcttggag atggcggtaa tccatcaaaa
     1621 gaaaaaacca attctatatt tttcccaatt gattgttgag tttttttcca tgtttttaaa
     1681 tcttcaacgg tttgtctgtc taacgaaatt atccgaatac ttgatgtatt ttttgtttca
     1741 gcaaattcat aatcttgtct tgtacggatg taaattgact tatttacgct acatctgcca
     1801 tttattaagt caaagtcttt ccattgtaac gctaagagct cctcactcct tacacctgta
     1861 acaaaaataa aacgcatcaa acgtttataa aattcttcat aaaaatcccc gtgataaaca
     1921 ctattatata ctttcttaaa ttcatcaact gtccaaaatt ctacttgagg acgctgagtt
     1981 tttattttac ctatagtatc tacaggattt tcttcaataa tttcaagtac aattgccctt
     2041 ttaaaaacta ctctcaactt attgaatatc tgtttaatat aatttggtga taatggttgc
     2101 tcaatgccct tttttacaac atggattttc gataaaaact gttgaaattc ctgtatatga
     2161 attggtcgaa tctttacaac ctgcatttct ccaaaaaaat ccaatgcacg ttgtaaaata
     2221 tgatctgttt tcatatatgt tttttctaca gttcccaact tataccaagg aaaaaaatac
     2281 tctcgataaa aatcatgaaa cgttattcct tgcagataaa ttccttgtga atcagaaaaa
     2341 ctttgtttta actcttcgag tccccttcta gcttctcctt gagttttgaa gcctcttctg
     2401 gtcacttgaa ttctttttcc aaagcgatct tgtcctaaag ttgcagaaaa aaaccaagtg
     2461 cctctttttc tgtctttata aacattcgca attttcctca ttttactatt cctcccaaga
     2521 aatatggaga acttcttcta aaattgaaat aggaattctt tttatttttg aattacgata
     2581 aaaaaagtat cctttctcaa ctaaaagttc tttacattcc ttgaagattt tattagcata
     2641 gtactgagaa aaacctagat taatcaaatc ttttcgagta gctgttttca ttatagcacc
     2701 ttccttactt tttttcattt atttctatca gctgcttttg tcaaaagcac ataggaattt
     2761 cacctctccc caagtttacg gcgagcctta cggaagtatc attatcccaa ctttaaatca
     2821 tcgcttttta ttgactataa aaattatttt agaatattta tcgctccttc tttacagcaa
     2881 gaaatcatgg cgcaactaaa atatgctcct taaagtgtta acgtactgat agaatctatt
     2941 taattttcaa tgaacaatag gttttctacc taaaagaatc tactaattcc ttaactatta
     3001 gaagattctt ttaaacagaa aaagtggaaa ggggattttt ttaattagtt aattttaaat
     3061 tttaagatcg cttctattaa tttaatttct agctgactta tcatatattc atctacatag
     3121 gtaacgaatt gacctacctc gttaaatcca tttgtcaaac ataacttagc catataagaa
     3181 tgataatgtt tgagtatcat cttgatcgaa attggatctc cttctagagc agataagata
     3241 atggatagtg gaactaatag gtactgccgc tttttagatt tatctaccat aggtcatacc
     3301 ccattttcaa ttgttcaact gccttctttc gttgatacca gataccactt gtgctcatat
     3361 tgagaatttt accgatttca gcatcattaa aacctgcaaa ataataaagt aggataatct
     3421 ttcttcttat ctccgataac tgatcaattg ctgctgataa cttttgatct gaaattaata
     3481 tttccattcc aaataagaaa tagacatctt gttcatcaaa atgagtttgg gaatacctgc
     3541 cttcatttga atctgccaat ggttcctcat tatcctttat gttagcgttt cgttttcgaa
     3601 tagacttagc ttcgtttcgt aatacttttt tacagtaggc atcaaattgg tgttgtatag
     3661 atatgtcatt gtaatgttgt tgcatgataa tatgctcctt tttccccctt tccactaata
     3721 caacaatcag aaagcccctt tttttcgaaa aaactagaaa aaaaacataa aaatatttat
     3781 cattctttaa acagaaaatc tttctataaa aaacaaaaaa aaacgattag gctaggctaa
     3841 tcgttttttg aacattctta aaaaatccaa attatttttt ataactgaaa ttgggtattg
     3901 atgaccatcg tttctgtaga tagtaagcaa tcattttagg ttttctatct ctagtaaaga
     3961 ttcctttctt atttccttgc actcttaaaa tcccttgact tgttgcaaaa tctgcaaaat
     4021 tccacacttg ttcaccaaca aaattttcac atgtatctat tacttcgtga tttgcttcgt
     4081 aataagccac ttgatactct tctgtaaaca ttgttggtac tgtatcatga aatccagcta
     4141 ctgtatctgc tccatattct gtaaacatta ttggcgtatc aggacatctt tccatccatc
     4201 catctagctc ttttttcaac agatattttg catgtgccag atcccctccc gaaacatacc
     4261 aaccataata acgatttaaa cataaaacat cgacaatatc tcctacctta caaacatctg
     4321 gcgttgattt tagataagta acgattgtta caggtctttt ctgcggatca agttctttag
     4381 ttaattcaac taaaggttca aaatattctt tagcaccttc ggaatccgaa tctggttcat
     4441 ttgcaataga ccacattaca acacttgggt ggttcttgtc gcgttctatc aattccttga
     4501 tcaccgtttt atgagaattt tctgttcgta tctctttcca cgtattatgt ttttccacat
     4561 tatcatctaa taaagtggcc atgaaattta aatgtaaccc tactgcaggt acttcatcaa
     4621 taactacaat accttctcga tctgctaatc gcataatttc ttctgagtaa ggataatgtg
     4681 atgttctaaa agaattagca cctatccatt tcattaaatt aaaatcttta atatttacag
     4741 cctcattcaa accacgtcca ttaacatacg aatcctcatg cttaccaaat cctttaaaat
     4801 aaaaaggttt attattaatc aaaaatttgc cattggtaac ctgaacactg cgaataccaa
     4861 attcttctgt ataggtatcg atcactttat tatcatcaat taaactaatt ttcatactat
     4921 ataaataagc gtctagaggt tcccaaaaaa ttggattctt aagctgaatc attcctttct
     4981 ttccttcagc ttcagctaca agttgagcct cttcgtcata tatcgctatt aagatttttc
     5041 cggtccattc tgtatcgata ttgaattcta cacttgcatc caaatcagtt attcttgtat
     5101 taatagtaat atcacttata tagtctttcg gagttgtata taacttaaca ggtctatgaa
     5161 tacctgcata attgaagaaa tcaaaattaa ctaagttttc cttttttata tccccatttt
     5221 cctgtactgt ttccttcata atccccacag gtaaagtact atcgtcaata atattgttta
     5281 cagcaactgt aattcgatta ttcccgtctt ttacaaaccg attaatttca gactcaaatg
     5341 gtgtaaaccc acctttgtgt tgagtaataa actttccatt caaaaaaact tttgcttcat
     5401 gtgttgcaga tccaaaacga agaactaatc tttcttctaa taatctttta ggaagagtca
     5461 cttctttttc ataccacacc catccaacat gatttctaat gttctctgtt tcaaataaat
     5521 cattataaga agagggaact gaaatcggat aactctcttt ttcatttatt ggttctaaag
     5581 caagttcttc ctgtaagtca tcatttgtat ctaatttaaa tctccagaga ccgcttaagt
     5641 caattacttg cctcgtctct gtcataatgg gatacaacat ataaaccact cctatctaaa
     5701 tattaaataa atgtatcctt taaaccacaa taagcatcta ttggacttac tcccttaaat
     5761 tcagaaagac ccattagttt gtccaccaat atttctaaag tttcttgttt tccatcataa
     5821 gtattaatat aagtctttac ttgaggtaca tcagccaaat gaaacggaca ctgtacagat
     5881 acaaaaattg ttggaatttc atgaatgtaa aacggtatat ccggagttcc tttacttgct
     5941 ggccagacaa ttcgttgaac tgtgcctgga ttaacattag ctatattaat gatcaagtca
     6001 tattttttag ttaaattttt aattggtctc ttagcagcat atattccagc tatattttta
     6061 attttttctt cttgcgataa tcctttcaat tcttcttcag gtgaaatgta tttttcaact
     6121 tcaaatcctt ttgctgacaa aatctgtttc aataaatcaa tggcttgagg tctatttcca
     6181 gcaactactt caccaaaccc accttttacc ccatgtacat ctactagtaa aatcctttta
     6241 tatttttctg gtacaatagg ccaaatatct tgtttatctt taactaatgt aattgcttgg
     6301 tcagccacct ctttaaaaat tgatttattt tcaattaaac caattttttc cagagcttct
     6361 tgcttaggta ataaaatttg ttcttttgct tttttgtgta atcccaattt agcttttaac
     6421 cctaaaattc tttttaaggc atctaataac cgatcatcag tgataattcc tttacgatag
     6481 ccttccatca tataattaaa gtcttcatct ggatcattaa aaaatagaaa tagatcacat
     6541 ccagctgcaa tagaagtggg caataattct gatcgtttca ttgccccagt cattcctaac
     6601 atatgactag catcagtaac tactaatcca ttaaatccga gttttcctct aagtaagtca
     6661 gtgattaatt cttttgataa agttgcagga agtaaatctt catctttcag atcaggatta
     6721 aaatgttttg aataagctgg catatggata tgacctgcca taattgaagg taatccagct
     6781 tctattaagc ctgagtatac ttttccaaaa gaattatccc attcttccgt acctaaacta
     6841 ttaatactaa aagatagatg ctgatctcgt tcgtcaatac cgtcaccagg gaaatgttta
     6901 gctgcaggca aaattccact ctcctgaata cctttcatat aagctaaact aagttctaaa
     6961 actttatcag ggttttgtcc aaaagatcga ctagaaatga taggatttct ccaatttcta
     7021 ttaatatcta caataggagc aaaactccag ttacatccaa tagccgatgc ttcaatccca
     7081 gaaactctcc ctaattcata agcatatttc gcatcatttg ttgcaccaat ttttacttct
     7141 acaccaactt cagtaccatc tgtacaagct ccgttaccac ctgcttccgt attagcagca
     7201 attaatagag gaattttact ttctgtttgt aagatatagt tttgatcata tacctcatca
     7261 gcagttcctg gattatatcg tacagctcca atatggtact gattgaccat attactcaag
     7321 taatttttat cacgactaga tcccatatta acaaacaatt gacctatttt ttcttccaaa
     7381 gtcatagaat caatcgtctc ttcaatccaa gaaatagcat cttcatctaa attatatggt
     7441 ttttttcgta aatctacttt tatagtcatt ttctttctcc tctacttatt agtaagattt
     7501 ttccaaaaag tatctatata tttttgttca atatctacat aatctcccga taaacattta
     7561 aataaaacat cagttcttaa gttttcccat gattcttttt ctttattgac caaaatagga
     7621 taaaagaaaa ctttattttt atttgcagct tctagatcac caggagaatc accaatcatt
     7681 aatatcttat ccaaatcaca accattttca attacacttc gaataatatc ttcttttttt
     7741 cctttatctt gacaacacag ttcatcaact aatgataata attcatgtct ttcccattct
     7801 tcttgaacag cttccttatt agctgagctt acaataatta tcttcccata ttttttcaga
     7861 gcttttatag tatctctagc acttaaaaag ggtttatcta atccttttgc ttgactaatc
     7921 tgttgattga cttcttccga ccattttaaa gcttttaata aatcctctga ctgagaatat
     7981 tctatttccc tcttcaaaga tggattagac aattcttttg ttagagatac ccagtgcttt
     8041 aatcgattaa tcgatgttac atcatttttt tctaatgttt taactaaacc taaaaatctg
     8101 tttattccac gagtctctga atataaatta atattattcc aatcctgtag gaatttttct
     8161 ctatgatgaa catcaaaaat ctctgcagca attggtccga aaaagagatt gtgtttataa
     8221 gtcatcgtat ccataacaca gccatctgaa tcaatacaaa atatccaatt attcatattt
     8281 ctctcctatt tatgtttttg ttttaaaagc tcgcaatttt ctaagacttt atctttagat
     8341 aatggataaa ggaaaaataa aattaatcct acaatcagaa aacaaattga tggaattaat
     8401 gttgaagcgt tatataactt ttctaaaaca tctcccgttt gtacatttgc taaagagtca
     8461 tagcctataa tagaaagagt ccaacctccg acgcctccag ctagagcttg acctattttt
     8521 ctagcaaaag aatataccgc atatacggtc ccatcttctc tttgatcaga ctttacttct
     8581 tgatcatcaa taacatcagt aatatttgcc caaataacag tattaaacac acccaaaccc
     8641 atatagccaa ccgaactaag aattaagaat acatacatat tactagcttt tatcaaaaat
     8701 aagatcaaat atactattgc tgaaaagaac attcctactg atgccagttc tttttttcca
     8761 tattttttac ttaaaaacaa agttaaaggc gaaacaacta ttaaaaccaa aataggattg
     8821 ataaaattca ttaaaactat tccctgtgca tcattatagt aatcaggaaa tacataatta
     8881 ttcatactca taattaataa ttgagcaagg agtaagaaaa tagccgcacc aattatagaa
     8941 agtaatgccc tatttttaac taaccctttt aaagatttta acaatgaaac tttgtattga
     9001 gattggtcaa ttcttactcg ttcagtagtt agtttataac atattaaata acaaataagt
     9061 gcacatatcg aaaatattcc cgcaacaatt gtaaaagttg atccaccacg aactatttga
     9121 gcgcctgaat catctcttgt ataaataaat aatggtgtta atacaccaat gaccaaactt
     9181 gctaaagtag ctccaacatt tcggaatgtc gatagcattg ttctttcttc tgtaatagaa
     9241 gtaatagctg atgccattga tccataagga atatttatag atgtgtaaaa aatactcccc
     9301 cataatatat acgtaacata catgtatact accttaacgc tcatagaagc tgaaatcata
     9361 gaagtttgat acattaaaaa acttgctaaa gcgactggac cactcattct taaaatccac
     9421 ggtttaaact taccctgctt agttgctttc gatctatcaa caatacgccc catagttaca
     9481 tcagtaactg catcgataaa tcttgctact aaaaataatg ttccaacggc agctccgctt
     9541 atccccaata cttttgtata aaagatcatt aaaaaagaac tagcaaaaat gaaagtaaaa
     9601 tcgtttccaa aatctccaaa catatatcct attttgtctc ttataccaaa ttttctttcc
     9661 atacaattct ctcccttctg ttgttaagcg ttttcattct gatgaaagta cgtactatac
     9721 tctgaagcgt aaagagtcag tatttatttt ttacatttca gagtattatt tgttttttct
     9781 cctaataatt ctttatctca taaaacttat ctatatattt actagctact cgacttactt
     9841 cttcaaaatc acctttttca acagaagcca ataaattccc acctacacca actgcaacaa
     9901 ctcctacttc aaaccaatct tctaagttct ctatacttac tccgccagta ggcattatat
     9961 ttacgtgtgg taatggagct tgataggctt ttacaatgga tggaccaaaa gcagaacctg
    10021 ggaatagttt aatgatatct acgccagcag taagtgcctc ctttatctca gtaattgtca
    10081 tacaacctgg taaataagga atttgatata aattacaaat ctcagctgtt tgtcgatcaa
    10141 aagatggact aacgatatat tctgccccag ccataattgc gatacgagca gtaaacgcat
    10201 ctaaaaccgt accggctccg attattacat ctttttgatc agcatatttt ttagcaattt
    10261 ctttgattat ttcttcagca ttaggaactg taaaagttag ttcaatccct ttaattccac
    10321 ccgcaataat tgaatttatt gctttaatgg cctcatcctt atttttccca cgtacaaccg
    10381 caatgactcc agccgcttct aacttactta aaatttctac ttttttcata tctatctcct
    10441 tcctttatgg atacatactt tgtctagctg tactaactta caaagaataa atttaagctt
    10501 ttatttcaag tattattttc tacttccgat ttcaaaaaaa ttaaaataat tatacgcatt
    10561 acgataacaa atattctcca ctatctgact aagatacatt tcatcatctg gaattcggcc
    10621 tgctttaacc cactcgctta gtaattgaca taatactcga cgaaaatact catggcgagg
    10681 atagcttaga aaacttcgag aatcagtcaa catacctgta aaattcgaca acaaactttg
    10741 ttgtgccatc gtagtcaact gttgacgcat tccttcagct gtatcattga accaccaagc
    10801 acatcccaat tggatttttt gaatcatacc cccttggaag ctttgcattg ctgttgctag
    10861 tggaagccaa tcagttggat ttaatgaata taaaattgtt tttggtaagc acccactctt
    10921 ttcagcggta tcgaacaatg ctacaacatt ttgtgtcaaa ctagcttgat cccccatcga
    10981 atcaccaccg acatctactc caatttgttc ctttaatata cttgaattat ttcttaaagc
    11041 atttaaatgc atttgcatcg tccaaccata tcttgtatac tcagacatca aatgtaattg
    11101 aatacctgta acaaattgtg caatttctga taaatggaaa tccgtttttc ctgctaaagc
    11161 ttttattaac agattgtcga gtattttttc atctgctatt tcataaaaat aagtattcaa
    11221 accgtgatct gctaatcgac agccaatttg atgaaaatga tccactcgtt ggctcaatgc
    11281 ttgcttcaac ccattaaagt ctttgattgg tatagccgct gtttgactaa gttttaaaat
    11341 atagttcgaa aagcctgatg atttgatact ccagacctta tctggacgga aagtaggtaa
    11401 taccttgaaa gaattatcct gtttagaaag ctcagtatga taccacaagt catctgtagg
    11461 atcatccgtt gtacagatgg ctttcacatc catcatctcc aacaattctt tcgggcgata
    11521 ggaatccgtt tgaatttttt cgttagcacg tcgccaaatt tctggtgcat tcttctcaga
    11581 aattgtcaaa tcaatatcaa agtaacgttg taattccaaa tgagaccatt caaaaatagg
    11641 attacctggc gccttctcca ctgtttttac aaaagctaaa aatttttcat aatcatcacc
    11701 tggaccactg atcaactctt cttctatacc attagctcgc atcaatcgcc atttataatg
    11761 atctccagct cctttatcat ttaaccatac ctgagtcaaa ttcttgtatt gcttattttc
    11821 tagcaaatat gctgggtcta aatgacaatg atagtcgata attggttggt gttttgcaaa
    11881 tttatgaaaa agtcttttag ctatttcatt ctctaataaa aagttcttat ctaaaaacat
    11941 atttaaacac tcctttcctt ccgttagttt ttagaagaga aaatttttcc tctaaataaa
    12001 ctcccttata taattattta ctcgccccct tttcaagcgc ttcccaaata ccatttaaat
    12061 aaactgcacc tagagctcgg tcatataatc cataaccagg tctaccggtt tctccccaaa
    12121 tcatccgacc atgatctgga cgaatatagc cattaaatcc attatcgtac attgttttta
    12181 atatcttata catatctaga gaaccatatt ctgatttatg cggcgcttcg taaaaatcga
    12241 gtccattgtc acttaaatat ttgatattac gaacatgtgc aaaaggaatc cgatcccgtt
    12301 gacaaaattc agatagaatt tcatagacat cattatctgg tcgttctgca atacttccag
    12361 tacataatgt aataccatta gaaggagaat ccactacatt acacaaccaa tctaaatcgt
    12421 cacgattttt aacaatacga ggtaatccga aaatggaata tggtggatca tctgggtgaa
    12481 ctgccatttt aatcccaact tcttcacatg tcgggataat tgctttgaga aaatatgcaa
    12541 agttctctcg taatttctct tcatcaactt ctttataggc ttctaataat tgcttaattt
    12601 gtgataaccg atgaggttcc caacccggca attcaaaatt attagtagaa ttttctactt
    12661 ctctaataat ttcctccgga tcagtaggga tattttttga aatataggct aacgtagaag
    12721 acccatcatc taaacgataa tccaaatccg attttaccca atcaaatact ggcataaaat
    12781 tataacagat aacctcaatc ccaaattctg ccaaattgcg aatcgtctgt ttataattct
    12841 caatgtattg atctcgtgtt ggtaaaccaa ttttgatatc atcatgaaca ttaacactct
    12901 caataacttt caaggtaagt ccattggctt cgacttcatc ttttaaagat ttaattcgtt
    12961 ctctcggcca aacctctccg gctggtatat ccattaatgt tccaacaatt cctttcattg
    13021 ctgggatctg acgaatttct tgtagtgaga ttttttcttc atttgcccca taccatctaa
    13081 aagtcatttc catagttact ttcctccaac ttctgttata tatttattga ttgttttacg
    13141 aactgcttga ggacctacta gcatttcgca aaagattttt tcagttttct ttcctacccc
    13201 aacttgatat aaatctaaac caaagatttc tttattttct aaaatttcct gtaccgcaca
    13261 atgaatcttt gaaggatctt gttcacctaa tttcaatact gataatttat cctgcaactc
    13321 taccaataac ggatcaggac ttggcacaaa attttcccct tgatcatcga ctgccagtaa
    13381 ataacgtaac caagcagcaa tagcgatagg aataaatgtt agattttctg catttccgtc
    13441 tttttcgaca tactttttaa ttgtttcacc atagcgaatc gctacttttt gagaagtatc
    13501 tgttgcaatt cgttgtggtg tatctggaat atagggatta ggcaaacgtt tattaattac
    13561 ttcatcaata aattgttttg gatcaataat gcctggattt tcaactactg gtaaaccttc
    13621 taaataacct attcctttga ttaaaccaac caaatcatca tcctgcatct ctgctgaaat
    13681 tgattgatat cctagcagac aacctgttac tgccaaagca gtatgaagtg gattcaagct
    13741 tgtcgtaacc ttcatgatgt ctgctttatc aaccgtctct ctatctgtca taatcacacc
    13801 agccttttcc aatttaggcc ggccatttgg aaaactatct tcaataacta aataatgaac
    13861 tacctcagta ttagtgaaag cagcaatatt ggtatgtttt tttgtgtgaa tgatatctaa
    13921 atcctcgagc cccatcatca atagatcttc ctttacattc tctgaaggat ttggcgtaat
    13981 tcgatcaatc atactccaag gaaaagtcac tttttcttta tcctttaaat aagcaataaa
    14041 tttttcagaa acatatcctt ctttttgcca agcttctgca atcgtaaaaa tactatcttg
    14101 aaaacgttga ccattctgtg agaaattatc agtagaaacc atcgcaatcg gcaactctcc
    14161 agcattaaat ctgctatata acaaggatgt tacaatactc atcgtatgtt gaggattgct
    14221 aggtccagat ttgatatcag ctttgactac cgaaaagaat tgccctttag aatcttttaa
    14281 actgtaacct ttttcagtaa tagtaaacgt tgtaaactgt aatttaggat cttcaaaata
    14341 tttaaccact ctttgatagt ccgctatatt cttcggattg caatataaac tatcagccgt
    14401 tactgccaaa agtctttttt tgagttcacc atctccactc ataacaattt ccaaaatgtc
    14461 attgttatag tcatgataag ctttttccac tacttcttca tcaaatgttt cacacacaac
    14521 tactcctgtt tctaattcat tttgatctgc taagcattga gcaatttctg catgaaatcc
    14581 tcgataaaga tttcctccac caaaatgaag ccatactgga tgttgctggc cttcttgctt
    14641 aacttctttg acatcaaaac acggcagtag aatctgcatt tcattgaatt cttcgataga
    14701 attttgccaa tttttaatcg aaaccataat taacctcctc aaactgatat gttagttttg
    14761 atacatttta tattttaaaa cccattgtaa ccgttgtcaa tataaaaagg gaataaactt
    14821 gcttttttct accatatcag aatgattgta ttttttattc agaaaaataa aatactcctt
    14881 ctttttggac tgattttatc gaaacttatt ttttcttttt ttcatgtatt cttttatttt
    14941 tataactagt gtattacttc aaaatttgat attatatggc atgaacagaa ttaaaatatc
    15001 agttaaaata aaagttaagg agcattgtgt atgacaatta aaactagtct ccaaaatcaa
    15061 gcttaccaat ctattcgccg acaaattatc tatgctgact tagaacctgg aaaaaaagta
    15121 tcagaaaaaa aattagaaga aacactttct attggtcgta caccaatacg tgaagcactg
    15181 attcaactcc gacaacaagg tcttgttgat acaattcctc aaagcggaaa ttatatctca
    15241 ttaattgatt tagattcggc caaaaatgct cgatttgtca gagaacattt ggaacgacaa
    15301 attatgttag agtgttgtgc caaaatgaat aaccaaaaaa aacggatttt agaaactata
    15361 atttcagaac aagaaaaagc tgttagtcaa aaaaatgctc gtagtttttt tcataatgat
    15421 aatttattcc ataaagcttg ttttgaaatt gcagagcgag aagaagtatg gcaatggtta
    15481 gaaaataaca atacacactt agaacgattt cgatggctac gagtaaaaac ggaaggatta
    15541 aagtgggaaa aaattatgga acaacattat caactatttg aggcattaac aaaacaagat
    15601 ccagaagaag ctaactttct aacatcgttg catcttcatt taatgcttaa tgag
//
LOCUS       DACDIG010000058        15313 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.058, whole genome shotgun sequence.
ACCESSION   DACDIG010000058 DACDIG010000000
VERSION     DACDIG010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.058"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            278..787
                     /locus_tag="IV247_002042"
     CDS             278..787
                     /locus_tag="IV247_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8778104.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="IV247_002043"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="IV247_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8778105.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLTPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="IV247_002044"
     CDS             2322..3302
                     /locus_tag="IV247_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8778106.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="IV247_002045"
     CDS             3424..4842
                     /locus_tag="IV247_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAP8778107.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="IV247_002046"
     CDS             4913..6382
                     /locus_tag="IV247_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8778108.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="IV247_002047"
     CDS             6392..6859
                     /locus_tag="IV247_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP8778109.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /locus_tag="IV247_002048"
     CDS             6895..7767
                     /locus_tag="IV247_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8778110.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7849..10383
                     /locus_tag="IV247_002049"
     CDS             7849..10383
                     /locus_tag="IV247_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8778111.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            10539..11312
                     /locus_tag="IV247_002050"
     CDS             10539..11312
                     /locus_tag="IV247_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8778112.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11305..12096
                     /locus_tag="IV247_002051"
     CDS             11305..12096
                     /locus_tag="IV247_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8778113.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            12106..12597
                     /locus_tag="IV247_002052"
     CDS             12106..12597
                     /locus_tag="IV247_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8778114.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12615..13025
                     /locus_tag="IV247_002053"
     CDS             12615..13025
                     /locus_tag="IV247_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8778115.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            13029..14429
                     /locus_tag="IV247_002054"
     CDS             13029..14429
                     /locus_tag="IV247_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAP8778116.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14452..14826
                     /locus_tag="IV247_002055"
     CDS             14452..14826
                     /locus_tag="IV247_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP8778117.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         5315 a         2156 c         3060 g         4782 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga cccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttt caattagtaa ttgaagggac gcctaaaagt gaaaaaaaaa
     7861 atgaaagatg aagtattaga gtatatcaaa aataatttaa agtattacga tttttctgct
     7921 caagatattg caatgaaatt ttgtattaaa agaaatgtcg cttcccacta tttgaatcag
     7981 ttattttcag atggaaaatt actaaagaat gattctgtta gaccagtaat gttcaagtat
     8041 aatcaacaaa agccaaaaga ctgcttttct aagtttattg gagcagacat atctttgaaa
     8101 tccactatag ataaatgcaa agctactgta atgtatcccc ctaatggcct cccattaata
     8161 ataaaaggaa acagtggcgt tggaaaaagt tttttagcaa gtttgatcta tcagtatgct
     8221 cttgatagaa aagtaattca taatgacgca aaatttgttg ttgtgaattg tgcagattat
     8281 gcaaataatc cagagctatt gtcagcagtt ttatttggat ataaaaaagg agcctttact
     8341 ggggctgaaa aagatactgc agggatgcta tcttctgcta atggaggata ccttttttta
     8401 gatgaggttc acaatctaag tgcagaaaac caagagaaat tgtttcttct tatggattct
     8461 caaaagtatc gtaaacttgg tgaaagtgtg caatgggagt atgcaaatgt tcgccttatc
     8521 ttagccacaa cagaagataa aaatacctct ttgttagcaa cttttagaag acgcatccca
     8581 gctgaaatca ctctgccaga ttacaataat agaagtacat ctgaaaaaat tcaattatta
     8641 tttgaatttc ttaaagatga agcaattaca attgattcaa aaatattttg ttcgattgaa
     8701 ctttttactg atcttttatc gaaaacattt gaaggaaatg ttggagaatt gaggaatgaa
     8761 atcaagcttc tttgtgcaga aggttattta ggaaataaga gaaagtcttc aatttattta
     8821 ggggacaaac tagacagcgg tttttggata gatccagaaa ttagtataga tttaaaaaaa
     8881 atgtttctta aatcactttc tgaaattgat ttgattgaac tttttcaaaa tcacatttca
     8941 attgaagaaa gcatgagtca attaagagat agaatactta aagagtgtgc aggttcttct
     9001 gagtataatt atttagaaag tgacgaattt atagcattaa gaaactatgt tgttaataaa
     9061 atatcaccaa ttattgattc gacaggtcta tgtttattag atgaattctt ggcggatatc
     9121 agtctattta taatgtttat tgatgtaatt gaagaaacat caagaaattt tttgctgtct
     9181 aagttcagaa aatttcgtat gaaaaatgat aaacaaaaat tattggcaaa tgagatttgg
     9241 agtagtttag aagtcgaaga aagtaaacaa gaacttttgt tgaaatggat actttttctt
     9301 gtaaagaagt tctatgtgaa aataccagaa acccattgtc tgatagttat gcacggtaaa
     9361 ataacagctt ctgcgatcgc aagggaaaca aataagttgc tgaatacata tgtttatgag
     9421 gctttcgata tgccaattga aggtgagact agtgacttaa ttaacctaat caacaatttt
     9481 tgtaaaagta tagatacgac gaatggactc attctgctag ttgatatggg atcattggaa
     9541 caaatgtatg aaaaaattga atccaatgta ataggtgatt tggtaatttt aaacaatgtt
     9601 tctacagctt tagcgattga atgtgggatt caagtttgcc aaaaaaagcc aattagtcat
     9661 ttttaccaga tggattttga ttcgtttcaa gttaaagttc agtattataa aggattatca
     9721 caaaagaaaa atgtaatagt atcttgtctc tctggtgagg ggatttctga aaaagttaag
     9781 gatattttaa aacggtatct cgataatcaa gtggagattt tgatttttga ttttaacgca
     9841 ttaaagaaaa tagcaaagga aaaagatact atgattttta agaatacaat ttgtgttctt
     9901 tcaaccactg aattttacat tcaaggaatc gattgtttaa atttagaaaa tctaattaat
     9961 gggaatcaaa ccttagaaaa attaaataag tatatggact cggatagctg tgagttctgc
    10021 ttaaatgaat tagttaaact ctttactatt gaaggcgcta gtactaagct tcgcttttta
    10081 gattccaaaa aagtattcaa tgagatagaa aaagtactat atttgtatga aaaatattac
    10141 gatgtcaaaa tcccaagttt tctgagaatt aatctatttc tacatctttc tggaatgatt
    10201 gaacgaatta tgattggtga tggaataaca aatgaacatt tgaaagaggg agcgcaacta
    10261 tttgatactt ttttaaatgt atcaaaagat tttttttcag aaatcaaaga tatttactgt
    10321 attgaaatac ccaaaggaga gtatgagtta atctatacaa tttttgagca gactattttc
    10381 taaatagcat actagcgtct aaaaaaatga aagggtgtcc aaagtatatt tagacgctca
    10441 tttaattaat atttactgta ttttcccgct ttattggata atgttagttg gcacagtaat
    10501 tgcttatata aaagtataag aagaaaggag agaacaaaat ggcgttttgg caaacatttt
    10561 tagttgtgtt gtatggattt ttcattaact gggataagga agggcccaaa atcggaacct
    10621 cccaacctgt cgttgcaggt ttcttgaccg gacttattat gggagatatt caaactggac
    10681 tttatatcgg tgggactttg caattaatga cactaggtat tactagtttt ggtggtgcat
    10741 cagtaccgga ttatcaaaca gcagcattga tcggtactta tttagcaatc tcaactaacc
    10801 aggatgcttc tataggaata actttagcaa taccggtagc aatgttaatt gtacaactag
    10861 atgttctaaa gtggtcaaca aacatttttt ttcaacaaaa ggcagaaaaa ttcgcacaaa
    10921 aagaagatta tcaaaaatta gagtggatgc aatatttggc atcaattaat acaagctttg
    10981 tatctggaat tcctgttttg ttaactgtga tattaggccc aacgttagtg ggaagtgcaa
    11041 tcaattatat tcctgaatgg gttaccggtg gtttaaaggt agcaggggga ctattacctg
    11101 ctgttgggat aggaatgcta cttaaatatt tacctacaaa agattacttt gcctatttgt
    11161 tagttggttt cgttttggca gtttatttcc aaataccaat tttagggatt tcgttacttg
    11221 gcgctgcaat cgcactaatc gtatataaaa atagatcaaa taatactcaa gtagcaccag
    11281 ctgcaacagg aggttttgac gaagatgaat aagaaactga caaaaatcga caaacaagta
    11341 attttccggt ggattctcaa tggtgcaact tcattaaatt atgaaaaaat ggaagggttg
    11401 ggttatgcgt attctatgct tccttttatc aaggacacat ataaagatga tcctgaagga
    11461 atgaaacaat ccgttttagc tcacctacaa ttttttaaca ctacgccgta tacagcgcct
    11521 tacattattg gtatgaatgt tggaattgaa aaagaagaaa aaaataagtc tttggaagca
    11581 gtaagtgctc tgaaaacagg tctaatgggt cctttagcgg gaattggaga tagtgtattt
    11641 gtagttattc catggactat ttttggagca attgcagcta atatggcatt ggaaggtagt
    11701 tttgttggaa ttattttgtg gatactggca tcaattttat taaggattac tgcttatcct
    11761 ttataccttg ctgggaaaga atcgggtgcg agcctactaa ctcaaattga aacaaaatta
    11821 aagtctatta ctgatgcagt atcaatttta gggttaatgg tggtcggtgc actcattgcc
    11881 acagttgtaa aagtgaatgt tggctttgaa tttaaacaag gcgacttaac aatgaccgga
    11941 aatgaaatct ttgatcaagt tatgcctggt ttattacctg catgtgtagt agcaattgtg
    12001 tattatttgt taggacgaaa ggttaaacca gtttatgtaa ttctattaat cttagtagtt
    12061 tctattgcgt taagagcgtt aaatattttg aagtaggtgt tgaatatgga tgaaaaaatg
    12121 aaaggtatta tacatgttcg tattgatgat cgaatgattc atggacaagt agcaacacaa
    12181 tggacaaatc aattgagtgc taacagaatt atggttatta ataatgaagt agccaatgat
    12241 gacgtaaaaa aagctgtagt tcgcctcgct gcaccaccta atgttagaac ttcaataatt
    12301 acaagggaag tagcagttaa aaatattctt tctggtaaat atgaaggaca aaaagttctt
    12361 attgttgcag tttctccaat cgatgttaaa tttttgattg ataacggttt accaataaca
    12421 agtgtaaacg taggaaattt atcaagaagg aatggaacag aacgcatccg tccgacgata
    12481 aatattactg aagaagagag agaggctttt aaagaactaa ttagtgatgg agttgaagtg
    12541 acagtgatac aaactcctag agattcaaaa gaatttttaa aggattttat ttattaagaa
    12601 cagaggatga gtggatggag tttataatta catctcatgg aaatattgcc attggatttg
    12661 tagatacatt aaaaatgttt tttggcgata atttaaattg tcatgtggta acacttggag
    12721 atgcaggcat tgaagaattc cgtgaaaaca ttgaaaaaat agtttctcaa gtaaaaaatc
    12781 aagaagttat ggtatttgct gacttgattg gtggaacacc tttcaacgaa tttgctaaaa
    12841 gaagtgaagt atttcaaaat gggtttcaac ttcttggtgg catgaattta ggaattttga
    12901 ttgaggctgt caatttgaga ttacaaggaa aaaaaagcga tgatatcatc gaaaatctaa
    12961 gaaatatgaa tacaattgcg tgtcttgcag atatgcaaag acatactaac gaagaggatg
    13021 agtgataaat gcaaaaaatt ttttacaagc ctaaagattt ttggtttggg gattgcatgc
    13081 ctttttataa agatggaact ttttatctat ttcatcaacg agatacgaga aatcctaaac
    13141 ctttaactga tccttttggg tggtcactag ttacaaccac tgattttgtg aaatttgaag
    13201 aatacgggga agtaattcat cgtggtggca acgatgacat tgatcaatac atttacgctg
    13261 gaagtattgt ccaaggaatc aatgagtatt tagctttgta tactggacat aatcgcaaag
    13321 ctaagttgga tggaaaaact tcagaagtgt taatgattgc aagttctaaa gatttaattc
    13381 attggaaaaa ggatggcgaa gcagcaaaac ttgttcctca agaaggatat gataagaatg
    13441 attggcgcga tccttttgca atttttgatc aagatcatgg aaaatacgtt ttgatactag
    13501 gagctagaaa accagcaagt aagaaaaagc cgacggggag attggtctat tttgaatccg
    13561 aagatttaaa agattggaat ttcaaaggag actactgggc taataatgaa tttaacatga
    13621 ttgaaatgcc tgatatattt gaaattgatg gaacgtggta tttacttttt tcagagtatt
    13681 gtgaagataa aaagactaaa tatcgagtcg gagcaggatt attttcagat tggaaatcac
    13741 cagtagacga agctttcgac ggaaaagcct attatgcagc aagaacagtc ggaactaatg
    13801 aaactggccg ttttctaact ggctgggtgg caacaaaaga aggggagaac gatcaaaata
    13861 actgggattg gggcggtgca cttgttccac atcaaattat tcaaaagaca gacaaaactc
    13921 ttggtgttaa actgcctgaa aaaataattg agaattttaa agaaatcact ccattttcaa
    13981 gttttaagtt agagaaagaa tttggaaaaa aagaaagagt aattgttcct aaaatcgaag
    14041 attattttct atttgaaaca actttgacta ttacatcgtt aacaaaggag tttgctttaa
    14101 aattatttgt agatagtgat actcaagaag gatacgaatt tcgtatttca ttgattaata
    14161 atcagcttac tttcggaaaa actccgaact atccttggcc tcaaatgttt gataaggggc
    14221 tgcaacgacc actatttttg gaaataggca aagaatatga actaaagtta attgttgatg
    14281 atacgatagc tgttttatat attgataatg ttgctttaag tactagaatg tatgataaag
    14341 tcggaacagc attaagtatg actgttacgg agggagcaat caaggtagat aaactagact
    14401 attcaataaa ttatgaatgt ggattttaaa acaattgaag ggatgatgta agtgaaatta
    14461 aatggtttac aacatattgg aattccaaca aaagattttg gagcttcaaa aatcttttat
    14521 gaatccttag ggtttagtct gattaatgaa gaaatgaatg tgggaagtaa agtcggcttt
    14581 ttcgagttga atggaacgat aatagaaata tgggaagata aaactactga gtgtagagga
    14641 gccattgatc atctagcatt agatacggat gacatagatg aggtattcaa ttcaattcaa
    14701 gatttaggat atgaattatt agataaaaat attatggaat taccgttctg ggaaaatgga
    14761 atccgttact ttaatttcta cggaccaaat catgagatta tagagataag ccaaatgaag
    14821 aaataaatag ataacgaagt gctctaagtt ggtatgaacg aaaagtactt cattttttgg
    14881 tatcagcatt tcaatagttt aatgagaatt tactatttca aattaattca aagtgacagt
    14941 attttttact tatttggttt caaattttgt aatccttcct atttattgga atacactcca
    15001 cattttgtat acaagtcata tcatgtttta gcaaagctct taccaagcaa agttataatg
    15061 atgttttcca aagtttgatc tttcgatgga ggctaacaca tattttttgc aggacagcca
    15121 aaattagatg tatagaatag agtctgtgag gcgtatagac agtttattgt ctgtatgcct
    15181 ttttttattt tgaaaaattt ttagtatttt tcgaaaaatg ctcactccta gagtaagcgc
    15241 cccatagaac agtaccttac taacaaatgc acctcaagat atacttgagc tatcaagaag
    15301 tctggaggtt ttc
//
LOCUS       DACDIG010000059        15284 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.059, whole genome shotgun sequence.
ACCESSION   DACDIG010000059 DACDIG010000000
VERSION     DACDIG010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15284
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.059"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(41..407)
                     /gene="ssrA"
                     /locus_tag="IV247_002056"
     tmRNA           complement(41..407)
                     /gene="ssrA"
                     /locus_tag="IV247_002056"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(578..1351)
                     /locus_tag="IV247_002057"
     CDS             complement(578..1351)
                     /locus_tag="IV247_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8778118.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1495..1845
                     /locus_tag="IV247_002058"
     CDS             1495..1845
                     /locus_tag="IV247_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAP8778119.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1826..2542
                     /locus_tag="IV247_002059"
     CDS             1826..2542
                     /locus_tag="IV247_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8778120.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2542..3990
                     /locus_tag="IV247_002060"
     CDS             2542..3990
                     /locus_tag="IV247_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8778121.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4060..4569)
                     /locus_tag="IV247_002061"
     CDS             complement(4060..4569)
                     /locus_tag="IV247_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778122.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4559..5284)
                     /locus_tag="IV247_002062"
     CDS             complement(4559..5284)
                     /locus_tag="IV247_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778123.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(5507..7627)
                     /locus_tag="IV247_002063"
     CDS             complement(5507..7627)
                     /locus_tag="IV247_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP8778124.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            7857..9035
                     /locus_tag="IV247_002064"
     CDS             7857..9035
                     /locus_tag="IV247_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAP8778125.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            9051..9809
                     /gene="aroD"
                     /locus_tag="IV247_002065"
     CDS             9051..9809
                     /gene="aroD"
                     /locus_tag="IV247_002065"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAP8778126.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFFSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            9832..10317
                     /gene="ybaK"
                     /locus_tag="IV247_002066"
     CDS             9832..10317
                     /gene="ybaK"
                     /locus_tag="IV247_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAP8778127.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(10388..11641)
                     /locus_tag="IV247_002067"
     CDS             complement(10388..11641)
                     /locus_tag="IV247_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8778128.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            11758..13107
                     /gene="gdhA"
                     /locus_tag="IV247_002068"
     CDS             11758..13107
                     /gene="gdhA"
                     /locus_tag="IV247_002068"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAP8778129.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            13219..14565
                     /locus_tag="IV247_002069"
     CDS             13219..14565
                     /locus_tag="IV247_002069"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAP8778130.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(14873..15259)
                     /locus_tag="IV247_002070"
     CDS             complement(14873..15259)
                     /locus_tag="IV247_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP8778131.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         4988 a         2737 c         3104 g         4455 t
ORIGIN      
        1 ataaggattt aagcatctag cgaaatctgc tgaaatctca caatggagac ggcgggagtc
       61 gaacccgcgt ccaaacacat cgccacttaa aaatctacgt tcatagtcaa ctcatttaag
      121 gattcacttt acagcttgcc gagtgacagg cattctgtat cgctagtctg atgttctctt
      181 tttcaactta cagacggaaa gttgaaacgt atcccactaa agttaggacc cttactcgag
      241 cacatgggcg atgccgagag gatctacgct aactgttttt aggcagctaa agcgtaagag
      301 ttgttttcgt ttttagcagt tatatttaac tgtaacgttt ttacgtagac gtaacctacg
      361 aaacgcaatt caagctcgac tgtgcctgtc gaatccgtaa cgtccccgtt agctaacaag
      421 ggttacgtgt atttttcata caaaattagt atagcatata ttgggataaa ttcaataaaa
      481 aagcgaatca tatcccatta tttatataat aactgcccct ttcttaagaa aaaataagcg
      541 attttcttaa gaaaagaggt agcttcaaaa aaagatctta aatcagatca aaatacttca
      601 atgcgtggac aattccgcct tctgtatttt tctttgtgat aaaggtagaa agttctttta
      661 atccgtcata ggcgttgccc atagcgattt tgttgtcgca agcttccaac aaggcaaaat
      721 cattcggacc atcgccaaat ccaaaagtag gaactttttc cagctgaagt tctttttgca
      781 gacgtttgac acctgcccct tttgagattc ctttcttcac tgtatcgatc gagtaaggac
      841 cattccgata aaaagttagc tcaggaaaat gttcgtgata gtattcgtca cctgattgag
      901 ataagatcag caacatattt actgtgtttt ctttaaagcc taaaggatca atttccggaa
      961 catctgaatg aatgaagtcg taagcattga tcattgtttg gctatgaccg gtacaccaga
     1021 ttttctgatc gttatagtaa gaaagttcat gaccgttcaa ctgtacatga tcatacattt
     1081 tttgacattc ttctgaagaa aatgtttgag aatagatttc ttcgccgtct actcgaatga
     1141 aagaaccatt catggtgacc gcagaagtaa tgcccgcatc ttccatgata gagtggattt
     1201 ctgcttctgt acgtcctgta gcgatgacgg ggagaacatt gttttctttt aatgcgttca
     1261 tagcttttgc cacttcgggt gtgatttttg atttcgcatc tagtaatgtt ccatcaagat
     1321 caaagaaagt gattgcacga taatttgaca caaataaaac tcctttttta ttggaataag
     1381 tttatcttaa caaagaatga acaaaagaca aacctttcgt gaagaattcc tgaacatatt
     1441 ggttcagtca acaatttaga gtatactata agaagagaat ttaggaggga aagaatgatt
     1501 tttttcagta ttggtatctt attcttaata attggcagtc tttttcggat tctgccctca
     1561 aaaggaaatc tgccttttta tgggtatcat tcgcctcttg ccgcaaaaac agacgctcat
     1621 tggcggctgg ctcaaaagac aagcggaaac tggttttttc ttatgggctt actgatggca
     1681 ttgatcggct attatttaaa gacatctggg catacgaatt attttctgat tgaaatgctt
     1741 ctgctagtat ttccaattat gcctattttt attatgacag aaaagaaatt acagaaatat
     1801 gacctggaaa ctggagggaa cgataatgaa tattttaatg attgaagata atcaatctgt
     1861 ttctgaaatg atgcagatgt ttttcttaaa tgaaggttgg gaagcaactt tcaagtatga
     1921 tggaaaagaa gggctcgatg cctttttaga aaacccacaa cattgggata tgattacact
     1981 agacctgaat ttgcccacac tggacggtat gctagtagga agagagatcc gcaaagtttc
     2041 aagtactgtg ccgattatca tgctgacagc tagagattct gaaagcgatc aggtgatcgg
     2101 cttagagatg ggggcagatg attatgtcac aaaacctttc agtcctctga cgcttattgc
     2161 tcgaatgaaa gctttgcatc gccgtagtga gttggcagaa gcaagagaag aagcagtagt
     2221 ttcagatgat cattttgata tcgagactag tcatttcaag atgaatacga aaacacgaga
     2281 agcgtatctg gacaaccggc caattgaagg actgacgcct aaagagttcg atctattgtt
     2341 tactttagca aaaaaaccac gtcaagtatt ctctcgggag cagttgcttg aattagtctg
     2401 ggattatcag tactttggtg atgaacggac agtggacgcc catatcaaga aattacgaca
     2461 aaaaatcgaa aaggtcggac cacaagtcat ccaaactgtt tggggagtcg gctacaaatt
     2521 cgatgattct ggtgttgcct aatgcgttat ttgtatcagc aactcttagc tttctgcgga
     2581 atggtcgcgt tgatcttatt gatcgttgga gtttctttta cacaactgac aaaacaaacg
     2641 attgaagaaa acaattacca gcaactgttt ggatatgctg aatcagttga aaagacaacg
     2701 caaacctatg ccgatacgct cccgcagttt agtcaagatc aggcgtttca aaatgcattg
     2761 ctgctgacag agcaagtact gacagaacaa aatgtgaact ttatatttat cgataaaaac
     2821 gaacgtgtca tttatccgac taatacagct ttactgaatt ttaccttaac agctgatcaa
     2881 tggaaaaatc tgaggaatgg acgtcaagaa aaattcacat cgaataaaaa tattttagga
     2941 caagcacagg cgacctctta cgcacttgtg ccatttaatt tgaatcatga attctatgga
     3001 gcacttgtag ttagtcaacc tgcgcgaaat atcgataaca gcgttcgtcc aattacactg
     3061 aacctgttca aaggatttat tttttccagt atcgttgcag ttattgccag ctatgctttt
     3121 gcagcattcc aagttaagcg tatcaatcgg ttaaggaatg cgacgaaaga agtgacgaat
     3181 ggtaattttg atgtacagct ccccgtacat gacaaagatg aatttgatga gttagcagat
     3241 gatttcaata aaatgacgaa ttcattgaaa gaatctcaag cagaaattga agaacaagaa
     3301 aatcgtcgaa gacaatttat ggcagatgct tctcacgaga tgagaacacc tcttacgaca
     3361 atcaacggac ttctggaagg gttagaatac aatgcaatcc ctgaaaatca gcgagaaaac
     3421 gcgattaaat tgatgaaaaa tgaaacagag cgtctgatcc gtttggtgaa tgaaaacttg
     3481 gattatgaaa aaatccgtac gaatcaaatc agtatggtca ttaaaaaatt caatggaaca
     3541 gaaacattga aaaatattgt tgcacagtta gaagctaaag cagaagcagc aggagataca
     3601 ttgacattga aagccgttga tgatattgat gtttacgctg attatgaccg atttgtccaa
     3661 atcatggtca atattattca aaacgcgata cagtttactg aaaacggcca aatcacgatt
     3721 actttagaaa aaggctatct tgaaacgatc atcacgattg aagatacggg aattggaatg
     3781 tctgaacaac aaatgaaaag tatctgggat cgttattata aagtagatcc ttcaagaaaa
     3841 aatacaaaat atggtgaatc aggattaggt ttaccaatcg tgcagcaact tgtacgcttg
     3901 cataaaggaa aattagaagt agaaagcgaa ctaggaaaag gaacaaaatt cgctgttcgt
     3961 ttgcctgacg tagaaatcaa aaaagagtaa aaaacaagcg gtataaaaga tcatgaatcg
     4021 atcttctata ccgcttgttt ttactataaa atgattgatt tagcccgcgg gctctaaacg
     4081 atcaatataa ggactttgag caatagtagc atcatgagta aagagtaaga taccttgatt
     4141 ttcttcatga gctaaataag aaagcaagtg gagtagctct tggatctcca atacactaag
     4201 ttcatcaaat gtatcttcaa taacaatatt gtttcgcttg gaaagcaagg catgaagtaa
     4261 ttgcagcttt actttttcaa aagacgtcaa ctcagctgcc gcacaattaa gcaaggaagg
     4321 agagatatca agtttttctt gataggccaa acgatcttgt ttgatttctt tttccggtac
     4381 atccaacagg acatggtctt taagattcag ataaggaaac atcggccaat ttttttggat
     4441 gatcgcaaaa ttatccagtt ttgcaacaag aacctctttt ttcaagtgct ccaatatttt
     4501 atgctgtttt tcgagaggac acaaaataac ggtcaagtgt tcagcatgaa tcaaatattc
     4561 aggtttcatt acgtccaggc ctcctacttt gcaaatgatg gataaaaaaa gtgaaaggga
     4621 atagactaag aatgagacag catccactga gcccaataag agtttgggca aatcccttaa
     4681 gtatatctgt gaaaaaaaga tcattttgga aatcgatggt aaaaaaagtc tgttgatcca
     4741 tcggaattgt aagtccgctt accgaagtca agatatcacg gatatctggc agttcagtag
     4801 tctttaagac aagaaaattg atcctttgaa gaagcgcttc ataaactgac tggaagctta
     4861 cgatccacaa taaaaccaag atacaacaaa gaaagagagg aaagaacagc tgccagaaaa
     4921 taaaaaggaa taatttggtt ttagaaagac cagtcaggtg ccaattgatc aattcctgct
     4981 tgttttgcct gactgaatag taaaagaaaa agataaaaag gaaggcaaat agaaaaaata
     5041 aactgaatat gccttttata taaaatcggc tagctaattc taaagccgga agaagctctg
     5101 tagatgcaga agcgccaata aatgaaagcc gatcttggac ttgattggct aaagccgatt
     5161 gcaaatggta aagagtcagg catgctatca ataaaaaagc agaaaagctt gaaaataaac
     5221 aatagagtat tgtctgcttt ttgtaataaa ttaatcgagc agtagcatag cggattattt
     5281 tcatactttt cacctctata aaaagtatag aaaatgaata tgaataaagt atgaaattcc
     5341 tttagtgaaa tcacgaatta aaaccagaaa atattttgta aaaagcaaga ggttgtgaag
     5401 aagctgccct attctattac cgtagatgca agcttttgaa cactgtttat tcgaaaagag
     5461 gagcagcgac aataattttg tcgctgctcc ttttaaattt attttatcat tcggaagaac
     5521 ttgtggtcgt tccgtgaagt tcttggtacg tttgcgtttt ataaagatca acagttgatt
     5581 gtccgttgtg ttcactaaag acactagttg acttgtcacc aagagatttt tcagcttctt
     5641 ttaatttctc taagccattc ttgtagtcat aatcactggt atcaattggg tctaatccac
     5701 tattcgtata gaaccgaaga agatcaccat tattgatttg gtcagaaaca gctaactgtt
     5761 tatcaacttt ttctttcagt gaatcaattt cattctgtac ttcctcagta ggttcagtaa
     5821 tagcttcacc agtttcgtta ctatagatcg taccaccgta gtaagtatat tttggtgtaa
     5881 caaaatcacc atcacggaaa gaaacaagct gttcatgatc tttcgagaat agatcttgtc
     5941 ctaactgaat ataatttttt gtatctacac ctagtaagtg aagtaatgta ggcaaggcat
     6001 caacttgtcc gccgtaagtg tgattgattc cgccctgtgt ttgtccaggg atatggatca
     6061 tataagggac acgctgcatt tgtgcattgt cgtagtttgt ccaagtagaa cttgtttttc
     6121 caactaattc agctagattt ttatttcttg aattcgagac accgtaatgg tcaccgtaaa
     6181 ggacgatgac tgaattatca tacaagccgc tttctttcaa ataattgaag aattcttgta
     6241 cggcggtatc taaataattg gctgtcgcaa agtaaccgtt gatcgtttca tcatttgtat
     6301 tggctaacgg gaatcctgct tcgtcattgg tgaattttga ataaggataa tggttagata
     6361 cagcaatgaa ttttgaataa aatggttgtt gcagacgttc caagtattgt acggattgtt
     6421 ggaaaaatgg tttatcatgc aacccgtatt ggaacgaatt gtcatcatta acatcataat
     6481 aagaagaatc aaagaaataa tcatatccta aattcttgta tgtctcgttt cgattccaga
     6541 atgtaccagc gttcccatgg aataccgcac taatgtaacc ttgtgtttga cttaaaatat
     6601 caggtgcagc ttggaaagta ttttttccgc ctaattgtgt gaacaaggag ccttgattca
     6661 agccaaacaa tgaattttcc atcagcgttt ccgcatcgct tgtttttcct gcttttactt
     6721 gatggaaaaa gttgtcaaaa ctgaacgtgc tgttgctgtg gtaaagacta ttgatgaaag
     6781 gtgtaacttc atgctggaga ccattctcat ctgtcaattt ataatcgatc aggaattgct
     6841 ggaagctttc taaatggata taaatcacat tttttcctct agcgagcccg aacaaatcgt
     6901 catttggtgc tgcataatgt tcttttacat aattttctac ttctttcata tcattgggac
     6961 ttgcttctgc ccgcacttgg tttgtctggt aagtttgaac accgtcataa accatgaaag
     7021 cattcaagcc aagatatttg accaaataat cacgagagaa tgttcgagtc agcaattctg
     7081 gacgatcagc ttccgccata aaaagatttc ctgagaaaat cataactgct aatgtagaga
     7141 tcgcaaaagc cattcttgct cggatcgggc gagggtccaa cttgattttt tttgcaatca
     7201 gcagaacagt caagataata aaatcgatcc aatagatcac atcatatggg cgaaacagtc
     7261 gaatggcact ttctcccaga ccactcgcta ctttcccagc acctaaaatc gtgttgatcg
     7321 taatgaagtc cgtgaattct cgataataag tgacattcga aaaaacaagc aatgtcaaaa
     7381 gaaaataaat gaccatcatc gtgatataag caactctagg gcgtctgata taaagagcaa
     7441 ttccaagtaa taacaatgta gttgcaatag ggttgataaa caagataaag tattgaatgc
     7501 cactttcgat tccgagatgg aaatcaacta aataggcaaa aatcgttttc aaccaaagta
     7561 aaacgactag taaagaaaaa aatccgagcc tcttattcaa aaaggcaggt gtttttatac
     7621 atttcaagat aatcttcctt tctgcacttc ttaatcacag aagtgtcctg aaaccatgtt
     7681 acacagctca cttattctca tttattttac tagtatgaca atatagtgtc aattgaaacc
     7741 agaagtttat gctgtttcat taaggttttc tttaagcttc atgaaaaaat gagcagtaag
     7801 acaagtccta tgttttttgt tatacttggg aagagaaagc taaagcaggt gaaggaatga
     7861 aagtaaaagt aaaaaaaaga gcaagcaatc gattgaaaaa aggatatcca ctgatccaaa
     7921 aagaagacct tgctcatccc caaaaagaaa ccacgcaatg ggtggaatta gttgattcgc
     7981 aaaaccaatt tatcgcaaaa ggatatctag gaaagcagaa taagggaatc ggttgggtat
     8041 tgagccagca gaacatccca tttgatcaat ctttttttga agaatttttt gtgaagtcaa
     8101 aagaaaaaag aactttcttt ttttcagatg attctactac cgcttttcgt atattcaatg
     8161 gagaaggaga cgggatcggc ggtctgattg ttgattatta tgcaggttat gctgtttttt
     8221 cttggtataa cgaaacgtta tatgcacatc gtgaattatt gattcaagct tttcgtgaaa
     8281 tctacccgga aatcatagga ggctatgaga aaattcgatt tgaatcagta aatctgcctg
     8341 agagccagtt catttacgga aaagaggcac cggaaccctt gatcgtaaaa gaaaacggag
     8401 taaaatttgc cgtttattta aatgaaggat taatgactgg gatcttttta gaccaaaaag
     8461 aagtacgggg atcattagca gaaggtttcg cttctgggaa aacagttttg aacatgttta
     8521 gttataccgg cgccttttct gttgctgccg caatgggagg ggcaatcgaa acaacaagtg
     8581 tcgatctagc aaaacgcagt ttaaaaaaaa cacaggaaca gtttgaagtg aatggtttgg
     8641 atgtcgatca gcaaaaaatc attgttatgg acgtattcga gtatttcaaa tacgccaaaa
     8701 gaaaacaatt tttatatgac atgattgtat tggatccacc aagttttgcc agaaataaga
     8761 aaaaagtctt tcgcgtagca aaagattatg gggaactggt caaggatagt ctggatattt
     8821 tagcagaaga cgggatactt attgcgtcta caaatgctgc gaatgtctcg atagaacaat
     8881 tccaaacaat gattgaagag gaattccatg ccaaaaatgt ttcttttgaa caacttgctt
     8941 tatatcgtct gccaaaagat ttccagacga cggatacctt tttagaaggc aattatctga
     9001 aagtgtttgt ttatcaaatt aaaaataacc attagaagga gcgatgcaga atggttcggg
     9061 taaagggatt gaatatagga agcggtagac caaagatttg tgcgcctctt atcggggccg
     9121 tccgagagga agtattacgt gaagcagttc ttgcacgtga agcaggggcc gatttagtcg
     9181 aatggcgagt cgatcattat cgtgaagttt tttcttttga tgaagtagca gaaacattgg
     9241 ttcttatcca tgagatgctt gatggactgc cgttattatt tacgtttcgt accttagctg
     9301 aaggcggaga acaagacata tcgatccgtc agtatcgcga cctttatgaa catgtgatac
     9361 acacaggatt agtagatatg gtcgatatcg agttgttcaa agtagaaagt ctaggaaaaa
     9421 gactgataga ggaaatcaag tctttaaaca ttcctttaat tatcagtagt catgatttta
     9481 aagaaacacc agcagaccct gtcttgcttt accgattgaa tgttatggaa cattttggag
     9541 ccgatatcgg aaaactggca gtaactccca ataatgaacg agatgttttg cggttgatgg
     9601 agttgactag acgggcaaat gcttttttct ccatgcctat tattacgatg tccatgggaa
     9661 atcttggtaa aatttctcgt ctggttggcg gaacgacggg ttccgttatg acatttggtt
     9721 ccttaagcag cgattctact tctgctccag gacaattacc ggtcaaagca ttgaagcaaa
     9781 ttattcaatt attaaatgaa ccgttgtaga acaagtttga ggtgatattt tttggcaaag
     9841 aaaaaaatag taaaaacgaa tgcaatccgt atggtcgaac aaaaaaagat tccctataca
     9901 gagcacgaat atgagtggga cgaaagccac ctaagtgcgt catcggtcgc tgaacagctt
     9961 ccagaaagcc aatctcgtat atttaaaaca ctggttgctg tgggcaatgt gacggggcct
    10021 cttgttgctg tgataccggg agaagccgaa ttgaatctaa aaaaattagc aaaagtttca
    10081 gggaataaaa aagtagaaat gctccattta aaagatttgg aagcaacgac cggatacata
    10141 cgtggtggct gctcgcctat aggtatgaaa aaactatttc ctacttattt agatcaaatt
    10201 gcagaaagct atgaacaaat cattgtctcg gctggcagaa gagggctaca gatggagtta
    10261 gcacctcaag atatctgtgc attaacgagc ggacaatttg cagatataaa acaataaatc
    10321 atacgtataa aagaaagaag gaggccggga tgttgtttgt ccagtctcct tctttctttt
    10381 ttatacatta tgctctttgc ccggcattca ttcctgctac atgccctgta gcgaaagccg
    10441 ctgtgatatt aaagccgcca gtataaccat tgacatccag taattctccc gcaaaataca
    10501 gaccatcaat gattttactc tccaatgttt taggataaat ttcctttaga gaaacccctc
    10561 cgccagttac aaatgatttc tcaataggaa aagttttgtt gattgatagc ttaaactctt
    10621 tgcatagttg aacaaaatct tgaatttgtt tttgagaggc ttgttgtcct gtcagatgat
    10681 ccatttcaag ccgctcaaga aagaaaacta acaatcgctc aggcaatata ccatgccaag
    10741 cattgaccaa gttctttttg gttgctttac ttttttctgt caatatatga ataagctctt
    10801 gtttggtttg tgtagggaaa caatccagag aaagtgtcac tggttctccc gttttctcta
    10861 attctttatt gacaaaactt gaacaacgca gtgcagccgg accagaaata ccgaaatggg
    10921 tgaaaagtaa atccatcgta tgttcagtca atacacgacc ttttttattt aaaacgcgta
    10981 aagtgatgtc ttgtaaagaa agcccttgaa gtgttttctc ctgaatatac ggttcatccg
    11041 atatcaaggg ggactctgtc gcataaagag gcgtcaccgt gtgacccact cgcttcacga
    11101 tcttgtaccc atctccagta gaaccagtag atggataagt ccgtccacca gttgttaaga
    11161 taatggcagg agcatggaac tcttcgaatt ccgtgcgtat accgtaaatt ttatgttctt
    11221 tgtggatcaa tttctctact cgagtagaga atagaagcgt cacatctagt tctttcagac
    11281 gattgagcaa tgcttcgata atcgtttttg atttatttgt tacaggaaac atccgtccat
    11341 gatcctcttc ttttaaatgt acgccttgtg attcaaagaa gttcatgata tcgaaattat
    11401 tccattgcgc aaacgtacta tataaaaact ttccatttcc aggaatatgt gcaatcaaat
    11461 catcaacagg acgattgttc gtaacattgc accgaccacc acctgtcatc agtagttttt
    11521 ttcctgcttt tttgtttttt tcgatcaaga gtacgcgtgc gccttgttca gctgcactga
    11581 tcgctgccat catcccgctt gttccagcgc cgactacgat cacatcatac agtttttcca
    11641 ttccctcacc tcaaggaaag tgtagcatat ctgaagaaag aaaacagaaa aaaatgtttt
    11701 gagccaaaag agaaaatgag ctaaaatgag ggaagataag caaaggagcg tggaacgatg
    11761 acaaacgcaa cagaatatgt tcaagcaatt caagaaaagc tacatcaaaa ggatcaaggg
    11821 caagaagaat ttttacaagc aatcgatgaa ttcatgccca cggttatgtc ttttttggac
    11881 actcatcccg aatatatcga aaagaatatc ttagaacttt taacggaacc agaaagagtt
    11941 attcaatttc gagtgccttg gcaagatgat gaaggaaact ggcgagtaaa tagagggtac
    12001 cgtatccaat acaactcagc tatcggccct tataaaggtg gattgcgctt ccatcctagt
    12061 gtcaatctaa gtatcttgaa atttttggct tttgaacaga ttttcaagaa tagtttaacc
    12121 ggtctgccaa tcggtggagg aaaaggcgga agtgacttcg atcctaaagg aaaatctgat
    12181 aatgagatca tgcgtttttg tcaaagtttc atgctggaat tgtcaaaaca tatcggccca
    12241 tcgattgatg ttcctgcggg agatatcggt gtaggagcta gagaaatcgg ctatttgttc
    12301 ggcgaatata aacgattgaa aaaatatgat actggtgtgt tgacaggtaa acctttggat
    12361 ttttggggaa gtaagatacg tacggaagct actggttatg gattagtgta ttatgtcaaa
    12421 catctattga acgaagaaaa agattcattt aatcaaaaaa cagtttttgt ttccggaagc
    12481 gggaatgtag ccatttatgc aatcgaaaaa gtgcaagagc taggcgggaa agttgttaca
    12541 tgttcagatt ctagcgggta catctatgat ccagaaggaa tagatgtgaa actattgaaa
    12601 gaagtcaaag aaatcaatag acaacgtttg acggaatacg caaaccaacg tccttctgct
    12661 atttatcatg ctggagaatc tgtttggaca ttgaaagaaa aagcggatat cgctttgcca
    12721 tgtgctactc aaaatgaaat agacgaaagc ttggccgaaa tcttagtaga aaatgggatc
    12781 aaaatcgtag cagaaggcgc taatatgcca tgtacagtaa aagctgtttc tgttcttcat
    12841 gaagcaggcg tgtggtattg tccaggaaaa gctgctaatg ctggaggagt agcagtatct
    12901 gctttagaaa tgagtcagaa cgtccagcga ttgacttggg aaagagagca agtggatgag
    12961 cagctggatc aaatcatgga aacgatttac catacgtgtc gccaaacagc agccgagtac
    13021 ggcgacgaaa aaaatctgct tcttggagca aatgttgctg gatttgaaaa agttgccaaa
    13081 gcaatggcag cagaaggttt agtttgaaaa atgaaacttt cttggaaata ttgtcagcat
    13141 tttgtttatt tgcttgaaaa ataataaaaa aaaacgtaaa gtattacctg tagagtaaac
    13201 ctataaggag gagaacaaat ggcacacatt cattttgact attcaaaagt agcaccgttt
    13261 gtcaacgaac aagagttagg gtacatgcaa agccaagtaa cagcagccca caacgaatta
    13321 cgggaaggta ctggcgcagg aagcgacttc cgcggctgga tcgatttacc aacgaactat
    13381 gacaaagaag aatttgcacg tatcaaagaa gcagctaaaa aaatccaatc tgattctgaa
    13441 gtattagttg ttatcggtat cggtggttct tatttaggcg ctcgtgcagc gatcgacttc
    13501 ttgaatcaca cattcttcaa tttattagat agcaacacaa gaaaagcacc acaaatcttc
    13561 tttgccggaa attcaatcag ctcaacatat attgctgact tgatcgaagt aatcggtgat
    13621 cgcgactttt cagtaaacgt tatttcaaaa tctggaacaa ctactgaacc agcgattgcg
    13681 ttccgtgtat tcaaagagct tcttgttaag aaatatggac aagaagaagc aaacaaacgt
    13741 atctacgcta caacagataa agcaaaaggt gcagttaaag tagaagcaga tgcagaaggt
    13801 tgggaaactt tcgtgattcc tgatgacatc ggcggacgtt tctctgtatt aactgctgtt
    13861 ggtttgttac caatcgctgt tagtggtgca gatatcgatg cattgatgca aggtgccgct
    13921 gatgcaagca aagcatactc aagcgacaaa ctagaagaaa acgaagctta ccaatacgca
    13981 gcaatgcgca acattcttta ccgtaaaggc aaagtaactg agttgttgat caactatgaa
    14041 ccaggaatgc aatacttctc agaatggtgg aaacaattgt atggtgaatc tgaaggaaaa
    14101 gaccaaaaag gcatctatcc ttcaagcgca aacttctcaa ctgatttgca ctctttagga
    14161 caattcatcc aagaaggacg tcgcaatatt tttgaaacag ttgtgaaggt tgaaaaacct
    14221 cgtaaatcaa tcacaattcc tgaacaagca gaagatttag acggtctagg ctacttgcaa
    14281 ggaaaagaag tggacttcgt aaatacaaaa gcatttgaag gaacattgct tgctcataca
    14341 gacggtgacg tgcctaactt gctagtcaaa attccagaaa tggatgctta tacattgggt
    14401 tatacaatgt acttctttga aattgctgta ggtatttcag gttacttgaa tggaatcaac
    14461 ccattcgacc aaccaggtgt agaagcttac aagaaaaaca tgttcgcact tctaggcaaa
    14521 ccaggctttg aagatcttgc taaagaattg aacgaacgcc tgtaagataa gggtttttcg
    14581 ctagatttaa ataatcagtg atagaaacat gttcgcttta aaatgaaaac tggtacccat
    14641 ttaggtactc aaacatactg catcgtttat tcagaataag gggctgtgac aagacgtttg
    14701 tcacagtccc ttgtttgtat agaaatcgat tgattgcctt gctctttagt ttgttttagg
    14761 actttaagaa cagtgaggct gacttcgaag gttaattctg tttttttgtt ttctagaatc
    14821 agtaagcaaa aaagcactca cttcgagtgc ttttttgcga tatattctta gcttaagata
    14881 ggcctaattt ttcttttaca gatgagggta ccttatcgct ggtaattact tttttctcta
    14941 tgatccgctt ttcgcttgct ttcaactgca agatcgttcc ttcggggtaa gcagaaccat
    15001 tggcagagtc accagaagta atgacatcga aatctaactg tcttttttca ccgttttcag
    15061 cataagcatc tacgttgtat ttaaaacctc ttccaattac ttccccatta tctgctttga
    15121 tatcagtttc tgcaggcatt ggagctttga ctaccgcata ataatcttta cctacataag
    15181 tagactgata gtagtcccaa cctttccatc ctataaaagc aatcaatatg ataacgatca
    15241 accctaaaat ttttttcatg aaagatctcc ttttttattt ttat
//
LOCUS       DACDIG010000060        15217 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.060, whole genome shotgun sequence.
ACCESSION   DACDIG010000060 DACDIG010000000
VERSION     DACDIG010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.060"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(24..722)
                     /locus_tag="IV247_002071"
     CDS             complement(24..722)
                     /locus_tag="IV247_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAP8778132.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(715..2067)
                     /locus_tag="IV247_002072"
     CDS             complement(715..2067)
                     /locus_tag="IV247_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAP8778133.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2290..3690)
                     /locus_tag="IV247_002073"
     CDS             complement(2290..3690)
                     /locus_tag="IV247_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAP8778134.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3851..4588
                     /locus_tag="IV247_002074"
     CDS             3851..4588
                     /locus_tag="IV247_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAP8778135.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4713..5192
                     /locus_tag="IV247_002075"
     CDS             4713..5192
                     /locus_tag="IV247_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAP8778136.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5189..5956
                     /locus_tag="IV247_002076"
     CDS             5189..5956
                     /locus_tag="IV247_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778137.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6186..6755
                     /gene="lepB"
                     /locus_tag="IV247_002077"
     CDS             6186..6755
                     /gene="lepB"
                     /locus_tag="IV247_002077"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP8778138.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6859..7461)
                     /locus_tag="IV247_002078"
     CDS             complement(6859..7461)
                     /locus_tag="IV247_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP8778139.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7567..8036
                     /locus_tag="IV247_002079"
                     /pseudo
     CDS             7567..8036
                     /locus_tag="IV247_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8142..8726
                     /locus_tag="IV247_002080"
     CDS             8142..8726
                     /locus_tag="IV247_002080"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP8778140.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8729..9802
                     /gene="prfA"
                     /locus_tag="IV247_002081"
     CDS             8729..9802
                     /gene="prfA"
                     /locus_tag="IV247_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAP8778141.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9795..10634
                     /gene="prmC"
                     /locus_tag="IV247_002082"
     CDS             9795..10634
                     /gene="prmC"
                     /locus_tag="IV247_002082"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAP8778142.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10627..10806
                     /locus_tag="IV247_002083"
     CDS             10627..10806
                     /locus_tag="IV247_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778143.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10828..11853
                     /locus_tag="IV247_002084"
     CDS             10828..11853
                     /locus_tag="IV247_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAP8778144.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12028..13272
                     /locus_tag="IV247_002085"
     CDS             12028..13272
                     /locus_tag="IV247_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAP8778145.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13425..14372
                     /locus_tag="IV247_002086"
     CDS             13425..14372
                     /locus_tag="IV247_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8778146.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14506..15135
                     /gene="upp"
                     /locus_tag="IV247_002087"
     CDS             14506..15135
                     /gene="upp"
                     /locus_tag="IV247_002087"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAP8778147.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
BASE COUNT         5188 a         2720 c         3111 g         4198 t
ORIGIN      
        1 tcacattccc ctttttcgaa taactaggct gtttgtcctt caagataggg cagatccaat
       61 tctttaccgt aacgttgttc taaagctagg cggatcaaac gagctgccag gttctcattt
      121 cttgcaagga taggtccatg gaaataagaa ccgaagacat ttcggtaaat gacaccttca
      181 gattggtctt caccattatt tccttgacct tgtaccactt tccccaaagg tcgttcacct
      241 tcacctaaga aagtccgacc attgtggttt tcaaatccat aataagtttc attgaattct
      301 tcgttatgga taacgatatc accgataaac cggttatttt cctgacttaa cgtgtagtga
      361 tccagtgcac tgattccttg gatcttttct ccattagctc ccatgtagta gtgacctagc
      421 aattggtagc caccacaaat ggctaacatg acgccatcat tttcgatata gttgatcaaa
      481 gaatctttct ttttctggat gtcttttgaa atgattaatt gctcaaagtc ttgaccaccg
      541 ccaaagaaaa ccaagtcata tttatcagca tcaaaaggtt catgtatact aacgatctcc
      601 gtatggcaag aaacccccat tttttctgct atatatttca acatcaaaag gtttccgtta
      661 tcaccatatg tgttcatcaa atttccataa aggtgggcga tattgagttc ataattagcc
      721 acggttcatt cctcctttga taaatccttg agcagctagt tctttacgaa gctgcaatac
      781 agcagtgtaa gtagctaaaa tatagacatg ctcagttggc aattctttga ttttttcgat
      841 tacttctggt aattcttttg tttctgtcaa tttattttct ggaatcccgg caacttttag
      901 acgtaaagtc atatctgtat gacggtctcc tccagaaata acagctggga tatccatatc
      961 ggcaaagttt tcaaaattac catcccaaat ccagctgaca tcgattccgt ctgcgtaatt
     1021 ggcatttaat aaagccacta aagaaaatgg ataaggagct agaccaatca tatcgattac
     1081 ttgattgata cctacaggat ttttgacaag tactaatgta cattttttcc cattgatatt
     1141 gatgacttct tgtcggccga atactttctc gtcatatcct aaaccttgtt tgattttatc
     1201 aggatcgaca ttgaagtact ctgccactgc agtagctgct aaagcattgt agacattata
     1261 cataccgcca acttcgattc cgtattcatg accatcgatc acgaaatctg ctgacacatt
     1321 gtccatccgt accatatcag ttaatcgata atccaattca ggacgtttga agtcacaatt
     1381 tggacagtaa tatttcccta agtttgcata agtgatcatt ttataatgca agatgtgatg
     1441 acaatttgga cataagacac cgtctgtatt gtaatgagcc atttgttcac gatcttcttc
     1501 atggtcaaag ccataatatt ttctaggatt gactgtttcg atagaattga agattggtga
     1561 atcaccgtta ctgataatcg ttgcatttgg tgactttgcg gcaccgtcta caatcatttt
     1621 ataggtcgta tagatttctc cataccgatc catttgatca cggaaaatat ttgtaaataa
     1681 gaacagttct ggtttcatat attctgtgac tttgctcaaa cttgcttcat ctatttctag
     1741 gacagcaaac tttttctgtc ctttttttgg ttttgcctgc aagaaagtcg atacaattcc
     1801 ctgtaccata ttcgcaccag tagggttggt tagaacttca tcgaattgct ggcgcaaaat
     1861 attgactgtt aaagcggtag tcaatgtttt accgtttgtt ccggtgacaa cgacgacttg
     1921 gtagtctttt gccaaactat ctagtatatg tggatcgact ttcagcgcta gtttacctgg
     1981 taaacttgat ccgcctttaa aaaaagtttt taatgcccat tgcgacgttt ttccaactgc
     2041 aatcgcaaaa tgactgcgta ttcccattat gtaacctcca aaaacgattt gctgacgaat
     2101 aaattcaact aatcatacca tatgttgaaa aaaaggttaa attgcttttc ttattcttaa
     2161 gaaaaggtta cggctgaaag tgcatgaaat cggacaaaat cgcatgattt aacaaaaaaa
     2221 cagcttgaaa caaaatgatt gtttcaagct gctgtgattt tattattccc tcttacctat
     2281 attcccattt taaccgataa taattttctc gcttggatat tcatagccta gatctcgtgt
     2341 ttcttttggc acgaataaca tcaatgtata tagcattccg atccgcccaa taaacatcag
     2401 tgtagcaatc gtgattttgc ccacaacaga caagtctccg gtaatcccta aagataaacc
     2461 agttgttcca aacgcagaag ttacttcgat gatgatcgag atcagcgttt gttcttctgt
     2521 agcagataaa aaaacgatac aaaagaaaca cataccaaga gaaagcataa agacaacgac
     2581 tgattttcgc acatcatctt gatcgattct ccgtccaaaa atattgatgt tatcttcact
     2641 tttgaggaag gaatatagat acaaaccgat gattgctaca gtagttgttc gaatcccgcc
     2701 gcctacagaa ctaggactgc atccgataaa catcagtaaa gaaaaaatga tcagtgttgt
     2761 gatttgaaaa tcacccaaat cgtgaatctg cagaccagca ttacgagtcg taatcgaata
     2821 aaacatcgaa ttgatccatt tgacactcat attggaatct tggaataaat ggtccttttc
     2881 caatagataa atcaagacag ttcccgaaac aaacaagata acaaaagcta acacagctaa
     2941 tttggtaaac aaggaaaaac gaaacggaag cttcgcattg gaacgtttgt ataatagcca
     3001 ttcccggcac tccatcaata caggaaaacc aattcctccg acaaaaatca aaaacatgat
     3061 caggatcaag aaaaaatagt catgtgcaaa gggcttgatg gaatccccag tgacgtcaaa
     3121 cccagagttc gtgacagccg atatcgcctg ataaaaaccg taaaaaacgg catcacgcca
     3181 acgatcaaaa tagcctctgt aataaaaata aatagaaaag aatgtcccaa aaagtaattg
     3241 aaaccagatt aaaatagcaa aagtgatccg aatcagtcgg acgataccag acaaacgcgg
     3301 ttgattcata tccgtcatga tcagctgtcg ctgtttcagt gtgatccgtc gtttagaaaa
     3361 aatgacaaaa gcagtagaaa tcatcatgat ccccaaacca ccaacttgaa ataatatttc
     3421 cagcagtata ataccgttat cattgaacac agaattgata tcaaaagtcg atagtccagt
     3481 cacactcact gtactgattg ccatgaacag catgtccaca aaagaaacat ggctatctgg
     3541 ttcgcgaaaa atcggcatat aaaatagaac gagtgataag accgtcatca atatataata
     3601 aaaaacgatg atttggatcg atgagaaatg attggcagca aacttctgtc ctcgccgctg
     3661 cagccttcga aagaataaat caacatgcac agtactatcc tcctgacaat agtcgtttct
     3721 accagtatac aaggggaatt aggaatactc aaagaaatta tcaaacagat aacaatacag
     3781 ctttcgatta aaatagaaat ggcttataat aaggaaaatc ctagaaaaaa gaaggaggag
     3841 gaataacaaa ttgattaatg ctcaacaaat cgtagaaaaa atgaaaaatc aaaaaataaa
     3901 ttatgataca gttctgaaaa aaatgatcat gcaatgggag aaagaagaag taaagcctaa
     3961 gatattaata catacatgct gtgctccttg cagtacgcac acattggaat tcatgtctca
     4021 atatgcagag atcacgttat ttttttcaaa ttcgaatatt catcctaaaa gtgagtattt
     4081 gcgaagatta catgaacaaa aacgatttgt agaccaattt aatgagaaaa ctgaccatca
     4141 tgttgcgcta atcgttgatg aataccgtcc aaatgaattc accaaaatgg ttttaacaaa
     4201 ccatcttgaa aacgaaaaag aaggtggtgc tcgctgcagt gcatgcttca atatgcgatt
     4261 agatcttgtt gcgcaaaaag cgcaagaact aggatacgat tactttggaa gtgccctgac
     4321 catctcgcca aagaaaaatg cagctttgat caatcagatc ggaatggata tccaaaagat
     4381 ttacgggaca caatacttgc caagtgattt caagaaaaac aaaggctatg aacgctcttt
     4441 gcaaatgtgc aaagagtatg atatttatcg ccaatgttat tgtggttgcg tgttcgctgc
     4501 caagcagcaa ggatgcgatc tgaaagaaat caatcgggaa gcaaaagctt acttaaaaac
     4561 atcaaccgta gtttttgagg aaaattaaag aaatctttcg gaaaaacgta agaaaaaaat
     4621 aaagaattta tggtagaaat aaggtagact gtataatttg tccgtagtaa agtaaggaaa
     4681 caagaataaa tttcaatgaa ggaagagata agatgcgaaa agtagcacct attttgaagc
     4741 caatcgctgt attaacaatc tcgacgattg tcgcgactac tacaggtatt gtgaatagtt
     4801 ccagctaccg tgcaccaagt tcaagttcta gtactgtaga agaaacaaca ccaactactt
     4861 cagtctcgac atctgaacca acaacaacgg aaagtttgaa tgaaaccact acgacaagta
     4921 catgggacga tacaacaact agttcgtcgt atgatgagac atatacaact gacagctcaa
     4981 gcgaaacaag taatacggta ccagatggca ctccatcgga ttcaaccaat ccaagtacaa
     5041 gtccggatac aagtgctagc tccgaaacga taccaagcag tacgactgta gatgataaca
     5101 cgcaaaatga tgatgcagca cgtacagcac agcaaaacca agattcagga aatacccaaa
     5161 cgtataacca gcaagcaggt gattctcaat gacattttta ctagacctta tcaacggggt
     5221 gcataaattc cttggctatt tagacatcag tcctaaatat cttaaccgtg gatatacgat
     5281 tttaagtgtg attcccacgc tttatttatt gagaattgtt tatggacttt ggcaaaacca
     5341 aaactatctt caatttttct tatatggact tgcatttcta gtgcttgttt attttactgt
     5401 tttaaatgta ttttattact ttttagataa aaacacgaaa gccgatgtca ctcagctttt
     5461 tgtgaagtat ttacctgatg aagcgttcaa catccagaca gaagaatcga tagataatgg
     5521 aaattcgatc gataaagtga atacacaaga ggtcatggtt tcttatgacg aagattatca
     5581 attgaaatta gccgagaata tgcggtattt gattggaaat ggtgagatca aaacaaatga
     5641 actcggtcgc atggatggat ttctggtcga tagaaataca ttgtatcctt attactatgt
     5701 caagaaaaca ggagagaaag agtatgttct aaatattgga cgaagctact cggacatgga
     5761 aaaagtaggg accattcgac ttgactcaac agaagacaca ttggaacctg tcggattgtt
     5821 catcgtcggc ggtgattttg tcaaagacgg gttacgctat catgagcctt atcgtctgaa
     5881 attgattgca aaaaaggcac agtcgcagga agaaccagtg gtttatagtc gtagccaaag
     5941 aagaagacat tcataatgtg atgaaacgcc gtgtatcagt ggttgcgata aaaacgttta
     6001 ttcggaataa gggggatgtg acacgtcttt tgtcacgttc cttttttata taacgagaaa
     6061 gataagacga acacatcact tattctttcc tttcatttat aaacattgta gaataaagaa
     6121 agtacaacga agtattgtga aaaaacgtaa taaataacga ggaaaataca agtaggaggt
     6181 aagctgtgaa agaatttttg aaaaattggg ggattttgat ccttgtatta gcggctattt
     6241 tattagctag agtgtacgtg tttacaccag ttactgtaaa cgggcattcg atggacccta
     6301 cattaagtga tggacaacga ttgatttcat ctaagatctc aaactatgaa cgaatggata
     6361 ttattactac aaaggaaccg ggggatgaag aacggatgat tgttaaacgg atcatcggta
     6421 tgcctggaga tacagtcaag atggaaaatg accagttgac cattaatggc aaaaagtatg
     6481 atgaacctta tctggatgag ttcaaaaaag aattttcaga agataagtta caaggagaat
     6541 atgcttatag ctcaggattc caagcacaag cagaaagttc atccaccttt acgagtgact
     6601 ttgaatatac tgtgccaaaa ggaaagtatc ttgttctagg cgataaccgc ttgatttcaa
     6661 aagacagccg aatgtttggg ttagtcgaca aagatatgat ccaaggaaaa gttgttttcc
     6721 gttattggcc tctttcagaa attaaaatca tgtgatgaaa aaataaaaag aagctgtgac
     6781 atgttttttg tcacagcttc tttttccgaa aattccttct tatttcttgg agttgctcac
     6841 tttttttgca atctcatttt aagctttttg gctgatggat aagaaaagcg aagcgttttt
     6901 gttcataaat agcttttccc cacgatggaa ggtatgacta gtagcagaga caaagcccgt
     6961 tttttccaat cgattattca attcttcttg gctaaagcca ttatggactt tcggatgact
     7021 aatttgttca ttcttgtcaa agtctacgat gatcaattga ccgccaggag ctaaaatttc
     7081 ataaagtttt gtaagaatat tctccgtatc aggaatgtgt aaaagtacaa gtgatagaag
     7141 gatgagatta gcttggacag caggaaattc gacagaagca tcagcgtgga tcgttcggac
     7201 atttttcaga tctgctgctt ggatcttttc ttctgccatt ttcagcatag tttctgaagc
     7261 atcggcaatg atcagttctt tgaatctttc cgcaagcggt aaactaacaa gcccagttcc
     7321 gcctccgtaa tccagcagga ctttttggtg gctgtcgcgc ggtagataag tagttagttc
     7381 ttcgcgaata atcttggcta atgcttgacg gtcaggggaa tcataatggt tagcgatgga
     7441 atcaaaaata ttttttgtca tcaaatagct ccttacgtaa aactttgaaa ttttaactta
     7501 ttatagcacg tgaaaagaaa gaaagcatgc tatactcaat tcaaaaaggg agaatctaga
     7561 aagaatatgc cgatatcgtt tgtaaaagat agagaggaaa aaggaaagtg tgtccgagaa
     7621 atattactag acttgcctga gtggtttggg cttcctgaat caacggaaaa atatatcgag
     7681 gaatcaagta aactaccttt atggtgtgaa aaaagaaaag aagaatattt aggctttatc
     7741 actctttcac agacaagtga agatactgcc gaaatctatt ctattgtatg ggaataaaaa
     7801 aagactatca tcaccaagga ttaggaacaa aactttttaa agaagcagaa ggttacgcag
     7861 caaaacatta caaatatttg caagtgaaga ctgtagacga ggggcactac tctatatatg
     7921 atcaaactat ttgtttctac gaatcattag gattttcaag gttggaagtt tttccaaatt
     7981 tatgggatga atggaaccca tgtttagttc ttgtcaaaaa attggaacaa aagtgagcaa
     8041 tagatttaat actaatctgt gcttgatgaa aattctcact gtaaaaaatg ctgaaaatca
     8101 agtataatac cataggaaca aactggaagg tgaaaaagag catggcacaa ctatttttta
     8161 agtatggcgc aatgaatagc ggcaagacga tcgagatcct aaaagtcgca cataattatg
     8221 aagaacaaaa taagccagtt gtcttaatga caagcggtat tgatacaaga gatggtttag
     8281 gcgttgtttc aagccgaatc ggactgaaac gtaatgcgat gccgatattt gaagaaacag
     8341 acgtatttga cgtaattcag aaaatggaaa gcaaacccta ttgtgtgctg atcgatgaag
     8401 cgcaattctt aaaaaagaaa catgtcattg cttttacgag aattgtggac gaattggata
     8461 ttccggttat ggcatttggt ttgaaaaatg attttcgaaa cgagttattt gaaggatcaa
     8521 aatatttatt gctttatgca gacaaaatcg aagaaatgaa aacaatctgt tggttctgcc
     8581 acaaaaaagc aatcatgaat ttacattata ttgatggaaa ccctgtttat gaaggcgatc
     8641 aagtgcaaat tggcggaaat gaagcttatt accccgtttg tcgaaaacat tactttcacc
     8701 catcaatgat tacagaagga gaataagcat gtacgatcaa ttacaagcaa ttgaagaccg
     8761 ttatgaagag cttggagaac ttttaagtga cccagaagtg atttctgata caaagcgttt
     8821 tatgcagctt tcaaaagaag aagcaaacac gcgcgaaaca gtcgaagttt atcgtcgtta
     8881 taaagaagta gtacaaggaa tcaccgatac agaagaactg cttggtgaaa aactggatga
     8941 tgaaatggct gaactagcaa aagaagaatt atctgaatta aaaaaagaaa aagaagtctt
     9001 agaagaaaaa atcaagatct tgcttttacc aaaagatcca aatgacgata aaaatatcat
     9061 catggaaatc cgtggagcag caggcggaga cgaagcagca ttattcgctg gcgatctttt
     9121 caatatgtac caaaagtatg ctgaagcaca aggctggaaa acagaagtga tggaagctaa
     9181 cgttaccgga atcggtggat ataaagaagt gatcatgatg atcaccggag acaacgtata
     9241 ttcgaaatta aaatatgaaa gtggtgcgca ccgcgtccag cgtgttcctt caactgaatc
     9301 gcaagggcgt atccatactt ccaccgcaac agttgtcgtg atgccagaag ctgaagaagt
     9361 agagatcgat attgaagaca aagatatccg tacagatatc tatcatgctt caggtgctgg
     9421 cggacagcac gtcaataaaa cagcatctgc tgtacgtttg actcacttac caacagggat
     9481 cgttgttgct atgcaagatg aacgttctca aatcaagaac cgtgaaaaag caatgaaaat
     9541 cttgcgtgct cgtgtgtacg ataagatcca gcaagaagca caaagcgagt atgatgccaa
     9601 tcgaaaatca gcagtcggaa ctggggatcg ttcagaacgg atccggacgt ataatttccc
     9661 gcaaaatcgt gtgactgatc atcgtatcgg attaactatc caaaaattag atcagatttt
     9721 agcagggaaa ttagatgaga tcgttgatgc attgatcatg tatgatcaaa catcaaaact
     9781 ggaagctatg caaaatgggt aaaatgacct atcgagaagt cctgtcacgg gcttcttctt
     9841 ttttagagga tcatgggaaa gaagcatata gtatccaatt tttatttttg gaacgaaaac
     9901 aatggaaaaa acttgattgg ctccttcata tgaacgaaga gatttcagaa gaagaacagc
     9961 ggttgatcga aaccgatctg cgacttctgc ttgcagatca tccacctcag tatttgctag
    10021 gatatgcaga tttttatgat catcggttaa aggtaactga agcaacactg atccctcgac
    10081 cagaaacaga ggagttagtc gaatggtgtt tagacgagac gcctggtgta ccgttagaag
    10141 tgatagatat tgggacaggg actggagcaa ttgctattag tttgaaagca gcaagaaaaa
    10201 attggcatat atctgctgtg gatctttcag aagaagcatt agaagtagcg aaagaaaatg
    10261 ctcagaaaga aggaacgaaa atcagttttt atcatggaga tacattagga ccagtaatgg
    10321 atcagcaatt tgatgtgatt atttctaatc cgccgtacat cagtcggaat gaatgggaac
    10381 tgatggatga aagtgtccgg tcattcgaac ctaaaatggc cctttttgct gagaacgatg
    10441 gattagctat ttatgaaaaa atagcagttg aagcaccaag cgtactaaaa tcaaatggaa
    10501 agattttttt agaaatcgga tttcgccaag gtgaagcagt aaaaaaaata tttcagcaag
    10561 catttccaga taaaaaagtg gcgataaaaa aggatctttt tggcaatgaa cgaatgatcc
    10621 aagtttatgg ttgaagttgt ggatgaaaaa ttttttattt gtttttttat tataaaaaaa
    10681 cacttatttg acaaggatgt aaacgacttt ttggtttatc aacaagttta tccactattg
    10741 acaaatagat tatccacaaa cagtggaaaa cttgaggata aactcagaca aaataagaat
    10801 gtatgaaaaa ggggttgtgg ataaactgtg gaaacgaaac gatatgatga aacagaattg
    10861 caagaggcag ccgaagcact gaaagaagga gaacttgttg cttttcctac cgaaacagta
    10921 tacggattgg gcgccaacgc tcttttacca aacgctgtca agaaagtgtt ttctgtaaaa
    10981 gggagaccgc aagataatcc attaatcgtc catgtcgcct cttttgaaca agtaaaagag
    11041 tatgtggaca actttcatcc ggcagcagag aagattgtga agaacttctg gcctggacca
    11101 ttaactttga tatttaagat caaaaaagac actttgccct cagttgtgac gggaggatta
    11161 tccacagctg catttcgtat accggataac aaaaaaacac tagaagtaat cgaactttcc
    11221 ggtgttccgc ttgtcggtcc aagtgcaaat acgtcgggaa aaccaagtcc aacgactgca
    11281 gatcatgtat atcatgatct acaaggaaaa attaccggga ttattgatga tggagctacg
    11341 cgaattggtg tagaatccac tgttcttgat ttaagtgatc caacagccat gcctatgatt
    11401 ttacgaccag gtgcagtaac gaaagaacaa atcgaagcag tcatcgaatc tcctgttgct
    11461 attgatcagc atcttgtaaa agaaaatgaa acaccaaaag cccctgggat gaaatacaaa
    11521 cactactcac ccgatacacg tgttctgatg gtcagagatg gcgattggtc aactgcagtt
    11581 caatgggcaa aaaataagaa gatccgtgca ggtgtcatag caagccccga aattgcggat
    11641 caagtgcgga cagatactgc agctgtttat atgtataacg acaactcagt agaagcggcg
    11701 gctaaaggat tgtttgcagg tctaagagga ttagatgaac caacacttgg attagatttg
    11761 atttttgtac aagtgtatcc agaaactgga ttggggaatg cttacatgaa tcgcttgaaa
    11821 aaagcagcag gacaaaatta ttttgaaaaa tgatcaattg gttattgtcg cattctttta
    11881 gtatgataca atgctatcaa gtaaatcttc tatcagtgaa cgaaaaacta agcgtaacta
    11941 atcgtaatag aagagatacg aatatttggc aatttgtttt tgatgggaaa aataaaattg
    12001 taggaaaata aaaaggagct gttgtttgtg gtagattaca aaacgtttga ccctgatctt
    12061 tgggctgcga tcgccaaaga agaagaaaga caagaacata atttggagct gattgcctct
    12121 gaaaactttg tctccgaagc agtgatggct gcacaaggaa gtatcttgac aaataaatac
    12181 gcagaaggtt atccagggca tcgctattat ggtgggtgtg aatttgtcga tatcgttgaa
    12241 aatttagcga ttgaccgtgc aaaagaatta ttcggagcga aatttgcgaa cgtccagcct
    12301 cactccggct ctcaagccaa cacggctgct tatctcgctt tggtagaacc aggggacacg
    12361 atcttaggaa tggatctttc cgcaggcgga catctaacac atggatcacc agtcaacttc
    12421 agtggcaaaa cctatcactt tgttgcttac ggtgttgatc cgacaacaga agttattgat
    12481 tataatgtgg tacgtatttt agctagaaaa catcaaccaa aattgatcgt agcaggtgcc
    12541 agtgcttacg gacgaacgat cgactttgcg aaattcagag aaatcgcaga tgaagtaggc
    12601 gcaaaactga tggtagatat ggcacatatc gcaggattag tagcagcagg acttcatcct
    12661 aatccagttc cttatgcaga tatcacaacg actaccactc ataaaacatt gcgtggccct
    12721 cgaggcggca tgattttgac aaacgatgaa gcattagcaa aaaaaataaa tagtgcggta
    12781 tttcctggta ttcaaggcgg ccctctagag catgtgattg cgggtaaagc agtagcattc
    12841 aaagaagcat tagatcctgc atttaaagaa tatagtgaac aaatcatcgc caatgcaaaa
    12901 gcaatggtca aagttttcaa tcaagcaatt ggaaccagag tcatttcagg ggcgacagat
    12961 aatcatctga tgttgatcga tgttcgcgag ttaggtataa atggaaaaga agcagagagc
    13021 attttggaca gtgtgaatat cactgtaaat aaaaactcga ttccttttga aacactaagt
    13081 ccattcaaaa caagcggtat tcgaatcggc acgccagcaa tcactactcg cgggttcaaa
    13141 gaagaagatg ctgtaaaagt ggctgaacta gttgtcaaag cattacaagc aaaagatgat
    13201 aacgctcaac tagacgaagt caaaacaggt gtacgcgaat taactgaaaa atttccgttg
    13261 cataaaaaat aaaaatttaa attccattag taaacagaaa agttcaatgt tgggcttttc
    13321 tgtttatttt ttgggaaaac ggattctttg acaatatcgg tgatgctctc tagtatatga
    13381 ggtgtaattt tagtcttcac actaaatctc agggaggaaa agtcatgact gatcgtatct
    13441 cgttagaaaa ggaagcagta gcattcagtg atgcaaatgc gcctcatccg cgtatctatg
    13501 aactagcacc ggaagaagga agagttttac tagaagaagt acaagaatca cctgtgaaca
    13561 aatattccgt cgatattgaa gacttgatgg tggatactgg cgaatgggga aaactcaata
    13621 ttcgtctcgt acgaccagaa gggaatatgg ataaattacc agttattttt tatattcatg
    13681 gagcaggctg ggtttttggc agtgctcaaa cccatgataa attgatcaga gaacttgcgg
    13741 ttcgtacgaa ttccgtcgtt gtctttccag aatatagtcg atcaccagaa gcaaaatatc
    13801 caacagcaat tgaacaaaat tatgctgtgt tgcaacagct gaataaatta gccaagtcaa
    13861 aaaatctgga tctcgagaat ctaatcgttg caggagattc agtaggtggt aacatggcta
    13921 cagtcatgac gatcttaaca aaagaacgaa agggcctccc aatatccaag caattgctgt
    13981 attatccagt cacaaacgct gattttgata cagattccta tcatgaattc ggagaaaact
    14041 attttttgac aagagaagga atgatgtggt tttgggacca atatacgacg aatgaagaag
    14101 agcgtgctga aatcacagct tcgcctttga gagcaaatat aaatgaacta aatgatcttc
    14161 cttcagcaat gattttaacg ggagaagcgg atgtattaag agatgaaggt gaggcttatg
    14221 cgcgcaaact tcgtgatgca ggggttcctg ttacacaagt acgcttccaa ggaatcatcc
    14281 atgattttgt catggtaaat gctatggatc agacaaaggc aacgcgtgca gcaatggcat
    14341 tatctacttc ttggatcaag aaaaaagaat aagctttgct tgtccgcagg aaaacttctt
    14401 ttcaaaaacg gattttctta ggaaaaatgg cgagtttgac tagtcaatca cttgattttg
    14461 ttttacaatt aaaaagaaac gacagaacaa aggagagctt aaaaaatggg caaatttcaa
    14521 gtgatcgatc atccattgat tcaacacaaa ctaactatta ttcgggacaa aaactgcgga
    14581 acaaaggttt ttcgtgaagt agtcgatgaa atcgctatgt tgatggcata tgaagtatca
    14641 cgggatatgc cattggaaga catcgtgatc gaaacaccga tcactgaaac aacacaaaaa
    14701 acgttgtctg gtaaaaaagt agcgattatt ccaatcttgc gcgcaggtat tggaatggtt
    14761 gacggtatct tggaattgat tccagctgct aaagtgggac atatcggtct ttatcgagat
    14821 gaagaaacat tggaaccaca tgaatatttc gtcaaacttc cagaagacat tgatgcacgt
    14881 cagttgtttg tagtagatcc aatgttggca acaggcggat cagcaatcat ggcaatcgat
    14941 gcattgaaag aacgtggcgc gtcgaacatt aaatttgttt gtttggtagc agcacctgag
    15001 ggagtaaaag ctttgcaaga agcgcatcct gatattgaca tctatacagc ttctctagat
    15061 gaaaaattga atgaacatgg ttatatcgtt cctggactag gggatgcggg tgaccgtttg
    15121 tttggtacaa aataagtaaa tacgaaaaag ggtgtgacac aacttttgtc acatcctttt
    15181 ttggtcattt ttaacgtttg atgcagaaca gcttttt
//
LOCUS       DACDIG010000061        14966 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.061, whole genome shotgun sequence.
ACCESSION   DACDIG010000061 DACDIG010000000
VERSION     DACDIG010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14966
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.061"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(21..251)
                     /locus_tag="IV247_002088"
     CDS             complement(21..251)
                     /locus_tag="IV247_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778148.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            complement(687..1253)
                     /locus_tag="IV247_002089"
     CDS             complement(687..1253)
                     /locus_tag="IV247_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP8778149.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1253..2209)
                     /locus_tag="IV247_002090"
     CDS             complement(1253..2209)
                     /locus_tag="IV247_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAP8778150.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2209..2865)
                     /locus_tag="IV247_002091"
     CDS             complement(2209..2865)
                     /locus_tag="IV247_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP8778151.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3235..5253
                     /locus_tag="IV247_002092"
     CDS             3235..5253
                     /locus_tag="IV247_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8778152.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            5680..8094
                     /locus_tag="IV247_002093"
     CDS             5680..8094
                     /locus_tag="IV247_002093"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAP8778153.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8442..9995
                     /locus_tag="IV247_002094"
     CDS             8442..9995
                     /locus_tag="IV247_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP8778154.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10376..12160)
                     /locus_tag="IV247_002095"
     CDS             complement(10376..12160)
                     /locus_tag="IV247_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8778155.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12175..13896)
                     /locus_tag="IV247_002096"
     CDS             complement(12175..13896)
                     /locus_tag="IV247_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8778156.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13996..14757
                     /locus_tag="IV247_002097"
     CDS             13996..14757
                     /locus_tag="IV247_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8778157.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4853 a         2762 c         2950 g         4401 t
ORIGIN      
        1 ctcattaaaa agcataacca ttactccgat ctgtttttaa aattagtttc tacctctaat
       61 atatatcgag tttttatttt gccttcagag aatactgttt ctttttttgc agttacaggt
      121 tgtttatttt cggaaaaagc taatatagct aagattgaaa catccatctg gaatttatct
      181 tttttactgc tttgatcata aaagtctgca tattcatcac tgatattttt tctaataaat
      241 tcttccatca taaaaatcac ctcaaaacga ttatacgaac ttacgttctt gttgtaaagc
      301 gatatttgag gggcaaaaaa ggggcataat ttgactgata ttaagttagc tattgatata
      361 tgaatgacat acaaaaatat aaaatctttt aaaaatgctt atatatcaat gtttttgcca
      421 ttcttttata tatctgttat atgtcaaaaa aatgtgttca atttcaattt gtatttgaat
      481 aaagaggttg tgaaaaagct gtcctgcatc aagtaataag aacagccaat caaaaacagc
      541 ttctcatgtt tttgatttgg ttggacttat taccgcagat ataagctttt gagcaccgtg
      601 aataaagagg ttatgaaatc aacgctctaa cctgagtatt agagaaaaaa acgcaaaaca
      661 tatcatttat gttttgcgtt ttttatttat ctttttttct caatacaata taaaataggc
      721 ggctggttct tttggttgat aaaccgataa ttcagcacat tatatgtttc ctgtggcagt
      781 ttggaacaaa aagaatcaac catatccagt tctttttctc caccttcatg accatagtag
      841 acgacaagga tcaatcgtcc gcgaggcaca agtcgtttca atatttcttc catagctgtg
      901 cgtgtagtct ctggcatagt gatgattgct ttatcacttt ttgggagata ccctagattg
      961 aaaatccctg ctttgagatt ttgttgttca gctatcactt ctcctaacgt ttcgtggcct
     1021 tgtagaaaca atgagacacg ttcttctaac tgacgctgtt ctagcctttc acgagtagca
     1081 tcgattgctt gttgctggat atcaaaacta tagacatgac cagtttttcc gatgtgttct
     1141 gctagaaaaa ccgtatcgtg gccattgccc atcgttgcat ctacgacgtg atcccctggt
     1201 tgcaagattt cttgcagtaa tgtatggctg aaatgcaaag cagtttgcaa catcagacgc
     1261 tcaccttttt ccgtacatca tattttcctt ggaagctatc tctgcgaaga agttcttcat
     1321 caattgcatt caagacttcc cattttttca agctccacat tggtccaatc aaggaatccc
     1381 acggcgcatc ccctgtcaaa cgatggatga tgatctcttt tggtatcatt tccagctggt
     1441 cgcaaatgac attgacatag tcaggacggc tcattaattg aaggcgacct tcatgatagt
     1501 cccgcaacat tctcgttttg cgcatcagat gcatcaaatg gagcttgatt ccttggatat
     1561 ctgaatctaa aatggtgcgt ctgacatttt ctttcatcat ttctaaactt tcgcctggta
     1621 atccgttgat caaatgcgta caaacacgga tattatgttt gcgtaattta gccacgccat
     1681 ctaaataagt ttgataatca tgtgctcggt tgatcagttt tgaagtttct tcaaaagttg
     1741 tttgcaatcc aagttctacc cataaataca tccgctcgtt cagttcagca aggtaatcaa
     1801 ctacttcatc tggcagacaa tccggtcgtg taccgactga taatcctacg acacctggta
     1861 aattgactac ttgctcaaat cgttctttga ttatttcaac tggcgcatgg gtgtttgtaa
     1921 aattttggaa ataaacgatg tattgttgga catgaggcca tttttgatgc atcatgtcga
     1981 tttctttctg gaattggatt ggaagagggt cttcaggtgc aacgatcatg tctcccgaac
     2041 cagatacact gcaaaaagta caaccgccat gggcaacagt cccatctcga ttcggacagt
     2101 caaatcctcc atcgatcggc accttgaaaa ttttcccgcc aaactcattt cgtaaagcgt
     2161 aattccaaga atgatatctt ttatttcctt ctgaataagg aaaagtcatt aaaagtcctt
     2221 ctttctgtaa gagttatcta tttgttttct tttttctcta tacactggat aagtcaggca
     2281 cagccaagaa agactgagta agtagccgcc gatgatatca gtaggaaaat gaacgcccag
     2341 ataaatgcgg cttatcccga cacttactat aataaacatc atcagcaatt gaagcaatgt
     2401 gcgtaaggtt cttttttgaa caaaagctgg aagcagaaat agtaaagaac cgtaaagaat
     2461 catactgcct gctgcatgtc cactagggaa actgtaactt gtttcaacta ctaagtgcgc
     2521 caatgaagga cgctctcgca tgaaaaatag tttgatcacc gggttgatga ttccagcaat
     2581 tacaataaat ccagaaacga accaaaatgt ttctgccctt tttttcttat accacatcca
     2641 taaagccgca caagcaaata aaatgatgac ggtaagagga ttaccgaatt tagtcataaa
     2701 gagttggtag ctgttcagat caggataaga agctctgaca atgcctgtaa accactgatc
     2761 gaaaccagaa agccacgaca gatggaactt tacgatccag cccaaaatga aaaacaggac
     2821 aagaaagaag ctaccagcca cctgaaatga tgttttatat ttcatacaat tcctcctttg
     2881 aagcaatgac ttattatagc atttttggtc cagcgaaaaa aacggtaaag agataaaacc
     2941 aatgtatgat tcctctttga ataaccaata ttcttataaa aaaaataact gtaatcggaa
     3001 agacattttt tggtaatgca aatgaaatgt tagggaaata aaatcagtgt ttttcgaaat
     3061 ccttttaaac aagaacgtag acgtaggaag aacttgaaaa tgaacagaag ttgttcatta
     3121 cggtaaatta caatatgatt acaaaaaaca acaaagctct taaataactt ttaataaata
     3181 ttaagagaag ttataattat taataataga taaattgaag ttggaggata gaagatggaa
     3241 aacattgcaa gaaaagaacg gagacgttta gaacagacca aacgttttcg aaaagcaaaa
     3301 cgaggggcag caatcgtggg aactgcgatg gtaggctgtt ctgtcgctgc cccattagta
     3361 cagccggttc ctgttcaagc ggatgaaaca cctgttcagt tcggcgcaag aatcaataca
     3421 gcggcattta tcacagaaat tgctacttat gctcaaccaa tcgctcaagc caacgatcta
     3481 tatgcatcag tgatgatcgc acaagcagtt gtagaaagcg gttggggcag tagtgcgtta
     3541 tctcaagcac cttattataa tctttttggg ataaaaggca gctatcaagg acaaaccgtc
     3601 tacatggata cgcttgaatt tttaaataat caatgggtaa ataaaaagga gcctttccga
     3661 caatatcctt cttttgctga atctttcaat gacaatgcct atgtacttag aaatacttca
     3721 tttggcaacg gttattacta tgccggtact tggaagagca acacgaaatc ctatacagat
     3781 gcgacagctt gtttgacagg gcgttatgcg actgatcctg gttacgcagg caaattaaat
     3841 aatatcatcg caacttacgg attgacccaa tatgatacac cagctagcgg aaacgcaggc
     3901 ggtggagcga ctacaggtgg caataatact ggaagtgcag gtagtactgg cggttcaagc
     3961 aattctggta atacaaactc aggttcagca acttcgacca cttatactgt ccaatctggt
     4021 gattctgttt ggggaatctc aaataaattc ggtatcacaa tggatcaatt gatccaatgg
     4081 aacaacatcc aaaacaattt catttatcca ggacaaaaac tgacaatcaa aggtagccag
     4141 gaaaatggct cttcgacgaa taattccggt aacaatacca actcttcagg aaatgctggt
     4201 acatcaaacg gaggacaaac aactggtgcg aaatacactg ttcaatctgg tgattctgtt
     4261 tggaagatct caaatgatca cggaattacg atggctcaat tgattgaatg gaacaatatc
     4321 aagaacaatt tcgtttatcc aggacaacag ctaatcgtat caaatggcgg ttcttctaat
     4381 gctggagcaa atactggctc aaatactggg aatacaagtg gctctagttc gtccagctca
     4441 agttcttcta atacagcttc aggaacgaaa tatacagtta aagccggaga ctctgtttgg
     4501 agcgtctcaa ataaatacgg aattacgatg gctcagctaa tccaatggaa caatatccaa
     4561 aataacttta tctacccagg acaacaactg actgtttcaa acagtggatc aacaaacagc
     4621 acgtcaggta atacaggttc gtcaaccaat acaaataaca gcagttcagt taatactggc
     4681 agcagttcgt ctcaaggcac ttataccgtg aaagcaggtg aatctgtttg gagcgtggca
     4741 aataaaaacg gcatcacgat ggatcagtta atcgaatgga ataatatcaa aaacaacttc
     4801 atttatccag gacagcaatt gatcgttaaa ggtggaactt cggcaaatac aaatacaggt
     4861 tcaactactt ctgcagcgaa accaaataca ccgaatacgt ctgctacgac aagtacatca
     4921 tccactggta atacgatgta taccgtgaaa gcgggcgagt cagtttggag cgtagcgaac
     4981 aaacatcata tcacgatgga tcagttaatc gaatggaata atatcaaaaa taatttcatc
     5041 tatccaggac agcaagtgat tgtgaagaaa ggttcatctc aatcaacaaa ccaacaatca
     5101 gttactggtt cgaaaaccta tactgtgaaa tctggagaat ctgtttggag tgttgctgac
     5161 agtcatggaa ttacaatggc tcaactaatc gaatggaaca atattaagaa caactttatt
     5221 tatccaggtc aaacgttgat tgtagctaaa taaaatagct gttcgaagaa ataacgattg
     5281 aattccacaa aaatatttgt tactataagg ctacttaaat atgacaacac ttgaaaagga
     5341 agtagtaaaa agactgaagt ttcagagagc gcatggctgg tgagaatgtg cacggatgct
     5401 tttgaactcg cctttgagtt gcccttgctg aatacccgag taagcaagcg gcggtgacgg
     5461 ccgttacaac gttcgaggcc ttttcactaa ggtaaaatta ggtggtaccg cgtagattac
     5521 gtcctataaa aagagcaatc tttttatagg atttttttat ttcttttgtg aagtttgaga
     5581 aaacaaagca aaaggaaata aaaataaaca gattctgccg gttttttcgt tatatttata
     5641 atagaaacaa gtgttttcaa tagaacagga ggacagcaaa tgagttacaa tcataaagaa
     5701 attgagaaaa aatggcaaaa atactgggca aaaacgaata ctttcaacac ccatgatgat
     5761 cctgaaaagc caaaatttta cgcactggat atgtttccct atccatccgg tcaagggcta
     5821 catgtagggc atcctgaagg ttatacggca acggatatcc tttcacgtgt gaaaagaagt
     5881 caaggataca acgtgctgca tccaatgggg tgggatgcat tcggtctgcc tgctgaacaa
     5941 tatgcgctag atacaggaaa cgatccagct gagtttaccc aaaaaaatat cgaaacattc
     6001 cgacgccaaa tcaattcttt aggatttagt tatgactgga atcgcgaagt gaatacaact
     6061 gatccggagt attataaatg gacacaatgg atctttacga agctttatga aaaaggtcta
     6121 gcttatgaag cagaagtagc ggtaaactgg gtacctgaat taggaacagt tatttcaaat
     6181 gaagaagtga tcgatgggaa aagtgaacgt ggtggctatg atgtagtcag aaaaccaatg
     6241 cgccaatgga tgctgaaaat cactgcttat gctgatcgct tattggatga tctagaatta
     6301 gttgattggc cagaaagcat taaagaaatg caaagaaatt ggatcggtcg ttcagtagga
     6361 gcaaatattg aatttaaagt ggctggtacg gataaatcct ataccgtatt tacgacacga
     6421 ccagatactc tattcggtgc aacttactcg gttcttgcac ctgaactaga tcttgttaga
     6481 gaaattacga cccctgaaca aaaagctgct gtagaagctt atatcgaaga gacagctaaa
     6541 aaatcagatc taaagagaac ggatcttgca aaagaaaaaa caggagtatt tactggtgct
     6601 tatgcaatca atccagttaa tggcaaggaa atcccaatct ggatcgccga ttatgtatta
     6661 tcttcatacg gcacaggagc catcatggct gttccagcac atgatgaacg tgactttgaa
     6721 ttcgctcaaa ctttcggact agaaattctt ccagttatcg aaggtggaga tgttgaaaaa
     6781 gctgcttaca cagaagatgg tacgcacatc aactctgaat ttctagacgg catgaacaaa
     6841 caagaagcaa tcgataaaat gaatgaatgg ctagaagaaa atggcgtagg gaaaaaagaa
     6901 gtcagctatc gcttacgtga ctggctattc tcccgtcaac gttactgggg tgaaccaatc
     6961 ccaatcatcc attgggagga cggaacagtc acagctgtcc cagaagaaga gctaccatta
     7021 cggttgccta agacaaaaaa catcaagcca agcggtactg gagaatctcc attagcaaat
     7081 atcgaggagt gggtcaatgt cgttgatcct gtaacaggta aaaaaggccg tcgcgaaacg
     7141 aatacaatgc cacaatgggc aggaagctca tggtattacc tgcgttatat cgaccctcat
     7201 aacaaaaaag aactagcaga ttatgaaaag ttgaagcgtt ggctaccagt tgacatctac
     7261 attggtggag cagaacatgc ggtacttcat ttactatatg cacgattctg gcataaattc
     7321 ttatacgata tcggtgttgt tcctacaaaa gaaccattcc aaaaactata caatcaagga
     7381 atgatcttag gcgaaaacaa cgaaaaaatg tcaaaatctc gtggtaatgt agtcaatcct
     7441 gatgatgtgg tcgaaactta cggtgcagat acactgcgca tgtacgaaat gttcatgggg
     7501 ccattagatg cttcgattgc ttggagcgaa aatggcttag aaggaagccg taaattcttg
     7561 gatcgtgtat ggcgtttgat cgttgatgag aacaataaaa tgcgtgaccg tatcactaca
     7621 ttgaatgatg gcaagcttga taaagtgtat catcaaacag tgaaaaaagt gacagaagac
     7681 tatgaaaatc ttcatttcaa tacagcaatc tcccaattga tggtctttat caatgaagcc
     7741 tataaagtgg acgctctgcc ttatgaatat atcgaaggat ttgttcaact actcgcaccg
     7801 attgctcctc acatgggtga agaactatgg gcaatccttg gaaatgatgg tggtatttct
     7861 tacgcaccat ggccaacata cgatgaagca gcacttgttg aagacgaagt agaagttgtt
     7921 ttccaagtaa acggtaaagt tcgtgcgaaa agcaatgttc cccgagactt aggaaaagat
     7981 gagctagaaa aagcagcatt agccaatgaa atagtgcaag aatatattga aggcaaaaca
     8041 gtcagaaaag ttattgcggt accaaacaag ttagtcaata tcgttgctaa ttaattggct
     8101 ctttgtcaat aaggactgat gagttgtgca aaatcaaatc tgggccagaa tgaacttcat
     8161 tctgtctcag attttttgtt atttcaaatt gattaattga ttgatcaaaa aggttttgat
     8221 tttcatgttc tcaaacacgt tcaatgtctt tttcttctga atggtcttgt aagagatctc
     8281 tgtaatcatc aaatttttgt ctattaatct tagtattttt tgatgtataa actacctgaa
     8341 attgagagta cataccaaat tcataaattt tttattagtg ggactagtga ttgtatggat
     8401 tttgacgcat aatgaatgaa gtatgagaaa aggagagaca aatggatcaa gaactattta
     8461 ttatcgaagt attatcagct aaacaaaaaa ttatcgagaa agcgaaaaaa agagcgaaaa
     8521 gaataatgtt caagctttta agcttagtat ttttctctgg tttgcttttt ttcggagctg
     8581 tatcgttcct atatcgccat atgagtgcag tagtccgttc agctgaaggg atgcaaaaat
     8641 taaataattt gtatcacttt tcgtggggag ttggcggaat aacaattgtt gtgcttattg
     8701 tttttcttct acttatttgg ctccgttacc atcaattgtc gcgtcatttc gatcaatggc
     8761 ttagtcaaag agcacaacag tttttacata ataaaccaac ttctcaagat gatgactatc
     8821 tttattttga gaagacttcg acgagtcaag cttttcggat acaaaaaagt gattgccagt
     8881 tattgctgag atcacttgat gatttaccta tctacttagg cgaaagcaaa tcaaaattcg
     8941 gaatgaacac attccatatt tttttactag aaaacgaacc cagatgggaa aaaagccagt
     9001 cttcgcctgg tggatacctg acaactaaga tgagtacatt agtcactggt tttttcctca
     9061 ttcttttagc tggaacaatc tattatggga gatacactca gccaaaaatg tcttctcata
     9121 taaatgctgc ttctcacatg acaatgaact caactaacga tacgcatata caaaagacaa
     9181 atgaagggtt gttagctgat cctaagcaaa aggtaaattt gacaacacaa aagataaatg
     9241 atctcaaact tgatacagag acgcactctc tctatatgac aacagatagt ggtgctaatt
     9301 gggtatttgt gccgattgat tcgcaatggt tgcgttttgg cgaatacacc ctgacaacgg
     9361 ggctgattcc tgaaggttat tggatggaca acacatatga tatttccgcc gatttctcat
     9421 ggtttatcta ttcttctggg gatgatgtac gtctgctgac ttcaagagat aatggtaaaa
     9481 catggcaaac gtctattatc acccaatcag ttcagccgat acgttatcga aaagcggtat
     9541 tttatggcaa cgatggtcta tttatctgta catcagccag tgggatgtct gcagaaactt
     9601 tgtacattta cactactatg gatggcggaa tcacttggat aaaaagtgga agtacaatga
     9661 tcaatcagcc agtacaaaat gtcagcttcg ttacaacttc tttaggtttt gtttcgacac
     9721 gtgaaagttt gttttataca aacaacggag gacgctcatt caaagaatca gttctgtcga
     9781 ttccggaaaa ctatcagaca ggggggatag atctttttca atcaccaaat gaagttactc
     9841 aaataagcgc gaatacgtta gagacaaaat tttatttgtt gaaaaaagga ccaatcgatg
     9901 cagggaaaat gtttgcctgc ctcttccgtt caactgataa cggggaaaca tggcaattcg
     9961 ttcagcaact atcgcaagta gaacaaggag attaaagaag agagattgtg agcctgacgt
    10021 tcagcaccga gtcacaagaa acaaattcaa aaaacagctt ctcatatttg ttgaatagtg
    10081 taactcgtga ttcggagttg acaagagcaa caagaagcga ttttgaagta tagcttctcg
    10141 tattttacaa gatcgtgtct tattgccgat aggttgtctc ttgaacaccg tttattcgta
    10201 aaaagggatt gtgacaagtc ttttgtcact acttaccgaa cgaaactgaa ggagctatcc
    10261 aaaaagtatc attagtatta ttctttgtgt ttctagtggc accactagta catttaaaaa
    10321 agctatcgat catggactaa tcatacatga tcgatagctt tttagataat gtttgttacg
    10381 ctatctgaaa ttgactatta taaagactgg cataaagcgt tggtgattgg agtagttcag
    10441 aatgtgttcc tttttcgacg atatcgccat tcttcattac aaggatcatg tctgcatttt
    10501 caatcgttga caaacggtgt gcaatcacaa aacttgtcct atcttttgtc acagcttcca
    10561 ttgcttgctg aatcaatgct tctgttcgtg tatctacgct agaagttgct tcatctaaaa
    10621 taacgacagg aggattcgct agaatgactc gagcaattgt caataattgt tgctgtcctt
    10681 gagataacgt gccgttttcg ctagaaatga tcgtatcata tccgtctggc agtgttcgaa
    10741 taaagtgatc acattgtgcg atttcagccg cttcgataat ttgttctctt gtggcatcca
    10801 ttttcccgta ggcgatatta tcagctaccg ttccttcaaa cagccaggta ttttgtaaaa
    10861 ccatcccaaa agaacgacgt agttctccac gtgaaagctt ggtaatatca agaccgtcga
    10921 attcgatctt tcctttattc agttcataaa aacgcatcag cagattgact aaagtcgttt
    10981 ttcctgcacc agttggacca acgatcgcta ccgtttgttt tggctttact gaaaaatcga
    11041 cttgattcat caatatttta tctgatgtgt aaccaaactg tacatttttg aaagctattt
    11101 ttcctttcgg ttgttctaaa agtcttgcat ctggaaggtc tggaatttca tctgtttcat
    11161 ctaatatctc aaatatccta tcgattgctg ccattgcgga ttggatcgag ttgatcacat
    11221 aagaagcggt agaaattggt tcagaaatct gattgatgta ctgaagataa gcttgtaaga
    11281 agccgatcgt cacaccacct gatagaacaa gcatagcacc gaataccgca cttatgatga
    11341 aggcgagttg attaatgaaa cgaatggccg gatagatagc acagttcata aattgagcac
    11401 gtttaaaagc gttgtattgc tttttattta cttcgtgtat agcagcttct gcatgagctt
    11461 gctgattgaa tgttttgacc actagattac cagctaagat ttcttccatt cgattattta
    11521 aaacacccag ttcatactga ttctgttcag aaatctgttt gtttttgttc gcaatcttag
    11581 ttgtaacaat tgaactgcca acgatcagca ataagacgag caaagtaagc ttgctttcga
    11641 tataaaacag catcagacca gcagcagcta ttgttacgac agaagtaaaa aattgattga
    11701 tacctgtcaa taacacttga gaaagttgat tgagaccggt agttgtccga ctaatgatgt
    11761 cacctacttg atgattgtca aaaaacgaca tcggtaatgt tttgaatttt tttgtgattt
    11821 cttttcgcac cctcaatgtg atcttttcgc ttaacgctgc cattgtccgt tcctgaataa
    11881 atgaagtgat gctgcttaag actgaacagc cgatcagcaa taagacaggg aacaacaatg
    11941 cttgtttgat atcactgaaa gaaagctgtc caatacctgt ttttcttatt tctgataaga
    12001 gatcatcgat cccgattccc atgatcaagg gcatggctac gactaatagg ttcccgaata
    12061 aactgcatag aaacaatccg taaaaatatt tatgttcagg tttgatcatt ttccaaaaac
    12121 gtatcaaagc acgcgtgttc aacgtttttt tcttttccat cgtatccctt ctttctatat
    12181 tccttgtgat tttgcaaact cctgataagt ttggcaatta gacagtagtt cttcatgagt
    12241 accttgtcca acgatttttc cttcatctaa aacaatgatg ttatctgctt gttggattgt
    12301 actgaggcgt tgagcaacaa tcacgagagt tttctccgac atgtgtttat gaagggctgc
    12361 tcttaatgct gcatctgttt tatagtctaa agcggagaaa ctatcatcga atacatagat
    12421 ctctgctggt ttgatcaacg ctcgagcaat acatatccgc tgtttctggc cccctgaata
    12481 attagaaccg ccttgagcaa caaagctgtc caatccgtca ggaagggtgt ccactagttc
    12541 ttctaattga gcgatcttga tcgctttaga caactcttct gttgaggcat attcatttcc
    12601 catctgcaag ttgcttaaaa ttgtcccgct gaataagaaa gccttctgag ggacataact
    12661 gatgtgggag cggatctgtt cttgagtcat ttgagtaaga ggaataccac caagggttac
    12721 ttttcctatc gtagggtcac taagtctcaa gagaagtttc gccaaactac tttttccaga
    12781 tccggtcccg ccaacgatcg ctgttgtttt tcctttaggt attgtgaaat caatatcttc
    12841 tagaactgga tcagagattt cagtatattt aaatgtgaca tgttcaaaag cagctgcatt
    12901 tgtctgatca aaggttggtt cagctactgt tttgatcgtc tcttcctcaa tttcgatctc
    12961 tgcttgcagt atttcttcta tccgtcgtaa tgaggcaaac gatttaggga taatgaccaa
    13021 taccatagct gcgatcatca agtaagcaag tgtcagcata gagtattcga ctacagcagt
    13081 cacgacaccg atttgtaatg ctccttctgc tacaagattt ccgccaaacc acaaaataga
    13141 agcataaacg attcccatga gcaaaaaagc aattggagtg acccaagcaa atattcggtt
    13201 cactttgatc atttgattag catagtcagc aaagctctta ttcgttcgct tttcttcata
    13261 tgattgatta tcaaaggcac ggatcatatt aatcccagta aagaattctc tcaatgtgat
    13321 ggtgatccga tccatttttg gctggatact caaagataaa ggtgcccctc gtttcatcag
    13381 tcgataaaca atccatacat aaaaaacaac agagatcaat gtaacccatg ttagtaatag
    13441 agaataacga aacatcatga gaatggtgaa aacgcaaata accggtgctg gtaagatcat
    13501 ttggcaaaat aaaacaatca tttgctggac attatcgaca tcattcgtca ttcttgtcaa
    13561 aagagatcca gaactaaaac gactgatatc cttcattgac agctgttgga atttacggaa
    13621 atatttctcc cgcgtttgga atccaaacct agcggaagcc aatgctgaaa agtaactaga
    13681 ggcaactgca gcgattgttc ctagaaacgc tacaagcagc atttcaactc caattttttt
    13741 tataactgag gcttcgccac ttgctatacc tgtgtcgatc agctttgcta ctaattttgg
    13801 tacacctaat gtaccggcga tttctaaaca gatcatcaca aaggtaagga gagcgagtcc
    13861 tttgtttttc tgtaagaatg tttttaagat agacatatta ttcctccttt tttcctgata
    13921 cttttcgatt ctataccttc aagtaacttg aaggtcaata gaaaaaagaa taaactgaaa
    13981 aagaggtgag aaaaaatgaa aaaaatgtat acgattggcc aagtatcgga actatttgat
    14041 ttgccaaaat caacgatccg ctattgggat gaacaaggat taatccgttc aagtagacaa
    14101 gaagagaatg attatcgatt gtttgatatc gatgatattt ttatgcttta tgatatcgtt
    14161 ttttatagaa aattagatat accgatcaaa cagatgaaga atttatatgg aaaaacattg
    14221 acggagttat atgagacact tgatgaaaca gaaagaagaa tccataaaga actggtggtg
    14281 atgaaacaaa aacaaaaaga gataagggag caaaaaaaac agttgaaatt gatgatcgat
    14341 acaaacgagg aagaatttcc tgtagaagag atcccttttg actgtatgat ctcaacggag
    14401 tttgaagata ttgtggaaat caaaaaattt ctacctaact attcaagctt tgggatgatg
    14461 agtatgccag cttccagttc acagacgatg tatggatttt ttatcgaccc tagtgaagta
    14521 catttattta ctcaagacgt gatttgggag aaaaaagata cggctgttta tcgtcgattc
    14581 ttattgaaat ccgagatgaa ccatgcggaa cgtaataata ttttagaaat cagagaaaga
    14641 atgtatgaaa aaggatggaa aacaggagaa gtgatcggcc agtatctatt gacgaatacg
    14701 gatgaaaaca atatcagaac tgaatattat catgcatgga tagaaatgaa aaaataggaa
    14761 atatgtgttt aagataactc gaaaatgatg gttgttttag ttataaagaa aaattttctg
    14821 gttcgattac gggtttaatg tgggggatta ttagtcgtgg atagatagaa ttttagtttt
    14881 ttagaggaaa tatgtcttta aaaatggaat ggttgcaatt cttagcgata tatacgatgt
    14941 aattgtcctc ttaaaaaagt atggaa
//
LOCUS       DACDIG010000062        14737 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.062, whole genome shotgun sequence.
ACCESSION   DACDIG010000062 DACDIG010000000
VERSION     DACDIG010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.062"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(547..909)
                     /locus_tag="IV247_002098"
     CDS             complement(547..909)
                     /locus_tag="IV247_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778158.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(983..1363)
                     /locus_tag="IV247_002099"
     CDS             complement(983..1363)
                     /locus_tag="IV247_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAP8778159.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1445..2620
                     /locus_tag="IV247_002100"
     CDS             1445..2620
                     /locus_tag="IV247_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP8778160.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2696..3280)
                     /locus_tag="IV247_002101"
     CDS             complement(2696..3280)
                     /locus_tag="IV247_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8778161.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3481..4479
                     /locus_tag="IV247_002102"
     CDS             3481..4479
                     /locus_tag="IV247_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8778162.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4591..5100
                     /locus_tag="IV247_002103"
     CDS             4591..5100
                     /locus_tag="IV247_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAP8778163.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(5130..5789)
                     /locus_tag="IV247_002104"
     CDS             complement(5130..5789)
                     /locus_tag="IV247_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAP8778164.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5791..6555)
                     /locus_tag="IV247_002105"
     CDS             complement(5791..6555)
                     /locus_tag="IV247_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAP8778165.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6563..7240)
                     /locus_tag="IV247_002106"
     CDS             complement(6563..7240)
                     /locus_tag="IV247_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAP8778166.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7277..8662)
                     /locus_tag="IV247_002107"
     CDS             complement(7277..8662)
                     /locus_tag="IV247_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAP8778167.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8872..8956)
                     /gene="ffs"
                     /locus_tag="IV247_002108"
     ncRNA           complement(8872..8956)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IV247_002108"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8979..9494)
                     /gene="tadA"
                     /locus_tag="IV247_002109"
     CDS             complement(8979..9494)
                     /gene="tadA"
                     /locus_tag="IV247_002109"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAP8778168.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9601..10458
                     /locus_tag="IV247_002110"
     CDS             9601..10458
                     /locus_tag="IV247_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAP8778169.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDGTEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10539..10874)
                     /locus_tag="IV247_002111"
     CDS             complement(10539..10874)
                     /locus_tag="IV247_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778170.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10941..12311)
                     /locus_tag="IV247_002112"
     CDS             complement(10941..12311)
                     /locus_tag="IV247_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAP8778171.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12389..12925)
                     /locus_tag="IV247_002113"
     CDS             complement(12389..12925)
                     /locus_tag="IV247_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8778172.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            13234..14715
                     /locus_tag="IV247_002114"
     CDS             13234..14715
                     /locus_tag="IV247_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8778173.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4526 a         2925 c         2394 g         4892 t
ORIGIN      
        1 gagtgtttcc cgattcgttt ttggctgccg tagcaacttt tatatcatat cagagagaca
       61 agcgattgtc aactcttttt taaaagtttt tttcgatttc gtttttcgaa atctttcagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcgt
      241 tagtaacatg tcaataacta ttttttaaat tttttagaaa tgtgatataa catccacttc
      301 agaatcaacg ctgttaaatt tatcatgtat ttcttccttg gtcaactata aattaagcat
      361 tactttgtga aatgattttt cgatcaatta ttattattcg ttatacaacc gttttcaaca
      421 tattttgaaa ataaaatgca ctaaaaaact atctttagca gaacatttca ttgtattttt
      481 tggaccaaac aataaaaaaa gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc
      541 tgtattttag aagaaaggca ctccctctac ttcgtaagtt ccaagaacag agcgcatttg
      601 acctggttct tcccaaatat atccttcgcc taattcttct ctttgattct tttgttcagt
      661 ttcaaagaca aacaacggaa cattttcctc tttaatatgt gtatttgtca attccatcaa
      721 actaatcttg ctaacatcga tatgaactat ttcttttaag aaattaagta tattcgctaa
      781 accagtcttt ccttcagaaa gttccgctaa cgcaaattcc aacttgtcat caattgaatg
      841 gaccaaaaat tttttagtac catcctccaa atgcaacata attgtaccag caactctttt
      901 gcttgccaaa atttccacct cgatcaactt gttctatggc ttacataaaa attctagcac
      961 attttcagaa aaaatacact ttttatttct tattttcttt ccgttctagt tcagataatg
     1021 cttcttctat ccaaataggt aactgctccg caatagtggt aaatccgatc ttacgatttt
     1081 tattcgtgaa ataccttttt ctgcgatatg gaacagctgg atttgttttt tctaacgttc
     1141 ttaaagataa actgattttt ttggaatatt catcaatgtc gatgatctga actgttactg
     1201 gctgtccgac ttttaacaat gaatggatat ttttcgtata accggattgt acttctgaaa
     1261 catgaataag tccttgcgtc tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc
     1321 ctgtcacttt tccttcaatt atttgtccaa ttttataagt catctacttc cctctcttcg
     1381 catttatcat tatactgtaa atagctcaaa aaaagaagcg acgaaaatga ctggaggtct
     1441 taagatgatc gaatttgata aacagatttc aagaaaagat acagaaagtg taaaatggga
     1501 cgcaattgct gagacatatc aaatggatga cttacttcct ttgtgggtag cagatatgga
     1561 cttcttgtct cccggtgggg tagctaaagc gtttagtgaa tacatcaaac atggaatctt
     1621 tggttatgcc actctgtctg ataaactata tcaggctatc attcattggg aacgccaaca
     1681 ccatgctgtg gagttgacga aagaaaatat cgtttttaca agtggtgtgt taaccagttt
     1741 agctgcagca gtacaaactt ttacaaaacc aggcgattct attttgattc atgacccagt
     1801 ttatcctcca ttttcctcta ttatagaaac aaatcaacgt cacatcgttc gaagctcttt
     1861 gctagaaaaa aataatcatt tcatcatgga cttgtctgac atggagaaaa aaatcgaaga
     1921 aaacaatgta aaagcaatga ttttatgcaa tcctcacaat cctggagggc gtgtttggag
     1981 caaagaagaa ttgaaacagt taagcgaatt gtgtttaaaa catcaagtga ttctgtttag
     2041 tgacgagatc catcaagatt taactttatt cgatcataca tttacttcca tgctaacgat
     2101 tgatcctaat ttagatgatc tactagttgc ttttacttcc gcaaccaaga ctttcaatct
     2161 agctgcaatc aaaaattcaa tggtatttat caaaaatcca gagatgaaag ctgattttga
     2221 aaaacattta gtgatgaacc aacagcacga aatcaacact tttggactga ttggaaccca
     2281 agcggcttat gagacgggtg gagaatggtt aagccaactg attccttatc tagaaaaaaa
     2341 tgttcaaaca gttaatcagt tttttgaaca gtatcttccg aaagtgagaa taatgaaccc
     2401 tgaaggaact tacttaatgt ggttagattt taccgcctat atttcagacg accaagaatt
     2461 ggagaacact cttgttcgga aaggaaaagt cgtattaaat cctggcatca cgtttggtcc
     2521 ttccggtcat gggcatatgc gcttgaatat tgcttgtcca gaagaaactt tactggaagg
     2581 acttaatcga atcaagtatg ctttagagac aatcgattaa atatttattc aaaataaaag
     2641 gacgagacct tactaggttt cgtccttttt cataattact ccgtaccata ccaaactatt
     2701 ctgaaatttc aattgtttca atcactacat catgtacagg tcggtcttgt ggaccacgtt
     2761 gtacatttgc gatttcatct actgtgtcca tcccttctag tacatgtcca aagactgtat
     2821 gtcggaaatc tagccaagga gttccgccac ctttatacgc ttcaatgatt tctgatggga
     2881 atccagcacc ttctagttgt ccaagcatat tagctggcac atttgtgtta tttacaataa
     2941 agaattggct tccgtttgtg tttggacctg aatttgccat agataaagct ccgcgaaggt
     3001 tgaacagttc acgactgaat tcatcttcaa aactttcgcc ataaatactt tcgccaccca
     3061 ttcctgttcc agttggatcg ccgccttgga tcatgaaatc aggaataacg cggtgaaaaa
     3121 tcacgccatc ataataacct tttttggaaa gttcaatgaa attttgaacc gtcttcggtg
     3181 ctagttcagg gaataattga acagtaatgt caccacggtt tgttttgata acagcttttg
     3241 gtcctttttc actttttaaa tctagttgtg ggaatgccat attgtttcct cctaatattt
     3301 gcatacttca taataataat aacataatac ctaacctctc gtaaaaatac tagatttgtt
     3361 ttcttttatt tttcatcact agatacattc tataacttac cgtttcgatt tcgaattcaa
     3421 acatttttga gaagcattct caattaaatt ctgctatagt gtgaaaaggg aggaattttt
     3481 atggaaatct atgacattac aatcatcggt gcaggtcctg tagggatgtt tgccgctttt
     3541 tacgcaggaa tgcgccaagc taaaacaaaa atcattgaca gtctacctca acttggtggt
     3601 caattagcaa ctttgtatcc cgaaaaatat atttacgata ttcctgggta tccagcaata
     3661 aaagcaagtg aattaatcga tcaattagaa aaacaattaa ctacttttaa ccatacattc
     3721 catttaaaag aagaagttct ctcgcttact cgtgaagacg aagttatcga gattactaca
     3781 aataaaggca tccattattc caaagcagtc attttagctt taggaaatgg ttcattccag
     3841 ccaagaaaat tgaatttaga taatgctgaa tcatttgaaa accacggatt ggattatttc
     3901 gtgaatgatt tgatgaagta tgctggaaaa aaagtcgcta ttgctggagg tggtgattct
     3961 gctatcgatt ggacgttgat gttagagcct attgcaagcg aagtttactt gattcatcgt
     4021 cgcccggaat ttcgtgcgca cgaacatagt gtatcacgct tgaaatcttc atctgtcaac
     4081 ttgcttactc cctatctcat cgatggatta tcaggcaata atggggaact gacagatatc
     4141 cggttaaaaa aagtgaaaag tgacgagacg attgatttga tgattgattc tctgatcgtc
     4201 aattatggat tctcttctaa tttagaacat ctttcttctt ggggacttga tagtaccaga
     4261 aatgccatta ctgtaaaatc agatatgtct acttctattc caggcgttta tgcagctggt
     4321 gacatctgta cctatgaagg aaaagtgaaa ctgattgcta ctgggctcgg tgaagcacct
     4381 actgcagtga acaacgcttt acactatatt aatccaaagg aacgaactca gcctggtcac
     4441 agcacaagct tatacgataa aaatcttcgt cctagttgat tttttatatc tctttgcatt
     4501 tcaagttaaa cccgaaagca gtttcttgca tttttttcat gttccgttaa catgaaatag
     4561 agaaagaaaa aataaagggg gagcaataat atggtcgtca aaactgagac tttagaagaa
     4621 aaggcaagag aactattgaa ggaacgtggc gtaactgtag aagatatcgc acaacttgtc
     4681 atgtttttac aaaaagacta tatttctgat ttgactttgg cggattgtac tgaaagtgtc
     4741 aacagcgtgc taacaaagcg tgaagtacat aacgccatta tcactggaat ccaattagat
     4801 atattagcag aaaagaaaaa attgatgagt ccgttgcaac atattatcga agatgacgaa
     4861 ggcctttacg gtatcgatga gatcctagcc ttgtcaatcg tgaatgttta cggtacgatc
     4921 ggctttacga attatggtta tatcgacaag gtgaaacctg gtattttaaa agaactaaac
     4981 agccacgatg gcgttcatgt ccataccttc ttagatgata tcgtcggagc gatcgccgct
     5041 gctgctgcca gtcgattagc acacaaagaa ccagaaaaaa gaagtcgcct tactcaataa
     5101 ttcaacaaaa aaagaaagaa aacgtctatt tagacgagtt ctttcttttt tgtttgtttc
     5161 aagctatttt ttcctaagaa aagaaaaaca gcaaaaaata attctaataa tacaaaaaat
     5221 aagataaagc tatgcatgaa aaattcttct ggaagtacat tgatgattgg atcagtaacg
     5281 ggatcgaata accagtcatc gttgttgaaa aacgtttcat gaaaaaggat aaaaaagcga
     5341 tcaaatccta tcatcatgaa aaaaccgaaa acaactggca gtaacatgcc gatttgaaac
     5401 ggacgaatca accgccaaag ccgatcattc ttttgcaaat atttgataaa caaaatactc
     5461 gggatcaaag tgatgaaaaa cacagcataa tccactaaaa acaaaatttt cacttcataa
     5521 aagtgatggg ctccgctagc agaaactggg aaatctggca agctgaggat ggttttgaag
     5581 ggattattga gataattcat taggtgatca aaatttttca gtaacgtctc ttgatctaaa
     5641 gaagtataat ccaaaatttg caaatgtccg atatcaaata cgtatagtgg tcgaaagttg
     5701 atcgtcacac caactgccag actgatcagt gtcagaaata ggctgaacaa tccagcatat
     5761 tccatccact gccatctcga atttttcata ttagaagctc cattcatcca gtgattgttt
     5821 cacataggta gctgggatag gcaacccagg tacatcttct tcttttgtaa aacctgataa
     5881 aacgagcaaa gtatctatat tattgcgtat cccagcttgg atatctgttt catagttatc
     5941 tccaaccatg attacttctt ctttttgcag ccctaatact tcaacggctt tatccataat
     6001 gatggcttct ggttttccga tgtagatcgg cggcgtttgg gttgctgtct ctacaaaaga
     6061 gatgactgac cctgctccag gaagaaggcc gcgttcagta ggtatattct tatctgggtt
     6121 cgttccaatg aaagtagcac ctttttgaat agcaagtgtt gctttaacta ctttttcata
     6181 ggtcagataa ttatctaagc ctaccactac atagtcagga gcctcttctt cccatgtaaa
     6241 tccggcagct aaaatcaagt caatcaatcc tgcttctccg ataacataga cttttttccc
     6301 ttttccatct gctttcataa aatcgattgt tgccaaagta gctgtgtaga ctgtctctgg
     6361 agctacatga atgtcaaact catctgctag acgattagct actgtttctg gtgacttagt
     6421 cgtgttattg gtaacaaaca agaaaggtaa ttttcttttt tgcaattctt ccacaaatcg
     6481 tttaccggcc ggtatgggtt gtgtcccacg ataaatcgtt ccgtctaaat caattaaata
     6541 tcctttatag tccattcatc cactactctt ccctttttct gatcgtaaat gctgtttttg
     6601 ttttttctac ttgtttaggt tgttcttgtt tttttacttt ttcagaggtt tttgctgact
     6661 ctgtttttcg atttttaata accggttttt tgcgattaga tgcgactggt ccttttttct
     6721 catcgatata cgcacgatta tggttttttt tcttttttcg gcgctgagtt gtcttttctt
     6781 ttttaccgcc gattctctcg atgacaaaat acgcacaacc aaaattgcaa tattcgtaaa
     6841 gatagtcttc caaggaatcg attcgctgat ctggatgcgc ccgtcgatta tccgcttgga
     6901 aaaatccttt taggcgtagt tgttcataac cccaatctcc tacgatataa tcatatcggg
     6961 ctagtacatc actgtaacgt tctccaagct tttctgcatt aaatccttca cgataatcgg
     7021 acacaagccg ataatgtctt tcacctatca aaaaattact gtcatctaac agtgtcacta
     7081 gctcgccttt gactttttct gctttaggct cctcaacgat ttcttccaaa gcagctgtca
     7141 gttcttcggt gactgttgtt tcctgttcag ctgatgattt tttcttagca atttctgttt
     7201 tatgctcaac gttctgctgt tcattttgtt ttgcagacat gcgttcacca cctatctttt
     7261 ttattatacc ttgtttttat ttgattgggt agtgagcttt caagtaatct cctaccacac
     7321 ttcggataaa ttcagggaac aaaaattcat tttttccagc aatctcaatc gttggaaaaa
     7381 atggaacaaa catcaaatga tctacggtag taaaagtata atttttattc agatcgattg
     7441 gcttgttctt ccaaaagact tgatggttaa tagaatcata tgtgattcca ttatagacca
     7501 tctgcccaaa aatcttaccg cgaaatccca ttcccttcat aggatagtta cgtaaaaagt
     7561 tacgattttt ttctatttct aaaactaagc ggatcagatc atttccaccc aatgtgacat
     7621 ttaacaagtg catcggatgc ggcaacgcag tgtgaagctg atcctgattg acccgccctt
     7681 ttggaatttc agttaaaaac agaccactgt ttaatattgc tgcttccgtc tttcctcgtt
     7741 ctttcaccgc ctcaaaggct acttggataa acgaatgctc attgaagata tctagactca
     7801 gagaaaatgg caaatctgct acttcttttt cttttaataa ttcatgccca cgttctagat
     7861 aactatttat ttcttgttca tcttctgtaa aagctgtcat tgtttcggta gggatggctt
     7921 tggctgaatg cttaaggatt tttttctctt catcgatggt taaatggact tcaccgacat
     7981 actgcccgta ctttccagcg gctgctattt ggacatcatt gattacgtga ccttctctga
     8041 ataaatgatg ggtgtgcgat cctaaaataa catcaattgc tggtatttct tttgcaattt
     8101 ggaaatcatc ctgaatcccg agatgactca tcaaaactaa aacatccact tgtgaacgta
     8161 aatcttcgac taattctggc aaaatatcat aaggattccg tatgtcccag ccattcggat
     8221 tataagtaag cggaaatgga gcagtaaatg caattagtcc gattttcgtc tggtgtttcg
     8281 ttgtaatgat tttatatggt ttcgtccatg ccggtggctg gagcgtattt ttatcgaata
     8341 agttatccag gatgatatca aactcagcat gatcgtataa atgattcaac tcttctttgg
     8401 aatttcctac gccttcattg tttcctatcg tcgctgcatc ataaccaact tcattcatca
     8461 gcaagatatt ttcctgtcca ttcgttgctt ctgacaatgg atgccaacga tccacaaaat
     8521 cacctagatc tactgcaatc gttgtcgttt tttctttctt ttcgttttct cgtttacgtt
     8581 gatccaaaaa ccttctgatc cttggccaat tttctaaatg agaatgcaga tcattcgtat
     8641 gtaaaatcac tattttttcc atgtaccctc tcgcctctct ttatctttat cataccatag
     8701 gcagcttttt cttccattga aacaagctct atttcagagt ttttcctgcc ttgaagcaaa
     8761 ttgtataagt acaatattcg ataaatcagt ctggctaaaa aatcaggaac attcattttt
     8821 acagtactat aaaaataaaa aaaacagacc cgaaagtctg ttgtcgtttc attataatca
     8881 gacacatggc ggaacctgct tagtgctgct tccttccaga cctgacacgc ttcacaagcc
     8941 caccattgtc ctagcctttc ggcaaagaat agtatacctt atttttcatt cgttgaccag
     9001 tcttttttta atttcttttt tcgctggcgg agctttcgga aaaagtcagt caaaatcatc
     9061 ccacattcgt cttctaaaat gccaccttct acatatgctt ggtggttaaa ccgctcgtcc
     9121 tccaaaagat tcatcaaggt accggctgtt ccgccttttg gatcatacgc gccgaaataa
     9181 acttctgcta cacgagacaa aatcatcgcg ccgctgcaca taggacaagg ctctaaggta
     9241 acaaaaagtg cagcatcttc caagcgccag cttcccactt tttcacaggc ttccttgatt
     9301 gcaagcattt ctgcatgtgt agtagcatct tgcgtggttt ctctaaggtt atatcctctt
     9361 ccaatcactt ttccatccaa tacaaccact gctccaatcg gaacttcatg aagcatttct
     9421 gctttctttg cttctgccaa tgcaaggcgc atccattttt ctttttctac tgcatcaaga
     9481 tttgtattta ccaattaccc ttccctgcct ttgttttttc ttgttttact ttagcatatg
     9541 ataaaaaggc tgtaactaac ttttttttct atggtaaaat aaatacaaga ggtgaacaag
     9601 atgaaaatgg ataaattact tgctctttac ccacaagcac agaaaaaaaa ctctccagca
     9661 tcagaagcgg atatcctttc ttttgcaatt gatgatgctt ttatctggat caaacaagat
     9721 tcattatctc aacaagaaac cagccttttg aaagcgttat tccctgtaat caacgatcaa
     9781 aaaaaacacc catggtatcg ctatcttttt aaagatgctt cttgtgttca agaaaaaagc
     9841 tttcgtttga tccagcttca cgtcgaatca aaaggagaat tcttaaaaaa agaatggcaa
     9901 gatactattt tggaattatt tcgctattct gctgatttct ttttttatac agacacagat
     9961 gcgctcttag tcgaacaaat aaaccgtgat tatcttgatg gtactgaaat caacggtatt
    10021 tttctatctt tagatgctga ttttgatacg acaacgtctg tattcgttgg ttctttccat
    10081 tcacctggca ccgacctcgt ccctttgttt gctgaagaaa gaaaaatttt tttaacagag
    10141 caaaacaatc tttatactca cagccgtact ttttctatgc aagatgtggc tttacattac
    10201 tatacaaaag atactgtaaa agatagtaag ttgatcaaaa gttacataga actgatcaaa
    10261 caaaatgaaa tggaagtgat cattcaagaa ttatggaatg aattaggcaa tgtcagctct
    10321 gctgcaaaaa cactgtttct tcatcgtaat actctaaaat atcgaatcga aaaatttcaa
    10381 gaacagtctg gtttcaatct aaaaaaagcc aacgatttac tgttttgcta tcttttattg
    10441 cttcatgaac ataattaaag atagattaaa ggggttgtga caataatttt gtcacaaccc
    10501 cttatttcga atgaatagtg ttcaaaagct agtttctctt acatcatttt ttcaacaaaa
    10561 tgcattagcc ggtcttttac atcttcggtt gaatctttga ttgaatcacc ataaacagag
    10621 attttctttt ttcctgattt gatttttgct aacattccca tcaaagaatc caagtcacta
    10681 tctgaaagac catctacgat ttctagtaat ttttcatttc catcaaattt gtcatagacg
    10741 aattttttta ctttcgtccg agtagtcata tgatggatct tttccattac ttttcccgat
    10801 acaacgactg ctgtcgctac actggcagcg gctgctacgc ttaatccgat tgttactttt
    10861 gttgatgttt tcataagtat cagctccttt ttatctatct ccatcatagc atagacaaaa
    10921 aaataaacga actaatccac tcagtgaaat ctcaatcctt tagggaaaaa gttctttact
    10981 gttcctttta cttgtttccc aaatccgaca ggtatcttat ctaatactag caaaacccat
    11041 ccttgattcc catcacgttg aaatgtttct cctgctgtgt aagattgcca ttccttttga
    11101 gtgatcggca agcaaggtat attctctatc ttttttgtag caagcgctaa tgcatagcta
    11161 ggttcaaatc gattcttttt gaaatctcct aaatgcaaac ctgtacgtac gacttttaaa
    11221 ccatcgagag aaggggctaa ctcaggaact tcccaaagat ggtcgttgaa cactaaaagt
    11281 cttccagtag cttcataatg aaaatcattt gaaaactccg tccagagttt ttcctgctct
    11341 tttgtcattt gcactttact tttttttcgt gtcttctttt ctttatgcat ctgattttgt
    11401 ccatgaaaag tcagctttgc aacaaaatgt ccttcaccct gatctttatg aggccatata
    11461 cgtattgttt tttccaaacc agctacgctt ccccattcag aacgtcctga actaacggat
    11521 tgagtcaaag gaatctcctc aatcgttact ggataatttt ctacaagcca gctaatgatc
    11581 tcttcatttt cttctggtgc aaacgtacaa gtcgaataaa tcagttgtcc tttatttttt
    11641 agcattttta ttgctgaaga caaaatctct tgctgacgtt tttgacagta tagcggagat
    11701 tcttctgtcc actcttttat tgcatttgga tcttttcgga acattccttc tcctgaacaa
    11761 ggagcatcaa ccacgatccg atcaaaaaag cccgaaaaat gagggacaag ttctgcgggt
    11821 gcatgatttg tcactattgc gttcgaaact ccccatcgtt ctatattttc tgataaaatc
    11881 ttcgcacgtt tcggaaagat ctcattcgtc actaataacc ctttcccctt catttgagca
    11941 gctaattggg ttgatttacc accgggagcc gcacacaaat ccaaaacttt ttcaccgggt
    12001 tttgctgcag cagctgttcc aacgatcatt gcacttggtt cttggctata ttcataccca
    12061 gcttgatgga ggaaagactt tccattgaca gtgccaagaa atccctcatt gctgtaggga
    12121 gcgggttgta attcttcgct atggtaagtt tggaccatgt ccaatcctgc cggttttagt
    12181 ggattccacc gaaatccttt tttcacgctt ccttgttcaa gcgcagaaaa aaaatcggat
    12241 gcttcttcgc ctaaaagaag acgatatttt ttgataaatt gctgtggtaa cgttgtcgct
    12301 tcctccttca ttccaatgtt tcgttagaca ttttacaatc ttttttacga aaaataaagg
    12361 tggctgatct actttttcat gttgctgcct aaggtgactg ttcctttaag atattttcca
    12421 agtattcatc cgtcacctcg tttttatccc aaacagcgtt catcatttca agaaaatctt
    12481 gagaagaatc atctgagcgc aaacttccca atgcattaag ccataccaat tcaagcgact
    12541 tttcttcttt gatcttcgtg tgacctttga catatgagaa aaatggccgc ttaaatagcg
    12601 aatccatgtc taaatcagat tttgcaatga tttcacgaat actttcatgg atagaaaaaa
    12661 aatctttcaa taagtagaca atgcttaaaa catcatcgga actgcattca gctaagtagt
    12721 tttcaaaata aacagtgatt tcttctaata tcttctcttt taacccgctg attgatttga
    12781 aatgtgtata aatcgggtat gtagaacaac cgcagaactc tgctaactct cttgcagtaa
    12841 ttgacttcat tccatattct tccacgtata ccatactttt gaacagcaaa tattctttac
    12901 tataatttgt tactctcccc atcattctta cctccgtctc ttttcatcta cccataaata
    12961 aataacataa ataattctaa aaatattatt gataattatc aaaacatatt tagttatatc
    13021 cattaaaaat caatttttcc gccttaaaaa caagaatatc ttgacaaaat accctaggaa
    13081 ataaacaata gaataaacaa ggtaacaaaa aaattaaata acgttatata aaaaagaagg
    13141 cgacactctg tgagaataac acagactgaa atgactttcc actcacagtt tttgtctaac
    13201 tatatacaaa aacagaataa aaaggaggta tttatgctaa ttgaagcaag aaaaaaagag
    13261 ttacttgttc tacaaatggt cattcaaaaa aataattgtt ctttacaaga attatccaat
    13321 gacttacata ttccaaaacg gacaatcaaa gaactgatcc gaaaaataaa tgtatcactc
    13381 aagcaatttt tattgattga tgaatttatt tattctaatc ataaaggaga gatccaaacc
    13441 aaagaaaccg atcaaactaa aaagttagct gttttttcta agttaaaatt atgctaccta
    13501 aaagaatcca atcgttttaa ctatttgatg ttactaatca attatccaca aactgctgta
    13561 ccaaaaaaat atttacttga acaactttat atatcgccct cctatctcga aaaactgaca
    13621 aaacagctca atcattcctt aaaaaaattc catttacgga tcctatctgt aaaaaattgc
    13681 tgtactcttg aaggaaatga gttatctatc cgtctctatc tttacttttt tctttcagat
    13741 acatttggag gtaacgattg gccattttcc tcacttgaat taaatgcact aaaaatgaac
    13801 agtacagatg agcaaataga aaaaatttca aaacaagaaa aaaatatcta cttgctgtta
    13861 actttattca tattacgaaa caaacaacag gcatattttc caaaaacttc ttctgaaatc
    13921 actgaattga tggacttatt gcagcataca catgattatt cagaatactt tacttctttt
    13981 tcagtgagtc gcccttgtga agagacagaa acagagagag cctactttaa ttattttttc
    14041 tattgttttt ttccaaatca aatctctttt cgacaaaaac aggagattgg ttttttattt
    14101 tccacaagca aaaaccttat ttgtcgacaa gtcggtcaat tgatgactta cttaactcaa
    14161 cagtttcctc tcaaccattt agaaaataaa ataccaattt atttttattt tcttgtgttg
    14221 gcagttgttt ttttcgaact gttgggtgaa tccagcagta gtttttctac actttgcttt
    14281 ccagaagcca cgtccaatca ttctagttct tttatacagg aaaagaaatt aagagaaaaa
    14341 atcgatacaa ttttttcaaa aaatggggaa agaaaccaaa tcagtcaata ccttagtcaa
    14401 ttgatccaac acttactaca aacagaaacg aaagagcagt tactcatcca tttgcagata
    14461 accaaacagt ttacaggaaa ttatcatctt caaagccatt tgcagcagtt atttcgcaaa
    14521 gatgctgtta aaattaccgc atattcagaa catgctgatt tgatcattac agatcagcat
    14581 gagaaaattt cagatgaaac ggagactttc tatctggata caacgcacaa tacagtgtta
    14641 tttggcaagt taataaaagc ggttcatgaa ctgtatatga aaaaaggtta tgagaacaaa
    14701 acaaagagga tgtgacaaaa ttcttgtcac atcctct
//
LOCUS       DACDIG010000063        14214 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.063, whole genome shotgun sequence.
ACCESSION   DACDIG010000063 DACDIG010000000
VERSION     DACDIG010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.063"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..316)
                     /locus_tag="IV247_002115"
     CDS             complement(<1..316)
                     /locus_tag="IV247_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8778174.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLHKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKI"
     gene            complement(432..998)
                     /locus_tag="IV247_002116"
     CDS             complement(432..998)
                     /locus_tag="IV247_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778175.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWLLAVVVVFIAIGSQGGSD
                     DAKNTVAETTKESVTEVSSAESVAESTVVEEETETTETTIEEVTQEESVPREYRNALS
                     KAESYLGWAGMSEQGLREQLEFEEYPSDAIDYALANVDVDYNEQALAKAESYDDWASM
                     SDSQLYDQLIFEGFTNEQAQYALDNLPQ"
     gene            complement(1134..1787)
                     /locus_tag="IV247_002117"
     CDS             complement(1134..1787)
                     /locus_tag="IV247_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778176.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDTTSNQETKISKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQATAEPSPTVISSSQSTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFSKYGIQ"
     gene            complement(1837..2265)
                     /locus_tag="IV247_002118"
     CDS             complement(1837..2265)
                     /locus_tag="IV247_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP8778177.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRDRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKKIEELKNLYGLPESAYRFLI"
     gene            complement(2270..2644)
                     /locus_tag="IV247_002119"
     CDS             complement(2270..2644)
                     /locus_tag="IV247_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8778178.1"
                     /translation="MSLVTKIKELADEKHVTIAEVERQVGISNGQIRRWDKASPKSEN
                     LKKVADYFGVTTDYLLGNNNVPKWATKEEVVELDKLLDSNVNMSYGGETLTPEQIQRV
                     KDILIATFWDIVKEDKEKGKKM"
     gene            2948..3133
                     /locus_tag="IV247_002120"
     CDS             2948..3133
                     /locus_tag="IV247_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8778179.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            3146..3394
                     /locus_tag="IV247_002121"
     CDS             3146..3394
                     /locus_tag="IV247_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778180.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            complement(3697..4155)
                     /locus_tag="IV247_002122"
     CDS             complement(3697..4155)
                     /locus_tag="IV247_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778181.1"
                     /translation="MIKILSDYQVAITLIISISGFFLSLYNLFKDKRKITLSYFLVKH
                     DRSNRMILTGVIANPSKMPNSIIECTYLYNGKKINCSHYHANGFDMGSIHKSSLLSPI
                     PLAQAISPGYSVAFSEVLDMKDILPGEKLVMVIRTANYQKKFKLKLKDSF"
     gene            4323..4640
                     /locus_tag="IV247_002123"
     CDS             4323..4640
                     /locus_tag="IV247_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778182.1"
                     /translation="MLIATDTLDRIFLKDEYKLRNIDASGILVFDLYDNGKIGIYQAS
                     DIEETNLAFEQIDDSVELDLDEAILAFEQIAKLLKEAQKNGNSLPTQRVIYQSPGTGR
                     TIG"
     gene            4573..5052
                     /locus_tag="IV247_002124"
     CDS             4573..5052
                     /locus_tag="IV247_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HAP8778183.1"
                     /translation="MATLYQLSESYIKVLELAEQLDEEILRDTLDSINEAIEYKAENL
                     AKIVKEVEGKAELIDSEIKRLQERKTSLLNNAKSIKHYLQEEMEKTGKTKIKGELFNI
                     GIQNNPVSVNVINENLIPKGFFTPVPPKLDKKQLKEELKHGDIPGAELVQTKGLRIR"
     gene            5502..5933
                     /locus_tag="IV247_002125"
     CDS             5502..5933
                     /locus_tag="IV247_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778184.1"
                     /translation="MKNKLAKTTAIIGLALGSGVIGYAASNAFQDLDTIKANFNTVLQ
                     YGQTKSQRVSELESQLSNNTRTQEQLKAEIEQIKSDKQKEIEAKQREVEQKQQEIATK
                     QQEADSLRQQLSTVQNDKEQLEQRVSELRQYTDQKVGELGK"
     gene            5930..6256
                     /locus_tag="IV247_002126"
     CDS             5930..6256
                     /locus_tag="IV247_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP8778185.1"
                     /translation="MKELTIYDPNISWAKHTIKVSFMIWGYKGYVTYKVGGNTKGLSL
                     IAIDSDDLYDANFEDNPVNFRDLDEDWFSMELTNDKGDSTLVEDEFDRLGDYIVGVEI
                     IAHEPE"
     gene            6375..7061
                     /locus_tag="IV247_002127"
     CDS             6375..7061
                     /locus_tag="IV247_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012197638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8778186.1"
                     /translation="MQIKKATDIEQTKGTYLIYGAPGKGKTSTAKYFPGKTLILDIDR
                     TSKVLRGEKNVDIVYIDNEDTWNDWGNTLADLTTNYVGVYDNVVVDNVSELERCILSS
                     LGAEGKNNGVPSQGDYQYMQFRMVNSLRYMKNLDSNLIWTAWEEIDLWTDSDGSSYNI
                     ALPQINRKIRNNILGLCDVVGRLMVKEDDERGFILQATDSTYAKNQLDNRSGCKQSEL
                     ILDGIVRLPE"
     gene            7036..8385
                     /locus_tag="IV247_002128"
     CDS             7036..8385
                     /locus_tag="IV247_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP8778187.1"
                     /translation="MELYDYQNDLVDRARQAYVDGYKAPCIVSPCGSGKSVMVAEIAK
                     RTTRKGNRVLFLVHRRELIDQIKDTFQKMGVDNQLVNFGMVQTVVRHLKTIKKPQLII
                     TDENHHGLAASYRKIYEYYSDVPRLGFTATPIRLNGSGLGDVNDLLIEGVSAKWLIEN
                     HRLAPYEYYAPKLIDTAELKKASTGDFTKKSMDKAVKNTIYGDVLKHYKTLAEGEQAI
                     AYCHSIDASKHTAEIFNDAGYKATHIDAKTPKDERADIIEAFRNHEIKILCNVDLIGE
                     GFDVPDCSTVIMLRPTQSLSLYIQQSMRGMRYRPDKTSIIIDHVGNVNRFGLPDMDRE
                     WSLDTKKKTKTDSDLSIVQCAFCFGAYERPKGDNICPYCGELQPIEERKSELEIDESA
                     ELMKVGETKITLNFENNKYYNMTEDEAESIEDLYAIARAKGFKPGWAYMAAKRKGWL"
     gene            8376..8849
                     /locus_tag="IV247_002129"
     CDS             8376..8849
                     /locus_tag="IV247_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8778188.1"
                     /translation="MVVGMGGIFGKKAEDVQGSIINGVTIIGDTGKRDSSGNQIVIAR
                     NKNNEVFESSLSNIKRGVVNGTGEESGYVNLKKANEKYHVEGTYVKALSVGGTKGVNW
                     DKRRNKWKARLYFKGEYVLQKDFNTKEEAIQARLAAEEKYFKPILEKYENKKEND"
     gene            8851..9351
                     /locus_tag="IV247_002130"
     CDS             8851..9351
                     /locus_tag="IV247_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010050472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HAP8778189.1"
                     /translation="MTGFNLDFSKAQQGNEIKDGTYEVVVNKAVENATKSGAEFIDID
                     LIVRNDVDQPFQNKHIFAKIWKAKATGKYNEGMIMAIAQALQLEDGKSYNGFDELLAD
                     FVLKTASVRVKTEESNGYKNVNVKSWDKTNTRGVMNHQFKNGDEPSFGPERSRATTVR
                     NDNLPF"
     gene            9366..11678
                     /locus_tag="IV247_002131"
     CDS             9366..11678
                     /locus_tag="IV247_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAP8778190.1"
                     /translation="MYEKIPKELKNLKQWCVYKLVWDEKRQKHTKIPYNANNGHKAKS
                     NDESTWSDFQTALAAIDHYGMSGLGFFFKPPYFGIDIDNAEGEVERFKSGDIEENIIY
                     EFIESMRSYAEYSQSGTGIHIIARGELPGGRRRKGDVEMYQNGRFFVMTGNAASKYLE
                     ITEPNPKDIKRLYDRYVGDEKIIQFKEENPLMNTVDLPVEEIIKRAESSSQGARFKVF
                     MNGGWEAFYSSQSEADMAFANDLAFWTGRDFEKMDEIFRASAMIRSKYDQKRGSATYG
                     ESLLNKAIADTNSVYNPKRKSDFKIFIKDQEQPKEEKYYSYDDTGNADRFTDIYGTLV
                     KYSYIDKSWYYYDGKVWLQDNTGEVRKMIDTTVDIMGKEPLTIPEGADDETKEALMKA
                     KEKHVKRSRSNAGKNAMMDELKHRLSVLPEEFDKDKTLFNTQSGYLSLHDGLLHEHEI
                     DKMFTRVSNVEYTESVDCPMWEEFINQIFDNDQELIRYIQKCVGYSLTGSTREQCMFI
                     LYGHGSNGKSVFLEIISELMGNYAMTMQAQTIMVKQSQSSANSDIARLKGARLVTSSE
                     PNEGVRLDEGLVKQLTGGDKVTARHLYGKEFEFEPEFKLWLATNHKPIIRGTDDGIWR
                     RLNLIPFTVQIPDHKKDKNLKFKLQTELQGILKWAIDGCLLWQREGLEKPKSVVAASQ
                     DYRNEMDQIGTFIETCCETGPGLKISGGELYKVYREWASDNGEHTFTNTKFGREISKK
                     YSKEKAGGFMVYKGITLKPRKYDNVRELFK"
     gene            11925..12242
                     /locus_tag="IV247_002132"
     CDS             11925..12242
                     /locus_tag="IV247_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HAP8778191.1"
                     /translation="MKSEHQIQDEIRIALSKHGCTIFRSNAGQVTTIEGRQFYGMPKG
                     FPDLCGHRNSDGKAIYIEVKNERGKLREDQKRFAEFLNGQPVLYGVARSVEEALKIVE
                     ESS"
     gene            12239..12400
                     /locus_tag="IV247_002133"
     CDS             12239..12400
                     /locus_tag="IV247_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP8778192.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            12397..12702
                     /locus_tag="IV247_002134"
     CDS             12397..12702
                     /locus_tag="IV247_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778193.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWSEKTK
                     RRTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRAKRENVDSKGRQFKHWEKK"
     gene            12702..13016
                     /locus_tag="IV247_002135"
     CDS             12702..13016
                     /locus_tag="IV247_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778194.1"
                     /translation="MEELITKVEQWAKDKGLDQADSSKQMLKTIEEIGEVAASLARKD
                     EYGLRDGIGDVVVTLIILAMQNDMDLYECLNQAYNEIKGRTGKMVDGVFVKSSDLEDS
                     DE"
     gene            13009..13539
                     /locus_tag="IV247_002136"
     CDS             13009..13539
                     /locus_tag="IV247_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAP8778195.1"
                     /translation="MNKKVLIDKQALIDELMKIPGVGSNSDALETIKRFPSYEPQKPV
                     VPKFVAELLDYYRQSTDVDLLALLITFHDWYYRKTKDGEHEEAIDWLVDHPEIYMRAW
                     LFGYELEKEPLYCVIINGRYLVKVFSNTNVVILVPADEFSQYVTQKFQLTEKQIKAID
                     ERYWPFAVPVDEVVEG"
     gene            13536..13805
                     /locus_tag="IV247_002137"
     CDS             13536..13805
                     /locus_tag="IV247_002137"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778196.1"
                     /translation="MSDPYITKLEKLSEDFEKNKNKLTKDKLIELVRLVIRGAITREK
                     AQQNIEIFARSVRDGLEKKVKQNEQQTPQNNKTKKTGTECTKDKV"
     gene            13935..>14214
                     /locus_tag="IV247_002138"
     CDS             13935..>14214
                     /locus_tag="IV247_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778197.1"
                     /translation="MKLKDGFYSSSHGIGGLMLDMPTKNPKTREKPKFKVGDMVRCEA
                     EGFIYPFCGYVEHLYNHSAIIRIENTMECDKWLAKSKENLAVARLVDME"
BASE COUNT         4835 a         2372 c         2994 g         4013 t
ORIGIN      
        1 gtatcttctt ctttatagtg tttaatatat tattcgtagg ataatatgta gattccttaa
       61 cttgtctttt gtatataact aaccattctt caacaagctc atgaaatgtt agatcaggct
      121 tatgaagtgt tttttcgttt aattttatat caattttttt atttaactct agcaatgcca
      181 ctttctgagt ttctcgagat ttatttttat aagtaatgct cacttttttt ctttttctag
      241 tttttggatc aatatatcgc tcattatatt tatatacttt ttttccgttt ttatcagttt
      301 tagtttcgat ccacattgtt ttcatctcct atttgttgct ataataggaa tagataagta
      361 agccgattat agcaggttta tttttcatca cgtccacaaa ctttggcgag ggaggggcgt
      421 gtttttgtta gttattgtgg taggttatct aaagcgtatt gtgcttgctc atttgtaaaa
      481 ccttcaaata taagttgatc gtacaattgc gaatctgaca ttgatgccca atcatcgtaa
      541 ctttccgctt tagccaaagc ttgttcgttg taatcgacat caacattagc cagcgcataa
      601 tcgattgcat cacttggata ttcttcaaac tctagttgtt cacgcaaacc ttgttcagac
      661 ataccagccc aacctagata gctttcagct ttgctcaatg catttctgta ttcacgagga
      721 acactttctt cttgagtaac ttcttctata gtagtttcag tagtttcagt ttcttcttca
      781 acgactgtag attctgctac cgattctgca gaagacactt ctgtcacgct ttctttagtt
      841 gtttcagcga cggtattttt agcatcatca ctgcctcctt gcgaacctat agcaataaac
      901 accacaacaa ccgctaacaa ccaaaaccat acccgtttat aaaacggttt cttaacttta
      961 tacattttcc catcttgacc cataactttt tttgccattt aaatatccct cgcttcttgt
     1021 tataatatat ttgtgatctc agaaatgagg tatgagtccg tgttgcagca cgggcttttt
     1081 tctttataac ttttttagag attataggca aaatagtagg gcataaaaat gtattattga
     1141 attccgtatt tagaaaatcc taattgaact tcaccggaag tcttttgctg tgttgtacgc
     1201 aatgcttctt cttcagacat tccattctgt actttccatg caacaggcga catcccgtat
     1261 ttgttaacaa aatcagtaag tgataaagtg tcagcgtctt gctgagcgct tgtttgttgg
     1321 tcttctggat tctgttgaga tgctgcttgt tgttctttct gatcttgact gataatattg
     1381 ccagcatcat ctgtagtcaa tccattttca taaagggcca cgccgaaagc ttcccactct
     1441 ttgttggacc aatttgcacg atcagctgga gttgactgta aagtgcgttg tttcatctgt
     1501 tcatatgttt cttcttgagg tgcggtttgg attgtactct gactggagct tataactgtt
     1561 gggctaggtt ccgccgtagc ttggttggag cttgtaactg ttgaactaga atctgtttta
     1621 gatgtagatt tgctagtaga tgaactggtt tcagttgttt cttttgtttt acttattttt
     1681 gtttcttggt tagaagtggt atctgttgat tcagcttttt tattatttga acaagctgaa
     1741 agtagcagag cagtacttaa caacaacata acgctaactt ttttcatttt ataattcctc
     1801 tttctcgttg taatatgtgt gctaacacgg gcttttttat ataagaaaac gataagcgct
     1861 ttctggaagc ccgtaaagat tctttaattc ctcgattttt ttaggatatt gatcattatc
     1921 ttctttataa agagaaacaa tgagattagc agcaaagcaa ttagcttcgc tttcagattt
     1981 gcttctagat gttcttgttg atacataata gctggataag ccacgatgaa aaatagcgtg
     2041 acctaattcg tgagcgcaaa tgtagaatct ttcctcagag tctcgcagtt catcatttaa
     2101 aaagattatc gcacgatctc taatttcttg aaactgcccc ttgggattct cgataaaagg
     2161 aacgtattga attttaatgc ccatcttttc acaaatataa aaaggattag cggactggta
     2221 tttccgcttc aactcctcga ctaaattaat cgtatccatc tccataagct cacatctttt
     2281 tgcctttttc tttgtcttct ttcacaatat cccagaaagt cgctatcagg atatctttta
     2341 cgcgctgtat ttgttcgggt gtcaatgttt ccccaccata agacatatta acatttgagt
     2401 ctagtagttt atcaagttca accacttcct cttttgtagc ccacttggga acattattat
     2461 ttcccaataa ataatcagtt gtgacaccaa aataatcagc aactttcttt aagttctcag
     2521 attttggcga ggctttatcc catcttctta tttgtccatt agagatgccc acctgtcttt
     2581 ctacttctgc tatagtcaca tgcttttcgt ctgctaattc tttaatctta gtaactaaac
     2641 tcattattat caacctttca aagctgaaaa gaaaataaat agcttaaaag ttatattttt
     2701 ggttgacaat tagcttttaa gctagtatat ttaattcgta agctaaattg ttagctaaat
     2761 aagagcaaca aaaaactcta ctaatttaaa acattctctc ggtcgccaaa cttagaaatg
     2821 ttattttaga ggctttttat aagtcttatt taactatgta ttcataatag cttaaaagct
     2881 aataggtgtc aacgatttag ctaatttttt agcttacaaa ttatttgttt agaaaggagc
     2941 tatttttatg tctgagaatt tagacttaaa aattcgagcg gagatgagaa aaagaagaat
     3001 gactttcaaa gaactagctg cgcttatcgg tatttcagga gcttatttat cagatattct
     3061 aaacggcaat cgtgatggaa agaaagcaca acagcatatc gaaacagtga aaaaaatatt
     3121 ggacatccga tagggggggt aggcgatgac aaaactaaaa aaacaagatt ttgtaaaaaa
     3181 atataattat tctccatcta cttatcaacg tcgaatgtcg gaactaaaaa atacagcaat
     3241 tttctcagcg gcgtatgaac gggtcacagg acaagaagtt tggatcaata cagaattata
     3301 cgataaattt ttgtctttca aatcctataa caggttacgc acaagaaagg taacacctaa
     3361 agaatttatc gagaagcatt tagttgattt ataaaaaaca gaacattttg agaggtgaag
     3421 gttaatgggt aaattcaaca gagcattagt attcagcgca ccgctaatcg tctatgcttt
     3481 agggctttgg ggaagtagac aagcgttgat aggaacgatt gtgtacatgg tttggatttt
     3541 tatagggctg gatgaagctg agtacaaaac aaaaaagcca gtcgggaggg actgactaat
     3601 gagtaaggac gatattgtat ttctgttaat tatgttttgt gggattggat caataataat
     3661 taatttgtat ttgattatta aagaaaaatt tttcaattaa aatgagtctt ttagctttaa
     3721 cttaaatttt ttttgataat tagctgttct tatgaccatt acaagttttt caccaggtaa
     3781 tatatctttc atatctaaaa cttctgaaaa agcgactgag tagccaggag agattgcttg
     3841 tgccaacggt ataggagata aaaggctact tttatgaatg cttcccatat caaaaccatt
     3901 ggcgtgataa tgtgaacaat taattttctt accgttgtaa agatacgtac attcgataat
     3961 tgagtttggc atttttgatg ggtttgcaat tacgccagta agaatcattc gattacttct
     4021 atcatgctta actaaaaaat aggagagcgt aatttttctt ttatctttaa ataagttata
     4081 gagggataaa aaaaatcctg aaatagaaat gattaaagta atagcgactt ggtaatcgct
     4141 caaaattttt atcaagtaat tcaccacctt tttactcaaa ttatatcaaa aaggagagaa
     4201 gaaataatgc aagaattagt aattttgaaa aataaaaaaa gacacgaacg ccggcaagca
     4261 tgatcgtgtc agggaaataa actatctttc tatattttac cacagaaagg aatgaaccgt
     4321 aaatgctaat tgcaacggat acactagacc gcatcttttt aaaagacgaa tacaaactgc
     4381 gcaatataga tgcgtcagga attttagtat ttgatcttta tgacaatgga aaaattggta
     4441 tctatcaagc aagtgatatt gaagaaacaa accttgcttt cgaacaaatt gatgattctg
     4501 tggaattgga tttagatgag gcaatcctag cttttgaaca aattgcaaaa ttattaaagg
     4561 aggcacaaaa gaatggcaac tctttaccaa ctcagcgagt catatatcaa agtcctggaa
     4621 ctggcagaac aattggatga agaaattctt cgcgatactc tcgattcgat taatgaagcg
     4681 atcgagtata aggcagaaaa cttagcaaaa atagttaaag aagtagaagg gaaagctgag
     4741 ttaatcgata gtgaaattaa acgtctgcag gaacgtaaga catcgctttt gaacaatgct
     4801 aagagtatca agcactattt acaagaggaa atggaaaaga ctggtaaaac gaagattaaa
     4861 ggtgaattat tcaacattgg aattcaaaat aatccagtat cggtcaatgt aatcaatgag
     4921 aacttaattc caaaaggatt ctttacccct gtgcctccca aattggataa aaagcaattg
     4981 aaggaggaac tgaagcacgg agatattcct ggtgctgaac tcgtccaaac aaaaggtttg
     5041 aggataagat gatgaaggag gagaaataat ggaacacgaa aacgtaaaag acgctttgaa
     5101 agctgctatc gaaatcgcgg aagctaaagg aataaaggtt gacggcaaac cagcaactgt
     5161 ccatgacatt caaaatttga cgaaagaaca tctttatttt atcgccgact tgcttggttt
     5221 gtcggaattg tatttagaca aataacccca cacggaagga gtggctaggg tggttgtatt
     5281 acaagtaata gtctgcgtaa cattcatttg gtcgtgcata ccggtttgta tatatatcat
     5341 gaagaagctt cgccctgagt cttacaaatt atacgcaatt tacagcataa cagtagctct
     5401 tgcatttaca ttgtttgcaa tttctaggct tccgttttaa tcgtgggaat gaatttttaa
     5461 tgaactaaat tatctataaa gcaaaaaaag gagaaacaac attgaaaaac aaactagcaa
     5521 aaacaacagc aatcatcgga ctagcactag gtagcggcgt tatcggctac gcagcaagca
     5581 atgcgtttca agatttggac acgatcaaag caaatttcaa cacagtccta caatacggtc
     5641 aaacgaaatc gcaacgtgtg tcagaacttg aatcacagct atccaacaac actcgcacac
     5701 aggagcagct gaaagccgaa attgagcaaa tcaaatcgga caagcagaag gaaattgaag
     5761 ccaagcaacg agaagtcgaa caaaagcaac aggagatcgc tacaaagcaa caggaagccg
     5821 atagcttgcg ccaacaacta tccacagtgc aaaacgacaa ggagcagtta gaacagcgcg
     5881 tgagcgagtt acggcagtat acggatcaaa aggtagggga gttggggaaa tgaaagagtt
     5941 gactatttat gacccaaata tttcatgggc taaacacaca attaaagttt cttttatgat
     6001 ttggggttac aaaggatatg ttacttacaa agtgggcggt aacactaagg gtttatcatt
     6061 aatagctatt gattcagatg atctatatga tgctaatttc gaggataacc cagttaattt
     6121 ccgtgacctt gatgaagatt ggttctctat ggaacttact aacgataaag gcgacagtac
     6181 acttgtagaa gatgaattcg acagactagg tgattatatt gtcggtgttg aaattattgc
     6241 gcacgaacca gagtaggaga gtgaatacaa aatgattaaa agaatcacta tatcagttga
     6301 agaagatagt ggatcaacaa tgcagcatac cgacaatcga aaaacttttt taaccaatag
     6361 aaaggagaat tttgatgcag attaagaagg cgacggatat cgaacaaacc aagggaacat
     6421 atttgatcta cggtgctcca ggaaaaggta aaacttcaac ggctaaatat tttccaggga
     6481 aaacactgat ccttgatatt gatcggactt caaaagtcct tagaggtgag aaaaatgtcg
     6541 atatcgtata tatcgacaac gaagatacct ggaacgactg gggcaacacg ttggctgatt
     6601 taactaccaa ttatgtaggt gtttatgaca acgtggttgt cgataatgtc agcgaattgg
     6661 aacggtgtat tctgtccagt ttaggcgcag aaggaaaaaa caacggcgtt ccttcgcaag
     6721 gcgattatca atatatgcaa tttagaatgg tgaacagcct acgctatatg aaaaatttag
     6781 acagcaacct tatctggacc gcatgggaag aaatcgattt gtggacggat tcggatggat
     6841 cttcttacaa catcgctttg ccgcagatca atcgaaagat tcgaaacaat attttgggat
     6901 tatgtgacgt tgttggtcga ttgatggtaa aggaagacga tgaacgcgga tttattttac
     6961 aagccacaga ttcaacctat gccaaaaatc aattggataa tagatctggc tgtaagcaaa
     7021 gtgagctgat tttagatgga attgtacgac taccagaatg atttagtcga tcgtgcaaga
     7081 caagcctatg ttgatggtta caaggcacca tgtatcgtat caccgtgcgg ttctggaaaa
     7141 tcagtcatgg ttgcagagat cgctaaacga accacacgaa aaggcaacag agtgttgttc
     7201 ttggtccatc gtcgtgaatt gattgatcag atcaaagata cgtttcaaaa gatgggcgta
     7261 gataatcagc ttgtaaattt cggcatggtt caaacagtcg tacgacatct taaaaccatc
     7321 aagaaaccac aactgatcat cactgacgaa aaccatcacg ggttagcagc gtcctacaga
     7381 aaaatctatg agtattattc ggacgttccg cgattaggtt ttaccgcgac acccatccgg
     7441 ttaaacggta gtgggttggg ggatgtgaac gacttgctaa ttgaaggagt atcggctaaa
     7501 tggttgattg agaatcatag attagctcct tatgaatatt atgcacctaa attgatcgat
     7561 acagccgaat taaagaaagc ttcgactgga gattttacaa agaaatcaat ggataaggca
     7621 gtgaagaata ctatttatgg cgacgtgttg aaacattata aaacgttagc agaaggtgaa
     7681 caggcaatag catactgtca cagtatcgat gcaagtaaac acactgctga aatattcaac
     7741 gatgctggtt ataaagcaac acatatcgat gcaaaaactc caaaagatga acgtgcagat
     7801 attatcgaag cgttcagaaa tcatgaaatt aaaatcctat gcaatgttga tttgatcgga
     7861 gaagggtttg atgttcctga ttgttcgact gtaattatgt tgagacctac tcaatcgtta
     7921 tcattgtaca ttcagcaatc gatgaggggg atgcgttacc gaccagataa aacgtcaatc
     7981 atcattgacc atgtgggtaa tgtgaaccgt tttggattgc ctgatatgga tcgtgaatgg
     8041 agtttagata caaaaaagaa aacaaaaact gatagtgatt tatctatcgt tcaatgcgcg
     8101 ttttgttttg gtgcttacga acggccaaaa ggcgataata tttgtccgta ttgcggagag
     8161 ttgcagccga ttgaagaacg aaagtcagag ttagaaatcg atgaatcggc tgaattaatg
     8221 aaagtcggag aaacgaaaat tactttgaat tttgagaaca ataaatatta caacatgacg
     8281 gaagatgaag cagaaagtat agaggactta tatgccatag caagagctaa aggatttaaa
     8341 cctggttggg cttatatggc tgctaagagg aaggggtggt tgtaggaatg ggcggtattt
     8401 ttggtaaaaa agcagaagat gttcaaggtt caataataaa tggcgtaaca ataataggtg
     8461 atactggaaa aagagatagt tcaggcaatc aaattgtaat cgctagaaat aaaaataacg
     8521 aagtttttga atcttctcta tctaatataa aaagaggtgt tgttaacggg acaggcgaag
     8581 agagtggcta cgttaattta aaaaaagcta atgaaaaata ccatgttgaa ggaacttacg
     8641 ttaaggcttt gtctgtcggt ggaacaaaag gcgttaattg ggataaacga agaaataaat
     8701 ggaaagcacg attatatttc aaaggagaat atgtcttaca aaaggatttc aacaccaaag
     8761 aagaagcaat ccaagcacga cttgcagcag aagaaaaata tttcaaacct atcttagaaa
     8821 aatatgaaaa taaaaaggag aatgattaac atgacaggat ttaacttaga cttttcaaaa
     8881 gcgcaacaag ggaacgaaat caaagacggt acgtacgaag tggtcgtaaa caaagctgta
     8941 gaaaatgcaa ctaaatcagg cgcagaattt atcgatatcg atttgatcgt tcgtaacgat
     9001 gtggatcaac cgttccaaaa taaacacatt ttcgcaaaaa tctggaaagc caaagctacc
     9061 ggcaaatata acgaaggtat gatcatggca attgcacaag cgctacagtt ggaagacgga
     9121 aaatcatata acgggtttga tgaattgctg gcggactttg ttttgaaaac cgcctcagta
     9181 cgcgtgaaaa ctgaagagtc aaatggatat aaaaacgtca atgtgaagtc ttgggataaa
     9241 acgaacacac ggggcgtaat gaatcaccaa ttcaaaaatg gtgatgaacc atccttcgga
     9301 ccggaacgtt caagagcgac aacggttaga aatgacaatc ttccatttta attgaggtgt
     9361 aatcaatgta tgaaaaaatt ccaaaagagc ttaaaaatct caaacaatgg tgtgtttata
     9421 agctagtttg ggatgaaaag cggcagaagc acacgaagat cccttataac gccaacaacg
     9481 ggcataaagc caaatccaat gacgagagta cctggtctga ttttcagacc gctctcgctg
     9541 ctattgatca ctacggaatg agtggactgg ggttcttttt caaaccacct tatttcggca
     9601 ttgatatcga taatgctgaa ggtgaagttg agcgatttaa atcaggtgat atagaagaaa
     9661 atattatcta tgaatttatc gaaagcatga gatcctatgc tgagtattct caatcaggta
     9721 ctggcatcca cattattgct cgcggagaat tgcccggcgg aagacgcagg aaaggtgacg
     9781 tagaaatgta ccagaacggc cgattcttcg ttatgactgg aaatgccgca tcaaaatatt
     9841 tagaaataac tgagccgaat ccaaaggata tcaaacgcct atatgatcgc tacgttggag
     9901 atgaaaaaat catccaattc aaagaagaaa atccgctgat gaataccgtt gatttaccgg
     9961 ttgaagaaat tatcaaacgt gctgaatcgt cttcccaagg ggcaagattc aaagtcttta
    10021 tgaacggcgg ttgggaagct ttttattcct cgcaatccga agcggatatg gcgttcgcca
    10081 acgacttggc tttttggacc ggtagagact ttgaaaaaat ggatgagatt ttcagagcgt
    10141 ctgcaatgat ccgtagcaaa tatgaccaaa aaaggggatc ggcaacttac ggagaatctc
    10201 tactaaataa ggcaattgct gatacaaatt ccgtctacaa ccccaaacgg aaatctgatt
    10261 ttaagatttt tatcaaagac caggaacagc caaaagagga aaaatactat tcgtacgacg
    10321 acaccggcaa cgcggatcgg tttactgata tctacggaac gttagtcaaa tattcgtaca
    10381 ttgataaatc ttggtattac tacgatggga aagtttggct acaggacaat accggcgaag
    10441 ttcggaaaat gatcgatacg acagtagaca tcatgggaaa agaaccgtta acgatccctg
    10501 aaggcgcaga cgatgaaacc aaagaagctt tgatgaaagc gaaagaaaaa cacgtcaagc
    10561 gttctcgcag caatgccgga aaaaacgcta tgatggacga attgaaacat cgactatccg
    10621 tattgccgga agagttcgac aaagataaaa cgctgttcaa cacacaaagc ggctatctgt
    10681 ctttgcatga cggtcttcta cacgagcatg agatagacaa aatgtttaca cgtgtttcaa
    10741 atgtcgagta cacagaatcg gtcgattgtc ctatgtggga agaatttatt aatcagattt
    10801 ttgacaatga tcaagaatta atcagatata tccagaaatg tgtcggatat tccctgaccg
    10861 gatctaccag agagcaatgt atgttcatcc tctacggtca tgggtcaaat ggaaagtcgg
    10921 tatttttgga aattatttct gaattgatgg gaaactatgc gatgactatg caggcacaaa
    10981 cgatcatggt caagcaaagc caatcatcag caaacagtga tattgcacga ttgaaaggtg
    11041 cgcggttggt cacttcgtca gaaccgaatg aaggcgtccg actggacgaa ggtctggtca
    11101 aacaattgac tggtggtgat aaagtcacgg ctcggcacct ttatggaaaa gaattcgagt
    11161 tcgaacctga attcaagttg tggctggcaa cgaaccacaa gcccatcatc cgtgggaccg
    11221 atgacggcat ctggcgacgg ttaaatttaa ttccattcac tgtccaaatc ccggatcaca
    11281 aaaaggataa aaatctgaaa tttaaattgc agacggagtt gcaagggatc cttaaatggg
    11341 caatcgatgg ttgcctgttg tggcagagag agggcttgga gaaaccaaag tcggttgttg
    11401 cagcgagtca ggattatcga aacgaaatgg atcaaatagg tacatttatc gaaacgtgtt
    11461 gcgaaacagg tccaggacta aaaatatcag gcggagaatt atacaaagtt tatcgtgaat
    11521 gggcatcaga taatggcgag catactttca ctaacacgaa gtttggcaga gagatttcaa
    11581 agaaatatag caaagaaaaa gctggtggat ttatggttta taaaggaatc acattaaaac
    11641 caagaaaata cgataacgtt agagaacttt ttaaataaat ctggagggtt ctggagggtt
    11701 gagaacgaaa ccctccaacc cttagaaacg ttgatatatc agtatttata ttatattttt
    11761 ctttctttct ggagggtttt aaaaaaataa taaagtatta aagtaaaaaa taaaaagtaa
    11821 attatataaa aaagaatagg tttttgggtc tgaccctcca aactctccac caaaatcttc
    11881 ctaaatgctt tgataccaac gattaaacag tctggagggt tacattgaaa tcagaacatc
    11941 aaatacaaga cgagatcaga attgccttat caaaacatgg ttgcacgatt tttcgatcca
    12001 atgcgggaca agtgacaacg atcgaagggc ggcaatttta cgggatgcca aaagggttcc
    12061 cagatctatg tggacatcgt aattctgatg ggaaagcaat ttatatagaa gtaaaaaatg
    12121 aacgcggtaa gttgcgtgaa gatcaaaaaa ggtttgctga atttttaaac ggtcaacctg
    12181 tactatacgg tgttgcacga tcagtggaag aagcattgaa aattgtggag gaatcatcat
    12241 gacaacagaa gaagtgattc aaatgcgtat tcgaagcctt cagcgtgaga ttgacgatct
    12301 ggaacggaca aaggcagtga tggtcaatga aacggcgagg aaggcaatcg atttgcacat
    12361 agagaattta agaagggaaa tccatcgatt ggaggaatga gcgtggataa gaaagcagca
    12421 atgaaacgaa tcatcgaact gacacattct gagaattggc aagaagacaa agaaatagtt
    12481 gcagaagtcc aaaagctcgg taaatcaatg tggagtgaaa aaaccaaacg gagaacaccg
    12541 agaaaaattg caatctggca tggtgaccga attctagtaa caggtactgc tgaacagtta
    12601 tctgaaatta ctggattaag caaaaacatt atctgggata gagctaaacg cgagaacgtt
    12661 gattcgaaag gtcgtcaatt taaacattgg gagaagaaat aatggaggaa ctaatcacaa
    12721 aagtagagca gtgggcaaaa gataagggat tggatcaagc tgattccagc aagcaaatgt
    12781 taaaaacgat cgaagagatt ggggaagttg ccgcttctct agctagaaaa gatgaatatg
    12841 gtttaagaga cggaattgga gacgtagtag taaccttgat tattttagct atgcaaaatg
    12901 atatggattt gtacgagtgt ctgaaccaag catacaacga aatcaaagga cgcacaggaa
    12961 aaatggtaga tggtgtattc gtgaagtcga gtgatttgga ggacagcgat gaataaaaaa
    13021 gtattaattg ataaacaagc attgattgat gaattaatga aaatacctgg tgtgggatct
    13081 aatagtgacg ctttagaaac gattaaacgt ttcccatcgt atgaaccgca gaagccagtt
    13141 gtgcctaagt ttgtggcgga actactcgac tattatcgtc agtcaacaga tgttgattta
    13201 ttagctttat tgataacttt ccatgattgg tattatcgca aaactaaaga cggtgagcat
    13261 gaagaagcaa ttgattggct agttgatcat cctgaaattt atatgcgtgc atggctgttt
    13321 ggctacgagc tcgagaaaga gccgttgtat tgcgtaataa tcaatggtag atatcttgtc
    13381 aaagtattca gcaatacaaa cgttgtcata ttagttccag cagatgaatt ttcacagtac
    13441 gttactcaaa aatttcaatt aactgaaaaa caaatcaaag caattgatga aagatactgg
    13501 ccgtttgctg tgccagtgga tgaggtggta gaaggatgag cgacccatac attactaaat
    13561 tagaaaagtt gtccgaagat tttgagaaga ataaaaataa actaactaaa gataaactta
    13621 tagaactggt taggttggtt atcagaggag ctattacaag agaaaaggcg cagcaaaaca
    13681 tagaaatttt tgctcgtagt gtccgagatg gattagagaa gaaagttaaa caaaatgaac
    13741 aacagacacc gcagaataac aaaactaaga aaacgggaac tgaatgtact aaagacaaag
    13801 tttgaaaaag aatatggaat ttcagcagaa gaaacatata aagtggcaag tcagtgtgtt
    13861 gctgatgcga gcggcgctat tcgtaagttt ggaatttcga tattaagtga tgatcgtaaa
    13921 tgggaggaaa aagaatgaaa ctaaaagacg gattttactc cagcagtcat ggtatcggcg
    13981 gtttaatgct agatatgccg acaaagaacc ctaaaacacg tgagaaacca aaattcaaag
    14041 tcggtgacat ggttcgctgt gaagcagaag gattcatcta tccattttgt ggatatgtag
    14101 aacatctcta taatcactca gcaatcattc gtattgaaaa cacgatggaa tgtgacaagt
    14161 ggttagcgaa aagcaaagag aatttagcag tagcgagatt ggtggatatg gagg
//
LOCUS       DACDIG010000064        14127 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.064, whole genome shotgun sequence.
ACCESSION   DACDIG010000064 DACDIG010000000
VERSION     DACDIG010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14127
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.064"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..400)
                     /locus_tag="IV247_002139"
     CDS             complement(<1..400)
                     /locus_tag="IV247_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778198.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLN"
     gene            complement(393..794)
                     /locus_tag="IV247_002140"
     CDS             complement(393..794)
                     /locus_tag="IV247_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778199.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            complement(784..1137)
                     /locus_tag="IV247_002141"
     CDS             complement(784..1137)
                     /locus_tag="IV247_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP8778200.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            complement(1127..1438)
                     /locus_tag="IV247_002142"
     CDS             complement(1127..1438)
                     /locus_tag="IV247_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778201.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            complement(1435..2262)
                     /locus_tag="IV247_002143"
     CDS             complement(1435..2262)
                     /locus_tag="IV247_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778202.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLSFVNKKKCSYAYVKAIKAVVR"
     gene            complement(2272..3432)
                     /locus_tag="IV247_002144"
     CDS             complement(2272..3432)
                     /locus_tag="IV247_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP8778203.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            complement(3432..4118)
                     /locus_tag="IV247_002145"
     CDS             complement(3432..4118)
                     /locus_tag="IV247_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAP8778204.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            complement(4081..5259)
                     /locus_tag="IV247_002146"
     CDS             complement(4081..5259)
                     /locus_tag="IV247_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP8778205.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            complement(5279..6973)
                     /locus_tag="IV247_002147"
     CDS             complement(5279..6973)
                     /locus_tag="IV247_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP8778206.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            complement(6951..7265)
                     /locus_tag="IV247_002148"
     CDS             complement(6951..7265)
                     /locus_tag="IV247_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAP8778207.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            complement(7368..7649)
                     /locus_tag="IV247_002149"
     CDS             complement(7368..7649)
                     /locus_tag="IV247_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778208.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            complement(7654..7998)
                     /locus_tag="IV247_002150"
     CDS             complement(7654..7998)
                     /locus_tag="IV247_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP8778209.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            complement(9185..9652)
                     /locus_tag="IV247_002151"
     CDS             complement(9185..9652)
                     /locus_tag="IV247_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HAP8778210.1"
                     /translation="MVLFDVKKYETPEAKDVDMDRTKHNVGVFLSAYLSARCRIGQPR
                     EPKVTASYSLVPSSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKLESNKIIIQFASSLELVAFK"
     gene            complement(10244..10531)
                     /locus_tag="IV247_002152"
     CDS             complement(10244..10531)
                     /locus_tag="IV247_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778211.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKEPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAVIRIENTMDCDKELAKSKANIAVVRLVDMKAI"
     gene            complement(10531..10725)
                     /locus_tag="IV247_002153"
     CDS             complement(10531..10725)
                     /locus_tag="IV247_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778212.1"
                     /translation="MKAYTIKFYGNKTFVYILSGLRLDFRGYSKDDLLESLYGYGYLN
                     EDELISLEKFTNAWTIEEEA"
     gene            complement(10728..11114)
                     /locus_tag="IV247_002154"
     CDS             complement(10728..11114)
                     /locus_tag="IV247_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778213.1"
                     /translation="MKSLQQLISEKESELQNLEDSLGLGFPIVEQAKMTQISHLRLES
                     EDLRQIEKSIQLNDNQQIVFDFLKNEFKRNDLQYTLWSFTEDVYERLEIGVSSLPYVK
                     AWGKLKEKQQFEVLTAFAQWGLEQEE"
     gene            complement(11111..11650)
                     /locus_tag="IV247_002155"
     CDS             complement(11111..11650)
                     /locus_tag="IV247_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAP8778214.1"
                     /translation="MNKQELIDKLASLVGSIEDFKGISTFHDGKYAGLEHALELIKQL
                     DEPQKPFVPKFVADYIEEYRGGGATLYEMMDSSEDEVYTWLFGNPRIKTENERQLLFA
                     QAYAVGYEVEKEPLYEVIIGNLFLVKKFNNRNDSCFVNKFELCWWEKSAYQLTEPEIK
                     AIDERYWPFAVPVEEVAEG"
     gene            complement(11643..11963)
                     /locus_tag="IV247_002156"
     CDS             complement(11643..11963)
                     /locus_tag="IV247_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017547333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAP8778215.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILDEYME
                     DSDE"
     gene            complement(11963..12262)
                     /locus_tag="IV247_002157"
     CDS             complement(11963..12262)
                     /locus_tag="IV247_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778216.1"
                     /translation="MDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            complement(12265..12426)
                     /locus_tag="IV247_002158"
     CDS             complement(12265..12426)
                     /locus_tag="IV247_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP8778217.1"
                     /translation="MTTEEVIQMRIRSIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEG"
     gene            complement(12423..12725)
                     /locus_tag="IV247_002159"
     CDS             complement(12423..12725)
                     /locus_tag="IV247_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778218.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            complement(12887..13156)
                     /locus_tag="IV247_002160"
     CDS             complement(12887..13156)
                     /locus_tag="IV247_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778219.1"
                     /translation="MKIDYLELINEIAKYKTGEEIEILRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            complement(13168..14019)
                     /locus_tag="IV247_002161"
     CDS             complement(13168..14019)
                     /locus_tag="IV247_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP8778220.1"
                     /translation="MADNKRYYYLKLKENFFDSDEMVLLESMPDGYIYSNILLKLYLR
                     SLKHEGKLMFNDRIPFNSTMLATITRHSVGVVEKAVQIFRDLQLIEVLDNGAIYMSDI
                     QSFIGKSSTEADRKRKYRKEIEEAKQNLITSGQMSDKCPDKTTPELEIELEKDIELEK
                     DIENVTPSKKSKAKPVRHKYGEYKNVLLSDEQMEKLKIEFPNDYQERIERLSEYCESS
                     GKTYKNYLATIRSWARKEKSESKNASSGYKRTGRREKLPEWAIDQEAYLKKKALERAN
                     RQSKAPF"
BASE COUNT         3896 a         2992 c         2095 g         5144 t
ORIGIN      
        1 tatttaattt atcgaattga cgaatcagaa gttctgtaat tggatcaagt gcaagaccta
       61 atcctcttct cataaattcg tccggctgat ttcttttaga agtacctatc cccaaatcag
      121 gatattttaa atactcaaat tttcttgtag gtctaatgat gaaacccaaa ttaatgtatt
      181 gagtcttaag tggacgacta ttttttgcgt gttggtgccc tcttcttaaa tctgcttcag
      241 aaacaggaat tttttctgta atcctatcca ctgcaatagc cgaacctttt gatttcaatg
      301 cttcgttaat cagtcgttcg ctctcgcttg aatagcgttc catccgcaca agaagttcat
      361 catgtccatt tatttttagc tcccaactat ttttagccat gacaatcact cttcaataat
      421 cgtctaaacg taaataccaa ttgatcgata tagcgatctt ggttctctag ttttaaatga
      481 ttgggatcca ttctctgaaa acgaatcgaa cgattttgaa tcaatgaaat aatatctagt
      541 gaatctcctg ttaaatcttc tctattttct gaatagaaag ttagatatag attttgaccc
      601 acgctatatt ttggctcagt gatcatttct atttctcctg tttcgagaat gaagtaatta
      661 aaatcatcag gtagctcatc ctcgcctacg gagtcttgaa agagtttgag gccaaaatgc
      721 tcttctaagg aagttttgat agcagaaatt tgcttattta aacgttcttt ttctttagaa
      781 ttatcaatca ccatattcac ccacactttc aagataaaaa tagatataaa aattatcgta
      841 atcggcatag ataacgttgt aacgcatact atcgattacg ataaaatatt gatctttatt
      901 aaatttcttg gcgattggat gaaatggagt ctttactttc ttagttaatt tcgatcccat
      961 cgcatccata gctgttatat cactatctct catggaaaga tttctaaatt ttaaagaagt
     1021 gatttctgta tcttctacac caatcttttt tcctagttca tttcttttgg tagtttgcgt
     1081 caaaatcttt aaccaaccat cgttgaatgt ttcttcgagt ctacgattat tcgccattca
     1141 catcacctgc aatatattct tgtagcgcat aatgttgaat gaaacctaat aactcactag
     1201 cgaaattttg ttcaaactca tctaaagcac gattccagtc gtatctacat ctttcgatta
     1261 gcaatccgta ttctaagctt tcaggagaaa aagaaagtgt tgtactcact ttactttgaa
     1321 gataaacagc atttttagct atcatctttt taattgactc atcttcttcg ttccaggtaa
     1381 cgtaaatatt atccttcaca gctattagca attcttcagt cacttgttca ggcgtcatct
     1441 aaccaccgcc ttaattgctt taacatatgc gtaagagcat tttttcttgt ttacaaatga
     1501 taaatcttca tcgtttgttg ttactccagc attgtgtaag atttttactt ctttaacttt
     1561 ttctattgga tcagtagcaa aacaaaagtc taattcctcg tgaactttag gaccaatatt
     1621 gaaatacatc atgttccaaa gctgtgccca catctcggct gtccagattt gtatatttgt
     1681 tttttgccct ctaaggtagc gatatagccg attagaatcc agataaacct ttttccaata
     1741 attcgcttta gggcggttaa taacccactg tgcgcctcct gaattagtgt ttatagtttc
     1801 caaagattct actgtaacat ttacaatgtt tgccatatct tttagaatat tttctccgtt
     1861 ttcacagctt ctaatataat caagacttag ataactacag cagtcgctac aataccaaac
     1921 atcatcttta gaaggcaatt tgcgcaaatt aattctttta ttgaaaatga catccgaatc
     1981 gatatagaaa tatcggtcgt cctcacgcga atgatcttct tctaaatatt tccaccataa
     2041 atatggttta atcgaaggaa tatactcttt gtcgtcccgc agatcatcgt acacatgaac
     2101 ttcaacacca tattccttct caaaaaaaat aggaatctga tcatcgtgtc tgctgaaaag
     2161 caatacgata tctttgattc ctagtttctt cagattagtt aaacaaactt caagctccca
     2221 tttaaaccga ttgattgccg gctgacaaag aatatacttc attctgatca cctacgcttg
     2281 tgttgtagtt gttgtggttg ttggttttgt agttgtagta gtagttccca aagcgctaat
     2341 atctaataca atgaaactat cgttacgttt aggttgaccg tttgcatatt gtttagctag
     2401 ataaatgcgt tcgtcttcaa caaaatggta ttcatctgaa gcttcaattt ttagtgtaga
     2461 tcctacaccc atgaagtaat ctgaggctac cccaataact gcttttcctt ctggcacagc
     2521 cgttgactgc aaatctgaaa ctggtactgg caatacttgt acgtattctc cattagcagt
     2581 tagtacagtc ttagctggga atactttaga ccagtaatca gttggattca caattaggac
     2641 cacatcagaa ggattcacat tacgataaat cggatcattc acaccttcga tattgaattt
     2701 tgatagtcgc gccatcaaac cgcccatagt tacagcatct aaagctgtaa taggttctgc
     2761 ttttttttca gcatattctc cgctagtttg tttgctcatg tcacgcatca ttccgactgg
     2821 catatcttta ccagtaccat caacaattgc ttgttctaat gcaattctca atgattctac
     2881 taaaacagta cggacataac gatctaacca tactggacct aaatcaagca ttgccttaca
     2941 tacaggaata taacctgata gcttgaactg cttcatgtta attacatcaa agccattatc
     3001 taaaactttt ttaacagctt cgcaaagttt accccaccat gctggattga ctccacgtga
     3061 cacaatccat tctgttacac cagttgtgtt aacaaaagta attttttgca atagtggatg
     3121 agattgttct aaatcttcaa atacacgttc aaatacagta gctggcacta attcttcgac
     3181 ccctgcaaaa ccttcgtttt tcactacttc gttatagaat tttgtttctt gtgtagttaa
     3241 tacacgctga ccacggttca ttaatactaa ttgatcttga ttttttgctg ttgcttcttc
     3301 taaaatttta tcctgaattt ccttagataa gcttaccata gctgcgctaa aagattcttc
     3361 gttaccatct ttaaaagctt tcatcaattg gtcgcttgca gctgttacac cttttaaatt
     3421 tttaactgtc attattttac atctccttgt ccaaatgttt tatttaatgc tgctgtaaat
     3481 gcagcaattt tttctgctct tttttcttta acgtcattca aaatttcttc aacgctttgt
     3541 tcttttttag cttcagtacc tgagctattt tctgcatcga taatttcatc gaccaatcca
     3601 taactcaaag ctgtttctgc atccataaac gattcttttt caagaagttc ttgcaatgct
     3661 tcatctgtgc cattgaatcg tgttttatat gaagccttta ccgatttatc aattgattcc
     3721 agttggtcag caatcgtacg gaagtcatcg acatttcctt ctccgtatgt ggaagcgcgg
     3781 tgaatcatca attgtgcatt gttgtagatt tttatagtat cgccagccat tgcgataatt
     3841 gaagcagcac tagcggctaa gccgttaatc acaacgttaa cttttgcttt atttgactta
     3901 agtaagttcc caatagcaat cccttgaaat acgtctccac cgtttgaatt aattactact
     3961 tcaatttctt cttgatcacc tagactatcc aaaatatttt tgattccctt gtcagtattc
     4021 ccttcaaaga accaactaga accaataaat ccctgaataa aaatttgcgg tactgcgcct
     4081 tcattcttta ctgctagaaa tgttttcatt gtcgtcattc gcctcacctc ctttcgatac
     4141 ttgttgattg tttttagtta taaatatttc atctgccatc gccttatcag agcgatcatt
     4201 tccaacgcgc tctcttcctt cgttgattgt aaatactcca tttctaatgc ctacatcaat
     4261 agcgtcaacc aaatctttga agctagtaat cttgatcata gttgtatcca cacgtacaaa
     4321 attccctgac aagtattctt ctacttcata gagactagcg ttaaacgcat cctgaataag
     4381 ttcagcaatc ggtatgattt cgaacattaa aaaagcgtcc acttgatccg ataacccact
     4441 catgtctccc tttagtaggt ttttcggaac gtgaaacgct gctgctgtca tctcaaagat
     4501 gtcgtctatt aagtttttta tatctcttga attgctttgg aagtttccgc tgaaatcttc
     4561 taatgtgtac tcattttgta attgaaatac cgcacctgca ttatcagctt ccataaaagc
     4621 cttaaattgt gatgtcatca ttttattgat ttgatcttgt gttgtattgt cttgcggtcg
     4681 gaataaattc cctttcagta cgtatctacg agcgttagag cgcttgtaaa cattcatggc
     4741 actagaaatg agtttcccat acgcttgata atacgcatcg actagttgcc taatttgttg
     4801 atctgcgtat tttatataga taacatcact ttctagaaat tctctatcaa ggactatgtt
     4861 gttaatttgc acttgagaaa acacatcatc tttcaatgca tattctgtga catcccaact
     4921 atccgcaata aatatttcgc tagaattatt agacggagaa acgatcaata cttcattgta
     4981 gaatattaat ctcctgatca gtttttttct aaattctgtt gcattatttt tcttattagg
     5041 agctacattc agcctatagt aaagatcatt ctttttattt tttccatctt catatgactt
     5101 gaattccgct ttactcatcg catttgcaat caaatcaata caagtttcaa tcgcaaattt
     5161 tcgatacaca aaatcaactt gcaatttaca aaagtattct tctaaaggaa ccgttgcttt
     5221 ttttgtgaag tatcctaccg ccttttgaaa aatccccact ttctcacctc ctttcaagtt
     5281 agaatactag aggagtaaat ccagttcctg tattttctac tgagctattt gtgactgtta
     5341 caggagcaga atcataaata tcatctaaaa aattcaaacc atgaaggaat gaaaaaaagc
     5401 catccgtttt tctagtttca ggttctattt tttcatagcg tatatttcca ttagaaatat
     5461 gctcttcata tacattcatg caataccaac gcataatcgc atcgtcacca aaaaataaac
     5521 gttgattaat aaaaaggtca tcaaccagat cttttaacat accatgtgta acagatccgc
     5581 ttcgaacaat ttccacagta aaacctgctt cttctaaagc gggcttcaat atttttgcac
     5641 ggtacatatc catagcgatt tttttaatat aatatttatt actcatttca agaaaccaac
     5701 ctacaatata atcagcttct atattttttc catgaacgat ctgtgatttt ccttgatcta
     5761 tagaaatatc tataacctct cgtttgatgt tttgtaatcg aagggctgat tcgtggataa
     5821 aagtatgttg tgtaaaataa acatctttat cgtattttcc tagcaaccca acgctggcaa
     5881 aatctcgtct atcagcaaaa tcgactgttc ctatcacttc atccattttt tcaggaaatt
     5941 ctttttcttt cgtatgcaga acatcatcat atgaagcaac agcaaatcgt gtatcttcca
     6001 taggtctgtt cattcgtttg gtcatgaacg taagtcttaa accagcatta cgttgcattt
     6061 gagagtattc ttgaaacatt ttccgtttta aatctgcatt gtaattaata gttggacaag
     6121 ctttttccca catgtcggga tcatcaactt cattatcgtt atccaagcga caaataaatg
     6181 gaaacaaact agaaaattct gctccatcct tgtcaattcc aagttctcca gaaagaatca
     6241 tttttgattc ttctataatg tcatcaagcg gaccaccacg aacatgacca ttagttgtat
     6301 cataaaattc tctataatct cgaattttac caccaccaga agtagccaca tttatcattg
     6361 aataatcttc attttcgtga atttcatcaa agcggtttgc acctggtcgc ttcccatctt
     6421 ttgttctagc atttgccgtg ttataacgaa gtttgctgtt tgtagcgata ttttgaataa
     6481 cttccttcgt agctttaaat acttttttat ctaaatcagg atgatcttta attactttaa
     6541 atacatcatc aaaactagtc tttgcttggc tttcattatt ggcatagata tcaatatcat
     6601 aatttttaat accgtgttta gcggttagta gaaagaagtt gttccaagaa gcaaaaccag
     6661 ttttaccatt accacgtccc attaatgaaa gatatctatt gaacactagt gttttatctt
     6721 ttttccatcg aacaccataa ataaaacatt gtagaaattt ttcccacgga attaattcga
     6781 atggaaagta ttgtgctggt atattgattg aatcctctac catctgctta tcgaagtaaa
     6841 tatcttctct agtaaagact ctttcttcta gataattttt tagcaataat tgctctttgc
     6901 ataccttgat agtgccttct tctatagctt tgaaccaatt ttcaatatgc ttataactca
     6961 ggaattgatt catttgcttc acctaccaat tcaggagtaa tggcaagttt atccaacatc
     7021 aatcccattt gtttgttgac agaaacaagc aacgctactg attcattctt tttaccattc
     7081 tccagtctaa tgccgttctc ggatatatct tcttccagtg atatcgccgt ttcccataaa
     7141 ctgatataac gatcaacatt atctaagaat ggctcaatat ttgttttctg actttccaat
     7201 tggcttatta aagagcggcg taatttttct ctgtagcgat tttgagacaa ttcgttttta
     7261 aacattttag ccctcctttc atgataaagt tcgaaaaaat ctcttttcct gacagccccc
     7321 tccgtttcat cacccccaaa aaatttgcga tttattttaa gggggggtta tctcaccatc
     7381 ggaatgaaag cttcagcgaa gtcaatgtaa taattaatct cttcaatgct atatccaaaa
     7441 acatttttta ttctttcaac gttattatct ttattcaacg cttctcttac ttgattcact
     7501 ttgtatttac tacaacagtt atctgataac agatccctaa tacctacata gcgaacgtat
     7561 atcaaacgtt taatcaatcc ttgtgtataa gatgaatact cttcaatctt ctctgtgtca
     7621 tactctctgc cattgtcatt gatgatcatg cacttaccac ctttcacttg catcgaagtt
     7681 agcgaagctt tctatcttct tctcttgttt atccaatgct gtaagatatc tgccatgaac
     7741 ttcattatga tgttcaacac ataaacaaat aagattatct aaatctaaag ctaagtcagg
     7801 tctatccttg acttccttta tatgatgaac gttctctact ctatgatact tacctagtct
     7861 tctacactct tggcattcat agtgatctcg tttcatcgct ttctccctaa gcctgcgcca
     7921 tttaggagac tgatagaact taaccaaacg atcttctctt atcaactgta atagccatct
     7981 atagaattcc tcagtcatga cccgtctcct ttcgcaatct tatttaatgc ttagctattc
     8041 ttttgccata caacggaata gcttcattgt tttcctttcg tttatatgta tcactcttta
     8101 ttggtcttct atactttcgt actatctcac cgttaccatt ttgcacagtg attacttcat
     8161 acttctgttc taagtattgt ggtctataca ttgttgttac ctcctttgtg caaaaataaa
     8221 aagaccactc aacgagtgat ctcatatgta atgcacaggc agggacgttt ccgatcctgt
     8281 gcttgagtca tttgacgatt catttgtacc aaatcccaaa actcaatcta acctaaccaa
     8341 ctatgtaata gcaacctaca ccgattccat cgattactat cgacctcgcc ttgctcgtgt
     8401 actttgagcg cccatttcca accctcagtt gctaatgaat actgaaacag aataccgtct
     8461 ctgttcatga ccaacgcgac agtacaaagc tttgtcgcaa gcaggttatc gtattcctaa
     8521 taaccccaac taatatttct ctcctaacct acacctgaga gtggcgcacg tctgcgctag
     8581 tattttatgc cttttaactg ctagtggcat cccgactagt cagccaacca atcggcatcc
     8641 gataagattg gttgtttccg taggttcctt aagtcactgg caatgaatcg aacattgcat
     8701 ggttgccgaa gcattgacct agcacgcatg cttagcgtct accctttccg ccacagtgac
     8761 actataaaat tattcttggc tgctactatt ttttattttg cccattttta aatccaatca
     8821 tatagacatt aagacagagc gcaaaaattg aaattattaa tggaatcatt tctcttcact
     8881 tacctttagt tatcgtgtga ataattaaaa aaacaataga cagcaacgga tgatagataa
     8941 taagaataat ttagaaggag ttgaaattca catccttatt cttaatattt ccgctgctgt
     9001 ctatcgaagc ttaattaaac gatgagggag atttcctccc ttacatttta ttttgtctca
     9061 gacctatcac taatctttcg acactatcat aatacaacat tgaataggta agtgattggt
     9121 ataaaaaggg tataaaatgg aaaccaaatg ggtaataaaa gggtataaaa aatgtaaaaa
     9181 ctggctactt gaaagcaacc agttctaacg atgaagcaaa ttgaatgata atcttgtttg
     9241 attctagctt gacagattct tcactcgtgt tatttctttg agcagttaca taaatgggca
     9301 gaccattgat ataacgatca tagaatatct tcttgcgcct ttcagtcaca tcaggcttat
     9361 gcggatgctg tatcgctgaa tagcctcgaa caaacaattt atgcagatac tcaaattctt
     9421 cttgtgcttc ttctttatcg atcagcattc tttctgcttc aaatatatga tcagctgtag
     9481 aagatggaac caaggaataa gatgctgtca cttttggttc tcgaggttga cctatcctac
     9541 atctagctga cagatatgct gaaagaaaaa caccaacatt atgttttgtg cgatccatat
     9601 ccacatcttt tgcctctggt gtctcatact tctttacatc gaaaagtacc atccattgat
     9661 tcctccattt atgatataat aattgtgtca gaattattaa ttaaggtcgg aggaatccgg
     9721 ctttttttat acccatgctt atgctaagct tttcaagtag cgaggttgca ctcgttaccc
     9781 atatacatgt tgagctatct ggcggaaaac agatggctca ctatttcaat attctgctaa
     9841 ggacagccag tggtcggctg tcttttttat attttaatga gaagccttac ttattttttt
     9901 atctttatta cgaaaaatga tattatctat tgataataaa gcgtatagga gatggttgag
     9961 tgactatgtg gagcatgctg ttattttgga ttcccgtttg tattggtatc gtcgcatttt
    10021 gttactttgt caaacactct agaacaaata agctcctcat gttatctttt ttacctatag
    10081 tattttttat tgtacaaatt gttaaatata cctatattga atcgcaagaa atattcattt
    10141 tttatgtggt aggtttattt atctctgtgg tctttttcat aatgatactt tcctattttt
    10201 ataaaaaata aatttttctc ttagaagtac attttttcgc tgtttatata gccttcatat
    10261 ccactaatcg aacaactgca atattcgctt tgctcttcgc tagctctttg tcacagtcca
    10321 tcgtattctc aatacgaata actgctgaat gattgtagac gtgttctaca tatccacgaa
    10381 atggatagat gaatccttct gcttcgcagc gaaccatgtc accgactttg aattttggtt
    10441 ccttacgtgt tttagggttc tttgtcggca tatctagcat taaaccgccg atgccgtggc
    10501 tactagcgta aaatccgtct tttagtttca ttatgcttcc tcctcaatcg tccaagcatt
    10561 tgtaaatttc tctaagctta ttagttcatc ttcatttaaa tatccgtaac catataagga
    10621 ttctaaaagg tcgtctttag aatatcctct aaagtcaagt ctgagaccgc ttaggatgta
    10681 tacaaaggtt ttatttccat aaaacttgat tgtgtatgct ttcatttcta ttcctcctgt
    10741 tctaatcccc attgagcgaa tgctgttaga acttcgaatt gctgtttttc tttcagtttg
    10801 ccccatgctt ttacataagg taacgagcta acgcctattt ctaatctttc atagacatct
    10861 tctgtgaaac tccacaacgt atattgaaga tcatttcttt taaattcatt ttttagaaaa
    10921 tcaaacacga tttgctggtt gtcgttaagt tgaattgatt tttctatttg tcttaaatct
    10981 tccgattcaa gtcgtagatg actgatttga gtcattttag cttgctcaac aattggaaac
    11041 cccaatccca aactatcttc taaattttgg agttcgcttt ctttttcaga tatgagttgt
    11101 tgcaaacttt tcatccttcc gccacctctt ccactggcac agcaaacggc cagtatcttt
    11161 catcaattgc tttgatttct ggctctgtta gctgataagc agatttttcc caccaacata
    11221 attcaaattt attaacaaaa caggaatcat ttctgttatt aaacttcttg acaagaaata
    11281 aattcccaat gataacttca tacaaaggct ctttctcgac ctcgtagcct acagcgtatg
    11341 cttgagcaaa taacagttgt cgttcatttt ctgttttgat tcgaggattc ccaaaaagcc
    11401 atgtataaac ttcgtcttct gaactgtcca tcatttcata taaagttgct cccccgcctc
    11461 tgtattcttc gatataatcc gccacaaact tcggcacaaa tggcttctgc ggttcgtcta
    11521 gttgtttgat tagttctaat gcgtgttcta aacccgcata ttttccatca tgaaaagtac
    11581 ttataccttt aaagtcttct atgctaccta caaggcttgc tagtttatca atcaattcct
    11641 gtttattcat cgctgtcctc catgtattcg tctaatatct ctttatattt ctctacaaat
    11701 ttgaaacgat cttgatgaag tttcttgctc caatttgttt gccgatccag ctcacgcatc
    11761 tgatcgaacc ctttttgaat ttcgttgtaa taaaattcaa tgtttgctgc tgctttccaa
    11821 tgcctgctac ttcgcactcc tgctcctgtt tcagccattt ctaacttaac taattcagct
    11881 cgttcttttg attttttgtc tttctgaatc ttcatcatga ttttcttgag gatgatgtca
    11941 ctgtattgtg taatgagatc cattatctct cctccacata cctaaactgt cgtccttttg
    12001 aatcaatcca taagctccta gctctatccc agataatgtt tttgctcaga ccagtaattt
    12061 cagataactg ttcagcagta cctgttacta gaattcggtc accatgccag attgcaatct
    12121 ttctcggcgt tctccgttta ggcttttcag tccacattga tttaccgagc ttttggactt
    12181 ctgcaactat ttctttgtct tcttgccaat tctcagaatg tgtcagttcg atgattcgtt
    12241 tcattgctgc tttcttatcc atccttaccc ctccaatcta cgaatttccc ttcttaaatt
    12301 ctctatgtgc aaatcgattg ccttcctcgc cgtttcattg accatcactg cctttgtccg
    12361 ttccagatcg tcaatttcac gttgaatgct tcgaattcgc atttgaatca cttcttctgt
    12421 tgtcatgatg attcctccac gtatctaaac gttctcttct tagcgtctgt gtagccacac
    12481 cttgctcttt tcctcacgat tttctcgtgc aagcctgtga gacttgctaa ctgttcagct
    12541 gtccctatca ctagaagttt gtcaccgtgc caaattgcga tttttcgtgg tcttggcttg
    12601 ttgctcttgt ctgcccacat cgctcttcca agcctcatca cttctgaagc agcttctttg
    12661 tcattttgcc aatcttctga ataagtcaat tcgataattc gctgcattgc cgctttctta
    12721 tccatcccga cattctcctt tcagtaattt gagtacttga tcaagtgcgc tctcacgtcc
    12781 gccgtggaac gtgttgagcc acttgtcttc gtacgataca ctttgtctta aagcttcttg
    12841 atgcattagt tcaatctgtg ctgtaaatgt ctttaggtcc atctgattac acctgctcaa
    12901 gttcattaag atgtttttgc aagcctttaa cacaatcaac aaatagtaat ttggtatatg
    12961 ctaaatttct taattgtgtt gtatcgatat aaagtgcgaa atagtatctg agcttactcc
    13021 aactcgaacg atcattctta attccttcga ttccagcttc ttcgagttga tcatatacgt
    13081 ctctcaggat ttctatttcc tcaccagttt tatactttgc tatttcatta attagttcta
    13141 gataatcgat tttcaatttc ccacctctta gaatggtgct tttgattgtc tattagctcg
    13201 ttctagcgct tttttcttga gataggcttc ttggtcgatt gcccactcag gaagcttctc
    13261 tcgtcttcct gtgcgcttgt atccactgct tgcgttctta gattcacttt tttcttttct
    13321 tgcccagctt cgaatagttg ccaaatagtt tttataagtc ttaccagatg attcacaata
    13381 ctcagatagc cgttctattc gctcttggta gtcattaggg aattctattt tgagtttctc
    13441 catctgctca tctgacaaaa gaacattttt atactctccg tatttatgac ggacgggctt
    13501 agccttcgat tttttcgaag gcgttacatt ctctatatct ttctctaact ctatatcttt
    13561 ctctaactct atctctaact ctggtgtagt tttgtccgga catttgtccg acatttgtcc
    13621 acttgttatt aaattctgtt ttgcttcctc aatttctttt ctgtattttc tcttcctatc
    13681 agcttcagtt gaagatttcc caataaaact ttgtatatca gacatataaa ttgctccgtt
    13741 atctaatacc tcaataagtt gtaaatcacg aaatatttgt actgcttttt ctacaactcc
    13801 cacagagtgt cttgtaatag tcgcaagcat tgtagaattg aatggaatcc tgtcattaaa
    13861 cattagtttg ccttcatgtt tcagacttct taaataaagt ttgagaagaa tattagaata
    13921 aatgtatcca tctggcatac tttctaaaag aaccatctcg tcgctatcga aaaagttctc
    13981 ttttagtttt aaatagtagt atcgtttgtt gtcagccaat attttttatc ctcctattct
    14041 aagtttctta attgtttcct ggtttaactt gatccctttg atttgatatt tatttttgaa
    14101 attgatcaca cctatcttgt gtttctc
//
LOCUS       DACDIG010000065        13929 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.065, whole genome shotgun sequence.
ACCESSION   DACDIG010000065 DACDIG010000000
VERSION     DACDIG010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.065"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            168..362
                     /locus_tag="IV247_002162"
     CDS             168..362
                     /locus_tag="IV247_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778221.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..930
                     /locus_tag="IV247_002163"
     CDS             631..930
                     /locus_tag="IV247_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778222.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1027..1461)
                     /locus_tag="IV247_002164"
     CDS             complement(1027..1461)
                     /locus_tag="IV247_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8778223.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1693..2403)
                     /locus_tag="IV247_002165"
     CDS             complement(1693..2403)
                     /locus_tag="IV247_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP8778224.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2393..3253)
                     /locus_tag="IV247_002166"
     CDS             complement(2393..3253)
                     /locus_tag="IV247_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAP8778225.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3258..3896)
                     /locus_tag="IV247_002167"
     CDS             complement(3258..3896)
                     /locus_tag="IV247_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAP8778226.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3911..4210)
                     /locus_tag="IV247_002168"
     CDS             complement(3911..4210)
                     /locus_tag="IV247_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8778227.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4253..5737)
                     /locus_tag="IV247_002169"
     CDS             complement(4253..5737)
                     /locus_tag="IV247_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP8778228.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5757..6218)
                     /locus_tag="IV247_002170"
     CDS             complement(5757..6218)
                     /locus_tag="IV247_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8778229.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="IV247_002171"
     CDS             complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="IV247_002171"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAP8778230.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7521..8291
                     /locus_tag="IV247_002172"
     CDS             7521..8291
                     /locus_tag="IV247_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8778231.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8564..9391)
                     /locus_tag="IV247_002173"
     CDS             complement(8564..9391)
                     /locus_tag="IV247_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8778232.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9959..11194
                     /locus_tag="IV247_002174"
     CDS             9959..11194
                     /locus_tag="IV247_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAP8778233.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12193..12453)
                     /locus_tag="IV247_002175"
     CDS             complement(12193..12453)
                     /locus_tag="IV247_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP8778234.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12480..13781)
                     /locus_tag="IV247_002176"
     CDS             complement(12480..13781)
                     /locus_tag="IV247_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8778235.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
BASE COUNT         4492 a         2839 c         2183 g         4415 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      901 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      961 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
     1021 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
     1081 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
     1141 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
     1201 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
     1261 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
     1321 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
     1381 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
     1441 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat
     1501 acagttcttt gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc
     1561 cttaaaattc ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata
     1621 aattttcttc acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt
     1681 accctagcat ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt
     1741 atctagtaaa tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc
     1801 catcagacaa atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc
     1861 ccatgtgaac ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt
     1921 tctttcctta aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac
     1981 ttcttctttc gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt
     2041 cgtgccataa gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg
     2101 agtatgagta atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag
     2161 atctgaagat ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac
     2221 catttgatct gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc
     2281 tagctcacgg tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg
     2341 caatgccaga tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga
     2401 catcatagcc agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc
     2461 ttcgaaagtc ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta
     2521 gacgtttcaa atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat
     2581 cacgaaactg gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag
     2641 tgatataatc gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca
     2701 aattgcctag gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta
     2761 aaaattttga aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc
     2821 catatttaga agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat
     2881 tcattgattt ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta
     2941 gattatgcgc caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg
     3001 gatcagcaga accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta
     3061 caccggagct gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc
     3121 gtgctaaccg ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt
     3181 ttacaaggga aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc
     3241 caatttgagc catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg
     3301 gccttcatcg gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg
     3361 atcccttcaa ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag
     3421 cctagttcaa tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat
     3481 aacagcgcat cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc
     3541 atggcttgtt caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt
     3601 tgtgctgcct ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct
     3661 gcttttgcaa cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact
     3721 agttttttat ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag
     3781 caaagaattg tgcccacttc aataatatct actacatcac caactttcat aacatctcct
     3841 aatgcatgtt ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta
     3901 gttccttcct ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt
     3961 ctttgactga catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag
     4021 taaacatatt caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt
     4081 ttccctctga aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg
     4141 ctttttccac acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta
     4201 aaattctcat cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc
     4261 tctgcttttc tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt
     4321 tccgatattg taactgcgga atagccaata aaccaatgac aactacggca attccagcat
     4381 aacctaggac tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc
     4441 cgatataacc accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta
     4501 cttgtatgat tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat
     4561 tggcataaac tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga
     4621 tcgttggtga tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa
     4681 acaagaaccc aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa
     4741 ttcctggaac agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt
     4801 catcgacgaa tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag
     4861 aagtcgtcat aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca
     4921 aataatcttt gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag
     4981 tagcaaccat attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt
     5041 ttttgtcttg cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta
     5101 attttgcaaa tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt
     5161 ctgtcaattc ctgcgtgatt cctactgtta agttagaagc tactgtccaa taacaaccta
     5221 aaatcagtcc cataaaaatc aatgcttgta tcctacctag atcaggaaag caaaacagta
     5281 aaatccaaaa agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac
     5341 gcagtttcgt atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta
     5401 ataaaatcat cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag
     5461 cattttgtcc aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc
     5521 caaccaagat tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt
     5581 aaccgaccgt tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa
     5641 tatagcctaa taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg
     5701 caaaatacgt ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa
     5761 ttatattctg ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca
     5821 accaatccat ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct
     5881 ggatcttttg ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca
     5941 ctttcaaaac ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca
     6001 tcactttttt ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg
     6061 tatttttcta cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg
     6121 atcaatacaa gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg
     6181 taatgaatca attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata
     6241 aaaacgacgg aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa
     6301 aattaaactc aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga
     6361 tataaggttt aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct
     6421 cttttggatc agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg
     6481 cattcaatga ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc
     6541 gtgggttttc accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg
     6601 caaataaatt ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga
     6661 ataaatagtt tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt
     6721 ctggatcttc acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga
     6781 tgtctttgat tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc
     6841 cccatttttg ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat
     6901 tttgatgaac tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga
     6961 ttaaagcgtc tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat
     7021 taggctgcat attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc
     7081 cattcccatg actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt
     7141 catgactttt cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat
     7201 ccactacttc tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta
     7261 ctaaccactt ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat
     7321 atcgttgttc caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt
     7381 atatgaccgt ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa
     7441 taaagacaga tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg
     7501 tccctcagga ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag
     7561 aaattttgga actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc
     7621 ttgatgtatc tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga
     7681 tcatacgcaa ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat
     7741 ctggtcctga aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt
     7801 atgatacgct gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat
     7861 ctaaccattt gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata
     7921 attgtagtta tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg
     7981 acatcgcatt aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg
     8041 gagtcgatgt agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa
     8101 tgatggtcaa caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac
     8161 tgacatcaaa gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat
     8221 tttgttctga aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag
     8281 attcatttta agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca
     8341 aatttatcga taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgta
     8401 tccatccaat cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta
     8461 aataggaatc aaacaatatc gaaaaaagga ggtatgacaa aagtcacacc tccttttttc
     8521 gaataaacgg tattcaaatc agaacactga tttcacaacc actttatttc aggtcgtttt
     8581 caatcacctt taccgcgtaa tcatttccgt tgaaacgcgc tatttttttt gcgtccatat
     8641 atctttctaa ataatcatct tctcctaatt gcttatggca agaagcaatt tcataaagca
     8701 agtattccaa tgcataagtc gatttatgtt tcttattata ctcaataccc ttctcacata
     8761 attggattgc tttatcataa tcctcgattt tacggtaata ggtggcgata tcataatata
     8821 caatgcagac catcagatta tcaaaatcag tctcactcaa tattttaagt gtacgctcca
     8881 cataaacttt cgcaaaatct aattctcctt ttgacaagta gatcattgcc acgtttgcag
     8941 ctgcttgtaa gtgatggata tctgtttcgg tcgtttgttc caacacttga ttaaaataaa
     9001 ataatgctgc atctggtttt ttcgcataaa gatgatcaag cagtccttga taaaataaaa
     9061 tttgatttaa aagatcagaa ttacttactt tttcctgatc gatcgtttgt aaaattttat
     9121 aagattcttg gtattttgat acagaattca atgattgaat ctcatttatt tttttcctga
     9181 ttttgtcttc ttctgtttct actaatatat cggagatctc gacattcagc tttttgcaaa
     9241 gcgcacttaa gatattgatg cctggcgaca tattcttgtt ttcgatattg ctgacagttg
     9301 cttgttcaca aatgccttct gctaattgta cttgtgttaa cccctttttc tttcggtatt
     9361 cttttagtaa tgctccatct actttaatca aaacaatttc cttcccttta aaaaattatt
     9421 tctcctttat atcatacaca aaaaaaagtt ctaaacaacc aaaaatgttt agaacagaat
     9481 aaaaatttta attttaaaat aatattcaaa ttaaacaact ggcgtatctg ctccttgatt
     9541 tctttttgtg ttatctgaat aattatcgtt tacagttata acacatagtg aaaattgtca
     9601 tggcgagaaa gtctaagaat atttttaaac ataactattt ctttcttacg tgtgtataat
     9661 gacgacagca cacctatcaa ttaatataat tattttattt ttttatgttc tcatgtttat
     9721 ttttaatgac acaacttctc gtatttatct ggcacaacat ataaatcgat caaaaaattg
     9781 ttgcgtgttt ttttgaaaat tggagtttca acttgattac aaaatttaaa tttagaagtt
     9841 gtgaaaacag cgagagacag tttaatattc aaaatataca taggctactc gaacgctgtg
     9901 ttttttagta tactagtaag aaacgaaggg atagactcat tccaataagg aggatttcat
     9961 gaagacaatt ctttttgctc ctgcagtatt caatttagct gagacaacac gaatgattga
    10021 ggttgccaaa gctatgaaag accgttatta ctgtgtattc tttggctttt ctaaaacttt
    10081 tgctcctttg atcacagaaa gtggctttga gtatcattta ctagcgccga tccttacgaa
    10141 aaaacaagaa gaacaaatca taaaatttga ccagttgcgt acacttaaaa acccttttac
    10201 caaagaaatg gttgcctctc gtatcactag tgaacagagt ctgattgcta agtttgagcc
    10261 tcgactgatt gtgacaggtt cgaatgtgac aattttttta tcggcccgca ataaaaagat
    10321 tcctttagta tatgttaaac cctatgcttt gagtgaacct ttttttcaat cagagcatgc
    10381 gctgatgccc cacgagttac gtttcttcgg ccctttaagc aatccccttt ggcactggac
    10441 aaaaaagcgt atagtaaata cccgatggat ccccaatgcc ttcaaacacg ctgcacaaaa
    10501 agaaatgctt cctttaccaa aaacaagttt acaaatgatg gatgctgatc taaatctcat
    10561 cacaacgcct aaactatttt tggaagaaga acagctgccg gaaaattatc gaatcgttgg
    10621 tccaattttt gctaagctca gtacttcctt gcctgaagaa gtgctaactt ttatacagcg
    10681 aaagcaacaa gaaagaaaac gtattatcta tttcgcaatg ggtagttcag gcaatccgcg
    10741 tatgttacgc aaaattttgt tcttcttaca aaaacaaccg gatctagcgg ttattgcacc
    10801 tattgactat ctgcttgatg acattcaatc tgactcagaa gatcttttaa taacagaata
    10861 tctgtctagt catcttttgc atgaattcat tgatttttct tttatccacg gcggggaagg
    10921 gaccgtccaa actgcctgtg aatccggcaa accgtttatc ggtgtgggga tgcaatttga
    10981 gcaaagctgt aatattgact attgtgtaaa atacggaaac gcaatcgccc tcacaaaacc
    11041 agtgactgaa agaaaactgc agattgctct gcgcgaagtt tcttcaccca agattcgtca
    11101 gcaggcacgc acgctgcaag ccgtttttcc aataaatggt ccacaacaag caattcaaga
    11161 aatcgaaacc ttcctgagca accacccaat atagtttcaa gtagaaaaag cttttttaat
    11221 agagactcat cagtaatttc gctattcgag acccagacag tgaaaataca aaaaaagtaa
    11281 atagtggatt gaaaagttag agttacttaa attacggtgt ctaaaaaaca caaaatcatc
    11341 cttaaaataa aagatctgac gctatccaaa tgagcatagg tagcgtctgc catttattct
    11401 tagtcaaatt ctttgacacc tttctctggt tggatcatct caacgtttct tcaaaagaaa
    11461 gaccataata attaggcaaa tacattttct taaaatgtgt catatagatc tagaagcatc
    11521 gctttgtaga gatttgtgcc gctagtcccc tcaacaatta ctaaatcaaa tgtaggattt
    11581 accactttat ctttgataat caacctccct cttctgttga gacaatgatc tcgcaatccg
    11641 atgcatactc ctatataaac tgccagcaat ccccacatat tgagtagatc atgtcagaat
    11701 gttctatgtc gtcagcaacg atacactaaa agtcacgttc accatgactg attgctattc
    11761 ccagcacaat tatttctata cacgtttcat gatttgaata aacactcaca tcatacatat
    11821 taccattcat acaccatact gcagccccta ctatatggtt gtaagctccc aaatctatat
    11881 ttgacttgat cactttggta gtagatctac taattctaaa tccattgctg ataaaatttc
    11941 cattttttcc tccttctttc tgccaaaaca gttaaaacaa taataatctt tatgtaaaaa
    12001 agggctgtga caaaagcctt gtcacagtcc catattccga ataaacggtg gtcaaaagct
    12061 tgcatctgcg gtaataagtt caaccaaacg aaaaataagc caacatttca caaaaattta
    12121 agaagcaatt ttcgtaaatt tcttcttatt tcttgaagct aaccacttct gttacaacca
    12181 cttttagaac atttaaagct tcgatgtgac aaaccatcta tgctgctcga tcataaaatt
    12241 gatagcaaaa attccacaaa gtacatatgt cccccatgaa aacaaaacat tcatatgcgg
    12301 tacaatgatc tttggaagaa tcacgatcaa aaatgatgcc agtgcccaaa tccatcgatt
    12361 gatttgaata tgatatgtag ctagcatatt caatataagg tgactagccg ttaataaaaa
    12421 gataaaaaca aaaaataaaa tcagccaata cattgtttcc caccttctaa aaaccaaact
    12481 tagacagtct ggacttcgtt ttctccacga ctttgttcat ctgacagcat ctttttatca
    12541 taagctttta caaatggata atagataata aatgctatca atgtgttgat catgatcaag
    12601 ataacaacac gccagtcatt tcctgctgaa agataaccgc cgattggagc tggtaatgtc
    12661 catgttggca aaacagtaaa tctattgacc atttctaatt tgactgcaaa ataactgata
    12721 atagtcaaaa ctaaaggaac taagttgaac gggatgaaca tgtaaggatt caacatcata
    12781 ggagctccaa aaattatggg ctcattgata ttgaagatac caggaataat agaaaaacga
    12841 ccaacctctt tcaagaattt tgatttacag aagaatataa acaaaacaga taccacgatc
    12901 gttgctcctg caccaccaat tgttaccgtc cattgataaa actgttcagg agcgatatag
    12961 ggtaattggg tggcagctgt accatctgcc aatgcctttg cattctcatc caacatgatc
    13021 agccacattg gacgaacaac agcccctaca atactcatgc catggatacc aaatgaccac
    13081 aacaaatgga tcaaaaacat tggtagtaaa acaccaagca aattattccc taaaatattg
    13141 gttaaaggct cgaataacga taataaggca gcgtttacat caaagcccgc gacgcttcga
    13201 atcagccaag taattgttaa aatgactgca cctggaatca atgcctcaaa actacgggca
    13261 acagcaggtg gcacttgttc tggcattttg atagtaatat ttcgattttt aaagaaacga
    13321 tatacttcca ctgcaaaaat catagataag atccccgcaa acatcccaga tgctcccaaa
    13381 gaactcatag gtaagaccca gccaagaggt gcctcttcag gagttaaaac gtttacagga
    13441 acttgtaaca taaagaacgt tcctaaagat aatataccac ctgtcacact atctaatcca
    13501 tatgatttag ctagatccga acccataaca aaagttgcat aaatagccat caatcccatc
    13561 gtcatcctaa agggtaaaac aatcgttgcc gaataagggg caataaattc attccatcca
    13621 ggaaacggta aattgataaa gaccataaaa aatgtcccaa tcaaaatcaa tgacaatgtt
    13681 gaaacaaccc cattacgaat tgctaataaa tgacgctgtg taccaatttt tgccattggt
    13741 gggacaagtt tctcttcgac ccatttcatc agcttttcca ctaaaaccac tccttttcaa
    13801 taaaatatga accattcatt gcatgacaac gcaaagaatg ttcataacta attatataat
    13861 taagaaattt tatttcaata tattctctta aataatcaaa tttattttta agaatcctga
    13921 aaataagtt
//
LOCUS       DACDIG010000066        13724 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.066, whole genome shotgun sequence.
ACCESSION   DACDIG010000066 DACDIG010000000
VERSION     DACDIG010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13724
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.066"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            504..1634
                     /locus_tag="IV247_002177"
     CDS             504..1634
                     /locus_tag="IV247_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP8778236.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            1726..2481
                     /locus_tag="IV247_002178"
     CDS             1726..2481
                     /locus_tag="IV247_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP8778237.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            2539..4578
                     /locus_tag="IV247_002179"
     CDS             2539..4578
                     /locus_tag="IV247_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAP8778238.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQVTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(4687..5838)
                     /locus_tag="IV247_002180"
     CDS             complement(4687..5838)
                     /locus_tag="IV247_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP8778239.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(5992..6522)
                     /locus_tag="IV247_002181"
     CDS             complement(5992..6522)
                     /locus_tag="IV247_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778240.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(6621..6971)
                     /locus_tag="IV247_002182"
     CDS             complement(6621..6971)
                     /locus_tag="IV247_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAP8778241.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(7128..8657)
                     /gene="ahpF"
                     /locus_tag="IV247_002183"
     CDS             complement(7128..8657)
                     /gene="ahpF"
                     /locus_tag="IV247_002183"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAP8778242.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(8667..9230)
                     /gene="ahpC"
                     /locus_tag="IV247_002184"
     CDS             complement(8667..9230)
                     /gene="ahpC"
                     /locus_tag="IV247_002184"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAP8778243.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(9374..10036)
                     /locus_tag="IV247_002185"
                     /pseudo
     CDS             complement(9374..10036)
                     /locus_tag="IV247_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            10220..11866
                     /locus_tag="IV247_002186"
     CDS             10220..11866
                     /locus_tag="IV247_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP8778244.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            11857..12939
                     /gene="uxuA"
                     /locus_tag="IV247_002187"
     CDS             11857..12939
                     /gene="uxuA"
                     /locus_tag="IV247_002187"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP8778245.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(13047..>13724)
                     /locus_tag="IV247_002188"
     CDS             complement(13047..>13724)
                     /locus_tag="IV247_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8778246.1"
                     /translation="YKLLKKANVPHVLYSREMVESLCTVFDLFIPEKEKILYSLAFAR
                     NKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILEKE
                     NVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQNDFM
                     ENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQSK
                     KENQVTS"
BASE COUNT         4511 a         2508 c         2605 g         4100 t
ORIGIN      
        1 cacataaaac taaaaaatta tcaaagaatc ctggtgcaac tgaaagaatt tgcaacggga
       61 ttctttcaac ttatttgtta tttttttcta aaaaatatta aagattgtta aatttcacta
      121 tttcatttca aattccattg cctaatcatt aggaaaagtc tatcatgaac tcgtaaatag
      181 aatatagtaa taaccttgtt aggtgaggct cctatacaaa cataggctgt tgcccagaaa
      241 cgtcgaaagt cgccaatggg taaaacagaa actgttggat taaggctttt tccaatagct
      301 aagaatgttt tctttacgtt gtatagtgct aaaactcgga cgatgagatg atgctaggtc
      361 agaattcttg ttcgaaaagg tttctgacct tttctttata tttttgtgac tgacttattt
      421 gatgagacaa actcgctcct cacctgacaa gtggattgct gtgtgaggag gagatgcggt
      481 gaaaaaagat cgattggtgg attttgggtc cttatttgac tttgtccatg atcggtcttt
      541 tagaagtata cagtgccagt tcttatcgtt tattgcaagc cgatgaaaat acaaaaagtc
      601 tattattgcg tcaactaatt ttcatatttt tgagttggag cgtgatcttc ttagctcgtt
      661 cagtcaaact acactattta cttcacccta aaatagcagg atacggttta gccttatcga
      721 ttttcttttt agtattagta agaataggga tattcggtgt cactgtcaac ggcgcacaac
      781 gttggatctc tctgtttggc attcaattcc agccttctga actggcaaat ctttttttga
      841 ttttttattt aagctggttt tttcgtgacg gaaatagtag cccaaaagat ctaaaaaaac
      901 cattcctgat tacagtaggt ataacttttc tgattttatt tcagccaaag attgctggag
      961 cattgatgat cctttcgatt gcgtgggtca tattttgggc agcggcggtt ccatttaaaa
     1021 aagggatcta tctaatcgtt actttttctg cattgctgat tggagcagca ggcggggtat
     1081 tatatttagg aaataaaggt tggcttccac agatgtttaa tcatgcttat gaaaggatcg
     1141 cgactttaag agattctttt atcgatagtc atggtgcggg ctatcaaatg acccattcgt
     1201 tttatgcact atataatggt ggcatatttg ggcggggatt aggaaatagt atcacaaaaa
     1261 aagggtatct tccagagaca gaaacagatt ttattttttc gattattaca gaagagcttg
     1321 ggctaatcgg tgcattgtgt gtgcttttct tattgttctc attatgcatg agaatctttt
     1381 gtttaagcag tcgctgtaaa aatcagcaag ctggattgtt tcttttagga ttcggaactt
     1441 tgctattcgt tcagacgata atgaacgtgg gaagtatcgc ggggctgatg ccaatgactg
     1501 gtgtgccatt gccatttgtt agctacggag gaacaagtta tctgatcctt tctttaggaa
     1561 tcggaatcac gttgaatatt tcttcaaaga tacaggccga agagttggcg ctctatcgac
     1621 ctgaaaaaca ataaagagaa actgagaaac tctcagtttg aattatcata aagtacaatc
     1681 ttttattttt caagcgagag gttcttttag aaagaggaat tcgcaatgaa agtatctcaa
     1741 aaaatcatat tatctatttt gttactgctt acattagtag ttggctattt ttcgattgag
     1801 tttatccatg gattttcgtc tgcaaaacag agttcaactg tgaaaaaggt agagccgaaa
     1861 agtatcccta ccacactaaa tgtggctttg attggttcag atgctcgttc gaaagacgaa
     1921 aacggtcgct cggattcact tctggttgca caatacaacc agaaaacaca acaagcaaaa
     1981 ctgatctcta tcatgagaga ttcatatgtc gatattccag gttacggaat gaataagata
     2041 aatgcagctt actcttatgg gggagttgat ttattaaatc aaacattaaa ggaaaatttc
     2101 aaatttgaag ctccctatta tgcaagtatc acatttcaag attttatcga ttgcgttaat
     2161 gaactgtttc ctggtggagt caagatcgat gcagaaaaat ctctagattt agatggcgta
     2221 tatataaaca aaggagagca agtgatggat ggtaatactt tactccagta tgctcgattt
     2281 cgggaagaca aagaaggaga cttcgggagg atcagaaggc agcagcaagt gatcaaagct
     2341 atctctcagc agctgaaaga cgtaacttca atattcaagc tacccaaagc ggttgggaaa
     2401 ttacttggta gtatccaaac gaaccttcca gaaagtgtgc ttctggattg tggtatggat
     2461 ttcctcaaag acatatgtta gaataaacag gtataaatag tgaaataaag gaatgacaag
     2521 caagagaagg aggaaaaaat gaaaagaagt gacaagcacg gcaaaaatcg aacaggcgct
     2581 tatattgccg gcgcagtgat cttaatagca gctgcgggtg gcggttattt ttactaccag
     2641 cactatcaag aaacccaagc agtagaagct ggagaaaaga cagttgagca atttgtccaa
     2701 gctttgaaca agggagatta taacaaagct gcagaaatga catcaaaaaa ggcggcaaat
     2761 aaaagtgcat tatctgaaaa agagatctta gataaatacc agaatatata cggtgctgct
     2821 gatgtcaaag gacttcagat atcgaatcta aaagtagata aaaaagacga ttctacttac
     2881 agtttttcat ataaagcaaa gatgaatacc tcattaggtg aattgaaaga tctttcttat
     2941 aaaggaacat tagacagaaa tgatggtcaa accacgatca actggcaacc caacttggtt
     3001 tttccagaaa tggaaggaaa tgacaaagta agcctgacca cacaagaagc agcaagaggg
     3061 aatattatag atcgaaatgg tgaaccatta gcaacaacag gcaaactaaa acaattagga
     3121 gtcgttccaa gtaaacttgg agatggaggc gaaaaaacag ccaatatcaa agcgattgct
     3181 tcctccttcg acttaacaga agatgctatc aatcaggcga tttcacaaag ctgggtacaa
     3241 cccgattact ttgtcccatt gaaaatcatt gatggagcaa cgccagaact tccagctgga
     3301 gctaccattc aagaagtaga cggcagatat tatcctttgg gtgaagcagc tgctcagctg
     3361 attggttacg tgggagatat cacagcagaa gatattgata agaatccaga attaagcagt
     3421 aatggaaaaa tcggtcgctc aggtttggag atggcttttg ataaggatct tcgtgggaca
     3481 acaggtggga aattaagcat tacagatgca gacggtgtag agaaaaaagt tctgatcgaa
     3541 catgaagttc aaaatggcaa agatatcaaa ttgacaatcg atgcgaaggc acaaaaaaca
     3601 gcttttgaca gtctaggagg aaaagctgga tcaactgttg cgacaacgcc aaaaaccggt
     3661 gatcttcttg cgcttgctag ctctccaagc tatgatccaa acaaaatgac aaacgggatc
     3721 tcacaagaag attacaaagc ttatgaagaa aatcctgaac atccattcat cagccgattt
     3781 gcgacaggtt atgctcctgg atctacgttt aaaatgatta cagcagcaat cggtctcgac
     3841 aacggcacta tcgatccaaa tgaagtgttg acgatcaacg ggcttaaatg gcaaaaagac
     3901 agttcttggg gatcttatca agtaacgcgt gtcagtagtg atgtatcaca agtagactta
     3961 aaaactgctt tgatctattc cgataatata tatgcggcac aagaaacgtt gaaaatgggt
     4021 gagaaaaaat ttcgtacagg cttagataaa ttcatttttg gtgaagacct tgatttgcca
     4081 attagtatga atccagcaca aatttctaat gaagatagct ttaactcaga tatcttgcta
     4141 gctgatactg gatatggaca gggcgaactt ctaattactc ctatccagca agcagcaatg
     4201 tattctgttt ttaccaacaa tggcacactt gtctatccta aattgattgc agataaagag
     4261 acaaaagata agaagaatgt catcagcgaa acagcagtac aaacgatcgt gccagatctg
     4321 agagaagttg tgcaagatgt aaatggtaca gcacattctc tttctgcttt agggattccg
     4381 ttggcagcga aaactggtac agcggaaatc aaagaaaaac aggatgtaaa ggggcaagag
     4441 aacagtttct tgtttgcttt caatcttgat aaccaaggtt atatgatggt cagcatgttg
     4501 gaaaataaag aagatgatga ttcagcaacc aaacgagcat cggaactatt gcaatacctc
     4561 aaccaaaatt atcaataaaa aattgctttt taagtacagt aaaaggactg tggcgttatc
     4621 gtcacagtcc ttttttcaaa taaacggttt tcacgatgtc tctttcacaa cttctttttg
     4681 atttactcac acttcataaa ctttttttgt aaaatatttg agaacaagtg gagaaataag
     4741 tgtacttaat aaaatcacga taactaaagg tgaatagtac tgtggttcga tcaattgact
     4801 ttgctgtcca atttgtaaaa tgatcaaagc catttctcca cgcgaaatca taccagcacc
     4861 gaccatcaat gaactatgtt gagaaaaacc ggcaatctga gcaccaaaat aacctccaag
     4921 caatttggtc aaaatcgcta caagcgtcaa tatagaaata aagagtagtt gttcactaaa
     4981 gtcagaaaaa tcaacttcta atcctacact gacaaaaaat actggtataa atactgcata
     5041 tcctaaagcc tctacattgt ggtatacttc ttctttaacc tttgtctgac tgacagctat
     5101 cccagcaaaa aaagcaccaa tgacagagct aagcccaaca agatccgcta cgtaagacat
     5161 tcctaaacaa ataactagcg atacgatgat aatagaagaa tttgcgtaca ctttttctgc
     5221 aaatgaaagt aggaaaggcg caatccattt gactaataag aaaataaaaa caaaataaat
     5281 tagctgttca atcaaaacaa gcggtaaaga agcattgcta gaagcttccc ccgttagaaa
     5341 agataggctg aaacttagaa ctaaaacgac taaaatgtca tctaccacag aagcacccaa
     5401 aattgttgat ccttcttttg tattcactac gtttagttct tttaatactt ctactgaaat
     5461 actgacagaa gttgccgcaa gaataatccc aaagaaaatc gcttcatttc ccgaaacttg
     5521 gaatgctttg cctgtcaacc agccaaaaac aaccgggaag atgatcccaa gcaaagcaac
     5581 gaacatacca ggtcggaaat attttttcaa aagagataaa tcactttcca gaccagctaa
     5641 aaacattaat aagatcacac caatctccga aaagtcatgg actagaatat caggtttgac
     5701 ccaattcaat ccagcttggc ctagcaaaac accaacaagt aattgtccaa tcactgctgg
     5761 gatacctaat ctccgcgaga tatgtgagcc aatcgttgtt gaaatcaata tcaggcataa
     5821 aataccaata aattccattc tcatcattcc tttgtataaa atatagcccg aacttagcat
     5881 aacatacatt attgaaaaaa taggaaaata tactcacaaa aaaaccaaca atccaaaaga
     5941 tttttttgga ttgttggttt ttatacggtc tattttaata actaaaaatg attaacgtat
     6001 agtcattttt cttattctga tgaaattgat aatctcctgg atgcccatga aaatcaaaat
     6061 aaagccgaat atttggaata gaagcatcaa acttttgaat ggattgaaca ataagacgaa
     6121 tccgccaatc aacaacagaa tactactata aacactccaa cttttagctt ttgtattcat
     6181 gctgaagaac aactggaaca agctgttgat gataacagct aatccaagaa gaatcggcag
     6241 gatagaaacg attgcaggtg caaaagcata gacgatcaga gctaagatca ggaaaaaaag
     6301 accactgaac agaccgatac tccaagaacc tgttttcctt ttaaaacggt aagcctcaaa
     6361 aagattcaac aacccaagca aaacaagata ggcggtgatc agataaccga taaaatgaaa
     6421 aacagcagaa gggtttagca caatcgcaat cccagaaata atataaacag cagctcttaa
     6481 taaggcgtat ttttgaatat tttgtttaat cgtctcaaac atataagaaa acctcctttg
     6541 acttatttta acgtgaacta gtcattttac caaattaaat gttacaaaat gagcgaagga
     6601 gaagaatatc tctggaaaaa tcaacttctg aggatctttt ccattttttt gccttttgct
     6661 agctcatcga tcattttatc taaataacga atttctttca tcaagttatc ctcgatgtct
     6721 tctactcgta caccacaaac aacacctgta atcaaggaac gtgaaggatt ggggacaggg
     6781 gcttccgcaa aaaattgttc gaaagtcact tgtttttgta gttgatattc tagttctgtt
     6841 tgggaataac ccgtcagcca acaaatgatt tcatctactt cctcttttat acgatctttt
     6901 ctctccgctt ttgctacata cataggataa acgcttgcaa aacttgtcgt aaaaatacga
     6961 ttttgcgcca taatcagtca cctttctttt tttcaaatta tttactactt atagtatata
     7021 agaaaaagag aagaaaaagc gattattcaa aaaaacaaaa aggattgtga caaaattctt
     7081 gtcaccatcc ttttttccgc tctctgactt tttacaatct tcatagctca agtgcgcatc
     7141 agataatcga atgctcctaa agctgcagta gctcctgaac ccattgaaac aatgatttgt
     7201 ttgtatggac tatctgtaca atcaccagct gcgaaaacgc cggggatatt cgttgcgcca
     7261 tttttctcgg tcactatttc atttcttgga ttcatttcga tcgttccatc tagccaatcc
     7321 gtattaggga gtaagccgac tagaatgaat actcctgaaa cagctagtgt atgcatctcc
     7381 atcgtacggc gatcgatata ggagagagat tctacctgat tcgtaccgct gatttctttc
     7441 gtcgctgcat gtttgataac agtgacattc gcaagagaga ctagtttatc ctgtaagatt
     7501 tgatctgctt ttaattctgg taagaattct agaacatata catgttttgc taacccagcc
     7561 aaatcgattg ctgcttcaac accagaatta ccaccaccga taacagcaat ctcttttcct
     7621 ttaaataaag ggccgtcaca gtgtgggcaa taagcaattc ctttattttt aaactctttt
     7681 tcgcctggta cattgataga acgccagtgc gcacctgtcg cgatgatggc ggtttttgct
     7741 ttaagtgata taccgttagc taagccaata ttaataaaat gttgctcttt atttaaggaa
     7801 acggcctgtt gattcatcat aatgtcgata gggtaactac gcacatgact ttctacttgc
     7861 tgcatcaatt ttggaccttc tgtgtaagag gtaccaatca tattttcaat ccctagtgtt
     7921 tcaacgactt gtccgccgaa agaatcagcg acaagtcctg tacggattcc tttacgtgct
     7981 gcatagatag ctgcactact agctgctggt ccaccgccaa tcactaatac atcataaggt
     8041 tctttttcaa acaactcttc ttcaatcaaa gggccagtga ttttttcaac aatttgttcg
     8101 atcgtcatgc gtccgctact aaattcttct ccatttaaaa acacagttgg cactgccatg
     8161 atttttttat catcgatttc tttttggaac atcccacctt caatcatcgt atgagtaata
     8221 tttggattta atacagctaa tatattcaat gcctgtacaa cgtcggggca attatgacaa
     8281 gtcaagctaa cataactgtc aaaatgcaat gtttgtgtta tttttttgat tttaagactc
     8341 aaagattcac tgattttcgg tgcccgtcca ctaatttgta acatagccag taaaaaagag
     8401 gaaaattcat gaccgagggg gataccagca aaaacgattc cactttcttt gccaactgga
     8461 ttgatagaaa aactaggtgt gcgcatcgaa tctctttttt cccaagagat tttaggagac
     8521 atttctgcta tttccttcgt aaattcagct agtttttgac tattttcact attatccaaa
     8581 ctaacagaaa aaacaatagg agattcaatc agtgctaaat attgttcgag attttgttta
     8641 gtttctttgt ctaacatatc caccacctaa atctttccaa caagatcaaa acttggtttc
     8701 aatgtttcgc cactttcttt ccattttgcc ggacaaactt cacctgggtg ggtacgtaca
     8761 tattgagcag cgcgaatctt atcaatcagt gcactagcgt cacgaccgat tccatctgca
     8821 ttgatttcca tagcttgaac gattccgtct ggatcaataa taaatgttcc tcgctgagct
     8881 aatccttgct cttcatccaa aacatcaaaa atacgagaaa tctgatgaga cggatcaccg
     8941 atcatcacgt attcgatttt accgatagca tctgatgtat catgccatgc tttgtgagta
     9001 aagtgggtat cggtagagca agaaaagact tcaacaccta aagatttcaa tgtactgtat
     9061 tgttcttgta agtcttctaa ttcagtagga caaacaaaag taaaatcagc tggatagaag
     9121 caaagaatac tccagttccc cataagatct ttttcactga cttctaaaaa ttctccttga
     9181 tgataagcat gtgcattaaa ttcttcgatt tttttcccaa tcaatgacat agtaaaacct
     9241 ccaataataa ttatttattt ataattattg taatttagtt ggagttgcaa agtcaatgga
     9301 tattttgtta tttaataaag attactcaaa aacgaaggtt aattataaaa atagaagagg
     9361 tcgcaagaaa atgctactgc tggaaatact cgggataacg ttgagtaaca gtttcctgtt
     9421 ctttcagcat catgtgtaag tgcaatcccg tttgaaatcg agcttcttct ggttttttat
     9481 caactaaagc cttgagtaaa tcataatgtt gttccatgat cgtttcccat ggaagcccct
     9541 gaatggtcac tcgtaaccag cggaaacgtt ctaaatgggt attatggtca tctaaccagt
     9601 tccaaacttc ttttcttcct gcaatttcga aacaagcttc atgaaataaa ttatctgcac
     9661 ggaaaaaagc gcgttcgtca tttattttga ctgctttttc ttgttcttct aaaatcgttt
     9721 ctagcactat tcgtgtttgt gtgtccaatt tggaacaaca ttcaatcatg atttgctctt
     9781 ccaactgttc tcggacaaag cgagcatttt ctgcagaaac aagattaatt ttggaaacat
     9841 acgtaccact ttgaggtatc gtttacacga gttcttgctg acgtagttga atcaatgctt
     9901 cgcgaatggg agtccggccg atagcaagat gttcctccag tgccttttca gatattttcc
     9961 ttccaggttc taagtcagca tagataattt gtttacgaat cgcttgataa gcttgtgatt
    10021 gcaagctgct tttcatcttt attcctccaa aatcatttct atgaacgtag tataacagaa
    10081 gaggtctgaa aaacaatctg gtatgtcaca ttaactagta aatttgtata aatagtaaaa
    10141 ttttggttga catagcgatt tttattgcat aaaattcact tgaatacaaa aactaatatg
    10201 ttagtttagg aggggaaaaa tgattgattt aaacaactgg aggaacttta tccctgaatt
    10261 atctcaaaaa gaagtagaaa tacctcattt tgaccgtgca tcattaagag aaaatggaca
    10321 aaaaaatcct gtctggatcc atttcggtgg cggaaatctt tatcgaggat ttcatgcaga
    10381 aatcgctcaa cgtttagcgg atctaggcga attgtccact ggtgtcattg tttgtgaaac
    10441 attcgatgaa gaaataattg aaagggctta ccaagatttt gatgatttga ttcttgaagt
    10501 ggtcatgcca agcagtggag agttcgtcaa acgtatatta gctgcgacag ctgacagtct
    10561 gtattgtcat ccagattgtt ctgaacactt tgataagatg attcgacact ttgaaaatcc
    10621 tagtttacaa ttagttactt ttgctattac tgaaaaaggt tatcgattga aagatacaaa
    10681 taataaatat ataggaccga ttgcagtcga tataaaaaat ggtccaaaga agccatcaca
    10741 tacaatgagt attcttgctg tgctacttta taaacgtttt ttatctggag cttggccgat
    10801 tgcacttgtc tctacagata atttttcaca aaacggtagt caattacgta cttctttgct
    10861 aacgatcgca aaagaatggc aaaaggaagg ttatgtacaa aatacattta tccaatactt
    10921 atcgaatgaa gaaattgttg cgtttccatg gacgatgatt gatcgtatca ccccgaatcc
    10981 ttccttgtca gttagcaaaa tgttggcaaa agagggcttt tcaaatatgt cgattttcca
    11041 aaccgcaaaa ggaaataata ttgctccatt tgtcaataca gaggacatcc attatttaat
    11101 catggaagat cattttccca atggacgtcc gaaattcgaa aaagctgggg tattcttgac
    11161 tgatcgtgca acggtagata aagcagatac gatgaaggtg acgacttgcc ttaatccttt
    11221 gcatacgtct ttagctatta tgggttgttt actggggtat aggagcatag ctgaggagat
    11281 gaaagatcca gatttatcgc tgctagtttc tgaaatcggg tacaatgaag gcctcccagt
    11341 tgtggagtct ccaggtatca ttgatccaaa gatctttctg gaagaagtaa ttactgtgcg
    11401 cttttctaat ccaaatattc ctaatatgcc tcagcgaatc gccacggata cctctcaaaa
    11461 ggtggcgatt cgttatggtg agacgattaa aaaatatatg gaaatagaag ttaaagaccc
    11521 gcagacactt gtattcatac cattgactct cgctgcttgg cttcgatacc ttattggcat
    11581 tgatgatcaa ggaaaaccat tcatcccgag tccagaccca ctattgacag accttcagca
    11641 taagctttcc gatattcaac tgggtacgca agatacatca catattcatg agaaaatggc
    11701 tcctattttg tcaaataaag aaatttttgg tgtagatctt taccaagcag agcttggcag
    11761 aaaaatagaa gaaatgttca tcaagatggt agcaggacca aatgccgtac gaaaaacatt
    11821 acaggattac ttacaagaac atggaggaaa tttatcatgg aaatgacttt tagatggtac
    11881 ggatcacagg aagaaaagat taccttagat gaaatcagac agattcctgc ggtcaaaggg
    11941 attgtaggca cattgatgga tataccagta ggagaggtat ggcctaggga gaggatcaaa
    12001 gaactaaaag aagaaattga gtctcgtggg ttaacactaa aagtaattga aagcgtcaat
    12061 gtgcatgatg ccattaaaat tggattacct tcccgtgagc agtatatcga aaactataag
    12121 cagacaatcc gaaacttggc agaatatgga atagaagtca tctgctataa ttttatgcct
    12181 gtatttgact gggtgaaatc tgaccttgac taccgcttag aagacggctc atccacgtta
    12241 gcttttatat ctgatgatat tccagatgat ccaagagaaa tcgtcgcgcg catcgaacat
    12301 tccagcaatg aatttggatt gccgggatgg gaacctgagc gcttagctcc tatcaaatcc
    12361 ttgcttgaag cctatgcatc agtagacgaa gaaaaacttc gagaaaattt tgcctatttt
    12421 ctccaatcaa tcatcccaac atgtgaagag gtaggggtca aaatggctgt acatccagat
    12481 gatccgccat actcgatttt tggattagct cgaatcgtaa agaatcgaga agatttagat
    12541 tggttgtgtc gcatagtaga cagcccttat aacggaatca ctctttgtac agggagtatt
    12601 gcagaagatc cagaaaatga tgtatatggg attttagcag aattttgcca aagagatcga
    12661 atcccatttg cacatgttcg aaatattaaa tttttagcta atcgagattt ctatgaggca
    12721 ccacataaat ccgaatacgg ttcattagac atgtaccgca tactaaaaac aatgtatgac
    12781 aacggattta acggatacat ccgtcctgat catggaagaa tgatctggaa tgagacagga
    12841 agaccaggat acggcttata tgatcgagca ttaggtgctg tttatctaaa tggattatgg
    12901 gaagcaatcg aaaaaggaaa caaaagtaaa gataattagt gaaaaaaact gtaattttat
    12961 tatgtaaata agaaggatgt tcaaattctt gatacataaa tccggaggtt gttaataagc
    13021 attcggattt atgtatctta tttggtctag gaagtaactt gattttcttt tttcgattga
    13081 tccatgagtg cttcttcttt taattcaaag atatattctg agatcctcat aatcactttt
    13141 ttccagtctt ctggtgtgat gattcctttt aaaatataga tttttttgat atgagaaggc
    13201 aattgtttca aagggcagtc gctgataacg atgttgcagt tctccataaa atcattttgt
    13261 attttgatcg attcatttga aaaaatttgt tctatcttaa cgatcagatc tttactgata
    13321 taaaaatcac gcgtatagtt cacaccaatc acaattcgcg gctgtttttt gtagcgatca
    13381 tacaaatgta ctaggtcttc aaccatccat tgcacatttt ccttttctaa aataactggt
    13441 aacaagtgtt gattttgttc tctgaaatcc atataaaatt gttcaatggc ggtttggact
    13501 tctttcaata cgctatgttc ttgataaagc atgaaagtag gaaggggcgt atttggttga
    13561 ttgaagttaa gaaatccatt ataaagttta tttcgtgcga atgccaatga gtaaagaatc
    13621 ttttcttttt caggtataaa caaatcaaat acagtacaaa gggactccac catttctctt
    13681 gaataaagaa catgaggaac gtttgctttt tttaaaagtt tgta
//
LOCUS       DACDIG010000067        13535 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.067, whole genome shotgun sequence.
ACCESSION   DACDIG010000067 DACDIG010000000
VERSION     DACDIG010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13535
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.067"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            37..1635
                     /locus_tag="IV247_002189"
     CDS             37..1635
                     /locus_tag="IV247_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP8778247.1"
                     /translation="MLFLSFAYVIPLFMSKGIYHSVNQDTYFHLSRIIGLDNVWSSPV
                     NFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFITFLTMVVSYFSMK
                     QIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLILMGCYEIYIRDYQK
                     WYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLKATVLTFFLS
                     AGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYTIGLFLFLGLIGAF
                     IFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVWRLNGFSSLFIAYT
                     MSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLTLIAPEDAVAIAEN
                     YNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIELDNSNNKNTVLTTP
                     IYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTKLAIASRYLSIVTL
                     ILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(1780..2520)
                     /gene="truA"
                     /locus_tag="IV247_002190"
     CDS             complement(1780..2520)
                     /gene="truA"
                     /locus_tag="IV247_002190"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP8778248.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(2711..3346)
                     /locus_tag="IV247_002191"
     CDS             complement(2711..3346)
                     /locus_tag="IV247_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP8778249.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(3493..4734)
                     /gene="sstT"
                     /locus_tag="IV247_002192"
     CDS             complement(3493..4734)
                     /gene="sstT"
                     /locus_tag="IV247_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAP8778250.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            4932..5930
                     /locus_tag="IV247_002193"
     CDS             4932..5930
                     /locus_tag="IV247_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778251.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            5943..7961
                     /locus_tag="IV247_002194"
     CDS             5943..7961
                     /locus_tag="IV247_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAP8778252.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(8021..8737)
                     /locus_tag="IV247_002195"
     CDS             complement(8021..8737)
                     /locus_tag="IV247_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP8778253.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(8964..9506)
                     /locus_tag="IV247_002196"
     CDS             complement(8964..9506)
                     /locus_tag="IV247_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP8778254.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(9511..9738)
                     /locus_tag="IV247_002197"
     CDS             complement(9511..9738)
                     /locus_tag="IV247_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopZ"
                     /protein_id="HAP8778255.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(9817..11697)
                     /locus_tag="IV247_002198"
     CDS             complement(9817..11697)
                     /locus_tag="IV247_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP8778256.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(11886..12500)
                     /locus_tag="IV247_002199"
     CDS             complement(11886..12500)
                     /locus_tag="IV247_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8778257.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            12642..13202
                     /locus_tag="IV247_002200"
     CDS             12642..13202
                     /locus_tag="IV247_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8778258.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
BASE COUNT         4236 a         2634 c         2135 g         4530 t
ORIGIN      
        1 aaaaaaaaca aaaaccaaat aatagatatc atttttatgt tatttctaag ttttgcttac
       61 gtcatccctc tattcatgtc taagggaatc tatcatagtg tcaatcagga tacttacttt
      121 catttatctc gaatcatagg actagataat gtatggagtt ctccagtaaa cttcaataac
      181 ttcgatcatc atggaacaat gatgaatatt ttttatccat ggcttactct ttatccagct
      241 tttttatttt ataaaatgat gggaaatctt gttttaagtt ataatatcta ctattttttt
      301 atcacttttt taacaatggt cgtttcttat ttttctatga aacaaataaa aaacaatcgt
      361 tatatttcgc tgttattttc gattatttat acattttccg cctatcgtgc cattgacata
      421 ttccatcgtg cttcattggg agaagctgtc gctttaacat ttttgccttt gatactaatg
      481 gggtgttacg aaatttatat tagagattac caaaaatggt actggttgtc tattggcatg
      541 acgctagtag tgtatactca tttactttct gtcgctatgg tttctgtatt tattggagga
      601 acgttgtttc tttcttttta tttttgggac caaaaaatcg ctagactctt aagtctttta
      661 aaagctaccg tcctcacctt ttttttaagt gcgggattct taattccttt tattcaacaa
      721 tctagagcac aagaattgaa agtcccactg ggaaaagaat taagtggtat ggctcctagt
      781 gatatgctga cacatatttt aaataataac tataacaact atactattgg attattctta
      841 tttctcggat tgattggtgc ttttattttt ataaaaaaat taactgctga tgatttattt
      901 attttctttc ttggtgtatt tgttcttttt tgttcaacca atttatttcc ttggcaactt
      961 tttaaccata caccagttaa gagtctacaa tttgtctggc ggttaaatgg tttttcttct
     1021 ttatttatag cctatacgat gagtatagtg atctattata ttttttctac taaaaataat
     1081 tcatggaaaa taatggtctt tacgtttctt gcactaatat tgcatctctc aggagtaagc
     1141 aattctatcc atgctaacaa agattcatta acacttatag cacctgaaga cgctgttgct
     1201 atagctgaaa attacaatca tactgattat gcaaacaaag agtcaatcta tcatcctgat
     1261 atgattaata atgatcaata tttactaggt aaccaggaaa taaatccaac aactcatttt
     1321 atgagtaaca aacttacaat tgaattagat aattcgaata ataaaaatac tgtcttgacg
     1381 acacctattt atcgttataa agggcaagtg gcttctatta atggcaaact agtacaaaca
     1441 aaattatcta agtttggcac aactgaatta actattcctc caggtataaa taaagtcgtt
     1501 attacttatc agtacacaaa actagcaatt gcttcaagat atttatctat cgttactttg
     1561 attttatttt tactttatcg atttactttt tctaaacaaa aacaaccgag aagagaagta
     1621 caacattctc actagatttt tatttaagaa agacttttta gctgtacaca taaacaggct
     1681 aaaaagtctt tttttatatt tctttaattt gcttcataca ttatttaaca tcgatattta
     1741 tactgtgaaa catgcgtttc atccattatc aaagtatttt tatcgataat aaacttcatc
     1801 caaaaacaga ccttgtgcag ggacggtttc tcctgcatgt tgccgcactt tgctttcaaa
     1861 tatttcatcg attgtttcta tcggcatggc acctgtgccg atttctagta atgtccccat
     1921 gataatccgc accattttat ataagaatcc gtctccgaca aaagtaaaat gaagcatttc
     1981 tccctttcgt tcgatagata attcatcaat cgtacgcact gtggattttt ttgatttttt
     2041 caatgcagaa aaaccaatga aatcatgtgt acccactaat ttttcgcaag ctgcattcag
     2101 cttgtccata tccaactctt gagggaaata aaagctgtgg tttcgctcaa atgctgaagg
     2161 aatcacgttg ttccaaacat aatagctgta ttttttgccc accgcattat atcttgaatg
     2221 gaagcgctca ggcatttctt cgattctttt gatgacaata tcccgcggaa ggtaacgatt
     2281 cataaagtcg atcatttctg ccagtccaat ttctgtgttt gttttaaaat ttgctacttg
     2341 tcctcgcgca tgggtgccag catctgttct tcctgatcca ataatttcta tctcttcatt
     2401 tgtcatctga tgcaatactt tttcgatttt tccttgaatg gttttatcgt catctccaag
     2461 tctttgccat cctgaatatc tttttccgtc atattcgatt gtcaacttga tgtttctcat
     2521 attttagtgg ctcctctgtc ttccatttgg tcttctctct acctttttaa gattgtagca
     2581 caaaatatag ctccttgctt ctctaagaca gaagctatac aagaaaactg tggaaatata
     2641 tatttcagaa aaacaaaaaa cagccaccgg atgaagtatc cgctgactgt tcaatttgac
     2701 ggctcaagtt ttactgtttg agatgcaaga tactcaacca ggcaatgggc acataattaa
     2761 agaaaaaact gattctttgg caaaaaccta ttttattcag taatggccaa gaatttatag
     2821 aaggaattct tgccagtcca taaatcgccg caaaaagcaa actgatcaca gacagtccaa
     2881 ggtataagtg gcttacgtcc atttgcccat tttttcgata gatcaagaaa agaagaactg
     2941 gaaacaataa aaaaccactg taacctaaac ctgatccgat attatggacc catgtcgaga
     3001 cattccacga agattcatgg gtattgatac tgaacaaacc tgtaaagata caatcaccta
     3061 taccatagaa cgcgatggct gctgtaacta gggtagctaa cacacgagat gtactaacaa
     3121 atgcagcata caatgcaggc aatgacagga tgaaaaagat ccctgaaacc acagaccaaa
     3181 taagaaacgc ttttcttaca ggactggaaa cctctccaaa tacactgatc acgctcttca
     3241 tttgatctaa ttcaggataa tacaatccta atacgtacgg agtcaaaaaa tcactcagca
     3301 cgcctaataa taaaaagaaa aaaccaaatt tacgtaaaaa atccatgata cgcctccaat
     3361 aacaattacc tatgctttta attgttatta taaacgactt tatcttgatt taacagaaac
     3421 ctctatttac acgaacatct atataactca ttaaaaactc gaatcatttt aaaaatacta
     3481 aatatctaac gttcaacgtt cacttaatcc atctaaacaa tctttcaatt tgattttttc
     3541 cccacttctt ttacgcgcgc ctagctctcc tattgcagta aacaacagat cacttgacga
     3601 atttaaggca gtttccattg aatcctggat aacaccgata ataaatccaa taccgacgat
     3661 ttgcatcgcg acatcattag agataccaaa caaactacat gctaatggaa tcaataacag
     3721 tgaaccgccc gcaacacctg aagctccaca agcagctaaa gcagacaaaa tacataaaag
     3781 gactttcaaa acaaaaggtg cttcaatccc taaagaatgt acagcagtca acgtcatcaa
     3841 ggaaatggta atcgcggcac cgcccatatt gatcgtagca cctaacggga tcgatatgga
     3901 ataagactct tttgtcaatt tcaatctttc agccaacatc atgtttatcg ggatatttgc
     3961 ggcagaactt cgggtaaaga aagcgggaag tgcgctttct ttcaaacaaa agaaaatcaa
     4021 tggataggga ttttctttaa taaacaaaaa cgtgaggaat gggtacacaa ctaatgcgac
     4081 aaacagcatc gtaccgatca aaacaagtaa aagctgcaga tactctgcca atccagcgat
     4141 tccagtcgta gcaattgatt gataaaccaa tcctaaaatc cctatcggag caaatgcaat
     4201 aatcacttgt acgacttgtg acaacgctat agatatctga tccaccaact cttttgtcga
     4261 ttctgaacgt gctcgaaaag ctagtcccaa caatacagcc cagaataaga cgccaaggta
     4321 attcgcatca ataagagatt gaacaggatt ttgtgtgaca ttcgtcaaca tagtagataa
     4381 aacactttcc aaactttgcg gtgctttttc agatactgtt tcttgtaaag taataggaac
     4441 agtaaaaatt gaacttacaa taactgctac aagtgcagaa agcaatgttc ccactaaata
     4501 taagatcaaa ataggcttca cgaatgtctt attcccaatt ttatgcttgg caattgacgc
     4561 catcgtcaag aaaaaaacca gaataggtgc gattgccttc aagcaactga caaacaacgt
     4621 tcccaatatt gaaataaatg tccatgtggg gaaaaagata ccaaacaata ttccgactaa
     4681 aataccaaca acaatacgtt gaactaacga catatccaaa aacttctcta acaaatgacc
     4741 accctttcac aattttaaca atcattgtat cattttatgc aaagaaacta aaggtataat
     4801 tttctaaaaa ttctaacaat acaaacaacc atttcttgct aattttgtac tttttctgtc
     4861 tacacgttgt tttttgagat attctaagtt aggatgttat taatgggcaa cttatcagga
     4921 aacgttttct tatgtttttg tccaattttt ttgtgaggtg tttattcttg aaaaaaataa
     4981 agaaggagag ttatggcttc ctgacaatct ttgtcgcttg tataatgatg gttttacagc
     5041 gtatattgat ttcagcagtc actattccta catatgcaac aaaccctggt cgattctaca
     5101 tctatacatt tttacaaact tccgttgtta ttggtgcaaa tttgatccca ctttacatgg
     5161 gttaccaata tcaaaaaatc gcccgtttaa aagttttaca ttatttaagt cgttttgcct
     5221 ttatattcct aatagccaca ctagtttgca atatcttttt ttacgtacaa gctggcagct
     5281 tgaatattcg tgattattgg cttatcctaa cacctatcag tcagaattac tttacttatg
     5341 ctgtttcttg tactttgctt ttttgtagta ttccttatac agttggattt ttaaataaat
     5401 tatcgaaaac aacaattaaa tatctactat tatttttaac aattggatta gttggctgct
     5461 ctactctatt taataaagac ccgtttgcat tacagaatgg acagagtatt ttatggattt
     5521 tttatttatt tttaatgggt tatggtttga aagaatggaa ctggaaagaa aaggtccgct
     5581 ataagcttcc tcaagtattt atcagcttga ttgttttatt cggtttgatt attttgatga
     5641 cacaaatttc actcatcatc agaggaaata cagatacagc gctgaggttt tctaaaccat
     5701 attctctctt cgcaatgtat tatactttct cttcgttttt atttttagaa atgctatctg
     5761 ataaattagg tataaaagga agaggaaatt ttcttgctgt atttttgatt gtcactcaag
     5821 ttatatgtaa tttcccttta gtttcttatc gcgtatctac atttatgaaa cgtgaatttc
     5881 caaactcagg gaaggcatgg ctgataaata tcgggcagtt ttttatttga tatttattag
     5941 cagtgtccct cctagttatc tttttatttt atatacaaaa aatccgtttc attcatcgtt
     6001 tcatcgaata cttcgctttt gattcgattg aacaattaac tcagcgttgg aaaaaaagaa
     6061 ttcattggct ttttgttcat aaaagttatt ttttagttgc agcatttttt tactgcttta
     6121 ccttcgtgca gattttcgtt ttagaaccaa aagaaggttg gaaagaaact atccaggttg
     6181 ccttaaaaat atttactcag cgacaagcac ccatgatcct gacaatcatc attattatgg
     6241 gatttttctt cttactattg ttgatttcca atcgtttttg gtacgccttg actgctacct
     6301 tgatcattaa cttactgctc actatttcga cagtaatcaa aatggaaatg cgggaagagc
     6361 ctgtctttcc ttcagattta aaaatgctga ccggattgtc agaactatta tcgatggtta
     6421 gcccagtctt attaatcgtg ggcggattga tccttctcct attattgatt actagtatta
     6481 ttgtacaacg tagactacaa aaacagtatt ccttgaagat tcattggaaa cgtcgattta
     6541 tcagtatcgc tgtattatta ggatgttttt cgggtgtctt tttcattaat cacaaaaatt
     6601 cgccttcttt cttgctgttt aatttattta aagtaaacaa gactttcttt aaccagcaag
     6661 gcgccgtaaa agataacgga ccagtcattc aattcctaaa caatatcgac attaaaatca
     6721 tggaagaacc tgctggctat tcgaaagaaa aaattaatgg aataatgaaa aagtacaaca
     6781 caaaggcaga tgaaatcaac caaacaagaa gtgactggtt aaacaatcag acgatcgttc
     6841 tcaatttaag tgaaagcttc agtgatcctg cacgtgtccc taatctgaca gtacctacag
     6901 accctattcc ttacatcaaa agcattataa cagaaggaac aggaggattg atgttatccg
     6961 tcggttatgg tggcggaaca gctaacatgg aatgggaagg actgacaggc ttgagtatca
     7021 gtaacctgtc tgcttcgtta gttacacctt atacccagct agtggagcgt cagaaaataa
     7081 gtccaaatat taccgatcta ttcgatgaaa aaatagccat ccatcctttt acagctgctc
     7141 tttatcgcag aaaacaagtg tttgataaat ttggttttga caggttctat tatttagaca
     7201 gtccggataa attaacttat acggataaaa tagaacaaag cccacgcata tcggatgatt
     7261 cagcatacaa agaaaccttg aaagctttac aaagcaatac ggaaacgtca caattcatcc
     7321 aattatccac tatgcaaaac catatgcctt atgatgatta ctatagtgaa ttgaactaca
     7381 ctgctgaagg aactgctgtc attcctaata gaagaaaaga attacaaact tatatgcaag
     7441 gccttcacta taccgataca gcagtaaaaa aattcatcca agaaatcgac aaaatcgata
     7501 aaccgattac ctttgttttt tatggtgacc atttacccgc aatctatagt gggaatgatt
     7561 cgaaaaaata tggattagaa cagcaccaga cagattattt catttataac aacaagtata
     7621 gtcgtgaaca agcagctacg ttaaataaag aagttgttgc tcctaataac tttggtgcta
     7681 tggctcttga gcatgcgaat atcaaagtaa ctccttacta tgcgttgttg acagaagtat
     7741 ctaatcatct acctgcatta acaattgatc ctacagaaag cttgtctaat cgttttaatg
     7801 gaaaacaggt ctttgttaca gaggatagtc aaattatcca agaaaaaaat ttaactgaga
     7861 aacaaaaaga aattctagaa gattatcgtc tgatacaata tgatcttgtt gcaggtaacc
     7921 agtacagtgc aacatgggcg acacaaaaaa taaaagacta acaaataaac tgagcaaggg
     7981 gttgtgataa aagacatgtc acaacccctt gtttatctca ttaatttgct tgtccataaa
     8041 cgatttgatt aaagccattt ttcaattcat tattcgctgt ctcgttgaag taaagataat
     8101 tatcgggatt cccctgattt tgttgtgtca ccaatacttt tggctgattt tgctgaaacc
     8161 cgaatagata gacgtgcttt ttcagatatg gttctgtctc tgcatcggaa tagacggctg
     8221 tgatttgata gtctgcttga cctgtcccac tttctgacca ttctgcagaa acaggcactc
     8281 caccaatagc catcttccac tccccgttca aaatagcttg agggacttta agaccatata
     8341 agtccacact catttgattc gtataacttt tgtattgttg tcccattgtt tggccccatt
     8401 tggtcataaa tgcctctaat tgtgcggatt tgtctgcatt ccacagtgtt tgttcaactt
     8461 gttcgctgct ttctgaagag gaagatgaag cctctacaga agaacttgaa gtagatgttt
     8521 gagttgtttc agtcgttttg gaagactctg acgaagtaga ggtatctgct gtttctgtac
     8581 ttgctgcagt tgacttgctc tctgtgctag ttgttgtagt agttgcaact gttgctttcg
     8641 ctgtttcttt tgttgattct tttttcgatt cattcccgtt ggtacaacca gctaacaaaa
     8701 aagctagacc aattgttact aaaatccttt ttttcaatta aaaaactcct ttcgattact
     8761 cccacttctt tattctatca aataagcaat gtgattttat tgatttttcg tattttctca
     8821 tcaatctttc aaatcttcta aaaactgaga agcacccgct gatgttgtct gtaatacatc
     8881 agcgggtgct tcttttattt taatcttcgt gattttccaa tttacctcac gctttatatt
     8941 tgtatttgaa gaaacataga cttttagtca tcatcgtctt cctcatccaa atcttgtcgg
     9001 acagtttttc cttggagtct ttgtacttgg tagatgattt ctttattgaa acttaaaagt
     9061 tcaacaagca attgttgcaa gccatatttt tcttcttttt cagctaattt gattgcccgt
     9121 gtgataaaga cattttggaa atcaaaatct tttgcaaagt tttccaacat tgttgaggct
     9181 ggtaaatatt tgttttgacc atgttcgtaa atttttccat aacgcatgac ttcttctaaa
     9241 gtagacggaa cgatttcatt ttcaatcact aactcatgag agattttatt gaaccatctc
     9301 gcttcttgat cagcatattc tgaaaaaagg gtacttaatt cttgcgaaga tgtacccaaa
     9361 acatacaatt gtgcttggat caatttcatt tcttctaatt taaaatttgc taatacatgc
     9421 cccaccatgg cgccagcagt tggtttatga tggtcgatat ctgcttgttt tgtttcttga
     9481 acaaattttt cttccactat attcattgat ttacgcctct tttacttttg ctgattggac
     9541 ttcataacct aatttttcca caactgcagc taattgttca gggtggactt catctggatc
     9601 aaagtttgct ttgattttgc tcgcattaaa caaaactttc gtattgctta ccccaggctg
     9661 ttgagaaacc gcatgttcga ttttttgcat acagcttgga caagctagtg tatctaattg
     9721 cataataatt tttttcatcg tagttcttcc ttcctttttc ttgatagtct tattctagat
     9781 tacagcttcc ataaaaaaat tgattcttat caagttttac gatttttctt ctttgctttc
     9841 gtgtccaaaa tacaataaac gcatcgcgtt aaagataacg atcaagatac tagcttcgtg
     9901 aacgaacatc ccactagcca tgtaaataaa cccggcaaat aagccgttga gtaaaaataa
     9961 gacgacacca atggcaatca ctacattttc acgggtattt aaaacagttt tcttagccaa
    10021 gcggtaggca tgaaccaacg attcaaaatt agattgcatc aagacaacgt cactcgtttc
    10081 gatagcaaca tccgttcctg atcccatagc aatcccgata tcagccgcag ccaaagaggg
    10141 agaatcattg attccatctc caataaaagc aacgcgcaat ccttgttctt ggtatttttt
    10201 cacaaattga accttttcgt ctggtaataa ttcagcatgg acttcatcaa tgcctaacat
    10261 atcagctaca taatcagcag tcatctgatt atcacctgtc agcatcacta actgttttgc
    10321 tcctgcttgt ttcaacttag ctaattgttc agcgacttct ggtcgaatca catctgacac
    10381 acctatgatt tgagtgattt ttttgtcgat tgctacaata atgaccgagc tgcctgtttg
    10441 ttgtaattga tgtaaatctt gtacttgctt tggtgtgaga gaaagtttgt gagcggcaat
    10501 caatttggtg ttgcccgcac aaactgtatg accatcaatc tcagctgtga ttccttgtcc
    10561 cttgattgtc tgattatctt tgactgctaa ttgttgaaaa tcaatgtctt tctcctgagc
    10621 ataatggatc acggcgcgcc ccagaggatg atcggataac gtttctaatc gagccactaa
    10681 agctaagctg tccccttcat cctctgtata gttcttgata gtggatacag cagtagttcc
    10741 tttggtcaat gtaccagttt tatcaaagac aaaagtatcg attttggaaa aagtatccat
    10801 tgcttcaccg cctttgacta agatgccccg tttcgctcca ttaccgattc ctgctacatt
    10861 cgaaacaggc gcaccaatga ctaaagcgcc aggacaacca agaaccaaaa cagtgattgc
    10921 taaacgaaaa tcacgactaa tcagaccaac aaccaacgca ataagtaaaa cggttggagt
    10981 ataatactga gaaaatcgat caatgaattt ttccgcatgg gatttggtat cttgtgcttc
    11041 ttctactaat tcgataattt tggcaaaggt cgtatcatct ccgaccttag ttgcttcaat
    11101 cgtcacgtag ccatctgcta aaatcgttcc agagaagact tcatctccac tgattttgac
    11161 gacttgtcgt gattcaccag tcactgcggc ttcatccgca taccctttcc cgtcaatgac
    11221 tcgtccgtcc acaggaatag ctccaccagt ttttactaag acgatatcgc cttcttccac
    11281 gtcatcgatt tccacttctt cagtcgttcc atcttcttgg acaacactag cagtagatgg
    11341 ggccatctcg gtcaaagcct tgattgactc ccgcgttttg gtcaatgttt tttgttccag
    11401 atagctacca aacaaaaaca agaatgtaac gatagctgac tccttgtatt ctccgatcat
    11461 aaaggcacca agcacagcaa tagtaacgag taaatcaatt gagataactt tgactttcac
    11521 tgcctgatac gcatggatca taataggtat gaccgcaata atagaagcta tggctagagc
    11581 aatgttatag aacaagacaa aaccagtaaa ttttccgata aaaccaaaaa taatcaacac
    11641 agctgataag gcggtgatat gatttcgatg ctcttggatc cacttttgta atttcatctt
    11701 tctccctctt ttcctttcat tcttacgaga ctaagtatag atacttatcc atcataaaaa
    11761 attgaccact atcaagttta gtcaaaaagg cggttttctt ttggtagaat acactacaaa
    11821 aaatagttag caagaagact cactaaaagt gatcttgcca actattgtcg tttctatcga
    11881 aattttcatt gaatgatttt cctgtacagc atacatcgga gatctatttc tttatctaag
    11941 atttttatct tgctaatttt ttcctttagt ctttcaaatc cacgtcgttc atagaattga
    12001 tacgtacagc cactatctgt aaaaaggaat atttcttttc ctttctctct tttttcaaat
    12061 tctgttagca acttggtacc gatgcctttt gttttcaaat caggatttgc agctaaaaaa
    12121 atgatctctc catctggcga atgcttcttt aaatatgctg caagcatttg ttcatttgct
    12181 gtctcgtaag ctcctttact ttctttagca aatatatttt gtaaaaaatc gaatatctta
    12241 acatatattt gctgtccaaa agaacgatgt ttcttttttt ctccttttat ctccgccagc
    12301 aaaacacctg ccaactcgtc ctctgcatac aaagcaagaa tttgagttgc tcgattcaac
    12361 tccatataca aaaaatatct tccgtaaaga ttcagcagaa gttcattttc catatatctc
    12421 tcaaagttca ttccttcaac agcaaaatag atagcttttt tgtaatcttt cttttctaag
    12481 ggtttaaaag ttagtttcat catttcgcct cctcaacttc aatgattgtg ttatacttac
    12541 attataatag acacagtgtc tactatctat aatcaatcat ctataagttt gtcgattaag
    12601 cgacagcttt gctttgatat gtcggataag gagataggta aatgaaaaag acagatagac
    12661 gcgtcaaaaa aacagaaaaa gctttggctg aaacactatc cacattatta gtaaataaaa
    12721 agattcaagc aatcactata cgcgaactaa cagaaacggc agatgtacat cgttcgactt
    12781 tttatacaca ttataaggat atctatgatc tatatgatca attagaaagc aacttttttc
    12841 gggatctaaa tgaaattctc tcttatgatc cagcccatag ctatgaggaa ctatacactc
    12901 gcttgattga ctatgtttac gcaaatactt ttttatatcg cttatttatc ctcaaccatg
    12961 gtgatatttc gtttcagaag aaagttaaac aagttattga agaaaactat ctgaacatat
    13021 ggctatatga agacaagaaa acgattatca cagaagaaat gagattcttt actacgtacc
    13081 acgtccaagg ttgtctatcc gttctcaatc ggtgggtaga aagcaattac aagtactcca
    13141 aaaaagaatt attagaactc ttacagaaac tgaatgatca catagagaaa atcatgcctt
    13201 gaataaaaac atactaagct tagttaagaa gaaccgtaac cttctcgtat aacaactgaa
    13261 tcttatttag ccaaattgtt tactgaagat tttttgagta aaatttctat aattcctact
    13321 tattgaaaag tagtttgtag tgctgcttct ccctctctaa gtgatatatc ttgttgtacc
    13381 aatcaaattc acctcatcat taataaatta cttttaggct aatttattac aacttatagg
    13441 ttgaagctgt aaccaattta tagtcatatt atatttttat catccagttt attgaattct
    13501 tctcgtttac ctcaattcat tttcaagggg aatca
//
LOCUS       DACDIG010000068        13470 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.068, whole genome shotgun sequence.
ACCESSION   DACDIG010000068 DACDIG010000000
VERSION     DACDIG010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.068"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(130..672)
                     /locus_tag="IV247_002201"
     CDS             complement(130..672)
                     /locus_tag="IV247_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAP8778259.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            807..1196
                     /locus_tag="IV247_002202"
     CDS             807..1196
                     /locus_tag="IV247_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778260.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1200..1964
                     /locus_tag="IV247_002203"
     CDS             1200..1964
                     /locus_tag="IV247_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP8778261.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2093..3040
                     /locus_tag="IV247_002204"
     CDS             2093..3040
                     /locus_tag="IV247_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8778262.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3170..3910
                     /locus_tag="IV247_002205"
     CDS             3170..3910
                     /locus_tag="IV247_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778263.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(4201..4869)
                     /locus_tag="IV247_002206"
     CDS             complement(4201..4869)
                     /locus_tag="IV247_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8778264.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            5003..5290
                     /locus_tag="IV247_002207"
     CDS             5003..5290
                     /locus_tag="IV247_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778265.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            5706..7244
                     /locus_tag="IV247_002208"
     CDS             5706..7244
                     /locus_tag="IV247_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAP8778266.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            7241..8206
                     /locus_tag="IV247_002209"
     CDS             7241..8206
                     /locus_tag="IV247_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8778267.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            8193..9515
                     /locus_tag="IV247_002210"
     CDS             8193..9515
                     /locus_tag="IV247_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8778268.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      9578..9682
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            9756..10499
                     /locus_tag="IV247_002211"
     CDS             9756..10499
                     /locus_tag="IV247_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8778269.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            10514..11506
                     /locus_tag="IV247_002212"
     CDS             10514..11506
                     /locus_tag="IV247_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8778270.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            11518..12255
                     /locus_tag="IV247_002213"
     CDS             11518..12255
                     /locus_tag="IV247_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8778271.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            12245..12967
                     /locus_tag="IV247_002214"
     CDS             12245..12967
                     /locus_tag="IV247_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP8778272.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(13051..>13470)
                     /locus_tag="IV247_002215"
     CDS             complement(13051..>13470)
                     /locus_tag="IV247_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAP8778273.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         4445 a         2297 c         2795 g         3933 t
ORIGIN      
        1 tgataagtat ataaagtata ataacaaatt gatgagtata tgtcgatgtt ttttaatcaa
       61 aataataaat aaaaactgtc taacatttaa aatttaattt agatattaga cagtttttaa
      121 gtttgtgagt tattttcctt gtcgttttct cactttttta gtccagaaac cacctgaaat
      181 atattttggc tcataagaaa tgataaaagc acgctcttcg atttctttga tcaggcgata
      241 cagttcacgt tcgctttttc taggagaaag tatttcgaga actagccgct caccttccag
      301 ccccattgca gaagtctgtg tcacaccata accgtgttct ctcagtatac ccggcaaact
      361 tttatcttct gcgctatttg ctggcaaaat cgttgtgacc atgatgtatc ctaatgccaa
      421 tttgtcttca atcaaaattc cgacactgat acctactgcg taacctaacg catagacaaa
      481 taaattcagt ggattatcta aacgatctaa aacaagggat aagccgacga tataaatcgt
      541 gatctcgacc atactgacaa gtggagcaat cagtcgatag cctttcattg tcaacatgaa
      601 acgtaacgtg ttcagagtga tataagcaaa attaatgagg aagatcatcc ccaacatttt
      661 tggatcaatc atttgtaaaa ctcctttcat ttatataaca acaatatcaa aaaaaatcgt
      721 aagtgaagaa aaattgaaac tttatacata caaaaaaatg ataaaatgat tataatagaa
      781 ttataaagat aggttaggtg attaaaatga aaacggtcaa tcgtgtgttt tgtgccagcc
      841 acgtgctaag tctccttctc agtatatact taggtgtcta ttcgattcaa cagccaattg
      901 aggtagagaa cattgttttt cagatgtcgc tgacagatgg tttaatttta agcgtttttt
      961 tctttttgat actgtttatt ggtaatattt tcggtagcgt tgccagtttt ttgaattttt
     1021 ctatttatcc actcctttcg ctagcattag gagtggtggg actttgttct ctgtgcttat
     1081 ttcccgagcc attcagtcct gcatttatat tatttggtac actgaacctg ttccagacaa
     1141 ctgttggagc atggttgcta tggcgatcag gaaatttaat gaaaataggt gagtaacaat
     1201 tgaatccaat tatattgaaa cgttctgtcc atcatattcc agtgttagaa gttgttctga
     1261 aagaattaag agaggaacgt attcccgttg tcatctatta tcacggatgg cagacaagta
     1321 aggagttagt tttgacgcaa ggaagaaaaa ttgcgcagca gggcattcgt gtgatcctgc
     1381 cagatgcgat gaatcatggg gagcgcaaac agccggtttc ttctattcca tcctatacat
     1441 tttgggatag tatctacgga aatctgtttg aatttgacac acttatcaag catttagaaa
     1501 aaagaggtct tctacatgat cagcttgcag ttggcggggt ttcgatgggg ggaatgacaa
     1561 catgcgcctt gttggctaaa catcctcata tagtagcggg cgtgtgtttg atgggttccc
     1621 ctgcaccttt ggaatatgga aaagaaatcg taaggcgcgc atcggaatat aattatgtcg
     1681 taccaaaaga ctattttgat ttgatttcat gggttaagag ctatgatttg tcattgcagc
     1741 cggaaaagtt agctggacgt cctctgttct tttggcatgg acaagctgac gaaaaaatcc
     1801 cttatataca aacggaacga tttgtagaaa aaaatcggca taagtcttac ggaaaagaaa
     1861 ttgtttttcg ttcctcaaaa acacagcgac atatggtaca aataccacta atggaagaag
     1921 cttccacttt tttagcaaaa aatttgataa aaaagtataa ataattgaaa cggaaaatca
     1981 ctcatgacga tagaggattt tccgtttttt attttgggct ataataaact ctgttaggaa
     2041 aatttggtat aattgatagt aagaatgaca acatgttaga aaggaagaga gcatgcccac
     2101 tttagcagat gttgcaaaac gtgccaatgt ttcaaaaatg acggtctctc gagtcatcaa
     2161 tcatccggaa caggtgactg acgaattgaa agagttagtt ttctcagcaa tggctgaact
     2221 ggattaccgt ccaaatattg ctgcaaaagc acttgtcacg aatcggtcgc agatcatcaa
     2281 attatttata ctggaagaaa tcgacacaac tgagccctat tacatgaatt tgctgatggg
     2341 aatcgctaaa cggatcggta aagcgcatta ttctctccaa ttagttacaa atgatgcctt
     2401 cgacgtcggt tcttgtgatg gatacatcat cacaggtgtc agaaaaaaag acttcgattg
     2461 gatcgaccgt ttggaaaaac cagttgtctt gtttggggaa aattctgaag gatatgatta
     2521 tgtagacagc gataatgaat atggtacagc aaaagcaacg caatatgcct tagaaagaga
     2581 ttatcaaacg attatttata tcggtatcga agtggacgag ccttttgaaa tttcaaggga
     2641 aactggttat ttatctgtga tgaaggaagt tgatcttcct agtaaaatct tgcggtttcc
     2701 aaatcattcg acagaagtag aaagatatat gacagaaaac tgggcagatt ttacagataa
     2761 aacttgtttt gtatgtagtt cagatcggct agcactagga gcagtaagag ggattataaa
     2821 tgctggagga cacttgcccg aagaatttgg tgtgattgga tttgatgggg tatttttaga
     2881 ccaaatcagt tcacctaaat tgacaacgat caagcaagat attatacgta tgggtgaagt
     2941 atgtggagaa atgttgttaa aaaaaataga agaaaaaggg gcgagtcaag gttatcgtcg
     3001 tttttttcct gaactagtta tcagagagac aacgaattaa gtcttattac tatatatata
     3061 tttatgaaat tcatcgctat tttcttaaaa taatcagcgc attgctactg aaaacaatga
     3121 atctgtgcta ttattgagaa aagttccatt tttgagaagg atggtttgaa tgaaagaaat
     3181 cagacgaaaa catttccgtt ttcctgcgta cgatgatgag ttaggcgtaa aattaaccaa
     3241 cgacaagaag cgagtattat ttgacggaca agaagatctt ttgactgaac agaatcagtc
     3301 cccgtttgaa cagatgaaag aatttcattt cacgaacacg aacaccgaga aaatccgtcg
     3361 caagcgtcgt gcaacaaatg gattgaagtc agctccgcgt aaggaaaatt tggaaaagca
     3421 taaaggaaaa ttaccggatt ataccggcca ttaccaatca gaaatcacat cgacaggacg
     3481 aaaaaaactt tttggtgatt cctctcgttc tgtcagacaa agtgaaacaa ggacagagtc
     3541 caagcatact acacgaaacg catctgttga cacgaaggtg cgggagcgtt cttattttgt
     3601 tcctaaatat gtacccgcat cgattatccc agaccctaga gaaagcaaaa tttctgagga
     3661 tcatttaatc gaatcgatga aaaaaacgga tgactcttat ctgttgttcg atacagaacc
     3721 gacgccgtat caagaaaaaa gaaatggtga gccgtctgta cgaacattca atcgaacaaa
     3781 aacgttagag gaaacaaagc agtcagaaga aaaaaaatcg aaaaaaagcc atggtatctt
     3841 agaacgctca ttacaaggat tgatcgatga tcaaggaaac tctttgggag aaaatagcta
     3901 ttttaaataa gtgatataca aatttatatt cggctacagt tggaaaggaa acgctgcggc
     3961 gtttccttat tttatactct atccgaaagt ttatatcgta tcctcaaatt tttttgagtt
     4021 taagcgaatt ttttctccca ataagtaaga aaataatggc aaaataaaaa aacaaactat
     4081 tttcgcagat cgtttgcaaa aatagtttat ttttttataa agaagatttt actgaataat
     4141 atcattttag tttacataat gatatttctg taaattaaat aaagtagtga aaaataagaa
     4201 tcagtgctct aactgttcaa gaggatcaag tggttcaaat gtttctgtag tctgttttgc
     4261 ttccaattgg ggcaaaacag atccaatttc cagatacttc actttattca atgtataaga
     4321 tagaatagaa cagctaagta atgtatacag aaaatgtgtg agatccaaat aaagcaagga
     4381 gacaactaaa ataccaaaag taatcaagaa aagacttttg ccaatcttaa tatgaaaata
     4441 tttttcaatg gcttgataaa tgatatctgt tccgcctgtg cttccgccga atcgaaagac
     4501 gagaccgcaa ccaatgccag aaagaatacc tgtcatcaaa ctattgatca gtagactatc
     4561 tacagcaaaa tgtatatgaa ggctctccca taaatgtaaa aagaaaacta gcgcaaatgt
     4621 tccgacgatc gtccggagaa aagcgtgttt tccaaaaaga aacaatgaga taagcaataa
     4681 aggtaaatta aaaagcagtg aagtgacaga aggagcgaat ccaaaaagat tatgcaaaag
     4741 caaagcaata ccagtaaccc caccatctgc cagcttagat ggaatattga ttcctgtcac
     4801 agctaatccg tagattcctg cgcctaaaat gattccgcac aattctgtaa ataaatttaa
     4861 ttttttcatt tctagcctct tttcttttga aaattttcag caacttcttt attatactga
     4921 cttatactct tacttcaaga ggaaaatgaa aaaaaattat ttttggctgt ttctttttat
     4981 ttatcaaata aggagtggaa aaatgcgagc agcagactta ttgacaattc ttacaaaatc
     5041 acccaaaaaa acagagtatc aaggagtttt tttggctcaa gaacaaaaac tggtgcctgt
     5101 gacaatggtc cagataaata aagcaaatca atttatcttt ttatttgaac acggcaaagc
     5161 accacttgct atgaaagaca tctttctttt tttgatgaag aaccgtgaga aagctgttta
     5221 ttatcagtca aaagaagaac tcattcctct ttatggagtg aaagagatag aaaataaagt
     5281 ggtgatataa gtaaaaataa gaaaacaaga cgtttatgat gtcaaaacaa ccatttttta
     5341 catttttggc aaaaaagtgt catgatacag tataatattc gttgaacagg ccaataaagg
     5401 tgttctcccc cacgagattt ctactttatt ggctgtttct ttttattaaa ataaaagttg
     5461 tgtgtgtaaa aaagatatag ctgattccaa gtgacaattt cgtatagatt tttatggtat
     5521 tcgtagtgaa aaaacgagaa ccaaaaagtt gatccgactt tgttgcttgg attctttttt
     5581 tgtcagcata tttagaggta tttttttctt ttttttgttc gtgctgtaag gttaaggcaa
     5641 gacaagttag ttgggaaaaa ggaaagaaat gtttatactt aaagaataac agaaaggaag
     5701 gtcgcatgaa taattttata caaacattaa gtgaaagaaa aggcgagttg ctggaagctg
     5761 catttcaaca tctgtccatt tctctcattt ctttgctgat tgcagcactg attgctattc
     5821 ctttagcgat ttgggcagcc aatcataaaa aaatggcaga agtcttattg cagatcacgg
     5881 gtgtgctcca aacgattcct tcacttgcct tattaggttt acttatccca tttgtaggga
     5941 tcggaactgt tccagcattg attgcacttg ttatttacgc tttgcttccg attttccaaa
     6001 acacgtatat tggtctagct gaaattgacc cgtctattga agaagcagcg gtagcatttg
     6061 gtatgtccag aatgagacgc ttgctgaaag tagagcttcc catcgcatta cctgtcatta
     6121 tctcaggtat ccgaactgcc ttagttttaa tcatcggtac ggctactttg gcagcattga
     6181 tcggagcagg tggattaggg acatttattt tgttaggtat tgaccgaaat acacctgttt
     6241 tgacattgat tggtgcgatc agttctgcct tgcttgcaat tatattcagc ggactgattc
     6301 gctggttgca gcataagaaa cctcgatatt ccatcattac tttagcagca gtggtgtgtt
     6361 tatttggcgg cgtttcttta tatcagtctg ccttgtttaa aaatgaaacc atcacgattg
     6421 ctggaaaatt aggagcggaa cctgaaatcc tgatccaaat gtataaagaa ttgattgaag
     6481 acaacacaaa aacagaagtt gaattaaaac cgaattttgg caagacgagt tttttgttca
     6541 atgcactgga tcaaggtcaa atcgatatct atcctgagtt cacaggtact gtcctagaaa
     6601 gcttggtgaa ggtacctgaa gcaatgaaaa gtgctgattt gacaaaagaa aaaacatatg
     6661 acgaagcaga agaattattg aataagcaat tcgatatgac attattaaaa ccaatggctt
     6721 accaaaatac gtatgccttg gcagtcaaga gatcttttgc gaacgaacat caacttaaaa
     6781 agatttccga tttggcagct atctctgatc agatcaaagc gggattcaca ctggaattta
     6841 ttgatcgttc agatggatat aaaggaatcc aagaagcata tggcttgaat ttttcaagtg
     6901 tacaaagcat ggagccaagt ttgcgttacc aagcaatcaa taatggagat gtcaatattg
     6961 tggatgccta ttccacagat agccagctca aggagtacga tctagtgact ctagaagatg
     7021 atcgagggtt attcccagct tatcaaggcg caccgctgat gaaaaaagat tttgctaaag
     7081 aacatcctga aatcgttaaa gcactgaata aattaagcgg aaaaataaca gaggatcaaa
     7141 tgattgatat gaattaccaa gtcaatgtcg aaaagaaaaa gccgacagaa gtagcacgaa
     7201 tcttcctgaa aaatgaagga ttagtaaagg aggaagatca gtgactaaat taatcgagtt
     7261 caaacatgtg caaaaaaagt atgatggaaa gtatgtcatc gatgacctaa atctggcaat
     7321 caaaaaagga gaaatctttg ttttggttgg tccttccggt agtggaaaaa caactacttt
     7381 gaaaatgatc aatggattat ctaaaccatc agcaggagac atctacttca aaggaaaatc
     7441 gttaaatgaa tataatctgc agaaaatgcg gtggaatatg ggatatgtgc tgcagcaaat
     7501 cgctttgttc ccgaccatga cagtcaagca aaatattgaa gtcatccctg aaatgttagg
     7561 ttgggaaaag cagaaaagag cagaccgtgt agatgaactt cttcaaaaag ttggcttatc
     7621 tcctgatatt tatcgagatc gtatgcctag agagttgtct ggaggagaac agcaacggat
     7681 cgggatcatc cgagctattg ctgcaagccc agatgtgatt ttgatggacg agccatttag
     7741 cgcattagat ccgatttcaa gaaactcatt acaagaattg gttttgtcct tacacgaaga
     7801 attgggcacg accatcgttt tcgttacgca caatatggaa gaagcaatca agctaggtga
     7861 tcgtattgca tttatgaagg atggagaaat tatccaatgt gacacaccag agcagttatt
     7921 gatgaatcct aaaaacgact acgttcggca tttctttgat gaaccgaaac agacgaaaga
     7981 gtggcgagta gaagatttag tcgtcaatgg atatttctta aatgaaatac cagaaaatgc
     8041 tcgtcaacaa gtttgctttg atacgcctat gaaagaagta tattcgcttc tttcggtgta
     8101 tccaagcatt gtgatagtag aaaagcaaag aagtattggg tcgctgacaa gtaaagaaat
     8161 ctttgctttt ttaagtcgag aggaggaagg aaatgaaaac atatgatgca atcatcatcg
     8221 gttcaggtgt gagcggctta agtgctgctt atggattgaa agaagcaggt aaaacagtac
     8281 tagtggtcga agaagatcta tggggcggga cttgtcctaa ccgtggatgt gatcctaaaa
     8341 aagtcttgtt aagtgcagta gaggcacgta atcgggtaaa acaattgagt ggaaaaggtt
     8401 ttaacgagat cccaactgcc aactgggaag aacttcaaaa attcaaacga acatttacgg
     8461 atccagtccc agaaagccga aaaaaacaat tagcagaagc tgaaatcgat catctttcag
     8521 gaaccgctcg ttttttggat gattcatcta ttgaagtcaa cgaagaagtc ttccatgcgg
     8581 attacttagt tttggcaacc gggcaaagac ctacgatctt accagtggag ggaaaggaat
     8641 acttgaaaac tagtgcagac tttttatcat tgcctgtact gcctaaagaa atcattttta
     8701 tcggtggtgg ttatattgct tttgagctag ctacgattgc caatgctgca gggagcaaag
     8761 taaccattgt ccatcataat caacggccat taaaagaatt tgaggcgtct ttggtagagg
     8821 aagccgtcca tcaaatggaa gcatctggca ttcagtttgc ttttggtgtt gaaacccaaa
     8881 agatcatttc agaaggtact cgctatcgtt tagtcggaaa agagacggaa ttagtagcag
     8941 atatgatttt ttgtgcgact gggagacagc cgaatacaga atctttagca cttgagcaag
     9001 cgaatatcgt ctttgataaa cacggaattg cagtcaatga ttatctgcaa acgagcaatc
     9061 caaaaatttt tgcgtgtgga gatattgttt caaggaaaac cccaaaattg acaccagtcg
     9121 ccacttttga aggaaattat gtagctaagc ggataacgga tgcaacaagc gaacctatca
     9181 aatatccgat tatacctacg atcgtctacg ccagtcctaa acttgctgaa gtaggagtta
     9241 caaaaagtca cgcctcttcg tcagatcaag tggtagagat ggatcttacc agttggttca
     9301 cctatcatcg tgtcaatgaa ccagttgcta aggctgaatt gacctttgac cagcaaaatt
     9361 atttgattgg tgctgcagtg attagcgagc aagcagacga attgatcgat gatttgacac
     9421 tcgtcatcaa tcaaaaattg actaaaaaag agttggatag ctatatcatg gggtatccga
     9481 cattggcaag tgatctctcc tatttattga aataatcagc atgataacta aagcgcttgt
     9541 tgctttttcc ttcttgtgct acactttaaa aaatgaatac ctgtttctgg ggatgtcgta
     9601 gacgacatga gagtaagttg tgaacttaga cccattgaac ctgtcagtta atactggtga
     9661 agggagaaac atagcagacg cttgaagcgg gttactatgt cgtgcccatt tcaagcgttt
     9721 tttgttattc atattagaag aaaggaggta acaaaatgaa gaaaaatctt tatccagcag
     9781 tcagtatggc attcatttta attatttggc agattgttgt catagtttgg aaaatacctg
     9841 cctttatttt acccggacct gctgctgtat tttctgcatt agcggcagat gcacaagtat
     9901 tactttccca ttctgtcacg acattaaatg aagcagtgct tggtttggta atagctagcg
     9961 ttctttcttt tattactgcc ttggcaatgg attattggaa gtttttagaa atgtcttttt
    10021 atccgttact agtgatttca caaatgttgc caatcatggt gttagggcca ttacttaccc
    10081 tatggtttgg tttcggtatg cttccaaaag tgatccttgt tgtcttgata agctattttc
    10141 cgatcgtagt ttctttttcg gatacattga ggaaagtatc aaaagagcaa atcatctttt
    10201 tgaaaacgat gggggcagat acagggaaga tttatcgaat atataaaata ccagcgggtt
    10261 tatccggctt cttttccggt ttaaaagtgg ctgctactta ttgtgtcagc ggagcagtcg
    10321 ttggagaatg gctaagcgct caagcaggct tagggtatta catgatacgc gtaaaaaaca
    10381 gctaccagat cgataaagtt tttgctgcga tcatttgtgt cattctattg agtttattgc
    10441 tgaatggcgc ttgttcatta ttgaaaaaag ggtattatcg tatgctttat attttttaag
    10501 agaaggggat caaatgaaaa aattatggct tttattaccg ctactgttgc ttttatccgc
    10561 ttgtggcact gcaaaagaaa caagtagcaa acaagaaata aaggatttga aaaaagtgac
    10621 acttgttttg gattatgtac cgaatactaa tcatactggc atctacctag caaaagaaaa
    10681 aggctattat aaagaggctg gtttaaatgt ccaaatcatc gaaccaggag ataattcgac
    10741 aagtattggt ctagtaggtg cggacaaagc acaatttggt gtaagttatc aagaagatgt
    10801 cacttatgct cacgcagatg gacagaatat accagtcaaa gcaattgcta cagtgatcaa
    10861 acataatact tctggattcg ccaccctatc agacagtaac atccattcac caaaagattt
    10921 tgaaggaaaa acatatgcag gttggcaaag tccaagtgaa gaagctgtct taaaagctgt
    10981 gatggaaaaa gacggcgggg acttttcaaa actgacaatg gtaggaagca atggagaagg
    11041 gccagaaagc ttaggaaaat cctcagatat ccaatggtac tttgaaggtt gggatatgat
    11101 caaagcaaaa gaagcgggta ttgaactaaa ctatatccca ttgaaagaat tagatgaacg
    11161 gttagattac tatactccgg taattattac gaatgatcaa ctgatcaaaa gtgatccaga
    11221 attggtacag tcttttatgg atgccacgaa aaaaggctat caggaagcta ttaaggatcc
    11281 aaatgacagt gctaaactgt tgcaaaaata tgcaaaagaa aatgatcgga cgtttttaga
    11341 agaatcacaa gcattcttgt caaaaaatta tacggatgat cctgagaatt ggggattgat
    11401 ggaagaaaaa gtttggcgca attatacagc ttttatgcag gaaaacggat tgatcaagca
    11461 agatgtacct tcgcaagaac tattcactaa tcagtttata aaataggagt gatagatatg
    11521 tcaatccaaa tcagccactt gaatgtagtc attgaaaacc tgaaaatatt agaagacatc
    11581 catttcacta tccctaaaga tacctttaca tcgattgtcg cgccaagcgg agccggtaag
    11641 tcaacattgc tgaaaacttt aacaggagtc cagcctatga cttctggaac gatcaaagta
    11701 gacgatcaaa aagtaactgg tctccataca gcatttagtt atatgccgca agaagatatg
    11761 ttacttcctt ggctgagcgt ctatcaaaac gttacgttat atcaaaaaat caatcatcta
    11821 acaatcaatg aagaacaagt aagggattat ctggatatct ttgggttaaa cggctatgaa
    11881 cattttttgc cggaacaatt atctggcggg atgaagcagc gaacggcctt gttacgaaca
    11941 atcatgaatc cttcttctta cttgcttctt gatgaaccat ttggagcgtt agatgctatg
    12001 accagagggc agatgcaaga ctggatgttg aagctgccta aaaaagcaaa gcggactact
    12061 ttacttgtga cgcatgatat cgaagaagca atttatttat cagacaggat tcttgtgctt
    12121 tccgcccgtc cggcacatgt gattgctgag attcaagtgc ctgaaaaacg acggtcacgt
    12181 gaatggctac tgcagcaatc agagttgaag caaacgattt atcagttatt ggcaggtgaa
    12241 ttagatgtta agtgatgctc attgtcattt agatgggagt cagagtttag cactccttca
    12301 gcaggaacac acgattttga cgatcatcaa ttgtgattcg ccagaagaat ggaaggaaaa
    12361 cagacagctt gcagcaagta agactcaagc cttaagttac ggtatccatc cttggaaagc
    12421 agatagctat acgtttgaac aagttgagcc ttttttgaaa aaagcaagga tcatcggtga
    12481 aatagggtta gataatattt ggacgaatgt accgatgact acacaaaaaa aagtatttga
    12541 gcgacagctt gcttttgcag ccataaatga gaaacctgtt gtactgcaca caaaaggatg
    12601 cgaaaaagag atcttaaatt atatccaagc ttaccctaat cactatttga tccattggta
    12661 ttcttctttg gactatcaac aagactatat tgatttaggc tgttatttca gcattggtct
    12721 tgatttgaaa aagaatccag cagtctggca actagctaaa gcagtcccaa tcgaccactt
    12781 acttattgaa acggatggac tacatgcagc agagtgggct ttagggggga ttttttcaga
    12841 aaaagattat gtctcactgc ttgaagaaca gttaaagaca atcgctgaga tcaaagagct
    12901 atcagttgaa gaagttaaaa aaaatactta tcaaaacttg gaacgtttca ttagattagg
    12961 taaataaaaa agagatgtga cacaactagt gtcgcacctc tttttggctt tctttgtata
    13021 aaagtaaact gtttgattaa taaatgaatc ttagtaccaa ccgtttgctt cccagaatgc
    13081 tttcgccgct tcccaagaac cataacgtga agtaacatat tgttctgcta ctctttcttg
    13141 gtttgcagca gagtagtcac catttaagta agaagaatct aattggtaac gtccgatgta
    13201 acgtccgttc gttgctgtgt aagaaccgct tgattctttt tgtgcgatcc attcttttgc
    13261 agagcttgtg cttgctggag tagcagcagt ttctgttaca gcaggagcag gttgtgtttc
    13321 agcaacaggc gctgcttgtt tttccgtttg aaccgtttct tgtacaacag gttgttcagc
    13381 ttggactggt gcttcttgaa cggctgcttg aggttcagca gctgcttgtg cagagccttc
    13441 tgttgggatt gttaattgtt gtcctgagta
//
LOCUS       DACDIG010000069        13250 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.069, whole genome shotgun sequence.
ACCESSION   DACDIG010000069 DACDIG010000000
VERSION     DACDIG010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.069"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..913)
                     /locus_tag="IV247_002216"
     CDS             complement(<1..913)
                     /locus_tag="IV247_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAP8778274.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1341..1904
                     /gene="efp"
                     /locus_tag="IV247_002217"
     CDS             1341..1904
                     /gene="efp"
                     /locus_tag="IV247_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAP8778275.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2390..3649
                     /gene="lysA"
                     /locus_tag="IV247_002218"
     CDS             2390..3649
                     /gene="lysA"
                     /locus_tag="IV247_002218"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAP8778276.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            3636..4436
                     /gene="dapF"
                     /locus_tag="IV247_002219"
     CDS             3636..4436
                     /gene="dapF"
                     /locus_tag="IV247_002219"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAP8778277.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            4439..5320
                     /locus_tag="IV247_002220"
     CDS             4439..5320
                     /locus_tag="IV247_002220"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAP8778278.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            5317..6033
                     /locus_tag="IV247_002221"
     CDS             5317..6033
                     /locus_tag="IV247_002221"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAP8778279.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            6039..6734
                     /gene="dapD"
                     /locus_tag="IV247_002222"
     CDS             6039..6734
                     /gene="dapD"
                     /locus_tag="IV247_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAP8778280.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            6750..7751
                     /locus_tag="IV247_002223"
     CDS             6750..7751
                     /locus_tag="IV247_002223"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAP8778281.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            7748..9109
                     /locus_tag="IV247_002224"
     CDS             7748..9109
                     /locus_tag="IV247_002224"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAP8778282.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            9069..10220
                     /locus_tag="IV247_002225"
     CDS             9069..10220
                     /locus_tag="IV247_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAP8778283.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            10195..11364
                     /locus_tag="IV247_002226"
     CDS             10195..11364
                     /locus_tag="IV247_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP8778284.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            11539..12918
                     /locus_tag="IV247_002227"
     CDS             11539..12918
                     /locus_tag="IV247_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP8778285.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
BASE COUNT         4417 a         2307 c         2885 g         3641 t
ORIGIN      
        1 agtaaatgtt ttttgataaa tagcggtctg ctgcgtctgg aaaaatcgta acgacacgat
       61 gaccaggtgg taaacgacga atttctctta atgctgctgc aaaagccgca ccactcgaac
      121 ttccaactaa tagtccttgt tctcgtgcta actgcctagt atagttaaac ccttctacat
      181 cagagattgt ctcgatctga ttgattgaaa ggggagaaag aaacggcgga atgaattcta
      241 caccgatacc ctcaatttcg tgaggggctg gatcaccacc atttaacaca gagccctctg
      301 gttctacacc gattattcga atgtcgggat atttttcttt caaaaaggta gaagttccag
      361 caaaggttcc accactccca attcctgcta caaaactatg gatattttct ttgatttcat
      421 gaaagatttc tggccctaac gtatgatagt aagcagcagg attatcttta ttttcaaatt
      481 gaagaggcag atagctgttt gatatctcct ctgcaagttt tttacttttg ctgatggcac
      541 cgatgattcc ttgctcacta ggagaatgga tgatctttgc tcccaatgct ttcatcaatt
      601 gttgtttttc taagctaaac ttctctggaa caacaaaaac cgttcttaat ttgtatgtga
      661 gtgcggccag agcgatccca attcctgtat ttccggcagt tggttcgatg atcgtcgttt
      721 tttcattgat ttttccttgg gcaaatcctt ctttgatcaa ataactgccc aatctatctt
      781 tgatacttcc tccaggattt aaatattcta gttttgcata aatagcagaa cctttaggca
      841 tagaatagtg ctgattgtca aagcgataaa gcggtgtgga accaatagct tctgtaattg
      901 tcgagataat catgtgggcc tcctaaaaaa agataataaa aaaaggagct gactattaaa
      961 tcagctcctt gaaagacata gagaaaaatt gagtagataa aaagataagt tacctggtac
     1021 tcattctatg aagggcaatt aatagacatg acaaatagta tgctgctttt atgcaccata
     1081 caacagcaac gattcaattg tttttggttg ctgactgtca tgtgtatccc tccaagtaaa
     1141 atttttatta acgatagcat gaaaaaaaat aaaagtcaaa ggcttgaaag aaaaaaatat
     1201 ttcgatagca tcgtttaccc gtgtttattt ttctgacaaa taggcatgag accttaaaag
     1261 atagagaata ctctagtttt ctggccggtt gtctgataca ataaagttga ttatgataaa
     1321 gaaaagaagg agcaaaaata atgatctcag caagtgattt aagagccggt atgactttcg
     1381 tacaagacgg caaactgatt aaagttctgg atgcaagcca ccataaacct ggtaaaggaa
     1441 atacagttat gcgtatgaaa ctgaaagatg tccgttcagg tgcaacatac gacacaacgt
     1501 tcagaccaga agaaaaattt gaaaaagcac atatcgacac aaaacctgta caatatttgt
     1561 acacgatgga tgatacagct ttcttcatgg atttggaaac atatgagcaa tatgaaattc
     1621 ctgtagaaac agtagaagaa gaaatgaaat atatcctaga aaacatggaa gtaaaaatcc
     1681 aatttttcgg tagcgaagtc atcggcgttc aattaccaac aactgtcgta ttacgtgttg
     1741 tggaaacaca accttccatc aaaggtgcaa cagtcactgg ttcaggtaag ccagcgacaa
     1801 tggaaactgg cttagtagtc aatgttcctg acttcatcga aacagatgaa ttgttggaaa
     1861 tcaatacagc agaaggttct tatgtaaaac gtgcgtcaaa ataattcgct aacggattga
     1921 taagaatttg aaaacagagc ttgacctctt gaaaggcaga tcatcggatc tgcctttcaa
     1981 gaggtttttt gtggaatcag caaattgaaa aataaaaaag tatttgattt tatttatatt
     2041 ttgaaagatg aatggcaaaa gctagagaaa gaaaaaactg cacttattta tgaagaaatt
     2101 gaaatgattc ataaaattga aaatatccga actattccga actatattga agcgaaaata
     2161 ttttttcatc actaaatagt gttgaattta tgtttttatg tataaatagc ttctaatagt
     2221 tcgggtattt tgtttttata accattttta ttcttatttt taaaaaaata tttcgaaata
     2281 tactagacaa ttcagaaaat tttgtttaat attattccaa taaaattcaa atctttatca
     2341 aatgaaattc caataaaaac ggaaaaaaag tgaaaagagg aatgaaaaaa tgacagaatt
     2401 gataattggc ggagtcacag caagcacatt ggcagaaaag tataagacac ctctgtatgt
     2461 gtatgatgaa gaaaagatga gacaaacgat gctgtcattc aagcgaggct ttgtatcgaa
     2521 acgttttgat acaaaagtgc tttacgcctc taaagcattc caaacgattg agatgctgca
     2581 tttagttcgt gaatatggat tcgggctaga tgttgtcagt ggtggggaaa tctataccgc
     2641 tttgcaagct gattttccta gagatactat ctacttccat ggtaacaaca agacgccaga
     2701 ggaactgatc tatgcacttg aaaatgattt gttgcatatt gttgccgata atttgatgga
     2761 agttgaatta ttagcagaat taagccaacg ctatttaaaa aaaatctgta tcatgcttcg
     2821 gttgaatgtc ggagtagaag cacatactca tgaatatatc gtcactgcgc atattgattc
     2881 aaaattcggc atgagctatg aaagtaaaga gtgccaacaa gcccttcagt tgataaaaga
     2941 aagcagctat ctagagttag aaggatttca tgctcatatc gggtcacaaa tttttgatgt
     3001 tactgcttgg ttagctgaaa tcgacaaatt gacgaattac ttaaccgatt ttgaccaacc
     3061 tctttccttg aatcttggcg gagggtttgg gattcgttat acacaaaatg atcagccgat
     3121 cccagtcgaa gaggcactaa aaaaactgat tgaatatacg gaagaagctt tggaaaaacg
     3181 aaaactgacc attcgtcaat tactgatcga accgggtaga agtattgttg gagaagctgg
     3241 tacaacacta tacacgattg gtttcatcaa aaagacacca cataaaaaat attactttgt
     3301 agatggcggg atgacagata atatccgtcc agctctgtat caggcagaat acgattgtga
     3361 tttagcagca aaactgtcgg aagaaaaaac agagaaagtg acgattgcag gaaaaatgtg
     3421 tgaatcaggc gatgtggtaa ttaaagaagc ttatttgccg aaagcagaat cgggtgatct
     3481 tttagctgtt tattccacgg gagcttatgg atactcgatg agcagcaact acaatcgagc
     3541 gacacgacca gcagttgttt ttgtcaataa tggccaatca agactgattg ttcgcagaca
     3601 agcattttct gatctactaa ggggggaagt ttcctatgat ccttcataaa tataatggct
     3661 gcggaaatga ttttatctta ttagattacg cagaagacac agattatagt caattagccg
     3721 cttctttatg cacaaaagaa aaatatgata tagacggatt gatcgccgta aaaatcgatc
     3781 cgttggaaat gatctattac aacaaagatg gcagccgcgc gccgatgtgc ggaaatggca
     3841 ttcgctgttt tgctcgatac gtaaatgatc agaacatgat gaatgaacag gaattcaacg
     3901 tagagacatt agcaggcaca agaaaaatta cggtgttgga tccctcacct ttttattgca
     3961 gcgtagatat gggagtgcca gatgattctc cggaaaaaat caaagctaat caaacttatc
     4021 caataaaaaa ccaggaaata gagatcaatg gtgaaaaagt aacgatcact agtttatttt
     4081 taggaacgat tcataccgtt gtttttgttg aaaatgcgcg tgaggaagtc tggagaaaaa
     4141 gaggtgaact gatctgtcat catcctttgt tcaaagaaaa aaccaatgtc aactttgtag
     4201 aagtagtcaa tgaacaagaa ttgatcgtcc gtacctatga aagaggagta ggttggacac
     4261 ttgcttgcgg tacaggttgc tgtgcctctt atgtggttgc taaggaaact gggaaaattt
     4321 cagcagatca ggtagttgtt cacttggaac aaggagactt gatgatctct ggtgaggatg
     4381 cgatacaaat ggctggccca gcagttcatg aatggacaag aatattggag gaatgaagat
     4441 gataaaaggt tcgatcgtgg cgctgatcac gccgatgaat gaagagggat cagtagatta
     4501 tgctggtttg gaaaaattga tccaattcca tttagatgaa caaacagatg gattattagt
     4561 tttaggtaca acaggagaat cttctacgct cacacaatct gaggaggagc agatccttca
     4621 actgacagta aagaaagtgg ctggccgtgt gccggtgatt gcaggagctg gaacgaataa
     4681 tacgaaagaa acgattgaaa aagcgaagca ttttgcttct ttgggggcag acgcactttt
     4741 ggtcatcacc ccttactaca acaaaacaag tgatgcagga ctagctgctc attttacagc
     4801 aattgcagaa gcctcaccga ttccgcttat tttgtataat gttccttcta gaacaggcat
     4861 gtcgattcca atccatgtat tagtaaattt agcagaacat cctaatatca ttggtttgaa
     4921 agaagcctca ggagatatgg cttatgtgat ggatgccgct cggttgattg gagaagaatt
     4981 cttcctttac agtggaaatg atgatttgat cctaccagta atgagtgtag gaggaagcgg
     5041 tgtaataagt gtatgggcga atatccaacc taaaatagtc catgaattag taaaagatac
     5101 gcaggacgga agatggcagc aagcaaaaga aaagcaatta aatgcacttg aactgatcca
     5161 tgccttgttc agtgagacca atcctattcc tgtcaaagca gccatgtcat tattagactt
     5221 accttccgga cctctacgcc tgcctttagt ttctctttca aaagagaaaa agaaacaatt
     5281 agctcagctt ttgttaaaag aaaggacggt tgaaaaatga aaatcggact gattggcagt
     5341 ggacagatgg gaaatagagt cgaagaagtt gcacaagaaa gaggagatac ggtccttctt
     5401 ttacggacgg cacctaaaga agcaactacg cttgctttta cggaactgcc agaactgctt
     5461 attgattttt ctcatccaga taatttagag aaaatccttt cgtatagctt atcatatcaa
     5521 cttccgttag tcattggcac gacaggttat acacaatcac agttccaaga aataaaagaa
     5581 catgccaaac atgtacctgt gatgtatagt gctaattttt cgttcggcat catggtgatg
     5641 aatcaactaa tcaagcaagc agctgcaatg ttgcaaggct ggcagatcga actgattgaa
     5701 aaacatcata gtcgtaaaaa agatgcacca tctggtactg caaatatgct gcttcaaaca
     5761 atccaagaag tgcaaaagct gcagcccgta tacaactggc aagggaaaaa aagagaagaa
     5821 aaccaaattg gtgtacacag tattcgtgca ggttctttac ctggtgaaca tgatgtattg
     5881 tttgctgcta ctgatgaaat atttacgatc aaacatgaat ctttttctaa tcgaatcttt
     5941 gctgcaggcg cagtagaagc agcggactgg ttgaaagatc agtcaccagg ttattacaaa
     6001 ttagaagatt tattaatgga caaaggggtg taaaaaacgt ggaagctcaa gaaatcatcc
     6061 gctacattca aacggcatct aaaaagacgc ctgtgaaagt ctatctgaat gtaaccgagc
     6121 caatcgaatt taaagaaagt aaagtttttg gtgaaggaag cagcttcgtt gtttttggtg
     6181 actatgagac gattgctccg gttttagaag ctgaatcaga aaaaatcatt gaaatcgaaa
     6241 tcgaaactgc tgcaagatac agcgcagttc ctttgctaga tattaaaaaa attaatgctc
     6301 gtatcgaacc aggtgctatt atcagagatc aagtgtcaat cggcaacaat gccgtgatca
     6361 tgatgggagc gattatcaat attggtgcag taatcggtga aaacacgatg attgatatgg
     6421 gtgctgtctt aggtggacga gcgacagtgg gcaaaaactg ccatattggt gcaggagctg
     6481 tcttagcagg tgtgatcgaa ccagcatcgg ccaaaccggt tatcgtagaa gacggcgtac
     6541 tggtaggtgc gaatgcagtg attgttgaag gtgtccatat cggaaaagat gcagtcgtgg
     6601 cagcaggagc agtggtttta gaagatgtgg cagcagaaac tgttgtcggt ggaatacctg
     6661 cacgtgtatt aaaaataagt gatgatcaga caaaagaaaa tacagcattg attgctgcgt
     6721 tacgcgaatt atagaggaga tggagaaaaa tgcgcgaaat agatacctgt attgtggggg
     6781 caaccggatt agtcggccaa acgatgctca aagtgttaga agaatatgat tttccagtag
     6841 gacgattgaa attattagct tcaaaacgtt cagccggtaa agtttgtgta ttcaagaaaa
     6901 agaaacacac tgtacaggaa ttgacagaaa cttcatttga aggatatgat ttagcgctat
     6961 tttctgcagg tgcttctgta tccaaagagt ttgcgccaat cgcacgaagt cagggactat
     7021 ttgtcataga taattcctca gcatggcggg aagattcgga tattccatta atcgttccag
     7081 aggtcaatct ggaagactat acattgaatc ggctgattgc aaatccaaat tgttcaacca
     7141 ttcaagcggt acttccgctt aaagctttac aagaagcctt tggtatccag cgagtcaatt
     7201 attcgaccta tcaagcagtt tctggttccg ggcaaaaagg cattgaagat cttcatcgta
     7261 cgcaaaatga cgaggaacca gttttgtatc cttataatat tagccaaacg gtgattccgg
     7321 aaatcgatca gcctttggaa aatgggtata caaaagaaga acagaaaatg ataaatgaga
     7381 cccgtaagat tctgcatcag cctgctttgc cagtatcagc aacctgcgta cgtgtgccaa
     7441 tcgaaaatgg acatggagtc tctgtggcag ttcaactgca aaaagagttt acgacggaag
     7501 aaattagaga gtgtctcagc cagtttccag ggattcaatt agaagatgat ctagcagcac
     7561 ataaatatcc gacgagcatt cttgctagag gaacagatat ggtttatgtt gggaggatca
     7621 gaaaagacac ttctttagag aacggattgt tgttctatac gactgccgat aacatacgca
     7681 aaggagccgc tgcgaatgcc gtacagattg ctgcagcttt aatgcagaaa atagcggagg
     7741 tggcaagatg agtaatacgc tgaaagtagc gaaattcggc ggaacatcga tgtctcatga
     7801 agagcaattc aaaaaagtcc gtacaattgt tctttcagat cccaatcgcc aagtgatcgt
     7861 tgtttctgct ttaggcaaac gtacgtcaga tgatgacaaa gtaacagact tactttattt
     7921 gatttatgct taccttcagc atcatgttga ttggacaccc ctttggaaaa ggatttgtga
     7981 tcgtttcatt caagtaagag atgttcttgc cctgcagtta cctattgaag atgaactagc
     8041 acgcatacag aaacaactgg aagacgaaaa gataactgaa gattatttag taagtagagg
     8101 tgaatatctg actgctcaac tgatggctga gtatcttggt taccagttta ttgacgcaaa
     8161 agacctgatt ttctttgact atgatgggaa aatcgatcag gaaaaaacag cacaggaaat
     8221 cacaaaagca tttaaaagat atccgaaaat ggttgttcca gggttttacg gggcgaaccc
     8281 tgcaggaaaa gaaaaattat tgggccgtgg gggaagcgac attaccggcg cgatactggc
     8341 gaagatgttg caagcggaaa gttatgagaa ttggacggat gtttcaggaa tcatgatggc
     8401 tgatccgcga atcatcgatc atcctaaaat catcgaggaa atctctttta gagaactgag
     8461 agagatggct tatatggggg ctaacgttct tcatgaagca gctgttttcc cagttcaaga
     8521 ggctgatatc cccatccaaa taaaaaatac caatgatcct ttcgctcaag ggacaaaaat
     8581 atcgaatcac gaaatagaaa aggaaaatgg actgacagga atagctggac gcaaggattt
     8641 tctttccatc actttattca aacgtcatat gtccgatgaa atcgggttta tctggaaagc
     8701 aatgagcatt tttgctaaac atgagatatc aatcgaacat attccttcag ggatcgacaa
     8761 tatcggagtg atcgtctcag cagaagcgat tgccgataag ttatttctca tcacgaaaga
     8821 attaaaagaa acacttggcg tggaagaaat cgtcgtgatc gaagacttag ctttgatctc
     8881 tgtcgttggg ggtccgcata aagaattgat tggattatct gggaaagtgt tatcgatatt
     8941 gaacaaatta gaaattcgca catcgatctt atcacaagga gcacaggagt tgaatctgat
     9001 catcggtgta ccgaataatc aatatgaaac agtagtgaag ggtatctatg aagggatggt
     9061 gaatacggat gctgaaagaa tatcgaatgg cactccatca aattcctgaa atcgggtttc
     9121 atgaattcaa gacaaaaaaa tatttatatg aacaagtaaa aaacagcgag gccgttatcc
     9181 atgaaatcgg cgaaacagga ctactccttt attttgataa tcagcaagaa gaaacgattg
     9241 cttttcgaac agatatcgat gcactgccag tcaaagaaga aacgaatctc tcttttgctt
     9301 ctaagcatga aggttatatg catgcatgtg gtcatgacgg acatatggcg atgcttttag
     9361 ggttgactga ctttataact aaacacattg aggatcttat ttataatgta gtactgatct
     9421 atcagccatc agaagaaatt tccggtggcg cacaaagcgt tattcagtca ggattgttgg
     9481 ataaatatga agtacaagca atatttggct ttcatctatg gcctggacta gctaagggag
     9541 aagttttctc tcgaccagga cctttgatgg cacaaagcag tgaaacagac attatcatta
     9601 aaggccagtc tgcacatatt gcttctagtg aaaaaggaat cgatagtctg gaggcagctg
     9661 tacgattttt gaagaatgtt tatgattttg accggcaatt accggcagag tggaatcatc
     9721 taatgaaatt tggccaaatc caaggtggaa cgatccgaaa tgttttagct ggagaagtta
     9781 ttgtatccgg aagtatccgt tcttacagtt cagatatgca aaattatttg aagaggggat
     9841 tagaccattt agcagaacag tttcactcta cagctactat ttcttttcgc tataatgacg
     9901 gctatccagc tgtaatcaac catagaggct tatatgcagc actgttagat tcaaacaaac
     9961 tacatgaatt agatgctcca gttcttcaag ccgaagattt tggcatatat acgcaacaat
    10021 atccatgtgt atttttcttt ttaggtgtcg gtgatacccc tgctttacat gatccgacgt
    10081 tcgattttga tatggatgtt ttagaaaatg gtgtagaatg gtacaaaacg attcttttta
    10141 cagaaaatct tcttgtaaaa atcgatagca gttcaaaaga ggagcgagta aataatgatg
    10201 aatttatcca atcgatttag tcctaggctt tctagaatag aagtatcaaa aatacgacaa
    10261 ttcgatcagc aaatatcgtc gataccagat gtgatcaaac tcaccctggg agaaccggat
    10321 tttccaactc ctgaacatgt caagcaagca ggaatagcag cgattgaaga agacttttct
    10381 cactataccg gaatgagagg attagaggaa ctgcgggaag cggcttgtat atttcagcaa
    10441 cagcgttatg gattaactta tgatccgcaa acggaagttt tgacgacagt tggtgcaacg
    10501 gaagcgattg cctctgcgtt gttatctgtt ttggaagaag gagacaaggt tcttattcca
    10561 gcgcctgctt attcaggata tcagccatta gtggaactag ctggtgctga attgatcccg
    10621 atagatactt ctgacacagg gtttgtctgt cagccggaac aatttgaacg agcatttgaa
    10681 caatatggat cagctgtcaa agcagtcatt ttaaactatc ctaacaatcc tactgggacg
    10741 acattgtctg caaaacaatt gaaagcaatc gctgaggttt taaaaaaata tccggtattt
    10801 gttataagtg atgaagtata tgctgaattg acttatagtg gaacgcatat gtccatagct
    10861 acttatcttc cagaacaaac aatcgtgata agcgggttat ccaagtcaca tgcgatgact
    10921 ggttggcgag tgggctttat ttttgctcaa aaagccctta ttgacgaact aattaaagtc
    10981 catcaatatc tggtgactgc cgctacaacg atgtcgcaaa aagctgcggc tgaagcgcta
    11041 ataaatgggg tgaaagatag cgagaatatg aaagagcaat atcaaacacg aagagactat
    11101 ctggtccagc aattggcgcc tttaggattt gaagtgaccc agccaaacgg tgcattttac
    11161 ttgttttgca aactcccagc tgccattcaa actgactctt ggcagttttg tgtagaatta
    11221 gcggaacagg caaaagttgc gtgtatccca ggaattgcat ttggcccaga aggagaaggt
    11281 tatatccgga tttcttatgc tagcagcatg gaaaaattac atgaggcctg caaacggatc
    11341 aaagactttt taacagaaca gtgattcata gattttatca aaaaaacaat cgttaatttt
    11401 gcggtatatt ctgcaaggag acgattgttt tttaaattta gttcaaatga acgcgtttct
    11461 tttgattcat aactaatgta atgttaagtt taaattgttt agaatgattt taaaagaata
    11521 ttcaaggagg tttcatcaat ggaaaaaatc aaagttgtca ttgtaggtgc atctcatgga
    11581 gggcatcaat cgatcttgga actattgaca cgatacggtg aaaatgtaga tatcacgcta
    11641 tttgaagcag gagactatat ttccttcatg tcatgtggta tggagctgta tttagaagac
    11701 aaggtaacag atgtaaatga tgtacgtaat ttcagaccag aaaactttcc gcaatccaat
    11761 gttcatattt taaataatca cgaagtaaaa acgatcgatg ccgataaaaa aacagtaacg
    11821 gttactagcg catcagataa cgaaacaaaa gaatatcctt atgacaaatt gattttgagt
    11881 tctggtgtca aaccaaacaa tctgccagta ccaggagcag atctagaaaa tgtctacttg
    11941 atgcgtggat acaattgggc aaccaaaatc aaagaaaaat taaatgatcc ggctattaaa
    12001 aaaatcgctg tcgtaggtgc gggttacatt gggatcgaag cagcagaagc aagccgaaaa
    12061 gctgggaaag aagtcgtatt gcttgatgtg atcgatagac ctttaggcac ttatttagat
    12121 gctgagatga cagatatttt agaacaacac ctaaatgaaa acggtattga agttttgaca
    12181 aatgcaaaaa tcgaagcttt tactggtaac ggaaaagttg aggcaatcaa aacaagtgaa
    12241 aaagaaatcc cagcagatct agtcattcaa gctgccggtg tgaaggcaaa tacagaatgg
    12301 ttaaaaggga tcgtcgactt agatgaacgt ggctggatcc aaacaaatga atacttgcaa
    12361 acgaatcttc cagatgtgta tgcagtagga gatgctacac ttgcttattc tatcccagca
    12421 cgcaaaaaaa tgccgatcgc tttagcgact gttgcacgta gagaagcacg ttatgtagta
    12481 aaacatcttt ttgaagaagt accaaacaaa ccgttcggcg gagtcgtagg ttcctctgca
    12541 ttaagtgtct ttgactatca ttttgcagca agcggattga atagctttac agcagcaaaa
    12601 gcaggagtag atgtacgagt gtcttattat gaagatacgt tacgaccaaa atatgtacca
    12661 gaagaacaag gaaatccaaa agtgtccatc caattattct cggatccaat gacccatcaa
    12721 ttgctaggag gagcagtatt atctacctat gatgttacag gtcaaggaaa tgtattggca
    12781 ctagcgatcc aaaacaaact gactttagaa gaactagcag aagctgactt cttcttccag
    12841 ccaggctttg accgacaatg gagcatgttg aatctagccg ctcaacaagc gttaggagaa
    12901 gaaccatttg tagaataaga aaacgaaata tttgaaaaga ggttgtgaaa aagctgcctt
    12961 gcatcaagta ataagaacag ccaagtaaaa acagcttctt atgtttttaa cttggctgga
    13021 cttattaccg cagatgcaag cttttgaaca ccgtttattt tcaaaaaagg gactgtgaca
    13081 cgatttgtgt accgaccccc aaaagttaga ccaaaaaatc taacttttgg aggtcggtat
    13141 tttttatggt taaatatact tttgaactta aattaaagat cgttcatgat tatttggatg
    13201 gcaaaggcga ttggaccgta aaaatccaga tcaagagatt gaagcggaaa
//
LOCUS       DACDIG010000070        13109 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.070, whole genome shotgun sequence.
ACCESSION   DACDIG010000070 DACDIG010000000
VERSION     DACDIG010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13109
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.070"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            74..1813
                     /gene="yfcC"
                     /locus_tag="IV247_002228"
     CDS             74..1813
                     /gene="yfcC"
                     /locus_tag="IV247_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP8778286.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            1970..3154
                     /gene="iadA"
                     /locus_tag="IV247_002229"
     CDS             1970..3154
                     /gene="iadA"
                     /locus_tag="IV247_002229"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAP8778287.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3265..3468
                     /locus_tag="IV247_002230"
     CDS             3265..3468
                     /locus_tag="IV247_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP8778288.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3569..4231)
                     /locus_tag="IV247_002231"
     CDS             complement(3569..4231)
                     /locus_tag="IV247_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8778289.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4244..5746)
                     /locus_tag="IV247_002232"
     CDS             complement(4244..5746)
                     /locus_tag="IV247_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8778290.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVTVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6294..7973
                     /locus_tag="IV247_002233"
     CDS             6294..7973
                     /locus_tag="IV247_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP8778291.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8100..8579
                     /locus_tag="IV247_002234"
     CDS             8100..8579
                     /locus_tag="IV247_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAP8778292.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8798..9478
                     /locus_tag="IV247_002235"
     CDS             8798..9478
                     /locus_tag="IV247_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAP8778293.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9486..10817
                     /locus_tag="IV247_002236"
     CDS             9486..10817
                     /locus_tag="IV247_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP8778294.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10832..11794
                     /locus_tag="IV247_002237"
     CDS             10832..11794
                     /locus_tag="IV247_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAP8778295.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11905..12243
                     /locus_tag="IV247_002238"
     CDS             11905..12243
                     /locus_tag="IV247_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAP8778296.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12322..13029
                     /locus_tag="IV247_002239"
     CDS             12322..13029
                     /locus_tag="IV247_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAP8778297.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4163 a         2317 c         2878 g         3751 t
ORIGIN      
        1 ttactgtata attcatttga ttatccattt tttattcatc aaaattactg aaaaaagaaa
       61 ggaagaaata aatttgaaga aaacaaattc ttcaggaaaa ccgaaacgcg acttcactaa
      121 attatcgaca ccccatacgt atgtcattat ttttggtgta gtcatctttg catggatact
      181 gacttttgtc gttccagcag gaaagttcag cacgcaagat attgaataca aagatgcaaa
      241 tggcgaaaca agtactcgta ctgttttacg tcaagattcc ttccgttatg cttacgagct
      301 ggataagtcg tatgtgtttg atcaattaga agaactgcaa gatcatccag cagaacgtga
      361 aaaactggat gttccggaaa aaggattgga aaaagtcatt gctgacggag aaaaaaatct
      421 tacgcaggaa aaactagatg aaatctcctt gacagatgat gtgctttacg atcagtacgg
      481 cgaaaatatc tatgatactt ctaaaaaatt gcataaaaca gccaaaatat ggggaacaga
      541 tgattttggc ggctttggtt tcttaaattt tgtttttgaa ggactagtat ctggagataa
      601 gtatggatct gctgtaggga tcgctgcatt gatccttgtt gttggaggtg cttttggtat
      661 tattatgcgt accggagcga tcgacgcagg tatctatgcg tttatcagta aaactaaggg
      721 actggagcgt ttagctttac cgctattatt ctttgctttt tcattcggtg gcgctacatt
      781 cgggatggca gaagaggtaa ttccattctc aatggtgatg gttccatttg tcattgcgtt
      841 aggatatgac tcgatcgtag cagtaaccgt gacttatgtc gcatctcaag tcggaaatgc
      901 gacctcttgg atgagcccgt ttagcgtagc ggtggctcaa gggattgctg gaatccccgt
      961 gttgtctggt gcgacattcc gattgattat gtgggtagtt gttactgcat tagccgctgg
     1021 ttatatgatg atatatgcag aaaaaatccg taaaaaacca gaaagttcgt tgacttacaa
     1081 atcagatgat tatttccgtt ctcatatcaa aaagacatct gatgaaaata aaaaattcat
     1141 gcttgggcat aaattgatct tattagagat gttagttgtc cttgtctgga tcgtttgggg
     1201 cgtgactcaa aaaggctatt acattccaga aatcgcttct caattctttg tgatgggatt
     1261 agttgctgga atcattgctg ttcttttcaa attagatgga atgaaagtca atgacatcgc
     1321 ttcttctttc caatctggtg cagctgattt agctggtaca gcaatcgtag taggtatggc
     1381 caaagggatt cttcttgtct tgggaggatc tgatgcatct gttccttcag ctttgaatac
     1441 gattttgcat gggatcggga cggcgttgac cggtgttcca gctgtcattg gtgcttgggc
     1501 gatgtacatc ttccaaagtt tgttcaactt ggttgttaca tcaaattcag gacaagcagc
     1561 tttgactatg ccaatcatgg ctccactggc agatttagta ggtgtatcca gacaagtagc
     1621 agttcttgct tatcagcttg gttcaggttt tatggacgct tttacacccg tatcagccag
     1681 cttgattggt gtgctaggtg ttgcacggat cgaatgggca aaatgggcaa gattccaaat
     1741 caagatgcaa ggattcctgt ttgttttagg aactattttc atcatgattg ccattgcaat
     1801 aggtctgcag tagtactgga gacctgtaaa taaaaaagta atccaaacag tagataagga
     1861 ctccaagaac cgtcatttta cggtaaaatc aaaggagtaa agatcctatc gtttggatta
     1921 ttttttaaga tcctatcatc agttatactt aaagaaagag gaaaaatgta tgaaaatcat
     1981 tcgacaaatt gaagtgtttg caccagatta cttaggaaaa atggatgtac tgattgccgg
     2041 tgggaagatt ttggctgtag aagaacagct agaaggcggc tacgaaggag tggaagtaga
     2101 agaactttca ggtgagggaa aagtactaac cccgggtttc attgactgtc atttccatat
     2161 tttaggtgga ggaggagaag gcggttatca aaatcgaacg ccagaagtca cattgagcca
     2221 attgactaca gctggtgtaa cgacagttgt cggttgtttg ggaacagacg gagaaggtag
     2281 agatatgact gcgttgatca gcaaagcgaa aggtctggaa gcagaaggaa tctccactta
     2341 tgtatacgaa ggttcgtacc gattgccggt aaaaacagta acagattcta tcatcaaaga
     2401 ctttttgaca attgataaaa ttatcggaat cggagaaatc gcagtatccg atcaccgttc
     2461 ttctcagcca agttttgaag agttcgctcg tgcagtagca gatgctcgtg taggaggcat
     2521 gttatccgga aaagctggga tcatcaatgt ccacttaggc ggaggaaaaa gaaagctaga
     2581 gctattgaca cgaatcgtag aagaaacaga aattccgatc acacaatttt tgcctactca
     2641 tgcaaatcgc acaccagaat tatttgaagc ttgcgtggca tttgccaaac gtggtggcac
     2701 gattgacttc actgctagcg aagatcctga tttttgggag aagacagatg gtgaagttcg
     2761 cttcagcaaa gcactgaaac gattgatcga ggaagatgtt tctttagata atttcacgat
     2821 gacctcagat ggacaaggaa gcttgcctta ttttgatgaa aacaatcatt tcctagggtt
     2881 aggagtagga agtgccaagg cattgctagt agggatcaaa gaagctgtgc aaaaagaaag
     2941 tatccctttg gaaatcgcct tacgagctat aaccagtaat ccagcacgta tattgaaatt
     3001 ggataaaaaa ggaaaaatcg aaattggtgc tgatgcagat ctgtgtatct tggataaaga
     3061 aacgcttgat attgatacag ttatcgctaa aggagaaata atggtccaag aaaaagaagt
     3121 caaagtatgg gggactttcg aaaaaagttt ttaatcaaca gctctaacta tcttttttag
     3181 aaaacgagcg ttctgtttgt ctgtatctgt tgttgatgtt accctgaagg tagatcaata
     3241 aacaaactaa gaggaggaag cacaatggca gatttaaaag gacgttctaa agatatgaaa
     3301 gataaagtag tcggtgaagg aaaagaagca tacggcaaag caacagacga caagagcaaa
     3361 gaaacagaag ggaaagcaca atcactaggt gctgacatca aagaaaaagc tcgaaattta
     3421 ggcgacgaag tcaaagacaa gttttctcga aaagacgaaa aaaaataaaa accacttatt
     3481 actaattgaa tcaagcaaat aaaaaatccg aaccatatct gcgagacaga tatggttcgg
     3541 attttttatt tgatcattat cctgtttttt aaacttcgat tttttgaaat tttttatttt
     3601 ttagttcaaa gacttcatca cagctttttg ctacttcaat ctcatgagtc acgatgataa
     3661 tacagcgatt ttgttcatgg gcgatttctt ggaaaagttt gacgatatct tgtgaagttg
     3721 tttcatctag atttccagta ggctcatccg ctacgattag ttgatgttca cagcagatag
     3781 cacgaacgat agcgactcgc tgctgctgac ctcccgaaag tttggtcacc tgtttttttg
     3841 ctaatatttc atcaattcct acacgagcca gtcgatctaa agcaacttgt cgataattct
     3901 cttgcttggt cttagtgatt tccattgctg ctatgacgtt gtctagagcc gataaataag
     3961 gaagcaagtt gtaagcttgg aaaataatcg agacatcatt tttacgataa cttcttaacc
     4021 cgattttgtt cagtgaacga tcttgatagt atacctttcc ttcttttggt gcatctagtc
     4081 cagaaataag cgaaagaaaa gtcgtttttc cagaaccact tgctcctaaa atagcgtaca
     4141 tttttccagc ttcgaaattc agttctacat ctttataaag cgcatcttgc ggatcttgat
     4201 accaaaatcc caattgttct gtccgtaaca tcaaattcct cctttagttt aataggattt
     4261 ttcttgggtt taatcttaat atccctatag aagctaaaaa gacagaaaac agactgattc
     4321 caaatgctac tccggctaat agtcccaatt gtgcaggttt gacattgatt tccaattcgc
     4381 tgacttgatc agaaacagca aatggattat tcgtggttcc ttgttgacca tcgcgttgac
     4441 cgccttgttc ttgtctgtta cctggcattt gtccaccgcc aggagcttgc tggttttgtg
     4501 tcgtggtttc tgtctgttgg tcaagtagct gctgtcccac ggcattcgcg acgacgtttc
     4561 cgctaagcga agcaactcct aatgcaagaa tcatacaaat agccacttcg gtaaacaatt
     4621 ggagaatgat tttacttctg gattctccta aagatagcaa gacgcctaac tcatgccgac
     4681 gctcccgaat ggtaatcata atgatcagag taagtatgat aataccggct acagtcacta
     4741 gtaaaacaac atttttagaa aaacttgcca cgttgtttaa aggttccaac attgactgat
     4801 acatggaatc atttgattgt aagctaaatg tatctgtgtc gatcagcttc tcagcttctt
     4861 tgagaaactc atccatttta tctggatcag atagagtgta aatagcagaa tctatcgtgt
     4921 tatcgtcaga tgtcccattt agttcatttg caaaagtgta agaagtgtaa agtgtattcg
     4981 acggattcat gaagttgaat ttcataccca tactgccgct tgtttcgctg gattcataaa
     5041 ttcctttgat cgtcatttca taagtggtat cttcatcttc agtactcgtg attgtgaaag
     5101 tatctccgac tgccaggtca tttgtttctg ctagagtaga atcaatgact acattgtttg
     5161 tgcctttgtc gctgtcatct attccttcac catccgtgat ttttgctgtt ccatcagaaa
     5221 aattactatt ttgtgcagtt gctgacacac cactgatttg aaagtctgct tgagtcattt
     5281 gcggattccc gcccattcct ccagcaggtt caccgcctgc atcagtagtt tctgaactgt
     5341 cttcacttga atcagaacta gtgatggcag ttattccatc ttttgccata gctgaggcgg
     5401 aactttcaaa ggaataactc tttacattat ctaattcagc tattttttcc gcgtccgtta
     5461 aggaaactgg tgtaagagaa aaactgcctg gatctggacg ttcttcagtg gtagaaccgc
     5521 ttgaagaagt ttgttcctgt tttttaaatg aagcttctcg attggcactt aaagtcactg
     5581 ttgctccaac ttctttttgt gcttgattcg ctgcttcttc tgcagcgctt cgaatcgtga
     5641 ggccagctag cacaaaaata agaatggctg tgaacacggc aaacaataac aatgaacgtc
     5701 cccattttac ttttgtactt ttccatgcac gtttcataaa attcatattt tgcatccctc
     5761 ctagtagatg catttgataa tagacaacct atcttaaagc tagcttaaat cttatgctgt
     5821 ttgcacacca tcaacatgag gttctgtata tataaaaagt cactatagtt attgcaaaaa
     5881 atgtggtaaa atgaaaaaag tatgtttcaa accgacgaag aagaaatgaa agaggaatag
     5941 caaaagaaat cctagatgct acagagggat ttttacgtgc atagttgttc taacaattgc
     6001 tgatactagc gatttagttt atttgttgta taataatggg gaacagctga atacttatat
     6061 aagtattctg gctatagcgt ttcgcaaaag atccattttg ttaatgaagt atatccaaaa
     6121 taaaatgccc atccaactgt cagaacgcgg atgtgctggt aattttcctg ctgattctcc
     6181 ataagttgag aaatagtgat tttgcatatg cttcattgga atggctcata tatttagata
     6241 aagaagaaaa tcgacatctt taaacttgga tgtcataaga agaggtgaag tttgtgagta
     6301 ccattaaaat tatcccatta ggcggcgttc gtgaaaatgg aaaaaacatg tatgtcgcag
     6361 aagtgggcga ggatattttt attttagatt gtgggctaaa atatcctgaa aatgagttgt
     6421 tggggatcga cgtcgtaatc cctgatttta cgtatttaga agaaaacatt gatcgtgtag
     6481 ctggtgtctt tttaacacat ggacatgcag atgcgatcgg ggcacttcct tacttgttgt
     6541 caaaaatttc tgtaccagta ttcgggacag agttgacgat cgagttagca aaattgaatg
     6601 tcagtcgtaa tgaagcagcc aaaggattta aggatttcca tgtggtagat gagcatacag
     6661 aaatagactt tggtgaaaca gtagtgagct ttttccgtac cactcatacg ataccagatt
     6721 cagtggggat caatctgaaa acaaaagaag gtagtatcgt ttatacggga gattttaaat
     6781 ttgaccaaac ggctatccca atgtatcaaa cagattatgc acgattggcg gaaatcggca
     6841 aagaaggagt cttagcctta ctaagtgaat cggcaaatgc agaaaatcct cagcctgtag
     6901 cttctgaagc ccaaatagca gatgaagtat ttgatacgat caaatattgg gaagggcgta
     6961 tcatcgtcgc ttgtgtagca agtaatttgc aacgagtgca acaagtattg gatgcagcag
     7021 aaaaagcaca acgaaaagtt gtcttgactg gtcaagattt cgaacgaatt attcgcacag
     7081 cgatgcgttt agaaaaactt caattaccaa gtgaagatct gcttgttacg ttgaaagaca
     7141 tgaaaaacta tgatccagaa gaactgatca ttttagaaac tggccgaatg ggtgaaccca
     7201 tcaagtctct gcaaaaaatg gcaaacggta cacaccgtaa tgtcaagatt gaagaaggag
     7261 atttggttta tatcactact acgccaagca tcgcgatgga aaccatcgtg gcgaaaacgg
     7321 aagatatcat ttatcgagct ggtggaacag tgaagttgat ctctgagaat atgcgcgttt
     7381 ctggccatgc caatcctaat gacttgcaat taatgatcaa tttgatgaaa ccaacatatt
     7441 ttgtgccagt tcaaggagag tatcgtgaat tagctgctca tgcagatttg gctcatgcag
     7501 tagggatgcc atacaaaaat atctatatta ctggacgcgg agacgtgctg gaatataaga
     7561 acaagcgtat gagtgttgca ggaactacaa gtgcagaaaa tgtaatgatt gatggattag
     7621 gcataggtga tatcggaaac attgttttac gtgatcgaaa aatcttgtca gaagacggga
     7681 ttttcgtagc ggttgtcact atcagtcggc gagaaaagaa aatcatttca aaacctcaaa
     7741 ttacttcacg gggattcgtt tacgtaaaag ctagccgtga tctgatgaaa gaaagcagca
     7801 atatgattga aacaatcgtt gagaaacatt tagaaagcaa tgattttgag tggagcaaat
     7861 taaagcaaga catccgagat caattaggcc gttacttatt tgaacaaaca aaacgccgcc
     7921 cagtgatctt gccagtcatt atggaagcta ctcaacgtcg aaataaaaaa taaacaaaaa
     7981 gagtaagatg aacctctcta tagagtgctc gtcttgctct ttttttgcga aaaacaaata
     8041 atttttgaat acagcatccc tcgctttata ataaacgata gaataaaggg agtgaatcaa
     8101 tgatgagtca aacaaaagtc gaacgttatt tagaagaaca aaagatcagc tatcaaccac
     8161 ttgattttag tcatgtggta gaagaatctt tcacagaaga actacaaaaa agagggattg
     8221 atccagcttt gatatgtaaa actctcgtag tgaaaggaaa caaaactggg gtgatcatcg
     8281 cattgattcc tttgaacgat cacttagatt acaaaaaaac gagacaagtg acgaaagatc
     8341 gtaaagttgg ttttcctgga atggattttg tattaatgca tacaggatat ccccacggag
     8401 caaacacccc tattggaata aaactcgcac atcctgatta ttgtttttta gcagacagtt
     8461 ctttaaaaga aaaagaagaa gtaattgtgt caagcggtga aatcgggaga agccttcgga
     8521 taaaagtagc cgatttagaa aagatcgtcc atcctattta cgctgattta attagttaag
     8581 ggacgagaag agaagctaag cagagcagag acctcaaact tgagattata tctgcttggt
     8641 acttggtgca tcctgtatta tctgttacaa ttgctttgta catactgtac tatcaatcca
     8701 atgaaaatat atttagcagc tatatttttt cattgatgtg caagtgatga aatcgttttt
     8761 aataaaaaag atacagagaa aacgcgaggt gtaaaatatg aaaattacgt atcatggaca
     8821 ttcctgttta acaattgaaa tggaaaatgg ccaacgcgtg atgattgacc cgtttattac
     8881 aggaaatcca ttgacagact taactgcaga agaagtaaag acagattggt tgctgattac
     8941 tcatggtcat agtgaccaca taggagatat gattcctatt gcaaaagaaa atcaagccac
     9001 aattatcagt attgttgaga ttgctgatta tgcgaacagc agaggagtag attcattcgg
     9061 tatgaatatc ggtggaaaac atgcgtttcc attcggcgtg gtaaaattcg tacatgcaca
     9121 gcatagttca agctatgaag tagacggtat cgttcagtat atgggcgagc ctagcggaat
     9181 tattattcaa gcagaaggaa aaacgattta tcatgcaggg gatactgctt atttcagtga
     9241 tctaggattg cttgcagaag aattcgatat tgatgtcgct ttcttgccga ttggcgataa
     9301 ctatacgatg gggccagaag atgcagcaaa ggctgcgcaa acaattcgtg ccaagaaagt
     9361 agttccaatc cattacaata ctttccctgt cattgaacaa gatcccgaat cttttgtaga
     9421 gttactggaa gatagtgaag gaaaaataat gagcgtctcg gaaacgctgg aagtgtaggc
     9481 agaaaatggc gacaaaacat gatttgattt tagaatatat tgaaagttta cctgttggca
     9541 atcgaatttc tgtaagaagc atcgcaaaag aattgaatgt cagtgaagga acagcgtacc
     9601 gtgcaatcaa agatgcagaa aatgtcggac tagtttcaac aattcaacga gtagggacca
     9661 tccgtatcga acgcaaatta aaaaaacata ttgaacgttt gacatttggc gaggtcgtca
     9721 ggatcattga aggggacgtt ttaggcgggg cggcaggatt agacaaggtc ttgaataaat
     9781 tcgtcatcgg cgcgatgact aaacatgcca tggaacgcta tattacgcca ggatcactaa
     9841 tgatcgtcgg caaccgggcg gaagtccaaa aattagcatt ggaagatggc gccgcagtat
     9901 tgatcacagg aggattcaat acgactgctg agattgcaaa gctagctgat gatcttgaaa
     9961 tgccgatctt acgcacctct tatgatacat ttacagtcgc aaccatgatc aaccgagcgt
    10021 taagcgacca attgatcaaa aaagatatca tgcttgtcag tgatatctat atgccagctg
    10081 aaaagactca ttatttgcag cagacagata cgatttctga ttatcagcgt ttgtcagaag
    10141 aaacgcagca ttcgcgtttt ccagtcgtca accgccatca tcgtttgatg gggattgtta
    10201 cggcaaaaga tgttttaggg aaatcgccaa accagatcat cgatcgggtg atgacgaaag
    10261 aaccaatcag tgtaaagaaa acaatgagta ttgcctctgt tagccatcaa atgatctggg
    10321 atgggctaga agtcatgccg gtcgtttccg atgacttgac attggaaggc ttgattaccc
    10381 ggcaagatgt aatgaaagcg atgcagcttg tccagcgaca accgcagatt gcagatacga
    10441 tttcggatca gatatctgga aatatccata cgattgatac tgaccgtgaa ggaaatcgtt
    10501 tggaaaaccc taagttcaaa ttcgtagtag caccacaaat ggtcaatggc gttggaacca
    10561 tttcttttgg cgtacttagc gaaatcattt ctgatgtcgc acaaaaaaca atggtgatga
    10621 accaaaaacg aaatatcttg attgaacaag tgaacctaca ttatttgcgg ctgatccaat
    10681 tggaaagcga attagacatt cgtccacgtg tattggaaat cggcagacgt tccgctaaac
    10741 tggatatcga agtgtttatt gaaaacacca tcgtttccaa agcaatcgtt gtttgtcaag
    10801 taatggaacg ttcttagaaa gcaggagtga aatgacagta cagcaagaaa tagtagatac
    10861 aataaaaaaa tacgataaaa tcattatcca caggcatttg cgccctgatc ctgatgcatt
    10921 aggatcacaa gtaggattag ccgaactatt acgggaaagt ttcccagaaa aagaaatacg
    10981 tcaagtaggt gaaacgattg aagggcttcg ctttttagca gaaatgcaag aagttgaaga
    11041 tgtcttctac caaggagcgc tagtcatcgt gactgataca gctaattcac ccagaatcag
    11101 tgatcaaaga tatcagttgg cagaaaaact gatcaaaatc gaccatcatc ccaatgatga
    11161 cccttatgga gatcttgtgt gggtaaatac aaaagcaagt agctgtagtg agatgattgc
    11221 agaatttgct ttgatgtttc ctgaatcttt aaagatgaac gttaaggctg cacggttact
    11281 ttatgcagga atcgtcggag atacagggcg ctttttgtat ccctccacga cgtcccgtac
    11341 attagagatt gcttcgatat tgagaaatta tccatttgat gcaagtgctt tgaacagaga
    11401 gattgaacaa atgccgatga aagtcgctaa actttccggt tatctttatc aaaatatcca
    11461 ggtggatgaa aatggtgcag gaagaataat ccttccgcaa gaaattcttg ataaatatgg
    11521 aatcgatgat tcagagacag cttcgatcgt ttcacttcct ggagtgattg acgaagtgct
    11581 ggcttggggg atttttgttc agcagccttc agggtattac cgcgtacgtt tacgttctaa
    11641 ggggccgatc atcaatgaat tggctaaacg ccatcatgga ggaggccatc cattagcaag
    11701 tggtgcgaat gcaaaagatt tagcagaagt agaggaaatc tataaagaga tccaagatat
    11761 atgccaatcg tataagagca gaaaaacaga atgatacttc gttcgaaaac gtgccttttg
    11821 tagtaaaaat cacaaatgta tcttttcaag atcttctttt tccgttacaa tagtgctcac
    11881 taaagatttt aaaggagaaa gaagatgaca ttaccaaatt gcccagaatg tggctcagag
    11941 tacacatatg aagatcgagg cttgttgatt tgcccagaat gcgggaacga atggaacaaa
    12001 gaagaacaag aaactgtcat agaagacgga ttagtggtat tagatgcaaa tggaaaccgt
    12061 ctgttagagg gcgatagcgt gacagtgatt aaagacttga aagtaaaagg tgcgtcaggt
    12121 gccattaaac aaggaacaaa agtcaagaac atccgtttag tagaaggcga tcataatatc
    12181 gattgcaagg tagaaggatt tggaccgatg aaactgaaat cggaatttgt gaaaaagaat
    12241 taaagaatat tcaaggtctt ttcacattct tttcctacta ctaatgataa aataaagtaa
    12301 acgaaaagga gtgaaacgaa aatgtatggt tatggaatgt atggtttttt agatccgaca
    12361 tttcttttgg tcattcttgg attagttatt tccggaattg cgtcagcgta cgtcaatagt
    12421 actttccgaa aatacgatca agtgagaagc aaaaacaatg tgactggtac acaagcagca
    12481 caatatatat tacaaagtca acagataaat aatgtcggtg ttcaacaaat tgccggagat
    12541 ctcacagata actacaattc aggtaataag atgttgagtt tgtctcaagc aactgcacag
    12601 tctacttcag ttgctgcgat aggtgtagca gctcatgaat gcggacatgc ggtacaagat
    12661 gcttcgggtt atgtcccatt gaagattcgt gcagcacttg ttccagcagc taactttggt
    12721 tcgatgattt cgttcccctt gattatggta ggtgtattgc ttagctggaa ctctacgtta
    12781 atcaatatcg ggattttcgc tttttcttta gcattacttt tccaattggt gacattacca
    12841 gtcgaattta acgcgtctcg gcgagcaatc caaattttga gtgaaggcgg actactgacg
    12901 gaagaagaag taccgatggc aagacacgta ttatttgcag cagcactgac ttatgttgca
    12961 gcagcgcttt cgacattcct gcagctgttg cgcttgatct tattgttcgg cggcaaccga
    13021 agagattaaa ataaagaagt tgtgaaatca gcgctttgat ctgagctaat accgagagtt
    13081 agctgattga ataccattta tttggaata
//
LOCUS       DACDIG010000071        12738 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.071, whole genome shotgun sequence.
ACCESSION   DACDIG010000071 DACDIG010000000
VERSION     DACDIG010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.071"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IV247_002240"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IV247_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP8778298.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="IV247_002241"
     CDS             complement(322..891)
                     /locus_tag="IV247_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAP8778299.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="IV247_002242"
     CDS             1077..1979
                     /locus_tag="IV247_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8778300.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IV247_002243"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IV247_002243"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAP8778301.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IV247_002244"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IV247_002244"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAP8778302.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IV247_002245"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IV247_002245"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAP8778303.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="IV247_002246"
     CDS             8547..8732
                     /locus_tag="IV247_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAP8778304.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="IV247_002247"
     CDS             complement(8794..9990)
                     /locus_tag="IV247_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP8778305.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="IV247_002248"
     CDS             complement(10160..11596)
                     /locus_tag="IV247_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP8778306.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="IV247_002249"
     CDS             11889..12698
                     /locus_tag="IV247_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8778307.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2673 c         2250 g         4012 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gacaattt
//
LOCUS       DACDIG010000072        12633 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.072, whole genome shotgun sequence.
ACCESSION   DACDIG010000072 DACDIG010000000
VERSION     DACDIG010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.072"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..682)
                     /locus_tag="IV247_002250"
     CDS             complement(<1..682)
                     /locus_tag="IV247_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISEfa7 family transposase"
                     /protein_id="HAP8778308.1"
                     /translation="MQGVIAMMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFE
                     FIYPIVEATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYS
                     FEEKIPPFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANA
                     NKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPE
                     SGYYVKGER"
     gene            1316..2287
                     /locus_tag="IV247_002251"
     CDS             1316..2287
                     /locus_tag="IV247_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAP8778309.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            complement(2365..2601)
                     /locus_tag="IV247_002252"
     CDS             complement(2365..2601)
                     /locus_tag="IV247_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8778310.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(2570..2701)
                     /locus_tag="IV247_002253"
     CDS             complement(2570..2701)
                     /locus_tag="IV247_002253"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8778311.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            complement(2777..3160)
                     /locus_tag="IV247_002254"
     CDS             complement(2777..3160)
                     /locus_tag="IV247_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP8778312.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            complement(3193..3978)
                     /locus_tag="IV247_002255"
     CDS             complement(3193..3978)
                     /locus_tag="IV247_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HAP8778313.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            4156..5256
                     /locus_tag="IV247_002256"
     CDS             4156..5256
                     /locus_tag="IV247_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8778314.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            5246..6070
                     /locus_tag="IV247_002257"
     CDS             5246..6070
                     /locus_tag="IV247_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase"
                     /protein_id="HAP8778315.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            6076..6882
                     /locus_tag="IV247_002258"
     CDS             6076..6882
                     /locus_tag="IV247_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8778316.1"
                     /translation="MKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLANYS
                     DIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLAANF
                     AGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYPSMK
                     EVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALTSSN
                     VNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            6879..7709
                     /locus_tag="IV247_002259"
     CDS             6879..7709
                     /locus_tag="IV247_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8778317.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            7716..8747
                     /locus_tag="IV247_002260"
     CDS             7716..8747
                     /locus_tag="IV247_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8778318.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(8854..10127)
                     /locus_tag="IV247_002261"
                     /pseudo
     CDS             complement(8854..10127)
                     /locus_tag="IV247_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            10404..10613
                     /locus_tag="IV247_002262"
     CDS             10404..10613
                     /locus_tag="IV247_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778319.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            10663..11379
                     /locus_tag="IV247_002263"
     CDS             10663..11379
                     /locus_tag="IV247_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAP8778320.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(11281..11801)
                     /locus_tag="IV247_002264"
                     /pseudo
     CDS             complement(11281..11801)
                     /locus_tag="IV247_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            12089..>12633
                     /locus_tag="IV247_002265"
     CDS             12089..>12633
                     /locus_tag="IV247_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8778321.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEET"
BASE COUNT         3627 a         2353 c         2850 g         3803 t
ORIGIN      
        1 ctcgttcgcc tttgacgtag tagccgcttt ccggatcagc tttacttatt tttctttctt
       61 tcagcttcac ttccttttct aagtccaaag taaaagggcg ttttccagcc tctattcgtt
      121 gacgattgat ctcatcttct aattcatcct gataggcttt cgcttcatca tgggtcattt
      181 ctttcgtaaa cttatgttta ttggcgttag ctttgatgtg ggtagaatcc agatacaaat
      241 tatcctctgt tacaaaacct gctttaactg cttgttctaa aatataggca aaaatatcct
      301 caaacaccgt cgtttctcta aatcggcgaa cataattctt tccaaaagtt gagaaagggg
      361 ggattttctc ctcgaaggag tagcctaaaa accagcggta agcgacattg gtatcgactt
      421 ccttaatggt ttgtcgcatg gaacggatac cgaataaata ctgaataaac accaatttga
      481 tcagaatcac tggatcgata cttgggcgac ctaaatcgga gtaagtggct tcgacgattg
      541 gataaataaa ttcaaaatca agcgcggcat caatctttcg aacgagatga tcttgaggaa
      601 ctagatcatc aatcgtaagc atcgcaaatt gatttctacc attcgtattg tttttagaca
      661 tcatagctat cactccttgc attcgttttc ataaatctat tataaaagaa aagactgtag
      721 acatcatcca taaaatggac tttgtctaca gtctggataa cgtgattttt tgctgtaatg
      781 acaaggtttt agctgtttca gataccatgt atccaaaaat agtttattga gaaaatagga
      841 gagtatttga tactaactag taggaaaaaa tgagataaaa agtaaatgga atcatttaag
      901 gtaatctgaa ataaaaaata tacagccctg tggtacttta cctacaaggc tgtatatatt
      961 ttttactctc agcgtttttg actccgtgtc tgactccgtt ttattgattt tgaatgttat
     1021 tcaatgacca tttaacaggt caaatttgat gtttttgatt ccgtatgacg ccgtttgata
     1081 tctagtgata tagtgatttg agtctacatc gaataaggga ataaaccaat aaaaaatgat
     1141 ttagaatatt aaaataacaa aaaagaggac tacctgtatt agtcctcttt tttgaccaga
     1201 ttttgaccag tctattttac atcctagtat atttatatga gctaatttta tgattaaatg
     1261 gtaagttttt ccttgtgtgt tttagtttaa actagtgtgt aattcaaaaa tctatatgga
     1321 aggaacagta ttaagaatag aacagggttc tctacatgat ggtgctggtt tacggaccgt
     1381 tgtttattta aagggctgcc cattacgctg tgcctggtgt tcgattcccg aatcacaaag
     1441 caagcagatt gaaaaaggat ttggccagac catgaccgct gaagaggtaa tggatgagat
     1501 tgagaaggat gctgtttttt actttcactc ggatggcgga gtgaccatta gcggaggcga
     1561 ggcactggtt caggcggatt ttgctaaaga aattttgcaa aaatcgaaat acattggaat
     1621 caataccgta ctggaaacca gcttttgtgg tgcttataat gaaatccaaa aggtagctcc
     1681 ctatgtggat acattgtttg tggatgtaaa aatgtttacc agcaagctcc acaaacaatg
     1741 gaccggctta gataaccaac aaattttgaa gaatattcgt cgctttttaa ttgagtatcc
     1801 taactgcgaa gtgcgaattc gagttcctgt ggttccaggt atcaatatga acctgaccga
     1861 gctgctgacg attgcttgtt ttgtggcaga tcttgatcgg ttcgtaccgt tagaattact
     1921 accgtaccac tgctatggta tgcacggtta ccaagcattg ggactggagt atccgttggc
     1981 agacacgcca gcgccttcac ctaaagaaat gtttgcattg gcagatcaac tggctcgcac
     2041 ggttcctcat ttgctggtca cagctttaac caaaacattt atttatttat tgaatccaag
     2101 ctttatctta tggataaatg aaagacactc actagctgca gccagctcaa gaatagagct
     2161 tttgctgaag tcagtgagtg tctttttacg atgttttttc gaagttaatt tgtcgaatga
     2221 acactggctc aatcggtaca tcttcactgt ttgtcggctg agctgcgatc gctgctacga
     2281 tttctaaccc ttggtatacc tgtccaaaaa cagtataacg accatccagc tccggtagtc
     2341 ctccaagctc caagtatgct tgctttacat tagcaggtca gcctgcgcct ttgtgttttg
     2401 cacaataaaa aactggttgc cgttggtgtg ggggccgtgg tcccctaagg ccaaggcgcc
     2461 attaaaatga taaagtccgc gatgcacttc atcagcgaaa aagccctcat agctggaaac
     2521 agcttcatca ccagaaccat ccaaagcgcc accttgaata acaaattcct taatgacacg
     2581 agccgaaagg tgtgttgtca taaacgcctt gctcagctag ggagcgccag ttggctacag
     2641 ccaggggagc ttggtcagga aataagcgga gacgaatttc accagcatcg gtgaccagca
     2701 cacaaacttc ttcctgctgc ggtttttcta attgaaagag aggaaggttt gcaactgaaa
     2761 cgccttgatc ctgccatcaa atatcgaaaa tccgccactc gaccttcgcc tgatcaccaa
     2821 ggcgagccat tcccaataaa acggtatagg cggtttcatc cgcttgaaag gtcaattgca
     2881 gctgtcctcg taaatcgttg tagtcatctg gggcggctac ttgataaaaa agatgaaact
     2941 gcgcatgcaa atattccaaa ggctgatctt taaaggctgc aaaataagcc tgaacttttt
     3001 cccgctgcaa gctgtcagcc accaaaaaat tttccagttg ttgttttgga acggctgcta
     3061 acaaggatcg gcaaaccgcc tcaattttca acgcatccca atgtcgcgca attaaataat
     3121 ggatgggtgc ctcttgattg tagtcccaca ggttgctcat ttttttcacc actttcttct
     3181 attatatagg aatcacaaaa tcggcagcgg cgtcaggcgg cgttcttttc cttgaatgac
     3241 tagcaacttt tctacctgac agtctgccaa cagctgactc atttttttaa agcccagctg
     3301 ataatcttcg gcaacatgag catccgagcc taaggtaaag agctttccgc cgacactttg
     3361 atacaaagga acggcataac ggtaaaggtc ttcgttgccg tatttcaaga agctcttggc
     3421 atttaactcc atcgccaatt ctcgttcaac aacctttttg aaaatcgtta gcagctgagc
     3481 ctcaaaatgc tccgcgaatt cttccgctgt gaaggtgaag cggcgtaatc cataatcaaa
     3541 gtgagtcaaa atatctcctt ctggaaaatc cgtcaagacc tgcaacatct gatcaaaata
     3601 ttccttggta gtttgaatgg gatctttggt taaaaccaca tcgtccatat aatcaaagcg
     3661 gccattttga tgaatactta ccagctttaa atcataagga tgctgagcga ggtaatccac
     3721 gatccgctct tcttgtcctg gaacaacgcc aatctcgata cctcgcagaa atttggtgga
     3781 ataggtttct tttaaagagt ccagtttgtt tatataggct ggataatccg gaatgtcatc
     3841 ttttccggta gatggattgt ttaaatcaaa gtgatccgta gaaacgaaaa actccggctg
     3901 ataggctaaa tagttttcaa atttttcctc tgaatcaaag gaaaaatacg tgtggagatg
     3961 ctgatcataa tactgcatag ggagcctcct tttttctact atcataccgc aaaagcttta
     4021 gaaggttaac gggaattcgt cgaaatttac aaaccatata caacctttac attgttcatg
     4081 aaaccttttc aagaaactga ttctctcatt acaaagcttt gttagaatgg aattatcgaa
     4141 aggggagaag aatgaatgaa aatgaatgaa aaaattcgta acatgactat cggtttattt
     4201 tgtgcagcaa cagtaagtat cctgctgaca ggctgcggct caggtgaagc aacgaagcaa
     4261 tcggctgcgg cagacgactc tttagatacg attatagaaa aagccaagga agaaggagag
     4321 attgccagct tgggaatgcc ggatacttgg gcaaattggg taggcacttg gaacgacctt
     4381 gaaaaagaat acggcttaag tcacgtggat acggatatgt ccagtgcaga agaattggct
     4441 aagtttgaat cagaaggaaa aaatggaacc gccgatattg gtgatgtcgg tatcagcttc
     4501 ggtccgttgg ctgaaagcaa agagttaacc ttaccttata agaccaacta ttgggaggaa
     4561 attccggatt gggcaaagga tgatgatggg gattggttgt taagctatac gggaaccatt
     4621 tcctttatca ccgataaaga gaatgtgaag caagcaccga ctagctggaa ggagctgcaa
     4681 gagggtgact atcaagttag cgtgggggat gtgacgattg ccaaccaagc acaatttgct
     4741 gtattggctg cggcgattgc caacggaggt gacgaaaagg atattcagcc aggtctgaat
     4801 tactacaaaa aaatggcaga aaaaggccgt ttgtccaccg ttgatgccag cgtagcaaat
     4861 cttgaaaaag gtgaaatcga tgtggcagtt gtttgggact tcaatggact aaattatcgt
     4921 gaccaaattg actccgagcg ttttgaggta actgtgccaa cagacggctc catcattagc
     4981 gggtatacaa ccttaatcaa taaaaatgcc cctcatccca atgcggcaaa gctagcacgg
     5041 gaatatattt tatctgatga agggcaaatc aatttggcaa agggctatgc gcgacctatt
     5101 cgcaaggtag agctgcctaa agaagtagct gaaaaattac tgccggagga tgcttatgaa
     5161 gcggcggtgc cagttgaaga caatgaagct tggaacaaga cctctgtaga gcttccagag
     5221 ctttggcagg cggaggtact gacctatgca aagtagactt gacaaactga ttgtagttat
     5281 actagacggc tgtcgctacg acacagccgt cgcccagctt gggttcatga atcatttagt
     5341 tgagcaccag caagcaagct tattgaaggt aaaaagtgag ctgcccagca attcacgtcc
     5401 gttatacgaa gtactgatga ccggcgttcc agcctatgaa aatggcatct atagtaatta
     5461 ccatgctcag cgatccaaag agtggtcgct gtttgaatta gttaaggaag ctggcggaaa
     5521 aacaggagcc gccgcttacc actggtacag cgagttgtac aatcaagccc cctataatcg
     5581 tttggttgat cgcatccagc atcaggagga gcgactgatt cagcatggca tcttttatag
     5641 tgaggacact taccctgatt cgcatttatt tgcggatgcc cattatttag tgaatcagta
     5701 tcagccagac tttttggtcg tacattcgat gaatattgat gatgtcggtc acaaatttac
     5761 cgccgattct catgaatatg cagcagcggt taatcaggcg gacagattat tggctattta
     5821 ccttcctgaa tggctgcaaa aaggttatca agtcgtggtg actgcggatc acgggatgga
     5881 tgcgcacgga ctgcatggcg gcagcttggc tgcacatcgt gaagttcctt tttatttgat
     5941 ttcaaataaa cgccaaaagc tgaaggaaca aactgtacca cagctgctgg cggctccatt
     6001 gttttgctgg ttattaggaa ttgcaccgtc agagaaaatg caggacattc aagaattgat
     6061 gaggggatga aagagatgaa aaaacgcttg gttctggttc ctgcctcctt tatcttctgt
     6121 gcatttttac tgctgccgct gctttttatg gttctgtgca gcttgcacag tgatttaact
     6181 aaccagtgga cattggccaa ttacagtgat attttcagca acaaatatta cacacaggcc
     6241 tttgttaaca gcgtactgat tgctgtcagc tccagtgtga ttgggttaat cgggacgttt
     6301 attttatgtt tatgcttaat gaatctatcg actaaattgc aggagaaaat cacctttgtt
     6361 tccaatttgg cagctaattt tgctgggatt ccgctggcat tctcttttat tattttgttg
     6421 gggaatgtag gtgtgctgaa aatgattttg ccatttctag cggacttcaa tttgtattct
     6481 tggtggggat tgaccatcac gtatacctat ttccaaattc cgttaggggt cttgtttttg
     6541 tatcccagca tgaaggaagt gaaaaaggaa tggctggagt ccgcacaatt gctgggcgct
     6601 tcatcagcgt atgcttggtt caaggtggct ttgccttttt tgcgaccatc gattgccagc
     6661 acctttgtca tcttatttgc taatggcatg gggacctatg aaacagcgta tgccctgacc
     6721 agcagtaatg taaatctttt aacgattcga attgcggctt tagtgtcggg agatgtgttc
     6781 gccaagccta atttaggcag tgcgttggca gtcgctttag gtgtcctgct ggtaacggca
     6841 atgttgatta ttcaaaagaa aggcaaggtg gtgcagacgt gaaaaagtta ataaaaccga
     6901 ttttgttctg ctttgtcctg tattacttgc tgccgattat tggaacgatt ttgtatgcca
     6961 gctccactaa atggtcaaaa tcgcttttac ctagtgattt taccttgcaa tggttccaac
     7021 agctgctgac ggacagggaa tttattgctg cggtgggtcg ctcactttta ttagcaggtg
     7081 tggtcttggt gacgattctg ttgttgatga ttcccaccat tatttggatt catttgtatt
     7141 tccctcgttt gaatcgttgg ctggaaaagc tgctcctgct gccgtatgct ttaccaggag
     7201 tgattttggt aacggctttg cttcgaacgt atgctgaaac tggtatccca atgtttgtag
     7261 tgttggtagg tgcattgttt attacggcgt tgccgattgt ctatcttagt cttaacaacc
     7321 agatgcgctt aattaatctg aaggaattag tggatgcagc agaaaccttg ggcgccccga
     7381 tgtctacgat tattatccag gtgctgtttc caaacatccg tatcggtgtg accctagttt
     7441 ctctgatgat tttttcttcg gtttttgggg agtacatgct gacgaacctg ttgattggcg
     7501 gtcgatttca gacggtacgc atctatatgc tgcggcggat gaacgaaaac ggccatttgg
     7561 ctagcgctgt gatggttttg tatttgctgt ttatggtggg aattgcttta actaccttta
     7621 tgctgactta tcgacaaaag caacagctgt cccttaagca gacgagggaa gagaagcagt
     7681 cgaaaaagaa gcccttaaag gagttgtgac ggaaaatgtt tttagaaata catgatctca
     7741 atctgttttt tgatgaaaaa cagattttag ctattgatga aattcagatt gaaaagggtg
     7801 agctggttac tttactaggt cccagcgggt gcggaaagtc tacgttgctg cgctgtatca
     7861 cgggattgga acaaccaaag agcggtgaga ttgtgctgga caatgagaat attgccgata
     7921 agcctacgaa ggatcgcaat atcggctttg tttttcaaca gtatgcattg tttccaacta
     7981 tgactgtttt tgaaaatgta gcctttggtt tgaaggtaaa gaagctgagt cctgaggtga
     8041 ttcaggaaaa ggtttttgag atgctatcat tggtggatat gacggagcag gcagataaaa
     8101 atgtccagtt tctttctggc ggtcaacgac aacgggtggc actggctcgc tcattggtca
     8161 ctgaaccgaa agtcctgctg ctggatgagc cattaagtgc tttggatgcc cgaattagaa
     8221 aacagctgca gcgggatttg cgcgctatcc agcaatcgct agggatgacg atgatttttg
     8281 tgactcatga ccaagaagag gcgatgcgaa tcagtgatcg gatttttgtg atggaagctg
     8341 ggcgggtggc tcaggtttcc acctcgaagg aattgtatca gaatcctcag agccgatttg
     8401 ttgcggaatt tatcggcaac tataatcgtt tcgaatggta tgagctgaat cagtataccc
     8461 acgagttttc cgctaagggc aatcgctgca tttattattt aagaccggag ctgattcaat
     8521 ttgaaccggt tcctcagggg gttaagattc cggttctatg gaaggaatca tttattttag
     8581 gcaacattca acggtatgtt tttcaaacgc tggagggcaa agaaatttta gtggatcggt
     8641 tgaacgatcg ggaagagaaa attagcgatg ccttatacat tcagctgaaa gatattctag
     8701 caatccctgt ggaaattgat actcagcatt ttgcacaaca aagctagtgt atagtcggca
     8761 aacatttctg gctctttgtc aaataggact gatagagtga tataagaaca tctaaggtag
     8821 aattgatttt ctgctttaga tgttttttta tgtttaataa gtgatcagat gattgattaa
     8881 gaaaattctg gttctcatcc taacccgtaa gctattcgtt tcaatgcttt gatgtgggta
     8941 tgcagatttt ctattttgcc atttgagtaa ggcaactcta aggctaaacg aatgccggat
     9001 tcatagttta taaacgcttt ttttatatga tgcagttctc ttggaaccga cttgggcatt
     9061 gtttttacaa gctgaaagaa gatgtccgct tctttgtcat aaaaagcttg aagaatttct
     9121 tgatagtaag cataagctat tcgtagttca tcagaagtag aaagtagacg ttctacgact
     9181 tcactttcgg ttaaataggc ccaaccaaaa ttacgccatt ttgtccattt atcataaaca
     9241 agcgtgtcct gtcgtttgat cagaagtcga gataaagcct tcacttgtcg atagtgtttg
     9301 gcttgtattg gatcgtttcg gtcgaagctt ttcatgactc atagtcaaat atccttaaaa
     9361 ctgcgaatga ggtgcttgac gatgtgaaag cgatccacaa cgagtttggc atgaggaaag
     9421 cactctttta tcagcaaagc ataagaggcg ttcatatctg ttacgagtat ttttacgttt
     9481 aaacgtgcac gtcgttcaaa ttgcataaaa taagaaatga gatctttctt cttacgtgaa
     9541 ggaagaatat ccagtaattt cccagtatcg ccatccatac aagaaaaaga atagggaccg
     9601 acttgatttt tcaatgaacg aaattcatct accaatgaac agcgaggcaa ccaatttatt
     9661 ttcacgtttg tcatggggag cagagaagcg agtgttcgcg aaacggttga ccaagaaacg
     9721 ccgcaatgtt tagaaatcgt cgtcattgct tgtttttcag tcaattcctc caatatttta
     9781 taatgtactt gtttggcgat aaaacagttt ttttcaacaa tgtaggtatt cgccgtaaaa
     9841 taggtgtgac agccacgaca gaggtaccgt tgctttgcca aagcaagata agtccgttga
     9901 ttcgcacact tattgagaag gaccagcgat tttttctttc cgttcttaac gatcagccca
     9961 tgttgattca cctgtttaca ctgaggacag ggctgaatcg ttttaatgag cgtggcagaa
    10021 acaacgatac tgttgattga atgtattttt ttcttctcga catgagtgat ttgaatgttt
    10081 ccatctttga ttcctagtaa tagtttgata agatgaagat caggcataca gaacgctcct
    10141 ttgattgtgt tgtggtgatt caattctaac ggagcttctg tatgttttgt ttttttatgc
    10201 tcaaaaggac tgatagaatg atttactatc agtcctagaa attatagcgc cacatttctt
    10261 tcacctttga cttttcagac ggcagttttt atgctgatac tgtagagaga gcaggacaca
    10321 gcttttgctt gttttgggta agggggaaag aagatgacca aagcacttat ttttgatttt
    10381 gatgggacga tgattgacag tgaatggatg ttttacgaga gtattcatca ctatttactg
    10441 aagcctattt actggagcag ttcggctata aaattccttt tgcagactat caacaaggtg
    10501 ttgggacggc agcagaactg tccttttatg aagggataag tcaagcggtt gggagaccag
    10561 tggatgctga acattttgag cagcggatca gcagcactgg aaaatcggag tagtctcaat
    10621 tctgaactgc gggagctaga ctattttttt gaagcacatc cgatgattaa ggaaaaaatt
    10681 gattcctttg tgacgatcga tgatcttcag gtcggcaaac cagatccaga aggttatttg
    10741 ttgtgcttga aacggctgag ggttgaacag gctgttgtag ttgaggatag tccagtcggt
    10801 gcccaagcag caccgcatat gacagttgat gtacaagcgt tggatgcaga ttttttcgcc
    10861 tttagcgggc ataaaatgtg cgggccgaca ggaatcggtg tattatatgg caaacgtcaa
    10921 ttgttagaac aaatggagcc agtcgaattc ggcggtgaaa tgatagattt tgtcaatctt
    10981 tacgatagtt catggaaaga gctgccatgg aaatttgaag ctggtactcc gaacattgca
    11041 ggagcaatcg attacttgaa tgagattggt atggagaata ttcatcgtta tgagcaagaa
    11101 ctggttgcct acgttttacc taaattacaa gcgatcggcg gtatcaccat ttacggaccg
    11161 caagatcctg aacatcatac tggcgtggtt gcttttaatt tggatggcat ccatccccgt
    11221 gatgtggcga ctgctctgga tatggaaggt gtcgctgtac gttcaggaca ccattgtgca
    11281 caacggttca tcgacgattt ctgtttgact aattggtcgt ttaaacagtc gatgataatg
    11341 ccgtttgagc ggctccaatg tcgtggaatc tttgagtaag agtttccaat atcgcttgag
    11401 ccgacggtac ttcttctgat tttcagatta cgtgattgat tttcgtacag ttctatgaga
    11461 atcgcctgct tcagttcctt tgttagttga tgatgttcat caactaagtc cgttttcgca
    11521 gaaaaggtct gcccacaatc atagcataga aaacgggtac gttttaattc taaaatagtc
    11581 aacttttctt tgactttcaa catctgagta cgtgtcgtat aggaaccgtt gcggataata
    11641 cgaatcgatc cacagtgttc acaatgggaa ggtgagtcat ccaatctccc tttaataaac
    11701 tgcgcttgaa cgcctttata ttctttttca atcagccatt cttcatcaaa aaataaatgt
    11761 ttatctgtta atccgagtaa ttttctagta tgattatcca tagagcgact cctttggtag
    11821 ggtgatgttt tggtcgacta aattttacca aaagtgagtc tctttttgag ttgaaacgaa
    11881 gaaaaagtgt tagtagaaaa tcgccttcga ttttccaaca acacaaaata ttatagaagc
    11941 cttttttcta aaggatttca aggtgtctgt ttgcatttat taaaaaaaag aaaaagtatt
    12001 ggcaacagaa ggcgtcacca acactagata ttatagagcc ataatatgct gtataatatc
    12061 tagtgtatag gcttaagtgg aggtatatat gaaaatatta actgttgaag atgataatat
    12121 gataagagag ggaataagtg aatatctttc agaatttgga tatactgtta ttcaagctaa
    12181 agatggaaga gaagccttgt caaaatttaa tagcgatata aatttggtta tcttagacat
    12241 tcagatacct tttataaatg gtttagaagt gttgaaagaa attagaaaga aaagcaattt
    12301 accaattcta atcttgactg catttagtga tgaagaatat aaaattgatg catttactaa
    12361 tttagtagac ggatatgtag aaaagccatt ttcattgccg gtcttaaaag ctagaataga
    12421 ttctttaatt aagaagaatt atggacattt agagaagttt gaatataagg atctatcggt
    12481 taattttaat agctatacag ctaaaataaa tggtgaagaa atagatgtaa atgcaaaaga
    12541 actagaagta ttaaaatgtt tattggataa taatggacaa gtgttaacaa gaatgcaaat
    12601 tattgattat gtatggaaag atagcgaaga aac
//
LOCUS       DACDIG010000073        12590 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.073, whole genome shotgun sequence.
ACCESSION   DACDIG010000073 DACDIG010000000
VERSION     DACDIG010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.073"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            47..899
                     /locus_tag="IV247_002266"
                     /pseudo
     CDS             47..899
                     /locus_tag="IV247_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            996..2882
                     /locus_tag="IV247_002267"
     CDS             996..2882
                     /locus_tag="IV247_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8778322.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAADGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFETPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMSLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVINYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(2923..3825)
                     /locus_tag="IV247_002268"
     CDS             complement(2923..3825)
                     /locus_tag="IV247_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8778323.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            4055..5458
                     /locus_tag="IV247_002269"
     CDS             4055..5458
                     /locus_tag="IV247_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP8778324.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            5458..6891
                     /gene="ascB"
                     /locus_tag="IV247_002270"
     CDS             5458..6891
                     /gene="ascB"
                     /locus_tag="IV247_002270"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP8778325.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            7036..7962
                     /gene="trxB"
                     /locus_tag="IV247_002271"
     CDS             7036..7962
                     /gene="trxB"
                     /locus_tag="IV247_002271"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAP8778326.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            8176..9903
                     /locus_tag="IV247_002272"
     CDS             8176..9903
                     /locus_tag="IV247_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAP8778327.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIQKMTEE"
     gene            9976..10269
                     /locus_tag="IV247_002273"
     CDS             9976..10269
                     /locus_tag="IV247_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP8778328.1"
                     /translation="MPDTQKTKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(10364..11224)
                     /locus_tag="IV247_002274"
     CDS             complement(10364..11224)
                     /locus_tag="IV247_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAP8778329.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            11316..11744
                     /locus_tag="IV247_002275"
     CDS             11316..11744
                     /locus_tag="IV247_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP8778330.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            11906..12082
                     /locus_tag="IV247_002276"
     CDS             11906..12082
                     /locus_tag="IV247_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAP8778331.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            12109..12555
                     /locus_tag="IV247_002277"
     CDS             12109..12555
                     /locus_tag="IV247_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAP8778332.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         4119 a         2170 c         2775 g         3526 t
ORIGIN      
        1 tttttgtttt ggatacagcc ctatatatca tggaaaggag caaaacatgc tgattgagaa
       61 agtactgaat aacaacgtcg taataaccag ggatgaaaga aatcaggaaa tgatcgtgat
      121 gggaaaaggt ttggcattca aaaagaaagt gggtgacccg atttttgact catgcatcga
      181 caaaaaattt cagttgacaa atgacgggct ttcacaacag ttccaagaat tggcacaagc
      241 gattcctctt gagtacttag aaatttcttg tgaaatcatt gaatatacaa aagaggctct
      301 acaagtccag ttgaatgatt ctgtgtatat tgcattgacg gatcatttgt atacggctat
      361 cgaacgtcac aaaatgggaa tatttgtacc aaatgtattg ctgtatgata tcaaacaact
      421 atttcctaaa gagtatgcca tcgggaaaaa aaacagtgaa gaaaatctcg gataaatacc
      481 acatcaagtt atcagaagat gaagccggat ttatcgcatt gcatttagtc aatgcacaag
      541 gcattcaaag ccaacaagac aatacctacc tgttcactat aacaatacag gatattctaa
      601 atatcgtccg ctattactat aagattacat ttgatgaaga ttcagtccat ttttttcgat
      661 tcgtcacaca cttacgtttt tttctgacac gcgtcctaga tggagaaaaa tcagaagtgg
      721 gagaagtttc tgaggaattg tttcaggtta ttcaaaaaaa atataaggat acgtttggtt
      781 gcgttgagaa aatagcagac tttttagccg agacgttcaa ctaccagatg tccacggacg
      841 aaaaaatgta tttaacgatc catactgcgc gtttagtgca tgagaatcgt tgtaaataaa
      901 taaaaaaagg aggataaatt aacaagaacg tatgaaataa gggtagtgac attcagttgg
      961 ctaaaccttc cactaataaa ttggagggaa tagaaatggg aaaataccaa gaattagcga
     1021 gaaaaatcgt agaaaatgtc ggcgggaagg aaaatatcaa tggtttgaca cattgtatca
     1081 caaggcttcg tttcaaatta aaaaacgaag aaaaagcaaa tgatgagatc ttgaaaaata
     1141 tggacggtat tgtaacggta atgcgagcgg gagggcagta tcaagttgtc atcgggaatc
     1201 atgtgccagt cgtgtttgaa gaggttataa aagcaggaaa ccttactttt gatgaggctg
     1261 tttcaacaga aaaaatgaga ccgtttgata tgcttattga tggaatatcg ggctgtttcc
     1321 aaccattcct tgctatccta gctgctgacg ggatgattcg tggactgaca gcattccttg
     1381 tcttcttagg cgcgtttgac cgcggttctg gcacatttgt tatgtttgat aatatcggtg
     1441 attctgtatt ccaatttatg cctgttatta tcggtttaac agctgcccgt aaatttaaag
     1501 taaatgaatt cgtagggatg ttgcttggcg cagcattaat gaatcctagc ttgtcgctgg
     1561 aagctttgag tggtgcagca gaagcaccac taacgaccat attttctggc actatttttg
     1621 aaacacctat ttatcaaaca gtattcggca ttccctggat tgcgcgtaac tacgcttctt
     1681 ctgtcattcc cattatcttt attgtgttgt tagctagcca aattcagaaa ccaattaaaa
     1741 agttagtacc tgagatgatc gcgaatttct tcgttccatt cttcaccgtt cttattacaa
     1801 tgcctttagg ttttttactc gttggaccag tattcacatt cgcaactgat attttaatgg
     1861 ctggatttga aacactttta gcactttcgc cagttattta tggagcaatc gtaggcttct
     1921 tctggcaaat tctggtgatg tttggtctcc attgggcaat cgtaccaatg ggattgatgc
     1981 aattttctgt gaatggttgg caaaatatta tgacacctgt tgcagtagtg tcttttggac
     2041 aaactgcagc acttaccgca ctctacttta agcttagaaa tccaaaagac aaagcaattg
     2101 caataccagc cattgtttct ggtattgtgg ggattacaga gccagctatt tatgggttta
     2161 cattgccacg taaaaaaata tttattttta cctgtgtcgg tggtgcgtta gggggcgcgt
     2221 atagtgggtt aatgaattta acttcttgga atcaaggtgg actaggcatc tttacaattc
     2281 ccaactatat tagaccagat ggtgatttaa cagatgtgat taatgtattg attgggatcg
     2341 ccattgcgat ggtggtatcc tttgcgctga cttttttctt ttggaaggag gaagcagatg
     2401 aaacagatga tagacagaaa agatcaggta aagaaattgt aaaaacaccg atacaaggac
     2461 aaatcgctcc actgaatgca gcaaaggatg cagcttttgc acaaggaaca ctaggtcgag
     2521 gaatcttgat ctaccctgaa aaaggagaag tacgtgcccc ttttgacgga acagtcatgt
     2581 ccctgtttcc gacgaaacat gcaatcggga tggtttctga agcaggtctg gaattgctga
     2641 ttcatgtcgg attggatact gttcagttgg aagggaaata ttttgaaagc ttggtgcagc
     2701 aaggagaaag agtgaaacaa ggcgatctgt tgcttcgctt cgacaaaaag aaaatcgaag
     2761 aggaaggata cagtttagaa acacctgtga ttgtcatcaa ttacatggat tatttggata
     2821 ttttggaaaa tcaaggggaa acggtcgggc cagacgatgc acttatcacc gcactgacct
     2881 gaacggttag gtgaaagaat ggactaggct taactattct ttttagtcca cttctttatt
     2941 ttcaaaattg aaaggctctt tcatgatggc tgagctgata gggcgtttat caaatttttg
     3001 tcctacagaa attagatgga ctaaattctg ttgataattt tctgcaaaaa cacaactata
     3061 taacacgtct agaagataac aaatcgatgt attgatcgta aaattaccaa ttttcgaata
     3121 cagtcgttcg cgtgtcgtaa gtcgcaaagc ggctgtactc attgaagata gcgttgaatc
     3181 accgatactg gtcaaagcta aaattggcac atgattttgt ttcaatattt ctgcggtacg
     3241 aaggatcaca ttgttttcac ccgtataaga aatcagaatc ccacacatat cacttgtgca
     3301 attgtacgct tcatagaacg attcaccaaa agtggtgaca atcgtgacat ccattttgat
     3361 gcgcttcatt ttcagcatga aatcttgaga aatcaaagta ttcgagttac tggcgaaaat
     3421 cacgattttt tttgcttgaa gcattagttg tttggctttt tgcagttggt catgatgaat
     3481 gagagaaagt gtatcttgaa tcgtcatctg ttctaaagaa gccattttat ttgcgatcgt
     3541 cataataccg tctgtggtgg aaaaaggaag attcgcatct acgtcttgaa aattcccttc
     3601 aagataggct gtttcagcta aaaaagcatt acgaaaactg acccagccat caaaacccat
     3661 tttttttgct acacggatga gtgtggacgg gtggacataa catcgctcag caatctcttg
     3721 gatggtcatt tcgccaagtg ctggagaatc gcttaagaga aactgaatca cttcattttc
     3781 tgaaggagaa aaggagattt ctttgatttt ttcacgtaat aacatgtaga taccttcttc
     3841 ttgttttttt acgtaaatta taactttttt gaaaatgatt tgtaaacgga tttatgttat
     3901 atagcttaat aaagacattt tttgacaaga tatttacaaa aatccagaag aaaacactaa
     3961 cttttgtaaa tgttactgga aagaaacgaa tttctattgt ttgttttgtt tacaaaaaag
     4021 ataataaaag tgtaaaaagt aaaggagtga gaacatggca aaaggaatca aaatcgctac
     4081 gattggtgga ggcagcagtt acacccccga gttgatggaa ggatttatca aaagatatga
     4141 ggaattaccg atacgagaaa tctggctcgt agatgtagaa gaaggaaaag aaaagcttga
     4201 gatcgtcagt cagatgtctc agcgtatgtg ggatgcttct ccgtatgaag tcaaaatcta
     4261 tgcaactctg gatcgggaag aagcattgaa agatgcggat tttgttacga ctcagtttcg
     4321 tgtcggtcaa ttagatgcac gaatcaaaga cgagcggatc cctagttatt atgggatgat
     4381 tgggcaagaa acaaatggtg ctggcgggat attcaaagct tttcgtaccg ttccagtggt
     4441 tttggcaatc gttgaagaca tgaagcgttt atgtccaaat gcttggctga ttaatttcgc
     4501 gaacccaagc gggatggtga cagaagcagt tatcagatat ggcaaatggg ataaagtgat
     4561 cggattatgc aatgtgccag taatggcgat gatgacagaa ccaacaatgc ttggcaagaa
     4621 aaaagaagag ctgatctaca agtttgccgg tctaaatcac ttccattggc acaaagtagc
     4681 agataataaa ggaaatgacc gcacggcaga attgattgat cgtttatacg atgaagacaa
     4741 tggattgccg aaaaatatct ttgacatccc gtttttcaaa gaacaattaa aacaaatgaa
     4801 catgatccct tgtggctacc accgctatta ctaccgtgaa gaagaaatgc tggaacatgc
     4861 tttagaagaa tatcaaacga ttggtacaag agcgcagcaa gtgaaagaaa cagaagcaga
     4921 gctatttgaa ttatacaaag atccagaatt agaccataaa cctgaacaat tgcaaaaacg
     4981 aggtggcgct tattattcag acgctgcctg tgaaacgatt gcttcaatct atggaaataa
     5041 agaaacacag atcgtggtat ctacaaagaa tgatggagct gttcctgacc tgccggcaga
     5101 ttgtgtagta gaagtcactg cctatgttgg cgcacaagga gcgagaaatg tagcgtttgg
     5161 ttctcttcct gctgctgaaa gaggctggtt gcaagtaatg aagaatatgg aattgctgac
     5221 gatcgaagca gctgtgactg gtgattatgg gacagcttta caggcgttta cgatcaatcc
     5281 gcttatacga tcaggaaaga cagcaaaacg gatcatggat gagttgttta ttgcacacca
     5341 gcagtattta ccgcaattca aggaaacaat cagccggtta aaagcagaag gaatcgaagt
     5401 ccaagatgaa ctggcaagag aattatcaag caatacacat gtaatggagg aagtatgatg
     5461 ggattcagaa aagatttttt atggggcgga gctacagcag ccaatcaatg cgagggcggt
     5521 tatgatgaag gcggacgtgg gttagcgaac gtagacgttg tgccaatcgg agaagaccgt
     5581 tttcctgtca ttactggaaa aaagaaaatg tttgaatttg atgaaaatca tttttatcca
     5641 gcaaaaattg cgatcgatat gtatcatcat tataaggaag atattgcttt atttgcagaa
     5701 atggggttca agacttatcg gatgtccatt gcttggagtc gtatttttcc aatgggcgat
     5761 gaaaaagagc caaatgaatc tgggttgaaa ttttatgagg atgtctttaa agaatgtcaa
     5821 aagtacggga tcgagccgct tgtgacgatt acacattttg attgtccgat gcatttgatc
     5881 aaagaatatg gtgcgtggcg ttccagagaa ctcgttggtt tttatgaaaa tctgtgccga
     5941 gtcatcttca atcgctataa aggacttgtc aaatattggt tgacattcaa tgagatcaat
     6001 atgatccttc atgcaccatt catgggcgca gggctgtatt ttgaagaagg agaaaatgaa
     6061 gaacaaatca aatatcaagc ggcacatcat gagttggttg caagtgccat cgcaacgaag
     6121 attgctcatg aagtagatcc ggagaacaaa gtaggctgta tgctggcagc aggagcatat
     6181 tatccataca cttgcaatcc tgaagatgtt tgggcttcta gaaaatcaga tcgagaaaat
     6241 tatttcttca ttgatgtcca gtcacgagga gaatatcccg cttatgcatt gaaagaaatg
     6301 gaacgaaatg gcattcagat cgaaatggaa cctggggatg ccgcattgct gaaagaacat
     6361 actgttgatt ttatctcctt ttcttattat tcttctcgag tttcgactac tgacaacgaa
     6421 ctcttggaac agactgcagg aaatatcttt gcttctgtca aaaatccata tctggaagca
     6481 agtgaatggg gttggcaaat tgatccatta ggcttgagaa taacgatgaa tgatatttat
     6541 gatcgttatc aaaaaccact atttattgta gaaaacggct taggtgcagt cgatacgccc
     6601 gatgagaatg gttatgtgga agatgattat cgaatcgact atctggcagc acatatccaa
     6661 gcaatgaaag atgcagtaga attagatgga gtcgatctat taggctatac gacttgggga
     6721 tgtattgatt tagtatcagc tggtacaggt gagatgaaga aaaggtacgg attcatttat
     6781 gtcgatcgtg acaacgaggg aaatggcaca ttagcccgtt cgaagaagaa atcattcaac
     6841 tggtataaaa aagtaattga ttcaaatgga gaaaatcttt cgaatgattg atcaatgatt
     6901 tataaaagtc tattgttttt cagcggattt ttcgagtata ataaagctat tcgagaaacg
     6961 caagcagaac aatagacttt ttttcgcatt ggctgtaaag tatgcttact tgattaagta
     7021 aaggagaatc tacatatgta tgatgtaatc gttattggcg caggtcctgc tgggatgact
     7081 gctgctttat atgcttcacg ttcaaactta tccgtattga tgatcgaaca aggcgcccct
     7141 ggcggccaaa tgaacaatac ggcagaaatc gaaaactatc ctggtttcga ttcaatcatg
     7201 ggtcctgatc tagcttataa gatgtatgaa ggtgtttcaa gatttgggac agaaaatgct
     7261 tatggaatcg tgcaagatat caaggatcac ggcagccaca aagaagttat ttgtgaagat
     7321 aaaacttacg aaggaaaaac agtcattatc gcaacaggct gtatccaccg taaattaggc
     7381 gtacctggcg aggaagaatt tgctggacgt ggcgtttctt actgtgcagt ttgtgatggt
     7441 gcgtttttcc gtaacaaacg tctagtagtg atcggaggag gagactcagc agtagaagaa
     7501 gccatctatt tgacacaatt tgcttctgaa gtagtcatcg tccaccgtag agatgaatta
     7561 cgtgcacaaa agatcatcca agaccgtgca tttgcgaatg aaaaaatttc ctttatgtgg
     7621 gatacagtcg ttgacgaaat cgttgggaat gaaatggtag tgacaggtgt gaaagcacgt
     7681 aatgtcaaaa ccggcaatga gtttgatttg gcagcagacg gagtattcat ctatgttgga
     7741 ctggatccat taactgcacc atttagagaa gcagggttga cgaacgaagc tggctggatt
     7801 ccaacagaca acgaaatgaa gacgaatata ccaggcgttt ttgccatcgg agatgttcgt
     7861 gaaaaagatt tgcgacaaat cactactgct gttggtgatg gtgggatcgc tggacaacaa
     7921 gtgtataaat acttggaatc tttagaagaa acaactgcat aagtttaaaa agagtggaag
     7981 aacggtttca attgttcttt tactcttttt tttcgcgtga aatctactga atccgattta
     8041 tgcgaatact tgcaaccgtg cttgataaaa gaatcctttc ttgatttaag cgttttttgg
     8101 tcctcatact tttatatcta ataagtgaat gctatactta ttaagaacca tatttcagaa
     8161 agaggtgtta ttttcatgtc ttgggaacaa gtgtatgaac aatgggtaca tgaagaaaat
     8221 ttagaagaaa acttaaaaaa acaattaaat gatctgggtg aagatcctga aaaacttgaa
     8281 gatgcttttt atgcaccgtt ggaattcggt actgcaggca tgcggggaat cttagggcct
     8341 gggatcaatc gaatgaacat ctatacggta agacaagcaa cagaaggact tgcacgtttc
     8401 atggatacac aaggcgaaga aacaaaacgc cgaggtgtcg ctatcgctta tgactcacgc
     8461 catcaatcac cagaatttgc catggaagca gccaaaacat tagcacatca caatatccca
     8521 tcttatgtat tcgaaagtct acgtccaaca ccagagcttt cttttgcagt ccgtcatttg
     8581 aagacgtttg cgggaatcat ggtaactgca tcccacaatc cagcagctta caacggatat
     8641 aaagtctacg gcgaagatgg gggacaaatg cctcctgcag acgcagatgc attgacgaaa
     8701 tatgtccgtg aagtcagcaa tccgttaaaa gttgaagtat tatctgacga agaagctgaa
     8761 catagcggat tgatcacgat catcggagaa gaagtggatg ccgcttatct agaagaaatc
     8821 aaagcagtaa cgattgatca tgaattggtg gaaacaatgg ggaaagattt gaaacttgtg
     8881 tacacaccac ttcacggaac aggaaaaatg ttgggcgaac gtgcgttgaa acaagctgga
     8941 ttcgaacaat tcgtactagt tccagaacaa gctgtcgcag acccagattt cactacagtc
     9001 aaatcaccaa atccagaaga acactctgct tttgagtatg cgatacgact tggcgagaaa
     9061 gaaggcgctg atttgctgat tgcaacagat cctgatgcag accgtttagg tgcagccgtt
     9121 cgtttgccaa atggctccta tgaagtattg accggaaacc aaatcggagc actgtttgct
     9181 caatatattt tggaagcaca ccaatcgcga ggcactttac ctgaaaacgc agcggtattg
     9241 aaatcgatcg tttctagcga attacctaca gcaattgcgg aacactatgg ggctaccatg
     9301 ttcaatgtat tgacaggatt caaattcatt gctgaaaaaa ttcaacaatt tgaagaagat
     9361 cattcccata cttttatgtt cggattcgaa gaaagttacg gttatttagt taaacctttc
     9421 gttcgcgaca aagacgcgat ccaagcattg ctcttattag cagaagtagc agcttactat
     9481 aaaaaacaag gcaaaacgtt gtatgacggc ttgcaaacga tttttgaaac ctatggctac
     9541 tacgcagaaa aaacgatttc tgtgacaatg agcggacaag aaggttctgc gaaaatctct
     9601 gctttgatgg aaaaattccg tgaagaagca ccaacagaat ttgccggagc aaaagtcatc
     9661 caaacagaag acttcaaaga gttgacgaga acagaccgtg aaggaaacgt agaaaaacta
     9721 acgacaccgc catctgatgt attgaagtat acgctggaag aaaatggctg gatcgctatc
     9781 cgtccttcag gaactgagcc gaaaatcaaa ttttacatcg gtgtaaaagg aaactcttca
     9841 gaagacgcag ctgaaaaagt agccgcttat gaagcgatta tccaaaagat gacagaagaa
     9901 taaaaaaact tgagactgat ggaaaaagtg gtaaacttta gaagtcagtc ttttttcaag
     9961 ttggaggtga atggattgcc agacacgcaa aaaacaaaag tagagaaagt cagccaacta
    10021 ttcaaaatgt tgagtgaccc cactcgtctc aaaattctgc tgtatctgaa agacggggaa
    10081 caaaatgtga cagcaatcac ccaagcagtg gaaatggaac aatctgctgt atcgcatcaa
    10141 ctgcgtttat taagagaaaa ccatgtcgtc aagtcccatc gtgagggaaa agcgattttg
    10201 tatagtcttg atgattctca cgtactggat attttgaacc agacactcaa acatgtggaa
    10261 catcaataac gataggtgca aatagagaca aactggactg tgtcaacgaa tcttcttcgc
    10321 tgacacagtc cagttttttt taagaaaaag acaagaaaaa ctgttagtct gtttcttcgg
    10381 tggtttcagt tccataataa ctatgatctt ctaggtctaa agcattcaaa ataagcgaag
    10441 ccgtttcttc aatagataaa gatgccacat tgatcactac acatcctagt ttttcataaa
    10501 gatcattcgc aaaatcgagt tcagctctaa ttttttcgat atcggaataa gcagtatcag
    10561 ggtttaatcc gtaagagcgc atccgttctt tacggatatt atttaaaata tctgggttat
    10621 tcgttagtcc cacgattttt ttaggatcga tttcccacag ctgtttggga atatgtgctt
    10681 ggggaatcaa aggtaaatta gcaactttta aatttttatt tgctaaaaac aagctcaaag
    10741 gcgttttgga agttcgggat acgcctagca aaacgacatc tgcttctaaa aaaccacgag
    10801 gatctttccc atcatcgtat tttacagcaa attccattgc tttgatccgt ttgaaatagt
    10861 tctcattcaa taaatggagg gcaccaggtt gccgaattgg cttgatacca gttaaacgtt
    10921 caatttctga tatcggtggc gtcatcacat cgaaactaaa taattgatgt tcttcacaaa
    10981 aagatcgtgt tatctcgatc agttcttgat tgattaatgt atgcaggaca agtgcttttt
    11041 cttctaatgc atcttgcaat gcctgcctta atgttttttc atctgtcacg aacgttctgc
    11101 ggaataggtt gaactcaacg gtaggatact gagccatagt agcttgcgct agttttgtcg
    11161 cagtttctcc agcagagtcg gagatgacaa aaaaagtaac gatttctgta gaatcggaag
    11221 acatggataa aacctcactt gcattttttt tatgtttatt ataccatact ctagaatgcg
    11281 tacttcgtaa tcattttgat ttggaggaat aagtcatgaa aaaacagaca ttgatcgaac
    11341 aatcattaga aacattgaaa aataacggtt ttaaatatac taaaaaaaga gaagcattgt
    11401 taacttattt agtgaagcgt aaccgctacg tatctgctaa agaagttttt gactatatga
    11461 atcaggaatt caaaggggta agttatgata cgatttatcg gaatctccat gattttgcag
    11521 agatcgattt actggaagaa accgatttaa atggagaaat gaagtttcgt tttcattgct
    11581 gtcaggggga aatcggccac caccatcatt ttatctgtac agtgtgcgga acaacgaaag
    11641 aaatccatat gtgtccgatg aattatttcg aagaacagct aggtggatgc acgatcgaag
    11701 ggcatagatt cgagattttc ggaagatgtg aaaattgtca ataaggattt aaataaaata
    11761 acatttgata atttaaaaaa aaagcaagat ttccttgacg gtcaaaacag cattagatat
    11821 aatatttaca ggacagtctt gttttttgag atatatttca ataacaatta cggttacttt
    11881 ttaagctcgg agggagggaa ttaacatgtc aaaaacagtc gttcgcaaaa acgaatcact
    11941 tgacgatgct cttcgtcgct tcaaacgttc cgtgtcaaaa gcgggtactt tacaagaatc
    12001 tcgtaaacgt gaattctacg aaaaaccaag tgtaagacgc aagaaaaaat ctgaagcagc
    12061 tagaaaacgc aaaaaattct agttctgtga tgaattggag ttggtagtgt gtcactacta
    12121 agtagattaa acgatgacat gaaagcagcg atgaaagcga aagataagga atctttacaa
    12181 gtcatccgaa tgatcaagtc atctatacaa aacgagcaga ttaaagaagg tcacgacttg
    12241 acagaagaag aagaactaac ggtgttgtct cgcgaaatga aacagcgacg tgattctttg
    12301 catgagtttg aagaagcagg acgtgacgat ttagctgaaa aagtaaaaag tgaaattgta
    12361 atagtcgaaa aatatatgcc ggaacaactt tccgacgaag aaattcgaca acttgttcaa
    12421 gaagcaatca ctcaaactgg tgcttcatca atgaaagaat ttggcaaagt tatgggtgca
    12481 attatgccta aagtcaaagg aaaagcagat ggaaaccaag tgaatgcgat tgtgaaagaa
    12541 ctattacaat cttaatttta tcatttaaac tgcggacagt gattgtccgc
//
LOCUS       DACDIG010000074        12526 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.074, whole genome shotgun sequence.
ACCESSION   DACDIG010000074 DACDIG010000000
VERSION     DACDIG010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.074"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..109
                     /locus_tag="IV247_002278"
     CDS             <1..109
                     /locus_tag="IV247_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8778333.1"
                     /translation="YIPIKDIILHFEKYDYRFHWNSDNSVIEIWIPIQD"
     gene            237..1451
                     /locus_tag="IV247_002279"
     CDS             237..1451
                     /locus_tag="IV247_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP8778334.1"
                     /translation="MNDKNFIEELRQKREEYGVTQTRLAVACGISREYYNRIEKGKQP
                     LNDELREIIEKQIERFNPQEPLFLLIDYFRVRFPTTDALAIIRDVLQLKSNYMLYEDY
                     GKYGYESKYVLGDINIMCSMQEHLGVLLELKGKGCRQMECYLLAQERSWYDFMLDCMT
                     AGGVMKRLDLAINDKAGILDIPKLKEKYKAGECMSYFRMQKDYSGTEKCGSDLPKNTG
                     ETLYLGSTSSELYMCAYQKNYEQYVKNGTEIEDTEIKNRFEIRMKNERAYYAVVDLLT
                     YRDAERTAFSIINHYVRFVDREDDKPKSQWITNDDWAWFVGENREPIRLTTKPEPYTL
                     QKALHWLQRQVAPTIKMVQALDRENRTTILKDMIEQAELKDKHKHLLQLEKSTIEERI
                     DTAVPQENDGIF"
     gene            complement(2161..2349)
                     /locus_tag="IV247_002280"
     CDS             complement(2161..2349)
                     /locus_tag="IV247_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778335.1"
                     /translation="MAVREYHYLTVQVMAIKEDKSFTRTQIGFRSLLQGKGRGVFVST
                     ASCLSNVLNSFIQLSKND"
     gene            2440..2649
                     /locus_tag="IV247_002281"
     CDS             2440..2649
                     /locus_tag="IV247_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HAP8778336.1"
                     /translation="MNCLERFYRKGVCVIDNAVKTIQKKNGKCGIGNRGKTKIVVKEH
                     FSEKGKTMEELLTDVMLEKAKQTIA"
     gene            2761..4374
                     /locus_tag="IV247_002282"
     CDS             2761..4374
                     /locus_tag="IV247_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4368 domain-containing protein"
                     /protein_id="HAP8778337.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFDRPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIIEKIFSLAYQGYGSAKITKQLRAEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     GTNKTNKTPYSYYACSYYGQFGKGNCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASTLKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITSLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEDNERIQEIEIYYRFIGRVE"
     gene            4409..5620
                     /locus_tag="IV247_002283"
                     /pseudo
     CDS             4409..5620
                     /locus_tag="IV247_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            5643..6569
                     /gene="glsA"
                     /locus_tag="IV247_002284"
     CDS             5643..6569
                     /gene="glsA"
                     /locus_tag="IV247_002284"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAP8778338.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            6777..8237
                     /locus_tag="IV247_002285"
     CDS             6777..8237
                     /locus_tag="IV247_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778339.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIREIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            8458..10221
                     /locus_tag="IV247_002286"
     CDS             8458..10221
                     /locus_tag="IV247_002286"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAP8778340.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            10305..10763
                     /locus_tag="IV247_002287"
     CDS             10305..10763
                     /locus_tag="IV247_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAP8778341.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            10872..11555
                     /locus_tag="IV247_002288"
     CDS             10872..11555
                     /locus_tag="IV247_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP8778342.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            11702..12316
                     /locus_tag="IV247_002289"
     CDS             11702..12316
                     /locus_tag="IV247_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAP8778343.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            12332..>12526
                     /locus_tag="IV247_002290"
     CDS             12332..>12526
                     /locus_tag="IV247_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778344.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNY"
BASE COUNT         4329 a         2017 c         2637 g         3543 t
ORIGIN      
        1 gtatattcct ataaaagata ttatcctaca ctttgaaaaa tacgattatc gttttcattg
       61 gaatagtgat aattcagtta ttgaaatttg gatacctatt caagattaga agcaatttta
      121 tatccataca cacaaagcag ttagtcttag gattgactgc tttttctata cagattttta
      181 atgacaggca attatcaatt atggtagttg ccttttttga ttggaggaat tgaagaatga
      241 atgataaaaa ctttattgaa gaactaagac aaaagcgtga ggaatacgga gtaacacaaa
      301 caaggcttgc tgttgcctgt ggtatcagcc gtgaatatta caaccgcatt gaaaaaggaa
      361 aacagccatt gaatgacgaa ttaagagaaa tcatagaaaa acagattgag cgtttcaatc
      421 cgcaagaacc actattctta ttgattgatt actttcgtgt ccgctttcct actacggacg
      481 cattggcgat tatccgtgat gtattacaac tgaaatctaa ttacatgctt tatgaagatt
      541 atggaaaata cggatatgaa agcaaatatg tacttggcga catcaatatc atgtgttcca
      601 tgcaggagca tttaggtgtt ttattggaac tgaaaggcaa aggctgtcgg caaatggaat
      661 gttatctgct ggcacaggaa cgctcatggt atgacttcat gctggattgt atgacggctg
      721 gtggtgtgat gaaacggctt gatttggcta tcaatgataa agcaggaata ttggatattc
      781 caaagttaaa ggaaaaatac aaggctggcg aatgtatgtc ctacttccgt atgcagaaag
      841 attacagcgg tacagaaaaa tgcggtagcg atttaccaaa gaatacagga gaaaccttat
      901 atctcggttc aacaagtagc gaattatata tgtgtgctta tcagaaaaat tatgagcagt
      961 atgtcaagaa tggcacagaa attgaagata cggagattaa gaaccgtttt gaaatacgca
     1021 tgaagaacga acgagcctat tatgcggttg tagatttact gacctatcgg gacgctgaac
     1081 gcaccgcttt ttctatcatc aatcattatg tccgctttgt tgacagagag gacgacaagc
     1141 caaaaagtca atggataaca aatgatgatt gggcatggtt tgtaggggaa aacagagagc
     1201 cgatacgctt aaccacaaag ccagagcctt acactttaca aaaagcgttg cattggctac
     1261 aaagacaagt cgcaccaacc ataaaaatgg tacaggcatt agacagagaa aatcgtacaa
     1321 cgatactgaa agatatgatt gagcaggcag aactaaaaga caagcataaa catttattac
     1381 aattagagaa atcaaccata gaagaacgta tagataccgc tgttccacaa gagaatgacg
     1441 gtatttttta atgtaattga aaaatttcag atttagtcag caaagagctg ttttcattcc
     1501 ctatgtgaag tgaaagaaga aaaataaatt ttttattaaa tctgcaacaa aatggctact
     1561 tgcgtacaga tatggtgcga gaaaaggaaa tctaaacttt ttttgaaaat aggtcattaa
     1621 atcggggctt gctgtcccta tatgaagtga aagaagaaaa aagttttgaa aaaatgacgg
     1681 aaattcgtga ttagtggaca gtcttctgtg aaaagaggaa aaacatcatc attttgtatt
     1741 caaacaggca ttagcagttg agtgttgatg tgaaaagatg aaaatacttt gttctcaact
     1801 taacaattta aggtttccgt tccctatatg gtgcaaagaa aatatttcat tctattgttg
     1861 gcagtaacgg tatggtttgg gtagttaggg tgtgaaaagg aaaatattct tttttcatca
     1921 tcaacttagc aaaatgacat tttctatccc tatatggtgc atgaaaagaa gttaaaattt
     1981 ttccatttaa acttagcaaa tggacgtttg ctgtacagat agtagtgaaa aaggagaaaa
     2041 tcaaccgttg attgccaaaa tgaaccgttc catttgtact aataatggaa aaagttttgg
     2101 attttggtta cgtttctcct cttttccaac gcgatatata ggaaagacga tttttttctt
     2161 tcaatcgttc tttgacaact gaataaagct attcaatacg tttgacagac agctagccgt
     2221 tgaaacaaat acgccacgac ctttcccctg caagagcgag cgaaaaccta tttgtgttct
     2281 tgtaaaagat ttgtcctctt ttatcgccat gacctgtact gtcagataat gatactcccg
     2341 tacagccaca gcttgagcat taagagcgtc gcacgcaatg ggtacggcta cataagaact
     2401 atgcagaggt ggaactcctg tgggctatga caaaagccgt tgaattgctt agaaagattt
     2461 tacagaaagg gggtatgtgt tattgataat gctgtaaaaa caattcaaaa gaaaaatggc
     2521 aagtgtggca ttggcaacag aggaaagaca aagattgtag taaaagagca tttttccgaa
     2581 aagggcaaaa caatggaaga acttctaact gatgtcatgc tggaaaaagc aaagcagaca
     2641 atcgcataaa atcggtgcag ttgtaagaaa tagattgaat gacaaaaagt acccgtgtta
     2701 tactttactt gcaagcaggt attgtaaaca cgggttagtt ataaaggagg ataactgaca
     2761 atgaaacaac agatttacaa tactgcactt tatcttaggt taagccgaga tgatgaatta
     2821 cagggcgaaa gttccagcat taccacacag agaagtatgt tgcgtttata tgcaaaagaa
     2881 catcatttga atgtcattga tgaatatatt gatgacggct ggtcgggaac aaattttgac
     2941 agaccgagtt ttcaaagaat gattgaggat atagaggcag gaaaaatcaa ctgtgttgta
     3001 acgaaagacc tttcccgtct tggcagaaat tatattatga caggacaata tacagaattg
     3061 tatttcccta gtcataatgt ccgctacata gcgattgatg acggtgtaga cagcgaaaaa
     3121 ggcgaaagtg agattgcacc atttaagaac atcatcaatg aatgggtggc aagagataca
     3181 agccgtaaag tcaaatcagc atttaagaca aagtttgcgg agggggctca ttacggtgct
     3241 tatgctccgt taggatataa gaaacaccct gacatcaaag gaaaactgtt ggttgatgat
     3301 gaaacaaaat ggattattga aaaaatcttt tccctagcct atcaaggtta cggaagtgca
     3361 aaaatcacaa agcagttaag agcagaaaaa gttccgacag cgtcatggct aaattttaca
     3421 agatacggta cttttgcaca tatctttgag ggaaaacccg aaagtaagcg ttatgagtgg
     3481 acgattgctc atgtcaaggc gatattgaaa agtgaggttt atatcggaaa cagtgttcac
     3541 aatatgcagt ctacggtatc gttcaagagc aaaaagaaag tgcgtaagcc cgaaagtgaa
     3601 tggtttcgag tagaaaacac tcacgagccg attattgaca aggaagtgtt ctatcgtgtg
     3661 caggagcaga taaaatcaag acgcagacag acaaaggaaa aggcaacgcc gatttttgca
     3721 gggcttgtca agtgtgcgga ttgtggttgg tctatgagat ttggaacgaa taagacaaat
     3781 aaaacgcctt acagttatta tgcttgcagt tactacgggc agtttggcaa aggtaattgt
     3841 tctatgcact acattcgtta tgatgtgctg tatcaagccg tattggaacg cttgcagtat
     3901 tgggctaagg cagtacagca ggacgaagaa aaggtattga acaagataca gaaagctggc
     3961 aatgcagaga gaatacggga aaagaagaaa aaggcaagta cattgaagaa agccgagaac
     4021 agacaaaatg agattgaccg tttatttgcg aaaatgtatg aagatagagc ctgtgagaag
     4081 ataacagagc gaaattttgt gatgttgtcc agcaagtacc aaaaagaaca gatagaactg
     4141 gaacagcaga taacaagcct aagagaagaa ctaagtaaaa tggaacagga tatgataggt
     4201 gctgaaaagt ggattgagtt aatcaaggaa tattccgtac caaaggaact gacagcacca
     4261 ttattaaatg ccatgataga aaaaatcctc atacatgaag caacaacgaa tgaggataac
     4321 gaaagaatac aggaaattga gatatattac cgatttattg gaagagttga gtaatcaaca
     4381 agagtaatat ttttaactaa gggaaaccgg cggtgtctat acttgggtca gtcgcacatt
     4441 gggaaaacga ttcggttttg ccgcaatctt tttccaatgg tttgagataa cagttggcta
     4501 cttgaccatg ctgtatttct taactggtgc attatcctat gcaacgggta ttggtgctat
     4561 acaaaacaat aaatatctga aattggcaat cctattgatt attttctggg ctattttact
     4621 ctcacagtta aaaggaacca aatacacttc attgattgct cggatcggtt ttgtagctgg
     4681 tatcttgttg ccagcattag tcttgtttgg cttagggatc cattatgtcg cttcaggcgc
     4741 tccactacaa acgatactgt ctatgaaaac attgattcct gatttttcga aattgcctac
     4801 gctggttgtt tttgtttcct ttattttagc atatatgggc gtagaaacat ctgccagtca
     4861 tgcaaatgaa atggaaaacc ctaaaaagga ttatccatta gcaatgtttg tattggttat
     4921 tctggcaatc attttagata cttttggcgg attgaccgtc gctactacag tacctcaaca
     4981 tggtttgtct cttaatacag gggtgattca atcgttagaa ttcctattaa gacacattga
     5041 tccaaatctt gcatggattg cgaaaattct tgcgattctt gtttgtttag gtgtcatcgg
     5101 agaaattgct tcttgggtaa cctctccttc aaaagcactt cacgtagcag cagaagaagg
     5161 attacttcct gaatattttg caaaagaaaa cacacacggt gttcctgctc atttgatgat
     5221 cgcaaatggg atcgttgcaa caatctgggc agccgtcttt acattaagcg gcggggggaa
     5281 taatctttca tttcttgcag cgatgtcatt gactgttgta atctacctga tgttgtactt
     5341 tatgtttttc gcgggctatt tgaagctggt ttttaaagat gacagtcttg aaagagccta
     5401 tcaggttcca ggcggaaaag ttgtaaaagt aatcgttgct tgtttaggtc ttttgacttc
     5461 actttttgct ttttgtatct catttgttcc acctgctagt atcgcagctg gacagcatgg
     5521 gatttatgaa acgattctga ttgtttctta tcttatcgtc ttagttattc catttacgat
     5581 ctacgcatgc cgtcataaat tccagcatgc agaaaaataa aaaatgaaag ctggttattc
     5641 aaatggaagt aaaagaatta gaacaattga ttggaaaaaa tttccactat tatcaaacag
     5701 gacaagtggc ggattacatt ccagcattag ctgaagtgga tcccaaacaa ttaggtatgg
     5761 cgatttatga tttggataca aaacaactga ttgagggagg agacgccgat gtgcgctttg
     5821 ccatcgaaag catgtcaaaa gttcccgtat tgttattagc aatcaaagac aacggaatag
     5881 aaaaagtctt tcaaacaatc aacacggaac caaccggttt tgccttcaat tctatcatga
     5941 atatggaaat caatcatcgc aaacatccaa tgaatccttt tgttaatgca ggagcgattg
     6001 caacaacctc gttgattgcc ggtaaagatg cagatgaaaa atttgagcga atcttgtcat
     6061 ttatgaaaga gatctgtgat gatcctaata tcacattgaa tgaagaaatc tatcattctg
     6121 aatcaagaac aggtgatatc aatcgctctc ttgcttacta tatgaaaggt aatcaaatga
     6181 tcgaaggaga cgtaccggag atactcgatg cttatttcag acaatgctcc ctcaatgtca
     6241 ccgctacagg catagcaaaa ttagctgctg tgctggcaaa taaaggaatt gctccttgga
     6301 atggcaaaca aatcattact gaagaaagtg ctacaatcgt caaatcaatt atgacaactg
     6361 ctggtttgta tgatgaatca ggagagtttt ccgtccatgt tggtgtacct gcaaaaagcg
     6421 gtgtaggcgg cgggttgatg gcagctgtgc caaatcgtta cggcataggt gtcttcagcc
     6481 cggcactaga tccatttggt aacagcgcag ctggtatcca attgttgaaa gatgtagtca
     6541 aagaactaga cgctgacatc tttgaataac cattcttgaa agaggctggg gtaaaagtcc
     6601 aaacggctca aagctaccta caatgaggct gaaattgtga gcttgtttga cttttgtccc
     6661 agtttctttt tttattggtt tttgaaaggg cttttctaaa taaaaaggaa acaaaaatac
     6721 gatttacaga tatgattaaa taaaatcata agaaaaactg gaggcgtgta tcaggaatgc
     6781 tggaaaatta catcgagcga aatatattta gaaaagtgta tctgtgtgaa caattatttg
     6841 aattccaaga aatagacatc gaacaaacag ctatttctct aagggtgact acaccaacga
     6901 tccttcacga tctagaaagt ctagccgagt gtctagaata ttgtataaaa gaacaagtaa
     6961 gagagaagca taagtataaa ctcgtattca aacatggtat tgctctttct gagctcacac
     7021 agtttctgta cggtcaatct tattttttaa agtttttaag ttattatttt aatggtatat
     7081 tcacgagtac agaactggct gatcttgaat ttatttcttt gagtaaagta tatacaatca
     7141 aaaaaatcgt attggatttt tttaaagaaa atgattattt gaaaaataaa gaaatcatta
     7201 tcccagaatt tgactcgcgg aacatcttgc tagctttagt acgttatgtc aattgggaag
     7261 gctatgaaaa taaaaatccg tatgtagaag aagcttgtaa cgagttgatt gattatgtag
     7321 agactcattt ttttaaaaga agatattcgg aagatgaaaa gtcctttatc atacgagaaa
     7381 tagagatcgc aatcggaaga aaggcatttc cccttggact gaaagaagaa gataagaaaa
     7441 gcgcagaaac gaagccgtta tttcaattag tctgtcaagg attggaagag caaaaaggaa
     7501 ttctagactt acaagaagaa gatgtctatt atattttttc tttgttcaac agtagaaatt
     7561 ataccaatga gaacatggag ctgttaagaa aagatttcga agttgtttat aaaaattttg
     7621 ttctcaatga cctatcactg aatgaattga ttttacagat tattagtatc gctgaaatcg
     7681 acgatgcaga aaattttatt ttcaagcaat cctttttaca atttgtccgt acgttgtggg
     7741 cagatggaca agtattttta ccggaaagga tctatcttct atcagaaaaa gaacaacttc
     7801 tgtacggaaa aattgtagaa atattggaag agtggaaaaa gaaaaatcaa atccaactaa
     7861 gatggaatga aaatttcatt cgaaaattcg tcaaaagtct ttctttgatc aacagtgaac
     7921 aagcggggag acaggaaata gaaatttttg ttgtttcaga aagttcagtc aaacaatttt
     7981 tttatcgaac ccaatttcat ttgtatgcgg aagaaggcat cgctgaaata aatcctattt
     8041 tatttcgttc attagaagaa ttgcctgatg aatgtctatg tgcgaaaaaa agagtggttt
     8101 tgtgcgatgc tgctagctat caaaatggat tagaaactga aaagacaaag atctatccaa
     8161 tctcattaaa aggtgcaaac atccatttgt atcatattgc tcaggaactt cacctattaa
     8221 aaaaacatat ttcttgatga gaccttaatt aggagacctt ctacaaagct ttttaattgt
     8281 ctgtagaagg tttttatttg agtcctgatt cgtgcgaaga tctacttata taacaagctt
     8341 tctctgaaat ttttttaaaa tgctattgac ttagagtcaa ctctaagtag tagaatagat
     8401 aaagtgtaac aaatctattt tcattaaaag aagatagtac ggaggagaga atgtattatg
     8461 agtacagtat tacatgtatt ggatgcaatc aatagccccg cagatgtcaa aaaattatct
     8521 gttgaggaaa tgaaacagtt ggcagaagag atgcgccaaa aaatcttgca aaaggacagc
     8581 gatgtaggtg ggcacgttgg tccgaatcta ggtgtagtag aattaacgat tgcgtttcat
     8641 tatgtatttg attcaccaaa ggataaaatc gtttgggatg tttcccatca gtcttatggt
     8701 cataagcttt taactggtcg aaaaaatggt tttgaaaaag gacattaccg tgaagtgagc
     8761 ggatattcca accaacatga aagtaaacat gactatttta cagtggggca cacatctaca
     8821 tctatcagct tagcaacagg tttagctaaa gcgagagatc tgaagaaaga atcaggaaat
     8881 gtggtagcat ttatcggtga cggctcccta agtggtggct tagcttttga aggattgaac
     8941 aatggtggtg ttttacaatc caatctaatc gtattagtaa acgataatga gatgtctatt
     9001 gatgagaatc atggcagttt atataaaaac ttagctgagc ttcgtcaaac caacggtaaa
     9061 agcagcaata acctatttaa agcaatggga tttgattatc gttacgtgga taatggaaac
     9121 gatctggaaa cggtgatccg tttatttgaa gaagtaaaag atattgatca tccgattgta
     9181 cttcacgtcc atacggaaaa aggtcgcggt tatcagccgg caatcgataa taaaatgaaa
     9241 tatcattggc atgtaccatt tgatctgtcc accggggagg caaagcaagt aacttctgga
     9301 gaaacattta atatgatcat catggattat ttggataaga aaatgcaaac aagtacagct
     9361 cctattgccg cagtcaatgc agcgatacca ggcgcctttg gactgaaaac attccaagaa
     9421 aaacatccag atcattattt tgacgtgggg atcgctgaac agcactctat cactttcgct
     9481 tctgctttgg cttctcaggg agtacgtcca gtcgtcttcc aatcaagtac tttcttgcaa
     9541 cgggcatatg atcaattatc acatgattta gcgatcaacg agaatccagt gattatttta
     9601 gtcggtggcg gaacgatctc agctagtgac cgaacacacc aaggaacatt tgacattcct
     9661 tacttatcaa gtatcccgaa catcaaatat cttgctccag caacaaaaga ggatatgatc
     9721 tctatgctag attgggcgtt cgaacaaaca gcgcagcctg tagcaatccg aattcctaca
     9781 catggtgtag aacatggtac agctatcgca aatgattata gtattccagc ctatcaaaca
     9841 gtaaataatg gaggaaaagt agcaatttta gctttaggcg gttttcttga attaggaaaa
     9901 gaaacagtaa aagcttttag aaaaaaagga atcgaagcaa cgttgatcaa tccaggaacg
     9961 atcacagaaa atgacgtaga aacattagat aaattaaaag aaacacacga attagtcatc
    10021 acattagaag atggtagttt gtcaggagga tttggcgaaa atatcagtcg ttattatggt
    10081 gattcttcaa tgaacgtatt aaattttggt gcagagaaag aattcacgga tagcgttcct
    10141 gtagggcagc tttatgaacg ttatcattta acaccaaaac aaatcgttga agatagtctg
    10201 cgtgtcttga atattcaata attcattatg ctcaccatcc agataattgt ttatactagt
    10261 atctggatgg ttttttcatt ataaaaagca aaggggtgga tgttatgtat cagcaagtag
    10321 aagaatatgt caaaaagttc caagaaaaat atcctagtta tcaatcagaa aaattaggtt
    10381 tcttttcatt tggggataca gaaaaaatgg cagacgagtt gagcgaacta gttgtaaaaa
    10441 agataaagac tggtacatca agtggttacg atctgtataa acaagagaat gagcctttac
    10501 caaaagttgg tcaattagac gtggtcctga atggaaaaaa tgaaccagtt tgtatcatca
    10561 aaaatataaa tgtgaccatc ttgcctttta gtgaagtcac gaaagagcat gcttggaaag
    10621 aaggagaagg ggatcggaca ttagcttatt ggcggaaagt ccatgctgag ttttttaaac
    10681 atgcttataa agaagcgttg aacatagaat ttacagaaga gaagctggtc gtatacgaag
    10741 aatttgaagt aattttcttt tagtaagaaa ggtctgctga aagaaatagc tcattattgt
    10801 ctcctgaata gaaaagggtt acatttttat atgaagctgt atttttatat aacattactg
    10861 gaggagatat tgtggataca ctggaacaaa tcaaaggcgg agtcgtcgtt tcttgtcaag
    10921 ctttggaaaa cgagccgcta cacagttctt ttattatggg gagaatggct ttggctgcaa
    10981 aagaaggcgg agcagttggt attcgagcaa atacgacaga agatatcaat gaaatcaagc
    11041 gaatcgttga tttaccggta atagggatta tcaaaagaga ttacgatggg tcagaagtgt
    11101 ttattactgc gacagaaaaa gaagtggaag aacttttgga gacgcaagca gaaatcattg
    11161 cactggatgc aacttctcgt aaacgtccga cagaaaaaac aactgctgaa cttgtccaaa
    11221 tgattcacga acacggtcgg ttagctatgg cagatatttc gacttttgag gaagcgcttc
    11281 aagcgcaaga agatggtttt gatcttttgt ctacgacttt agcaggctat acagaatatt
    11341 cacgaaagac gcagacacct gattttgatt tattgaatca aatcatagaa caagtcgata
    11401 tcccagtaat tatggaagga catactgact ctcctgaaca agtagagaaa gcatatgaat
    11461 taggtgcttt ttcagtcgta gttggatcta ttatcacccg tccgcaattg atcactaaaa
    11521 gatatgtcga agccgcaagt aaagcaaata actaaaaaac acgcacggca cagattcttg
    11581 acaaaaaaga aaaattgata gatagtttaa agcagattct aagaagatga aaaaagcatt
    11641 acttttcatt ttgtcttcaa gatctgcttt ttaacgaatt ttatagaaag aaggcgaaac
    11701 catgtttgaa cggagtcctt cagaattgac agaacgtgaa aattacaagt tgctgattgg
    11761 ttcagtcatc cctagaccag tagcagtagt aacgactaaa tcagcaaaag atatagtgaa
    11821 tatcgcaccc ttcagttact tcaatatcgt cagttcgaat cgacccatct tatccttggc
    11881 gatccagaga aaacaagatg cagtaaaaga cacagcaaag aatattcttg agacaagaga
    11941 agctgttatc catatccttg atgaggacaa cgtagaagaa gtaaataaaa cagcagccaa
    12001 tctgcctaat gaggaaagcg aactgtacct gactgacttg actttaactg attcagtaat
    12061 catttcagta ccagcattga cagaagcaaa agttcgtttt gaaacatcct tatatgaaca
    12121 tgtcgagata cggaataaaa ataaagtaac agctgattta tttttgctag aagtacagaa
    12181 atatcattta aatgaaacag tctatgatga taagacagga aggatcgatc caagaaaatt
    12241 gaatgctgta agccgtttgg caggaaatga ttatgcagca ataggagaaa ttttcacgat
    12301 tgcacgtccc gaataaaaag gagcactagt tatgttaaaa ataagctata aaccatcgac
    12361 agattcaaaa gaaatgaaaa aagaatatga aacagtgaat gattttctgc aagggcagta
    12421 cttagaagtt ccgccattgc aagaccattt tgtcgttact actgttactt tagatggtaa
    12481 ggaaatagaa atgccagatc aaacgattag cggattgttt aattac
//
LOCUS       DACDIG010000075        12485 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.075, whole genome shotgun sequence.
ACCESSION   DACDIG010000075 DACDIG010000000
VERSION     DACDIG010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.075"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(60..1766)
                     /locus_tag="IV247_002291"
     CDS             complement(60..1766)
                     /locus_tag="IV247_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAP8778345.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            1956..2579
                     /locus_tag="IV247_002292"
     CDS             1956..2579
                     /locus_tag="IV247_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778346.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            3029..4078
                     /locus_tag="IV247_002293"
     CDS             3029..4078
                     /locus_tag="IV247_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8778347.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            4075..4956
                     /locus_tag="IV247_002294"
     CDS             4075..4956
                     /locus_tag="IV247_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8778348.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            4959..5729
                     /locus_tag="IV247_002295"
     CDS             4959..5729
                     /locus_tag="IV247_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8778349.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            6008..6862
                     /gene="aroE"
                     /locus_tag="IV247_002296"
     CDS             6008..6862
                     /gene="aroE"
                     /locus_tag="IV247_002296"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAP8778350.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            7040..8203
                     /locus_tag="IV247_002297"
     CDS             7040..8203
                     /locus_tag="IV247_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP8778351.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            8320..8730
                     /locus_tag="IV247_002298"
     CDS             8320..8730
                     /locus_tag="IV247_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP8778352.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            8904..11132
                     /gene="topB"
                     /locus_tag="IV247_002299"
     CDS             8904..11132
                     /gene="topB"
                     /locus_tag="IV247_002299"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP8778353.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            11270..11587
                     /locus_tag="IV247_002300"
     CDS             11270..11587
                     /locus_tag="IV247_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778354.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            11747..11983
                     /locus_tag="IV247_002301"
     CDS             11747..11983
                     /locus_tag="IV247_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP8778355.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            11980..12379
                     /locus_tag="IV247_002302"
                     /pseudo
     CDS             11980..12379
                     /locus_tag="IV247_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
BASE COUNT         4193 a         2115 c         2617 g         3560 t
ORIGIN      
        1 ttataactaa ttgctgttgt gggaaagtac gcaaatatga ttctagaaac actcattgat
       61 tacctgtttt gtttcatagc tgtaattttt tcttcttctg gtgtcacgat aatcgtcttt
      121 tggttttcat aaataacata accaggcttg gcgccatttg gttttcggat atgctttacc
      181 tgtacaagat caaccggcac ttgggcagag tatcggtatt ttgaaaaata ggcagctaac
      241 tcggcagctt ctgtaattgt ttcatctgag ggcttgtcac ttttaatgat gacgtgcgaa
      301 ccgggtatat tttttgcatg aagccaatag tcggtttttt tagctgtctt cattgtcagc
      361 tggtcatttt gaagattgtt tttaccaaca agaatgagcg ttccgtcaga agaaaagtat
      421 tgatcaggct gagatggttt cttacgtttt tgttttttta agttcttctt tttcaggtat
      481 ccttctgaag tcaattcttc tttaattgct tcgatatcca ttgggccggc aatttccagt
      541 tgagataaga cagattcaag atattggatt tcatctttgg cttcttggat ttgttcgcca
      601 atcagcttaa cagcgttctt cagcttttgg tatcgatgga aatatttctg tgcattttga
      661 tttggtgtca atgccggatc tagtgctatt ttgattggcc gatcttcttc ataataatta
      721 ggtaaaacaa cttcttttgc gccgcgtggt acttgtgtca gaaaagtagt aagtagttcg
      781 ccatttctac ggtaattctc agcgttttcg gattctttga gtgtttgttc acgttttttc
      841 aatttatttt tatttcgttt tagttcattt tcgatttttc gaatcagttc tccgccttgt
      901 tgttttgccc gatctttctc tgctttttcg tgataatagc tatctaataa ttgactgagt
      961 gttggataag ctgttacagc agatgcctgt tcggacaata cctgataagc aatcggtgtg
     1021 aaaaaatctt tattttccgt ttcgtaaaaa gtaggaatag gctgatggct gatggctgag
     1081 aaaaattcgt cccatacaac catcttttca ttaggacgtt ccagtaatct tttggtcagc
     1141 tcttgagcga tatcgaaacc gattccctga aactgacgtt ggattgcttt tggatctagt
     1201 tcagtttggg aaagccgctg aaagattttt tctttctcgg agctaaatgg gttcaagacc
     1261 tcttgttttg gcggagcaac atattctaca ccaggaagca aagaacgata agtgttttga
     1321 gagcttccaa tgtgtttgat cgcatccagt atttttccag tttctcgatt aactaagacg
     1381 atcgtactat gacgtcccat taattcaacg atcaatatga tattttgtag atcacccaac
     1441 tcgtttcgtt tagcaaagtg gaaatggatc actcgatcat tttcgatttg ttcgatgtct
     1501 tctaaaatag cgccatccaa atattttcga agcatcatca cgaagttagg cggattatca
     1561 ggattctgat aatcgatttg ggtaatctgt acacgagcat aacttgggtg cgcagataat
     1621 aataatcgat ggtttttgcc gcgtgaacga atcaccagca cgatttcatt ttcataaggc
     1681 tgatggattt tcgagatccg gccagatagt aacgtttcac gcaattcgtt gaccatcgcg
     1741 tgggtaaata caccatcaaa ggacatggtt cattcctcat ttcttttcat aagtatattc
     1801 attataacga aaaaaagaag aaaaaccaat ttggccgaat agtaaagcaa aagtctagaa
     1861 aaatcttgga ctaattacat aaaaaacagt gtttctatcg ttgaaatcca aatttttagg
     1921 tataataaaa aataggagaa aaagtagaaa agaaggtgtt tgttatttgg gaagtagtta
     1981 aatcgagagt acaagaaatg aatgaacatt tctcagtgtc attcgcagaa aatccaagta
     2041 agtattttcg acgtcttgtt tttctgctca tcttatcatt gctgttaaca gttgcaatgt
     2101 ttttattgcc ggaaaaaatc cgagaagagc aagtaaatag tctcgtttat tcgtccatac
     2161 caaccagcaa attgaaacct tttgattaca gcaaagctga tcagaaaatc aaacaaagcc
     2221 gggcgatatc ggtgatgttc agcaaaccga acgggaaaac ctatcaggac atgcttgatg
     2281 tttttaatga tcctgagctt atggaagaat tgaatcgacc gatcttttat tatccgatcg
     2341 tttatgatgt acatgaactg gaacaaaaat ataacatacg aacggatgaa gtgacattta
     2401 ttttctttga tggaggaaaa gaagcaaatc ggataactgc gggtaaggga ggaataactg
     2461 attttgataa ggagttgatc cctgaactga atcgacttcc gttagccaat atcaaacaat
     2521 tagaagaaga attggataga acgggagtaa cttcccaaaa ttccgagatt gctaattgag
     2581 acattggcgc gatgaaacgg tgggtctata tagcgctatt tttaattatc agaacaagtg
     2641 atgtttgatc ttcaaaataa caaataaaaa attaaaaaat atagttgacg aaattcaaaa
     2701 attcgattag acttaaatca attcttaagg aaagggtgac ctgtaatgaa cttatcaaaa
     2761 cgtatttcag cagaaaacaa aaaaaattgg cgcagctggc gtagataagt tgtattaatg
     2821 gtgagacttt cccatagata caacttttta gagtgcattt ctaaagcttg tatctatgct
     2881 agacatgaac attgactgcc gcatagatta gaatctatgc ggttttttgt gtaaagacaa
     2941 tgaccgcttt ggccattgtc tttttttgta gactggatac ttgcctgatt ataataaata
     3001 aaaagattga ctttgcagga ggaaaaaaat gaaaaacaaa cgattaataa cagttgtagt
     3061 aattatttta gtttatttag caggtgcatt tatttatgga aaagcctcag gcgaaaaaga
     3121 aagtacttcg acgaaccaga caaaaaaaac aacagttgtt ggggtattgc aatacgtgag
     3181 ccatccggca ttagatgaga tttatcgagg aatcaaagat ggtctgaaag aagaaggatt
     3241 aaaagaagga gaaaatcttg agatcaaatt tcaaaatgga caagccgatc agagcaagct
     3301 agccacaatg agtcaacagc tagtacaatc tgatccagat gccttgattg gaatcgcaac
     3361 acctgccgct caatcattag caaacacgac ttcatcgatc ccagttgtgt taggtgctgt
     3421 aacggatcct gtgggggctg gattagtcgc ttctttagag aaacctggtg gtaatgtaac
     3481 aggtgtttct gatcagccgc ctgttacttc acaaatcaag cttgcttctg atttgttgcc
     3541 aaatgcaaaa aaagtcggaa tcttgtatgc ttcttcagag gataattcaa aataccaagt
     3601 agcacaagca gaagaagctg caaaaaaaga agggttagaa tcagtaaaat atgcggtgcc
     3661 ttccactaat gagattgcac aaacggttca agtgatgagc cgacaagtcg attttatcta
     3721 tgttccatta gataatacca ttgccaatgc gatgcaaaca gtggtaaaag aagcaaacaa
     3781 agcaaatatc cccgtcattc catctgtgga tacaatggtg gaacaaggtg gactagcaac
     3841 aatcgggatc aatcaatatc aattagggct tcaaagcggt aagatggcgg cagaacttgc
     3901 gagtggaaaa gaaaaaccgg aaacgacgcc tgtttataca tttgatcaag gggatacagt
     3961 gatcaatcaa tctcaagctg atcgcttagg aatcattgtt ccccaatcga tgaaggaaaa
     4021 agccaaaatc attacagatg actcgcagca agaaacatct aaggaggatg acaaatgatc
     4081 gtatcagcta tttcacaagg catgctttgg gcaatattag gattagggat tttcatgact
     4141 tatcggatct tagactttcc tgatatgacg acagaagggt ccttcccatt aggtgctgct
     4201 gtctgtgtga cagctattac tcatggggta gcgccgattg tagctacatt attaggcgta
     4261 ggtgctggca tgatagcagg actcgttacg gggttacttt atacaaaagg aaagataccg
     4321 gttattttag cagggatttt agtgatgtca ggattgaact cagttatttt gtttgtgatg
     4381 cgaacaccaa atctttcttt attgaatcat ccgattctac aagacagctt ccatcgcttt
     4441 gggttacctg attactttga cacagtcctt cttggcttca tctctttggg catcttgatt
     4501 gctgtactgc tattcttctt caatacagat ttgggtcaag gctatattgc gacaggagac
     4561 aacgaaacga tggctcggtc gttagggata aaaaccgatc gtatgaaaat tttaggttta
     4621 actttgtcga acggtgtcat tgcattatcc ggtgcgctga ttgcccagaa cgacggatat
     4681 gcagatgtaa ataaagggat tggcgtaatc gtcatcggtc ttgcttccat catcatcggt
     4741 gaagtgatct tccaagaatt gacgctagca gaaagattga ttgctattgt tgtcggcagt
     4801 atcatttatc agctattgat tttagtagta atcaaactag ggtttgacac gacttatctg
     4861 aagttgttct ccgctatcat cttagctatt tgtctgatga tcccgcaatt gaagaaagca
     4921 ctgaacttaa aaacgggttt tgagaaggag gcatagtaat gactgatata ttgacaatca
     4981 aaaatggcag aaaagtcgta aacaatggaa tgaatgaaga gaaagtacta ttggatgaaa
     5041 ttaaccttac aatcagagaa ggggacttca tcactgtcct tggcgggaat ggtgctggaa
     5101 aaagcacact gttcaatacg atcgctggta cgcttcaact cagtcaagga tcgatctctt
     5161 ttaaagatca gctgatcaca aaagattcag aagaaaaaag agcacgattc ctttctcgag
     5221 ttttccaaga ccccaagatg ggaacggctc ctcggatgac agtagcagaa aatttgctac
     5281 ttgcacaaaa aagaggtcaa aaacggaaat tccgtttacg taacttgaaa aatcaaaaag
     5341 aagatttttt cagactatgt caagaagtcg gtaatggcct agagcaacac ttggatactc
     5401 cagcgggtga attatctggt ggtcaacgcc aagcattaag cttgctgatg gcgacgatca
     5461 cagctcctga gttactatta ttagatgaac atactgcagc tttagatcct aaaacagcta
     5521 aagcattaat gcgattaaca aatcagcgga tcaatgaaca aaaattgact tgtctcatga
     5581 tcacgcatcg aatggaagac gccttgaaat acggaaatcg tctgatcgta ctccaaaaag
     5641 ggaaaatcgt tcaagatatt tctgaaaaag aaaagcaaca tttgacaatg ggcgaccttt
     5701 tgcagttctt tgaagaagta gtggattgat ttcgacagaa aaattccttt cagtagaagg
     5761 acttgaaaac tcattgacat cttcctttga ttcatcctat acttaaaaca ataaacaatt
     5821 aaacatattt gggtgttttg taggatcacg tagtcaatga ttttaacaat taaatagaat
     5881 attctttgct ctgacaagta aacgatgaga aaagaatttg ctactttgcc acctagacgt
     5941 ttcgtacttt ttcgtatcaa acgctaagtg gcttttttta ttgaagtaag aacaaaggag
     6001 gaataagatg atttctggac aaaccaaatt agctgggctc tttgctgatc cggcttctca
     6061 cagtttatcc ccattgatgc acaatactgc ttttgaagca aacaacatcg atgcggtgta
     6121 tttagctttc caagtaaatc caatgaattt gaagcaagca gtcgaaagta ttcgaacttt
     6181 tgatatgtta ggtgtaaatc tttcgatgcc gaataaaaca gcggttattc cttatctgga
     6241 tgaggtaagc caggaagcgc aattgatcgg agcagtgaat acgatcgtgc acgaaaaggg
     6301 tcgtttgatt ggctataata ctgatggtgc gggcttcatg caatccgtgc aagaagctgg
     6361 gatttcgatc aaaaagaaaa aaatgactct actgggagct ggaggtgcag caaaggcagt
     6421 cactgttcaa gcagcgttag acggcgctga ggaaatcgta gtttataaac gaaacaacca
     6481 aacattcgct caagtaaaag aaacattcga acaactcagc tatcgaactt cttgtaagat
     6541 aaaagttcgt gactttgccg atacgcatca attaagaaaa gacttgaaag aaagctgtct
     6601 tctagtcaat gcaacagata tcgggatggg aaagaaaatc aatcagtcac cgctcttaga
     6661 tataagcatg atgcatgaga atctagcggt ttttgatttg atttatgcac ctagagaaac
     6721 acgtttatta aaagaagcaa aaaaagtagg agcaaaagga tacaatggac ttggcatgtt
     6781 gatctaccaa ggagcaatcg cgtttgaatt atggacaaag gaaaagatgc cgatccatgt
     6841 gattgaaaag ctgtttgatt aaaaaaggat aatagataaa gatcttttat tctcgcataa
     6901 acggtctcaa gaaaagtaat cctttatgta tcgaagcatt tatttcgtta ttttttatta
     6961 aattatcaaa aaatgatagg cttttgtcga ttggttatcg tatcctttat ttatcagtaa
     7021 gaaacgagga ggaaccacaa tgagtcggat ggatcgctat aaggacatcc ataagaaggc
     7081 gaaacctctg aagcgggata cggggagtat ttttgctaga agagaacgaa aacgaacgcg
     7141 tatggaaaat gaggaagatg ctacccgtat ttatgaaaga gagcagcatt catttgaaca
     7201 tttgaaccaa tacgagcaac agatatacag agatgcacca agagcagaag aacaacaaaa
     7261 aaataaaaaa tttttcaatc ggcaaaagaa gaacaaaccg ttaaaacctc gaagaaagcg
     7321 atcgtggaaa aaaatcattc tatcgatttt gcttgttttg atcctttatt caggtatctc
     7381 attttttatt ggtaaatatg tagctgaaca tgacacaagc ttagaggcag cgacttcaga
     7441 aacctttcat gggcaaaaag cgacgaatgg tgcgcataat attttgatcc ttggaagtga
     7501 tacacgcgga gaagatgccg ggcgagccga tacgatcatg gtcttacagt tggatggtcc
     7561 ttcccataag ccgaaactga tttcttttat gcgtgacagc ttcgttacga tcccaggggt
     7621 aggacaaaat aagatcaatt cagcttatgc atacggagga gccgatctag tgcgtcaaac
     7681 tttagtagaa aattttggaa tcgattgtca atactatgcg aaagtcgatt tcaaatcgtt
     7741 tgaaaaagtg atcgatgcct tattcatgaa cggagtgaag atcgatgcag aaaaagatct
     7801 gaatctggat ggtgtagata ttaaaaaagg gggccagaag atggatggac atgttctttt
     7861 gcagtacgca cgtttccgaa tggatgaaca aggagacttt ggccgtgtca gacgccaaca
     7921 gcaagtaatg aacgctattt tcagccaatt gaagaatcca ttgaatctga tccgtgcacc
     7981 ttatgcagct ggtaaagctt taggatatac atccacagat gtcactgctt ggttcttgac
     8041 gaaaaacctc ttttcgattg cacgaggagc tggtggcatt gatcgaataa gtgtgccagt
     8101 agacggttcg tggaattatg ggcaaagtga ttatgctggc agtattcttg aaattgataa
     8161 tcaaatgaat caatctgcta ttacagattt tttgaataaa tgatcataaa aaagatcaag
     8221 aagaagctgc gcaaggcttt tgtcatgaat caatcaaacg attcactcgt aaaaaacgct
     8281 ttcttatggt agatttaacc aagagaggag ctgtttacat tggatatcta tctgaacttc
     8341 aaaaatgagt cgcgtgaagc catcgctttt tatgaaaaag tttttcagac tgaagcatca
     8401 ggtatcacca catacggtga aatgcctgga atagaacaaa tgaacttgcc agaagaagca
     8461 aaaaatctaa tcacgaatgc acggctgaac atttatgata cattagttat gtttgcagat
     8521 acgccagaag gaattggtcc tgaattagtt gtgggaaata atgtttcttt agtcatccag
     8581 acaagaaatg aagaagaaac gaagcgccta tttgaaagct tagctgaggg tggtaagatt
     8641 gagatgccac tggatgctgt tccgtgggct cagttgtatg ggatggtaac tgatcgattt
     8701 ggcatcaact ggcagattaa ccaagagtga cagctaaaga tgaatatttt ctggaaacat
     8761 caagcagcaa acggatcatc gtttgtttgc ttgatgtttt tgtgtctatt tcgactatac
     8821 ggtatctttt ttttattgcc agatgcgaaa atatgttcca taatagagaa gtccgcaaaa
     8881 aattaagtaa cggaggtcag aagatgaaga cggtgatact agctgaaaaa ccttcacaag
     8941 caaaagccta tgccgaaacc ttccaacagc aaaagcggca tgaagggtat tatgaaatca
     9001 gcgatccatt gtttgctggt gaagtaacca tcacttatgg ttttggacat ttggtagata
     9061 tggttgctcc aggagcttat gaagagcgtt gggcaaaatg gtctttagaa aatttgccta
     9121 ttttcccaga aaccttccgt tacgaagttc caatggacaa acgtgcacaa ttttcgatcg
     9181 taaaacgaga attgcagtca gccgatacca tcattattgc aacagatggt gatcgagaag
     9241 gtgaagccat cgcgtggtcg attattcgac aagcaaacgc attttctaag gaaaagaact
     9301 atttacgttt atggatcaat tctcttgaaa aagaagcaat ctatgaaggt tttaagaatc
     9361 tccaacctgg aaagaactat tttccaaaat ataaagaagc gcaagcaagg cagaacgctg
     9421 attggctgat tggaatgaat ggaagtccat tatatagctt gctacttcaa cgaaaaggga
     9481 ttgatggcag tttttctctt ggtcgtgtac aaacaccgac actctatatg atttatcagc
     9541 tgcaagaaga aatcaaacac ttcaaaaaag aaacgtactt tgaaggagaa ggtttaatta
     9601 cgacaaacaa agggcagttt tctggaaaaa tcgtaccaaa aaaagcattt aaaacccaag
     9661 aagagttaac gaaggaaata gaaaaacttg gagcgcattt aggtaagcaa aacggacgga
     9721 ttcttgaagt tacgaaaaag gaaaagaggt tgcatagtcc acgtttattt tcgctatcta
     9781 gtttgcagac aaagatgaat cagctaatga aagcaagtgc caaagctaca cttgatgctg
     9841 ctcaaggatt gtacgaaagc aagtttctaa gctatccaag aacggattcc ttttacatta
     9901 cagaaaatga gcatcagtat ctggttaaac atctgcaaaa ttataaaaaa tttttaggaa
     9961 tagaagaagt acagacaact gagatcaaag caaagaaacg atatgtcgat gcgaaaaaag
    10021 ttcaagaaca tcatgcaatt attccgacaa agacgattcc gactcctgca cgctttgcga
    10081 agctttccaa attacagcaa gcaatctatt tgcaagtttt gcgcactact gtggctatgt
    10141 ttgctgcaga ttatatctac gaagagacga ccgtaatgac gggcgtacag caattaaagc
    10201 taaaaagcat cggaaagatc ccattacagc aaggttggca ggaaatctta cgcatcaagt
    10261 caaaaaagaa agaagctcag actcttcctc ctatcgtaga aaatgaagaa gcagaagtag
    10321 aactgaaaag tatggagaaa gaaacacaac ctccaaaacc ctataccgaa ggaacgttga
    10381 tcacagcaat gaaaactgcg ggaaaaacgc tggataatga tgaagcgcaa gaaattctca
    10441 aagaagtaga aggaataggg acagaagcta cacgagccaa tatcattgaa aatcttaagc
    10501 agaagcatta catcgaagtc aataaaaacg aaataactgt tacgccaaaa ggaatcactc
    10561 tttgtaaggc agtagccaat gaaccattac tgacgagcgc agaaatgact gcgcgctggg
    10621 aagggtactt gagaaaaatt ggtaaacaag aggggacacc atctgttttt ttagagaata
    10681 tcaaaaaatt catcctacat ttattggcag aagttcctaa tcaaatcgac aaggtagact
    10741 tttcagatga gattagcggg acacgtcaga taaaagcgat ggagaagaaa aacgatcaat
    10801 taggaagatg tccaaaatgt aaaaagggaa tagtcatgct ttatcctaaa atcgctacat
    10861 gcttgaatcc ggaatgcgac ttcaaattgt ggccaacagt agctaaaaag aagctgacta
    10921 aaacgaatct cagagatttg ctttcaaaag gaaagacaag taaagttgtc aaaggtttta
    10981 ctggtaaaaa agggaaattc gatgcagttt tagtattaaa agaagatatg acaatcggtt
    11041 tttcttttcc atggatcgaa aacagtggaa caaaagcagt tgatggaaac aaagaaacat
    11101 caagaaaaaa taatgaaaac gaagtagagt aaatgaaaac aatgaaatag cggaatattt
    11161 attccgaaaa gtgagaatat aaataccaat aaaactaaaa aatatcagtg aatcaagcaa
    11221 aaacgttggg gtaggctgat tttatataga tagcaaggag tttttttatt tgaaaaaagt
    11281 gcattcattt ttaaattatc ttatgggagc aatcacgatt gtggctgtgt accaagcatt
    11341 tctaaagtat cctgattacg ggacgaagtt gttgtcattg acgcacaatc aaacagcata
    11401 ttcaccaacg ttgattttat cgactttgac tgcaggagct aaaagcgcta cttatttttt
    11461 tcataaaacg acaagcatgc taaagcgaaa gaaacagcga aaacaattgt tggtggtggt
    11521 agcggctatg atcattattt attttctacc agttattaca caagtatttc atcaatattt
    11581 tggttaagaa tacgagagtt tgtgacaaaa gtcatgtcac aaactttttt ctttgtcatt
    11641 ttttcgtaag aaaaagcaaa ataaaagtat atacattgat taaaagtata tacttggata
    11701 taatgtactt actatagaat gagaatggta tagagggtgg cgaaatatga aaaaaatgaa
    11761 aaagtataaa attagaaaat ctggcaatag cgatgtaaca actatcccac cagaagtaaa
    11821 agaattcatg ggtgtgcaga caggtgacgc aatcagttat gtttttcaat ctgatggttc
    11881 tgtacgaatg atcaaagcac aagaagaacc tgatattgat tcattagttg attcgatcat
    11941 gaatcaatat gaagacgcgt taaaagattt ggttgattta tgaggtatct ttcgactaaa
    12001 gaaattatca aaatcaacgc gaaagttatt ttaccgtcac tcgccaggcg aaatgatcgg
    12061 tgtaaaagat gcaaatgcat tagatatggc cgtcaaacag ccttcacaag cagtattcaa
    12121 tcaagaactc tatcctgaaa tctatgaaaa agcggcgatt ttagcaatta atttagctaa
    12181 aaaacatcct tttcataatg gcaataaacg aactgctctt gttgccatgt tgttgtttct
    12241 tcaattgaat aattgtccag ttgctttttc tcgtcaagaa gctgttgatt ttatcataat
    12301 gattaccact agttcattgg atttcgattc actaaagtcc aagataacca gttatttacg
    12361 acaaaccgca ataaaatgaa cgaattaaaa actttatttc tcaattagat cagcatgtca
    12421 ttaaggaata tgctgatttt tttatgttta atcatacaaa atagtttttt cataaaaata
    12481 tggaa
//
LOCUS       DACDIG010000076        12225 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.076, whole genome shotgun sequence.
ACCESSION   DACDIG010000076 DACDIG010000000
VERSION     DACDIG010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.076"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(178..735)
                     /gene="frr"
                     /locus_tag="IV247_002303"
     CDS             complement(178..735)
                     /gene="frr"
                     /locus_tag="IV247_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAP8778356.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(738..1460)
                     /locus_tag="IV247_002304"
     CDS             complement(738..1460)
                     /locus_tag="IV247_002304"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAP8778357.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1596..2477)
                     /locus_tag="IV247_002305"
     CDS             complement(1596..2477)
                     /locus_tag="IV247_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAP8778358.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2576..3358)
                     /gene="rpsB"
                     /locus_tag="IV247_002306"
     CDS             complement(2576..3358)
                     /gene="rpsB"
                     /locus_tag="IV247_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAP8778359.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTVTSIEEIVDVVEGDNSSAE"
     gene            3717..4196
                     /locus_tag="IV247_002307"
     CDS             3717..4196
                     /locus_tag="IV247_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAP8778360.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4413..5504
                     /gene="phnW"
                     /locus_tag="IV247_002308"
     CDS             4413..5504
                     /gene="phnW"
                     /locus_tag="IV247_002308"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAP8778361.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5497..5625
                     /locus_tag="IV247_002309"
     CDS             5497..5625
                     /locus_tag="IV247_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP8778362.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5629..6174
                     /locus_tag="IV247_002310"
     CDS             5629..6174
                     /locus_tag="IV247_002310"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP8778363.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6628..8319)
                     /locus_tag="IV247_002311"
     CDS             complement(6628..8319)
                     /locus_tag="IV247_002311"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAP8778364.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8739..9686)
                     /gene="arcC"
                     /locus_tag="IV247_002312"
     CDS             complement(8739..9686)
                     /gene="arcC"
                     /locus_tag="IV247_002312"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAP8778365.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9801..10820)
                     /gene="argF"
                     /locus_tag="IV247_002313"
     CDS             complement(9801..10820)
                     /gene="argF"
                     /locus_tag="IV247_002313"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAP8778366.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10911..12140)
                     /gene="arcA"
                     /locus_tag="IV247_002314"
     CDS             complement(10911..12140)
                     /gene="arcA"
                     /locus_tag="IV247_002314"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAP8778367.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3350 a         2531 c         2205 g         4139 t
ORIGIN      
        1 ttaaaagatt cccactatct taagtattat agcaatatct aactgttttt cctatgaaat
       61 cattgttttt ttatttaaaa actgaataat tttttctttt tttaattttt aaataaaaga
      121 aatataaata aaaacagagg aagcggaatg atccgcccct ctgtttttat gattttatta
      181 aacttccaat aattcttttt ctttatcagc agcgatctta tctacttctt tgatgctgtt
      241 atctgtcaat gtttgtacgt ctttttctaa tccgcgcaaa tcatcttcgg taatatcacc
      301 atttttttgt tgttttttat attcatcgat cgcatcacga cggatgttac gaatcgcgat
      361 ttttgcattt tctgcttctt ttttcacatc tttagctaat tcttttctgc gttcttctgt
      421 cagttgcggg ataactaagc gaatgacatt tccatcattg gttggactga ttccgatatc
      481 acttgccata atagcttttt caacatcttg aagaatgctc ttgtcaaaag gtgtgatcat
      541 taaaacacgt gcttctggaa tttggattga tgccatttgg ttcaatggtg ttggcgcacc
      601 gtagtagttc acagtaatac ggtccaaaag gctagcgttc gcacggcctg cgcggatttg
      661 cccaagttca cgttgaagat tttgtgctgc ttttttcatt ttttcttttg cttctgtcat
      721 aattgcatct gccattttta tttccccctt acagttgttc cgatattttc gcctaagatt
      781 gcacggcgaa tatttccgtg ttcatttaaa ttgaagacaa gcagtggaat atcattatcc
      841 atgcttagtg aacttgcagt tgaatccatg acttgtaatc ctttggcaat aacttccaga
      901 tgagtcagtt cttcaaattt tactgcattt gcatctactt tcggatcagc agaataaaca
      961 ccgtctacat tgtttttagc catcaggata acatctgcgc cgatttctgc cgcgcgtaat
     1021 gctgctgttg tgtctgtcga gaagtacggg ttgccggtac cgcctgcaaa gataacgatc
     1081 cgtccttttt ctaaatgacg ttctgcttta cggcgaatat atggctctgc gatttgacgc
     1141 atttcaatcg aagtttgcac gcgtgtcggt acacccacgt tttccaacgt atcctgtaaa
     1201 gctaaggcat tcataactgt agctaacata cccatgtaat cagcttgagc acgttccatg
     1261 cccatttgtg ctccgatttg tccgcgccag atatttccgc cgcccacaac gattgccatt
     1321 tcaacgccca gctcgtgaac ttctttgatt tcttggatga tttctttgat tactggtggt
     1381 ttgattccaa atcctgcgtc cccagctaaa gcttctccac tcaattttaa gactacacgc
     1441 tgatatttcg gtgtgaccat gttcattcct ccaatatttt ctattgccat tttaccatac
     1501 ttagatgatt tcgacactct aagactggtt tttcttgttt tgcttaaaaa aagggagcgc
     1561 acaaaccgtg cgttcccgtc tgttattcaa cagaattatt ttttcacttg gctcataact
     1621 tcttctacaa agttatcttc gcgtttttcg ataccttcgc caacttcgaa acgtacgaat
     1681 gttttaacag ttgcgccttt tgatgcaacg tatttttcaa cagtcatatc aggatcttta
     1741 acgaatggtt ggtccaccaa agcgatttct gctttgaatt tcttcaagcg tccttctacc
     1801 attttttcaa cgatattcgc tggtttgcct tcgttcaatg cttgttctgt aagaacagtt
     1861 ttttcatgtt ctaattcagc ttctggaatt tgtgattcgt ttacataacg aggattgata
     1921 gctgctacgt gcatagctac gtctcttgca actgtttcgt cagttgttcc atctagaaca
     1981 gtcaatacgg caatacgtcc gcccatatgc aagtatccac caaatgcagc attgtcgtct
     2041 ttttctacaa cttcaaagcg acggaagctg attttttctc cgataacttg agttgcttcg
     2101 atcaagtcag attcaattgt acctttatct gttttgattt tcattgcagc ttccatgtct
     2161 gctggtttgt tttcagcaac aagttctgca atttctttta ctaaatcttg gaacatttcg
     2221 tttttagaaa caaagtctgt ttctgagttt acttcaacga ttgctgcagt gttacctttc
     2281 acagcaactg aagccaatcc ttcagctgca atgcggtcat ttttcttcgc agcctttgcc
     2341 attccttttt cacgtaaaag atcaactgct ttttccatgt cgccttctac ttcaactaat
     2401 gcttttttcg catccatcat tcctacgcca gtcatttcgc gtagttcttt taccatttta
     2461 gctgtaacgt ctgccatgtt taactcctcc tgtgtgtttt tctttttaaa aaaagctgct
     2521 tcaaaggaga gtgaggcttg tcgcccagcc tttgaaacag ctccatcatt caaaattatt
     2581 ctgctgaaga attgtcgcct tcaacaacat ctacgatttc ttcgattgat gttactgtgt
     2641 tttcttctgt gaaatcttct tcaactactt gatcttcacc ttggtttcct tcgatgaaag
     2701 catcagccat ttttgaagta atcaatttaa cggcacgaat tgcatcgtcg tttgaaggga
     2761 ttactacgtc gatttcatct ggatcgcagt ttgtatcaac catagctacg attgggatat
     2821 ttaatttgtg tgcttcttga acagcaatac gttctttacg tgggtcaacg atgtacatta
     2881 catctgggat tcttggcata tcagcgatac cgcctaagaa tttttcaaga cgttcacgtt
     2941 ctttgttcaa accagcaact tcttttttag gtagaacttc aaaagttcca tcttcttcca
     3001 ttgcattgat ttgtttcaaa cggctgatac gtttttggat agtatcccag tttgtcaatg
     3061 ttccgcctaa ccaacgatgg ttaacgaagt attgtcctgc acgagttgct tcttctttga
     3121 ttgcttcttg tgcttgtttt tttgtaccta cgaataatgc tacgccgcct tcttcagcaa
     3181 cgctcttcat gtaatcataa gctgcatcta ctaatttaac tgttttttgc aagtcaatga
     3241 tgtagattcc gtttctttct gtgaagatat atttcttcat ttttggattc cagcgacgag
     3301 tttggtgacc aaagtgtacg ccggcttcta gtaattgttt catagaaatt actgccatgt
     3361 ttgtttcctc cgatttggtt ttatttttcc tcttcttatc ttctaccttg ccggcaaact
     3421 ttttcaagca cctaccgaca atccaataag aatgtggatt tgctgatgca taaagcaccc
     3481 tcagctatta tacagacaaa agatccgtat cgcaagagca aatatacttt tctgctcaac
     3541 attcaatagt ttacctgaaa gataaaaaga attcaagaaa aacagtagat ttctctttgt
     3601 ttctttatta tcctttttcc ataaaataat tttaggtata aaagacgaat attattgggc
     3661 aatccttctt ttttttgtta tgatacatgt gaaatctttc gtaaaggaga aagaaaatgc
     3721 gcaaagctca aagacatttg ctgattaaac aagtcattga agaattcccg atccgtacgc
     3781 aagaagaact attaaacaaa cttgggcatt atggcgttac cgccacgcag gcaacgattt
     3841 ctcgagatat ccgtgatcta aaaatcgtga aggcacctga tgaaaatggc gtttcccgct
     3901 tcgtattgtt tcgcgaaaaa ggaactcctg agactaggac tgaggaagag gatcggctgg
     3961 ttcaaatgat tgaagacatt gtattgaaag tcgaacgagt ccactttttg acgatcgtca
     4021 ataccttgcc agacaatgct catttatttg catctgtttt agatgagatc aaacctccct
     4081 ctattgtcag tacaattgct ggattcgata cgatcatggt catctcggaa aatgaggagg
     4141 acgcaaaacg agtagaagaa tttcttcacc atccaactga agtatcactt ttttaatcag
     4201 agcacgtatt cctaatatgg aatgcgtgtt tttttatttt caatattttc cttattgaat
     4261 aaacctttac actctttaca aaaatacatc attttttatt caaatttttc aacaccttga
     4321 ttgaatcaca ataatcatca tttatattgg atgttaacaa gagcagcttc ttgctctttg
     4381 tgtatttcaa aaaagaaatg aaaggaaatc atatgtataa attattaact tctggaccat
     4441 tgacgacttc tgaaacagtg aaaaatgcta tgctgatcga tcattgtact tgggatcaag
     4501 aatacaaaga aattacacaa tggataagaa aaaaattatt ggatcttgct caagtatcgg
     4561 aaggaatata cacaacggtc ttaatgcaag gtagcagctc cttcggggta gaatcggtat
     4621 taagttctgt gatcaggcca gaagatactg tactgatttg ttcaaacggc gcatatagac
     4681 aacgaatgat caacatgtgc gaatgtctga aactgaacta taccgtttat gaggtatcgg
     4741 aacatcaaat cctccaagct catgtgatta cacagctgat tgaagagaat ccgtctatct
     4801 ccgcactagc aatggtccat agcgagacta cttccggtat tttgaataat ttggaagaga
     4861 ttgctgctgt cacacaagaa agagatcttt tgtttatagt ggacgcgatg tcaagctttg
     4921 gtgggatacc gatttgtgta gccgaactag ggattgattt tttgatttcc agctcaaaca
     4981 aatgtattca aggtgttcca ggcttttctt ttgtacttgc taataaagcg gaactggaaa
     5041 aacgaaaaaa tgttagccga agtttgtcac ttgatctata tgaccagtac cgcgtgatgg
     5101 aaaacgagaa tggcaaatgg cgtttcactt cccctaccca tacggttttg gcttttcaac
     5161 aagcgctgaa ggaactagag gcagaaggag gaatcgaaac tcgatattca cgttatctcg
     5221 ccaataatca tgtgctacga caaaaaatga cagagatggg gttccagcct ttcattcaag
     5281 aatatcaggg gccattcatc acctcttttc tttaccctaa ggactggcga tttgatttta
     5341 atcattttta tcagtattta aagcaaaatg gatttgtgat ctatcccggg aaaatcagcg
     5401 atcatgaatg ttttcgtatc ggaaatattg gcgagatcta tccggaagat attttggaac
     5461 tgacagcaat tatcaaagac tataaggaga acgttaatga ttaaaactgt aatttttgat
     5521 tgggcaggaa caactgtgga ctttggctgt atggctcctg tccacgcttt tagaaatgct
     5581 tttctagaaa aaggaatcca actgactgac aaagaaatcc gctaaccgat gggaaaatta
     5641 aaatgggatc atatccaaca gcttctttcc ctcccttctg tcaaaaaaca atggatagct
     5701 atttatggac aactacctca ggaaaaagag gtggaggaat tgtatcaaca atttgaacat
     5761 tatctgttta aagatttggt caagcattct accttgaaac ccgacacatt ggaaacgatc
     5821 gaacggttga gaaacgctgg gatcaatatt ggttccacga atggatttaa tgctcctatg
     5881 atgacaatcg tagcagaaac cgcaaaaaaa gaaaggtatt caccagactt catggcaact
     5941 tttgaagacg tcgaagggta tggtcgaccg tttccttata tgatccataa aaacatgcag
     6001 tacttcaaaa acaagtcggt tgatgaagta atcaaagtcg gagatacggt ctcagatatc
     6061 caggaaggga aaaatgccgg agtgttgaca gtaggtgtaa ttgaaggtag ttcgctaatg
     6121 gggatttctt atgatgagta ccaaaaatta gagatgggga aaaagatcgt ttaaaacagt
     6181 ctgttgctct tagattccaa caagccggcg ctgatatttg tgtaaacaat ttaaaagagt
     6241 taacagaata cattttgtac gctgacaaga atccagaatg acttttatac aaaataagag
     6301 gttgtgaaaa agctgtcctg tatcaagtaa taaaaacagc caagcaaaaa tagctcctca
     6361 tatttttcgt ttggttaggc ttattaccgc agatgcaagc ttttgaacac cgtttattcg
     6421 aaaaaaaaaa gggagctgtg acacgacttt tgtcacagtc cccttatttg caaacaaaca
     6481 gtgtttaaga gacaacctct ccactaaacg gtgttcattc agctgactct cggtatttgc
     6541 tcaaaaaacg ataaaatttg aaaagctatt ttctcttttg tttctctaat actcggatca
     6601 gaacgctgat ttcacattct ctttttctta catcttatct ggtgcatgaa gtcctagcaa
     6661 acgaaggtct tctttcaata atacagtcac tgcataaact agagctaagc gtgcttcttt
     6721 ttgttcatca tcagctaaaa tcttcgtatg agcatagtat ttgttgaatg cttgtgctaa
     6781 tttgattgca tgtttcgcaa tcactgacgg ttcgtatttt tccccagcag acaagaccgt
     6841 ttcagggtat ttttgaacta atttaaccac ttcccagctg gtatcgtcat tcaatgcata
     6901 gtctgctgtt tctgaaggga caaatcctgc tttttccaac aggctgactg cacgagcatg
     6961 tgtatattgc acataaggtc cagtttctcc ttcaaatcga acaacttctt ccaggttaaa
     7021 gtcaaatgta ttcagtcgat cgtttttcaa atcgtggaaa atgactgccc caaccccaac
     7081 ttgtttagct acagcatctt tgttttccaa atcagggttt ttctcgctga tttgttcttt
     7141 agcagattgg attgcttcat ttaatacttc ttctaataag acgatctttc ctttacgagt
     7201 tgataatttt ttaccacctt gcgtgatcaa accaaatgga atatggtgca tgtcgtcaga
     7261 ccagtcaaag cccatttcct tcaagacagc tttcaactgt ttaaaatgat agctttgttc
     7321 attccctact acatatagag attgtttgaa gtcgtaagta cgtttacggt acaatgcagc
     7381 agctaagtca cgtgtaatgt atagtgtcgc accgtctgac ttcttaatca acgctggatt
     7441 caggtcataa gcagataggt caacgatctc tgcaccttta tcctcgttca gcaaatgttt
     7501 ttcttctaaa agtttgacga tttcatccat cttgtcattg tagaaagctt caccgttcaa
     7561 cgaatcgaaa cgtacttcca gcagatcata gattttattg aattctttca tcgattcatc
     7621 acggaaccat tgccataatt gaatagcctc tttatcgcct tcttccagac gtttgaacca
     7681 agcacgagct tcttcattta attctggttc tgtttcagct acttcatgga attggacgta
     7741 taaacgaagc aattcattga ttggttctgc tttaacagct tcttctgtac cccatttttt
     7801 gtaagcgacg atcagttttc caaactgtgt tccccagtca cctaaatggt tgatgcggat
     7861 tggctgataa ccgatttttt ccataatgaa accgatcgaa ttaccgataa ctgtcgaacg
     7921 caaatgtccc attgagattg gtttggcaat attaggagaa gacatgtcga ttggtactgt
     7981 tccttgattc ccgatgttgc tatccccata atgttctttt tctttcacta cggtttgcaa
     8041 aacttttttg ctgataagtt ctttattcat gaaaaagttc aagtaaggtc cgacaacttc
     8101 gattttttca aagttggcag aatcgatttt ttctgctaaa tctgcagcga tttgctgtgg
     8161 cgcttttcgg taaacttttg ctaatgaaaa ggctggaaat gcaacatctc cgtgttcagc
     8221 tgattttgga ttttctagta attgctcgac ttgttccagt gttaaatcat ctttgacagc
     8281 gtcatagaca gcttttgcga caagatcttt attattcatc tttattcctc ctaaagtgtt
     8341 cccttgattg ttaaacaaaa aaaatctcta gcgacggcat acttagtatg ccatcactag
     8401 agacgagatt tcccgcggta ccactccaat tgtcctaaaa aaggaccagc tccatctgat
     8461 atcggtagac gaccgtctgt ttaaaacaga attctccaag tgcgcttcgt tgtatcttct
     8521 tcattcggct tccaccactt ccgaattcgc tagccaagaa tgatacaagt actctcttgt
     8581 tcaacaaatc ttagtcttat taaactttct tgaattatat cagaaaaaaa aacgaaaggc
     8641 tagtcgtttc ataaaaaaaa aatccagatg gcctaaagga gaagaaatac cacctggata
     8701 gctataaatc atttcgtaaa tacgaaagac ttaacaaatt aatcagctac gataatcgtt
     8761 ccttcttcag aagctaaaag attctcgata ttttctaaag aagtgatgat tgccttagcg
     8821 tttggttttg cttcaacaaa tgcaatagct gcttctactt tcggaagcat gcttcctgga
     8881 gcgaattgct tttcatcaat gtattgtttc atttctgaaa cagtaacagt ttctaatttc
     8941 ttttgatctg gtttttggta attgacatac acattgtcta ctcctgtcaa tacgatcagc
     9001 aaatccgcat cgatgatttc ggctaatttt tctgaagcaa aatctttatc gattacggct
     9061 tctcgtcctt ctaatccagt agctgtttct acaacaggaa tcccaccgcc accaacagag
     9121 actgtgataa ctccttgatc gaccagcgtt tcgatgacac gtgcttcttt aatcgagatg
     9181 ggtttagggg aagcgactac ttttctccat ccacgtccag catcttcttt gaatgtcaca
     9241 gtggagtcag cattcatttg ttctttggct tcttcttccg tgtagaatgg accgactggt
     9301 ttacttggat ttttgaatga aggatcattt tcatccacaa taacttgagt gactaaagaa
     9361 accacatctt tatcgatccc tttttccttc agtacttcgc ccatggcgtt ttgtaaccag
     9421 taaccgatag atccttctgt cattgcaaca catgtatcta aaggcattgc cggtgttttc
     9481 tcagaatcag cagcttgctg ctgaatcaat aggttaccga cttgcggacc atttccgtgg
     9541 gagatgatca attcatctcc ttgttcgata aatttcacta aatactttgc tgtttccatt
     9601 aacgcttctt gttgggcttt tgcacttgca tccgtagaca agatggcatt gccccctaaa
     9661 gcgacaacta cttttcgttt ggccatttca ttcgtccttt cttcagttct accgaatttt
     9721 ctagataaca aaacgagcca gcgttttgtg attttccctc aatgctgact cgttttttcg
     9781 tgagatgaat gtggttatgc ttaaacgcga gggataaaca agttacctaa tgttgctgcc
     9841 atgatcgctt tgattgagtg catacgattt tctgcttggt caaattgacg agcatatttg
     9901 ctgcggaaga cttcatctgt tacttccatt tcagtgatgc caaagcgttc tttcatttgt
     9961 tcaccgtaaa cagtgtttgt atcatggaaa gctggcaagc agtgcaagaa gatcaaacga
    10021 tctgtgttgt gtgttttttc taccatttcc atattgattt ggtaaggttt caacaatttg
    10081 atacgttctt cgaatttatc ttcttcaccc attgataccc atacatcaga ataaaggacg
    10141 tctgctccgt ctacaccttt atcaacatcg tcggtaatca tcaattgtgc accggatttt
    10201 tcagcaaagc cttctgccat tttgacgatt tcttcttcag gttgtaattc ttttggtgca
    10261 acgatacgca tattcgctcc taaaattgca cctgtgacta ataaagagtt agccatattg
    10321 tttcgaccat ctccgcagta agcaacagtg ataccttcta ctgttccaaa gttttcttgg
    10381 atagttaaaa agtcagcgat catttgtgtt ggatgccatt catcagtcaa gccattccaa
    10441 actggaacac cagagaattc tgctagttcc tctaccattt tttgactgaa tccacggaat
    10501 tcgattccgt caaacatacg tcctagtact ttagctgtat cttctgtgga ttctttttta
    10561 cctaattgga tatcatttgc acctagatat tcaggatgtg cacctagatc gatcgctgca
    10621 gtagtaaatg cagaacgagt acgtgtagat gttttttcaa ataaaagcgc aatatttttt
    10681 ccttctagat agtgatgtgg gataccacgt tttttcaagt ctttcaagtg ttctgaaaaa
    10741 tcgatcaaat attgtaattc ttcttttgta aagtcttttt ctgctaataa gcttcttcct
    10801 tggaatactg attctttcat attaaacatt ctcctttaga ttgtttttat ttaagattaa
    10861 agaggaaatc ccgacggcag ctgacagtaa gactgccgtc aggaaacaaa ttacttcaag
    10921 tcttcacgga caagtggttg gctcatgcag cgtggaccac cacgacctcg tgacaattca
    10981 cttgaattga tttctaacac tttgatgcca tagctacgaa gcaattcgtt cgatacataa
    11041 tttcggttat aagtgactac aacacctgga gcgattgcca atgtgtttga tccatcattc
    11101 cattgttctc gaggtgcaac gatctcatct ccattaccag ttgggatcag tacaagatca
    11161 tctaatccaa gagcagcttt catcgtttcg tgtaagttat ctgaatgagt aatcttgata
    11221 tcatcaccat ctggttcgat tgtaaagaca tccatcttac cgtctttact ttgaattgct
    11281 ggatggatag tgaatttatc gtgatctacc attgtaaata cggtatccaa atgcatcatt
    11341 gcacggttgt tcggaatttt gattgccaac actttttcaa atcctgaatt ttttgcgaat
    11401 aggtggcgtg ctaatttttc taaagctttc gcattagttc gttgagaaat cccaacagct
    11461 actactttat cgcttaagat caattcgtct ccgccttcga tatgatctgg attttcacga
    11521 tccaaccata cttcgattcc tttatttgca aatcttggat gatgtttcaa aatatattct
    11581 gtaaacatcg attcacgacg acgtgcttca aaagtcatct tattgactgt catcccattt
    11641 cccattgatg cggaagggtc gcgtgtaaag tacaagtttg gcattgggtc catgtaaaat
    11701 ggatagtcat cgtctgctga cagatcatat agactgctag aacgtacttc gatatctttt
    11761 gtacgaaccc cagccatgat tttatctacc atttcttgcg tttccattga tagaaggaac
    11821 tcatgaagac cctctcttac agcattcgat gcaatatgag attcatccaa cattttattc
    11881 aagaattgtt ctttgacatt gcctgcgtca atcgcttctg ccgctaattt ttccaagtaa
    11941 agagtctcga cgccttcatt ttgcaatgtc ttcgcaaagt tgtcatgttc ttcttgagca
    12001 atcggaagat atggaatatc atcgaacaac agacgatcca taatatctgg tgtcaaattt
    12061 tccacttctt gtccaggccg tttcagtaaa actgtcttta atttccctat ttcagagaaa
    12121 acgtgaatag gtttatccat gttgtgttgc ctccttcttt ttgattacat gtataggata
    12181 tcctcctttt gtaatcactt tcataatttt gtctcattca aaatg
//
LOCUS       DACDIG010000077        11732 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.077, whole genome shotgun sequence.
ACCESSION   DACDIG010000077 DACDIG010000000
VERSION     DACDIG010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.077"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(284..2068)
                     /locus_tag="IV247_002315"
     CDS             complement(284..2068)
                     /locus_tag="IV247_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8778368.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQEFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            complement(2068..3819)
                     /locus_tag="IV247_002316"
     CDS             complement(2068..3819)
                     /locus_tag="IV247_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8778369.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLERELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            complement(3961..4185)
                     /locus_tag="IV247_002317"
     CDS             complement(3961..4185)
                     /locus_tag="IV247_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAP8778370.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            4436..6022
                     /locus_tag="IV247_002318"
     CDS             4436..6022
                     /locus_tag="IV247_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAP8778371.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            complement(6088..7863)
                     /locus_tag="IV247_002319"
     CDS             complement(6088..7863)
                     /locus_tag="IV247_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAP8778372.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(8074..8976)
                     /locus_tag="IV247_002320"
     CDS             complement(8074..8976)
                     /locus_tag="IV247_002320"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAP8778373.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            9255..11600
                     /locus_tag="IV247_002321"
     CDS             9255..11600
                     /locus_tag="IV247_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP8778374.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKNEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
BASE COUNT         3557 a         2404 c         2186 g         3585 t
ORIGIN      
        1 acccaaaaag ccgtccatgg aaccatggga gactcatcag gtttagcttg catcaggcaa
       61 tcactatcca agttgtatcg ttttctttat attatcgaaa gcaaacgctt ttgtcaacgc
      121 tttcttagtg gttattagcc gcttttgact agctgtcgtc gtctttttta aatcgggtga
      181 ctacgccact aacgctcaca gatgaagaat ctgcgaagct ctgttttatt ttcactctag
      241 cgcttagggc aaaatgctac tatgctactg tcacaacctc tttttacaca ccactgcttt
      301 gcagtttgta catatctgca tacaaaccat tttgcgctag aagtgtttca tgattcccac
      361 gctctactat atgacctttg tctaaaacta aaatcaattc tgcatcccga atcgtggaca
      421 gccgatgggc aatcgctatc gttgtccgtc cttgtcgcat ttttgccaat ccttcttgga
      481 tcaatccttc ggtttcagta tcgatatttg ctgttgcttc atccaatact agtattttag
      541 gatctgtcac gattgttcgc gcaaaagtga tcagctgtcg ttgtccacta gaatagctgg
      601 caccgtgttc gatcactttt gagtgatatc cttttggtaa ttcatcgatg aacttgtttg
      661 cttgaacaaa ctcagctgca tggcgaatct gttcgtctgt gatagctgga ttcatcatcc
      721 ggatatttcc tgcaatatct ccataaaaca agaaggcatc ttgcaagacc aatcccattt
      781 tcttacgtaa ctcttccatt ggatattctc gaatgtcatg atcatcaatc aagatttggc
      841 cttcataaaa ttcgtaaaac cgcatcaata cgttgatgat cgagcttttt ccgcttccgg
      901 tgtgccccac taatgcaacg gtctcacccg gattagcgac aaaagagata ttgttcaata
      961 cattgtgctc tccatcataa gaaaagctta cattgcgaaa ctcgatctta cctgaagtaa
     1021 tcgcttgatc tgctccagga ttttgttgcg gtgcatattc ttctgtatcc atgattttca
     1081 aaatacgact accggcaacc atcccatctg taaaggtgct caaaaagtcc atcatttgag
     1141 acattggatt aaaaaattcc tggacatagg tcgtaaatgc atagatcagt ccaacttcta
     1201 cgggagaatg caacgcatcg atcccaaaca aagtcaacgc caacgcaatc gccaatgcat
     1261 agagaaaatt gatgattgga cttaacagaa tcgaattcgt tttgatcatc gcatagcgcg
     1321 tgtctaaata ctcgttgttc acttgttcaa attctttagc taaacgagct tcttggcgaa
     1381 actgctggat aatacgcatg ccggaaatcg attcattcaa tttcgtgttc agctgactta
     1441 atttttcccg catatttcgg taaatacgag aactgaattt ctggtaatac cagatgacga
     1501 ttcctaaaat cggcaagaac accaaagtcc acaaggcaat ttctccattg atttgaaaca
     1561 tggcaacgaa agaagaaacg actgcaaaga tcccagtcaa taccatcaaa aagacatacc
     1621 aaaattcaaa tagcgtttct gtgtcattcg tcactcgaga aacggtcgat cctgccggtg
     1681 tctggtcaaa ataacgcata cctagtgtat gcagcttttc aaataatctt acccgaacat
     1741 actgataggt cttcaatgaa gccatggaat acaaatacca ttggaaaaac caaatgatac
     1801 ttttgactat gactccaaac aagtacacac cagcaaaaaa caaaataact tgtgtcgtag
     1861 ctgactgttt agccagatga tcatccataa aagtttggat gatccttggc aataggacat
     1921 tgatcacaga cagggcaaac gcaaataaga tagcaacgat gaacgttctt ctaaaaggtt
     1981 tggcgaactt catcagtcgt ttgatgatct tcttctgttc tttaagtgga atcgattttg
     2041 tccattccga ttgataattt tcatccatca ggcctcgcct ccttctatct tcgcttctaa
     2101 ttgctgtttt tcccacattc gttcatacca gccacctaat gcaagcagct cgtggtgggt
     2161 tcctcgttca atgattttcc cttgatcgac aacaatgatt tcttttgcat gcatcacgct
     2221 gcttaaacgg tgagctgcaa tgatggttgt tttctctttt cttatttctt ttaaattatg
     2281 caagatagct tcttctgttt tggcatctac tgcagaaagt gcatcatcta agatcagcaa
     2341 ttcaggattt ttgatcaatg ctcgagcaat cgaaatcctc tgtttctgtc cgccagataa
     2401 agagactcca cgttctccaa ctaaggtatc ataacctttt ggcaattcct tgatttcatt
     2461 gttgataaat gctaaagaag ccgcatgctc tacttcttct tctgaaagtt ttggattaga
     2521 aaaacggata ttatctcgaa tggtcatcga aaacaggaag tgatcctgag gcacataacc
     2581 gattgatcct aataaagcat tcaatgaata ttctttgatc gaacgtccgc caaattggat
     2641 tttcccttga taatcatcgt attcacgcat cagtaatttg atgattgtcg tttttcccgc
     2701 accagttttc ccaacaatac ctaatgtttc tccttcttgg atcgcaaatt tgatttgttc
     2761 taatgctggt gattcttctt tcggatagtt aaaagaggaa atagctacat tcagctcgcc
     2821 ttttgccggg gtctgcactg cacccagttg ttccacgata tgcgtctttt catgcaaaag
     2881 ttcattgacg cgatcataag aagcatttcc tctttccaat acgttgaaca atcgaccaat
     2941 cgcaaacatc ggccaaacaa gcataccgat atagctaata aatgagacca tctgcccgat
     3001 tgagatcgtg ccttccaaga catactttcc acctaaaata attgttgcaa cataagacag
     3061 accgataatc aaggtaatga atggatcaaa taaggagtct aaaaaattga ctcgtttgtt
     3121 tttaataatt gcatcttcaa tcttttcttc aaaatccgcg agatcttctt tttcttgtcc
     3181 aaatgtttta atgactttga tcccagtgat actttcttgt gtcttatcat tgatactaga
     3241 aaaagcttct tgcgaatcac gaaaagcatc gtggagtttt gaccccagca ctcgtgaagt
     3301 aacggctagc aaaggcagag gaatcagcgc aatcagtgtt aagcgccaat caataaacaa
     3361 aatcatcgca atgatcgttg ttccgcccgt aatcactgaa tctgcaaagg tcaagattcc
     3421 agctcctgct acattctgga ttgcattcaa atcattcgtt gcatgagcca tcaaatctcc
     3481 agtccgatat ttttggtaaa agagattgtc catttctgta aaatgtttga atagctgctg
     3541 ccgaagttct ctctccaacc gagctgcact tccccaaata ttcgtccgcc agatataacg
     3601 aaaaagatat tgtaagaaag ctgcaaggat caataataag atccaccagg aaatgatctt
     3661 tattgataaa ttcttatcgg cgatctcatc aatgatgatc ccaatcactt ttggaggaac
     3721 taactgtacc aaggccactg cgaataggga gaaaatccca attaaataat gtttcttttc
     3781 ctgacgaaaa aaccaaccta attttctaaa aatcgacatt ttactcctcc tttgtcttta
     3841 tcaagagcat aggattgtct gtgcttgtct tttttgctga atagtctgaa tagtctaaca
     3901 aattacgaaa taaaaaacgg actgaaaaaa tcagcccgca aaaagtcttt gggtcagaac
     3961 ctattttttc ccttggttct tcatttgctg catcatctgg cgaattttct tctcagatgg
     4021 tttttgcccc attgacatca tcattgatcg caacatgtcc tcattaacgg gaggattttt
     4081 ttctaaatag tctttcatat atttacgcgc taggaaaaag ccacctactg cgccgattag
     4141 caaagcaata atagcaataa gtacgacaat acctgttgat accatataaa tctctccttt
     4201 ctatttccga attgctctca atgatttatt ttactaaagt ttattgaaaa aagaaaggcg
     4261 tattctttag tttcatgcaa aaaatagtgg tttttttaga gaagaacaaa atttctttca
     4321 ttaaccagca agacacacca cgaaaaaaaa ttaggcatac cgaatctttt tctttaattt
     4381 tttctttctg aggtgtagaa tgtaatcgta gataaatagg aaaggtggtt ttttcttgaa
     4441 taatcaatcc aacaaacaat ttcataagca tcgacttatt gaacatacaa atggtttatc
     4501 tttagctgaa gtaaatggaa cagtggatgt accaaaggga aaaagttttt ttaaaacact
     4561 tcttgcttac tctggaccag gtgcattagt agcagttggt tatatggatc caggtaactg
     4621 gtccacttcg atcactggtg gacaaaattt ccaatattta ttgatgtcag ttattttgat
     4681 gtctagtcta attgctatgc ttttacagta tatggcagca aaattaggaa tcgtgaccca
     4741 aatggatttg gcccaagcaa tccgagcacg gacaagtaag acattgggtg tcattttatg
     4801 gatcttgact gaattagcca ttatggcaac agacatcgca gaagtcattg gtgcagcaat
     4861 cgccctctac ttactttttg atattccatt ggtgattgcc gtctttatta ctgtattaga
     4921 tgttttcgtt ctcttactac tgacaaaagt cgggtttcgt aagatcgaag cactggttgt
     4981 ctgcttaatt ttcgtcattt tattcgtttt tgtctatcaa gttggcttgt ctaacccaga
     5041 ttggcacagt ttatttgctg gctttatccc taacagtcat acattctcag actcaccggt
     5101 catcggcggc gaaactccgc tgactggagc tttagggatt attggtgcaa cagtcatgcc
     5161 gcataactta tatcttcatt cagcagtttc acaaactcgg aagatcgatc accaaaacga
     5221 agaagatgta gcacaagctg tacgtttttc tacttgggat tctaatatcc agttgtcaat
     5281 ggcttttcta gtcaatgcac tgctattgat catgggtgtc gctgtcttca aaaccggttc
     5341 tgtccaagat ccttcctttt ttggtctgta tgaagcttta tctgatccat cagctatgag
     5401 caatggtatt ttgattgctg tagcaaaatc tggtatcttg tccactttat ttgctgtagc
     5461 acttcttgct tctggccaga actctactat tactggtacg ttgaccggtc aagtcatcat
     5521 ggaaggattc atccatatgc gtatcccgat ttggctaaga agactggtca cacgattgct
     5581 ttctgtgatt cctgtattga tttgtgtttt gtttacaagt acaaaaggaa cgatcgaaga
     5641 acatgccgct ttgaataact taatgaatga atcccaggtg ttcttggctt ttgcgttgcc
     5701 attttcgatg attcctttac tgatgatgac caacagccaa gcggagatgg gtagccgatt
     5761 taaaaattca ttcatcgtaa aacttcttgg ctggttctct gtgattagtc taacttactt
     5821 gaacttacga ggattaccag gacagatcga aagtttcttc ggcgagcaag ccacttccgc
     5881 tcaactagca ttggctgatc acatcgctta tgtaatcatt gttcttgtcc ttctcctgtt
     5941 ggcatggacg attggtgatt tatataaagg aaacaaaaaa tatgaacagc ttttgcagga
     6001 aacggtagaa gaaatcgaat aaaaactgat ttttccacac aaagaccctt gatagttagg
     6061 ttaaaaatct agctaccaag ggtcttttta tttagaagat aaagcttttt tcagatttaa
     6121 tttactgaaa actagcatgc ttgttactac gatgaattgc gttaatggca cgcctgcacc
     6181 agtgtagaca cttaaaaaat agtccttttt ttctggcgca taagaacaaa accatgagac
     6241 accgaaaaga aaaaccaatt gtgtcagtat taatccggtg accacaatca aagaggaact
     6301 ataataggca cgatcagctt ttgcaaatac ggttcctagt atcataagta atatccctgc
     6361 tattgaagca atcagcacgg gaatagccag aacttggtaa atttttatga tgacattcgc
     6421 gacttttgct gattttgtta attggaaatt aggtgttctc ggttcattac tatcgatcca
     6481 attctccatt agcggaaggt ttaataattc ttgagcctgt ttgacttgtt cttgcgttcc
     6541 gaaactataa tttcctcctt gatggaaatc tttatatcgc catgcataag cgtatcctga
     6601 ttgaaggaaa tcgaatacga ttggcagatc actcatcgtc tttccatctc ctgatcccat
     6661 cagatagaac tcttttttct ttttgatggt tcccttgtga taggcttctt ctaattcttg
     6721 gttgactttt ttccaaaaat cattggtttc ttttgcattg ttttggtaat aaccagattc
     6781 gcttaccaca tctcgcaatg cccaaaagat gatatctccg ggtaattctt ttacttttcc
     6841 accttgagtc cacgcgtgag tcgtatataa ttcgtcaata cgggtagaaa cagtatggaa
     6901 tgtaggagat gcggctaatg ctttatttag tgcagcatgt gagacccata tctcactgtt
     6961 ttctttattc aagttcgttt catcatctat tcggatcagt tgtttcatca aacgtccaaa
     7021 ttctccactg gaacggtcat tgatggttgc aataccgtat gttgtctcat ttttatattc
     7081 gatccactga tagacgatgg aaaagccaac tataggaagt aaaaacaatg aaagtttcag
     7141 ccattcccgt ttcgagagaa gaaagagtcg tttttttaac ttctttactt ctttgaaaga
     7201 aaagtcttgc cctagcaaga tctggacgaa actgataccc aaagctacta cagtaaaagg
     7261 aagaagccag atagaatctt cgcgaatgaa ccaaaagaaa ggaaaagcga cagaaagccc
     7321 taaaaaccat ggcagcaatt ttctgctaga accgtatttg cggtaataaa tagctaataa
     7381 acaagcgata atgatctcaa cagccggaat cacgagggca ttacgatatg ttcgagtgga
     7441 atattcatgt gtaaaactaa tcggagaaaa caaaaaataa aaatagatac ttaaaagaag
     7501 atacttgttc ttcacaatcg gtttaaacgc gagtgcaacc aatatactgg caaaaatact
     7561 gaacatgccg aacaaaactg cataagaaag atgcagttta tgtgcaagta ctaaaaataa
     7621 gctgtaggag attcctttgg aaagtgttgt agtcgtatac tcccctagcc atttaccatc
     7681 caaaatattt tgagcatagt gaatcagcag ctgatcatca taggtcgccg acatatctgt
     7741 tccatagatt gctttactat ataaatacaa ccgaataatt gtaaataata cggctatgcc
     7801 aagaataaag tatgtatgtt tcttttttag ttggtcgatc aaggggttta aaatgttcag
     7861 catcttttcg tcctttcaat cacgttttgc tacttgtttg tgaaactttt cgttttttac
     7921 ttttctatca gtataccctg tttgtctgaa tttaaaaaga gggggcaaaa ggagcatggc
     7981 gaaaacgaat gcaaaaagca ctcctttgtt tcttaaacaa aaaaggacaa ggcagactgc
     8041 ccaatccttt tttgttgctg tcaggtagaa aacttaaagt gtgacttctt ctgctaataa
     8101 ctcagggaac acttcgggat cgaatgcttg cgctttgaac atggaaattt cttttccata
     8161 tggtgcgtaa gctttctttt tatctttacc gacatatggg gtttctaata ttttcgaaat
     8221 gttgtcaaac attggatcat ggacgatttt gttcaatgtg tcaaatccaa ttgttccaaa
     8281 accgatattg gcatgccgat ctttatgaga accttgtgga tttttagaat cattgatatg
     8341 cagtacttta agccgatcta atccgatgac cttatcaaac tcttccatta cacctgaaaa
     8401 atcttctttt atattatatc cggcatcatt cgtatgacat gtatcaaaag taacggataa
     8461 tttttcattc aattttactc catcgatgat ttgcgctaat tcctcaaatg ttcgaccgat
     8521 ttccgtacct ttccctgcca tcgtctccaa agcgatctgt gccgtctgtt cttttgtcaa
     8581 aacttcattc aacccctcaa taattctagc aatcccagct tctgcccctg ctcctacatg
     8641 agcgcctggg tgaagggtga tttgagtagc acctaaagct tccgcacgga caatctcttc
     8701 tctcagaaat tgtacagcaa atggaaacat ttctggtttg atcgtatttc ccaaattaat
     8761 gatataaggc gcatgaacga cgatatttgt tagtccgttt tctgccataa atgcttttcc
     8821 ggcatcaata ttcatctctt cgatagcttt tcttctagta ttttgaggag cgcctgtata
     8881 gatcataaag gtcgaagatc cgtaactggc tgcttcttct gccgcgccta acagcatttt
     8941 ctttccgctc atgctgacat gtgagcctaa taacatggat ggattcctcc ttgaaaggca
     9001 attgtttcga acaattcctg tatattgata agatgatgta caaagaaaat ggtttgtttt
     9061 tctttgccct gttctccata gtaactgaaa ctttccctaa agaaaagctt aagaactctt
     9121 tcataaaaaa gatcatttcc tcacgtttta tctcattcga aaaagaaatt cctgtcaaaa
     9181 acagccttgt ctgttttttt gtcctattat ttgctataat tagcgaaata aaaccattag
     9241 ttgaggtgac ccttttgcca cgcaaaaata ctcgaaaaaa acagaataga agaaaaaaag
     9301 aaaaatggtt tgttcctaag attattttta gagtgtttca atcattgact gtttttatta
     9361 ctgttcttct tttgttgttc gcagctctag gtatcggaat aggtgctggc tactttgcct
     9421 atcttgtcga agacacacaa ttgccgacta aaaaagaact gcagacagaa ctaggaaaca
     9481 ttacggaaac gtcaaagatt gtttatgcag ataatacaga gatatcaaaa atccagacag
     9541 atctgatgcg gacgactatt tcatctgata agatttcgcc tttattgaaa accgcgatta
     9601 tcagtacaga agatgaatac ttcgataaac atcaaggtta tgtccctaaa gctgttttac
     9661 gagcactagt ttcagaagcg actggcatcg gctcgtctgg aggatccact ttgacacagc
     9721 aattagtcaa acaacagatc ctaaccgatg aaacaacatt caaacgtaaa gcaaacgaga
     9781 tcgttctttc tgcacaagta gaaaaacact tttcaaaaaa tgagatcatt actacgtatt
     9841 tgaatgtctc accatttggt cggaataata aggggcaaaa tattgctgga gtccaggaag
     9901 ctgctagagg aatctttggt gtagatgcaa aggatgtgac tctgccacaa gctgcttata
     9961 tcgctggttt gcctcaaagc cctatcacgt atagtccata cacgaataca ggcgcgctaa
    10021 aagaagatct ttctgccggt cttgccagaa aagattttgt tttattcagt atgtaccgtg
    10081 aaggacaaat tacgaaagaa caatacgaag aagcgaaagc ctatgatctt acaaaagact
    10141 ttctcccaca acaaatcgcg gaacagactg acagagaatt cttgtattat accgtgatga
    10201 acgaagcaac aaggattatt gcacaacaat tggcagaaaa agataatgct gatatgtcag
    10261 attcatcggt cagtgaagct tattatcaaa aagcacagca aacgatccaa aacaaaggat
    10321 atacgatcca ttcgacgatc gataaagata tctatgctgc attacaaacc ggcgtagaaa
    10381 attatggcta catgttagat gatggtgctg gtagccaagt ggagactgga aatgtattga
    10441 tggataacag aaccggacgt atctatggct tcgtgggtgg tcggaattac agccagaatc
    10501 aaaataacca cgcctttgat acagaacgtc aagctggttc atccatcaaa ccggttcttg
    10561 tttacggtcc tgcaatcgat atgggacttg tcggatcaga atcccgtgta tcagattatg
    10621 caacgacttg gcaagaagga aaaaatgctg gtgagaaaat cgtcaatgcg accaatgaag
    10681 ggtcgaacac cttccaaacc gtccgtgagt cactggaatg gtccaacaat atcccagctt
    10741 atcacttata tcaagatgtg ttgaattccg gtggatcgaa acaatacgct tacgagcact
    10801 acttggcgaa gatgaactac ccagcaaatg ataattgggg cgttgaatcg gctcctttag
    10861 ggacagtaga tgtaacgacg cttcaacaga caaatggatt ccaggcctta gcaaatggtg
    10921 gagtctacga agaaggctat atcattgatt ccatcacaga taatgcaggt aatgtgattt
    10981 ataaacatga aagtaattct gtccgtattt attccgaagc aacggcatcg atcatgaatg
    11041 acatgatgcg tagtgtgatc aatgaaaaaa tcactacacc attcaaagat gtcatctcaa
    11101 gcttaaacgg aaatctagga aatgcagatt gggtcggaaa aactggttct accaatgaat
    11161 accgagattc atggctgatc gtctcaactc catctatcac tatcagtagt tgggcaggac
    11221 acgatgacaa tactggaatg gacagccaag caagaatacg ttcggctaat tatcttgcca
    11281 atctaatcaa tcaagcttac caagcaaacc ctgagatttt cggcactgat gagaaatttg
    11341 acttatcatc cgatgtgatg aagaaaaaag tatctgggtt cacaggacaa acagctggaa
    11401 aagttactgt caataaaaaa tccatccaaa cgccaggaaa aacagtggag tcactttggg
    11461 caaaaaatgg tccgaagaaa agcgccttta aattcggcgt aggcggtact gatgaaaact
    11521 atacagatta ctggaacacc gcttcagctt atgctagagc gaatccagag accaaagaat
    11581 cagacgataa agatgattga tctaatagat taatcagaat taaagggagc tgcgacaaaa
    11641 atcttgtcac agctcccttt ttccgaataa atggtgttca aaagcttaca tctacgatat
    11701 taagacaaat ttatgtaaaa atgtgaaaaa cc
//
LOCUS       DACDIG010000078        11492 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.078, whole genome shotgun sequence.
ACCESSION   DACDIG010000078 DACDIG010000000
VERSION     DACDIG010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.078"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..2061)
                     /locus_tag="IV247_002322"
     CDS             complement(<1..2061)
                     /locus_tag="IV247_002322"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAP8778375.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQ"
     gene            complement(2063..2980)
                     /gene="glyQ"
                     /locus_tag="IV247_002323"
     CDS             complement(2063..2980)
                     /gene="glyQ"
                     /locus_tag="IV247_002323"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAP8778376.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            complement(3364..4176)
                     /gene="recO"
                     /locus_tag="IV247_002324"
     CDS             complement(3364..4176)
                     /gene="recO"
                     /locus_tag="IV247_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAP8778377.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4319..5218)
                     /locus_tag="IV247_002325"
     CDS             complement(4319..5218)
                     /locus_tag="IV247_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAP8778378.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5233..5634)
                     /locus_tag="IV247_002326"
     CDS             complement(5233..5634)
                     /locus_tag="IV247_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAP8778379.1"
                     /translation="MPMDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5612..6088)
                     /gene="ybeY"
                     /locus_tag="IV247_002327"
     CDS             complement(5612..6088)
                     /gene="ybeY"
                     /locus_tag="IV247_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAP8778380.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6105..8297)
                     /locus_tag="IV247_002328"
     CDS             complement(6105..8297)
                     /locus_tag="IV247_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAP8778381.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8321..9292)
                     /locus_tag="IV247_002329"
     CDS             complement(8321..9292)
                     /locus_tag="IV247_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAP8778382.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            9997..11376
                     /gene="brnQ"
                     /locus_tag="IV247_002330"
     CDS             9997..11376
                     /gene="brnQ"
                     /locus_tag="IV247_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP8778383.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
BASE COUNT         3092 a         2420 c         2110 g         3870 t
ORIGIN      
        1 ttgatctaaa cttgctagtg acaaagccat ggcagcaatt tgacttaatt gcgttaatcg
       61 gttcttacgt actgctggtt cttctgccat gaccatcgtc tgatcaaaat aattttcgat
      121 cagtgggcga aggcttacaa gctgatcata attttcgttg atcgtttggt cagcaaagct
      181 tgttttcatt gcctctactg ctttgtgcag ttctttctcc gcttcgtttt cgaacagttc
      241 agggtctact tctgattgtt tttcaagctc cgcttttttc gctaagttca cgacacgtgt
      301 caaagcttcc atcgatggtt tgaagtcttc atctgcaaga tgctgcttga acaattgtgc
      361 tgctgcaaat acttttgtca gatctttttg ttctgcgttc aaaacagctt cgataacatc
      421 atgacggata ttcttcgttg tcaataattg gcgcagacgg cctttgataa agtcgatcac
      481 ctgttgttgt cccgctgaca agccaatgcc aaaacgatcc gtatctttac tgataacttc
      541 actaatcgtt tcttgcagaa cagacaaagg aaaggcccat tttttcgatt cgatgattcg
      601 aacgacacca tatgcttgac ggcgcaaagc atatggatcg tttgagccac ttgggatcat
      661 cccaactgca aagaaagaaa agatactatc taatttatca gcaatcgcta aaacagaacc
      721 tactgtagtc tcaggtagat ctccttcact ggatataggc atgtaatgtt cacggattgc
      781 tgttgctacg gctggtttct cgcctttaag cagagcatat ttttctccca taatcccttg
      841 tagttcaggg aattctccta ccatgttggt tactagatca aatttataga tttcagcagc
      901 gcgttgcaga tccatcaatt cttcctcaga tagtcctact tgttcgccta tgatgcgagc
      961 gatgacttgt acccgttgca ttttctcata gatgctaccg attttttcat ggaaagtaac
     1021 agtttttaat ttttcaacgt attggtcgat tgaagctttc ttgtcttcat tataaaagaa
     1081 ttccgcatct tctaaacgag cagtcaatac tttttgattg cctcgtacca cattttccaa
     1141 ttttacccga tcgccattac gagcagaaat aaaatgagga agcaacatac cttgatcgtt
     1201 ccgtacttcg aaataacgtt gatgttcttt catagaagtg actagtactt cttcaggaac
     1261 agaaagatat ttttcctcga agtctccaga aaagacagtt ggatattcga ctagattgtt
     1321 gacttcttct agtaaatctt catctagatc aatgatccag tgattttttt ctgctaattc
     1381 gtttgcttgt gtaacgatca tttcttgtct tttttgcggt gaagcaataa caaattgttc
     1441 atttaatttt tcttcgtatt cttttgcatg cgcaaatgtt acatcttcgc ctaagaaacg
     1501 atgacctctt gaagctctgt ctgttttgac atccaagatc tcaaacggga ttatttcgtc
     1561 atctaacaat gccacgatcc agtgaatagg gcggatatat tcaaaatcgt attctgccca
     1621 atgcatcgtt actgggaaag tgaggctttt aatgacatct gctaaacctg tcaatacttc
     1681 ttttgcttct ttgccgtgag tgtatttcgt tacatagaca tattccacgc cttttaattc
     1741 tctaaaagtg atagcatctg tcgtcagacc ttgtccgcgg acaaatcctt cagctgcttt
     1801 cgtccagttt ccatctgcgt ccaaagcaat ttttttggct ggtcctttga cttcttcttc
     1861 gctgtccgct tgtcgttctg gaatatttgt tactttgatt gctaaacggc gcggtgtgga
     1921 aaatgtttcg atcgtatcat aagtcaattg gttttcatct aaaaattttg ctgttttttc
     1981 ttctaattgt ttcatgcttg gtgtgactac atgagcaggc atttcttcca gcccgatttc
     2041 aagtaataag tcttttgcca tcttattcag cctccttcac gttttctgga tttgctgtgt
     2101 ttttattcaa taaagggaat cctaattttt cgcgttctgc cacaaagatt ttggctacag
     2161 cacgagccat gttgcggata cgggaaagat aacctgctcg ttctgttaca gatactgcac
     2221 cgcgagcatc taataaattg aatgtatggc tgcatttcaa aatataatca tacgctggat
     2281 gaacaagatt ttcatcgata cagcgtttag cttctttttc aaaacggtca aaattgctta
     2341 gtagtaagtc ttggtcgctt acttcaaaag aatatttgga atgttcatat tctggttgtt
     2401 tgaagatctc gccatatttc acgccatcag cccattctaa atcatagaca ctttcaactt
     2461 cttggatata agaagcaaga cgttctaatc cataagtgat ctcagaagta actggtttac
     2521 aaggtagtcc tccgacttgc tggaagtacg tgaattgtgt gatctccatc ccatcaagcc
     2581 atacttccca gcccacaccg gcacaaccca tcgatgggtt ctcccagttg tcttcaacaa
     2641 aacgaatatc gtgttctaat ggatcaattc ctaaaaggcg caagctttct aaataaagct
     2701 cttggatatt ttcaggtgaa ggtttcatta cgacttgaaa ttgatgatgc tgatacaaac
     2761 ggtttgggtt ttctccataa cgcccgtcag ctggtcggcg ggatggctct acataagcag
     2821 cattccatgg ttccggtccg attgcccgta agaatgtata tgggctcatc gtacctgctc
     2881 ctttttctgt atcataagct tgcatcagca tgcagccgtt tgatgaccaa aatttttgta
     2941 atgttaaaat catatcttgg acggtcaatt ttttactcat ctgttttgcc cctttcaatt
     3001 tttaagcgtt gctgtcaata agaatacagg aaagaaaacg acaaaaagtc cctatgtctg
     3061 attacagaca tagggacgaa taatttcgcg gttccaccct acttccgaat aaagattcgg
     3121 cagcttcatt tatcgtactc gcaagtgcct cttcaaaaat cgtttgggtc cggcttccac
     3181 tctcccgaac tcgctaaacc acgtgttttt tactgatctt ggtcaatgta cgtattcatt
     3241 ttgcagtttt cttgtttgta taatagatcc tctcagtgaa cgatctattt aatctacggt
     3301 taataataga cggaagtccc tgtatttgtc aaatagtttt gatttttttg ttgtatcttt
     3361 catttattct tctttttcag gtatctcttt ttcaggcata tttttctctg gcgtctttaa
     3421 tacgtgtgcc caagacttca tttgatcaat gaactttttg ctttttagat gaatacctac
     3481 atattcatca tacaactggt caatgaggtc acggattgcc tgcttcgttt cttctttcaa
     3541 gtggattcca ctgatcttat cataagaaat agccgaaaac attcgaacga aataaacagc
     3601 gcgaggatct gcatgataac ggtgttcgtc cattggccag tgtttctcgc atatcacacc
     3661 gccatatttg gaagaatagt caaattttcc ttttgtttcc ccgcatacga cacagtgcgt
     3721 ccaaattggc gcgattccga agcgttgcag tatttgaact tcaaagatat tcgtgatgat
     3781 ttctgcatca tctccttggt ccatccgctg cagagcttgt tgcgtaaatt ggaataaatg
     3841 cgggtcatat tgatgatctt cgattgctgc atctactaga tttagcaaat acgtaccata
     3901 accgctcaaa aagatatctt cttggatgtt ccgaaacgca tggacgtctt tgacactgct
     3961 taaaaacgac aaaccttcac ttcgaaaatt accgatatag actgcttcgg taaaggggag
     4021 caaagccgga gctaatggct gattttgccg atggattcct ttgatataaa acatcatttt
     4081 gccagaagat tccgtaaaga ttttgactaa tttatctttt tctttgtaat tacggctaga
     4141 taaaatcaag cctttcgatt ctgcaagctg gctcatattc tccctcctaa aaaataaatc
     4201 tggaaaagca gattctgccg atccagatta tttatgttac gatcttccgc tgtttttctc
     4261 aacgtacgga tttttatcca gtttatttct atacctaacc tataagaagt ccagaatatc
     4321 agtactcgtc ttttcggtag ccgaagtctt gcagatagac ttttttgtct cgccagtctt
     4381 tttggacttt gacccaaagt tctagaaata ctttgtctcc taaaagattt tcgatatcct
     4441 ttcttgcttt cgttcctact tgcttcagca tttttccgcc tttgccgata atgattcctt
     4501 tttggctatc tctttcgacg atgatcgtgg cctggatatg gattttatca ttctcgtttc
     4561 gcttcatcga atcaacaacg actgcgacag aatgaggcac ttcgtctcgt gttaaaaaca
     4621 agactttctc acggatcaat tcagaaacga tgaagtattc aggatgatca gtcacttgat
     4681 catctggaaa atattgtggg ccctctggca tttcactgac taatacatcc attaagcgtt
     4741 cgacattgtt tccttctgta gcagagatcg gaatcacttg tgcaaaaggc atctgcgtcg
     4801 aataatcttc gatgattccc agcaactcgt ctggatggat cttatcgatt ttattgataa
     4861 tcagataaac tggagaatgc acgtttttta gacgttcaat aataaaatcg tctccccggc
     4921 cgcgtttttg atcagcactg atcataaata agactgcatc cacttctttc aacgcactgt
     4981 aagctgcttc taccataaaa tctcctagac gatgctttgg tttatggatt cctggtgtat
     5041 cgataaaaat cagctgagct tctggtgtag tatatactcc ttggatttta tttctagttg
     5101 tctgtgcctt gtcactcata atggcaattt tttgacccac gattcggttc aataatgtgg
     5161 attttcctac attcgggcgg ccgacgattg cgacaaaccc tgatttatgt tctggcatat
     5221 acgttctcct ttttaattaa acaacaaatg atatatttta gggacaaata aaatcaaccc
     5281 aatgatgaca gcaaaacaag cggtcactaa aactgctcca gctgccatat ctttgatttt
     5341 tttcccaatc ggatgaaaat gaaaatccgt aaacatgtcc actacatttt caaaaacagt
     5401 attgatgatc tctactatcc aaacgagaaa aacagctaaa agcagccata accactctat
     5461 ccgatcaagc tggaaaatca cgcccataaa taaagcaagg ataccgaacg caacatgttt
     5521 cctcatattg cgttcttcct caaaaacggt tttgactcct tggacagcaa attccacgga
     5581 catcaggaaa tgtttgtttt tctctacgtt tttatctttt gagtccatag gcatccaaga
     5641 tctctttctg taaaccaaac atttctttct catcttctgg tgtcatatgg tcatacccgt
     5701 tgatatgcaa gaatccatgt accgctaaaa agccaagttc ccgatcatat gaatggccgt
     5761 aagcttctgc ttgttcagca gcacgttcaa tagagatcat gatgtctcct agtacacgag
     5821 gcaatggatt ctctccatct tcaaaaatca ctgggatttc gtcttcgcct tcttcttcca
     5881 gagcaaaact gatgacgtct gtcggtgcat cttttccacg gtaatcacga ttgattactt
     5941 ggattgccgc attgtccatg aaagtaacag acatttctgt ttcctctggc agctttaaat
     6001 aagatgctgc aaattgcagc aagtcatcga tttctttcat tttctcttct gagacttggt
     6061 ttgtttcatc cataaaggta atatccatta tacatcgctt ctttctattc ttcttgttct
     6121 tcctttatct tctcaacttc tgttttcatt ttaggatatt tgatccgaga atggaaggtg
     6181 ctacttaaac cgctgatcaa tgatttttcc acttttcgga tttctttcaa ggtcagtcca
     6241 ctatcgtcta attgaccatc ggaaatacgc tcttcgataa gattatggac aaaactttcg
     6301 attttttcgc tcgtcggatg atccattgcc cgtactgcag cctcacaact gtcagcaata
     6361 ttgacgatcc ctgcttcacg actttgcggt tttggtccag gataacggaa ttgctcttct
     6421 gttacttccg gattgcgttc tttcgctttg atgtagaaat aacgcattag cgtggttcca
     6481 tgatgctgtt tgcaaatatc gatcaccatt tgcggcatat tataatcttc caatattttg
     6541 gctccatctg tcacatgtcc aaagataata tgtttactgt cttctggcaa taagaaattg
     6601 tgcgggtttt ccgctcctga aggcaagttc tcaacgaaaa agctagcatg tttgatcttg
     6661 ccgatatcat gataatagca agctactcga gttagcaaag aacgtccgcc gatttctgct
     6721 acagcgttag cacttagatt agctaccatc atactatgat gataagtacc tggtgcttct
     6781 tccagcaact gtttcaataa aggatgattt ggattgctga gttcatttaa tacaattaca
     6841 ctgtcatcat tgaccatcaa ttcaatatat gggtgtagac cgatcgtcag caagaatgag
     6901 aagagtgcac cagtgaaccc gcagacgagc attaaccaag tcgttgcatc accaaagctc
     6961 attccttgat agacgatcaa aatgacatcc atcatcacag gaaaaataat gacccacatg
     7021 gcagcagata atccttgttc tgagatccgt ttgcgtctga caatcgttgc catgagccca
     7081 gagaaaacat aagacatcaa gataatcgtt agaaagttcg tgccaatcga atcataaaaa
     7141 atgaaaagtg cggatactgc ttggaaaata ccagccatga tccctgctct tcgattcaag
     7201 aaaaagctta aaacaagtgg tgcaaaagca gcgggataaa acagcgggat ataagtagct
     7261 tgttccgttt ggaataattg gaaaaatttc atcagcacta tgctgactga catagctgtc
     7321 acatagaaaa gcacgtattt tgttctttct cctgcatttt ggaattgaag tgaattataa
     7381 atcagtacgc cgatttgaag caaaacagct aaaaccatgg caattagcgg aaaaatcgaa
     7441 gttgtttgat tagtcagtcc tagtaattcc aattttttca tagctgaagc atcgatttga
     7501 ttaccttcac gaacaatgat ttctccttgg aagatcatca ctggttgtac agcttctcgt
     7561 gcggattgtt tcaattcttc tgtctttttc tcattcaata cgtcattcac tacgatacct
     7621 tgatccacta agtagcgaat catttgttgt tgtgctggtg tgacattcaa atattggatt
     7681 tgctcttctg cttcttgttt aaaagcttcc aattcacttt cacgaacttg tttgctcatc
     7741 tgttcgtcaa tgagcttcag actctcatcc ctcacagtat ggatctcagt ttcagtcatt
     7801 tcaaatatcg tcgaataaaa attgttcggt agtttttgat aaaacgcaac attttctgca
     7861 ttcacatttt caaaattttt cttcagcgcg gcgatccgct cttctgtagt aggctttggg
     7921 atattttcat cttcctttgc cttatcaatc ttttcgtcat aagatttgtt gatggcatca
     7981 ttcgtcttat cgatcaattc aaatagttgc ttgatccgat tgttctggtc atctgcaaga
     8041 tctttttggt aagtgtattc tggagtgact gcttctgctg ccagttttct tttttgttcg
     8101 gtttcttccg tgttctcgat cgttttgttc gcacggatac tttcttctgc tacttggcct
     8161 tctctgaaat cattggcctt ttgtttgaca ctactaaatg taacgccgca gataatgata
     8221 aaaaacaaga acagcaggaa aggcaatgct gcctttccaa gtttttcttg tagtttctgc
     8281 agtgttttat tcaacatgtg cttatcctcc taagcaaagc ttattcttga tggagatctg
     8341 ccttttcata agctttgatg atttcggcaa ctacagggtg ccgaacaacg tcacttgctt
     8401 caaaattaac aaaatcgatt ttcttgattt gctttaatgt tcgttctgca tgaacaagac
     8461 cacttgttgt ccctcgaggc agatcaatct gactcgtatc tccattgaca atcatttttg
     8521 attgataacc aagacgtgtc aggaacattt tcatctgagc aactgtcgtg ttctgtgctt
     8581 catccaaaat aacgaatgca tcatccaatg tccgcccccg catgtaagct aaaggagcaa
     8641 tttcgatcac gcctcgttcc atcaatcggt tcgtatgatc taatccaaaa atttggtata
     8701 aagcatcata aacaggacgc agataaggat ctactttttc ttttagatcc ccgggaagaa
     8761 agcctagatt ttcccctgct tccacagcag gccgcgtcaa aatgattttt tgcacttcac
     8821 cttttttcaa ggcagcgatt gctaatacta ctgcaagaaa agtttttcct gtcccggctg
     8881 gtcctacgcc aaatacgata tcatgtttgg ctaccgattc aacatatttc ttttggccgc
     8941 tgttttttac ccgtataggt tttccattac ggtctttgac gatctcttcc tcatacatct
     9001 caacaaaata atctaatgtg cctttttgcc ccattttcag tgccgtcacg acatctggtg
     9061 tacttacatg gattcctcgt ttgatcaatt cctgcaatgt ccgaatgact gaggcagcca
     9121 aggaaatctg ggattgttca ccaatgatct gaatgatttc tccacgtgta tggatggttg
     9181 tttctgttgt ttcttcaatc agttttatat gcttgtcgtg tgcacctaaa agcatgctga
     9241 catcatcagc agccgtcaat ctaatctcta aagaactaga ctcttccgtc aaaaatagac
     9301 atcccctttt tttcgattca ttctttccta ttttaccata ctcagaagaa actatatagc
     9361 aaataagaaa aggttctaaa agagttgctt aaccataagt accaagaaac gttaaaaaat
     9421 agctgcttcc attctctaaa ggtctcgttt gaataaaaaa agctgatcat gtaagctatg
     9481 cataagatag aggacaagta ttccgtctaa cttagcaagt attctctggg tatttttctt
     9541 gtttcaaaaa aagaaaacaa aatatttgaa atattcttgc atttcaaaaa aaacaggtgt
     9601 attataacgt caacaaatta atatacagca aagcgtcgag gaaaacagta ggctattcag
     9661 cgatttattc ccagtgagtc atgtaagtga gaaatgacaa taaatcgaat agacgaacat
     9721 catttccgag tcaatgtcga aaacgtagat cattgtggct gcctccatta tcaggctagc
     9781 ggataacttt atcgaaaagc gtccgtgaat gaagaagctt ttttaagcga aaaaaggtgg
     9841 taccgttcta gaatctagag cccttttccc aagtggaaaa gggctttttt tatattttaa
     9901 ggtggtggtt ttcatggaat ctgatattag tggtatttct agtttctgca acgtttattt
     9961 tgttactcaa aatgataatt attggaggaa aaatttatgg aagaaacaaa aacttattca
    10021 tggaagcagt tattgatggt cagttcactt atcttcggga tgttcttcgg gtcaggtaat
    10081 ctgatctttc ctgtccactt aggtcaaatg gctggatcaa actggatcag cgctggtgta
    10141 ggatttgcca tctcaggcgc tcttttccca ttattagcta tcttagctgt agtagtgaca
    10201 aaaagtgatg gtctgtatga cttagctaaa cctgtcggaa aatggtatgc tgccttgttc
    10261 ttgatccttg ttcatttaac gatcggacca ttctttggta caccgcggac agcagcaact
    10321 gcttttgaaa tggctgctaa accctttttg cctgaaaaat atgattcatt aggtatgttt
    10381 ttattctctg ctcttttctt tttacttgct tatttcctta ctgtacatcc aacacgtttg
    10441 gtcaaatatg tcggaaagta tttaaactcc ttgtttcttg cattattagc tatcattttt
    10501 gtcgtttctt tattccaacc catggggaat ctaaaccaat ctgctgcaga ttcttatcag
    10561 acgaacgctt tgacaaatgg cgttttagaa ggatacaaca cggttgatgc agtagcttta
    10621 cttgctttaa gtgtcacttt tgtccatgca gttcgtgatc ttggttttaa agataaaaaa
    10681 gtcacagaat taacagcaaa agctggtagc ctgagcgttg cgttagaagt catgatttat
    10741 tttggtctgg tccttctcgg tgcgatgagt ttgaataaga tgtctttatc agaaaatggc
    10801 ggaactgcac tttctcagat cactaatttt tatttaggta acttcggtgc tagcttctta
    10861 ggggtaatgg tcacattagg tgtcttcact actgctatgg gactcgttgt ttcttttgca
    10921 caagatttcc ataaactgtt ccctaaagtc agttatatga catggctgcg gttaacgact
    10981 tttgtctctt ttgtcgttgc gaatgccgga ttggataata tcatccaatg gtctttacca
    11041 gtattgatgc ttttgtatcc attgtcactt gcgttaatct tactttcctt aacagcaaaa
    11101 ttcttccaaa agagtccatt tgtctatcaa gtaacgatgt tcttttctgc cgttccagcc
    11161 gtattggata tgttggcaaa ttcgcctgca ttagtgagtc agcaaactgt cgtagcaagc
    11221 ttactaggat tttatcatca ttatgttcct tttgctgcat taggtcttgg ctgggtcgtc
    11281 ccaacgttgc ttggttacgc tggcagcttg ctcttttatt atgtatatcg cgtatcagga
    11341 tataaacaag aagcaagtga gttaccagaa gaataaccaa atcaaacaca atttaacata
    11401 agagatatta aacaggaaaa aaaacagcta gaagaaagaa taagattctt tcttctagct
    11461 gttttttagt tcttttttta cgaacacaaa aa
//
LOCUS       DACDIG010000079        11415 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.079, whole genome shotgun sequence.
ACCESSION   DACDIG010000079 DACDIG010000000
VERSION     DACDIG010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.079"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            578..847
                     /gene="rpsN"
                     /locus_tag="IV247_002331"
     CDS             578..847
                     /gene="rpsN"
                     /locus_tag="IV247_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP8778384.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            862..1041
                     /gene="rpmF"
                     /locus_tag="IV247_002332"
     CDS             862..1041
                     /gene="rpmF"
                     /locus_tag="IV247_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP8778385.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            1073..1450
                     /locus_tag="IV247_002333"
     CDS             1073..1450
                     /locus_tag="IV247_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778386.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1471..1620
                     /gene="rpmG"
                     /locus_tag="IV247_002334"
     CDS             1471..1620
                     /gene="rpmG"
                     /locus_tag="IV247_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP8778387.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1644..2627
                     /locus_tag="IV247_002335"
     CDS             1644..2627
                     /locus_tag="IV247_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP8778388.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2657..2779
                     /locus_tag="IV247_002336"
     CDS             2657..2779
                     /locus_tag="IV247_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP8778389.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2797..4320
                     /locus_tag="IV247_002337"
     CDS             2797..4320
                     /locus_tag="IV247_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP8778390.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            4344..4583
                     /locus_tag="IV247_002338"
     CDS             4344..4583
                     /locus_tag="IV247_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778391.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4776..5237)
                     /locus_tag="IV247_002339"
     CDS             complement(4776..5237)
                     /locus_tag="IV247_002339"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8778392.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5432..5650)
                     /locus_tag="IV247_002340"
     CDS             complement(5432..5650)
                     /locus_tag="IV247_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8778393.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5768..7384
                     /locus_tag="IV247_002341"
     CDS             5768..7384
                     /locus_tag="IV247_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP8778394.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7625..8965)
                     /locus_tag="IV247_002342"
                     /pseudo
     CDS             complement(7625..8965)
                     /locus_tag="IV247_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9241..9396)
                     /locus_tag="IV247_002343"
     CDS             complement(9241..9396)
                     /locus_tag="IV247_002343"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP8778395.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(9692..10024)
                     /locus_tag="IV247_002344"
     CDS             complement(9692..10024)
                     /locus_tag="IV247_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8778396.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(10538..10729)
                     /locus_tag="IV247_002345"
     CDS             complement(10538..10729)
                     /locus_tag="IV247_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8778397.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            complement(10823..11209)
                     /locus_tag="IV247_002346"
     CDS             complement(10823..11209)
                     /locus_tag="IV247_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP8778398.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
BASE COUNT         3812 a         1791 c         2191 g         3621 t
ORIGIN      
        1 gaaaactaaa ctatctgaga aattcgtcta tgaaaaagaa gctcaatcac gatgatgaaa
       61 taggctttaa attaacctac aatttttggg atatagttat aattcttttt caaaaagaat
      121 aattttatta aacagcatct aattttgaat acacgaaaga atcagtatga ttgaattagt
      181 tcacttaatg tctattctta taatataagg aataatcaaa gttttaaaaa attggcgata
      241 aaccagttaa acataaaaga ttgaataaaa agttatagtt tctttcatat tgccgtttta
      301 agtagaggaa aagctactta aaacggtatt tgtgtttgat tggactaata aaagtgaatt
      361 cttatttttc agagcctaaa ctgcatattc ttttaaatga tttgattatc tctatgtacc
      421 gataaattcg ttttcaaata gttatctatt catatgagtt ttatttcatg ttatttattg
      481 ttgacaagag caactcttgt tggtaaagta tcagtgtcaa aacgtaacga ttacgatttg
      541 aggtgtctaa atcaaaataa aaggagtata aaggtatatg gcaaaaaagt cgaaaattgc
      601 taaagcaaag aaacaaatgg cgatgattga aaagtatgca gataaacgtc aggaattaaa
      661 agccgcagga gatcgcaccg ctctcgctaa attgccaaga gattcaaatc cgaatcgtct
      721 gaggcttcgt gatcagacgg atggtcgtcc aagagggtat atgcgaaagt tcggtatgtc
      781 ccgtattaag tttagagaac ttgcccatca agggttgatt cctggagtaa aaaaagcaag
      841 ttggtaaaag gaggaaatat catggcagta ccagcaagaa aaacatctaa ggcgaagaaa
      901 cggttaagaa gaactcatca aactgtagta aaaccagaaa tttcatttga cgaagcgaat
      961 ggtgattata gacgtagcca tcatgtatct ttaaaagggt actataatgg aaagaaagta
     1021 attaaaggag cttcaaaata gtttaatata taacttttag gagggaatca aaatggaaat
     1081 tatttaccca cctttagttg aagaaggatt aaagtattat cttgaaacga cccagcaatc
     1141 gctagataaa tgcacttttt atcgttcgat ggtagagaga ggaattatta ctgaaacggg
     1201 tttaccgacc caacaggcaa ttgaaaatgg attagtcaaa gattactatg aggaccaagg
     1261 cttatcattt gatgattttt taagaatcta tccaattttt gaagagtatg acgaggagtt
     1321 atttcaatgt atcgatggtt attgggagat acctgttgat atgaaagaaa atttagtttc
     1381 acaattggaa tctggcgagt taacttttga agatgcacaa caaattcaag catatcttga
     1441 agatcgataa gtattgaagg aggaagattt atgcgtcaaa atattatttt ggaatgtgtc
     1501 gaaactggag aacgtctgta tctcaccagc aaaaataaac ggaataaccc tgaacggtta
     1561 gaattaaaaa agtattcgcc aaaattgcgc agacgggcaa tttttaaaga agtgaaatag
     1621 ctaagtggta aaaaggtgac aaaatgggca taccagttac aatcgtttct gggtttttag
     1681 gggctggaaa aacaacgcta atcaatcaag ccttgcaagt ctcaccctac cccagggaag
     1741 aaattattat aattgaaaat gagtttggtg aagttgggat tgatcacaaa ttattagttc
     1801 atagcgagga acgggtgtta caactaaata atggatgtat gtgttgcagt cttcgcagtg
     1861 atttattagc aatattgatt tctttactgg aaacaataga agaacatcaa caaccaattt
     1921 cacaaatcat cattgaaact acaggtatag cagatcccca accaatcatt caaacacttt
     1981 taacagcgcc tcaactaaga ggaagagtat atatcgatag tttattgact gttattgata
     2041 gtgacagttt tgaacttatt gaaagagaac cacaagccct taagcaactt gtcatggcag
     2101 atcgcatttt tgtgtctaga aaacaaaact tgaatttatc tgcactaaaa aaattacaga
     2161 gagaaatcaa aagtattaac cctttatccg atattcgaat gttcacacat agtgaaacga
     2221 ttcaaaaaaa ggatttttat aatttgaaaa aatttaatca tcccttagtt tttgaaagtg
     2281 attcatcaga aaatcactat aatggcaaat actatcatca acaccatcaa gtacatgagt
     2341 ttaaatcaat tctcttaact tcagatcaag acataaaaaa agatttttta ctccaatgga
     2401 ttgactggct gattttaacc aaccaaggga gcctgtatcg ttttaaagga ttgattgctt
     2461 tacaagagca ggattttata gttgccatgc aagaggtaaa cgaacaggtt aattttcaat
     2521 acaccactcg acaaaaagac caaaccacta ttattctaat cggaaaaatg ttggatgaag
     2581 aaaggatcaa acagagtttt gataaattac tggcggaaag caaataatga taagtagtga
     2641 ttgggggtaa tttctaatgg agaaaacgga tatatctagt gcatatcgac gacttaaaag
     2701 cccgaatatt aaaactagaa aaagggcatt aaaaattatc aaagaacata aacgaaataa
     2761 aatgaagaaa ttagcataat taaggaagga aaaagaatga agaaaatcag tgtaggactt
     2821 attgggatag cagctctagg attattggga gcttgctcca gcactaatga tgcaaaagta
     2881 tcgaatgata aagatggaaa attagaaatt gtaacaacat tctatccaat gtatgatttc
     2941 accaaaaaca tcgtcggcga tgaagcgaac gtggatttaa tggttccagc gggatcagaa
     3001 cctcatgact atgagccctc agctaaggat atggcaaaag ctcatgatgc tgatgttttt
     3061 gtatatcata acgaaaacat ggaatcatgg gtaccaaaag ctaaagaaag ttggaagaaa
     3121 gctgggccta atgtagtaga aggaacaaaa gacatgattt tattgccagg tagtgaagaa
     3181 gaagatcatg atcacggaga agaagatcac catcatgaac tagatcctca tacgtgggtt
     3241 tcacccaaaa tggcaattaa ggaagtttca aatatcaagg atcagttagt taaattgtat
     3301 ccgaaaaaag caaaagtatt tgagacaaat gctgaaaagt atttgactaa actgaaacgg
     3361 ttggatgctg actataccac tagtcttaaa gaagcgaaac aaaaaagttt tgtgacgcaa
     3421 cacgcagcct ttggatattt agctttggat tatggattga aacaagttcc tattgctgga
     3481 ctaagtcctg aagaagagcc atcttctggt cgattagctg aattaaaaga gtatgttaaa
     3541 aaaaataaga tcaattatat ttactttgaa aagaatgcta atgataaaat tgcaaaaact
     3601 ttggccaacg aagctgggat aaaattggaa gtactgaatc ctcttgaaag tcttacaaaa
     3661 gaacaaatgg ataatggtga agattatgtt tcagtaatgg aagacaatct aaaagcgtta
     3721 gaaaaaacaa ctatggttgc tggtgaagag gtagttccag aaaaagaagc gaaggatgaa
     3781 aagacagttg ctaatgggta ctttaaagat gccgatgtga aggatcgcga attgagtgat
     3841 tatacaggcg aatggcaatc tgtttatccg ttgttaaaag atggaatttt ggatgaagtt
     3901 ttcgattaca aagcaaaaat caataaagac atgacagctg ccgagtacaa agattattat
     3961 actactggct ataagacaga tattgatact atcaacatta aagacaacac cattgatttt
     4021 gtagtcaacg gtgaacatca tcaatacaca tacaaatatg ttggatacaa aattttgaat
     4081 tatgaaaaag gaaaccgtgg tgttcgtttt aattttgaaa ctgatgatgc aggtgctggt
     4141 cgttttaaat atattcaatt tagtgatcat ggaattgcac caagtaaggc agagcatttc
     4201 catattttct ttggtggaga aagtcaagaa aaactctata atgaattgca taattggcct
     4261 actttctatc cagcttcatt gtctaaacat gaaattgctc aagaaatgat ggctcattaa
     4321 aattcaattt ttggaggaaa tacatggtaa gaaaagtatt attgtctatg gattccaaga
     4381 atattgatgc ttatcggcgt tatcgatttg caaagcaaat gagattaaat gggagtacat
     4441 tagctgatat tgctgcaact ttacatgtta gtattgacac agttcataga gtattaaaac
     4501 gggatcctga ttgttgtttt atcaaggtca atactggaag aagtagacgt ttagcaaaac
     4561 ggcataatag cttggccaat taattgataa taccagagta gatcattttt cccttatttg
     4621 gatttgatct gctttttatt atgtcttgaa ttgtcaaatc aagcgcaaca ctttatcaaa
     4681 gaagacttca taaggtgttc tccaatctaa acactttcta ggtctgttat tcagtttttc
     4741 aatcatctga tttatcgttg agttatctac cgtagctatc tctttcgatt ttggcaagta
     4801 ttctcgaatg agtctattcg tattttaatt tgttcattgt tgccaagggc atgaggatct
     4861 ggaaaataaa agggcatatt attcaatgct tctgtaactg cgaaatgatt ggagaattct
     4921 tttcctctat ctggagtgat cgttttgacc gcctcatttg gaatactgct taaaagttca
     4981 atcattttct cgctcacata agcggctttc tttctgggga ccttctttcc tgctaaaagg
     5041 aagcatgatt ttctgtctgt caaagtcact aaacaagtac caccagtttt gccagctaca
     5101 atatcggctt tccaatgacc aaaatcactt ctatcatctg cggattttgg acggccatgg
     5161 atcgaatgag taaggcaaat ctttccacga gactcgacat gattttttgt atgtcttgtt
     5221 tttcctcgat ggtgcaatga acggatcagg cctttattgc cgtgtgagag gggttctgta
     5281 tcgaaaagat cattgcaaat tgcacgttag atcgtgataa aactaattga aaagtccata
     5341 tgttcatgct tcaaacgatt cgagatttcc tctggcgacc actgaaaatc gacaaataaa
     5401 tgacgaacaa ttgaccaaac tcacggatca tttagaatgt gataacgtcc gcattttttt
     5461 cttcgataag cataattttt ttccgctttg gaaggtgaat agtttttttc ttgtttcttc
     5521 tcagttcatg taaaatagta aacgggctat gctgaatgtc gttgccaatt atactaatag
     5581 aaaatccttc ttcgtgaagc agaaagattc tttctcgttc gagtatagta agatgacgat
     5641 actggctcat aattattttc cttctaggag tgttctcgac aaacttatac tagttggata
     5701 tcgattatga ctcattttta tgtgttgcac ttaaagtgta aattcaaggt atagaaagag
     5761 gttccctatg aaaaaaatac ctattacaat cttaactggc tatcttggtt caggaaaaac
     5821 gatacttcta aatcatattc tgtacggtga tcacggaaag gaaattgctg tcattgtaaa
     5881 tgacataggg gaagtaaatg ttgatggggt attagtcgac aagaaaggtt ttaaaagaac
     5941 tggagaaaaa cttgtttcga tgccgaatgg atgcatttgt tgtacattac gcgaagattt
     6001 gattgttgaa ctagatcaat tagtgcaatt ttctgacttg aatcaaattg ttattgaagc
     6061 ttcaggaatc agcaaaccaa ttccaattat tcaagcaatt atattagcta aaaacccttt
     6121 agacattgat ttgacccaac gattagagat tggtgccatt attatagtag tggatgtaaa
     6181 acgtttttgg gataatttcg aaacgggtga aaagattcag gaaagaaaag ctgaaggtaa
     6241 tgtagttgaa gaagaacagg atattcgtgg cttgttaatt gatcaattcg agtgttgtaa
     6301 catattactt ttaaataata gtgacttagt gtcaaccaag acgatttaca aaattaacgg
     6361 tctaattgct aagttacagt ccaatgccaa aataattagg acaattaaag gtgaagtaga
     6421 cttatcgaaa attctaaata caaatctttt taatatagat gagacttcct attcagtggg
     6481 ctggttcaaa gagttagagc ttggctatca aaatcatatt cctgaaacag aagaatatgg
     6541 tatctcaagt tttatctatc gaaatcggtt actccttgac gcacaaaaat ttgcccatct
     6601 tacggataaa aattttcctg gcaatattac aagagcaaaa gggtttcttt ggtttgccca
     6661 acatagtgat ttttcgacga tctttgaaac atcgggtagc tataaaagta ttgaatcatt
     6721 aaattattgg gtagctagtt tacctaaaat tgagtgtcaa atgattatac aaaaaaatcc
     6781 cgaattgaag gaagcctggc atccgttgtt tggtgaacgc attaataaac tagtttttat
     6841 tggtatgaag atggatcaac aatgtattaa ggaacaactt gagggttgtt taactgattc
     6901 tcttaatgca gataatttcc gaaaaatttc accatttcca tggaaaaagt ctaatatgtt
     6961 tagccagata tcgttaatga aatttttaag aaaagctggt gtcaagatag gcgatgtcat
     7021 aattattggc attttgatta ttacctcttt tattccctta tttgtattct ctatagaaaa
     7081 ggagaatgaa acaactgaag atgctatcaa atatgccatc gttcgaattg atggcaagga
     7141 aatggatcgc ttcgatttgg ataagatcaa tcataaactc gtgaattatc atcctgcgaa
     7201 gggacaatat aatattgttg agattaaaga tggaagaatt cgagttaaag aagataatag
     7261 tcctgatcaa atcgcggtta gaactggatg gatctcaaaa cccggtcaaa cgagtatttg
     7321 tttaccccat aagttagtta ttagtattga gaaaaaagaa agcacagatt actacattta
     7381 ctaatctgga aatagatgag gaggttttta ataagaaaaa gatattatta ttaataatag
     7441 caggggcttt attaagtgtg ttaagcgctt gtggacaaga aaaagaagcg gtgacaacag
     7501 gcacaagtat ctttaacggg acctgcaatg gtttagttaa cagtcggtta gattacttat
     7561 tttggctctt cgtcaatgag tgttggtgaa gaaattcaat agaataatcg agatactcac
     7621 acggctagac agctatcttg tctagccgtg ttgttgttct ggctttagtc agttgggtag
     7681 tattcttagt tttcttaaaa tattttcctc gatataaaaa gattcgttct cggtagtttt
     7741 gaaagtttct atagccatag gcaattcgtt ttagtacttt gatatggtta ttcaaacatt
     7801 ctatagggcc gttggaatag ggaacgcgca acgctcgttt gatttctgtt tggtatttct
     7861 tgaaggtcgt gaacactggt tggtagtgct ctggcaactc cttgtagtct tcttttagga
     7921 gcgcgacgaa ttcaggaaca tcttgtcttc tgataacgga aagaaaatcc tggtagactt
     7981 cgtagcctcg ctttaaggaa tcatcataag ataatagccg atcgactatc tctgcttctg
     8041 tcagatgatc tcgaaatgac ggacgccagt agcgatgctg acctttcaat tcccacgcat
     8101 tcttttgaag tagtttccag tatttcttta agtgcttcga ccgacgatcc ccttttttaa
     8161 aggtcttcat tacttgaatt cggtggttgg taaaagcccg accaatgtgt tggctaatat
     8221 gaaagcgatc ggcgacgatt cgggcattcg gaaaacactc tttcaccaat gtgcgatagg
     8281 gtgtatagaa atctgaaacc actagacgca cttgttcacg gacttttata gggtagcgca
     8341 agaaatagtc gcgtaaagga ttgagttgcc gattttccac aatatcaatc agttgatggg
     8401 tcttgccgtc catcatgaca aagctcatgg ctcccgagac atttttcact gatttgaatt
     8461 catcgaaaca caccacttct ggtaacagct ggctcaaaga aatggtttta ggccgaaggc
     8521 tgcgaagtat tcgaatgaca gtggtcggag aaatgtgctt catacgtgcg atcaaagaca
     8581 tagaaagagg ttcgcttaat agctcaagaa tggcttgttt gactctgcga gcaatggagc
     8641 agcgacgatc actaactgaa tcttctgcta aaaaagtctt tccacagtgt ttacatttga
     8701 agcgctgttt gttaatccgc agaatggtct tgtagttact gacatcattc aataagatgg
     8761 atactttttt ccagccctat ttaatgatcg tttgttcatt ttgatagtgg cgatgaaagc
     8821 attcctccgg ttgataggtc aatgtgccat caaaaacaaa acatgtttcg tcattgattc
     8881 gctctttgcg aaaggcatct tcatgaaaga taagatttag gtctagaata tctagtaact
     8941 ctttggtaaa ctctgaatag gacatcttga aacactcctt tgaatggttt tcgtcgactt
     9001 tattctaaag gattttaagg tgtctgtttg cgtttattag aaaaaagaaa aagtattggc
     9061 aacagaaggc gtcaccaaca ctagatatta tagatccctt attttcttcg cttcaaattg
     9121 tcaaggagtc atcataaatt ttgtatggat tcttttgttt gggtagtagt cttctaaata
     9181 atcttggatt aacaccttca tctctatttt acttttatat ttgttggagt cgattaattc
     9241 tcatttgatt gatcgatgaa acgcttcaat tactgcattg tcaacaggag tcccagtttg
     9301 gctcactgaa tgtgtgatgt ggtggtcgtt caacagctct tgataaataa aagaccgatg
     9361 ctggctacct ctatcggaat gaattaccgt aggcaattct ccttaataaa tttgtaatgg
     9421 acggacgaca atctcttttt tcatgtgtga atctaccata tagtaacgta gaatacgagt
     9481 tgctaagtca atataggtgg acaaatagag gcgaccgtca ttacatggaa tgtaagtaat
     9541 atccatcacc caaacagcat caatttggtt ctgttcaaat tatcgattca acattttttt
     9601 accatttctt tgattgtttt ttctctacca tgagaagatt tgcgtcgatg aaatcctctt
     9661 gtgcataaat tcatttcacg cataagaaca gctacaacac gtttattgat ttcgattcct
     9721 tcatcgtaaa gtttttgagt gatccctgga gatccgtatc gatctttgtg ttcgtagaac
     9781 actcgtcttc ctcttttagt cgtttatttt cctgttcaag aatgtagaaa tctgctttat
     9841 caggtaatct ttttccttgt ccagcaaaag catttttccc gtatcgacga tattctgaaa
     9901 cccatcggga aattgttgga cgagagatat tgtattcttc agcaattttc ttggtagtta
     9961 cttttttctc aaggatatcc tgtgaaatct ttagtttaaa tttacgatca tatgtttttc
    10021 tcaatagagt tcattccttc cggtaacgta actgatagtc aagaaaagtg tatcatagcc
    10081 caacttagta tcagggtttg tcaactaaac tgtggaagtt gatagataag agtttttaac
    10141 aaccaaaatt ctcttgacta tttttgaaat cagacaaatt ttgattggac acatagttga
    10201 ataaatctac cgtcgttacg gaaggtaaat cgcatgcttt tcaattctag aggagaagtc
    10261 ctcgttgaag taccatagcc cagttctgga ttcgacctcc tgcccacttc atacgtaaat
    10321 aaagtaattt taggagtgag actgtgacaa aagttatttt taagctaaaa acgaattaaa
    10381 aaacgttgat aattaaaatc attcgtttta gattctatga atcattatag aacgctgtaa
    10441 aatcgatact ttataattat aaagaagata gtttcaaatt tattgttcga aatttgaaac
    10501 tatcttttta cttatgtcac agactccctt agttgagtta ttctttaatt tcgaatttcg
    10561 agatagctag atatattgca agcatacatt cttgataaca atcttgatca tattttccag
    10621 ttctccagga ttgcttatga atcaaagagc taaatctttt gagaattgcc tcaacagctt
    10681 cttcatttcc agcctttgct aaaatgaaat cttcttttaa agaattcata aatctacctt
    10741 cttcccatgg acttataatc tatttctttg ggaaggagga gaacgagcaa agaagcatac
    10801 aaaattagtt attatgcaga tattaaacca gcattttttg aatttttttg taaagtcgat
    10861 gttttaaatt tgtcgcaccc tgtcttgtaa tccccagaac ctttccaatt tctttatcag
    10921 ttttatcaaa gataaatttt tcgataagaa aaatttgttc aggttctttc aacaaaggaa
    10981 ggatttttgc taattttata tcttcaacaa ggagagtcac atttaaaaca ttaatcggtt
    11041 gtttaaattt tatagtatct ttagtaaaca aatatggtgg gagttgatca agttgttcat
    11101 gtttttgaat ttcttcttgt tttttataat aagtattagc ggtgttcaaa atgacctttt
    11161 caaagaatcg aatcaactgg gcatttacgc gttcgtcgtt atctgacata ttgcacacct
    11221 cctccattaa gatttaaagg gaaaagaggc aaaaaacacg aaaagaagct aatgcaatta
    11281 tgctgcgctg cgaataactt ctataagctc acatgcattg ataggactgc tgatgtttat
    11341 tttcaaaaat gtttttttag ttgataatgg atagttatct ttatacttag aaaagaaaat
    11401 tcatatatct tctta
//
LOCUS       DACDIG010000080        11370 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.080, whole genome shotgun sequence.
ACCESSION   DACDIG010000080 DACDIG010000000
VERSION     DACDIG010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.080"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            133..1068
                     /locus_tag="IV247_002347"
     CDS             133..1068
                     /locus_tag="IV247_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAP8778399.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            1158..2261
                     /locus_tag="IV247_002348"
     CDS             1158..2261
                     /locus_tag="IV247_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP8778400.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            2274..3227
                     /gene="menA"
                     /locus_tag="IV247_002349"
     CDS             2274..3227
                     /gene="menA"
                     /locus_tag="IV247_002349"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAP8778401.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(4162..5508)
                     /gene="gor"
                     /locus_tag="IV247_002350"
     CDS             complement(4162..5508)
                     /gene="gor"
                     /locus_tag="IV247_002350"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAP8778402.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFCGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(5642..6883)
                     /locus_tag="IV247_002351"
     CDS             complement(5642..6883)
                     /locus_tag="IV247_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP8778403.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(6901..7824)
                     /locus_tag="IV247_002352"
     CDS             complement(6901..7824)
                     /locus_tag="IV247_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP8778404.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(8045..10537)
                     /locus_tag="IV247_002353"
     CDS             complement(8045..10537)
                     /locus_tag="IV247_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP8778405.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(10544..11011)
                     /locus_tag="IV247_002354"
     CDS             complement(10544..11011)
                     /locus_tag="IV247_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAP8778406.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(11147..11220)
                     /locus_tag="IV247_002355"
     tRNA            complement(11147..11220)
                     /locus_tag="IV247_002355"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11184..11186),aa:Arg,seq:acg)
     gene            complement(11278..11351)
                     /locus_tag="IV247_002356"
     tRNA            complement(11278..11351)
                     /locus_tag="IV247_002356"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11315..11317),aa:Pro,seq:tgg)
BASE COUNT         3374 a         2325 c         1997 g         3674 t
ORIGIN      
        1 tgaaagaaaa cgtttgaaat attacatggg ttatttatca aaaatagttg acttataggc
       61 taaaaagatt agaatgtata atgaatcaca aagaaaaatt gtggactaga aatggaatgg
      121 tggggtatta aaatgaagaa aaaacgtttt gtaactggat ttgccgtatt agcattttca
      181 gctctagtgt taggcgcttg cggcgcagat gacaacggaa gctcaaactc ttcttctgaa
      241 tcatctacag cacaatcttc gactgcaaaa agcacaacag aatcatctgc aaaagttgta
      301 gctggcggag atttgcaaga cggcacatat aaattagaag aaaaaaatta ttcaaatggt
      361 taccgtgcag tctttgaaat ggttgtaaaa gacggcaaaa tcactgaatc aaaatatgat
      421 aacgtcaatg aaaacggcga atcaaaaaca aaagatgcag aatacaacaa aaacatggaa
      481 gcaaaatctg gcacaaaccc agaaaaatac attcctgagt tgaatgacca attcttaaaa
      541 gcacaaagcg caagcggtgt ggaagtcgta actggagcta ctcactcatc tgaaagcttc
      601 caaaactacg cacaacaatt gatccaagct gctcaagctg gtaacacaga tacaatcgaa
      661 atcgacaatg gtgctgattt gaaagacggc acttacaagc ttgaagaaaa gaactactct
      721 aacggctacc gtgtgcaatt cgaaatgaca gttgctggcg gcaaagtaac tgaatcaaac
      781 tttgattata tcgacaaaga cggtaaatca aaacaagatg atacagaata taatgaaaat
      841 atgaaagcaa aaagcggtac agaaccaaaa acttacatcc ctactctgaa cgacgaattc
      901 gtcaaagcaa tgggcgaaga agatggctca cctgctaatg tagaagttgt tacaggtgct
      961 acacacagct cacattcatt catcatgtac gcacaacaat tagtgaatgc agctgaaaaa
     1021 ggcgacactc aaacaatcga agtagataat atcgtaacag aaaaataatc acataatcat
     1081 actaataaaa gttggagggt gggacgatag ccagattagg cagtcgtctc acctttcaaa
     1141 atgaagagag gaaaaagatg cggaaaaaac tactattttc cttaatcaca ctgggcatct
     1201 cttttatggt gctaactggc tgcagcaact cagcaaataa aacaaccact gaaagttcag
     1261 cagctaccaa gatattgaaa gatccatatt ctgatgaaca gttcttgtta gggacttacg
     1321 ttcgtatccg tgtgtatgat gaaggcaaag aatcagcatt gaaacctgcc ttcgatcgag
     1381 taaaagaact tggtgataaa atcacgatca atcaaaaagg ttctgagatc gatgaagtga
     1441 atgagcaagc tgggatcaaa ccagtaaaag tttctgatga cgtctacacg ttagtcaagc
     1501 gggcctacga atatagccaa gattctcaag gcggattcga tatggcgatt ggtgcaatca
     1561 cacaattatg gcgaatcggt tttgatgacg ccagaaaacc ttcccaagaa gaaatcgatc
     1621 aggcattgaa attagtggat tatcataaga tcgaactaaa tgacaaagaa aaaactgttt
     1681 atctaaaaga aaaaggaatg atcatcgatt taggtgcaat cgccaaaggt tacattacgg
     1741 acgaagtagt gaaagtcttg aaaaaacaag gagtgaccac tgccatcgtt gacttgggcg
     1801 gtaatgtcta tgtcttaggc cacagcccaa gaggcgaaaa ccaagactgg acagtcggca
     1861 tccaagatcc gaacatggct cgcggatctg ttttaggttc tattaaagaa cggaacaaaa
     1921 cattagttac ctctggaatt tacgagcgtt atttagaagt agatggcaaa aaataccatc
     1981 atttgtttga tccaaaaacg ggttatccgt tcgacaatga tatcgcaagt gtcacgatca
     2041 taacagataa atcaattgat ggtgacgggt tgtctacagc agtcttttca atgggtgtga
     2101 aaaaaggact ggaatacgtc gaaagcgaat tgaataacgg gacggaagcg atcttcgtga
     2161 caaaagacga taaagtatat gtgaccgatc caatcaaaga caccttcaaa ttatcggagg
     2221 actcccatta cacgatgggt gaccgttctg aactaaaata aggagtacga accatgacac
     2281 taaaaatctt tttagaagta gttgaaatac gaacaaaggt ggctagtgta tttccttttg
     2341 cggtaggggt attattctct atcgcttttt ttcatgagat acattggcta aacacttttt
     2401 tattttttat tggaatgcta gtttttgatt tagcgactac cgctatcaat aattacatgg
     2461 attatgaaaa ggctcattct gaagtatata agtatgaaga aaatgtcatt ggccgcagaa
     2521 atctatctcc tgtattgatt cgaaacatga ttttagctat gattgcattt gtttttgtta
     2581 tcggcgttat tctaacgatc aatacgggtt ggcttttact tttgatggga tttatctgtt
     2641 gttttattgg agtattctat acattcggac cgattccttt atctcgaatg cctttagggg
     2701 aaattttcag tggggtgact atgggccttg gaatttttgc catggtcgtt tatgtcaata
     2761 cattcggttc agatgtctat gatctgacag tcaacttttc atctggacaa tttgctttgg
     2821 taggaaatct ttgggctata gcagcaatcg tattagcttc cttacccttg gtgtttacga
     2881 ttgccaatat catgttggct aataatttgc gggatcttga ccgtgatatt gaaaatcatc
     2941 gctatacact agttttttat atcggacgtc ctgcgggaac agtcttattc cagctattga
     3001 tgtatgcttg ctatttcgtg attttaatgg ggttgttgag cggtatctat gaatggccga
     3061 tcttggtcac cttcttgacc ttgccaattg tgaagaagca tttggatcaa ttcaagcaaa
     3121 cgttacccca gcctatcagt ttcagctatt ccatcaaaaa tatggtctta ttcaatgcca
     3181 gttatgtatt ggggctatta ctaacaatca ttatgcaagc catttaacag catgaagagg
     3241 ttgtgaaaaa actgccctgc atcaagtaat aagaacagcc aaataaaaat agctccttat
     3301 atttttcgtt tggttgggct tattaccgca gatgcaagct tttgaacacc gtttatcagg
     3361 aggttgtgaa aaagtcgctt agtttcgagt attaagaaaa attttgtgaa aatggctttc
     3421 cacatttttg cataaatttg gcttaatacc gagaaactgg cttttgaaca ccgtttatca
     3481 gaggttgtga aaaagtcgct tagtttcgag tattaagaaa aattttgtga aaatggcttt
     3541 ccacattttt gcataaattt ggcttaatac cgagaaactg gcttttgaac accgtttatc
     3601 agaggttgta aaatcagtgt tttgtcctga gtattagaga aacaaatgga aaaatagttc
     3661 ctcatatttt gccgtttttt gagctaatac cgaaggacag ctgattgaac actatttgtt
     3721 cggaaaaagt gactaactat gacacaattt ttttcacagt cccttttttc acttgatttg
     3781 attcgtacca gcgaacttga cgagaacttt cttggtttgg taactatatg gtattctttt
     3841 aacatcctcg tttgcttctt ctaatttatt tatacaaagt cacacttttt taaacatctg
     3901 ttcttcgata catttctatt tcttttaaac atcgatacca atattttgaa cttaagagtg
     3961 cataccaaat ggattcattt actcttcgtc agttacgaag ataatcaaaa tctccaactt
     4021 ccctacttat ttcgttttga gaattatcga agcaatctta cataaataac taaaaaacaa
     4081 aaaaccagaa aatcagtata ctgattttct ggttttacat ggtttgttca ataaaaagct
     4141 gttggaactg ttaatattaa attatcgcat cgttacgaat tcttccgctc ctgttggatg
     4201 gatggctact gtattgtcaa agtcttcttt agttgcaccc atcttgatag ccacggcaaa
     4261 accttgcagc atctcatcga caccaacacc aatagcatgt aagccgacga ttctttcttc
     4321 ttttccgaca cagatcaatt tcatttcgca tttttggcga taatcattta atgcaaagta
     4381 cattggggta aagcgtgaac gataaacttt caattcattt tcgccatatt tttccaatgc
     4441 ttcttcttct gtcaaaccaa ttgttgcaat tggtggatgc gtaaagacga ctgtcggaac
     4501 aagtttatag tcaagataag aatctttctt tccattaaat aaacgttctg ataaacgtct
     4561 accagctgcg attgcaacag gagtcagatc taatttaccg atcacatcac caacagcaaa
     4621 aatatgttca tccgttgtat tttggtattt atctactttg ataaaccctt ttttgtccaa
     4681 ttccacattt gtattttcca gacctaattt ttcggtattc ggtacacgtc caccggcaaa
     4741 aagtacacaa tcaccagtta aggtttcacc attttcaaat agcaccgtat attcgtcatt
     4801 gtttttagta atttctttag caatatgatg tgcgtaagtt tttattccag cttcttggta
     4861 catttggatc aaattgtccg aaagcatttt atcaaacgtt ctaagtggtc gttcttttcg
     4921 atatgcccac atgacttcac tacctaaacc acaaaataca cctgaaagtt cagccgcaat
     4981 gtatccagca cctaaaacga ttgctctctt aggtaactct gttaattcaa aaaatccatt
     5041 tgaatcaatt gcgtattctc caccaggaat ggtcatttta cttgcacggc ctccagtagc
     5101 gatcaagatt ttaggagcac gataggtttc accgtttact tcgatcgttt gactatcgat
     5161 aaaagtagcg taacctttta tagcctcgac atgattgcta tctaatcctc tttgataagc
     5221 accgtgtaga aaatcaatat atttttctcg tctttctact agaccggcga aatcaacatt
     5281 ttttaaatct gcttggatac cgtaacttga ggcatctcgt tcaattgttt ccaaaatcgt
     5341 acttgcttgc cacataactt ttttaggtac acaaccgacg ttgacacatg ttccgcccaa
     5401 ttcattcgct tcaatcagca aaacttttgc gccatgcatc cctgctctgt ttgctgaagc
     5461 aataccgcca gagccgccac caattacgat gtaatcatat atttgcattt tgttcctcct
     5521 tattgaagag atcaatcaaa acctttattt aacgatcctt tttctacaga aaacaagtgt
     5581 aacacttttc ttgaattttg tatgcgaaga tgcttaaaac agggatatat cagctttttc
     5641 cttatttacg ttttcggatc atatctccag gtttcaccgg ctgtgcgact ggcatagtta
     5701 aaatcatcat tggatttggt gctcggtcaa tggattcgcc ttcttcatta tgcataactt
     5761 caacggtttg actgaaatga ctgaatcctg gaccataaaa ttcgatttca tctcctacag
     5821 aaaacaagtt tctctgacgt atcgtagcaa tctgtgttgc ctcatcataa gataatactt
     5881 caccgacaaa tttatatact gggattttac gacgttcacc gaataattgt tcgttttcag
     5941 ttggtgtatt gtagtaaaaa cctgtagcta attctcgttg tgctactttc cataactcgt
     6001 cgatccattc ttgcttgcag acatagtttt caggatcttc catataactg tctacagccg
     6061 ctttatagac atttgacacc gtagatacat aatggatcga tttcattctt ccttcgatct
     6121 tgaagctgtc tacgccgttt tcaatcaatt ctggaatatg ttcgatcatt gacatatcca
     6181 ctgcactcat cgagaattct tcttccactt cgcctgtatc agttaaactg tttcgttctt
     6241 gaccaaaagg catatcgaac aatccatact tccagcgaca agattgagaa catccgccac
     6301 ggtttgcatc ccgcatcgac atatggttgg acaatgtgca tcgaccagaa taagagatac
     6361 acatggcacc atggataaat gcttcgattt cggtatccgt atttttacga atctctgcca
     6421 cttcctccat tgaaacttca cgagctaaaa cgacacgttc caacccttct tctttccaga
     6481 attccaatgt ctcaaaatta gttgctgaag cttgagtgga taaatggatc ggcagacctg
     6541 gggcttctgt cgcacaaatt tcgatcaaag ctggatcaga aacaatcact gcagaaatac
     6601 cgacatcacg caaactgcgg aaaaattccc ctgctccatc ttcatttcct tcatgggtga
     6661 ccatattagc agcaacgtat accttcgcat catgttcttt tgcaaaagca actccttcag
     6721 ccatttcttc ataagtaaaa ttacctgcac ggcttcttaa accatatgca ttcccgccaa
     6781 tatatactgc atctgctcca taatggatcg cggtttttaa cttttccagt gttcctgctg
     6841 gcgctaggat ttctggtcgt ttcaattttc tttttactgt catttctttc atcatactcc
     6901 ttatttgata gcagaaggat caatataaaa gaatcctgta tctaattcac ggttttcagg
     6961 atgcaattta ctgatttcgc tagtcatttt ttccgctttt tcttgatccc agctaccttc
     7021 taaaatttgt ttttttgctt cggcgaatag tcgagtgatg tttacgaatt tttcgcctgg
     7081 agtaaataat ccgtccaatt tccatgtaca gtaatgatgc tgataaagtt cattcaattc
     7141 attcgagaga ttcagatcat tgctggcaaa aatatgtgtg ccatgactat cttcataaat
     7201 agaataatgt gtctcttctt ttttaggttc cgagataaaa agacctcttt cacgatcttt
     7261 ttgttcgtct tgtttcgtat agttatagta attctgtagc agtggacgtt tggattgatg
     7321 gatgcatgta gcaccataca caagggtttc tacaggaata tcgagttttt cttccatcgc
     7381 cttcatctct tcaaaaggaa cttcacgagc aagtactgct ccaactgcgc cttttttcgc
     7441 ccagaaattg atttgacgtg cgctcgttac taatgtttca gcatcataaa tatacggtat
     7501 acgcaattca ggattttttt tcatcgtata aataatacct gtatcaccaa ctgtgatcac
     7561 atctacgttt atttctttta aaaaagccaa atattcaggt acatttttca ttttttcagg
     7621 atgaatgatc ccatttacag caatattcgc ttttttacca tacttgtgtg ctaattctac
     7681 caattgtcgc tgttcttctc gtgtaaatga agcaggcaga cgcaatccaa attgctcttc
     7741 accgaaatat agtgtatctg cacctgcagc aagcaactgt tctgcttgtt caatagtttc
     7801 tactgtggtt atgagttcga tcataatttt ctcctcctac aacttcataa tagtagcata
     7861 gctctaaaaa ctttccaata cttcttctat ttaaagtcgg atttttgaaa attatttcac
     7921 aaaaaatcaa aataaagttc gatatattct gtccaaatat aaaaagagat tgtgacaaaa
     7981 atcttgtcac aacctcttaa tcaagtctta ctattataaa aattcagcta cacaggccaa
     8041 gtaactatag ggaacctact gtttcctttt taggtccgcg aacattcaat gtgatcttac
     8101 cttttgaagc acctactgtt acgtggtcac ctaaacgaat ttctccgcca agcaaagcct
     8161 cgctcaatcg atcttcaatt tctttttgaa gtgcgcgacg gattggacgt gcaccatatt
     8221 caggatcaaa tccagctttc ccgatcacat cgattgcaga tggtgtgatt ttcacttgaa
     8281 tatcttgttc tttcaatcgt ttgataatcg ctttgctcat gatttggacg attgtatgga
     8341 tttcatcttt actcaatgaa tggaagacga cagtctcatc gattcgattc aagaattcag
     8401 gtcggaaagc tttctttaac tcttctaaga tacgtttttg catggctttg tgatctttag
     8461 taacatcttg gacattgaat ccgacatttt tttcttctcg aatttgtgtc gctccgatat
     8521 ttgaagtcat aatcatgatt gtatttctaa agtccacttt tctaccttta gaatctgtta
     8581 aatgaccatc atccaatact tgcagaagta aattgaaaac gtctggatgt gctttctcca
     8641 cttcatctaa gagaatcact gaatatggtt tttgacgaat tttttctgtc aactgtccac
     8701 cttcatcata ccctacgtat cctggaggag acccgattaa tcggcttgta ctgtattttt
     8761 ccataaattc tgacatatca acacggatca gggcatcttc acttccaaac atgacttctg
     8821 aaagtgcttt ggccaattct gtttttccga caccagttgg tccaaggaac atgaatgaac
     8881 caattggacg atcaggatct ttcaatccgc ttcgtgcacg gcggatcgca cgtgaaactg
     8941 ctttgacagc ctcatcttga ccaacgacac gttcatgcag aagtccttcc agttctaaca
     9001 agcgttcgct ttcttttttc tccaactgct gtagagggac accagtccac tccgatacaa
     9061 ctgtagcaac atcttcttct gttacacgat cagaataacc ttgctcctgt tttgcttcaa
     9121 ttgcagaaac tttttgcagc tttttacgta gtgctttttc ttgtctgcgt aattgagcag
     9181 cattttcaaa gtcttgtttt tggatcgctg cttccttttc ttgtactagt tcatcgattt
     9241 ctagtttgat cacagttgat tttgttaagt gatcggtttg atccaaacgg actttcgctg
     9301 ctgattcatc gatcaaatca attgctttat cgggtagttg acgagaagtg atataacgaa
     9361 cagaaagatt gactgctgct cgtacagctt catctgtgat ctccactcca tggtgctctt
     9421 catatcttgt acgtaatcct tgaagaatca cttctgcttc ttcaggagtt ggctcatcta
     9481 cttgaatgcg agcaaaacgt cgctctaatg cagaatcttt ttcgatatat ttttgatatt
     9541 catctagggt cgttgctccg atcgtttgta attctcctcg agctaacgcc ggtttcaaga
     9601 tattcgaagc atcaattgct ccttctgctc cacctgcacc aattaaggta tgcaattcat
     9661 caatgaataa gattacttga ccatcattat agatttcgtc gatgaccttt ttcattcgat
     9721 cttcaaattc accgcgatat ttcgtaccag caaccaaaga tcccatatca agcatcatca
     9781 ggcgcttttc ttgcatgtct tctggcactt cacctaaaat gattttttga gctaaccctt
     9841 cagcaatggc tgttttgcct acaccaggtt cacccaccag tacagggtta ttctttgtcc
     9901 gtcggctaag aatctggata agccgtttca cttctctcga acgaccaaca actgggtcca
     9961 gcttgttttc ttttgctaat ttcgtcaaat cacgtgctaa tgaatctagc gtaggtgttc
    10021 cttgcggtgt ttgccgttta tcttgtacat ttacatttct acgacgattc attccttttc
    10081 cagcagaatc accagctagc cccatttttt gtttcagaac ttgacgggct tttgaaaggc
    10141 tgatacctaa gttgatcatg attcgagtag ccatgatact ttcatctttt aataagctca
    10201 tcaaaatatg ttctgttcca actttttgag aaccagtttt cttcacttcg ctgctagcta
    10261 aagcaaaaac ttgtttcatt cttggtgaat atggaagaaa gactccttta gggtaagaac
    10321 ggataccacc ataaccaatc agatgttcaa tttcttcata aatttccgtt tcattcaaat
    10381 ccatttcacg taatactttt ccagcaattc cattttgctc gaccactaag gctagtaaaa
    10441 catgttctga actaactgat tgatgtttca accgttttgc ttcttcttgt gcaatttcta
    10501 atactgcttg ggctctttca gtaaaaagtt cttccatcta atctcacttc ccatcttcat
    10561 aacttaaacg ttccagaaat gctcggagaa tccgagcacg tgtatgatct tcatattcat
    10621 tgaaattcaa tgtgtttttt gcaattgcac tcaacatcaa gtttccttct ttttttgtaa
    10681 ttatttggtc ttcataaagt ttttgaataa tagcaaaagc atttttctca ctgattgtat
    10741 catcaaataa ctgatttatc tgctcaagta attgtttttt atctgaaatc cttacttttg
    10801 caattcgtat ataaccacca ccaccacgtt tgctttctac tagataacca cgttggatgg
    10861 taaaacgggt attaatcaca tagttgattt gtgaaggaac acagttaaag aggtctgcca
    10921 tttcaatcct tcgaatctcg atcatttcac tattttccag aattttcttt atatatgatt
    10981 caattaaatc cgaggtattc tgatgtgcca tcttattcat tccctttgac taatattgac
    11041 cttaattata acgaatttga taatagaaag aaagaagaac gctttttgtt aaataaatcc
    11101 tttagaatca acaaaaaaaa atacggaata tactattccg tatcgacgcg cccaagagga
    11161 gtcgaacccc taaccttttg atccgtagtc aaacactcta tccagttgag ctatgggcgc
    11221 atattcaatt taaaaaaatt ataaatcatt tgaaaacaaa aagcaagata aaaaaaatcg
    11281 ggaagacagg attcgaacct gcgacccctt ggtcccaaac caagtgctct accaagctga
    11341 gctacttccc gtttaaaaaa taaaaaagac
//
LOCUS       DACDIG010000081        11262 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.081, whole genome shotgun sequence.
ACCESSION   DACDIG010000081 DACDIG010000000
VERSION     DACDIG010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.081"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1669)
                     /locus_tag="IV247_002357"
     CDS             complement(<1..1669)
                     /locus_tag="IV247_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8778407.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTH"
     gene            complement(1629..3383)
                     /locus_tag="IV247_002358"
     CDS             complement(1629..3383)
                     /locus_tag="IV247_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8778408.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLIKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3493..4062)
                     /locus_tag="IV247_002359"
     CDS             complement(3493..4062)
                     /locus_tag="IV247_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAP8778409.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(4080..5504)
                     /locus_tag="IV247_002360"
     CDS             complement(4080..5504)
                     /locus_tag="IV247_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8778410.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDIN
                     FDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQF
                     FTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSK
                     TNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGG
                     IKKDIKLRVEDSTLIEQIFNDITK"
     gene            complement(5506..6174)
                     /locus_tag="IV247_002361"
     CDS             complement(5506..6174)
                     /locus_tag="IV247_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP8778411.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(6304..6888)
                     /locus_tag="IV247_002362"
     CDS             complement(6304..6888)
                     /locus_tag="IV247_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8778412.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(7219..7452)
                     /locus_tag="IV247_002363"
     CDS             complement(7219..7452)
                     /locus_tag="IV247_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAP8778413.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(7467..8417)
                     /locus_tag="IV247_002364"
     CDS             complement(7467..8417)
                     /locus_tag="IV247_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP8778414.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(8414..9256)
                     /locus_tag="IV247_002365"
     CDS             complement(8414..9256)
                     /locus_tag="IV247_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP8778415.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(9258..9977)
                     /locus_tag="IV247_002366"
     CDS             complement(9258..9977)
                     /locus_tag="IV247_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP8778416.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            10669..10827
                     /locus_tag="IV247_002367"
     CDS             10669..10827
                     /locus_tag="IV247_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8778417.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            10899..>11262
                     /locus_tag="IV247_002368"
     CDS             10899..>11262
                     /locus_tag="IV247_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8778418.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAM"
BASE COUNT         3857 a         2026 c         1500 g         3879 t
ORIGIN      
        1 tatgagtacc ttgatcaact agttttcctt gatccatcac tacaatttta tttacctttt
       61 caatagactt caagcgatgc gatataatta gtactgtctt atcttgtatt aaacgattta
      121 aactttcctg aatacgtttt tcattttccg catctaaact ggcagtaatt tcatctaaaa
      181 ttaaaattgg cgcatttttc aaaaaagccc gtgctatgga taggcgttgt cgctctcctc
      241 cagaaagtga acttccgttt tctccgacca atgtatgata accgtcaggt agtttttcaa
      301 taaagtcttc gcagttagct aaacgagctg cttttttcac ttcctcatct gacgcttgac
      361 tatttccaat acgtatattt tccaaaatac ttgtattaaa caaagttacc tcttgaaaaa
      421 caatagcgat tttgctaaac aaagaagctg gagatacacg tttaatatca tacccatcaa
      481 ttaaaataca gccttcatca taatcataca gtctagacac taatttcaag atactagttt
      541 tcccggaacc actagcacct actaacgcag tcacttctcc ttgtttagcc gtaaaagaga
      601 tatgatctaa aattggggta ttgttatcat atgagaaaga gacatctcgc aactcaatgt
      661 caaaagattt cagaggacta tctgatccct cttggatact ggtctccttc atagctctaa
      721 ttcgctgtat tttaggagct aaataaaata tttcaagcac tgcttctttc atagaatcga
      781 aggaatcctt tatttttatt gcggctagta agtaaccaac gacgtagagt atagtcactt
      841 cacccgttaa cagtaagtga acacctacta gaatgactac tgctaaacta acatatgaga
      901 aaatagagga taaggccatg actgaaaaag tacttaattc tacctttaaa tgaatccttt
      961 cactctcttc catttttttg aaaagtctgt cttgaacttt tttagataga ttaaagctat
     1021 taatctcttg ctgcaattct atagtttctt gaaattcttc cgagttttct ctcaaagtat
     1081 cgtaatattt agtattggct ttcgtttggg atttctttga taacaagatt aagacaaaac
     1141 taaataacgt tggcaaaata acagctaatc ccattttgac attaccaacc aaaagcatca
     1201 ctgaaataaa agggaaaaac agagccatac cgccggcttt aggtattgca tgactcatcg
     1261 catgctcaat accttcaacg tcagacatga tagtttgaga taaatctgat aaattatgtt
     1321 ttgaaaaata ggataatggt aaatttgaca aatcgtctgc aatttgcact cttaaatgcg
     1381 cactttcttt ataagtacta ttatataagt tttcatactc cctatctaac aaccaataca
     1441 tgaaaagcaa agtaacaaac gatacggcaa aataaagcca acggggcttt gtattttcta
     1501 aaactaattc gtctatcagc atcattataa tgaatgctgg taccatatta atgcagtata
     1561 caaaaaaact agaaatagaa gctttagtta aagcttctgc gccctgatca cttaaagcaa
     1621 atttgttttt ataaaaactt ttcattgtca agcctccaat catttgcatt ctcataatta
     1681 gtgaccaatt gtttatacac ttgatgttta gccattaatt cattatgatt tccacgttct
     1741 actattttcc cttctgacaa gacaagaatt tcatctacag atttaatgct ggataatcga
     1801 tgagctatca taataactgt tttttctttt atcagatgtt gaaaagcctt ttgtagttca
     1861 tactcattat ctggatcaat agatgcactg gcttcatcca taataacaat ctttgcatct
     1921 tttaaaattg ctctggcaat agctatacgt tgtttttctc ctcctgacag ataaactcct
     1981 ttactaccaa ttaaggtatg ctctctttcg ggaaattttt ctaaaatcga ttcgcaaccg
     2041 gctaatctta gcgcgtcaaa tactttactt gctggcgctt gaggatttgc tatagaaacg
     2101 ttatcgtaaa tactctgatt gaatagtttc gtatcttgaa agacaaaagc aattgtgtta
     2161 ataattgtgt cttttgaata gtgatttaaa ggcttaccac caattttaat ctgtccctta
     2221 tccacattgt agaaacctga aattagttta gctatcgtac tctttccgct tcctgatttt
     2281 cctactaatg cataaattct tttttgttct aaaactaagt tgaaattttc taaaacacac
     2341 tcttctttgt atgaaaagct gacattttca aatgcaatgt cacaattttt aatctgtgtc
     2401 tcgttcccat attttagttg gtcttgcttc atttctaaat aaagggagtc tagtgtttcg
     2461 actgcatatc ttgcttcaaa caagtgcata cttacataca ttattctcat gaaagaaaca
     2521 aaaataattc cacttaaaaa aaacaacatc aacaagtcaa cagctaatat cttgggagtg
     2581 gtaagagaag ttataaaaaa ggttatagga actatcaaaa tgcaaactat gccaaaaaat
     2641 agccattgaa aaagaacaaa aggtgttttc ccactcaaag aatacttata tgcaaactta
     2701 gcataatcgt taattgcttt attcaaggct ttaaatgatg ttactttagc accaaatatc
     2761 tttataactt gaattccgcg gatatattct accgtttcag agcttaacac atctaacgat
     2821 tcttgatatt tttgaataaa atttgtattc tctgtcattt tctttaacaa gaatacacca
     2881 attactaaca tggcgattag aaccaatcca attttaaagt taatgaaaaa gcctaaggca
     2941 actgataaaa caggtgttac tatggcttga gcactatctg gaatcaagtg agcaattgcc
     3001 atatgtgttt tagccgcgtt gtcatcaatt gtctttcgaa tatatcccga agaatttaaa
     3061 tcaaaaaaac gaaaacttgc ttctgttaaa ccgctaattc ccttttttct caaattagtc
     3121 tctaagcgaa acccaaggat atgagaaaat aagccagaaa aaatataaaa taaagctcct
     3181 aaagttaacc aagcaacagt ttgaaccgca taactcttag ctaaatattc atctcctaca
     3241 ataattagag actgaagaaa tttgtaaata ctgtaatatc catagactgt taatacgaca
     3301 gaaaaatcag aaaataaagt ggctaaccat ccgtagtatt tcattttagg gacataacta
     3361 aataattttt tgtatacttt cattttttcc tcccccgcat tcaatttctt ctcttattcg
     3421 tttacaactg ttaatgcttt ccgtaatagc gtaaattaga gatgtacaaa cagataaaag
     3481 gtaaactacg ttctattttt tgtatttttt taagactttt cttccaatca atacaccaat
     3541 catagataaa cttgctacaa ctaacactga aatgattccc catataccta ccgcatagtg
     3601 gatcattgca tccattgttg cagcagattg tcctttatct atccaatctt gacgaaaacg
     3661 atctgtaaag aaccatatag gaatccactg tccggcagca ctgcctaatt gcattagagc
     3721 ccaagacgca acttgaggaa ttgctcgatc atatccaaat acagatagaa taatatcagc
     3781 taagatcgcc atactaatcg aactgataaa ccaaggtaag tacgccccgc ccattaacgt
     3841 aaaaaataac aataaaacaa ttgtgtaaat agtaaaaatt ccttttctag ggtagttata
     3901 gctaatataa acaaatatga ttccagataa taatccaaaa atcccggggg atatgctgtg
     3961 accaaacgcc ccaccaatac tactaaccat tattgaaaaa aagtaaatta ctagggaaat
     4021 aactgtagct aaaccaataa aaatataatc ttttgttttc atatatattc tcctcaattc
     4081 tatttcgtta tgtcattaaa aatctgttct atcaaagttg aatcttctac tctaagtttt
     4141 atatcttttt ttattccacc attttttaaa taaataattc tattgcatgt tttataaaga
     4201 aattccggat catgagtagc tataactact gcaccctttt cttttaagtc tttaataagg
     4261 tcagcaatcc tttccatatt aatataatcc aacccacttg taggttcatc aaatagaaat
     4321 aaattagttt ttgacaatga agctatagaa accaataatc tttgtttttc tccaccggat
     4381 aaatcaaacg gtaattgatc tttcatttta taaagttcca ttttttttaa aattttttct
     4441 tgctgatctt tactaactat ttcattcccc agggctaact cgctcctcac tgattctgtg
     4501 aaaaattggt aatccatttc ttgcattact aaaaacggcg atgacaaatt ttttttattt
     4561 tcgattatct ttcctttggt agggcgaata atcttcatga gtattcttag taaggttgtt
     4621 ttccccacac cgtttttccc taccaaacca ataacatctc caactttgac atcaaaattt
     4681 atgtctttta gaatattttc ataagtaatt tcttcaactt ttaatacatc tgcacttacc
     4741 ttttcgatat agggaacttc aatcgaacta ttaaaaatgt caaatttcct taatgcgtta
     4801 ttgttttcag tttttaacaa ctgaatagtt gtagttttta cagttctatt tttaacaaat
     4861 agaacttgat caattaattc tttcaaatag taaaaacgat gttcagctat aacaatagta
     4921 aatcctttat tttttaaatc acaaagtaag tcagcaagtt tcttagtcgc tttgaagtcc
     4981 aaatttgaag acggttcatc caatataatt aatttagagt ttagcgataa actacatcca
     5041 atagaaattt tctgccgttc accacttgat agcgaaaaaa tgtttttatc taatatattt
     5101 gaaagatcta ataattcagc taattcattg attcttttat ccatatgctc ttttgtaaat
     5161 ccaaagtttt ccatcgaaaa tactaactca ctcgttgtat ttgttgtaaa gaattgtcct
     5221 ctaggatctt gacttacgta gccaatattt tttgatatac tttcttgtgt taaggattgt
     5281 agcgagttat tttcaaataa aatttcccca accaattttc cgtcataaag tcttggaata
     5341 agtccattca ttaactttaa tagtgtggat ttcccactcc cagaagaacc agtaatcaat
     5401 gtaagactgc ctttttttat tgaaaaatta aggtcaacaa gcgcttgttc cttaacatcc
     5461 ttatatgaaa aagaaacatt gttaaattca aacaattctt tcacctcaaa tcaaaatact
     5521 taagaaaaaa atagtaacgg aaaacaggat gatatagtct aataagctca tgtttgtttt
     5581 atagtaagaa cttcttttca caggagattc tatccccttt gttattgcat tagcggtcat
     5641 ttcttcagca atcatagttg cacgtataag aacgggaact aacagaaatt ccaaatattg
     5701 cagaggctga aaaaaagaca agcgaatgtt tctcattttt aaagattctt taatcattct
     5761 tatttcagaa cgataagtcg gaaagaatct gatcactact gtaatactta acattatttt
     5821 tttcggtaaa tgtatttttt ctaaagagga taataattca ttgctagtat agacactaac
     5881 caacaaccat gagatcatta cgaccggcaa cattcgaaat acaatatata aggatgtcgc
     5941 aaaaaatata gtaaaaaact ctatcttgat agcatcaata attttcatta ctaaaaacag
     6001 aactgaataa atcaagatga aataaataca tgtctttatt ttacgaaaca tcaatactaa
     6061 aactgtagca ataatgaaac accccacttg aaatatattt ccctttccta aaaaaataat
     6121 gaccggaaaa aaaatattta aagtaatcaa ggttaacgga ttgatttttg acactctttc
     6181 acctccaaaa aataagatcg cactgttatc ttaagctgat aaaaatgcac cgttattcag
     6241 ataacagtgc gatcttatca aaaagcataa ctgagaacaa ttctcaatgc aagctttttt
     6301 taattaactc agtaattcaa tccacccagg aacagaaaat tgatacaatc ttctggttaa
     6361 tagcatagtc ttttcttttg aatagctatg cttcaatata tcatagaatg tagcgtagaa
     6421 tgagtaaatg tagaagtgga tttcttcttc attaaagact aaattttgat tgatcattcc
     6481 gatagtcttt aacttatcta gacaacagac tgtctttttc gtcacaaatg atgtaatttt
     6541 ttcaacaata tcttgatatt tggttccagc tgattggaat aaaatcaaat cgaatatacg
     6601 tttattctca aaaatataat taataaatgg atctaattca ttgaagttct ctctccaact
     6661 tttctctata tctatatttt ctaagtttaa gaggtatttg gtaaactctt cattatacat
     6721 attaataagc gcattaatat gcggctgaat aatttcttca aataacgctt ccttagaagt
     6781 aaaatgttta taaaaagcac ccgtagttac gcctgcggtc aggcacagtt gccttatctt
     6841 agtttgctga aatccttctt tttcaaaatg gcttagtcca ctttctatta acttttggtg
     6901 agttataata taatctctgg tttctatggg tacttcctcc taataagatg aattaacttt
     6961 attatcttac acaggacttg aagtttcaat cgttaacgtt tattactgaa ctcattcctt
     7021 agagctaaac taatgagaaa gagcagaata actgatacaa aaagcaatcc tacaagaatt
     7081 aatagactca tctaaatcat tcaccttttc taaaataatc cgagaaagat ttaaaaaaat
     7141 tatatccagc tattaggata agtcctatca taatcactcc aatagccatt ttcttatcct
     7201 ccctaaaaat aaagtctttt acttaaaacc ttttttgtaa tcgtcaataa attttttccc
     7261 tttatcagtt aattgaaatc ctatatctaa atagtcctca tatctaagtt tagccagcat
     7321 ctcacttaca gatgctggac taacacttag aacttttgct aaatcattat tttttaattt
     7381 cttttttctt tttgataatt cctgaattgc tagtaaataa attagtatag tagtcgtttt
     7441 aatttgtacc atacctaccc tccaaattat ttcgaaaggc cttcagcaat tttatccagg
     7501 ttccatttca tcatcgcata gtaactatca ccatcttgtc ctttttctgc aattgaatca
     7561 gtaaaaatcg ttgaatagat aggaatattg gtatcttttg atactgtttt cattggtcta
     7621 tcatccacac tgctttctac gaataaggag ggaacttttg ttgtgcgtaa tttttcaact
     7681 aagtgtttta tttgatctgg tgttccttct tcttcagtgt tgatttccca aatgtaagca
     7741 gaaggcacat tatacgcttt cgagaaatat ttaaagcatc cttcacttgt aacaatcatt
     7801 tttttatcat tcggaattga agcaaattta gatttagctt ctttgtctag agaatccaat
     7861 ttttcaatat acttatctag attttcttta tagaattttt tattatcagg atctttttcc
     7921 gctaactgtt tttcaatatt tttagcgtaa ataataccgt tttctaaatt taaccaagca
     7981 tgaggatctt ccttcccttt ctcactctga ccctctaagt aaataacatc tatgccatca
     8041 cttgctgcaa aatagtcttt gttttcctct ttgttcgcat ttttaactaa ttttgtaaac
     8101 caagcatttc ctccagtttc caagttaaca ccgttataaa aaatcaaatc tgcttttgaa
     8161 gttttttgaa catcttcagg caaaggttca tattcgtggg gatctttccc aataggtacg
     8221 atactgtgta aatcaatttt atcttttgct atattttcgg taatatctgc taaaatcgaa
     8281 ttagtaacta ctacttttaa tttttcgttc gaagccactt tatctttgtc tttactattt
     8341 attgaacatg ccccgaatat aaaaagagca atcaaaatcc ccagacttcc cagtactgct
     8401 ttccattttc tcatcataac gatctctcct tctttcttaa aaatttttgt tttggtgaga
     8461 ttagaaaact aattacaaat aagaatgccg ctgttaaaac aatacttgat cctgctgcta
     8521 tgttaaaact ataaccaata aacaatccaa gcaatgatga acttgctccg actgttgcag
     8581 aaataaatat catagttttc aagctatttg catataaata tgctgtagct gctggtgtaa
     8641 ttaacattgc aacaattaat atagttccca cactttgcat agctgttact gctactaaag
     8701 ttaatagaac catcaataga taatgataaa aactgacatt catccctatt gcttttgcca
     8761 ttaatggatc aaaagaagtg attaataatt ctttaaaaaa tagaactact ataaccaaca
     8821 ctaaaaccga aacgccaatt gtaatccact tatcaatatc ttgtacagct aaaatatttc
     8881 caaataaaat atgaaataaa tccgttgagc tatttgcaac tcctataagg ataactccta
     8941 gtgctaaaaa agaactgaaa gtaataccaa ttgctgtatc tccttttatt acactattgc
     9001 tttttataaa agtaataatg atagaagcta atagaccaaa tacaatcgca ccaataaaaa
     9061 aattaatacc taaaatataa gaaattgcta ctcctggcaa aacagcatga gaaatagcat
     9121 cacccataag agacattcct cttaaaataa taaaacaacc tactgttcca gaaacaatcc
     9181 ctataactac agatgtaatt agagcattct gtaaaaagtg aaagtcatac agaccattaa
     9241 taaaattttg aagcatatta ttccccctct ccaataaata tagtatctcc ataggctttt
     9301 tttaaatttt cttcattaaa tacagattct acacttccgt gagcaattag atttcgattc
     9361 aaaagaataa tattatcaaa atacttcttt accttgctta aatcatgatg cacaattagc
     9421 actgtttttc cctgatgttt caattttttt aacgtatcca taattattcg ttcactaact
     9481 aagtctatcc ctacaaatgg ttcatctaaa aatatgaaat ccgcattttg agcaagacat
     9541 cttgctaaaa gtacacgttg aaattgtcct ccagataatt ctcctatttg atgattagaa
     9601 tattttccca tatcgacctt ttccaaagcc tttgaaactc tttgccattc tgcattttta
     9661 attctttgaa aaactttcat tccggcatat gttcccatag atacacattc ttttactttt
     9721 attggaaaag taaaatcaat atcagctttt tgctctacat atgcaatctt caccagtttt
     9781 ttattgatat cttcattatt aatggagacc tctccctgat gagggataat atttaacatt
     9841 gctttaagca atgtggattt tccagcacca tttggaccaa taatacctgt taaagcacct
     9901 tcttgtattt ttaaagaaat ctcgttcaat gctaaaaaat cgttataaca aacattaagc
     9961 tttttcactt ctaacataac tttctccttc taaatgataa tgattatcac gatcatatgg
    10021 tatacaaaaa ttatatttta gtcaacccta aattttatag tttagatttt tttctgttgc
    10081 tgaagattag aatgacaaaa ctatttgaaa ctacgcgtat agtaagtagc tttcaaaata
    10141 tataaaaaag gagcgttctc aaatgagaac atgctcctaa aactacttta ttggcgtatg
    10201 gaaaaattat acttgaagtg gatagcgggt tgaataagtt gataagcatc aaaacattta
    10261 taaaaattat tcaagactat gtctgttcca tatacaaata tgaaaatcag taaactccat
    10321 ttaacttttt tcgtagtgtg tctttttata catgtttatc gttattaata gtgaatagga
    10381 aaaaaactat ttttgatcat tgacaattaa atacaatttt tgaataacga tccctgctat
    10441 tcgagctaga aactcaagta cgccatgacc tgattagcga caaatactta gagatttctt
    10501 tttgttctgg gaaaacaaaa tgccatgaca atagcagcaa ataatgatac ttccgtaagg
    10561 ctcgccgtaa actggggaga ggtgaaattc ctatgtgctt acccaaagca attcaaaaat
    10621 ttcataacta ctattttagg agatgatttt atagaatcca gagtctacct gaataaaaaa
    10681 caaacctgtg catttctagg aatcgataat aatactctag ataagtggat tcgtatatat
    10741 aatttcccag taataagagt tggtggtgta gttcgctttg acgtagaaga tattaaagac
    10801 tttatgaata catttaaaat aacttgaaat tttcttctcg ccgcatatac taaaattagt
    10861 gaaatatact gccttacttt ttttaaggag tgaggatttt gcctacaaag ctttcaaatg
    10921 ggaaatataa aactaatctg agatatccaa aaaggtttaa agaaataaca ggaattgcat
    10981 cagaaaagta tcaaaaaaca tttccaaata ggcaattggc gattaaagcc gaaaatgata
    11041 tgaagaagaa aatagaaaaa gttttgagag aggagaatgc aaactcatta gaattaaaag
    11101 gaaaaataac gtttaaaaaa ttttacgaat ctaaatggct tcctagatat gaattaggtc
    11161 aaacaattag atcaaacagg cctcctagtg atataaccat tagtaataca aaagatatat
    11221 ttcggttgca tattttacct atgtttggtg aatacgcaat ga
//
LOCUS       DACDIG010000082        11199 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.082, whole genome shotgun sequence.
ACCESSION   DACDIG010000082 DACDIG010000000
VERSION     DACDIG010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.082"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(20..955)
                     /gene="murB"
                     /locus_tag="IV247_002369"
     CDS             complement(20..955)
                     /gene="murB"
                     /locus_tag="IV247_002369"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAP8778419.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1028..1948)
                     /locus_tag="IV247_002370"
     CDS             complement(1028..1948)
                     /locus_tag="IV247_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8778420.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2061..2813
                     /gene="xth"
                     /locus_tag="IV247_002371"
     CDS             2061..2813
                     /gene="xth"
                     /locus_tag="IV247_002371"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAP8778421.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            3677..4210
                     /locus_tag="IV247_002372"
     CDS             3677..4210
                     /locus_tag="IV247_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAP8778422.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(4700..5224)
                     /locus_tag="IV247_002373"
     CDS             complement(4700..5224)
                     /locus_tag="IV247_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8778423.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(5217..5690)
                     /gene="tsaE"
                     /locus_tag="IV247_002374"
     CDS             complement(5217..5690)
                     /gene="tsaE"
                     /locus_tag="IV247_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAP8778424.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(5813..6793)
                     /gene="pta"
                     /locus_tag="IV247_002375"
     CDS             complement(5813..6793)
                     /gene="pta"
                     /locus_tag="IV247_002375"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAP8778425.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(6946..7626)
                     /locus_tag="IV247_002376"
     CDS             complement(6946..7626)
                     /locus_tag="IV247_002376"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP8778426.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            7752..8621
                     /locus_tag="IV247_002377"
     CDS             7752..8621
                     /locus_tag="IV247_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8778427.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            8647..9162
                     /locus_tag="IV247_002378"
     CDS             8647..9162
                     /locus_tag="IV247_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778428.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(9209..9772)
                     /locus_tag="IV247_002379"
     CDS             complement(9209..9772)
                     /locus_tag="IV247_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8778429.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(9827..10006)
                     /locus_tag="IV247_002380"
     CDS             complement(9827..10006)
                     /locus_tag="IV247_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778430.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(10083..10592)
                     /locus_tag="IV247_002381"
     CDS             complement(10083..10592)
                     /locus_tag="IV247_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778431.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(10688..11068)
                     /locus_tag="IV247_002382"
     CDS             complement(10688..11068)
                     /locus_tag="IV247_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP8778432.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
BASE COUNT         3214 a         2288 c         1992 g         3705 t
ORIGIN      
        1 ttttaagttc cattacttat taattggcta aacttctgct tgtttctgat attgcttgtt
       61 tttctccaat gatccgcact tctgtttcta aagacacatc aaatttttct ttgattactt
      121 cttggatgtg cgcaatcaat tcaatataat ctgtcgctgt cgcatgatcg atattgacga
      181 tgaatccagc atgtttttca gagacttgtg caccgcccca tttcaggcct tgcaatcctg
      241 cttgttggat cagctgacct gtatagtgtc ctactgggcg cttaaagaca cttccgcatg
      301 aagggtattc taatggctgt tttgattgac gcaattcggt caactcatcc attcttgctt
      361 tgattgcttc ataatcccct gtttgcaaac tgaatcgagc ttccaatacg atagcacgta
      421 attcttggac tttactgtga cgataagaaa ataccatttc gtcttttgtc aaagttttca
      481 atgttccatc ttcaagcaaa agatccactt cagcaaaaac atctttgatc tctccgccat
      541 acgcacctgc attcatataa accgctccac cgacacttcc cgggatgccg caagcaaatt
      601 caaatccagt caatgattct gccaaagctg cataagtcgt atcaatcagt gaggcacctg
      661 cttccgcaat gaccatcgtt cctttgacat gaatctgttt catttcagtc agcatgatga
      721 ctacaccgcg aatcccgcca tcttgaacga tcagattgct tgcatttcct aataccatcc
      781 aaggtatatc atggattcgg caataatcga tgatttgctt tacttcttct tgttttttcg
      841 ggaatgcaag gacatcagct ggtccacctg ttttcgtaaa cgtatagttc atcaatggtt
      901 catcaaacaa tatagtaatc gtggctaagc tttcaactat ttcttcttta ttcactgagg
      961 attttccttt cttttctcaa acgtcctcat tattctatca cgcttaatac aatcttgcca
     1021 gtcttgttca aagttgatga cggtagtgat ctttaagcac ttcctcacat aattcatgac
     1081 ttcttacaac ttctttttgt ttaagcaatt ctgtttccga tttacctaaa cggatctgag
     1141 aaaagaaggc ctctaccatt ggctcaaacc cgcgtttgac tagcgtgttt gtccagtcgc
     1201 caaattcttt cacttttttt ccatcagcat cttgaatgac aagctgggtc agatcgttta
     1261 cttcataagt accattttta cttgttactt gatatttttc tatattagcg ccacttgcta
     1321 aatccatcgt taaaatagcc gtttgatttt gtgtttctaa ttgtaaaatg gcatattcca
     1381 aaaattcctt tgtttctctt atctggtatt ttgttctcaa aatgggttca tctaataaat
     1441 acacagctgt atcaataaga tgtaaaaaca aatcataaag gacgaactct gtcgtttctt
     1501 ttgccgctat tcgatttttt tgcaggtgga tcaaccgctt atctgggata cgtttcaatt
     1561 cttctaccat aggagcaaag cgccggttga aaccgatcat caaaattatt tggttcttat
     1621 cagctaattc ttgcaattca cgaacttcat ttagatggac actcagtggt ttgtcaatat
     1681 aaacatggat accattttct agacattttt tggctaattc ataatgtact tttgttgctg
     1741 cgtggatcat acatgcttcg attttttctt ctaaaagctc atcgatcgtt tcatatagat
     1801 gttggaaccc gtactctttt tgaattcttt cttttgtttc tttatttctt gttgcaaaat
     1861 aaaaatcaag tgttccttgt tttttcgcat aagttggtaa atatgctttt tctgcgatat
     1921 tccctacacc gattacacca atcttcatct actttcctcc tggatcattt agttactttc
     1981 atcttacacc attttttact aaaatgaggt acaatagtat cgcaaaggta aggaaattaa
     2041 ttcgaaggag tgtgctttta ttgaaattta tctcttggaa cgtaaacggg cttcgtgcca
     2101 tcgtaaataa aaatttttta gaagtttttc atgagttaga cgccgatttt ttctgtctgc
     2161 aggaaacgaa actccaagca ggacaaattg atttagatct tccaggttat tatcaatatt
     2221 ggaattacgc ggaacgaaaa ggctattccg gcacagcgat ttttgctaaa aaaccagcat
     2281 tgaatgccac ttacggaatg ggtatcgata tccatgatac agaaggccgt ctgattactt
     2341 tggaatattc cgatttcttt cttgtaacct gttacacgcc aaattctcaa agcgaattaa
     2401 aacgtttaga ttaccgatta gaatgggaag aagcctttta taattatttg gaaaatctaa
     2461 aaaaacaaaa accagtcatc gtgtgtgggg atctaaatgt cgctcatcaa aagattgatt
     2521 tgaaaaactg gaaaacgaat caaaaaaatg ctggattcac tccagaagaa agagcggctc
     2581 tttctcgact gttggataac gggtttatcg atacattccg ttatttttat ccaacccagg
     2641 aaggtgtcta ctcttggtgg aactaccgat tcaattcaag gaaaaacaat gctggttggc
     2701 ggatcgatta ctttttgacg agcaaagatt tagagccagg actagctgat gcaaagatcc
     2761 atacggacat tatgggcagt gatcattgtc cagtagaatt agatttgaaa taaagaggtt
     2821 gtgaaaaagt cgtttagttt cgagtattaa gaaaaatttt gtgaaaatgg ctttccacat
     2881 tttgcataaa tttggcataa taccgagaaa ctggcttttg aacactgttt atcgagagat
     2941 tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca aacaaaaata gttcctcata
     3001 tttttcgttt ggttgggctt attaccgcag atacaagctt ttgaacaccg tttattaaga
     3061 ggttgtgaaa aagctgcact gcatcaagta ataagaacag ccaaacaaaa atagttcctc
     3121 atatttttcg tttggttggg cttattaccg cagatgcaag cttttgaaca ccgtttatta
     3181 agaggttgtg aaaaagctgc actgcatcaa gtaataagaa cagccaaaca aaaatagttc
     3241 ctcatatttt tcgtttggtt ggacttatta ccgcagatgc aagcttttga acaccgttta
     3301 ttgagaagtt gtgaaatcag cgtcctgacc tgagtattag agaaacaaat ggcaaaatag
     3361 ttcctcatat tttgctattt tttgagctaa taccgagggt cagctgattg aacaccgttt
     3421 agtggagaag ttgtgaaaaa gtcgcctact ttcgagtcgt aagaaatttt tttgaaaatg
     3481 gttcctcaca actttgcata aatttgactt aataccgaga agtcatgatc cccatctaac
     3541 tccaaatcac aaggaatgag ttcaaaaact agcttttctc aacaaatatt acataaaaaa
     3601 ttctatgttt tttgttaaat ttatttgctt ttttcaaaag tatgttacag taatttccca
     3661 aggaggtttt acagaaatga attttgttgc catggatttt gaaactgcca atcatcagcc
     3721 ttatagtgct tgttctcttg cgctagtcat ggtaaaaaat agccagatcg ttgatgaatt
     3781 ctatacattg atacagcctg agactccttt tttctggcga aatgtccaaa ttcacggaat
     3841 ccatcaagaa gacgtacgca atgcgccgaa attcccagaa gtctggcaaa ctatccaacc
     3901 atattttcaa aaaaatcgtt tagtcgttgc acataatgct gcttttgata caaaggtctt
     3961 agcgggatgc ttagattatt atcatcttga acagcctaat tatctttctt tatgtacagt
     4021 gaaaagtagc cgtcgcctgt ttcctgaatt tccaaatcat cgattgaaca ctgtgtgcga
     4081 aaatttgaac atccaattag atcatcacca tgatgcattg gaagatagtc gtgcatgtgc
     4141 ggaaatcctg ctttatcaag aaaaaaaatt cgggacagat ccattgaaaa aacttgtgac
     4201 tataaagtaa aagaatactt atgaaacaca caaagaggtc gtgagcctgc cgtttcactc
     4261 cgagtcacaa ggagcaattt caaaaaatag cttctcatgt ttattaaata ttgtgactgg
     4321 tgaccgagga gctggcagga gaacaccgtt tattggagag gttgtgaaaa agctgttctg
     4381 catcaagtaa taagaacagc caaacaaaaa tagcttctca tatttttcat ttggttggac
     4441 ttattaccgc agatgcaagc ttttggacac cgtttaccaa gaggttgtga aatcagcgtt
     4501 ttgacctgag tattagagaa acaaacggca aaatagttcc tcatattttg ccgttttttg
     4561 agctaatacc gaaggtcagc tgattgaaca ccgtttattc aaaaaaaatg gactgcgaca
     4621 cgacttttgt cacagtccct tcgtgtgttt ttctgccttc tcaaaaggat gacagtttct
     4681 cactcttttc ctatttcttc tattcaatca aataactcat tttatataca tctaaaaatt
     4741 cacctagatc tgttcttgcc cctctaggca tgaccgcttc gatttggaag cccatttttc
     4801 ggtaaaggtg tacggctctt tcattacgaa cttgaacgtc aagctccaat cgaaaaagta
     4861 cacccatttc cttcgcccaa ctgatgattt cttctagcat aagagtgcct agtcccattc
     4921 cccaatattc ctgcagaata ctgataccga cttctcccac gtgagacaaa cgaaattgac
     4981 tgtctgcctt gacagatgct gttccaataa tcgtcccttc atagattgca agcagcaata
     5041 aattattatt gctttcgtat atataatcca actcttcttc taaggtttct ggtggtaaca
     5101 ggagttctgc ttcatccaaa actaaaaatt ctgtctcctt attaacttgt cccatcatca
     5161 aaagaatgtc ttttgcatct cctggcactg cttcccgcaa gatgatccca acttcttcac
     5221 tcatgatttt cagcccattt ttctagaatt cgctgtttga aggtttcact ttgctccccg
     5281 tatgagatta gtttcacata ccgcttttct aaatctgtat cacttttttc taaaatcagt
     5341 tccaaataat cctccggcag tgcttcttct aatagatttc cccattcaac aacggaaagc
     5401 ccatcacctt caaaatattc atctaaccct aaatctgctc cgctagctgc tacccgataa
     5461 atatccatat gatacaacgg aatccgacct tgactatatt ctctaataat cgtgtatgtc
     5521 ggacttttga tcatttgcgt tatccctaat ccttgggcaa ttcctttcgt caacgttgtt
     5581 ttacccgcac ctagatcacc agtcaatatc agattgtcac ctggcattgc ggaatgacca
     5641 attgcctctg cgaattgttc tgtcattgtt aatcctgata gttcgatcat ttttcatctt
     5701 ctcccgctct tctttctttt tgccagtata tcgcgtaatg aagttttttt aaagaaaaaa
     5761 tgaactagtc aagaacagca gctgctgttt ttggctagtt cgcgtgtatt tgttaatcaa
     5821 tcaatgtttg tgctgctgtg ataatcgata atttatatac atcttcttca tttgctccac
     5881 gagacaaatc agaaaccggt ttgttcaagc cttgtagaat tggtccgatt gcttcaaagt
     5941 taccgaaacg ctgagcaatt ttatagccga tatttcctga ctgcaattct ggaaatacaa
     6001 agactgttgc ttgaccagct acttctgaat ctggcgcttt cagttgtgca actgaaggaa
     6061 cataagaagc atcaaattgc aattcgccgt cgatcatgta ttgtggcgct agttttttag
     6121 cgattttagt tgcttcaact actttgtcta cttctggcga tttcgctgag ccttttgtcg
     6181 agaagctcat cattgctact tttggttcga tatcgaacaa ttctgctgtt tttgcagaat
     6241 ctacggcgat ttcagccaat tcttgcgcag ttggattcac gttgatcgca caatccgaaa
     6301 agacatattt ttcttgatct cttccacgta ccatgatcat cgctccgctt gtccgactga
     6361 tgccaggctt tgttttgatg atctgaagtg ctgggcgaac agtgtcgcca gtcgaatgga
     6421 tcgctccaga gaccattccg tctgcaatcc ccatatacgt aagcatcgta ccaaagtaat
     6481 tcacatcttt taaaatcttt tctgcttgtt ctttggatgc ctttccattt ctacgttcaa
     6541 caaaagtatc caccatttca tcccacttat cgtaattatc agggctgatg atcgtaaagc
     6601 tagatgtttt gattcctctg gcatgcgctg catttcttat ttcttcttcg ttccctacta
     6661 aaatgggttc cacaagttct tctgctttca accgggcagc tgcgcctaaa atacgagcat
     6721 cagtagcttc cgggaaaacg attttgatcc cacggcgaac aattttaaat cttaaactat
     6781 caaacaattc cacaagatga cctccagacg tttttatatg tattatcata cactattcca
     6841 gaaaaaaaga acagtttatt ttatctgtat cagtcaaaaa tgctggaaaa attaagtttt
     6901 tcacatacaa agcgaactgc actataacag gaaagcgctg ttttattaca ctgtttcagg
     6961 cagttgccaa tcaatcggtt cttctcctaa cgcaaggagc gcatcattcg ttttcgagaa
     7021 gggtttggaa ccaaaaaagc cacgacttgc agataacgga ctgggatgtg gagatgttat
     7081 gatgatatgg cgactcttat cgatcatttt gattttttct tgtgccggtt ttccccacaa
     7141 gataaacacg attggttttt cccgttcatt taatttttca atgatcgtgt ctgttaatct
     7201 ttcccaacct ttcccgcgat gagaatacgc ttgcccttcc cgaacggtaa gaacggtgtt
     7261 gagtaaaagg actccctgtt tagcccatga cactagattt ccatgttgaa caggtgcgat
     7321 tccaagatca tcataaagct ctttatagat atttcttaac gatggcggga tctttacgcc
     7381 aggttgcaca gaaaaagata agccatgagc ttgattttct ccatgataag gatcttgtcc
     7441 taaaatcacc actttaactt cttcataagg agtcaattcc aatgcttcat agatatgata
     7501 catatctgga tggatctttt gggacgcata ttcttttttt aaaaattctc ttagatgcag
     7561 ataataatct tttgaaaatt ctgaggacaa aacttcttgc caactgttat gaatgatttc
     7621 tttcaaaaac tccacctcct aataaaaaga ataactcaca ttttcttcaa tcacaaggga
     7681 caatcttcac cctcagcact tgattaatat ggtaaactag gaacaagaag cagaaaggaa
     7741 cgataatcat tatgatcaaa ttaattgcat cagatatgga cggcacatta ttagatgccc
     7801 atatgagtgt ttctcctgaa aatgccgagg cgatacgttt tgcaaatgat gccggtgttg
     7861 agttcatggt cgctactggt cgaaactatc aagaagctcg cgcagcttta gatgaggtag
     7921 gaatcgattg tgcgatgatc actttgaacg gggcacaggt atttgataaa gaaggaaatt
     7981 cattgtttac cgtgtcgatt ccaaataatc aagcaatctc tgttttagat attctagatg
     8041 ctaacggtat ttactatgaa gtagcaacca acgatggtct ttattctgaa aatcaagcga
     8101 aacgaattga aagttttgct tccatggtgg ctacccattt acctcacctt acgtataaaa
     8161 tggcgattgc catggcctca gctaagctgg agttattgca catcacatac gtggacagta
     8221 ttcgtgctat tttggaagaa aagaatctgg aagttttaaa aattatttgt tttcatacag
     8281 aagggccagc tgtattaggt ccagttggaa agcagatttc tcttttaggc gatctagccg
     8341 ttacttcctc tggtcaaaac aatatcgaag tgaaccataa aaacgctcaa aaaggaattg
     8401 ctgttgccca tgtcgctcac gagcgtggga tttctttaga agaagtcatg acgattggcg
     8461 ataacttcaa tgatgtcagc atgctgcaag tagcaggagt cagtttcgca atgggaaatg
     8521 cagaaatcga agtaaaagat tatgccaaat atacaacgga cacaaacctt gaatctggcg
     8581 taggaaaagc catattgcgc gcgattgcag aagacttgta gttttatcta gaaaagaggg
     8641 atatagatgt cagaattttt cattcaggaa aagcagcttg gacgtgcaac tcggacaatt
     8701 gtcaaaaacg aaaaaggaca ttccctgttt ttgatggttg gtcgctgggg aacaaagggc
     8761 gatgcattgt ctctttatgc catgaatggc gatctcgttg ctcacatcaa gcagataagc
     8821 ctgacattcg gtacgcgttt tgagctgtat aaagagttcg aaaaggtggg aaccatgcag
     8881 aaaatattca attggcccgg tgacttttat tatattcgta gacttcactg gacagtccaa
     8941 ggtgatatct ataatcatca atatcaaatc catcatttca atcagcaaat catgaaaatg
     9001 gataaagcaa cacttttttc aggagattac tatgtattgg atgtctcaaa tgatgaagat
     9061 gctccagttt gtatttgtat tgctgcggtt ttagattact ggctctaccg taaagacaaa
     9121 agtcgcgata agcctgtttc ttaccgactt aacttaagtt aaaaagatgt acctgaaatg
     9181 cggaaatacg catttcaggt acatcttttt agatattctt ttgcatatga tcataaagat
     9241 gaccactgat catccgctga ccgacaactt tgaagtcatg gcgttcgtat aattttttag
     9301 cctttggatt tttttcatcc acacttagac cgatgacgga gcgattagct ttcttcgcca
     9361 atttaggtaa tgcttctaat aatcgcgaac cgatgccttg tcctctaaaa tcttctgata
     9421 cactgataga gtccaaatac cattcgttgg gaaacgtttc tttatcaatg aacaaacgtg
     9481 tagacgcatc tagctgatat ttctctaaga taggtaccag tggctgatcg atgattgatt
     9541 cttcttcatc tgtataaccg taagcaactc ctgcaacttt accgtctatt tcttctacga
     9601 tacctcgtga ataactgtaa cgataatttg gatcagtgat tgcttcttct aaaatctcta
     9661 aagtcttttg ttcccctgtc tgcttcaaaa atggcagttc catatctttt agaatcacta
     9721 agatcaatgg ggcgatttct ggagcatctt cttttctcgc aaaacgaatc atagtttcct
     9781 ctccttttct ccattctaac atgcagaaaa agaaatcgca aaatcgttat tttaaaaaat
     9841 cttcaatctt ttcagtaatg aaataaccat gagtaacgat tcttgccgct tcagttcggc
     9901 aataagggca gacgactggc atttctttat cattatgttc gactacatta ttgacttctt
     9961 cgtatgcaaa tttttttccg caattagggc atttcatttt ttccatgtta tctcaacttc
    10021 ctttcatctt tactctctta gcttactctt atattgtaaa aaagagaagc aatatccttc
    10081 ttctatgcag atatccatac ctgacggtcc tgttctccat ctattaatcg gactttataa
    10141 taaggacgtt catctttgac tagaagtgtc cactctttca cttttgcttt tttcccgtag
    10201 tagctgactg cttccttcat cgctgcttgc ggttgtttga actcttccgt tttttctata
    10261 tcaaaaagat gttctgcggc cttttccgtg tcatttcgta cggttgtttt cccagtggat
    10321 ggattgactg ctatgacttg tccttcacaa aaaaactggt attcatatgt tgcattcgta
    10381 ggattctcca aatctttttc aactgcaaaa tgaatagcag atatgttatg ttcttttgtt
    10441 tcgtttttta cgattgctgc tgcttttttc aatgagatcc gatattcctg gtcactaata
    10501 tcatccatgt gaaaagcggc attgagcagt cccgtcatac cggtaatccc tattacgatg
    10561 agggcagcaa caccaaacat catttttttc attcttcatt ctcctgtttc ttttttttaa
    10621 agaatatcaa gaaatgaacg aatgaaaaat cgaatgaaac gaaaaaaaag tgacaatttt
    10681 gttatcttta ttcgtgcagt tctaatggca atccgtctgg atcaaagaaa aacgtcattt
    10741 tttttcctgt aaaggtatct tctctaatcg gttctgattc gatccctaag gactgcaatt
    10801 ctgcaattgt ttcttcaata tcgtctactt tgaaagcaag atgacgcaag ccacaagctt
    10861 ctggatagtt tggtcgttgt ggcgcattag caattccaaa aatctccaat tcagcacttc
    10921 ctagtttcag atctaattta tagtcatctc gttctttgcg gtaattttct ctgattactt
    10981 caaatcctaa tttattaaca taaaaatcac gtgatttctg ataatctgaa acaatgatgg
    11041 ctatatgatg gatcgttgta aggttcatat gagcactcct attcttcgtt taagctcgga
    11101 tctatattaa tctccagttc cattgtaaat gtgtcagatt gaaaaaaata gctgtttttc
    11161 ctttttataa gaaaaaaggc aaaaaaaaca gaaagtagg
//
LOCUS       DACDIG010000083        11152 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.083, whole genome shotgun sequence.
ACCESSION   DACDIG010000083 DACDIG010000000
VERSION     DACDIG010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.083"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            11..1381
                     /locus_tag="IV247_002383"
     CDS             11..1381
                     /locus_tag="IV247_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP8778433.1"
                     /translation="MSFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIA
                     LTIIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLS
                     KQIKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISD
                     SIIQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWF
                     LFQKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSI
                     AAKENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVG
                     ESWEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNV
                     DVNAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEIN
                     KKNENK"
     gene            1368..3002
                     /locus_tag="IV247_002384"
     CDS             1368..3002
                     /locus_tag="IV247_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778434.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            3177..4010
                     /gene="msrA"
                     /locus_tag="IV247_002385"
     CDS             3177..4010
                     /gene="msrA"
                     /locus_tag="IV247_002385"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP8778435.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            4090..5079
                     /locus_tag="IV247_002386"
     CDS             4090..5079
                     /locus_tag="IV247_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAP8778436.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            5368..6066
                     /locus_tag="IV247_002387"
     CDS             5368..6066
                     /locus_tag="IV247_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAP8778437.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            6216..7532
                     /locus_tag="IV247_002388"
     CDS             6216..7532
                     /locus_tag="IV247_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8778438.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            7549..8964
                     /locus_tag="IV247_002389"
     CDS             7549..8964
                     /locus_tag="IV247_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8778439.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            9429..9749
                     /locus_tag="IV247_002390"
     CDS             9429..9749
                     /locus_tag="IV247_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8778440.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            9775..10092
                     /locus_tag="IV247_002391"
     CDS             9775..10092
                     /locus_tag="IV247_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP8778441.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            10121..>11152
                     /locus_tag="IV247_002392"
     CDS             10121..>11152
                     /locus_tag="IV247_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8778442.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVL"
BASE COUNT         3709 a         1767 c         2234 g         3442 t
ORIGIN      
        1 tacgattctg attagttttt attatggaga aaagtttttt aaaaagatag atgctgcttt
       61 tttatttagt tgtttatata cttttgcaac ttatcgaaca gttgatatct attatcgatc
      121 cgcaattgca gaagcaatcg ctcttacgat tatcccagct gttttatatt atgcttatgg
      181 attgatattc aatagagaaa aaaaagtcat cgctttatct gtcagtataa gtctacttgt
      241 gtatactcat gtcttaacta cactattgtg tagtgcattg attgcttgta tgattatagt
      301 tcgtttgttt ttaagcaagc aaataaaaaa cgatataaaa tttgtggtcg tgaatttagt
      361 aaaggctgtt tttttgactt gtgcattaac tagctatttt tggtatccta tgttgcagca
      421 gatgaattat caagaaatac acagtccctt caaaacagat ttgcagctag aagcactttc
      481 tatttcagat agtatcattc aagcgttaaa taacgatatt acaacataca ctatggggtt
      541 attaggattg gttgcgttga tttttccttt attcatactg aaagaaatgg agaaaaaaga
      601 aaaaatcatc tatgttggcg tagtttctac atgggtatgc gtaactaatt tatttccatg
      661 gtttttgttt caaaaaacac cgataagctt actccagttt ccttggcgta tcttaggtat
      721 ccaaatcttt ttttcttcaa tgatcataac gcttgttttt atgaaaaaga ctatgaataa
      781 aaaatattca tggttaatca ttggcgctac tgtggtgttt ctactcgtca cgtctatagc
      841 agcaaaagaa aattacgcaa aagttatcca atcaaaacac ggtcatattg ttatcaatga
      901 agaaacactc ccacgttata caacatcaga tatgggagga ttatacgatt acgccccaac
      961 agaagcgata aaagaaaggc ctcacttaga aaaacatgaa gtaaaagtag gagaatcgtg
     1021 ggaaaaaggg aagtataaag ctgcagataa taacattact tacacagtgg caagcaacaa
     1081 aaagcaaaaa gtgacagtac cagtatatgc ttatttaggt acacaggtaa aagtaaacgg
     1141 taaagtcgta aatgactctt ataaaaaaca tggcttagtg aatgtggatg taaatgctgg
     1201 agaaaataag atagaaatta caacaaaata tactccaact gctctcatgg ctttatggat
     1261 cagtattctg acttattgtt tagtggtgta ttcaagtttt aaacgatttg cacctaataa
     1321 gttgaaagat tctcgatatg tgactaaaaa ggagataaac aagaagaatg aaaataaata
     1381 aaaaagtagt aatattgagt ggattaatta tagttagtag tatatattct ttcatctttt
     1441 tcaaaaatac acagagtatt tataccactg gagatctttc ttttcactta tctcgaatca
     1501 aggggttatc gactattttt accagtccga ttaattatga gacattcaat tatactggat
     1561 atggtgtaaa ttatttttat ccctttctga ctttttttcc agctgtaatg ttatattgga
     1621 tgacaaaaaa tttgattgta agctatatta tttacgtttg gttattgaac ttgtgtacaa
     1681 cactcgtagc ttatcattat ggcgagcggt ttttaaaaca aaaaaaagcg gcatttttat
     1741 ttagttgtct ctatattttc tctgcttatc ggacagtaga catctactat cgttccgcaa
     1801 ttgccgaagc tatcgcaata acacttgtta tccctgtatt attttatgct tatcaaatca
     1861 tctctggaaa agaagaaaag tatccgtctg taaagctagc attgagtatg agtttgctag
     1921 tgtacagcca tgtactatca acattaatga gtacagcatt aattatcatt tttattttta
     1981 ttcgtttggt ttctaaaggt ttcaagaatg cagattttat tgctatattt aaaaaattat
     2041 ttagtgcggc aggaatgaca ttggttctga cgagtgcttt ttggtatccg atgtttgaac
     2101 aaatgctcta ccaaaaaatc aacaaaccta gcgtgacaaa tttatatgct catgcatcta
     2161 atgtgttcga tagtctgact gaagctatga acaacgactt aactacttac tcaatgggac
     2221 tagttggtct gttatcttta tgtattcccc tgattttatt taaaaaatta acaaacatag
     2281 aaaaaaagat ttattatggt acgtgtttga cttggctagc tacaactagt ttggtgccat
     2341 ggtatcttct gcaaaatacg ccagccaaat tgttacaatt tccttggcgg atcttaagct
     2401 tgcaaataat attctcttct ctgatattaa cgatgatatt tttcaaaaat agaagataca
     2461 ataagacaag agaactattt tatttattta tgactgtttt gttattaatc actttaactg
     2521 tttctagtaa aaaaaatttc aatcaaaaaa taatttctca gccgggacat attgttgtaa
     2581 acaaagaaac agtagaggcg tataccacta aaggaatgga ttatttttta tatgattatt
     2641 ctcctatcgc ttcattggag aataaggagc atataataaa acacgaagca aagattggca
     2701 gtcaatgggt aaaccttcct tataaaacaa ctggtagttc tatcatatat agagtaacta
     2761 atgagaagag ccaagtaatc acacttcctg tatatgctta tttaggaacg aatgtagaaa
     2821 taaatcatca acctgtaaag gagcaggtga atgctaatgg attggtacaa ataagcggag
     2881 tcaaaggagt tgctgatatt aaagtaagca caagttatac agtaacgact attatttctt
     2941 atcttgttac attaactgca tatttatgtt tattttttaa aatagttttc cataaaaggt
     3001 aaattaactt ttcatttata aaataaattg attaaaaagg agaatctgtc gataaggatt
     3061 ctctcttttt tatacgaaaa atctgtaggg ggatgaagta atcgcttatt tcctcacttt
     3121 atgataaatt acagctaaag tcactagctt gtcttcttcg gaaaggagga gaatctatgg
     3181 atgaggaaca aatattcatt caaacacttt ataatctgat tttgaatccg aatacacgag
     3241 attgggaacg aaaagtattg attcagacaa agaatgacat aagagaaaat atatccgtta
     3301 aagaacagtt atcaaaacta gaagcaacac tacgtccatt agctatccga atgaatctga
     3361 cgcctgatgt aatggacttt tatttattga ttacggaagg atttgataag gagcaaaaat
     3421 atgacttttc caagcatgcc atgcaagatg ctgactatca agaacgtgca gtatttgcag
     3481 ggggatgttt ctggtgtatg gtcgagccgt ttgaatcgaa aaaagggatc ctatctgttt
     3541 tgtcaggata cacgggcgga catgttgaaa aaccaaatta tgatcaagta agtggtggtt
     3601 acacaggtca tgtggaagct gtggagatca tttatgacac aagagaaata agttatagcg
     3661 agttgctgac aatctattgg caaattactg atcccacaga tacatttgga caatttcaag
     3721 atagagggaa gcaatatcgt ccagtgattt tttatcaagt cgaacgacaa aaagaattag
     3781 cggaacaatc aaaacagaaa ctagacagtt caggaacttt ccaccaacca atcgtcacaa
     3841 aaatcgaacc agccggtacg ttttggccag cagaaaacta tcatcagcaa ttttacaaaa
     3901 aacaaccgaa acgttataaa aaaatccagc aagcacgtaa tcaatttctg atctatcaac
     3961 gagtaaaaaa caaatggcaa aaaaatatac gaaaaaatca ttttgattaa ctccttttct
     4021 ttaaaatgta gtaaactaaa aatagaaaaa agaaagcgtt ttaaaaagat ttgatgagga
     4081 ggagaaaaat tgtatattgc agatagtgca cgttatgaaa aaatggaata tcgtaaatcc
     4141 ggtcattcag gtttgaaatt accgatttta tctctcggat tatggcaaaa ttttggtgat
     4201 tatgatccta tccataatca aagagagatt ttaagaggcg cattcgacat gggaattacc
     4261 catttcgacc ttgcgaataa ttatggaggc cctgctggag ctgcagagaa aaactttggg
     4321 cgtattttta gagaagattt tcaagcctac cgtgatgaac tgattatttc tagcaaagca
     4381 ggttatcata tgtgggaagg gccatatggg gaatggggct ctcgtaaatc catcatttca
     4441 agttgtgacc aaagtctcca aagaatgggg ctagattatg ttgacatctt ctatcatcat
     4501 cgcccagacc ctgatacacc tttggaagaa acagcagaag ctttgatgca acttgtaaga
     4561 caaggaaaag cattatatat tggtatatcc aactataatg gagaagatac aaaaaaaatg
     4621 actgaaattt taaaaagaaa ggaagcaccg ttcattattc atcagatgcg ctacaatatg
     4681 ttttctcgtg cactactaga agacgatcta tctccagtat tagaagaaga agggctaggg
     4741 gcaatcacgt tcagtccgct tgctcaaggg ttgctgacaa atcgttatct acatggtatt
     4801 cctgaagatt ctagagcaca tcgtaaggaa ataccattcc tttcagaaga acaagttgga
     4861 tcaactctgg aaaaaatcaa agcactgcaa acaatcgccg tatcacgagg acaatcatta
     4921 gcgcaaatgg cccttgcatg gaatttgcga caaaaatctg ttacaagtgt actggtcgga
     4981 gcaagtcggt taagtcagtt acaagaaagt gttcgaatga tggataattt agatttctca
     5041 ccagaagaag aattgagaat cgaccaaatt ctagaataga aacgatgtaa tcaaactttt
     5101 tatccatata ttaaaaaaaa caaaagatga catcaagaat cgaatgaaga tcgtctgtta
     5161 ttgaacatca tattttttgt cgttcaaaat aaaaatgatc gaaggctagg agtgaatttt
     5221 atcctagtct tttttatggt aaatatcttc ttcgtttgta tgtctatgaa cggattaatc
     5281 aattttgtat acaaaaaatc atcagatgat tcaaacgtgt tataataatt acgaaaaaat
     5341 acatcagatg aaaaggaggt atgtggtttg gaagaaaata aaaccctagt agaagtaact
     5401 actgacaaga tccttagata tttaaaggaa atgcagttgc aagtaggaga taaactgccg
     5461 aatgaatatg aattgtccga ttatttagag gtggggcgaa gcacggtgcg agaagctgtg
     5521 cgggctctag cttccaggaa tatccttgaa gttcggcaag gagcggggac atttgtcagt
     5581 gataagcgag ggattgccaa tgacccactc ggtttttctt tgattgaaga tcaggatcgg
     5641 atgatacgag acttatttga actgagatat ttattggagc ctaagatggc aggtttggca
     5701 gcggaatatg caacgaagaa tcaaatcgtc caaatgaaaa aattaacgca aaagattgaa
     5761 acttcttttc gagaaggcac acaagaacat gttcaattag acatcgattt tcatacgatg
     5821 attgcagaag ctagcggaaa cgtagccttg ctgcatatta tcccaatcat taatgaatca
     5881 attgctttgt ttaataaaaa ttatgatatc cagcatctga aaatcgagac gatcgatatt
     5941 catacagaga tcgttcaggc catcgaaagg cggaatgcac ttgctgcgtt tgatgcaatg
     6001 actgtccata tggcaataaa tcggagagaa ttacagaaaa taataggaga aaaagaaact
     6061 aattgacagt tcatctgatg tattttatga taaatacatc agatgaattt ttgttttata
     6121 tttggtaaaa aagaggagta aatagtttct gttcatcgaa atgtaagcgc tgactaatag
     6181 acaaaaagag atgacgtcaa ggagaagaag caggaatgag gggatctgga atgagtttta
     6241 tggataaatt agccaatgtt ttagggaacg tggcaacgag aatcaataat ttaaggtata
     6301 tcatggtgat aaaaaacgca tttgctgcat tgattccggt aattattacg ggtgcttttg
     6361 gcacattgtt ttcagcaatg gtctttgact cggaaaatgg cttagcacag ataagtttct
     6421 tgcgtttttt agaagaatta aagcctatcg catctgccat cagctatgtc acactaagtt
     6481 tcttgaccat ctatgctgtt tttctgatag ggatcgaatt agccaagctg aataaagtgg
     6541 atggtgtatt ccctggtatc atcgcagtga tgtcttattt atctgttaat ccaactgttt
     6601 atgaattagt gaatgaaaat cagactgtta tagtagaaaa cgtattggct aaacaatata
     6661 cagatacgaa agggttattt ttgggaatgt tggtctccat cctttctatt gaactttatt
     6721 gctggttgag gaaacagaag aaactacagc tgagaatgcc tgataccgtg ccaacgaatg
     6781 tttctgctag tttttctgct ttattcccga cgattctgac tgtatcgggt atcgcggctg
     6841 cgggatttat catcaaagaa cttacaggaa tgtaccttta tgatatcatt tacaatgtcg
     6901 tacaacgccc attagaaggt gtcgtacaag ggttaccggg tattttgcta ttgatgttta
     6961 ttgcgcaagt cttttgggtg attggtatcc atggaaatca aatggtgaag ccgatacggg
     7021 aaccgttgct tttagcggca attgctgtaa atacggaagc ttttgaagct ggaaaagaag
     7081 taccgaacat tattactatg ccattttggg atatgtatat gagtatgggc gggtctggag
     7141 tcacgatcgg tttactgatt gcagtattgt tagttggaaa acgggaagat atgaaacaaa
     7201 tcaccaagtt atcccttgct cctgggatct tcaacatcaa tgaaccagtt atttttggta
     7261 tgccgatcat gctgaatcca attttagcta ttccgtttat cttaacgcct cttatcactg
     7321 gaacgattgg ttattttgca accgctttag gatttgcggc gaaagcagtt gtaatggtcc
     7381 cttggccaat gccgccgatc gtcaatgctt atctggcaac agcaggaaat atcggtgcag
     7441 ttattacgca acttgtttgt attattgttt ctattttgat ctatctgcca tttgtaaaag
     7501 tagcaaatcg tgtggaaaat gatgggatct aaaaaacagg aggaaatcat gaaaattact
     7561 attccaaaaa attttattct tggtgctgca tcttctgctt ggcaaacgga aggttggaaa
     7621 ggaaaaaaag aaggacagga ttcgtttata gatagctggt ataaaaatga acattttgtt
     7681 tggcataatg ggtatggccc agccgtagca actaatttta tggaacaata ccagcaggat
     7741 gtggatctga tgaaatccat ccatctgaca cattatcgga cttctatcaa ttgggctcgt
     7801 ttttttactg attatgagaa ctgtatagta gatgaagaat atgctcagca tataagtgaa
     7861 atgatcgact ctttgatcga agcagacgta gaaccgatga tttgtttgga acattatgaa
     7921 gtcccagctt ttcttttgga aaaatatgat ggctggtctt caagaaaagt agttgatttt
     7981 tatgctaagt atgctcaaat tgcctttgat cgttacggtg atagggtaaa aaactggttt
     8041 acattcaacg aaccaatcgt tccccaaacg cgttgctatt tggatgctgt tcgctggccg
     8101 catgaacaaa acacaaaaaa gtggatgatg tggaattatc ataaagtttt agcaacggct
     8161 aaagcagtag agatttaccg cagcggaaat tatggtggaa ggattggctg tattttaaat
     8221 cctgaaatgg tctatgctcg atccaattct ccagaagacc aaaaagctgc ttatatgtat
     8281 gatttgtttt ataatcgagt attttttgat ccgatggtaa aaggatacta cacagaagag
     8341 ttcctggagt tgtgtcaaaa acaatcgatt tatttcgatc ctgcaacaga ggatttagaa
     8401 acgattcagc atcatacagt agatttcctt ggaatcaatc aatattatcc taaacgcgtt
     8461 cgcgcccctc gttatcaatg gaatgaagag acacctttcc atcctgaacg atattatgaa
     8521 gtctttgatt tacctgggaa gaaaatgaat aattccaggg gatgggaaat ctatccgaaa
     8581 atcatgtatg atatagccat gtatctaaaa gaaaattatc ataatatccc ttggctgata
     8641 acagaaaacg gtatgggaag agaatatgaa gaacagtata tggatgagaa aggaatcgtg
     8701 caagatgatt accgtatcga atttatcgga cagcatttgc attacttgat caaagcaaca
     8761 gaagaaggcg caaattgcga aggctatatg ttatgggcat tcactgactg tgtgtcacca
     8821 atgaatgcat ttaagaatcg atatggatta gttcgaatcg aattgaacga tgaacgaagc
     8881 agaacattga aaaaatctgc ttcgtggtat cagactttga ttgataagag agaattggat
     8941 ttgcctgacg aaatctataa ataaatagaa gttttttgta gatgttcatt gtatttactg
     9001 gttatttgaa ataaaagagg ttgtgaatcc agcgttttga gctaataccc agggtcagct
     9061 gattgaacac cgtttattga gaggttgtga aaaagctgtc ctgcatcaag taataagaat
     9121 agcaaagtaa aaacaacatc ttatgttttt aacttggttg gacttattac cgcagatgca
     9181 agcttttgaa taccgtttat ttgaaaaaag ttgtgacaag ttttgtgtca caactttttt
     9241 tgtgcacgtt aaaagaaaaa aggtaataaa aattaatttg tgtgtttagt gtgcgctttc
     9301 tttttttagt gtgtatgcaa cttggtatag accttaaatg tgatgatatc aactttttaa
     9361 agcgctttta aagttggcac gctacttgct tatatacaag tgtaagtata gaaaggaacg
     9421 tgtttaaaat ggaaaagaaa acaatcatgc ttgtatgttc agctggaatg agtacaagtc
     9481 tcctagtcac aaaaatgcaa aaggcagcag aagcaaaagg aatggaagca gacatctttg
     9541 ctgtatctgc ttcagatgcg gataatcaac tagaatctaa aaatgtagac gttttacttt
     9601 taggaccgca agtacgcttt atgcaaaatc aatttgctga aaagctagca cctaaaggca
     9661 ttcctttaga tgtaatcaat atggctgact atggcatgat gaatggtgaa aaagtactcg
     9721 accaagcaga aaaactaata aacaaataat aaaaattatc ggaggataca ataaatggaa
     9781 gatcaaaaaa atctagaagc catcatgggt ctgatcatgt ttggcggaaa tgcaaaaagc
     9841 gatgcaatgg aagcgattgc tgctgcgaaa aaaggtgatt tcactttagc agatgaaaaa
     9901 atcaaagatg cagaggaatc tttagtccaa gcacatcact ctcaaacaga gatgctgaca
     9961 caagaagcac aaggaaatca cgttgaagtc acgcttttga cggtccacag tcaagatcat
    10021 ctgatgacat ctatagcttt tacagattta gcaaaagaga tcattgatct gtatcgtcga
    10081 ttgaacgcgt aatctacagt aaagaaaaga gggaagattt atggataaat ttgttgcatt
    10141 tatggagaaa cattttatac ctgtagcatc aaaaattggt gcacaacggc atttagttgc
    10201 gattcgggac tcatttatgg tgagtatgcc gttaatgatc ttaggagcat tagcagtaat
    10261 gatcaataac ttaccgattc ccgggttcca agaattgatg aactcaattt ttggtggtga
    10321 atcatggaaa ggattcgggg ctgctgcttg gaacggtaca tttgccattc tttctgtctt
    10381 gattgctttc ttgcttgctt atcatttagc taacggctac cgaaaagacg gtgtttccgc
    10441 aggtgtgatt tctttaggct ctttcttcgc tttaggcgga gcattaggta tgagctcaaa
    10501 cggtttgttt attgctatta ttgttgggat tatttcaaca gaaatctttg tacgtttaag
    10561 cggaaatgaa aaattgatca tcaaaatgcc tgatggtgtg ccaccggcag ttgcaaaagc
    10621 atttgcttca ttactaccag caatgatcac tatctctgcg tttgctttag ttgctgcaat
    10681 ttttgctgca ttcggtgttg atgatatcgt aggttctttc tatacagtgg tccaagaacc
    10741 atttatggga cttgcaaact cttatccatc tgccttactg cttgcattta tcacaccatt
    10801 cttatggttc tttggtttac acggagcgaa tatggtcgat cctttgatgc aaacaatcaa
    10861 tgcgccagcc attgaagcaa acgttaatgc aatcgcagct ggacatagcg caccgtttat
    10921 cgtgaacaaa cctttctttg acagctttgt caacttagga ggaacaggtg caactttagg
    10981 tttactgtta gctatttatt tagtaggacg taaaaacaaa ccatatatgg ttgtaacaaa
    11041 cttgtcgatt gcgccaggtg tcttcaatat taatgaacca acgatgtttg ggttacctat
    11101 cgtgttgaac ccgatcatgt tcattccatt tattttgaca ccaatggtct ta
//
LOCUS       DACDIG010000084        10832 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.084, whole genome shotgun sequence.
ACCESSION   DACDIG010000084 DACDIG010000000
VERSION     DACDIG010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.084"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..734
                     /locus_tag="IV247_002393"
     CDS             <1..734
                     /locus_tag="IV247_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP8778443.1"
                     /translation="YGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAI
                     DEIYHFDFLEAKELGLKHNSSFYSKHMDFSSSEPKTDLFFGATNNGRKERAEKYKQEF
                     TKRGVTTNYFILPLRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMY
                     FQKKLVTDNQAIAHYQFYHSENIFLLQERDLDELPEFLHTPFQPIPSELLEFFDAENW
                     AKRFFTTDSAIYEQYEYDLSSVKNK"
     gene            complement(877..1818)
                     /locus_tag="IV247_002394"
     CDS             complement(877..1818)
                     /locus_tag="IV247_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP8778444.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVVLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNKHVDLIVALTKANQLDDMFRSIQSIITENTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAEGM
                     VRTIVSEFASIAAKEGITLDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            1944..3065
                     /locus_tag="IV247_002395"
     CDS             1944..3065
                     /locus_tag="IV247_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP8778445.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTNWQGKNTAVIESAQVLGLDLLDKP
                     TDQTIRLASSYALGELIKDTLLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTRKLHLDRRNVLVSKVKVIIAADVTNPYTGQQGAAWQFGRQKGGTDTTLAFLD
                     KQAEKIAQQIKDVWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFELVSQITGLEKQI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDRELEGLFDFLPIV
                     LSIQLGPIDLEEAMEHRQTLEKTEILGQTLSRFFNILPK"
     gene            3323..4825
                     /gene="lsa"
                     /locus_tag="IV247_002396"
     CDS             3323..4825
                     /gene="lsa"
                     /locus_tag="IV247_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsa family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP8778446.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQAFVYFPQQTKDKERLTYYVLNEIMDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEIAKNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKKVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDALTMNSQVSHHKRLLSVENLQLGYENLLFEPVHF
                     TIEPHQRMAISGPNGAGKSSIIHYLLGTFNGKVVGEKSQPKHLTISYVRQNYEDNRGT
                     LAEFAKKNRVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYI
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLEKI"
     gene            4892..5419
                     /locus_tag="IV247_002397"
     CDS             4892..5419
                     /locus_tag="IV247_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP8778447.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQALAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVVDRLNLPMTADERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECMEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELISYE"
     gene            5631..6692
                     /locus_tag="IV247_002398"
     CDS             5631..6692
                     /locus_tag="IV247_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP8778448.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQNRLIELGSEAKKNKLELMVDISGEALKRAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPNDIDELKEAAADFSQIEAWHNYYPRLETGLDKEWYQEK
                     NQWLKTQGFLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSEHVQEQFASFQKEQTVLLHTEPVDEVFYEYILGKHTNRQDDARDVIRSADARF
                     REIPPIPVRNTAKRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            6695..7591
                     /gene="murQ"
                     /locus_tag="IV247_002399"
     CDS             6695..7591
                     /gene="murQ"
                     /locus_tag="IV247_002399"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP8778449.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELPSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISEHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEAAQAKLAEANGFVKGTL"
     gene            7605..9587
                     /locus_tag="IV247_002400"
     CDS             7605..9587
                     /locus_tag="IV247_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8778450.1"
                     /translation="MAKNELTLEELAKQIYQGAGGMGNVESIVHCMTRVRMKVINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQEMVDLAGVKLGEKLPSASATTAS
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAGTWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALATGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKAVPQASA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVISSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAADK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(9657..10103)
                     /gene="nrdI"
                     /locus_tag="IV247_002401"
     CDS             complement(9657..10103)
                     /gene="nrdI"
                     /locus_tag="IV247_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP8778451.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEYEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQRFGFPFLADYELRGTPSDVTRVYQILAEKNK"
     gene            10314..>10832
                     /locus_tag="IV247_002402"
     CDS             10314..>10832
                     /locus_tag="IV247_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8778452.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNVASIVMSTIFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVA"
BASE COUNT         3479 a         1922 c         2384 g         3047 t
ORIGIN      
        1 attatggatt tgatatggag atggctcgac gcgtgcgtcc atttaccaat gcaaaaatcg
       61 tgttattttt ttggaaccac tttaaagcag aacacgaggc gatgctgaag caggcacaag
      121 atgaaccggc aatcgatgag atttatcatt ttgatttttt agaagcgaaa gaacttggac
      181 taaaacataa tagttccttt tattcgaaac atatggattt ttcttctagc gaaccaaaga
      241 cggatctatt ttttggtgca acaaataacg gcagaaaaga acgggcagaa aaatataagc
      301 aggaatttac aaaaagaggt gttacgacca attactttat tcttccgctt cgaggaaatg
      361 aacaacctgg ataccttact tatgaagaat atctgaaaaa aaccggggaa tcgaggggga
      421 ttctagaact gttgcgggaa gggcagcaag gagtgacttt gcgaacgttt gaatctatgt
      481 attttcaaaa gaaactagtg acagataatc aagccatagc tcattatcag ttttatcatt
      541 ctgaaaatat ttttttgctt caagaacgtg atttagatga attaccggaa tttttgcata
      601 cgccatttca accaatccca tcagaactgt tggaattttt tgatgcagaa aattgggcaa
      661 aacgtttttt cactactgac tccgctattt atgaacaata tgaatacgat ctttcctctg
      721 ttaaaaataa ataaaagtgc caaccaatca tagatgaaaa taaacaagcg caattcttat
      781 ggcaacatga gaattgtgtt tttctttttg aactttcatg ggggtaaaaa aatgagaacc
      841 agctgctgat ccagctgatt ctcatcagag agccttttat tctgcgccaa ggattccttc
      901 ttttgcatgg actaattggg tcaaaaaggc acaatatggt gtagcaatat catatttttg
      961 tccttttcgc caaacggcac cattgatgta gtcgatttca gtcaaacgat ggtttttgat
     1021 caggtcttgg tacatagatg ggtaatgcaa cccgatgttg tcaggatcat aagtagaagc
     1081 aatatgttgg tacacttctt cttgatctaa tgtgattcct tcttttgctg cgatagaagc
     1141 aaactcagat acaatcgttc gaaccatgcc ctctgccgct ttttgcgcac ctaattcagc
     1201 aatattacaa tccaatatcg tgcataatcc atttaatgtt ccgtttacac aagcttttcg
     1261 ccaaatggaa taacgaacat tatgactata gattggattc aattttgctt cttgaaagac
     1321 ttctacaact ttttttgtaa atgcttcgcc ttctggttcc aagttctcta gttcgatttc
     1381 tccatcacct aataatgtga cttttccggg accagctaga ccagctgtcc acatcgtgat
     1441 cccaaataag atatttttct ttggtacata tttttccagt acatcttcat gacctaatcc
     1501 attcagtaaa cacaaaacat aagtattctc agtaataatg gattggatcg aacgaaacat
     1561 atcatctaat tgattcgctt ttgtcaaggc tacgatcaaa tccacgtgtt tgttgctttc
     1621 gatgatttct tctggtgaat agattggcag ttttgcaact acttctttgc cattaaagtc
     1681 agcaatcaat ccatttgtac gaatggcttc aatgtgagca ggccagcgat cgatcaagac
     1741 gacctcattt ccgctttgat gcaacattaa tccgattcgg ctgcccattg ctccggctcc
     1801 agctattgcg attttcatgt tttttctctc ctttattcaa tgagttccct tttagtatat
     1861 agaaaaacag aagaaaatag cattgttttg tttgaaaaaa gtgaaaaaaa gtgtcttact
     1921 taagaagaaa gtgagggata tagatggata ttttggcggc aatcgattca tttaaaggaa
     1981 gtgcttcttc atttgaatta aatcaggcag ttctaaatgg ttgggaagac cgtaaaggaa
     2041 gaaaaatcaa tgtgccgatt gcagatggcg gagaaaatac tgcagaagca atctattacg
     2101 cgctcggggg aacctggcgt acgattgatg ggatggatct cttatttcgt cagcgaaaaa
     2161 tcagttattt attgacgaac tggcaaggaa aaaataccgc agtcattgaa tctgcacaag
     2221 tattaggatt agatttactt gataaaccaa cggatcaaac catccgactt gcttctagtt
     2281 atgccttggg agagcttatc aaagatacac tgcttcaaga agtggaacaa atcattgtca
     2341 ctttaggagg aagcggatgt agcgatggtg gtctaggatt actccaatct ttgggtgctt
     2401 ctttagaagg gtacacagaa ggaaatcctt tgttatctac tagaaaactt catttggatc
     2461 gaaggaacgt tcttgtttct aaagtaaaag tgatcatcgc tgcagacgtg accaacccat
     2521 atacaggtca acaaggagct gcttggcaat ttggaagaca aaaaggagga acggatacga
     2581 cgttagcttt cttggataaa caagctgaaa agattgctca gcagataaaa gatgtttggg
     2641 aggtggattt agatgacatc ccaggaagcg gagcagcagg cggtattggc ggagcccttt
     2701 ttttgttagg agcgaagatg gtctctggtt ttgagttggt cagccaaatc actggattag
     2761 aaaaacagat cgaacaagcg gatgtcatca ttacaggaga agggcggatt gatcagcaaa
     2821 caatccacgg aaaagttcca tatggcgtag cagtgcttgc aaaaaaatat cgaaaaccaa
     2881 taatcgctat ttgcggaagt agagaccgag agctggaagg actctttgat tttctcccca
     2941 ttgtcttaag cattcaatta ggaccaatcg atttagagga agcaatggaa cacaggcaaa
     3001 cattagaaaa aacagaaatc ttaggacaga cactcagcag atttttcaac attctcccga
     3061 agtagttgac aaaaacaatt tctttatgct atcgtaattt caaatatagg acaaagtgag
     3121 ggatccgtgc attgaaaaat taagtcgatt gatgaaagct gcattcagac tgtaccttgt
     3181 ggagacaagg gagcagtgtg cagattttcg ggattggctt ggttctttcg atgtatgggt
     3241 ctttttgtgt gccccaaccc ttgcatcctc tttagccagt tctgaaaata ggactggtct
     3301 ttttttacga ggtgatggaa taatgtctaa aatcgaaata aaaaatctga cattcggcta
     3361 tgacagccaa ggcacattat tatttgaaca agccaatcta aactttgaca cacaatggaa
     3421 actaggactt atcggacgaa acggtcgagg aaagacaact ttactgaata tcctgcaaaa
     3481 caaactccct tatcaagggc aagtgatcca tcagcaagcg tttgtctatt tcccgcagca
     3541 aacaaaagat aaagagcgtt tgacctatta tgtgttaaat gagattatgg attttgagat
     3601 atgggaaatt gaaagagagc tccaactgat gcaaacagat cctgaaatct tatggagaga
     3661 attcagcaca ctatcaggag gagagaagac aaaagtccta ctggcactct tatttgtgga
     3721 tgacactcat ttcccgttaa tcgatgaacc aacgaatcat ttggatatct ctggcagaaa
     3781 acaagtagcg gcttacttga aaaagaaaaa acaaggattc atcgtggtga gtcatgatcg
     3841 gggatttatc gatgaagtag tggaccatgt tttagcaatc gaaaagagcc aactggaact
     3901 ttatcaaggg aatttctcta tctatgaaga acagaaaaaa cttcgtgatg aatttgaaat
     3961 agctaaaaat gaaaaattga aaaaagaagt cagcaggcta aagaaaacag cagctgaaaa
     4021 agcagaatgg tctcgttccc gagaagggga caaaacaaag aaaaaagtcg gattcatcga
     4081 tacggaatct aggcgagtga ataaaggagc agtaggcgct gatgctgcac ggacgatgaa
     4141 acgatctaaa gcaatcgtga atcggatgga aacccagatc agtgagaaag aaaaattatt
     4201 aaaagatatc gagtatatcg atgctttgac gatgaatagc caagtgtctc atcataaacg
     4261 gctgttaagc gtagaaaatc ttcaattagg gtatgaaaat ctgttattcg agccagttca
     4321 ttttacgatc gaacctcatc agcggatggc aatttcaggt cctaacggcg cgggaaagtc
     4381 atccattatc cattatcttc tggggacatt caacggcaag gttgtaggag aaaaatccca
     4441 gcccaaacat ttgaccatta gttacgtacg ccaaaattat gaagacaatc gagggacatt
     4501 ggcggaattc gcgaagaaaa accgagtaga ctatcaagca tttttgaata atctccgaaa
     4561 gcttgggatg gaaagagatg tttttcataa taaaatcgaa cagatgagta tgggacaacg
     4621 gaaaaaagtg gaattggcta aatctttatc ccaaccagct gagctctata tatgggatga
     4681 accattgaat tatttggatg tcttcaatca agaacaatta gaacaactga ttttgaacgt
     4741 gaaacctgct atgttactag tggaacatga tcaaaccttt ctagataaag tatctactga
     4801 gattatttct cttgagaaaa tctaaacctt gtaatctcaa agacaacaag gtatggttaa
     4861 ggagaacttt gtcaacgaag gagagaatca catgctttat gtaacgagac acggagaaac
     4921 aacttggaac gcacaaggat tagtttgcgg gcgagcagat gtgccgctga ctgaaaaagg
     4981 acagatgcag gcacaggcac tagcagaaaa ggtagtagat ttgcctgttc caatcacgaa
     5041 aatcatccat tcccctcttc aacgagcaag agatacagca caagcagtag ttgatcgtct
     5101 caatttgccg atgaccgctg atgagcgatt ggtagaaatg gattttgggg attatgacgg
     5161 actgccaagc aaagatgaaa actttcaaaa agcgcgttta gcgtttgctg tacgtttccc
     5221 aaatggagag tcggtcttag atgtgtacgc acggatcgtt cctttattga aagaatgcat
     5281 ggaagacgaa gaaaatgtct acttgttagt ctgtcataat gccttgataa gagtgatcaa
     5341 tgcttatttt catccaatgc ctaatgaagg gttctttaca ttcatggtag acaatacaga
     5401 attaatttct tatgagtaat tagacttttg tgttatttaa ataaaatgtt tcaaggcact
     5461 gcttttgctt agcagtgcct tttttgtggc tgtgtcaatt cttttggcat tataaatcca
     5521 atttttagaa ataaaattta attattttaa caaaagtatt gaaattttgt ttctttagat
     5581 atatactcta gtcatagaaa tgaagacgga tacagaaaaa gagggataat atgtttggct
     5641 tttcaatatt tatgaatgat tctttgtcag aagagaagaa acagtatatc cgccaaatgg
     5701 catccaatgg ctttgtcggt attttcactt ccatgcatat accggaagat gatgtttcgg
     5761 cttatcaaaa tcgattgatc gaactaggaa gcgaagcgaa aaagaataaa ctagagttga
     5821 tggtcgatat ttcaggagag gcattgaaac gagctggctt ttcattagaa gatctaacac
     5881 ccttgaaagc aattggtgta acagggttaa gaatggatta tcatatttct aatcagcaga
     5941 tcgccgactg gtcacatcaa ttgaaaatca gtttgaatgc cagcacgatc acgcctaatg
     6001 atatcgatga gttgaaagaa gcagcggctg atttctcaca gattgaagct tggcataatt
     6061 attatccacg tcttgaaaca gggttggaca aagaatggta tcaggaaaaa aatcaatggc
     6121 tcaaaactca aggattcctt gtacaaggct ttgtaccagg agatacagag ttgagaggtc
     6181 cactgtatca aggattacca actttggaag aacatcgagg gatccatcct ctggcaggtg
     6241 ctcttgatct attagcaagt aacacggata ttgtttatat cggagatgca ggtctgtcag
     6301 aacacgttca ggagcaattt gcttctttcc aaaaagaaca gacagtcttg ctccatacgg
     6361 aacctgtaga tgaagttttt tacgaatata tattaggaaa acatacgaat cggcaagatg
     6421 atgcaagaga tgtcatacga agcgcagatg cgcgttttcg agagattccg ccaatcccag
     6481 taagaaatac agctaagcgt atgaaaggat ctattacttt agataacgaa aagtatttgc
     6541 gctatatggg tgagatccaa ttaactaaat atgatttgcc agcggatgaa aaagtgaatg
     6601 tagtagccaa agtcatcaaa gaagaactac cgctgatcaa ccaaatcaaa gctggtatga
     6661 actatcaatt tataagaaaa gaaggaagat aaaaatgagc aaaatcaatt tagagaacct
     6721 aacaacggaa cgcagaaacc aagaaacttt tggattagat gaaatgagta tttctcaagc
     6781 agtccaactc atgaacaaag aagatcagaa agtggccgct gctgttgaaa aagaactgcc
     6841 aagtatcgag ccagtgatcg ccaacacgat tgaatccttt aaaaaaggtg gtcgcttgat
     6901 ttatatggga gctggaacga gtggtagact tggtgtatta gatgctgctg aatgtgtacc
     6961 gacattcggt gtagaaccgg aaatggtcat aggcctaatt gctggtggag aagaagcaat
     7021 gactgtagcc gtcgaaggag ccgaagattc ttatgaatta gggcaagaag atctaatcaa
     7081 gctgaattta acaaaaaatg atatagttat tggtatcgca gctagcggtc gtacaccata
     7141 tgtgattggt ggattagatt acgcaaaatc gattggtgct gttacaggaa cgatttcttg
     7201 caataaacaa gcagaaatca gcgagcatgc ggatttccca atcgaagtcg atgcaggacc
     7261 agaatttttg acaggctcta ctcgattgaa gtcaggaaca gcacaaaaac tgattttgaa
     7321 tatgatttct actatcagta tgatcggtat cggtaaagta ttcaataact tgatggtcga
     7381 cgtcaaacca acaaatgaaa aactaattga acgctcaaaa cgaatcatca tgcaagcgac
     7441 agaagctgac tacgaaacag ctgaaaagta ctttgaggaa gcagatcaaa atgtcaaact
     7501 ggctatcgtc atgattttga caaatagtga taaagaggca gctcaagcaa aattagctga
     7561 agcaaacggt tttgtaaaag gaaccttatg agggggaaaa gaccatggca aaaaacgaat
     7621 tgactttgga agaactggca aaacaaattt atcaaggagc aggcggtatg ggcaatgttg
     7681 aaagcattgt ccactgcatg actcgtgtac gtatgaaggt catcaatgat gatcttgtaa
     7741 atgtctcgga attaaaagcg attccaggag tacttggcgt agtagaggat gaacagctcc
     7801 aagtcatcat cggacctggg aaagtgaaca aagtagcgca ggaaatggtt gacttagcag
     7861 gcgtgaaatt aggagaaaaa cttccttctg ctagtgcaac tacagcaagt tcttctgcat
     7921 ctggaaaaga ccgtgtaaac gaacgggcac aagaaatgaa agcggcacaa aaatcgaaac
     7981 aaaaaccctc taaaatcaaa tcggtattaa aagacatctc taatatcttt gtaccaatga
     8041 tccctgcatt tgtcggcact gggatcgtgg cagggattgc cgcagtttta gcaaacttag
     8101 tcacagctgg aacactagac gcaggaacat ggcaacaata tatcgatgtg atgaacatct
     8161 tgaaaaacgg gatgtttagc tatctagtta tctatacagg tatcaatgct gcacaagttt
     8221 ttggtgcaac gccaacgctt ggtggagtta tcggggctgt tgtgatgctg acagggatga
     8281 atccagaagc accactgaaa aacttgttta ccggtgaagc tcttgcaact ggacaaggcg
     8341 ggatcttagg tgttatcttc gcagtttggt tattgtcaat cgttgagaaa aaactgcata
     8401 aagtagtacc tgatgcggta gatatcatcg ttacgccaac tctttccctg attgccatcg
     8461 gtctgatcga aattttcttc atcatgcctt tagcagggtt catttctgac ggcatggtcg
     8521 gtgggatcaa ctgggtactt ggtgttggag gagcatttgc cggcttcgtt ctaggtacat
     8581 tattcttgcc aatggtcatg ttcggactac atcagatctt gacgccaatc catgtacaaa
     8641 tgattgatga aactggtcgt accttattgc ttccaatcct tgcgatggcc ggagcaggac
     8701 aagtgggtgc agcattggca ttatggatcc gctgcaaaaa agacaaagaa ttaacagaaa
     8761 tgatcaaagg ggctttacca gtcggaatct taggaatcgg cgaaccattg atttacggag
     8821 taacattacc tctaggacgt ccatttatta ctgcttgtat cggtggaggt atcggtgggg
     8881 cagtgatcgg aatgatcgga aatgtcggag caatcgctat tggaccttca ggagccgctt
     8941 tgattccatt gatctctgat ggtaaatggt taggctatat ccttggttta ttggctgctt
     9001 acgctggtgg cttcgttgct acattcttct tcggtattcc taaagaccgt ttagaaaaag
     9061 aagaactagc gaccgaaaca gccacagtaa atgaagtacc tgaaaaagca gtacctcaag
     9121 cttcagctga aagtgtgact atcttatcat cagttgctga cggaacagtt gtttctttag
     9181 ataaagcaag tgacccagtt tttgcacaaa aaatgatggg tgaaggttac tttgtagaac
     9241 cagaaaacgg acatatctat tccccggtta ctggagtaat aagttctgtt ttcccaacaa
     9301 aacatgctat cggcattacg acaccaaatg gcttagaaat cttgcttcac atgggcatca
     9361 acacagttga cttaggcggt acgccattcg atgtgaaagt aacagaagga cagcaagtga
     9421 cacctgatac gcttgtagca gaagctgact tagaagcaat caaagcagca gacaaagaaa
     9481 cctccatgat cgtcttggta accaatatgg atcgtgtaaa aaactttgtc ttggaaaaaa
     9541 caggtaaagc gaaagcaaaa gcacctgtga tggatgtcaa agcttaataa gaatagaaac
     9601 aatagagaac ataaaaagaa gaagccgaaa tttatttttc agcttcttct tttgtgttat
     9661 ttattttttt ctgccaaaat ttgataaacg cgtgtcacat cagatggtgt tccccgtaat
     9721 tcataatctg ccaagaaagg aaagccgaat ctttgtgcgt attgtttggc tgtcagacaa
     9781 tattgattgt tgaagttttt attaccgcta ccgactacac caaggcaaag tttatgattg
     9841 tcttcgtatt ccagatattc gcgcatcgtt tcggtcaaga tttctgtgtc accgttgtct
     9901 aacccattcc cgccgtctaa ataagtagga acgaaagtaa aaaatggttc tgtttctttc
     9961 gtaaaatcac tattttcatg gatttctttt aaagtaactt caggattgac aggatcttct
    10021 gcatgcattt tttcagcata ttctgctaga tgtttggcaa aagcacgcgt atttcctgaa
    10081 atcgatatat ataaaatgtt catggatcat cccccaattc tttcccttta gtatagcatc
    10141 tttttaagtt tttttggtgg tgtaatttgg aaagggacta ggcgctttgc ttgtataatg
    10201 agaaaaaagt cggtagaatt atatttctat taaagttttt attaattaag taaaaataaa
    10261 aaaggtttgt tatacttttt gataaaagcg gttttacttt ggaggatgct tacatggata
    10321 cagagcaaca gaaaaaagga attggtttga ttccacttgc tgccttagtg attggttcag
    10381 cgattggagg tggcgtattt ggtattatga cggacatctc cggatcggct ggtggcccag
    10441 ctatcttcag ctgggtactc gtaggaacag caatgttgat gctctcctta tcgatcaata
    10501 atatcaataa aaaacgtcca gatttaaaag gcgggatctt tagttacgca gaagaaggtt
    10561 ttggccgttt cgctggtttt atcagcggtt ggggttactg gctcacatgc tggttaggaa
    10621 acgtggcgtt cgcaacattg ttgatgagtg cattaggtta tttcttcccc gtattcgaag
    10681 gtggtcaaaa tgtggcatcg atcgtgatga gtacgatctt cttatgggga tatgcatggc
    10741 tagtgaatcg tggagttgaa aatgctagct ttgtcaatgc tatcgtcaca ttttgtaaat
    10801 tagtgccatt atttttattt attgtagtag ct
//
LOCUS       DACDIG010000085        10702 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.085, whole genome shotgun sequence.
ACCESSION   DACDIG010000085 DACDIG010000000
VERSION     DACDIG010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.085"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(25..144)
                     /locus_tag="IV247_002403"
     CDS             complement(25..144)
                     /locus_tag="IV247_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP8778453.1"
                     /translation="MEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKGK"
     gene            complement(159..1343)
                     /locus_tag="IV247_002404"
     CDS             complement(159..1343)
                     /locus_tag="IV247_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP8778454.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(1599..1853)
                     /locus_tag="IV247_002405"
     CDS             complement(1599..1853)
                     /locus_tag="IV247_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778455.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            complement(1878..3020)
                     /locus_tag="IV247_002406"
     CDS             complement(1878..3020)
                     /locus_tag="IV247_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HAP8778456.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            complement(3080..4432)
                     /locus_tag="IV247_002407"
     CDS             complement(3080..4432)
                     /locus_tag="IV247_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP8778457.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFWVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            complement(4503..4847)
                     /locus_tag="IV247_002408"
     CDS             complement(4503..4847)
                     /locus_tag="IV247_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP8778458.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            complement(4857..5231)
                     /locus_tag="IV247_002409"
     CDS             complement(4857..5231)
                     /locus_tag="IV247_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8778459.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            complement(5244..5558)
                     /locus_tag="IV247_002410"
     CDS             complement(5244..5558)
                     /locus_tag="IV247_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8778460.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(5672..8899)
                     /gene="fss3"
                     /locus_tag="IV247_002411"
     CDS             complement(5672..8899)
                     /gene="fss3"
                     /locus_tag="IV247_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAP8778461.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITDAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            complement(8978..9640)
                     /locus_tag="IV247_002412"
     CDS             complement(8978..9640)
                     /locus_tag="IV247_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778462.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            10257..10487
                     /locus_tag="IV247_002413"
     CDS             10257..10487
                     /locus_tag="IV247_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778463.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENNQ
                     PVIAKKETVLSEGKIKTRYILEVETKFKNRSE"
BASE COUNT         2953 a         2365 c         1814 g         3570 t
ORIGIN      
        1 aaattttgac cgaaattcat ttcatcactt tcctttcttt tccattgctc ggtccgccgc
       61 tgcacttgca tgcatcaagc acatcacagt aacaccaagc atactgccac caaacaccaa
      121 taaaatatct ttcacaattt ccatcacgat tcctcttttc atataatcaa attttcaatt
      181 gccatacttt gttgctccaa aatctttttg tgccggtctg atagtcgagt attatttacc
      241 atatcctgaa tgtacgttgt ctgattcaac cgatccaact ccttggccat cttccacgta
      301 ggggcgactt gtcgtccaag ccagttcaac gttcgttcaa tggtataagg cttcggttca
      361 gaagttaatt gaatttcctt ctgattccgt ccaatgaagc gcccccattg ttcattcaag
      421 ggccagttag ttcgcctttt gccttccacc ttatccgcaa atcgcaagta ccggttaata
      481 atggaaaatg tggtactttc aatactgcga ccttttaaca aatcttgaat tgcatgataa
      541 gctcgatcat ttttcagtcg gatttcaaaa cgatttttcg tttcggtctg gtctaaagga
      601 atccctaact tgatatattg ctcatagtct ttttcgtaca aacagaaata gacctcactc
      661 ttaagactgc caatatacaa agtattgccc ataccatctt tatcatcagg tttcaacagt
      721 tccccggaac gatagctttt gaaggtacga aacaaggaga tacattcttc cttctgacat
      781 tttttcgtta aatcaggaat atctaacagg ccaactcgat cattgattgc caggtctaag
      841 cgtttcatca cgccaccggc ttttagacaa tcttcgaaaa agtcatacca gctacgcttt
      901 tgggcgagta aaaaagtttc aaactgccga caaccacgac cctttaattc taaaaggacg
      961 cctttatctt cttcatgtga caacatcacc acaatatctc ccataacata ttgttcttga
     1021 taggagtaaa aggcgtaatc ttcgtggagc atataatcaa atttcaaatg gagaatctct
     1081 tgaatgattt tcctcgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
     1141 gttaaaggta actcgggatt gaaactttcc agttggtcaa aaattttcct catggttttt
     1201 gtcgatgcgg ttacgccacc attttctatc tgatttaagt acgggcgact aagtccacaa
     1261 gtcgtagcta atttattttg tgaaacgcca tattcattac gccggtcttt taatagccga
     1321 taatctaaat tcctttgtgc caagttctca cctccaaatt taaggtgtta actttttcaa
     1381 atcagttaac atctacacaa attgcttatt atccttgata ggacagccat tccttatcgt
     1441 tgcttgctta ttttttgtgt ttttgtaccc ccctgttaga tacggggggt gagacatcat
     1501 ggccggctaa cgccgtccat aaacaggcac agcaccacgg aggatttctg cttcgcaaat
     1561 cccccgcggt cagtgcctat cagtggttta tttggccgtt actccgcggt aataccctca
     1621 atttccaact ctgcttttat ctcaaacaag tattggcgga tatcaggttc tttagcaaag
     1681 tccgcaattc gttccaattc actgatgact tgattcagct ccattcgagc atccgtttcc
     1741 tcatcaatta catacggatc atcagggact ttcaccccgt ttttgcgaat atcaaaattg
     1801 agccggtaca gttcttcttc caattccgga tctgactgaa tcttgccaat cacccagtag
     1861 attccccaat caactcatca taaatcatct gtttttacct cctttaatct tctagcaata
     1921 tcaaaaatga ccggaactgt tacagaattt ccagcctgct tatacaactg actatctgaa
     1981 ttgacttgag aagctcgatc aaaagcccag tccggaaagc cctgaagccg ccagcactcc
     2041 ctaggtgtta actttcgaat ttgatactga tccgtgacga ctgcttgttg gtctcctgtt
     2101 aataaggtat gcacactttg ttttcccaga cggcctcgac gacttgtgga attaggaaaa
     2161 gccaggttca cactgttccc tggcttcacc tcttgatatt ctgtttcgtt tttccgaagc
     2221 atgatcttcg ttccgccttg tttcgttgac aaagtaggcg ctgttccagt tatctcataa
     2281 acccgattgc cttgttcaaa atttcccagt aacatacccg ctacagaaat tccatcatct
     2341 accggcaatg cgattttgat tccttcatca ccggtcaagg taggcgctac gtcatctgac
     2401 ccatagactt gtccccccat tcctcgattg gaagggttga tatttaataa gtttctgatg
     2461 ttttgatcag atgttggaga cgatccaatg ataggaaata cttgttcggt acatgcgtct
     2521 cgagaatgtc cgataaggaa gatccgctca cggttttggg gaacgtaggc ctttgagtta
     2581 agcacttgcc attccacatc ataccccaat ccatccagcg ttcggaggat cgtctcgaac
     2641 gtagcccctc cttcgtgatt gagtaatccc cggacgttct caaggaataa aaacttaggt
     2701 tggagaatag cggcgaaccg agcgatttca aagaataaag tacctcgagt atcggaaaat
     2761 ccttgccgct ttcccgcaat tgaaaaagct tggcacggaa atccaccgca aaggatatcc
     2821 actggtccga gagattgaat aaactcgtct gatacacttg tgatgtcatg catttccacc
     2881 tccttacttg tatcatggat tgccttataa ctctggcgag caaaatcatc aatttcacaa
     2941 aagccgatac actggtgacc cgcttgttcc atacctaagc aaaagccacc aatcccagcg
     3001 aataaatcta aaaatgtcat aaatcacctc gtaaaaaaag cccaggattc ttgacactcc
     3061 taggctacat actattattt tatccttccg tattaacgtt taatccacca atgtcgctaa
     3121 tttcccttaa gaaatcatat ccttttggta caagtggcgt ataaaactca ctaatcacac
     3181 tccctcccgt atctacatag cctcgacctt tgatacgctt cataaagaat tttttatcaa
     3241 cctcgccaaa catcatggaa tatcccagtt cactcatacg acccaaagcc acccagaaat
     3301 taaattgatc gcgaatcccg tctcctaaat atttcgcatc cggtctttga caagctagaa
     3361 tcagaaagaa accagattga cgacctaaca tcacgatttg tttgagctta ttcaaaatca
     3421 ccgcactttc cttcgtcgtt aacatttcca tataaaccac gtattcatca aagattaaaa
     3481 agtttggtgg aagtccaaga aacgcataat tctctcctgt tttgtagttg ggcatttctt
     3541 tcattgcctt gctacgagtc atcatgcgct catagaaatc ttccacacaa gcagaaattt
     3601 cctccttttg ggaatagaca tgaggcatta ccgtacctaa atcggctaag tccgcatttt
     3661 tgggatcaag aacaaaaagc tcggcatcag attttaataa agcctcgatg atagtgagta
     3721 aaaagtaagt cttcccacca cctgtgccgc cagcgattaa catatgaggt aaagaatcat
     3781 aagcccatac ttgatttttc atcaatcgca aagccccact ttctgccact acttcgtcta
     3841 tcccaattcg attggcaatt atatcataca gtaaagtgta ctccacatag gagtctttga
     3901 gttccttttc tactagttca caatatagac cactttctaa cttcttttcc agctttaaaa
     3961 gctgatcttg atattttccc agtgaaattt gaacgcgaat agaaagtaaa ccatccttca
     4021 tacggtaata gattttgggg aagtaactga ttgtctcttt ggttcgacta ctattgaggt
     4081 ctttgaaaaa tccttcactc tgagtttgtt ttacttcgta ccaatgattt tctagaatca
     4141 tccgggctag cttttgtcga tgccacaaac tgcgccaatg atcctgaaaa aagtgtatat
     4201 aaagcaacat cgtgcttcct gaaagaacga gacttcctag caaatagatt aaatctttta
     4261 tagaacgaac gatttctata gtagaaagtg atagcttaga aatatcaaat tgtctaagct
     4321 cttgaaaata aaagacgctc atccaaccga tgaacagagc gagtagccag ccaagaacaa
     4381 agtgataaac caagtgctga tctcctgccc gtatacgatg ccctttgtac atttttatgg
     4441 tttccacctc caatcgaaaa aagcttcata ggatttcatt gatccaattt caagcagtta
     4501 tgctagtcat ttactgctat agggagttgt ttcgtttcca ctagttgctt taaaaaaact
     4561 tcaatggcat ccggcatttc cacaccaatt tcttccaata cggtttcggc ttctcgacat
     4621 agactttcat ttacttcgat aaccataaag aaggactctt cttcccctaa ccaattcagt
     4681 acatcaaaat cttcttcggc atcgttacct acttccagta acccttgatg cataatcaga
     4741 aacatttttt catcataggt taatgcttta gtgagttgtt cctccgttag ctcacctttt
     4801 ccatattgat cgagacaatc taaaatatgt tcaacattaa tttccatacg cttcacctat
     4861 ttctttcctt cattttggtt tgtagaattt ccctgatctg gttgtcgttt caagactaaa
     4921 tcatcggcct taatgaacca attgacagta gttccttctc ggaaaacata attggcgacc
     4981 gtatccatga ttggattgac caactctacc tctgcgtcat aggcaaactc gcgtaagggt
     5041 acatttgctg ggatactaac ttggatcatt cgaccttgcg cagaggattt caaattataa
     5101 gtccgttctt ttacctcgtt actaaccgtt ccatcctcat tttgcttccg tacttctcgg
     5161 cgtaatgcgg aaaatcgtaa tttcccaaac gttagtcctg aatctacaac aataccttct
     5221 gttaatctca tcttcttttc ctcctatgct ttcactaaat catcgacata caacacgtag
     5281 tcggtaaacc cacgattctc aatctcatag cctttggcaa ccaaatgggg attcacggct
     5341 tttagtggtt gatcttgatc aaaatccttt tcgccggctt ccgcagctac caccacttca
     5401 atatcatcgg ctcgttgctt actggaatac aaatgataac tacgaccaat tactgtagta
     5461 ttccgcccat atccttcagt taaaacttcg ccttcgccgg cataccttat ctttccaaag
     5521 gtttcggcca tatctggcac aacaaatttc aattccatct tcttttcctc ttttcatttt
     5581 tagttttgga tacaaaaata gcgcgaccac tttttttagg aggcggtcgc gcacgttggt
     5641 ttttcctatt tagttgtttc atcacatcac cttagtttcg gcgattccag aaataatacc
     5701 cagcagtcgc aaagatcaag gtaaacccaa taaacagcag aacattagaa tttttctccc
     5761 ctgtttgtgg gaatgtcttt actgtaggac tagattcttg agagtcactt ggaacagttg
     5821 gtatttccgg ttgttctggt gcacttggtg ttgtcggaac ttttttaggt gttaaagttt
     5881 gagttttttc ttttagatcc tcattgtgtt tcccattgat gtttccgtct ttgccatagt
     5941 taatttcttt gaaggtaaaa cttgtacctt ctggatattt acttgtgtcc actttttccg
     6001 caagtacggt tttagtgaat tctttgtcat tcacttcata atcaattttg ccagatttcc
     6061 aaatttcttt ggtcgtatta tccggaagta atgcatacag aattgtttca aaagcttctt
     6121 ttgagccatc cagtacatca tgggtaatcg acacatcatc gaacatatcc accacatccc
     6181 catgagtaaa ggtttgcgaa ccatcttcta ggtgggcttt tgtttggatg gaaacatggc
     6241 gttccaccgt acaattgaca gtttgggctt gattgttcaa gctagcatct ttcgctactg
     6301 gatcatcacc tgcttcatag ggtttcttgt tttcatacac atagttgaat aagacgatgg
     6361 ttttatccaa aacttgttct gcggttaatt tatgcaaaat tttccaagtt cctgttttct
     6421 ctttattcgc caacgttgcg gttgtttcgg caattaccac tggtttggct ttttcatctt
     6481 tttcttgggc agcttttgtc gcttccacat caatggcttg cgctaccaca taccagtctt
     6541 ctttgatttc atgcaagtta tagctcaatt tatcaactag ctcggtagaa tcaaggtctg
     6601 tcaattcttt gcctccgtct ttccaagtcc caagagaagt taagctatct tcttcttcag
     6661 gtaaatcata gagcatcaaa cgattcaagc taacagaaaa tgctttattc gtcagtttct
     6721 cgtaaggaac ggtcaccgtt ttaattggtt gtttttgtcc tttttcggta atcgtaaaaa
     6781 tgtattcact cttagtgtaa tccgctttat tttctttaaa agtagaacgg atttctaatg
     6841 gcgtaatttt ttgaaatcct tctggagcct ctacttcttc aagtaaataa tccccataag
     6901 gaagattttc aaacttgcca tagccatcaa aacctagttg ctcgttataa gctgtaattg
     6961 cttcatcttc ggcatctgta atctcactgg ttccttccaa tggcgatact ttaaaggtca
     7021 gatcattgaa gccagtttcg gcagtcccag cagctgatcc agcaaattta aagaaatcaa
     7081 agccaaagcg aatgacttgt tcttttgccg taacatctcg ggtaatcacg gcattttttt
     7141 cgttatcatc cacctttttg atggaaacag ggtactttgt ttcatccaag gtatatcctt
     7201 caggtgcttt ggtttcttgc cagtaatact cattaatcgc taagtgttta acagcaactt
     7261 gattcttttc atctaaagtc aaagtcaccg tttcatcaga agctttcgtt cccttcacta
     7321 actctggttt aaaagcctca ctccatttga ccgcttgacc atcttttgca gtaaagagag
     7381 tatattcagc tcctttaaac tcagctttac cttgagtttc attaccggta tctttgtctt
     7441 cttttgtcaa agtggtttca ccagtgattt cttggttttg cccttttacg ttactggtaa
     7501 caagagccac ggtttgattg acatagttca attcgacttt tactggttta aaggtattca
     7561 caaagccatt actagctttg gtttcggtca cataataggt tcccaattcc aacgcattgg
     7621 caatctcttt tggtgtttcc gcatgacctt tttcatccgt ggtcatttct tggacaattt
     7681 caccggttgg actgtctttg cgaatggcaa acgtatttcc ggctaatgag tatttgtcgt
     7741 tccaaagatc agttcctgtt tccaccccca ttttattcaa aatgatttga cctttttcgc
     7801 gagtattttt tgaagtcaca gaaatagtct caccagattt aatggttgcg gtgattggtg
     7861 tagtgtcaat cgtataagga gctggcactg atttttcagt aatggttact ttcgcgccat
     7921 gaggaatctc atccaatgtc gcaatgcctt ctttatcggt ggtcacgtct tttgcaggta
     7981 atgtttttcc aaagtctaaa tggaaaacgg ttcccggtac aacatttcct gtttctttat
     8041 cgactttttt aatcttcaaa gaaccctcag tttccacatc aattttgatt gtgtaggtat
     8101 tgggcttatc aattgccccc gccatcaaag tttgttgacc agccatttta taagctaccg
     8161 gtgttcctgt attagctgac ttctttaggg ttaattcacc actctctttt gaattggcat
     8221 ttggagtaat cactagttga ttgccgttca cacgataatc aatatttgcg atattctcga
     8281 caacttcatc aaactccgat aaattcaagc cgttcgtatc tgtcacagta gtcgattgac
     8341 ctaacacggt tttcactgtg gagttatgga agcttggttt cttttgataa tccgcaatgg
     8401 cttgattgat ttttgactca atggctgcga tatttactgc actaccatca gaacgtttaa
     8461 ttgagtgaag cgtgtagcca ttaacttctt gccaaatcat cttctgggcc acaatatgtg
     8521 tatccacatc cgtaccagct tccttccata aaacggaaac taatttcgct ttctccgaca
     8581 tatcaggcaa tggatttttc tcataaccag gagtgaactc attgtaaatc ggcacccctg
     8641 gctcaataca gaaaatatct tgcttcacac cattgtaaac tgcatagtgc ggaacggtaa
     8701 cttctgacat gggacgacca tcgctaaatg taccttcgac agcatactgg tgagccaaat
     8761 ccaattccac agttaccgtt tgtggatgag taatttcatc agcaaaggca atcgttgggc
     8821 caagcgatcc accaattgtt tggcataata gactcagtgt tgcgagcaaa cgccaattct
     8881 taaatcttgg ctttttcata aaataaatct cctttcgttt ttgggtaata aaaaagacgt
     8941 ccaaatttta attttgaacg tctgatttag agtgatacta tttagttttc tttgtcttgg
     9001 ataggttgtt tctctgctta taaattttga ttcttcgata atcaaatcca atctctgcca
     9061 tcaattcctc ccaatttttg ttcgttcttt tcataattgt atcaactgat ggtagttttg
     9121 gggattgaac tagttttcga aactttgtca ttgataaatc atcctgtagt tttaatcgga
     9181 ttatttcatt ttttagttct aacagcagtt cgaaatcagt aaatcgagat ccatcatttt
     9241 tttctttaaa aagtggacgt atatctccta acatcttttt aatggtacta agtgaaggaa
     9301 tattcttccc tactgatttt tgttcgtaca ttcgttgaga ggttatcttc tcgcacttaa
     9361 taaaggcttc tactatttcc aataattctt gttctgacat tctagcccat tttccatacc
     9421 cagtattttc tcgtcctata ctaatcaata aattctccca tgatccatat tttttgttaa
     9481 tgaaccacat acttggtgca gaatttggat tttctgatag ctttttctgg tattgacggc
     9541 tagatgtaat tccgtgtttt tcgatgaatt cattaattgt taaaatcagt tttaactcac
     9601 tttgattttt ccaaatgata cgtccatcat tttgcttcac gataaaaact cctaatagtt
     9661 atttagatag aagcctatca catcaacttt ttagtagaaa agtacatgtt ttgtcttttt
     9721 tgctaatagc tgaccctttg agaaacttgg gttcaaaagt tgctaaattc ggtaaatgtt
     9781 ttggtatttt tcgtattata tttgtcgtca agctgactac taaattttgc cctattttga
     9841 cgacactgat agtcaatgtc ggacaatcta ggacaatttt taagggaata tgattgaaaa
     9901 atggtccaaa acagccaaaa atggcttaaa actgtgatag agaaaaaaga aaaagccctt
     9961 gatatacaag gacttttcac aaatttcaga tttccaagga agttttccgt gtttatcttc
    10021 tgatttcttt gatacgagca gcttttccgt gtaatgcacg taaacaaaac cactatattc
    10081 tattatgtat ctaataccct caaaacagct ataaatcaac aataccgaca tataataaac
    10141 actatgttca atctgagata tatcaaaggt aaacgtattt ttgccccctt tttgcccctc
    10201 aaatatcgct ttacaacgag aacgtaagtt cgtataatcg ttttgaggtg atttttatga
    10261 tggaagactt tattagaaaa aatatcagtg atgaatatgc agacttttat gagcaaagca
    10321 gcaaaaaaga taaattccag atggatgttt caatcttagc tatattagct ttttccgaaa
    10381 ataaccaacc tgtaattgcg aaaaaagaaa cagtattatc tgaaggcaaa ataaaaactc
    10441 gatatatatt agaggtagaa actaagttta aaaatagatc ggagtaatgg ctatgctttt
    10501 taatgagaca caattatggt ttaaatttga tccttcgaat agatttatca aagattttta
    10561 taaggtgtgg gattcagaag ttttctttct agcaatcgaa gatagcttat taatcaatct
    10621 ctactattct aataagaact actttaaaat ccctgctgca aaaactagaa tgaagaaaga
    10681 tgtatacttt ttgtttgata tc
//
LOCUS       DACDIG010000086        10414 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.086, whole genome shotgun sequence.
ACCESSION   DACDIG010000086 DACDIG010000000
VERSION     DACDIG010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.086"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..177
                     /locus_tag="IV247_002414"
     CDS             <1..177
                     /locus_tag="IV247_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778464.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLNLFNRIVGA"
     gene            190..690
                     /locus_tag="IV247_002415"
     CDS             190..690
                     /locus_tag="IV247_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP8778465.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            787..2634
                     /locus_tag="IV247_002416"
     CDS             787..2634
                     /locus_tag="IV247_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HAP8778466.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2637..3533
                     /locus_tag="IV247_002417"
     CDS             2637..3533
                     /locus_tag="IV247_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8778467.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNFYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3583..3972
                     /locus_tag="IV247_002418"
     CDS             3583..3972
                     /locus_tag="IV247_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8778468.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3959..5461
                     /locus_tag="IV247_002419"
                     /pseudo
     CDS             3959..5461
                     /locus_tag="IV247_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
     gene            6206..8032
                     /gene="ltrA"
                     /locus_tag="IV247_002420"
     CDS             6206..8032
                     /gene="ltrA"
                     /locus_tag="IV247_002420"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP8778469.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
     gene            8145..8984
                     /locus_tag="IV247_002421"
                     /pseudo
     CDS             8145..8984
                     /locus_tag="IV247_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343921.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
     gene            9077..10264
                     /locus_tag="IV247_002422"
     CDS             9077..10264
                     /locus_tag="IV247_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAP8778470.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT         3536 a         1654 c         2253 g         2971 t
ORIGIN      
        1 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
       61 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
      121 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
      181 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
      241 gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa agaacggatt
      301 ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc ttttgagatt
      361 gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga agagcttaaa
      421 ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa ttctttcgag
      481 gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga catgagtgat
      541 gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa ttatttggtt
      661 acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc agaaatttct
      721 atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt gataggggtg
      781 ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc aactgcgagt
      841 cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg tttaaacaaa
      901 ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga tgaatataag
      961 aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac gtgtggagaa
     1021 aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga tatctctgat
     1081 ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc ttgctcaatg
     1141 aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt tattcctgca
     1201 catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg aaagatattt
     1261 gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga aaatcgctca
     1321 caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc cagatatgat
     1381 ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga ggcagaaaaa
     1441 aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa ttcaaaacat
     1501 ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga acctgacttg
     1561 ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga tttttctaat
     1621 ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg gaccactctt
     1681 agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat tggaggaaga
     1741 ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa ggaagcagaa
     1801 cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat tgaacataat
     1861 ggctctggaa tatttttgaa ggatacaatt gtgaaactga atattttcta taaggggaca
     1921 aaatatcaaa taataagaac acaggataga cattctattt atgaatttac acaagagaat
     1981 ggtctggttg aaaaagaaac agaacggttg agaatgattt ccgataaggt gagtatatac
     2041 tctcaaaaac aaatttatga aatttcaaaa aatcaaacta gtatactaga attactagat
     2101 ggatataact ctgatttgat ttcagagtat aaaaatgaga ttgaaacatg tgtaaacgaa
     2161 ataagaaaat taaattggga tattgtttct gtgaaaaaag aaatacaaga taaggcgaag
     2221 gtagagcttg aaattgaaga tttaaaaaaa caagtagaaa agctatctca taagagttat
     2281 aaagatgttt atgatgagta ttcaaaagag acagatattt atagggaact gaaacgtgat
     2341 gtggaggcac ttaaggaaat tcccaaaaat ctaactgata gtgttgacaa aattgacttt
     2401 ggcaatggta taaaagttac agacgaaatt gatgaacacc gcggagaatt gattaagaat
     2461 ttagtgacca atcgggctaa gattcaaaca ataattaatg aaatgtcaga tgaaattgat
     2521 agttatagag cagagctgaa taaatcagat tggaaagtta actataatga ggtttcaaag
     2581 aggtatcaaa gaggtatcaa aaacttagtg aagagttatc tcaagatgaa ttaaaaatgt
     2641 tacaaaacct tgattcttta tatcaatctt tatcaaaaac tgaagagcga ttggaagaaa
     2701 ttattcaaca agaaataaag cttaaagatt accaagttga ggttgactca gcaatgtcaa
     2761 gaatgaataa atcatatcaa aatttctatt ctgagagaag tagttcctcg cgcgaaattt
     2821 tttctgatat tacaggaata gaggctacta ttcaaccaaa tagggatttc aataattatg
     2881 ttaaagagct cagagcttgt attaataagg aggacacttt tgacagtgag tttggatctc
     2941 ttattgataa gctgacaaaa aattccatta ctaatcaaga tatatataat gacatacttg
     3001 atataagagt tagtggggaa agtagtattt tttcagataa aaaattaatc aattctataa
     3061 gaaaattgaa tgatgcacaa gttcttgaat tacaaaccct taagccaaat gatttagtag
     3121 ttatccgact tgaactaaat ggaaaaaaag ttagtttatc gaatgctagt gctggtcaaa
     3181 agacatctgc aattctttct atgattttgg cgtatggaaa ttcaccactc atattggatc
     3241 agcctgaaga tgatttggat agtcaattaa ttaataactt aatcgtaaga agtatcatta
     3301 caaaaaagga aaacagacaa attttaattg taactcataa tgcaaatatc ccagttaatg
     3361 gtgattcaga atggttgatt agcatgggag attcaaaaga aatatcggct gatcagtgcg
     3421 ggtcggtaga tgaacccaat atcaagccaa gaatatgtac tgtgatggaa ggtggcgagg
     3481 atgcttttac aaatagagcg aaacgctatg gttttaaaca aattgtagaa taacactaga
     3541 gggtgagaca tattattatg tccgcccttt ttaggaggaa aaatgaaaaa aataaaaagc
     3601 tataccagta tctgggcagt tgaaaaagtc atttacgcaa tcaatgattt tcagttgcct
     3661 tttcctgtga cttttaacca aatggcttgg ttcgttttat ctttactgtt tgtcatcgta
     3721 tttgcccatg tgcctccact atctatgata gaggaggtct ttttaaaata cttaggcata
     3781 ccggtggctg tcacttggtt tatgtcgcaa aagacgtttg atggcaagaa accactggga
     3841 tttttacgat cctttatcag ctatcacttg cgatttaagg tgacttttgc aggaaagaaa
     3901 gtcaaagaac agaagaaacg atgggatgaa cccagtactc ttgtaaggag tgtgaactat
     3961 gtatccgatt aagtatattg aaaataatct cgtctttaac caagaaggcg agtgttttgc
     4021 ctactatgaa ttagtgcctt acaattactc ctttttgtca cccgaacaga aatttcaagt
     4081 gcatgataac tttcgccaac taattgcgca aaatcgtgaa ggcaagatcc atgcgttaca
     4141 aattgccaca gaaagtagta ttcgcgccac acaagaacga tcaaaaaaag aaattaccgg
     4201 tagactcaaa gaagtagcga aacaacgaat tgatatacag acagatgcat tagtatccat
     4261 gattggcgat agtcagattg attatcgctt ttttattggc tttaaactaa ttgcgacaga
     4321 tgaagaagtc aatctaaaga gtctcaagaa atctttcttt tctgggcttc aagaatttgt
     4381 ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg aagaaattcg
     4441 ctgctattct aagcttgaaa aattaatgga aagcaaattg gctcgaagat ttaaagtcag
     4501 gcgagtcaca ccgagtgacc tgacgtattt gattgaacat atttatggcg aaaaagggat
     4561 accgtttgaa gaatacgaat ttcagctacc aaagaaaaag ctaaaatcag aaacactggt
     4621 gaaacgttat gacttattac gtccaaatcg ttgtttaatt gaagaaaaac caaggtgctt
     4681 acggatggaa catgagaatc atgagtcgta cgtagcgtat ttaacaatta atacaattgt
     4741 gggggagatg gaatttcctt cctcagaact tttctattac cagcaacaac aatttacatt
     4801 cccaatcgac acaagtatga atgtagagat tgtgacgaat aaaaaggctt tagctaccgt
     4861 tcgtaataag aaaaaagaac tgaaagattt agacaatcat gcttaccaat ctgacaatga
     4921 aacgaattcc aatgtcttag atgctttgga ttccgtggat gaattggaaa ccaccttgga
     4981 tcagtcaaaa gaatctatgt acaaactgag ttatgtggtt cgagtgagtg ccgaatcagt
     5041 ggatgaacta aagcgacgct gtgatgaagt gctcgatttt tatgatgata ccaatgtaaa
     5101 actagtccgg ccctttggcg atatgatggg gctacatgaa gaattcttac ccttaagtaa
     5161 acgatatatg aacgactata ttcagtatgt tacgtctgat tttttggcgg ggcttggttt
     5221 tggggctact cagatgctag gagaattaga agggatttac tttggctaca atgtggatac
     5281 aggtcgtaat gtttacctga aaccggcatt ggcttcacaa ggagtcaagg gttcagtaac
     5341 caatgcgctg gccgccgctt tcctgggttc tcttggtgga ggtaaatcct ttagtaacaa
     5401 tctcttagtc tactatgctg ttttgttcgg tggtcaagct gtgattgtcg atccaaaagg
     5461 gggtgcgaaa cgttcctact tgaaaagagt aggcacagca cttcattaaa tcaatattta
     5521 atgtgtgttg gagaactaaa cacacgaata gtcgaatgac ggggtaacgc cctgaaaggc
     5581 tactttaata ctctgactgg cagaaacttg agtaaacaag gctctgtcag aagctcggtg
     5641 aagtcggcag acagacaccg taacagataa tgctgacgaa agattcccag aggtgggcgt
     5701 tgtgtcctat atgccggaag tctataaaat atctatggtt agaatgtaga tgagaggtct
     5761 actgacgaat ccttgaatgt acaggtctat atcggggctg gatagaaatg tccagtgaca
     5821 caaacagtgt cgctactgtg ggtgagtaag actccgcatt tatgaaaatc catataacgt
     5881 taaaggcgtt atcaaggtag caggcttata gaggaaacct aaggatatat gtacagatag
     5941 tgtgtttgga acgttgaaag ctgacaacat ggagatttta ctgtctgtga agtgttcaca
     6001 tcagaaagtt attgtgagat tagcaatgat ataaggtgtg ttaatgtcag tgagagtggt
     6061 ggcacagtac ctatgaaacc gtgataacaa gcggtggagg gatagccact agtcatatga
     6121 agaatagaat tactaagaaa aatggtatgg gttcgagtaa gactaagaga tgtacttgct
     6181 cctgcaaaca aggagggtac agaccatgct aaagaaaaac aagctccgct ataacgagta
     6241 ttatgatatg caacatattt atgacgagtt atacgcacag agcaagaacg gtaataactt
     6301 ttataagtta cttgaaatta ttggttcgga acaaaatatt tgtttagctt accgtaatct
     6361 gaaatcaaac agtggcagta aaacggctgg aacggatgga atgacgatag acgatataaa
     6421 acaactcagc aatgctgaga ttgttgctac cgttcgggaa agccttagca actaccgccc
     6481 caaatcagtc agacgtgttt tcattccaaa agctgggagc gataaaatgc gtccattggg
     6541 gattccgtgt atctgggaca ggctggtaca acaatgtatc ctgcaagtcc tagagccgat
     6601 atgcgaacca aagttccata atcattccta tggattccgt gcgaatcgaa gtgcccacca
     6661 tgcagtcagc agagtaacga cactgataaa tttatcaaaa tatcattact gtgttgacgt
     6721 agacattaaa ggatttttcg ataatgtcaa tcatggaaaa ctattaaaac aaatctggac
     6781 acttggtatt cgggataaaa gattgatttg cattatcagc aaaatgctga aagccgaaat
     6841 tgacggcgag ggtgtcccag aaaaaggaac gccacaaggc ggtctgttaa gtccactgct
     6901 ctccctcata gtccttaatg aattagactg gtgggtaagc agtcagtggg aaacattcca
     6961 gccgaagaac cgcagtaaaa atgggtggct tcaatacgca aagaaatata ccaaactgaa
     7021 aagcggtttt attgtacgtt acgctgatga ttttaaaatc atgtgttcaa cctatggaga
     7081 agcacaaaga ttttaccaca gtaccgtaga ttttcttaat aaaaggctga aattagaaat
     7141 cagtccagag aaatcaaagg tagtaaatct gaagaaaaat tcatcagatt ttctggggtt
     7201 caaaatcaaa gtcattccaa aaggaagaac gaaacacggt tatgttgcca aaacagatat
     7261 gaaccaaaaa gcattaaaga aagcgaaaac aaatctaaag ttaaaggtaa aagatatagc
     7321 cagacatacg actggcttca atatcagcag atataatctg acagttatcg gtatgcagaa
     7381 ttactactgc atagcaacta acgtatataa caacctgaca gaagtaagtt acgctctctt
     7441 accgaccata cgaatccgtt taaggaacat tgcaaaatca gttccatttg aaagtacaag
     7501 ttcagaattt cagagcagga caaaaggaat cagaccaaaa acaaaaatag tgatgattgc
     7561 ggataatccg ctattgccga ttcaaggagt acaacacaaa aatcctatga atttctcgca
     7621 agatatttgt aattttacaa agcaagggag aaacaaggtg catgaggacg ttgtagttgt
     7681 aacaaaagaa gaaatccgag cgttgttgga aaatgaaaac cctgccgata gcgtagagtt
     7741 taacgacaat cgtatttcgg catatatcgc acaacaaggc aactgctatg taatgaatcg
     7801 tcgaggaact ccgtccactc tgatatgtat tcacaaatca gacaaaggag ataaccttga
     7861 cagatattct aatctggcat ttgtggaaat tcccattgca aaggcaatcc taacagaatc
     7921 agcagacgaa gcaaaatcac tgttaaaagg ttatgttctt aatagtcagc agaagaaaaa
     7981 actgaacaga atacggacga attacggata ccagacaata actgggaaat aacttataaa
     8041 tctatttgga tatgatggaa cgccgtatga agcgaaagtt tcatgtacgg tgtgaagcgg
     8101 gggaaaactt ggagataaca tcaaaaagtt acctatcgct atagcgagga gggtggaaag
     8161 aaaccttgcc agaaattgcg gatgaaatca gtatcctcaa cttgacgagt aagtcagaaa
     8221 atcaaggtct gcttgatcct tatgtgatta tgaagaaaaa gaaagactcg gaaagtttag
     8281 caattgatat tctgactttt ttaacaggaa tttcaagtcg agacggggag aagtttcctg
     8341 tgttacgtaa agccattcga acggtgactc aaagtgatca acgggggtta ttacaagtta
     8401 ttaacgaatt gcgaaaagac gggtcaccaa tcgcggaaaa tatcgccgat catatcgagt
     8461 cctttaccga ttatgatttt gctcatttgc ttttctcaga cggctcgatt acccaatcga
     8521 ttagtttaga taaacagcta aatatcattc aagtggcgga tttggtttta ccggatgcag
     8581 aaacgacttt tgaagaatat accaccatgg aattgcttag tgtttcgatg ctaatcgtca
     8641 tttccacttt tgccttggac tttatccata gtaaccgaga aatcttcaaa attgtcgatt
     8701 tggatgaggc ttggagtttt cttcaagtgg ctcaaggaaa aacgttatcg aataagcttg
     8761 ttcgtgctgg tcgggccatg aatgctgggg tctatttcgt cactcaaaat agcgacgatt
     8821 tattagatga acgtttaaaa aataatatcg ggttgaagtt tgcttttcgc agtaccgata
     8881 ttcatgaaat caagaaaacc ttggaattct ttggtttgga tcaggaggat caaagcaatc
     8941 aaaaacgcct gcgagatttg gaaaacggga ttacgtccat ataattgtgt aaaagtgaag
     9001 catcaaaaaa gccattgatc tcagttagga gtacaatgtt ttttgcagaa aacatactcg
     9061 aataggactg atatcaatga ctcaagtaca ttttactttt gaaagcgaag aaattcaagc
     9121 aattataaac gaaagcggtg cgaatgatac cgcaaaaaca cttatgacta tcatgtttaa
     9181 tcagctgatg gaagaacaac gaaatcaata tattcaggca aatgcctatg aacgttcaga
     9241 agaacggcaa tcacaaagaa atggttacta cgatcgttcg tttacaactc gaattggtac
     9301 gttagaactt catgttccac gaaccagaga cggtaagttt agtcctacta tctttgaaag
     9361 atatcagcga agcgaaaaag ctttgatcgc tgccatgatt gaaatggtca tttctggtgt
     9421 ctccactcgc aaagtaacta aaacagttga actgctaacg gatggtgcga ctgtttctaa
     9481 atcattcgtt tcaaatctga tgaaacagtt ggatccattt gtttttgagt ggagaaaccg
     9541 gagcctagaa ggctcggaat atccattttt catgtgtgat gccctgtata tgaaagtaag
     9601 ggaaaatcat cgtattgtct caaaaggtgt atatattggt atcggcattg attctgatgg
     9661 acgacgtacg attcttggtt ttgatgttca agacggcgaa tcggaagata attgggatac
     9721 cgtttttcaa tcgttcgttc aacgaggttt gttcggtgta aaactggtca tttctgatgc
     9781 acacaaaggg ttagttaagg ctgttcgcaa gaacttttta ggtgcgagct ggcaaagatg
     9841 ccaagcccac tttttgagaa atatttttga taagctgcca aagaaagttt cttctgatgt
     9901 aaaagacgag ttgaagagta tctttaaagc ctctgaactt gaattgacac gtgaaagaaa
     9961 agagcacttc ttggaaaaat atggttgtga ttcaaaatta agtgctgctt gcgacatttt
    10021 agaaaacggc tttgaagatg ctattcaaat tttgtctttt cctgaaaata tccgccgaag
    10081 gattcgtaca accaatgtgt tggaacggtt gaacgaagag attcgccgga gagaacgagt
    10141 gattcggatt tttccaaata tcaattccat cactcggata attggaacac ttttgatgga
    10201 aaaagacact gagtggctgg cttctccacg aaaatattta gaatttaatt cgaataacat
    10261 ttagcaccat gaacctgtgc gagtctcttc tttctgagga gagacatcaa gagtcgctgc
    10321 gctactgctc ctgacctctc tcctcagaaa gaaaggcatg gcagttaggt tcacaacgca
    10381 aaacattgtg cttttgatga ggttgaactt ttac
//
LOCUS       DACDIG010000087         9964 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.087, whole genome shotgun sequence.
ACCESSION   DACDIG010000087 DACDIG010000000
VERSION     DACDIG010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9964
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.087"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            8..81
                     /locus_tag="IV247_002423"
     tRNA            8..81
                     /locus_tag="IV247_002423"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42..44,aa:Pro,seq:tgg)
     gene            101..173
                     /locus_tag="IV247_002424"
     tRNA            101..173
                     /locus_tag="IV247_002424"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:134..136,aa:Ala,seq:tgc)
     gene            201..274
                     /locus_tag="IV247_002425"
     tRNA            201..274
                     /locus_tag="IV247_002425"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:235..237,aa:Met,seq:cat)
     gene            288..361
                     /locus_tag="IV247_002426"
     tRNA            288..361
                     /locus_tag="IV247_002426"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:322..324,aa:Ile,seq:cat)
     gene            385..474
                     /locus_tag="IV247_002427"
     tRNA            385..474
                     /locus_tag="IV247_002427"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:421..423,aa:Ser,seq:tga)
     gene            487..560
                     /locus_tag="IV247_002428"
     tRNA            487..560
                     /locus_tag="IV247_002428"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:521..523,aa:Met,seq:cat)
     gene            579..654
                     /locus_tag="IV247_002429"
     tRNA            579..654
                     /locus_tag="IV247_002429"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:612..614,aa:Asp,seq:gtc)
     gene            656..726
                     /locus_tag="IV247_002430"
     tRNA            656..726
                     /locus_tag="IV247_002430"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:688..690,aa:Gly,seq:tcc)
     gene            740..813
                     /locus_tag="IV247_002431"
     tRNA            740..813
                     /locus_tag="IV247_002431"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:774..776,aa:Ile,seq:gat)
     gene            838..925
                     /locus_tag="IV247_002432"
     tRNA            838..925
                     /locus_tag="IV247_002432"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:872..874,aa:Ser,seq:gct)
     gene            947..1018
                     /locus_tag="IV247_002433"
     tRNA            947..1018
                     /locus_tag="IV247_002433"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:980..982,aa:Glu,seq:ttc)
     gene            1041..1130
                     /locus_tag="IV247_002434"
     tRNA            1041..1130
                     /locus_tag="IV247_002434"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1077..1079,aa:Ser,seq:tga)
     gene            1143..1216
                     /locus_tag="IV247_002435"
     tRNA            1143..1216
                     /locus_tag="IV247_002435"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1177..1179,aa:Met,seq:cat)
     gene            1235..1307
                     /locus_tag="IV247_002436"
     tRNA            1235..1307
                     /locus_tag="IV247_002436"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1268..1270,aa:Asp,seq:gtc)
     gene            1311..1383
                     /locus_tag="IV247_002437"
     tRNA            1311..1383
                     /locus_tag="IV247_002437"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1344..1346,aa:Phe,seq:gaa)
     gene            1388..1458
                     /locus_tag="IV247_002438"
     tRNA            1388..1458
                     /locus_tag="IV247_002438"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1420..1422,aa:Gly,seq:tcc)
     gene            1471..1544
                     /locus_tag="IV247_002439"
     tRNA            1471..1544
                     /locus_tag="IV247_002439"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1505..1507,aa:Ile,seq:gat)
     gene            1556..1627
                     /locus_tag="IV247_002440"
     tRNA            1556..1627
                     /locus_tag="IV247_002440"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1589..1591,aa:Glu,seq:ttc)
     gene            1893..3086
                     /locus_tag="IV247_002441"
     CDS             1893..3086
                     /locus_tag="IV247_002441"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAP8778471.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3216..4688
                     /locus_tag="IV247_002442"
     CDS             3216..4688
                     /locus_tag="IV247_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8778472.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            4771..5559
                     /locus_tag="IV247_002443"
     CDS             4771..5559
                     /locus_tag="IV247_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAP8778473.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(5626..6294)
                     /locus_tag="IV247_002444"
     CDS             complement(5626..6294)
                     /locus_tag="IV247_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP8778474.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            6409..8121
                     /locus_tag="IV247_002445"
     CDS             6409..8121
                     /locus_tag="IV247_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8778475.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGSPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8124..9896
                     /locus_tag="IV247_002446"
     CDS             8124..9896
                     /locus_tag="IV247_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8778476.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2899 a         1781 c         2279 g         3005 t
ORIGIN      
        1 aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa ggggtcgcag
       61 gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag ctcagctggg
      121 agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct ccatttcatt
      181 taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc ggttcatacc
      241 cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga cctttagctc
      301 agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc tacaaggtcc
      361 attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag ggaacggtct
      421 tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc tcctttttca
      481 tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga aggtcgtagg
      541 ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg taggggttaa
      601 catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc gccatgcggg
      661 tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg attctcgtca
      721 cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg cctgataagc
      781 gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat tggttttggg
      841 gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg aaaccggcgc
      901 gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc cgttggtcaa
      961 gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt acgggtcatt
     1021 aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa cggtcttgaa
     1081 aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct ttttcatatt
     1141 atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt cgtaggttca
     1201 aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg ggttaacatg
     1261 cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt ggctcggtag
     1321 ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt ctgtcccgcg
     1381 ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt gtcgcgagtt
     1441 cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt tagagcgcac
     1501 gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt ttattggccc
     1561 gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc gaatcccgta
     1621 cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga atgattttaa
     1681 aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga cagttctaat
     1741 gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag aaaactgttt
     1801 tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg gagatgcctt
     1861 gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta tttacatcag
     1921 aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat gcaatcttgg
     1981 atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct gtaacaactg
     2041 gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt caaaaagtcg
     2101 tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt gatggagata
     2161 cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa ggagttgatg
     2221 aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt ggtgctggtg
     2281 accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg cctttaccaa
     2341 tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca aatgaattaa
     2401 cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat caaggacaac
     2461 cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta gataatgaaa
     2521 caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct gaattattag
     2581 atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt ggtcctcaag
     2641 gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt tatgctcgtc
     2701 atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct gccagctatg
     2761 ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa gcagaagtac
     2821 agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat actttcggta
     2881 ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc gatcttcggc
     2941 cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa acagctgctt
     3001 acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac aaagtagatg
     3061 cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc caagtggtgg
     3121 ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt atttatctat
     3181 ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca aatgttactt
     3241 tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt acaatcgtaa
     3301 caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg aactgggtat
     3361 tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa cttgcggata
     3421 aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc ggttcgttat
     3481 tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt caagggatgg
     3541 gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat tctattgaaa
     3601 agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt gtttttggac
     3661 cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt tttatcaatg
     3721 tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa ccaaaaatca
     3781 tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc atgcttgtct
     3841 cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt gtttctgctt
     3901 tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa agagctaaag
     3961 atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc aacttggctg
     4021 cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg tggatgcaag
     4081 gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta tcactagtct
     4141 ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact cattgggtct
     4201 tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt atgcctttca
     4261 gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc ttaggtgtga
     4321 ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag cttggtgtag
     4381 caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt tctatttttg
     4441 gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta acaaatgatg
     4501 cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat gcggatattt
     4561 tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta gttggcttgt
     4621 tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt ggtaaaataa
     4681 aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa agttattcaa
     4741 aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa tatttattcg
     4801 aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac aaaaaaggtg
     4861 attggcacca gttcagagca agagacaacg gatatacctg ttttatattt acttcatgga
     4921 atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta tgtagcaaac
     4981 tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga tacagtttat
     5041 gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca tgaattattc
     5101 ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat gggcggatat
     5161 ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat ctctctttca
     5221 ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa agcatctttt
     5281 tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga tccgcaatac
     5341 ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat ggcttgtggg
     5401 gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca ggaacaaggc
     5461 ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg ggaccaatgg
     5521 attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct taaaaagata
     5581 gctcctatag agatcacaaa catagtgatc tctataggag cttttttact gctcgttttc
     5641 tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg ttagaaagag
     5701 tgctttttca ttcaattctg catgggactg catactttct ttaaatccct ctaattgatt
     5761 ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat ttccgtccat
     5821 aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa tcaataaatt
     5881 gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac gaacaagtaa
     5941 ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag tattgatttc
     6001 taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca tatactgcag
     6061 caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt ccattgtttc
     6121 agccattttt tgcaagagct gttgtttgtt tttgaaatac caatataaag caggtgcttg
     6181 aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt gtgtttcagc
     6241 caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt ccattttatc
     6301 aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt ctaattttac
     6361 agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat gaaattaatc
     6421 ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc cgtagcagtc
     6481 atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga agctattatt
     6541 acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct ggctttgcta
     6601 ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca aggcgtaagt
     6661 gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg aaatatcgaa
     6721 cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca aatccaaaat
     6781 ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat tggtgccttc
     6841 attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct tatcgttacg
     6901 gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat gatcattcaa
     6961 aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat tcgtgtagtg
     7021 aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag tgaggactta
     7081 acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc cttcatgctg
     7141 gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt taaagatgat
     7201 cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat tatgatggca
     7261 atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc gctgaagcgg
     7321 attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc agaacaagag
     7381 ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga ggatacagat
     7441 acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat cgtcggggcg
     7501 actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga tccgacagaa
     7561 ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag tttaagacaa
     7621 acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc tcaaaacttg
     7681 cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc tattgctcaa
     7741 gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga agaacgtagc
     7801 agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt tatcggtagt
     7861 ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc tgaaaagctg
     7921 gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat cgcacaaaaa
     7981 attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag attagtcggc
     8041 gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat ctttgaaact
     8101 caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa tttttctatc
     8161 actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg atcattgcta
     8221 cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa ttagctaatt
     8281 acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct gtaatatgga
     8341 aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt attttgttca
     8401 cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac aaattggaaa
     8461 aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt cgttttacca
     8521 gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc atcacgaatg
     8581 ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag ttagcttggg
     8641 ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc cgcaaagcac
     8701 ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat atggatgaaa
     8761 aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg attgaaggat
     8821 tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct tattctggtc
     8881 tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt atctttttcg
     8941 gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc ttagttgtga
     9001 cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca tctggataca
     9061 gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt gatgaaaaag
     9121 atgaagtcaa accagaaaac ggtaaagcat ttgatggtgt tgaaaaaggt cttgcgttgc
     9181 agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc tcgatcaatg
     9241 tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa acgacgatca
     9301 tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc gatggaatag
     9361 atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt ttgcaagaat
     9421 ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg gacgcttcag
     9481 atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc atgacgttag
     9541 aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca ggtcaaaagc
     9601 agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt ttggatgaag
     9661 caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg gatgaagcaa
     9721 tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg aatgcagata
     9781 gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcat gagttattag
     9841 cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa agttaaatta
     9901 aaagaacctc gttataacgg ggttcttttt atatatttta tgttgtttgt ttttataagg
     9961 ggca
//
LOCUS       DACDIG010000088         9686 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.088, whole genome shotgun sequence.
ACCESSION   DACDIG010000088 DACDIG010000000
VERSION     DACDIG010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9686
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.088"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            5..610
                     /locus_tag="IV247_002447"
     CDS             5..610
                     /locus_tag="IV247_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP8778477.1"
                     /translation="MAKTTTVTTFDNISIKRISFNFKNATNAISTDCNGQLDGETEMQ
                     RIVKKCGSTEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDERIKSGIYSYGPDSVG
                     EDFSLAAEIVDDFEEKNKLIGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFGK
                     FYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            630..992
                     /locus_tag="IV247_002448"
     CDS             630..992
                     /locus_tag="IV247_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778478.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            995..1177
                     /locus_tag="IV247_002449"
     CDS             995..1177
                     /locus_tag="IV247_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778479.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            1194..4613
                     /locus_tag="IV247_002450"
     CDS             1194..4613
                     /locus_tag="IV247_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP8778480.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITVIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWVSIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSIFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGEAGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            4664..5401
                     /locus_tag="IV247_002451"
     CDS             4664..5401
                     /locus_tag="IV247_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP8778481.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVVGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINYFNCTGLSKIQIYRNGLR"
     gene            5411..7702
                     /locus_tag="IV247_002452"
     CDS             5411..7702
                     /locus_tag="IV247_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778482.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITASQAWQNAKTLAKPVIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDNGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            7726..9408
                     /locus_tag="IV247_002453"
                     /pseudo
     CDS             7726..9408
                     /locus_tag="IV247_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737855.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3392 a         1491 c         2064 g         2739 t
ORIGIN      
        1 aacaatggct aaaacaacaa cagtaacaac ttttgacaat ataagtatta aaagaatttc
       61 ttttaatttt aagaatgcaa caaatgcaat ttcaactgat tgtaatggac aattagatgg
      121 cgaaacagaa atgcagagaa ttgtaaaaaa atgcggttca acagaagtaa aatcgaaatc
      181 taaaccaatc aatatgacgg taacaattac tgcacatgta ccgatggaag tttatcgacg
      241 cttcaatggg ttgaaacaag atgaacgtat taaatcggga atttactctt atggtcctga
      301 ttccgtaggg gaagatttct ctcttgctgc agagatcgtg gatgatttcg aagaaaaaaa
      361 taagctgatt ggtatgttag catgcacttc gaatacagga ttaacattct ctattgaaaa
      421 tggtgcggat gaagtagctg cgttagaact agaaacaaaa gttatgcaag atgaatttgg
      481 taaattttat catgaagcaa ttgttgcaga acttgaagaa gacttaacag atcaatggat
      541 gacgaatcta tctgctgatg tgattaaaaa aacttcagtt gtgacaacta cggccactca
      601 atcacagtaa aaaaaacgga ggtagcgaaa tgaacgaaga ttactcaaaa attgaactaa
      661 acgatggaac aattttgaat ttagaaccta aactgaatat caagaaatta ttgatgatca
      721 atagagattt taacacagac gagtttgcaa aaatgactgt gggaaaagga tccatggata
      781 tttctgttat tcaaggtgca aaggctgtgt atattgctta ccgccaagcg aacatgactg
      841 attatatttc attcgatgaa tttatcgata aatgggattt tgatatggct actgccagct
      901 atatttatca attgatgatg ttcaaacaag cacgcgatgc ttatcaaaaa gaatttgaaa
      961 aagcaaataa ggaaaaaaag cttcaaaagt aaaaatgcca aagctcttag ttgaaacgtg
     1021 ggtcgatgtc tattcgatgt tgaccgacgt tttttctatg ccttcagatt tggttttaag
     1081 cgatatctgt ttagatgaca ttttgcaaat ggcttacaac aagagtgctt atgaaggatg
     1141 gaaaaactat gcaataaacc aatcccagaa aaactaaaga aaggaggtaa aaaatggcta
     1201 aaaagagaac agaagcagaa gtaacattca tagctaacga tgacggattg aaatctacgt
     1261 taaaagaaat cagtgctgaa ttaactaaaa atagagcaga attaaaacta gaacaagctc
     1321 aattacaaca gactggttct gaatcagaca agttaggaag taaattatct tctttagaaa
     1381 agcagtatga attacaaagt caaaaagttg aagtaactag ccaacgttta gccaatgcca
     1441 aaaaatatta tggagaaaat tccaccgaag ttcagaaact tgagagagaa ctgattaacc
     1501 aacaaacagc gcaacaacgt ttgtcaaacg aaattgataa aacgagtaat gcactagctc
     1561 aagcaaaagg cgaaatacag acgtacgagt ctacaatgca acagttggat agtgaacaaa
     1621 aaaatgttca agctagtgct tctctgattg aatccgaata caaaaaatgg caagcaactg
     1681 ctggtcaatc agcttctgaa tccgagaaat tagcgaaagc ccaagaatat gtttctcaac
     1741 aatctgaaaa tgcagagaaa acgatagata tcctgagacg acagttagaa gctacacagt
     1801 ctgagtttgg cgctacatcc acagaagcaa tgcagatgga ggcgaagctt aatgatgctg
     1861 aacgtgaatt tgaagagtta ggacaagctg ctaaaaatgt agatacaact aacttggacg
     1921 atatcggaag caaaatagat atgaataatc taatggaagc ttctgacgtt ttaagcgaca
     1981 ttggcgataa gcttacagaa ttagggaaac aagcagtgga ctctgctaat agtgtaggta
     2041 gttcccagag taaaatacaa gctaattttg gtttgactaa acaagaggct gaagaattaa
     2101 cgaatgtagc cagagacatt tattataaag gttttggaga atcgttagat cagtccacag
     2161 atgcattgat tttggtaaag cgtaatttag gcgatttaaa taatcaagat ttacaaaata
     2221 tcacggaaca agctatggtc ctagaaaaca ccatgggcgc tgatatggat gaaacgttac
     2281 gtggtgtaaa tggcttaatg gtcaatttcg gcttgagcgc tcaagatgca atggatttaa
     2341 tggtttcggg tactcaaaac ggtttagata aaacgcacga attaggcgac aatatggcag
     2401 aatatagcca attatggagt caaatgggat attcagctga tgaaacgttc ggaatgcttc
     2461 aaaatggttt agatgcgggt gcttataacc ttgataaagt caatgactta gttaaggaaa
     2521 tgggaatatc gttaacagat ggtcgatttg agcaaaacat ggatatgttt agtgaaagta
     2581 ctagaaaagc ttttgaagag tggaaaaatg gcggaggaac acaaaaagac gttattaatt
     2641 ccatgattca agattttagc aatatggatg gtcaatacga ccaattaaat aaagcttcta
     2701 caatttggtc tgcacttggc gaagataacg cgatgaaagt tgtccaatct ttgactgatg
     2761 ttaaccatac atttgatgat gttagtggat ctgcacaaaa aatgaatgaa gattctacta
     2821 ctccgttaca agaattaaac ggaaaaatag ctgaattaaa ggattcatta gctcctatag
     2881 gcaacacaat catagatgca ctcgaaccag taattgattt tctaggaaag atggctgatg
     2941 cgtttaataa tcttccacaa ccagtacagg attatgccgt agcgattggc ggattgactg
     3001 ctgcatttac tttattaatg ccaataatag ttggcttcat ggctctaggt ggtcctacta
     3061 cattaataat aggagcagtt attactgtta ttgctggagt tatagcaatt ataaaaaact
     3121 ggggcgcaat tactgactgg tttaagggaa tatggagtaa attcactgat tggttgggtg
     3181 gtacttggga aagtataaaa gaaggtgcct catcagtttg ggatggagtt aaagaaacct
     3241 ggtctggatt tgtagattgg gttcaagata tttggcaagg agtttctgat tggtttggag
     3301 agctatggag cggattagtt gaaggagctt ccaacatctg gcaaggagtc caagagactt
     3361 ggcaagcatt cgttgattgg gtttcaaata tttggaacgg agtcaaagaa gtatggtcga
     3421 ttatttgggc agacattgta ggaattgttc aaataccatg gaccttaata acgtcattga
     3481 ttcaagccgg tattaatatt atcgtgggta tttttgatgt agctggacag ttattaggcg
     3541 cagcttggca agctgtttgg acacctattt ctgatttcct taaaaacact tgggatacta
     3601 tgacacaatg ggtaagcatc gcttggaatg gaattgtaac tacattccat actatatttg
     3661 atccagtagt ggcatggtgg aatggtatat ggacagctat tagtactacg gcttcaaata
     3721 tttggaattc aattagtgca acagcttcta gtatttggaa cagtatcaag aatacaatca
     3781 ctagcttggt acaagcagct gctacagtaa ttcaaaatat ttggtcaact gtatctagtt
     3841 ggttaggtgg aatttggaat tcaatcagct ctacagcatc aaatatctgg aatagcgtga
     3901 ctagtagtat aagcaatgct ataaacgcag ctaaaagtgc cattcaaagt gtttggaata
     3961 gtatatcttc gtggatcagc ggaatttgga acggtatcaa aaacactgct ttgaatcttt
     4021 ggaatggaat tacaagcact attagctcta aagtaaacga tggaaaaaat gcaatttcaa
     4081 gcggttggtc caatctaaca ggtattgttt ccgacatatt caataatgtt aaaagtacaa
     4141 ttgctaacat ttgggaaggt atcaaaaaga ctgttagcgc tccaattgat tggatcagag
     4201 ataaaatcag tagtatcttt gataatttga atatttctat accacatatt ccgttaccac
     4261 attttaaatt gagcggagaa ttcaatccat tgaaggggaa aatcccaacg ttgggtgttg
     4321 attggtatgc gaaaggtagt gtgtttaatt ctccgaatat tatcggtgtc ggcgaagcag
     4381 gacctgaagc agttttacct ttgaaaagat ctgtgctgca agaaattggt gatcgtatct
     4441 tgagtagcac atcagtttca tctagggcac aaacgattca acctgtgaat aactacgaat
     4501 tcaatttcac aattgatggt aacgcagatg aggttactat gaagcaaaca actcaacaaa
     4561 tcattgatag cattacaaaa gttcaaaatg ataatgcttc ggcatggcgt taaacaggag
     4621 agtatttctc ctgttttttt agtattaaaa aggatgtgaa aaaatgactg attgtataca
     4681 ttctataatc gatggatttc ctgattattt gcataaattg gctttagctg aaagaccaac
     4741 cataccttct ccaaaaagac agagagttga aacttctgtt ttaggaaggt taggtggctt
     4801 agtacaagat tactcgtttg aagacatgtc gtttacattg cactataact atttagagga
     4861 tgtggaagac catcaagcgt tcaagcaatc gttttatatc atgcgtcatt ggttaaatta
     4921 tgcaaagaaa ttagaattct ctgatgatcc caacgtctat tatgttatcc agactatcga
     4981 tattggggat gcagaaaacg atattgttga atggggagag ttcgatgtaa atattactgc
     5041 gaaaccattc gcaagagttc aagaagatgt acctataacc gtagataaac cacagtcatt
     5101 taacttgctg aataatagtt tagaagaaag ttttccaaag attatcatca ctccttcagc
     5161 tacttcatgc cagttcatct taaatgatta tgtgtttagt tttgaaggct tagtagtagg
     5221 aactgacgta gtcattgata gtgatttgat gctttgctac gaagagcaat cggacggaga
     5281 tattttagat cggtccaaca aaatgaagac catgcaatat ccgacattgc aagtggatat
     5341 taattatttt aattgtactg gtttgagcaa aatacaaatt tatcgtaatg ggttaaggta
     5401 ggtgaaatag atgatcgata atttaataac tatttacgat aaaaacgacg cgaataattt
     5461 agctgaacat ttatatgata cgcaaggttt aggcgctttg tcagactggt taacagctac
     5521 tgttagcaat aaattaaacg gagccgagat atttcagggt acttatccaa taagcggaac
     5581 taatgcagat ttgattgtag aaggacgtat tattcagtgt tatgtagatg aaaatcgagc
     5641 aaaacagcgt ctacggattt attatgcaaa gacttctgta ataggcaata cgatagaagt
     5701 aaaagctgaa cctattttca atgatataag aaaatcggtg ttgaataaat atgacagtgg
     5761 aacagaaaag atcactgcta gtcaggcatg gcaaaacgca aaaactttag cgaaaccagt
     5821 tattccttca cagttttctt tctcgtcatt agtagatacg cttgctaatg tgaagataga
     5881 aaaggcgaat tttttagaat tctttggtgg aaaagaggga tctattctag atcgatttca
     5941 tggggaattt ctaaaagata ataacacatt acgtcatgaa aaaaggctag gcacggatca
     6001 taaaatcaaa gcgatttata ctaaaaactt aactggtctt gacttagaga ttgatgctca
     6061 aagtgtttta gttggagttt atccattcat tagcagctct tcagaaggag aagacgagat
     6121 cactctacca gaagaagtta ttttcacgga ttacgtggat gattatcctg ctggatatgt
     6181 ttcttttgtt gattttaaag acaaagcgac tgatgtagcc acattaaggg aagctgctaa
     6241 agactggttg aaaacaaaca tagataaaca aaaaccacaa gtgagtggtt cgattgaatt
     6301 agtaccattg aggcatcaaa gaggctatga aaaatttgtt gatctagaaa aagtttcgat
     6361 gggtgacgga gtagatgtgt atcatccaca gttaaaagtg aatatgtcag cgagaattgt
     6421 ggaatatacg tttaatgttc taaccaattc atacgataaa ttagttgtag gaaacgtcaa
     6481 aacaaacttc ttagaaaaca cagagaataa tgtcagcaat ttgattaatg atgccattga
     6541 tcaattgaaa aacggtggcg aaatcagtga tttactcaat gatattgtag atcatcaaac
     6601 tgatatgatt actggtcaaa atggtggtta tgttttatta gatcctaaag aagcgcctag
     6661 tcgtattttg attatggaca caccagataa gaataccgca aggaatgttt tacaaatcaa
     6721 caatgctggt attggttttt ctaaaactgg cattaatgga acatatgaaa cggcatggac
     6781 gttagatggc ggattcaatg cctcgtttat tacggctggt gaaatagtag gaattactat
     6841 tagaggtact acattaatta gtgatggtgc tgattataga acaagtattg ctaatggcaa
     6901 aatgacttgg tactcaaaaa aagttaacaa agatattatg gagctagaag cacgtgatta
     6961 tgtaagtgct gatgccggta ttgtatcata caccatgaaa actggtggtg gtttcatgat
     7021 tagaaatcca cagggtaact tggtttttag tacgtgggat aatggtaata acagaccgtt
     7081 tctatctttt ggtgcgccca atttcaggta tagcaatgct agttatgtaa cttctggcga
     7141 cggtagttct ttaagcatta atggtagtgc gggtaactca tgggaattta aggtagctgg
     7201 taggactatg aaatttacta gtgatggtat gctaacgtta ccaggttgtt tttttggttc
     7261 atgggaagat gggaaacttg ctaggtttga acaatcaacg gtacaagtat ataaagattt
     7321 tactgttaga ggtactaaaa actcaactgt accaacagaa cattatggac aacgactatt
     7381 gaacgcttat gaaactccag aatattattt cgctgattat ggggaagccg ttacaggtga
     7441 caatggtaaa gttcgtgttg atattgaccc catgtttgct gaaacagtaa atctaagtcg
     7501 gtatatgaca catgtgacac ctacagaact agttttgtgt gctgttactc atgaagatat
     7561 tgaccatttc atcattgaaa ctagtaagcc aaacgtatta gttagatgga atttagtggc
     7621 acaccgtcta gggtatgaag atattagatt aaaagaggat acagcatatg atagcacagt
     7681 gcttgaccaa aaacgttttt aaaacgaaga caaggaggta tataaatggc tagcagttta
     7741 tataatttgg ctttagattt cagcaaagaa ttaaactaca ccaaagctat tatggctcgt
     7801 caaggtgata aagggattac ggtgacggtt aaaccgtttc taaatggctt gcagatggat
     7861 acgagtggcg gaacatttac tttaaaagga acaacaccat ctaaccgtta cgtagataat
     7921 gttgcaacta gtgtaactag tgaagaagtc acgttttctc ttgatggcac atttatgagt
     7981 gaagcaggat attataaaca ctgctacgta gaatatagaa aagacaatca aattttaaca
     8041 acgcaagata tcattttttt ctcactagga gtgtctgaca tttcgcaagg ccaagccgat
     8101 gaatatgttt cgcaattaga agagttgatt cgaaagtaca acgaaacttt tgatgctttt
     8161 atggctgaaa tcaaaggtag agtggatagc ttaaatcaac agattactga tttaactggt
     8221 caagctaaaa cgctacaaga caagttagat gctctgaaag aagaaatttc taagttaggt
     8281 aacttacaag tgatgtacag taacagcatc gacttcgggg gctacgatta tagcgggaat
     8341 ccgaatttaa tggctaatat aaacgctgac agcttctcgc aaggtagtgg tgccttatct
     8401 gttgtagatg atggtgacga ggtagtagtc acgcttgacc caaacaataa attagagata
     8461 ttcaaagcga aaagtcaacc agctctggta gtaggaaaaa catataccat gagcgtagaa
     8521 attatgctag aaggcgattt cactggagac cctagcaaga taggcctaag atatattaaa
     8581 atgcctaact gggtatcagg gctatatacg cgtaatacat taactgctac taagggtgta
     8641 tggcaaaaac taactggcac cgttaaaatt actgctgcga gtgataacgc tgaaagctgg
     8701 tttataatgc tacaaaataa agacgctaat aacagcctat ccggcaaact acgtttgaga
     8761 cacgctaaac tcgaagaagg ctcaacagcc acaccatatc aaccaaattt actcgatgcg
     8821 ccgtattatt tgagtaaggt ggctttgggt gagaatattg ctgatcctgc agttagcttc
     8881 ccaattaaaa cgagcgcata ccgtttatac ggtgtgaaca tgctagaaga gtttaaagtt
     8941 ggtcaaagat atacaattac aataaaagga actaaaccag caactcaaga tttttgggct
     9001 tacaacggtg ggaatatttc attagagaga atgactccag tagaagggtt agttgacgtg
     9061 tggacttgtt catttactat attaaaacta gatagtagtt caccaagtct attgagtata
     9121 tatcaaacac cacaatcaac agtaggctca tgtcaaattg actggctcaa gatcgaaaaa
     9181 ggcgacacac gaaccccgaa tattgagcaa tataaatacc gaggaatcgg catgcgagac
     9241 tcaaacaatc caaaagatta tgtttgggat ctagcaccag aatatgtcga agataatttg
     9301 gccacagata ttaaaatttc tgaaattact ggtaaagcaa acaattatac cgatgggaaa
     9361 gtatcggaga ttaattcgca gttgactgct tcaattaatg aagtagacac cacagctaag
     9421 gatgctcaaa caaaagcgaa tgctaatgcg actgctatag atgaattaga caataagatc
     9481 gatgaacgca ttaatgatac agctactacc acattaacag ttacaaacgg gaataccgga
     9541 tcagcaaagc tttatcgtga aggaaaaaca gtttctatat attttgtggc tttaaacgga
     9601 aaaagcagtg gtggaaatga ttcaacgata ctaacaattc cagaaggcta tcggccacca
     9661 attagttttg agcaactggt tggctc
//
LOCUS       DACDIG010000089         9524 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.089, whole genome shotgun sequence.
ACCESSION   DACDIG010000089 DACDIG010000000
VERSION     DACDIG010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.089"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            326..922
                     /locus_tag="IV247_002454"
     CDS             326..922
                     /locus_tag="IV247_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP8778483.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(980..1462)
                     /locus_tag="IV247_002455"
     CDS             complement(980..1462)
                     /locus_tag="IV247_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778484.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1568..1765)
                     /locus_tag="IV247_002456"
     CDS             complement(1568..1765)
                     /locus_tag="IV247_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778485.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            2060..2818
                     /locus_tag="IV247_002457"
     CDS             2060..2818
                     /locus_tag="IV247_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAP8778486.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            2878..3306
                     /locus_tag="IV247_002458"
     CDS             2878..3306
                     /locus_tag="IV247_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778487.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            3352..3424
                     /locus_tag="IV247_002459"
     tRNA            3352..3424
                     /locus_tag="IV247_002459"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3385..3387,aa:Gln,seq:ctg)
     gene            complement(3574..3945)
                     /locus_tag="IV247_002460"
     CDS             complement(3574..3945)
                     /locus_tag="IV247_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP8778488.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            4160..4345
                     /locus_tag="IV247_002461"
     CDS             4160..4345
                     /locus_tag="IV247_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778489.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(4428..4616)
                     /locus_tag="IV247_002462"
     CDS             complement(4428..4616)
                     /locus_tag="IV247_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778490.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(4640..4903)
                     /locus_tag="IV247_002463"
     CDS             complement(4640..4903)
                     /locus_tag="IV247_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778491.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            5061..5318
                     /locus_tag="IV247_002464"
     CDS             5061..5318
                     /locus_tag="IV247_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAP8778492.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(5389..5580)
                     /locus_tag="IV247_002465"
     CDS             complement(5389..5580)
                     /locus_tag="IV247_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778493.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(5721..6041)
                     /locus_tag="IV247_002466"
     CDS             complement(5721..6041)
                     /locus_tag="IV247_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778494.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(6064..6498)
                     /locus_tag="IV247_002467"
     CDS             complement(6064..6498)
                     /locus_tag="IV247_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP8778495.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            6833..7471
                     /locus_tag="IV247_002468"
     CDS             6833..7471
                     /locus_tag="IV247_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8778496.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(7795..7878)
                     /locus_tag="IV247_002469"
     tRNA            complement(7795..7878)
                     /locus_tag="IV247_002469"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7842..7844),aa:Leu,seq:cag)
     gene            8406..8906
                     /locus_tag="IV247_002470"
     CDS             8406..8906
                     /locus_tag="IV247_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8778497.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(9096..9440)
                     /locus_tag="IV247_002471"
     CDS             complement(9096..9440)
                     /locus_tag="IV247_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAP8778498.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
BASE COUNT         2987 a         1846 c         1485 g         3206 t
ORIGIN      
        1 actaaaaaac ttatttaaga aatataaact aattttctat aaaagcaagt aatttttaat
       61 ggcaatatca taaaaaaaag aagatagaga tttaataatt atatatcagc tgtttttttc
      121 aaataagcaa aaattcctaa aacacaacaa tgaaagcgat aactaagaaa atccttacat
      181 aacgattttt cttattaaaa ttgttttttg aacttcgctt attttttgtg agcatatctg
      241 tttacttact ttttgtaagg tgctatactt catttcgtaa tcaattaaaa aaatgcaaaa
      301 cgcaaatcaa aggagaaatt ttattatgac aacttttact gacactttga aaaaccgccg
      361 ttcaatctat catctaggac gtaatgtttc actttctaat gaagaattaa caactttgat
      421 caaagaagca attaaagaaa gcccaactgc tttcaatgct caatcaacac gcgcagttat
      481 ccttttcgga gatgcacatg aaaaactttg ggaaatcact gaagaagcac ttcgcccatt
      541 gactccagca gaagctttcc caaatacaca aaacaaatta gctggtttca aaaatggtta
      601 tggtacagta ttattcttca aagatactga cgtggtaaaa ggtctacaag aacaatttga
      661 attatatgca gacaacttcc cagattggtc agaacaatca aacggaatcg caacagctaa
      721 tacttgggta gcattagttg ataaaggatt gggcgcaaac ttgcaacatt acaacccagt
      781 tattgacgaa gcagttgcaa aagaatggaa tatcccttca aactggaaac tacgttcgca
      841 attagtattc ggttcaccag aaacaccggc tggggaaaaa gaatacatga acgatgccga
      901 tcgcttccgc gtatttggat aattttattt gaaataaaaa acagaggttg tgacaaacat
      961 cttgtcgcaa cctctgtttt tattctggat caatcataat atcaatctct gcctcatcgt
     1021 ttaagcgttg ggacaaaaat ggaactaatt cttctaatga tgcagaaaat ttcagcatcg
     1081 gtcctcgcca tccgtttcgg aaaaaattca tttttacgat tttagaacct tttggctgat
     1141 ataatacaat tttattgatc tcagaaaaag gaacaccttt tttgtcaaag ctattcaaaa
     1201 caagtccttc ttctgtgatt cctctactgt ccaacaaaaa actaataaaa atcattgctg
     1261 acaagatccc ttttgccaat tcatctgctg taggatgact gatgagtgta taccaaatga
     1321 ctagtgacac taagaagggt atcaccaagt agacccatcg atttcgcaca gtccgataaa
     1381 taatttcttt tcgaaccagc caaagaaata aaaacatcac aacaacgaat aaaaacaata
     1441 gaaccgtaaa gaatgttacc atgacaactc gcctcgcttt ttcttctcca gaaaatagga
     1501 aaagaatact gatttacctt ctcaaagaaa ttggacttgc agcacgccgc acgtccaatt
     1561 gttttcttta ttcaaatgta tcatattttg caacaaccat gcttgctttt tcatctaata
     1621 attcttggtc ctctgcagca cattgggcaa ctttgaccac ggcgcagttt gtcatttttg
     1681 atacaacttc accaatgacc gtttttgtaa aaccaattgg tttgcatgca tattgttctc
     1741 ctaaattaat catttcttcc gtcatcaaaa tcctcctaaa ctgctggaac ttttctccac
     1801 attaccactg ctatttcttt ggagatttaa ctcattgatt tttatttaca gttaaaaatc
     1861 aaccttacag gcattatagc gtactgtttt cgttttcaca attaaaacag ctgttgtatt
     1921 tagaagaatt tttttcaaaa aattaaaaca tattccttaa aaactaatta tttcctacat
     1981 attaaaatat aatacttcca tttccaataa aaatacattt ataataggaa agataaacag
     2041 ctgaaaaggg agatttcaaa tgaagaaaaa aacgttcaaa aaactattgt ttctaggggg
     2101 cacagctgct cttttattaa gcggtacaag ttattaccta catatccatt ctccagaaca
     2161 tacaatgaac actgcacaaa aaagttcaac acaaaaaacg aaaaagacaa aagagaaagc
     2221 agaagtttta ctagatgttc cattagagag ccagtttgat tctccttctt tagaaaatgg
     2281 ctgtgaagtc acttcgcttt ctatgctttt atctttttat ggttatgaaa caactaaaaa
     2341 ccagttagct gagcaattgg attacgtccc tgttttcaat gcagatggga ctcatggcga
     2401 tcctaatgaa ggatttgttg gtgatatcag tggcggagac tgggctatgg tcgtctatgt
     2461 tcccccagtt gcctcccttg ctcagcaaat cgtccaaacg gattatcaca cttttccaaa
     2521 aaccgatgct caactcgatg atatcaaaaa agcactatct aaaggaaatc ctgtctggac
     2581 ttctgtgact atcaattttg aagtcccaga tgaaactgat tttatgactt ggaccacaaa
     2641 taatggagaa gtaagagtca ctcctcttgt tcatgcttgt gtagtcactg gatatgacaa
     2701 agaaaatatt tatgtcaacg atccctatgg tgtaaaaaat cgagcagtac cgatcaacga
     2761 tttttcggcg attttcactg caatgggtgg acagatgcta actttggaga aaagctagta
     2821 attcaaacaa aaatccctta tactcaagat accaaacctt tgaagggagc gtagataatg
     2881 gaaaaattca cacataaaaa aatggatcca aatgaaattc cgattatttt tgtccgtgac
     2941 tgcaagggaa atgttcaagg taaagtctct attaatgagt ggaacgagcg tcgcagacct
     3001 gctaccctca atgaactgga aatcaaattg tatcgtcaat ctcttgttta ttacgcagat
     3061 caagagtacg agaaagccac cgatttattg aaatttctaa ttgctcgaac agagtatact
     3121 cgctttgaat atattgaacg tttagcaaac atctatcata ttatgaatga acctgtcaaa
     3181 gaatatcagt tattggacac cgtcttgtca gtagcagaac tgattgccct tcctgctgga
     3241 ttagaaaaga aactagtacg ccgcctttta cgagtaaagc aacaactatc cgatcaagaa
     3301 aaataacttg cgttttttta ggaaagtgct atgattattt tgttgaataa atgggatgta
     3361 gccaaactgg taaggcactt gactctgaat caagcaattg taggttcgaa ccctgccatc
     3421 ccaatttgat gtgcttgctg aaaaaatgaa ccccctgtaa aagaacaatc tttttgcagg
     3481 gggattattg tattattttt actacttgta tttagtttac ataattaaat tatgaataat
     3541 aaaatcgggc gtattcttaa tacaaaaact gatttaccca ataattgcta atgctttctc
     3601 cggaactttt tctctaggaa cttcctcata cgtttctatg tgtgctcctt tattgctaat
     3661 tttcaaataa tgattttctt ttaacacaga aagaccatta aattcaaccg ttcgttcttt
     3721 cccattggca tcagcagctt tctgcacata ccttgctgta ccatattcat ttacttcctc
     3781 tggtttttgt gtctttacat agacctctcc tttttcaact aatggattta gctgatcaag
     3841 tataccaggg atttctccat aacttccttc agtataatat ttcagtccaa atagcaaaat
     3901 accactaatc gccgccagtc caatcaaata tttaatcact ttcatctttc tacctccttg
     3961 ttatatattc agtatatggg tcagttaaag aaattccatc cgtcccaagt tggagatttt
     4021 tctcctactt tagaagcgag aatcgagtgt ttttttagta aaaatgatcg ttatttgata
     4081 ttattgacgt gtggataaat cagtttttct cgttttgtcc atgtaaaaaa tcatgttgat
     4141 agcaaaggat gtgttcaaaa tgaatcaaga agaattattt caaaaagtca aggaaatgat
     4201 taagaacggc aatttcgatg gcgcaaaacg atttatcgaa gaacataaag aacaattagg
     4261 accttataaa gagaaagccc aaaatctttt aaaagatgta aatatcgaca gtgtcaaaaa
     4321 caaatttaaa aatctattca aataagtaaa actttcactt ttgtaaaatc aaaggaggac
     4381 aatgctgctg catcgtcctc ctttggttct tttctgaata cttcatacta aaataaacgt
     4441 gtcggatcgt aatgataagt cacattgccg ttcagtgagg cttttcccac cacatattta
     4501 cgattctcca acttcagcca cgcttctcga aaagccatta attcttctct ttttacatca
     4561 atcttttcga tttttccatc gatcaaatct tgaataagtt gttcatatgg attcattttt
     4621 ttcctccatc tgctgtcaat caataagttg taaaaatagg ttcatatgct tttttggtat
     4681 ctagatcttc tctgactttg actgtcgtat aaataatttt ttctactggg atactataat
     4741 aacttccttc accattattg aatcggtgaa cgtctttctt tgctaaaata ccatttattt
     4801 tattcgtcag tacacgttgt tcgacactgt tacttacaac atagtaaatc acccgaatat
     4861 tattgatcaa atgcaaatcg atttctaaca ctacatgatc catgaaaatc ccactccttc
     4921 tttcactttg attatagcaa gtttctcctc taatttcaaa atcgatacag attttaccgg
     4981 ataaatccac aaaactgctt gcgccatttc gcacaaagtg ttattttgta tcatggactt
     5041 tatttatcgg aggcaatagt atgatcgcga tcggtcaatt tgttttttat attccttttt
     5101 ttattatgat aagcattctt ttctattaca taaaatggac aaaaaagaag ttttcagttt
     5161 tactggcctc tttacctgct gtatatttta cttatcaaat tttttctttt aggcattggg
     5221 aaacaacgtc tgtattaata acacacatca tcgaattgac gctttcagta atttttctta
     5281 ttatttggat ctatttttta tataagaatc agaattgaat ccctcgcatg tttaaaaaga
     5341 aaacgattaa agatgcagca aatttctgcc gatctttaat cgttttttct attctgttat
     5401 accaaaaaaa tgtttgaatc ggtgaaggat cgttccttgt tttaattcta taaattcttg
     5461 tgcgatcggg tacttttctt ccaaattttc tttgttcttt tcgatcaggc ttttcgcttc
     5521 ttcatatttt ttatctgcac atagtgcttt tacttcctgt aatacttttc ctgcttccat
     5581 ttgcctcacc ctcttgccat tttattgctt taagttttat ttaagttata cgcattattc
     5641 tagtttcatc ccaaataacc ttttttcatt tttactcctc gaactaatca gttttctaaa
     5701 ggctgattgg ttcgtctttt ttatttttcc tccatttcat tctgctcttc tgttaattta
     5761 tctaatgctt tgattgatgc tgcggctctt gcataatcat agctgtataa ttccataaat
     5821 ttatcaggca gaacaaacca cgctggatga taaccgttag ctgtgacagg gaatccttta
     5881 tcttgttggt tatctccatc ccatctcaga gcaacacctt tttttccttt ccactttact
     5941 aaagcgatcg agtatccctt ctttgtttgt gtgccgtctt caataattgc taaaatttct
     6001 attttttctt ttggcgcacg gacttcttga ggacgaacca ttttctattc ctcccttatc
     6061 tcattacttc acgaccaaca cactgcaagg ggcatgtgat acgacataag aagcggtcga
     6121 tccaactaat gcttgttgta tagcaccttt tcctgtcgct cccataacga tcaaatcaat
     6181 attttcttgt ccagggatga cattggcaat caaatttctt ggatctccag tttcaactat
     6241 tgcgtggatc tcagcaatgc cgtactcttt ggcatcattg atttttttca gcatggctac
     6301 ttctgttctt tgtttttcat ctgcatagat ttttgaaaag gcataggcac tatgggtcag
     6361 ctcaactttg ttgatgattg acacgataaa taagtgggct tcattgtcct ttgctaagtc
     6421 aagggcttca gcgaacgctt tgatcgattg tcttgatccg tcaactgcta ccatgatgtt
     6481 ctggtatctt ttactcattg gaaccgcctc ctttttcttt tattgtaaca tctcaagcaa
     6541 aaatacaaaa aatatacgca atatgtaaat aaaatgatga acaaaagcaa tttctttcaa
     6601 gttatttata catttctttt ctcatcagaa cacctgttct aatcccatta tttctgcatt
     6661 atgtgaaatt cctcatcatt tttcacatcg agcgaacatc attctgttac aaaaaacgtg
     6721 gatttgtcat aaattgtcat gcttctttta cctatgaaat attttcacgt gttaaagtat
     6781 tcctcgttga acacaacaaa taaaactttt taacgaataa aggagatttt tcatgacatc
     6841 acttaaaact ttactttttg gaacaacttt ggctgccggc gctgcattct tcatgggaac
     6901 gactgctcat gcggacgaag cttataccgt tcaatcaggt gatactctat caacgatttc
     6961 tcaaaaatac gttggtgata actccttgat caatgccatt gcagaatcaa attcaatttc
     7021 tgatattaac ctgatctact caggacaaca attaacaatc ccaacagaag gctctgcaca
     7081 agcagctgct gaacctcaag cagccgttca agaagcacca gtccaagctg aacctgttca
     7141 agctgaacaa cctgttgtac aagaaacggt tcaaacggaa acacaagcag cgcctgttgc
     7201 tgaaacacaa cctgctcctg ctgtaacaga aactgctgct actccagcaa gtacaagctc
     7261 tgcaaaagaa tggatcgcac aaaaagaatc aagcggttct tacacagcaa cgaacggacg
     7321 ttacatcggc cgttaccaat tagattcttc ttacttaaat ggtgactact ctgctgcaaa
     7381 ccaagaaaga gtagcagaac aatatgttac ttcacgttat ggttcttggg aagcggcgaa
     7441 agcattctgg gaagcaaacg gttggtacta agattcatta tttgaacgtt taaacacatt
     7501 caaaatatag taagaggtca tgcgatttat tctttcgcat gacctctttt tgagtttaag
     7561 tttagtcttt attccttaac tatcgtttgc cagtatttct tgtatcagaa tctataaatc
     7621 tactatttga atcaatctat taggttttaa actgtcccac cttcacacaa attttttttg
     7681 atttatttga atctggttcc gttgtttaaa atttttccta tattctttta gataatatgt
     7741 tattttcttt ctcatcgctc caggtgtacc ttctctttat aaaaaaacaa attatgcggc
     7801 taagaagact cgaacttcca cgaggtcacc ctcactagcg cctgaagcta gcgcgtctgc
     7861 caattccgcc atagccgcat cacatctttt agtttaggat tacatgtgtt ttttgtcaag
     7921 aacatatttt tgttcttttt catttctgta cgccctccct tatgccatct acacaaataa
     7981 ttcatccgag caactccatc atctattgag ggttggtgaa aaaatctgtt ttatccatat
     8041 gatcgatttc aatgttaaac gaacgactct gggattagta actattttat ttttttcttc
     8101 atttcttttt tcctcttttt ctcgattcag ctatcttctt tcatttggat attgattatt
     8161 tcttctgttg attactatcc ttctggaatt ctagacgctg aagtaattca cgttttcttt
     8221 cttctagaga tcataagtaa cgaatgaacg aacttcaaat aattttttta ttgtaattca
     8281 tatttgtatt gttcgatgat tcttaatacg atatgacaca ataaataaaa ataggcatgg
     8341 ctatttacaa caatagtgct atagtagaaa acaaatgaat aaggttactt aaagaggtga
     8401 atacgatgaa actgatcaaa ctatgggaag cagatttaaa gaaagcttat cagctacaaa
     8461 ctagctttgg accaaatgag agtggattta tcaacaatgc ttatggctat acatttgctg
     8521 aatttttaga ttatgtaaaa aaatgcgaaa aaacgtcttt aggaatcgat cttcctgaga
     8581 gttacgttcc cgcaaccgtc tttattttag aaagtgatga acatgagtat gtaggaattt
     8641 ttaatttgcg ccataaactg aacgaaacat taagaaacgg cgctggccat attggttacg
     8701 gcatctcccc taattatcgt aaaaaaggat atgccacaca aggattagca ttagtactac
     8761 aagaggcaaa aaaaatcggc attgatgagg cacttttgtc tgtaaataaa aaaaatcttg
     8821 gcagtcttaa agcacaacaa aaaaatgggg ctgcgattta tcgtgaagat gagaaagaat
     8881 actatacgaa aatcaaactg atgtaaaaaa gaaggattcc aaaaggaaag ggatttgaat
     8941 gtcctttatc aatagaacac atttatttaa ttaacttata atatagcaga caaaatttta
     9001 taaacaaaga aaccttttat ctcttttctt ctagaaagga taaaaggctt ttttgttttt
     9061 cctatttgct tctcatagct agtcagccaa gatttctatc gtatccccaa cgcaatccgt
     9121 gcataacggc tcatcttatc tgtcgtccat gctggatacc agactagttt gacttctgct
     9181 ttcgttactt ctggcacttc ttttaatgcg tcatggatag attctgttaa aatatctgct
     9241 aatggacagc ccatagttgt tagagtcatc ttaatgactg tttctccatt ttcaggattg
     9301 aattctatat cataaatcaa gcctaaattg acgatgtcga ttcctaattc aggatcaatc
     9361 accatttcca aggcagcaag aatacgttct ttgattagtt ctatttcttg tccagaccgt
     9421 tcttggtttg cttcgctcat cgtacgacac tccttaacac gatttttatt tatcatactc
     9481 tttttttagc tgcttgtaaa tgagaattta gaagtaagat tctc
//
LOCUS       DACDIG010000090         9416 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.090, whole genome shotgun sequence.
ACCESSION   DACDIG010000090 DACDIG010000000
VERSION     DACDIG010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.090"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..367)
                     /locus_tag="IV247_002472"
     CDS             complement(<1..367)
                     /locus_tag="IV247_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8778499.1"
                     /translation="MINFSIKELNAFAVIGQEVELTNYQKKNIQISTQFWRKFNSSLK
                     KAYLSQSGNWVKYAFMERRNGKLYYFCSIPQRTITPEGFLYKEIPSYKYLVVEHIGAM
                     DKIYETYGKIYEEIIPSTSY"
     gene            complement(364..606)
                     /locus_tag="IV247_002473"
     CDS             complement(364..606)
                     /locus_tag="IV247_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HAP8778500.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNCIITVNAYSYTVITAHKVK"
     gene            complement(1152..1682)
                     /locus_tag="IV247_002474"
     CDS             complement(1152..1682)
                     /locus_tag="IV247_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8778501.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
     gene            complement(1864..2520)
                     /locus_tag="IV247_002475"
     CDS             complement(1864..2520)
                     /locus_tag="IV247_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAP8778502.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            complement(2647..3120)
                     /gene="dfrF"
                     /locus_tag="IV247_002476"
     CDS             complement(2647..3120)
                     /gene="dfrF"
                     /locus_tag="IV247_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HAP8778503.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            complement(3539..3757)
                     /locus_tag="IV247_002477"
                     /pseudo
     CDS             complement(3539..3757)
                     /locus_tag="IV247_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3788..5002)
                     /locus_tag="IV247_002478"
     CDS             complement(3788..5002)
                     /locus_tag="IV247_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAP8778504.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(5343..5585)
                     /locus_tag="IV247_002479"
     CDS             complement(5343..5585)
                     /locus_tag="IV247_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP8778505.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(5617..6174)
                     /locus_tag="IV247_002480"
     CDS             complement(5617..6174)
                     /locus_tag="IV247_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP8778506.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(6187..6375)
                     /locus_tag="IV247_002481"
     CDS             complement(6187..6375)
                     /locus_tag="IV247_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP8778507.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(6388..6954)
                     /gene="amaP"
                     /locus_tag="IV247_002482"
     CDS             complement(6388..6954)
                     /gene="amaP"
                     /locus_tag="IV247_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP8778508.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            8184..8657
                     /locus_tag="IV247_002483"
     CDS             8184..8657
                     /locus_tag="IV247_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAP8778509.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            8666..8893
                     /locus_tag="IV247_002484"
     CDS             8666..8893
                     /locus_tag="IV247_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8778510.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT         2878 a         1847 c         1368 g         3323 t
ORIGIN      
        1 tatacgatgt actaggaata atttcttcat aaatcttgcc ataagtttca tatattttat
       61 ccatagcacc aatatgctcc acaaccaaat atttataaga cggtatttct ttatacagaa
      121 agccctctgg ggtaatggtt ctttggggaa tagaacagaa ataataaagt tttccatttc
      181 ttctttccat aaaagcatat tttacccaat ttcctgattg tgaaagatac gcttttttca
      241 aactactgtt aaactttctc caaaactgtg tactaatctg aatattcttt ttttgataat
      301 tagtcagttc tacttcctgc ccaataactg caaatgcgtt taattccttt atagaaaaat
      361 ttatcatttt accttgtgag cagtgataac ggtataactg tacgcattta ccgttataat
      421 acaattatca atcgttatat accagttttt tccttttctt gtgatttccg cattagaaag
      481 gctaattttt tctttgcacc attcaattac attttctaca ttcaaagata agttcttttt
      541 aattctaata acgccaagtt cagttgtgtg aagtcttcct aagttttcca gtaattcatt
      601 ttccatattt tgatacctca tttttcagca aatttatttg tatgttttat accccaatta
      661 taacattctc atatcagcac tacaataaaa ataattaagc accaataatc ttattgaaca
      721 tctccaaccg taaatctttc acaccatcag tgataaacac tgagctgaca gcaattacga
      781 caactaccat aattgcctgc acttaaattt ctgcaaaaga aaacgacaat caatttctta
      841 ttttttgaaa tcaactgtct ttctcatatt tactattcaa ttttcatagt gatataggta
      901 caatttatat cactactgta aaaatggggt tttgccttat aaatacagcg ttttccgctt
      961 gttagtgtat agtgtttctt gttttttgtg ccccccttgt tagataacgg gggctttgaa
     1021 cggctcgcaa gctcgccgaa ccgccgaagc gaaccgcccc cttgcaagca tggcggaaaa
     1081 atcttaagga ttttcccgcc aacgtggtcg gatcgctccg tcggtttaac agcttttctg
     1141 tttctctcta gttatctacg catataaatc aaatcggtta ttccgttata agtctgtgtt
     1201 ttacaaagcc ttaactttat ttccctgtct atgttgccca aaagacgaac cccatttcct
     1261 aacagagtag gaataacaga aatgtaatat ttatcaatca tatttttgct taccaactgc
     1321 tgaacaagat ttgctccgcc acaaatccaa atatcctttc ctgcttcttg ttttagccgt
     1381 tccaataata aaacaggatt ttcacttgta aaatgaattt gttgggaaga attgcactct
     1441 ttatgtgtta atacataagt tgtaaattta ttatataccc attcttgcgg agaaagttca
     1501 gtaacaacct gatgagaagt attccacccc attaagacag tatctatatc tttggtaaat
     1561 tctgtataag tgtcgatgtt ttcattgtca tttccatgac catttagcca agctactcca
     1621 ccattaccgt ccgcaatata tccgtcaagg ctcatggcaa taaataaaac tacttttctc
     1681 attttttctc ttttataaat acaaacagga atttatttct cttttttact cataagggaa
     1741 caactgattg ctacaaataa acccattaaa atccatggaa atgctgctct tataaaatca
     1801 ctaaccatac tttattcctc cttaaattcc aatttcctta gttctacaaa ctgaaatttt
     1861 tgcctatgaa tgtatcttgt tctttatacg caaacaggct tctgtgtgca taatccaatg
     1921 tcttgaaaac ggcatttgaa ttagtccaca aatatcttta ttacaccaat aatcaatcag
     1981 ccaaattgct ttttcattgt cgtttactac attcaacgat tctaagtatc cttttctttc
     2041 tttcggtatt tttcttttca attcatcata agataaccgt tcaagcattt tctcagtgct
     2101 ttcccatact tcgaaaatat ataaatataa ttcttccaga ttaagctgtt ttgaaaagtc
     2161 tgcaatctgc tgtttcatga gttcatttcc tgttgtaatg ataggtgaat tgatacgctc
     2221 ttgataattg cctgcaaaaa atacttgttc atcttcattt atcactgtat gcacaactat
     2281 atcttcaatg cggaaaatat gccaaataga ataagcaatc gtcttactat gataaccatc
     2341 agcgtttatg aaggggattg agttaaaatc ttctctgcat aattcttctt tgaaagatac
     2401 taaggtttgt attaactgac ttcgtaaagt aagtaaagta tcaatacccc tcttataagt
     2461 atctttcttt tttatttgtg cctgcattgt tttattaagt tcagaccatt ccttattcat
     2521 attcaatcct ctctcaaatt ctgattttat cgccccatac aaactggaat ttgtcttaca
     2581 acaatttata tatgcataaa aagcaatata tttatgaaaa caccctaaat ccaaaattta
     2641 ctcaattcat tttttcctta cataaaatgt tctcgtatat ttcacttcct caccaagtgt
     2701 ttcccctatc aaaacttcaa aatcattgat atcaaattct ggaaagaatg tatctccatc
     2761 ttcaatattt aaatctactt ctgtgatata cattttatct actatttgca aagcttcctt
     2821 aaatagtcca tatccaccag atatgtatac atctcgtcct ttagccaata ataatgcatc
     2881 ttctaatgat ttaactgaaa ctaaattatc tccttgatac tctgttgtgg tggaaacaac
     2941 aatattcatt ctattaggca acggatgacc gatttcttca taagactttc gccccataat
     3001 aaccacatta cccgttgtta actctctaaa ttgcttttgt tctcccttta ttttccatgg
     3061 tatattacca ttcttgccta taacattatt ctttgacctc gcaacaatca aacctatcat
     3121 ctatttcatc ctccataata tgatgtcgta cctgtgattc catcaagtgc accacatctt
     3181 aacaaactca aaattatctc gaactaacca gctcgacaaa ttcctttttt gtcttcatct
     3241 gcattatatc atattgttat aagcattgcc atttcttttc tgcttttgaa aagttactta
     3301 tcagcctatg ttttgtaaag ggtgatattc agcatttctc acgcaacaag cccttgacaa
     3361 accatagact aataagtttt aaggtaatca agcagaaatg aaatagtctg ctgtttcacg
     3421 gcaacaaaaa agagatacca acacacttat gtagtatctc ttagtctagt agcaatattc
     3481 ttttgtttgc aagcaggtaa acatgggctg tttcttaata tctttttgtt tgggaaacac
     3541 ctttttccca tcctttcacc gttgccatct ctgctgcgca caatgccaca gggataaacc
     3601 atagcaaacc gcctaataac aagaaaaata ctaagctgaa gcctgaggtc gcgaatgtgg
     3661 gatattcata taatgaaacg accattgaga tggtcatagc caaaaagcca aagaacgtta
     3721 atttttttgc aggaattttc gttttgcttt gttgcatgaa tcttcctctt ttctatttga
     3781 atttgtttca attgagtttg ttgccaaaga agaaaaagtc caatccttct tcttgttctt
     3841 cttttgtagt agtgatcgtt gtaaagtgca tcgccgattt taaaatcact gttgtcagca
     3901 cataggcata cacggcagta atgatgacac caatcacttg aatccctaag aagtaccagc
     3961 tgcttggatt cgttagtaaa tggctgttag atgcaaagat ccctatcaga atcgtacctg
     4021 tgaatccgcc catgccatga acaccccaca catcaagtgc atcatcccat tgtttctttt
     4081 tacgaaaatc aacagcagca tgacagacga tacccgcaat gatcccaatc agcatggcac
     4141 tttttgcttc tacataaccg gcacaaggcg taatggctgc caatccagca acggatcctg
     4201 ttagtacgtc gacaaaatca aagccatttc cttttacttt tgcaatgatc agccaagtaa
     4261 tcatcgcaaa tgcaagagcg atgaatgttg aaccaaatgc attgactgct tgttcatctg
     4321 ctcgaagtgc accaccggaa ttaaaaccga accagccaaa ccaaagcagt cctgtaccga
     4381 tagcagctgc cataagactg ctatgctgac ttctttctcc taaaggaatc tttctttgac
     4441 caagtgtgag gatacatgct aaagagccga atccagctgt cgtgtggatg actgctccac
     4501 cagcgaaatc tctaaatccc atctgatcca aaaatccgcc accccatatc cagtggcaaa
     4561 cagggaaata aatcagcaga ttccaaaaaa taacaagtat aatatagcct tttaaattca
     4621 atctccctgc aaaagctcct gtcatcaaag gaacggtaat cacgataaac atcagttggt
     4681 aaagaaagaa cattaaaaat ggaatcgttg caccatgaaa catgttgggg aagcttgtaa
     4741 catgtctcat caaaaagaaa tctgccgggt ttccaataac accgccgata tcccgtccaa
     4801 aggccaatcc gaatccacca aatacccaca taatcgttgt tatcccaacg gagagaaaag
     4861 attgagacat gatcgttagt atattattct tccgtgctaa tccgccgtaa aaaaaagcta
     4921 gaccaggagt catcaggcaa accattgctg tacataaaat catgaatgct gtatctcctg
     4981 tattgatagg taatgtattc atttttttcg cctcctttct acgattacga gtataaaaga
     5041 aggtgtagtt gtttttttct tctgtttttt tgaattaata cttgcattta taaaagacag
     5101 acaattaata aaagcttgat gtgttgtgct ctttttcgtt tttcacacaa ataaagacaa
     5161 aaaataaacg gtcttctttt tatagaaaac cgtttatttt cgtctttttt tatttttaaa
     5221 gatatttttt cataaggaat aaagaatact tcatataaaa tatataaaaa agaaccgcag
     5281 cgagtgtcgc tacggttctc aaatttgttc ttcatttcaa cataagtttt tcttcaattt
     5341 gcttataatt tacgtgcaaa gaatgaaacg actaatacta aaatgattgc accgatcaat
     5401 gatggaatga tagccattcc tgcaattgtt ggtccccaag ttcctagtaa agcttgtcca
     5461 attgctgaac ctactaaacc agcaatgatg tttgcgatga tccccattga tgatccttta
     5521 ttcgtgattg ctcctgcaat tgctccaatg ataccgccga cgattaatgc ccaaataaat
     5581 cccattttaa attcctccta actgacttct tacttcttat tgtacacgtg gttcgttatt
     5641 ttctttgact ttttgtgcac ctttgcctac agcagatttt gctttatctg tttggcgtga
     5701 agcaaattgt cctgtggatt ttgctgcttc tgttactttg tcttgtactg tttcttgatc
     5761 tttttggtat tcttcttgtg ttttgatgtc tgcaacattc acattcactt cgataacatc
     5821 taaatgtgtc atattgttta cttcagtact gatcaattct ttgatttggt tgtaaatatc
     5881 ttcgatgtct ttaccgtatt ctacgatcac atctagatcc acagctactt gtttcttacc
     5941 tacttctgta ttgatacctg ctgttacgtt atctgtgttg actaattttt cagccatatt
     6001 tgagaaaaat ccgccatcta atgtcaatag accatctaca tctttcaatg cataaccgat
     6061 gattttttgg atgactttgt cttcataagt caattcacca ttcatcatgc ggttttctcc
     6121 tttttcattt gccaaaactg atccatttgt tgtattcatg tttttgtttt ccatctttgt
     6181 ttcctcctat gatttaaaaa attgatcaac tattcctgac tgtttcaaat aaaaaccgat
     6241 tcctgcgcca attgctgtca tgattatgat caacaatgtc ttgaaaaatc caatcgttaa
     6301 ccataaaaca gctagcagta gtccaagacc cccaaagata atagggagct tgtatgctga
     6361 aaatagttct ttcattattt ggccctccta ttccactcga gagtgcttgt agtctcttgt
     6421 atcttcttgt tcttcataac cgctgtatgt gactgccact ttgaccgttt cttttgttcc
     6481 gagtacttta gttacttctt cttcgatatc ttgcatcaat acacctgttt tccctactaa
     6541 tgaagaagtt cgtttcaagt ctccgttgac atggatatgg attcgatttt tcgttgaacg
     6601 tatccgtact ttaggtgaat gaatgaactc ttcttcgtgt aaatgggaac gtaccaaccc
     6661 ttcgatcgct tttttgtcga ttgttagttt gccatcttcc cgtttcataa cgaaagttcc
     6721 tcttgacttt ggatagaaaa gaacgactag caagatgata atagcaagaa cggcaaatac
     6781 tacagctgcc cagaaaatat attgttgcat gtagtaattc gttaaagtaa agaagcgaaa
     6841 tcgttccaat tgttcaggca taatatacgg cgcattgatc aacgcaatcg ctagaaatac
     6901 tgaaagtagc agtaaactca tgataacacc gattattttt aaccccttat tcattttcca
     6961 cctcctataa acttattttt accctgtaaa taactctacc gacaatcaca gtatagatag
     7021 cgaacagtta gcatagcatc agtaaataaa gcgctaccaa acattgctgg atcataacgg
     7081 cgttttattg ccagtctgat actgaaataa caattgctta cttattctct atttactttt
     7141 gattgctggc tatacttaaa ggatattatt caagattaaa aattgcaaag attatgctca
     7201 gttattatta caagattcta tttatttatt aaattttttc ggagaattaa aaaatacaat
     7261 aaataacttt agttacttta atgcgttctt actttatttt tatattatca tttttcttat
     7321 tcacttcttt ggtttatact attcttataa ataaaaacga ggtatgtcct atgatgaaaa
     7381 gattgtacta ttcattgatc ataacgattg gctatttgat tgtatcaaat ctaggaaata
     7441 tggtatttgg tatcagtaaa gaattcagtt ggactactac attatgggaa tctctattct
     7501 ttttcatctt cgtatttttg ttacaaaatt atcgaaaaaa ataaacatgt gccatctatc
     7561 tgttggcaca tgttttagga tttatggtac tttttattcg gcaatccatt ttatttttct
     7621 acttcttctt aatacctcta acacttttga tttataaact aatccaatgc atctacttgc
     7681 gcttattaag ctatttcagc tagatagtac caccccatgc gtcaatgctt gattttcctt
     7741 ttgtataaca ttgtataagc aataaaggac aaataagtgg tgattacacc aaaccattga
     7801 ataagaaaaa ttctgatacg taaataaaca tgaacttgaa aaaattaatg aatcggttag
     7861 ctactacgta gttgtctttt ttctcataca gttctgttaa cttacacttc tcttcgctag
     7921 ataataaaaa atcctccaac tgtattgtcc aatataatcc caggcatcta aactagcatt
     7981 atagaagaga cacacataga atctaaaata actggtttta aatgtttctt acaatcaaaa
     8041 aaagctgtta aatgcactcc acaccttcat actctatcat tttttataaa cgatatattt
     8101 ttattgtttt tcgataaaaa atagttgttt tacaataatt attctgctat attttagcta
     8161 tcaaagataa aagagggata aaaatgaaat ttaaaacatt gctattaaaa tttgtcgttt
     8221 taggtataag tgccgtattt attttgttcg gcgtactttt ttatactcag atcgctacaa
     8281 gcgaaaaagg attcaatttt gattggcaga ccacattatt tgtttctgtc atttactttg
     8341 cccttctcct agggatcgtt attgcttatt tcttattgaa actattgaaa aaaatcgaag
     8401 ataaccaagc tttttccact caaagcttaa acatactaaa aaaaatcgaa cgaaccattt
     8461 tgtttattgg tttcgccttt tttggcttac ttcctaaatt tatgaaagta gcggatttag
     8521 acgatgctcc aggtttgatg ttggtaggaa tcgctgtcgt attcattcct tttgcagtat
     8581 acacattaac aagcgtgctg gaaaaactgc ttgaacatgc gattttactt aagacagatc
     8641 atgatttgac agtgtgaggt gcgacatggc aattatcgtt aatttagatg tagtattagc
     8701 tcaaaaaaaa atgagtgtga ctgaattgag tgagaaagtg ggcattacga tggcaaacat
     8761 ctctattcta aaaaatggaa aagcaaaagc catccgtttt tccactctcg aaaaaatctg
     8821 ccaagttttg gagtgtcaac caggtgattt gttgacctat aaaaatgact cgaatactat
     8881 accagaagaa taaaataaag tagaacaaac agtaaataaa aaataatctg aatgatagat
     8941 gtgtgagact tttagaagtt atttacgtct attattcaga ttttttattt cgattgaata
     9001 ccgttttatc tataaataag atatgtactg actcatacaa agggggcctt ttcttctcca
     9061 tgtactatca cttttcctga tacaactggt ttcctgttac gttgtagttg gatctgcttt
     9121 tagtataata ctttattcat tccaccagac tgatagcagt ttgagcagtc taaataatca
     9181 tgtagtatta caggccattc tgtttcatac cttgaagtgc ttaaaacttc agctaatggc
     9241 ccattgtgtt aactcttact ccattctttt ttagtaagct cccatatata ctgtaaagcc
     9301 ttctatatat tatgtcatgt tgctttttaa catttactat gtagttttga taaaatgatt
     9361 tgaataacag tgatggacaa ttctttttta tttttaacat tattttgata ataatg
//
LOCUS       DACDIG010000091         9041 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.091, whole genome shotgun sequence.
ACCESSION   DACDIG010000091 DACDIG010000000
VERSION     DACDIG010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.091"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            75..148
                     /locus_tag="IV247_002485"
     tRNA            75..148
                     /locus_tag="IV247_002485"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:109..111,aa:Ile,seq:cat)
     gene            complement(533..1030)
                     /locus_tag="IV247_002486"
     CDS             complement(533..1030)
                     /locus_tag="IV247_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8778511.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(1070..1879)
                     /locus_tag="IV247_002487"
     CDS             complement(1070..1879)
                     /locus_tag="IV247_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8778512.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            2213..3070
                     /locus_tag="IV247_002488"
     CDS             2213..3070
                     /locus_tag="IV247_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP8778513.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            3063..3965
                     /locus_tag="IV247_002489"
     CDS             3063..3965
                     /locus_tag="IV247_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP8778514.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            3998..4348
                     /locus_tag="IV247_002490"
     CDS             3998..4348
                     /locus_tag="IV247_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP8778515.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            4382..5320
                     /locus_tag="IV247_002491"
     CDS             4382..5320
                     /locus_tag="IV247_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP8778516.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            5398..6291
                     /locus_tag="IV247_002492"
     CDS             5398..6291
                     /locus_tag="IV247_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8778517.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            6329..6892
                     /gene="amaP"
                     /locus_tag="IV247_002493"
     CDS             6329..6892
                     /gene="amaP"
                     /locus_tag="IV247_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP8778518.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            6906..7094
                     /locus_tag="IV247_002494"
     CDS             6906..7094
                     /locus_tag="IV247_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP8778519.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            7107..8009
                     /locus_tag="IV247_002495"
     CDS             7107..8009
                     /locus_tag="IV247_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP8778520.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            8041..8283
                     /locus_tag="IV247_002496"
     CDS             8041..8283
                     /locus_tag="IV247_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP8778521.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(8539..8910)
                     /locus_tag="IV247_002497"
     CDS             complement(8539..8910)
                     /locus_tag="IV247_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778522.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         3145 a         1558 c         1753 g         2585 t
ORIGIN      
        1 atattttgaa acttgtgaga gtttgtcatt cgtgctatag ttgaaaagta acaaaaataa
       61 attttattta tgatgtacct ttagctcagt tggttagagc agacggctca taaccgtccg
      121 gtcgtaggtt cgagtcctac aaggtacata agaggttgtg aaaaagctgc cctgcatcaa
      181 gtaataagaa cagccaagta aaaacagctt cttatgtttt tacttggctg ttcttattac
      241 tgcagatgca agcttttgaa taccgtttaa ggaagaggtt gtgaaatcag cgttttgacc
      301 tgagtattag cttaacaaac ggaaaaatag ttcctcatat tttgccgttt gttaagctaa
      361 taccgagggt cagctgattg aacaccattt aagaaagagg tcgtgagcct gccgtttcac
      421 tctgtgtcat aaaaaagcca atctactttg atagaaaaag cacgtaggac taaaacagtt
      481 ctgcgtgctt tttttattgt gtgttaaata tcgtaaatga agatagatat ttttatgatt
      541 cgacgataga gacgttgaaa tctttcaaca gctctaaagc ggattctccg tgttttctac
      601 tggagactaa cattggatca acaaaaaagt ctgcttcagg aaaagcactc tgcaggccta
      661 tcagattaga gagtacacac aagtttgttt caatcccgac tacttcgatc tctttttgtt
      721 cttctttgct ttcaaataaa gtttcctgga tttcagctaa agtttcaggt ggaatcgtaa
      781 agtaatactt tttaatcact cgttcatttt cttgtggaga aagaagaggg atcaattgca
      841 aatcttctcg ttcttcttct ttgtccttca gttcaactgg tatatcccgt gtgaagagaa
      901 tatattcatt attttggcgt gctttagcta atctttgagc aatacgttct gccaattgat
      961 ctgactctgg aatataaaat tcgctgtttg gatcaagaat atgattttgc atatcaataa
     1021 taactaacat tttctttcct cctacattca tatcgtcatt ttattctttt taatagaagt
     1081 tttttaaaat ataagcaaca ccagcttctt cattcgtcag tgttacttgg tttgcaattt
     1141 cttttacttg tcgctgtgca ttccccatcg caatactcaa cccgctgtaa ttcaacatag
     1201 caatgtcgtt ttcttgatcg ccaaaagcca tgatgtcact cgattcgatt ccaaggtaat
     1261 ccgccagaaa aacaagtgaa tgagctttag tgatacctgt aggcgtgatt tctaagaaat
     1321 gcggatcaga gaatactgat tggataccat ctgcttgcag atctctttgt agcttctgta
     1381 gtttttcttc actcttatct gttaatgcta atttaagata gctaagagaa ggatcttcca
     1441 acatatttaa aatttgatca aaagaatggt tctgtaaatt gaaatgttca taataagggt
     1501 catctttgac tagatttgat tcatcgaatg tcagctgatc atcttgggtt tctcggctag
     1561 caatgatgga tattttcgct tcttgagcat agaagaaggc ttttctcagt gtatcgctat
     1621 ctatttttga acgtctgaga actttcgcca tttttccatc cttcatttca gcaatcagtg
     1681 aaccattgga agaaatcaaa tagccaccga ttcctaattc ggaaaaaata tctttagcag
     1741 aaatgctgtt ccttccggta gcgacggtta cgattccgcc attttcatca aatttccgaa
     1801 gtgcttggat attttcttct ggtatctttc catttcgatc aagcaaggta ccgtctaaat
     1861 caatcgctgc tagtttcatt ttatcttcct actttctttt tacatatcta ttttcctgtt
     1921 accttaagat taaatgagaa tttaaaaaaa tgaaactaac ttgctttgtc attatgaatt
     1981 ttttcaaaaa acattcttaa gaaaaaggtg gctatatata gtagtttttg gtcaatacct
     2041 ttgaataaaa agccatgaaa aacgaatagt tagcgcttac cgatgcgttt tgtgataaaa
     2101 aaattttaaa atattcaatg aaaacaaagc atatattttg ctttaaaacc aaatgaggaa
     2161 tattctagat gtagaacagg ttagaaaaag attagaaaaa ggagtgattt tgatggagaa
     2221 gagatacggc gtagttcctg tggtaactgc ttcagacgaa aattatgctc cttacttgag
     2281 cgtgatgatc gcaactgcat tagaaaattg caataaaaca agacgcataa aattttatgt
     2341 gattgacgat ggtctatcag aatatagtaa agaaggattg gaagaaacgg taaataaata
     2401 ttcaagtaat gcaagtatcc aattcctgac agtggaaaaa gatatctacg aagacttcct
     2461 agtcagtgac catatcacaa ctaccgctta cttaagaatt tctttaccaa acttattagc
     2521 taaagaggat tataagaaag tcctttactt agattcagat gtgttagtac tagacgatat
     2581 cgtaaaacta tatgatgagc cgttaaatgg aaaaacaatc ggcgctatta tcgatccagg
     2641 ccaagtgaaa gctttggaaa gattaggaat cgattccgat gacttgtact tcaattcagg
     2701 agtgatggtg attgacattg atcaatggaa taaaaaagaa attacagaaa aaactatcca
     2761 ttacctcagt gaaaatggcg atcgaatcat ctatcacgac caagatgctt tgaatgctgt
     2821 actttatgaa gattgggaac aacttcatcc aaaatggaac atgcagactt cgctgatttt
     2881 tgaacgtcat ccagcaccaa atgaaaaata tgaacgtcag tataaagaag gaaatgaaaa
     2941 accttcgatc gttcacttta caggacatga taaaccttgg aatacgttga aagatcatcc
     3001 gtataccaat ttatacttaa aaaaattagc acacagtact ctaacgaaag taggcgagat
     3061 caatgaataa aaaagaaata gcagtagtcg caagttgtaa cacaaaattc gtaccacatt
     3121 tagctgcatt atttgtctca gtattagata attgcaaccc gtctaaattt gttcggttct
     3181 atgtgattga cgatgatatc gattttgaaa gtaaacagct tttacgcttc tcagtgaaaa
     3241 acgcacgaat gaactcagat gtggaattct tgaaaatcaa taaagaattc tttacaaatg
     3301 tggtaatcag cgatcgtatt ccagaaacag cttattatcg tattgcgatt ccggaattgt
     3361 tccgcggcac agaagttgaa cgtatcttat atatggattg cgacatgatc gctttacaag
     3421 atatctctaa attatggcgt cttgacttcg gtgattcaat cgtagcagct gttgaagacg
     3481 ctgggttcca tcaacgactg gaaaaaatga aaatttcagc taaatcaatg cgttatttca
     3541 actcaggttt gatgctgatc aatgtgaaaa aatggttaga tgaaaatatt acgcaaaaag
     3601 tattagattt catcgaacat aatccagaaa aattacgatt ccatgaccaa gatgcattaa
     3661 atgctatctt acatgaccgt tggttaccac ttcatccgcg ttggaatgca caaggctata
     3721 tcatggcaaa agcaaaaaaa catccaacag cagcaggtga acgtgaatac gaagaaactc
     3781 gcaacaatcc ttacattatc catttttcag gacatgtgaa accgtggagc aaggattttg
     3841 aagggccaac gaagaaatat tacgaaaaat atgcagggat gacggctttc cgttgtgtag
     3901 ctaaattccc taaatatcca aaatatgcaa aagttcaacc aaaaacagat gtagttgtta
     3961 aataatagaa gacaacaact gaagaggggg agtaggaatg gaaattacaa tgtctaaaaa
     4021 tgcagtagag acgttaattg aaaaggttgg ggaaaataca aaaatcgcac tcgcgcttat
     4081 caatgattcc gatccatttc taagggacaa aggtgctttt gcaaaaggca gcttttttca
     4141 aatcattcct tttgtttcgg aatttggaga atatgcgact aaaatcgagc atccattatt
     4201 ggacatctat acatctaaat tagagcaaaa ttatttcggt aaacgattga acatggactt
     4261 caacaaacag cttgacagct tttcattaga aaatgaaatc gcagtactcg attataatat
     4321 caaattaaag aactgcttct tcagctaata gtttcgagaa agtccaaaaa ggaggtatac
     4381 attgagatac tactttgtgg atgaagaaaa gaatgtaata gaaacagatg aaaaaaaata
     4441 taattggctc gtacttgata gtgcaaagcc cgaagagatt gagcaagcaa tcgaagaatt
     4501 cgagcttcct gatgatattt tcgtaggaac cgaatatccg gaagaagttt ctcggatgga
     4561 gcgcctttat gagacgaaac tagagcatcc attttctttg gtagtgatta atctagatca
     4621 gtcagataat cgtatcgaaa aaaaattaac gccaatatct tttgtacttt ccgataatct
     4681 cttgattact tgtatcgata gaaaaaccga atttgtggat cggctgctga caaagcatgg
     4741 aaatcattta aactcgtttg aaaagatcat cacgtataca ttattagata tttacaccca
     4801 ttacgtgaaa gaactaagag agatgaaaaa gcgaattgac gctttggata aagcagctcg
     4861 taaaacaacg gaaaatgaag aactgtataa acaggcagat cttgaaagag acatcgtata
     4921 tatcgatcat accttaagag atcaacggga aacaatggat caattatgga attctgaaac
     4981 tttcatcaaa cgtttagacg atgaacagct actctatgat gtccatttga gacagcgaca
     5041 aacagaaaaa atgatcgaga tctatcgaga tttattagag agtattggtg atctattcaa
     5101 cgggatgatg gacaataatc taaatcatat catgaaatat ctagattcag ctgcattaat
     5161 catttccgtt ccggcgttgt tcgcagggat ttggggaatg aatacaggag gattaccggg
     5221 taaaagttct gctctaggct ttatactcgt agtctttggc tctttgatag cagcagtagt
     5281 tttaggttac catttatacc ggaaagatta tacaaattaa agaatctatt taaaacaggt
     5341 atcaaacaaa gaaatttgtt ttggcaatac aatttaaaaa ctaggaggaa tacagcaatg
     5401 acagaaccaa aattaatcga cccgcgaaac ttgtaccata cggagaaatt tcctaaacaa
     5461 gatcaggata caccagcttt acagcagaat atgattcctg ttcctgattg tggagaagaa
     5521 tcctatgaag gcaataatca actagaaaac cgccgtgtac taatcactgg tggagattca
     5581 gggatcggtc gtgcagcagc gattgcattt gctagagaag gggcagatat cgctcttcaa
     5641 ttcttcccag gagaagaaaa agatgcagag gaagtagcaa aatacgtgaa agttgcagga
     5701 cgtaagattg tcttactacc ttttgatctt cgtgacgaac aagcacctaa agaaatcata
     5761 gataaagccg tgagtgaact tggcgggcta gatacgatgg tactaaacgc aggacagcaa
     5821 atttcttgtc agtctattgc tgagttacca cttgagcaag tcaaagacac atttatgata
     5881 aacatcatcg ctatgtttgc tttagtgaaa gaagctgttc cgcatatccc agcaggagga
     5941 gcaatcgtaa caacaacttc tgttcaagca ttcaatccaa gcgaacattt attagattac
     6001 gcagcaacaa aagcttcaat cgctaacttt acgatcggtt tagcaaaaca gcttgcacct
     6061 aaaggaatcc gtgtaaatgg tgtagcacct ggtccaatct ggacaccatt acaactagac
     6121 gaaggacaac cttctgattc gatccctgaa tttggtcagc aaagtttact agaacgtgca
     6181 ggtcagccag gagaattagc accagtttat gttttcttag catcgaataa agcaagttat
     6241 gttacagccc aaatttacgg agtaactggt ggagaagcga tcaacttata agaagttgat
     6301 cgtttcaccc ctatgaggag gtgcgaaaat gaataaaggt gtaaaaacga tcggagtgat
     6361 cggaacatta cttttgctat ctgtgctttt gtttgcgata ctattaagtg caacctatcc
     6421 attgcctttt cagctagaga agtttcgatt cttttccatc actaattcat acgtgcaaca
     6481 atatgtattt tggcttgccg ttgcatttgc tattttgaca attatcacgt tacttgtttt
     6541 cctgttttat ccaaaaacaa tcggaaactt tctactgaaa catggtgatg gggagttgac
     6601 gttggataaa aaagcaattg aaggaatggt tcgttctcac ttgcatgaag atgaattcat
     6661 ccattcacca aaagtaaatg tcaaagcaac gaaaaacaag atcgccgtca atgtaaaagg
     6721 ggagttgaaa cgaacttcag cactcattgg taaaacagga acattgatga aagaaatcga
     6781 aaatgaagta caacacatat tgggaacaaa agagccagtg aaagtatcgg tgaaatacac
     6841 aggctttgaa gaaccagcta aacgaagcaa tgaccgcaaa cgagtagaat agggggggac
     6901 caaaaatgaa agaactattt tcgacataca agctccctat catctttggg gtacttggat
     6961 tgatattagc cgttttatgg atcaccgtgg gctttttcaa gaccttattg atcttgatca
     7021 tggcagcgat cggcgtcgct atcggttttt atctgaaaca aacgaaaatc ttagatcact
     7081 attttaaatc ttaggaggaa acatccatga ataacaataa caaaaataat aacaataaaa
     7141 tgaacaacca aaaaccagtg tctgcacatc cagacccaac gacaccaaac caatcaaatg
     7201 gtgtgaacga tgaaaagaac aaaacagcac cagtatctgc acatccagat ccagtagtcc
     7261 ctaacgttca acaaggagta gaagagaaga aacatgatgc agaaaagcat gcacctcatc
     7321 atccaggaac tccagaatct aaagttccag aacaacatga tgcaatcgat ccttctaaac
     7381 ctgtggaaga gcaaaaacat ccttctgacc atgcgaaagc acaagaaaaa cttagttctg
     7441 accacacaaa cggacaagga aaacttggtt ctgatcatac aaataaccaa ggaaagcatg
     7501 attctgaaca ggaagattca atgcgcggtg agctgaatta tgaagataaa gttgtgcaaa
     7561 aaatcatcgg aatcgcaatt gaacaagtag acggtttatt atctgcaaac ggtggtttct
     7621 tctctaatgt ggcaggaaaa cttgtgaata cagataatgt aacggctggg atcgaaacag
     7681 aagtcggtaa aaaacaagta gcagttgata tggatgtcat tgtggaatat ggcaaagata
     7741 tcgaaaaaat ctttgaagaa atgcaagaag taatcggcaa agaagtaaaa aatatgacac
     7801 atttagaagt gatcgaagtc aacgctaacg tagtggacat caaaacaaaa gaagaatttg
     7861 aaaaagatca agaaacggtg caagacaaag taactgacgc agcgaaaaaa accggtgaat
     7921 ttgcttcgaa tcaaacagat aaagtaaaat cagcagctgg aagcggcgca caaaaagtaa
     7981 aagaaaatac agaaccacgg gttcagtaat aaggaagtaa aacattagga ggaacgaaac
     8041 atgggattta tttggtcact tatcgtcgga ggaattatcg gagcaattgc aggagcaatc
     8101 acgaataaag gttcttcgat ggggattatt gcaaatgtcg tagcaggttt agttggatca
     8161 gcacttggtc aagcattatt aggttcttgg ggaccaagta ttgcaggaat ggcaatcgtg
     8221 ccatcattga ttggtgccat cattttagta ctaatcgtat cattctttgc tcgaaaactg
     8281 taaaaagact tttaaaaaga ggtcgtgaaa aagctgttct tcatcaagta ataagaacag
     8341 ccaaacaaaa atagcttctc atatttttca tttggttggg catattatcg aagatgcaag
     8401 cttttgaaca ctgtttatac ggaataagag gctgtgacaa gacttttgtc acagcctctt
     8461 atttatgcgg gcaaattccc aattagattt ttaaaagttt ttccttaaat gtgtctttat
     8521 tgatacagag aagcttcttt aataaaacaa tgcctcccta aattttcttg agataggaaa
     8581 ggtgaacata caaaataata cgaccacttt gaaaacccag atgatccaca aatggatcgt
     8641 atgatactga tgaagtaaaa agcaatggat cagaaactca aagagattga aaaagacaag
     8701 gaagaaataa tatctctttt ttactcttgt ctgttttagc caataatagc tcgtaaagta
     8761 atctagacaa acaaatagaa gtaaacaaag tttcgtaaga gcagcaatcg tatcatgttg
     8821 ctctgctaga cgattgacga gcaggaaaaa tgttggtaat ggaatataga tcacgagtaa
     8881 tgaaagaaaa aagtaaacaa taaatggcat actattcctc ctaattgtgt gagttcgccc
     8941 tcattttaaa ataacgatat ttgtttgtga agctttttcg tgtcgtaaca aatgttatct
     9001 aatgtatttt taaataaata atcattatta tcaaaataat g
//
LOCUS       DACDIG010000092         8783 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.092, whole genome shotgun sequence.
ACCESSION   DACDIG010000092 DACDIG010000000
VERSION     DACDIG010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.092"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..346
                     /locus_tag="IV247_002498"
     CDS             <1..346
                     /locus_tag="IV247_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778523.1"
                     /translation="IIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFLMSLKP
                     PKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYIGYFPN
                     KNNNFYFYRNKL"
     gene            complement(472..1149)
                     /gene="nth"
                     /locus_tag="IV247_002499"
     CDS             complement(472..1149)
                     /gene="nth"
                     /locus_tag="IV247_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAP8778524.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1156..1854)
                     /locus_tag="IV247_002500"
     CDS             complement(1156..1854)
                     /locus_tag="IV247_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAP8778525.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2122..3306
                     /locus_tag="IV247_002501"
     CDS             2122..3306
                     /locus_tag="IV247_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAP8778526.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3312..3755)
                     /locus_tag="IV247_002502"
     CDS             complement(3312..3755)
                     /locus_tag="IV247_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8778527.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(3981..4268)
                     /locus_tag="IV247_002503"
     CDS             complement(3981..4268)
                     /locus_tag="IV247_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778528.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4439..5791)
                     /locus_tag="IV247_002504"
     CDS             complement(4439..5791)
                     /locus_tag="IV247_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAP8778529.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(6064..6510)
                     /locus_tag="IV247_002505"
     CDS             complement(6064..6510)
                     /locus_tag="IV247_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP8778530.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KVLEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(6746..7672)
                     /gene="cysK"
                     /locus_tag="IV247_002506"
     CDS             complement(6746..7672)
                     /gene="cysK"
                     /locus_tag="IV247_002506"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAP8778531.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(7812..8699)
                     /locus_tag="IV247_002507"
     CDS             complement(7812..8699)
                     /locus_tag="IV247_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8778532.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT         2394 a         1790 c         1448 g         3151 t
ORIGIN      
        1 tattattttt tctgtacttt taagcactac tcttaaagga aaatttatgg aaagaagctc
       61 tcatgcaatg attctcactt ttttatggtt gttgttcacc agaatctata tttttctgat
      121 gagcctgaaa ccgcccaaat ccattagttt atgtttagtt ataaatggta tttttttatt
      181 atcacttatc atttttatta cacctttaga tagatacgtc acttatttat ataatccttt
      241 ggaatactgg acttatttta taggtatatt ggaaggcatt attttctaca tcgggtattt
      301 tcccaataaa aataacaact tttattttta tcgcaataaa ttgtaataag ttaccatcct
      361 agctactgag tatgtacatt tactttttca tgcaaaaaag ataaacgaaa cagcttttct
      421 ccataataac aagataattc atacatttat ttgcaattaa agaaaccacg tctacaggcg
      481 tggtttcttt tttagcgcct tttcttttag acgcatccga tttttcccat cttgacatat
      541 tgatagcaaa ggacatacct cgcacttggg atttcttgcc gtgcaatgat atctaccaaa
      601 aaaaatcagt gtatgatgtg tctttaccca taattcttgt ggtactttcc gcatcagtgt
      661 ttcttctact tccatgaccg tagcatctag tttgcagatc ctcaggcgtt ttgagacacg
      721 ttccacgtgg gtatctactg cgattgctgg tatcccgaat gcatccccca atacgacatt
      781 tgctgtcttt ctgcctacac caggaagaga catcaattct tctcttgacg ttggcacttg
      841 cccatcgaaa cgttcaatca gctgctgtgc acacgctttg atgttttttg ctttatttcg
      901 atacaaacca atcgttttta ttttaagaat gatttcatcg attgaagctt ctgctaacgc
      961 atcgggtgtc ggaaaagagg caaacaaatc tggcgttgct ttattcactg atacatctgt
     1021 cgcctgggca cttaagatca ctgcaatcaa taactcaaat ggattattgt gcttgagttc
     1081 tccatgtgct tcgggaaaca ttccatacat cgtttctaga gcttccattg ttttttgttt
     1141 gcttaacata ttccgctact cctccaacca atttttcagt gagactttcg gcaattcttc
     1201 ttgttgagct gactgttcta cttctctttg aagaagctgc tgttttcttc gtttttggtc
     1261 ttcttccact tgttgttttg tacgtagatt ttttctttcc cacgatagca aaatgcggtc
     1321 aacgtaatta aaactataag cctgatttaa tacggcttcc cgcagtgcta gcttcaaaat
     1381 ttccggctga taatggtctt cttctagcca ttgtccgatc cgctggaact cgatcgaaga
     1441 aagcgggcgt ccaaattctt gttcaaataa ttgatacact gactggattt ctttttcttg
     1501 tgttttttct tcgtattttt tttcttgtgt ttttaaatac tcacctaata gatcatagat
     1561 ggggtaaaga ttatatcgat ctgctttttt accgttgaca atcgtcgttt ctatcttgat
     1621 aaatccattt gttacgagac gatccagtat ttgaaaaatc tgatcttgtt tgattcccat
     1681 atccatagca atcatctgca aatcagggaa agtatctcct tcaagctgtg ccatatgcaa
     1741 ctgtagaaca aataaaaatt cttcagattg tagaccgatc ctatgataat ttttaagtaa
     1801 aagattagat atgactgttt gtccagcgct taagtaattt tctaaattca acatgatttc
     1861 tcctccgctc aatcacagta tacgtaaaaa aaacatggga agcaagaaca ggtttctttt
     1921 ctatttactt cactgatcgg tcagatttac aatgtcgtct acttgatttt tgtttgttca
     1981 tgtttttgat gcgtatcttc acttttcgga ataaataaat taagtagatt tcgctgttcc
     2041 ttagaatgtt gacaaggaaa caaaattact atacactatt tttgtttcca agttaacatt
     2101 attgcataaa ggagttttta catgtacagt ttcacaaata aacattctaa agtttttgga
     2161 tacgtctctg tcttattcac tggattagga tttactatcg tcagtcctgt acttccgttt
     2221 ttaacgctgc cttattctca ctccatacat cagcaagctt tctatatcac tttgctcatg
     2281 tctgtttatg ctctagctgc cttcttatct gcccccattc tcggtagctt aagtgatcat
     2341 tttgggcgtc atccgatcct tattatcagt ttattaggat caagtatcgg atatttgctt
     2401 tttggtttag gaaattcgat atggatgttg ttccttggtc gtatcattga aggattgacg
     2461 gcaggagaaa tctccacctt atatgcttac tttgccgaca tcacggaacc taatgaaaga
     2521 accaaagttt ttggatggat gggtgcttta gtcggtatag gaacaacgct gggtcctatt
     2581 atagggggtc tattagctga gttggggaat tctgttccta tatttattgg agcactcttt
     2641 actttcttaa atgccgttta tggctacaca tttatgctag aaagccttcc aataaaaaaa
     2701 cgttcagtag atttaagttt ctcccatgta agaccttttc accaattaca acaattattc
     2761 aagatttcat ccgttattcc tttattgaca gcaggttttg ctgtttggtt ggcagctggc
     2821 tctcttcaat ctatcttttc tcaattttcg attgacgcct ttcagtggaa ggctgggttg
     2881 gttggtttaa gcttctcttt gattggaatc ttggatactg tctcgcaatt gttcattatg
     2941 cctcgattgc tcaaaaaatt ttcagaacag cagatcacaa gaatagggat gttcagtgaa
     3001 atcctcgcct atctattcat cacattatct ggaattcttc tactgccgat tttgttttta
     3061 tttgggatta tctgttatgg tttcggtgat tctattttta ctcctgtatt caatggccaa
     3121 ctatccaatt ctgtctcaga taaccagcaa ggtcttgtaa tgggcggtac ccaaagtata
     3181 caatctctat cgcgcgtcat cggcccgcta atcgcaggac agctttatgc tgcagcacca
     3241 tttcttccga cggtattcgg atttttgctt atgatttttg cctattatca ctacaataaa
     3301 aaatagtagc ttcatttgct acttcgtata aattctctgt atcgaccgat aaaacgaaat
     3361 actgtgtcaa attcttcttt tgacatattt tcaaatacag tagcatctcg ctgtacccat
     3421 ttttgatgga gttgctcgtg tctattcctt attttttgtc cgattggcgt caattgaaaa
     3481 tagatttctt ttttattctc ttcgctttga taagtgttta tgatccctct agctatcagt
     3541 ttttttgtta gcttactgat ggctcctcta gtcatgtaac tagcattcgc taattttgtc
     3601 acgttaggat cagggatttt cccgatatgt tcaatcactt ctagttcatt tagagtaaat
     3661 cctgcaaatt ctttttccat ttctggttta ttacgatgaa taatctcatt agctaaatcc
     3721 agatattctt ctattagcag ttgtctttta tccatttcta caccttcttt ttctttcatt
     3781 atattgtttt tttgtttcct attcaacaaa gataaaaata ttttgtagga aaacccaatc
     3841 tgaaaccaag aaaaaaaggg gttgacaaag cttgtcataa cccctttttc tgattaaaag
     3901 atagtaaaga gacagtcatc ggtaattgtg atgatttttt gtcatcactt aaggatgagc
     3961 ggccttttca aaaactgtct ttaaagatca tcatcagttt ctagcacata tggctgacct
     4021 tcatatgtgt actctgtatt caatgtgccg tcattttcat ctgagtagat gcgtctactg
     4081 aaaggaaaac gatgagaaac attatctttg gcgtcatttg ctggcatcgt gtaatattcc
     4141 tcttgcgttt cattcaactg agagagtact tgatctgcca actcatcaat attgatcgtt
     4201 aatttcttcg aagcactttt ataatcttct aaaaatgcat cttgttggac aaaatattct
     4261 ttattcatct tctttctcac ctttcttttc aaaatcaaat aaagctgctt gttcttttat
     4321 gtaacagctt tatctttgct gcttattgtg acaaaaaatt cgaagaaaga caactaattg
     4381 tacttttcac aaaaaaagac agagggtcgt gacaaaattg tgtcacaacc ctttttcttt
     4441 attgtgcagt ttcgatttcg cctagcgctg cttgtgcaag taggttcaag taattccatg
     4501 ggcggtcaaa atgtggttgg aagaagaaat ctgaaagtgc gagatcttct acagtcattt
     4561 tattttgaac agctaaagac atggtatttg ctgattgtgt aatgtcgtat ttggacatga
     4621 actgagcacc tacgatacga ttcgttcctt tttcatagac tagctccatc attacttttt
     4681 cagtagttgg catgaactct ggacgataat tatcttcaaa tacagtcgct tggacttcta
     4741 gatcgttcaa agttgcgctt tctttggtca cacctgtcga accgatcgtc cagccaaata
     4801 ggtaaagtcc tgaagttcct tgagtaccac gatacgccat tttatgatct gtcaagttat
     4861 ttcccacaag caaaccttga cgaacggcat ttgttgccaa aggaatatag ttttgcgtgc
     4921 cacttggatt gtagttgact actgcagagt ctccagctgc aaaaatgtct ggattacttg
     4981 tctgcatgta ttcattcact ttgattgccc cgtttggcag tgtatctact tttccttcga
     5041 tcagttttgt atttggacgg aagcccacac ataaaatgac catatctgta tcaaatgtat
     5101 ctgttggtgt agctacttgt ttcacttttc cttcttcatc tgtgataaat tcagtaacat
     5161 tttcgcctag cgcaagagta acaccgcggt ctgtcaattc tttttctaag atatctgtga
     5221 atggtttatc tagatattta ttcaagatgc gatctaatcc atcaatcaat gtcacttgtt
     5281 ttccagattc gacaaaagct tctactaatt caataccgat atatccacca ccaacaatta
     5341 ctacacgttt tgcttctttc gcttgtgcaa taatttcatt tgcttgattg tagtttttgc
     5401 ataggagtac atttttcgca tcgattccac taatcggtgg gataataggc catgaaccag
     5461 ttgtcatcac taatttatca taagaaaccg tttctgtttc tcctgttttc agatcttttg
     5521 ccgttactgt tttattttct gtatcgacgt ctgttacatc gtgttccatt ttaactgttg
     5581 ctcctaatga agataattct tcaggatttg agtagaacaa gcctgctggg tcctttacta
     5641 ctccgccgac ataaagtgcg ataccgcaag ataagaaaga aatattgtca tttctttcat
     5701 atactgtcac ttctgcttct ggatgatttt ttaaaatact ttttacagct gcagtacctg
     5761 catgagtaca gccaattact actactttca tttatggtcc ctccgattaa cattttgtcc
     5821 ttcgacaaaa ctactataac agaaataaaa cggatacaac agtttaatgc ttgtgaattc
     5881 acaaaggttt gtttgtagtt tcattccttt tcaagcataa gaacccaata tctacaagat
     5941 tcttatttta ataatattca cttttatatc taagattgtg aataaaagac aaaaaaataa
     6001 aaaagctagc gtttttttta cacagctagc tttttttagg tataacactt gtagaaactg
     6061 tttttacatt tgcgcctttt ttgctgactc tttacaatcc ggacagagtc catacaattc
     6121 caaccgatgt tcattaatct caaaatcagt cagtctgctt gcagccattt ctatatcttc
     6181 aagccctggg taatgaaaat caacaatctt accgcagttt tcacaaatta catgataatg
     6241 acgctttgaa ctgaaatcaa atcggctaga agcatcgccg tacttcattt cttgaacaaa
     6301 tccaatcgaa gtaaataagc gaaggttatt gtaaacagtc gcaacactca tatttggaaa
     6361 ccgatgttca agtactttat aaatctcatc agccgttgga tgggaatgat gttcaatcaa
     6421 atattctaaa accgcgtacc gttgaggtgt gattcgaatg ttctcttttt ttaacttttc
     6481 gataacctct tcgactaact tattttccat gtaaacaccc cacgttgtta ttctcgtctt
     6541 atcatactat ttttacattt taaaatccac ttaacatgaa aagaaaattc aattttattg
     6601 tattttaatg aaaaaagacg taaaacagtc gcgttcacta ttctacgtct ctattcaaat
     6661 aatttttctc atcataaatt tttgtgtgtt cttttcatct ttaaaagttt tttacataaa
     6721 cctatctgga gaaaaaccaa gtgccttata tctcttggta aagggctgta gataaataac
     6781 gttcaccatt gtcgggaacc acagctaata ccttcttgcc ttttcctaat tcttttgcga
     6841 cttttagcgc ggcagcaatt gcagcgccag atgagatgcc tactaaaatc ccttcttttc
     6901 gcccaacttc tctagctgtt tccatcgctt cgtctccact aatagaaagg actttatcat
     6961 acacagaagt atccagtgtt ttaggaacaa aacctgtacc gattccttgg attttgtgag
     7021 gtcccgcttc tttcccttct aaaatagcag attctgctgg ttctaccccg attaattcga
     7081 ttttcggata cacacgtttt aattctcggc cagcgccagt gatcgttcct cctgtaccga
     7141 tacctgcaac aaacgcgtcc aagccattta ctccaaatgc ttgatgtatc tcaggaccag
     7201 tcgtcttctc atgcacaaga ggatttgctt cattttcaaa ttgtaatggt aaaaagtagc
     7261 cgttttcttt tgccaaacgt tctgcttctc tgattgatcc agaaatacca tccgcaccag
     7321 gagtcaaaat caactctgcg ccgtaccctt tcatcaataa acgtctttcg atactcatcg
     7381 tctcaggcat cacgatgatt actttatatc cttttgcaac tcctaccatc gacaatccga
     7441 ttcctgtatt tccagaagta ggttcaatga tagtatcacc tggtttcagt agaccatcgt
     7501 gttctgcttt ttctatcata cttaaagcaa tacgatcttt tacacttcct cctggattaa
     7561 aaaactcaag ctttacaaaa acatctgctg aatcttctgg tacgatttta tttaatttga
     7621 cgattggcgt tttgccaatc aattcggtga ctgagttata gatagctgtc atcttcaatt
     7681 cctccttgtc tttgctcttc ttacttatca taatacaatc tgttcattta tggcgagtga
     7741 ttcgtctttg actagcaaaa aaaagacaaa gatataccat atatggtaat ttctttgcct
     7801 ttttatctaa atcaaacgtt tagttcttga ccaactaata aagaataact ggacatagac
     7861 atgccatttt tcgccattag atcatcaaca gacatgccaa atttttgtgc aatatcccat
     7921 agcgtatcac ctgacacgac tgtatatgtc tgaccattat ttgtatttac atctgttgtt
     7981 gaagatgatg acgttgaagt cgtcgtagta gtcgttgttg ttgtacttgt ggttgttgta
     8041 gccgaacctg ttgatggcgt atcaaaacgg gttaaattat aagtattaat caatgagttc
     8101 aatttggccc cataatttgg atcggtagca taacgacctg tcaagtacgc tgttgcatca
     8161 taaaaacttg ttgtattgct tttccacacg ccagcataat gataatcgcc cgtggaaaaa
     8221 ctggttgaac gaagaacatt cgcatggtct tggaaggatt cccaataaga agaatatttt
     8281 cggaatggct cattcatcgt tacccattgt ccgtttacat attctttggt tgccatataa
     8341 acgacctgtc cgttatagct tcctttcaca ccgaatagat tataatttgg cgcactagct
     8401 aatccggaag tgccatatgc actttccaac aacgcttgag cgatcatgac tgaagcatac
     8461 aagtcattac ttgcagcaac ttctgacgca gaccagccga tttgatcgat aaacgcttga
     8521 ggatcagcga ctgtttgaga tccttgttca tctgcatgaa catgttgcgg taccgctgta
     8581 accatcatcg gcccaactaa aagactcgtt ccaattaaag gagcaatcgc ttttttattg
     8641 atcaccattt ttcttttagg tctatggtgc cgggcactgc gggtcttcag ctcttgcatt
     8701 caaattcctc ctcaaaataa aaaaacttaa taaaaagttc tcttcttaaa acaaacttaa
     8761 ttaattaaat tatactttta att
//
LOCUS       DACDIG010000093         8780 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.093, whole genome shotgun sequence.
ACCESSION   DACDIG010000093 DACDIG010000000
VERSION     DACDIG010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.093"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..271
                     /locus_tag="IV247_002508"
     CDS             <1..271
                     /locus_tag="IV247_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8778533.1"
                     /translation="SNWKSISNTNDIDIYFADPGTPSQRGLNEHSNGLLRKDGLPKEM
                     DFNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA"
     gene            complement(224..460)
                     /locus_tag="IV247_002509"
     CDS             complement(224..460)
                     /locus_tag="IV247_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8778534.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFLMILFVKLSETKLSIYVTQKNF"
     gene            complement(880..2001)
                     /locus_tag="IV247_002510"
     CDS             complement(880..2001)
                     /locus_tag="IV247_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAP8778535.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVMDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYKPG"
     gene            2329..3738
                     /locus_tag="IV247_002511"
     CDS             2329..3738
                     /locus_tag="IV247_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAP8778536.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3735..4463
                     /locus_tag="IV247_002512"
     CDS             3735..4463
                     /locus_tag="IV247_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8778537.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4591..5502)
                     /locus_tag="IV247_002513"
     CDS             complement(4591..5502)
                     /locus_tag="IV247_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8778538.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5519..6526)
                     /locus_tag="IV247_002514"
     CDS             complement(5519..6526)
                     /locus_tag="IV247_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP8778539.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6523..8652)
                     /locus_tag="IV247_002515"
     CDS             complement(6523..8652)
                     /locus_tag="IV247_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8778540.1"
                     /translation="MKKKLFRIVGIVFISIVLLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8657..8764)
                     /locus_tag="IV247_002516"
     CDS             complement(8657..8764)
                     /locus_tag="IV247_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778541.1"
                     /translation="MQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2706 a         1784 c         1530 g         2760 t
ORIGIN      
        1 ctctaattgg aaaagtatta gtaatacgaa tgatattgat atttatttcg cagacccagg
       61 aacgccttcc caacggggat taaatgaaca ttcaaacgga ctcctccgaa aagatggact
      121 accaaaagaa atggacttca accaagtcaa tcaaggattc atctcatccg ttgcgtccaa
      181 aagaaaccat atccctagaa aatcactaaa ttaccaaaca ccattagaag tttttttgag
      241 ttacgtaaat ggaaagtttt gtctcgctta atttgacaaa taatatcatt aaaaagttag
      301 taaataattg ccctctaagt tccacggtct gttcattaat aactttatat cctcgttttt
      361 caaaaaatgg ctttgcggta atggaagcgt gggtagtgat tttttgcata ccagtttcta
      421 aaacatggtt ttccaattca tctgcaattt tagtcgcaat accttttcct tgatggctat
      481 gtttttgtca caacttcagt attcctgtaa caatacggat ttcagtatac aacaacagct
      541 tttgaaaaga tacttgcaaa aggattaaga tatctatgac ctgctatgct cactgatgtt
      601 gaaaagaatg ttttattagg attgaggtaa gcaagatagt gaatatgtcc ggaaagcctg
      661 taaaataagg cttttcggac atatgtgaag ttataaaaag atagcgaaaa atctatttag
      721 tatcactaat gttgcgaaca tatcgtcaga atagtctgac atttatttct tgctaaaaat
      781 ttcaaaaaag tcaagtgctg acgcagcgga tgactatttt ccagaaaatt ttagtatgta
      841 ttcaaaaatc aaacgtgaca acagctatta actacatcct catccaggtt tgtaacgttt
      901 gaaccactct tctgcgggct caaacattaa gtaatggaag gtacgtttca attgaaacag
      961 cgtcgctgtc gtattcaact caatttttat cacataagtg agaagcgttg cgattaaggt
     1021 gaggatgact tgatttatcg ccccttgttc actatgtgaa tatagtcgtt taatggtcag
     1081 attttgtttt aagtgtttga acaacaattc aatctgccaa cgggcttgat acatgtcggc
     1141 cacttcgttt ggcgatacat caaagagatt ggtcaccatt cgcaggattt tttgtccctt
     1201 ggtaagaacc gttaccaacc taaaacgctc tgtcacatgg ttgacaccgc cgacaagaat
     1261 cacttgttgg tcactgatta tcgtcccatc acgggttgtt gtatctgcta cttcaatttg
     1321 atccaagatg gtaatttttg tattagattt tgtccgtgta acgaagaaat agccgtcacg
     1381 atttagtttg tctaacaact tgtaatcaaa gtagccgcga tccatcacat acgttgcttg
     1441 cgttttatca acaagaactt caaggtgatt acggtcatgc tcactcgcat ttgtcatcgt
     1501 aaaactttca ggatactgat tggttttatc aagataacag agattcaaat gtagcttgat
     1561 gccagacttt gttttccgaa acttggccca tggaaaccat gctttgttca acggaatagt
     1621 cgttgaatca atcaattgta gactggttgt ttttgagctt ggctgctgag cagaaattct
     1681 ggcgactaat tgcgtaaaaa tttccatcaa tacttcttgc ttgatttctg gcgctctacg
     1741 agataacgag ctgtaacaca gggaatcaat gccaagttcc ttgcagaggc gctgatctaa
     1801 aaaggcgcga tcaatctcgc gatagcttgg caattcttca taaaccgcat gaagtaacac
     1861 tttaagcgtc gtttggaagt ctagtttttt actgtagaaa tcaaagtcgc taattaagct
     1921 ttgagagttt tctgataaac cagaaaaatt tattgcagaa aaccattttt gaaatgaggt
     1981 ttttacgtta tacttatcca tgcgtgagtc ctttttattg gtcttggata agtcatccga
     2041 gaatagtata aaggactttt ttgcgtttgg atagctttgt attaggcttt tgatgggtta
     2101 aaaagtcttt gcaacactag taatttagta tttatgtata aattgaaaga tattttacca
     2161 ccacttagtc ttcttaaaag tgatccaaca ttttgtttgt ggcagaattt aactacacac
     2221 taatgcagcc gaaagtacac aactatgcag cagtttgact gcgaactggt ttccttccat
     2281 cataagtttg aatgacttag tgatggaggt acagaaaggt tgttaacttt ggatgatatt
     2341 aaaacaataa aacatttgag aaataatcgt gatcagtcaa ttgatcagat aagaaaagca
     2401 atgaatatca attggcgaac agcgaaaaaa tacgccgata gtgatcaact attcattaca
     2461 gatcccactc gaaaaaacaa atccatgatg gatgatggtt ggggagaaat catcggtagt
     2521 tggctagaag aagacctacg tttacgcaga aaacttcgta gaacggccaa agctatccat
     2581 gaacaattga aacctttggg tttccctggc tcctatcgaa cagtttgtgt atacatttct
     2641 caatggaaaa agcagcgtta cgacgaaacg aattaccaag ctgtcgatcg tcttgatcat
     2701 ccgatcggag aagcacaggt cgattttgga acaatggaag ttatgcatga agatcacatg
     2761 aaagatatac aacttcttat catgtcattt ccctatagta atgcgacgtc cgcaagtgcc
     2821 atgccagctc aaaaccaaga gtgtttcctg gaagcattaa aacaactatt taatcaatgc
     2881 ggcggcgttc caaaaacgat cagaatagat aacttaactc cagcggtgaa aaaaacaaaa
     2941 tcaaaatttg aagaagcaca actaaccgat gaattcttac gtttcaccat tcattatggt
     3001 tgtgatgttc aagtctgtaa cccacgtagt gggcatgaaa aaggacatgt cgaaaataaa
     3061 gtgggttatg tccgatacaa tttcttctcg acttcgccaa tcatggactc ttttgagtcc
     3121 ctaaataagc aacttgaaga acaattaaaa gcagatcgaa aaaggacgca ttattcaaaa
     3181 catcaattaa tcgaagattt gtggaagaaa gaaaataaag cgttacattc tcttccacaa
     3241 gaggattacc caatttttaa agaagtggaa gctaaactaa atcgttatca cgaaatgaaa
     3301 ttggatcaaa cggttattca tattcctaaa gcatatggct attctcaatt gactgttgtt
     3361 ttgacttgga ataaattcaa agttcttacg cctcatggag aaatattaat gaacgaatat
     3421 cgcccctata tgaacaaaaa aagattgatc ccatggagtg acatcatgaa acaatggagt
     3481 aataaattga atgtcgttag ttattcgagg tattggaagt acttgcctgc acgccttcaa
     3541 aattatctag cgattgataa taatcacctg aaatacagac ggataaaaca attactaagt
     3601 ttattagcga cctacgatat ccaacaaatt aatgaacaat tttacgaatt agtcgttcaa
     3661 gaagacgccc cctctgactt tgatgtgaat tggtcaaact atgatgcatt aacaaaaaca
     3721 ggaggtgtgc aaaaatgatt ggacagacgt tagaagatta ttgtaaaaag cttcgattaa
     3781 gccatatcat cacgaacata ccaagagctt ctttcgaagc tccagaacaa tttattaccg
     3841 atttattccg tttagaactg gagaccagac agcagatcaa attagaacgg atggaaaaat
     3901 tagccaaatt tcctgtacca aaatcgctaa agaactatga atggcacgaa gatatttttc
     3961 taccatctca tctagataag gagacccttg tctctcttga ttttctaaaa aggcaagaaa
     4021 acattgtttg tgttggctct cctggtacag ggaaaacaca tctagcgatc gcattaggga
     4081 gaaaagcctg tagtgaaggt gtttcaacca aatttttccg agttaacaat ctcgtagagc
     4141 aattagagaa tgcattaaaa aaacaacagc tagccagttt caagaagcga ttcgaaacaa
     4201 cagaattaat tattcttgat gaaatggggt acttaccatt gacaaaagaa ggagctgaac
     4261 ttctcttcca actgatttca gattggtatg agcaaaagag cattattatt acctctaatt
     4321 tagaattcag tcaatggaat cgagtgttta tggattctcg attaacagct gctctcgttg
     4381 atcggctaat ccatcacgct cacattatca gcttcaaagg cgaaagtttc agattgaaaa
     4441 attcattatc taaatcatat tgaaggaaaa aactagttgc agcaaaactc tgcactttcg
     4501 gctgcatttt tttgtaaaaa gctattgcac aacacaaaca tttgaacgac gtaattccaa
     4561 atgttggatt gcttttagat aatcttttat ttattcacaa ttgctaatag tccaattttc
     4621 atttttgtat agttttaatc tatactgaaa aacacttgtt gttttggaca actcatcaag
     4681 atactcaaca ccaacaatca cttgaatcgc tttacccttt tgtttgtaaa taggattcac
     4741 taattccaca aatttcaaat tagtattaat tggttgcatc gcgtcttttt caacataatg
     4801 ctctagttct ttttctgagg ctgctggata aagcctgaaa aacgtctcta aaaactcggt
     4861 gatttcttcc gttgtctttg aatcaatttt tgaatcactt tttacttgtc ttactttatg
     4921 ttttgccttt tctggcttac caatcatagt gggtaaactc gttacaatat gattgccttt
     4981 ttcatcttca aagatcatca gacgatatgc agaagaaatt gattgtgtct ttttcccttc
     5041 tgtaatcttt tgatctacca caaatgaaac gacaaattct tccgagtgtt tttcaacatc
     5101 tagaattttt actgatacaa cctcggaact agtgggaata tccgctcgaa ccatatcttg
     5161 gctcaaagcg agcccttctt ctgtaaggta ctgctcaaga ttagtcattc tcttttcaat
     5221 tacctccttg ttattcttcc atgaaaaata ttctttcaca aaatctacag taaaattttc
     5281 tactccagag gtattcacta ttttctcttt cacgaccact ttctcatgag ttgtatgctg
     5341 atcaatggcc gtgaagtttt tgtagattcc aaaagcaagg ctacaagaca ataaaagcca
     5401 aagggcaagc accatttttc gatgcttgcc cacggatact actcgttctt tcttcttttt
     5461 tggaatcttt tccttgttac ttcgctcaat ttttatcttc attttccatt cctcttttct
     5521 attgtttata ccgaccagca ccaataatgt gctgttgcca atagctatcg gtaagattgg
     5581 tccaacccac cggatctcct gcatgaaaca ttctgttctc tcctgcataa atgcccacat
     5641 gagtcacata agtaccggca tcataagttg aatgaaagaa aatcaaatcg cctggtttgg
     5701 catcttttaa gtcaatatga gtcatagcat catattgatc ctgagccacc cgagggagtt
     5761 tcaaaccaac tttggcataa caccattgag ttaaaccact acaatcaaac ccagttccgg
     5821 gacttgaccc accaaataca taagccgttc cttcatactt gaacgcctcg tcaaaaattc
     5881 cttggactgt tttatcatca aactgtgttg tagttaggta ctgcttcact aataagcaat
     5941 aaaacatatt gccatagtta taccgccacc cactattttc cttaatcgcg atgggattgg
     6001 gataatcgac tttttgacca cccgattttt cttttgaaaa agattcggct aaagcaaaac
     6061 tatgcttctt tccgtttttc gcgacataat ccaaataccc gtcaccatag ttataagcct
     6121 gaatgatact atcttgatcg catcctagtt ctttggaaca cttaaccaaa gacgaaaaat
     6181 agagacacac ttgtttgatg gatgcctcgg tacttaatga attcggtgat aaacctgctg
     6241 attcagaact ttgcatgaca tcttccattg tacctccaga ttcgacttga ataatcgcca
     6301 aaagaatcga aacatactct gttatatctg attctttggc atacttttcc accattgaac
     6361 ggtgtttcaa aactacttct gagacactac tacctccagg agaagttcca gaactaccac
     6421 ttccctcacc gtcctcacca aacaacaaac ccacacacat caagatgccc acaaaagaga
     6481 tggtcaggaa ggttaacgaa ataatactca atttttttaa gttcatcttt tctcacctgg
     6541 ctttggtttt gggcgagtgt tcttttttct ttgtacttgt tgattggttc gctttggaaa
     6601 agtgaatctc tgtttcactt gttgaaagga attgttcttg cttagtggct gagtgcttcc
     6661 gtttggaatg acttcttgac tagaaagttt ccgtttgatt tcaggattcg aatgtacttg
     6721 tacccttggt tttaaagacc tattttctgg tagttcattt ttctgtggtt ttatctcctt
     6781 ttttatcgga acattttccc ttcctttctc atgacgattc catgcttcgt tcaatacttt
     6841 gcgtttttca tccatttttg cgtctcttcg ttgtcgttgt ttttctcgca actcccctcg
     6901 atttgctttt tcttcgaaag gccctcgctt aaagtcctct ggtacttttt tcgtcttttc
     6961 catgccttta tgaaggctgt atttcaagtt agttggtgca tcggttactt gattttttgc
     7021 ttgtttcgct ttgtccttca cgatttcttt tgtatcgagt acttttccaa cttttctacc
     7081 aactaaattt attcgtgatt ctttcttcgt gcccactggt tgttcttgtt ttttcagatc
     7141 agaatttagc tcgttaatgc ttctcgcgga atggttcttt ctattggaca ctctattgat
     7201 ttttgaacta cctttccctt ttgtagaact ggtagctagt ttcccaccaa ctgctgcacc
     7261 gccaccaaaa gcaaaagttc gacccacatt tcgattaagc tgtcgcaatt ttcgattcat
     7321 caacatttgc ggtttccgca tgactcgcct tcccattgac tgactatcat tactttgaag
     7381 gttaaacatg ctcataatgt cacctaattt gaaatagata ccggcaaagg tcacaatttg
     7441 tagaaaagct accataaaga agggataggt ggccgaaata ttgaaaaaca tcgtggagat
     7501 actgaatgca gtcgtaatga ctaaggtaac tcccgctcgc atcataatcg tattgaacaa
     7561 acgaataatc gcccttttgc ccaaactctc atacgtcggc aacatcgaca ataagaaact
     7621 aatgggcaga aacatcgcaa agataatgaa aaggatttgt gagaacaaca taatccctgt
     7681 cagaagaaac acaaaaaagg aaatcccaat attaaacaac actaaaaaga ccacggttcc
     7741 taaacggttc atggtcttgg taatactgag attcgcattg tcattatctt caatctctgc
     7801 tttgactgct tcttcccgat ccttaccctt attttcatcc ggactcacgg ataaaatctt
     7861 attaacccga ccctcgccaa tttcttctat attggaatca tcgaattgta atagtaacca
     7921 gggttgttga acttgaatcg aaaataaact gtctcgaatc aaatccacac tatcttttcc
     7981 ctggctttct gaattaggta caacaatctt ggttccaagg ctaagagccg cttcactaac
     8041 gtctgagcta aagtcattga tttttgtaat ataggtgggc gcatacgcaa tgaaagaacc
     8101 cgagagtaga aaaatcatca caaaattaat gacggttcgg actgctttgg tagtttctcg
     8161 ttttaataat ccggtatacg ccacataaac tcctagagct aaaatcatca ataataggaa
     8221 cccaaagtag aatcccgaag tctgtaatcc attttcagtg ataccggcta aagtttggat
     8281 attctttcca atacttttag ccgtatccga aatgaaatcc aacttgtaag cttcttgaac
     8341 cacatagccc gtggcgtttg ataaatacag acttactgtc caaataaaat tggtaatggc
     8401 ataaagacca tacatcacgc ttttccctaa gccatccccc caattccaag gtaaccaatc
     8461 ccaagaacta tccacaaaga aatccagttg ataattgttc agagaatatt tggaataaag
     8521 gtttccggct tcaactgtat catcaaccag acctgtcgcc tcagctaccg taccgactaa
     8581 actcaataga actgtcaata ggacgatgct aataaaaacg attcccacaa tccgaaaaag
     8641 tttctttttc atcccctcac tccattctct cttgaaccgg cggacgagtg tcaaaagcat
     8701 ggaagagttc ttcaaagact ggatgaactt gaaccacacc tactcgccca tacaagtctt
     8761 gcatcaagca ttgaccgttt
//
LOCUS       DACDIG010000094         8634 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.094, whole genome shotgun sequence.
ACCESSION   DACDIG010000094 DACDIG010000000
VERSION     DACDIG010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.094"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            156..905
                     /locus_tag="IV247_002517"
     CDS             156..905
                     /locus_tag="IV247_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAP8778542.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSRGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKLTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(1089..1256)
                     /locus_tag="IV247_002518"
     CDS             complement(1089..1256)
                     /locus_tag="IV247_002518"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778543.1"
                     /translation="MKDIQSGNVQETIDKWKGKHYRFTEAQKQALEYYQKLEESLRFN
                     DEARKYLKELL"
     gene            complement(1272..2366)
                     /locus_tag="IV247_002519"
     CDS             complement(1272..2366)
                     /locus_tag="IV247_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778544.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSK"
     gene            complement(2445..3089)
                     /gene="pcp"
                     /locus_tag="IV247_002520"
     CDS             complement(2445..3089)
                     /gene="pcp"
                     /locus_tag="IV247_002520"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAP8778545.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(3288..4232)
                     /locus_tag="IV247_002521"
     CDS             complement(3288..4232)
                     /locus_tag="IV247_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAP8778546.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(4234..4920)
                     /locus_tag="IV247_002522"
     CDS             complement(4234..4920)
                     /locus_tag="IV247_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAP8778547.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            5393..5713
                     /locus_tag="IV247_002523"
     CDS             5393..5713
                     /locus_tag="IV247_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAP8778548.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGLLLISIIGIKI
                     TSGH"
     gene            5874..6779
                     /locus_tag="IV247_002524"
     CDS             5874..6779
                     /locus_tag="IV247_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8778549.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSEGTIRIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVKQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDQAARTILTDAGYPLEDPDKIKLLKKNEELLGTVIQLLSAENVHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            6776..7549
                     /locus_tag="IV247_002525"
     CDS             6776..7549
                     /locus_tag="IV247_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8778550.1"
                     /translation="MTLLIKQELFKLIKKKSTAVLSVLLVALLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMESQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSIYLYLLAIIVTVLMKLILFPSISFTEKISTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIALFLALGYLVFKRKNV"
     gene            complement(7726..>8634)
                     /locus_tag="IV247_002526"
     CDS             complement(7726..>8634)
                     /locus_tag="IV247_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8778551.1"
                     /translation="AISTFSIQQFINNFSSDIILNANGQPIPFSSQVLSCIMVMLWVF
                     VGIEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPA
                     MGYVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNK
                     NGAPTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQR
                     KEWVQFLVGFLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHL
                     DKILLIGVTMVGVIAMVLFATGMLHV"
BASE COUNT         2598 a         1753 c         1542 g         2741 t
ORIGIN      
        1 taagaaatta tattttcaag aaaacaaaca gaaaattatc ataacacaaa ttatttataa
       61 ttgaaagact agtttttttt caagaaaagc atacaataga ttgtgttagt aaatacagaa
      121 cacactacat ttagtgtctt aaggagtcct catacatgaa gtcatatttt cagtttttag
      181 ctcatcagct aaaaggctgg ccgcaacaaa attattatct tttctttttc agtcgtggct
      241 gccaggtcat gactcttgtc aatagtccca tcacttggct gtccgttatt acatttatcg
      301 gtaccacgct tggtgtattg tgtattttat ccatcaatgc ggcaaaatca gtcaacggta
      361 ttttaggtat tttgtctgcg atttgtttta ttatcgttgg gttttcagca aagaactatt
      421 taagtatcgg tgaacagatt gcttatatga tcacattgga tatccccgta ttgttaagta
      481 aagaatggaa tcataatatg gcttcaaaaa tccgaaaatt aactggaaaa agctggttta
      541 tcgcaatcat cagtactttc cttgtctatc ttgtttcagg attcctgatt ggtcgttata
      601 cagatgatcc tcggccgtgg gtagatgcca ttgctttttc tattagcttg actgctggta
      661 tcatcagttt cttgcgttac aacaaccaat attactggtg gttagcttca gggcttgctc
      721 aaatggtcct ttggtatatt tcatttacgc atggatctgc aagtttggcg atgttcgtga
      781 acagctctgt ttacttgctg aatgatgtcc ttgcatttac cgtttcacct tggtataacc
      841 aaaaagaacg tgaacgtatg caaaaaatgg aagaagaata ctacaacgca caacaacacg
      901 cataaaaaaa ctgagcgtgg gacaaaagta aagaacactt ttgtctcacg ctctaaacac
      961 gtataaacgg cggaatcaga agcaactcct tcggaaataa gctgaaattc acaaaaattt
     1021 gaaaagcaat tttcgtgaat ttcttcttat ttcttggagt taaacgcttc tgtcccagcc
     1081 tctatttttt atagtaattc cttcagatac tttcttgcct catcattgaa acgcagtgat
     1141 tcttctaact tctgataata ttccaacgct tgtttctgag cttcagtaaa acgataatgt
     1201 tttcctttcc atttgtcgat tgtctcttgc acatttccag attggatatc tttcactact
     1261 agttgccatt tttattttga gactgtacgg tctttcttat cagcagattg tatagcttct
     1321 cgttctttac gcaatacttt taaaaaacca tttctgacta tccgaggaat gctgtcgaag
     1381 aacccttctg taaaaccata gcgacaatat tgcttccaaa taaatagata gatttttggt
     1441 tggatgaatc gttggttttt cgcaaaatat tccatctgag cagtgacttc tggtgttcgt
     1501 tgacgttttg tctttctcaa cacggaaata aatgtttcaa aataagcatt aggatgtact
     1561 ggcacttctt catatacgat ggattgccaa tctttacgta aaaatgcgcg atagttgatc
     1621 aagaattctt ctacatcctt tactaattct tctgcaaagt cgtcaaatag ttcttttttt
     1681 atctgtcgct tttttgactg cccattgatg tgttcactta ttttcttgat ttcattttcc
     1741 aatgcttgat aatccgctat gtaaatcggc atttctactt ggcgaaattc tgcgagccgc
     1801 tgagctaatt ttatatccgg taaatatggc tgtttcatcc gacgattttg gtacaagaca
     1861 aaacagtatt tttgccatag tccttcagga aaatcgatat tcttctgctt aagccataat
     1921 aattcgtcta aggccagatc aacttttgtc attccctgat ggatcgaagc tgccactgtc
     1981 ggagagacat gcaggtgatc ggcaaagtgg ttgatgccta gttttaggat ctttccggtt
     2041 tttggcgatt taacgatgta ttgcactttt aattctctac cacactcaca atacaatttt
     2101 tgctggcttc gttttgttcg aaaaaacgga ttgatcttga agtcagaaaa cacccaatct
     2161 gttgcagcta aataatgttc ttccataaaa cgagagctga ttagatatcg gcggtgctta
     2221 tcgactagtt cccgctgttt tttcgttaat tttgcataga tctcccaacg ttgactgctt
     2281 gtgtattgtt tagaaatttt gatgggatag cttttgctga tttcttttgg aaaccattct
     2341 tcaaagacgg attgccattc ttccacggct gtttctttcc tttctaatta caaaaagctg
     2401 gctactaaca taacgttagc aaccagccag tgtttcttgc taaattagtg agttgttccg
     2461 gcatttccgg aaatatcttc tttattttct actgctgctt caactgcata agtcaaagaa
     2521 gaagcaatgt cttctaaaga catagaaggc gtacccactg gacgatcgat cacttgatcg
     2581 ggtgagaatg gcacgtggat gaacccacct cgaattgccg ggaattgtgt ttcgatcaaa
     2641 tgaagcaaac gatacatgat atcattgcag acaaatgttc ctgctgtata agagatgtac
     2701 gctggtagac catgatcttg tacatttttt gtcattgctt tgattggcaa tgaggtaaag
     2761 taagcggctg gaccgttttc ttctaaagcc gtatcgaatg gctgatttcc ttcattatca
     2821 gggattcttg cttctgctag attgattgcg acacgttcga acgagactgc tgaccgccca
     2881 cctgcttgtc cgacacagat cacgacatct ggttggtgct gttggatggc ttctttgact
     2941 acttgactgc ttttttcgaa tacggtcggt acttccactt tgatgatttc agcaccgctg
     3001 atttcatctg gcattttttt tactgcttca taggctggat tcactttgtc cccaccaaat
     3061 ggatcaaaac ctgttactag tactttcatc tgttgttcct ccgtccgatt gatttaacat
     3121 atggttatct tttatcatac cattttcctt gaacagcgta aataaaggaa acaatagttt
     3181 aaataacata acagatcaaa cttaatatat tcttataaaa aaatagtgtt ccactacaat
     3241 gttagaagag gttgcgacaa ttattctgtc acaacccctc tcttctatta tttaaataga
     3301 atcatataag caatttggaa aactagcata aataacgcaa taaacaactg gtttttgatc
     3361 actccgtatt tgtctttcat ctccagcatc gctacaggca cgatattgaa gttagcagcc
     3421 atcggtgtca gcagggtccc acaaaaaccg caagtcaatg caaccattcc tattaacgct
     3481 ggattcgctc catgactaaa tacgaatggt gcaccgatcc caaccgtcat cacggtgatg
     3541 gccgcaaatg cattccccat gattaccgta aatatcacca tgccaagagc gtaaatgatg
     3601 atgccgatgt tcacatttcc ttctggaacg atcgtaccta ctgcattgga aatcactgtc
     3661 ccaactccag cgcttgtaaa taccgcacct agagaagcta aaagcattgg caacatgctt
     3721 aacggcccta ctgtgcccag catatctgct gcatcatcta aaaatgtact tggtcgatta
     3781 tctcgagaaa agaacatcaa gattagcatg gctgcaaaaa caccgacgcc cacgccgact
     3841 aaagcaccta aattggtaaa tagagcaaac aagatcgcaa agacaccgat actcaaagct
     3901 ggaataaaaa ttttcattcc tagtttgtct gacatttttt ccatatattc ttttgatgga
     3961 agccggcttt ctcctttgct gactttctta gcaattgctg gtatggtcat cgcaaagatc
     4021 aagccaccgt tcacgattgc cggaagccat cttcctcctg caataactac ccctaagacc
     4081 gtccagaaaa aggctgaccc ataacgatga ggattggttt tatctgttaa atttcttact
     4141 ccagtgtaaa tcaatacgag tcccatcacg atataaagga tctccagtaa tttatcgcct
     4201 aataaaatat caggatttgt aaaaaagtcc atcttattgc ccctttcctg gataatattt
     4261 cttcgctaat ttcttttctt taagatagaa gtagacactt gccacaacta aagccgtgat
     4321 agccactgga acttccacta aagcaagctg tcccaatgtt acgtcatatc ctagatcttt
     4381 cagtgttgat tggactagca acgcccctgc tcctccgatg aatagtactt ggccaaagaa
     4441 ccagcagaca ttttccatag ctgaagccat accttttagt tcttcttcat gcttaggatg
     4501 gacttctaac ccttttgatt cgatggtccc caatgccatc ggtaaaatga ttggacgaac
     4561 aaatccagca actccaccaa aactcacgtt aaaagccgca aagatcattc gaaagatccc
     4621 ataaatatct acaatcatac cagcagatac tttcttgaat cgcttgatca aggaagccgc
     4681 tgattctttc agaccatttc tttcaagcgt tcctgttgcc aacataatga tgataaaaat
     4741 cgccatcccg cgattgttta caaaacttgt acctaaaagt tctaacattc cttgaatgcc
     4801 aagaccgcca gtcaaagctg taacaactaa agcaaccatt acgatcaata tcgagtctaa
     4861 tttaaatgca aacccgagaa taacaatcaa gacgcctaaa aggctaagat aattttccat
     4921 atattttcct ccataaaatg acaatatttt tttaattata aagcaaaaca cataaaaaat
     4981 atagttgttt tctcacgaaa tagacactat ttttaaatat ttttcgttat ttttttatta
     5041 tataattcac cttacttatt gtaagataaa gcttttttta atttattgcc tgctaatata
     5101 taaaaataag gtctagttac tgttttttta aatgattaat aaataacaac tcctttctat
     5161 gaaattgcaa agcattcatc ttcttttctg ctaacgatat atccgtcaat gcttgttaca
     5221 ctttactaaa aagtatgaga ttgccgatat ctggataggt gtgctatact acagaaaaag
     5281 atatacgatc tgccaccggg tagtaaaaaa gaggatgatc attccattag gtctttgaag
     5341 ggatgattct cctctttttg tgtatttttt atattgaagg aggaacgtat gtatggcatg
     5401 gatggaatta atcatagcag gaattttgga agtattctgg tcaacgatga tgaaatggag
     5461 tgatggtttt tctaaaatca attattcttt ttacacagtg attggaatga ttgctagttt
     5521 ttactttttg tcaaaggcaa tcaaaagttt gcctatgagt ctctcttatc ctatttggac
     5581 agggattggc gcagttggtt cggtcattat tggtgtggtc ttgtttcatg ataagctgaa
     5641 tctttccact tggttctttg tcgggttgct gctcatcagc atcattggca tcaaaatcac
     5701 tagcggtcac tagatactta gacaagcaga caaggactga tcagaaaaag cggggctttt
     5761 tatttgatat tcctctttct atgaaaaaac aagaaaataa gactaatgac ctagcgactt
     5821 ctctactcat caggtgtatg cttgtactat ctatagaaaa ggagtgaata tttttgaaaa
     5881 gtctttcggt ccaagaatta tccaagaact ttggtcacaa acaagccctc agcgatgtca
     5941 gttttagctt atctcctggc aaaatcgttg gattagtagg tcctaatggc gctggaaaaa
     6001 caacgatcat gaaagctatt ttaggactga tccattactc agaaggaacg atccgtattg
     6061 gtgaaaatga gatttctccc tctagtcata aaggattaga acaagtagga gcattgatcg
     6121 aatatccggg tatctatcca ttcttaacag gttatgatca tctgaagtta ttctctgaaa
     6181 caaacgatgt atctgctatc gacactatcg tgaaacagct caagatggaa aaatacattc
     6241 acaaaaaagc gaaatcctac tcgctgggaa tgaaacaaaa attgggaatt gctcttgcct
     6301 tactgaacca tccgtctatc gttattttag atgagccgat gaacggtttg gatccacaag
     6361 caacaagaga tgtgcgaaaa atcattaccg acttggctac tcagggcacc tctttcttga
     6421 tctccagtca cttactcagc gagcttgaaa aaatggtcga tgagctgatc ctgatcaatc
     6481 aaggaaaaat catcaaacaa tgtacaatgg ctgaattgaa ccaaacagcg caagatttca
     6541 tcgttatctc tacgacaaat gatcaagctg ctcgaacgat cttgacagac gctggatatc
     6601 ccttggaaga tcctgataaa atcaagcttc taaaaaaaaa cgaagagtta ttaggaactg
     6661 ttatccagtt attgtctgca gaaaatgtcc atgtgacaga tgtccagcat ctgacgaatg
     6721 atttggaatc ttccctattg gaaattctgg attcagtaaa gggggaagaa gaaaaatgac
     6781 gttgcttata aaacaagaat tatttaaatt gatcaagaaa aagtcaactg ctgttttatc
     6841 tgttctatta gttgctttgc tcataggaac agcacttcta gccaaaaaat acacgacgat
     6901 cattgatcca gttgaaatga ctgctcagct tttcagtgcc acatcttgga ttgtctttat
     6961 catgattgct gctgcaagta cgatcatttc aatggaatca caatatggta cattgaaaaa
     7021 tctcctttat cgaaagtatt ctcgaggaga aatattagta agtaaatgga tcacattagt
     7081 tatttattct atttatttat accttttagc tattattgtc accgtgctga tgaaactgat
     7141 ccttttccct tcgatttcct ttacggagaa gatttctact ggtcagacac tgatccaaag
     7201 cttattcact tatactttgg gtagttatat cggtttatgg ctaattttaa gtttagtgtt
     7261 gatgattgct tgttttatca atagttccgg tgcctccatc tcagcaggga tcgtctttta
     7321 ttttgcttca tcaatcatat ctggtatcct catcgcactc attcaaaaat gggagtggat
     7381 caaatggaat ccaatcagta tgctgaatct gcaaaaccaa attggcaacg aagagattat
     7441 gaagcaattg actcatcttt ctactaatca aatgctgttt ggcaatttag cttacattgc
     7501 tttgttctta gcattgggat acctcgtatt caagcgaaaa aatgtatgat ggttcattaa
     7561 caaagaagtt gtgacagaag tggccagttt caagaaattt cggcttattt ttagaagaag
     7621 ctacttcttg aacaccattt attcaaaaaa agaagcgatg acacgaatgt tgtcaccgct
     7681 tcttttttga ataaaccaca ggcttcacag cctctcttta tacttctata cgtgaagcat
     7741 cccagtggca aataaaacca ttgcgattac tccgaccatc gtcacaccga taagtaaaat
     7801 cttatctaag tggatatgtt gtacaggttc gttttgttct ttacgtgctt tgaggtaaaa
     7861 ataaatacct ggtaagaaaa gaacaagaac aagcatcact tcattaaagg ctacaactag
     7921 catggcccat acttgaaaag cacttgctaa aaaccctact aaaaattgga cccactcttt
     7981 ccgctgataa gaaagcttca tttggtacag ccctaagaaa agccacgtga agaaaatcgc
     8041 tgctgtacca agtgtataag caaagttata tgcactgtca gtgaataata atgaaagtaa
     8101 aaagacattc gtacataacg tcgtcaatac gatagcataa gttggtgccc catttttgtt
     8161 gagttttccc catgctttag gcaaaagtcc gtcctcagcc atattcccca ttgtttcaat
     8221 cggcaagatt gtccatgaga gccaaacccc aactaaagaa atgatcaaac cgccgttgat
     8281 cacgaccgcc ccccatttac caaccatcat ttcaaataca tatcccatag caggttgcgc
     8341 tttgatcgtt tgcagctgat tcatcgtcat tgtgccatat ggaagcatgg aaaccaagac
     8401 gtaaataatg atcaaaccga atattcctag aatcgttgct ttcccaacat ccgattgttt
     8461 ttttgctcgt gaacctacga cagaagctcc ttcaatacca acgaataccc agagcatcac
     8521 catgatgcaa ctcaatactt gactagaaaa cgggatcggc tgcccattcg cattcaatat
     8581 gatatcgcta gaaaagttat taatgaattg ttggatcgaa aaggtgctga tagc
//
LOCUS       DACDIG010000095         8582 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.095, whole genome shotgun sequence.
ACCESSION   DACDIG010000095 DACDIG010000000
VERSION     DACDIG010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.095"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..749
                     /locus_tag="IV247_002527"
     CDS             <1..749
                     /locus_tag="IV247_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8778552.1"
                     /translation="GVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYT
                     DSLEKDQEKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYR
                     YLKNIKNEYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAI
                     EASIESQDRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSS
                     IEEINKKNPNMFVNYQNDPYQFTTQVILLF"
     gene            764..1516
                     /locus_tag="IV247_002528"
     CDS             764..1516
                     /locus_tag="IV247_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8778553.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(2219..2434)
                     /locus_tag="IV247_002529"
     CDS             complement(2219..2434)
                     /locus_tag="IV247_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAP8778554.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(2453..2689)
                     /locus_tag="IV247_002530"
     CDS             complement(2453..2689)
                     /locus_tag="IV247_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAP8778555.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            2947..5100
                     /locus_tag="IV247_002531"
     CDS             2947..5100
                     /locus_tag="IV247_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HAP8778556.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            5069..6484
                     /locus_tag="IV247_002532"
     CDS             5069..6484
                     /locus_tag="IV247_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAP8778557.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            6517..7551
                     /gene="sppA"
                     /locus_tag="IV247_002533"
     CDS             6517..7551
                     /gene="sppA"
                     /locus_tag="IV247_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP8778558.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            7553..8074
                     /locus_tag="IV247_002534"
     CDS             7553..8074
                     /locus_tag="IV247_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP8778559.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            8071..8457
                     /locus_tag="IV247_002535"
     CDS             8071..8457
                     /locus_tag="IV247_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAP8778560.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
BASE COUNT         3079 a         1223 c         1529 g         2751 t
ORIGIN      
        1 ttggtgtctt aatattttta atcattcaaa tgtttgttgt tctattttta tttattagaa
       61 taactattag gcaacgagaa caatatgaac agcaattgaa aagaaaagaa ttggtttatc
      121 taaaaaaata tactgattca ctggaaaaag atcaagaaaa atttgccaga tttagacatg
      181 actataaaaa tttattgctg agtttaaaag aaatcgctaa tcaaaaccat gattcgatat
      241 tggaaaaaga aataaaagaa ttagagatct attctaattc ttatctaacc aataagtttg
      301 aatatcgtta tttaaaaaat ataaaaaatg aatatttgaa aagtttgttg atagcgaagc
      361 tataccaagc aaataacaat aagatttgtt gtaaattcga atgtccaagt actattattg
      421 aagttcctgt tccaatattt gactgtatta gggtgcttgg aattattctc gataatgcaa
      481 ttgaagcatc tatagagagt caagatagag aactgtctct agctatttac cgagatgata
      541 aacagataga aatttcgata tcgaatagtt gtagagagat actactctct atagatactc
      601 taatgaaaaa aggaataaca acaaaaaagg gacatcaagg gttaggttta tctagtatag
      661 aggaaatcaa taaaaagaat cccaatatgt ttgtgaacta tcaaaacgat ccgtatcaat
      721 ttactactca agttattttg ttgttttgag ggaaggagga tttatgagtt atccaataat
      781 attatgtgaa gatcaaatta tacaattaaa tcagttagaa cgaattattg ataatttcat
      841 tctttttcac gataaagtat ttaagatagt attgaaaaca caaagtccgt tagaagttaa
      901 aaaatacctc aaacaattcc gaccaaagca aggaatatat ttcttagata ttgatttaaa
      961 tcatgaagtt aacggtatag aattagcaga agtaatcaga aaatatgatg ttcaagcaaa
     1021 aatcattttt acaactactc atgatgagat gttacccgta acaataaaaa gaagagttga
     1081 aacgttagga tttgtaacaa aagatcaaac actagatgag tatcgaaacg agattgttga
     1141 gttattgtta ttagcgcaag aaaggataga tgcaacaaaa caggttacga atcaagcatt
     1201 tgttttttcc ataggttctc aaacatttac ttttgatata aatgacattt attttgtaga
     1261 agcttcaaaa ttaccacata gattatcatt gtgtactaaa gatgggcagt acgaatttta
     1321 tggaagaatt agtgagcttg aaaagaaata tcctatgtta acaagaatta gtcgagcgtg
     1381 tttagtaaat atctttaacg ttaaagaaat agattttaaa aaacgttcac tatattttga
     1441 ttcagagttg gccagaaact ttactttagg gaaagcacaa aaaattaagg agaagttgaa
     1501 agaaagcact gtttagtggt tttttcttta gggagattgc atttttctaa taaaatagat
     1561 tccttcacta gtgcaaattt ttctgaatcc tattaaagtt gtaccaattt ttatagctgt
     1621 tattagttac gtgcataaca gcaatattag ttactggagg aatatttcta tggtctggaa
     1681 ccaaaaaagg gccaaatgaa tagttctttt cttgttttta ttaagtacca taaaggggaa
     1741 ttaaaattaa atgtatggtg gaagatgcga aagatttggc aaattctact aatttttaga
     1801 aagtgttttt cattttttag ttaaaaaatt tgtcctgtca agtgcaatcc tatgattgta
     1861 cttgatagga tttgagataa ttactttcct gtttttataa atttaacttg caggaacagg
     1921 ctttaatgca catccgacaa gtacattacc aatacctcct gtaacggccg aaataggtcc
     1981 tcctgcagca ccaactacag cgctgcaagt ccttttgtaa aaatatctct accattacat
     2041 ttccaaagaa tttttatctg actttaataa aagataaaaa tttaatataa ttataaaaac
     2101 tagcacaaga agctttagta aaaagttctg tccattccaa acttttgaaa agataaagaa
     2161 catacctatg ttcgtaatcg acacaaacca atcttgaggt ttgatatttt ttaattgttt
     2221 acgcaccggt ccacatgctg aatccagcac ctaataaagc tccacctaca ccacaagcag
     2281 ctattgcata aggtcctccg gctgaacaaa gttttactcc tgctgttgcc ccagaaaaag
     2341 tagtgcctaa aactcccacg gcactcccac cttgaatatt tttcaactct tctgaagata
     2401 atataaatgt tgtttttgtg tttttaatat gcatactact tattctcctt ttttattttt
     2461 ttcgagcatc ccagccagct gcagcacctc cagcgacagc tccatataac cctccaacaa
     2521 caccgcaagc tggtccgcaa attgctgttc ctaaacctgc accacctact gcccctgtgg
     2581 taagtccacc tacaacttgc cccaattttc cccaagcatt attgcctcca ccaacagttt
     2641 gtgcatttcc acaatactta gttcctttac attttgcatg tttaaacacg cttctttaaa
     2701 taatattttt tacggtgcta ctgtaacata cgtatttgat taacagtaaa aatcttcctg
     2761 aaacttatca agtttattct tgaatagtaa aatttccaaa ataaatttga ttcaggaatg
     2821 aaaaagttag tgttcaagaa ctgttttcaa agtttttgtg gaattatgag acaatatttt
     2881 cactgtatta aagctatatt gagaattttt ctataaaaat tttttagaag gagttgtgtt
     2941 agataagtga taaaatcatt tccttttgtg cagcaacaag atgaaaaaga ttgtggtgtt
     3001 gcttgccttt cgatgatttt aaaatatttt aaaactgata tcccgattca taaattacgg
     3061 gacctatctg ggactgattt agagggtact tcagcatttg gattgaaaaa gacatttgaa
     3121 aaattgtctt ttgattgtat ggctattcaa gcagatagtg agatctggaa agaaagagat
     3181 cttatcttac cattaattgc acatatattg gttgataaca gctatatgca ctacgttgta
     3241 atttataaaa cgaagggaaa cgtactatgg attgcagatc ccgctaatgg aaaaaataga
     3301 aaaacgattg aagcgtttca aaaagaatgg acaggtattc ttttattacc tatgcctaga
     3361 gaaacttata ttcctattaa agaaaaagta gctggattaa gttctttttt gcctataatt
     3421 tggaaacaaa aaggattagt attccacatc gttttagcat cgattttcat tacgtttttt
     3481 ggcataggaa gctcatatta ttttcaaggt attcttgatt ttttcatccc taatcaagca
     3541 cgttcaactt taaatataat ttccattggc ttgatcgctg tctatttatt tcgggtattg
     3601 tttgaatata gtcgtagtta tcttcttatt attttaggac agcgaatgag tatggcaatt
     3661 atgcttcagt atttcaaaca tgtgttaact ttaccaatga atttttttgc tacgagaaaa
     3721 tcaggggaaa ttatttctcg ttttcttgat gccagtaaga ttattgatgc tttagcaagt
     3781 gctacgttat caatattttt agatattggt atggtgttgc ttgttggggt tacgttagct
     3841 attcagaata gtacgttgtt tttaatcact ctatgctctt tgccttttta tgtagttgca
     3901 atattcgctt tcgtgaaaag ctatgaaaaa tcgaacaggg aagaaatggc agccggtgca
     3961 gctgtaaatt ctagtatcat agaaagtctt aaaggaatag aaacaattaa agcatatagc
     4021 ggtgaagaga aagtatataa ccgtatagac catgaatttg ttaaattaat gaaaaaatca
     4081 tttcgtacgg ttacgcttga taatattcaa caaggaatta aacatttgat tcaattaatt
     4141 agcagtgcat taattttatg gctaggttct tattttgtta tggataatag aattagtcta
     4201 ggtcaactta tcacatacaa tgctttacta gtatttttga cagatccttt acaaaacatt
     4261 attaatttac aagtgaaaat gcaaacggct caagttgcaa ataatcgttt aaatgaaatt
     4321 ttcgcgattg attcagaaaa atcgaaccag tcttttgaat atatgctttc tgaacaatta
     4381 tttcaacaag atatcactgt aaaaaacgtt tcattctcgt atagcatgaa ggccccaacg
     4441 ttgaacgaaa tttcatgtac aattccttcg cacagtaaaa cggctatagt tggagttagt
     4501 ggttctggaa aatcaactct tgcaaagtta tttgttcgtt tttatgaagc atcagaagga
     4561 tcgatccatt atggaaacat taatcactta gacattcctt ttcagatatt acgcgatcga
     4621 gtgacatatt tgcctcaaga gtcatttttc tttagtggaa caatcattga gaatcttata
     4681 ttcggtttag atagcccacc aagtttcgaa caaataatag aagtttgtga gatagtccaa
     4741 ctaaatgaat ttattaatca acagccatta cgttacgaga cattgttgga agaaggtgga
     4801 aataatcttt caggagggca acgacaacga ttagccttag cacgggcttt attaaaaaat
     4861 ccggacatat taattttaga tgaagcaact agtggattag ataccttgtt agaacatgca
     4921 attatgaatt atctcctcaa tttaaaagaa aaaacgatta tttttattgc gcatcattta
     4981 ccaatagcaa aagcatgtga tcaaatttta gttctccatg aagggcattt ggtagaaaaa
     5041 ggaacgcacg atgaacttcg ttttgccgat ggtatctatc aaaaattatg ggaaatctag
     5101 gagagggaaa caatttatgc agaataataa ttggttcgaa cattctagta tctatactaa
     5161 acaacatcat agcttctacc gttgggtgtt ataccctgta attctttttt tatgtattat
     5221 tggattgttt ttaacctttg ctaaaaaaga ggttgttatt cggacaacgg ctcaattgaa
     5281 ccccgaaaaa atagagaagt tacaagtgcc attagaagct aaaataatgg aaaacttcct
     5341 aaaagaaaat caatttgttc ataaggacga tgtgttggtt agattggatt gttctttaat
     5401 agagaatgaa aaggctcaga tagaacaaga aaaccaacgt attactcaac aaataaagat
     5461 ggctcagcta tttattgaaa gtataagtaa aggaaaaaat ttgttttcaa cggatgacag
     5521 ttttggctac agtaatcaat taaagagcat gttgtcagaa aaagaatcac tccgctacgc
     5581 tttgaagcaa agtgaattaa atgatcaaaa gcaattagaa gtatacgaaa agacaaaaag
     5641 acaactagaa aaacaaattg agagttcaga tagtaaatta caagaatggc aacaagtaca
     5701 ggtagcttgg agtaataatc aatcattaaa agatttttca aaagaaatga tggcaaacta
     5761 tgagaattgg caagaacaac taaataatgt ttctgatgat caaaaaaatc aagtgaaact
     5821 gacaatttca gcaagcataa atgaacaaat tgagcaacta aaaaaagaag tagaacagta
     5881 tcagtcagaa aaagctaaat tagttaaacc aactacttct gagaatgaca gaattagtca
     5941 aacggaaaaa ggaaagcaag agctagaaca agcaataaca actgtaaaac aaacagttac
     6001 agagctaaat gaaaaacaag agaagaacca gtcagttatc aaaagtttgg acgatcaact
     6061 ttcaaaagga atactaaaag ctcctgtatc tggaacggtt catttaaatg aagaaacaaa
     6121 gggacaagca gaactagcca aaggaacagt gcttgcagaa atttatccga tccacgaaaa
     6181 atcaaaaatg aaatttacag cacttttacc tgcgaatgaa agtacctata ttaaagaagg
     6241 gatgaaggtt cattttaaat tggatcaaaa aggtggggct ccgattacga tcgacggaac
     6301 tttagatgaa atttcagaaa attctactgc cactgagaaa ggtgtttttt atgcagtgaa
     6361 aggaacgtta aaaacaactc aatcgtttcc gtttcgttat ggattaactg gagagttgtc
     6421 tttaattgta ggagaaaaaa catattggca acaaattgtc gatgtgtttc taaataaaaa
     6481 ataattattt ttactaagaa tataggagga gtttatatga ataaaagaag atggattgct
     6541 gtgggagtag cgtgcggatt attgttacta tccattatcg tcgcgctaat tccaggaaaa
     6601 gataaagagg aagcatcaaa tacaacccta acaggaataa ataagatttt ttacggatct
     6661 aacgaaatca cagaagaaac gttagaagaa ggcgcatcaa ataaaaagat tgtcaaacta
     6721 tccgtcaatg gagttattgc agatacaggt gaaagcaatc tttttagtag ggaacaatat
     6781 aaccatcaga attttttaac acagctaaag aaaattcaag aagacaaagc ggttaaggga
     6841 gtgttattag aagtaaattc acctggggga ggaatatatg aaagtgctga gattgctaaa
     6901 gaaatggcaa aaattaaaaa gctagatatt cctatctata ctgcatttaa aaatacagcg
     6961 gcaagtggag ggtactacat ttctgctggt tcagataaaa tatttgctac ggaagaaaca
     7021 acaactggat caattggtgt cattatttct ggtttaaatt attctggctt attagaaaaa
     7081 cttggcgtaa ctgatgcgac gtataaaagt ggtgcattaa aagatatgat gtcacctcaa
     7141 cacaaacctt cagaggaaga gaataaagtt atacaggaat ttgtgatgag tgcttatgat
     7201 cgttttgtca atgttgtagc aaaaggaaga aatatggata ctaatgcagt gaaagaactt
     7261 gcggatggaa gaatttatga tggtaaccaa gcagtagaaa atgggttggt ggatcaaatt
     7321 ggttactctg aagatgcatt agattcccta aaaaaagaga agaaattaac tgatgcgacg
     7381 gttatagaat ataaaaatga cacaactggc tttgcttctt cttggttagg taataagatt
     7441 gctgaatggc aagggctaaa ggcaactaca tcagatcgtg taatttcagt ttttgaaaaa
     7501 cttggatttt ctgatactcc aaaaccaatg tattactatg gagggctata aaatgaataa
     7561 gaagagtggc attgaaactt tgcctaatta tgtatttgcg ggattttggt tacgattttt
     7621 tgcttttctt attgatttaa tttgtattag catcatcacc catgctacta taggtctctt
     7681 ttacaaaatc atgggactgg aatctgacaa atccattatt actttttatg gtatagcgtc
     7741 attagctatt tatttaacct attttgtact tttgactaag ctgaattcag gacaaacaat
     7801 tgggaaaatg atttttggga taagagtagt gagctttgag gaaaaagagc taagttggca
     7861 aactatcttt attcgtgagt gtatatgtag gtttatatta aaaaattttg tatttcaaat
     7921 tgggtatttg gtggctgctt tcacgcccaa gaaacaacat attggggatt atttttctaa
     7981 cacgtcagtg gttgttattc gttcattaga agctattaac tatggtcaaa aaatttctaa
     8041 ttctaaaaca cttaatctgg agagaattaa atgaataaga gaaaaacaga gtttattttg
     8101 attttaatta gtggaataac agggatttta tgtagtcttt tagcattgat gggtggtatg
     8161 tttctatctt ctattgattc aactacttta aatactacag aactaaccag taaggatata
     8221 gatatgttag tcaatctaag cgggaatgtt gttttatttg gaggaattac cctagttttt
     8281 tcgataatga ttttcataat aggtttttta gtaagaaaaa atcaaaaaac agtggtctat
     8341 ggtattagtt tactcatttt atctattatt ccattttttc ttttaacatt attgtgggta
     8401 attcctggag ttttaggtat tattgcggga ttgatgcttc taataagaaa atcttaatta
     8461 ggcaatgtgt agacaacgtg aaacgattaa tcaattaaaa gctaaatacg ttttgagaaa
     8521 tgataacttg tagtcgatgg aagacatgta gataaaaaaa ctacgtgtct tttatttatt
     8581 ct
//
LOCUS       DACDIG010000096         8488 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.096, whole genome shotgun sequence.
ACCESSION   DACDIG010000096 DACDIG010000000
VERSION     DACDIG010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.096"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            169..318
                     /locus_tag="IV247_002536"
     CDS             169..318
                     /locus_tag="IV247_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAP8778561.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            329..1843
                     /gene="dltA"
                     /locus_tag="IV247_002537"
     CDS             329..1843
                     /gene="dltA"
                     /locus_tag="IV247_002537"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAP8778562.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1840..3045
                     /gene="dltB"
                     /locus_tag="IV247_002538"
     CDS             1840..3045
                     /gene="dltB"
                     /locus_tag="IV247_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAP8778563.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3090..3323
                     /gene="dltC"
                     /locus_tag="IV247_002539"
     CDS             3090..3323
                     /gene="dltC"
                     /locus_tag="IV247_002539"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAP8778564.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            3326..4594
                     /gene="dltD"
                     /locus_tag="IV247_002540"
     CDS             3326..4594
                     /gene="dltD"
                     /locus_tag="IV247_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAP8778565.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4735..5817
                     /locus_tag="IV247_002541"
     CDS             4735..5817
                     /locus_tag="IV247_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAP8778566.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5817..6620
                     /locus_tag="IV247_002542"
     CDS             5817..6620
                     /locus_tag="IV247_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP8778567.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6690..7430
                     /locus_tag="IV247_002543"
     CDS             6690..7430
                     /locus_tag="IV247_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP8778568.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7441..8445
                     /locus_tag="IV247_002544"
     CDS             7441..8445
                     /locus_tag="IV247_002544"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAP8778569.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
BASE COUNT         2734 a         1397 c         1748 g         2609 t
ORIGIN      
        1 aaaagtacga gaaattataa agaaaaaagc agtgatttga tgtgctattt ttataaaagc
       61 tatgtgacaa aactgttagt aaaaaaacgg tttctgtaag ctagacacat gtttcttttt
      121 gtcaaaatta gttacacttt gtgtgaggtt atatgaggag ggaatccaat gaacaaatgg
      181 cgttctaatc caaaattgaa ttattgggga aaattcatca ttcgaacaat tttttatagt
      241 gcagtactgc tgttcctgat ttatctgtat cactataaaa atattcaagg aggaactttt
      301 atctataacg aattttagga ggaagagtat ggaaatcaaa acgattattg aagcaatcga
      361 tgattgggga ataaatgaac ctgatcgagt tgcttatcag gcggatgaga cgcatacttt
      421 tggtgaattg aaacaagcat cagacgcact tgcttattat ttagaaaaaa aggtagaagg
      481 cgatggacca attgtcgttt ttggcaattt ggagtttgaa atgattgtct catttctagg
      541 ggttgtcaaa tctggccatg cctatttgcc gattgaagag catacaccca aggagcgtat
      601 cctatcgatc tttcgggtgg ctaaaccatc tatggtcatc agtatcgggg actggataga
      661 agatattcct tcggttcctg tcataacaaa aaaagaattt caagaaatca agaagatacc
      721 agtaggattt tatcccagaa atagcgtaaa aggtacagaa aatttttata ttatttttac
      781 ttctggtact actggagaac caaaaggcgt acaaatcagt catgataatc tagtcagctt
      841 tgttcaatgg acgatggaag actttgggat caagcctggt atgcatttct tagcccaagc
      901 gccattttct tttgatttat cggtattttc tatttatcct gcattagttt ctggcggaat
      961 gctgaagccg ttaaataaag cagttgttca agactttcgt caattattcg cgacattgcc
     1021 agccttgaag ttgaatgtgt gggtttctac tccatcattt atggatattt gcttgatgga
     1081 acctacattc aatggagaaa atgtgccgga attggacatg tttttatttt gcggagaaga
     1141 attgacgaaa aaaactgcag aaagtctact tgatcgattc ccaaatgcac ggatatataa
     1201 tacttacggt ccaacagaag caactgtagc tatttcaagt attcagattg accagcatgt
     1261 actagatagt tatgaccgac taccgattgg ctacgtaaaa aaagacacga aagtttctat
     1321 tgtccaagaa ggtaaacctg tttcaaaagg agaaacgggt gaaatcatta ttgcaggacc
     1381 aagcgtatca aaaggttacc tgaacaatcc cgaaaagaca gctgctgcgt tcttttgtca
     1441 aaatgactta gcaagttacc atacagggga tgcaggtaga ctagatgaat atgggctttt
     1501 attttatgaa ggacgtatgg atcagcaggt caaattacat ggataccgga ttgaactcgg
     1561 ggatatcgaa cattatctgc tccaagacaa ccgaatcaaa caagctgtcg tcgtaccaaa
     1621 ataccaagga acaaaagtac agcagctggt tgcttttgtc gtattagaaa acaaaacgaa
     1681 acagccggat tttcagttga ctaaatctat caaagaacaa ttactagaag ttgtaatgga
     1741 ttatatgatt cctcagaagt tccattatgt cgatctgctg cctcagacag ttaatggaaa
     1801 aatcgatcga aaaaaattaa cagctgaggt gaatcctgaa tgatgttacc tcatatgatc
     1861 ccttatgcag agccgtttta tttttttctt ttagccattg cgcttgtacc gatcgttcta
     1921 tctttattga tatgggaaaa aagattacca gtttatcaat cagcagtgac acttttcttt
     1981 ttatttgtca gctttggagg taattattgg caacagggag cggcgctgat tgggtatgtt
     2041 ttgtggcaga cgatacttgt atggggctat tttgtttatc ggcaaaggaa aaatcagaca
     2101 ggatggtttt ttgccgccgt tattttagcg ataattccac tgtttttaac gaaagtgact
     2161 cctttttttg aacatggaaa agcatcgtta cttggttttt taggaatctc ttatttaacc
     2221 tttaaatctg tacaaatgat catggaaata agagatggca tgatcaaaac atataagcca
     2281 tttcgttata tccagtttct gctgttcttt ccaacaatct cttctggacc aatcgatcgc
     2341 tataggagat ttgaaaaaga tcagctccat ccgcctgttc gtgataaata tgttgatctt
     2401 ttgggaaaag gcgtccaaaa tatttttgtc ggttttttat ataaattcat tatcggttat
     2461 tattttggtc aagtactgct tcctgtagtg ggacgcattg ccttacagca tggtggtatt
     2521 tcatgggcat tagtaggtta tatgtacgtg tacagtatgt atttgttttt tgattttgct
     2581 ggctatagct tatttgcagt cggtacaagc tacatgatgg gatatcagac accaatcaat
     2641 ttcaataaac cttttttatc atggaatatc aaagaattct ggaatcgttg gcatatgacg
     2701 ctgtcctttt ggttccgtga ttatgtatat atgcgattga tgttcttcat gatgaagaaa
     2761 aaatttttta aaagcaagat cgtaatgtca aatatcggtt attttgcttt gtttcttcta
     2821 atgggtatat ggcatggatt gacgtggtac tacatcgtat atggactgta tcatgcttgt
     2881 ctgatctgtc tgacagatgc atggatccga tttaagaaga agcataaaga gcagttgcct
     2941 tctaacaaat ttacgcatgg cttagccgta tttttgacat ttcaagcagt atgttttagc
     3001 ttcctgattt tttcaggatt tttagataaa ctttttttta gctaacgaat agtgacatat
     3061 taatttatat aacaaaggag aaaaggaaaa tggaagaaca aattttatca atcttagaag
     3121 aaataacagg aacagatgaa gtaagaaaaa atcgtgatat tgatctattt gatgaagggt
     3181 tgcttgattc tttagcatct gttcaattat tagtcgagct agacggagag ctggatatcc
     3241 aagtccctgt atctgaattc gagcgagagg attggagcac accgaataaa atcattcaac
     3301 aagcaactgc attgaagggt tgagtatgaa aaagaaactt tttgctattt tcggcccggt
     3361 tgttttagct gttggcttac tgtttgtgtt ttttacctcc tcctatcaag tagatatctc
     3421 taatccagaa acgattagac gtgcctcttc ttccatgtca gtgaacgtgt tgaaaggaga
     3481 tgcgataaaa aattatgcat tatctgaaaa acaatatatt cctttttttg gctcaagtga
     3541 attaagcaga atcagtccat ttcatccgtc tgttctggca gaaaaatacc agcgaaacta
     3601 tcgtccattt ttgctgggag cacccgggac acaatcgtta agccaatata tgatgatgcg
     3661 ttctgcagga gatgctatga aaaacaagaa agtcgtcttt attatttctc ctcagtggtt
     3721 cgttaaaaat ggtgtaaaaa ctgattactt taatacgtat tattctgaac ttcaaacata
     3781 cgactggttg ttttcaatga agaaagtaac gcctgctgat cgctatttag ctagaagatt
     3841 attaactttc tcaaaagtaa aagagaatga tacgttgacg gcgattctgc aaacgatcaa
     3901 aaaagggaag ctacctttac ctgaatcttt gaatcagctg cgcagccaat ggaacatgct
     3961 gaagagagag gatgaagtat tcagcaatat tgggttgaaa gatcgacagc aaaaaatcga
     4021 tcatgagtca aaacgattac caaaacaata ccaagaaaca gaattatcca ttttagcaaa
     4081 tcaaattgga gaacgagaaa cgacgaataa tccattcggg ttgaaaaacg atttttatac
     4141 gcatcgtatc cgagcccacg agccagagct caaacagtca caaaaaaatt gggattatcg
     4201 tttttcgcca gaattttctg attttcagct ggtcttggat caattagcaa aaaaccataa
     4261 cgaagtattg ttcatcatcc caccggttaa cgagaaatgg agcgattata ccggactatc
     4321 tcaagaaatg ttacaaggat ttgccaaaaa gatcaaattt caattaaata gccaaggatt
     4381 caatcgaata gctgattttg tcaatcaagc gggtaccaac tattttatgg aagacacgat
     4441 ccatctaggc tggaaaggct ggttggctgc agatcaacag atcagaccgt ttttagaaga
     4501 aaatcatatt actgctagta agtatcatct tgacgatgct ttttttagca aatcttggca
     4561 acatcaaata ccagataaac tacaattaaa ataaagaaaa caggcgagtg ttgctcgctt
     4621 gttttcttta tttttgaata ttctgaataa aagataaaaa taccaagttt gcctgaacaa
     4681 aaatcataag caatggtata atcacaatat gaatatagaa atgggggaaa acatttggat
     4741 acaaaagaag aacagctact tattgattta acttctgcaa aaggagtacc tggaaatgaa
     4801 gaagaggtaa gagaagtatt cagagaatat gccaaaccat tcgctgatga tattttttat
     4861 gacggtttag gcagtgtgat tgccaaacat ggtgctggcg gaggccccaa ggtctttatt
     4921 tctggacata tggacgaagt tggttttatg gtaacaaaaa taaccgaaaa aggattcttg
     4981 gaattccaaa ctcttggagg ttggtggggg caagtcatgc ttgctcagca agttgaaatc
     5041 aaaacacgtg aagggaaaat cgtccacggt gtgattggtt ccaaaccacc acatgtttta
     5101 acacctcaag taagaaataa accttatgaa ataaaggata tgttcattga tattggcgca
     5161 tcaagccaag aagaagccaa agagtggggc atacgtcctg gagatatggt cactccatat
     5221 attgaataca aacgaatgaa tggcagtaaa tatttactgg caaaagcatg ggataatcgt
     5281 atcgggacag ctgtttctct gcgagtatta gagaatctat ctaaagaagc acacccaaat
     5341 gttttattcg caggaagcga tgtgcaagaa gaagtaggct tgcgaggtgc tcgaacaagt
     5401 acacaccttg taaatcccga tattgctttt gctttagata ctggtacagc aggagacact
     5461 ccaggaatga caccaaaaga agcagattcg attttaggaa aaggcccaca gattttgatt
     5521 tttgatgcat ctatgatccc gcataaaaaa ttattgaatt ttgttattga tgtagcagag
     5581 gaaatggata ttccattcca atatacagtg atttctggcg gaggaactga tgcaggacaa
     5641 atgcatttga caagagatgg tgttccgtct gtagccatta ccgttcctgt tcgttatctg
     5701 cattcccata catcagtgat tcatgaagat gattatttga atacagtgaa actagtcaca
     5761 gaattagtga aacggcttga tagtgaaaca gttgaaaaaa ttcggagtta tgaataatga
     5821 ataatagaat acctatttct gaatatcgga aaaaagcacg agtttctttg gaaaatcaat
     5881 ggggaatcaa tacttggctt gttttcttat ctataatatt tacgagcgtc atccagaact
     5941 tttttgacgg tttcagtcct tttccggaag aaagcatgca gcagcactta ttgagttttc
     6001 tagtcgataa tttactttta ttcgcattca actatgcatt gttttacatt gcgctagtag
     6061 ttgtacgggg tggcagacca aaaagtaacc ttttattttc agttttccaa agaaagtatt
     6121 ttggttcgat acttggcatc aatttgatca attcagtcat caactggcta ctcaacagtt
     6181 taatctttct gcccatgttt ttaatcagcg gagtgagtac ttatatgcag ctgcttttca
     6241 gttcaaatgc ggtacctacg ggtgtcatgc cagaagattt tcttgatatc agctttgttt
     6301 tatcgatgat agggatgatt tttgtttcag cactcatcat gagtattgtt ggcggtttat
     6361 tccaatttgc tgcatggaca aagttcgact atcctgattt gtcggttatt caatgtttga
     6421 aatatggctg gtttttattg aaagatagaa ttggtacgta tattttgttg cagctgtctt
     6481 ttatcggctg gtatttgcta ggagcaatcg ctttgttcat cggtcttttg tgggtcattg
     6541 tttatgtgaa tgtgtcagtt gcagaatttt atgaacaagc acggattgaa aaaggaggtc
     6601 cagacgccta ttttacttag catgtaagca gtttattcat caaaaaaata acttttgtgg
     6661 taggataaat ctttgagggg gaatgtacga tgaaattaac tgttttagga tgcttaggag
     6721 cgtatcctta taaaaatcaa gggacaacag gttatttgct tcaatctgac gggttcaatt
     6781 tgttgatcga tgcaggtagt gctacgctga tcaaattaca agaacacctt gatccacttg
     6841 atctagatgc tgtgatcctt agccattatc atcatgatca tattgctgac cttggtgtat
     6901 tacagtatta ctggcaactt catcctaaga gaaatccgga gacattgctt tcaatttatg
     6961 gacacacaaa agattccatt cactttgaag aattgacgtt ggaaaatgtc tcaaaaggac
     7021 atccttattt tgaagctgaa gaattaaaaa ttggtccttt tctgattact tttatggaaa
     7081 caatccatcc agtagtatgt tacgccatgc gtatcgtaga agaaacaacg gggaaagtat
     7141 tcgtatttac aggtgattca ggttatttgg aaagtttcgc tgattttgcg aaagatgctg
     7201 atttgtttct agcagatacc tatctttttg aaggaaatga acatcatcat gcccatttca
     7261 cttcaaaaga agctggagaa cttgcgaaat cagctaacgt aaagaaactt gtacttacac
     7321 acctacctca gtttggtgat ttagaacaat tgaaaaatga agcgcaaaag gcagcaggca
     7381 aagggattga tatagaactt gctgcagttg acaaagtgtt taacatttag gaggaagaac
     7441 atgatttttg taccaaatga aaaccacgac ccaagaatca atttagcaat tgaacaatat
     7501 ttgctgcaag aaatgccttt agatgaacct attttgctct tttatatcaa tgaaccttca
     7561 atcatcatcg gcagaaacca aaatacgctt gaagaaatca atcgagatta tgtggaagaa
     7621 aatggcattc atgttgtccg tcgcttaagt ggaggcggag ctgtctacca tgattttgga
     7681 aacctgaact ttagttttat catgcctgat gatggggatt cattccgtga ctttgcgaaa
     7741 gtaacacagc caatcatcca agcacttcat gaattgggtg ttgctggggc ggaattgaaa
     7801 ggccgaaatg atctagtcat tgatggcatg aaattttctg gcaacgcgat gtatgctacc
     7861 aacggtcgga tgtttgctca tggaacaatt atgttcgact ctgatatcaa tgaagtagtc
     7921 aatgcattaa aagtaaaaaa agacaaaatc gaatcaaaag gaatcaaatc gatccgttct
     7981 cgtgtaacga atatcaaacc atttcttcca gaagaaaagc aaaaaatgac aacagaagaa
     8041 tttcgtcaag aaatcttgtt gaaaattttc ggcgtagagt cagtagaaga agtaaaaacg
     8101 tacgaattga ctgatgaaga ttggaaaaaa atcaatcgaa tctctgagga atattatcga
     8161 aattgggatt ggaattacgg gaagtctcca gatttcaatt tctctcgcca aaaacgtttt
     8221 tctatcggtt cgattgaagt ccatttgaat gtctcagaag gaatgatcaa agaagcaaaa
     8281 atctttggcg atttcttcgg actaggagat atctctgacg tggaaaaaaa attggttgga
     8341 cagaaatatg ataaagcatc tctatcaaaa atagtcagcc aaattgatgt gaagaaatat
     8401 tttggtgcga tagaaccaga agaattgctt accttacttt attgaaaata aaaaatagcg
     8461 gaatccacat atatggattc cgctattt
//
LOCUS       DACDIG010000097         8388 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.097, whole genome shotgun sequence.
ACCESSION   DACDIG010000097 DACDIG010000000
VERSION     DACDIG010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.097"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..591)
                     /locus_tag="IV247_002545"
     CDS             complement(<1..591)
                     /locus_tag="IV247_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP8778570.1"
                     /translation="MRKKTFYQLFGKRILDILLSGIALIVLSPIILIVGFLVRIKLGS
                     PIIFKQERPGKSEKIFSMYKFRTMTDERDHNGEYLPDEIRLTKFGKILRATSLDELPE
                     LWNILKGDMSIVGPRPLLVEYLPLYSEKQRKRHNVRPGLTGLAQVNGRNAISWEEKFD
                     LDVYYVDKISFFNDLIIIIQTCKKVIKKENINTINNN"
     gene            complement(584..1702)
                     /locus_tag="IV247_002546"
     CDS             complement(584..1702)
                     /locus_tag="IV247_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAP8778571.1"
                     /translation="MENKRILLASPHMSEEGYEQEYVKEAFDTNWVAPLGANVNGFEK
                     ELAEYVGASHAAALSSGTAAIHLALKAAGVGKDDIVICQSLTFSATANPILYQGAIPV
                     FVDSDYETWNMKPEHLRTALEKYPNTKAVLVVHLYGLSANLDEITKICKEYDVTLIED
                     AAESLGTTYKGKYTGTFGDYGIYSFNGNKIITTSGGGMLVSNEKEKVEKVRFWATQSR
                     EKARHYQHNEIGYNYRMSNIVAGIGRGQLKVLNERIEKKRYIHNFYKKELSKLSNIQF
                     MPENNWNEPNYWLSVITIKGINTNHLIDELDKNNIEARPVWKPMHLQPVFKNYDYIGD
                     DVAETLFDLGVCLPSDTKMTDEDLKYVVSTIKRVINDA"
     gene            complement(1704..2336)
                     /locus_tag="IV247_002547"
     CDS             complement(1704..2336)
                     /locus_tag="IV247_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP8778572.1"
                     /translation="MKAIIVGAGGFGKEIAFLLQSVSRYELVGFVDDSLKMQNQELLE
                     KPILGTIDLLIELEEETAIFLGIASPDIKEKIYQKINKNNKLIFPNLVAPSALVGINV
                     QLGIGNILMPYTTYTADVVLGNFNMINIGSTIGHDTQIGNYNSVFPSVNISGHVILGD
                     KNEIGVGTKIIQNLSIGNTNIIGAGSVVIKNINNNTKNVGVPTKVIERWD"
     gene            complement(2347..4164)
                     /locus_tag="IV247_002548"
     CDS             complement(2347..4164)
                     /locus_tag="IV247_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8778573.1"
                     /translation="MFVLTRRRKIATLVFVDSLLLIAANIAAAKFMKPFVAIPMDLIL
                     ISIGLSIGFYLFYGSLFKVFTRINRYTNLREIIAVFGSLSASAVSSIIILLFINRRYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYVETKNMRYLSTDSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIHVVGFVDDDPNKRNTYLSGKKVLGALKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESARVKVNTMPSIEELASGKISVSKLKTIDVVDLLGRDEVKLDIESI
                     KDQITDKVILVTGAGGSIGSEICRQIIQFNPAKLLLLGHGENSIYLIDRELRTHHQNC
                     PTEIVPIIADIQDREKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAARVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAQGGPITVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSIQKVMDFVKSLPQDDEQLAKDIVTFANASNK"
     gene            complement(4177..4941)
                     /locus_tag="IV247_002549"
     CDS             complement(4177..4941)
                     /locus_tag="IV247_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAP8778574.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPIYEEVKKKKFGLF"
     gene            complement(4969..5667)
                     /locus_tag="IV247_002550"
     CDS             complement(4969..5667)
                     /locus_tag="IV247_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAP8778575.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQVLAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(5679..6458)
                     /locus_tag="IV247_002551"
     CDS             complement(5679..6458)
                     /locus_tag="IV247_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAP8778576.1"
                     /translation="MEETISLKEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(6474..7418)
                     /locus_tag="IV247_002552"
     CDS             complement(6474..7418)
                     /locus_tag="IV247_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8778577.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(7464..8243)
                     /locus_tag="IV247_002553"
     CDS             complement(7464..8243)
                     /locus_tag="IV247_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778578.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKIRAAYATKAQTVAQQMGYSNISY"
BASE COUNT         2269 a         1737 c         1417 g         2965 t
ORIGIN      
        1 attgttattg attgtattaa tattctcttt cttgattact tttttacatg tctgaattat
       61 aataataaga tcattaaaga aactaatttt atcaacataa taaacatcta aatcaaactt
      121 ttcttcccaa ctaatcgcat ttcttccatt gacctgagct agtccagtta atcctggtct
      181 tacgttgtgt cttttccgtt gcttctcact atatagtggc aaatactcta ctaataatgg
      241 acgaggtcct acgatcgaca tatcaccctt caaaatattc caaagttctg gtagctcatc
      301 tagacttgtt gctctaagta ttttcccaaa tttagttagt cgaatttcat caggtaaata
      361 ttctccattg tggtctcttt cgtcagtcat tgtacgaaat ttatacatgg aaaaaatttt
      421 ttcacttttt cctggtcgtt cttgtttgaa gataattgga gaaccaagtt taattcttac
      481 taaaaaacct acaattaaga ttatcggcga taaaacgatg agagcaatgc ctgacaatag
      541 gatatctaaa attcttttcc caaaaagctg atagaaagtt tttttacgca tcattgatca
      601 ctcttttaat cgtagatact acatatttta aatcttcatc tgtcatcttt gtatctgatg
      661 gtaaacatac tcctaagtca aataaagttt ctgctacatc atcgccaata taatcataat
      721 ttttgaaaac aggttgtaaa tgcataggtt tccatactgg gcgggcttct atgttatttt
      781 tatctaactc atcaataaga tgattcgtat ttatcccctt tattgttatg acgcttaacc
      841 aatagttcgg ttcattccaa ttattttctg gcatgaattg tatatttgac aatttagaca
      901 attctttttt ataaaaatta tgaatatatc gttttttctc aattctttca tttaaaactt
      961 tcaattgtcc tcgaccgata cctgcaacga tattactcat tcgataatta tagccaattt
     1021 cattatgttg ataatggcgt gccttctcac gagattgtgt agcccaaaat ctcacttttt
     1081 ccaccttttc tttttcatta gacacaagca tcccgccacc tgaagttgtg attatcttat
     1141 ttccattaaa agaataaatt ccatagtcgc cgaatgtacc agtatatttt cctttatatg
     1201 ttgttcctaa tgattctgca gcatcttcaa ttagagtaac atcatattct ttgcatattt
     1261 tagtaatttc gtctaagtta gctgataaac cgtacaaatg tactactaat acagcctttg
     1321 tgttaggata tttttctagc gctgttcgca aatgttcagg tttcatgttc caagtttcgt
     1381 aatcactatc aacaaataca ggtatagctc cctgatagag aattggatta gcagtagctg
     1441 agaacgtcaa ggactgacaa atcactatgt catctttacc gaccccagcc gctttcaaag
     1501 ctaaatgtat agctgctgtt ccagaagata atgcggcagc atgactagct cccacgtatt
     1561 ctgctaattc tttctcaaat ccattcacat tcgcacctaa aggagccacc caatttgtat
     1621 caaatgcttc ttttacatac tcttgttcat atccttcctc actcatatga ggagatgcta
     1681 ataatattcg tttattttcc aacttaatcc catctttcta ttactttggt aggtacgcca
     1741 acatttttag tattgttatt aatatttttt attactacac ttcctgcacc tataatattc
     1801 gtatttccaa tacttagatt ttgtattatt ttagtaccta ctccaatttc atttttatct
     1861 cctaatataa catgtcctga aatatttaca cttggaaaaa ctgaattata attaccgatt
     1921 tgagtatcat gtccgatagt agagccgata ttgatcatat tgaaatttcc taaaactacg
     1981 tcagcagtgt aagtcgtata gggcatcaaa atattgccaa ttcctaactg aacatttatg
     2041 cccactagtg cacttggtgc aactaaatta gggaaaatca atttattatt tttatttatc
     2101 ttctgatata ttttctcttt gatatctggc gaagcaattc ccagaaaaat agcagtttct
     2161 tcttctaatt ctattaacaa gtctattgtt cctaatatag gtttttccaa taattcttga
     2221 ttttgcattt ttaacgaatc atcgacaaaa ccaaccaatt catatctaga gacactttgt
     2281 aatagaaaag caatctcttt gccaaatcca ccagctccta caataattgc tttcacttta
     2341 ctccccctac ttatttgaag catttgcaaa agtgacaatg tctttagcca gttgttcatc
     2401 gtcttgcggc aggctcttga caaaatccat cactttttgt attgagtaac ctttgatgtt
     2461 tcctacaaag attttctcat aaactgcttc atcatttctt tccttatcca acaatagttc
     2521 ttcatatagt ttttcccctg gacggatacc tgtttcaatg atttcgattt cgtcttcgct
     2581 atacccgctc aatcggatca tgtttttggc taggtctaca atcttcactg gttcactcat
     2641 atctagaata aaaatttcgc caccttttgc taatgcgcca gattgaataa ctaaacgact
     2701 cgcttcagga atcgtcatga agtatcgggt catacgaaag tctgttacag tgattggacc
     2761 accttgagca atttgttctc tgaatactgg aatcacacta ccgcgagaac caaggacatt
     2821 cccaaatctg acagctgaga atttcgtaca gccttcttca ttcaatccag tcacgatcat
     2881 ttcagcgatt cgttttgttg aacccatgac gtttggagga ttgttagctt tatctgtcga
     2941 taccatgacg aagtttttca ctcttgctgc tttggctgct tctgctacat tttttgtacc
     3001 gaaaatattg tttttgactg cttcttttgg attatattcc atcagtggta catgtttatg
     3061 agcagctgca tggtagacga tatctggatg atattgttcc atgatctcat tgattttctc
     3121 gcgatcttgg atatctgcga tgatcggtac gatttctgta ggacaatttt gatgatgcgt
     3181 gcgtaactca cggtcaatca agtagatcga gttctcccca tgtcctaaaa gcaatagctt
     3241 tgcaggattg aattgaatga tttgccgaca gatttctgaa ccgatcgatc ctcctgcgcc
     3301 ggtgacaaga atgactttgt ctgtgatctg atctttgatg gattcaatat ccagctttac
     3361 ctcatctctt cccagtaaat ccactacgtc aattgttttt aattttgata cgctaatttt
     3421 tcctgaagca agttcttcta tagaaggcat cgtgttgact ttcacacgag cggattcgac
     3481 taactcgaag attcttcgta atttttttct tgataaggaa gggatagcga tcgttaccat
     3541 ttgaatgtca tatttttcaa tcaattccgg aatatctttt aatgcaccta atactttttt
     3601 tcctgaaaga tacgtatttc ttttatttgg atcatcatct acaaacccga caacgtggat
     3661 atcttgagcc gttttggatc caagaaagct gttgtataaa atacgcccgc cttcgccagc
     3721 accgacaatc aatgtatttt tagcagaatc tgtggataga tatctcatat tttttgtttc
     3781 gacatagata cgccagatca atcggctacc aataatcaat aatagcgaaa gcaggtaagc
     3841 aaaaatgact aaacgtaaac tgtatcgccg atttatgaac agcaatatga tgatacttga
     3901 gacagcactt gcagatagtg agccaaaaac cgcaatgatt tctcttaaat ttgtatagcg
     3961 attgatccga gtgaatactt tgaataaact accatagaat aaataaaaac caatcgacaa
     4021 accgatagag attaaaataa ggtccattgg tatagctaca aacggcttca tgaatttagc
     4081 tgctgcaata ttcgcagcaa tcaacaacaa gctgtccaca aataccaatg tggcaatttt
     4141 tcttcttctt gttaatacaa acaccctaca ttccccctaa aataaaccaa acttcttctt
     4201 ttttacctct tcatagattg gataactgat ttcatccccg ttgatcagat cctttgcgac
     4261 ttgtttcatc tgtactactt tttccttacc aaagtccttg gcaatctgtt catagcactc
     4321 ttttaagtaa aaggtgcgct tagagacccc atgggcatca gaagcagcca tctgcacaag
     4381 attatgtttg accatcgttt tggctgtttt ctggatgtct ttgccaaata ccccaacata
     4441 acttggcgca gtgagttggg ccaaacatcc catatctagg aacggaagca aatgatttgg
     4501 gtcttcgcga aaatgcgcgt tacgttctgg atggacgatc actggtgtct ttcctagttc
     4561 taacaactca aaaaaaagcc gttctgcata taacggcact tccagtgtcg gaaattcgat
     4621 cagaagatat gtatcatcta aatctgtaaa taaaatttca tctcggtgga tgtcttcgat
     4681 gagcgttccg gtaatacgta cttcttgtcc ttccaataac gtcaaaggca actgtctttt
     4741 ctctaattcg gcttgtaacg aagcgactag agaaatgact tgtgattttt cattgctgta
     4801 ttttccatta ttgtggtgtg gtgtgcataa aatgtgcgtg atgccttgct gaatcgcttt
     4861 ttcagccatt gcaatacttg cttctaagtt ttctgccccg tcatcgattc ctggtaggat
     4921 atggcaatgt agatcgatca tttctcttgt ctcctctctt cttattcctt aatttccgta
     4981 gtaataacca gcatctttgc tatgttctgc tccgttgtac acaacaccta agactcgtgc
     5041 ttgtaccatc tccagcagtt cttttgcttt agtcaaggat tctttacgtg acatattttc
     5101 acgtacgacc aataaagtgc catccgtttt tgaagacatg atctgtgcat ctgttacggc
     5161 tactactggt ggcatatcga atatcaccac gtcaaataac tgtcttgctt cggctagaac
     5221 ctgatccatt cttggcgagc ttaacaattc tgatgggttg ggtggcttag gtccactagg
     5281 caaaattgac aagttctcga ctggcgttcg ttggatcaca tctgctacac ttcctgatga
     5341 actaagtgct gtacttaatc cactggcatt gttcagctgg aatgtcttat agacgacggg
     5401 tttacgtaaa tctgcatcga ctagtagaac tcgttgacct gatttagcaa aaacgactgc
     5461 aatatttgct gcagtggtgg atttcccttc tccaggacct gatgaagtca cgacaatcgt
     5521 tttgatttgc tgaccagctg cggaggcaaa ctggatattc gtccggatcg tacgatattg
     5581 ttcggcgatt ggtgaagatg gatttgttaa tgtgatcaaa ctgactgcat gagtttgtgc
     5641 tgctttttgt ttctgtgttc gtgccatttt tcttcctcct agactcttga gcggcttctg
     5701 cgtgatgggg ctgttttatc gtttgtagct tttggtcgtg caggagctgt tcctttttta
     5761 cggataccag cgttcaattc tttcgctgac atttctggaa ctgttcctaa gattgggaat
     5821 cctaatgtat ccgtcacata tttttcatct ttcactgtcc gatcaagtaa ttcgaataag
     5881 aatgccaaac ctacgcctac catcattcct aacactaagc caatcactag attcaattta
     5941 ttatttggcg aaacaggtgt cgtattggca actgcacctg aaataatcga gattttatcg
     6001 acattcatca cgtctttggc gttttcttgg aaaacttgtg aagttgtgtt agcgatctgt
     6061 tgagcagtca ctgcattcgt gcttattgcc tggatcgaga acatttgtga attttgtgac
     6121 tgattgacgg agatcgcatc tttgatttgt ccagcagtca ttttcacatt gtcttctgtt
     6181 tctaaacgat ctttgacttc atttagtact aaatcactga cgatcatatc tttatacgta
     6241 ttgatcattt gcaggttcgt attgacatca ttgacatttg ctgtgtctga ttgtggcagt
     6301 gtcacgatca attgtgcttg tgaactgtat tttggtgtca agatgaaaaa cgtcacgatt
     6361 cctgagatgg ccaagccagc aaacattgag atgatgattg ttaccaagtg tttgcgtaat
     6421 gtttcaaaaa tttctttcaa gctgattgtc tcttccattt tttctccact ttcctattta
     6481 ttctctagat tcatttgatc ttttaattct ttttttacac gatcaagttc ttgatcactg
     6541 actttttgat aggagatatt atcttgcatg aagccttctc cttgcagctg atcttgcttg
     6601 atcgtaccaa atgcctcccg ataatctaaa gcaatttttt tcatttcatc aaagctcatg
     6661 tctgttttca tattcgactc tagagcattc aaaacggatt gatagttgct gacaccgttt
     6721 agactcaata ctttttttgc tacgccttct acgatctttc tttgacgctc ttgtctacca
     6781 tagtcaccgt ttggatcttc ataacgcata cgagaataag ataatgcctg atccccgttc
     6841 aaagtaattt taccgattgt aaagtcatag ccatcttgtg agaaagtgag gtcattattg
     6901 acttcgatac caccaactgc atcaacaagt tcttttagcc ctttcatgtt gatagagaca
     6961 tagtgatcga ttgggatgtc tacatatttt tgtacggtat ccatcgccat ggcagctccg
     7021 ccgaatgcgt aagcgtggtt gatcttgtct tttgtgtcac gacctacgat gtcgacatac
     7081 gtatctcgag gaatgctgac gatcgttgtc tgtttgtctt caggactgac tgtggcaacc
     7141 atcatcgtat ctgatcttcc ttgatcgacc cgacctaaat ctccagtatc aatccccatc
     7201 aatagaacag agaaagattc ttgtttgtcc agatcaactg gtttcgttcg ttctccttgg
     7261 gttcgttcca ctgattcgta agttttctca atagaatgtg agagatccag atataatttt
     7321 gctcctactc ctacaattgc taatactaat acgataagaa ctgctaaaat aatcagaaat
     7381 atttttttcc ctcttgattt tcttcttcgt cttgccatag tttcctccgt ttgctaacct
     7441 tgcttgaatc aacgttttaa ctgtcaatag cttatattgc tatagcccat ttgctgagca
     7501 acagtttgtg cttttgttgc gtatgctgcg cgtatttttt cattccggat ctgctctatt
     7561 tgactgagca ccgtcaaata ttgatcgtaa gtagcggtgg cagtcacttg accatccttc
     7621 aacatcgtat cgaataactg atctagttgc gtgacttgcg cgatctgagt atcagcgaat
     7681 cctagatatt gcataacaac cgttttccaa ggactatctt taaaaaagct catattttca
     7741 cgtacacgag cgacatctgc acttgtcacc ttttctttga tgatgacatc gtctacagct
     7801 tgctgccagt ttgatacatt tttagtaaat aaatcgttga cagctgtctg gatcttcagt
     7861 tttgattctg cactatctaa ctgtgtcacg attgctttct tctcatcttg gattgcaaca
     7921 gcttctttag gcaattctga ttctttgatg tcgtaatcat ctgctgaaag tttgacagag
     7981 gatagcttat ttgacaaact tgtgattgtt tcttctgtta tttcagggtt tagaaaaatt
     8041 ttttcttttg tactatacag cttgtctact tcttttgata aagaagtcaa ggtgtctttt
     8101 tgattggtga tggcagattc tgcataattg atccgttgct gatgttgtcg ctgattattg
     8161 atatggtaac caaacaaaca gatcgcaata gcacctgcac ctaccgccac agcagtcaat
     8221 gacttgacga atggcttttc catagattca ctcctaagta ttgatgatag ataaattaag
     8281 atataattca cttacataaa aatgacagct ttttatttta aaaaacataa ttaaactaat
     8341 atattcacag aaatatttgt aaacaagttt tccctatata atcatacc
//
LOCUS       DACDIG010000098         8321 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.098, whole genome shotgun sequence.
ACCESSION   DACDIG010000098 DACDIG010000000
VERSION     DACDIG010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.098"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            474..725
                     /locus_tag="IV247_002554"
     CDS             474..725
                     /locus_tag="IV247_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778579.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
     gene            759..1037
                     /locus_tag="IV247_002555"
     CDS             759..1037
                     /locus_tag="IV247_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778580.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            1634..1891
                     /locus_tag="IV247_002556"
     CDS             1634..1891
                     /locus_tag="IV247_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778581.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            2004..2615
                     /locus_tag="IV247_002557"
     CDS             2004..2615
                     /locus_tag="IV247_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778582.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            2794..3351
                     /locus_tag="IV247_002558"
     CDS             2794..3351
                     /locus_tag="IV247_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778583.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(4050..4571)
                     /locus_tag="IV247_002559"
     CDS             complement(4050..4571)
                     /locus_tag="IV247_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP8778584.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            4770..>8321
                     /gene="nifJ"
                     /locus_tag="IV247_002560"
     CDS             4770..>8321
                     /gene="nifJ"
                     /locus_tag="IV247_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAP8778585.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEE"
BASE COUNT         2773 a         1348 c         1774 g         2426 t
ORIGIN      
        1 acaaataatt ggtttattct ggcaggcgta atgctttttt ctagtttcta tttatttagt
       61 aatgctctat tagcacagcg agggaaatat aagtttaaaa tattagatta gatattattt
      121 ttagagggga taaatataaa atgaaaagag gttatttata atggaaaaag tattaccaat
      181 taagagctac tgtattccaa ggcttttcat catagaattt caaaagtaaa gacatgtgtt
      241 tcacaaatag tgtctatcct ggctaaataa ttcttccact atctgcttta atttcagtag
      301 acttttcggc aagttatttg ctaatgagat gaatagagat tacaactcgc ctataaccgc
      361 ttaaaacaag cttttttaaa gtatagttag caagtcgtat tcatatgttc aaagaggagg
      421 gcttatgaac tttgaattca ttccgtgtga acagaacagt agggggaagt aaaatgaaag
      481 aaaagcctat aaataaatgg tcattgtata aaaattcaat gtttggagtt gttatctttg
      541 gtgtttgtgc tggattattc cttttcatat tattacaaac ggtgactctt ttctcttcta
      601 atcctactta tggatggaga gacagtatag ccttagggat gtttgcggga ggatatggat
      661 tcttattgtt gttctcaatt ctcacactaa gtccaatcca tggactcatt caactttgga
      721 agtaagaacg agccttaaac ttttcgttct cggaagaaat ggaaaaacac aacataacag
      781 gcagtatgta tgtggatgat caatggttta tcagcactga tgaggtaaaa atagctgcca
      841 ttaggagaga ttacctgcag aaaattcata aaataaaaga tcctaatggg aaaaaggcaa
      901 ggttaattat aactttgata acatctaatg gtaagaagtt aaaacttgca ggatcatatg
      961 ataatgttaa aaagttcgaa cattggtatc tgcaaaaaga cagcattagt cttaataaac
     1021 aatttgaaaa aaattaatca aagtcatcgg caacacgaat aatttgacat cttgatattg
     1081 tgatataagc aaaaacggca tttctgagac cgaacagcat aaaaaattta aatgttggaa
     1141 tttatataag caaaacccaa gaagctagat gcgatagagc atttaatttc ttggggtttt
     1201 gcttgggaat cagcatatca attaaaatga tcatctcggt atttcaaaag tgggtacgat
     1261 tacaaatagt tggagcaaaa tagtgggtaa aattaccatt ccagtgtctg tcttgcctct
     1321 ttttcgtcaa tcacaaatag acgagattca taggaaaaat cgatacaggg tatgatttca
     1381 atgatattat gaccaattac aaaatagatt gataaagcat tttttgcatt tttataatag
     1441 tttattgata ataatgtatg agatacagta taatgattta aaaggtaacg tgttgtgacc
     1501 gaattaaatg aattaatgcc tattttttat attccttagt ggacaaaaat ggagacagtg
     1561 aagttaggag cattgtatcc tttgttgcgt agttcaaaaa agtaagcgat ttctataatg
     1621 gtcagggtaa acggtgaggg ctgtccacgt aaatcctact attgaaatca caatgatcgg
     1681 acattcaaaa ggtggtgctg aagcagcaaa ttcagtagct ataaatacga atgcgattat
     1741 atttaatcca gcgacagtaa atttatctgc ttatgaatta gactcaaaca catatacagc
     1801 aaacatggta gcttatgttg tcgagggaga tgcattaaat gttgtttttg gaggagtata
     1861 cacctattgg agaattaatc atgttagata gacaatatga tgcaccttag tggagaatga
     1921 tatctcgcat aggtaactca gtaaaaaatc aaggtatgga agcaattatc tctgcattgg
     1981 aagatcgata tagataggcg gatatgaata gtaatagggg gaataaaatg gaatacaatg
     2041 gatcaatgag aattgtacaa attatatttt ggattacatc gggaattgtg atcataggag
     2101 gagttttttt gatgttgcca agcttaatat ttcctttctt tgcattaatc tcacctaaaa
     2161 tacccgaacc ggaaatcacc tatggagaat ttccatttag gattgaatac gaactagatg
     2221 gacagattaa cataattgaa gatactgtca tagctgagtt taacgggtgt gagttcagcg
     2281 caggttctat gaaaagagaa cgaagatgga gaagtagatt agctagtggt agagaagatc
     2341 taccttttgg ggacgatcta ggaatctatt ttagtcgagg aagtgcgcaa tattatatgg
     2401 gggaaaatgt acaatcaatg tcattaaaac cacatatagc ccttagaaat ctcgaggaag
     2461 gttttaaaag agaggtagac tttatattag gagaaagtgg atacataaga actgtactta
     2521 atttcggaga agcgcaagaa gttttgacac aatacggaat taccttaatc aactgggaaa
     2581 tatctgaacc gattgttaac aattttggtg attagaaaac aattacgata aatagaaaca
     2641 agccaaatca tcagcaagta aaagctgatg atttggcctt tcttatacga gtagtaggaa
     2701 ttcatagcga atattattac gaccagtaga tattttgata ttaaaggagt attacgaact
     2761 aatatgtgag aatgtagagg tgatgtgcga ataatgagaa atttatcaaa tagaaacaaa
     2821 atattaatca ttattgttgt tatagctgtg tttcatctgg gaacgaatgc ggttttaagt
     2881 agaataatat tgggtcctaa gccaccaaga ccagaaatta cccgagggga gtttgatttt
     2941 agattagaat atgaagtaga tggggaaagg attgtgatag aagatacaat tgttgcatta
     3001 tttgatggat tttctgcaga tgcaggtagc atggcatggt atcgcacgtg gcgtctacat
     3061 ttagcaagtg atagacgatc acgaaatatc ttattagatg aattagagga tggtcgaaga
     3121 atatattatg tcccagaaag tgctaactat tctatgggag atgtacagaa aaaaagagag
     3181 cctaatccta attggtatcc atttaatggc gtaacaatag aatatccacg caataaaaca
     3241 cctgaaatag gtgcaaaatt tataagtggt ttagaagatc tgtacgatag gtttggaatc
     3301 cgtcttatta gttgggaaca cgatccacca atagaaaatc gctttgaata atcgaaatct
     3361 actaatccat ttcatcagcc agtgaatgtt gtaatggaac aatttggttt agtgttcaaa
     3421 accattgaaa tactaggctg tgatcagcaa aaatgaatct gaataaaaaa gagagcaact
     3481 attagctgag aggagttaat agttgttagc tttatacgaa tcaaagtggc aaatacagat
     3541 gcgtatgcat ttgtgtttgc catcttgcgt ttggtagacg agataggtat aagctaaaga
     3601 aacaacttgt ttaaaataaa cgacctagct caacaaatga ccatctaatc aactaacagc
     3661 atacataaaa gcaactattt ttgaagtaga attgaaatcg ggataactta aaaagtgatt
     3721 gggatactag tgagaatcac cgtacataaa tattactcgt taagggaaat tgagaaattt
     3781 tgtatgaaaa tttaagaatt agttagtatg cgcaagtcaa aaatatgaat aaattatttg
     3841 gtggagataa tttgcttagc ttgtcataag aaatgagata atctggcaga tgcaagcttt
     3901 tggacaccat ttattcggaa taaggaggct gggacagaag cgtttaactc caagaaataa
     3961 gaaggagttg cttctgcttc cgccgtttat acgtgtttag agcgtgagac aaaagtgttc
     4021 tttacttttg tcccacgctc cttatttttt tattttgttt ggatcaattc ttttaattcc
     4081 tctattgaaa tagattcatc aaagatttct ttacccaaga tgacagttgg aacaaatcga
     4141 atatgtgctt gctcagcttc gttgatgaca gcttggctca gctgttcgtc tttttgttct
     4201 gtcagcttta gtttatctac tgcaaaatcc gctacttctt ggagagaaag atgtttccac
     4261 tcgtcttgag tatcgaatat ttgtttgatc tctttgatcg ctttttggcc atcgctgatt
     4321 gtgagatacc ggtgcatcac gttccctcgc tgtaagcttt ctttttcttt atcgaacaat
     4381 ttgatcactc gctgcaattg cccgctttgg actgcttctt ctaatagctc ataagattct
     4441 tcaaaccatt gtcggcaata cggacaagct aagttgatga attcgaccat ttttttagga
     4501 gcattcgatg aaccataaag gattcctttt tgtgtatttg ttttcgttgc atcaatcact
     4561 gatatatcca tgttaaaaca cctctttcgc ttatagttta tacatgtctg acaaaaaaag
     4621 gcaacgattt agactgtttg tctaaaaatt agatgaccaa ttcaaataga tacatgtaaa
     4681 atatttttat aaataaaaga aaaaaatggc atgttttacg ttataatgga acagataata
     4741 agagagaata tgaacaagga tgatgaatca tgcgaaaaat gaaaacaatg gatggcaata
     4801 cggcagccgc atatatttct tatgcattta ccgagattgc tgcaatatat ccaataacac
     4861 ccagttcgac catggctgaa ttagtagatc aatggtcagc agcaggaaaa gaaaacgtat
     4921 tcggtcaacc agtaaaagta gtagaaatgc aatccgaagc aggagcagct ggtgtggtcc
     4981 atggatcatt gaaaactggt gcactgacaa ctacatatac cgcttcacaa gggctgcttt
     5041 tgatgatacc gaatatgtac aagattgctg gggaactact gccaagtgtt ttccacgttg
     5101 ctagtcgggc agtgacgaca aatgcgttga atattttcgg agatcacgga gacgtgatgg
     5161 ctgcaagaca aacaggattt gctatgttag ctgaaagcag tgtacaagaa gtgatggatt
     5221 tagcacctgt tgcccatctt gcttctattg aaggagaagt cccttttttg aatttctttg
     5281 atggattccg tacgagtcac gagattcaaa aaatcgaagt tttggaatat gaagaattag
     5341 ctcctctgat caatcaggag aaattagctt catttcgtaa acgagggatg aatcccaatc
     5401 atccgactgt tagtggtacg aaccagaatc cagacatcca ttttcaacaa cgggaaacga
     5461 tcaaccccta ttatgataaa ttacctgaaa tcgttcaaaa atatatgaaa gagatcaacc
     5521 gattacgagg aacttcttat gacttggtga cttattacgg agcagacgat gcggaagaag
     5581 tcattgtttc tatgggatca gttgcacaga cgattgaaca aacgattgat tacttgaatg
     5641 caaacggacg gaaaacaggt tttttgaata tccatcttta tcgtcctttt ccagtggaaa
     5701 cattcgtgca aaagttgcct catacagtaa aaacaattgc tgttcttgat cgaagtaaag
     5761 aaccaggggc aggtggagaa cctctcttat tagacgttca aagtgcgtta tatgattctg
     5821 aacttcgccc ggctgttatt ggtggtcgct acggattggg gtctaaagat gtgactcccg
     5881 accaaatctc agcagttttt gatgaattga aaaaagaccc aagcatacgc aaaaaacggt
     5941 ttaccatagg gatcgtggat gatgtgactt atcaatcttt ggaaccaaag gaaagtctgg
     6001 atttgacaga accgcaaaca ttccaggcca agttttgggg atttggatct gacggaacag
     6061 ttggagcaaa taaatcagct atcaagatta ttggagatca caccgataaa tatgcgcaag
     6121 gctattttta ttatgattcg aaaaaatctg gcggtctgac tgtctctcat cttcgttttg
     6181 gagatacgcc gatacgttca gcgtatcttg tcgagcatgc ggaccttgtt gcctgtcaca
     6241 caccagctta cctgcatagc tatgacttgg tcaaaggatt gaagccaggt ggcatttttt
     6301 tattgaatac tctttggagc gacgaacaat tagaaacgca tctgccctta aaattaaaac
     6361 gttacttggc tgaaaataac attagattct atacgatcaa tgccatgaga ttggcccaag
     6421 aagtcggttt gggacgccgg atcaatacgg caatggaaac ggcttttttt aaattagctg
     6481 acatcattcc ttttgatgag gtgcttccgc tgctaaaaga agaagccctg aaaagctatg
     6541 gacataaatc aatggctatc gtagaaaaaa acatacaagc aatcgatcgg acgaccgaac
     6601 tgcttcatca agtcgcgatc ccagaccatt ggagaacgtt ggagatgcct gaaaaaatca
     6661 ataaaaggaa cactggttat atacaagaaa tacttgaacc agtgaatgca caggaaggaa
     6721 atcaactctc tgttggtaca cttgtagcaa atggaatgac aactggcgaa atgcctttag
     6781 gaacggcttc tatggaaaag cggggcgtcg ctttagaagt acctgagtgg attagtgatc
     6841 gctgtacgat gtgtaatgaa tgtgcctttg tttgtcctca tgctgccatc cgtccatttt
     6901 tagcagatga agaggaaatg gaagaagcgc cagaaggatt tatcgtgaga gaaatgcgag
     6961 gcgcagacgg cgtaaaatac cgtatccaag tttcagtgga agactgtaca ggttgtggtc
     7021 tgtgtgtaga ggcttgtccc gcaaaagaaa aagctttagt gatgaagcct tatgaagaac
     7081 agaaagaaca agctgtgaat tgggcttttg caatgacatt aagacaaaaa gcaaatccag
     7141 tcaaaggaaa gaatactgtc ttgtctacac aattcaatca accgcttctt gaattctcgg
     7201 gcgcttgctc aggctgtggc gaaacacctt atgtaaaatt attgacacaa atgttcggtg
     7261 atcgaatgat gatcgcgaat gcgacaggct gttcttctat ttggggcggt gcttctcctg
     7321 cttctcctta tacgaccaat gaatgtggtc aagggcctgc atggtcaaat tctttattgg
     7381 aagataatgc tgaatttggt tatggtatgc ttctagcggc acagactcgt cgcgaggctt
     7441 tggcaagttc aatcaaagaa gtattgccaa aagtatcaga agaactacga gaattgatgg
     7501 aagactggtt agtacacatg tttgagagtg aaggaacgca gcaaagagca gcaaaactgc
     7561 aagctgcgat cgaaagcgaa cgatcaattt tccctgaact ggaggatata tacaagagaa
     7621 aggatctatt cgtaaaaaac agccaatgga tgattggtgg agacggatgg gcttacgaca
     7681 ttggttttgg cgggatcgat catgtacttg ccagcggagc agacgtgaat atccttgttt
     7741 tggataacga agtctattcc aatactggcg gtcaaacctc aaaagcaaca ccagcttcgg
     7801 caatcgctaa attttcggct agtggaaagt atgcttcaaa gaaagatcta ggaatgatgg
     7861 cgatgacata tggaaatgtt tatgtggcgc aaattgcttc aggtgccaat caaatgcaaa
     7921 cgatcaaggc atttgaagaa gcagaaaagt atcctggtcc ttctctgatt attgcctata
     7981 cgccttgtat cacacatggc ttgcttggcg gaatgaaaga atcgcttaaa gaagcaaaag
     8041 aagctgtttc ttctggttat tggtcattat atcgatataa ccctacaatg gtcgaaaaaa
     8101 acaagaaccc aatgactttg gactacaaaa aacctgattt ctctgctatg cctgatttca
     8161 tgaggaaaca agttcgcttt tcctcgctag ataacgcaaa tccaaatctt gcaaaagctt
     8221 tatttgaaaa gactgtccac gacgctaaaa cacgttttta caactatgct gcactgacgg
     8281 gagaattaga aaaaatcaag aaacgacttg aaccagagga a
//
LOCUS       DACDIG010000099         7866 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.099, whole genome shotgun sequence.
ACCESSION   DACDIG010000099 DACDIG010000000
VERSION     DACDIG010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.099"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            154..2763
                     /gene="clpB"
                     /locus_tag="IV247_002561"
     CDS             154..2763
                     /gene="clpB"
                     /locus_tag="IV247_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAP8778586.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(2914..3585)
                     /locus_tag="IV247_002562"
     CDS             complement(2914..3585)
                     /locus_tag="IV247_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778587.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(3578..4072)
                     /locus_tag="IV247_002563"
     CDS             complement(3578..4072)
                     /locus_tag="IV247_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP8778588.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(4092..5312)
                     /locus_tag="IV247_002564"
     CDS             complement(4092..5312)
                     /locus_tag="IV247_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8778589.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            5459..7294
                     /gene="lepA"
                     /locus_tag="IV247_002565"
     CDS             5459..7294
                     /gene="lepA"
                     /locus_tag="IV247_002565"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAP8778590.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            7490..>7866
                     /locus_tag="IV247_002566"
     CDS             7490..>7866
                     /locus_tag="IV247_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8778591.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLL"
BASE COUNT         2641 a         1452 c         1588 g         2185 t
ORIGIN      
        1 tcaattttgt ttttttaaga tttatataag tcgagaaaaa aagtcaaaaa actatgtgta
       61 tattttgacc aaaattgacc aaagcgatat aatagttttt gtaaagatca aagaaataag
      121 tgatcttacc tttaattagg agggtatacc tgtatgaata ttgagaaaat gactacgacg
      181 cttcaagaag cgattgctga agctcaacag attgcggtca ctcgaaaaca tcaagatatc
      241 gacattgccc atgtatggaa aatcttttta caatctaatc attttggtcg aaatttctat
      301 acggatgccg gattggatgt cgaatctttt gaacatgaaa ttgatcgtct actggatgag
      361 tatccagttg tttctggtgg aaacgtccaa tatggacaaa acctcagcca gaatttcttt
      421 cgattattga atgaagcaga ccaaatacgt gaaagcttcg gcgacgagtt tctttcaacg
      481 gaagtcgtta ttttagcgtt gatgaaatta aaaaattatc cgttaactgt ttatttgaat
      541 aaaaacggat tatctgaaaa agaattgcgt aaaaacatcg aagagatgag aggaggagaa
      601 agagtgacct cacaaaacca agaagaacaa tacaaagcat tagaaaaata cggcgttgat
      661 ttagtacagc aagtcaaaag cggaaaaatg gatccaatca tcggtcgtga tgaagaaatc
      721 cgtgatgtca tccgtattct ttcacgaaaa acaaaaaata atcctgttct gataggagaa
      781 cccggtgtag ggaaaacggc aatcgttgaa gggcttgctc aacgtatcgt acgcaaagat
      841 gtgcctgaaa atttaaaaga taaaacgatt ttttcattag atatgggtgc attgatcgct
      901 ggagcaaaat tccgtggtga atttgaagaa agactaaaag cagtcttgaa agaagtcaaa
      961 aaaagtgatg gacgtatcct cttgttcatc gatgaaatcc acaatatcgt aggggcagga
     1021 aaaacagaag gaagtatgga tgccggaaat ctgttgaaac caatgcttgc tcgcggtgaa
     1081 ttacatttga tcggtgccac aactttagat gagtaccgtc aatatatgga aaaagacaaa
     1141 gcgttggaac gtcgctttca aaaggtatta gtcaaagaac caacagtgga agatacgatc
     1201 agtattctgc gtggattaaa agaacgtttt gagatccacc atggtgttaa catccatgac
     1261 aacgcattag tagcagcagc tactttatct gaccgttata ttactgatcg gttcttaccg
     1321 gacaaagcaa tcgatttgat cgatgaagcc agtgcaacga ttcgggtaga aatgaactct
     1381 atgccaacgg aattagatca agttactaga cgtttgatgc agttggaaat cgaagaagct
     1441 gcactgaaaa aagaatcaga tgatgcaagt aaaaaacgct tgaaaaattt gcaagaagaa
     1501 ttagctgaac ttcgtgaaga agcaaatgcg atgaagatgc agtgggaaac agaaaaagaa
     1561 gaagtcaatt ctgtttcagc aaaacgtgct gaaatcgata aagcaaaaca cgaattagaa
     1621 gatgcagaaa ataactatga tttagaacgc gctgctgtat tgcgtcatgg tacgatacct
     1681 caattagaaa aagaattgaa ggaattagaa gctaaagcaa aagacagcga gattaagatg
     1741 gttcaagagt ccgttaccga aaatgagatt gctcaagtcg ttggcagatt aacgggtatc
     1801 cccgtaacga aacttgtaga aggtgaacga gaaaaactaa ttaaattgaa tgaaacactt
     1861 cataaacgag tgattggtca agatgaagca gttgatgcgg tcagtgacgc ggtaattcgt
     1921 tcacgggctg gattgcaaga cccaaatcgt cctctaggtt ctttcctttt cctaggacca
     1981 actggtgtcg gaaaaaccga attagcgaaa gcgttagctg agaacttgtt tgactcagaa
     2041 gatcatatgg tacggatcga tatgagtgaa tatatggaaa aacatgctgt gtctcgtcta
     2101 gtcggagctc ctccaggcta tgtaggttac gaagaaggtg gtcaattaac ggaagctgtc
     2161 agacgaaatc cttatacaat cgtcttgttg gatgaaatcg aaaaagcgca tcccgatgtc
     2221 ttcaatatct tgttacaagt cctagatgac ggacgcttga ctgattcaaa agggcgagta
     2281 gtagatttca aaaatacggt tctgatcatg accagcaata tcggttcaca gttactgctg
     2341 gaaggcgtga ctgctgatgg aacgattcca gaagcagtgg ctgaacaagt caacacgcta
     2401 ttacgtggaa acttcaaacc agaattcttg aaccgaatcg acgacacgat tttatttaca
     2461 ccattgagcc tagacaacgt gaaaggaatc gttgacaaaa tggtggcgca attggcacaa
     2521 cgtctggaac atcaagaaat cctgctgaca atcagtgatg aagccaaaac gtggattgct
     2581 gaaaatgctt atgaaccagc atatggtgca agaccattga aacgatttat cactaaggaa
     2641 gtagaaacac cactagctaa agaaatcgtt gcaggacatg ttatgcctaa atcaaaagta
     2701 acgattactt tacttgatgg acaacttcat ttcaaaacag aagagctaga agaaatcgtc
     2761 taatcttcca ttgagactat aaaagaaaaa aggggtctga cacaactagt gtcagacctc
     2821 cttttttcaa ataaacagtg ttccagaagt agtttcttcg aaaaataagc cgaaatttct
     2881 tgaagctgcc cgcttctgtc acaacctctt ttattaggta tttttcactt tttctaattt
     2941 ataactttca aagtaacgct catctttttt actttgagac catgcaaaca atcccattaa
     3001 ggcagcacct atcgtatttg tcagcaagtc gcccatcgta tccatcaatg ctgctcgtcc
     3061 gacaaacagt gtgccatcag aagcagcaaa acgttgaaga ttcatgccta agaactggtc
     3121 gcataagaat tcccagaact cccaaaacac gccgcaaagt ccagcaaacg caaagccgaa
     3181 taagaggaac aaccaaggac tggtttttga tatctcagca tctttcatca agtaaccaat
     3241 caatccatag ccaagagctg tcaggaccat tggactcacc gcatgtaaga ttttgtccca
     3301 aaaagagatg atactgatca aatgcatccc tgtgcctaaa aagacagaga tgaacaagaa
     3361 aaaccagtaa aacaaaacga ttgctgctgg tattttaagt ttgaaaactt tcaaaatgac
     3421 ttgtggcatg aaaatcaatg caatccctaa taaagattcc acgataaatg tgattccttg
     3481 agaagtcgaa tatttttgtg tgataaattc ataaaaatta tagcagacaa cgaatacacc
     3541 aaaagcaagt aaagctattt gcaccttttt gatttcttta atcactgaaa gtccctccat
     3601 ctaaaaatct ttttacttct gaaagcaact caatttctga agaaaccact tttccgttca
     3661 atttgatcaa acctattgta tataagttca ggtagtgaaa ttgattttca gctgtttctt
     3721 gtagtgcagc gatttttttc gggtgggttc cgccttgctg tcgagtatca gtatataagc
     3781 cgatcactgg gatgcctttc gcataagcca caccgatttc cgatgctacc ccggcatcga
     3841 tcgtcagccc atctagtaaa gcaatcatta aatcactagc caacactttt tctgtgtcag
     3901 caagagcaat cattcgactg tctgcataag cagttttgtc attgattgct tcattttctt
     3961 gaggaagata gatcgaaagc tcagaagaga gctctctgat ttctttaact aactgttggt
     4021 tatataacaa atcactttta gcaaacattg gtgcggcaaa ataaattttc atctaaacat
     4081 catccttttt atcaaaaata atcgctaaag taagcttcca tgtttggaat gatacgctct
     4141 agtgacttca gtgtttcttc atctgtttct aaccgttcga ttcttgccaa ataagaagca
     4201 cggcgataat tcatcatcaa gcaagtcaat gtttggatat ccagtcgaac ggattttcct
     4261 ataggttcat ttaagacacg gacttgtccc tgttcatccc atgttaaagc gaagacacca
     4321 ttattccaag gagcgacagg atcttcaatg atgaaatgaa aagcatcagc tgttccaaca
     4381 aacggaaacc gttgaaggaa ttcttttaca tcaacgatcc gagccataaa atatggttcg
     4441 atctgctctt tgatctgact atcttcaatc aaaaatgcta aaggctcatt tttataaata
     4501 tctccatgta cccagtagat catggaaaaa tgtgcggaaa taaagttcca taagccattt
     4561 cgagcttctt gatcaagata gaacatttct tttacgtgaa atatttcttc agctacccag
     4621 tagaacagaa cacctcttgg ttcatggttg ctatcgtagt agacagcggc tgtgcgctca
     4681 tcttcatttt cgaaacgcca atactcttcc caatgaaaat cactgcggaa taaagcacca
     4741 tggttttgtt gggcaaattt gtcataaacg gaaaatacgt ctggatgatc aactggtaat
     4801 cgctcaacca taccacttac tgggatttgt ttaggtagct gtgtatctct gattttaaag
     4861 gaaagtttat cagacatgat ttcccatcct ttacgccgat agtaggggat actgtaagga
     4921 tacaaataag agatccattg cttattcttg cgcatattat ccaatgcaac gataattaga
     4981 tcttgcatca atccatggtt agcatattct ggataagttc caactccagt cacaccaccc
     5041 attttataac gtgcaccgtg aatattgact tcacaaggat agatagcgat ttgagagatc
     5101 agtttatttt cgtggaacca tccgaacact ttcgatactt ccaaaatcgg ttttttggaa
     5161 cggataaaag ctcgtttatt ctcaaacccg ctttcctcga tgtctgcttc agtcacttgg
     5221 aacacatagg aaagcaattc attgaattga tcgatatgtt cttgagtgac cggttttaat
     5281 tcaagatttt ctctaaaatc tttttgatcc attttttcat ctccatttat tagcttgata
     5341 acagtatagc aaaattctac aaaaaaaact tctatagctg actaattaaa aactctttaa
     5401 attactgctt ttcgttggta taatggaaag tgctgattat atgaaaagag gagacgctat
     5461 ggatataaag aaaatgaaag aacgccaaga gcagattcgt aatttttcga tcatcgctca
     5521 tatcgaccat gggaaatcta cattggccga tcgtatttta gaaatgacgc acactgtgac
     5581 gtctcgcgaa atgcaagatc agctgttaga ctcgatggat ttggaacgtg aacgtggaat
     5641 cacgattaaa ttaaatgctg tagaactcaa ttacacagcg aaaaatggag aaacatatat
     5701 ctttcacttg attgacaccc cagggcacgt cgacttcacc tatgaagtct ctcgaagcct
     5761 tgctgcctgt gagggtgccg ttttagtggt cgatgcagca caaggaatcg aagcacaaac
     5821 attggccaat gtttatttag cgttagacaa tgatttggag atcctgccag tcatcaataa
     5881 aatcgaccta cccgcagcag atcccgaacg tgtccgtcaa gaaatcgaag acgtaatcgg
     5941 aatcgatgct agtgaagctg tattagccag tgccaaatca gggattggga ttgaagatat
     6001 tctcgagcag atcgtggaat atgttcctgc accaagtgga gacttagatg caccgctaaa
     6061 agctttgatc tttgactcta tttatgatag ttaccgcggt gttgttctaa atgtccggat
     6121 cacggacggt atggtcaaac ccggagacaa aatcaaactg atgagtaatg gcaaaacatt
     6181 tgatgttaca gaggtcgggg tcttctcgcc aaaagcagtt gctagagact ttttgatggt
     6241 cggtgatgtc ggttatatca cagcaagtat caaaactgtc caagatacac gagtagggga
     6301 tacagtaacg ttagcagaca atcctgccgc tgaagcttta ccaggttacc gaaaaatgaa
     6361 tccgatggtt tattgtggtc tttatccaat tgatacttct cgttacaacg atttgcggga
     6421 agctttagaa aaactgcaat tgaatgatgc tgctttacaa ttcgaaccag aaacctctca
     6481 agcattaggt tttgggttcc gctgtggatt cctcggactt cttcatatgg atgtcgtcca
     6541 agaacgcttg gaacgggaat tcaatctaga attgatcacg actgcaccat ccgttattta
     6601 ccatgttaat aaaacggatg gtacgactgc tacagtagat aacccggcag attttcctga
     6661 acctgtaacg attcaggatg tagaagaacc attcgtcaaa gcacagatca tggtcccaaa
     6721 tgattttgtc ggtgcagtaa tggaactttc tcaacgaaaa cggggagaat tcattacgat
     6781 ggattattta gatgattacc gagtaaatgt cgtctataat attccgcttt ctgaaattgt
     6841 ctttgacttt ttcgacaaat tgaaatcaag cacgaaaggc tatgcctcat tggattatga
     6901 aatgtctggt tatcaaaaaa gcaagttagt gaagatggac atcttgctga atggagaaaa
     6961 agtcgatgca ttaagcttta tcgttcaccg agactttgcc tatgaacgtg gaaaagcaat
     7021 cgttgaaaaa ctgaaaaaac taatcccacg ccaacaattt gaggtgccaa tccaagcagc
     7081 tattggacaa aaaatcgttg cccgttcaga catcaaagcc ttgcgtaaaa acgtcttggc
     7141 taaatgttat ggtggagatg tttctcgtaa acgtaaactc ttagagaaac aaaaagaagg
     7201 gaagaagcgg atgaaacaaa tcggctccgt cgaagttccg caagaagcct tcatggccgt
     7261 tctgaaaatg gatgagcaag atagtgtgaa ataacttata atcaatcgaa aaacctatta
     7321 aataaacctt tttgagtaga tgcaacactg aggattatct gtataaaaga ttttaaactg
     7381 tcatttggtt atcacggcga tactaaaatt aacaaaattg gaatattttt cttgaagatt
     7441 tttagctgtc agtatatgat gaaagcatct cataggaggt gcttttttca tggttgataa
     7501 ttcaagtgga agggttctta aatctctaag aaaagagaaa aaactatctc aaaaaaagtt
     7561 agctgattta gcaggtattt cacaaagtac tttagttaaa tatgaaaaag ggtcaagaaa
     7621 aatccctaaa gacgtagata atactctaag caagattctg aatatagaaa ctctaataaa
     7681 agatgaggaa gataagattg agattctcat cggtaaacta attgcataca gagatatgaa
     7741 taagttactt aacaaagagt tagctgataa tataggaatt agtgaagtat tactcagtta
     7801 tgtgttaaat agaaagagaa atccgtcaaa agaaatgcaa aaaaaaatcg atatttttct
     7861 attaag
//
LOCUS       DACDIG010000100         7837 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.100, whole genome shotgun sequence.
ACCESSION   DACDIG010000100 DACDIG010000000
VERSION     DACDIG010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7837
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.100"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..813
                     /locus_tag="IV247_002567"
     CDS             <1..813
                     /locus_tag="IV247_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HAP8778592.1"
                     /translation="KKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSD
                     NGQIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSG
                     DNFIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIES
                     KSLNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHAL
                     ALFGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            807..1229
                     /locus_tag="IV247_002568"
     CDS             807..1229
                     /locus_tag="IV247_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778593.1"
                     /translation="MVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2399..3367
                     /locus_tag="IV247_002569"
     CDS             2399..3367
                     /locus_tag="IV247_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP8778594.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            3384..3908
                     /locus_tag="IV247_002570"
     CDS             3384..3908
                     /locus_tag="IV247_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP8778595.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKAEKKWWQFWQ"
     gene            4084..4488
                     /locus_tag="IV247_002571"
     CDS             4084..4488
                     /locus_tag="IV247_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HAP8778596.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
     gene            4505..5653
                     /gene="tnpB"
                     /locus_tag="IV247_002572"
     CDS             4505..5653
                     /gene="tnpB"
                     /locus_tag="IV247_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAP8778597.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
     gene            6175..6456
                     /locus_tag="IV247_002573"
     CDS             6175..6456
                     /locus_tag="IV247_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778598.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            6458..6874
                     /locus_tag="IV247_002574"
     CDS             6458..6874
                     /locus_tag="IV247_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778599.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            7496..>7837
                     /locus_tag="IV247_002575"
     CDS             7496..>7837
                     /locus_tag="IV247_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HAP8778600.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTV"
BASE COUNT         2944 a         1320 c         1304 g         2269 t
ORIGIN      
        1 aaaaaaaata caaatggaga atattcagga agtatttcac aatttttagc aaaagaactt
       61 caaaatctag tagatagtaa tataacagat acaaccttat tttttcaaga taatagcatt
      121 tattattccg ataatggaca aattaaacta ctttggaaat cacctttaag tacagaaaaa
      181 aatggtgttt cttcctttac agaaaaaaat gatcttatat ccgtaaatat aaaaaataaa
      241 attaaaaatt tctctaaaaa acaaaatttt cctgcggtta aacgtgtaaa aagtgtctcc
      301 tccggagata actttattat gattaattgg cgtatagcag aaacccaaac aagttttccg
      361 tggtgtgctg cttacgtaga agctgctatc ttatgtaaca aaacagatgt acgtgctact
      421 tcagctagag ccattatgca ttatacctat cctaatttaa acaacagtca aattgaatca
      481 aaaagtctta atcaagatca aataataaaa tatgcaaatt actgtggtgt ttatcctaaa
      541 aaagtagcac gaatactaaa cctttcagag gtccaaacac aactaagaaa aggaaatgcc
      601 atttatatgg gaactgctgg ttatggaaat tatagtaaca gccgtcatgc acttgcatta
      661 tttgggtggg tcaatcaagg tggttatcaa acttactatg tgtggaatcc ttggtataac
      721 tacccaactg taataagtgc aaatgcagga ccagttacaa ttcctgtacc tggaggtggt
      781 tttacttggg acaataccat tacaaattgg taaataaacc cattgcttta gttatgggtc
      841 tagtgttagt tcttttctta actagttgtt caaaaaaaca agaaatacaa aagactgatc
      901 tgattccttt tctaggaact tggcaaacta aagatcattc agaacgtcta gtaatcacta
      961 atttaaatac ttcaaaaaat atagaacaat ataccatctt agtaaaagag aacaatcata
     1021 aaattcaaat cattgataaa aatttaaata taaatgaaca agttttcatt tacaaagcaa
     1081 caaataattt actaacactg cgctctaata aaaaacaatt agattacact tttagtttgg
     1141 attctccaaa aaagctcgta tttattttga caacgaatga tgggacagaa gggacagcta
     1201 aaccaattga attttttaaa gctgattgat gaatagttgt ttgatattac aaaacaataa
     1261 tttcaccgaa ggtgaaacaa gcgaaacttt acaaaaagtg gctagcattt gctagccttt
     1321 tttgtattgt ggagcgtggg ttttgagggg gattcaaggg ggagcttgcg cccctttgaa
     1381 aagcggttat ttgcaccaaa tacgtaggta tttggctagc aaatatagag cttgccaaac
     1441 actttagtga gttttgtaga aagtaccttt aaaggtactt tcacattacg aacgatacgt
     1501 ccgtaggacg tactgaaagg gtgggattta tgagcgaact aatgcaaaat ttggctagca
     1561 aaaaaagtta aaaaatatat gctaataatc gagcgaaatg aacacttttt caagtgagcg
     1621 aatccgccat gacaaagctg aagcagttta tttcagcttt gtcatggcgt gtgcctagac
     1681 ttttaaaaaa gtcggcacac gagcaaatat ctttatcaag ctctaatttg cgttttaaga
     1741 cgtttgaaac gtctaagtat ataaatatat aagagtaggt ttaagagtgc ttaaatcgaa
     1801 ttttagaggg cattcagaca actttagggt tgttagggct tgccctgacc gcctgtaagg
     1861 cgctttaaga acaagataag ggatttatac taagactgtc ttaaaacgtc tcaaacgaag
     1921 gctcatagct tctcaaagag ttccttatga acatttttag caaaggagcc ggaagcgact
     1981 gcgcagtata atgtgagagc agagcgatca caccttttta agcgactgta gggagtaaga
     2041 ttcccagttg agaaaaatgc gcgaatctgt ttggagaaaa aagaagtggt tttttaggcg
     2101 ggaaattttg tccccctctt ttataatctc tttttaaacc tctttttaaa cctctttacc
     2161 gtcggctata tccttactct cccaagggaa tgacgctttt atctacatcg ttttgtctgt
     2221 ttatctacat cgttttgtct gtttatctac atcgttttgt ctgtttatct acatcgtttt
     2281 gtctgtttgt acattgaatt agtacatcga cgatgttaat ataagatgta gataaacagg
     2341 cattttgtat ttcataacgt gtatctcttg tgcgagtaag aaaggagcta ttttaaatat
     2401 gtctaatgaa atcgtaaagt atcatcatga attaaataca attccgttgc gaaaatttac
     2461 accagtcgaa atgaatttgt tcttttctat cgtatcaaga atgcgtgatg taggtgataa
     2521 aaaagtacaa tttacattcg agcaattaaa agatttaagc aattataaag caactgcaaa
     2581 tgttcgattc atagatgatt tagaaacaac gtatgacaaa ttaatggatt tacgttttgg
     2641 tagaagaagt gctgacggtt tgcaacgtga acgttttgtt ttatttaatc aatttaaaat
     2701 cgacggaaaa tctgatattc cttttgctga aatacaagtt catgaaaaag ccttaccctt
     2761 acttaataat cttgaagaat gggtgcgcta ttctctccaa caattcaatg aattagaaag
     2821 cagttattca aaaacaatgt ttcgattact aaaacaatac agaaccaaag gtttcgctta
     2881 tttttcaaaa gaagactttc atgaattact tgatattcct aaaagttaca accaaggtac
     2941 gattgatcaa cgtgtgttca atccaatacg acaagaacta accgcaatct ttaaaggttt
     3001 aacaatcaaa aagaaatacg gaaaaggacg gggaaagcct gtcattggct atcaatttac
     3061 atttaaacct gagataaaaa aatcagacga ctttaacaaa catgccccaa caaaagaaga
     3121 acaaaacaaa gcattagaac tagctaacca acaagttcaa tctgaaaaag cacctacttc
     3181 attacaagaa aaaatcaaag aacaaaagcg aaaattggaa cttttacaac agttagaaaa
     3241 gttagaaaga gaacaaaata cccaaaataa agaaaataat gcacaaaaat cagagataaa
     3301 ttccgatatt ccttcagaac taaaaaaaga cttattagac aatcttcaaa acttattcaa
     3361 agattgaggg gtttttagat tttatgacag aaaaaacaat caaagaactt gctgaagaat
     3421 taggagttag taaaacagca attaacaaaa aagtttctga cgaaaatcga aaactttggt
     3481 tttcgaaaat cggaaacaag tttgtgatta atgaaaccgg acaaaaggct ataaaatcaa
     3541 tgttttttaa cgaaaacgaa aactcaaatc aacaactagt tggcgaaaaa acgaaaaccg
     3601 aaaaccacaa aaacgccaac caagttcccg aattagaagt cctttctctc ctagaacaac
     3661 agttaaatca aaaagatatg caactacaag aaaaagacga gcaaataaaa caaatgcaaa
     3721 aattactcga ccaccaacaa caactcaccc tgcaagccaa ccaacaaatc gagaaactgc
     3781 aggaacaact acagttgact tatacggaag aatcttcgga aaataaatca acgactcttt
     3841 ctgaagaaac agaatcgata gaaaaacaat caaaggcgga gaaaaaatgg tggcaattct
     3901 ggcaataaaa ccagcaattt aacagaatta ggcagaagtc gcccatcctc tataggtggg
     3961 tgatgaatgc cgttcggtat gagggttacc gttggtaact cgaagaccga caactaaggc
     4021 gtattgcctt tttattttgt cccatgttac aatcagtcta tacaagaaat gagttgatat
     4081 aacatggaat tagatacaaa taatcattca gtatttcttc tttattacca ccttgtttta
     4141 gtaacgaaat atcgtagaca agtgattgat gatgaaatat ctgattatgc caaaactact
     4201 tttgaaagaa tttcagaatc gtatcacatt actttagtgg agtggaatca tgataaagac
     4261 cacgttcata ttatgttcaa agctcaacct aaaacagaat tgacaaaatt catcaatgcc
     4321 tataaaagtg ccagctcaag attgataaaa cgtgactttc caagagtcaa acaatttctt
     4381 tggaaagaaa tgttctggtc taaaagtttt tgtcttctga caactggtgg cgcaccaatt
     4441 gatgtgatta agaagtatat ccaaaatcaa ggaaacaatc ataaatagaa agtaggtgaa
     4501 ctttatggaa caactaaaag catacaaatt cagaatctat ccaacagagg aacaagaaat
     4561 cttctttgcc aaatcttttg gttgtgtccg taaggtctac aatctaatgc ttgatgaccg
     4621 aaaaaaggcg tatgaagaag ttaaaaatga ttcttctaaa aaaatgactt ttccgacacc
     4681 agctaaatac aagaaagaat ttccgttttt gaaagaaatt gatagccttg ctttagccaa
     4741 tgcccaactt aatttagata aagcatacaa gaattttttt cgggataaat ccgttggatt
     4801 tccacgtttc aaaagtaaga aaaatcctgt tcagagttat acaacgaata atcaaaatgg
     4861 tacagttgct ttgattgata gtaaattcat caaagttcct aaattgaagt cattagtaag
     4921 aattaagctt catagacaac caaaagggat tatcaaatct gctacaatat cacgtcattc
     4981 tagcggtaaa tactatattt ctttgttgtg taaagaagaa attatcgaat tacctaaaac
     5041 taattccaca attggtattg acttaggtat tatggacttt gcgattcttt ctgatggaca
     5101 aaaaattgat aataataaat tcacgtccaa aatggaaaag aaactaaagc gtgaacaacg
     5161 caaattgtct aggcgtgctt tattggctaa aaataaaggt accaatctat ttgaagctaa
     5221 aaattatcaa aaacaaaaac gcaaagtagc tagattgcat gaaaaagtaa tgaatcaacg
     5281 tactgatttt ctgaataagt tgagtacaga aatgatcaaa aatcacgata ttatctgtat
     5341 tgaagactta aacacaaaag gtatgttgcg aaatcataaa ttagctaaaa gtatttctga
     5401 tgtttcttgg tcaaagtttg tgactaaatt acaatacaag gctgactggt atggacgaaa
     5461 aatcatcaaa gtagataaat ggtttccgtc tagtcagatt tgttcagagt gcggacataa
     5521 agatggcaag aaatctcttg aaattcgaga atggacttgt cctgtttgtc atactcatca
     5581 tgatcgtgat atcaatgcta gtatcaatat tctgaccgaa ggtctaagac tacattctat
     5641 gggattggct tagatccaat catcaagaac cgtaggaact acggggatag cttggtaaat
     5701 aagagacacc gctgttagta aagaaatacg ctatcaagta tgctctattc ccaagaagct
     5761 cccacttcaa gcgttaagaa ccgtcaggtt tagctaagtg gtgagtagtt cacattaata
     5821 aaaattgctg gttttattct tctttttgct tttgcgagca gagcaacatg gacccatgtt
     5881 gcgatatttt ggtggcgccc caatccccgt tttttcgttg aatcttactc ggactgcact
     5941 cgttttacag ctgatttttc tgaattttga aagagcataa ggaaacgtgg tatcactttc
     6001 ttacgaaagg tgctagatag gctgtgccta tcacgtttct cagaaaaaaa tttgctatca
     6061 cttttttgcc attgtggcta ggctatgtat agtgttttgg cgaaaaatag ctatacaaat
     6121 actatatttg tatggtatac ataaattata cgctgatacg aaaggaaaat ttccatggac
     6181 attttagttc gaaatatcaa tcccaaaatt attgcaagaa tagataaacg ttgtcaggaa
     6241 ctcacaacca aaacgggcaa aaaatggagt cgaaataaat atctaaaagt tctaatcgaa
     6301 agtgattttg accgtcaact tatgaattac aaaaaagatc aattcgattt attactcgaa
     6361 aaatttattg ccattcaaac agaaaatacg aacatactga atgactatgt tcacacaaac
     6421 aaccaactca ttgaaacact tatcggagga atataacatg agaaaaactt ctgttcaaaa
     6481 acacgtaacc ctttatccca gtcacgttga cgttttagat tccatcatcg aaaaaaatcc
     6541 agacgtcaac aattattcag aagccattcg ttttttatgt atgagttatg aacctgatac
     6601 caatacaacc acaaccagta accaagcaaa actcaatgcc atgggaaaag aaatttctat
     6661 catgacagaa atcatggcag aatttgctga tttatatcta aaagatactg gtgttcttat
     6721 cggtacaaat tctaaagtgt atcaagaagt gaaagaaaaa attgaaaatc aactcaatcc
     6781 ttcccgacga aatcaaccca aaaatcgtca aaaagaaacc aatcaaacct ttacttctgg
     6841 aaattttgac ccaacaaact ttctaaaaag ataaaaaaag aaaccgatcc ctaaaaaaat
     6901 tcctcgattt ctttttaggg atcagtcttc cagcaagatt tgcaagttgg taccaacttg
     6961 cgaaaaaaaa gaaaaatccg cttatgcaac ttcttctttt tgtattttga ggcgctaccc
     7021 caatccccaa gtatcggtta tttaatagcg cggaaatcca aagaatagga cgtaccctga
     7081 aacaaaaaaa gactgctctc acgagcagcc caaaatgaga ttatcctatt catcaacata
     7141 aaaatattac aaagtgaaga actagagaaa gacaaaaaaa gcatatcaaa tcctctcaaa
     7201 aataacaagt ctttaaaaaa tgacagataa aaatgcacaa agaactaaaa aagagaaatc
     7261 gcgtaaaatc aagcattaga agctagtaac tagctagacc aattaaaaat cacaaagtga
     7321 gcgtctctat ccattggtag aggggctact tggcatagtg tacattatgt taactaacgc
     7381 tataaataac gattatgttc ccaaaacaat attttatgaa ctataacaaa aatttgttat
     7441 cgcatattgt atattttaat tataggtgta taccacttaa atttaacaca aaggcgtgat
     7501 tattttgaaa cccaaaaaga aaatttatct aggtctcctc ttttccttga tgattggaat
     7561 ttttagtata ggaattacaa atacctatgc ttcagaaaat tctaatttca atatcaactc
     7621 ccaaccaatt gaagaacaag caattaattt tgcgaaaact gattttgaac actccataac
     7681 ttcaattctt ttaatggaaa ataacaccga aactgatatt aactatactc ttggttcgcc
     7741 atttaaaatg ctcgatggag aaaatacggc aaatagttct attttctttc cagtattttc
     7801 agacgctgat aatacgatta aatatattta taccgtc
//
LOCUS       DACDIG010000101         7570 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.101, whole genome shotgun sequence.
ACCESSION   DACDIG010000101 DACDIG010000000
VERSION     DACDIG010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.101"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            25..1452
                     /locus_tag="IV247_002576"
     CDS             25..1452
                     /locus_tag="IV247_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP8778601.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1544..1909)
                     /locus_tag="IV247_002577"
     CDS             complement(1544..1909)
                     /locus_tag="IV247_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP8778602.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1906..2271)
                     /locus_tag="IV247_002578"
     CDS             complement(1906..2271)
                     /locus_tag="IV247_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP8778603.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2463..2711)
                     /locus_tag="IV247_002579"
     CDS             complement(2463..2711)
                     /locus_tag="IV247_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778604.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2710..2838
                     /locus_tag="IV247_002580"
     CDS             2710..2838
                     /locus_tag="IV247_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAP8778605.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2895..4589
                     /locus_tag="IV247_002581"
     CDS             2895..4589
                     /locus_tag="IV247_002581"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAP8778606.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4656..4862
                     /locus_tag="IV247_002582"
     CDS             4656..4862
                     /locus_tag="IV247_002582"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778607.1"
                     /translation="MFLIWLDLLPQMQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFL
                     TWLDLLPQMQAFEHRLPRGYEKGV"
     gene            5240..6373
                     /locus_tag="IV247_002583"
     CDS             5240..6373
                     /locus_tag="IV247_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAP8778608.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6404..6724
                     /locus_tag="IV247_002584"
     CDS             6404..6724
                     /locus_tag="IV247_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778609.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6753..7217)
                     /locus_tag="IV247_002585"
     CDS             complement(6753..7217)
                     /locus_tag="IV247_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8778610.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7349..>7570)
                     /locus_tag="IV247_002586"
     CDS             complement(7349..>7570)
                     /locus_tag="IV247_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8778611.1"
                     /translation="VSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAA
                     VNLVLSILIYLPFVKVATIQERRKEALDA"
BASE COUNT         2473 a         1461 c         1367 g         2269 t
ORIGIN      
        1 aaaatacaag gcggaggctc tactatgatt gaagaatata ttgagaaaaa tattttaaga
       61 cagctgtttt tatgtgggca attctatgta aataaagaag tcaatctaga aaagctctcc
      121 aatctcctgc atgtttgcaa aaccacttta ctcaatgata tcaataacat aaaaaaagaa
      181 tttgaagaac agatcgctta tacacatcgg gaaaaggacc gttacacttt atatttttca
      241 gaacatatac ctcgatgcaa gatcatgcag caattgtctc aaaattcgtt gtttttaaaa
      301 acttgtctgc tttatttgga agaagatgaa cctgattatc ttcaattaac tgaatgtgaa
      361 ttcatctctg tcagcaaagc ttattcatta aaaaaacaag tattagctta ttttaatgac
      421 tgcgggatcg aaatcgaccg ctactcccct cgattcactg aaatggaaag acgtctgctg
      481 cttcttaatg tgtcgtatcg tttaggcggt tttaattctt gggagctacc tgaatcgttt
      541 tttgaacgtg ccgatcgttt cattgaatcc gtaacggaaa atagtggcag attttatgat
      601 aaagaaaata aagaaatctt atcgattggt tttgctatca gtttcctacg ccagcaagtg
      661 tgtgctgtga caatagattc aaaatttatc gaagaaatca aaaaacgtcc tatttacaat
      721 tatgtcgaat cagcatggga aaacactgat ttccaaacct attataaaaa agaagaattc
      781 gcctttattt tgacactatt caatctttgt aattatggtt tccattctta tcagttgatt
      841 gcggaagact ttcaacagct acaccaagtg tttattgaca atactcccga aataaaagaa
      901 ctagttgcta cctttgaatc tcacttcaat caagaattat tcggaaacca accgttcgaa
      961 cgtgcgctga ttcatctgat gcgttcggct tgggataact atcaattatt catgccagaa
     1021 aaattttatc ttttaaatga ggaacagaca aatttatata aagaagtcca gacaattttt
     1081 tccagctggt ccagccagtt accttatgat ctacggttga atcctaattg catgcgggct
     1141 tttgtgatcg aattgagcgg aattctccgt ctgacaaaag aacatctgac tatctatatc
     1201 gtcacaaatt cggatgttca ttatctgatt tatcgggaag ccttggaagc tgtaactact
     1261 ttcgattttc aagttgcacc aacaatttat tcaagtatat ctgatataaa gaagtatgcg
     1321 cagcagtcct caaaccgggt attatgtgaa cgtacattat atacaccaga tgcggtccaa
     1381 tatgaaaata ttattcctat ttcgatcaat acgatcgatc gagctatcat ttcagctgtg
     1441 caaaacaaat aaggggctgt gacaaaaatc atgtcacagc ctcttatttg ttgaagatgc
     1501 ccatttctgt aacaatctct tgatacacgt caggctcaca acctcactta ttttggacat
     1561 ctacgggtcc gatctcttta tttaaaaaat aagaaatgat ggttcggata actaccaacg
     1621 ccgctagttg gaaaatatcc tgcaaggtag gtttgataat cgattcgatg atcccagctg
     1681 aaacaagaat ttccaaactt agaagtatat agcctcccaa catcttttta atagcattgt
     1741 tctcgctgac tcggtcgctt tgcacttttg tatggacaaa taaatatttc aaaaaatcct
     1801 tgattgatag acaaacaccc caaaccaata aacagatagc aaagatatca agtcccagta
     1861 caagtttttc gaaatatgga agaagaaact gcatcaactc tgtcgtcatc ctacctctcc
     1921 tcttctttgt ttttctttgt cttctatttc ctgattcaag aaataagaaa tgactgttcg
     1981 aatgataata ataagtgcaa gcttcaaaat atctgtccaa gtcggcttga taactgactc
     2041 gatgatatca gcaacgacta gtacttccaa gcttaacaaa atataacttg ctagcagttt
     2101 tcttgtatct gcattacgag tattacgttc tgcatagttt ttcttgacat gagaccaatc
     2161 aaaaagtttg aatagaacaa gaacgatccc gacaattaag ataccgatag aacaaatatt
     2221 taaaatagca atgatccatt ctagaaattc aattaaattt gagcgaaaca tatacgctac
     2281 ctactttctt ttaatttgtt cctattcttt tattgtaaaa gaaagttacc ttgttcgtct
     2341 atttaaaagt tgttctaatg ataacggttc gcttaaattt ttctttttca ctctcccatt
     2401 tttctccctc catccataaa tcgtggaagt atccaaaaca acgtagcaag ttaaaataag
     2461 gcttagtccg tcatcagctt ttcaaagaag cactgtctcg gtgctttttt tgtacccaaa
     2521 tttctaaaaa tagaaccata cacctcctca aatcttttct tcttacctac cctattttta
     2581 tttacaggaa aatcagacac ttcttctttt ttgtcaaaat ttcctttttc ttctcgtcct
     2641 atcatttggc attctaataa aaacgaacta tccttggata tagaaagaga aaacaaggag
     2701 gcgtttttca tgagtaaaaa attaatcaaa gtcggcatcg gtttaggttt gcttgccctt
     2761 ggcgctgcat atctaggaaa aaagacagga ctattcgaag atgacagtca tttatatgat
     2821 gaattcgaat caatctagca tcctatttag ttgaatcact acggacatac ttggaagaca
     2881 ttggaggaat cacaatgaga aaaagtaaag caataatgat tggtgccggt attgccaata
     2941 tggcagcggc tgtttatttg attcaagaag gcaaatggaa aggggatcag atcacctttt
     3001 attcattgga tgatcacggt tcaaatgatg gtgcaccgac cgacactgta gtggatgaat
     3061 attggaataa aaaccatcca ttagaaaacc aaaaaggcta tattgctcga ggcggtcgca
     3121 tgcttaatta ccgtacctat gtagatttga tggatctgtt aagtcgaata ccttctgtta
     3181 ctgagccagg gatgactgct gaagaagata cgagagattt tgatgcaaaa caccgaacct
     3241 ttgacaaagc acgtttgatg gaaggtggca aaggaatcat tgatggcgga aaactgggat
     3301 tcaataataa agaccgtatt cttctgacta aactgattgc tatgcctgat tcagaagaag
     3361 aaaaactaga caatatcact atcgaagaat atttcaaaga tgatccacac ttttttgaaa
     3421 caaacttctg gtttatgtgg gaaacaacat ttgctttcag aacacgtagt tctgctcaag
     3481 aattgcgtcg ctatatgcat caaatgattt atgaattcac tcaaatcgaa catctagtgg
     3541 gcgtcaatcg aacacgttac aatcaatacg aaagtattat gcttcctttg atcgagtact
     3601 tgaaagaaca aggatgcaaa atcattttga accgccgtgt cactgactgg gaattcaaag
     3661 aaacaccgat gcaagatgaa atcactgtga ctggtttgaa aattaccaat acactgactc
     3721 aagaagaaga atacgttgtg gtcgatgaag atacagcagt tattttcact aatgggtcca
     3781 ttacagattc agctacttta ggagattacg aaacaccagc tgtagaaaat atggattatg
     3841 gtgcagcttc tagtctctgg aaaaaagcca gtgaacgttt ctacaacttg ggaaatcctg
     3901 acaaattctt tgctgatcga gatgcaagcg aatgggtcag ctttaccctg acgacgaaaa
     3961 accatttgtt gttgaatgaa atcacccgta tcacgactca agaaccagga aatgccttga
     4021 attcatttat ttcaaccaca ccgatcacgc cgttaggcca aaaagatgtc aatatgtcta
     4081 ttgtcgtgca tcatcaaccg cattttacgt ctcagaaacc aaatgaaaca gttatttggg
     4141 gttatttcct ttatccaaga cgacgtggcg aatttgtgga taaacagtat attaaaatga
     4201 atggaaaaga aatgctagaa gaactgattg gacaactttc aaaagtagat cctggaccag
     4261 tgaatattcg tgaaaaagaa acagaaattt tcgacagcgt aataaataat atccctgtct
     4321 atatgcctta tgcttccgcg ttgttcaata atcgtgccaa aagcgatcga ccaaaagtca
     4381 ttcctaaaca ttcgaccaat ctggccttta caggagaatt tgtcgagcag ccttatcaga
     4441 tgatctttac tgaacaaagt gctgtacgtt ctggagaaat cgctgctttc cattttgcgg
     4501 gtattcctat gtcccgtctt gtcaaaacac cgcgttacga caaagatatt cctactctag
     4561 ctcgtgccgc aaaaaaaatg tttgaataat caagaggttg tgaaaaagct gccctgcatc
     4621 aagtaataag aacaaccagg ctaaaacagc ttcttatgtt tttgatttgg ttggacttat
     4681 taccgcagat gcaagctttt gaacaccgtt taccaagagg ttatgaaaaa gctgccctgc
     4741 atcaagtaat aagagcagcc aagctaaaac agcttcttat gtttttgact tggttagact
     4801 tattaccgca gatgcaagct tttgaacacc gtttaccaag aggttatgaa aaaggcgttt
     4861 agtttcgagt atgaagaaaa attttgtgaa aatggttcct cacgtttttg cataaatttg
     4921 acttaatacc gagaaactga tcccttctgt cataacctct ttttatttat gtctgtttct
     4981 ttttgaaaac aactattttt tcaacgttta ctctcttgcc aatccaacat catctaacgg
     5041 atacaactat ttcaatgatt accattacac taaaaaatcg gaaacacacc tctccgaaca
     5101 caaatacata agactataga atgtttcatt taagaaaaaa agcgtttcat tatcaaaaag
     5161 gtaagatctc ctttaaaatg aaattatctg tagtactaat aatgcagaca attttatgaa
     5221 tctgaaagga gcatctccaa tgaaagcagt cacttggcaa ggaaaacaga aaatggaaat
     5281 cagaaacgta gatgatccaa aaatattaga accaacagat gcaatcatcc gtatcacagc
     5341 aacagccatt tgcggctccg atctgcatct ttaccatcat ggtgaagcag ttttggaaaa
     5401 agggtatgtt gtaggacacg aaccaatggg aatcgttgaa gaagtgggat ctcaagttca
     5461 aacattgaaa aaagggcaac gtgtggttat cccttttaat attagttgtg gtcattgtca
     5521 tttctgcctg aatcacatgg aaagccaatg tgatgaatca aatccagatc agttatatgg
     5581 cgggcttttc ggatttggca agttgaacgg taatcattgg ggtggtcaag cggaatatct
     5641 aagagttcct tatgcagact ctacttcatt tgttgtacca gataatgatc ttcctgatga
     5701 aaaagtttta tttctatcag atattttgcc cacagcttat tggagcgttg aaaacgcagg
     5761 agtaaaaaaa ggcgatacag ttgtgattct aggttctggt ccagtcggtc tattcgctca
     5821 aaaattcgct gttatggctg gagcagagcg cgtaatcgca gtagatccag tacagcaccg
     5881 tctagataaa gcggctaatt acaatggggt tgaaacttat ttcttagaag acacggcaca
     5941 agctggaaca gaattgtatg aactggctaa aggtggcgca gatgtcatta tcgattgtgt
     6001 aggaatggac ggattagagc ctgtcaaaga aaaagcgaaa aatttagtaa gcttacaaag
     6061 cggcacaatc tctccattac agatggcatc gcaagctgtc aaaaaatttg gaacagtcca
     6121 agtaactggt gtttatatga ctccagcttc ttcttacccg ctgcaagaat tttttatgcg
     6181 ggatatcgct gtcaagcatg gacaggcacc aattatccat ctaatgccaa aaatttacgc
     6241 aatgattgca gaaggactat tcgatcctac acaaatcatt actcattcgc tgccactatc
     6301 agaagcagca caagcatatg agatctttga caaaaaagaa gataaaaata taaaagttgt
     6361 tttgaaacct tgaattcagt aaaaaataca ggtgattttc tttatgccca aaatcaaacg
     6421 aaatacccat gagatcgaaa taggaaaaga agaagatatc gaaatacgag gacgtcgctt
     6481 ccgcttctat ttccaaaatc gttacacatt gatctcactg gctgtcgatt tattaaccgg
     6541 gattttctat atttttggaa gccttgcttc cttaactaaa atccccgatc gatatggcac
     6601 ctatttttat ttggctggtg ctattttctt gacgattcgt cctattctca gaatcgtaag
     6661 gaacattttt atttacgacg aaaaaaaagc aatgaagaga acaagcaaaa aaaaagaggc
     6721 atgagaattt ttcaaatctc atgcctcttt tttcacccta tcgtatcact ttgatacaga
     6781 cgagcggtcg tcccactttc ttccttgacc attgtcaaaa atgtccactc gtatcgttca
     6841 taaaacgtgg tatgatctgt aattaagtaa agtttttcgt acccaagttg atgcatttct
     6901 tttctgattt ccgctaatat ccgcttggct atcccctgct tacgcttttc cggttcaaca
     6961 aacagggcac aaagatttgg tgtcagatca gggcggttat ggaagtcgtt ttcaatgacg
     7021 cccgctcctc cgacgatggt ttctttttca tctaagacca cataccattg aggaatactt
     7081 tccttattag cgattccagt acgaatactt tcctcataca caacagcagg aactccccat
     7141 tttgatgaaa accaattcgc gctttgtttt actaattctg ggtgttctct taatttgatc
     7201 cagttatatg taaccaatgt ttaaacctcc gactaaaata aaacgtttac aaaaattata
     7261 caagtcttta tgaaaattag caatgacaac aatagtgatg taaaaaaaaa aacggaaaac
     7321 aatcacttgt tctccgtttt ttgattaatc aagcatctaa agcttctttt cttctttctt
     7381 ggatcgtagc taccttgacg aacggtagat agatcaaaat agaaagaacc aaattgacag
     7441 cggctagtac accacctgca atactttttg ttgccaatac accgccaata attggtggag
     7501 ttacccatgg aggcataacg gtagctgctg gcactaaacc tgtagaagtc gcaaagtaag
     7561 caacactaac
//
LOCUS       DACDIG010000102         7537 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.102, whole genome shotgun sequence.
ACCESSION   DACDIG010000102 DACDIG010000000
VERSION     DACDIG010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.102"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            107..1248
                     /locus_tag="IV247_002587"
     CDS             join(107..335,335..1248)
                     /locus_tag="IV247_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8778612.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
     gene            1483..1935
                     /locus_tag="IV247_002588"
     CDS             1483..1935
                     /locus_tag="IV247_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778613.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            1949..2638
                     /locus_tag="IV247_002589"
     CDS             1949..2638
                     /locus_tag="IV247_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778614.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            2763..5114
                     /locus_tag="IV247_002590"
     CDS             2763..5114
                     /locus_tag="IV247_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAP8778615.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            5194..5589
                     /locus_tag="IV247_002591"
     CDS             5194..5589
                     /locus_tag="IV247_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778616.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            5914..6531
                     /locus_tag="IV247_002592"
     CDS             5914..6531
                     /locus_tag="IV247_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8778617.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            6572..6871
                     /locus_tag="IV247_002593"
     CDS             6572..6871
                     /locus_tag="IV247_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778618.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            6905..7072
                     /locus_tag="IV247_002594"
     CDS             6905..7072
                     /locus_tag="IV247_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8778619.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(7117..>7537)
                     /locus_tag="IV247_002595"
     CDS             complement(7117..>7537)
                     /locus_tag="IV247_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8778620.1"
                     /translation="SLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLD
                     TAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLV
                     FKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2594 a         1138 c         1502 g         2303 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc gtagaatcta actactaaga aaaggacgtg tccacgatgg caaaagccaa
      121 gaaaaaattt gatgaagact ttaagaagat gatcttagat ttaaaccagt ctggccaatc
      181 ggtcgaagaa ctggcagcgc aatatggtat cgctacacaa acgatttatc gttggaagaa
      241 gcttcataca aagaatgtag caaccggcat gactgaggca gaaatcctag cgatgaaaaa
      301 ggaaatggcc cgcatgcagg aggaaaacac tatcctaaaa aaggctttaa ccatattcgc
      361 tcaaaagtaa aaatgagtga tgtcttcgaa tttattcaat caaatgttca tgatcatgac
      421 gtaaaacaaa tgtgtaccgt tttggaagca ccgaaatcca gctactatga ctggaagaaa
      481 aagaaacctt ccaagcgcca atcagaaaac aaacgcttaa accgcttgat ttcaggaatc
      541 tactttgaaa acaagggtat ctatggtgcg cctaaaattc ataagattct tgttggccgt
      601 ggcgagacct tgtccttaaa gaaagtacaa atactgatga gaaaattagg cttgcgttca
      661 gtcaccctca agaagtacaa gcctggcaaa caagcgaaag ttaaaagtga aggacgtaaa
      721 aacctgttaa accaagattt taccactacg tcgatcaatc aaaaatgggt gacagatatt
      781 acctatattc ataccctaaa agatggctgg acctacctct ccactattca agacctccac
      841 acaaagaaag tcatcggttg gaagtttggc aagcagatga cgaaagaatt ggttatcgaa
      901 acccttgaca atgcgctctt aaaccaaaca ccctccgaga acttgatcat ccactcagat
      961 ttgggttctc agtacactag cgaagcctat gaggcaaagc tgaatgaatt gaacattcgg
     1021 cactccttca gtcgtaaggg ctgtccctac gataatgccg gtatcgaatc gtttcacgcc
     1081 tcactcaaaa aagaagaagt ctatccatca aaagcgtatg aaaactttga agctgcttat
     1141 ttagcgttat ttcaatacat tgaaggattt tacaatcgca agcgaatcca tagtagtatc
     1201 aactatttaa caccaaatca gatggaggag ttggcattgc aagcttagtt cctagctacc
     1261 acttacattt ttttggcgag atttccacag ggtttcaact tcatcgcttc gatcagcggt
     1321 caaacggttt tggtttgacc gctggtcgaa acgatgagat actcacccgc tggaatctcg
     1381 aaaaaattct cacaaatcgt gtccgaggta ttgactcaaa tccaccaatt atcttatcgt
     1441 agatatatat tataaattta ataagaaagc gaggtgagaa tcgtgaaaaa aacgatacta
     1501 agtatttttt cagtatttac aatattaatt gggggctatt ttcttttgaa tttagattct
     1561 gttaattcaa acgctcaaat aaaagatcaa agagtagctg aacataataa taaaaaaatg
     1621 agtttgacca aaaatatcta ttctatgtca gatgaagaat tgtataaaga atttggtgat
     1681 ttatccgggg aacagttaga agaaagtatt ccaggaatta gtgactcaga taaaattgtt
     1741 gtcttgtctg ctggtgggca attaatggga gatggcaaat ccgaattagt tagttataaa
     1801 caaaatcaca gacagtttat tgatgttaat actgattcca acgctatttc gattaaggat
     1861 ctaaaagagc ttttaaatag taaaagaaat gaattaaagc agataaaaga aactaattca
     1921 gtggaattag agtaaaggaa gattcattat gaaaaagatt tttggtgttt ctgttgggct
     1981 acttatgatg ttaacatttg gaaatgcagt atatgctgct gagaaagaag ttatttcaga
     2041 tccagagcag tacgcctcac gatactttgc tgattttgaa gaagaagata aagtacttgc
     2101 attgccagaa ggtggttttt taaaagggga agctaaaatt gtatctatgg ataatccaga
     2161 tataattcaa ggttcatata attcagaaac tgatcctgct tcagtttcag tgacagaagc
     2221 aaaaaaaatt gttgctgaaa aattcaaaaa tgatattgaa actactccaa ttatgcctcg
     2281 aggagcattt ccacccgatg ttggtactag cggacacctt cttgtactag caggaggagg
     2341 aagctataaa tcacagccat ttagtggtgc gggatggcgt tttggaggta tgggtgtaaa
     2401 accagctgat ggtacaggca actttttacg ttggaaatcc tacattgacg atggaagagt
     2461 aggaaatgta aatcaagcca acgcaactaa atctggttct attcaaggaa tggcattgta
     2521 tgctcgtgaa ggattcaaat actttgcacc aaacgggcct ggttggacaa tgaatatagg
     2581 tatttattac acttataatc ctgttgcagg tacgtattat gttgttcaaa atatttaata
     2641 aaacagcaca aagagatttc tttgtgctgt tttattattt ttagttgttg acaaacacta
     2701 aaatataaaa tataatcaac acataaacga acgtatgttc ggcaagaaag caggggattt
     2761 ttatggatga gatggtttta agtactcaga aatggctaaa caagaaatac agtaatgtta
     2821 cagggtttga taaggttcca gaaaatgggc gaacagggtg gcctacaatc tatggactta
     2881 ttgaaggact acaagtagaa cttggtatta ctaatctagt agctaatttt ggacctacca
     2941 ctgaaaaaat gtacgataat caggttactc ctaaatgggg caaaaatcta cctaaaaata
     3001 ttgtatttct tattcaaggt gccttttggt gtaaggggat taatccaggt ggcttcgatg
     3061 gggtatatac tccatattta gatactgctg taaaagagtt gcagactgat gcagggttta
     3121 atggaagcgc tgtaactgga gttttggatg cgaaatgggc caaagcgtta tttgatatga
     3181 gtgcttttgt tcttgttccc ggcggagata gtaaaattag aaaaatgcag caatggttaa
     3241 atgtaaatta tttagaatac acgggaataa tgccttgtga tggtatttat caaagaaatt
     3301 caaatcaagc attaattttt gcattacaag ctgaactagg atattctccc agtgaagcaa
     3361 cagggtctta tggtaatggg acaactagta aaacttatcc tgtgtctgaa ggtaactcgg
     3421 gaaactatgt aagaataatt caatatggat tatatgttaa tggttatttt gaagacggta
     3481 cattcgatgg tattttcact aaatatatgg gtttagaagt actggcattc cgaaaattta
     3541 tggtattgcc tgaatatact gaaatagcag atgttgttgt aataaaagga ctattgtcta
     3601 gcgctggaga tattagaaga tctgcagatg gcgcagatac ttctacgcaa ttaactagat
     3661 ctcaaatcca aacattggtt gataatgata taaaaatagt tggtagatat cttactggta
     3721 ctgttggaat tggaaaagat gaacgaaata agtatctaac tacagaggaa ttgaataata
     3781 ttttttctaa aaatttatct gtgtttccta tctatcaaga tggaggtgca gcattagctt
     3841 attttactta tgatcgtggc ttatcagatg gtaagaaggc tatagatgca gcaaaaaatc
     3901 taaatatacc attaacaaca gttatttatt ttgctgttga tctagacatg ttaggggaag
     3961 aaattcttgc atatgctgga gaatatttta agggggtatc tgctgtaatg tcatatagcg
     4021 ggtatcaaac gggtgtttac ggaacaagaa atgtttgttc tcaaataatc aataacgggt
     4081 atgcttcgtt tgcatttgtt tcagatatgt cgactggtta tagtgggaat ttaggttttc
     4141 ctatgcctag acaatggtct tttgatcaat ttgttgaatt taccatagga agtggcaacg
     4201 gagctgttgg tatcgattta atcgctacat cgggtagaga tagcggattt aatgagttat
     4261 caaatgatac taataatgat aattacattg ccaaatacaa tcagaaggtt ataaatcaga
     4321 tggtacgtgc gtatcaatat ctatcacaag ctggtcttga taatccttgg aaccctcatt
     4381 tgacatttta tagatatgta aattattctg gtttgtcatg ggacatcatt tcttctcctg
     4441 taacagagca tgatagaaaa atttatgatg aatataggaa taaattaact aatagcgagg
     4501 gcctgtataa ttattttata gatcctaata ctggttcaat aataggtttg cctcacttaa
     4561 ttgtgacgtt acaatcacag atgtttctag ccgacacaat caatgatagt gtttctgact
     4621 ttgcaggttg gttaggagat ttaatgactt gctggggtga agttaagaaa ttaggaatac
     4681 aaatggagca aggtgtattt gcattagtag gtacggctgc aggtggaaca tttagtctag
     4741 aagatttata ccaagatgtt gacgcggtaa atgtttatac cttagcaaaa agaaattctg
     4801 attcaacagc tttatcggct ttaaaaacgt actattgtga taagggtgct attgatagat
     4861 ttaatcaatt tgttaagaac agatttggtt cttcagataa aatcgcttca gtaacaaata
     4921 gtttattaga aaatgacgaa acagttgata tcttaggtgt agggataatt tcatttttca
     4981 ttgataaaat agttaataat tcacttggga cagattttac ttatcttgat tttattggag
     5041 atgaggggaa agttgcaaat ggattttctg aaaaaattat tcaatttcaa gaaaatagaa
     5101 gtaaacttaa ttaagaatcg tttactattt aaacagtgtc cttttccttt ttaaatatat
     5161 acgataaaat aagtatatga attgtaaaag gacatgatga ttatggaaga aatagaatgg
     5221 aataatctga ctcaaaataa taaaaataat tctggatgtg gaattatctt tatgtttgtt
     5281 atagttttgg gaatagtagt acttttaata actgtcaaga tagtagatta ctttgcgaat
     5341 actcctgaag aacaaataca gagaaaactt cctgagtttg aaagagtacc accttatgat
     5401 ccaagagata attcatttca agtaaaaaaa ttaaaagagt ctccaaatga aatgtggttt
     5461 gaagttacag ttgaatcaag cgacttcaaa gaaaaaaaga aagaatacaa ttatattgta
     5521 aaggcaaaag tatcaaaaag atttattttt aaccctaaat tagaaatata tagtgttgta
     5581 atagaataat agtgttaaaa aaattgtaaa agttaaagaa gtcaaattct gatgtgaaaa
     5641 ataaaatata ccatggaaga cataaaagaa aaactggata actagtttat ttaatggcag
     5701 accgttaagg cctgccattt ttatgtattt tagcggaata aatataatca tgcgggagtg
     5761 taaacttact gtttcaaaaa gtatactttt tgacatgcct tgattttctt tgtttatgta
     5821 tggcataata accacataat acaatttgaa acgtttctga tttaatctga ttgttttgaa
     5881 acaattttag tgaaaaaagg gtagataaaa acgatgagaa aattttttta tatccgggtc
     5941 agtagttatg atcaatcgat cgaacgacaa ctgatttccg cgaaggaatt aggaatccct
     6001 gaagaatata tatttgtaga aaaagccagc ggcaaagatt ttaaacgccc tgaatatcaa
     6061 ttaatgaaac ggatgtttcg aaaaggagat ttactctatc tacaatcatt agatagacta
     6121 ggcagaaata aacaaatgat tttagaagaa tggcgagaat taactaagga aaagcagatt
     6181 gatatttctg tgttagacat gcctctgtta gatactgcta gttacaagca actagatggg
     6241 ttagagacgc ttatctctga cttaattctg caattactca gctatatggc cgaagacgag
     6301 cggaaacgaa ttaatagccg gcaaagagaa ggcgtagaag ctgcgaaaca aaagggtgta
     6361 aaattcggca gaaagaaaat tgctttacct cctgaatttc ctgcgatcta ttctgactgg
     6421 aaagcaggaa agataactgc agtctatgct atggatcaat tgggcatgaa acgaaatacc
     6481 ttttatcgac gggtgaaaga atatgaacat accttgcacc acttttcttg atagatataa
     6541 cagctattag acgggaaggg agagtttgaa aatggactta acgaatcaaa gggtcgtttt
     6601 tactggaacg ctgcagcaaa tgacacgaac ccaagccatc ggtttggttc actctttaaa
     6661 cggccattgt caatcaagtg ttacttcaaa aacaaatttc ctggtttgtg gtcagtattc
     6721 taagtcgttg tttgatgatt ctctctatac aaaaaaggaa caaaccgcgc gggacttgat
     6781 tgcgttaggt catgagatta ccattttatc agaagtcgaa ttttatgcgt taattagtca
     6841 acagttgaaa gaatggcaac ggtatttata atcgttagta aaatttacaa ggaggaaaat
     6901 ttttatgaag cgaaaatctt atgatcgttg gggcattgaa ataactagtt ttaatcgaaa
     6961 tattgctttt cacgtggagt gtgactgtgg ggaattagca aaaagtgttt atcaaaaaaa
     7021 ttacttttac tgtcctaaat gccatcgccg atacggggta catggaacat agaaacgaaa
     7081 tagtgcggta gaaaagcgaa tgaatgtagt atatttctac aaatcattcg ctttctttat
     7141 tttataaata gttggtcgag agatcccgtt ttcttttgca atctctgcaa ctggaatgcc
     7201 ttgctttaac atgtccacca cggttttaaa aactaagcgt ttttgcggat ctgttgagtt
     7261 aatagaatac tcgattggtc gtcccttata tcggccggct ttttgagcca ataatactcc
     7321 ttgccgctga cgttcacgaa ttttctcacg ctcgttttgt gcaatataac ttagcagtga
     7381 taaaaacata tcgaacatag cggtatccag gagttcattt ccagtattaa aatctaaaaa
     7441 ctgtgcatct aatattttta acgctacttt tttctgtttc ataaaagcaa ctgtattttt
     7501 tatatcttca taatctcttc ctaaacgatc tagtgat
//
LOCUS       DACDIG010000103         7397 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.103, whole genome shotgun sequence.
ACCESSION   DACDIG010000103 DACDIG010000000
VERSION     DACDIG010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.103"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..142)
                     /locus_tag="IV247_002596"
     CDS             complement(<1..142)
                     /locus_tag="IV247_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8778621.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQ"
     gene            complement(183..437)
                     /locus_tag="IV247_002597"
     CDS             complement(183..437)
                     /locus_tag="IV247_002597"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8778622.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQKN"
     gene            complement(548..802)
                     /locus_tag="IV247_002598"
     CDS             complement(548..802)
                     /locus_tag="IV247_002598"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8778623.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(995..1270)
                     /locus_tag="IV247_002599"
     CDS             complement(995..1270)
                     /locus_tag="IV247_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HAP8778624.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1242..2195)
                     /locus_tag="IV247_002600"
     CDS             complement(1242..2195)
                     /locus_tag="IV247_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP8778625.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2807..4300
                     /locus_tag="IV247_002601"
     CDS             2807..4300
                     /locus_tag="IV247_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAP8778626.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4435..4731
                     /locus_tag="IV247_002602"
     CDS             4435..4731
                     /locus_tag="IV247_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP8778627.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            complement(4755..5714)
                     /locus_tag="IV247_002603"
     CDS             complement(4755..5714)
                     /locus_tag="IV247_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8778628.1"
                     /translation="MTYTHLTTDELVMIESYFKINQSVAKTAHFLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIR
                     CSARTIYRMFKKGLFNPSDLPMKGKRKPNGHQERRGKQAFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIENKLNHWFESVPKNLFK
                     SITFDCGKEFSNWKQISNANDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDESFIQSVASKRNNIPRKSLNYRTPLEVFLSYVSIDDLSNLI"
     gene            5897..6748
                     /locus_tag="IV247_002604"
     CDS             5897..6748
                     /locus_tag="IV247_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778629.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            6769..6981
                     /locus_tag="IV247_002605"
     CDS             6769..6981
                     /locus_tag="IV247_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778630.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         2442 a         1199 c         1330 g         2426 t
ORIGIN      
        1 attgctttac ccatttgtca aatgctgaag ccgttagttc atattctcga atgatttcta
       61 cacgtggctt tccagctaag taaagattga cgatttgttg cttgaattct tgtgaaaaag
      121 ttcttcgtgt tctcttagac ataaaaattc ctcctggtat gttttcttct agtctacaca
      181 ccttaatttt tctgtctagt taattgtagc ctatccatga tcggtttatt agacctttga
      241 ggtctatatt ttttaactac aatcgacctt aaatttaatt gtttcattag cttttggaca
      301 tgtttaagag atactgaaat cccttctttt aataaaactt gatggatctt tgtggcacca
      361 tagcgttgtt tattcgtaaa aaaaattgtt gaaatcttct ttttcaactt attatttctg
      421 atttctgtag ctgtcaacgg tttattctta taaaaataat agacacttct agggatttct
      481 aataaccgac acaacaaaga aacagtgtaa tccttacacc atttttcaat aaattgaaca
      541 agcgaattta ggttttccga gagaatatgg ctgcggcttt ttttaagatt tcaagttcct
      601 catttaaacg agcattttct tttctcagtt cagctacttc ttttccagtc agtcctgtgg
      661 actgatcagg taaatataaa ttcttccatt tgtaaatcgt tgcttcagat aagccatatt
      721 ctttcgctaa ccctcgaaca gaacgtccag tttgattcag ttcaacaatc atttgtttaa
      781 aattttcttc atatcgggtc ataaaaattc atcctctcgt agataagttt aatttattct
      841 tgtctctacg aaaagctgtc taagatttga tactaacacc aaatgttgta tacccccaaa
      901 caatacacat attttgaagg attaggatta ataaagctaa aaatatagta agtatactaa
      961 atatatttac tatatctagt atacttacta tattttaatc ttttgatcgt ctattatata
     1021 taatccgttc ctcgtcgttt aaagtatcaa cataggcgtc cattgcaagt ttgactattt
     1081 catacatagg aatatctttt gcataactaa gatcccgaat ggtttttagt acgggaggat
     1141 ctacttgaac tggttttctt tctttcgcag tgtactttcg ttcccctaac tcgacattaa
     1201 tatcatcttt aattgaaata tcttcagcac caattttctt cttatctttg ttcgttggta
     1261 ataatgccat ttagtttaat ctcctttcca agaggtagga tatgattggc cattgagtca
     1321 gcatatttag gtgtataatc aaatccttca atatcaccat gttgttcata gtattcaatt
     1381 ctttcttcta attctgtaaa gatgtctgca aaaatagccc accatcttcg atctacataa
     1441 tcatttaatt ggataccatt ctcaccatac atttctaacc gatccgagcc tttaacaatg
     1501 cttttaaaaa tgtttgattc accatactta tcaattgttt tttgatagtt tgcgtgttga
     1561 gaggaacgtc gcactgagaa aagaacaggc aaaattccaa tgatttgaag atgagactct
     1621 tcaaaattcg tgacaatcgg aactaaaact ttattaataa aatcttctgt tccttccatt
     1681 gcgtaccgtt ttaattcttg cattgcaata atataatctg cagcagttaa gaatgcacga
     1741 accacattat ctgttgatgg tgggcagtcg ataaagatat aatcgaaatt ttttctcaat
     1801 tcttctaaag gtttaatgaa ggccaatgat ctcttacgac gatcaggata tttttcggac
     1861 aagtattcat taagttcttc agatccagga gagccagcca taatatataa atgtggactt
     1921 acttgagtaa tacctgaaac ccagtcccct tttttgattg ctgcagacat ggatcgatca
     1981 aagctagtaa tttgatacgt tgatccaatg ttggaagtag tatttctttg ttggtcataa
     2041 tcaattagca acacgtcttt ttcaaaaagt tgactagaaa caatcgctaa taaatcattg
     2101 tctgtagtct taccaatgcc gccttttcta cctgtaagca ttacaacttt tcctagttta
     2161 tctgatgttt taaatgcagt ccgtaagttt tccatagttc cctccaattt cctatactaa
     2221 atatatttag tatacttcat atacttaata tattcgatat atctaatata ctccaaattg
     2281 ataagaattc agactttata aggaatatag tggatatact aaatatattt gctatattca
     2341 gtatatcaga tatattcaaa atgtcaaaat atatttgtgc gttttgcgtt gacaatttag
     2401 agtgaaaaga ggtaaactta attctataga aaccacagat aattcactat ttacaaagta
     2461 gcaaaagcag tggtactctt taaacagaaa aattacataa aaaaaagaga cggaaataat
     2521 tgattttagc agaatcaatt atttctgaat cacatggtta ggacatgtgt tcacgctcgt
     2581 ctcaagaata tgtaagtagc taccaactac ttatattaaa agttgatcgg ataccttttc
     2641 cgattgcctt tattctacca aaaaaaggcg agcaactcaa cagaagaacg tttatttagt
     2701 gtacttaaat taaatattct taaacactga agagagaaca catactatgg ctgagattaa
     2761 agctaaactg tgttctcttt ttgtttgaga aaggacgtta caatcgatgt tgagcgaaat
     2821 ttattcagct attcttaaaa acggcttacg tacatacaaa tatcgaaaca gcaccatgaa
     2881 acctgcaggt catattgatt ccagtaggag tggtgctatc tttgcgtacc gtagcaagca
     2941 attaatgaat gttgggcgtg gtatggtcat tacttcggaa gaagctattt tagagaacga
     3001 aaagaaacta acgcattgga cacctaatac ctatcgcttt ggtacttatg cggatgatta
     3061 tcgaacagtg gttaaaggtc attctgagaa aaacttaagt caaatcaaca catttgtagt
     3121 tgatatcgat tcaaaagaga accatcaagg cgaaattatt ttagcctgtt tagatcaagt
     3181 gggctacatg ccaacgctaa ttttagaatc agatcatggt tatcaagttt attttgtact
     3241 aaagacacca ggctatgtca caaagaaaag caatttcaag gtgatcgatg tagcaaaaaa
     3301 aatttcaaaa acaattcgtc tacagttggc tgaaaaattg tccggagtgg atctaggatg
     3361 taaccacttc ggaattgctc gattcccaca agaaaataat attgttttta aagaaatgaa
     3421 ctatcagtat tcttttgctg actggattca atggtcgatg aagcttgaat cagatcaaaa
     3481 aacaaaagaa gatcgagaac gaaaactaat tgtctttcct gagaaaaaag aaattcgtca
     3541 agtagatgaa ccgtggttcg atatgttact tcataaagca aacatcgtcg gcggagaagg
     3601 acgtcttggt cgaaataatg tgattttcac gctttcttta gcttattact cttcaggtta
     3661 tgcattggat acttgcgaat acaatatgtt cacattcaac gaacggttac aggaaccgct
     3721 atctgaagca gaattagtaa agattgtacg tagtgcttat tctggtaatt atcaagcagc
     3781 aagcagagaa ttcattattg aattgtgcca gcaatgggtt tcaccaacct taaaaaataa
     3841 tgatctgttc attcaacgac gtggctggtg gaaatttaaa aagccacgag atcaaagaaa
     3901 atatagtcat aaaaatgaat ggaaagaaga tctattggct tatctaagcg aaaaaagtct
     3961 tgctagtgag ccttatttga acctgtcaaa aaaagaattg gcttcacaac taacgatgcc
     4021 caagcgaaca cttgataaga tcttaaatga gttacgagag gagaataaag tcttctacaa
     4081 ggtaacacga ggaagaaatg gcggaatgat cattgcttca gtcaaaacgt tgttcgcgag
     4141 ggttattcaa ctaagtaaag aaaagagact agattattca acaaggatca cagaggtatt
     4201 tggactcaac gagagcttag tagaagagac atttagacaa ttatcaaccc ccttgaaccc
     4261 tcttaaagaa gtgaatcttt ttgaagtaga tacaggttga aaaataaaag gtgcaaattg
     4321 ccttatacaa tatatctatg atacttctgt aggtgttgct ccctttgcct agctacgtgt
     4381 aggtggtata cttataactg cgtatgcagt cttatgtatc atggaggaag ttgtatgtcg
     4441 ttaaaaaatt atgtctattc acatcctgtt aatgtgttta tcattggtcg tttgggatta
     4501 tctgtggaag tgttctgtga gctttatggt tttaagcagg ggactttgtc ttcttgggtt
     4561 accagggaaa aaacagttgc ttctttgccg attgagtttc ttcatgggtt gtccttagct
     4621 tctggggaga ctatggatca ggtatatgat tgtttgtgtg tgttagagca ggagtatatt
     4681 gagtataaaa tagctaacga gttaagaaaa aggaagaagt atatccagta gtagttttta
     4741 tttactctta attgtcaaat taagttagac aaatcatcaa tacttacgta actcaaaaat
     4801 acttccaaag gtgttcgata attcagtgat tttctaggaa tattatttcg tttcgatgcg
     4861 acagattgaa taaaagattc atctactgaa ttgaaatcca tagatttcaa taaaccatct
     4921 ctacgtaaca atccgttaga attctcgttt aggcctcttt gagacggcgt tcctggatca
     4981 gcgaaataaa tggcaatatc attggcatta ctgatctgtt tccaatttga aaattccttt
     5041 ccacaatcaa aagtgatgga tttgaataga tttttcggta cagattcaaa ccaatgattt
     5101 aatttgtttt caatatcaat cgcttgtcta ccacacggtt tcaatgtgat gataactttt
     5161 gataatcgct caactaaggt aattacagca cttttatgtt tcaaacctac gatagtgtca
     5221 ccttcaaggt gaccaaactc atttgagaat tggctataat ctttttcacg ttcatgaata
     5281 gagcgacgga aagcttgttt tccacgtctt tcttgatgtc catttggttt acgcttgcct
     5341 ttcatcggta agtcagaagg attaaatagc ccctttttga acatacgata aatggtacga
     5401 gcagaacaac gaataggaaa cgctgcacga ccaacaatca catcgggtgt ccatccttga
     5461 acaacctttc tttgaatata ttctgattgt tcctcaggta aaacaagcgg gcgtctacca
     5521 cagtttgatt tgtttttctt atactgttga tagtactcta aggctgattt tccttgcttg
     5581 aagaacaggt atactttatg gatcgtttgt cttgaacgat tcaagaaatg cgcagttttc
     5641 gcaacagatt gatttatttt gaaataagac tctatcatta caagttcatc cgttgtaaga
     5701 tgggtatagg tcatttgtac tcactcctta taattctttg gacggaacta ttttgagtgt
     5761 agcacaaatg acttttttag ttgtctagct taattttaca atcggcgtac ttaaaaaatt
     5821 ggttcattga gcaacctatc gctgctaaag tagcaaagga aaacgttaaa ggaaagaatt
     5881 tagggaagaa atcgaaatga aatcggacga ctggattgaa cacacctggc ttcctctgaa
     5941 aagaaatagc aaacgaaaga aattttgggt attgcaatta tggccattag gaaaactttg
     6001 tagttacggg gcacgactta ttcttgattt aaccgtttac tcgattggag tatttggaac
     6061 ggcttattac ttgataaatg atcccaatag gattgcagca tcagacattt ttacagtgct
     6121 aatgacgttg atttttgtca tgctattgtc tatttttact caattagtca tgcaatttac
     6181 caggcaaaaa ttaacagcag gaaaactttt caaaagccta ttcgatttaa ttgttgtaaa
     6241 tgcaattcta gcgactttaa taaaacaaat tgaacaatct ggaagaacaa caaaaggagt
     6301 gaaggatttt ttattgacgt ttattcactt tgtaaatgga cacatgtatt tgtttgaagt
     6361 gggtattatt ctcttttttg tcgggtatgt atccacgaaa cttattaaac gttcaattga
     6421 aaaagaaatt gaaattgacc tgattccgac acttcttggt tatgagtaca atgttgattt
     6481 tggaagatat gacgaattga tcaatacgga tatcgttcac acgagtatcc atgaatctac
     6541 tgttaagggc atcagatcta ttttaaaaaa tggccaggaa atgaaagcaa tagatcgaag
     6601 aaaagagatc tacaattttt atgaagtcga agcaagccta cgatatggca tcggaaaatt
     6661 gtggttagat ggaagtaata ttcgtcaaaa caaaccatac attttttgga acagtagtga
     6721 tttagaactt ccaatgaaag ataaataatt atggagaatc gtttgaaaat gattcctcta
     6781 tacaggattg tgcttatagt tggcatggag ttaaaaacct taaagaagga gcagttattc
     6841 aaagtgaaag aacgtcataa tcagttacta tgtagtaaaa cgggtacgat gaacattgaa
     6901 taccgaacag aatatttaga attaacgaat caaactgatt ttttgttgat tcgaaagcaa
     6961 caagagcaag aaaaaaatta atggctactt atagccaatt gattgatgaa tgggaacaat
     7021 tcaaggaatc taaaattcca gaacgtctct aaaagccttt atttgtaaag agcataagga
     7081 aacgtgggaa cactttttaa aaaaagtgtt ccaataggct gtgcctatta cgtttctcaa
     7141 aaataaatgt gggaacaaaa tatttaaaaa taggatagtg aaaggatagt gaaggatagt
     7201 aaaaggaggg tagtaggata gcaaaaagga taggctcctt ttcgctatcc tttttaaatt
     7261 ggaggaatta atatggaagt aagaataaga aatgtggatg agaaaatcgt aagagaaatt
     7321 gatcgaagat gtgacgaacg gttctgttgc aaagttttaa atctactatc aaataaggta
     7381 gaataataga aaaagat
//
LOCUS       DACDIG010000104         7257 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.104, whole genome shotgun sequence.
ACCESSION   DACDIG010000104 DACDIG010000000
VERSION     DACDIG010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.104"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(47..1473)
                     /locus_tag="IV247_002606"
     CDS             complement(join(47..900,900..1473))
                     /locus_tag="IV247_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
                     /protein_id="HAP8778631.1"
                     /translation="MAKYTFELKLKIVHDYLDGKGGSDYLAKKYSIKAPSQVKRWINA
                     YQEFGEEGLVRKRQKKKYSVQFKLDAIELYLTSELSYREVANTLNMNNPNLIASWMRQ
                     FREGGIDGLSKTKGCPPILSKKNEPKNKKKSTTKATSKERERIEELEKRVRSLQIENA
                     FLKELRKLRKQEAQQRRMKQSHESSQASEDRFKLVELLETLKFPKAIFMYWQKRLDRK
                     NPDQEIEAEMLKVREKHKDYGCLRMTNELRNRGFSINKKKVQRLIKKLGIKVTTYTRK
                     SRRYNSYRGQVGAVAKNRIHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLD
                     PFMDMFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYR
                     NKLKEYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYY
                     NNERIKQKLNWQSPVQFRKTTVTVV"
     gene            1716..2171
                     /gene="tnpA"
                     /locus_tag="IV247_002607"
     CDS             1716..2171
                     /gene="tnpA"
                     /locus_tag="IV247_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAP8778632.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            2436..2534
                     /locus_tag="IV247_002608"
                     /pseudo
     CDS             2436..2534
                     /locus_tag="IV247_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2757..2987)
                     /locus_tag="IV247_002609"
     CDS             complement(2757..2987)
                     /locus_tag="IV247_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAP8778633.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(3217..4035)
                     /locus_tag="IV247_002610"
     CDS             complement(3217..4035)
                     /locus_tag="IV247_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8778634.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            4196..4885
                     /locus_tag="IV247_002611"
     CDS             4196..4885
                     /locus_tag="IV247_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP8778635.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4899..6401
                     /locus_tag="IV247_002612"
     CDS             4899..6401
                     /locus_tag="IV247_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8778636.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            6414..6890
                     /locus_tag="IV247_002613"
     CDS             6414..6890
                     /locus_tag="IV247_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP8778637.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6987..7097)
                     /locus_tag="IV247_002614"
                     /pseudo
     CDS             complement(6987..7097)
                     /locus_tag="IV247_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2343 a         1149 c         1315 g         2450 t
ORIGIN      
        1 ggagtggaga aatccactcc ttttttcatg ctctaaaatg atatttttac acaactgtta
       61 cggttgtttt acgaaattgg acgggacttt gccagtttaa tttctgtttt atccgttcat
      121 tattgtagta atagatatag ctatcgatgg ctgattttaa ttcctcgaaa cagttataaa
      181 ctttcccgtg aaaaatctct tgttttaata agccaaaaaa attctccata agtgaattat
      241 ctaagcagtt ccctttccga gacatgcttt gaaagatttt gtactccttc aatttgtttc
      301 tataagcttt catttgatac gcccaacctt gatctgagtg aaacgtacga cgatagggac
      361 aatcgttagt ggtctggata gcttcctcca aaccttccat taccgctaat gcattgggct
      421 tttcagagat acgataggat aaaatctcac tattgaacat atccataaaa ggatctagat
      481 acaatttctt ttgacggata atgcctttgg catccacttc atggtattta aattcagttg
      541 tatccgtcgt gattttctga tggcagatac tggtgtagaa acgacgatgg atccgatttt
      601 tggctactgc tccgacttgt cctcgataag aattgtagcg ccgtgattta cgtgtgtatg
      661 tcgttacttt tattcctagc ttcttgatca ggcgctgaac tttcttttta ttgatagaga
      721 aaccgcggtt tcttagttca ttggtcatcc gtaaacagcc ataatccttg tgcttctccc
      781 gaactttaag catttccgct tcaatctctt gatctggatt tttacggtcc aatcgttttt
      841 gccagtacat aaagattgcc ttagggaact ttaaagtttc tagaagctca actaatttaa
      901 acggtcctcg gaggcttgcg atgattcgtg cgattgcttc attcgtcgtt gttgggcttc
      961 ctgttttcgc agcttcctca attcttttaa aaaggcattt tcaatttgaa gggaacggac
     1021 tcgtttctcc aactcctcga tgcgttcacg ttctttagac gttgctttag ttgtagattt
     1081 tttcttattt ttgggttcat ttttcttaga caagataggt ggacatcctt tcgtttttga
     1141 gagtccatcg atcccaccct cacggaattg acgcatccaa ctagcgatca aattagggtt
     1201 gttcatgtta agtgtgttgg caacttcacg ataggacagc tcacttgtta gatataactc
     1261 tatcgcatcc agcttaaatt gaacagaata ttttttcttt tgtcgctttc ttactagacc
     1321 ctcttcacca aattcttgat aggcattgat ccagcgcttc acttgtgatg gagccttaat
     1381 ggaatatttc tttgccagat aatcacttcc gcctttacca tccaagtaat catgaacgat
     1441 ctttaattta agttcaaaag tatacttagc cataaaaaat accgacctcc aaaagttaga
     1501 ttttttggtc taacttttgg gggtcggtac aaaacttaaa agttttagac ccttttattt
     1561 tcttaatgac ttcagtcagt tttgtgcgta aagcgtgcga aacaagaaaa ccgcgcgttt
     1621 ctacgcgtgg aagaagtatc tttagcgata aaaactcctt tcgatatact agtgagtggc
     1681 taccaaccgc tcatcagatc gaaaggagtt ttttcatgtc aaatgacgat aaaagtttag
     1741 catatactcg ttggaattgt aagtatcata ttatttttac accaaaatat caacgaaaag
     1801 taatctatgg gcaattacga aaagatcttg gaaaaatact taggaagttg tgcgaaatga
     1861 aagatgtgga aatcatagaa gcacatgcaa tgctagatca tattcacatg cttgttcgag
     1921 taccaccaaa aatatgtata tccagtttta tggggtatct gaaaggtcgt agtgcggtac
     1981 taatccacga gcagcacgca aatctcaaat ataaatacgg aaacaaatcc ttttggtcga
     2041 agagatacta tgtgagtaca gtcggattga atcaaaagac aaatgaaaaa tacatccgtg
     2101 aacaagaagc tgatgatcgc attcgatata ttataagtaa aagagagtac gtagaaccat
     2161 ttaaaaaata aagagaagta agtgagtgaa gatgagcagt tggcggtctt ttaaaaatcc
     2221 ccttttaagg ggcagcgagt gtcgacccct tctaggggga ttaaaaatac acccgtcctc
     2281 acgggtgtat gggttactga tagacttgat tttaactggt gtcagaaact tatgtccaga
     2341 aagggtgatg aaattttggc aatttacggc tattcaagaa ttagcacaag caaccaagat
     2401 tacaaaacac aaattcaaac attagaaaat gctggcgcag aaataatctt cctagaaaat
     2461 cactggatta tcgaacacct ttggaagtat ttttgagtta cgtaagtatt gatgatctgt
     2521 ctaacttaat ttgacaatta agagtatatt tacttcagat ggtaaggcaa caaaataaaa
     2581 tacaataggc agatgaacta ataaataaaa tctgtattaa atacagataa attaatataa
     2641 aaatcaaaag gatcataaac ttttaacaag tgatgaaatg ggtggaaatt gattcttata
     2701 gacagtttgt ctcataacat tgaagtattt tgcaatttta gcattgaaac acttttttaa
     2761 tcttcaacgt tgttcttata aacaagatga ttgggaatct tttgaattaa cactgtatag
     2821 cttagaacac cctttataga aaacattcgc ttagcaatct taataattcc ttgtgcccga
     2881 tgtcgtttag attcagtagg ttcctgctca tcgatcatgg aaaaaatttg aaggatcaga
     2941 tccttaataa atttatttaa gagaggatga tccattgctt catccatact cgataaacta
     3001 gtgataatga gctacacgtt tttctttcaa gttgatcaat agcttccacg ataaaaatat
     3061 ctttagataa aatcaagaac cgtaggaact acagggatat ttggtcaata agagccaccg
     3121 ctattagtaa aaaataagct atcaagtatg ctctcatccc aagaattgcc tacttcaatc
     3181 gttaaaaacc gttaggttta gctaagtggg ggtagttcac tcaatacttt tatcaataat
     3241 agagcgcaaa actttttctc ttaattcaac tgaatcaata ttattgtttt gttcataacc
     3301 atgaactaat agatcacaaa tataaagttg tgatattttt ccagctaaag atccaccatt
     3361 aagaaattct tcaatggctg tttgtaaaac taagtctgca gactttccta taggtgaatg
     3421 aatatagtta gttatagcaa taatttttgc gccatttttt ttagctattt tcaatgaatc
     3481 ataagtatct ttcgttttcc cagataaaga aaaaatgatt actaaatcat taactgttaa
     3541 taaagaagcg acttgggctt gaaaatgagg gtctagtaca gcttttgctt gaactcctac
     3601 gcgaagaaac atattttcta aatcaagact agtatttcca ctagatccca ctccaaatat
     3661 atatatgttt tttgattgag tgatcatttt aatggcttca ttcaattttt cttcgtctaa
     3721 aagcctaata gtggaataga gagcttctgt taaactatta gcaatttcat catagtactt
     3781 tacactagtc ggatgttctt ttttttgact aaagtcttcc tttgcaattt caatttttaa
     3841 atcagaaaaa ccagaaaaac caattttttg acaaaagcga ataatagtag catcgccaac
     3901 ttttgtgttt ttcttaatat cactcatcgt actataaata atagttttcc cagaatttaa
     3961 aataaaatca gcgacttttt tttcagactt cgttaaaaat ggataatttt ctttaattaa
     4021 atagatataa ctcatacaaa ctcctctaca atttcaactt ttctccatta tactccaaaa
     4081 taataaaaaa aactcctaaa tactccattt ttatctttac ttttatgaaa gcggatgcta
     4141 tattgttttt ggagttgtta tccaaaaata ataaaaaatt ggagtgttta ggataatgga
     4201 taaaaagagg tttttagatc ttatttcaaa aggtttgatt gtttcctgtc aagcattacc
     4261 tggtgaacca ttttacaaag aggaagaaag tttaatgcct tattttgctt tggctgctca
     4321 aagggctgga gcagttggta tacgtgcaaa ttctgttcga gatattattg aaattagaaa
     4381 gtgtgtatca ttaccaatga ttggaattat taaaagagat tatccaccag aatctccatt
     4441 cattactgcc actatgagag aagtagatga attagtagaa accggtgttg aggtaatcgc
     4501 tcttgattgc actcttagaa atcgacatga tggactatct ataaattctt ttattactaa
     4561 aataaaagaa aaatatcctc atcagttatt tatggcagat atttctacat ttgaagaagg
     4621 caaaaatgca tataaagcag gaatagattt tgtaggaact actttaagtg gatatactga
     4681 ggaaagtaaa aaacaagatg ggccagatat agatttgatt aaaaaattgg tagaagaagg
     4741 gatttgtgta attgctgagg gaaggattca tactccagaa caagcaaaag agataaaaaa
     4801 aataggagtt gcaggtattg ttgtgggtgg tgctattact cgaccacagg aaattgcatc
     4861 aagatttgtt aaagcattaa gttaggggga gaaataaaat gtttaaaaaa ttttcacaat
     4921 taggacgcgc tttcatgtta ccaattgcta tattaccagt agccggatta ctattgggac
     4981 tgggaggagc gttaacaaat gagagtgcta taaatgctta tccattttta gatcaaccgt
     5041 ggttgcatac aattttaagt attatgagtt atgctggaaa tgcagttttt gcaaacctag
     5101 cactaatttt tgcaattggt attgctgtag gacttgcaaa tggagataaa ggaacagctg
     5161 gtttagctgg tggagtagct tatctagtat atactgcaac tattagtggg tttcttgaac
     5221 ttttttcagc aaaagatgca acactagata caggtgtagt gggatcaatt gttgttggtg
     5281 gtactgttgc atttttacac aatcgttata gaaaaattga attaccacaa ttcttaggtt
     5341 tttttggagg atcaagattt attccgattg tttctagttt agctgcaatc attattggtt
     5401 ctttctttta tttaatttgg ccaccaattc aaaatggttt agtagttgct ggtgaacaaa
     5461 ttgcccaaat gggtagttta ggaacttttt tatatggttt tttattgcgt ttaacaggtg
     5521 ctgttggact acaccacacc atttaccctt tgttctggta tacatcttta ggtggtaccg
     5581 aaactgtagc tggatcaaca attgctggag cccaaaatat tttctttgcc caactagctg
     5641 atccaaacca cactggtctg tttacttatg gtactagatt ttttgcagga agatttgcaa
     5701 caatgatgtt tggattacca gcagcatgtt atgcaatgta tcgtgcaatt cctaagaaaa
     5761 atcgtaaaaa gaatggtggg ctttatttta gcggggccct tacatcattt ttaactggaa
     5821 ttactgaacc agttgaatat atgtttttat ttgttgctcc ttggctttat gtgattcatg
     5881 cattcttaga tggattatct ttttattttg ctgatatctt aaatattcgt gtagggaatt
     5941 ccttttcagg tggtttgatc gactttttat tattcggtgt acttcaaggg aatgacaaaa
     6001 ctaattggtt aaaattaatt ccttttggta ttgcatgggc atttatatat ttctttgttt
     6061 ttacattctt tatcaaaaaa tttaaggtag ctattcctgg aatggaagaa gaatcagaag
     6121 ttcattctga aaaagtaata aacaaaaata catcattaca tgaagagtct cttatcatta
     6181 ttgaagctct tggtggaaaa gaaaatattg agtctgttga agcttgtgca actcgtttgc
     6241 gtgttgctgt tacgaatggg gagattattc aaaaacaaac aataaaagac actggtgcaa
     6301 ctgctgtctt cgaagtgaag ggtggcatcc aagctgtttt tggagggaaa gctgacttat
     6361 taagtcaaga aatcaatcaa atattaggaa aagataatta ggaggttttt aatatgtttg
     6421 aaattttcaa aaagaaaaaa caagaaactt tttatgcacc ttgttccgga aaaatcattt
     6481 ccatcacaga agtaaatgat cctgtctttt cacaaaaatt actaggtgat ggttttgccg
     6541 tcatacctga tggttcaaaa atttatgctc cattggctgg tcgagtaaca aatatatttc
     6601 ctactaaaca tgctattaat ataaaaagca actcaggcat tgattatttg attcatattg
     6661 gaattgatac agttgaatta caaggagagc ctttttcgat ttgtgttcaa gaaaatgatg
     6721 aggttactcc tgaaacctcg ttggttgatg tggattttga caaaataata aaatctggta
     6781 aagatacttc tgttatagtt gtttttccgg agaaaaatca gttcaatgat ttaataatag
     6841 ataatggtcg aattgctcat gggaattcat gtggaaaaat cactttttaa aaaagtgtat
     6901 ataaaaagct ataaaataag ctaaatagga tatttgtcaa atgaagttgg tgaacagaaa
     6961 gaactgtttt ctttctgttc atttttttat tttattagta atgattcccc atgccaaatc
     7021 atgatccgta atttaaagtt ataaaagctt cgaaatccat tggcatttcg ttggaatacc
     7081 ttgatatgat tattcaaata taaaatatag agagaagaga gagagggaag atatttataa
     7141 agggcaaagt tatgtttact caacttagga aatagaaata gatcaattta tattctcttg
     7201 tcaatcgaat atgaacctta gcactatata ccaaatatag taaaatgctg aaagatg
//
LOCUS       DACDIG010000105         6917 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.105, whole genome shotgun sequence.
ACCESSION   DACDIG010000105 DACDIG010000000
VERSION     DACDIG010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.105"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(48..506)
                     /locus_tag="IV247_002615"
     CDS             complement(48..506)
                     /locus_tag="IV247_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP8778638.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(519..1844)
                     /locus_tag="IV247_002616"
     CDS             complement(519..1844)
                     /locus_tag="IV247_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAP8778639.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1861..3273)
                     /locus_tag="IV247_002617"
     CDS             complement(1861..3273)
                     /locus_tag="IV247_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8778640.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(3290..4054)
                     /locus_tag="IV247_002618"
     CDS             complement(3290..4054)
                     /locus_tag="IV247_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAP8778641.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4158..5033)
                     /locus_tag="IV247_002619"
     CDS             complement(4158..5033)
                     /locus_tag="IV247_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8778642.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5288..5372)
                     /locus_tag="IV247_002620"
     tRNA            complement(5288..5372)
                     /locus_tag="IV247_002620"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5336..5338),aa:Leu,seq:caa)
     gene            complement(5390..5461)
                     /locus_tag="IV247_002621"
     tRNA            complement(5390..5461)
                     /locus_tag="IV247_002621"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5427..5429),aa:Gln,seq:ttg)
     gene            complement(5471..5543)
                     /locus_tag="IV247_002622"
     tRNA            complement(5471..5543)
                     /locus_tag="IV247_002622"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5508..5510),aa:His,seq:gtg)
     gene            complement(5605..5689)
                     /locus_tag="IV247_002623"
     tRNA            complement(5605..5689)
                     /locus_tag="IV247_002623"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5653..5655),aa:Leu,seq:caa)
     gene            complement(5710..5780)
                     /locus_tag="IV247_002624"
     tRNA            complement(5710..5780)
                     /locus_tag="IV247_002624"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5746..5748),aa:Cys,seq:gca)
     gene            complement(5826..5897)
                     /locus_tag="IV247_002625"
     tRNA            complement(5826..5897)
                     /locus_tag="IV247_002625"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5863..5865),aa:Gln,seq:ttg)
     gene            complement(5909..5981)
                     /locus_tag="IV247_002626"
     tRNA            complement(5909..5981)
                     /locus_tag="IV247_002626"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5946..5948),aa:His,seq:gtg)
     gene            complement(5984..6057)
                     /locus_tag="IV247_002627"
     tRNA            complement(5984..6057)
                     /locus_tag="IV247_002627"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6023..6025),aa:Trp,seq:cca)
     gene            complement(6064..6144)
                     /locus_tag="IV247_002628"
     tRNA            complement(6064..6144)
                     /locus_tag="IV247_002628"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6108..6110),aa:Tyr,seq:gta)
     gene            complement(6146..6221)
                     /locus_tag="IV247_002629"
     tRNA            complement(6146..6221)
                     /locus_tag="IV247_002629"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6186..6188),aa:Phe,seq:gaa)
     gene            complement(6257..6329)
                     /locus_tag="IV247_002630"
     tRNA            complement(6257..6329)
                     /locus_tag="IV247_002630"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6294..6296),aa:Asp,seq:gtc)
     gene            complement(6339..6411)
                     /locus_tag="IV247_002631"
     tRNA            complement(6339..6411)
                     /locus_tag="IV247_002631"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6376..6378),aa:Val,seq:tac)
     gene            complement(6427..6498)
                     /locus_tag="IV247_002632"
     tRNA            complement(6427..6498)
                     /locus_tag="IV247_002632"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6463..6465),aa:Glu,seq:ttc)
     gene            complement(6513..6602)
                     /locus_tag="IV247_002633"
     tRNA            complement(6513..6602)
                     /locus_tag="IV247_002633"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6566..6568),aa:Ser,seq:gga)
     gene            complement(6642..6715)
                     /locus_tag="IV247_002634"
     tRNA            complement(6642..6715)
                     /locus_tag="IV247_002634"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6679..6681),aa:Asn,seq:gtt)
     gene            complement(6725..6840)
                     /gene="rrf"
                     /locus_tag="IV247_002635"
     rRNA            complement(6725..6840)
                     /gene="rrf"
                     /locus_tag="IV247_002635"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         2063 a         1540 c         1139 g         2175 t
ORIGIN      
        1 taaattcacc cataaatatc accctattta acttttttca tcgtacacta aaattcaaaa
       61 caactatctt taatctttac ttcttggttt tgttttaata aatttccttc aaaaatgcct
      121 ggagaaacta caatgatcac cgtatctttt tgtgcttgct ccaacttctt caaatctata
      181 atagctaaca gtgtcccttc ttttacctcc tggttacttt caacccgtat ctgaaacgga
      241 cttccttcga gttcaactgt atctaaaccc atatggatca gtatttttct gccagattca
      301 ctttcgatcg ttatggcatg tcgggtagga aatatattat cgattattcc tttaactggc
      361 gcatggatat atccatcatg attcgttata gcgtatccaa ctcctatcat ttttgaagaa
      421 aagacctcgt cttttactgc tgttaatgga ataatttgac cactagctac agcttttaga
      481 atgtttcttg ttttaaagaa atgcactttt tatcacctct actttaatgc tggccaatat
      541 tttttgttta ctttgatcat ttcatctagt attttttttg cgactctggc atttggtacg
      601 gtttgattca atgtaaatgc ttgtaaagct ttttgataag aaccttcaaa gtaagcatct
      661 actagtaact tctctactgc aacttgtgcc tccatcaatc ctttatgaaa atcactgatt
      721 ggttttctta gtgtaattgg ctccacacca gttgcaccaa catatgccgg tatttcaaca
      781 actgcatctg accgtagatt cggaatcgcc ccttgattag gaacgattag cataaagcga
      841 cgacgatcat cattcaacaa ggaaatagct atatcgacga tatattgtcc atgctcccca
      901 aaattaaagt tcaatacttc ttccacaata ccgcggcgta ttttatcagc catttctttg
      961 gtatttttct ctcgtccatc catgaccata ttagcacgtg tatactcttt gtcagtatgc
     1021 tcaacgacca tatcaggata taagtaatac tctaagtaat tattcgggat attacttgga
     1081 aaatttttcg tgatgactga catcatttga aaggtttttt gccaagtttt atctcctata
     1141 ttaaaatcgg cgacttgcat ttcttttgtt attagttttt caataatctc tggcataatt
     1201 tctcgttgca attctttatc ataaatcgat gtataccagc caaagtggtt taaaccataa
     1261 tacgtaacga tccaattctt acgatcataa ccgtaattca ctgcaatcgt ttcttcaatt
     1321 gaaatagtca tatcacaggc attaatcatt tttacattcg gatatttacg acgaacagct
     1381 tcagaaacga tcgactctgg atttgtataa ttcaaaatcc atgctttggg ggcatagtct
     1441 tgtatatgat cgacaatttc caataaacct ttgattgagc gcatcccata agcaaatcct
     1501 ccgagtccac acgtttcttg tcctactaat ccatgtttaa gtggaatctt ttcgtccttt
     1561 tctcgcatct ccaatcctcc aacacgaatt tgtgagaaaa caaaatcgca tcctgtaaaa
     1621 gcaagctttg gatcatccgt tgcacgaatg cgaatatttg aaaaaccttt tcttttcagc
     1681 ataaatttaa caatcaagta catatccata tttctttctt catcaatatc atataaacgt
     1741 atttcagata atggcaaccg tttttcattg ttcaaaatgg cttgaacgat tcctggtgta
     1801 tacgtactcc cgcctccagc tatagtgata atctgttttt tcatcatcat cttttccttt
     1861 ctattcttct aataatttat tgaattctgc cataacataa gtcacttcta agccgatgat
     1921 aatttgataa tttttttcag ttggtttaat gatcccgctt atccctaatt gcttgattcg
     1981 ctgttcctcg accatggtgg gatccactac ctctacccgt aggcgtgtat tacaactata
     2041 gacatccagg atattggctt ttcctccaaa cgcttgtagc atttttgttg ccatatattc
     2101 ataattttta cttgctaacc ctaactctgt ttcttctaag gttacttcat tgccaaactc
     2161 tgccttagct tcacgtccta atgtctgaat gtttctctta ttgatcaaat aataaaaagt
     2221 aacgtaatac aaggcaaaaa agaaaattcc gattggaaga atcaaaatag cattggtagc
     2281 gattcgaaaa ttgatgagat aatcaacaat acatgctccg aatgtaaaac caattcgtat
     2341 attgaataag taacagacaa tccctgctaa accggcaaaa agtgcatgga tcacgtacaa
     2401 cataggagaa agaaacatga aactaaattc aacaggctca gtgatatttg caataaatga
     2461 agttcctgca ccgcttccca tgattccttt gaccatctct ttattttctt tataagctgc
     2521 tttatagata gctagacata tagctggtat accaaacatc attactacaa aaaaaactga
     2581 taaaaacagt ccagcctgag gatctccgtt gataaagcgt gtcatctctc ctctgaaaac
     2641 ttccttgcta ggagatgtat agcttcctaa atcgtaataa atatacgtat tcaacacatg
     2701 gtgcagtcca aaggggatca gcaaacgatt caaaaacata aatatcccca cacctaatgc
     2761 tcccatatca acgagcagtt ttgcaaaatt atcaatccca atttggatga gcggccagat
     2821 aaagcccaaa aaaacagctg cgattgtttg tagaatcatc accatagtca atgggaattt
     2881 ttcgcccgca aaataagaaa aaactactgg taatttttgt tctttaaagc gattatacgt
     2941 ccatgccgct actaaaccgg aaataattcc accaaaaatc cccatattca cttctgggtc
     3001 cataatttct aagccttttt tcaaggtagc aatcgctaaa aaaccagtaa gtgcaggtat
     3061 tcctttgtcc ttatttttga caaaaccgat tgcaactcct atagcaaata agatatccaa
     3121 gtttccaaaa acggcatttc cagctgcacc aattactggg atgttcagca tatcgtcagc
     3181 tgataaacgc atgatcaatc ccgctaatgg catcgctgca actggaagta gcatcgctct
     3241 tccaagcttc tgcacatttg atttaacatt catttttatt cctcctctat taaatatcct
     3301 catatgttac taactcaatc aaaggatcat ttttcacttt ttgagcaaat gctgaatgaa
     3361 ttaaaaattc gacttggtgg gtacgaggat acacaaaact tgaaccattt aacaattcct
     3421 gatccaaata cgctgcgtga cacatgactt cgatcgtttt atcttctctc aatatcatga
     3481 gtaaacttgc caaatattct tccacactcg tattgatttg tcgttttttt tcatcagttt
     3541 ctcctacgac atcgaaacaa ggctcaaaat aatcgacatg acgaacttca tcttttcgtt
     3601 caaagttgct ccgaaccggc aaatcatatt tttttgctaa tgtaacaact acctcctgat
     3661 tttcatccaa agtatgagta tgatgatgac tgtctaaatg agttggtatg atccccgcat
     3721 gataaatttt ttgaatctga gcattccatt cctgatacag ttgatctttg tcaatgttaa
     3781 atggatgctg ataataagat aaggatctga actctccgct ttcgataaga gtatcgacag
     3841 ttttgaggag tggtttgcca cacgtcaatg ttaaatggac tcctacacct agcctaggca
     3901 tttcctttct tagtttcaca gcatgctcga aacccggcat atttgccatc aatgtagttg
     3961 atgtcaaaat tcctcgctga taagcatcca taatcccata attcacacca tggctatagc
     4021 caaaatcatc cgaattaaaa atcacttttc ccatttcatt ctccttcttt tttctactaa
     4081 aataggcgca accaaataat catagtccat ttactatgtg tcatttggtt gcgcctatag
     4141 atagttacgc ccttatttta atagattctt gctagatgaa ttgccaaata gatgatttca
     4201 ttttcttgaa tggcaagttg aaattgctgt tcgatgatat ctttaatctt tagtgctgta
     4261 caatacgctt tgctatgttt aaatgtttca gtaaaaaaac caatattttc tactggcgtt
     4321 tcttcctgat aaatcaagcg tttaaccagg aatttgatat gaaccattaa gcgatgataa
     4381 gtcagtgatt ctttatcata aacgtttgaa taggattttt ctagatggga caaaatcgtg
     4441 gtcgtcattt ctactatttt taagctcttt aaactattag gctctcctag ctcagcatta
     4501 atgatatgca tagtgatgaa tcctacttca tttgacccta actcggtttg atatcgatga
     4561 ttgattcgct cgaccacttc tactgctata tcatattcat ttggataaag gaatctcatt
     4621 tctggcaaca taattgctgt gacatctcta tcttctcttt ttcgttgaat agcaaaataa
     4681 atatgatctg tcaatgtaat atagatattt gaatctaatg gagaggagat cctagctgtt
     4741 atcagttcaa tcacttcttc cgcaaaatcc ataaattcga ttggaatctg cgagactatc
     4801 tcactgaatc gtttgatctt ttctttagaa tctaatacaa ataatttgtc agcatcctca
     4861 acatcaaatg gcttactcac tttagcagaa aagccaatcc ctttccccat cgcaattttt
     4921 tctattccat cgtcatcgat caatacagca ttatgattca gctttttcat cacatacacc
     4981 gttaattcac ctcctctata tgataaaaca aaacaacttg cagactcctt catacatatt
     5041 tttgtctcca aacaattttt aaagacaaaa aacgacctaa gaaagaaccc tttcttaggt
     5101 cgtgcctaat tgaatagtag caccctacat aatttacaaa aatagtttaa cacataataa
     5161 aagcgttatc aatatctttt ttactcgttt tatgataata cttgtatcga attgatttgg
     5221 aggtaattac ataaaaacaa aaaaaatata gcaactctct ataagaaagt tgctatattg
     5281 ataattatac cggcggccgg ggtcgaaccg gcacgtcctc aacggacact ggattttgag
     5341 tccagcgcgt ctgccaattc cgccacgccg gcaaatttaa aaataataac tggggtagct
     5401 ggattcgaac caacgcatga gggagtcaaa gtcccttgcc ttaccgcttg gctatacccc
     5461 aagaataaag ggcgaatgat gggaatcgaa cccacgaatg ccggagccac aatccggtgc
     5521 gttaaccact tcgccacatt cgccatcata aaagatgtta tttaatttct caataaaaaa
     5581 ctaagatgaa gacatcttag tcaataccgg cggccggggt cgaaccggca cgccctcaac
     5641 gggcactgga ttttgagtcc agcgcgtctg ccaattccgc cacgccggca agttgattta
     5701 ttagtactaa ggcggtaacc ggatttgaac cggtgatgaa ggttttgcag acctctgcct
     5761 taccacttgg ctataccgcc attatactat tatcaatcta taaaataaag aataaattat
     5821 agaaactggg gtagctggat tcgaaccaac gcatgaggga gtcaaagtcc cttgccttac
     5881 cgcttggcta taccccaaga ataaaaaggg cgaatgatgg gaatcgaacc cacgaatgcc
     5941 ggagccacaa tccggtgcgt taaccacttc gccacattcg ccatggcagg ggcagcagga
     6001 attgaaccca cactaacggt tttggagacc gctgttctac ctttaaacta tgcccctata
     6061 aaatggagga gagtggattc gaaccactga accctaagga acggatttac agtccgtcgc
     6121 gtttagccac ttcgctactc ctccatggtg gcgcgggaca gaatcgaact gccgacacac
     6181 ggagcttcaa tccgttgctc taccaactga gctaccgcgc catattttaa aattattaat
     6241 tgaataacaa gaacgacggt tccgacggga atcgaacccg cgatctcctg cgtgacaggc
     6301 aggcatgtta acccctacac cacggaacca gttcattatg gaggttaacg ggatcgaacc
     6361 gctgaccctc tgcttgtaag gcagatgctc tcccagctga gctaaacctc caacattcaa
     6421 ttaaaatgac ccgtacggga ttcgaacccg tgttaccgcc gtgaaagggc ggtgtcttaa
     6481 ccgcttgacc aacgggccag gtaaatttgc tacggagagt aagggattcg aacccttgag
     6541 acagtttggc accatctaca tgatttccaa tcatgctcct tcggcctctc ggacaactct
     6601 ccagagtgaa agaagctcaa tactctttct caaaatgaga actccggcag taggactcga
     6661 acctacgaca tcatgattaa cagtcatgcg ctactaccaa ctgagctatg ccggaataac
     6721 atcagcgtgg cgacgtccta ctctcacaag gggcaacccc tcactacaat cggcgctaag
     6781 aagcttaact tctgtgttcg gcatggttac aggtgtatcc ttctcgctat cgccaccaca
     6841 ctgtggtgtt atcttttatt gagtaaattt tgttcactca aaactggatt tgaagtcaca
     6901 taagtttttt tccgagt
//
LOCUS       DACDIG010000106         6810 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.106, whole genome shotgun sequence.
ACCESSION   DACDIG010000106 DACDIG010000000
VERSION     DACDIG010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.106"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            80..3307
                     /gene="fss3"
                     /locus_tag="IV247_002636"
     CDS             80..3307
                     /gene="fss3"
                     /locus_tag="IV247_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAP8778643.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYCQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            3420..3734
                     /locus_tag="IV247_002637"
     CDS             3420..3734
                     /locus_tag="IV247_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8778644.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            3747..4121
                     /locus_tag="IV247_002638"
     CDS             3747..4121
                     /locus_tag="IV247_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8778645.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            4131..4466
                     /locus_tag="IV247_002639"
     CDS             4131..4466
                     /locus_tag="IV247_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP8778646.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            4549..5889
                     /locus_tag="IV247_002640"
     CDS             4549..5889
                     /locus_tag="IV247_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP8778647.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            5967..6641
                     /locus_tag="IV247_002641"
     CDS             5967..6641
                     /locus_tag="IV247_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8778648.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
BASE COUNT         2309 a         1130 c         1504 g         1867 t
ORIGIN      
        1 ttttaaaaag actttataaa caaagatgtt caagcgagca tcttattttt ttgtgcttaa
       61 tttgaaagga gaacatttca tgtataaaaa acaaaaaaag aaattatgtg tattaactat
      121 tttgagtttg gctgttcaag caattgttgg agcaattgct cctacgatta cttttgctga
      181 tgaaattacc catccacaaa cggtaaccgt agaactagat ttagcacatc aatatgctgt
      241 tgaaggaact tttagtgatg gtcgtccaat gtcagaagtc actgttccgc attatgcagt
      301 gtacaacggc tttaaacaag atattttctg tattgaacca ggagtcccga tttacaatga
      361 gttcactcct ggctatgaga aaaacccgtt accagatatg tccgataaag ccaaattagt
      421 ttccgtttta tggaaaaaag taggtacaga tgcagatact catattgtgg cacagaagat
      481 gatctggcaa gaagttaatg ggtataccct tcactcaata aaacgatcag atggtagtgc
      541 cgtaaatatc gcagccattg aggcaaaaat aaatcaagcc attgacgatt atcagaagaa
      601 accaaccttc cataatagta cggctaaaac agtattaggg caatcgacta ctgtgacgga
      661 tacgaataat ctgaatttgt cagagttcga tgaggttgtg gaaaatgccg caaacattga
      721 ttatcgtgtg aatggtaatc agttgataat caccccaaat gccaattcaa aagaaagtgg
      781 tgtgctgacg cttaagaaat ctgctggtac cggaacacct gtcgcctata aaaaggctgg
      841 ccaacagact ttaatggccg gggcgattga taaacctaat acctacacaa tcaaaattga
      901 tgtggaaacg gagggttctt taaagatcaa aaaagtcgat aaagaatcgg gtgatgttgt
      961 accaggaacg gttttccatt tagactttgg aaaaacttta cctgcaaaag acgtgactac
     1021 tgacaaagaa ggcattgcta cattggatga gattcctcat ggtacaaaag taaccattac
     1081 cgaaaaatca gtaccggcgc cttatacgat tgataccacc cccatgacta ccaccattaa
     1141 agcaggcgaa accatttacg tgacttcaaa aaacgcacgg gaaaaagggc aaatcattct
     1201 ggataaaagc ggagttgaaa cagggagtga tctttggaat gacaattatt ccttagctgg
     1261 taatacgttt gccattcgca aagacagtcc aaccggtgaa atcgttcaag aaatgaccac
     1321 ggatgaaaac ggtcatgcgg aaacaccaaa agagattgct aatgcgttgg aattgggaac
     1381 ctattatgtg accgaaacta aagctagtca tgggttcgtg aatacgttca aacctgtaaa
     1441 agtcgaattg aagtatgcca atcaaaccgt agcccctgtt accagtaacg taaaagggca
     1501 aaatcaagaa gtcactggcg aaaccacttt gacgaaagaa gacaaagata ctggggataa
     1561 agcacaaggt aaggcagtct ttgagggtgc tgaatacacc ttgtttactg ctaaggatgg
     1621 aaaagctgtt aaatggagtg aggcttttaa accagagtta gcgaagggaa cgaaaggttc
     1681 tgatgaaacg gtcactttga ctttagacga aaagaaccaa gttgccgtta aacatttagc
     1741 tatcaacgag tattattgcc aagaaacaaa agcgcccgaa ggatatagcc ttgatgaaac
     1801 aaagtatcct gtatccatca aaaaggtgga tgatagcgaa aaaaatgccg tgattacccg
     1861 aaatgttacg gcaaaagaac aagtgattcg ctttggcttt gatttcttta aatttgctgg
     1921 atcagctgcg ggcactaccg aaactggctt caatgatctg acctttaaag tgtcgccatt
     1981 ggaagggacc aatgaaatca caggtgccga agatgaagcg accacagctt ataacgagca
     2041 attagggttt gatggttatg gcaagtttga aaatcttcct tatggggatt atttacttga
     2101 agaagtagaa gccccagaag gttttcaaaa gattacgcca ttagaaatcc gttctacttt
     2161 taaggaaaac aaagaagact ttgtgaagag tgagtatgtc tttaccatta ccgaacaaga
     2221 ccaaaaacaa ccaattaaaa cggtgaccgt tccttacgag aagctgacga acaaagcatt
     2281 ttctgttagt ttgaaccgtt taatgctcta tgatttgcct gaggaagaag atagtttaac
     2341 ttctcttgcg acttggaaag acggaaacaa agaattaact tctctggatt ctaccgagct
     2401 agttgataaa ttgagttaca atctacatga aatcaaagaa gactggtatg tggtagcgca
     2461 agccattgat gtggatgcga caaaagctgc ccaagaaaaa gatgaaaaag ccaaaccagt
     2521 ggtgattgca gaaacaagcg caacgttggc gaacaaagag aaaacaggaa cttggaaaat
     2581 tcagcataaa ttaaccgccg aacaagtttt aaataaaact attgtcttat tcaactatgt
     2641 gtatgaaaat aaggaagctt ttgaagcagg cgataagcca gtagcgaagg atgttagctt
     2701 gaataaccaa gcccaaactg tcagttgtac agtggaacac catgtttcta tccaaacaaa
     2761 ggcccaccta gaaaacggtt ctcagacctt tactcatggt gatgtggtgg atatgtttga
     2821 tgatgtgtcg attactcatg atgtactgga tggctcaaaa gaagctttcg aaacaattct
     2881 gtatgcttta ttaccggatg gtacaaacaa agaaatttgg aaatctggca agattgatta
     2941 tgaagtaaat gataaagaat tcaccaaaac tgtgcttgcg aaaaaagtag ataccggaaa
     3001 atatccagaa ggaaccaagt ttacttttgc ggaaatcaat tacgataaag atgggaatat
     3061 caatggaaaa cacaatgaag atttgaaaga aaaatctcaa accttaacac caaaagaagt
     3121 tccaaccata ctaagtacgc cgaaacaacc ggaaacacca actgttccaa gtggctctca
     3181 agaatctagt cccacagtga agaccttccc tcaaaccggg gagaaaaatt ccaatgtttt
     3241 actgttcatt ggttttactt tgatttttgc gacggcgggc tattatttct ggaatcgccg
     3301 gaactaaggt gatgagatga tacaagtaaa tcctaagaac caacgtgcgc gaccgcctcc
     3361 taaaaaagta gtcgcgctat ttttgtatcc aaaactaaca atgaaaagag gaaaagaaga
     3421 tggaattgaa atttgtcgtg ccagatatgg ctgaaacctt tggaaagtta agttatgccg
     3481 gtgaaggaga aattataaca gaaggatatg gacggaatac tacggtgatt ggtcgcagtt
     3541 atcatttgta ttccagtaag caacgggccg atgatattga ggtggtagta gctgcggaag
     3601 ccggtgaaaa ggattttgat caagaccaac cgctaaaagc cgtgaatccc catttggttg
     3661 ccaaaggcta tgagattgag aatcgtgggt ttactgatta cgtgttgtat gttgatgatt
     3721 tagtgaaagc ataggaggaa aagaagatga gattaacaga aggtattgtc gtagattcag
     3781 gtttaacatt tgggaagttg cgtttttccg cattacgccg agaagtacgg aagcaaaatg
     3841 aggatggaac aattggtaat gaggtaaaag aacgaaccta taatttaaaa tcctctgcac
     3901 aagggcgaat gattcaagtt agtatcccag ctaatgtacc cttacgcgag tttgcttatg
     3961 atgcagaggt agagttggtc aatccaatcg tggataccgt tgccaattat gtttttcgag
     4021 aaggaactac cgttaattgg ttcattaagg cggatgattt agtcttgaag cggcagccga
     4081 atcaaggaaa tcctacgaac caaaatgaaa gaaagaaata ggtgaagatt atggaaatta
     4141 atgttgaaca tattttagat tgtctcgatc aatatggaaa aggcgagctg actgaggaac
     4201 aactcgttaa agcattaacc tctgatgaaa aaatgtttct gattatacat caaggtttac
     4261 ttggagaaaa tagcgacagt gaagaagact ctgatgtact agactggtta ggagaagaag
     4321 agtctttctt tatagttgtc gaagtaaatg aaaacttatg tcggcaggca gaatccattt
     4381 tggaagaaat tggtgtggag atgccggatg ctattgaagg ctttttaaat caattggttg
     4441 aaacgaaaca actcccctcc tcgtagcagt aaatgactag cataactttt tgaaattgcc
     4501 taatgaaatc ctattgggct ttttcgattg gaggtggaaa ccatcaaaat gtacaaagga
     4561 catcgcatac gcgcaggaga tcagcacttg gtttatcact ttgttcttgg ctggctactc
     4621 gctctgttca tcggttggat gagcgtcttt tattttcaag agtttagaca atttgatatt
     4681 tctaagctat cactttctac tattgaaatc gtttggtcta taaaagattt agtatgtctg
     4741 cttggaagtc tcgctttttc aggagctatg atattgcttt atattcactt ttttcttgat
     4801 cattggcgaa gcttgtggca tcgacaaaag ctagctcgga tgattctaga gaaccattgg
     4861 tatgaagtga aacaaactca aagtgaaggc tttttcaaag atttgaatag tagccgaacc
     4921 agagagacta tcagttactt ccccaaaatc tgttatcgaa tgaaagatgg attactgtct
     4981 attcgcgttc aaatttcact tgggaaatac caagatcagc ttttaaaatt ggaaaagaag
     5041 ttagaaagcg gactgtattg tgagttagta gaaaaggaac tcaaagactc ctatgtggag
     5101 tatactttac tgtatgatat gatcgccaat cgaattggga tagacgaagt agtggcagaa
     5161 aatgggactc tgcgattgat gaaaaatcaa gtatgggctt atgattcttt accccatatg
     5221 ttaatcgctg gtggcacggg tggagggaag acctatttcc tcctcaccat tatcgaagca
     5281 ctattgaagt cagatgcaga attgtttatc ctcgatccca aaaatgcaga tttagctgat
     5341 ttaggtacgg tgatgcctca tgtttattca caaaaggaag aaatttctgc ttgtgtggaa
     5401 gatttctatg aacgtatgat ggctcgtagc aaggcaatga aagaaatgtc taactacaaa
     5461 acaggagaga attatgccta tcttggactt ccacccaact ttttaatctt tgatgaatac
     5521 gtggcttata tggagatgtt aacgacgaaa gaaagtgcgg tgattttgaa taagctcaaa
     5581 caaatcgtga tgttgggtcg tcaatctggt ttctttctga ttttggcttg ccaaaggccg
     5641 gatgcgaaat atttaggaga cggaatccgc gatcaattca actttcgcgt ggctttgggt
     5701 cgtatgagtg aactcggata ttccatgatg tttggggagg tcgaaaaaaa cttctttatg
     5761 aagcacatca aaggtcgagg ttatgtggat acaggtggca gtgtgattag tgagttttat
     5821 acgccacttg taccaaaagg gtatgatttc ttagattcaa ttagtaaatt agaaaagatg
     5881 aatgattgag tgatcttcaa tactgacttt atacttcaag taacgtataa tacttttata
     5941 tagtaggaag aaaggggata attaatatgg aaagtaatga ttcttattta gagtatgcaa
     6001 aagcaaataa ggataagttt atttcttata ttatcaaagg gaaatccccc gaagttggac
     6061 gagacgcagt ttttatggca gggagtcctg gggctggaaa atcagaggtt gccattggat
     6121 tagctgagaa ttatgataac catgtgatta ttgatgcgga ttactttcgc acccagtttc
     6181 ctgattataa tggaaaaaac tctagtctct ttcaaaaagc atcttcttgg ttggtagagc
     6241 aggcatttag atatgtctta gatcatggct attcttttat tctggatgca acttttgcaa
     6301 tattaagtgc tgaaaaaaat atctcgcggg cgttgaaaaa acattttcga gtgacgattt
     6361 tctatgttta tcaagaccct aaaatcgctt ggaaatttac acaagaacgt gaaattgcag
     6421 agggacgtca tgtgccgaaa aaggcattta ttaacgcttt tttcaaatca agagagaaca
     6481 ttgcaaaagt aaagagtaaa catccagatg tattgcttca tatcataata aaagattata
     6541 cgaataatat ttccgaagtt cattatgcta cggataatct agaattattt ttaccactag
     6601 ggtatagtag gaaagatttg gaggttcaat tacatgactg agaaagaaca agtaacgaaa
     6661 attgtaaaga aatacaataa gagcattgcg gatttatcgg aaaatgctac tgcaaaagag
     6721 ttcaaaacgg tgattaaata tgtagccgat caggccaatg aaaaacaacg gaaattagtt
     6781 ggattaaata aaaaatagta ggacaaatta
//
LOCUS       DACDIG010000107         6784 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.107, whole genome shotgun sequence.
ACCESSION   DACDIG010000107 DACDIG010000000
VERSION     DACDIG010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.107"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1517
                     /locus_tag="IV247_002642"
     CDS             <1..1517
                     /locus_tag="IV247_002642"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP8778649.1"
                     /translation="YLLGLIFYKYLSDRLLEQVVLLADESLEEYDTVSKQTMLYRELL
                     SDEESKEDLIATIVDILGYAIAPEYLFNVLADQAKQATFQLNDLNKAFVQLASTYNQF
                     NGLFDDVDLQSKKLGTDEQQRNVTITEVIKKLNDVDVLEHDGDVIGDAYEFLISQFAS
                     EAGKKAGEFYTPHMVSDMMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNYLTHPDN
                     VKYHGQELNTTTYNLAKMNLILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMNPP
                     YSANWSADTTFLDDSRFNRYGKLAPKSKADFAFLLHGFYHLKETGTMAIVLPHGVLFR
                     GAAEGVIRQKLLEDGSIYAVIGMPANLFFGTSIPTTVIVLKKNRQTRDVLFIDASREF
                     VKGKNQNKLSEENIQKILETYAERKDVEKYAHLATFDEIKENDYNLNIPRYVDTFEEE
                     EPIDMVHVGNDIKKIRQEQQVLEKKLLEAISSLQTTPENEAWLQGALEVFKHEQ"
     gene            1507..2724
                     /locus_tag="IV247_002643"
     CDS             1507..2724
                     /locus_tag="IV247_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP8778650.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            2737..5886
                     /locus_tag="IV247_002644"
     CDS             2737..5886
                     /locus_tag="IV247_002644"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HAP8778651.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
BASE COUNT         2365 a         1109 c         1380 g         1930 t
ORIGIN      
        1 attatttatt gggactgatt ttctataaat atttatccga tcgattgttg gaacaagtgg
       61 ttcttttagc agatgaatcc ttagaggaat atgacacagt aagcaaacaa acaatgttgt
      121 atcgtgaatt actttcagat gaagaatcaa aagaagatct gattgccaca atcgttgata
      181 ttttgggata tgcaattgca ccagagtatc ttttcaacgt cttagcggat caagcaaaac
      241 aagcaacgtt ccaattgaac gatctaaaca aagcctttgt gcaattggca tctacctaca
      301 atcaatttaa tgggttattt gatgatgtgg atctccaatc aaaaaaacta ggaacagatg
      361 agcaacaacg aaatgtgacg attaccgaag tcatcaagaa attgaatgat gtagatgttt
      421 tagaacacga tggggacgtg atcggggatg cgtatgagtt tctgatcagt caatttgctt
      481 cagaagcagg gaagaaagcc ggagaatttt acacacctca tatggtttct gatatgatgg
      541 cgcaaatcgt aacgctcgat caaaaggaac gacgattctt tagtgttttt gatccaacga
      601 tgggttcagg ttcattgatg ttaaacgtac gtaactactt aacacaccca gataacgtta
      661 aatatcatgg gcaagaacta aatacaacga catacaatct agcaaaaatg aacttgattt
      721 tacatggtgt cgacgccgaa gaaatgaatc tccgcaatgg ggatacgttg aataaggact
      781 ggccaacaga tgaaccttac acatttgatg cagtagtaat gaacccacct tattcggcta
      841 attggtcagc agatacgact ttcttagatg attctcggtt taatcgttat gggaaattag
      901 cgccaaaatc caaagcagat tttgcgtttc ttcttcatgg gttctatcat ttgaaggaaa
      961 caggtaccat ggccatcgtc ttaccacacg gagttttatt ccgtggcgct gcagaagggg
     1021 tcatccgtca aaaattacta gaagatggca gtatttatgc tgtgatcggt atgcctgcga
     1081 acttgttctt tgggacatcg attccaacga cggtgattgt gttgaagaag aatcgtcaaa
     1141 ctcgagatgt attattcatt gatgctagtc gtgaatttgt taaagggaaa aatcaaaata
     1201 aactatcgga agaaaacatt caaaaaatcc tcgaaaccta cgctgaaaga aaagatgtcg
     1261 agaaatacgc tcatttggca acctttgatg aaatcaaaga aaatgactat aacttaaata
     1321 tcccacgcta tgtagatact ttcgaagaag aagaaccaat tgatatggtt catgtcggaa
     1381 atgacatcaa gaagatacga caagaacagc aagtgctaga aaaaaaactt ttagaagcta
     1441 tctcttccct gcaaacaaca cctgaaaatg aagcgtggtt acaaggtgcg ctggaggtgt
     1501 tcaaacatga gcaatgatac acaaccagaa atccgtttcc caggatttac agatgattgg
     1561 gaacagagga agttggggga cttcgttgta gattatgttg aaaagacaag tgtacaaaat
     1621 caatttccta tgctaacttc atctcaacaa aaagggatag ttttgcaaga agactatttt
     1681 gcaaatcggc aagtaactac agaaaataat attggttact ttgtactacc tagaggatat
     1741 ttcacgttcc gtagtcgaag tgataatgat gtgtttgtgt ttaaccgtaa tgacattatt
     1801 gatagaggga ttataagcta tttttatcct gtatttactt taaaatctgc tgattccgac
     1861 ttcttcttaa gacgtattaa caatggaatc caacgacaat tatctattca agcagaggga
     1921 acaggtcaac atgttctgtc tctaaaaaag tttaaaaata tagtagcaat gtttcctagt
     1981 gaggaagaac aacaaaaaat cggcaccttc ttcaaacaac ttgacgacac gatcgctctt
     2041 catcagcgta agttagattt gctgaaggaa acgaaaaaag gattcttaca aaaaatgttc
     2101 cctgaaaacg gagcaaaagt gccagaaatc cggtttcctg gttttacgga agattgggaa
     2161 gagcgtaagt tgggagaatt aggtaaaact caatcgggaa ttggatttcc tgatgccgag
     2221 caaggtggtt ctgaaggtac tccatttttt aaagtttccg acatgaataa cattggtaat
     2281 gagtatgaaa tgagaaatgc aaatcattat gtatctaatg agcaaattga gcgtaaaaaa
     2341 tggaagccaa taaaagatgt gccagcgatt atatttgcta aagttggcgc agcaataatg
     2401 cttaatagaa aacgtttggt cacaagccct ttcttaatag ataacaatac catggcttat
     2461 ttatttaata atacttggga tatctatttt ggtaaaatat tgtttgaaac tataaatttg
     2521 ccaagatact cccaagtagg ggcattgcct tcttataaca gttctgatat tgagaatatc
     2581 agtgttaagg ttcctgtaaa ggatgaacaa caaaaaatcg gtactttctt caaacagcta
     2641 gatgatacga tcgctcttca tcaacgtaag ttagatttac taaaagaaac gaaaaaaagc
     2701 ttcttacaaa agatgtttgt ctaggaggga agaagaatga aaattagtca ccgtgaggaa
     2761 gcagaagtcg aagaacagtt gattcgtgta cttggagaag ggcataatca gtggacgtat
     2821 cgtccggatc taaaatcaga agaagacctt tgggtcaatt tacgccaaaa aattatctca
     2881 aataatcaag cagaactgaa tgattttcct ttgaccgata aagaatttga aacgatcaaa
     2941 acggaattac tgctgcgaac gaaaacccca tttgatgctg cgaaatggtt aaaaggtgaa
     3001 aatgggatgg ctcggatcac gattgaacgg gaagattcgc aattgggttc tgtctcactt
     3061 atcctttatt ccaatcaaga cattggtgga ggaatctcta cttatgaagt ggtccatcaa
     3121 attgcaaaac gtggaagtaa catagaagca cgggatcgac gttttgacgt caccttatta
     3181 atcaatggat taccgattgt ccaaatcgag ctgaaacaag taactgcaaa agatggtgtt
     3241 tatcaggcat ttaaccaaat caaaaaatat gcagaagaag gtatgtttcg gaacaatatc
     3301 ttttcgacct tacagttgtt tgtggtttcc aatgaacaaa ccactcggta ttttgcaaat
     3361 gccttaccga aagacttgca ccctaagttt ttatttagtt ggcgaacgaa agacaatgaa
     3421 aaagtagaaa acttatatga gttttgtaaa caagtgttaa atatcccgga tgctcatcgt
     3481 ttgattgcgg attatacaat cgtcagtgaa gatcaagaca ataaaaccct catggtgtta
     3541 catccttatc aagtacatgc gattcaagcc ttgtttattg ccgcaaataa acatcaatca
     3601 gggtatgtgt ggcatgcgac tggttcagga aagacgttga ctagttttgt atctactaag
     3661 cttttagcac gaaaatcggg gatcgatcga acgatcatgt tagtggaccg caaagattta
     3721 gataatcaaa ccaccacaga gtttacaaaa tttgcatcgg aatttaatac tgggatttct
     3781 tccgggaacg ccaaagcgaa tagcttgatc gttggaacgg gaagtgccaa agaattaagt
     3841 gaaaccctgt tggcagatgc caatgcaaat gttgtaatca ttacaacccg tcaaaaacta
     3901 gatgcggcgt tgaaatatgc aaagaaacaa gaagagaaaa aaggaaccaa tcgcttccaa
     3961 aaattaatgg gccaacatat cgtgtttgta gtagatgagt gtcatcgagc gttaagtgct
     4021 gaaaatatgg aagaaatcaa aaaaatgttt ccgaaatcca cttggtttgg ctttacaggt
     4081 acaccgattt ttcctgagaa tcggaaacaa gccaaagggc aattggcacg tacaacccat
     4141 gatcagtatg gagaggtctt gcatacctat accattaaga atgcgctaga agatggctct
     4201 gtcttaggat ttcaagtaga gcatgaaaac accgtggagc cgacttcact agagaataaa
     4261 atctatcgga aattaaaaga agtagaaaca tatgcagagt attcatcaga acagatcaat
     4321 cgtatgattg atcaaatgga acctgtcaaa aaagaaagtt atttggaccc agctgttttt
     4381 gaagcagatg aacatatcca aaaagtcatt cataaaatgt tccgtccaga taatgcctat
     4441 acaaaatttg attttcgaaa tggcaggcca acgaaatctg caatcttaac cacgagttcc
     4501 attgacatgg ccaaaaagta ttatcgggcc atcaaagaga tgacgaaaga gcctgattgg
     4561 ttaacaaaag agtttcccaa tcagctcatt cgtgaaggac gaacgatgga ggatcctgat
     4621 tttccacgaa tcgctattac ctattcatta gaagaaaata gccgagattc aagtgcccaa
     4681 caagaagaaa tgcaaaaaat tattgaggaa tataatcagt attatgatac ttcctggtca
     4741 ttagcagaca ttgagcgtta taacggagac atcaataatc gtttggcacg aaaaagagcg
     4801 gaattcaaac aatttgggaa acaaatcgat ttagcgattg tagtggatcg tctattaaca
     4861 ggttttgacg cccctacgat tcaaacccta tttgtggatc gtaatctaga gtatgcagga
     4921 ttgatccaag ctttttcacg tacgaatcgt acgtatcctg ataagacaaa aggattgatt
     4981 gtgacgtttc gtaagccagc gacaatggag aagaatgtcg aggatgcgac aaaactgtat
     5041 tcaaaagcaa aagaagaatc aggtttgatt tatccaagtt atcaggaatc caaaaaaaga
     5101 ttcaaacaag catatggtaa gttaaaagag gtttcatcca tggaatccat tgatgaacat
     5161 acgccacttg aaatccgtat cgagtacgtc aaagcatttc aagaattgaa tcgtgcttat
     5221 gaatcgttag tgacttatga cgattacaat gatgacatgg tgaaatctcc aactttgaac
     5281 aatcaaattc aacttttgga agaacaaatt ggcgtgtacg agaccgtgaa gggttcatta
     5341 atagaggaag aaccagaaaa agaaggagaa gatttttcaa cgatcgaatt ttatagtgaa
     5401 aataccacta agttgtatga tattgattca acttatattg atcaattact aggaacttat
     5461 gctgcgaata gttcagatgc acgagaagaa attgaaaaag ctttggctaa attgaataaa
     5521 acagaaggag tcaaagaagt ttaccgacaa attttaaatg catttgatgt ggggacttta
     5581 gatagtaatg aagatatctt tgtcattaaa cgtcgctatt ttacacaagc gagtgatgat
     5641 ttgattcatg tcttttccaa tgagtggttt gtttctgaga aagaattaca tgcttctaat
     5701 ctgcaatatc taccaggtga agatccaatt cctaatatga aagcaattat caacagtaag
     5761 gattacgaag gatataaagc caaacatcca gaagcgaagc catttaaata cccacaagaa
     5821 atgaagcgag catggcgtaa aatgttagat gatgagttga ttccacttga gaatgaattg
     5881 aggtaaacga gaagagttaa gaaaattgta cgattcaaag atggtagtca agtggaagtg
     5941 tagaaaatag ggatagatgt cttttatttg ttcacatttt ataacgaagt atttaagaca
     6001 gctattgaat aatgtttcct ccaccttttt cattcattta ccccaccccg ttgttatatg
     6061 accgtgtttt gagaggaacc aataaaaagc ataggctaac taggggttcc tttttctttt
     6121 gtttttttgg aatccgctta agtgaaatgt atctgaaatg aaacggatat aaagtgattt
     6181 tttgctataa tgacaaggtt ttagctgttt cagataccat gtaacccaaa atagtttatt
     6241 gagaaaatag gagagtattt gatactaact agtaggaaaa aatgagataa aaattaaatg
     6301 gaatcatttc aggtaatctc aaataaaaaa atatacagtc ctgtggcact ttacctatgg
     6361 gactgtatat tttttactct cagcgggctt gttttgactc cgtgtctgac tccgtcgtat
     6421 tgattttaaa tgatattcga tgaccattta acaggtcatt tttgaaattt ttgtctctat
     6481 atgactcagt ttgataatta ttgatagatc aatttgggtc tatatcgaac aagggaataa
     6541 tccaataaaa aatgatttag aatatcaaaa taacaaaaaa gaggactacc tgtattcgtc
     6601 ctcttttttg actagatttt gaccagtcta atttacattc taatatattc atatgagcta
     6661 attttttgat taaatggtaa gtttttcctt gtttgtttta gtttaaacta gtgtgtaatt
     6721 taaaaatcta tatggaaggg tctgtggggt aatacttaaa taaaggctag gaacctgata
     6781 acat
//
LOCUS       DACDIG010000108         6208 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.108, whole genome shotgun sequence.
ACCESSION   DACDIG010000108 DACDIG010000000
VERSION     DACDIG010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.108"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            333..2279
                     /locus_tag="IV247_002645"
     CDS             333..2279
                     /locus_tag="IV247_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP8778652.1"
                     /translation="MIERHVNLLKLLSRQEDFKPASFFSDKLAVSTKTIYQDIELLED
                     YVVNYQVTIERLPSLGIKLIGDRKNVEHLISSVENYLEKDIYSPEKRRVEIIKEVLFG
                     DKPVSLTHLSQSFMVSKTSLYNDLRIINKIIGSGEVKLQSSAEGISFTGNEQAIQRAI
                     KQLVFYYANDMESKTFFQVLNMLFDSKVISVIHDLLFNDYSELTEKVSDYYVRSLIST
                     LVIQCNRLLKGHHLTVEEEFLFNSIRYMETYIVANGLIEELKAKLNLPFYDADKEYLC
                     RQLFAHRVTNNLKTSNEEYAMSVHKLIERMSEIEKIDLTQNDRLYQSLLYHVPAMVLR
                     LKKGIKIQNPLLENIKSQYSELFSIVWYAMSILEEEFDVTLTDDEVSLILIHFQIAIE
                     DQSKANNIIIICQYGISSAQLIYNKVRRFLPAKDNVEISTLDKFHLAEIGEVDLIISS
                     LSFDAGGIPLVRVSPLVSKTDYMNIMEAYTKYLIEEPSRFIQNELIPKAVKVPTISKF
                     VQSSLVKTGVQVESKEECLDLMISELEANSLVTEEFRASIFSREKMGNTNLESGVALP
                     HANPSMVIESNIFIVALKNAINWGDRKVKLVIMVSLAEEDVSEIRGVYEELYQFIGQK
                     EMVDRLLQVHDSEKLLRIFQQKGE"
     gene            2282..2737
                     /locus_tag="IV247_002646"
     CDS             2282..2737
                     /locus_tag="IV247_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8778653.1"
                     /translation="MYLDKELCLFQLEVKDQKELFQVMSEQLKNAGCVKDSFLEGITN
                     REQEFPTGLEVNQIGFAIPHTDSAHVNSSQICFASLKEPLVFSDMTDKSHEIPVRLVF
                     MLAMSQPHEQIDTLQNLVSLFQNEEKVNELLACTTKEAFIELVHSAGVY"
     gene            2751..4079
                     /locus_tag="IV247_002647"
     CDS             2751..4079
                     /locus_tag="IV247_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP8778654.1"
                     /translation="MDIVLNAFEWFIGLGSNVFLPLIIFVIGILFGLKPGKAFVSGVT
                     VGIGSIGLGLVLSLLSDGLGTAIQQMGEQYGGGLNILDIGVGVGGPLAYSTSLGILMI
                     PISLILNFIFVVLGWTKTLNVDIWNFWFAIFLGMITQSVTGNFWFGLIGAIVAVILQW
                     FLADRTQKTVSEFFGYPGISITHMMGLSGVLFAKPVNWVFDRIPGFNKIDADAETLTK
                     RFGVLGDTVVIGLIIGIVVGIMAGYDVAGIGNLGIMTAAVMKIMPKMVAMFMEGLMPI
                     AEAAKEFTNKKLNGRQVNIGMDAALTVGHPTIMSTSLLLVPISLLLALILPGNKVLPF
                     GDLAFFAFAICLMIPYFKGNIVRSLVGCSLYVVTTLYMATWLAPIITDVFTLASFDVG
                     TAGLVTMLLCGLWPVGLFVLLASYFGVIGIGGLGVVLVGASIYMNKVKKA"
     gene            4113..4397
                     /locus_tag="IV247_002648"
     CDS             4113..4397
                     /locus_tag="IV247_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8778655.1"
                     /translation="MKKLLVICGAGHATSTIAVAKLSKWLEEEGLTDQVKIYQSKIAD
                     ELNKIDEYDAVVSTTIVPDVIKAKVTNGLGLLTGIGTDAIYADVKERLGV"
     gene            4399..4914
                     /locus_tag="IV247_002649"
     CDS             4399..4914
                     /locus_tag="IV247_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HAP8778656.1"
                     /translation="MRFHHDTLTIETVSGRPSYHDIRKELLDTVDTAGIENGMLVISS
                     PHTTCSLFFEETMHDTNFFGDDYLHVDINNVMETIIPKMTSENQYNSPGPKHIDFGTR
                     LSDPNYPAEKWVMLNTDAHLRSSIYGTNSMTLIVQEGKLLLGEMGRVYFVDWDQLRER
                     TRTINLMIMGE"
     gene            4930..5592
                     /gene="rpe"
                     /locus_tag="IV247_002650"
     CDS             4930..5592
                     /gene="rpe"
                     /locus_tag="IV247_002650"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP8778657.1"
                     /translation="MTIAPSLLNAPIAEVKELFEILKEKNVDYLHIDVMDGCFVPDMA
                     FGPSVIKDIRPLTDVVFDVHLMIDTPEKIVTRFVEAGADIVTFHIEATKHAMKVIQLV
                     KEAGAKVGITFNPATPVSFIEPVLSLVDQVLVMTINPGTENKHFIQETVVKIEQLDVI
                     RKQNDYTYDIEVDGKIDNQTIKVCSKAGADIFVSGGFIFNGKNTPQEQIDKLNQSLEE
                     VK"
     gene            5599..6171
                     /locus_tag="IV247_002651"
     CDS             5599..6171
                     /locus_tag="IV247_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8778658.1"
                     /translation="MTNKDQNKNYLEAIILELQNNSKRINSVELEQFSEKIVQAKHIF
                     LAGAGRSGIAMQAFTNRLMHLGFSVSLVGEISSPHSQPGDLLIICSGSGETGSLKNLA
                     AKANQSGVSIALVTMKKDSSIGQLADSVLVLPGITKDENQREEDTFSQPMGSSFEQLA
                     FLTFDSLVLSLMDKTGESSETMFARHADFE"
BASE COUNT         2104 a          871 c         1198 g         2035 t
ORIGIN      
        1 taggactcaa ttgttcatag atgagcattt gtaattaagt tatattataa taaaaaaata
       61 cgaattttag tgacgccatt atattgtttt tatttttcaa ttgtaatatt tcatattact
      121 tccataataa ggagtatttt ctattggaat atcttagctc ttttaaggat ttctcctttt
      181 ttgtttctca ttttttaaga ttatttggat ttcctttaca acaaacttcc acaagtatta
      241 ttgacataga tgtaattgag ggaagggggt tccagtgcta gaataaataa tgaaaagagg
      301 taacaaatgc tagcgttaag aggtgaacaa gtttgattga aagacatgta aatttattaa
      361 aattattaag tcgacaagaa gactttaaac ccgcttcttt ttttagtgat aaattggcag
      421 tgtcgaccaa gaccatttat caagatattg aattgttgga agattatgta gttaattacc
      481 aagtaacaat tgagcgatta ccttctttgg gtatcaaact cataggtgat aggaaaaacg
      541 ttgaacattt gatttcatcg gtagaaaatt atcttgaaaa agatatttat tctccagaaa
      601 aaagacgtgt cgagatcatt aaagaagttt tgtttggtga taagcctgtt tctttaactc
      661 atttgtctca aagttttatg gttagtaaaa catcgttata caatgacctg agaattatca
      721 ataaaattat tggcagtgga gaagttaagt tgcagtcatc tgcagaagga attagtttta
      781 ctggtaatga acaagcaatt caacgagcaa tcaagcaact tgtattctat tatgcgaatg
      841 acatggaatc aaaaacattt tttcaagttt taaatatgtt gtttgattca aaagttatta
      901 gtgtcattca tgatttgcta ttcaatgatt acagtgaact gactgaaaag gtttctgatt
      961 actatgttcg gtcattgatt tctactttag ttatccaatg taaccgttta ctgaaaggtc
     1021 atcatttaac tgttgaagaa gaattcctct tcaatagcat tcgctatatg gaaacgtaca
     1081 ttgtggcaaa tggtttaatt gaagaattga aggcaaaatt gaatcttcca ttttatgacg
     1141 cagataaaga atatctatgt cgtcaactat ttgcacatcg tgtgacaaat aatttaaaaa
     1201 ccagcaatga agagtacgct atgagcgttc ataaattgat tgaaagaatg agtgagattg
     1261 aaaaaattga tttgactcaa aatgatcgat tatatcaatc cttattgtat catgtgccag
     1321 caatggtcct tcgtttaaaa aaaggtatta aaattcaaaa tccgttatta gaaaatatca
     1381 aatctcaata ttcagaactc tttagcatag tatggtacgc catgtccatc cttgaagaag
     1441 aatttgatgt gactttaacg gacgatgaag tttccttaat tttgattcat tttcagattg
     1501 ctattgaaga tcagtccaaa gcaaataata tcatcattat ttgccaatac ggtatttctt
     1561 ctgcgcaatt aatttacaat aaagttcgac ggtttttacc agcaaaagat aatgtagaaa
     1621 tatcaacttt agataagttt catttagctg aaattgggga agttgatttg attatttcct
     1681 ctctttcctt tgatgcaggt ggtataccct tagtaagggt atctccattg gtaagtaaaa
     1741 cagattatat gaatattatg gaagcttata ctaaatattt aatagaagaa ccttcgcgat
     1801 ttattcaaaa tgaactgatt cctaaagcgg ttaaagttcc caccatatct aaatttgttc
     1861 aatcatcttt agtaaaaaca ggagtacagg tggaaagcaa agaagaatgt ttggatttga
     1921 tgatttccga actagaagcc aattcattag ttactgaaga gtttcgtgct tctatcttct
     1981 ctagagagaa aatggggaat actaatttag aaagtggtgt tgctttaccg catgcgaatc
     2041 catcaatggt cattgaatcc aacattttta ttgtagcatt aaaaaatgcg attaattggg
     2101 gcgatcgaaa agtcaaatta gtcattatgg ttagtttagc tgaagaagat gtatctgaga
     2161 ttagaggagt atatgaagaa ctttaccaat ttattgggca aaaagaaatg gtcgatcgct
     2221 tgttacaagt tcatgatagc gaaaaattac taagaatttt tcaacaaaaa ggagaataaa
     2281 aatgtatcta gataaagaat tatgtttatt tcaattagaa gtgaaagatc aaaaagaatt
     2341 gtttcaagta atgtctgaac aattaaaaaa cgctggttgc gtgaaagata gtttcttaga
     2401 aggtatcacc aaccgtgaac aagaatttcc aactggatta gaagtaaatc aaattgggtt
     2461 tgctatccca catacagatt ccgcacatgt aaatagctct caaatctgtt ttgcctcatt
     2521 aaaggaacca ctggtatttt cagatatgac agataaatct catgaaatac ctgtacgttt
     2581 agtctttatg ttggcaatgt cacaacctca tgaacaaatt gatacactac aaaacttagt
     2641 tagcctattt caaaatgaag aaaaagtaaa tgaattactt gcatgtacca cgaaagaagc
     2701 atttattgag ttagtacatt ctgcaggagt ttattaggag gaatagagag atggatattg
     2761 tattaaatgc ctttgaatgg tttattggat tagggtcaaa cgtcttttta ccactgatta
     2821 tttttgtaat cgggatatta tttggattaa aacctggtaa agcatttgtt tccggcgtta
     2881 ctgttggtat cggttctatt ggtttaggat tagttttaag tttattatca gatggtttag
     2941 gaacagcaat tcaacaaatg ggggaacaat acggtggtgg cctgaatatc ctagatattg
     3001 gcgtaggtgt tggtggacct ttggcttact ctacatcatt gggaatttta atgattccaa
     3061 tttcattaat tcttaacttc atatttgtgg ttttgggttg gacaaaaaca ttgaatgttg
     3121 atatttggaa cttttggttc gcaattttcc taggtatgat tactcaatct gttacgggaa
     3181 acttttggtt tggcttaatt ggcgcgattg tcgcagtaat tttacaatgg ttcttagcag
     3241 atcgtacaca aaaaactgtt agcgaatttt tcggctatcc aggaatctct atcacacata
     3301 tgatgggatt atcaggagta ctatttgcca aaccagtaaa ttgggttttt gatcgtattc
     3361 ctggatttaa taaaatagat gcagatgcag aaacattaac aaaacgtttc ggtgttttag
     3421 gtgacacagt tgttattggc ctaattatcg gtattgtagt gggtattatg gcaggctatg
     3481 acgtagcagg aattggaaac ttaggaatta tgactgcggc agtgatgaag attatgccta
     3541 aaatggtagc aatgtttatg gaaggtttaa tgccaattgc agaagcggcg aaagaattta
     3601 cgaataaaaa gttaaatggt cgccaagtaa acattggaat ggatgcagcg ttaactgtgg
     3661 gtcatccaac tattatgtca acttctctat tgcttgtacc aatctcatta ttgctagcac
     3721 taattcttcc aggaaataaa gtattacctt ttggagattt agcattcttt gctttcgcca
     3781 tctgtttaat gattccttat tttaaaggaa atattgtgag atcattagta ggatgttcgt
     3841 tgtatgttgt tacaacatta tatatggcaa catggttggc accaattatc actgatgtgt
     3901 ttacattagc aagtttcgat gtggggacag caggattagt aactatgttg ttatgtggat
     3961 tatggccagt agggttgttt gttttattag catcttactt tggtgtaatt ggaataggtg
     4021 gattaggagt tgttttagta ggtgcttcta tctacatgaa caaagtgaag aaagcttaat
     4081 cataaaatta aaataagaaa gaagagatca atatgaaaaa attattagtt atttgtggag
     4141 caggtcatgc tacttctaca attgcagtag caaaattatc aaaatggttg gaagaagaag
     4201 gattaacaga tcaagtgaaa atttatcaat ctaaaattgc tgatgagcta aataaaattg
     4261 atgaatatga tgctgtagta agtacaacaa ttgtccctga tgtaattaaa gctaaagtaa
     4321 cgaatggctt gggcttatta acaggtattg gtacagatgc catttatgca gatgtaaaag
     4381 aaagattagg tgtttaatat gcgttttcat catgacactt tgactattga aacagtttca
     4441 gggcgtcctt cgtatcatga tattcgaaaa gaacttttag atacagttga tacagcaggt
     4501 attgagaatg gaatgttagt aatttcgagc ccgcatacaa cttgctcact cttttttgaa
     4561 gaaacgatgc atgacacaaa cttttttggt gatgattatt tacatgtaga catcaacaat
     4621 gtaatggaaa cgattattcc aaaaatgacg agtgaaaatc aatacaacag tccggggcca
     4681 aaacacattg attttggaac aagattatct gatcccaatt accctgcaga aaaatgggtc
     4741 atgctaaata ccgatgcaca tttacgttca tctatttatg gaaccaactc aatgacttta
     4801 attgttcaag aaggtaaact tttattagga gaaatgggcc gtgtctattt tgtcgattgg
     4861 gatcaactaa gagagcgaac tagaacaatc aatctcatga ttatgggaga ataaacgggg
     4921 gaatagagaa tgacaattgc tccatcattg ttaaatgcac ctatcgcaga agttaaagag
     4981 ttatttgaaa tcttaaaaga aaagaatgtt gattatttgc atattgatgt aatggatggc
     5041 tgttttgtac cagatatggc ttttggacca agtgttatta aagatattcg tccactaaca
     5101 gacgttgtgt ttgatgttca tttgatgatt gatacaccag aaaaaattgt cacaagattt
     5161 gtagaagcag gagcagatat tgttaccttc cacattgaag ctaccaaaca cgcaatgaag
     5221 gtcattcagt tagtcaagga agcaggagca aaagtaggga ttacttttaa tcctgcaaca
     5281 ccagtcagtt ttattgaacc agtattatct ttagtcgatc aagtattagt aatgacaatc
     5341 aatccaggaa cagagaataa gcattttatt caagagacag tagttaaaat cgaacagtta
     5401 gatgtaatac gcaaacaaaa tgattatacg tatgatattg aagtagatgg caagattgat
     5461 aatcaaacga ttaaagtttg ttctaaagca ggggcggata tttttgtatc aggcggtttc
     5521 atcttcaatg ggaaaaatac cccgcaagaa caaattgata aactaaatca atcattagag
     5581 gaagtgaagt aaaagatcat gaccaataaa gaccaaaata aaaactattt agaagcgatt
     5641 atcttagaat tacaaaataa ctcaaaacgt attaattctg tagagttaga acaatttagt
     5701 gagaaaattg ttcaagctaa acacatcttt ctagctggtg caggacgctc tggaattgcc
     5761 atgcaagcat ttacgaatcg cttaatgcat ttaggttttt cagtcagcct tgtcggtgaa
     5821 attagttcac cacattcgca acctggagat ttattaatta tctgttctgg ctcaggtgaa
     5881 acaggtagtt taaaaaattt agcggccaaa gcaaatcaaa gtggtgtaag cattgcttta
     5941 gttaccatga aaaaagactc gtcgattggt caattagcag attctgttct tgtattacca
     6001 ggtattacca aagatgaaaa tcaacgagaa gaagatactt tttcacaacc tatgggttca
     6061 agttttgaac aattagcatt tctcaccttt gatagtttag tgttatcttt aatggataaa
     6121 acaggtgaaa gctcagaaac gatgtttgct cgacatgcag attttgagta gtagatttta
     6181 aagataaaaa accattctct aaatgcta
//
LOCUS       DACDIG010000109         6173 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.109, whole genome shotgun sequence.
ACCESSION   DACDIG010000109 DACDIG010000000
VERSION     DACDIG010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.109"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            725..811
                     /locus_tag="IV247_002652"
     CDS             725..811
                     /locus_tag="IV247_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP8778659.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="IV247_002653"
     CDS             complement(932..1621)
                     /locus_tag="IV247_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778660.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="IV247_002654"
     CDS             complement(1628..1906)
                     /locus_tag="IV247_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778661.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="IV247_002655"
     CDS             complement(1866..2780)
                     /locus_tag="IV247_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP8778662.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="IV247_002656"
     CDS             complement(2762..3142)
                     /locus_tag="IV247_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP8778663.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="IV247_002657"
     CDS             complement(3592..3879)
                     /locus_tag="IV247_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP8778664.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="IV247_002658"
     CDS             complement(3899..4123)
                     /locus_tag="IV247_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HAP8778665.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4763..5296)
                     /locus_tag="IV247_002659"
     CDS             complement(4763..5296)
                     /locus_tag="IV247_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP8778666.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="IV247_002660"
     CDS             complement(5317..6159)
                     /locus_tag="IV247_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP8778667.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DACDIG010000110         6045 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.110, whole genome shotgun sequence.
ACCESSION   DACDIG010000110 DACDIG010000000
VERSION     DACDIG010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.110"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(117..1079)
                     /locus_tag="IV247_002661"
     CDS             complement(117..1079)
                     /locus_tag="IV247_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8778668.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1081..2520)
                     /locus_tag="IV247_002662"
     CDS             complement(1081..2520)
                     /locus_tag="IV247_002662"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAP8778669.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2705..4651
                     /locus_tag="IV247_002663"
     CDS             2705..4651
                     /locus_tag="IV247_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAP8778670.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            4915..5787
                     /locus_tag="IV247_002664"
     CDS             4915..5787
                     /locus_tag="IV247_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8778671.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT         1910 a         1038 c         1069 g         2028 t
ORIGIN      
        1 tctaactttt gggggtcact acaagttgac aactcctttt tgtttacaaa tcaattggat
       61 atctacaaat tgtattttct aacgaatttt atagacatta aaaaattaat ttaacgttaa
      121 atacttcctc cagaaagtat tgagattggt aaaatataat ccttatttgt cttttcgcct
      181 ttaatttttt tcagaagaat atcaacaata agacttgcaa tttcatcaat tggttgtctt
      241 attgttgtca attgtgggaa aaatttctca ataaaagagg taccatcgta tccaataatt
      301 tttaaatctt ctgggatatt aagtttaagt tgatgagcaa ttttcatagt gagaatggct
      361 gttaaatcat cagaaacaaa aataccatct ggtttgtgat ttataatagt tgactttatt
      421 tccatttcac gacggatagt agacagatta ttaggaattt tgaaaatttt tccatttgga
      481 ttttgaaaag aaaaacctag tgcacgtaat tctgtgggag aatccgtgtt atcatttcct
      541 gtaatcataa tggtattttg acaaccattt ttttgtaaag tcttagctgc aagttttccc
      601 ccttcaaagt tatctgaaga aacaatggaa acattaggag ctaaatttct atcaaaagca
      661 acaataggtg ctctcacacg ctcataatca gcaattccaa gattatggct tgatgagatt
      721 atgccatcaa cttggttggc ttcaagcatt tcaatgtatt ctctctcttt agcaggatca
      781 ttttgactat tgcaaataat cgttttatat ccatgtttaa ataactctat ttcgagatac
      841 tcaatgagtt cagagtaaaa gatattacta atattaggaa aaataagacc aataagctga
      901 ggtgactttc cttgaagtcc acgtgctaaa ttatttggtt tataaccaag agttttcata
      961 gcttcatgaa ctttttgttt tgttttttct gataaatatc ccttattatt aatgacgcgt
     1021 gaaacggttg ttgggctaac tccagctaat tcagcgacat cagttaattt tgctaccatt
     1081 ttaatatttt aattcaaagt attttccagt aggttcgcct gatttgattt gaataccatt
     1141 ttggtgattt tcagggaata cacgactagt aaacactttt tctcctttat taataaaaat
     1201 ctcaatgatt gagttatcta caaaaatgtt agcagttgtt tctttagtat caattgagca
     1261 ttcacgaatc gtaccaaagt cagtggcata ttgaatgcct gctttgctgc gatctaaaat
     1321 aatttttcct tctttggtat ctacaatcaa gctaagtcca tttcctttac tatcagagaa
     1381 aagaagtaac tccgatttct gatttggagg aaaagttagt tcaagctcat aagtattatt
     1441 tgtttctgct tttgctgtaa attgttcaga actagcacgt aatgatgtaa tagcttctac
     1501 tggatattgg taaagtttac catcttttat tgaaagttct tttacaagac tcattgctcc
     1561 ctgataatca tacttatcag ttggataatc gatatcaggt agtccaatcc aactgacaat
     1621 cagagcacga ccatcaggag tattgaatcc ttgagtcgca tacgcttcaa atccataatc
     1681 aagattttga atttctgaag cgcccacaag ttttactttt tctatgtcaa gcgattgaca
     1741 gatcttaaaa gtattcggat agatattccc ataatttagc tcagatttat ccaacccttg
     1801 aggacaataa atgagaacag gttttttgtc tacaaatact agatttggac actcaatcat
     1861 gtattctgaa ccagtgccgc caaaatcaag gttgccaact tcctcccatg tttccacgtt
     1921 attgtcatcc gctttgtaaa gtttgataaa gcctgatttt tctaaatttt gtgctccaac
     1981 aatcgcatac aattgtcctt cataattaaa catttgtgga tcacgaaaat gatcagttac
     2041 atcagcaggt ttatgaataa gaacattctc acctttcgta atgttgtagt ctttatccat
     2101 ccatgctccg atttgaagtg tatcacgttc ccaattttca tttcgaacgt ttcctgtata
     2161 gagtaagaat agattcccct ctatttcata tgcagaaccg gaataagcgc cgtggctatc
     2221 tcgttttgtg tctggaagaa gttctgctcc tgttacatga aaatgaacta aatcatctga
     2281 ttcattgtgt acccattctt tcaacccatg agcagctcca aatggccaat tttgataaaa
     2341 aagcgtatat tttccgttaa aaaaagaaaa accattagga tcgtttaaca atcctgtttt
     2401 aggttcgata tgatagctag cacgccaagg tgatcgtttt acattatcct taattttatc
     2461 catctcatct ttagtccaat cagaataagc acgatatcgt atttctcgag gtaagttcat
     2521 attttactcc ttcaatttat ctttttacaa ctagtatggt aaacgttttc cgttgaaaaa
     2581 tcaatcattt catttaaaaa aataaactat atgacaaacg tttgacatat ttttaagata
     2641 tgctaaaata aatttgcatt tcagtgaacc gttttcacgt aaaaataaaa aaggagtgat
     2701 tcgaatgaat tacagtaaag tagcttccga cgtaatacaa gcggtcggaa aagataacct
     2761 tattgcagct gcacactgcg ctacacgtct tcgtctcgta ctaaaagatg acacgaaagt
     2821 gaatcaaaaa gctttagacg aaaatccaga tgtgaaaagc acatttaaaa ttgatggaca
     2881 atatcaagtg attatcggtg caggtgatgt taatttcgtt tatgacgaac ttatcaaaaa
     2941 aactggactt tcggaaattt caacagatga tttaaaacaa attgctagta aagataaaaa
     3001 attcaacccc attatggcat taattaaatt attatcagat atttttgttc caattatacc
     3061 tgcccttgtt gcaggtggtt tattaatggc attacaaaac tttttaacag ctgctggact
     3121 ttttggacca aaatcaattg aagaaatgta tcctgcgatt gaaggtattt cctcaatgat
     3181 ccaactgatg tccgctgcac cattcatgtt cttaccaatt ttagtaggta tttcagctgc
     3241 taaacgtttt ggagcaaacc aatttcttgg tgctgcaatt ggtatgatca tgacaacacc
     3301 agatcttggt ggctcaactg aatattggaa tatttttggt tatcatgttt cacaaacaaa
     3361 ctatgcttat caagttattc cagttttagc agctgtttgg atattatcta tattagaaaa
     3421 attctttcat aaaaaattgc catcatctat tgactttaca ttcacgccat tattatcagt
     3481 aatgattact ggatttttga ctttcacagt tattggccct gtaatgttat tgttgtctaa
     3541 tggaattaca gacattattg tttggctata taacactact ggatttattg gaatgggtat
     3601 ttttggagga acatattcac ttatagtcat gactggttta catcaatcat ttccagctat
     3661 tgaaactcaa ttactttcag cgtggacaaa tggtatcgga catggagact ttatctttgt
     3721 agttgcatct atggcaaacg ttgctcaagg tgctgcaaca tttgccattt ggttcttaac
     3781 taaaaattct aagacaaaaa gtcttgcttc ctctgcgggt gtctcagcac ttcttggaat
     3841 tacagagcct gccttgttcg gagttaactt aaaataccgc tttccattct tttgtgcttt
     3901 aattggttca ggaattgcta cagcagtagc aggattactt aaagtagttg ctgtttcact
     3961 tggttcagca ggtttcttgg gatttctttc aattaatgca acatctattc cattttacgt
     4021 attgtgtgaa ttgattagct ttgtagttgc ttttgcaatt acttacttct acggtaaaac
     4081 aaaagctgct ggtatcttta aagcagaaga aattgctgaa caaacaccag ttaattcttc
     4141 aaaagtagca acaacagatc caatatctac tgatgttgaa tcaacacaaa caaaagtttc
     4201 atcagaaaca attgttagtc ccatttctgg taaagtgatt agtctttctt cagtaaatga
     4261 tccagtcttt tcatcagaag ctatgggaaa agggattgcc attaaaccaa atggaaacac
     4321 ggtccattct ccagtagatg gtactattca actcacgttt gaaactggac atgcttatgg
     4381 tattaaatca aataatggtg ctgaagttct aattcatatt ggtattgata ctgtatcaat
     4441 gggaggaaaa ggatttacac aaaaagttac tactaatcaa aaagttagaa aaggcgatat
     4501 tcttggagca tttgaccgtt caatgattga gaaagaaggt cttgatgata caacaatgat
     4561 tattgtgacc aatacttcgg attattcaga agtaaatata gtagctgaaa atacagtcac
     4621 tgagggtact gatttacttg aaatcaaata aatatatttc cctgagtaaa gatggaattt
     4681 tctttccaaa atcaaataat atagattaat caaatatttg agaataacat tttatttaat
     4741 cttttcatat atcaattgta tgaaaaatta ttagaatttt aaaaaatgat aaaaattcaa
     4801 aaagaaaaaa tagtagttat ttacttgtca tgttactaaa taaaaaaata atttaaatat
     4861 gatatatagt catttattta gtgatttcat tctaaaataa agggagaaat gtctatgcta
     4921 tacggtgcaa ttgaagcagg cggcacaaaa tttgtgtgtg ccattggcga tgaagaaatg
     4981 acgatcaagg aaagagtgag ctttccgaca acgacacctg aggaaacaat gcctttagtc
     5041 attgacttct tcaaacaata ccaagcagat ttagcaggga ttgggattgg ctcatttggg
     5101 cctatcgata tccatcgcga ttccgccact tatgggtaca ttacctcgac cccgaaattg
     5161 gcatggcaaa attttgattt catcggtacc atgaaaaaag aatttccgat cccgatttct
     5221 tggacaacag atgtcaatgc ggctgcctat ggtgagtatg tcttcggcag tggcaaagga
     5281 ttgagcagtg tcgtttacta tacgatcggt acgggtgttg gcggcggtgc cttacaagaa
     5341 gggcgcttta tcgaagggtt tagtcatcca gaaatggggc atatgctcgt cgtccctcat
     5401 ccaaaagatg actttgctgg cagctgtcca tttcatggca actgtttaga agggatggcc
     5461 gctggacccg ccatcgaaaa ccgcttaggg aaaaaaggac aagaggttgc agaagatgat
     5521 ccttattggg aaatcgaagc ctcctatatt gctcaatgtg cctacaatac aaccttgatg
     5581 ttttctccag atgtgatcat ttttggcggt ggtgtgatga aacaacgcca tatgctgcaa
     5641 aacgtccacg acgcatttgc aaaattggtc aatgggtatg tcgaaacacc tgagttaagc
     5701 aaatatatcg tgaccccagc attagaagac aacgctggaa cgctcggttg tttggcattg
     5761 gctcgagaag cggtcttaca ttcataaacg aaaaaaggct cttcgacatt tagtgttggt
     5821 agagaaagat acaactgaaa atttagtcat aaccagttag gcagagtgct taactggttt
     5881 ttgttttact tggaagatca agccttgaat gagataaatt cttgctcgaa agttatggaa
     5941 atttcgatag ccgtaggcca cttgtattgt gcaatagttt tttacaaaaa aaagggggtg
     6001 cggatatttt tttggacctt tttaacaaat atatctttgc ttctt
//
LOCUS       DACDIG010000111         5989 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.111, whole genome shotgun sequence.
ACCESSION   DACDIG010000111 DACDIG010000000
VERSION     DACDIG010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5989
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.111"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(201..686)
                     /locus_tag="IV247_002665"
     CDS             complement(201..686)
                     /locus_tag="IV247_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778672.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(825..1040)
                     /locus_tag="IV247_002666"
     CDS             complement(825..1040)
                     /locus_tag="IV247_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAP8778673.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            1264..2064
                     /locus_tag="IV247_002667"
     CDS             1264..2064
                     /locus_tag="IV247_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8778674.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            2083..3951
                     /locus_tag="IV247_002668"
     CDS             2083..3951
                     /locus_tag="IV247_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8778675.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            3944..4435
                     /locus_tag="IV247_002669"
     CDS             3944..4435
                     /locus_tag="IV247_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAP8778676.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            4423..4977
                     /gene="srlA"
                     /locus_tag="IV247_002670"
     CDS             4423..4977
                     /gene="srlA"
                     /locus_tag="IV247_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAP8778677.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            4995..>5989
                     /locus_tag="IV247_002671"
     CDS             4995..>5989
                     /locus_tag="IV247_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAP8778678.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2100 a          954 c         1209 g         1726 t
ORIGIN      
        1 actcagttga ccgaaaatga agtagatacc aaaaatttat caagagagca agtagagaag
       61 tgagtatatt cataagagaa atagctgtaa cgaattatta ggttaatgca aatgcttatt
      121 tatactccgt ataactttct cagaaaaaat gataccaata atgacttgct agtactgtaa
      181 atccgtataa tatcatctaa tcaaaatggt gttccatttg catctgtcat cgtaatcgat
      241 ccttcgggtg acactcgata aaaaccaacg cttccagatc ctccttggga acggatttgt
      301 ttgctggaag ctgtgaacaa ataatctcga tcaatttttt gataaaagct aataacaata
      361 tcgtcacttt gtaattcgga acggtgggca ataagaaata gaagtgcttt gtcagagttt
      421 tctaatggaa gagaaggtac agtgctttct tgcatttcag atgttgaaga aatgctaaac
      481 gactgaatac ttgattggct atgttgcgaa tctacattac cagtagaagt attagtaatg
      541 gaagtagtag aagtactttt agttgaacca acctcagttg ttgaaatggc tgctgaagat
      601 tctttaacag aattctttgt tttatatcca caggcagaaa aaagtattac agttcccaac
      661 aagcctgcgt aaatcccttt atgcatgata gctcctccta aaatagatta tctttattca
      721 gtatatcact taaacaaaga aaagcagatg gtttaaaatg aaaaaacctt cccaataaag
      781 ggaaggttca tgaagataat aactttttta ataattatag ctttctaata tctttcatca
      841 acttttctag taatcattgg gaaaaatttc aagcagtaga tcgcacaaat acctacgacg
      901 atataaatga tcttcgcaaa aatagttgtt gaaccaccag caatcatagc tactaaatca
      961 agttcgaaca agcctaccaa cagccagttc aaccctccca caatcaataa agttaatgca
     1021 atagcatcta aagttttcat tagaattctc ctttcttaaa tgataaaaca attatatatc
     1081 aaaaagagaa acattgctta tataacgctc aggaatttta gcaatctatg caaaaagatg
     1141 aaaatttttt ttgctcttcc attagtacac attttttagc tgaagctgta aatttcatgt
     1201 cttaattttt tttagtgagc ttttatactg gatttgtaag aaaaaagaag gagaggatca
     1261 catatgtcgg attggttgga tattgcagga aaagtagtaa ttgtaactgg aggttcctca
     1321 ggaattggaa gaagtatcgt cgaaaatttg ttgaagcaga atgcacaagt agcgaatttt
     1381 gatgtaactg aatgccgtat acagcatgaa aacctactat ctttaaaagt cgatgtgtct
     1441 tctaaaacgg acatagaaga agggatatat aaagtaatga aacatttcaa gacgatcgat
     1501 ggtctagtca ataatgcagg aataaatatt ccttctcttc taatagatag aaatcatcca
     1561 aaaagcaagt atgaattatc agaacaagtt tttgataaga tgatcgctgt caatcaaaaa
     1621 agtgtatatt tgatgagcca agcagttgga agaatcttgg tgcaaaaggg atcaggagtg
     1681 atcgtgaatc tttcttctga atcaggattg gaaggatcag aaggacagag ctgttatgct
     1741 gctacaaaag cagcgatgaa cagttttact cgttcttggg caaaagagtt aggaaagagg
     1801 aatgttcgtg tggtaggtgt tgcaccaggg atactggaag aaaccggttt gagaacacaa
     1861 gaatatgaag aagctctttc ttatacgaga ggtataagcg tcgaacagct gagaaacggt
     1921 tacagtcgaa caagtacgat ccctctagga aggagtggta agctgcaaga agtggcggat
     1981 ctagtctgct attatctttc agaaagatca agttatatca ctggagtcac gactaatatt
     2041 tcaggaggta aaacaagagg ataaaggata ggggggacgc acgtgacgct agtaaacaga
     2101 tggtaccaat tattgaaatt actagtcact cacaagaaag tttcaatcca ggaagtgcaa
     2161 aaagaaatca aaaccagtaa tcaaaccata aaaaaagata ttggggagtt gaatgaagaa
     2221 attgccggta ttgcaaaaat catccaaaaa aacaaacatt ttgaacttga aatttatgat
     2281 tttgattttt ttgatgagat catgcaagga aaattgaaaa cagccagtga tttcaattcg
     2341 gcatccaaaa gaatcgctta tttactaaat aggctgatcg aaacagagga tttcttacgt
     2401 atggatgatt tgtctgaaga agtcggtgtc agcagaggaa cagtggtcaa ggatctaaag
     2461 acattaaaag agatgatcaa tgattttgat gtaaagatta caggcacacc aaataaagga
     2521 ctaagaataa taggagatga gctagaatta cgactacttt tattttattt tgtttctccc
     2581 tacttttcag agacgaaaac agaacagttt ctagaagaaa aacaagcaat caaacatttt
     2641 cagaagaaga cgaatgctag taaacaagaa atcacccttt taacgaaagt tatcggtatt
     2701 tctttgaata gaatctctag taactatctg ttgaagaagc ccatcaatcg ttacagtggg
     2761 tgttttgaat atatccaaga attcaatgaa ctgatcaatc agttggaaga aatctatgaa
     2821 ctaactttga gccaatatga aaaagatttt atcagttttc ctttgaacat catcaacaca
     2881 agtataacga tgataaggaa agctaactct gatttgcgct tatatttcga ccccatgatg
     2941 atgcgtattc gacgattaga tctcattgat ctggatgagt ccttcttata tcaagagatg
     3001 aaacagcacc tctctttatt gataaatcgg gtaatttttc gttatcgatt gactgattta
     3061 ttttatggtg agatagaaaa aaagtatcct ttttcttacc aaatcgctag cgaatgcata
     3121 caagcattag aagctatctt gcaacataag atctctaaag tagagatcag ctacctaacg
     3181 ttgtatttgg agttagcatt tcgatatcaa aaagagcatt tttcctctaa aaaagtagcc
     3241 attgttcata cgacaggaaa aggaacagca ttaatgatcc aaagacaaat caaaaggata
     3301 ttggggaggg atatacaaat aacacatgtt tcagaagaac aatatgaaaa agtagatcta
     3361 aacaaatatt tcgctgtttt tacaacgatt ccgttaaaaa atgtaaatga agagacaccg
     3421 gtgatccatt tgactaatct ttttgatgac tcttggttaa gggcacaatg gcaaaaagca
     3481 aaaaaaaagc gattagccgg gtttcattct actgtttttt tctatcaaat atggaaagaa
     3541 aaaaagacgt atcaagaata tttaaacaaa ttagtcgctc ctttgatcag aggctattat
     3601 gtggatgaag aatttgaaaa aaggatcttg aaaagagaat atcagcaaag cacatttgtt
     3661 aacaaagaat ttgcttttcc tcacacaacc aaccagctat ctcaacacat tgttttatca
     3721 gtagggctaa taactgatca aggtttagca gaaacaggag gaaatggagt tattttttta
     3781 tttgccatac ctgaagaaat gacggctgaa gttgagaatg aattatttga gctatacgat
     3841 atcctatttg aactcataaa aatgggaata gatgagttta ttgaattagc accgacacaa
     3901 gaatcatttt taaaactaat caaaagtaaa ggagagagac ttcatgaatg atatgtatct
     3961 attcatagtt ttcgcagctt cagcttatgt gctgcaagca gttatcagtt ggaagcaact
     4021 aaagcatttc aatcaaacat atagcgaact gcgaagaaaa ggaaaagtag cgattggtaa
     4081 acgacctggg aaaatccgtt caggcacgat tgttatgcta gctatagacg atcatgatca
     4141 agttttagaa gtcagaaaaa tgcaaggggt tacatctttg gcaaaatttc attcattacc
     4201 tgtctatgcg ggtgaagata tccattacat cgatcgttac catccctcag ttcgagcgga
     4261 agatcgtttg acacaagcag ctttagaaaa tgccagagaa gtatacttac gaatagaggc
     4321 aggaaatgaa aaaagtgaac aaccttcatt gccttttatt gctatgctca ataattttcg
     4381 gatatggaaa agtacgatca aagaaaaaag gagtgacaaa cgatggatat cataacaaaa
     4441 tttgctgagg gatttatgag gctgttccaa acaggtgcag aaacattcat aagctggatg
     4501 acaggaatcg taccagttgt cttgatgctg atggttgcaa tgaatacgct gattgctttt
     4561 ttaggtgaag accgagtaac gaaagtagcc aaattatcag cgaaaaatcc gcttactcgt
     4621 taccttgtgt taccgttctt atctgctttt atgttgggaa atcctatgtc atttacaatg
     4681 gctcgtttcc ttccagaata ctacaaacca agctattatg ctgctcaagc tcaattttgt
     4741 catacgagca atggtgtatt cccccatatt aatcctggag aattgtttgt atggatgggg
     4801 attgctcaag ggatcgaaac cttagggcta aattcgatgg agctggctat ccgatacttg
     4861 cttgtgggac tgctgatgaa ctttattggt ggttggataa ctgattttac tactggattt
     4921 gtatgcagac agcaaggaat cgttcttagc aaaaaagtag agctttcagt tgattagaga
     4981 aaggagagtt ttaaatgtat ggtgatatta agatcgtaag aggaaacggc gggtatggag
     5041 gtcctttgat tattaggccg acaaaagata aacacaagtt catctatgtc acaggtggag
     5101 gtgagaggcc aatgatcgta gataaaatag aagaactgac aggtatggag tctatcaatg
     5161 ggttcaaaac atcaatacct gatagtgaaa tcgcgcttgc aatcgttgac tgtggaggga
     5221 ctcttagatg tggaatttat cctaaaaaag ggataccaac catcaatatc gtttccacag
     5281 gaaaaagtgg cccattagca caatatatca ctgaagatat ttatgtttca gcagtagggt
     5341 tagaacaaat acagccacta atggaaactg atgataacaa agaaacagta aatactgcat
     5401 ctccagataa aaagtacaaa tatgatactt ctaaaaagat aaccgagcaa aaagcagagc
     5461 agcaaaactg gatagcaaaa attggaatgg gcgcgggaaa agtagttgct acattcaatc
     5521 aatcagcaag agaagcagta gatacgatga tccggacagt gatccctttt atggctttcg
     5581 tttccctttt gattggtgtg atacaaggtt ctgggatcgg taatgtattt gctaacatta
     5641 tggcaccttt agcaggaaat gcagttggat taacaatcat tggctttatt tgttcactgc
     5701 catttttatc tcctttgctt ggtccagggg cagttattag ccaagtgatt ggaacattga
     5761 tcggtgtaga gattgggaaa ggaaatattc ctccccaatt agcattaccg gcactatttg
     5821 ctatcaatac tcaaaatgca tgtgatttta ttccagtcgg tctaggtttg gaagaagcaa
     5881 aagctgagac agtagaagta ggtgtaccat cagttcttta ttcaagattt cttaacggca
     5941 tcccacgagt tatcattgct tgggtagcaa gttttggatt atatagctg
//
LOCUS       DACDIG010000112         5981 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.112, whole genome shotgun sequence.
ACCESSION   DACDIG010000112 DACDIG010000000
VERSION     DACDIG010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5981
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.112"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            64..582
                     /locus_tag="IV247_002672"
     CDS             64..582
                     /locus_tag="IV247_002672"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP8778679.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            710..1393
                     /locus_tag="IV247_002673"
     CDS             710..1393
                     /locus_tag="IV247_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAP8778680.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1702..1775
                     /locus_tag="IV247_002674"
     tRNA            1702..1775
                     /locus_tag="IV247_002674"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1736..1738,aa:Arg,seq:tct)
     gene            1934..2293
                     /locus_tag="IV247_002675"
     CDS             1934..2293
                     /locus_tag="IV247_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAP8778681.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2416..4047
                     /locus_tag="IV247_002676"
     CDS             2416..4047
                     /locus_tag="IV247_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAP8778682.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4109..4750)
                     /locus_tag="IV247_002677"
     CDS             complement(4109..4750)
                     /locus_tag="IV247_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP8778683.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            5032..5340
                     /gene="rplU"
                     /locus_tag="IV247_002678"
     CDS             5032..5340
                     /gene="rplU"
                     /locus_tag="IV247_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAP8778684.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5367..5711
                     /locus_tag="IV247_002679"
     CDS             5367..5711
                     /locus_tag="IV247_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAP8778685.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5724..>5981
                     /gene="rpmA"
                     /locus_tag="IV247_002680"
     CDS             5724..>5981
                     /gene="rpmA"
                     /locus_tag="IV247_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAP8778686.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKK"
BASE COUNT         1868 a         1000 c         1277 g         1836 t
ORIGIN      
        1 tgaaaaaaat ttatcgttat tatatgatta agaaggattg aataaagaaa gaagttgaaa
       61 acgatacctg attacaaaaa aataactttt ataagttcta taacgttgat tatattttta
      121 attcttattc ttctcgtcgt cttggttccc cgaatttttg gtttgactcc ttatattatt
      181 gaggacaaag aaatgagtcc atattatgca caaggaaatc tgatatatgt acgaccgaaa
      241 aatacaaatg agattttagt cggagatatt attacatatt atgaaaattc aggaaaaaaa
      301 atctcgacgc gtcgcgtaat agccgttgaa aacaaccacg aagattttta tacaaaagct
      361 gatacaaaga cttatattga gcctggagta gtaaaaaaga gaaatattat cggtagtccc
      421 atctttcaga ttccatatat tggactagtc ctttcaaaaa cggcatttgc atggattcag
      481 gggttattct ttacgattgc tttttgccta acaggaatca cagcatggaa tacctttgta
      541 gagttgaaaa agaagcggag acgaacacag aaatttttgt aagaatgtta aaaacataaa
      601 tgtattccag agatccaacg actcttctga acatgaaaca cgagaaaaca ctcatgcagc
      661 ttgtagattt agtcagttgt tcgtataatg tgtgaagagg aggagtagta tgcgcaatga
      721 aatgatgcat cgtcctgtag tcaaagaaga actattggat tttatgcgta cccagcaaaa
      781 aaaattaccg ggtgagctag gcaaactgga agaagaagct catgtagcag aagtaccagt
      841 tattccccat gaaacagttg tatttttgaa atttttactt ggacagcttc agcctgaacg
      901 aatcttggag atcggagcag ctatcggttt ttcatcaagt gtcatggcca cgacaatcgg
      961 agaaaacgct catgttacga caattgatcg tttcgatgtc atgatcgaga aagctaaaaa
     1021 aaactatgaa cgtctaggac tgacagataa agtgactttg cttgaaggtc aagcagcaga
     1081 tatcttgccg gagttgtctg gtccgtatga ctttattttt atggatagtg caaaatcaaa
     1141 atatattgaa tttttacctg aatgcttacg tttgttaaga aaaggtggcg ttttgatggt
     1201 agatgacatc ttccaaggag gaacgatctt gctcccagat gaagagattc cccgtggcaa
     1261 acgagctatc catcgtaaat taaatgaatt tcttcgtgtt gtgatggatc atcccgattt
     1321 gacatcgaca atcttgccat taggtgatgg tgtgatctta atgacaaagg aatcagaaac
     1381 aatcgagttg tgatacatga ttatggcagc ctgtagataa tcgattgaat cgagggctca
     1441 attcgaactt gatgtaaaag tttctatatt tgtaagtcaa ttaaaggata caggaaatct
     1501 aaataaacag gagtagcatg aagtcaatgt agctcctgtt ttcaattgat aaccttagaa
     1561 cagctgaagg agaatgtctc gttttaaaag attgatttag tagttgggcc aaaataattc
     1621 attatttgct aattaaataa ctatttcgta ttgacagaag caatttaatt atctataatt
     1681 attgatgttc cgctgattct tgtcatagta gctcagctgg atagagcatc cgccttctaa
     1741 gcggacggtc gggggttcga atccctcctg tgacgctaaa aaaccgttag aaactgaggt
     1801 ttctaacggt ttttctttgt tttttgtgcc aatattgttt gttttcaaag gcaaataaaa
     1861 taaaaaaaca tccttatcga gtacaattat aaggagcaaa aaaataaaac tcatcagaaa
     1921 agaggaagaa gatatgacga tactatctag aattttagtg actctagttg caattgaatt
     1981 tttttatatc atgtatatcg aaacggtgag aactgattct gacacgacaa gtagggtttt
     2041 taaaatgagt aaagaagagt taagcagaaa atctgttcaa acattgttta agaaccaagg
     2101 agtgtacaat ggactattag gagtgggact actttatgga gcttatcttt cttccgcatc
     2161 taaagaaatc acaagtatgc tattaatatc tatttttttt gttgcattat atggcagttt
     2221 gactagcgat cgttggattt tactcaaaca aggtgggcta gctctgttgg cattgctttc
     2281 tattttctta taaatataga aaaaataaga atttatctaa caggaaactt tgtgaaaaat
     2341 attactttat ctcatgaaat atgatattct aatgaatggt attttagaaa atttcatgaa
     2401 aaaggagtta gataggtgtt caaaaattat gtttcctcac taaaaaaaga attttcaggt
     2461 tatgatcttt caagatttca gaaagatctt ttggctggga tcacagtagc tgctgtagca
     2521 ttgcctttag ccttggcttt tggggtatca agcggagctg atgcggccgc aggtttgatt
     2581 acggctatca ttgccggtat cgttattggc ggtttatcag gcggttttta tcaaatatca
     2641 ggtccgacag gtgcgatggc agctatcttg atgtctattg cggcaacaaa gggaatgcaa
     2701 ggtgtattgc ttgctacttt tttagcgggt ctatttttac ttgcagcagg acttttccga
     2761 ttaggaacac tgacttcttt tatcccagca cccgtaatta cggggtttac ttcaggtatt
     2821 gctatcatta ttgcattagg tcagatcgat aatttatttg gtgttcactc ggaaggtaca
     2881 aacgttattg aaaaactaag tagttatcaa acgataggat ttccgataaa cacagcctct
     2941 ttgatcatgg gagggctagt cattttaggg atgtttatct atccaaaaaa atgggcgaaa
     3001 cgaatgccta gttcgctgct ggctatcatt ttggcaacag cagttatgct attgttccat
     3061 ttaccagtag caactgtggg taagattcca caaacattga tcagtagtaa tcgtttgaat
     3121 atgggagatt ttagcttttc tgccttgcaa caagtggcag ttccagctat tagcattgct
     3181 ttattaggaa tgattgaaag cttgctatgt ggtgcttcgg ctggccgaat ggcaaataag
     3241 cccttagata gcaatcaaga attaatggcg caaggaattg gaaatctgtt gcttcctttc
     3301 tttggcggga ttcctgctac tgcggccatc gcccgtacaa gcgtggccat caaatctggt
     3361 gcacaaacta gattagctgg tatgatacat gcagttgtct tactgctctc catgcttgtt
     3421 tttgcgccag tgatgtcaca gattccaatg ccagcacttg caggtgtttt gattgtgacg
     3481 gcttggcgta tgaatgaatg ggaaacgatc aaggagctat ttactaataa atattggtca
     3541 gcagttctat tgtttatcct tacgatgatt tgtacagtta tctttgattt aagtattgct
     3601 atcgtgatcg gggtgatcgg cggctgtgta ttttttgttg tcaaaagcgc tgctatcacg
     3661 atttcattag aaacgatcga ttggcaaaga atggggctgc cagaaacgga aaagctgaat
     3721 aactggacag tagtgtatat tagtggtcca ctgtttttta tgtcttcaga aaaactcaaa
     3781 aataaattgg aagaactaaa tcatcacgat ggtgtcattt tctccatgcg aggcgtacca
     3841 agtattgatt tgacggcacg tgcagttttt gatgaatttc aagaaaaagc agagcaaaga
     3901 ggacaaacga ttatctatac ttccttgcag ccagaagtag aaaaacaact ttctcacttg
     3961 tgggaacata cattcacgca tcaacatcaa acagttgctc atgcgttgac agctttacat
     4021 gcgaaatata ctgcaattag ccattaaaac aatgaaaaag aacagacaag actgtaaact
     4081 gaagatttta tggtcttgtc tgttcttttt aatattgctg taaaagtgaa gctagtactt
     4141 gccagtcttg atggataaca ggtacaaggg agcgattgta gaaaatagct gctggatgaa
     4201 aaagtggaag aattcgatag tttttttctg accattcgta ggcatccttt tgttctgtta
     4261 attgtaagac aggccctgta tagagttgac catgattttt ggtaaccgta tattctttgc
     4321 caagtaaacg ttgaagacca atgtttccaa gtgtcgcaat taattttggc tggaccttca
     4381 tcagttcgta atccaatatt ggtgcatgcg caaatacttc ggaacgagta ggtgtccgat
     4441 ttggataaac agtttctgac tgttgtgtcc gtttattgat ccgatgagtc acgcgatacg
     4501 ggcggctcct tacagcactg gtgatataca cctcttctcg tgttaagcca gttgaagaaa
     4561 gtgcctgcat caattctttt ccagcttgac cgctaaaagg aacatgcgta tcaatctctg
     4621 ttttccctgg ggcttctccc acgagcatca gtttaggatg aagaggtcct tgcccagcga
     4681 taaatccttc tacaggacgg ccgttcattc ttaaagaagc ttcttgtatc agttcttttg
     4741 ggtattccat ttatgctcct cctttttctt ttattataag aataaaagaa acacaatgcg
     4801 actattttat ttggtagaac tgtgaggata gaaaagcaga agtattgctt gaatttttgt
     4861 tttacttttt gattttaaat ttagttgact aacggttgct gttctggtaa gctagtactg
     4921 ttgtaattgt ggactccaca gctacaaccg ctcagaacaa gtaataagca cagcatatgc
     4981 tgtcacttcg gatggcgagt ctaagtatat gagaaggagg tgctttatag catgtacgca
     5041 attattaaaa ctggtggtaa acaagtaaaa gttgaagtgg gacaagcaat ctatgttgaa
     5101 aaacttgacg ttgaagcagg agaaaaagtt gtttttgacg aagttatctt agtaggcgga
     5161 gaatctacaa aagtaggcgc tccaactgtt gcaggtgcaa ctgttgaagg tactgttgaa
     5221 aaacacggca aacaaaagaa agtcgttact ttcaaataca aacctaaaaa acattcacac
     5281 cgtaaacaag gtcatcgtca accatataca aaagtggtta tcgacgcgat caacgcttaa
     5341 tagcgtttga gaaagaaggc ctatatatga tcaaaggaac atttaagcga aatgacgcag
     5401 gtcagatcgt ttcattcact ttgaccgggc atgctgatgc tggtccttat ggaagtgata
     5461 tcgtttgtgc gggtgtttct gcgttagcga tcagcactgt caacgggatt gcctcattag
     5521 ccggttttga gccgattgtt gaaatgaatg aagaagaagg cggttatctt tataccgaag
     5581 tcacttcagg aatgacacaa gaacaaaaca atattgctga gattctatta gaaaatcttt
     5641 tattaggatt acaatcgata gaagctgaaa actcagaata tatccaaatt aaaacaatta
     5701 atgataaata ggaggtgcag accatgttgt tatctatgaa tctacaatta tttgcccaca
     5761 aaaaaggtgg cggttctaca tctaacggcc gtgactcaga atcaaaacgt ctaggtgcta
     5821 aacgtgctga tggacaaaca gttacaggtg gatcaattct ttaccgtcaa cgcggtacaa
     5881 aaatctatcc aggtgtaaac gtgggtatcg gtggagacga cactttattt gcaaaagttg
     5941 atggcgtagt acgtttcgaa cgtaaaggcc gtaacaaaaa a
//
LOCUS       DACDIG010000113         5862 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.113, whole genome shotgun sequence.
ACCESSION   DACDIG010000113 DACDIG010000000
VERSION     DACDIG010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.113"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(99..1229)
                     /locus_tag="IV247_002681"
     CDS             complement(99..1229)
                     /locus_tag="IV247_002681"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAP8778687.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1878..3080)
                     /locus_tag="IV247_002682"
     CDS             complement(1878..3080)
                     /locus_tag="IV247_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8778688.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="IV247_002683"
     CDS             complement(3140..3343)
                     /locus_tag="IV247_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP8778689.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="IV247_002684"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="IV247_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4880..5692
                     /locus_tag="IV247_002685"
     CDS             4880..5692
                     /locus_tag="IV247_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8778690.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1735 a         1209 c          938 g         1980 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa tt
//
LOCUS       DACDIG010000114         5345 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.115, whole genome shotgun sequence.
ACCESSION   DACDIG010000114 DACDIG010000000
VERSION     DACDIG010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.115"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..294)
                     /locus_tag="IV247_002686"
     CDS             complement(<1..294)
                     /locus_tag="IV247_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778691.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYIL"
     gene            complement(592..1566)
                     /gene="sgrA"
                     /locus_tag="IV247_002687"
     CDS             complement(592..1566)
                     /gene="sgrA"
                     /locus_tag="IV247_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAP8778692.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(1762..2601)
                     /locus_tag="IV247_002688"
     CDS             complement(1762..2601)
                     /locus_tag="IV247_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP8778693.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNRTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            2786..3673
                     /locus_tag="IV247_002689"
     CDS             2786..3673
                     /locus_tag="IV247_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAP8778694.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEGAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(4267..4962)
                     /locus_tag="IV247_002690"
     CDS             complement(4267..4962)
                     /locus_tag="IV247_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAP8778695.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(4946..5344)
                     /locus_tag="IV247_002691"
     CDS             complement(4946..5344)
                     /locus_tag="IV247_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP8778696.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         1706 a         1078 c          784 g         1777 t
ORIGIN      
        1 taaaatataa ccaatcgttc cctctaaaag tatagaataa actttactca gtaaaaactg
       61 agaagaaaac tcgactttta tcatcttgtt ttctgaaaga aaagataatt tatattctgg
      121 atacctttcg caaaacgatt ctgcttgttg aatcaccgaa ttcatcttag ccagggtgta
      181 gtttagatct aaagcgatat ccttcaccac atataatttc tcgtctcttt cataacaata
      241 gttcaaaaat tgatatactt gcctttcatt gatatcaata aattcttcaa acaaagtaca
      301 tactcctttt tatctattat ttagacttat tccgacagct acatgaataa cgtacaaata
      361 gattcgttac tgatttttca gagatactac catgcgcagt gtgcattgtc aattattctt
      421 gctcttattt tccatttgtg ttcctgttat ttcctgtttc tcaaatatgt tagtaaacaa
      481 aaatagatct ggcaaagcta caacttcgcc aggcaataca gcactctcga atgaaatatt
      541 cactcaaaaa tctatcgttt tattcaattt tagatctgtt gcttttattc tttatttcac
      601 tcgatggaag agagaacgaa aatagcttgc tcctacaatc aaaatgagta acacacccat
      661 ccatgtaatt agcttgttca aggttctact accagtttct ggtaaatcgc tttttctatt
      721 atctgtttct ttcgcttttt cttgatgatc aattccacga gaaacaaaat caggctgttt
      781 tagcacactt tttaggttgc tccctgcatt tctcttttgt tccttagttg gtatgatagg
      841 taccaaatca agttcattat ttacttttcc tgtgctgctt tcgctgctcg tgcttcctgt
      901 gctactctca ctactcatgc ttcctgtgct actttcacta ctcgtactct ccgtactgct
      961 ttcgctgctc gtgcttcctg tgctactttc actactcgta ctctccgtac tgctttcgct
     1021 gctcgtgttt tctgtgctac tttcactgct cgtactctct gtactgcttt caccgctcgt
     1081 gttttctgta ctgctttcac tacttgtgct atccgtactg cttggcggtc caacaactgt
     1141 ccaactgctt ggtttcaaat tctccgtgat cgtcatctgt ccatcataaa aaacaatcaa
     1201 atcaacttta tctttactct tgatctcatc aatcgatagt tctgcagaaa tcattattgt
     1261 atactcaata ccagcggtcc attgtacgtc ttttaaataa acttccccaa taactttttc
     1321 tgaacttcca ttaccatccc aaccatatgt tagaacagca tgtacatcac tagcattcac
     1381 gcttttgttt ggtttgactg gaatagtaat gacttgttgt gtcatgtcat aaacaatccg
     1441 atcagttttc acagtaaatg aaagattctc atttgcccgt tcatttgcag aagtactgat
     1501 tccaatcatt gctgcaatta tactcagccc aagaataaca aataatacgg tcgctgtttt
     1561 cttcatttct atcactctcc atataattac cttctatttg ataaaggaat tatataaaat
     1621 gaaaatgatg agaacacgac agtcagttgt gaaaatctaa caaaagatgt cgtgtattcg
     1681 cttgaatttg tttacttttt cgtttaatca atgacagact taaaaaagca agaccgatat
     1741 cagccttgct ttcttcatct tttattgata aacagcagtg tatttcttca tccgctttgt
     1801 tttatatgca tagagtaaca aaccaacaat caagactaac aagaataatg tagttgatat
     1861 agatgcttca gccccaaagt ttcccccagt aattattgta ttttgcgatt ggatcagagt
     1921 ggagaaaaaa ccttgttctt gcgttccgct gactggtata cccaaaatag ctccttgtgt
     1981 ccgattccaa gtaatatgag caccaatagc cgtaaataaa cttcctgtac tgtagataag
     2041 tgcgctgaaa aagcaacctg ctaaaaagat attgaaaact gccagaaatg tcatgttagg
     2101 attcaatccg tgcaaaagag caaataatgc agcagatcca atcattgcta ttaacggacc
     2161 aaatctaagc atctgatgct gaaaaaagcc acggaataaa agctcttctg atgcccccac
     2221 tgcaataaat aaaataaaac tcgttatcag cggcacaatg tttgcgtttc ctgaatctaa
     2281 ccgatacgcc cccgtcaaat ataatggact agtaataagc agaaacactc caattccaac
     2341 aacgagacct cccgctaaat acccccagtc ttttcctcga acagaaacac cataatcttc
     2401 tattcttttt ccttcgacct tactagcaag aaataaagct agtaaaaaaa cagctaaatg
     2461 tccgaccgta ccggcaattg ctccaatcca ttcactattt ccaataaaca tatccgcaag
     2521 tgcattattg acaagaattc ctaaaacaaa accaactaag actattaaaa cagatccaat
     2581 caaccgaata atcctgatca tctttcatcc ctcctttata ttaagtttaa caaccctttt
     2641 ctttcttgtc tcgtgaaaac tatgtaaatg ttctccatca tagagagaat agatttttac
     2701 gaactaagaa tatctacagc aaccttacga aaatgaaaga ctgtctccct aattcttctg
     2761 ttagactgtt tttaataagg aaaggatggg aaattttttg aaaaaacaac tactaattat
     2821 gtgcctttca gcatgtgcaa ttttactatt ctcaggttgt agcaagtcca atgaaacgat
     2881 tgcaacgttt aaaggtggaa cacttacagt tcaggatttt tatgataacc caaaagttaa
     2941 atcaaataat caaacactat taaaaaaaat gattgtcttc aaagcatttg atgatgtata
     3001 cggaaaagag atttcttcaa aggaagtaac aaaagaatat aatgaacaaa tcaaaaaact
     3061 cggtccaaac tacaaggaac aattaaaagc agttggccaa acagaagaga catataagaa
     3121 actctttaaa cagatgctgg ctttccaata cggtttgaaa gccaatgtaa aattaacaga
     3181 taaagatcta gacactgctt ggaaagaatt ctatccagaa gttagtacac agatcattct
     3241 attttcgact aaagaaggag cagataaagc aaaaaaagaa gcaaatgaag gagaaaattt
     3301 ctccaagctt gttcaagctt atggaaaaaa taaaacatta aaggaaactg atggaaaaat
     3361 gaattttgat tcaaccaatc cagagattcc tactgaagta aaaaaagcag catttaaact
     3421 aaaaaacggg gaagtttctg atatcattcc tgtaacagat ccaacgactt atcagcaaag
     3481 ctattatcta gtaaaaatgg taaaaaaaca ggataaaggt tctaataaag acaaatacaa
     3541 atctgagcta gaaaaaattg ctaccgaagc aaaaactctt gatacggaat tcatggataa
     3601 aacaatccgt aaagtgatga aaaaagacaa tgtaactatt aaagatcctt atgtaaaaaa
     3661 tatattcaaa taaacaaatt tgacccaatt ggggatgtgg caaaatttga gaagtgattt
     3721 ttggaaattc cttcttattt ttgaggcaaa ccacttttgc cacaacttca tcggggcctt
     3781 tttttatttg taccaaaaca aatcactgga tttgttacaa tcatgaggat ataatttaaa
     3841 aaagtttttt atttctatca catgaatcaa tgcgccatta agtagattct ttcatgcttc
     3901 cctatttttt cacttgccgt atcgcttcaa aaacaactat cataaactca aactcgactg
     3961 aaaagttatt ttaaaaaaga ctaaaaatat cccttctgga cttgttcttc ttattctgtt
     4021 atactagaga aacacgaaag atatttttat gcgacacttt tcatttgttt ttatgaaatt
     4081 tattcaaaac tggcgtaact tcagagttac aaggatgtat tagaggttag ggaatacatc
     4141 gtttaaatga gaaataagta atagttagta atagtcaccc aaactttaaa ttaatgaata
     4201 ttgaatcgtc agatagcctc ctcgagtttt cgagaaggct atcgtttatt ttatggagaa
     4261 attgattcag aagtaatcag cgaccttttt tagttcacca atcgtttctg cttggaaagt
     4321 ttgcacccaa tcttcttgga agcccctcac tgctttatca atcgagggca tctctaatac
     4381 ttgatctagt agctctttac ctaacgttat cgcatgagct ccagatcgaa tggctgtagt
     4441 gacttgttca caatttttga aactagctgc taatattttt gttggtcttt gcgtacgttc
     4501 gatctcagtc cgcaacgaat caatcacttt catagcatca atgccttgat tttgcatcct
     4561 gttataataa ggtgcaatat aatctgctcc attattaata gctaaatacc cttgtaattc
     4621 tgtgtaaata gctgtaccgg taatcaaata accttctttt ttcaactttt tgatcgcttg
     4681 aaggcctgct tgattcaccg gcaccttgat aaacgtatct tttcccagtt cttctatgat
     4741 aactcgagca tcctctatca tttcttcttc cgttcttcct attgtctgaa tatggatagg
     4801 aatctgtccg attatttttt tgatttcttt catatgcgag aagaagtttg acgctccttc
     4861 ttttttcaga attgtaggat ttgttgttac gcctgttaaa ttcaaaaact gttgacaaga
     4921 ataaatactt tttgtatttg ctgtatcaag catgatttcc atatagcttt cccccattct
     4981 gatcctcttg atcgatttta atgattgtcc cctttcttaa atgcggcatt tccttatctt
     5041 ctacatacat tgttcctgga agttccggct catgagatcc atcaaatttt atcgtgatat
     5101 gtcctaattc attcaaatta gtttggacaa catctcctac tgctgtgatt acatacattt
     5161 gtttatcaaa acagagcttc atatcacaag ttatttcttt tcttacctga ttcaattcaa
     5221 tcttataaca ataatctgtt aatgtttccg gtgcttcatc tccaaataaa atcattattt
     5281 tttctttcct gaatacttct gcttctatac caacatctgt tacaaccgtt tcaaagatcg
     5341 tcatc
//
LOCUS       DACDIG010000115         5218 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.116, whole genome shotgun sequence.
ACCESSION   DACDIG010000115 DACDIG010000000
VERSION     DACDIG010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.116"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            5..1012
                     /locus_tag="IV247_002692"
     CDS             5..1012
                     /locus_tag="IV247_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8778697.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1024..2028
                     /gene="neuB"
                     /locus_tag="IV247_002693"
     CDS             1024..2028
                     /gene="neuB"
                     /locus_tag="IV247_002693"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HAP8778698.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2025..3173
                     /gene="neuC"
                     /locus_tag="IV247_002694"
     CDS             2025..3173
                     /gene="neuC"
                     /locus_tag="IV247_002694"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAP8778699.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            3146..3823
                     /locus_tag="IV247_002695"
     CDS             3146..3823
                     /locus_tag="IV247_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP8778700.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            3813..4955
                     /locus_tag="IV247_002696"
     CDS             3813..4955
                     /locus_tag="IV247_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAP8778701.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            4968..>5218
                     /locus_tag="IV247_002697"
     CDS             4968..>5218
                     /locus_tag="IV247_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8778702.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALN"
BASE COUNT         1934 a          677 c         1025 g         1582 t
ORIGIN      
        1 ataagtgttt aaaggaaaaa caatattagt aactggcgga actggttcca ttggaagtga
       61 aattgttcgt cagcttttaa aatatgaacc aaaagctatc agaatttata gtagaggtga
      121 agaaaaacaa ttttatatgc aacaagagct ctcaaatcac actaatttga gatttttaat
      181 tggtgatatc agagactctc aaaggattga ttatgcatgt cgtgatgtgg atattatttt
      241 tcatgctgcc gcaatgaaac acgttccagc aagtgaatat aatcctatgg aagctgtaaa
      301 aacaaatgtg atgggaactc aaaatgtaat agatgcagcg atcaaaaata atgtaaaaca
      361 ttttgtaggt atcagtacag ataaagtagt gagtcctact aacaccatgg gtgcgacaaa
      421 gttattgagt gaaaaattaa taatagctgc caatcagtat aaaggaaaaa cagaaacaat
      481 attttcttgt gtaaggtttg gaaatgtcat ggggtcaagt ggatctgtta ttcctctttt
      541 tagtaaacaa ataaaaaaca ataaagatat tactttgaca gatgttgata tgacacgatt
      601 tatgatgtca ataccggatg ctgcaagatt agttctgtct gctgctatga attcaaaatt
      661 tggagaaaca tatgtcttaa aaatgccggc aatcaatatt ttaactttag ctcaatcatt
      721 aataaaaata tataatgata cacatgatga aaaatataga ggtgaaataa aagttgtagg
      781 tatcaggcca ggagaaaaat tatatgaaga gctaatgact acagacgaaa ttgaacgctc
      841 atatgaaaat gataaaatgt atatcattcc accacactat catgctactg acgaactgta
      901 caaagattat gaaaaaatta aaatcactgc atactcttca gatgcaacag aattaatgag
      961 tggacaggaa tttattagaa tcgcaaaaga gaataatgtc gtgacttggt aaggagtttt
     1021 atgatgaaat cagaagtata tgtaattgca gaaattggtg taaaccataa tggaagtcta
     1081 gatttagctt tagaaagtat tgataaagct aaagaatgtg gagctaacgc ggttaaattt
     1141 caaacattta aaacgaataa gctagtttcc aaaaaggctc caatggcaaa atatcagaag
     1201 gataatctta aagaggacag ttctcaattt gaaatgctaa aaaaattaga attatctcaa
     1261 tctgatttta ttagaataaa agattattgt atacaaaata aaattgattt tttgtctact
     1321 ccttttgatg aagaaagtgc agactttcta aaacaaattg gtgtacaagg atttaaaatt
     1381 ggctcaggag atttaacaaa tttacctttt cttagaaaaa tagatatgta tgggttgcca
     1441 gttttattat ctacaggtat ggctgatatt catgaggtca gtgaggctgt agattgtttc
     1501 atagatagtc cagttactat tttacattgt acttcaaatt atccggctag ttctgaagat
     1561 attaatttat tggcgattaa cacattaaaa gaaatgttcg ggatacccgt tgggtactcc
     1621 gatcattcat tgggatatga tgttggtatt tgtgctgttg cgatgggtgc taaggtaatt
     1681 gagaaacatt ttaccttgga taaggaacta cctggtccag atcataaagc ttctttagat
     1741 cctcaggaat ttgcagattt tgtaaagcat attagaaaca ctgaaatatt tttaggtgat
     1801 ggtgttaagc gagcaatgcc ttctgaactt agtacaaaag aagtatcaag aaaaagccta
     1861 gtggtcactg agaatttaaa tgtgggagat attcttacag agaaatctct tacaataaaa
     1921 cgtcctggaa atggaatagc tcctaagttt ttatatcaat atattggcaa aaaagttaaa
     1981 agaccaataa gtgcagatag tgttctgctt gaggaagatg ttgaatgaaa aaaatactta
     2041 tagtaagtgg aacacgagct gattatggaa tatataaacc tgttgctgag aatttactac
     2101 aagataattg ggatgtatca tttgttgttt ctggaatgca tttatcccat aaatatggat
     2161 ttactaaaga attgatctta gctgacggat ttagaattag tggagaagtt caaagtttgt
     2221 ttttagagaa caataaggga aacatggctc ggtctgtggc attagagata aatggtttca
     2281 caaatatatt tgaacaaact agccctgatt ttgtattatt actaggggat agaggagaaa
     2341 tgttggcagc tgcgatagct tgtttgtatc ttggaatcaa aacagcacat attcacggag
     2401 gcgaagtgtc tggtactgta gatgaatcaa taagacatgc cataacgaaa atagcatctg
     2461 ttcattttgc tgctacggaa aaaagcaaag agcgtttaat aaaaatgggg gaagattctt
     2521 ggagagtttt tcccgtcgga gctcctagaa tagatactat tttaaataat agattaccta
     2581 gttttagaga tataaaagaa aaatatcatc taaaatttga aaagaaaaaa tacgttttgc
     2641 aagtatttca tccagttgta accgaactag aaaatataga aaaacaaatt ttagctttga
     2701 tcaattcaat ggaacaacaa tctcaaccaa ttatttgcat tttacctaat agtgatgctg
     2761 gtagcgaaat tatcagaaaa atgtaccaga aatatttaaa aacggatatt atttatatta
     2821 cgaatttaga gccggaggaa tacctgacta tattaaaaga gtgcaaattt atgatcggaa
     2881 attcttcttc tgggataatt gaagctgcat cattcaaaat accagtaata aatttaggaa
     2941 ccagacaaaa tggacgagaa caatctaaaa atattataaa tgcaaatcta aatgaaaatg
     3001 aaattgaaac tgctttgaat aaaatacaag atcctcagtt tataaaaagc ttgaaaaatg
     3061 ttaagaacat ctacggtgac ggcaacagtt ctagaagaat aacaaaaaca ttgaatcaaa
     3121 tattgaatga tggggaagat attaagtgga ttcaaaaaca aatagcttat taaaatatac
     3181 tagtgatcca tacattatta ttggggctgg cggacacgca aaagtcatta ttgaaacctt
     3241 aagattagaa aataagttaa tatatggatt ttttgatgat aagcaaaata aaatgcaaga
     3301 tctgaaatat cttgggaaaa taaacgaatt gaatagctgg gcggatagca aaaaaaaaat
     3361 ctataaattt attgtagcta ttggtgactg tgtagtaaga aaaaagatta taaaaaaatt
     3421 gaatatgcca agtgagcaat atggaatagc tgttcatcca agtgctatag tggcaaaaga
     3481 aatttcaatt ggttatggaa ctgttatttt tgcgaatgct gtaattaacc cagatgctgt
     3541 aattggagag catgctataa taaacacagg tagcattgtt gagcatgatt gtagaattaa
     3601 taactatgtt catttatctc caggtgtatg cttagcgggt ggagtacatg taggtgtagg
     3661 aactcaaata ggaattggtt ctcaatgtat ccagatgaag aaaattggat cttggtgtat
     3721 gataggcgca gggagcacta ttgttaagga tattccctca cattccttag cttatggcaa
     3781 tccagctaaa attaagaaag aaggaattaa ttttgaattc tagaatttta ttatcaagtc
     3841 ctcatatggg aggaacagaa gaaaaatatg ttgctaaggc ttttaaagat aattggatag
     3901 cgccattagg tgaaaatgtg gataaatttg aagaaagtat tacaaaatat actggttcta
     3961 agggcggagc ttctgcgtta agttcaggta cagctgccat acatcttgcc ttaagactgc
     4021 ttaatgttgg aaaaaacgat attgtctttg ctccttcatt tacttttata gctacagtaa
     4081 atcctatttt atatcaaggg gctgtaccag tgttcattga ctcagaacct aatacatgga
     4141 atatgagccc tgttgcacta gagagcgcat tagaagaagc tgaaagaaac aaccaattac
     4201 ctaaggcaat catcgtagtg aatttatatg gtcaaatggc tgattatgaa cgaattttag
     4261 ctattgcaaa ccaatttaat gttccagtga tagaagatgc agcggaatcg ttaggttcta
     4321 cttataaggg aatttcgtca ggcttatttg ggacaattgg gatctattca tttaacggta
     4381 ataagatcat cacaacctca ggtggaggag ctctagtgtc tagtgataaa aaatatgttg
     4441 agcattcaag atttttagca acacaagcaa gagatcaagc accgtattat caacatagcg
     4501 aaataggtta taattaccgt ctaagcaata tttctgccgg tatcggacgt ggacaaatgg
     4561 aagtattgaa tgatagagta gctaaacgaa gagaaatttt caatcgatat aaggaagctt
     4621 tttctggaat tgatagaatt gaaatgatgc cagaagtgga tggcatggtg ccaaatcgtt
     4681 ggttgtctac tttaacgatc aatgattata attctattga tcaagtagtt aacgtaataa
     4741 ataaactaaa tgaaaaaaac attgaaacaa gagctttgtg gaaaccacta catttgcaac
     4801 cagtatttca aggggtaaaa atgtattacc atgataataa tcttagtgtg tcagaaaagt
     4861 tatttaatag cggcttatgc ttaccttctg gaagtaatat gacagatagt gaacaagaat
     4921 ttgttattga tgagtttaaa aaaatattca agtaggtgtg atattttatg aacataggaa
     4981 ttattggttt aggttccatg gggaaaagaa gacttagact cattcaagaa cactttaatg
     5041 atattttact atgtggtgtt gatactaatg aggcgagacg aaaggaagtt gaaaaggaat
     5101 ttggaatacc aacttataat agtctaacag aggcaaaaaa tttgtttgaa ataaattcag
     5161 tttttgtttg tacatcccct ataacccatg aatttattat aaaagaggct ttaaattt
//
LOCUS       DACDIG010000116         5213 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.117, whole genome shotgun sequence.
ACCESSION   DACDIG010000116 DACDIG010000000
VERSION     DACDIG010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.117"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            138..2072
                     /locus_tag="IV247_002698"
     CDS             138..2072
                     /locus_tag="IV247_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8778703.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            2529..2945
                     /locus_tag="IV247_002699"
     CDS             2529..2945
                     /locus_tag="IV247_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAP8778704.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            2959..3525
                     /locus_tag="IV247_002700"
     CDS             2959..3525
                     /locus_tag="IV247_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAP8778705.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            3548..4534
                     /locus_tag="IV247_002701"
     CDS             3548..4534
                     /locus_tag="IV247_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP8778706.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(4689..>5213)
                     /gene="scm"
                     /locus_tag="IV247_002702"
     CDS             complement(4689..>5213)
                     /gene="scm"
                     /locus_tag="IV247_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP8778707.1"
                     /translation="TSASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSD
                     STDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSD
                     TRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVI
                     GLRITFMSYKKSKR"
BASE COUNT         1588 a          955 c         1148 g         1522 t
ORIGIN      
        1 atttgaaaaa aatccaaaag gagttagaca aaagaacaga acatctatat aatatatgga
       61 gattgtgtaa taagtatcaa actaaaagaa ttatcatata ttcattatat agatagtcta
      121 taaggagcgg atgatcaatg gcaaagcaaa atattgtcgt tgtaggagca ggatatgccg
      181 gtgtttcagc tacaaaatac ttggcaaaga aactgaaaaa agaagatgta gcaattactc
      241 tgatcgatcg tcattcgtac catactatga tgaccgagtt gcatgaagta gctggcggac
      301 gtgtagaacc agaagcaatc caatacgatc tgcagcattt gtttgctcgt caaaagaatg
      361 tacaacttgt cacagacact gtaatcggta tcgataaaga acgaaaaatc gtcaagacca
      421 aattaggttc ctatcctttt gatcagctga tccttggaat gggcggagag ccgaatgact
      481 ttggtacacc tggtgtcaaa gcacacggat tcaccctttg gtcatttgaa gattctgtaa
      541 ggattcggga acatattgag aagacagtag caaaagctgc attggaacca gatgatgaac
      601 tgcgtaaagc catgctgact ttcgttgtct gtggttctgg atttaccggt attgaaatga
      661 ttggggaact gatcgactgg aaaaatcgcc tagccaaaga ttataaatta gatccaaacg
      721 aaatcacact gatggtcgta gaggcaatgc cgacgatctt gaatatgttg gatcgaaatg
      781 atgctgcaaa agctgaacgc tatttgcgta agcacaacgt ggaattatta ttgaatgcac
      841 caatcgtaga agtggctgct gatcatatca aattgaaaga cggacgcaac attccgactc
      901 acacactgat ttggacagct ggtgtcaaag gaacttctga cgctgctgac tttggattag
      961 aatcagcacg cggacagcgg ttgatcgcca atgaatatat gcaggcaaaa ggctacgaag
     1021 ataaaaacat ctatatcatt ggtgatttag tcttttatga agaattccca ggaacaccga
     1081 caccgcagat cgttcaagct gctgaacaaa caggacacac agccgcagcg aatatcgtgg
     1141 cttcgatcaa aggcggagaa aagcataaat ttaaaggaaa ctatcaagga tttatggttt
     1201 ctattggtgc caaatggggg gtagctaacc tatttgataa gatccattta agcggatttt
     1261 tagctatcgt aatgaaacat atcgtgaatt tgaaatactt ctttgatatt cgttctggtt
     1321 attatatgtt ccaatattta atgcatgagt ttttccatat caaagatgat cgcaacgtga
     1381 ctcgaggaca ttcttctcgt tacggcaatg tcttatggag tgtgccactt cgtattttct
     1441 atggtttagt ttggttcgtt gaagcttcca aaaaaatcat tgggaatggt gaagtgttca
     1501 aaccaagtac ttggtttggt gaaggatcat ggttcactgg aaacagtgtg ttcccttgga
     1561 catgggaata caatgtaaca gatgttgcca gtggtgcttc tgctgctggt gatgcaacca
     1621 cagctgccag cggagcaggg gcaagtgccg gagaagcagc gactcaagct gcacattttg
     1681 gtttgagtta tgcatacggt gaagaaccaa tggctgtgat tgagaagtca ccagactggt
     1741 tcacaaagat catgcaagtg atcatgccaa acaatgatgt agcaatgttc ttccaaaaat
     1801 tcatggtatt ctttgaaatt gccttggcat tagcattgat tgctggatta ttcacatggc
     1861 tatgcagtgc gacaacgatt gtattggtga tcacgttctg cttatcaggc atgttttact
     1921 gggtaaacat ttggttcatt tttgttgctt tcgcattgat gaacggttcc ggacgtgcga
     1981 ttgggttgga tcgctgggta attccatggc tacaacgtac actaggtaat tggtggtacg
     2041 gtagaccaag ttcccagtac ggcaagaaat aaatagataa agaacgttat ttgaatgcaa
     2101 gatttcaata gaaaggggct gagacaaacc gtcacagcct cctttttatg taaaattaaa
     2161 ggaataagat gcccgagata agcctccatg agcaagacac aggtgtcaca acctctgtta
     2221 caacctcttg ttataaacgg tgttccagaa gtagcttctt cgaaaaataa gccgaaattt
     2281 cacaaaaact tgaaaagtaa ttttcgtaaa ttccttctta tttcttgaag ttgcccactt
     2341 ctgtcacaac ctcttgttat aaacggtgtt ccacctgctg cttctcggta ataagcccaa
     2401 agaattagaa aacatggaaa gctgttttct attcttcgtt cttattactc aaagcaagac
     2461 acaggtgtca caacctcttg tttttatgct aaaattgggt gagttaatct ggaaaaagga
     2521 gaacaaaaat gagccttaaa gaatttgctg aaaaaagtct gctcaaacct tgggatgttt
     2581 ttattatttt gcttttgact gttctttctt ttttaccagt catgattttt ggtcttcagc
     2641 aaaacaccgc aacagcagaa aaagaagcgg tactgcgtgt ggatggcaca gaaatcaaaa
     2701 cattcccttt gatggctgga gaagagagca acacatatac ttacaaagat gaacacggag
     2761 attataactt gatcgagata gatggcgatc gagtacggat aaaagaagca gattgcgatg
     2821 accagatctg cgtacgtcga ggctgggcaa cgaaaaatgg cgaaaccatc gtctgtttgc
     2881 cacataaatt agtgatcgaa gtccgttcaa cagatggagg tgacgaagac agtctgattt
     2941 attagacctt cacacaccat gagtaaatta caaaaaatga tttatatttc gctacttgtt
     3001 gcacaaggag tgatcattgg tttattggaa aatatgatcc cttatccttt ttcttttgcg
     3061 cctggtgcaa aactaggctt agccaatcta atcacgatcg ttgccatttt taccatgaaa
     3121 aaacgagaca gcttcttact actttggcta cgactattcc tgactacttt gctaggcgga
     3181 acgatttcca cattccttta tagtatgagt ggtgctttgc ttagttattt tggaatgtta
     3241 ttggtcaagc aattaggacc aaaacgtgtc agtatcatcg gaattagtgc ggccggcggt
     3301 ttcatgcata atgtcggaca gcttttgatg gcttctttca ttgcacaatc atggacagtc
     3361 atgttgtact tgccagtcct ttcagtttta gggattttat cagggattgc aatcgggatc
     3421 gcagcgaatt atcttttacg acatgtgcag acacttcaac gattccaaaa agattatgag
     3481 cagggaacca aacaaaaatg gagcccaatg tttaccaaaa aataatcgtc acacaaggag
     3541 tttttttatg aagctacatc caatgtggga aaactatcct gctttaagta aagaattgac
     3601 agaaacacta aaacggatgg aagaaactgt ccgcctgaaa aacaaacctg tcgaacaagc
     3661 aatcaaagaa acgatccatg ccggcggaaa gctgttgcgt cctgcctatc agctattgtt
     3721 ctcacaattt ggaccagagc aagatcgaga caaagccatc gctttggcag cttctatcga
     3781 aatgctccat acggccacac tgatccatga tgatatcgtt gatgaagcgg atctgcgacg
     3841 tggtcagcct aatatccgta gtcagtttgg taatgtcgtt tctgtctatg caggagacta
     3901 tttatttgtc tgctgtttta aactattgtc tgattactcc acttccttga aaagtctcca
     3961 attgaattct cgcagtatgg aaaaagtatt gaatggcgaa ttagggcaga tggatgatcg
     4021 ctataattat gaactttctg taaaagaata tttggaaaat atttcaggta agacagctga
     4081 actgttccaa ttaagttgtt ctgttggcgc ttttgaaagt ggaacaagcg agcgcttcgc
     4141 aaaaaaagca ggagatatcg gactttctat cgggatggcc tttcagatta tcgatgatat
     4201 tttagattat acaaaagaga gccaggaaat cggcaaacct gtactagaag acatgcgtca
     4261 aggggtctat tctttaccgt tgatctactc tttgcaaaaa aacaaaccaa agctactacc
     4321 ttatttagag aagaaagcgg caatgacaga agaagatgtc gacgccgtac gcaaaatcgt
     4381 tgaacataca ggaggcgtgg aagaagctag gaagttagcg gcctcttaca cagaaaaggc
     4441 tttgaaggaa ataaagaaat tacctgccac ctctcttcga acaaaagaga atctcttttc
     4501 ccttacccaa ctgattttgg atcgcaaaga ctgaataggg aaaggttcga tacgtaaata
     4561 aatttctcag aaggaaatct acgtgttttt tgacaaattg aattgtagtt cttgtattta
     4621 tgataaaaag caaaaataaa gagcaacgtc cgttagcgac gttgctcttt tttatcttga
     4681 atattttttt accttttcga tttcttataa ctcataaaag tgattcttaa accgattaca
     4741 agtaatataa cgcctgcgag tgttatccaa ttattcgatt gacttccagt tttaggaagt
     4801 gctctttttg atttgtttcc attgtcattg cttgttttat tagttaagtt aaagttcgtc
     4861 ctgttatccg aacttgtttt tgagaaggag acacttgcat gacgtgtatc gcttgtggta
     4921 gacgacgtgt ctgtactatc gcttgaagtt gatgaatctg agctatcact tgtggtagag
     4981 gatgtatctg tactgtcact tgaagctgac gtgtccgtgc tatcacttgt agtggtagat
     5041 gaatctgagc tatcacttgt ggtagaggta gtatctgtac tgtcacttga agctgacgtg
     5101 tccgtgctat cacttgttgt ggtagatgta tccgtactgt cgcttgaagc tgacgtgtcc
     5161 gtgctatcac ttgtggtaga ggtagtatct gtactgtcac ttgaagctga tgt
//
LOCUS       DACDIG010000117         5192 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.118, whole genome shotgun sequence.
ACCESSION   DACDIG010000117 DACDIG010000000
VERSION     DACDIG010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.118"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(44..502)
                     /locus_tag="IV247_002703"
     CDS             complement(44..502)
                     /locus_tag="IV247_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8778708.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKLTRV"
     gene            complement(809..1906)
                     /locus_tag="IV247_002704"
     CDS             complement(809..1906)
                     /locus_tag="IV247_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP8778709.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            complement(2014..4041)
                     /locus_tag="IV247_002705"
     CDS             complement(2014..4041)
                     /locus_tag="IV247_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAP8778710.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            complement(4186..4917)
                     /locus_tag="IV247_002706"
     CDS             complement(4186..4917)
                     /locus_tag="IV247_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP8778711.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
BASE COUNT         1469 a         1049 c          818 g         1856 t
ORIGIN      
        1 gacttccttt tttgtatgga ttttttcatt tacacaaaat attttatact ctcgtcaact
       61 ttctcttttc aaaaaaaatt tgatattttt ttagatttct ttttagtctt tgtaatactt
      121 tataagccac agaataactc acgtgatgct gtttagcaaa attttctact gagatatatt
      181 tttcctgaaa gaaagccaat accattttca tagataaaga atccgttata taacgagata
      241 acagagcttt gctagaacat tttccattaa tttccagaca tatatccaat tcactgacgg
      301 ttactcgcaa atttttctct agatcgaact tttgtaaatc atgtttcagc ttatcgatcg
      361 tttttttcaa gagaaatgtc gatagcgaca actcttcttg aacattcgat attgactctg
      421 ataaatttga gcttgcttct aaatatttaa gtaatgaata acttctgtga tcttctttgc
      481 ttaaaaaaaa ctcataattc attagtcatc agctctttct acgtatatta ctttccatgc
      541 attcaccaac tagctgggga taaaactgat ttcctggatc cgagtcactg ttgtcagcga
      601 ttcttgtttg tataatatac atcgtcctgt ctttttttta acattttgga gagcgtttta
      661 tttggccttt ctcgcgatca aacctgttat tagaattata tcatttacta gtatgcattt
      721 ttattaataa aatggtatat tttatattgt tataaaaaga gcccaaccat cctacaataa
      781 taaaaattta ctatttgtta tcttaacctt acttttcttt ctcttctttt tttctcaaaa
      841 caaacaaaag tatgcataat aaaataaaca ataagaaaag tgaagctact aagatataaa
      901 tccaattatt gtctttttta atcgtaacat ccgtcgtgtt caattccttg gctacttttc
      961 cgtctattgt aaaatctttt tcaaataccc attgattggt atagttgatc gtagtatttc
     1021 ctttttttct cgaaaatttt ccattttcat tttcatttcc taaaacagtc attgtaaggt
     1081 gatattcacc tggggttagc ggttgaccat ttaaagcagt tggaaaagaa aaatttgtat
     1141 tcggcgcaat ctgcaatcct tctttttcct cttgatataa aacttcagaa tgtccttttt
     1201 ttgtaatttt tgtttcaata actacttgat tgatataggt tttttgatca ttttgaacgt
     1261 ttgctaagat gacatttctt gcattgattt gatctggttt aacttcatga agagtaaggt
     1321 tcggagcaac ttcgttcagt gtctgttgca tcaacaaagc aataatatag gtgtattcat
     1381 tctcaatcga taagctttga tccttagttt ctgtttggtc cttttccgtc tggatttctt
     1441 ggaatgtgat cccaccagcc aatacaccat caaatcgctc attcggcatc ctgacatgaa
     1501 aagcaacggt ttggacactt ttaggtttta gcgtcactat ctgtggatag ctcacataat
     1561 ctaccagatc aaagatcagg ctcatgtctt tttcaatctc gttttttccg tattcaacca
     1621 tcacatttga attggtggtt gcgctattca cagaaatatc gattttcact tctttttctg
     1681 tatcatttct caattccgct tttacttcta cttcctgatc cggatcaagt tgaagatcga
     1741 agtaagtttt tctcttgtcg atttgattct ctgaagttat cggcgtcact gaaaaattaa
     1801 atgcactagc tgacactgat atattgttaa aaagtgcccc taaaataact aggagtgtta
     1861 taaataattg ttgttttggt ctcaagaaaa tcgctccttt aatcatatgt aagagaagac
     1921 tataacaaaa tcttgtctct gtgggacaca agagcctatg gtacctgtgc ccttcttgaa
     1981 actcaataat tttgttatat tcttctcttc ctgttaattt cctgggacat ctgcaagtat
     2041 ccaagatagt ttcccctcaa aggttccgat tctttggttt tctacaggaa tatttattct
     2101 gattccacga tgagctgtag aatctaaaag agaggacaaa tcgctttcac ccttttgatc
     2161 actttcgaaa atcgtaacag cggatgtccc tataaccgtt gtattttcag aggtaacaaa
     2221 ggaaagagct tctggaagct caaaatctcc atttgtaaga actttctctt gtttcaattg
     2281 taatttccaa cttccaaaag cccgttcatc tataattttc aacccacctg aaaactgtcc
     2341 gaattcactt aatggtctac tggatatttt atgttttcca aaattgacat attgaggagc
     2401 agttgagaga taaagtttgc cttttctgta ataaaaatag atatcctctg tattttcgga
     2461 gaagatgcct tctgtattcg ctgaatccga gtcaaaaaca tacccttcaa tatcaatagg
     2521 agtttcttta tacttctctc ctattttccc agtgattgtt ttcatttcag caatatcaat
     2581 ctgactgtct ttatctttat aataaacatg gatatctatc tccttcagct ttgcgatctt
     2641 taggtttatt tcattcgatt gctcaatcaa attcccgttt tcaaggtatt caaaatgcat
     2701 agtgagtact ttttcaggaa ttacttcctg caaagttgtc tccaattcga ttattaacgt
     2761 atctccagca ataaaataat ctgtcagatt tagcgtgact cgatttccgg ttatcaagat
     2821 atctttggca tcaatttgat ttccattgat caagatatgc tcgggaatag ttaattcttg
     2881 tgggaggtcg atactagtct gcattttctt atattcaaga aaatccccct cgaccttcaa
     2941 tgtatttctg atagttatct tatcgtttcc tccagcttca gcgatggccc cataatcact
     3001 atcgttgact tttacatcag ctgttgatgt gacattcgat ggaagtcgcg tgacagcgac
     3061 agcattttct tgaaaaaaag tacctgttga agaagtaaac ccccaattta cttctgtaga
     3121 accaaactgc ctaatgagat caggtataat ccgagaagag cgaccgacac cttccaaatc
     3181 ataagtaaga cttctttgct ccgcgtccca tttaattatt aacaagcgcc atgtgccatt
     3241 agaaagaaat gtaggagcta ccgtcacgcc agtatgctct ccagaataat tattattatt
     3301 tggtttaggc gtgacaaaag cgacatgtcc atgccctcta ttatcagcag ttatctctcg
     3361 atccattcga tcagaagaac cactattttt atacgtatca aactcaatcg agagcgcatt
     3421 cgcaatgtaa ggttggccag aacgagtaga atatgagcca attcccatac ctggcgatcc
     3481 aataacttga ctgggattac tagccattct aggatcgcca gtcaatgtaa aggtcatgcc
     3541 atctcccgct ctatctcgtt gatttcctaa gtataaataa gatttaaatg taaaatccga
     3601 gagtaaacta atcttcttat ttgaccaaat agctcctctt tgagaccttt ctgctgggtt
     3661 caactgcagt aacttcccat tttccaatat ggacgtgcta gtaccgatag gtgtttgaaa
     3721 aattttatcg atagaaataa tattgggatc gtcaggcagc tcaaccgcag aaattggtgc
     3781 atcttctagt ccttgtttgt ccgaaccctc agcttctacg ttgtcagtgg tctctccact
     3841 actcgaaata cttaaatcct cactgctttc ttgtgtctgg ttggattcta ttaaactgtc
     3901 gaatgtcgaa gtgagtttgc tttcttcgtc tcttctattt ccatgctcca actgattcaa
     3961 ttccaaagag taccctatga tcggaaagat attagtcact aagagactgc ttaaaatgag
     4021 cacgatcctc ctctttttca atatatcttt tcctcctgat cgttaaattc ctagatgaac
     4081 tattcgtatc caaacaaatt tagcctagcc tttgacaatt tttgctcgat tacctcgcaa
     4141 gacaatatga ctacgtattt atttttaaca gttcttttca gtaaactagt tgttaggtgt
     4201 atcttctaat gtccaaacta atttagtgga atattgttgc gctaacttga cagatttacc
     4261 gggtaccgaa agctgtaccg catcttgatt ttcattttca tttgcaccaa atcgatagac
     4321 ccaagtcccc acacctgttc ctgatttagc attaattgct aacgtattgt ttcctcccgg
     4381 tgtcaacgtt aattcagaat tcaccgtttg cggcgtatat gttggatcaa ccaaagaaac
     4441 agcctgccct ttcgtaaatt tcagctgtgc tccaactagt tcatccccac tggcagtttt
     4501 aaactgttct ggttgagaga cacttaattt ccacccagat aaggtacttc tcctatctgt
     4561 cacttggaca tatgtcggtt ttctagaccc atcgttcatc acatcagcag ctgcataata
     4621 cgttttatcc gcagtagaaa ttttttgagt cccaaatttg aattttgatc cataatcaat
     4681 cgataatgcc cctcctgttc cagggcttgg aggatctgat ggatcgacag gtttattggg
     4741 atcaggattc tctggatcaa caggaggagt tacaccctca tctcctgctt caaatgtaat
     4801 cgttccttct gtgttatatt cctttgggta tacttcagtt gcagaaacaa aagtcgtact
     4861 tgctaccatg ctgaataaca tcattgctgc tgttaatacg atttcttttt tcatcattga
     4921 cattcctctt tctttattta ctaaacatat cagtcgattg gattaatcca tagcaaaatt
     4981 ctgttttacg atacttatga aataaaagaa catagaccta tatttggtcc tatgtatcga
     5041 ttagttataa atgattatca gcccgatttg atttctagtt tttattgcaa cttgatgcaa
     5101 acacaatcta gaaatacagc tgtcatagcc tttaaaataa ttcgtaagtc cccctcctat
     5161 ctatcaaaat gtgacttgac gagagtgtaa aa
//
LOCUS       DACDIG010000118         5047 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.119, whole genome shotgun sequence.
ACCESSION   DACDIG010000118 DACDIG010000000
VERSION     DACDIG010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5047
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.119"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..648)
                     /locus_tag="IV247_002707"
     CDS             complement(<1..648)
                     /locus_tag="IV247_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
                     /protein_id="HAP8778712.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWVQEYSKVLYYLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVK
                     YLNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVST"
     gene            complement(648..1018)
                     /locus_tag="IV247_002708"
                     /pseudo
     CDS             complement(648..1018)
                     /locus_tag="IV247_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1278..1826)
                     /locus_tag="IV247_002709"
     CDS             complement(1278..1826)
                     /locus_tag="IV247_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778713.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1827..2684)
                     /locus_tag="IV247_002710"
     CDS             complement(1827..2684)
                     /locus_tag="IV247_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP8778714.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2968..3255)
                     /locus_tag="IV247_002711"
     CDS             complement(2968..3255)
                     /locus_tag="IV247_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP8778715.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            complement(3245..3574)
                     /locus_tag="IV247_002712"
     CDS             complement(3245..3574)
                     /locus_tag="IV247_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP8778716.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(3651..3923)
                     /locus_tag="IV247_002713"
     CDS             complement(3651..3923)
                     /locus_tag="IV247_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP8778717.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(3923..4186)
                     /locus_tag="IV247_002714"
     CDS             complement(3923..4186)
                     /locus_tag="IV247_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP8778718.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            4420..5047
                     /locus_tag="IV247_002715"
                     /pseudo
     CDS             4420..5047
                     /locus_tag="IV247_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
BASE COUNT         1511 a         1004 c          711 g         1821 t
ORIGIN      
        1 agtagacacc gaaaagccga agagcgttcc atttcttcgg ttctttttat atattcctcg
       61 aatggtctcc atgcccttaa tcgtggaaga ggctgtacgg agactttgat aaaatttatt
      121 tcgtcgttta ataggtcgat ggtcttgttc tattaaattg ttaagatact tcacagttcg
      181 gtgctctgtc ttagtatata aacccacact ctgtaacttt ctaaaggcgg agccaagaga
      241 aggtgcttta tcggtcacaa ttgctttcgg ctcaccaaac tgtttatgga gtcgttttaa
      301 gaaagcatag gctgcttgcg tatcccgttt ctttcgtaac cagatatcta aggttaagcc
      361 gtccgcatca attgcacgat aaagataatg ccaacgtccc ttaattttga tataggtttc
      421 gtccattttc catgaataga aggattgtct atttttcttc ttccaaagat aatagaggac
      481 tttgctgtac tcttgtaccc aacgataaat cgtagtatga caaacattta ttccacgatc
      541 atataacaat tcctgaactt cacgatagct tagattgtaa cgcaggtagt aaccaacagc
      601 gacaataatg acgtcttttt tgaattgttt gcctttaaaa tgattcatta ctctgtcctc
      661 tctgtctttt ttctcaattt tacactaaaa tagatttttt ggaaaacttt gcaacagaac
      721 ccttgatctc gcttattaca atcgctttca tttgtcctaa gagtcaaatc tttttccacc
      781 ataactttta cacccaataa attttgcggt ttatttttct ctgttgaaat tctttgtgta
      841 gcctttaaac tgacaattag ctcattgggt acatacttat ttgagagtca tctaattggc
      901 tcataatcct ttctttttgg ttagtatctg cagttacttg tttagtatga gccatgaacc
      961 caaacatact caccccaaca accaatccta aatatatttt ctttttaaac tttttcacct
     1021 acatctcctc ctaatagttt tattttagag ttagctctat tacttattta ctaactcaac
     1081 aacagattat catacacttg ttaatgaaat cacaatatct cattaaaaca caccctaaaa
     1141 tcataaccgt tcctatgctc attttttgga ttttttaatc acgtaaccag aaaaataaat
     1201 cccctctatt tcatgcaaat caaagaagaa ctaagtaact accgtttaaa ggtagttact
     1261 tagttcttct ttgattatta tctaacttta ttattcattc cccacccttt gcgcagtgag
     1321 tcttcggtat cttgttttat ctgactggta gttcttatta tttctccacg atcatctaat
     1381 tctggaacaa ttggatattt ttctctgaac aagtctagtt ctttcaccac gtcattaaac
     1441 aaatcttgct tatcttcttg cgtaagattc tgtttaatat actcatctaa agcttttcta
     1501 agcaattcat ctcttgtcat attagtctca tacttttcta ctaaaactct ggaagtcgca
     1561 tttaacaaag tcgacagttt taaaggatgc ttggtggaat aaaccttatc aaactttctg
     1621 attggtgcgc caactttttt tgttcttcct ctactctgtc tttcaacatt ggaggaagtt
     1681 tttatttctt gaacttggtg cacttctaat tgcttatttt cagatggtgt aattgcttgc
     1741 tgctttttct ttgcttctgc ttcttttttt attttggcga gtcctttgtt gttcccaaaa
     1801 atatcaagtc ctttgatgtc agccaattac tccacctccg cataccaatt ctcgtgttct
     1861 tccaattctt tcaaaatact gataaataat tcgtggcttt tgcgatccca ttgttttaag
     1921 agacctttct ttgtcttctc catcgtaata cctgatccgt cgtatcgttt taagcgcgca
     1981 tttttgagta caacattttt aatcaaatgt tccccataaa tctcttgggc ttgtttatac
     2041 atttcctgat ctactgcgtc accgctttcc aacatgaaag gaataacacc agcaacatat
     2101 aaatttgtat caaattcttt cacaaaaaag tccatatact caagatattg catcgcattt
     2161 ctcatcgatt ttacttgggt ctgtaagacg acgattgccc aatccgcaat gtacatcgcg
     2221 gatttactat atacagaaat agaaggtggg acatctaaat agatgacgtc aaactcatca
     2281 aataatggtt ctagcttttc tttcatatat ttgaattggg ataatgaatc attatattgg
     2341 tctttgatcc aatcatcata catttcaaag gacatgtctg ctggaagtat atagaggtta
     2401 ttgattactt tatagacagc ctctttaggt ggaagattgt ttatcaacac gtcagtcaca
     2461 gtattttcgt aaacaatcgg ttcaccttct ccgcctcctt cttcgttagt gaagttcata
     2521 tgcgttaagt tcatcacatc agaagtattt ccttgcatat ccaagtcaat tatcaaggtt
     2581 ctttttcctt cattaactgc tgattcaaac cctaacattt gcgcgcacgt acttttacct
     2641 acgccacctt taaaattacc gattacatat ttcttcgcta ccacgttcct acctcttttc
     2701 ttttagcaat attcatttga tactattata acatatttat ttttagttac taccaaaaaa
     2761 agatatatat gtactacctt ataattaata agtaaaaggt agtacataaa taaggaaaag
     2821 ctgaaatata ttgatttata gctttcctca aagtaatgac gttatttaat ttatcgagaa
     2881 gcagagaata ctcgcgactt tagtcatgta gacgaatcag attttcgata aaaagaagcc
     2941 gtccttctta agaacgacaa caaatcacta gattaatttt ttcaactgtt cataaaagtt
     3001 ttcatgcgtt ccacataaca atactggaat aaccttgttt tctataatct cgtaagctac
     3061 tctatatgtt gtgcccgcat atttgaaccc actagaccaa atacctgata aatccccagt
     3121 tttttgttca cccttatatg gatcaatcgc aatttcatca taaattaaat tcaaaaattt
     3181 ttctttcaat ggcttctctt ttagcttttt taaatactta atgacatgtt ttgaaggaac
     3241 gatttcataa gccaatttct gttgccaact cctctttagt cattacttta gaattagtaa
     3301 gtgtatcttc agcaatatct ctaaaggaag aatgcatttt gttttttcta ttcttaaatt
     3361 ctacaagaat ctcatcttta tcgtagcctt ctgcaattat atcagacaaa atatcttcgc
     3421 taaaatcata aaattctttt tgtggttcaa caaattcata aagaatacca ttctctacca
     3481 actttgcttc aacttcgact ccattaggaa cattaaaatc tttaggtact gtcaacatga
     3541 ttgaattgcc ttgttttctt gtttttgtaa tcatttaaat ccctcatttc taacagtatg
     3601 taattactta gtaaatacat tataccacga gataatgtat ttacaagtaa ttactgtgac
     3661 tcgtaatgcg tccttgctga ataaatttca accacgtgtt catcttcatc taccttataa
     3721 acaacccggt gttgtctatt caatcttcta gaatacctac cttcatgtgt aggttttaac
     3781 ttctcaaatg attgggtagg gagataggga tcttccttta aagtctgaat aaccttctca
     3841 aattgaggtt tcaaattaga ctgtttgatt ttctttaaat cagctttagc cgagttttta
     3901 atcatcacag aatagttatt cattataagt tatcccaatc aatttcatca acattagtaa
     3961 atccactatc atctttttct cgcttacgaa caacacccaa agtgcctatt tgctcaagta
     4021 atagcgtttc tttgattgct ttccaatcat ccaaaccaat aagcaccgca ttattttcac
     4081 tgcgctcact aatgatttca atttccgtat gattctcatt tacatctttc aacaacttat
     4141 aaaaattttt ccgtgctgag ctaggattta agactgtttt tgccatatct actccccctc
     4201 taatttatat ctccattcta acgtacttaa ttaagtacgt aaattcgatt gttttgttta
     4261 aattggagta ccaaaacagc attgtaaagt gttatccgtt tcctttatac ccgtggtgct
     4321 agttaatttt ttcctagagg aaaatgtcta aacatgttgg taaatcaaca aaaaaacgca
     4381 tagagtagtc tcaaaactcc caagaaatga ggactacata tgcgttcatc aaaatatact
     4441 gaacattata cagatacttt tataacattc aaggaaatta tgagaactgt ttctaacata
     4501 taccacaatt gtgtaccaga taagattaaa aaccgaagaa atactgatca actgaagtaa
     4561 cgcgatacag tgattattgc ttgtgttata tggggcataa ttaatggcta tactagccaa
     4621 agagccacgt atagagcggt ttgttccgtc ttatttccta atggtgactt tcctagtagg
     4681 agtcgattca ctcgcttaag ttcaaactta gcttacacta tcaagattat tcgatacttt
     4741 ttcatcaaga agctcaccaa aggtgaacta gtcggaatta tagatagttt tcctagtcca
     4801 ttatgtaaac cagttagaaa tagacaagca aaactattga atcaaatagc caaagttggc
     4861 tataactcaa caaaaaaatc ttatttttat ggtcttaaaa ttcatatgat cgttactaag
     4921 actggctttc cgattactta ctcaatcacg aatccaggtg tccatgacgt gaaagtgtta
     4981 gagactttat cggaagaagc gaatcttccc aatatcctag gggacaaagg atatattagc
     5041 cataaaa
//
LOCUS       DACDIG010000119         4994 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.120, whole genome shotgun sequence.
ACCESSION   DACDIG010000119 DACDIG010000000
VERSION     DACDIG010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.120"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..382
                     /locus_tag="IV247_002716"
     CDS             <1..382
                     /locus_tag="IV247_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-dehydro-3-deoxyphosphogluconate aldolase"
                     /protein_id="HAP8778719.1"
                     /translation="NSLRYLEFREGAFDFQTSQQNLLTIPGAMSPTEIFKMFEMGADI
                     VKVFPANCLGPEYFNQVQAPLGSLPLMAVGGVDQTNAQNYLNNGASYVGIGSKFFEKS
                     AVHQLNYERLMELAESFIDSLRVE"
     gene            complement(776..1783)
                     /locus_tag="IV247_002717"
     CDS             complement(776..1783)
                     /locus_tag="IV247_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8778720.1"
                     /translation="MVTIRDIAKKSGYSTATISRLFKGDESLSITSDTKNKIIVTAKS
                     LGYDLTKIKTTLYKIAILFWISEEQLKKDVYYQHVKDDLKNYSKKANLDLIFISKNDG
                     VKSIPDDITGFIAIGQLTKKELYYLNQKGYKGVIRGINPLPEHFDTVEPDFHEMARQA
                     INYFINEGFTKIGFIGGNFFNLETGKEEIDSREYAFRYHLEKSGLLNEKYIFSQGSFT
                     VDEGYQLTLKMKAELNEDMPEACFIPSDTIAAGVLQGCHELGINIPKDMAVISINDDD
                     IAKFVAPPLTTFKIDIEEIAKTCVDLLSDQLVYSRDISKKTLIGAKLTVRKSFIPKEE
                     V"
     gene            2251..3657
                     /gene="lacG"
                     /locus_tag="IV247_002718"
     CDS             2251..3657
                     /gene="lacG"
                     /locus_tag="IV247_002718"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP8778721.1"
                     /translation="MNYKTLPEDFIMGGATAAYQAEGSTKEGGKGQVAWDTFLVEKGR
                     YTADPASDFYNQYPVDAELCKKFKIDSIRMSIAWSRIFPKGYGEVNQTGVEYYHKVFA
                     ELNKHGIIPFVTLHHFDTPKELFDNGDFLNRENVEHFVNYAQFCFDEFSEVHYWSTFN
                     EIYPVASNQYLIGSFPPEVQFDIEKTVTCLHNMMYAHAKTVNIFKDGGYKGEIGVVHS
                     LETKYPASDKEEDKHAAKLDDALSIKFLLDATYLGYYSDETMAHINEILAANGQSADI
                     DSNDFREMKKAANRNDFLGINNYGSHWVKEYKGESTLHYNTTGEKGSQVYCIKGIGER
                     IVREDLPKTDWDWIIYPKGLYDLLVRIQDEYPNYNKIYITENGLGYKDNFEDGIILDD
                     DRIDYLRQHFNAISDAIKMGVNVKGYFIWSLMDVFSWVNGYTKRYGLFYVDFDTQKRY
                     PKESAYWYKFVSETKTII"
     gene            3680..4003
                     /locus_tag="IV247_002719"
     CDS             3680..4003
                     /locus_tag="IV247_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP8778722.1"
                     /translation="MDRKELQLLGFEIVAYAGDARSSLLTVLKEVREGNFDNVERLLA
                     DADENLTLAHNSQTKCLAEEASGNEMEVGVIFIHGQDHLMTTILLRELINDFVELYKE
                     KFAKA"
     gene            4014..>4994
                     /locus_tag="IV247_002720"
     CDS             4014..>4994
                     /locus_tag="IV247_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009853324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8778723.1"
                     /translation="MNTIIKQIEKGKPFFEKISRNIYLGAIRDGFLTAMPAILFSSIF
                     IMIAAVPEVFNVTLPEAFSTWLWKVYGYSMGLVGLLVVATTARCLANSMNRQLSDSGK
                     QINEVSVMLASICGFVMMAADQIDGGIATDYLGTKGLLAAFVSAFVTVNVYKFCVKRD
                     LTIRMPKEVPGTITQMFRDIIPFSIAVFIAVLIDTGVRQFFGTSFAAGVIQLLQPLFS
                     AADGYVGIAIIWGAMAMFWFVGVHGPSIVEPAIAAIIYANVETNFELFKNGAQASKVL
                     TVGLGNFVGTMGGTGATLVVPFLFMLFAKSKQLKAVGKASFIPVSFAVNEP"
BASE COUNT         1597 a          742 c         1002 g         1653 t
ORIGIN      
        1 taacagccta agatatttgg agttcagaga gggcgcgttt gattttcaaa cttcgcaaca
       61 gaaccttcta acaattcctg gggccatgtc gcccacagag atattcaaaa tgtttgagat
      121 gggtgctgat attgttaagg tgttccccgc caactgtctt ggaccggaat atttcaatca
      181 agtgcaagcc cctttaggaa gtttaccttt gatggctgta ggaggggtag atcagactaa
      241 tgcacaaaac tatttgaaca atggagcctc ttatgtagga attggttcaa aattttttga
      301 aaaaagtgct gttcaccaac taaattacga gaggctaatg gagttggctg aatcttttat
      361 agatagttta agagttgaat agaacccatt tgcggaatac aacttgtttt ataatatggc
      421 gttatctgta taaaacatag tatcacagtg aatgagattg aactggtaga ttgatttttc
      481 gaccagttct ttttgcgcgc tctgatgctt gatatattcc caacgagata aaaaatcccc
      541 caagatacta agatgaagcg tttatccaac ctcatactta gataaaaagg aggaaatgtt
      601 ctgaatgata aacaaaaatt agcgcgcaca atttggcgat gaaaaaatca tattgtattt
      661 acgtcaaaat atcaccatca aattatttat ggaaaacata ataggcataa acccgattgc
      721 tcggctacta tgtggacaca attatagttt taaagataag tttcaattac ttattttaca
      781 cctcttcttt agggataaaa cttttcctaa cggtcaattt tgcgccgatt aatgttttct
      841 ttgatatgtc ccgcgaatat accagttgat cacttaataa atctacacag gttttagcta
      901 tctcttcgat atctatctta aaagttgtaa gtggtggtgc aacgaatttt gcgatgtcgt
      961 catcattaat acttattact gccatatctt tagggatgtt aatacccaat tcatggcagc
     1021 cctgaagaac gccggcagct atagtgtcag atggtatgaa acatgcctcg ggcatatctt
     1081 catttaactc agctttcatt ttaagagtta attgataccc ttcgtcaaca gtaaatgatc
     1141 cttgggagaa aatatacttt tcattgagta gtccgctctt ttctaaatga tatcggaagg
     1201 catattcacg gctatcaatc tcttctttac ccgtttctag gttaaagaaa tttcctccaa
     1261 taaatccaat ttttgtgaaa ccttcattaa taaagtagtt aattgcttgt ctggccattt
     1321 cgtggaaatc aggttctaca gtatcgaagt gttcaggtaa tgggtttatt cctcggatta
     1381 cccccttata ccctttttga tttaagtagt acaattcctt ttttgtcaat tgtcctattg
     1441 cgataaatcc agtaatatcg tcaggaatgc tttttacacc atcgttttta gagatgaaaa
     1501 tcaaatctag attagctttt ttactataat ttttcaaatc atctttgaca tgttgatagt
     1561 agacatcctt ttttaactgt tcctctgaga tccaaaaaag aatggcaatc ttgtacagag
     1621 tagtcttaat ttttgtcaga tcatatccta aagatttggc tgtaacgatt attttgtttt
     1681 tagtatcaga tgtaatagag agtgactcat cacctttaaa aagtcttgat atagtagccg
     1741 ttgagtaacc agattttttt gcaatatctc taatagtaac cattgttcat cctcctaggg
     1801 atataataat ccttttcttt gttaaagtaa atcgaaactg ctcttttagt aacttaaatt
     1861 actttttcta gcaggaaacg aaactctgat atcagcagag agcctaatcc attaacagag
     1921 aagattgaat cgataatgga tagccacttg gagaatgatt agtaggtggg attgatttta
     1981 gtgggaatca gtccgttttc ttgtttcgtg taaattaaat tagtaaaaag ataaatttat
     2041 tttacaaaaa aaacgtttac aaaacggttt ttgaatggta tatttatttt acatcaaggg
     2101 atataagtgc ttgtatatcg caataaaata gatattcgag gtcggttttt cgttgtgtaa
     2161 aaaaatttac ttatacatgt taaaattatg ctgaggtgat ttatggatta ttggcatatt
     2221 gatcaaacta attttcaagg agagattaaa atgaattata aaactttacc agaagacttt
     2281 attatgggag gagctacggc agcttatcaa gcagaaggct ctacaaaaga aggtggaaaa
     2341 gggcaagtag cgtgggatac tttccttgta gaaaagggac gctatactgc tgatccagct
     2401 agtgattttt ataatcaata tccagttgat gctgaattat gcaaaaaatt taaaattgat
     2461 agtattagga tgtctattgc atggagccgt atttttccaa aaggctatgg ggaagtgaac
     2521 caaacaggag tggaatatta tcataaggta tttgctgaat tgaataagca tggtattatt
     2581 ccttttgtaa ctcttcatca ttttgatacc cctaaagaac tatttgataa tggagatttt
     2641 ttaaatcgag aaaatgttga acatttcgta aactatgcac agttctgttt cgatgaattt
     2701 tctgaggttc attattggag tacatttaat gagatttatc cagttgcttc aaatcaatat
     2761 ttgatcggat cgtttcctcc agaagttcaa tttgatatag agaagactgt aacttgttta
     2821 cacaacatga tgtatgcgca cgcaaagaca gtaaacattt ttaaagatgg tggatataaa
     2881 ggggaaattg gtgttgttca ctcgctagag acaaaatatc cagcaagtga caaagaagaa
     2941 gataagcatg ctgcgaaatt agatgatgcg ttatcaatta agttcttatt agatgctact
     3001 tatttagggt attactcaga tgaaacaatg gcacatatta atgagatttt agcagcaaat
     3061 gggcaatctg cagatattga ttctaatgat tttagagaga tgaaaaaagc agcaaatcga
     3121 aatgattttc ttggtatcaa taactatggt agccattggg ttaaagagta caaaggagaa
     3181 agcacacttc actacaacac taccggagaa aaaggtagcc aagtctattg cattaaaggg
     3241 attggcgaga gaatcgtgcg tgaggatctt ccgaaaaccg attgggattg gatcatttat
     3301 ccaaaagggt tatatgattt acttgtacga atccaagacg aatatccaaa ttataataaa
     3361 atatacatta ctgaaaacgg gctagggtat aaggataatt ttgaagatgg gattattcta
     3421 gatgatgatc gaatcgatta tttacgccag cattttaatg cgatttccga tgctatcaaa
     3481 atgggagtta atgtaaaggg gtatttcatt tggtcattaa tggatgtttt ctcttgggtt
     3541 aatggataca ctaagcgtta tggactattc tatgtagatt tcgatacaca aaaaagatat
     3601 ccgaaagagt ctgcatattg gtataaattt gttagcgaaa caaaaacgat aatataaaaa
     3661 tagttaggag ggcaaggaaa tggataggaa agaattgcaa ttattaggat ttgaaattgt
     3721 tgcttatgca ggagatgcca gaagttcatt attaacagtg ttgaaggaag tgagagaagg
     3781 taattttgat aatgttgaac gattattagc ggatgctgat gaaaatttaa ccttagcgca
     3841 taactcacaa acgaaatgtt tagctgaaga agcgtcaggg aacgaaatgg aagtaggcgt
     3901 aatttttatt catgggcaag accatttaat gacaacgatt ctattaagag aacttatcaa
     3961 tgattttgtt gaattgtaca aagaaaaatt tgctaaagca taggagaaga aaaatgaata
     4021 cgattattaa gcaaattgag aagggaaaac cgttttttga aaaaatctca cgtaatattt
     4081 atttaggggc aattcgcgat ggctttttaa ctgctatgcc agctattcta ttttcaagta
     4141 tttttattat gattgcggct gttccagaag tatttaacgt gacgttacca gaggcattct
     4201 cgacgtggct ttggaaagtt tatggttatt caatgggatt agttggtctt ttggttgttg
     4261 ctacaacagc tcgatgttta gctaattcaa tgaaccgtca attatctgat tcaggtaagc
     4321 agattaatga agtttctgtt atgttagcgt caatttgtgg ttttgtaatg atggcagctg
     4381 atcaaattga tggtgggata gctactgatt atttagggac aaagggatta ttagcagctt
     4441 tcgtttctgc gtttgttact gtaaatgtgt ataaattctg tgttaaaaga gatttgacga
     4501 tccgtatgcc aaaagaagtt cccggaacaa tcacacagat gtttagagat attatcccct
     4561 tttcaattgc agtatttatt gccgttttga ttgatacggg agttcgtcaa ttttttggaa
     4621 catcatttgc agcaggtgta attcaattat tgcagccatt atttagtgct gctgatggat
     4681 atgtcggtat tgcaatcatt tggggagcca tggcaatgtt ctggtttgtt ggtgttcatg
     4741 gaccatcgat tgttgaacca gcgattgctg caattattta tgcgaacgta gaaactaact
     4801 ttgaattatt taaaaacggt gcacaagcct caaaagtatt aacagttggt ctaggaaact
     4861 ttgttggaac gatgggtggt actggagcga cactagttgt tccttttctg ttcatgttgt
     4921 tcgctaaatc aaaacaattg aaagcagttg gtaaagcttc atttattcca gtttcgtttg
     4981 cggtaaatga accg
//
LOCUS       DACDIG010000120         4955 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.121, whole genome shotgun sequence.
ACCESSION   DACDIG010000120 DACDIG010000000
VERSION     DACDIG010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4955
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.121"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            70..555
                     /locus_tag="IV247_002721"
     CDS             70..555
                     /locus_tag="IV247_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAP8778724.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(659..1252)
                     /gene="clpP"
                     /locus_tag="IV247_002722"
     CDS             complement(659..1252)
                     /gene="clpP"
                     /locus_tag="IV247_002722"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAP8778725.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            1432..1959
                     /locus_tag="IV247_002723"
     CDS             1432..1959
                     /locus_tag="IV247_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAP8778726.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            2050..2787
                     /locus_tag="IV247_002724"
     CDS             2050..2787
                     /locus_tag="IV247_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP8778727.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            2791..3708
                     /gene="miaA"
                     /locus_tag="IV247_002725"
     CDS             2791..3708
                     /gene="miaA"
                     /locus_tag="IV247_002725"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAP8778728.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            3710..4939
                     /gene="hflX"
                     /locus_tag="IV247_002726"
     CDS             3710..4939
                     /gene="hflX"
                     /locus_tag="IV247_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAP8778729.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         1687 a          849 c         1110 g         1309 t
ORIGIN      
        1 aaatatatgc tatgatacct ttattcaata ggattagttt ctacaaaaaa tgaaaggaga
       61 aaaataaata tggaacggtt tatagcatca aatgccacag tgattggtga tgtgacattg
      121 tcagaagatg tgacgatctg gtatcaagct gttttacgcg gggacagtaa ttggataaaa
      181 ataggacaaa gaacgaatat ccaagatggt acaatcatcc atgtcgatca tgatgcacct
      241 gtagatattg cggagaatgt gacggtagga catcagtgca tgctacatgg ctgtacaatc
      301 gaaaaagggg cgctgatcgg gatggggacc acgatattga atcatgcggt tattggtgag
      361 aatagtttga ttggtgctgg atcattagta acagaaggaa aagttattcc tcctaatgtc
      421 ttggcttttg gtcgcccagc taaagtgatc cgtcctttga caaaagaaga aatacaaaaa
      481 aacaaagaaa acatccagca ttatgtcgag ataggtcgga tgcatgcgca aggaaaattc
      541 aaggagcaaa gataggcagt agcatccata aaataaaaaa ggatggtcag tttgagttga
      601 ccatcctttt ttatttggtt tagataagag agcttaaaaa agcttccttt gaaacagatt
      661 aatttaatga agaactgttt tccatgactt catcgatcaa tccatatgct tttgcttgtt
      721 cagctgtcat atagttgtca cgatctgtat ctttttcaat cacttcaagc ggttgtccag
      781 tgcgttcagc taaaattttg tttaagcgtt cgcgtgtttg caaaatatgg cgtgccgcaa
      841 tttcgatttc agttgcttgc ccttgagcgc cgccaagagg ttgatggatc atgatctcag
      901 catttggtaa agcaaatcgt ttaccttttg taccagctgt cagcaagaag cttcccattg
      961 aagcagccat acccataaca attgtttgga catctgcttt gacaaagttc attgtgtcgt
     1021 aaatagccat acctgctgta actgaaccac caggagaatt gatataaaga tagatatctt
     1081 tttcagaatc ttgagcgtct aagaaaagca attgggcgat gatcgaatta gcaagatcat
     1141 ctgttacttg gccgcttaac atgatgatac ggtctttcaa taaacgtgag tagatatcgt
     1201 aagctctttc acctcgtgaa gattgttcaa tgactgtagg aattaaattc ataatagagt
     1261 tcctccttga tttcgtttag aaatagtgta ccataattta gtttctaata cctaaaatgt
     1321 agtatacatt tttggtcaaa aaaggtcaaa taaaaaactt aggcaaatta gtcgcataaa
     1381 cccgctttat ggtatgataa gcagagaaac aaaacaagga ggaacaacag attgatagtt
     1441 ggtattactg gccttatcgg agtgctgaca ggtgctgtag ttgtctatat cggcatgata
     1501 ctgcaaatgc gtcacttgga aaataaagaa cagcgtagac gtgaattgga attgaaagtc
     1561 aaagaaatcg agacgctgaa tcaattaaat aaaaagatca acgagatctt gcaaaaacgg
     1621 acaacgctga tggaagaata tgtcagcttt gatgcatttg atgattgtta tatcacgatt
     1681 gacgattttg cgtatctcca atcattttca gcgcaaaata gtttttatct gccaaattat
     1741 attttagacg agctatttaa gaatatctcc catcgaaaag tgattttatc tccagatgaa
     1801 acaatgaaga ttggtggata tgcttataaa ggcggacgcg tgattttaga gaattttacg
     1861 gatagtctga ctgaaatgat caatgaaaaa aaagctcagt tgaagactat gacgcgccag
     1921 ccgattggtt tcttctcgaa agatgatgtt tggagctgat attttgaagc attgccgttg
     1981 cacatgagag cgtggtaatg ctttcttttt ttcttaatga tacaatagaa ctgaacggag
     2041 tgaagaaaga tgttacaaat ctatgcccat cgcggaagca gcggcaccca cccagaaaat
     2101 acgcttcctg catttgcaga agctgtacga gtaggtgcag atggtatcga actagatgtc
     2161 catctaagca aagatggtta tttgatcgtt atgcacgacg aagaagtgga ccggacgact
     2221 aacggtaaag ggctgattcg agaaaaaaca ttggaagaac tcaaaaaatt gaatgcaggg
     2281 ggcaaattca gcaaagaatt tccagcagct aaagttcctg aattgagaga agtgttagga
     2341 ctgctcctcc aaaaaaatta ccgagggatg ctaatgattg aaattaaaac tgatcagtat
     2401 gaatatcccg gtatcgaaga aaaattagcc gaaacattaa aaagtagaga ttggcctttt
     2461 caacatgcat attgcagctt tcatttggat tctttggaaa aaatcgcatt actggaaccg
     2521 gagatacagc tggacttatt gatgagtact tctgataaaa agatccaact agcagcagaa
     2581 accccttata tagaaggagt acatccaaaa aatacttggt tgttcgaaca tccagtccag
     2641 gcagctgatt ttccaaaagc aattcgtgca tggacgatta acgatgaaga agaaatggcc
     2701 aaatgtcaag aactcaaaat agatggaata ataactgatt ttccggagaa agctcttcgg
     2761 tggaaacaat caacgaaaag taggtaaaat atggaaaaag tattagtgat cgtcggtccg
     2821 acagcagtcg gtaaaacagc gctaagtgtg gaactcgcac aactatttgg aggagagatc
     2881 atcagtggtg attcattgca aatctataaa aatctggata tcggtacagc aaaaatcaaa
     2941 gaagaagaga tgaataatgt tccgcatcat ttgattgatg tgatcgaacc tactcaacgt
     3001 tactcagccg ctgattttca gcattcaggt cgacaattga ttaccgagat tgcgaaccga
     3061 gggcacctac ctatcatagc tggtggtacg ggtctctata tccaatccct tctatatgat
     3121 tatcagttag gagcgacaga agaagcgaat cctgagatta gaaaaaagta tgaaacgtta
     3181 gctgaaacaa ttggttccca agcattatgg aatcagttga aagaaaaaga cccggcagct
     3241 gctgagaaaa tccactggaa caatcaacgc aaagtgataa gagctctgga agttttagag
     3301 acgacaggac atagtatcac ggcacctaaa gaagagccaa agcaattgta tgattacctc
     3361 atgattggat tagatacgaa gcgtagtatt ctttatgaac gaatcaatca aagagtcgat
     3421 ttgatgctag cagaaggttt agtagcagaa gcgcgtaagg tgtatgagct tggagaagtc
     3481 caggccagtc aaggtattgg atataaagaa tttttccctt attttgaagg gaaagaaaca
     3541 ttggaagaag caacagaaca agtcaaacta cattcgagaa gatacgcgaa acgtcagttg
     3601 acgtggtttc gtaaccgttt ggatgcacag tggtttgatc ttataagtga gccaaatcaa
     3661 aaaagtcaga ttgagcaaac aatcaaaaaa tggctggagg cggaataaaa tggaagaaaa
     3721 agtgatcatc gtaggggtag aaaccgaagc aaaccagcgt tacttttcag agtcaatgga
     3781 agagctagtt cagctgacga ataccgcatc aggagaagtc gtattcacaa ttacacaaaa
     3841 gagaccacaa gtggatcggc aaacgattat cggtaaaggg aaattacaag aattagttca
     3901 acaagcggat gcgcatgaag cggatttgat catctttaat catgagctaa ctccgagaca
     3961 gagccaactg attaccgatg ctgttggtct tccggtaatt gatcgcgtgc agcttatctt
     4021 agatattttt gcgatgcgtg ctcgttcgaa agaagggaag ctgcaggttg aacttgctca
     4081 gttagaatac ctgctgcctc gtttagtagg gcaagggaaa acattatctc gtcttggcgg
     4141 tgggatcgga acaagagggc caggtgaaac aaagttagaa acagaccgcc gacatatacg
     4201 gaataaaatt tttgctgtta agaaagaatt gaaagaagta gaagctcatc gagagcgcaa
     4261 tcgccaaaaa agaaaaaata gcgagatttt tcagattgga cttattggct acacgaatgc
     4321 cgggaagtca actatcttga atctattgac acaagcggat acttattcga aagatcaatt
     4381 gtttgcaacg ttagatccat tgactaagag atggcgtttt gctgaaggat ttgagattac
     4441 tgtaacggat accgtcgggt tcatccaaga cctgcctacg caactgatcg atgctttcca
     4501 ttctacatta gaagaaagtc aaaatatgga tcttttgcta catgtagtcg atgctagttc
     4561 tccagatcgt attttacagg agcagactgt cttaaaattg atggatgaac tgaatatgaa
     4621 agaaatgccg atactgactg tttacaataa ggcagaccag atcgacccag cgatgttcac
     4681 gcctacgcta ttcccaaatg tattgatttc tactcagacg aaagaaggta agatggcgtt
     4741 gatcgaagca atcaaacgtc agctgatgga actaatgacc ccttatacac tttctgtgcc
     4801 aagcaacgaa ggacagaaat taagcgagtt gagaagacaa acaatggttt tggacgaagc
     4861 gtacatggaa gaaacaaacg aatatgaagt taaagggttt gcaaaaacca attcaaaatg
     4921 gttaagaaaa aaccaataaa attttatttt tttaa
//
LOCUS       DACDIG010000121         4738 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.122, whole genome shotgun sequence.
ACCESSION   DACDIG010000121 DACDIG010000000
VERSION     DACDIG010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.122"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(238..426)
                     /locus_tag="IV247_002727"
     CDS             complement(238..426)
                     /locus_tag="IV247_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778730.1"
                     /translation="MSYTLQQEHQILGLIKQRRKQLQDDRAALRKSDELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            complement(447..731)
                     /locus_tag="IV247_002728"
     CDS             complement(447..731)
                     /locus_tag="IV247_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778731.1"
                     /translation="MQITIPDNLVVSELTTQITNAVLNSLDERLHLMNKSVELPPYPN
                     KSEVKKVLGIGDDKLTHWISLGLKTQQWSKLDIRIERSELQRFLKENFEF"
     gene            complement(733..927)
                     /locus_tag="IV247_002729"
     CDS             complement(733..927)
                     /locus_tag="IV247_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8778732.1"
                     /translation="MTMSQEFILKVRIQLAKYGKSQNWLADTIGISRPYMSDIMNGRR
                     KPDKQIKPIEAALAELEKEK"
     gene            complement(924..1700)
                     /locus_tag="IV247_002730"
     CDS             complement(924..1700)
                     /locus_tag="IV247_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP8778733.1"
                     /translation="MNTPQIFNFEQHEVRTVTIHDEPFFVGKDVAKVLGYQNGSRDIN
                     RHVDVEDRQNYQNGTFESPRGLTIINESGLYSLILGSKQPNAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATKLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMRQGYTKSHTTEIPK
                     SDGGTKVVMNTKWTQKGRLFIYESLKKEGYIPEIDLLEEE"
     gene            complement(1827..2048)
                     /locus_tag="IV247_002731"
     CDS             complement(1827..2048)
                     /locus_tag="IV247_002731"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778734.1"
                     /translation="MKQNKKTPSAKGALITLNFKDQKFSAVVDEIHPQEHQKNSFATD
                     EKKTQKRSEKVLRIIQFLLEKIRHFGSLF"
     gene            2349..2693
                     /locus_tag="IV247_002732"
     CDS             2349..2693
                     /locus_tag="IV247_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8778735.1"
                     /translation="MLFERVKELCKKRGISISELENRIGFGKNSIYKWKTQSPKIETL
                     QKVADYFHVSTDYLLGRTDDPNAGVAPEERKLTVEEALASVMSSDGKPLTDNDREILS
                     AMIEAYLEKKDN"
     gene            2729..3124
                     /locus_tag="IV247_002733"
     CDS             2729..3124
                     /locus_tag="IV247_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP8778736.1"
                     /translation="MIINELGVKVEERENLDADGHYVACMNTIVIKANLSKYRRQRTL
                     LHELGHASKHHDNYFLYNLAFSLHSKMEYEADRFMIEKLLDRYIAKSELEPHNINYMK
                     FIEDNNLSVRFEPLVKELLKARIYCYAAL"
     gene            3177..3488
                     /locus_tag="IV247_002734"
     CDS             3177..3488
                     /locus_tag="IV247_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778737.1"
                     /translation="MIYTEFKEWLEKSTTGYETFIIKATNYQIEKNKNRPQKKRWDDK
                     KIDKAVLEMWKQVVTNLYQTIRKEKGVPLINGKEIWLEFIEEQGLIEFFNDSMAELEF
                     E"
     gene            3500..4648
                     /locus_tag="IV247_002735"
     CDS             3500..4648
                     /locus_tag="IV247_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8778738.1"
                     /translation="MAMIKQYKKKNGEKAWYFKTYLGIDPLTGKKKYTTKRGFRTQKE
                     AKTALSRLELELQKTGMPTSTNTTFKEAAELWLESYKKTVKESSYSRTKIIFNKHIYP
                     KFGNIKLSKINTAYCQKVVNDWSEKGTSKQYPLFINYMNKVFKYAINIGLTSDNPTLN
                     LLIPKPQIKTEKKLKLYTKEQLELFLNEVSQEQNPYFKNRDYTLFRLLAFSGCRIGEI
                     LALTWDNINFKTNEMAIKKTVARSDKYYISETPKTKKSNRIIYLDEKTIKQLKFWKLE
                     QRKYLFQLGFTKANYLFTNDENNFTINQSVAERYNIYRERAGLPYIGLHGFRHTHASM
                     LYEAGADHKEVQERMGHANIKTTMDTYTHITNSKKEETTQKLTNYINF"
BASE COUNT         1654 a          788 c          753 g         1543 t
ORIGIN      
        1 cttttcgata tgttgcttgc ttaaaataat agaacggcta tttgcccacc aattatcagc
       61 aatcacttta ccgattttta gcgcttcttc tcgtgccata gttgctcctt tcttttgagt
      121 caagcagatt gattaaaacc atcaatgctg cgaacaagct tcctccgata atactttggt
      181 gcgctacaat cactaataac cccaaaatga atcctataaa aagtgtgtct gtcttcttca
      241 taatctaatc tccctatttt ttatttctag cattctcaaa tcctcaagtt cagaagcaat
      301 tagttcagct tgtctatctg atagctcatc ggattttcta agcgctgcac gatcatcttg
      361 taattgtttt ctacgctgtt taatcagacc tagaatttga tgttcttgtt gcaatgtgta
      421 ggacataaaa tcattctcct ttgcctttag aactcaaagt tttctttcaa aaatctttgg
      481 agttccgatc gttcaattct gatgtctaac ttgctccact gctgtgtttt taagcctagg
      541 cttatccaat gtgttaattt gtcatcacca atgcctaaaa ctttttttac ctctgatttg
      601 tttggatatg gaggaagctc tactgatttg ttcataaggt gtaatcgttc gtccaatgaa
      661 ttaagaacag cattcgtaat ctgtgtagtt aattcggaaa ctactaaatt atctggaatt
      721 gttatttgca tgttatttct ccttctctaa ctctgctagt gctgcctcaa ttggcttgat
      781 ttgtttatct ggttttctac gcccattcat aatgtccgac atatatggtc tagaaattcc
      841 gatagtatca gctaaccagt tctgagactt tccgtatttt gctaattgaa ttctgacttt
      901 caaaataaat tcttgtgaca tcgtcattcc tcctctaata aatcgatttc tggaatatat
      961 ccttcttttt tcagcgactc ataaatgaac agacgccctt tttgagtcca ttttgtattc
     1021 atcacaactt ttgttccgcc atcagatttc ggaatctcag ttgtatgaga ttttgtgtaa
     1081 ccctgtctca tgtgtttttt gcataataac cattggttgc ctactttttt ctgaatacct
     1141 agcttatgaa gtagtttgtt catctgttgt ggagacatcc cataatcagc cgcgatttga
     1201 ctaatagtta ctgaatctgt agaagataag atactatcca aatacgaaat tttgggttcg
     1261 tattctgcaa ttttttgttc tgcgattagt cttccagtac gttcttcttt caacttagtt
     1321 gctaattgga tgattgtatc tggattaagc aaagcttctt ctactttttc tggagttaga
     1381 taacctccat gttttctaat tgctggcaac acttcgctag tcacccaacg tttaaatttc
     1441 ttagcatttg gttgtttgct tcctaaaatt aaggagtaca aacctgattc gttaataata
     1501 gttaaccctc taggcgattc aaaagtaccg ttttggtagt tttgccgatc ttctacatcg
     1561 acgtgtctgt taatatctct actaccattt tggtacccta gaactttagc cacatctttt
     1621 ccaacaaaaa atggttcatc atgtattgtt actgttctta cttcatgttg ttcaaagtta
     1681 aaaatttgcg gtgtgttcat ttttgctcat tcctttcttt gatataattt tgaatagaaa
     1741 gcgaggtgaa aatagtatgg aagaatttaa tatggatgtc gacgccttat tcaaacaaac
     1801 cgtattcaag actgttaata aagaattcaa aatagacttc caaaatgacg aatcttttcc
     1861 aacagaaatt gaattattcg aagaactttc tcagagcgtt tctgagtctt tttctcgtca
     1921 gttgcaaaag aatttttttg atgctcttgt ggatgaattt catcaacaac agcactaaac
     1981 ttttggtctt taaaatttaa agttattaaa gctccttttg cggaaggagt ttttttattt
     2041 tgtttcatga tgtttcctcc ttttctttaa atatgtaagc taataaaatt agctaatttt
     2101 tattgacatt tctatccaac tgtcttatat ttatatacag ttaaataagc ctagaaaaac
     2161 cctaaaaatt aacattctta gtttgccgac ctcgaattcg tttattttat taggtgtatt
     2221 tcttgttgct tgttagctta ttaaattagc ttacgagtta aatataagac aattggatag
     2281 atttgtcaac tacctttcta tctttttgta taaattatta tcgtacgctc taggaaggtt
     2341 gatttgacat gcttttcgaa agggttaaag agctctgtaa aaaaagaggt atttctattt
     2401 ctgaattaga aaatagaata ggatttggaa aaaactctat ttataaatgg aagacacaaa
     2461 gtcctaaaat agaaactttg caaaaagttg ctgactactt ccatgtatca acagattatt
     2521 tgctaggacg cacagatgat cctaacgcgg gagttgcacc agaggaaaga aaactaaccg
     2581 tggaagaagc tttagcatct gttatgagta gcgacggaaa accgctcacc gataatgata
     2641 gggaaatact atcagctatg attgaagcat atttagagaa aaaagataac taaataagta
     2701 ggtgagtcat ttggacagtc aaattgaaat gataattaat gaactcggcg ttaaggtaga
     2761 agagcgtgaa aaccttgatg ccgatggcca ttatgttgct tgtatgaata ccatagtaat
     2821 aaaagctaat ttatctaagt atagaagaca aagaacctta ttacatgaat taggacacgc
     2881 ttctaaacat catgataatt attttttata taacttagca ttctctctcc attcaaaaat
     2941 ggaatatgag gctgatcgct tcatgattga aaaattatta gatagatata ttgcaaagtc
     3001 tgaattagaa ccacacaata tcaattacat gaaatttata gaagataaca atttaagcgt
     3061 tcgcttcgaa ccacttgtga aagaattatt aaaagctcgc atctattgtt atgcagctct
     3121 ctaaaatttt ttaagcaaaa aagaacatac gttcaaaaat agaaaggtga acaaaaatga
     3181 tatatacaga attcaaagaa tggttagaaa aaagcacaac cggatacgaa acatttatca
     3241 tcaaagctac taattatcaa attgaaaaaa acaaaaatag accccaaaaa aaacgctggg
     3301 atgataagaa aatagataaa gctgtattag aaatgtggaa acaagtcgtg actaacttgt
     3361 atcaaacaat tcgtaaagaa aaaggagttc cattaattaa cgggaaggaa atatggcttg
     3421 aatttataga ggaacaagga ctgatcgaat ttttcaatga tagcatggca gaattagaat
     3481 ttgaataggg gtaatattga tggcaatgat aaaacaatat aaaaagaaaa atggcgaaaa
     3541 agcatggtac tttaaaactt atctcggtat tgatccgcta actggaaaga aaaaatatac
     3601 tactaaacga ggatttagaa cacaaaaaga agcaaaaaca gcactttcta ggttagaact
     3661 agaattacaa aaaacaggaa tgcccacaag tacaaatact actttcaaag aagcagcaga
     3721 attatggcta gaaagctaca aaaaaactgt aaaagaaagt tcatattcaa ggactaaaat
     3781 aatctttaat aaacatatat atcccaaatt tggaaatatt aagctttcta aaattaatac
     3841 ggcatattgt caaaaggtag taaatgattg gagtgaaaaa ggaacttcaa agcagtaccc
     3901 tcttttcata aactatatga ataaagtttt taagtatgct ataaatattg gtttaacatc
     3961 tgataatcca acattaaatt tacttattcc aaagccacaa attaaaacag aaaagaaatt
     4021 aaaattatat acaaaagaac agttggaatt atttctaaat gaagtatctc aagaacagaa
     4081 tccatatttt aaaaacagag actatacgct ctttagacta ttagcattca gcggatgtag
     4141 aatcggcgaa atattagcac tcacttggga caatattaat tttaaaacaa atgaaatggc
     4201 cattaaaaaa actgtagctc gttcagataa atattatata tctgaaactc ctaaaaccaa
     4261 aaaatcaaat cgaataattt atttagatga aaaaactata aagcaactaa aattttggaa
     4321 gctcgaacaa agaaagtact tatttcaatt aggatttact aaagctaatt atttgtttac
     4381 caatgacgaa aataatttca caattaatca gtcagtggca gaaagataca atatatatcg
     4441 tgagcgtgcc ggcttacctt atatcggtct gcatggtttt agacatacac atgcatcaat
     4501 gctatatgag gcaggcgcag atcacaaaga agtccaagaa agaatgggcc acgcaaatat
     4561 aaaaactact atggacacat atacacacat tactaacagc aaaaaagaag aaacaacaca
     4621 aaaactaaca aattatatta acttctaaga aagtatggtc aaaagtatgg tcaaaaaaaa
     4681 tcccataatt tacaaaaaag ctccatccct tgctacacaa gagatagagc agtttatt
//
LOCUS       DACDIG010000122         4711 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.123, whole genome shotgun sequence.
ACCESSION   DACDIG010000122 DACDIG010000000
VERSION     DACDIG010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.123"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            106..357
                     /locus_tag="IV247_002736"
                     /pseudo
     CDS             106..357
                     /locus_tag="IV247_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            450..1244
                     /locus_tag="IV247_002737"
                     /note="aph(3')-IIIa"
     CDS             450..1244
                     /locus_tag="IV247_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP8778739.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1654..1765
                     /locus_tag="IV247_002738"
                     /pseudo
     CDS             1654..1765
                     /locus_tag="IV247_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787626.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            1786..1869
                     /locus_tag="IV247_002739"
     CDS             1786..1869
                     /locus_tag="IV247_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAP8778740.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1994..2731
                     /gene="erm(B)"
                     /locus_tag="IV247_002740"
     CDS             1994..2731
                     /gene="erm(B)"
                     /locus_tag="IV247_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP8778741.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(2994..3857)
                     /locus_tag="IV247_002741"
     CDS             complement(2994..3857)
                     /locus_tag="IV247_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HAP8778742.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3859..4131)
                     /locus_tag="IV247_002742"
     CDS             complement(3859..4131)
                     /locus_tag="IV247_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP8778743.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4148..4363)
                     /locus_tag="IV247_002743"
     CDS             complement(4148..4363)
                     /locus_tag="IV247_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HAP8778744.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(4455..>4711)
                     /locus_tag="IV247_002744"
     CDS             complement(4455..>4711)
                     /locus_tag="IV247_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HAP8778745.1"
                     /translation="IGFVPYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVS
                     TWSKNGITEHKGYDKKVLSMYKNVFFEMLERIIQLENEKE"
BASE COUNT         1446 a          796 c          865 g         1604 t
ORIGIN      
        1 ttcttgggag ttttgagact actatatgcg tttttttgtt gatttaccaa catgtttaga
       61 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
      121 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      181 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      241 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      301 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      361 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      421 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      481 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      541 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      601 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      661 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      721 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      781 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      841 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      901 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      961 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
     1021 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1081 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1141 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1201 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1261 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1321 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1381 cgcctacgag gaatttgtat cgataagaaa tagatttaaa aatttcgctg ttattttgta
     1441 catttaactt gacggtgaca tctctctatt gtgagttatt agtggtacag ttttcaaccg
     1501 ttttaattat aaaaaagtgg tgcattttta aattggcaca aacaggtaac ggttattgca
     1561 ggtgtatttc ttatctatgg gtttaacatg gattttatca ttaaaatcat gagtattgtc
     1621 cgagagtgat tggtcttgcg tatggttaac cctaaagtta tggaaataag acttagaagc
     1681 aaacttaaga gtgtgttgat agtgcattat cttaaaattt tgtataatag gaattgaagt
     1741 taaattagat gctaaaaatt tgtaattaag aaggagggat tcgtcatgtt ggtattccaa
     1801 atgcgttatg tagataaaac atctactgtt ttgaaacaga ctaaaaacag tgattacgca
     1861 gataaataaa tacgttagat taattcctac cagtgactaa tcttatgact ttttaaacag
     1921 ataactaaaa ttacaaacaa atcgtttaac ttctgtattt gtttatagat gtaatcactt
     1981 caggagtgat aacatgaaca aaaatataaa atattctcaa aactttttaa cgagtgaaaa
     2041 agtactcaac caaataataa aacaattgaa tttaaaagaa accgataccg tttacgaaat
     2101 tggaacaggt aaagggcatt taacgacgaa actggctaaa ataagtaaac aggtaacgtc
     2161 tattgaatta gacagtcatc tattcaactt atcgtcagaa aaattaaaac tgaatactcg
     2221 tgtcacttta attcaccaag atattctaca gtttcaattc cctaacaaac agaggtataa
     2281 aattgttggg aatattcctt accatttaag cacacaaatt attaaaaaag tggtttttga
     2341 aagccgtgcg tctgacatct atctgattgt tgaagaagga ttctacaagc gtaccttgga
     2401 tattcaccga acactagggt tgctcttgca cactcaagtc tcgattcagc aattgcttaa
     2461 gctgccagcg gaatgctttc atcctaaacc aaaagtaaac agtgtcttaa taaaacttac
     2521 ccgccatacc acagatgttc cagataaata ttggaagcta tatacgtact ttgtttcaaa
     2581 atgggtcaat cgagaatatc gtcaactgtt tactaaaaat cagtttcatc aagcaatgaa
     2641 acacgccaaa gtaaacaatt taagtaccgt tacttatgag caagtattgt ctatttttaa
     2701 tagttatcta ttatttaacg ggaggaaata attctatgag tcgcttttgt aaatttggaa
     2761 agttacacgt tactaaaggg aatgtagata aattattagg tatagggcac ctctaataac
     2821 taccaattaa ttcacctcaa aatgaaatta gaaaaaaaca gcgaaatttt taaatctatt
     2881 tcttatcgat acaaattcct cgtaggcgct cgggacccca acatgaggaa atgtttttat
     2941 aaaaaaagag tagttaccgt tttttggtaa ctactctttt ccttttttaa ggtttaaata
     3001 cctggaagtt taggtgtttt gggtattggt ggtgtaggtg gctgcaagct ttccaatttt
     3061 tgttgaattg ctttaaattc aggtgtctct tggtgtttat ttagaaccat tttttgctct
     3121 attcgttcta aagtcggttg aatttctttc cctgatactt tacgattcaa ttctttttct
     3181 aaggtttctt ttggactaat agaaggtgtt tccaaacttg aatagagttt tactccttct
     3241 ctgttatata gccttatatc gctaaataag cccgttttat gaagggtttc taaattggtc
     3301 ggtaagtttt tgacaacaat atcatgcgct tgttttggtg ttgccctggc tgtcattgga
     3361 tcatctgcat acatggtttc atatcgttca attgttccta aatatgagtt gattttaggt
     3421 actgccatga catacatttt tgtttcataa cctttggctt gaagcattgt tgcggtttga
     3481 ataggaacgt ctgttgttcg tcctgtacct tcgatcacca aattataccc ttgatcgctc
     3541 aaacggctta tgatcgcttc tgtcatgcga ttagaataag gggtaacgtg ttttactacg
     3601 tctttttcat aaagtttcac tagttcatca aaattagggt gctgttgttt aaaggtatca
     3661 ttatcaatga caataacatt cccttgtgtt tcttcaaaaa ttgctgatcg caaactggtt
     3721 ttccctgacc ctggttgccc accaagtaaa aaagcggttg gcgattcaac cgcttttttt
     3781 ccttgaatca attcttctaa attatcattt aagcgattct caaattgttt gtcagtaaaa
     3841 ttgactatat ttgccatatt aagccacttt ctctttattc aaaacttgtt ttgagtaacg
     3901 attcattgac cgccaatact catgaacggc ttgtaattct tctaaagaat aatcaaaaag
     3961 atttacacgt tttgcaagct ttaattggtt taataaattg acttccaata attgagagtc
     4021 atttttattt aattcatggt tcaaaacata gtttaatact cgattataaa cgcttgccga
     4081 taattcgtta atgatctcta tttcaaatgt tttttcatac gtaactgcca tttcattttc
     4141 actcctttta aagtttgtca ggtaaatatt tccgtatata ttcttctaag gcttgatcca
     4201 taacttcttt tacgtttccg ccattctttg ctgtttcgat ttttatgata tggtgcaagt
     4261 cagcacgaac acgaaccgtc ttatctccca ttatatcttt ttttgcactg attggtgtat
     4321 catttcgttt tgctttttgt gcgcctaaat ttcccacaat cactcacttc tttctatttc
     4381 ttcttactct tattttatca tcaacaatca caaatcacaa gtgatttgtg attaatcact
     4441 tgtgatttgt gattctattc tttttcgttt tctaattgaa taattcgctc aagcatttca
     4501 aaaaatacgt tcttatacat ggataaaact tttttgtcat agcctttatg ctctgtaatg
     4561 ccgtttttag accaggtact tactttatta cttcgcttga taatattttg gaaaaccaag
     4621 ttatcttctt tatgttgctt atacagttct tccaggtttg attttatcgt tgcgctgtcc
     4681 gtatcaacta aataaggaac aaaaccgatc a
//
LOCUS       DACDIG010000123         4700 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.124, whole genome shotgun sequence.
ACCESSION   DACDIG010000123 DACDIG010000000
VERSION     DACDIG010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.124"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            61..915
                     /locus_tag="IV247_002745"
     CDS             61..915
                     /locus_tag="IV247_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003065868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8778746.1"
                     /translation="MENSIYLVTEPNTQLKDCYFNFCGYSKTEPRHSFGPAIREQYLI
                     HIVLEGEGYYSINDQKYFLKKGQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYY
                     LKEIGVGKNHLTFKVENLNSFKGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSAS
                     YLNEGIITKKMNPFVRQTIEIIRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISI
                     KEYISEIKISIANDLLSSTEYSIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRL
                     GLGIINGE"
     gene            1012..3261
                     /locus_tag="IV247_002746"
     CDS             1012..3261
                     /locus_tag="IV247_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP8778747.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3264..4439
                     /locus_tag="IV247_002747"
     CDS             3264..4439
                     /locus_tag="IV247_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8778748.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTRVTRGAS"
     gene            complement(4442..>4700)
                     /locus_tag="IV247_002748"
     CDS             complement(4442..>4700)
                     /locus_tag="IV247_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP8778749.1"
                     /translation="GITISVPPRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQN
                     FNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1639 a          678 c          854 g         1529 t
ORIGIN      
        1 atgtaataat ataaacataa taaaacaaaa attatccaaa tctaacatag gagggtggat
       61 atggaaaatt ctatatattt agtaacagaa cctaacactc agctgaaaga ttgttacttt
      121 aatttttgtg ggtattcaaa aacagaacct agacatagtt ttggaccagc tattcgagaa
      181 caatacttaa tacacatagt gctagaaggt gaaggctatt attcaattaa tgatcaaaaa
      241 tattttctga agaaaggcca aggatttgtt atcccaccag gagtatctac tttttatcaa
      301 gcagatgaaa aaaatccgtg gtcatatgta tggttgggtt taagtggcaa tcttgtagaa
      361 tactatctaa aagaaattgg tgttggaaag aatcatctta cctttaaagt cgaaaattta
      421 aattcattta aaggattaat ttttgaaact ttatcgtatg atcaggacaa tctagtcagt
      481 gaattgtcca gacaaaaaca agtttataaa tttttagaac tcctcttaaa ttcagctagt
      541 tatctgaacg aagggataat tactaaaaag atgaatcctt ttgttcgtca aacaattgaa
      601 ataatacgtg agaatgcgta tcaaaaatta tctgttaaag atattgctgt cagtctatcg
      661 atacacgaaa attatttatc acgattgttt aagaatgata tgggaatatc aataaaagag
      721 tatattagtg aaatcaaaat ttctattgca aacgatctgt tatcatcaac tgaatattca
      781 atccagcaaa tcagtgacaa gataggattc tcaacagtac aatctttttc taaagccttt
      841 aagaaagcaa atggtgtttc accgggagta tatcgaaaac gtagattagg tctaggaatc
      901 attaatggtg aataaactaa acatttgatt ttgataaata ttcgttactt taaacgctta
      961 ctatttatgt gagcgttttt tataatgcaa taaagaaacg gaggagagct tatgtataag
     1021 gataatgaaa tgaatattat ttatcgatca gaaaaccaac aatttcattt gtttaatcag
     1081 gagataagtt acatttttaa gatatcggaa gatggtaaat tattgcattt atattatggt
     1141 gagaacttac cagataatga ctatagtcat ctaattgaga tgcatcatcg gcctatgaca
     1201 acctatagaa atgaaaacga tttgctatat tcgttagagc acttaaaaca agaatttcct
     1261 gaatatggta caacagattt tagacatcct gctatttcat tactacagaa aaatggctct
     1321 cgcttatcag attttgtcta tcaagggtac gaagtggaag aaggaaaacc taaactagaa
     1381 ggtttacctg ctacctatgt tgataataat gatgaaagca aaacattgaa agtttatatt
     1441 ttagattcag ttacaaatgt tcaagtagaa ttattatata caatttatag agattatcct
     1501 gttattactc gtagcgcgcg agttatgaat aaaggaaaag atattcaaat aatcgagaat
     1561 attagcagtt tatcattgga tttacccgat gcaaattatg aatggcaaca attatctggt
     1621 gcttggggta gagaaagaac tattaagtcg cgtactttac aacaaggaat tcaatcaatc
     1681 gaatctactc gaggtatttc aagccatcag cataacccat ttgttacttt aaaaagaaaa
     1741 aatactgatg aaaatcaagg agaagctatt ggtgcagcac tagtttactc aggaaacttc
     1801 ttaattcaag cagaagtaga tacatgggat gttactagac tacaagttgg catcaatcca
     1861 tttggattta attggaaact caatccagga gaaatattta cagcaccaga agcgattctg
     1921 gtttattcaa gtaagggttt aaatgccatg agtcaaactt ttcatcaact atttagaaaa
     1981 cgcttagctc gtggtaagtg gcgagaaaaa gaccgtcctg tcttaattaa taattgggaa
     2041 gcaacatatt ttgattttga cgaagaaaag ttggtaaata ttgctaaaaa agcatttgat
     2101 gttggtattg aattatttgt attagatgat ggctggtttg gagaaagaga aaacgattgt
     2161 gcaggattag gagattggca tgtaaatcct aaaagactac ctagaggaat cggcagcctt
     2221 tcagaaaaaa ttcgtagttt tggtatgaaa tttggtttat ggtttgaacc tgaaatggtg
     2281 aataaggata gtgagttata tcgcacacat ccgaattgga ttatttctac acctaatcga
     2341 aaacctaatc atggtaggaa ccaatatgta ttaaattttg ggttagatga ggttgttgaa
     2401 aatattttcg atcaaatgtg taaaatcatt gatgaatcaa acttagatta cattaagtgg
     2461 gatatgaatc gtccattgac tgatgtattt gacagtcact tgtcagcaga tcaacaagga
     2521 gaagtattcc acagatatgt actaggcgta tatcgcttat atgaaaaact tataacaaaa
     2581 tatcctaaca tactatttga gtcttgttca tcaggtggtg ggcgctttga tcctggaatg
     2641 cttcactatg cacctcaagc ttgggctagt gatgattcag atgcaattga aaggttaaaa
     2701 attcaatatg gaactagtat gctgtatcct ctttcgtcta ttggtgctca tgtatcaatt
     2761 gttccaaatc atcaaacaaa ccgtataaca cctataaaaa ctagaggaaa tgtagctttt
     2821 tttggtgcat ttggatatga gcttgactta aatgaattaa gtgaaggcga tttgaggatt
     2881 gtaaaagaac aaattgcctt ttataaaaaa tatagaacca taattcataa tggtactttt
     2941 tatagactat tatcaccatt cgatgatgat aatcaaaccg cgtggatggt tgttagtcat
     3001 gataagaaaa cggctattgt tgctcattat aaaacattaa atgaggtaaa tgctccttac
     3061 cgccgactga aattaaaagg gttagataat gacggacttt ataatgtagg acaaaatagt
     3121 ttgcgatctg gaatggaatt gatgagagca ggactagtct gttcagatgc atctagtgga
     3181 caaataaatg atcagaatcg acgcccagaa acatttgatt ttgattccaa gatttgggtt
     3241 ataacacagg aggaaaagta gagatgaata accaaaaaag cttacttcaa aaaggagcaa
     3301 tcttaagtat ttcactaatg ttaacaagct ctcaagcaat taatggtgtt attccacaaa
     3361 tgaaacagtc tttggggata tcacaaagtc aaagtgaact tttaggaact gcaccaagta
     3421 taactgttat tttgtttatc ttattatcta gttactttgc caaaagaata ggaatgaaaa
     3481 agacaataat tttaggttta ttattagctg gtataggagg aattattcca gtatttacag
     3541 ctaccttcca aaccgttctt attagtcgag taatattagg tgccggttta ggagtatata
     3601 attcattggc tgtgacatat atcagctctt tatactctgg caatactaga gcaacgctat
     3661 taggtattag aaattcaatg gaagctttag gtcagacaat ccttattttt cttgctggag
     3721 ttttagtgaa cattaattgg acggcttcgt ttttagtcta tgcaattgcg tttcctgtag
     3781 ctctcttatt cgcgctaaaa gttcctgaaa ttagagatga aaatagtgac atacctgaaa
     3841 atcatgtaaa agaaaagatg aatccaatgg tttttgccct agttctattt gcaatcttac
     3901 tcgtaatgaa tagtattgct atttctgtac gatttgcatc aattgcaact gaaattaaag
     3961 gggtaaactt taatgctagt aactatttag ctctaatgcc tatcttagga atactagcag
     4021 gatttatgtt tggaccaata aataagtgga taggaaaagg aaccctatac ttgggaatta
     4081 tattctttat aatttcaaat ttgttaattg ctgtgtcaaa cggtaatatg acgttcctgc
     4141 taacgggttt atttttatca agtataacag gatcatggtg cttcccattt atctttagta
     4201 acttagataa agtcactaca aagaatacaa ttaattttgc tacttcattg atttttattg
     4261 gctgtaatat tggtaatttt atagctccta ttgctatgca attagcacaa tttctaacaa
     4321 gaagtacaaa tttgactgct ccattttttg tattcgcagg aatatttatt gtgatcctat
     4381 ttgtaacatt ttttgcgaca aataaaaaaa atactagagt aacccgtggt gctagttaat
     4441 tttaatatcc taaagaatat ctcaaagttc cttcaaaacg tcgttgtgct cgacttaata
     4501 gaacactgta agccagtaat atgctttcaa atctactctc tagcccttta acagaacgtg
     4561 agttgaaatt ttgacaacct aatttttcta aggaagaaaa aacagtctca actgtttttc
     4621 tttgttttcc aagtaagctg tggtctagtt tttctgattt atccatgttt ttacgtggcg
     4681 gaacggatat agtaatgccc
//
LOCUS       DACDIG010000124         4590 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.125, whole genome shotgun sequence.
ACCESSION   DACDIG010000124 DACDIG010000000
VERSION     DACDIG010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.125"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            148..1137
                     /locus_tag="IV247_002749"
     CDS             148..1137
                     /locus_tag="IV247_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAP8778750.1"
                     /translation="MKKKKQDLINGTFREKKKNLKKVVAFSDNRISPKMRELMTGCGS
                     YLEFIATIDKEKKKLVQAHFCKNRFCPLCAWRKARKDSMMLSIMMQAIAEEKQYQFIF
                     MTLTTPNVKAECLNDEIDLFNHALKKMFMRKRVKTSIKGYVRKLEITYNKKRNDYNPH
                     FHLILAVNKSYFTDKDYYIKQSEWLDLWREVTGKTGINPDGTDEITQLDIRKVKGFQQ
                     EKAVQEVAKYSAKDFELIESQEVFDTFYHAMKGRQLITFNGVFKEYKKKYESGDLDRY
                     KKKDENEYFWFLTASWMRKESKYAVDYREMSADERNYFSGMYVDEKSVEGENE"
     gene            1134..1553
                     /locus_tag="IV247_002750"
     CDS             1134..1553
                     /locus_tag="IV247_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778751.1"
                     /translation="MTDSTDKMTIKALADELGITKNTVRNYLVKAGYDLARYKKNGII
                     LLDKELIGVARSIYRSKGGQLTGHLPVNSTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            1562..1870
                     /locus_tag="IV247_002751"
     CDS             1562..1870
                     /locus_tag="IV247_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778752.1"
                     /translation="MDKIDLREETVNRIEVMYERLADYLNLSDEDNYSEMLERMMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDEAEKEFWLKLMSHIMQLNQISVINSKEKGM
                     "
     gene            1874..2236
                     /locus_tag="IV247_002752"
     CDS             1874..2236
                     /locus_tag="IV247_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778753.1"
                     /translation="MGMTLADIKVQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFESFADFERWLSSISDDQQHEKSVGNELRADEKSAPSERNYPNESDGIFGGIPNRK
                     SQMSESGFASMFSDVNSR"
     gene            2458..3864
                     /locus_tag="IV247_002753"
     CDS             2458..3864
                     /locus_tag="IV247_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAP8778754.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKINDWHKHIDFTKSDEN
                     IYLEQINIREKYEELFGEAVEKYNAKQKRADRKIEDYLAKVRKDKKMEPQREFIVQIG
                     TLDDFRTTRDDGSSTGISEQQAEQNRVIANKILVEYFKEFKERNPSLSVYNAVIHNDE
                     VSPHLHLNIVPVAEGYKRGVQKQPSFNKALQQQGLRADKENGRVIWNNFRNQEVESIE
                     RLMADFGWERELVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYICRQREE
                     NEREKALVASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVAALEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQHKLERSEDEIEW"
BASE COUNT         1598 a          651 c         1042 g         1299 t
ORIGIN      
        1 aaaaaaaaag attgcttttt cttccctagt attttatagt tttaattgac tagaaaaaaa
       61 ctaaactaga aaggagagca atctattttt tgttatcggc ttttcagcct tttttgtata
      121 caaaatttgt agattcagta tagcaagatg aaaaagaaaa agcaagattt gattaacgga
      181 acatttcgag aaaaaaagaa aaatttgaaa aaagtggtgg catttagtga taatcgaatt
      241 tctcccaaaa tgcgtgaatt gatgacaggc tgtggttcat atttagaatt tattgcaacg
      301 attgataagg agaaaaagaa attggtccaa gctcactttt gtaaaaatcg tttctgtccg
      361 ttatgtgctt ggcgtaaagc tcgtaaggat tcgatgatgt tgtcgattat gatgcaagct
      421 attgcggaag aaaagcagta tcaatttatt tttatgacgt tgacgacacc gaatgtaaaa
      481 gcggaatgtt tgaacgatga aattgatttg tttaatcatg cattgaaaaa gatgtttatg
      541 cgcaaacggg ttaagacttc gattaaaggc tatgtgcgaa aattggaaat cacgtacaac
      601 aaaaaacgga atgactataa cccgcatttt cacttgattt tggcagtaaa taaatcttat
      661 ttcacggata aagattatta tatcaagcaa tcggaatggt tagatttgtg gcgggaagtg
      721 accggaaaaa cgggaattaa tccggatggt accgacgaaa ttacccagtt ggatattcgc
      781 aaggtcaaag ggttccaaca agaaaaagca gtacaggaag ttgcgaaata ttcggcaaaa
      841 gattttgaac tgatcgaaag tcaggaagtg tttgacacat tctatcatgc aatgaaaggt
      901 cgtcagttga ttacgtttaa cggtgtattc aaagaataca agaaaaaata tgagtcgggt
      961 gaccttgatc gttataagaa aaaagatgag aatgaatatt tttggttttt gactgcttcg
     1021 tggatgagga aagaatcgaa atatgcggtt gattatcgtg agatgagtgc tgatgaaaga
     1081 aattattttt caggaatgta tgtcgatgaa aaatctgttg agggggaaaa tgaatgactg
     1141 attcaacgga taaaatgaca attaaggcgc ttgctgatga actaggaata accaaaaata
     1201 cagtgagaaa ttatctagtc aaagccggat atgatcttgc tcggtacaag aaaaatggaa
     1261 ttatcttgct agacaaagag ttgattggtg ttgctcggtc aatttatcgg tcaaaaggtg
     1321 gtcaattgac tggtcattta ccggtcaata gcacgggcag tgattttcct ttggaaaaag
     1381 agaatgagtt tttgcgtgat caattagctg agaaggacaa acatatttct gaattaacag
     1441 aaatgattcg tgatcaacaa aaattgaccc tgcttcagca acaaaaaaac gatcagttgt
     1501 tactagaaaa tcaagaatta aaaaatcgaa aatggtggca attctggaag tgagatgtga
     1561 catggataaa attgatttga gagaagaaac agtcaataga attgaagtga tgtatgaacg
     1621 gttagctgat tatttgaatc tttcggatga agataattat agtgagatgc ttgaacgaat
     1681 gatggatgtt ctcaactcgg ttatcaatgc tgatctagca aaatcttcag ttttgactaa
     1741 tgaatttgtt cataagcatt tagcgcaaac aactgatgaa gctgaaaaag aattttggtt
     1801 gaaattaatg agtcatatta tgcagttaaa ccaaatttcc gttattaatt ccaaagaaaa
     1861 ggggatgtag tttatgggaa tgactttagc agatattaaa gtgcaagaag aaaaattaaa
     1921 aaaagaacag gcgaaacttc gccaacagaa gaaacgattg attgagaaag aaaatgctgc
     1981 aatgggcaag gctcttcgca agcgttttgg gtttgaatct tttgctgatt ttgagcggtg
     2041 gctatcgtcg atttctgacg atcagcaaca cgaaaaatca gtgggtaatg agttgcgagc
     2101 tgatgaaaaa tcagcaccaa gcgaacggaa ttaccctaac gaatctgacg gaatattcgg
     2161 agggataccg aatagaaaaa gtcagatgag tgagagtgga tttgcgtcaa tgtttagtga
     2221 cgtaaattca cgataagcga atattttgca tgaagcaaaa taaagcgtaa aaggagttga
     2281 gagcgtgaca cgctttcggc tccttttttc gttgtcactt gtgacggcga aaaccctcgg
     2341 agagcgtttg gaattgtgac agtcatgtca caattaggaa aacgttactc tttgacaaag
     2401 agtaacagaa aggtttacac tctttttatt agcgacagcg cgaaggaggg gtttaacttg
     2461 gggtttagta tttcgttcaa aaagggaaca aagaatacaa gcatttctca taataatcgt
     2521 gagttaactg atagccaaaa aatcaatgac tggcataagc atattgattt cacaaaatcg
     2581 gatgaaaata tctatttgga acaaataaat attcgtgaaa aatatgaaga attattcggt
     2641 gaagccgtag aaaaatataa tgcaaagcag aaaagagccg atagaaaaat tgaggactat
     2701 ttagcaaagg ttcgcaagga taaaaaaatg gaaccacagc gagaatttat cgtgcaaatt
     2761 ggtactcttg atgattttcg tactactcgt gatgatggtt cttctactgg catatcggaa
     2821 caacaagcag aacaaaatcg agtgattgcc aacaaaattt tagttgagta tttcaaagaa
     2881 tttaaagaac ggaatcctag tttatccgtc tacaatgctg ttattcataa tgacgaagta
     2941 agtccgcacc tgcatttgaa tattgttcct gttgctgaag ggtataaacg gggtgttcaa
     3001 aagcaaccaa gttttaacaa agccttgcaa cagcagggtt tacgagcaga taaagagaat
     3061 ggtcgagtga tttggaataa ttttaggaat caggaagttg agtcaattga acgtttgatg
     3121 gcagattttg gttgggagcg tgagttagtc ggtactaaca aaatcaaaga tattcatgaa
     3181 tacaaagaaa tcatgagcga aatttccgaa ttgcgtcaaa cggccgtaaa agagcgtgag
     3241 agcgtttctg atgagttgag gtatatttgt aggcaaagag aagaaaacga gcgtgagaag
     3301 gctctggtgg cttctgagag agcgaagatt gaagaagaga agaaaaatat gctgaatgta
     3361 aaaaaagaga tggcggtcat tttccaaaaa gatatggttc ctttagaaag tttcaagagg
     3421 cttcctgatg gttcgtatca tttatctggg aatgattttg ttgatttgta tcaacgagct
     3481 acgagagcaa caaatgccaa gagaaatgct gataacatga gtgagcaata tttacagatg
     3541 cttgagcaaa atacagaatt aaatgagtat gtagctgcat tggaagaaga gcgtcatttg
     3601 tctggtagaa aacaattagc agatttacaa gtggaaaata aaaagcttaa agaagaaaat
     3661 cgctctttac gccagcgttt agagcgatta aaaaacgcat ttcaaaaatc gatcactcgt
     3721 ttgtctatcc gttttgggct tgcagaaaag gatatggggc tttctgagga attagaagta
     3781 ttagagcagg agaaaacctt attttatcgt caaaacgtct cacagcataa attagagcgt
     3841 tctgaagatg agatcgagtg gtagttacga tgtgcagtca acggtaccgg aggcggtttg
     3901 tgccgaggat aaggagttga cggctaaacc actagcctat taagctgaca tttgctaaaa
     3961 aatggccagt ccattttttg gccaacttag cgttagcgaa gtttgggggc gcccttttca
     4021 aagggtgcgg ttttatgttt ttggatattt gcatagctaa agagaagtgc tattattgag
     4081 ttatcaaata aataaactga aaaaagagca aaaaaataaa gtcgagcaac gctggacacg
     4141 ttctcgacct ttacggaaac taatcacact agctccgtaa ctcaaattca ttatacgaat
     4201 aatttcaggt ttcttcaaga ctagagattg ctggttatga attttttaga gttacgtgaa
     4261 ctctggaaaa ttctttccac caatctcttt tttctttgag gaggaactaa atttgagtaa
     4321 aaaaggcacg atcataaatg agtgggaaaa attaacagcc ccctctaaca ggcggttaaa
     4381 tgtaaaaact ttttacaccc ccttaaaaat gcagtggtcg attgatcgaa ttttatgtct
     4441 ttgggttttg gggttttaaa acgaaaaaaa gggggcgatt ttcgccctct tttttgatgt
     4501 tttttcttat cttgatacta taggcaacta tttctgcgaa gaaatcgggg ctatagtatg
     4561 ggtctatccc ttgtggctct aaggaagaac
//
LOCUS       DACDIG010000125         4464 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.126, whole genome shotgun sequence.
ACCESSION   DACDIG010000125 DACDIG010000000
VERSION     DACDIG010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.126"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            574..1008
                     /locus_tag="IV247_002754"
     CDS             574..1008
                     /locus_tag="IV247_002754"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778755.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
     gene            2301..3224
                     /locus_tag="IV247_002755"
     CDS             2301..3224
                     /locus_tag="IV247_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP8778756.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            3240..3779
                     /locus_tag="IV247_002756"
     CDS             3240..3779
                     /locus_tag="IV247_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP8778757.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
BASE COUNT         1664 a          668 c          758 g         1374 t
ORIGIN      
        1 aaaaaaaact gtgttggtac tgggttctat ttctctagga atattaggtt ttagccccat
       61 gctattatcc gtaattggaa aaaacaattc caacagagaa cttaaattga attcaaatta
      121 ttacaacagt cctaacgaag aattgtttgt aaaagaaacg gataaataat aaaaaagact
      181 gctcttccga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa gcaacgcgct
      241 tataaagcgc taccaaaaat ttatcatatt ttatgaaaaa taacaatcca gtaaaaaatg
      301 actggtaaaa attcacaaag aaacggaaag aagcaactgc agaaaatcaa gccttaaaaa
      361 gagcttttta cctagaccaa ttaaaaatca caaagtgagc ccctgtatcg gttgatacaa
      421 gggctacttg gcatactcga cattatgtct actaaccttt aaaataacac gttttataaa
      481 ataaaccctc ttgacaatat ttaattttct gattattcta aatatataca gaaagcgctt
      541 tcctaggatt ttatatctaa ggaggaagta acaatgaaaa ataaaattat taaaattatt
      601 acttgtctat ttttgttcgg tatgattgtt ggagcaagta ctccgttaaa tctgtttcct
      661 tttagtgcta gaggtataaa agtggaagct gcaagttcac gatataatca taatcatcgt
      721 ggatttactt gctataaaat gaactattat gttactaata aaatgtgtag agatttgaaa
      781 aaaaattata ataagctaaa gactcctgct caaattgcat cttttattcc tattggtgga
      841 gttggaacat gggttattac aaatgctttt agtagtgctg ttgatgcaat gaatgtcttt
      901 gtaagagctg caaatcaagg aaaaggtgta caattaacat ataatgctca ctttagcaat
      961 acaacatcat atcaatacaa tgattacgct cgttatgtaa taaaataatc agaaagtgaa
     1021 actatctcat gaataaaaag attttagctg taattatttc aataatagtt ttaatcacta
     1081 tgttcttcat atttaggcta attttcaata taagcttaca aaaaagtatt ctttatctaa
     1141 ttcctatagc atttatctta gcaattttta gaagtattta cggaaataaa aactctaaat
     1201 agctaaaaat aaatcatact aaattttaca aaaagtggct agcattttct agccactttt
     1261 tgtattgtgg agcgtgggtt ttgaggggga ttcaaggggg agcttgcgcc cctttgacaa
     1321 gcggttattt gcaccaaata cggaggtatt tggctagcaa atatagagct tgccaaaccc
     1381 ttttagtgag ttttgtagaa agtaccttta aaggtacttt cacattacga acgatacgtc
     1441 cgtaggacgt actgaaaggg tgggacttat gagcaaatta atgcaaaatt tggctagcaa
     1501 aaaagctaaa aaatatatgc taataatcga gcgaaataaa cactttttca agtgagcgaa
     1561 tccgccatga caaagctgaa gcagtttatt tcagctttgt catggcgtgt gccgactttt
     1621 ttaaaagtct aggcacacga gcaaatatct ttatcaagct ctaatttgcg ttttaagacg
     1681 tttgaaacgc ctaagtatat aaattatata agaataggtt taagagtgct taaatcgaat
     1741 tttagagggc atttagacaa ttttagggtt gttagggctt gccctgaccg cctgtaaggc
     1801 gttttaagaa caagataagg gatttttata ctaggactgt cttaaaatgt cttaaacgaa
     1861 cgctcagagc ttctcaaagg gttccttatg aacggctttt agcaaaggag ccggaggcga
     1921 ctgcgcagta taatgtgaga gcagagcgat cacacctttt tagcgaaccg tagggagcaa
     1981 ggtttggaat tggcagtttt gataaattcg cgaacctgtt tagagaaaaa aggagtgatt
     2041 ttttaggcgg gaaaattagc ctcccctctt tttatatctc tttttatatc tctttttata
     2101 tctcttttat gtcggctgtg tccttactct cccaagcaat tcagagtttt ttcaagtagg
     2161 ttttctacgc ttttcaagta ggttttctac gcttttcaag taggttttct acgcttttca
     2221 agtaggtttt ccgacattat tgagtatttt tataaagtat gttactttta tttcatactt
     2281 tataaaagta ggagaagctt atggcaaatg aaattgtaaa atatgaaaat agattaaatc
     2341 atattcctct gagaaaattt aatagtcgtg aaatgaactt atttttttca attgcttcac
     2401 gtgtacgtga aaaaggtgta gatgagatta ttttttcatt tcacgatttg aaaaaactaa
     2461 gcggttatac agaacgtggg gaacaattta taaaagatct tagtagcacg tatgacaaat
     2521 tattaagtat taatgcttgg aatgatgatg gaaatacttt aactaaattt gttgctttca
     2581 caaaatatag cattatacga gataaacaac ttgttataat ttcagttaat ccagaattta
     2641 aaggactttt taatcaatta aagaactgga caagatttaa tctagagcaa tttactaatt
     2701 tggatagcac atattcaaaa actatgtttc gattattaaa acaatatcga actcaaggct
     2761 ggactgaatt ttccaaagaa aaattttttg atttactaga tattcctaaa agttattgga
     2821 atagtccgtc aaatattgac aaatttatct taaggccaat taaagaagaa ctcactccct
     2881 tatttaaagg attaactatt cgtaaaaaat atggtaaagg tcgaggtaaa cctgttatcg
     2941 gttatcgttt tacttggaaa gctgaaatta accatgctga cgatttttcc aaagggaaac
     3001 aagaagattt acgtataaaa ttatttaata ttgatcacaa tggagaatta acacgagaag
     3061 aaaaatggcg cgctaaagat cgaatactaa atcttccctt aggcactcat gaagctgatt
     3121 ttaaacaaca acaacaaact gaagaaaaac aggcagaaaa acgagaactt tcaaacaaac
     3181 tcaaacaaga tttactagga aatttacaaa acttattcga ttaagcttaa ggagtattta
     3241 tggcagataa aacaatcaaa gaacttgcgg aagaattagg agttagtaaa acagcaatta
     3301 acaaaaaagt ttctgacaaa aatcgaaaac tttggtttgc aaaaaacgga aacaagtttg
     3361 tgattaacga aaccggtcaa aaagctataa aatcaatgtt tttagtcgat aacgcaaact
     3421 taaatcgaaa actagttggc gaaaaatcaa aaaccaaaaa ccataaaaac accaaccaag
     3481 tttccgaatt aaaactcctt tctttcctag aaaaacagtt aaatcgaaaa gatatacagc
     3541 tacaagaaaa agataatcaa ataaaacaaa tgcaaaaatt actcgaccag caacaacaac
     3601 tcactctaca agcgaaccaa caaattgaga acctacaaga acagctccat ttaacttata
     3661 cagaagaaac tccaacaacc gaatcaactg ccttttctga aaaaaaagca gacaatacac
     3721 aaccacttat tgaaaaaaag tggtggcaaa tttggaaatc taatagaacc aatctctaaa
     3781 attttctaga agttagttcg gatagtagag gaaaaagttg tactacctga tggggagatc
     3841 actcaaattt ttatgagaaa gaaaaaatag tttacaactg taaacaatca atgtttatag
     3901 ttgtaaacta tcaaactata tggtgactta ttgcttaaaa taggttgaaa tctcactcaa
     3961 tttttggtgg aaaaacaaaa taaattctaa tcgattttgt tgaaaaagtc ataaaaaaac
     4021 aaatgtgatt ttaacaaaat ttcaaaaaaa tcgtttgaaa tttcgtttat tttttttgag
     4081 aattttgatc atcgtagggt cattgtagaa tcattatgag atcatcgtgg gatcatcgaa
     4141 taaagtatgg gaaaggcttg cggtgcttgg tattaagggc attctccgcc tcccaggttc
     4201 ttgaccattt tctcttataa gtgggatttt taccacgttg agagaaaatg gtcaagaacc
     4261 tgggaggcgg agaaaacata ccaagcacca caagcaaaaa aagttagagt aacttccaaa
     4321 aaagaaaaat tttttagaac aacttttgaa aatttaattt tgatttatat ccgttcaatt
     4381 taaacatttg ataaactgaa ggctagtatt taaaatgtgt atacaagtgg tagcgttaaa
     4441 aaaagtggag gtataaccta tgtt
//
LOCUS       DACDIG010000126         4453 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.127, whole genome shotgun sequence.
ACCESSION   DACDIG010000126 DACDIG010000000
VERSION     DACDIG010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.127"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(150..1670)
                     /locus_tag="IV247_002757"
     CDS             complement(150..1670)
                     /locus_tag="IV247_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP8778758.1"
                     /translation="MTKMESELLVQIKQLTQLLEIQQQENTLLREQIAEMNRRLFGRK
                     KETPPVDGQIDLLDDSTFNEPEHTGQESQEPITVSSFKRRKRKGLKALSLEGLPEVPI
                     HYELKGADCLCEDCGHTLHDIGATRLREEPLYVPAHIEKQVIYQHAYHCRECEALGET
                     KIKKAPVPKPLINNSLGSSSIVTQSIWEKFVKKVPAYRQEKEWHALGFPLDRKKITNW
                     HIKVSEMVLEPIYNCLHQELSNQEVLHADETSYRVLNTHKEKTFYWLYASGKSEEKQV
                     VLYEHANSRSVDEPKSFLESFSGFLHTDGYAAYGKLPVNNVYCWAHVRRKFFEALGKQ
                     PSPKSHAKIGLDFCDKMFRIERTFNKLSAEERFVARKETLQPVMTEFFHWCESFAYLP
                     KSQLGKAVQYAVNHKDGLQIVLLDGRLELSNNRAERAIKELVIGRKNWLFSKSLKGAR
                     SNGIILSIIQTAVANGLNIRKYLNHLFTEIPNLSSMTPEALRAYLPWNQQIQEICK"
     gene            complement(1913..2098)
                     /locus_tag="IV247_002758"
     CDS             complement(1913..2098)
                     /locus_tag="IV247_002758"
                     /inference="COORDINATES: protein motif:HMM:NF017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HAP8778759.1"
                     /translation="MPMLIDATALKHIFIVCGKTDLRKGIDGLAAFVIEEYDLDVYDQ
                     ALFLFCGTKKDRFKALY"
     gene            complement(2082..2264)
                     /locus_tag="IV247_002759"
     CDS             complement(2082..2264)
                     /locus_tag="IV247_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778760.1"
                     /translation="MNKKDIVPVQFSSAAQKSQIDTKPKLACRIKSEDKEIFIYNGMD
                     KYLLYTLLKELNANAH"
     gene            2505..2723
                     /locus_tag="IV247_002760"
                     /pseudo
     CDS             2505..2723
                     /locus_tag="IV247_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            3218..3817
                     /locus_tag="IV247_002761"
     CDS             3218..3817
                     /locus_tag="IV247_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8778761.1"
                     /translation="MKTVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLADVLEISRGRVVVKDRLAMKEQKTAKHNSVYISNKLRKTILEYVQQEFPDQ
                     LLQQDFDGYLFPSRKGTNEPLNRQSLWRIIHDAGTAIGLKDIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            3881..>4453
                     /locus_tag="IV247_002762"
     CDS             3881..>4453
                     /locus_tag="IV247_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778762.1"
                     /translation="MSTLYIIGNGFDLSHGLKTRYTDFSKWVEKSHSEIFDKIDTMIN
                     ENNEVRSTADDKIGWFNFESSLHELIQSPVFEDYVEESKDYLIGYGHEDWSDSAHHDF
                     QNMVYDYVVSLEELQQLFKEWIRKEIQPTIEHKIPKYQFSSDVLFFTFNYTDVLEILY
                     KQPKENIYHIHGSYDDIIIGHSENFDNFIGD"
BASE COUNT         1421 a          803 c          792 g         1437 t
ORIGIN      
        1 attattcagc aaatatgaga atggtttttt tatttgatta gacataactt aataattcag
       61 tagttttgag cgtaagcgtg tcatagaatg gtacctaaac acaaatagcc cgatttgata
      121 cactcaaatc aggttatttg tggcttatat tatttacaga tttcttgtat ctgctgattc
      181 catggcaaat aagcgcgtag cgcttcaggt gtcatcgaag acagattggg aatctctgta
      241 aaaagatgat ttaaatattt gcggatgttt aacccattcg caacagctgt ctggataata
      301 ctcaatataa ttccatttga tctagcgcct tttaagcttt tagaaaatag ccagtttttt
      361 cgaccgatca ctaactcttt gatggcacgc tctgctcggt tatttgaaag ttccaagcgt
      421 ccatcaagca acacaatctg aagtccgtct ttatgattga ctgcgtattg aaccgccttt
      481 ccaagctgcg attttggcaa ataagcaaaa ctctcacacc aatgaaaaaa ttcagtcatc
      541 acaggctgca acgtttcctt cctcgctaca aacctttctt ctgcgctaag cttattaaag
      601 gtccgttcga ttcgaaacat cttgtcacag aagtctaatc ctattttggc atgacttttt
      661 ggggacggtt gtttacccaa tgcttcaaaa aactttctgc gaacatgcgc ccaacaataa
      721 acattgttta ccggcagctt tccataggcc gcgtaaccat cagtatgcaa gaaaccgcta
      781 aatgattcta aaaaactttt tggctcatca acggaacgcg aatttgcgtg ctcgtagagc
      841 acaacttgtt tttcttcgct tttacctgaa gcatataacc agtaaaaggt cttttcttta
      901 tgagtattca atacccgata gcttgtctca tccgcgtgta agacctcctg attgcttaat
      961 tcttgatgaa ggcaattgta gattggctct aaaaccattt cacttacctt gatatgccaa
     1021 ttagtgattt ttttacgatc aagagggaaa ccaagtgcat gccattcttt ttcttgtcga
     1081 tatgccggca cctttttaac aaatttttcc caaatactct gtgtcacaat tgaagaggaa
     1141 cctagactat tattgatcaa cggtttagga acaggcgctt ttttgatttt tgtttctcct
     1201 aatgcttcac attctctgca atgataggca tgttggtaga tgacttgttt ttctatatgg
     1261 gcaggaacat acaatggctc ttctctaagc cttgtcgcac cgatatcatg taaagtatga
     1321 ccacagtctt cacacaaaca atcggcacct tttaattcat aatgaatagg aacttcaggc
     1381 agaccttcta atgaaagtgc tttgagtccc ttgcgttttc tgcgtttaaa agaagatacc
     1441 gtaatgggtt cctggctttc ttgtccagtg tgctctggtt cattaaaagt tgagtcgtct
     1501 aataaatcga tttgcccatc tacgggtggt gtttcttttt ttcgtccaaa caagcgtcga
     1561 ttcatttcag caatttgttc tctaagaaga gtattttcct gttgctggat ttcaagcaac
     1621 tgtgttaatt gcttgatttg tacaagaagt tcagattcca tttttgtcat ttttacacca
     1681 ccattctagc gattttctta catctattat accattttat gacaacgaaa aacagacaaa
     1741 atccttgatt taacaaggtt ttcgtctaat atatcgcacc ttttttcgtt tcttttagcg
     1801 tacatttttc ttcgattgaa aggccagtca gtaagacttc ttttctggaa cgcggccatt
     1861 gaaggtaccc actttcaaat cttttgtaca ttaatacaaa tccattttga tcctagtaaa
     1921 gagccttgaa gcgatctttc ttcgtaccgc aaaataagaa aagcgcttga tcgtaaacat
     1981 caaggtcata ttcttctata acgaaagctg ctagtccatc aattcctttt cgaagatctg
     2041 tttttccaca aacgataaaa atatgtttca atgcagttgc atcaatgagc attggcattc
     2101 aactctttta acaaggtata caaaagatat ttatccattc cattataaat aaatatttcc
     2161 ttatcttccg atttgattct gcaagctagt tttggcttgg tgtcaatttg cgatttttga
     2221 gccgcacttg aaaattgaac tggtacaata tcttttttat tcattcgttt ttctccttct
     2281 atatttgata gtttaattat agaagaaaga taatcggcgt acgagatact atcctatgac
     2341 acgcttacat ttaatttaat attttgcctc aaattagcca cactttttat agaactaaga
     2401 ggaaaaatga agttttcttc ctattaatat agttgtttga aatcttaaat atagcgtttt
     2461 acgatgtaat gggaagtatg gtgaataaat caactatgtt cgtacgaagc atttactcaa
     2521 ctaatttaat cgaatctttc aataaacaaa tcaagaaata cagccaccgc aaggaacagt
     2581 tccaaaatga agagtcgatg gaacgtttct tagtctcgtc ttttgatact tacaaccaaa
     2641 aattcctagg tcgcagtcat aaaggtttcc aacaggccga aggcgagctc gaacaaatgc
     2701 tgagtcaacc gatggagaat tagaaacaat ctacagtagg gaagaaccat tgacacaaaa
     2761 ttattgacgc tcccaactaa aagtgtcaaa ataatgatga aatcatttag ttcttataaa
     2821 ttagaaaaga gaaaataaat attccatatc tactttattg gatccgtaac atgactaaat
     2881 tctgaatact ttttaaagct tatcatttgg taaaactgaa tataagttaa ttatatagac
     2941 tgaaaatttt gaattttcag aatagaataa cttaaaagta ctgacaaaat tttttaaagg
     3001 ggcaatatta atgttctgag ataatcaaat cctaccgaaa atatcaaaaa tccaatgtga
     3061 cacaatcaga ttgtgtctca ttcgagggaa tcacacaatc ccacgtggct ctaggcagaa
     3121 ttgaattttt gcttggggct ttttccttat attgattttg aatgagactt attatatgtt
     3181 ataatgcaat taagtatcat tggagagggc gaagaaaatg aagacggtag aacctattcg
     3241 agacaaaaaa aagatcgatg cgatgaaggc aatcttggct tcgggaaaat atggccaacg
     3301 aaatttggtt cttttttcga ttgggatcaa caccgcctat cgcatttctg atttgcgtca
     3361 attgaagctg gccgacgtgc tggaaatttc acgcggtcgc gtggtggtca aagatcgatt
     3421 agcgatgaag gaacaaaaaa cagctaaaca taattcggtc tatatttcca acaaattgcg
     3481 aaaaacgatt ttggagtacg ttcaacagga atttcctgat caactccttc agcaagattt
     3541 tgacggttat ttgttcccta gtcgaaaagg gacgaatgaa ccgctcaacc gccaaagtct
     3601 gtggcgaatc attcatgatg ctggcacggc aatcggattg aaagacattg gcccacattc
     3661 catgcgaaaa acgttcggtt actttctcta taaacagggg accaaaacag aaatcattca
     3721 gtccttgttg aaccattctt ctcagcgcga aacattgcgg tatattggta ttacacaaga
     3781 agacaaagat acagctgtaa aaagcttaga tttataatat aaaagttaag tttaaacatg
     3841 gatagtttta aattagtaga ttgagtgttg ggaggaaaga atgagtacat tatatattat
     3901 aggaaacggt tttgatttaa gtcatggttt aaaaactaga tacactgatt tttcaaaatg
     3961 ggttgaaaaa agtcatagtg aaatattcga taaaatagac acaatgataa atgaaaataa
     4021 tgaggtgaga agtacagctg atgataagat aggttggttt aattttgaaa gtagtttgca
     4081 tgagttaatt caaagtccag tctttgaaga ttatgtagaa gagtctaagg attatctcat
     4141 aggttatgga catgaagatt ggtcagattc tgcccatcat gattttcaaa atatggttta
     4201 tgattatgtt gtttctctag aagaattaca acaacttttc aaggaatgga tacgcaaaga
     4261 aatacagccg acaatagaac ataaaattcc aaaataccaa ttttcgtccg atgtactatt
     4321 ctttacattc aattatacag atgttttgga aattctatac aagcaaccga aagaaaacat
     4381 atatcatatt catggcagtt atgacgatat aataatcggc cactccgaga attttgacaa
     4441 ctttatagga gat
//
LOCUS       DACDIG010000127         4433 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.128, whole genome shotgun sequence.
ACCESSION   DACDIG010000127 DACDIG010000000
VERSION     DACDIG010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.128"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(543..2048)
                     /locus_tag="IV247_002763"
     CDS             complement(543..2048)
                     /locus_tag="IV247_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8778763.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDN
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="IV247_002764"
     CDS             complement(2045..2731)
                     /locus_tag="IV247_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8778764.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IV247_002765"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IV247_002765"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAP8778765.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1240 a          919 c          729 g         1545 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgcat tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat att
//
LOCUS       DACDIG010000128         4376 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.129, whole genome shotgun sequence.
ACCESSION   DACDIG010000128 DACDIG010000000
VERSION     DACDIG010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.129"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(83..1051)
                     /locus_tag="IV247_002766"
     CDS             complement(83..1051)
                     /locus_tag="IV247_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAP8778766.1"
                     /translation="MSKNGIIVNEWEKLTALSNRRLNVQNMYTPLKMQWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQYEENIAYIEILKW
                     QDGKGRIDFNPNKIGHFLASSMKNFIHDLFLEPHFSRADIACDIIDVPDEFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRLYNKKLEQETKRKIVPPEIETWWRLELQ
                     LRRGKATDWHAMVHESLNSFASPHFLPLDVKTNDKVMIFGLIANPEMWGFLERRMKYK
                     LRDILKRESQNDELTNHLRETFSESADVLKKELDTWLQGLDVTEEE"
     gene            complement(1501..2907)
                     /locus_tag="IV247_002767"
     CDS             complement(1501..2907)
                     /locus_tag="IV247_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAP8778767.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKTNDWHKHIDFTKSDEN
                     IYLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     ILDDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDE
                     ISPHLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIE
                     RLMNDYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYIYRQREE
                     NERDKALMASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDIGLSEELEVLEQEKTLFY
                     RQNVSQHKLERSEDEIEW"
     gene            complement(3129..3490)
                     /locus_tag="IV247_002768"
                     /pseudo
     CDS             complement(3129..3490)
                     /locus_tag="IV247_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3494..3802)
                     /locus_tag="IV247_002769"
     CDS             complement(3494..3802)
                     /locus_tag="IV247_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778768.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERMMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM
                     "
     gene            complement(3811..4230)
                     /locus_tag="IV247_002770"
     CDS             complement(3811..4230)
                     /locus_tag="IV247_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778769.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVI
                     MLDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(4223..4363)
                     /locus_tag="IV247_002771"
     CDS             complement(4223..4363)
                     /locus_tag="IV247_002771"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778770.1"
                     /translation="MSGKLLDVTQYELILKNNKIDSPIIIPKHAVKYVYGVAESNGED
                     ND"
BASE COUNT         1235 a          989 c          618 g         1534 t
ORIGIN      
        1 aaaaaaacaa cgacaacgtt atcacctgtt aaattcacca accaattttt cttaccaaaa
       61 tgcttttgtt tttctgccat tcttattcct cctccgttac atctaaccct tgaagccaag
      121 tgtctaattc ttttttcaaa acatctgctg attccgaaaa tgtttctcgt aaatgattcg
      181 tcaactcatc attttgactt tctcttttca aaatatctct caatttgtat ttcattctac
      241 gctctaaaaa cccccacatt tcagggttag caatcagtcc aaaaatcatc accttatcat
      301 ttgtttttac atcaagtggt agaaaatgag ggctagcaaa actatttaaa ctttcatgaa
      361 ccattgcatg ccaatctgtt gctttcccac gccttaactg caattccagt cgccaccaag
      421 tttctatttc tggtggcaca attttacgct tggtttcctg ttccaatttt ttgttgtata
      481 gccgaatctg gcgttcgctg gcacgactgc cccaataagc tgtttccagc ttcccagacc
      541 gcccataaat tggcttgaaa gaaacaggat caaccactcg atattgtgta ataaactcat
      601 caggcacatc gataatatcg caagcaatat ccgcacgtga gaaatgtggt tctagaaata
      661 aatcatgaat gaaatttttc attgaactag ctaaaaaatg accaatctta ttcgggttaa
      721 aatcaatccg ccctttgcca tcttgccatt tcaaaatctc aatataagca atattttctt
      781 cgtattggtc taataattgc cagccgttat tcccaaccgc ttttaaatag ccattgcctt
      841 cattcacccg cattaattgc tcaaaatcta aaatcaacac atcattttga gtgtgataat
      901 aaatattttc cttcagtttt ccaacgatag taattcggtc aattgaccac tgcattttta
      961 agggggtgta catattttgt acatttaacc gcctgttaga tagggctgtt aatttttccc
     1021 actcatttac gattatgcca tttttactca aattttgttc ctcctcaaag aaaaagagac
     1081 ttctggaaag aattttcaaa agttcacgta actcttaaaa attcattacc aaaaaatctc
     1141 tagtcttgaa gaaatctaaa aatattcgta taatgattaa gaagttacgg agctagtgcg
     1201 attagtttcc gtaaaggtcg agaacgttgg tagcgttgct cgactttatt tttttgctct
     1261 tttttcagtt tatttatttg ataactcaat aatagcactt ctctttagct atgcaaatat
     1321 ccaaaaacat aaaaccgcac cctttgaaaa gggcgccccc aaacttcgct aacgctaggt
     1381 tggccaaaaa atggactggc cattttttag caaatgtcag cttaaaaggc tagtggttta
     1441 gccgtcaact ccttatcctc ggcacaaacc gcctccggta ccgttgactg cacatcgtaa
     1501 ctaccactcg atctcatctt cagaacgctc taatttatgc tgtgagacgt tttgacgata
     1561 aaataaggtt ttctcctgct ctaatacttc taattcctca gaaagcccta tatccttttc
     1621 tgcaagacca aaacggatag acaaacgagt gatcgatttt tgaaatgcgt tttttaatcg
     1681 ctctaaacgc tggcgtaaag agcgattttc ttctttcagt tttttatttt ccacttgtaa
     1741 atctgctaat tgttttctac cagacaaatg acgctcttct tccaatgtag ctacatactc
     1801 atttaattct gtattttgct caagcatctg taaatattgc tcactcatgt tatcagcatt
     1861 tctcttggca tttgttgctc tcgtagctcg ttgatacaaa tcaacaaaat cattcccaga
     1921 taaatgatac gaaccatcag gaagcctctt gaaactttct aaaggaacca tatctttttg
     1981 gaaaatgacc gccatctctt tttttacatt cagcatattt ttcttctctt cttcaatctt
     2041 cgctctctca gaagccatca gagccttatc acgctcgttt tcttctcttt gcctataaat
     2101 atacctcaac tcatcagaaa cgctctcacg ctcttttaca gccgtttggc gcaattcaga
     2161 aatttcactc atgatttctt tgtattcgtg aatatcttta attttgttgg taccgacaat
     2221 atcccttttc caaccgtaat cattcattaa tcgttctata gagtcaactt cttgatttct
     2281 aaaattattg aaaagcactc gaccattatc tttatcagcg tgtatccctt gttgaaccag
     2341 tgctttatta aagctcggct gcttttgaac accccgttta taaccttcag caaccggcac
     2401 aatattcaag tgcaggtgcg gacttatttc gtcattatga ataacagcat tataaacagc
     2461 caaattaggg tttctctctt gaaactcctt gtaatactga accaaaattt tattggcaat
     2521 cactcgattt tgttcagctt gttgtttcga tatgccagta gaagaaccat ctgctcgatt
     2581 tgtctggaaa tcatctaaaa tgcctatttg cacaataaat tctcgttgag gttccaactt
     2641 tttatccttg cgcactttcg ctaaatagtc ctcgattttt ctatcggctc ttttttgttt
     2701 cgcattatat ttttctacca cttcgccaaa caattcctca tatttatctg ctagattcgt
     2761 ctgctctaaa taaatatttt catccgattt cgtgaagtca atatgcttat gccaatcatt
     2821 ggttttctgg ctatcagtta actcacgatt attatgagaa atacttgtat tcttcgttcc
     2881 ttttttaaat gaaatactaa accccaagtt aaaaccctcc ttcgcgctgt cgctaataaa
     2941 aagagtgtaa acctttctgt tactctttgt caaagagtaa cattttccta attgtgacat
     3001 ggctgtcaca attccaaatg ctctccgagg gtattcgctg tcacaagtga caacgaaaaa
     3061 aggagccgaa agcgtgtcac gctctcaact ccttttacgc tttattttgc ttcacgcaaa
     3121 ataatcgctt atcgtaaatt cacgtcatta aatattgacg taaatttact ctcactcatc
     3181 tgacttttct tattcggtat cactccgaat aagccgtcag attcatcggg ataatctcgt
     3241 tcgcttggcg ctgatttttc atcagcaagc cactcattat ccactgattt ttcgtgttgc
     3301 tgatcgtcag aaatcgacga tagccaccgc tcaaaatcag caaaagtttc aaacccaaaa
     3361 cgcttgcgaa gagccttgcc cattgcagca ttttctttct caatcaagcg ttttttctgt
     3421 tggcgaagtt tcgcttgttc ttttttaatt tttcttcttg cgctttaata tctgctaaag
     3481 tcattcccat aaactacatc tccttttctt tggaattaat aacggaaatt tggtttaact
     3541 gcataacatg actcattaat ttcaaccaaa attctttttc tgattcatca gttgtttgcg
     3601 ctaaatgctt atgaacaaat tcattagtca aaactgaaga ttttgctaga tcagcattga
     3661 taaccgagtt gagaacatcc atcattcgtt caagcatttc actataatta tcttcatccg
     3721 gcaaattcaa ataatcagct aaccgttcat acatcaattc aattctatcg actgtttctt
     3781 ctctcaaatc aattttatcc atgtcacacc tcacttccag aattgccacc attttcgatt
     3841 ttttaattct tgattttcta gtaacaactg atcgtttttt tgttgctgaa gcaaagtcaa
     3901 tttttgttgg tcacgaatca tttccgttaa ttcagaaata tgcctatctt tttccgctaa
     3961 ttgttcacgc aaaaattcat tttctttttc taacgggaaa tcattgcctg catcattgac
     4021 cggtaaatca ccagtcaatt gaccaccttt tgaccgataa attgaccgca caacaccaat
     4081 caattccttg tctaacatga taaccccatt atctttatac cgagcaaggt cataacctgc
     4141 attgaccagt tgattgcgca ctgtattttt agatataccc aactcatcag caaggttttt
     4201 tatggtcatt ttttctggca aatcagtcat tatcttctcc gttactttca gctaccccat
     4261 aaacatattt aacagcatgc ttagggataa tgattgggga atcaatttta ttatttttca
     4321 aaatcaactc atactgcgtt acatcaagta acttaccgga catttttttg ctatcc
//
LOCUS       DACDIG010000129         4335 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.130, whole genome shotgun sequence.
ACCESSION   DACDIG010000129 DACDIG010000000
VERSION     DACDIG010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.130"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            63..296
                     /locus_tag="IV247_002772"
     CDS             63..296
                     /locus_tag="IV247_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778771.1"
                     /translation="MDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVFVYLVCSYL
                     LKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(455..577)
                     /locus_tag="IV247_002773"
     CDS             complement(455..577)
                     /locus_tag="IV247_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAP8778772.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            767..991
                     /locus_tag="IV247_002774"
     CDS             767..991
                     /locus_tag="IV247_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778773.1"
                     /translation="MIKTQPLLVRFTLRTSHEPINCVVANIEIADKLLILEEGKYTIS
                     VTGHFNKRKQLVIESMQIRNPDHFTRSMSI"
     gene            1434..1640
                     /locus_tag="IV247_002775"
     CDS             1434..1640
                     /locus_tag="IV247_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP8778774.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            1640..1891
                     /locus_tag="IV247_002776"
     CDS             1640..1891
                     /locus_tag="IV247_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778775.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEVLLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENLETYENEYWLTKLEN"
     gene            2072..2344
                     /locus_tag="IV247_002777"
     CDS             2072..2344
                     /locus_tag="IV247_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778776.1"
                     /translation="MNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQ
                     ALELTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            2789..2968
                     /locus_tag="IV247_002778"
     CDS             2789..2968
                     /locus_tag="IV247_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778777.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            3027..3383
                     /locus_tag="IV247_002779"
     CDS             3027..3383
                     /locus_tag="IV247_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778778.1"
                     /translation="MLKTKKNSYKLKLSILSIICLLGMIISGHLYRVAYTQFIEYKVH
                     SLGLAGDRYVEYVHYSLKCSSYILLWLIFSLFELLNLIAISSYHLDKLNTKSSRYNEV
                     FKENVKLESDLNKLKK"
BASE COUNT         1528 a          675 c          836 g         1296 t
ORIGIN      
        1 gatgatctac cgttttagtt tgagaaagaa ggagtaagca tggcaaaaaa attagtctga
       61 cgatggatct attgataccc gtaattgcat ttatagacgg gatttctttg ggcgtcactg
      121 aaaatccttt ggtaaataga attgcgaaag gatcactatt tcttgtattc gtgtatcttg
      181 tctgttctta tcttttaaaa agagcaggaa aggataattc cttttggcga ttgactaaag
      241 tcacagtagc agcgacagct gttatactta cggctattct accgctttat atgtagagtt
      301 aacgggaaat cgttgaactt cttgcgaaat attgctataa tgaacatgaa aaagaggagt
      361 tgcgctaaca acccctcatt aacagcaccg attaaagaac ggtggcagaa tattagttat
      421 ttggaaataa ccgctgacct gctaaagtag acggttattt ctttttgtca tttttaagca
      481 tatttacaat caaactaatc aaagcaatgg taaacatacc aaaaccaaga atggtttgaa
      541 ttgtctcata tgcggacaaa aggcttactc ctttcataga ttttagcact tacattcata
      601 agcaccacca cctttccaga agtcgccacc gtcttcaaac tttactgtct tgactattat
      661 agcatatcta aaatcgaata aaaattctca attttagctg tttttgctat aattttagcg
      721 aggtgatcgt gatggaacaa tatattggtt tactcgataa attagggtga tcaagacaca
      781 gccattgctt gtgcgattta cattacgcac gagtcatgaa ccaatcaatt gtgtagtggc
      841 caatatagaa atagcggata aattgctgat tttggaagaa ggcaagtata ctatttcagt
      901 gacaggtcac ttcaataaac gaaagcaact agtcattgaa tcaatgcaaa ttagaaaccc
      961 tgatcatttt acgcgatcga tgagcatata aaagaagagc tgcagtccta aatgaggatt
     1021 gtagctcttt ttgttttaca tagaaacagt aaaaatgaaa aggcctgttt aggaaggcac
     1081 aagcgccttc tcaattgaaa gacctttctc tcactccaac cgccaccgcc gttcgagaag
     1141 aaaggaagtg aacgctacgc gttcactaag ataaagggtc ttgcatagca agacaaaata
     1201 gaaaaaattt cctctcacca aaaggttcgc tcctgttcgt tatctcttta ttgatttgtc
     1261 agaccggcaa caagttgcct tttatcacca aaaagcgaca aggacaaggg taaatggcac
     1321 gctgcgcgcc cttgtcttgt cgcttttcgt tgattcattc tggccaacaa atcaataaag
     1381 aaagaactaa cgaaaggaag taaaaccttt cctaagagga ggcagaatca atcatgcgaa
     1441 agaaatttga ttattggggt gttcccttca gcgaattatt cccaaactat catgcaccac
     1501 atactgtcga atgcgattgt ggagaaagag cgaaatgtat caaaagctat tgtttgtatc
     1561 aatgtccaac atgcgggaaa aaatatacct tgtcttatgg cgattatatt ttaattgatg
     1621 agaaaggaaa gaaacgctga tgaaaacaca acacaaatta ttgactgtaa aagaagtcgt
     1681 tcaaattttg gtggacaaag aagtcatttc tgaaaaagta gcagaagttt tacttattcc
     1741 gcggtactgg acgatagaaa cccctttagt agaggatgca ccgactatgc tatttgaaga
     1801 cctagtgaag attgcaggat atgaactgca ttatcaagaa aacttggaaa cttatgaaaa
     1861 cgaatattgg ttaaccaaat tagagaacta aaaaggagaa aaaaatatgg ctcaaaatta
     1921 ctatgacgaa tttgtcaaat tacctttgga taaaatggca caaaaaatgg aggatatgac
     1981 gttcctatac aacgaaacac gtgttccaaa aaaacattat aaagaaaaat tgtcggtagc
     2041 tgtggaagaa atgattgaat aaggtgtcga aatgaatctt attgcgacgt actatcgaac
     2101 actggaagaa ctgaaaaagc aaaatgcaaa atggtttttc caagccttac tatgcttgga
     2161 agtaggtgtc aaaccaagca ctattaaacc ttctgaatac caagctttag aactaacgta
     2221 tgcgaaattt atcgaaacga aaaaagccaa gactgtttca agtgaatggt tagattattt
     2281 tgaaaatatc aataagtatg gcgcatatta caccatgaaa aaggaagaca acgaaaatga
     2341 ataaggtgat ttatcaacta acaacggatt ccatatcaac gatttgaaag aaaaaaaggt
     2401 caataatcat ttataccgaa aagtgagaat gtgtgaaaca aggacaaaaa aagcctagta
     2461 actaaggaaa tggttcttat ttcgcaccaa tcgctccaac cgcccccgcc gcttaggtgg
     2521 tgcgaaataa acgggcaaag cccgataact taagagaaag aaggtgatcc atttgaacaa
     2581 gattattccc ttattgttag tactgtttgt tggcgaaata cttatgcgag atagtgcagc
     2641 tgtacagtta aacctcgctg tatttttggt aatactggtg ttttgtttga tcttttggcg
     2701 gtttgtatta gtcctcgtga gtctactatt caagatgatt gcagccatat ttttcatagg
     2761 gttatttcga tgaaaggagt gagtaccaat gccaattgca ataggaaata aacggttacc
     2821 cgtgacatta gatgaaaaga gacaaaaaga attgcagcaa ctaaagcaga agtatggcaa
     2881 aagtgaatct aagattatgt gtattgcgtt agatctattg atcgcgcaag aaaaagcagg
     2941 atttgatgta ccagcgctaa aaaagtgacg tcacttttta atttgatcta tataagtgat
     3001 atcactttta ccaaggaggg aattaaatgt taaaaactaa aaagaattcg tacaaattaa
     3061 agcttagtat actatctatt atttgtcttt taggcatgat aattagtggt catttgtata
     3121 gagtggcata tacgcagttt attgagtaca aggttcactc tttaggatta gctggggatc
     3181 gatacgttga atatgtccat tattcattaa aatgttcttc atatattctt ctttggctta
     3241 ttttttcatt gtttgaattg ctaaatttaa tagcaatatc ttcatatcac ttagataagc
     3301 taaataccaa atctagccgt tataacgaag tgtttaagga aaatgtgaag ttagaaagtg
     3361 acttaaacaa gctaaaaaaa taaaaagaag aaaacataag ttaactaaaa ttattttatt
     3421 aagaatgatg gaatataagg ataaggttac caacataacg atcattactt ctctgatatg
     3481 ctatggactt ctatcaatca gtattttgtc tatagattat ggagtaacta tactgcaagc
     3541 cttagttttg gctatgccta tgtatctatc tttgtttatc atgcatgaaa tatataagct
     3601 atttgtttta tcaaaaaatg tagaaatacg ggtaaatatt ttggtgaata gttatcaaaa
     3661 agaactcaaa gaaagaagac ggtggcggta tttattgaaa ggatgaaaaa gtaggatttg
     3721 acatactggt attcagaaag tgccgtcact tttaaaacaa tggaaaaggt ggagataaga
     3781 tgaagaaaaa acaaactaaa ctatcattag cagatattta acacagttaa ccgcaactga
     3841 agatggtgtt gttgaatttg aacaatctga aattgcggta gtagatgatc gttaccttaa
     3901 aatgccttac ttctttgacc aagcaaaggt catttgcctt tgtcactacg atggggtacg
     3961 tgattttagg cgttaaaaag tagtgattat atcaacttta cgagtaaata taaatttaga
     4021 catgaaactg aattatccaa tggaaaatca tggtttataa tctagaaaaa ttatagtaat
     4081 gattggatta tatatattct ttcgtatttt cgtacaactg tagcttgtca aaattgagaa
     4141 atttaaccga ttaggtactt tacatcgtta tttgggtaat ttctaaaaca tgaagcttac
     4201 caacgaataa actgagacta tcttattaat tgatcaatat aagaaaatat ctttgaggag
     4261 atgatgtact atgttttcgt ctatccacga ttggttaggt ggtcttttcg gcggtggagg
     4321 taaaggtttt taata
//
LOCUS       DACDIG010000130         4248 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.131, whole genome shotgun sequence.
ACCESSION   DACDIG010000130 DACDIG010000000
VERSION     DACDIG010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.131"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..311)
                     /locus_tag="IV247_002780"
     CDS             complement(<1..311)
                     /locus_tag="IV247_002780"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734812.1"
                     /note="malic enzyme; oxaloacetate-decarboxylating;
                     NAD-dependent; catalyzes the formation of pyruvate form
                     malate; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAP8778779.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     N"
     gene            complement(752..2071)
                     /locus_tag="IV247_002781"
     CDS             complement(752..2071)
                     /locus_tag="IV247_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HAP8778780.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            2328..2798
                     /locus_tag="IV247_002782"
     CDS             2328..2798
                     /locus_tag="IV247_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAP8778781.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            3020..4123
                     /locus_tag="IV247_002783"
     CDS             3020..4123
                     /locus_tag="IV247_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP8778782.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT         1375 a          711 c          892 g         1270 t
ORIGIN      
        1 tcgttgtatt gttcgattga atccgcaacg acaggatcat aaacgacagg catgaattca
       61 actaaatgtt cgcccatcaa tttataaaac aatgtccggt tcgtattgaa tagattcatt
      121 aagaagatac gtttttccaa atcggtctgt ttgcttaagt attgaccgta agcttggaca
      181 gattgttgtt ccaatgtttg gacagtactt ggaagcatac cagtgattcc gtattttgcc
      241 cgttcttctt ttgtaaaagc tgttccttta tttagaaaag gattatttaa taagtttagc
      301 cctgtaagca tgaatgattc ctcctaagtt ttaatacaaa cgaagtatag gagtcagatg
      361 aggatatagt caaaaagagc aggtataata aattttattt atatgagtaa tagagtcaaa
      421 agaaatcaaa atagcggtat ttgttctttt tatatcaaca atccttgctt gattactatg
      481 cgtatctctt atatgctaaa gaaagaaaag tagaagaata aaggatatgt tagatagata
      541 aatagaatcc tgaataattc atagctttaa ttctttggca cattttattg aaatatgtat
      601 cactaatatc cctatcaact gattttgact aaaaagttac ttcaaacatt cactgttcaa
      661 aaatattgag gaataaattt tgggtgtaag gcattttgag catacttctc cctggataac
      721 ttcccagtaa aaaaatcgtt agattgttgg attagatcca ttgactggct cgcctcaggc
      781 gagcaaagac cctgtagaat tcattctgat acacatgata actagataat ttgagataga
      841 ctcgtctacc cgtttggact aatttcccag caactttcag aaacttcaat cgaatagaat
      901 gaatggtcat tcccttttgg acttcttcaa agccaatcgt tcttaagaag ttgactaagt
      961 tgtaagctat caagctgagc atcattcgga catgattctc caaaaagcga ggactgtctg
     1021 ttttgtcaaa gtaaaagcca gctttcgctt ctttaatgaa gttctccatt gtgccacgtt
     1081 tggcatagat agaaaagatg gtatcaggag aaacattttc tgatagattc gtcacgataa
     1141 attcatgtcg aaagagtagt tcgcccgctt cacgtgttga gcgtatacac acgcgacgac
     1201 tttgtgacca ggattgtgct tgataagtgg tcgagaagta ctgaacttct cgttcttccc
     1261 acttttgatt atcgccataa agtactgatt tctcagctat ttgacctagt ctacgattat
     1321 tcttcagtcg aataacataa tgactctttt ttgattcaca cgaatcatac acaccaggtg
     1381 tagcgaaccc gctgtctcca cgaaccaaga tgtcagtgtt tggtagagag tgattatagt
     1441 gctctaataa aggtgtgaga aactccttta cgccttttga tgtatattga tttcctgaac
     1501 gtagttcagc ttttaagaag tcaccagtca atccgtcaaa agctaccaat ggatggtaac
     1561 cataggtttg gtagtgtgta ttataattcg tttgttcttg gtgaccaaat gtatctgaat
     1621 gggtcgaatc taaatcaatg attaattccg tatcattacg gatgaggcgt gctttatcga
     1681 taagttcttg gttcaaagct tgaagttcat ggatattctc ctcagatagt cgatctaaaa
     1741 atcgggaaag tgaagattga gaagcgagtt ctttcctatc taaaacagct ttaaacacag
     1801 gatcttgtct caggagatta gctgcagaat cagctgagta accagcaatt aattgcataa
     1861 tgaactgctc aagtatggat aagttgtcat gagtaaaata cgctcgttcg tcttcaatgt
     1921 gaatgtgttg cttagccagt tcagaaaaac caaagatgtt catcagctct ttcactagga
     1981 cgagacccga atcactcgat aattgaccac ctgtatgaga aatagtgata tttgaattga
     2041 attttacttg gtttttgtgt aagctagtca tgaaaagaac tcctttcttt tggttggttt
     2101 agtcacttta accatagcag aaaggggttc ttttttcatc acttaacggg tgaagatgaa
     2161 aaagtaattg taagctgccg tgaggcagtt tcacatggtt ttaattaaaa gtatgaatta
     2221 ttcaggtaga atctctgaag aataaatcac gtgatcgtga cgataaaata cacttacgtc
     2281 aggtgataaa aaatacataa gtagaaaact aagaggagga acagaaaatg aaagaagttc
     2341 aattgatcag aaaaagcgaa ctatcagaag gtggttgcaa tgcatgcggc gtagtagaag
     2401 caacaagcta tacattaaag cttggtgcca acaaagcgat aatctctgag ttgacagtag
     2461 gcggtcttgt ggacagtctg gcgcttgctg aaggttttat tggagaagat atttatgaga
     2521 tgttttcaga aatccgtcag ttaaaaaaag gggaaaactg catagaggtt catcatgaga
     2581 gcccgaatgt gagattcaaa cgtggtgata atgagatgat cttcaataat catgtgtccg
     2641 atcacaccga gctatatgga atcgtgaatc aaatactaac tgagcttttt ggattgggac
     2701 cttatgcgtt caaggaagaa aacggtaatc caaaattaaa cgaagaatgg caagaaacga
     2761 ttgaaataca aagaaacaac ccgcacttat ttcaataggc atccacagta tttcgtaaaa
     2821 gggactttga cacgactttt gtcaaaatcc catcgttttt tatctatttg tgttctaaac
     2881 atttctttca gagtagtaac aactggaatg actgaaatgt aaacagatta tttgcctgta
     2941 tcaatacgga agagtaatat aaagtgtgta aacgctatca tattattcat tcaatttttt
     3001 atagagggaa ggcattgaaa tggaaaaaac gtatcaagca ggtacaaatg aaggaatagt
     3061 ggattttatc aatatggaag atttagaaat agccgcttca caagtgattc cagctggtgg
     3121 ttatggttac atcagcagtg gtgctggcga cttatttact tatcaagaaa atgaacgggc
     3181 attcaatcat cagctgatta ttcctcatgt attaagagat gtagaattac ccgatacaac
     3241 tacacatttt gacaaagaaa cgctgactgc accaatcatc atggcaccag ttgctgctca
     3301 tggcttagct catgtgaagg cggagaaagc ttctgcaaaa ggtgtagcag attttgggac
     3361 gatctataca gcaagttctt acgcttcttg tacattagaa gagataagag aagcaggcgg
     3421 ggagaaagca ccgcaatggt ttcaatttta tatgagcaaa gatgatggga tcaatttgga
     3481 tattcttgaa gtggccaagc gtaatggagc aaaagcaatc gtattgacag cggatgcaac
     3541 agttggcgga aaccgtgaaa cagaccgcag aaatgggttt accttcccac tgccaatgcc
     3601 gatcgttcag gcttatcaat caggagtagg ccaaacgatg gatgctgtgt acaaatcttc
     3661 caaacagaag cttagtccaa aagatgtaga atttattgct gctcattctg atttgccagt
     3721 ctatgtgaaa ggtgtccagt cagaagaaga tgtctatcgt tcattagaat ctggtgctgg
     3781 cggaatctgg gtatctaatc atggtggccg tcaactagat ggtggtccag cagcgtttga
     3841 ttcgttgcaa tatgtggcag aagcagtaga taaacgtgta ccaatcgtgt ttgacagcgg
     3901 tgtacgacgc ggacagcatg ttttcaaagc aatcgcatca ggtgcagatc ttgttgctat
     3961 tggcagaccc gttatttatg gattgtcttt aggaggaagt acaggagtcc atcaagtgtt
     4021 tgatttcttt aaaacagaat tggaaatggt catgcaatta gctggaacac aaacagtaga
     4081 agatatcaaa aaaataaaat tgcgtgagaa tcgttttatt taagaaacaa atgacgaaaa
     4141 aaataggagt gcggcagatg atgccgtact cctattttta tagttcaagg atgtcactaa
     4201 tagaataatt tgaaacgaaa aaataaaatt gaaatatttt ttcatact
//
LOCUS       DACDIG010000131         4239 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.132, whole genome shotgun sequence.
ACCESSION   DACDIG010000131 DACDIG010000000
VERSION     DACDIG010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.132"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..379
                     /locus_tag="IV247_002784"
     CDS             <1..379
                     /locus_tag="IV247_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778783.1"
                     /translation="GVIKVHKVDDQGKALANAEFTLTDDKGVKVVQKTDSNGLATFNI
                     VANKTYKIEETQNPNGYTGKWSKEGITVADDEQTFEYTVINHLVPETVRTGGIGTSAF
                     LLVFLIAGLSMVLLYYREQHKAK"
     gene            428..2263
                     /locus_tag="IV247_002785"
     CDS             428..2263
                     /locus_tag="IV247_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP8778784.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPIQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANKTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     NYEKYVVKDVLGAELTYDSSEVIEGMTLDTDYSIERDGQTTNYVFTESGMDKLQKQFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNKVTYLKQDPTTKAVTWIADKSKATEFVSGSDGKFQ
                     IEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSE
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            2338..3195
                     /locus_tag="IV247_002786"
     CDS             2338..3195
                     /locus_tag="IV247_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8778785.1"
                     /translation="MKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNSYK
                     EKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNGNLQKDKLGELVG
                     EILIPKINVDLPIYDGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKYFT
                     DLELLKLGDKFFIDISGKKLAYQVDRIKIILPTEFEDLLPVAGKDYVTLMTCTPVGDN
                     SHRLLVRGVSIPYQKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKIVA
                     KRRGMKNEE"
     gene            3185..>4239
                     /locus_tag="IV247_002787"
     CDS             3185..>4239
                     /locus_tag="IV247_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8778786.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDGGSFDIGEAEGKVNPYTGDVFSKNQT
                     KDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLPLSGKGHQTVI
                     AGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQNSTLTDIPDL
                     DMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDSEYVSDKTETV
                     TSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTGITNKHQKMLL
                     VIWTVIGALASLNVLIL"
BASE COUNT         1570 a          654 c          847 g         1168 t
ORIGIN      
        1 aggtgtgatc aaagttcata aagtggatga tcaagggaaa gcattagcaa acgcagaatt
       61 cactttgaca gacgacaaag gtgtaaaagt tgtacaaaaa acggacagca atggcttggc
      121 aacatttaat attgttgcca acaaaacgta caaaatcgaa gaaacacaaa accctaatgg
      181 ttatacaggg aaatggtcta aagaaggcat caccgttgct gacgatgaac aaacctttga
      241 atatacagta attaatcacc ttgtacctga aacagtaaga acaggcggaa tcggtacatc
      301 agccttcctt cttgtatttc taattgcagg attgagtatg gtgctactgt attatcgaga
      361 acagcacaaa gcaaaataaa aaagtgttaa tggaaaagaa gtaaatatga aaaaagaagg
      421 ctgacgtatg aaatcaaaaa aaattttttc aaatttaatg ctatttggta ctttgctagg
      481 agtgggattg ggcgcaagct caatctcttc tagccttgga ctaggggggc aagtagcata
      541 tgcacaacaa aactataatt atcaaggaac tggaacgatt catatccata aagtaacaaa
      601 tactaatggg gatgggaacc ctgtctcaga tattgataac aaagggaaag aaatagcttt
      661 accaaatggt gtaaaacctc ttgaaggcgt tgagttctct tatgtgaaac ttactaaaga
      721 tgaagctaaa tccttgggat ggacggatag tgatactaca aaaggtcaac atattgatga
      781 tgctaaagca cgattattac taaacaaaaa atacagtgaa gtaagtagaa tgaagactga
      841 ttcacaaggg aatatagaca tacctataca agtaaataca gacgatttaa cagataactt
      901 gtatttgatt gttgaaacag gaacagtcaa aggtgtagtt gaagaagcgt atcctatgat
      961 tgttaacgta cctgaactaa acggatcaga tgatcaatac tttaattatg atgtacatgt
     1021 ctatcctaaa aactatttcc aattaaactc agcagagctt gataaaaaag acgaagatgg
     1081 aaaggctctt gccggaactg tttttgaatt gcgaaaagat gatggatctg atcaggttgt
     1141 tggaacttat aaaacagaca gcacaggtaa attaactgta gatggtttgg aattaggtga
     1201 ctattatttc ctagaaaccg acttgggaac agatgctaat aaaacaacct atttactaga
     1261 tggtaaaaaa ttcaaattta gcgttaaacg tggggagact gctaaagtag ttcttgatgt
     1321 taccaactac aaacgacctg taattactaa ggtaattgat aaagaaaccg ttgatactgg
     1381 cgaagatgtt caatatacat tgaaaccatc aattcctggt aatattggta attacgaaaa
     1441 atatgttgtc aaagatgttc taggtgcaga gttaacttat gattcatcag aagtgatcga
     1501 aggaatgact ttagatactg attacagtat cgaacgtgac ggacaaacga caaactatgt
     1561 ctttactgaa tcaggtatgg ataagcttca gaaacaattt gatgcagggg atcgcttctt
     1621 tacaattttg atgaacgctc atactaatag taaagctaaa atggatacac cacttaagaa
     1681 tgatgtttca acgatctaca acaatggata tgaacccgaa gacgaaacac cagttgatca
     1741 cgcttataca atcattggtg atcaccaatt cattaaaaca gatggagata caagcaaagc
     1801 attgagtgga gcaaaatttg tagtgaagaa caaaattgat ccgaataaag ttacttacct
     1861 aaaacaagat ccaacaacga aagctgttac ttggattgca gataaatcta aagctactga
     1921 attcgtttcc ggatcagacg gtaagttcca aattgaaggc ttgcaatata cctatgatgt
     1981 agattttgat aaacaaggta atccaacaat cacagataaa aaaatcaatg attatgctct
     2041 tgaagaagtt gttgctcctt caggatatac accaactaga aatgatgttc catttgtggt
     2101 atcgaatgat gatgcttcta gcgaagtaac agacatcaag aaccttaaaa cacctgaaac
     2161 agttcgtaca ggtggaattg gaacagtagt atttctagtg gttgcaggtg tggcaggaac
     2221 agggctatat gttgtctaca aacgagaaca acacaaagct taatgaaata aaattcccta
     2281 gagtgttttt ggctctaggg tttcttttta aaaagtgtgt gaaaagtagg tatgagcatg
     2341 aaaaagaaga atatcaagtt gatcatctta aaaattggga tgttgctatg tttaggagta
     2401 ggtctatatc ctgtaattac tcaggcttat acctactacc aaactcaaca aacactaaac
     2461 tcctataagg aaaaatatga tgaagcttct gaaaaggaga atgaagaagt cttcaagaga
     2521 tattcagaag ttaataggaa acgaaaagac acagaacaaa cctcagtcac gattcctgaa
     2581 gataataaca atattccgtc aaatggaaat cttcagaagg ataaattagg tgaattagtg
     2641 ggtgaaattc ttataccaaa gattaatgtg gatttgccaa tttatgatgg tgtaaacgat
     2701 tttcaactat cagcaggagc aggaatatta accggaactg attatccaac tggtgggatt
     2761 ggaaaccatt ctgttatatc agctcattcg ggcattccta ataaaaaata ttttacagat
     2821 ttagagttgc tcaaacttgg tgataaattt tttattgata tttcagggaa aaagcttgct
     2881 tatcaagtag atcggataaa aataatccta ccaaccgaat ttgaggattt gcttcctgtg
     2941 gcaggaaaag attatgtaac acttatgaca tgtacacctg ttggagataa ttctcatagg
     3001 ttactcgtta gaggggttag cataccttac caaaaaagta ttgttgagaa agaaaagaag
     3061 aaagaagatt taaaggtaaa ggcgcagatg aactggtatc ctatcgtagt tgtaggggtt
     3121 ttattaataa tagctgtgat agtcattatt aaaaaaatag tagcaaagag acgaggaatg
     3181 aaaaatgaag aataaaatac tacttttttt gactcttatt tgcactttgg ttttaacaat
     3241 ttatcctgca aatcagctct atcatcaatt caaaacaaaa gagaatgtgg cggaaatcca
     3301 agagaaaatt gagaaaaaac aaactccttc aaaagaggaa gtggcaaaaa aagtcgaaca
     3361 aataaaatcg aacagcggta accaaggatt agacaatact cagaaaacag acggcggatc
     3421 atttgatatt ggtgaagctg aaggaaaggt caatccttac acaggagatg ttttttcaaa
     3481 aaatcaaacg aaagattttg aaccatctac ctattcaggg gcggttggtt atgtctatat
     3541 tccaaaaata aatgtgctag agcttttgtt tctaggcgct tcacaggaac atttatatag
     3601 aggtgtggca cagatgagtg ctactagtct acctttatca ggtaaaggtc atcaaacagt
     3661 aatagcaggt catcgtggcg gttatacgtc accacaattt ctttatgttg atcaattagt
     3721 agccggagac cacatttata tagattatct tggtgaaaaa gcagtttata cggttttccg
     3781 aacagacgtt attagaaatt acggtcagaa ttcaacactg actgatattc ctgatttaga
     3841 tatgttaaca ctttatactt gtcatccata tcctcagaac tacgatcgtt tattagttag
     3901 aagtctaaga gatgaaaaag caaaatacat cacgccacaa gtaacagttg gcaataccgc
     3961 atacgctgat gccattgata gcgagtacgt ttcagataag acagaaactg ttacatcttc
     4021 taatgattta tttgaggatg ttgatcgtgc caacaaggaa ttagatggaa ataatgatct
     4081 taatattccg gaacaaggta gcgtagagat gcaagacaga acatttgctg aaaaggcaag
     4141 ttcaatattc acaggaatta ccaataaaca tcagaaaatg cttttagtca tttggactgt
     4201 aataggagca ttagcaagtc taaatgttct tattctttt
//
LOCUS       DACDIG010000132         4157 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.133, whole genome shotgun sequence.
ACCESSION   DACDIG010000132 DACDIG010000000
VERSION     DACDIG010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.133"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            179..775
                     /locus_tag="IV247_002788"
     CDS             179..775
                     /locus_tag="IV247_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP8778787.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            899..2698
                     /locus_tag="IV247_002789"
     CDS             899..2698
                     /locus_tag="IV247_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP8778788.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2976..3188)
                     /locus_tag="IV247_002790"
     CDS             complement(2976..3188)
                     /locus_tag="IV247_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778789.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(4052..>4157)
                     /locus_tag="IV247_002791"
     CDS             complement(4052..>4157)
                     /locus_tag="IV247_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8778790.1"
                     /translation="ASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
BASE COUNT         1228 a          707 c          729 g         1493 t
ORIGIN      
        1 tttaaaaaaa acaagttatt aaataagtct ttagttggat gtttttttga gatcaatctt
       61 ttatacttat gatgaactta caaaattgtt tgttttctgt catatcaaaa aatggcgcag
      121 ttagttgctt ttctagtaaa gtatagttaa caaagaacct ctaaggagtt gatgaataat
      181 gaatattgct gcctctcgaa aactgataaa tttcatatcc attatcggat taggcctatc
      241 aatcgcgctg acgatttatt ttattaattt aggtgttttc aaagatttga atgcgttaag
      301 aggattagta ggtgattcca tcattttagg accgattatt tttgtcttca tccaaatcct
      361 gcaagtagtt attccaatca ttcctggagg gatcagtact gcagctggtg tcctgatatt
      421 tggcccttat gctggattta tctataacta tattggaatc tgcattggtt cgattatcat
      481 ttttctgcta gggcgtcgtt atggaaagcc attcatactt tccatgatta gcgataagac
      541 atataacaaa tatattggct ggctggataa tcaaaaccgt tttgagaagc tgtttgctct
      601 agctattttt cttccagttg ctcctgacga tgcgttatgt ttgatggctg gattgacaaa
      661 catgtcagtg aaacgatata cactaatcat tttgattgct aaaccactgt cgattttctt
      721 atatagtatg gccttgattt atggaggtca atatctaagt ggtcttcttg gataatattt
      781 attttgtaaa attagtcatt gattgacaat gttaaatctg ggaaagggtc tgcaaaacac
      841 actgcaccct ttcctttttc attagatttg cgctacaatg tgaagagcag gtgataggat
      901 gaattatttg aaacgtagct taggtaatgc ttgggacttt ttaaaaggaa cacaagctta
      961 tttccgtgat gtcttgctga tgcatggctt tattttattt atttgtttac ccttattgag
     1021 cagtacgacc cgttttattt tacaccgagg ggcaattgac tatttgtctt atgacaatat
     1081 tccttcaatt ttttctcaac atcctggggt tctactctcg ttaatcgtag ttttgatgac
     1141 gattttactg ttagtttatt tcgaatttac ttttcttttg atgagtgttt tttttattaa
     1201 aaagaaagaa ccaatctctt tgaagcaact cttacacttg acgatacttc agctgaagaa
     1261 agtgagaccc attacttttt tgtttttcct tgcttatttt ttattgattc taccgatcag
     1321 cggattaagt tttaattctg atctactgtc aaaaataaaa atccccgctt tcattatgga
     1381 ttttattttt gctaatcgtt ggatcatcgt cagttccttt ttactcgttt atattttcct
     1441 tggctatatt gggattcgac tgatttttgc tttaccggaa atgattctac gggatcgtcc
     1501 ttttcgagca gccattcggg aaagctggtc attgaccaaa tcgcgtcttt tggccatcac
     1561 gggccaattt attgtgattg gcggtacgat tttactcctt tcttctttag gatatatcgt
     1621 tgttattttg gcacaaagta tggttgaaca atttttccca gactatgcat tgatcagcgc
     1681 tgtgtttgct atgacgcttt tacaagggat tttattattc aatatcgtga tgtctaccgt
     1741 agggattttt tatatcattg ttgatttcat ggatgacgaa ggcttcttgc ctgagatccc
     1801 taagtggttt atcccacaag cccccaactt gcgtttctct gcgctgaaaa acactggttt
     1861 gactcttttt gcagtttttt tcggtattgg cgtctgtctt tacaatatgg attatctgac
     1921 ttcagctgtt cagacaaaac cagtgactgt ctctcacaga ggtgtgagta cccaaaatgg
     1981 ggcgcaaaat actttagaag cacttgaaaa aacaagcaga gactatcatc ctgattatgt
     2041 agagatggat gttcaggaaa caaaagatgg tcatttcgta gtgatgcatg atgcaaatct
     2101 tcgtcactta acaggggtaa atggaacacc acaggatctt acattgaaag aattgacaaa
     2161 tcttacagtt actgaaaacg gagtgcaagc ccctgtttgt tcttttgatt cttatttgga
     2221 aaaagcacat aagctaaacc aaaaattact tattgagatc aagacctcta aaaaagattc
     2281 ggatgacctt gtcgaacgat ttgtccataa ataccgcaaa gttatcttag agaaccatca
     2341 tattttgcaa agtttgacgt accaaaccgt ttcagattta aaagaagcag aaccaaactt
     2401 ttatgtgggc tacatcttac ctttcaatat tgttgggccc cctgtgacgc ctgctgattt
     2461 tctgacaatg gaatatagta cgatcaaccg taacttcatt aattctgccc accaagacgg
     2521 aaaagaagta tatgtatgga caatcaacga ttcagatgct atgagtcgca tgatgtttta
     2581 tggtgtggat ggtatcataa ccgatgagat gggactacta aatgaaagtg tgaaacaaac
     2641 agatgaagaa atcacttatt cagataaact gctaaatttt gttttgggag tcggctaatc
     2701 aaaaatcgtt aatgaaaaaa gtctttatac aacaaaaaac gcataacctg ccaccgcagg
     2761 tatatgcgtt ttttgtttga cgctcataat gtgtgcttaa ctttctcttg attccttcat
     2821 cgcacgaaca atagctaaaa tagcaggaat tgcagagatc actgcaagaa taattcccat
     2881 tttccctgaa ctttttcttt ttggcatatc ttctcgctcc cttctgtctt tatttatatt
     2941 ataagggagt tcttgcgaat tctcaaaaat actgtttaag aaaccaattc agcaggtgcg
     3001 ctaatatcac agaattttac gagcatgcga tacttgcttt cattgataag ttgatagtct
     3061 cgttggtcat aactttcaac atacacgaca gccgtgttga tatatgtacg ttcaatacag
     3121 ccgattagtt catgagatac tccgatcggt ttgcatatat aagatcttcc tacttctgct
     3181 tttaccattt cattccctct tttctttatt tttattttgt tttttcttta ttttataata
     3241 acaaattacc ttgtatttga aaacgttttt atccattttt ttatttttta ttttgagtat
     3301 ctttctgctt tttagataac tattttttaa cacgatctta gacgttgtga caaaaacgtt
     3361 tgtttatatt cctatgttgt tcgtgcgttt ttttctttat attgctaagt tatttataaa
     3421 gaggtgagca tatgatttat attcaagcaa tagtatttgt aaccttgatg attgggctac
     3481 ttatcggatt aaaagaaaat agctaaacca tttaagaaaa aataaataag gaaaatgata
     3541 aatgcttttt aattgcatca aaaaacgtac gatgctaaac ttggtataag ttcgaaagtt
     3601 taaaggaaac tttcaagaac taaattacat tacaaaggag atgaatttta tgacgtttac
     3661 aaaaacaaat atgcaatacc gcgtagcttt tgatacaact tctaaccgtt ttatggcgat
     3721 tgatgcacaa aacgaaaata acgttgcgtt tggtattaca atcgaacaag cagtccgagc
     3781 attaaacgaa tctagataag gagtgaacca ttccaaatgg cgactgatga gatctcggaa
     3841 aatcaataaa agacgtttgt aacggtaatc cgcgacaaac gtctttttgt attcattaca
     3901 tatatttgga aattgtctct ctttatctct agacctaaac aaaaacacaa gttctaatat
     3961 caaccatctc gttttttatg acaattttgt tagctgtttc gatgataata ttccgttatt
     4021 atctgtataa tatttaatat aatcttaatt gtcaaattaa gttagacaga tcatcaatac
     4081 ttacgtaact caaaaatact tccaaaggtg ttcgataatc cagtgatttt ctaggaatat
     4141 tatttcgttt agatgcg
//
LOCUS       DACDIG010000133         3874 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.135, whole genome shotgun sequence.
ACCESSION   DACDIG010000133 DACDIG010000000
VERSION     DACDIG010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.135"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..328
                     /locus_tag="IV247_002792"
     CDS             <1..328
                     /locus_tag="IV247_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP8778791.1"
                     /translation="LRLPPRKERVYGQNFYADDLSMHLVFDMASQEDSKSGGARNILH
                     LVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDSNAEIIAKPSVAESKENKIV
                     FSIGGA"
     gene            331..>3874
                     /locus_tag="IV247_002793"
     CDS             331..>3874
                     /locus_tag="IV247_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778792.1"
                     /translation="MKFKKYLLQNLRIKRWVVRGLVIGGVLAVTLGAPFGMNDPTPDN
                     QDSKQETVINKNVAYAESKPAEQGMWTLYARILMGDKEVNDSAAKTGQMNLLGQGGIS
                     ADFPYSDISKQGNSIAGGNAGTQLASTLATYSYYGYIETVSGNAIASQASSILGSIGR
                     TIGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGEGGSDVSNPVSKALTSLL
                     NNLGLNNELFKAIASLSMLFFTAILGYQGMKLLSKDGVNSKGLSPVLEKWIVRIFVLF
                     IFLPVAGSLLGVYINGIQSAKKDGDIPTNVVSDYIINTRLWASTQNLAPSSDTGGDVP
                     DGKPTSEIKFVDTDYEPSKKSAMIRAINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NINTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGSAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTAHNFDATQPWGVANNATFSTQSVALLLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFIRIWEVVLTGSLASGAGVMAISLGVVTTSLIGLQLPIFLITFIRAFGDTAV
                     GFIPNTLVGDGVANIVRSAMILGVGYLLVFKKGEKVNNLINMLIGLPLEVAFGFDERA
                     KLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGNSSVKDMVAGGLGAVGAQKMAER
                     LNNMGDSNADAKSIADKVIPETPEGMSFNGGGVGSTINNTSFKDLVRQDQLRNNSQQY
                     IDHLGRMVDENGNFVDENGEILKDSKYGKDAKGNFIDEKGRLVDEDGNLIDTNGKPLE
                     DSEDGNIGMDSEGRLIDEKGRLVDEDGNLVDENGRRLLNGDFGHDSNGRLVDEKGRLV
                     DEDGNLVDEFGNKLGKHSDGYNVNGDLVDEDGNIIETRDALVADRNRELEQQRHTPIA
                     VAIPENEEDEITLDQTHQELRNEMSGEDVSDRVHRELGDGASGESQSRELNSNFDNSA
                     IRNDTVLKDRESLTKETDKEQSDVKKKFIPTPNVNQTDKYETNIGSGMSKDNQSIREP
                     ELTNKNNRTNNSKSTSDNSGKSNQSTKKTSTAKSAVEYLSKTGKNVYTSADKALAKAP
                     KGSLAGITYEAKNEAIKIGKGTAKVAKATATGASK"
BASE COUNT         1322 a          592 c          865 g         1095 t
ORIGIN      
        1 tttacgtctt ccacctagga aagaaagagt atatggtcaa aatttttatg cagacgatct
       61 ctccatgcac ttagtttttg acatggcttc acaagaggat tctaaatcag ggggtgcaag
      121 gaatatcttg catttagttg aatctgagtt tgaagaagcg attactgaag caataattac
      181 ttatccaaac gcaaaaatat tcgatgttaa gttgagtgaa gccggagcgt ttgaaggaaa
      241 aggtgattct aatgctgaaa ttattgcaaa gccttccgtt gctgaaagta aagaaaacaa
      301 gattgtattt tcgattggag gggcgtagct atgaaattca agaaatattt actacaaaac
      361 cttagaataa aacgatgggt cgtaagaggt ctagttattg gaggagtatt agcagttact
      421 ttaggcgctc cttttggaat gaatgatcca actcctgaca atcaagatag caaacaagaa
      481 acagttataa ataagaacgt tgcatatgcg gaaagtaaac cggcagaaca aggaatgtgg
      541 acactatatg ctcgtatcct aatgggagat aaagaagtta atgatagtgc cgctaaaact
      601 ggacaaatga atttgttagg acaaggtggt atcagtgccg atttccctta ttctgacatt
      661 tctaaacaag gtaattcgat tgcagggggt aatgcaggta ctcaactagc atctacttta
      721 gcaacttata gttactacgg atatattgaa acagtctcag gaaatgctat tgccagtcaa
      781 gcatcatcca ttctaggaag tattggtagg acgattggta gttttatcgg atcaattagt
      841 ttaatccttt ctaatattgc tgaatccata aatgaactcc ttggaaaatt tttcatgagt
      901 atgaatatat tttctcttat ggggtgggga gaaggtggat cggatgtatc taatccggta
      961 tctaaagcat taactagctt gcttaacaat ttgggattaa ataatgaatt atttaaagcg
     1021 atagcaagct tatctatgct ctttttcaca gcaatactag gttatcaagg aatgaaatta
     1081 ttgtcgaaag atggtgttaa ctcgaaggga ttatcaccag tacttgaaaa atggattgtt
     1141 cgtatttttg tattgtttat attcttacca gtggcaggat cgctattggg agtttatatc
     1201 aacggcatcc agtcagcaaa aaaagatgga gatataccaa caaatgttgt ttctgactac
     1261 atcattaaca caagactttg ggcttctact caaaatcttg ctccatcatc tgatactggt
     1321 ggtgatgtac ctgacggaaa gcctacatcc gaaatcaagt tcgttgatac agattatgaa
     1381 ccatctaaaa aatctgcaat gataagagcg atcaatacta gcagttatgc cagacaaaat
     1441 aatgatctag attctaattc agtcggtatg gctctattaa aagaatggtc atcgaatgat
     1501 actttcaata ttaatactta tgccggtgat gttaatgtcg ccgaaaatga tgcttatatg
     1561 ggaagagcgc ctgctgtttg gacaggctat aatttagggg atggaaaaga aggaagtgca
     1621 attaagaaac caaccaattt aaatggatat atttggtcag caaatcaaaa tgtgacagac
     1681 gacaatcgca agcctgatgg aaaaggaaca gcgcataact ttgatgctac tcaaccttgg
     1741 ggcgttgcta acaatgcaac ttttagcaca cagtcggtag ctttattgct ccaatcggca
     1801 cttgatacat cgggagctaa gttttatgcg tataatatcg ctccttcagg aattcaaggc
     1861 agtgctaaga atatgtcaac agtaaaaaca gaatggcgta cagcatcctt agttggtcat
     1921 ggagctatgg gtaagtttgg ctcgtggcta gcgatgaacg ctgaatcaat cgttttagca
     1981 gttctttact tagcatgtgc ttgggcgatg ttcaatgtga atatcgtgaa agcatttaaa
     2041 cgtgaattta ttcggatttg ggaagtagtt ctaacaggat cacttgcatc aggagcaggt
     2101 gtgatggcaa tttcattagg tgttgttaca acatctctga tcggactaca attacctatc
     2161 tttctgatta cttttattcg tgcatttggt gatactgctg tgggattcat tcctaataca
     2221 ttagttggcg atggcgtagc aaatattgtt cgatctgcta tgattttagg tgtaggatat
     2281 cttttagtct ttaaaaaagg cgaaaaagta aataatttga ttaatatgct tattggctta
     2341 ccacttgaag tagcgtttgg gtttgatgaa cgtgctaagt tattatttgg taatagaaat
     2401 gatgctgtgc gagttgtgga cggcgtttct aaagcaatca agaaaagcca aggagctaaa
     2461 aatccctatt atgatggtgg caacagctca gtaaaagata tggttgcagg aggtcttgga
     2521 gcagttggag cgcaaaaaat ggctgaacgt ttgaacaata tgggagatag caatgctgat
     2581 gctaagtcca ttgctgataa agtaattcct gaaacaccag aaggaatgtc attcaacggt
     2641 ggcggagttg gttccacaat caataatacc tctttcaaag atttagttcg tcaagatcaa
     2701 ctacgtaata actcccaaca atatattgat catttaggac gtatggttga tgaaaacgga
     2761 aactttgtcg atgaaaatgg tgaaatatta aaagatagta aatacggtaa agatgctaaa
     2821 ggcaacttta ttgacgaaaa agggcgtttg gtcgatgaag atggaaacct tatagatact
     2881 aatggtaaac cattggaaga tagtgaagat ggtaatattg gtatggatag cgaaggacgt
     2941 ttaattgatg aaaaaggtcg cttagtcgat gaagatggaa atcttgttga cgaaaatgga
     3001 cgcaggttgc taaatggtga ttttggtcat gatagtaacg gacgtttagt tgacgaaaaa
     3061 ggtcgtttag ttgatgaaga tggaaatctt gttgatgagt ttggtaataa acttggtaaa
     3121 cacagcgatg gatataatgt gaatggagat ttagtcgatg aggatgggaa tatcattgag
     3181 acgagagatg cgcttgtggc agatcgaaat agagaactcg aacaacaacg gcatacgcca
     3241 attgctgttg caattcctga gaatgaagaa gacgagatta ctttagatca aactcatcaa
     3301 gaactgagga atgaaatgag tggtgaggac gtgtcagaca gagtgcatcg ggaattagga
     3361 gatggagcat ctggtgaaag tcagtcaaga gagcttaaca gcaactttga taatagtgct
     3421 attcggaatg acacagtact taaagacaga gaatcgctaa caaaagaaac ggataaagaa
     3481 caatcagatg tgaagaaaaa gttcattcct actcctaatg ttaatcaaac agataaatat
     3541 gaaacaaata ttggatcagg aatgtcgaaa gacaatcaat caattagaga acctgaactg
     3601 acaaataaaa ataatagaac aaataattct aaatctactt cagataattc agggaaatct
     3661 aatcaatcaa ccaagaaaac atcaacagct aaatcagccg ttgaatatct atcaaaaact
     3721 ggtaaaaatg tttacacctc agcagataaa gctttagcaa aagctccaaa aggtagtcta
     3781 gcagggataa cctatgaagc taaaaacgaa gctattaaaa taggtaaagg aacggctaag
     3841 gttgccaagg ctactgctac aggagcatcc aaaa
//
LOCUS       DACDIG010000134         3829 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.136, whole genome shotgun sequence.
ACCESSION   DACDIG010000134 DACDIG010000000
VERSION     DACDIG010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.136"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(91..1002)
                     /locus_tag="IV247_002794"
     CDS             complement(91..1002)
                     /locus_tag="IV247_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8778793.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(1459..1887)
                     /locus_tag="IV247_002795"
     CDS             complement(1459..1887)
                     /locus_tag="IV247_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778794.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(1915..2553)
                     /locus_tag="IV247_002796"
     CDS             complement(1915..2553)
                     /locus_tag="IV247_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8778795.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(2557..3327)
                     /locus_tag="IV247_002797"
     CDS             complement(2557..3327)
                     /locus_tag="IV247_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAP8778796.1"
                     /translation="MIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQE
                     ISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLK
                     IKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFS
                     FFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIER
                     EPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
BASE COUNT         1159 a          721 c          567 g         1382 t
ORIGIN      
        1 aattaaacta ctgttgattt agttctaata aaaagattta gatgaacagt tttattgatt
       61 taaatagttc atatttttta tttataccgc ttatttacta cttgctgtac tagaatctga
      121 agtgaatgta ctcaaaacat tttcaaagtc tttgtctttg atttttacat tagcgtcttt
      181 caatacttcg ccaattactt tattctggaa ggtactgtca cttaattttg tgtctgttgc
      241 aatttctttc aattcttttt tgtatttatc catgtcgttg cctttgtttt ggtttttcac
      301 cattttaacc acataatatt ccgtagtata agttgaagca ttggtagaag tgatcacatc
      361 agaaacttga ccatctttca acttaaaggc tgcttctttg acttctgccg gaaccgttgt
      421 agtagtggaa tcaaatttca cttttccgcc atcttctttt gtcgtagtat cagttgactt
      481 gtctttggct aatttagaga aatcgtctcc cttatcagca gctttcttca catcttttgc
      541 atcatcttca cttgctactt ggataatttg agcttccact tctggatgga aagattccca
      601 agccgttttt aaatcttcat cggtcaattt gatatgtttc tttagacctt cttgaaaggc
      661 tagactttgt ttgatactgt ctttgaatgt ttgttctgtg taacctgcag atttcaattg
      721 tgaatcaaat gaatcaccca attgtttctt tgtttgatca tattgagaat cgatttcttt
      781 ggttgatacg tcatctccat atttatcttc aaatacttga taaacgatca actgttgcac
      841 agcggaatct gctccttgca tttttttcaa gctatccatt acttcttgtt cgctgattgt
      901 accgccttta taactaacgg ctgttttttc accggcattc gtacagcctg acaataagcc
      961 agtgcctagt acgatggcaa gtgctaaaaa tctttttttc atttatcaat tctcctactt
     1021 ttcgtttttt gcgaattgct aaaaacaaag gtgttcaaga tgaaaatcat caagaacacc
     1081 tttactaatc tgctcgatac gtcctgaata gatataaggc tacacaatcc agaaagttcg
     1141 ctcagcagaa taaatgtcaa gtagcacatc acaccttaat atactacttc ttttgttacc
     1201 gtttttcaag tctatcttgc gttccgtatt taacgtaact aattcttaga acaaatgtta
     1261 tcgattttgt ttttcttctt ccgctgctgg gatattcaaa tcagacactt ttatgttcga
     1321 ttttccagac atttttttgt tgtttttatt atgatatatt tttatacaac taaaaatgaa
     1381 tatattaaaa ctaccaaaaa taacttttaa cgctgtttca ttatatgttt tttctatttt
     1441 atataaactt ttggatactt aataccaacc ggttggattg taccaacatg agaatgtgtc
     1501 agttctattt cctgtcgcac ttgcataggc ccatactcct tttgttgcat ttgctgatcg
     1561 aatagagttt ccaactttcg cttttgcacc gtgacgatta tagacacgac tatagtggtt
     1621 agaatgacct gtttttctcg tacaagttgt atcactataa taaacccatc ttccagagct
     1681 atgattaatt gctgttctag aaatttcccg gtctactggt aaatttgact taataatatt
     1741 acctttagca tcaaaatgaa tctctcctgg ctttaaagat ttgtaataga tttcaccgtg
     1801 aactgaattt gcttccactt cagatgacat agcgtttcct ccaacaaaac ctatccctag
     1861 tgcacaaata ataactgctt ttttcataaa acgacatcct ttcctttttg atttttaaag
     1921 catgatcttc ttgtttccta ttttggtcag atatggatca tgcgtgacta cgataactaa
     1981 cgtaccttgg gaagcttgtt gcgataagat ttcaactact ttatccttat ttcttttatc
     2041 caatgaccca gtcggttcat cggctaaaat gattttgcta ttcttcaata agactctcgc
     2101 acaggcgatt ctttgcttct caccaccaga taatgtgtag ataggcgtac ttagcttctt
     2161 gtttatattt acttttttca atgcctcata ctttgctgtt cgcttatctg aaacatttaa
     2221 attcgaaaac ttcaatccaa tatctaaatt aaaatcgatt gtttcatatt caatcaaccc
     2281 aaaatcttga aaaagaaaag atatggtatt tctttttatc aagcgcttct ctctcccagt
     2341 taattgtttc gtctcttgat cgaacaaata gaattgtcct gaactgaact gatcattcaa
     2401 tcctaatata ttcaataacg tacttttacc tgctccactt tctccaacaa cagtcacaag
     2461 atccccagca tccgcagtga aacgatccaa atgaagaatc gttttgtttc gaatgatttt
     2521 tttcgcacct tttatcacta tcaacggctc caattgttaa cctccattca tcagcaataa
     2581 ctgacttttc ttttctgtag acaaaataaa aacaactgaa agaataaaat caatgaagct
     2641 aaagaaaaaa atattaaaaa gatatttagg ctctctttct ataagaaaaa ggaagattac
     2701 acttgtcgtt ccccatttga tgaggttcat catgaagaaa tctttatacg tttcaaagag
     2761 cgaataacca cttgaacgaa agatagcgat tttccgacaa ttgtagacaa aatatatgca
     2821 taacatgaca tagctcaaca agaagaaaga aaagaaagtg agtaaaaata tctgtatcaa
     2881 gtaatgaaat gatctgctta acactttgac agctatctct tcagtctgag tatacggaaa
     2941 tgataactgc aagttatcac gtaactgact ttcttcaatc agctcaaata acctttcttt
     3001 cgacaaagag cctatcttga tctttaacgg tggatagatt tctccgctga gaatatttct
     3061 ttccaaatac atactgtttt tcaatgtcag tatttcaaca accggataat gttcgatcca
     3121 ggggatagaa ggaataaatg tataaatggg ttgattgttt tttataaata gaaatttcgg
     3181 ttctttttca ctgatttctt gataatattc tgttatttgc gccaggtcat tttttaaatc
     3241 tgtatgtcta tatcgttcag ggatcaatat aacagggtgc gtttcttttt cggttatttg
     3301 gatcaacctt ccagagacat caatcaaagg aaatttctta agataattca gatttatttt
     3361 tatccctctg ccaaactgtt cattttcttc attcaagtaa tctgatacat tgactaatat
     3421 acttccgttt ttattcaaag tttcatataa ttcctgatct gctgctgatg caaaacgatt
     3481 ggaataaata aaatccacgt ggtttttccc gatggttact ggataaaata catgatagtc
     3541 ctcaccagcg atttcttttg gtggttcatg ggaaacagct aactgtgaag aagaaaaaac
     3601 gatagttgct aattcgacat tggcaacggt caaaagaaga gaaaacaaca atccaaatgt
     3661 aaaaagaatg tattggatat agttgaacgc ttctcttaac gaagcgattt tttcaactaa
     3721 taaaagagaa atcaaagaga taccatatat tccgctgaac aagaaaacgt acatctggaa
     3781 caaataaaga aattgctcct tataatacat aaaaataaaa ccaccaatg
//
LOCUS       DACDIG010000135         3750 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.137, whole genome shotgun sequence.
ACCESSION   DACDIG010000135 DACDIG010000000
VERSION     DACDIG010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.137"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            120..1142
                     /locus_tag="IV247_002798"
     CDS             120..1142
                     /locus_tag="IV247_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8778797.1"
                     /translation="MFKDKILLITGGTGSFGNAVMKRFLDSDIKEIRIFSRDEKKQDD
                     MRKKYNNDKLKFYIGDVRDINSVKNAMYGVDYVFHAAALKQVPSCEFFPMEAVKTNIM
                     GTDNVVTAAIEMGIQKVICLSTDKAAYPINAMGISKAMMEKVFVAKARTVTDERTMIC
                     GTRYGNVMASRGSVIPLFIDQIKNGKPLTVTDPGMTRFLMSLEEAVELVVFAFQNAKA
                     GDIMVQKSPASTVGDLAQAIKELFHADNEIHIIGTRHGEKRYETLLTKEEYLVAQDMG
                     DFYRVPADTRDLNYDKYFVEGSKELTQEGEYNSDNTQRLSVEQIKERLLELDYVRKEL
                     KEWQNQ"
     gene            1139..2248
                     /locus_tag="IV247_002799"
     CDS             1139..2248
                     /locus_tag="IV247_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CapF"
                     /protein_id="HAP8778798.1"
                     /translation="MNILVTGSNGFIGKNLVAELKNEGFTDILSFDRDTPVHLLDEYC
                     EKADFVFHLAGVNRPKDDAEFMTGNFGFTSELLEKLKEHGNTCPVMISSSIQAELNNS
                     YGKSKKAGEDLLKKYQKETGAPVYIYRFSNLYGKWSQPNYNTVVATFCYKIARDEEVT
                     VNDPSAIIELCYIDDVVKELLHCLNGQPYTENGYCAVRETDEVTVGELKELITSFKNS
                     RTNLSVPNLARRVEKNLYSTYLSFLPEDEFSYFLKMNVDNRGSFTEFLRTKDRGQVSV
                     NISKPGITKGNHWHHTKNEKFLVVSGEGVIRFRKVDSNEIIEYFVSGEKLEVVDIPVG
                     YTHNIENLGSTDMVTVMWVNEPFDPEHPDTYYVEV"
     gene            2252..3376
                     /gene="wecB"
                     /locus_tag="IV247_002800"
     CDS             2252..3376
                     /gene="wecB"
                     /locus_tag="IV247_002800"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HAP8778799.1"
                     /translation="MKKLKVMTVVGTRPEIIRLSAVINRLDRSEAIDHILVHTGQNYD
                     YELNEVFFEDFKLKKPDYFLNAATGTAIETVGNILIKIDPILEEVKPDAFLVLGDTNS
                     CLCAIAAKRRHIPIFHMEAGNRCFDQRVPEETNRKIVDHTADINLTYSDIAREYLLRE
                     GLPADRVIKTGSPMFEVLNSRKDDIQHSDVLNRLGLKEGQYFVVSAHREENISSETNF
                     LHLVDSLNTIAETYQLPIIISTHPRTMKMIEAKEIKFHELIQTMKPMGFNDYNKLQIN
                     AKAVLSDSGTISEESSILNFKALNIRQAHERPEAMEEASVMMVGLEKERIMQGLEVLE
                     TQQKDTLRHVADYSMPNVSEKVLRIILSYTDYVNRVVWGR"
     gene            3373..>3750
                     /locus_tag="IV247_002801"
     CDS             3373..>3750
                     /locus_tag="IV247_002801"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAP8778800.1"
                     /translation="MRIVVNDIAASEGGAMSVLRDLHSDIISSGDENEWIFLLGEKFI
                     EESDNIKVLTFPKIKTSWIKRLDFDFISGKKIINKLQPDIYLSLQNTATIGVKAPQYV
                     FLHQPLPYQKEKNFSFIKKKEFKM"
BASE COUNT         1375 a          581 c          744 g         1050 t
ORIGIN      
        1 aaaaaataag aaacgaatta ggtaaaaatg cttttattta tttgaaaaat aattttgatg
       61 taaaaagaag ctattctata attatgaatc attttgaatt ataaaaagga gcaatttaaa
      121 tgttcaaaga taaaatttta ttaatcacag gcggcacggg ttcttttggg aacgcagtga
      181 tgaaacgctt tttagattca gatatcaaag aaatacgtat cttttctcgt gacgagaaaa
      241 aacaagacga tatgcggaaa aaatacaata atgacaaatt gaagttttac attggagatg
      301 ttcgggacat caacagtgtc aaaaatgcga tgtacggcgt tgattatgtc ttccatgcag
      361 cagcacttaa acaagtacct tcttgtgagt ttttccctat ggaagctgtg aaaacaaata
      421 tcatgggaac agataatgtc gtaactgcag caatcgaaat ggggattcaa aaagttattt
      481 gcttgtccac tgataaagct gcttatccaa tcaatgctat ggggatttct aaggctatga
      541 tggaaaaagt atttgttgct aaagcaagaa ccgttactga cgaacgcacg atgatttgtg
      601 gcacacgtta tggaaatgta atggcttcta gaggatcagt tataccatta ttcattgacc
      661 aaataaaaaa tggtaaacca ttaacagtga cagatcctgg catgacaaga tttttgatga
      721 gtttggaaga agctgtagag ttagttgttt ttgccttcca aaacgcaaaa gcaggggata
      781 tcatggttca aaaatcacca gcttctactg ttggcgacct agcgcaagcc atcaaagaat
      841 tattccatgc agacaatgag attcatatca tcggaacgcg acatggcgaa aagcgttatg
      901 aaacactact gacaaaagaa gaatacttag ttgctcaaga tatgggtgat ttctatcgtg
      961 ttcctgcaga tacacgtgat ttgaattatg acaagtattt cgttgaagga agcaaagagt
     1021 tgacacaaga aggagaatac aattcagata atacacaacg tttatctgta gaacaaatca
     1081 aagaaagact tcttgaactg gactatgtaa gaaaagaatt gaaagagtgg caaaatcaat
     1141 gaatatttta gtaactgggt caaatggttt tattggaaaa aatttagttg ctgaattaaa
     1201 aaatgaagga ttcacagaca ttcttagttt tgatagagac acgcctgtac atttattgga
     1261 tgaatattgc gaaaaagcag actttgtttt tcatttagct ggagtgaaca gaccaaaaga
     1321 cgatgctgaa ttcatgacag gaaattttgg ttttacttca gaattgctag aaaaactaaa
     1381 agagcatggc aatacatgtc ctgttatgat ttcctcttct atccaagcag aacttaataa
     1441 ttcttatggc aagagcaaaa aagccggaga agatctatta aagaaatacc aaaaagaaac
     1501 aggagcacct gtatatattt atcgcttctc aaacttatac ggtaagtggt cgcaaccaaa
     1561 ttataataca gttgtagcca cattttgtta caaaatcgca agggacgaag aagttacggt
     1621 aaatgatccg tctgctatca tagagctttg ttatatcgat gatgtcgtga aggaactact
     1681 ccattgtctg aacggacaac cttatacaga aaatgggtat tgtgctgttc gtgaaacgga
     1741 tgaagtaacg gtcggcgaat taaaagaact aattacatct ttcaaaaata gtcgaacgaa
     1801 tttgagtgtt cctaatcttg cgagaagagt agaaaaaaat ctgtacagta cgtacttaag
     1861 cttcttacca gaagacgaat tttcttattt tctaaaaatg aacgtagaca atcgaggatc
     1921 gtttactgaa tttttaagaa caaaagaccg cggtcaagta tctgtcaata tttccaaacc
     1981 gggaatcact aaaggaaacc attggcatca tacaaaaaat gaaaaattct tagtagtaag
     2041 cggagaaggt gtaatccgtt ttagaaaagt agactcaaat gaaataatcg agtattttgt
     2101 ttcaggagaa aaattagaag tagttgatat tccagtaggt tatacgcaca atatcgagaa
     2161 tctgggatca acggatatgg tgacagtgat gtgggtaaat gagccatttg atccagaaca
     2221 tcctgatact tattatgtgg aggtctaatc aatgaaaaaa ttaaaagtga tgacagtagt
     2281 aggaacccga ccagaaatca ttcgtttgtc tgctgttatc aaccgtttgg atcgatcaga
     2341 agcaatcgat catattttag tacacactgg ccaaaactat gactatgagc taaatgaagt
     2401 attcttcgaa gacttcaagc taaagaaacc cgattatttt ctaaatgcag caactggtac
     2461 agcgattgaa acagttggaa atattttgat caagatcgat ccaattttgg aagaagtaaa
     2521 accagatgca ttcttagtat taggggatac aaatagctgt ttatgtgcaa ttgcagccaa
     2581 acgtcgccat attcctattt ttcatatgga ggcaggaaat cgctgtttcg accaacgagt
     2641 accggaagaa acaaatcgta aaattgttga tcatacagct gatattaatc taacctatag
     2701 cgatatcgcg agagaatatc tcttacgtga aggattacca gctgatcgtg taatcaaaac
     2761 aggaagtccg atgtttgaag ttctcaattc aagaaaagat gacatccaac attccgatgt
     2821 ccttaatcgc ttagggttga aagaaggaca atattttgtt gtttctgctc accgcgaaga
     2881 aaatatcagc tcagaaacaa atttcttaca cttggtagat agcttgaata caatcgctga
     2941 aacttaccaa ttgccaatca tcatcagtac gcatccgcga acaatgaaga tgatcgaagc
     3001 aaaagaaatc aaattccatg agctgatcca aacaatgaaa ccaatggggt tcaatgatta
     3061 caataaattg caaatcaatg caaaagctgt actaagcgac agcggaacga ttagcgaaga
     3121 atcttccatt ttgaatttca aagcattaaa catccgtcaa gcacatgaac gaccagaagc
     3181 aatggaagaa gcaagtgtca tgatggtagg attagaaaaa gaaagaatca tgcaaggact
     3241 ggaagtattg gaaacgcagc aaaaagatac tttgcgacac gtcgcagatt attcaatgcc
     3301 aaatgtttcg gaaaaagtat tgcgtatcat cctttcgtat acagactatg tgaatagagt
     3361 ggtgtgggga agatgagaat tgttgtaaat gatatcgctg cttcagaagg tggggcaatg
     3421 tcagtattga gagacttaca ttctgatata ataagtagtg gtgatgaaaa tgaatggatt
     3481 tttttactag gtgaaaaatt catagaagaa agtgacaata taaaggtact aacttttcct
     3541 aaaataaaaa ctagctggat taaaagattg gattttgatt ttattagtgg aaaaaaaatt
     3601 atcaataaat tacagcctga tatctattta tcattacaaa atacagctac tattggtgta
     3661 aaagctcctc aatatgtttt tttacatcaa ccactaccat atcaaaagga aaaaaatttt
     3721 tcgtttataa aaaaaaaaga gtttaaaatg
//
LOCUS       DACDIG010000136         3735 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.138, whole genome shotgun sequence.
ACCESSION   DACDIG010000136 DACDIG010000000
VERSION     DACDIG010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.138"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            295..1542
                     /locus_tag="IV247_002802"
     CDS             295..1542
                     /locus_tag="IV247_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778801.1"
                     /translation="MNMKRNSLGMVLLASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNISK
                     DTLTVKEADGEVVSQTVQKDKPDPIENTEQENKKSETTKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTEADTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSKPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKPMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            1810..>3735
                     /locus_tag="IV247_002803"
     CDS             1810..>3735
                     /locus_tag="IV247_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HAP8778802.1"
                     /translation="MKHEKTIILKSKIIYCFATLVMMLSTLIMPFSAIRAEADVTKPT
                     SAKFIETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     EFSYTATNTIKKGKVKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGVFKQEGITVKDDGQTFEYTATNTLKKGLVKVHKVDQDGKPL
                     ANAEFTLTDEKGATQVEKTDENGLASFNIVANKTYSLEETGVPVGYTGAFKQEGITVK
                     DDGQTFEYTATNNLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATGLATF
                     NIIANKTYNLEETKVPTG"
BASE COUNT         1386 a          603 c          772 g          974 t
ORIGIN      
        1 gcaattatga cagtgcaatg gtactattat tatacaataa attttgcgaa aggggtaggt
       61 ctaaatcgca aacaaaaaga aattgaacct agacaaatac aacttgtata ccgcaaggga
      121 agcaagtttg attttgggta aaggtagaaa ttatgttaca aacataatca actattatcc
      181 aaatcgtttg cctaaaggtt cgttcaagaa agtgggtgac aaaacctata ttataacgga
      241 agaaggaata gaagcattgc gatcacaaac agaaaataaa aacaaaaaga aggaatgaat
      301 atgaagagaa attcgcttgg tatggtttta ttggcatcag ctgtacttgc ggggttatca
      361 gtaccagtaa cctcacatgc tgatgaatgg actgcaaaca caccggatca aatccaaatt
      421 acaacaggac aatcaagtta tgcgttcaaa aaaggagata ctatttggtc aactgtagag
      481 aagcttaaaa gcttaggtta caagactact gttgctgagt ttgccgaatt gaacaatgtt
      541 gatttggcaa aaggagaaca acggactatc ccgattggac gaactttcaa catcagtaaa
      601 gatacattaa cagttaagga agcagatggc gaagtagtta gtcaaactgt tcaaaaagat
      661 aaacctgatc ctattgaaaa tacggagcaa gagaacaaaa aatccgagac tacaaaagaa
      721 tcggaaccaa cagcaaacac ttctgataag acagaaacaa gtgcttcaag tgttgatgga
      781 gcaagttcat ctaatacaaa taattctaca agtgagaaag aagcatcaca gggaacaagt
      841 gaaagtagca ctacggaagc tgatactgga acatcaacaa ataactcaca atcaacatca
      901 aatgatgcaa atgaatcaac cagtacatct tctagtgatc aatcacaaac aggttcgagt
      961 gatgaaactg acagttctac ttcaccaagt aaacccgaac aaccaactaa tgttgtgcta
     1021 ggcgttccat ttattagtca aggtaacaca atgctttgcg aaggaacatc tttgctagaa
     1081 gcgttacatt ataaaggtgt tgcaacaaat caagatttaa tgacttttgt taaatcaatg
     1141 ccactatctc ctgacaataa tccaaataat ggtttttctg gtgaatggag acacaatgtg
     1201 gatggaactt atcaaggtat gaatccggct cctgtagttt catggggtgt tgctaatggc
     1261 ggaaatgttg tagatatttc tggttcatcg gtacaaggat taaaagatga gattgtaaat
     1321 ggaaatcctg tagtagcgtg ggtaacttat gcctttgaac ctgctcaaca taaaccaatg
     1381 ttttggggcg atgctatttg gaatcgacat gtagtagccg ttgatggatt taaagacggt
     1441 ttttaccatg ttgtcgatcc tgtgtttggg gcaagttgga ttaatgcagg atcatttgaa
     1501 acagcatata acgtatctca attagcggtt tcggtacgtt aataaaagat atcgtttata
     1561 tactaagtag ttatatagtc tttatttgtc cttgcttaac tacttagaaa acttgttgct
     1621 catggtgggt agaactaaaa ggggtgatgc tatgaatact agagcgttga aacagcttgc
     1681 tttcaacgaa aaaaatgatt aataggtgtg gtgattttaa cttaggaatg ggcgcgaagg
     1741 tcattaccac accgacatat cgtggctcat tttcttcgcc taactggtat aaagaaggag
     1801 taacgagaga tgaagcatga aaaaacgata attttaaaat caaaaataat ttattgtttt
     1861 gcgactttag taatgatgtt atctacgctt ataatgcctt tttcagcaat aagagctgaa
     1921 gcagatgtca caaaaccgac aagtgcaaaa tttatagaaa ctggcgtaaa aacagacggt
     1981 tatattaggg ttaatatgcc gaatcatcca aatgaatgga tgatttcaag tcaatttaaa
     2041 gattctcatg gaaatattgg atattgtatg gattcagaat tgccatcccc aacaggaagt
     2101 ggagccggaa gcttgaagta caagggagct ggcagtgatg agttctatcg tatgtttaaa
     2161 ggtggttttc catcaaaaac agcaaaggaa ctgggtgcag gaaatgatac agaagcatgg
     2221 tatgcaacac aattagtttc atgggtttta gccggaaact ttaaagtttc acagattgta
     2281 tggtcacatc caaaccatac cgccgcagaa acagctcgtg taaaaaaagc atttgaaaaa
     2341 atttatgact atgctaagaa tggaaaagat acgcccaaca ctgagttttc tatcacagct
     2401 tctaagacag ctgacgaagg aaaatatcac actttcacat ataaaacagc atcaaataag
     2461 actggtaatg cgaagctcac ttttacatca gcaaaaccag ctggtatgaa gatttatgat
     2521 gcagatggta aagaaattac taacaacact gtgaaattaa attcaagttt tactattaaa
     2581 gtacctgtga ctacaccaag tggtactttg tcatttaaag gtactgctaa tgttagcaca
     2641 accaatcctt ttacttttga tggacgaggt gtgtatcaag atgccgtagt tatgatcact
     2701 acttcagaaa caaaggactc taaatctttg agtgctaaat ggacaagagc tgttggtcag
     2761 gtaacagtta agaaagttga tcaagatgga aatgctttaa aaggagcgga gtttactcta
     2821 actgacaata acaatgctaa atctgttaag acaaccggag atgatgggtt agctacgttt
     2881 gatttgcaac aaggcttaac atacagctta gaagaaacaa aagttcctga aggttatcaa
     2941 ggttcattta aaaaggatgg tattactgtt aataccgatg gacaagaatt tagttacact
     3001 gctacgaaca caattaaaaa aggtaaagta aaagttaaaa aggttgacca agatggaaaa
     3061 ccattagcta atgctgaatt taccttgacg gatgattcat cccaaacaca agcagaaaaa
     3121 acagatgaaa acggacttgc aagcttcgat attgtagcaa acaagaccta cagccttgaa
     3181 gagaccggag taccaactgg ttatacaggc gtattcaagc aagaaggaat taccgttaaa
     3241 gatgatggac aaacttttga atatacagct acaaacactt tgaaaaaagg tttagtaaaa
     3301 gttcataaag tcgatcaaga tggaaaacct ttggcaaatg cagagttcac tttaaccgat
     3361 gaaaaaggtg caacacaagt agaaaaaaca gatgaaaatg gacttgcaag cttcaatatt
     3421 gtagcaaaca agacgtatag cctagaagaa actggagtac cagtaggtta cacaggtgca
     3481 tttaagcaag agggtatcac cgttaaagac gatgggcaaa cttttgaata tacagctaca
     3541 aataatttga aaaaaggtgt agtaaaagtt cataaagtcg atcaagatgg aaaaccttta
     3601 gcagatgcag aattcacctt aacggatgaa aaaggtgtaa aagaagttaa aaagacggat
     3661 gcaactggtc tggctacgtt caacattatt gcgaacaaaa cttacaactt agaagaaaca
     3721 aaagtaccaa caggt
//
LOCUS       DACDIG010000137         3553 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.139, whole genome shotgun sequence.
ACCESSION   DACDIG010000137 DACDIG010000000
VERSION     DACDIG010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3553
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.139"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            304..1344
                     /locus_tag="IV247_002804"
     CDS             304..1344
                     /locus_tag="IV247_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAP8778803.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
     gene            2168..2500
                     /locus_tag="IV247_002805"
     CDS             2168..2500
                     /locus_tag="IV247_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP8778804.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            3119..3460
                     /locus_tag="IV247_002806"
     CDS             3119..3460
                     /locus_tag="IV247_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP8778805.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1313 a          472 c          662 g         1106 t
ORIGIN      
        1 atgcataagg tagttcttaa tttatttttt gttttgacac tggtagtact taattatctt
       61 taattttaaa actaaatttt attcatacgt ttttctggta gtatttaatt ataaataata
      121 tttatttaaa gtagttctta atgactttaa aggtagtaca taattcattt tatttctgat
      181 tttaggtagt atttaattaa aaaacagttt taatgcgttt gcatttttta tttatctttg
      241 ctattttcga aaagagtagg taattttaca taaataaaac aacgtgaaag tagaggatct
      301 acaatgactg attttaaatt ttttaaggcg gatagagttt acaacgaatt attttatcaa
      361 tttccaaaag tctttattgt ttctgacgaa tacaaaaaaa tgaaagattc aactaagatt
      421 gcctatatgc ttttaaaagc aagattagag atcgcaatca gcaaacggca aatcgatgaa
      481 gaaggtaatg tttattttac ttatacgaca aatgaactat gtagagtatt aaactgccaa
      541 aaacaaaaag cgatagcaat caaaaaagag ttggaatcct ttggtttatt attacaaaag
      601 cagatgggat ttaacaaaca gttagggaaa aataatccta atagactata tctagcagaa
      661 ttaaaagtct cagaaaatga tatctactta ctcgaaaaat ttgatagaga gaatagggaa
      721 aacgttgata aatcagaggg tatgaaaatc atacccaccc tcgacgaaaa atcagacgct
      781 gaatcccttg gggctcaaga gggtatgaaa atcataccgt gccaaaacgt tgataaatca
      841 gagggtatga aaatcatacc agaacttaat aataatatat tagacactaa tagacacaat
      901 atagacactg aaaaagaccg cctacaagat caattgttgt tagacaattt tgagacaatt
      961 atgacaaacg acagcattgc tacgtttgtc cctgaacgag tattaaattt gataaaaaca
     1021 ttttcttcaa gttacagtga agctcaaaaa accgtccaga ctattcataa tgcaaagaaa
     1081 aaagctgaaa tagaaagtga tatttcgata gtttttgaag aactcgatag ttattatgtc
     1141 aatgcagaac aagaattata cacgacactg ttaaaagcct atcaaaaatt aaaaaccgaa
     1201 aaagtcgaaa atatccagaa cctgattttt gtctatgtaa aaaattggtt tatcgaaaaa
     1261 ccaatagctg ctaaagtatc aagtgaaaaa cgtttgaatt atgcaagctc cccaagcact
     1321 attacgaaag actggttaga gtgaaacttt actgacaaag tgattgttgc ttcatggagt
     1381 aacattaaaa ttaaaaattt tttattattc taattatatt acgaatatta caaaataata
     1441 gctattttaa tagaatttat aagttaaaat attatgataa aaatcacaaa cgtagccttt
     1501 tacgtcataa gtgttaaaag aaataattaa tttcaaatat agaaaaggga acgtatgttt
     1561 gcatactcct tttttttgag ttataataat acgcgaagag agataatttc attatttaaa
     1621 caagctaaaa atcttgatat tacttctttt caggcaatac gaatattatt cgcttacatt
     1681 ttttgtataa atttaattgt ctgctatgct tgtgatgaat tgattttcgt tttgcacttt
     1741 tggatgataa atttggacaa ttgaaaatac aaaaaaaaga aaggcggtat gaactcggaa
     1801 ggctggtaac cttcgtcagc gttcatacac ggcattaaga tttggccctt aatgcgttaa
     1861 cctttcagtg ttacggtatt acccgcaatg gatttagtat aacagatatt caaaagtgat
     1921 accacttttt ttggctgtgt taaggttaaa aatacgtgat attacgatga atctatgcct
     1981 aagcataatg ttataaaaat gtccgtgtgg ctctttgccg tatgacgaac gctgaaatta
     2041 gagggaacgc atgtttggat gcgttccctc ttttttgttt gtcatgaacg aaaatcaatt
     2101 ctctattttt gaaaaataac aagaaaaact tgttaaaaat atttaaagaa attgggtggt
     2161 ttattttatg tcatatacag gaattttgag tctcaaagat atttgtcact acggtaaacg
     2221 gtgtaccgct acagagaaaa tcacgaagaa actatcaact ggccaaagta aaaccgttgt
     2281 gcagtgtaag aaatatataa tccagaaaga caaagtctca gaggaaatga tttattacat
     2341 tgggaaacaa aagcaaataa ttttaaaaga tcctatcccc ttaaaagagc tatatccaac
     2401 aatcaaacat gtttatgacc aaaacggtgt actgattggt cgaagaaaaa atggagtctt
     2461 gcgttgtacc gcaaaaggga tggggcgttt gatcggctga attgatcatt ctcataactt
     2521 gtattttatc gtttttcgct tgcttgaaaa tattgtcgag acttctaaag gaacttagag
     2581 tcactagaaa aataaggagt agacttgatc tatcaacaat tcccttcggg gagtgacggg
     2641 gtaggtttta acgattggta acgtaaaaaa ttggtcaatc gagtaaatgc gcttgtttgg
     2701 atagtggtga aatgaatttg tgtaaataat aagcgcagag taaacaagag ggaatttaga
     2761 agttagacaa gttagaatag cgtctagatt aatcatgagc agctactaag ggatctagac
     2821 tttgacgcaa actcatggaa taaggagtag gtcttctcgt aatggggagg acgcacaaga
     2881 aattgtgtcg tgtgctgcag tacatgtaga tacttgcgag gacgttgatt attggatcaa
     2941 tcgagcattt accgctgtct actagggttt ctcgtttcgg acgccataaa cgagtcgaaa
     3001 ttttttatgt atttttaagt aaacgaaaaa cgataaaata tgaacaagtc ctgaataaag
     3061 tgaaaaaatt aatataaagc atagagaaaa tgaaatttac gtaatggagg aatgacaaat
     3121 gaaaaattat gatccgaata tacgattggg cacacatact attaaggttt ctttccaacg
     3181 ttgggattac aaaggatttg tgacgtttag aaggggcgga aattgtaaag gattagatgt
     3241 tttagcatta gacgaggatg atttatacga tcaaacattg actgataatc caattggatt
     3301 cggcttgcta cctgaagatg atgaaggtaa tgaatggttt aaaatgacgc ttatgaatga
     3361 taatggagac gaattatctg ttgaagatac ttggtcatat ttgagtgatt atattgtcag
     3421 ctttgaaatc attgaatttg tagcggataa agaagaataa aaactggagg aataaatttt
     3481 acagataaag gcttagaagg agtcacgacg attaataaaa aggtagtatt accttaggca
     3541 ttgagtaagg aat
//
LOCUS       DACDIG010000138         3472 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.140, whole genome shotgun sequence.
ACCESSION   DACDIG010000138 DACDIG010000000
VERSION     DACDIG010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.140"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            139..372
                     /locus_tag="IV247_002807"
     CDS             139..372
                     /locus_tag="IV247_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HAP8778806.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            369..776
                     /locus_tag="IV247_002808"
     CDS             369..776
                     /locus_tag="IV247_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP8778807.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(1913..2431)
                     /locus_tag="IV247_002809"
     CDS             complement(1913..2431)
                     /locus_tag="IV247_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAP8778808.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         1178 a          588 c          523 g         1183 t
ORIGIN      
        1 taaatttttt gaattttctt aattaattat atagaaagaa caatctttct atagagttag
       61 acatgagagg gactatgctt gatttaaaat caacaagcgt atatactgta tatacaaaga
      121 aaaaggaggg agaagactat ggcaattaaa gaaagaaaat ttagggaagt ggggaactca
      181 gtagtattaa ctatttcaaa ggagtttctt aaagaaaatg ggttaaatcc cggagatacg
      241 gtttttttag atgaagaaaa acttcgagcg gcaattacaa aaaaacaagc cttatctgat
      301 gaaattgatt tagcaatatc tcaagcatta actgaatatg atactacttt tagagagctt
      361 gtcgataaat gagtattcat tacctagaag ccaaagatat tataaaaatg aatgtaatcc
      421 aaataaaaaa atactctcct aatgaacccc ttggtgtaaa ggatgcgaat gctttagaaa
      481 tgtgtgtagg gcaacttcga gcctcagctt ttggtgaaga ggtttatcct tcgatttatg
      541 aaaaagcagc tatcctactt atacaattga taaagaaaca tccttttcac aatgcaaata
      601 aacgtactgc tttcttagct acttttgtct ttttgaaaat caatggacag cttttagcta
      661 ttgatcaaaa agatgctgta aatttagcgg tatatattgc tacgtatgat aaggatttcg
      721 atcaactaaa atatgaagtg atcaacacta taaagacaca tacgcagccc atttaaggag
      781 ttactgtatg tgtctttata gggttattag ctgctatagc gaaataattg aagtaaatta
      841 caaatagcta taattcaaat ccaactatat agaaaacgta aattttctat atagttggat
      901 taagataacg actatttatt ttatcatatt tttactatac cctatacttt tacttggtat
      961 atttatgaat aatcgcaaat ttacccctat actactagtt tgtaagttag attaataaaa
     1021 atactaaagt tatttgttct tttattaaat acacctacaa cttataaatt gatggtattt
     1081 agataacacg aaagaataaa aataacagct gatagtaaag tttgttgacg attgctactc
     1141 aatcgaaagt gagttagtgc gtatgaactt gcttctgaaa acttatgaaa tgaaagacct
     1201 tttacatctt gcagaaatcg gtactgggat taaactaagc aataaaaact aatgttcccc
     1261 tcattctttg aacttctact atttgattta tttattctga taaatgacga tttcaccttc
     1321 aggatcttca aatcccgtat agaatttatt taagatctct tcagtatcag taataggata
     1381 ggcgataggc cacataccct ttgatggtta gaataaactg ctcaaaatct gtatcgtatg
     1441 ttggctttag catattaaca aaggctatct ccaaattata aaccataagc gttgaatcgt
     1501 tcttaacaat ttcaattctt cctaacccaa agtcagaaga tataattacc ggacttgcct
     1561 tacccttcat gtgggttgtt gagattccat atttaaaggg aattgtcggt actatcggcg
     1621 gtctgaaaaa catcccaaaa ataaaaaaat ctttcccctg ctagtctcct aagtcccttt
     1681 gtagcggata tggcaccata atctcatttt atcgaagtga gtcatggttt atctgttggc
     1741 caaccaaaat tgatacaatc taacgatgca ccctctacgg cattctaggg gagtgcactt
     1801 gcattcaccc aataaaaacc ggcagttagc aaagaactta tgtagtcgta tgttcttact
     1861 gttaacatac aagaggtctc ctttccacaa ttgcttagca gtaaacggga atttacaaag
     1921 ctaatctctt ccattttctt atctttgttc taaaagtccc aaatggagca actgtattta
     1981 catgaataaa tttatatact tcccatacag ccgttttggt tgcctcgtct gcccattttc
     2041 ttttgtgtgc agtgaataga tcttcatcac tcattgaatc aatcatcatg ttaatatcat
     2101 caatattacg attgagaatt tcctctaatt ctttcaatgg tagatgtgca taggtatttg
     2161 taaaccactt atataactct cctaactgat tccatttaaa ttgatcagaa ggcgttttaa
     2221 cagataatcc atttctttca tcattttccc attttagaat taaattcgtc caaccaactt
     2281 gatatgctaa attttcagca ggtgttctat ccacttcctc aactctttta tccttcaatt
     2341 cttcaggaat agtttcaaat tctaaaatat atttagtata agtcctattt atttcttttt
     2401 ttaattcttc tttattttta tatgttctca ttatattacc tccataaatt cctaattgtc
     2461 tcctttaatt catctataat ttcatccacg atattttaat ccgttctaca atgcatatct
     2521 gatatgcatt gactgtctaa ctcatatgtg aatgatttat aaaatatccc aacgtctcat
     2581 ctaaagaaat gttgatcgtc agcaaggagc taaaaaatac gaatatattt cttttgtagt
     2641 aaaatcggac tggtttgcat atcattttct tcgtaaatat caaaatcatg ttaactacta
     2701 tcaattacta ataaaaacta ccactttaag cgttaagaac tgttaagttt agttaagtga
     2761 ggatagttca caaaatccat tgttaaactt cacgaaagtg gtaaaactca aaatgcttta
     2821 tataggatta cagcatcgct ctttcttcga ttgcgcgttg ggtaaaaaat attatgaagt
     2881 aaaaattgac gatgacacgt tccttacggc tcatcagatg aaacagctac aaaaacgaaa
     2941 tgatcaactc gaagaaaatt taattctaaa aaagcgattg ccatattcac gcctcgctcg
     3001 acgaactatt aaaggtcaag atttacctga agatcatcct ttttaggaaa ttgaagcttt
     3061 ttatgttgca caatgcattt ataatactac aatgatgttt tcaccagata ttcttctagg
     3121 gtgcggtgta atgaaacaga tacacttgca aaagaaaatt caccgcgaat ttactaaaat
     3181 tatgaataat tatgttaatc ttccgaaatt tttttccaaa attaagggat caagctggtg
     3241 ttatagattg tttaaggttg gcaaaaaaat aacttctttt taaacaatta tagaaaaccg
     3301 atactctgtt aagttggttg acacggtgtc gatttttatg actttcttat tctgaaactt
     3361 cactaacgga cagtacctaa aaagtctata gtatggacgt ttacgagtac tattttatca
     3421 aaaatgctgc tcatttcaac ttatatgttt tagtgaacta cccccactta gc
//
LOCUS       DACDIG010000139         3318 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.141, whole genome shotgun sequence.
ACCESSION   DACDIG010000139 DACDIG010000000
VERSION     DACDIG010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.141"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..2232)
                     /locus_tag="IV247_002810"
     CDS             complement(<1..2232)
                     /locus_tag="IV247_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778809.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="IV247_002811"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="IV247_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          907 a          744 c          536 g         1131 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg agtccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccata
//
LOCUS       DACDIG010000140         3311 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.142, whole genome shotgun sequence.
ACCESSION   DACDIG010000140 DACDIG010000000
VERSION     DACDIG010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.142"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(456..1766)
                     /locus_tag="IV247_002812"
     CDS             complement(456..1766)
                     /locus_tag="IV247_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP8778810.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1778..3196)
                     /locus_tag="IV247_002813"
     CDS             complement(1778..3196)
                     /locus_tag="IV247_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8778811.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
BASE COUNT         1017 a          673 c          513 g         1108 t
ORIGIN      
        1 gtttattgtc ttgaattgaa tcaagacgcc ctacacattt ggtttgtctt actttgttca
       61 gttttcaaag gtctacttca tcgagagtgt ttcccgattc atttttggct gccgtagcaa
      121 cttttatatc atatcagaga gacaaacgat tgtcaactct tttttaaaag tttttttcga
      181 tttcgttttt cgaaatcttt cagcaactca tttatcataa cttatcgttt gttatttgtc
      241 aagaactttt ttgaagtttt tttcgataga atcaagcttc gcaagtggtt ctttttaacg
      301 acttagttat cttatcacgt ctaaaaacat gtgtcaactc ttttttggaa ttttcataaa
      361 atgtttcaat gaagcgcgac aacgttgttt aatatatcat taaaaaaaat aaaggtcaag
      421 ctaaaatatt atttaagctt gaccttatta taatttcaac gattgtcaaa agtattgttt
      481 gcagctgtgt tgccagtttc tatagataaa tcacagcttt tgaaacataa gttttgtact
      541 gcttcgattc gcccgaattc ttttgcagtt atttggcaat gagaaaagca gacatcttct
      601 ataggctttt caggaaatgc tactaaatca aaagctcggg aaggaagtga gtaacctgga
      661 gaaaggcatg cctgtacgtt tttgatgtgt atgtctttga ctttcgtcat ttgctttgat
      721 tcaggtattt cttcagccac tgcttgccaa ctttctggca tttgagcaag gtcattgcta
      781 ttttttctat tgtattcatt atgccagtcc attatccaag aaaaaggaaa ttgtacatca
      841 atcatattca gattttctac cctgatattt tcgatcactc cccctctttc ttttgaagat
      901 ttcattcgaa taccacagtc tgactgaaaa aaatcgatat cgtgaatata tacgtcacta
      961 actccagcac taacttcgct accaatagtt acaccgtatc ctgaacggat tttacatcta
     1021 gcaacctcga ttcttgatga ttttcgattg actcttgcac cattgccatc tctgccagac
     1081 ttaatagcaa tacaatcatc accacaggcc aaatcacatt catagacacg cacatttgtt
     1141 gaagaatcaa tatcaatccc atctgtactt ggtccatcat tgttgcggat gattactttt
     1201 tctactaata catcgttaga ataagtgatt tgcaagttcc aaaaacctga tttctgaaaa
     1261 atcaaatcac gaacaacaac atgttctgca tgatacagta aacaagcgcg tggacgttta
     1321 atagcataat ccgcaatcca acgaagtccc ttttgatcgt attcagctct tgtcccgctt
     1381 ttttgatctt ttccccaata cagctcccac caatggtctc cttgaccgtc gattattcct
     1441 tttccttcta taagaatatc tttcgccgag agaacattca aaatcgctgc tggccatttc
     1501 atttctacgc cggctactcg tgtatcgatt tctggaaatg cttcgatatc tgttctcccc
     1561 ttgaggactg caccttcttc aaagataaga tgactcttat cttttaaaaa aagggctccg
     1621 actaaataaa tgccttttgg aataaataca ggtaagtgtt ctttcgcacc ttgatcgatt
     1681 gctttttgca gtattattgt atcatcttgt tgagtgtcgt ttgttttaaa gttcaaagca
     1741 ttgataatcc ctttttttgt ccgcaatacg tttctcccta tctttcattg atttttttca
     1801 attcttcacc aataaataca gtttttgaac gattcagtgg gtaacctact agcaaaatga
     1861 ttgcggatac aaataagcag aatgctggta acaagtttgc catcgtataa atcctttctt
     1921 gtactaaaac tgattggatg gctccacctg ttgtagacga ttggtaacca atgagtgcca
     1981 gcataaatcc gcctaccgct cctgcacatg cttgtcctac ttttctcgca aaagaattta
     2041 ctccatatac tgttccatct tctcttaagc ctgtcttata ttgatgataa tcaatcacgt
     2101 cagtaatgaa tgcccagacc atcaaattga ataaaccaga tcctaaagta gcgataaata
     2161 aaagaatcag atatacccat gcatttgtta tatgaaggaa atacatcagc aagtacatac
     2221 atgaagaagc aagtaacgca aatacgcttg cttccttttt accaaataat tttgtcagct
     2281 ttgtagcaaa tggtgctaaa actactactg ttccgtaagt aaataaaaga gcaacagaca
     2341 ttgcttgttt attatggaag taatcattga ataaataagt catattcgtc ccagctaaaa
     2401 tctgattgat cacaatgaaa atgtcaacaa tgactaaaat aatcagtgct cgattagcta
     2461 acaaaccttt taccaactga gaagcagata ctttttcgtt tttgacaact cttactcttt
     2521 cagtagtcaa tttacaagtt aacatatatg cgacaaatgc tattgcagca catccgattg
     2581 aaatccaaaa gaaatgattc cctgaaacga cttgttgtcc actcttgtta tgggtataca
     2641 ttaaaatcgg aatgaagaat cctgtagttg atccgccaat cgcagaacca atgcttcgga
     2701 acgtggacaa cgaggtgcgg tcattaggat ttgagctaat tgctgctgcc attgctccat
     2761 acgggatatt gattgttgag agaaaaatcc cataaacaat gtatgaaaca aaaatataag
     2821 caatcttcac cgtcattgga agatgttgga cgaatggcaa aaacaaaatg acagtcacta
     2881 agcaaaatgg atacttcatt tttctgatcc aaggaataaa tcggccagtt tctgttaact
     2941 ctgagacatc acataatctt ccaaccatca catcagcaaa cgcatccaaa attctcgaaa
     3001 tcaagaataa cgtacctacg attgcccctg aaacgccaag aacattcgta taataaatca
     3061 tcaagaaact attgactaaa cctaaaataa aacagttacc tagatcaccg aacatgtagc
     3121 ctatcttatc ggaaatccca aattcctttt tttctttttt tattgtattt gctgtaacag
     3181 cattttgtga aagcatttga attcctccta cctcttctag taaacgtttt caaaattata
     3241 actctttttt tcttgttttt atattcacaa tgttggcttt ttattgcatt atcttgacag
     3301 ataaaaaaca a
//
LOCUS       DACDIG010000141         3182 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.145, whole genome shotgun sequence.
ACCESSION   DACDIG010000141 DACDIG010000000
VERSION     DACDIG010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.145"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..632
                     /locus_tag="IV247_002814"
     CDS             <1..632
                     /locus_tag="IV247_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778812.1"
                     /translation="GYSKRLRITVEYGTNESATELKQRYLDTRREASKKYNDFKSRGL
                     IPKKKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYL
                     NTLLEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLR
                     ETQMYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            661..1779
                     /locus_tag="IV247_002815"
     CDS             661..1779
                     /locus_tag="IV247_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778813.1"
                     /translation="MGNNSIYTPEMQNKIKRLVGLGWMTRGKLDSFSTIKYLLMDIYR
                     QNKQTGIHFLGYDKPEDAPFFVPSSAYRASTSGSMYNLDLFEEGAKMIEYDFNEAYSR
                     IMRNYPLPSNTYLKAKYNTDQAILRAINRPKVRPYENLKTYMFFLVNFEGTAKEGTYA
                     NPKSQSMFADYGENPKTANEPIWLSEIELTLILDYYDIKEMEVLDSHTFLTKKGLLKE
                     YFERIEPLKTDPDTLKLYKNLRTKVFGAIGQKSLREGETSTFDKYSMYNRAYSSMVAG
                     VFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRSEVPEFEELYKLGVVKKKVHTISKEEV
                     EKALEFKNKGFENKGSFGNSKDEVKGVVIDDDFEFDFD"
     gene            1812..2132
                     /locus_tag="IV247_002816"
     CDS             1812..2132
                     /locus_tag="IV247_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778814.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            2387..>3182
                     /locus_tag="IV247_002817"
     CDS             2387..>3182
                     /locus_tag="IV247_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778815.1"
                     /translation="MRTHYIYLEDKIVDEIPAKSREQARKLFIEKYKEINGDSEVPKV
                     VVTVRKRQTKKRSTFHVSPDIYNLIERHRSEDNISISQYIATVIDELISGKYDYAFDF
                     EDEKDYKSVAVFQSDFEKLKNYAGRRNVNFARLLEEFYSEELGVGNEYKRETYAKLSH
                     KLSQALKEVTEVKAELDAKLEEVKKYQTERKNNMKNQVEFDEQKEFEKARKRYNKIMN
                     ELSYEHLTVHEELSEGTEKYTIADMVNEVETLLASFFENGHVNDEWR"
BASE COUNT         1228 a          389 c          694 g          871 t
ORIGIN      
        1 aaggctatag caaacgatta aggatcactg ttgaatatgg aacgaatgaa tcagctacag
       61 agctgaagca aaggtactta gatacacgaa gggaagcttc aaaaaaatat aatgatttca
      121 agagcagagg tctgattcct aaaaagaaaa agggaatttg gcgtgagttt ttcaaggaag
      181 aagatagcaa tcggaataag ggagacttca ttggacgata cacagcaaaa ggctacacta
      241 agaatttagg gatcaaggtt gaatacggaa ctggtgaaag ctcatgggaa ttgagaaatc
      301 gctatttaaa tacattgtta gagaaatata aagcagaatt attggagttg ttcgatgtta
      361 atgattttat cgagatggct gaaaagtatg gtcatgaaga aggttttatg atcaatgaca
      421 ttatggttat tgataatttt acggatttat tagcacttgc gaaaaaagca agggatttaa
      481 gagaaacgca gatgtatcta ttcagaaata aaaacggaag aaaccggaga actgaatcga
      541 gtgagattga agattactac gatgccttta cacacgatct gggagccttt gtagacgaga
      601 taagtggtgt aatgaacaaa tatagggtct agaataaaaa agccggagat gagcagataa
      661 atgggaaaca atagcatata cacacctgaa atgcagaata aaattaaaag acttgtcgga
      721 ttagggtgga tgacaagagg aaaattagat tcttttagta cgatcaaata tcttttgatg
      781 gacatttacc gtcagaacaa gcaaacagga atacattttt tgggttatga taagccggaa
      841 gatgcacctt tctttgttcc aagttcagcc tatagagcca gtacctccgg tagcatgtat
      901 aacttggatt tgtttgagga aggagcaaag atgatcgaat atgatttcaa cgaagcctat
      961 tcgaggatca tgagaaatta tcctttgccg tcaaacactt atttgaaagc aaaatataat
     1021 acagatcaag ctattttgag agcgataaat agaccaaaag taagaccgta tgaaaattta
     1081 aaaacgtata tgttcttttt ggttaatttt gaaggaacag ccaaagaagg aacatatgct
     1141 aatccaaaat cacagtcgat gtttgcggat tatggcgaaa atcccaaaac agctaatgag
     1201 cctatttggt tatcggagat tgagctaaca cttatccttg attattatga tattaaagag
     1261 atggaagtcc tagatagcca cacatttttg acgaaaaaag gattactcaa agaatatttt
     1321 gaaagaatcg agccattaaa aactgatcct gatacgttga agctttataa aaatcttaga
     1381 acgaaagtat ttggtgctat cggacaaaaa tcattaagag aaggagaaac aagcaccttc
     1441 gataaataca gtatgtataa tcgagcgtat tcctccatgg tagcaggcgt atttagagat
     1501 cgtatggtta gatatgaaca gaaatacgtt catagcgcat atgggctatt aaaaattcgg
     1561 actgatggat tgtatttcag atccgaagta ccagagtttg aagagctgta taaacttgga
     1621 gtagttaaga aaaaagttca tacaatatcc aaagaagaag tcgaaaaagc tttagaattt
     1681 aaaaacaaag gatttgaaaa taagggctcc tttgggaact ctaaagatga agttaagggt
     1741 gttgtgatag acgatgattt cgagtttgat tttgactaaa tactctttgg aaaagatggt
     1801 aggaggtaaa tatgaagaag aaaattgtgg aaggaaatgt aggtgttatt attctaaaag
     1861 attcaactga ctatgctaaa tcaatggcac taaaagacga aaaagttgca cgcatggata
     1921 aagatttagt tgaatttctg atggatgata cagaaagatt aaaaggggat cagtttttga
     1981 gtttaatgaa agacaaaggc ttaaacacat atggtttatt tgattataag aaattagcta
     2041 tagagtgggt aaaagaagga gaaatatttt ttattgaaga atgggaaggt actgagaact
     2101 tagttcttca tcagtatctt ttgaaagctt aatctaagta aaacagttct gactagttat
     2161 gcttgtcaga atttcaaagg atgttttcca taaccatcat cattcaaaaa taaaggattt
     2221 gaaaataagg gttcctttgg gaactctaaa gatgaagtta agggtgttgt gatagacgat
     2281 gatttcaagt ttgattttga ctagattttt ttgaaaggtt taagagtaaa caaggtaaga
     2341 actaagatca tgaagattga gagtaaagaa gatagaggac gaatgtatga gaacacacta
     2401 tatttattta gaagataaaa ttgtagatga aattccggca aaaagtcgag aacaagctag
     2461 aaaattattt attgaaaaat acaaagagat caatggagat agtgaagttc caaaagtggt
     2521 cgtaacagtt agaaaaaggc aaacaaaaaa gagaagtacg tttcatgtca gccctgatat
     2581 atataatctt atagagcgac atagaagtga agataatatt tctatctctc aatatattgc
     2641 tacagttatt gatgaattga tttcaggaaa gtatgactat gcttttgatt ttgaggatga
     2701 aaaggattac aaaagtgttg ctgtctttca atctgatttt gagaagctaa aaaactatgc
     2761 tggcaggaga aatgttaatt ttgcaagact actagaagag ttctactccg aagagttggg
     2821 agtaggaaat gaatacaaga gagaaactta tgcgaaattg tctcataagt tatcccaagc
     2881 gcttaaagaa gtgacagaag ttaaagcgga actagatgct aaacttgaag aagtgaaaaa
     2941 atatcaaaca gagaggaaga acaacatgaa gaatcaagtg gaatttgacg aacagaaaga
     3001 atttgaaaaa gcaagaaaac gctataacaa aattatgaat gaacttagct atgaacactt
     3061 aacagttcat gaagaattga gtgagggtac agaaaaatat acgatcgcag atatggttaa
     3121 tgaagtggaa actcttttag cttctttctt tgaaaatggt cacgtaaacg atgaatggcg
     3181 aa
//
LOCUS       DACDIG010000142         3090 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.146, whole genome shotgun sequence.
ACCESSION   DACDIG010000142 DACDIG010000000
VERSION     DACDIG010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.146"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(25..144)
                     /locus_tag="IV247_002818"
     CDS             complement(25..144)
                     /locus_tag="IV247_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP8778816.1"
                     /translation="MGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKGK"
     gene            complement(159..1343)
                     /locus_tag="IV247_002819"
     CDS             complement(159..1343)
                     /locus_tag="IV247_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP8778817.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(1602..1892)
                     /locus_tag="IV247_002820"
     CDS             complement(1602..1892)
                     /locus_tag="IV247_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778818.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(1882..3024)
                     /gene="dcm"
                     /locus_tag="IV247_002821"
                     /pseudo
     CDS             complement(1882..3024)
                     /gene="dcm"
                     /locus_tag="IV247_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
BASE COUNT          844 a          739 c          540 g          967 t
ORIGIN      
        1 aaattttgtc cgaaattcat cttgtcactt tcctttcttt tccattgctc gatccgctgc
       61 tgcacttgca ttcatcaagc acatcacagt aacaccaagc atactgccac caaataccaa
      121 aaaaatatct tttacaattc ccatgacgat tcctcttttc atataatcaa attttcaatt
      181 gccatacttt gttgctccaa aatcttttta tgtcggtctg ataagcgtgc attctgtacc
      241 atatcctgga tataggttgt ccgattcaat cgatccaact ccttagccat tttccacgta
      301 ggggcgactt gtcggcctag ccaattcaag gttcgttcaa tggtatacgg ttttggctcg
      361 gaagttaatt gaatctcttt ccgattccgt ccaataaaac gcccccattg ttcattcaag
      421 ggccagttgg ttcgcctttt accttctact ttatccgcaa atcgcaagta acgattgata
      481 atggaaaacg tggtgctctc aatgctacga ccttttaaca aatcttgaat cgcatgaaaa
      541 gcacggtcat ttttcagtcg aatctcaaaa cgatttttcg tctcagtttg gtctaatgga
      601 atccctaact tgatatattg ttcatagtct ttttcataca aacagaaata aacctcactc
      661 ttgagactgc cgatatataa ggtattgccc atgccgtcct tttcatcagc ctttaaaagt
      721 tccccggaac gataactttt aaaggtacga aacaaggaga tacattcttc cttctgacat
      781 tttttcgtta gctcaggtat atccaatagc cccactcgat cattgattgc caagtccaag
      841 cgtttcatca cgccaccggc ctttagacaa tcctcgaaaa agtcgtacca gctacgcttt
      901 tgagcgagta aaaaagtttc aaattgtcgg caacctcgcc cctttaattc taaaagcaca
      961 cctttatctt cttcatgaga caacatcacc acaatatcac ccatgacata ctgttcttga
     1021 taggagtaaa aggcataatc ttcatggagc atatactcaa atttcaaatg gagaatttct
     1081 tgaataattt ttcgtgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
     1141 gtcaaaggca aatcgggatt aaagctttcc agttggtcaa aaattttcct catggttttt
     1201 atagatgcgg ttacgccacc attttctatc tgatttaagt acggccgact aagtccacaa
     1261 gccgtagcca atttattttg tgaaacgccg tattcgttgc gtcggtcttt taatagccga
     1321 taatctaaat tccgttgtgc caagttctca cctccaaatt caaggtgtta actttttcaa
     1381 atgagttaac acctatacaa attacttact atctttgata caacaagcat tcttaatggt
     1441 tgcttactta ttttttgtgt ttttgtaccc ccctgttaga tacgggggtg agacatcatg
     1501 gtcggctaac gccgtccatg aacaggcaca gcaccacgga ggatttctgc ttcgcgaatc
     1561 ctccgcggtc agcgcctaac agtcagttgt gttggtttag gttactcatc tgtaatacca
     1621 tttacttcca actctgcttt catttctaac aagtattgac gaatatctgg ttcttttgct
     1681 tggtccgcaa tccgctcaaa ttctgcaatt acttgattta tttcaattcg agcatccgtc
     1741 tcctcattca ttaaatatgg atcgccagcg actttgacac cattcttgcg aatatcaaat
     1801 tttaaccgtc gcaattgttc ttctaattct gggtctgact gaatctttcc aatcacccaa
     1861 taaatttctc caatcaactc atcataaacc atgtacatcc aactctttta atcttctggc
     1921 aatatcaaaa atgaccggaa ctgttacaga gtttccggct tgcttataca actgactatc
     1981 tgaattgact tgagaatctc gatcaaaagc ccaatcggga aagccctgaa gtcgccaaca
     2041 ctctctaggt gttagcttcc gaatttgata ctgatccgtg acgatcgctt gttggtctcc
     2101 tgttagtaag gtatgaacac tctgttttcc cagacgacct cggcgacttg tggaattagg
     2161 aaaagccaga ttcacactgt cttctggctt cacctcttga tattctgttt cattttttcg
     2221 aatcatgatt tttgtaccac cttgtttcgt tgacaaggtg ggggcggttc cagtaacttc
     2281 ataaactcga ttaccttgtt caaactttcc cggtaatagt cctgctacag agattccatc
     2341 ccttgctggc agtgcgattt tgattccttc atcacctgtt aaggttggcg ctacgccatc
     2401 tgaaccatag acttgtccac ccattcctcg attggaagga ttgatattta ccaaatttct
     2461 gatgttttga tcagatgttg aagacggtcc aatgatagga aatacttgtt cggtacatgc
     2521 gtctcgagaa tgtccgataa ggaatatccg ctcccggctt tggggaatgt aggcctttga
     2581 gttaagcacc taccactcca cgtcataccc caatccatcc agcgttcgga ggatcgtctc
     2641 gaacgtagcc cctccttcgt gattgagcaa tcctcggacg ttctcaagga ataaaagctt
     2701 aggtcggaga agagtggtga accgagcgat ttcaaagaat aaagtacctc gagtatcgga
     2761 aaatctttgc cgctttcccg caattgaaaa agcttggcac ggaaatccgc cgcaaaggat
     2821 gtccactggt ccgagggatt gaataaactc atctgataca cttgtgatgt catgcatttc
     2881 cacctcctta cttgtatcat gaattgcttt ataactacgt ctagcaaatt tatcaatttc
     2941 acaaaaaccg atacaatgat ggctcgcctg ttccatacct aaacgaaagc caccaatacc
     3001 ggcgaataaa tctaaaaatc tcatacatta cctcattcat tgcaaagaaa aaagccaaga
     3061 acattttgct cttggctcat acattattaa
//
LOCUS       DACDIG010000143         3066 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.147, whole genome shotgun sequence.
ACCESSION   DACDIG010000143 DACDIG010000000
VERSION     DACDIG010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.147"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(285..899)
                     /locus_tag="IV247_002822"
     CDS             complement(285..899)
                     /locus_tag="IV247_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8778819.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1505..1882)
                     /locus_tag="IV247_002823"
     CDS             complement(1505..1882)
                     /locus_tag="IV247_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778820.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2182..3063)
                     /gene="galU"
                     /locus_tag="IV247_002824"
     CDS             complement(2182..3063)
                     /gene="galU"
                     /locus_tag="IV247_002824"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP8778821.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          926 a          642 c          473 g         1025 t
ORIGIN      
        1 gaaagaatcc atatggattc tttcttgtct ctttttccac tactttttat ttttctgaca
       61 ctacatattc ttataactca tttctcataa ctgtgaaaca acttataaaa ataacaaact
      121 ttaaacaaaa caagcttaaa atcaaagatg atccatctta aaataactaa aacaaaaaaa
      181 gtagtcgttg ttaatcctca ttaataatcg ttttcttttt ttcaaacgct ccatttttat
      241 gtggaacatt atttttttaa ggaactttgt caacatgtgg ataactattt tccacttgtg
      301 tcctggacta gctgttttgc tgtaaaatac aaaacttgct gcagcaaaaa gatcgttgca
      361 agagaggctg ttccgcagat cgctggatct agatttggca aagtcaagac tgcatcatgt
      421 aaattggcgt ggttgtccaa aaccaaatca gcaaattcaa ataatcgcaa accactgcta
      481 tgttgcgatt tgatacttcg tgacaaatca aatccagtaa caatgattaa tggatgacca
      541 ttttctttga tccactgagc tagttcgatg attgccgggt ttcttccttc attcgaaaga
      601 agaagaaaaa tgtcttctga tttgacagaa agctgatcaa taattgcctg tcctgcacct
      661 tctagcgttt catagacacc tccggctgga ttttcaatca atatacttgg tacaagatcc
      721 ggggtttgat cccaaaattc aaatgctaca ccacttagtg tgcggctagc atatatctgc
      781 aatctaccac cttgattaat ttgtttcgcg atccaagtgc cagcttgaag catcagcggc
      841 tcttctatcc ttaatttctc ttccaattgg tgataatagt caaaataaca taaactcatt
      901 tatctctccc gcctagctat acttgacatg aatagaaaca gctgcctaat ctaaggaagt
      961 acataaaaaa acagtagtta gacagtaaca catgtggtct ttgctgtttc aaacacaaaa
     1021 aaggcattga acgaatacgc ccaatacctt gacatagcaa ataaaacaac gtgcagacca
     1081 tggaaaatat ctacataggg gtagaatttt caccctagat taggtacaat aaattaggtc
     1141 gatgcactgc attgactatg gcaaaatgat cggtcagggt gtatgtgtgt gactggtcat
     1201 tccctgattc ttagcgccaa ctaagggtca gggagccttt ttttattcag ctgtttctat
     1261 ctgaatttat tgtaagtaat ctcttatcca agtgcaagca agaaaataca aaaaaacatt
     1321 ggtatgattt atatatatat aaataacata aaaaaaggaa tggcttatcc cgatacaaca
     1381 agcattcctt taaaaaagat tcttttatat aagtgaaaaa agcttttttc caccaattaa
     1441 ataaatctct catctcaata gtttatccat tattttatct ttttttgcgt atacctacta
     1501 aatattaccg tcttcttctc gttcttctac tttgttcgag agaatctgta ctagaaaaag
     1561 tagttgtatg tctacctttt ctgcctatcc gagactttct tttcatatat gggatataaa
     1621 tgattagata cagtacagca aaaaataaag caaatacgca agcttcccaa ctacctaacg
     1681 ccaattcaac catcaaggta gcaaccccaa cggatgctgc ataccagata aaaatcagaa
     1741 acgtttcttt ttttctgacc atgcgaaaca tgataaatag actaataagc aatagtggca
     1801 acacaaatat ccaattgctt ccttccatca gccacttgag tatatcaagc agcgactgtg
     1861 caacagcgac taattttccc atagtgaaca cacacctttt tttataatta aaaaattcgc
     1921 ctttttattc tactatgaat cttctggctt ggctttcatt tctcttatgt aatcattttc
     1981 ttttatacaa cttaaaaaaa aattaaacca ttagcataat attttctgaa tctattcacg
     2041 aaagtctttt tctatctttc gttttccacg actcaactga tctagagtca aaaaactaaa
     2101 acgacagttg cgcaaatgac gcacaagctg ccgctttaat aacaaatagc ctttcattct
     2161 cagcaggcca cttatttcca gttaactttc tgttcttagc tttttactca actggataat
     2221 atatgcactt aatgaagtcc caatttccga atgttgaagt ccatattcaa tatttgtctt
     2281 catatatcct aacttatcgc ctacgtcgta tctttttcct ttaaactctc tcgcatagac
     2341 cgcctctgtc tggttaagag tctgaagtgc atccgttagc tgtatctcgt ttcctgctcc
     2401 tggttcttga gtttctaata tctcaaatat tttaggagtc agtagataac gtccgataat
     2461 ggctaaatta cttggtgctt ttcccatttc aggtttttct acaaaccctt ttactctgca
     2521 acgacccttt tcgctatctg ttttaggatc aataattccg tatttagatg tttcattttc
     2581 tggtacacgc atgacggcaa cagcggaagc ttgtgtttct tcataatcca tcatcaattg
     2641 ttgagtcaat gggatatcat ctttcatcaa atcatccccc agcatgacga caaacggttc
     2701 atttcctata aatgcttttg cttgaagcac ggcatccccc agtcctcttg gatgagattg
     2761 acggatgaag tgaagattga cttgagtcgt ttcttcaact aaagcgagta attctgtttt
     2821 ttccttttcc cgcaaattgt tttccaattc gatatttgca tcaaagtgat cttcaatggg
     2881 tcttttttct tttcccgtta cgatcaagat gtcttcgatt cccgaatcca atgcttcttc
     2941 cacaataaac tggatcgttg gtttatccac gattggaagc atttcttttg ccatggcttt
     3001 tgttgccggc aaaaaacgtg ttcctagccc agcggcagga atgactgctt ttttgacctt
     3061 cacttg
//
LOCUS       DACDIG010000144         2993 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.148, whole genome shotgun sequence.
ACCESSION   DACDIG010000144 DACDIG010000000
VERSION     DACDIG010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.148"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(365..2000)
                     /locus_tag="IV247_002825"
                     /pseudo
     CDS             complement(365..2000)
                     /locus_tag="IV247_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2323..2802)
                     /gene="rlmH"
                     /locus_tag="IV247_002826"
     CDS             complement(2323..2802)
                     /gene="rlmH"
                     /locus_tag="IV247_002826"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAP8778822.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
BASE COUNT          951 a          552 c          459 g         1031 t
ORIGIN      
        1 tttctcagaa acaaaccact cattggaaaa gacatgaatc aaatcatcac ttacttgtgt
       61 aaaatagcga cgtttaatga caaagatatc ttcattgcta tctagtgtac cttcatttct
      121 ttgtgcttaa attcaaagat aattttctca ataaagattt ttgattccag taagattcct
      181 ttactccttt tacaacatag cgttgtcctt tgttttgaaa catcttcttc ccctctttgc
      241 cattaaaaat agcccaattc tctataaaga agattaagct tcattattat aatataaagt
      301 ttaaatttgg tttattactt actttttctt attctctagt aagctaatat tgaaaaaaat
      361 gtaatcagat cattattttt atatgttgta acttcctgat aagaaaacta attaaaagaa
      421 agaggtaact acataaacta attaaaaaag ctaacttcat agttggagga taaatggaag
      481 aaacttcaat gattgtttct ccttgtgttc cttcaatcga cactagtcct attatcaatc
      541 tctctttttt gtccatttac ttttgcgact gttccccaat aatcaaatac aggaagcgtt
      601 attttttgtc ctttagattc atgtactgta tagactattt tcgaatccat cacttgataa
      661 ggcatagtat gccattcctt gccaattctt acttcatgat tctgcaaatg ttctttgtat
      721 tgtaatgctt ccgtgggtgc atagtcataa agtcctccgg gaaggagtgt cgtgtaacga
      781 ataagatttt cttgattcat taccaagtga tctctctgag tgacaacttt atgttcatag
      841 tttgcttttg ttgcagcaga tacagccaac agtaataaaa caattgccgt tatattaact
      901 acttttcttt gcttagctga tgtgctcttc atgaaaacga acgataaaat caacgagcca
      961 aacagtactt gaaagcctaa gattcgccaa ggaaattgaa tcatttgaat gggtgtattt
     1021 tgtaataaag accatggaaa aaatttggtg gataagagcc aagagactac cgcactaagg
     1081 taaatcactt tttctttgcg attaaatcct ttaaaaaaga agagtggaca gatcaaagaa
     1141 ataataccaa tgattcctac cgtaaaggtt gtcaggtcat tattcatcgc accaatcaaa
     1201 ctatccgaga catttaatgc ttggtgttgt aaggtcgttt tatctggctc attaatcgat
     1261 tgaaccatga tttgtcggaa catcggatac caaaagtatg ctgttaaaag aatcgtcatt
     1321 cctactgcct ttgacaactc cacaatcaac cggatgcttt ctttttttct tgtttcactt
     1381 actacaaagc cgcctaagac tagtaagcca acggctatta cactcataaa agtggaaaga
     1441 acatgtgtat aaatgagtaa gctcatactg actgctaatg gaattgcaca tggctttttc
     1501 tcataaatca gtgcgtaagc ataatacata actatcggta taaccgtaag tgcaatcgct
     1561 tcagcaatag cagaacgatg ataaatatca atcgtacggt acccataaaa tgtatataaa
     1621 cagctaaata taaaagccgc atcgattctt tttaaaaatt ttagtccata atgaaacatc
     1681 agcatgatag tacaaacatt caatagccaa acatataaga tataactaac gattaaatta
     1741 ttacttatcc aataaaaaat gactgccgga aaaaaggtca agtagggata aaaataattc
     1801 actccgctcc cataatgatt aaacgtcgta aaattgattg gaccagaaaa aattgaagat
     1861 aaccctttga ctctagcaaa atggaaggca atatcccccg atgtagaaat agtatgaaca
     1921 tttctaaaat aattgattga ataaagtcca gcaataattg ctaggagaat ggacaatatt
     1981 atttttttct ttacttgcat tacttaatta tatactcctt tcatggattg ctttatctag
     2041 tataacttaa attttaaata aatcgttatt tatttttact ctcacttaaa aaagcactaa
     2101 aattataaga gagggaggta atggtctgaa caccaagaat tgttccaaca tcgaattgct
     2161 taactaatga gttcaaagct taattatgtg gctcttatat ttaccgagcg tggattgcga
     2221 tctctttagc gaaacaaaca tctttcataa aaaaaacagc catatccgct ttcttggata
     2281 tgactattta ctactacttt actatctttc attcttcttg tctcacttat gataaggcgc
     2341 ccccgcattg atccggaagg cacggtagat ttgctcgaca aggatcagac gcatcaattg
     2401 atgtggatac gtcatttttc caaaagagat ctgagcattg cttcttttca tgacggcttc
     2461 gctgagtcct aaagagccgc cgataacaaa aacaaggtgg ctttttcctt ggatacctaa
     2521 ttggtcgatt tgtttagcaa aagattcact tgtaggattt ttcccttcaa ttgctaaggc
     2581 atagacgtat tcttgttctt tgattttcgc taagatccgt tccccttctt tttcttttac
     2641 ttgtctcatt tgcgcttcgc ttaaattctc cggggctttt tcgtcaggaa cttcgatcaa
     2701 ttcgattttt gcataggcat tcagccgttt gacatactca ttgatacctt ggatcaggta
     2761 tttttctttt aattttccta ctgagataat tttgatattc atgttttctc tcctttggtc
     2821 ttcatgctca ctttgtttat tataatcaat tttttcgtct atatgtttat tttggctatt
     2881 tttgttaaaa agatttcccc aacagttatg cacaacatcc acaggtttat ccacagaaaa
     2941 cagtgttatt ttaccttatt tcatcacatt cattaggcaa acatacgttt tat
//
LOCUS       DACDIG010000145         2791 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.149, whole genome shotgun sequence.
ACCESSION   DACDIG010000145 DACDIG010000000
VERSION     DACDIG010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.149"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1350
                     /locus_tag="IV247_002827"
     CDS             <1..1350
                     /locus_tag="IV247_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8778823.1"
                     /translation="TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDT
                     VKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPALPEGTTVTDVTPGGT
                     IDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTDLNKEPDA
                     SEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVTVTDTRSD
                     ADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVT
                     YPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHG
                     TADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTD
                     NQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            1484..>2791
                     /locus_tag="IV247_002828"
     CDS             1484..>2791
                     /locus_tag="IV247_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP8778824.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFI"
BASE COUNT         1092 a          383 c          542 g          774 t
ORIGIN      
        1 actaacttgc cagcgttacc agaaggaaca acagtaacag atgttactcc cggcggtaca
       61 attgatacca acacaccagg taattacgaa ggtgtcattg aagtaacgta tccagacggt
      121 acgaaagata cggtgaaagt tccagtagaa gtgacagaca atcgttctga cgccgataaa
      181 tatgaaccaa cagtagaagg cgaaaaagta gaaattggag gcacagtaga tttaacagat
      241 aatgttacta acttgccagc gttaccagaa ggaacaacag taacagatgt tactcccggc
      301 ggtacaattg ataccaacac accaggcaat tatgaaggtg tcattgaagt aacgtatcca
      361 gacggtacga aagatacggt gaaagttcca gtagaagtga cagacaatcg ttctaatgca
      421 gataaataca atcctgaagg acaaaaagtg actacagact taaataaaga gccagatgca
      481 tctgagggaa ttaagaacaa agaagattta ccaaaaggaa ctacttatgc ttggaaagag
      541 aaagtagatg ttagcacagc tggaaataaa aaaggtacta ttgtagtgac atatccagat
      601 ggatcatctg atgaagttga agtagacgtt acagtgacag atactcgttc cgatgcagat
      661 aaatataatc ctgaaggaca aaaagtgact acagacttaa ataaagagcc agatgcatct
      721 gagggaatta agaacaaaga agatctacca aaaggaacta cttatacttg gaaagaaaaa
      781 gtagatgtta gtacagctgg aaacaaaaaa ggtactgtag tagtaacata tccagatgga
      841 tctaaagaag aagtagaagt tactatttct gtagtagata aaaaagcacc gaataaacct
      901 caagttgatc ctattacaga gggtgaccaa attgttactg gtaaaactga accaaatgca
      961 gaggtgacag ttacattacc agacggaagt caacaccatg ggacagctga taaaaatggt
     1021 aattttacag ttaaagttcc taaattagag gcagggacaa aagtgatagt aactgcaact
     1081 gatgaatctg gtaatactag tgaacctact aatgtagttg tttcttccaa caaaaaagac
     1141 agtggaaaaa acggtagtaa aggtagtaag actgacaatc aaggtagcaa cagcaatcaa
     1201 gacaaaaata gaggcaaaag ccagtcctca aaagtttttc ctaagacggg agagagtgac
     1261 agtaatatct ttactattag cggtggttta atactgttag gaacgttagg attattaggg
     1321 tataaaaatc gaaaaaaaga gaatgaatag aaaatcaatt tcttaagtct tctctgagaa
     1381 gaggaagaat ctttttcttc ctctttttgc ttttttatga tgggaaataa gtgttagtta
     1441 aagatgaata tactttttat agaaaggaaa agaatgtaat taagtggaaa aaatagtaat
     1501 tattggttca aaccattcag ggctagccgc agctcgattt ctaaacttaa gcgattcaaa
     1561 ttatgaagtt attctaatcg ataaaaaatc agaaattagt tacttggaat gtgggacatc
     1621 gttatttata aaaggagaag taagtaattt agaagatctt ttttatcaaa atagaatgac
     1681 actaaattct gagataaaaa agttatttct agagacagaa gttgacaaaa ttgatattca
     1741 aaataaaaag attagttgtg taaaaaaaga tggtacaaga ttctctcaag aatatgacaa
     1801 agttatatta gctacaggtt caaagcaaat tatgctagat ataccgggga ataatttagc
     1861 taatattttt tcgataaaga attttccaaa tgcaaaagag atttttaaag ctttggatga
     1921 tagtactatt aaaaaagtag gaattattgg agcaggatat attggagttg aaattgctga
     1981 agccataaga aaaagaggga aagaagtata tcttttcgat gttgcagata gagtgttgtc
     2041 gacttattac gaccgtagtt tttcagataa agttgaagaa atactatcaa aaaatggaat
     2101 acatctttgt ctatcagaaa gtcctaaaaa atatttagga agaaaaaaga ttgaaaaaat
     2161 agtcactaca agtggtaccc aatactctat ggatatggtc gtacaagcta taggatttct
     2221 atctaatagt ccaattgggg aaaaggaact cttgagagat gttagtggag catatctaac
     2281 taatgaatac cagcaaacaa atataaaaga tatatatgct attggggatt gtgctacaac
     2341 tttttttacg tccattaata gaagtagtgt tgatttttct atttctaatg ctctaagaac
     2401 agcttatatt gcaactcaac atattaacaa tcaagatttt acaccttctg gttcccaatt
     2461 tacaaatggc ttttctatat ttaattacaa tttttacgca gcaggtttga atttaaaaac
     2521 agcacgatta ttaagtgagt ctatagatta tatagaaata gaagatttca ttagacctgc
     2581 ttttttgaga aaaaatagtc gtgtaaaatt aagaattagc tttgaaaaaa atacaaagag
     2641 aatagtaggt gtacaacttt gtagtcaaga agatttatct ggactattat caatgttttc
     2701 tcttgctata gaagaacaaa ttacattaga caaattgaaa atgttagatt atttatttca
     2761 tcctagtttt agccaaccct ataattttat t
//
LOCUS       DACDIG010000146         2576 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.151, whole genome shotgun sequence.
ACCESSION   DACDIG010000146 DACDIG010000000
VERSION     DACDIG010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.151"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            190..>2576
                     /gene="esp"
                     /locus_tag="IV247_002829"
     CDS             190..>2576
                     /gene="esp"
                     /locus_tag="IV247_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAP8778825.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAGEGTSIESGTTVFAVKDGVS"
BASE COUNT          938 a          396 c          523 g          719 t
ORIGIN      
        1 tggttaaaaa gcacaaatat ccgaaaaaaa catgaagaaa tgtacttttt ttgttgtaat
       61 gttcatgaaa ttctctaagt aaattagtta acataaggtt agtaagattc ctattctgtg
      121 aataatacgt atcagattta tcttttattc ttactaggaa agtaattatt aagggagttg
      181 atttgaataa tggttagcaa gaataataag agagtatttc tagaaaaaac aaagaaacga
      241 gtattgaagt acagtatcaa aaaattaagt gttggggttg catctgtttt gattggtgtt
      301 ggtcttgtct tgggaacaac tgaactagtt aaagcacaag atgaaatttc tccaagcagc
      361 ccacttgaaa cagcgataag ctccgtacaa gtaggtgaca aagtagcttc ggggaatact
      421 tttcaggaga atcctggtta tacgaaaaat tataactttt cggatttaca atttagtcct
      481 caagaactaa ctggagacac actaaaaggc aatacaattg gatttgaagt ttacggaaaa
      541 cataatattg cagcatcaac taaaaactgg gaaatccgtc ttcaattaga tgaacgatta
      601 gctaaatatg tagaaaaaat tcaggtcgat ccaaagaaag gaattggatc ttctagacgg
      661 acctttgtaa gaattaatga ttcgctgggt agacctacaa atatttggaa ggtcaattat
      721 attcgtgcaa gtgatggatt atttgctggg gcagaaacaa ccgatacgca aaccgctcct
      781 aatggggtaa ttacgtttga aaagagccta gatgaaattt ttaaagagat aggcatagac
      841 aatctcaaga ccgatcgttt aatgtatcga atctacttgg taagtcatca agacgatgat
      901 aaaattgtac cgggtattga tagtacgggc tactttttaa cagattcaga tgatttctat
      961 aatagcttag atgtatctga aaataatcct gaccagttta aacatggatc agtaagtgca
     1021 aaatatgaga aacctaatac tcaaacaaaa gatggttcag gatccacagg tgctaatgga
     1081 gctataatct tagatcataa gttaacgaaa aattataatt tctcatattc atcttccgca
     1141 aagggcacgc cttggtatgc taattacaaa attgatgaac gattagttcc atatgtagcg
     1201 ggtatacaga tgcacatggt tcaggctgac aaagtaacat atgatgtttc gtttgaatcc
     1261 ggaacaaaag tagctgattt agcaatcgag cgacgggaag gtcatgaaaa ttatggcata
     1321 ggttcaataa ctaataatga tttaactaaa cttatcgatt ttgctaatgc aagtccacgt
     1381 ccagtcgtta ttcgatatgt tttacaattg actaagccat tagatgaaat cttagaagat
     1441 atgaaagcaa cagcacaagt tgaagaaaat aaaccatttg gtgaagattt catctttgat
     1501 tcttggttgt cggatacgaa taaaaaatta atccagaaca cttatggaac aggttattat
     1561 tatttgcaag atattgatgg tgatggaaac cctgacgata aagaagagag cggagacacg
     1621 aatccatata tcgggaaacc tgaattagaa gaagtatatg atgttgacac aacagttaag
     1681 gggaaagtat tcatccacga gttagcggga acaggtcaca aagcccaact tgttgataaa
     1741 gaaggtactg tattagcaga aaaaactatc gctccaaatg aaaaagatgg ggctccaatt
     1801 tcagatactg tagaatttga atttacgggt gtagattcaa gtaaattaat cgcgaaagat
     1861 gaattaaaaa tccaaatcgt ttctccaggt tttgataaac cagaagaagg ttcaaccgtt
     1921 attaaggaat caccaaaagc ggttgataaa caaaccgtgg tagttggatt taaaccagat
     1981 gctaaagaat caattcggaa taataaaaac ttacctgaag atgcagagta ttcatggaaa
     2041 acagagcctg atacttctaa cgttactgat agtacgaaag gtattgtaac tgttaagatc
     2101 ggaaatcgaa ctttcgacgt ggatgtagag tttgctgtaa aagcttctca agctatggaa
     2161 aatgatgcaa catacgtacc tataacaaca actccagaaa cgacagttca aagtggaaaa
     2221 cctacatttg ataaaccaga tgttccttta gctaaagatg ccttttcaat ttcagatgtc
     2281 tataataagt acttcggtaa tgcaagtgtt gatgcaaaca ctggtattgt tacattcact
     2341 ccagctaaag gtgtaggaga atcggagccg attactggaa caattcctat taaaattgtt
     2401 taccaagatg gttctgtagg cacgaccgat ttagcagtaa ctgtaagtaa agatatttat
     2461 gaaaatccag gagaaaacat tcctgcaggc taccacaaag taaccttcac cgcaggggaa
     2521 ggaacaagta ttgaaagtgg aacaacagtc tttgcagtga aagacggcgt aagctt
//
LOCUS       DACDIG010000147         2563 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.152, whole genome shotgun sequence.
ACCESSION   DACDIG010000147 DACDIG010000000
VERSION     DACDIG010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.152"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(1041..1706)
                     /locus_tag="IV247_002830"
     CDS             complement(1041..1706)
                     /locus_tag="IV247_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8778826.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDF
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            1832..>2563
                     /locus_tag="IV247_002831"
     CDS             1832..>2563
                     /locus_tag="IV247_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAP8778827.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERVISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKK"
BASE COUNT          828 a          405 c          458 g          872 t
ORIGIN      
        1 acatcaaggt tcctagcctt tttataataa aatgaagcga aaataatttc ttttaaagag
       61 ccgagattat cagctatata agagtgaatt tgatgcaaaa aacagagccg aaacagtctt
      121 ggtgttcgta gctctgctta tatgtttcga atttaagaca aagagttggc aaaagatgtt
      181 gataaggaag ataagataac tgtgttatat aaactaggta actaactgta acaagctaat
      241 tttaagagat tcttattaga agacgtttag gaatataact aagaaaatga ttatacaggt
      301 aattgtcatt tcaataatgt tttctaattt tcgcgtcttt gttttaagaa aaaggccaga
      361 atcagttgga aaaaagttcg tacgatagtg aagaggggca aaatttccta ctaatattcc
      421 cactaacaag agataaaacg gccaggttgg tgtgattcct ctataaaaaa atgcccatgc
      481 taccgaaatc ccatagatta acaactgaac aatttttaca atgaaaaaat tgttctgcca
      541 ttactcaaag tctcttcctc cttgtatcgt ttatttgttt cattttatca ttatgatagg
      601 cgcttgaaca atccattttg tattttattg agtactcttg gataaactac cagcgattat
      661 catatttatt tatgttactg taagccatca tataaaagta aatgagcaga tcatagaaat
      721 ataaaaaaga actaaaattg atctaaatta tgtgcagctt gactacgtac caatctatat
      781 tacttactag attggtcgtt aatctattcg gatcgaatga gcaaaatatt tttgtgaatg
      841 tccatccttg attatgattc caaaaagaat cattgggagg ctgtggcgta aatattatac
      901 tataagccag aaatacctat cattctgaac ttttttattt aggaactttc atctcaaggc
      961 tttttcaatt aatttgtacc cctgtttttt ggataagatc atgtgcattg attttttcta
     1021 tctctacgtt catctcctac ttaatgccta tctggctcct gagggcttct ttttaaggcg
     1081 tcgtcatagt cttggctgag gatttcttgg taatctgagg gaatggcttc ccaagtgagc
     1141 aggcgaacgt tcatgacgtg ttttcctttg tgtttgatta cagcatggtt tttctctttt
     1201 tctacctctc cagcgtgaaa agacagccat gtaccatggc caaagatatc attacatacc
     1261 caacgttcga tggaattgtc cacgtatcct acagggagtg tggtatagcc gatttgtggt
     1321 ttcaaaaaag gattgaatcg atcgatcgtt tcatttttgt attgataatt gataagaaaa
     1381 agatagacca aacaaaagac gagcagaaag aaagatagtg tctttttgag tgagaagggt
     1441 tgttttgatg aataagattt cttgttaaca agaggttcat ctggcaatgt tggttctatt
     1501 gattcggtag ctgattcaat agaaggaaaa tcaatcggca gacctaaagt atgatgtaaa
     1561 agtgacagca tttcaaagtc tggcattgta cgagaatttt cccaactaga aatcgtttta
     1621 tcggaaacac caattttttc gcctaattct ttttgcgtaa gcttacaatt tctgcggtaa
     1681 gttcgtattt tttctccggt gttcatcttc ttctcctttc gattctacag tatatagatt
     1741 tattctacat gacctataca cttgatagat acgttttttt gatgaaaaac ggtatgattt
     1801 tatcaaagaa tcatttaagt aagggagagt ggtgataatc aaaaaaaagt tcacagtttt
     1861 actcacaatc atagtgaccg gtatcatgag tggttttgcc ttggttagta taaataaaca
     1921 ggtactctat cggacaaatg aatttttcca tcaaacagat acttatactc cgattcgatt
     1981 gccacaacat tttattgggc aagaagaata tgagcagcta atcatggtat ttgaaaatgt
     2041 ctcatcagaa actgagcttt cttatatgaa aaaaacagta aatgaatcca atgaagtaga
     2101 tacatcttgg aacttcaaga tacagccaaa gcttgaaatg acattgtatt atcaagggga
     2161 tgtcaaatct tctcacgaga aggtacccta ttctaatacc tcctttcgcg taacgtccat
     2221 tgaagaatcg atcaaaataa aagattttga aggtgaattt ttcttgatga cgagagaaaa
     2281 agaagcctat gaacgggtca tttcactatt cactgatcga ttcaatcaag catttcacac
     2341 tgattatgcc actaatattt ttgaagactt tgaaaatagc tattcgtatt cggttatttc
     2401 aaatacagac gtatataatt atcgagtgta tttaactatt ggattgattc ttttattcct
     2461 tttgttatca tcctttttct tattgatgaa taaagaaatc agtttattgt tgaataatga
     2521 cttttcatta catcaagtat tgtggtgttt attggggaaa aag
//
LOCUS       DACDIG010000148         2556 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.153, whole genome shotgun sequence.
ACCESSION   DACDIG010000148 DACDIG010000000
VERSION     DACDIG010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.153"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..809
                     /locus_tag="IV247_002832"
     CDS             <1..809
                     /locus_tag="IV247_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAP8778828.1"
                     /translation="SGKGYFQNNSTQEHDFQIKSSVQVGNKADSESKEFLGIRYDPNK
                     YPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYADLDQLGRAGNVEALITYDAVNS
                     HSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKDSNNEITQLEGYKGYLYNKSHS
                     LAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSETIVRDAIYKNKNEKVLYKVDPI
                     YKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKGININYQNGTWVRD"
     gene            829..1155
                     /locus_tag="IV247_002833"
     CDS             829..1155
                     /locus_tag="IV247_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778829.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMIIEREMLDEIRVIIPKDTQMCE
                     DSISDD"
     gene            1189..2451
                     /locus_tag="IV247_002834"
     CDS             1189..2451
                     /locus_tag="IV247_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778830.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVANFSKSVYLVGRALVVSAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATRGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETEKSTASIAVSNSQFAFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
BASE COUNT          995 a          361 c          519 g          681 t
ORIGIN      
        1 ttagtggcaa gggttacttt cagaataaca gcactcagga acatgatttt cagatcaaaa
       61 gtagtgttca ggtaggtaat aaagcagaca gtgaatcaaa agaattttta gggatacgtt
      121 atgatccaaa caaatatcca aataatatcg taaacattaa tgatgggaaa tatacactga
      181 caaaagaaga ccatgaagca ttatcagaaa aaggcagtca aaccttttgg gtaaattatg
      241 cagatttaga tcaacttgga agagcaggaa atgttgaagc gttgatcacc tatgatgctg
      301 taaattctca ctcatctaaa aatgtggaac gtccatcttt caaaagttct gtccatattg
      361 caggagaata caaggatgga gtatttgatc aagagagaga tacttgggtc ggcaaagatt
      421 caaacaatga aatcactcag ctagaaggct ataaaggtta tctctataac aaaagtcatt
      481 cattagcatg gtcgctagga ggagatatgg aggtacacaa tttaacactt ggaacgagag
      541 cgcaaaatgt tgggaaaaat gatcagaatg gagggatggc ttactcagaa acgattgtac
      601 gtgatgcaat ctataaaaat aagaatgaaa aagtattgta taaagttgat ccaatttata
      661 aagggaatga attggttcct cgtggttctc atgtcgaagc tttttcagtt aacgataatg
      721 gaaaaacggt aaaccttaat gtttatgtat ttaacaatca aaaaggaatc aacatcaact
      781 atcaaaatgg tacttgggta agagattaga aattgagaag gtgaagaatt ggaaacaata
      841 agcatcaatg gattagataa atcagttgca aatcgaataa aaaaaggaaa tggctactat
      901 cttctgaaga agggtaaata ttcagttgct agagagttag aaacaatgag tatcgtgtat
      961 gtagctaaga atcattttga cgtagaacag gctatttttg atccagattc tgaaataggg
     1021 aaatgtgttg aattatcaga gaaaactgtt agtacaaaag gggcgatgat cattgagaga
     1081 gaaatgttag acgaaatcag agtaatcatt cctaaagata cgcagatgtg tgaggacagc
     1141 ataagtgacg attaaccaaa atcagaaaga aaataaggag atattcagat gagcgaaaaa
     1201 tccattgaat taaaggaata taagcgaagc gatggagaaa aatattatcc tgtagccaat
     1261 ttttctaaat cagtatatct ggttggacgt gcattggtag ttagtgcaat tggcatatat
     1321 attttgcatt gggtaacaaa ggttgtacaa ctgcttgtag ataccttagt aagtattctc
     1381 gtctcgttaa atgatgttaa cggaacagat gatttaggat taaaagagat gtttaaagag
     1441 ttactcaatg cgttttttcc aaattattta agttattcat ataatgtaca aagctttttt
     1501 gcaaagctac acttggtaga tatctctatt ttagtattag aagcaggaac ggtattcttt
     1561 gtcggatatt tggttacttt gatgttcagg catcactatg gagaacaaag accattcttt
     1621 gatgatatgg aaagtagaag gttgaaaaga gaagctctag aagctatgga tgtgaagtat
     1681 gatccaaaca agattatagg agatgaaacg aacaacaaaa aaagaaaacg aaacaaaacg
     1741 caattagaat ccgatgtgct atcaacgctt aaaaatatca aaatttctgt tcatacaaga
     1801 cgtgatctaa gtgtcaatga agatattcga gaatatatcg taaaaatacc gcaaccaaaa
     1861 gggaagaaag ttagagaaac agttttacga caaattcaag atttagggtc gatcctgaca
     1921 agagcaacaa gagggcgagc taaatttggg gaaaaagaag acgaagtttc aggtgattac
     1981 ttcatcttca gagcatcagt aaacattgaa agagaacgta aggaaagagt aagtaccaaa
     2041 aacaaggaaa cggaaaaatc aacagcatcc attgcagttt caaattcaca atttgctttt
     2101 ccaatcaatc tctttgcaga taactccaaa aatatccaaa accaaaccga aaaagctaga
     2161 aaatatgccg aaactcaaaa gcaatcttta gaactttatc tatcttctgc tgacgtacct
     2221 gcaagtctaa cagatatgca cgttgggaat acatctattg aatatttgtt tactttatct
     2281 cgtaccagaa aaattcaact aagctcaatg aaagaagaaa ttgaaactta cttgcatata
     2341 tcaggtgtaa tcgtgcggat caacgcaggg cgtattcaaa ttactttacc attaggagaa
     2401 aatgatcgta ttccaattga tgtaccatca atgatcaata gcgtgtttta gaagacatca
     2461 caaatagaaa cagaaaatac aaactaccag ttcacaaaca gaaaagtacc gagaaaagag
     2521 aggaatagaa atggctatga aattgttaga gaaagt
//
LOCUS       DACDIG010000149         2548 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.154, whole genome shotgun sequence.
ACCESSION   DACDIG010000149 DACDIG010000000
VERSION     DACDIG010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.154"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..238
                     /locus_tag="IV247_002835"
     CDS             <1..238
                     /locus_tag="IV247_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778831.1"
                     /translation="EQIALEYVFTNEEGEEIEKVLIISSKDLEKALAYEPYESTVTVI
                     EFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            320..505
                     /locus_tag="IV247_002836"
     CDS             320..505
                     /locus_tag="IV247_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HAP8778832.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            572..967
                     /locus_tag="IV247_002837"
     CDS             572..967
                     /locus_tag="IV247_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAP8778833.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            957..1334
                     /locus_tag="IV247_002838"
     CDS             957..1334
                     /locus_tag="IV247_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778834.1"
                     /translation="MKSKKETEPMKATHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            1335..1730
                     /locus_tag="IV247_002839"
     CDS             1335..1730
                     /locus_tag="IV247_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778835.1"
                     /translation="MKQEKRKMKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLW
                     KYKILFLFWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVIS
                     DAGIERNIKRVSFKITLNKPTDKKLSEAF"
     gene            complement(2037..2435)
                     /locus_tag="IV247_002840"
     CDS             complement(2037..2435)
                     /locus_tag="IV247_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAP8778836.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
BASE COUNT          913 a          455 c          402 g          778 t
ORIGIN      
        1 agaacaaatc gctttggaat acgttttcac aaatgaagaa ggggaggaaa ttgaaaaagt
       61 cttgatcatt tcatcaaaag acttggagaa ggctttggct tatgaaccat acgaatcaac
      121 cgtaactgtc attgaattct tagataccta tacttctaat gatacttggg aaattatgaa
      181 tatttgcaac gctaataaca ttcaatttgc tgaaaaaacc aataaatcat tttattagtt
      241 ttgcaataaa acatcctctt tttctattga ttccatacac aacaatgtgt ataatagatt
      301 ttgtaaaggg agggtagcta tgcctaagac agcaagagaa attgtaaaaa ttctcaaatc
      361 taacggcttc aaaaagactg gacaaactgg cagtcatgca aaattttata attccaagac
      421 taaaagaacg gtcgttgttc cgatccacaa tggtgatgta ccactaggaa cagaagctaa
      481 tatctggaag caagcagggc tttaacagcc ttgccttcct atttggtatt aggcatacta
      541 tcctctctta cactagaaaa ggagcgagga aatgaagaca aaaaatattt tttatccggc
      601 aatcttcgag aaagaaggag aagcttataa catcgttttc cctgacttac cggaaatatc
      661 tacctttgga aatagtcttg aagaagcata tttaaacgct aaggatgctt taggattagc
      721 gctatatagt gtaccagatg cagaatttcc taaaccatca gcagtggaag aattaacttt
      781 gaaagccaac caagtggtag taatcatcca actaaacatt aaacttttcc gaagaagctt
      841 aaatacaaaa acaattagaa aaaatgttag tgttccggaa tggttagttc ttttagggaa
      901 ggagaaaaac ataaacttct ctcaactact tcaaaaagca ctggaagaag agttgcttga
      961 aaagtaaaaa ggagaccgaa cctatgaaag ctacacactt aaactatttc tctcgaacgt
     1021 taatcaataa tctattaaaa gatacaactg gcattagcgt tagtaccaaa cactttgcca
     1081 atgatctaat tatttttgag gataataact tgcgaattga gatgtttatt gaacaggcaa
     1141 cccctaactc aatacctgaa ttctgctata cgcacactga aaaaatgaaa catgcaaaat
     1201 cagttaaaga tgttgaagca ttaagtgaat cactaacgaa cgctttaaac ctcgttaaga
     1261 aggcaaaatt gttatttgaa tttcaaaccg actaccacaa tgagttgctt gctcaatctg
     1321 aaattttaga ttaattgaaa caggagaaga gaaagatgaa acaaacacaa acagaaaccg
     1381 ttaacgatac gcttgctgat caattagcag gttattataa attatttgag atgggtaaag
     1441 cacagcgctt gatccactat ctttggaaat acaaaatttt gtttcttttt tgggaatcaa
     1501 cactaacatt ctcagttctt ttttataagt cagattttta taatcaacac ttagttttag
     1561 ggagtttttt gcttatctac accattttga tgcttaattt atatacctac atcacacttg
     1621 aagaacactt agtaattagc gatgcaggga tcgagagaaa catcaaaagg gtatccttca
     1681 agatcacgtt aaacaagcct accgataaaa agctctcaga agcgttctga gagcttttga
     1741 cattttcttg tataatttta tcctaacaca ttacaaagca cctaattcga gcttatatca
     1801 agcaaatttg ctatttatta ctcaatacat atctaaaaat tacttgatgg aaaattagat
     1861 acatggtaag catgaaaaga aaatcaattt gagcagatgc agtacaatac atagcaattt
     1921 aaaagctctc agaaggcttc tgagagcttt taatatattc tttgataatt tgtctttctt
     1981 aaatccatac gtcctaaaac aagctgtttt tgcttaatat gatcatctga gagacatcaa
     2041 atcaactttt ctctcaacaa atcattcaaa gctttcgaca gattaatatt ctcttcagcc
     2101 gctaaaacat ttaaatagtg ttccacagtc actgattttc gatcataatt ttgcttgttt
     2161 cgagctttag caactttcgt attagctttt acgacaaata aattaacatt ctcagatcgc
     2221 ttgattcttg catttaactc ttgaacaggc gtaggcgatg gaatttcttt ttcttgcaac
     2281 tctaaaagca ccaagtatcc ttccaatact tcttgcgcca tctcaaaagc atcttctaaa
     2341 gtttcaccat gtgtatataa gtctggaaaa tctgggaatt taactgcata ttcatttcct
     2401 ttccattcga atactgcaca aaaaaagtat ttcatcgcat tgcacctctc taattaaaaa
     2461 ataatttttg cttttttgtt tcaaaaaaaa gaatcgccta ccaaaaaaat tgataggcga
     2521 tcacaagcta attaacctct aaaggaga
//
LOCUS       DACDIG010000150         2497 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.155, whole genome shotgun sequence.
ACCESSION   DACDIG010000150 DACDIG010000000
VERSION     DACDIG010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.155"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(526..1866)
                     /gene="glnA"
                     /locus_tag="IV247_002841"
     CDS             complement(526..1866)
                     /gene="glnA"
                     /locus_tag="IV247_002841"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAP8778837.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(1894..2286)
                     /locus_tag="IV247_002842"
     CDS             complement(1894..2286)
                     /locus_tag="IV247_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8778838.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT          720 a          550 c          418 g          809 t
ORIGIN      
        1 tcgtgcctcc ttatttctaa ataaacggtg ttctcctgcc aactcctcgg tcacaagtca
       61 caatatccat caaacatgag aagctgtttt tgaaattatt tcttgtgatt cggagttaaa
      121 tggtaggctc acaacctctg gataaacggt attcaaaagc cagtttctcg gtattaagcc
      181 aaatttatgc aaaaatgtag aaagccattt tcacaaaatt tttcttaata ctcgaaacta
      241 aacgtctttt tcaaaacctc ttggtaaacg gtgttcaaaa gcttgcatct gcggtaataa
      301 gttcagccaa ttcaaaaaca agaaaagctg tttttgcttg gctattctta ttacttgttg
      361 cagggcagct ttttcacaac ctctccacta aacggtgttc aatcagctga ccctcggtat
      421 tagctcaaaa aacggcaaaa tatgaggaac tatttttccg ttagtttctc taatactcag
      481 gtcaaaacgc tgatttcaca gcctctaatt cacggtattc ctgttttaat aaagttctag
      541 atattgttct ctttcccact cggaaacagt ttgtctgaat gccgcccatt cgagacgttt
      601 tgcttcaaca aaatttgaga agatatgttg tcctaatgcg tcaatcatta cattatcctt
      661 acgcaattct ttgatagcat tatggatagt tgaaggtaag tcctggatat gtgcagcttc
      721 tctttcttcc tcatccatta cataaatgtt acggtcaaca gctgctggcg gtgtgatctg
      781 attacggata ccgtctagtc ctgcttgcaa caatactgcc attgccaagt atgggtttgc
      841 agatggatca actgaccgca attccaaacg tgtggataaa ccgcgagatt ctggtacacg
      901 tactaatggc gaacgattac ggccactcca tgcgacataa acaggcgctt cataacctgg
      961 aacgagacgt ttataagaat tgactgttgg attgcaaacg gctgtgtagg cacgagcatg
     1021 tttcagcaaa ccacctaaga aatgataagc tgtttcactt agttccaatt caccgtcttt
     1081 atcatagaaa acatttcctt catcattgaa caaagacata ttgcaatgca taccagaacc
     1141 attgatccca aataacggtt tcggcatgaa agtcgcgtga agtccatgct tgcgtgcaat
     1201 cgttttgaca acgagcttga aggtttggat attatcacaa gcatctacca catctgcata
     1261 tttaaagtca atttcatgct gtcctggcgc tacttcatga tgtgaagctt ccacttcaaa
     1321 gcctagactt tctaattcca acacgatgtc acggcggcaa ttttcaccta aatctgttgg
     1381 tgcaaaatca aaataaccac ctttatcatt caaggtcgtt gtgatcccac cgtcttcgtc
     1441 taatttaaat aagaagaatt ctggttcagg tcctaaattg aaagaggtaa aaccaaggtc
     1501 gcgcatatct ttcaatgctc gtttcaaatt cccgcgagga tcgccggcaa atggtgtccc
     1561 atccggatta tagatatcac aaattaaacg agcaactttt ccatgagcac tttcccacgg
     1621 gaagatcatc caagtggaga gatctggata caaatacata tcgctttctt caattcgaac
     1681 aaatccttca atcgaagaac cgtcaaacat cattttattt tctaatactt tgtcgatctg
     1741 gctcactgga acttctacat ttttgatcgt acctaagatg tctgtgaaca tcaatcgtaa
     1801 aaatcgtacg ttttcttcct caatgattcg tttgatttct tcacctgtta tctgtttctt
     1861 taccatggta tccctcttcc ttttttaatt tattcagtca aagaattgtc tttgcaatcc
     1921 ttgcgactga taatgacctg ttttgcttaa cccaccttgc gccaaaatct catcacgaaa
     1981 aatttgtcga acgtcatgat ctgtcaacgg cttcttgttt tttgcttctt gttttttcgc
     2041 ctgtgccatt tcgtatacac gtttgatccc agccatattc aatccctctg aaagatagtc
     2101 tttaatctca agcaacatat caatatcatt cagcgaatac atacgtcggt ttccttcgct
     2161 tcgttccgga tgaactaaat cttgttcttc ataatagcga atttgccgag cagacaaatc
     2221 agtcagcttc ataactgtac caattggaaa tacggacata gatcggcgca attccttttc
     2281 tcccatcaga cgtccccttt cgttttataa gttggctaaa gtgtaccaat taaaaaaaat
     2341 attgtcaact gcatatgtta ggttttctaa catgagatct ctatttttta taaaataaca
     2401 aaaaaataag aacacaaaaa aggtatctag ttgattttcg taccagatac cttttatttt
     2461 ttattatact gtactatcct ttattagtgt ttttact
//
LOCUS       DACDIG010000151         2468 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.156, whole genome shotgun sequence.
ACCESSION   DACDIG010000151 DACDIG010000000
VERSION     DACDIG010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.156"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..238
                     /locus_tag="IV247_002843"
     CDS             <1..238
                     /locus_tag="IV247_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778839.1"
                     /translation="TSISRVENSIGTNTNSASTNSSYEATPTTEFKVGKDVVISIDGK
                     TTKAEVISIDGEKAVVKTPDGNQYKIQAHLSDNN"
     gene            250..483
                     /locus_tag="IV247_002844"
     CDS             250..483
                     /locus_tag="IV247_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8778840.1"
                     /translation="MREEKIVYPVIQNNQENMTSSQHLQEAQLVYQVNELPQTNETMS
                     KSISLIGLLIVAIVITVGVYRKLNVKGSISDGR"
     gene            473..1339
                     /locus_tag="IV247_002845"
     CDS             473..1339
                     /locus_tag="IV247_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778841.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVEWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLKFIFVPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNSARVSMGKKKPFRGTEILKVKQDGTIVYRDGSVALMFNLDGATSLTAFP
                     EEVMAQESKSIQYQNTRDRSLAEIKITSSQPQNTERQTRNMKIQKRNARSNKAVFDII
                     DQQERFVSEYIDGVKTTVIQYLILKSQSKMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSDIYALK"
     gene            1354..>2468
                     /locus_tag="IV247_002846"
     CDS             1354..>2468
                     /locus_tag="IV247_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778842.1"
                     /translation="MEIMGRSQLLNFKKKRKTKKEQKTRNSKVVNNTFVSDLTSLVGY
                     QFTPSYIRTGKRWGTIVKLVNTYGMNRNYGFGWMVKAIPEVRVEGVKAYLIEADKPVS
                     QKDQQEIMHENVGGSTNSYENNLEGKNSDAEISKTVMRIMDLNTASLLDSMANKIIDW
                     KLEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSYGGDQRDLFDRLLSEPIGNVYE
                     YTSMSSDFAGNDHAIRKGLDDANGWAIGTMSTSYASGVAFMALDDSFTSKVLVASHKN
                     ATIKGYDEKISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGRTFVAKPSLNS
                     VIEKVNLAEEGLNSLELFGNQKDIGDLYRRNLDKLTQ"
BASE COUNT          908 a          355 c          489 g          716 t
ORIGIN      
        1 aacatctatt tcgagagtgg aaaattcgat tgggacaaat acgaactctg cttcaacaaa
       61 tagtagctat gaagcaacac caactacaga gttcaaagtt gggaaagatg ttgtcatcag
      121 tattgatggg aaaacaacta aggctgaagt gatttctatt gatggagaaa aagcagttgt
      181 gaaaactcca gacggaaatc agtacaaaat tcaagctcac ttatctgata ataactaagg
      241 agggctatta tgcgagaaga aaaaatagtt tatccagtaa ttcaaaacaa tcaagaaaac
      301 atgacctctt ctcagcactt acaggaagca cagctcgtgt atcaggtaaa tgaactacca
      361 caaacgaacg agacaatgag caagtcaata tctttaattg gcttgcttat tgttgcaata
      421 gtgataactg taggtgttta ccgtaagttg aacgtgaaag gaagtatttc tgatggcaga
      481 taactcaaac tatggattta aacataactt acatcaaaaa ccttctgaaa caattgtcag
      541 agttgaatgg cttgaaaagc atgtcaatag cacctattct gttacagcaa aaagattaat
      601 tgtatcagct atagttgttg cagtcgttgt tcttggatca attgtttact tagtttctga
      661 tacgattaaa ctgaaattta tttttgttcc aacgttatta atgataattg gatttcttat
      721 tctaattagc cagtatatgt ttattgatcc atctagtggt tcattgattt ctggttctat
      781 tagactgttc aaatcagtag ttaattcagc aagagtatct atggggaaaa agaaaccatt
      841 tcgaggcaca gagattttaa aagtgaaaca agatggaaca atcgtatatc gagatgggag
      901 tgtagcatta atgttcaatc tagatggggc tacatcacta accgcttttc cagaagaagt
      961 tatggctcag gaatcgaaat caatacaata tcaaaatacc agagatcgtt ccttagctga
     1021 aatcaaaatc acttcatctc aacctcagaa tacggaaaga caaactcgaa atatgaaaat
     1081 acaaaaacga aatgcaagga gtaacaaagc agtctttgac ataatagatc agcaagaacg
     1141 atttgtatca gaatatattg atggtgtgaa aacaacagta atccaatatc ttatcttgaa
     1201 atctcaatct aagatggtat tggatgaagg tgtggaacgt ttagaaaact atacgagtag
     1261 aggactttat tatagtgtca ctcagttgtc agctaaacaa acaaaagaaa tattatctga
     1321 tatttatgca ctgaaataga ggtgagagat taaatggaaa ttatgggtag atcacaacta
     1381 ttaaatttca aaaaaaagag aaaaactaaa aaagaacaaa aaactagaaa ttcaaaggta
     1441 gtgaataata cctttgttag tgatttaaca tctttagttg gttatcaatt tacaccatct
     1501 tacattagga caggtaagcg gtggggaaca attgtgaaat tagtgaatac ttatggaatg
     1561 aatagaaatt atggatttgg ttggatggtt aaagctattc ctgaagtgag agtagaaggg
     1621 gtaaaagcct atctaattga agcagataag ccagtgtcac aaaaagatca acaagaaata
     1681 atgcacgaga atgttggggg atcaacgaat agttatgaaa ataatttaga aggaaaaaat
     1741 tcagatgctg aaattagcaa aactgttatg cggattatgg atttgaatac agcatcttta
     1801 ctagacagca tggcaaataa aattattgat tggaagctag aaatcctatt agtatccgat
     1861 tctcctaata aaatcagcga acagctaatt aaactccaac ggttgtatga tgaaaaaatg
     1921 tatggattaa agcttatgtc atatggagga gatcaacgtg atctgtttga tagactttta
     1981 agtgaaccaa tcgggaatgt ctatgagtat acgtctatga gttcggattt tgcaggaaat
     2041 gatcatgcaa ttcgcaaagg tttagatgat gcgaatggat gggctatcgg cactatgtct
     2101 actagctacg caagtggtgt agcttttatg gcactggatg attcttttac aagtaaagtt
     2161 ttagtggcat cacacaaaaa cgctacaatt aaaggctatg atgaaaaaat ctcagcatca
     2221 tcactttggg ggcaattgat tgctaataat gcaatggcaa atcataaacg aacttttcat
     2281 attgtcttaa ataaatttga ttactttggt gatccaactg gtagaacatt tgttgccaaa
     2341 ccttcgttaa acagtgtaat agaaaaggtt aatttggctg aagaaggtct taactctttg
     2401 gagctttttg gtaatcaaaa ggacattggt gatttatata gacggaatct cgataaacta
     2461 actcagat
//
LOCUS       DACDIG010000152         2458 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.157, whole genome shotgun sequence.
ACCESSION   DACDIG010000152 DACDIG010000000
VERSION     DACDIG010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.157"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            5..1149
                     /locus_tag="IV247_002847"
                     /pseudo
     CDS             5..1149
                     /locus_tag="IV247_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
     gene            1320..1778
                     /locus_tag="IV247_002848"
     CDS             1320..1778
                     /locus_tag="IV247_002848"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAP8778843.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1937..2407
                     /locus_tag="IV247_002849"
     CDS             1937..2407
                     /locus_tag="IV247_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAP8778844.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          823 a          460 c          459 g          716 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgtcagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccaa aagatctagc cgatcagatc
      661 ggattttacc aaaatgctat cggagccgta cttggtcccc aagatagctg gttacttcag
      721 cgcggaatca agacactaag tgttcgtatg gaggaacatc aaaaaaatgc ttttgtggta
      781 gctgattttc tattttctca tccagcagta gaaaaggtct actatccagg attacccgat
      841 catgaactcc atggagttgc taagcagcag atgagcggct ttagcggaat gatttctttt
      901 actttaaaaa atgaagaatc tgctattcca tttgttgaat cactccaact atttacttta
      961 gcagaaagtt taggtggcgt agaaagttta gtggaaattc cttctgtcat gactcatgcg
     1021 tctattccaa aagaaaaacg agaagaggct ggcataaagg acggcttgat ccgtctttct
     1081 gtaggaattg agtatggtca ggatctcata aacgatttag ctcaggcatt tgacagaatc
     1141 aagaactaga agtcccaata aacgacttag cagaaaaatc taaaaaatat ttcagattgc
     1201 tacaccttgg aaacgataag tgttagaatc aagtagaaag acgtttttgt aaatttcact
     1261 aaataattct ttagtgaaaa gcatcaaaac gaatgaacaa acagatagga gtcttacaaa
     1321 tgagaatgaa tgtcgaaagt tttaatcttg accatacaaa agtcaaagca ccttatgtac
     1381 gtgtagctga tcgaaaaacg ggtgcgcatg gcgatgtcat cattaaatac gacgtccgct
     1441 ttaaacaacc aaataaagaa cacatggaca tgccaagtct acattcactt gagcacttga
     1501 ctgccgaact aatccgcaat cacgcagatt acatcgtaga ctggtctcca atgggatgcc
     1561 aaacaggctt ttacttgact gtattgaatc atgaaagcta cgaagacatt ttagatgttc
     1621 ttgaagcaac aatgaaagat gttcttgaag ctactgaagt acctgcaagc aatgaaacac
     1681 aatgtggctg ggctgccagc catacactag aaggtgcaca agctttggct cgtgaatttt
     1741 tagcaaaacg cgatgaatgg tctgaagtag aagcatagtt tctaaaagaa cgtaaagatg
     1801 agaagcaata tagctatctt atcttcgtat tttattaaat atttattgtc tgaaaagagt
     1861 tctaaacggg aaaacaaata gctagttttc agtttagcgc tctttttatt atataaaata
     1921 gaaaggagaa aactttatgt ctatttataa ttttgaaact actttagaag acggcactgt
     1981 ctattcattg aaaaaatatg caggcaaacc gataatcatt gttaacactg caacaaaatg
     2041 cggtctagca cctcaattta aacaattaga agaactttac caagcttatc agacacaagg
     2101 tctggttatc ttaggctttc cttccaatca atttaaacaa gagctagatt cagcgactga
     2161 agcagcagaa gcttgtaaaa ccacttatgg cgttaccttc cctatgcatc aattgattga
     2221 cgtaaatgga gaaaatacag atcctctttt tacttatctt aaaaatgaag ctcctggtac
     2281 tgtcactaaa gcaataaaat ggaattttac taaatttttg attgatagaa atggcaaagt
     2341 aatcgaacga tttgcaccac aaacttctcc attgaaaatg accgctgcaa ttgaatccgt
     2401 tctttaactt aataaaaata aaaaaagatg aactatttct agttcatctt tttgcctt
//
LOCUS       DACDIG010000153         2451 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.158, whole genome shotgun sequence.
ACCESSION   DACDIG010000153 DACDIG010000000
VERSION     DACDIG010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.158"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..105
                     /locus_tag="IV247_002850"
     CDS             <1..105
                     /locus_tag="IV247_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8778845.1"
                     /translation="VVTHSKEVADSADIILELSGKKLKKVNKMNLEVE"
     gene            105..1481
                     /locus_tag="IV247_002851"
     CDS             105..1481
                     /locus_tag="IV247_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8778846.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(1767..>2451)
                     /locus_tag="IV247_002852"
     CDS             complement(1767..>2451)
                     /locus_tag="IV247_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HAP8778847.1"
                     /translation="LYDEYFDCYICPQDQMLAFSTVTREGYREYKSNPKECVNCPLLN
                     QCTISKNHQRVITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKHGMR
                     WTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQARVTS
                     LEQLLSTVCDVGDISFSIRNQLRFKRSLPVSGNINVYFTKARFKRLIAITVSSIVMFL
                     WLFRMGVIP"
BASE COUNT          885 a          354 c          444 g          768 t
ORIGIN      
        1 gttgtaacac atagtaagga agtagcagat tctgcggata tcattttaga actaagtggt
       61 aaaaagttga aaaaagtaaa taaaatgaat ttggaggttg aataatgata aaaaatgcat
      121 ttgcttatgt aactagaaaa agtttaaaat cattaattat catattagtt attttatcta
      181 tgtcaacttt aagtctcatt agtttatcta ttaaagatgc tacggacaga gcttcaaaag
      241 aaacatttgc taatataaca aatagttttt ctatggagat aaatagacaa gtaaatccag
      301 gaacacctag aggtggggga aatgtaaaag gtgaagatat taaaaagata tctcaaacag
      361 atagtataga ctcctatgta aaaagaataa acagtgttgc agatttagtt gattatgata
      421 ttattgaaac tcaagagact cttgcgaatc aatcacctga aagagcaaag aattttaaaa
      481 gaacagttat gttaactggg gttaacgact caacaaaaga aacaaaattt gtatcagaag
      541 catataaatt agtagaagga aagcatttag aaaatgaaga taaaaataaa atcttaatgc
      601 ataaagattt agctgaaaaa aataacctta aagttggaga taagataaaa ataaaatcta
      661 atttatttga tgctgataat gagaaaggtg cagatgaaac agtagaagta gaaattaaag
      721 gtctattcga tgggcataat agcggtgtag taagtgcagc acaagaacta tacgaaaata
      781 cattaatcac tgatattcat acagctgcta aagtttatgg taataccgaa gatacagctg
      841 tataccaaga tgcaacattc tttgtaaaag gtgataagaa tcttgatagt gtaataaaag
      901 atcttgggaa attagatata aattggagag aatataattt gattaaaagt tcatcaaatt
      961 accctgcatt acaacaatct atatcaggta tttactcaat ttcaaataaa ttattttttg
     1021 gctcattaat atttgcagga gttgtagttt cattattatt attcttatgg atgaatgcta
     1081 gaaagaaaga aatagcagta ttactatcat taggtatatc aaaattagaa atttttggtc
     1141 aattcctaat tgagatggta tttatatcaa tccccgcatt cgttggttcc tatttcttag
     1201 ctcaatatac agctgacaag cttgggaata atatattgaa taaggttact ggtgatatag
     1261 ctaaacaaat agctaaacaa tctgcatcta gccaattagg tggtggggca gaagctgaag
     1321 gatttaacaa gacattatca agtttagata tcaatgtatt acctaaattc atgatttatg
     1381 tagttatatt tatgagttta gtacttcttg tatcattgat tatttcttca ttcaatatat
     1441 taagaaggaa tccaaaagaa ttattaattg acaataatta aaattttcgg tgctttttag
     1501 caccaaaaat gagagtgtaa tataaaccgt gtaagtaacc atcaatccta ggcgggtggt
     1561 tgcttcacgg tttttatttt tctaaaataa gatcaagagg tgattacacg atggctagaa
     1621 agaaaagaaa tcctgatgct gagaagttag ctgaatccat tttgaatact tatcaacccg
     1681 aatctgtaga ggatatgcaa gatgttttaa aagatgtgtt tggtccctta tttgaaaaaa
     1741 tgcttcaagg agaattgaat aaccatttag ggtatgacgc ccattctaaa gagccaaaga
     1801 aacatgacaa tcgacgaaac ggttatggca ataagacgct taaaacgagc tttggtaaag
     1861 taaacattga tgttcccaga gaccgggagg cttcttttga acctgagttg attcctaata
     1921 gaaaacgaga tgtctccgac atcgcagact gtagacaaaa gctgttccaa actcgtaact
     1981 cgagcttgga acagcttttt atctacttcg tttcgaattt ttgaacaaaa aaataaaggc
     2041 tccttgcctt tccatagcca ggtggctaat ttctttaaat tcattgcagc aaacactagc
     2101 atcgtatgcg tcgcgacttt ttccaagcct cgatacttgg tccaacgcat cccatgcttt
     2161 tcttttgcat ccgcaaatat tcgttcaatc gtttgtttcc gttttttgta aattgattta
     2221 ttcagatctg ttaggcgaag atgttccact tcatccatca gatctcccca aacatgtctc
     2281 gtgatgactc gttgatgatt tttacttatg gtacattggt ttagcagagg acagttcaca
     2341 cattcttttg gattggattt gtattctcgg tacccttctc gagtaaccgt tgaaaaagcg
     2401 agcatttgat cttgcggaca aatataacaa tcaaaatact cgtcatagag a
//
LOCUS       DACDIG010000154         2430 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.159, whole genome shotgun sequence.
ACCESSION   DACDIG010000154 DACDIG010000000
VERSION     DACDIG010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.159"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(1016..1306)
                     /locus_tag="IV247_002853"
     CDS             complement(1016..1306)
                     /locus_tag="IV247_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778848.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1514..2205)
                     /locus_tag="IV247_002854"
                     /pseudo
     CDS             complement(1514..2205)
                     /locus_tag="IV247_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
BASE COUNT          715 a          450 c          344 g          921 t
ORIGIN      
        1 acatacttgc aaagtaatac tacaattcct aacataatcg tagaatttaa taatttccat
       61 agcataaagt gtttagaaaa gaacattaag aatagttcta taataattct tgaactccat
      121 gtcttataat gttctatcga agcacccaaa tagccaagtt catctaatct acttgcataa
      181 actacgtcat ccgctctcat agtcggcata tacatattaa agagtaaaaa gataccccaa
      241 atccctatta gtaacaagat cagttctttg ttattcgtta tttttctatt atgtttgttc
      301 atttttatac tccttcttat atttcctaat agagtataac atagctttaa gcattactta
      361 aagataactt aaaagttatc ttttaaatac ttaattgtta aataccttat ttgatgcaaa
      421 agaaatctac gatagtcaac aaaaaaattt tcattatatc cagaaaaatt tttcttagtt
      481 aaggacctat ggattgcagt aatgcagaat tcgtcgaata aactgccaaa gacatacaat
      541 catattgctt ttaaaattga cgaacaagat atagattcgt ttgtctcaaa aatccaaatg
      601 ttaggtttaa ctgtcgaacc aggacgttct cgtgtgaaag gcgaggcttc ttcaatttac
      661 ttttacgact atgataatca cctcttcgag ttacataccg gtactctcca ggaacgttta
      721 aaatcctaca attccactac ctgagactag taatgactag tctcttttta tggcctctga
      781 tccccccatt gagcgaatac taatagggct tcgaattgat ctgttcaatc tttaagcaat
      841 atccctcaaa tgagtttgga aagtttagtg attcatcaca aatacttaaa tacatgtagt
      901 tatatataga ttgaggttct tgtgagcgta tgctcgtgtg ggttcaaatc ccatcgtctg
      961 catccatctt tccaaaacaa gcggctagga catttttgtc tcagccgctt gttttctagt
     1021 ttattctttc atcttgtcgg accttttttc ttgggtatag acttttctca tattctacca
     1081 caattttggg tttccctaac acatgatagg atctcacaac aaattgcttt ctattattat
     1141 aataacctgt aacgactaac ttcatccctt cgtctacttc agctaggaag ttcaaactat
     1201 gggaagcaat caaacaattg accccattta aactgaaacg gatcaacggt ctggcactaa
     1261 cttgcaaagt acggatctta tctactgttc catatttttt ctccataacc ttcacctcaa
     1321 taccattata cgaacataca ttccctttgt aaagaagaaa ctctgtttta tctttttgac
     1381 tttttgtcta ttacctttct taaaatcata ttggcacttc ctgcgaagat gaaggacatg
     1441 tactaaaaaa aagaggcagc agtatagatt actgttgctt ctttactaat ttaggtttac
     1501 ttgttaagaa agattattta cgcaaatgtt cttcaacaat ttgctgagat ttatcagctg
     1561 tatcttccac ttcatctttt gctttttcta acttttgaat atattgtttc aagcttgagt
     1621 tttcagcagt tgcttcgtct aatttctttt gtgtatctgt taactgattt ctacatttgt
     1681 tttttcttgt gtcagtgttg cgatttgttg attcaaacca tttacagtat tttgtaattc
     1741 tgaaagtttt gcttgtgctt cttgtagtct tgctgtccaa tctgcattca gatgattgat
     1801 ttcatcattt ttttggttga tctcatctag tttgacattg atttcatttt gtttgctttg
     1861 tagttgtgca tccttgctga ctaattgact ttctaagttt tgtttttctg ctgccgattg
     1921 atttttttgg ttttctaatt catttacttt tgtttggtat tcttggacat ctgcctttga
     1981 atcttgcaat gtctgttctt tttgtgctag tcgagcggta agatctgtaa tcgcttgctc
     2041 tttatctgtc aatgtatgat caagctgtgt cagattctca ttgattgtat caatatctgt
     2101 ctctccaccc cacaaaataa tttcatttgc gtaccgcgtg cctgtgtagc caattagaca
     2161 tagaaatgca acgacaatcc caccagtaat aaatttcttt ttcatttgca tagctcctta
     2221 atccaatact tttacatgct aaaagtaagt gatctcaaac acaatgtcaa gcgtttttaa
     2281 attacatatg taattttaat ataaatacta tcaaatactc atttaatcct ccttttttat
     2341 ctatttaaaa aatctttttt atagagaaaa atgcaggtta atactttctt aaataaatat
     2401 ttatggtgaa caaaaacaaa aaagcaagtt
//
LOCUS       DACDIG010000155         2399 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.160, whole genome shotgun sequence.
ACCESSION   DACDIG010000155 DACDIG010000000
VERSION     DACDIG010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.160"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            206..2068
                     /locus_tag="IV247_002855"
     CDS             206..2068
                     /locus_tag="IV247_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8778849.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2068..>2399
                     /locus_tag="IV247_002856"
     CDS             2068..>2399
                     /locus_tag="IV247_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8778850.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          715 t
ORIGIN      
        1 aaaatttgaa aaagcgtttg attatttata ttacgactta gtgcaataaa aaacgctttt
       61 taacagtaca tgtagtcgtc tttataatag ataaactctg taatattagt taaaaagcta
      121 taagttgcac ttagttaagt gcaaacattt catattgtcg ttagtttaga aaacgcttat
      181 attagcaatg taaggagaga tgataatgca gcagctaaca aaaagagaaa ggcagctttt
      241 gatttacttg gtggaaatgt ctgaaccggt aacaatcaaa aagttggctg aaaaagaaaa
      301 tgttagtgtt cggacgataa aatacgatct agatgatata agagagtggc tgaatgagcg
      361 gaagcaagtc ctatatagca aacgtagtca gggaatttgg atgaatatta gtagctctga
      421 tcgaatcaaa ttaaaaagcg aattgatgga tgttaatcgt tttgaaatat ttgcagatca
      481 aagtctccga atcaaacgtt tactaattat gcttttagta acaaaagaag ctattactgg
      541 aatgaaaatg gccaacgcat tagatgtcag caaagatacg atcatgaatg atttagatgt
      601 gctggaaaat aattttctta aggaaggatt gaccttgaat cgtcaggcgc gaaagggttt
      661 ttggatcata ggagaagaac gtctagttcg attaacgatt gaagagattc ttcaaaaaga
      721 gttcacggat tatgacattt ataaattaat gtcattgtta ttgaatggcg gtgagactga
      781 atactttgaa atgtattctg ctactgctac acctatccaa gaggtattta atcaagtaat
      841 cattcgaatg cgacacttac tggaatttga aaatctagag aaactgaatt acgcggaatt
      901 actaaatatt ctgattcgag tgacaattgc taccgttcgc ttacgcaaag aggctacgat
      961 tggtcgttat caattggttg ctgaacaaga attagacaaa aacgatcttt cttatcgcgt
     1021 catgcagcag gtctttgatt attatcagtt gccactgttc gaggatgaat atcattatat
     1081 ctatagtgat acattcgaca cgattcacca gcaagacgtc atgaaactag ttgagaatgt
     1141 gatttataaa gtaagtaaag caatcaacca ttctttttat caagatccac aattgttgac
     1201 gaatctgtat gctcatttat ccatgcgatt atcgagaaag aaaaaatttg tcaatgaata
     1261 taatcctttt aaagaagata tcaagcagaa atacccggaa ttatttgatg cgatcaagca
     1321 agcagttcga caagaaataa gtgaacaaaa tattctgatc aatgattctt tcgtggcata
     1381 tattgcctta cactttttgg tttcctacga aagagaagaa gatctacgaa gcgttagagc
     1441 cgtctacatt tgttcaacag ggttgggtgt gactagtctg atcaagcaaa agatcaatga
     1501 agaattaaat aatatcacta tcgtggcatt tgcttcagta ttgaatgcgc aagaaatgat
     1561 tgctaccaaa gatcctgatt tggtgattag tatttttcca attaatggca tcgatcgtcc
     1621 tttcatcaaa gtccatccat tacccactga acaggacttg aaaaaaattc aacaaataat
     1681 aaaaaaaata cttgcttctt ccaatcagca ggaaaggcat aacttgaatc ttagagaaac
     1741 gtccatcaat aaagatactt tagaagactt tagcaaggaa ttgattttga aagcttatac
     1801 gatttatgaa cgtttacttg tcattttgga agcagaattg atccaagaat atcgagaagc
     1861 ttttttgctt catgtattct taatggttca tcgtatcaca tttgatcaac aatatatggc
     1921 agaaggtcgt caagtagacc agattatttt agacaaagga acactggtag gaaaaatcga
     1981 agcagttttt gcagaaaata atttaggaat caatcagtca gaaatcagtg cattattctc
     2041 atatataaaa gaaggagcgt caagttagtg aaaatgtcag acgaggcttt aaaaattatc
     2101 gaggaaagtc aaaatcggga aaagttaaaa caagtgattg atctaattga agagcgatta
     2161 gtagaacatg ctattgtacc aaccgagttg cagtggacca ttctaattaa tcacttgaat
     2221 gagatgctga aacgttctca aagaaaggaa acgattcccg tagtcgatcg ggatttgttc
     2281 aaagaagtat ccaaagaagc cataactatt tctgatgaaa tagttagaag tatcggtaat
     2341 ttaaccgatg atgaaatata tgttttgtcg attcattttg aaacagcaaa aaataatta
//
LOCUS       DACDIG010000156         2351 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.161, whole genome shotgun sequence.
ACCESSION   DACDIG010000156 DACDIG010000000
VERSION     DACDIG010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.161"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..544
                     /locus_tag="IV247_002857"
     CDS             <1..544
                     /locus_tag="IV247_002857"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8778851.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(1017..1589)
                     /locus_tag="IV247_002858"
     CDS             complement(1017..1589)
                     /locus_tag="IV247_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8778852.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1605..2210)
                     /locus_tag="IV247_002859"
     CDS             complement(1605..2210)
                     /locus_tag="IV247_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HAP8778853.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          741 a          468 c          359 g          783 t
ORIGIN      
        1 catttcaaaa gagaatctct taaatcagga cttttctact gaaacgatct gtgagaaatg
       61 ggcagctgac attacgtaca ttcctactaa gaaaaatggt tggtgttact tatcttcaat
      121 catggattta cacacgaaaa gaattattag ttatacattt tcaaaacgaa tgactgtgga
      181 ttgtgtcatt caaacattga ataaagcaaa aatacattat cacattccag aaggaatgat
      241 tctacacact gacttgggca gccaatacac agcaagagaa gtggaacaat ggcttaaaac
      301 caacaaaata aggcattctt atagtagaaa aggaacacct tatgataatg ctggaatcga
      361 atctttccat gcctcattga aaaaagaaga agtctacacg actagctact cagattttga
      421 agaagcaaat cgagcgctat ttagctacat tgaaggattt tataaccgaa atcgaatcca
      481 tagctcgatt cactatctaa ccccacagga atttgaagaa ttagcaaaag aaaaaatggc
      541 ttaaccgtct ctactaaaat gtgtccaaga tattgactca aatccagtaa tatcacaata
      601 ggaatgctgt ttattatatt agcagcagtg ctgattttta tgatagttat ttggttctta
      661 aatgaaagat ctaaagacaa tcaacaccgt agggatatgg agataaaatc aatagagtca
      721 atagattatt tatcgataat aacactaaaa aagccacgtt cctaaacgtg acttacgctg
      781 aacgatatgt gttgtttggt agacgaacaa atcgttcatt ataaatatat gatggtatcc
      841 taatatagta agcgtggcta gcataccttt aattacttta ccttttacag ttaccataca
      901 cattttttga gcagaaatca agtaaataaa aaaatattat atttcacaac atgctaatag
      961 actcatatct agaacgcatt aagtatttac ctgtctggtt cgctgagact tcttttttaa
     1021 ctttcttcaa ttttcttctt tattctaaaa agcgtactgc gactgaaacc ggttatagct
     1081 tcaacttcct tatacgattt acctgaagta agcagttcat atgcgtgcct cttcttcggt
     1141 gtgattgttg ctttcggacg tccttctctg aaattaggat tattctttct tgcaaacatt
     1201 tttccttctt gggtacgttc cacaatcata tcacgttcca tttctgctac actcaacaac
     1261 gtccgtgtca ccattcgccc catggacgta ttttcaatcg ttcctagatt caaaaccttg
     1321 atcgttacgt tatcatcgag taaaggttca atgatctcta atgcttctct cgtgttacga
     1381 gcaaatcgat caagcttcgt aacaactatt tcatctccag gcttcagacc agccaacagc
     1441 tgattgaatt ttggccgatc ccgttttgta cccgtaaatt tttctgaaaa aatattcttc
     1501 ttctctacgc agttctcaag caactgttga atttgttctt ttgtactttg agaagcggtt
     1561 gagacacgtg catatccata tttcgtcatt tggtccacct acttttacaa atcatctatc
     1621 gtatactcag aatacccttc atagaaatag ctgacatcga ttcctttcat atataactcg
     1681 cgatcgtcaa ttttatcagt cagagcattg gaaattagat agcgtatttc caaatcgttt
     1741 atgggacttc gctccattgc agaaagatag tctgctttat cgattaagtt ccaatctaca
     1801 actttttgca attcttcctt taaaatgaga tccagccaaa ttctagtact tcggccattt
     1861 ccttctcgaa atgggtgagc gatattcatc tccacatatt tcttgataat ctcttcaaaa
     1921 gaagtttgtg gcatctgatc aatatgctgt aacgaatgtt ctaaatacat aactggtgca
     1981 aatcgaaaat ttccttttgc aatattgacc gatcggattt ttccggcaaa atcgtaaata
     2041 tccgaaaata gaaattcatg aatttcagat aaacctttaa atgtaccaac ttcgatttca
     2101 tttatttttc cacaatcgta caattctttc gctttttgtt tactgagttt ttcttcaacc
     2161 ttagccaact caacttgatt ggttaatccc aatttattct ctaaaatcat gcgaaatctc
     2221 cttaacttta tgacataagt ttatgaaaca ctctcaaccc cattatatcg cgattttgtt
     2281 tatgtgtcaa aaactattaa gtttctgacc cccgatagat ttttcaagtg tctctatttt
     2341 gcacagtcta g
//
LOCUS       DACDIG010000157         2331 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.162, whole genome shotgun sequence.
ACCESSION   DACDIG010000157 DACDIG010000000
VERSION     DACDIG010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.162"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            31..2184
                     /locus_tag="IV247_002860"
     CDS             31..2184
                     /locus_tag="IV247_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAP8778854.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          801 a          415 c          521 g          594 t
ORIGIN      
        1 catgaatgta aataggcgga aggataggaa atgatgaaag attttatgaa aaaaatatta
       61 aatacttctt ggttagaaaa aataagaaaa ggtgcacaag aacctaagaa caaaacgttt
      121 cgccggtccc atgttccttt tcgcttaaat tttctttttt tcattatatt tacgttattt
      181 gttgcgttga ttgctagatt gggttatttg cagatcgtga acggcgaaga gatggaggcg
      241 cgagtgaagt ctacttctac gattacggtc caagaaagta ctcctcgcgg aatgatctac
      301 gactccacag gaaaagcttt agttacgaat aaagcgaatc aggccatcac atatacgaga
      361 ggaacacaag ttacggctaa agatttgctt gaaatcgcaa cgaagctgaa ctcgctgatc
      421 gatgttccgg tagatgaaaa tctgacagat agagataaaa aagatttttg gctggctgat
      481 cccaaacatt tgaaagaagc cacaaaaaga ctttcggcaa aagaaaagca attagggaac
      541 agcgaacaat atagtgcgat ggtcaataaa gtcacagctg atgagattaa tttaaacgag
      601 gagcaattaa aaattgctac catctttaaa cggatgaata gtgcatatga attaaacact
      661 gtctatatca aaaactctgg tgttactgat caagagttag ctgttgtggc agaacgcgct
      721 tctgaacttc caggagtttc gactggcaca gactggacac gagaatataa tgcagcaagt
      781 tcactgaaaa gtattcttgg cagtgtgacg actgaaaagc aaggacttcc agcagatgaa
      841 gcagagaaat acctagcaaa aggttattct cggaatgacc gtgtaggaca aagttatttg
      901 gaaaaacaat atgaagatgt cctgcaagga accaaaaccc aatatgaagt ttcccttgat
      961 aatgaaggaa atgtttcgaa tcaaaaagaa atcttctctg gggaaaaagg ttctaattta
     1021 atgttatcta tgaatgcaga attccaatca aaagtagaag aaattttgaa aaggaattat
     1081 caaacattga tcaacaacgg caaagcacag tattcgcctg gtgcttatgc agtggctatg
     1141 aatcctcaga caggagaagt gctagcaatg actggtttca gtcatgaaca aggttccaaa
     1201 gaaatcaccg agaatgcttt aggcacgatt actagcgcct ttgatccagg gtctgtagtc
     1261 aaggctggta ctttgacagc aggctgggca agtaacgcga tcagcggaaa tcaagttttg
     1321 attgatgagc cgattcgtct gcaaggtgct tcagaaaaaa gctctgtctt taatcgaagc
     1381 ggacaagttg ctcttgatgc agtcaaagca tcggaattgt cttctaatac gtatatgatc
     1441 aaagtggcgt taaagatgtt aggactagac tatacacctg gaatgggact acctagttta
     1501 gatgaagaag ctaaagctta tcagcaattg agagactctt tcaaagagtt tggtcttggt
     1561 acaacgactg ggatcgattt gccgaatgaa tctcctggga tctcacgttc tgttgattat
     1621 atgaagaagt tcaatgcgga taatggcaaa gaatggtatg ctccaggtaa ctttaccgat
     1681 ttggcatttg gacagtttga cacatataca ccaatccaat tagctcaata tgcttcaaca
     1741 gtcgctaatg gcgggaaacg agtacagccg cacctagtca aagcgatcta tggcaatgac
     1801 gaaaatggaa acttgggtga agtgaagaaa gaaattggca caacagtaga aaatacggtc
     1861 aatatttctg cagaaaatat ggcgatcctc cgtgaaggat tccaccaagt tgtccatggt
     1921 accgatcctt acacaacagc caaaccatta gccagtgcga aaatggacct ttccgcaaaa
     1981 acagggactg cagaaacggt tgcggaaggt catccagata ttgcgacagt caatagtaat
     2041 atcgtggcat acggaccaac agacaatccg gaaattgcca tcagtgttgt cttgccgaac
     2101 ttattagacg aacaagacca cataaacttg acgattgcta aagaaataat ggacgcgtat
     2161 tatgatatgt tcatggcaaa ataaaaaaga ggtcgtgaaa aagccgctta gtttcgagta
     2221 tgaagaaaaa ttttgtgaaa atagcttctc acatttttgc ttaaatttga cttcataccg
     2281 agaaactggc ttttgaacac cgtttactcg gcaaaaaagg ttgtgacaaa a
//
LOCUS       DACDIG010000158         2304 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.163, whole genome shotgun sequence.
ACCESSION   DACDIG010000158 DACDIG010000000
VERSION     DACDIG010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.163"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1266
                     /locus_tag="IV247_002861"
     CDS             <1..1266
                     /locus_tag="IV247_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP8778855.1"
                     /translation="LNTKAKDPTDAIFGIKPDGTIAHFCISDTPHMLVAGTTGSGKSV
                     LLNEILVTAICHAMPDELKLGIVDPKRVEFGRYKKLPYMLADPITDMDEAYEFFEYLV
                     ILMNERYKLMEKAGVQNITQYNKYAEKHGIEKFPYVILLVDEYSQLVGTHKEVEGLIV
                     QLGQMARAAGIHVILATQSPRSTVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGES
                     LSPKGDMFIKLNGGNKIRAQGAYISNEEIEAIFDHLRNTMPEPEYVDYRAIVAESRGE
                     GGEDEDFGGIGKAPVRKSAKRNLNTPLANSSSKEPSIEKKSTFALKLEKNKEEKEKQG
                     AKTLEKLQAEKLEKVLKALDKVDPEPQTSNNTNNEQPISSKTKPKTTDPKVSVKVTIT
                     TDNSEKKQVNGTTSPIMQKMLKAKKQVHK"
     gene            1263..1892
                     /locus_tag="IV247_002862"
     CDS             1263..1892
                     /locus_tag="IV247_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778856.1"
                     /translation="MIIVSSFLRWLTPYKYTDEVVVKRAKKKDKKAVLTPKQMLDHGI
                     LGVKVLEVNKPFESNSAREKLLGAEADVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLRPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSNQNRVQINKFEKKVRK
                     FNERKTILWFADICKQPDGTAKWEAVIFDENGKKVMSETWHDKAKFVWQ"
     gene            1904..>2304
                     /locus_tag="IV247_002863"
     CDS             1904..>2304
                     /locus_tag="IV247_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778857.1"
                     /translation="MLTILKLYREGFNEKVSIGESIEYRGDKYIIIRILKINKHYFSK
                     SISLTVRVLVQKVGDLSDYYNCKREVIVDNRYDQSKDDKRIIKIGDIVFPEKGIAYEV
                     VSINSFRYEFVDLVVEYSVRLIVPWSKEEIN"
BASE COUNT          871 a          344 c          483 g          606 t
ORIGIN      
        1 ttgaacacaa aggctaaaga cccgacagat gctatttttg gaattaagcc tgatggcaca
       61 attgctcatt tttgcatcag cgatacgcca cacatgttag ttgccggaac aacaggatca
      121 gggaaatcgg tattactgaa tgaaattctt gttacagcta tctgtcatgc aatgcctgat
      181 gaattaaagc tagggatagt agacccaaaa cgagtagagt ttgggcgtta taaaaaatta
      241 ccctatatgc ttgctgatcc aattactgat atggatgaag cttatgaatt ttttgaatac
      301 cttgtgatct taatgaatga acgctacaaa ttgatggaaa aagcaggcgt tcaaaatatc
      361 acacaatata acaaatacgc tgaaaaacat ggaatagaga aatttcccta tgtgatctta
      421 ttggttgatg aatattcgca attagttgga acacacaaag aagttgaagg gctgatcgtt
      481 caattaggac aaatggcaag ggcggcagga attcacgtta ttcttgcaac acaatcacct
      541 cgatctacag tcgtgacagg tattattaaa gcaaatttcc catctagagt ctgcttgatg
      601 gtagcaagtg atttagaaag taggatcgta ttagatgagg gtggtggaga aagcctaagt
      661 ccaaagggag atatgtttat caagttgaat ggaggaaaca aaatcagagc gcaaggagcc
      721 tatatcagca acgaggaaat tgaagcgatc tttgatcacc ttagaaatac tatgccagaa
      781 ccagaatatg ttgattaccg agcaatcgta gctgaatctc gtggtgaggg cggtgaagac
      841 gaggattttg gtggaattgg taaagctcct gtaagaaaat ctgctaaaag aaatttgaac
      901 acacctttag caaattcatc atctaaagag ccatcaattg aaaagaagag tacgtttgct
      961 ctaaaacttg aaaaaaacaa agaagaaaaa gaaaaacaag gagctaaaac actagaaaaa
     1021 cttcaagccg aaaaattaga aaaagtttta aaagcattag ataaagttga tcctgaaccg
     1081 caaacctcaa acaacacgaa taatgaacag ccgatttcat ctaaaactaa accgaaaact
     1141 actgatccga aagtttcagt aaaagtgacc atcacaaccg ataattctga gaagaaacaa
     1201 gtaaacggaa ctacttcacc tatcatgcaa aaaatgttaa aagccaaaaa acaggtgcat
     1261 aaatgatcat tgtcagttct tttttgaggt ggcttacgcc atataaatat acagatgaag
     1321 tagttgtgaa acgagctaag aaaaaagata aaaaagcggt gttaactcct aaacagatgc
     1381 ttgatcatgg aattttgggt gtgaaggttt tagaagtgaa taaaccgttt gaatcaaata
     1441 gtgcaagaga aaagttgctt ggcgctgaag cagatgtggc atatttttat catgacaaaa
     1501 gcaagcatca aatagctatt aaattcaatc caaaatcggc atggcggtat caagcaagag
     1561 atgacttgaa acgtaggaaa cttagaccaa tgctctatcc agaaggaaaa gaagcatttg
     1621 atagaaccca catcgttccc atcggttttc atggtacaga atcagataat cgcctattag
     1681 taggttggaa cagcaatcaa aatagagttc agatcaataa atttgagaag aaagtaagaa
     1741 aattcaacga acgaaaaaca atcctatggt ttgcagatat ttgtaaacag ccggacggaa
     1801 cagcaaagtg ggaagcggtg atatttgacg aaaatggtaa aaaagtaatg tctgaaacat
     1861 ggcatgataa agctaaattt gtttggcagt agaggtgaaa attgtgttaa ctattctaaa
     1921 actatataga gagggattta acgaaaaagt aagtattggt gaatccattg aatatagagg
     1981 tgataagtat attattattc gcattcttaa aattaacaaa cattatttta gcaaatctat
     2041 tagtttaaca gttagagttc tagttcagaa agtcggagac ttgagtgatt attataactg
     2101 caaaagagaa gttattgtgg ataatcgata cgaccaatca aaagatgata agcgaataat
     2161 aaaaatagga gatattgtct ttcctgagaa agggatagca tatgaagtag tgtctatcaa
     2221 tagttttcgc tatgaatttg ttgatttggt tgtcgaatat tctgttcgat tgatcgttcc
     2281 gtggagtaaa gaagaaatta atga
//
LOCUS       DACDIG010000159         2225 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.165, whole genome shotgun sequence.
ACCESSION   DACDIG010000159 DACDIG010000000
VERSION     DACDIG010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.165"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..273
                     /locus_tag="IV247_002864"
     CDS             <1..273
                     /locus_tag="IV247_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778858.1"
                     /translation="VLYFLAFVFSLKPGFTAMKFFGDAKWGDGLKYVGAIIAIFIIPV
                     ITTVMLYTMGTKTGNDLNQKANIISALIPVVGIYLVSKFPKNELLG"
     gene            383..604
                     /locus_tag="IV247_002865"
     CDS             383..604
                     /locus_tag="IV247_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778859.1"
                     /translation="MVFDREKMLAHANEVLMSSLKGTELAKIMNMNVNQFYDYRNGSK
                     KIEKARLETLIKFEKAYVYMLDKQKRTID"
     gene            624..>2225
                     /locus_tag="IV247_002866"
     CDS             624..>2225
                     /locus_tag="IV247_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778860.1"
                     /translation="MAKFKDITESINYVAETVPVVALSLKPQLLEVYKKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYMVIEDVQKLNSEGVIDEDDAILDSEKYVVLLKSSLN
                     YVADLPEKFKEKLLAGVRFMDSSGQLLVIPDDPNVFESEVEEATDFYSAVEQLEVATN
                     GLERIAETFNSSEEVEFEAVAENVPDEKEALFVDDNKPTEAENQEVSIEETQIQTETY
                     QQPKIDEKPESQSDKENYVYEAEKITSESDVPKNLDADFLEKNFEQIIADKFQPVVLD
                     EINDPVDLNEEMLSIPELKPLLFLAKESMNARISIANNKINSELNKTKEQLILKGKQM
                     LADELLRIEEATSLNGQNQFTNTLSVAKESYEAEASKIDDLVMEKSNIIRKDYDMAKQ
                     AYLDAELKKLEQRYDAEHLPEVKQQEDEYRKATEESLDQQYQEAIESIKNDALKQSQE
                     AKEVVVERIINSLKPEIEKSLTDVQEKTLEIVEKTKSENEAEFHDTKEKTTDVVVKYF
                     ANKEEVEKQVANNTKELETKLVEQQK"
BASE COUNT          889 a          312 c          441 g          583 t
ORIGIN      
        1 gttttgtatt tcttggcatt cgttttttcg ttgaaaccag gattcactgc aatgaaattt
       61 tttggtgatg caaaatgggg agatggatta aaatatgttg gggcgattat tgcaatcttt
      121 attattcctg taattacaac cgttatgctt tacacaatgg gtactaaaac aggtaatgat
      181 ttgaaccaaa aagctaatat catatcagca cttattccag ttgttggtat ctatctagtt
      241 tcaaaatttc caaaaaatga actgttaggt taaaagtgaa ttgaacaaat ttggataata
      301 tgccgatttg ttcaatttac tttttaccaa aaaactacgc tccaagcgta gtttagaagt
      361 gagggaagaa agtaaatgta agatggtttt tgatagagaa aaaatgttag ctcacgccaa
      421 cgaagtatta atgagcagtc tgaaaggtac cgaactggca aagataatga atatgaatgt
      481 taaccagttc tatgattatc gaaatgggag taaaaagatt gaaaaagcca gacttgaaac
      541 gcttattaaa ttcgaaaaag catatgtata tatgctagat aaacagaaaa gaactattga
      601 ctgaaaaaag gagttttaca ataatggcaa aattcaaaga tattaccgaa tctattaatt
      661 atgtggcaga aacagttcct gtagttgcac tctctttgaa gccacaatta ctcgaagtgt
      721 acaaaaaaga agaaaaagaa gcgaaggcaa aaaaagctaa agcaaaggta tggaaagaat
      781 tcgatgcgtt gctgaccaaa gatttaccaa agaaaaatta tatggtaatt gaagatgtac
      841 aaaagctcaa cagtgaggga gttatagatg aggatgatgc tattctcgat tctgagaagt
      901 atgtcgttct attgaagtca agtttgaatt atgtagctga tctaccagaa aaatttaaag
      961 agaagctatt agcaggtgtc cgttttatgg atagtagtgg tcaattatta gttattccgg
     1021 atgatcctaa tgtgtttgaa tcagaagttg aagaagctac agacttctat agcgcagtag
     1081 agcaattaga agtagctact aatggattag aacgaattgc tgagaccttc aactcttctg
     1141 aagaagttga atttgaagcc gtagcagaaa atgtaccaga tgaaaaagaa gctctttttg
     1201 tggatgataa caaaccaacc gaagcagaaa accaagaagt aagcatagaa gaaacacaaa
     1261 tacaaacaga aacctaccaa caaccaaaaa ttgatgaaaa acccgaatcc caaagtgaca
     1321 aagaaaatta tgtttatgaa gctgaaaaaa tcacatctga aagtgatgta ccgaaaaact
     1381 tagatgctga ttttttagaa aaaaattttg aacagatcat tgctgacaaa tttcaacctg
     1441 tagttttaga tgagattaat gaccctgtcg atttaaacga ggagatgcta tccatacctg
     1501 agttgaaacc attgctgttt ttagctaaag agagtatgaa cgctcgcatc tctattgcta
     1561 ataataaaat caacagtgaa ttaaataaga cgaaggagca attgatcctc aaaggaaagc
     1621 aaatgttagc tgatgagctt ttgcggatag aggaagcaac ctcattaaat ggtcaaaatc
     1681 agtttacaaa tacactatcg gttgctaaag agagctacga agcagaagca tctaaaattg
     1741 atgatttagt gatggaaaaa tccaatatca ttcgtaaaga ttatgatatg gcgaaacagg
     1801 cttatttgga tgctgaacta aagaaactag agcaacggta tgatgctgag catttaccag
     1861 aagttaaaca acaagaagat gaatatagaa aagcaactga agaaagtctt gatcaacagt
     1921 accaagaagc aattgaatct attaaaaacg atgcattgaa acaaagccaa gaagcaaaag
     1981 aagttgtggt ggaacgaatt atcaattcat tgaaaccaga aattgaaaaa tcattaacag
     2041 acgtgcaaga aaaaacttta gagattgtcg aaaaaacaaa gtcagaaaat gaagctgagt
     2101 tccatgatac aaaagagaaa acaacagatg tggttgtcaa atattttgcg aacaaagaag
     2161 aagttgaaaa gcaggtagct aacaacacga aagaattaga aacaaaactt gtagagcaac
     2221 aaaaa
//
LOCUS       DACDIG010000160         2118 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.166, whole genome shotgun sequence.
ACCESSION   DACDIG010000160 DACDIG010000000
VERSION     DACDIG010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.166"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            303..1334
                     /locus_tag="IV247_002867"
     CDS             303..1334
                     /locus_tag="IV247_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8778861.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            1443..1868
                     /locus_tag="IV247_002868"
                     /pseudo
     CDS             1443..1868
                     /locus_tag="IV247_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
     gene            1981..>2118
                     /locus_tag="IV247_002869"
     CDS             1981..>2118
                     /locus_tag="IV247_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP8778862.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LK"
BASE COUNT          759 a          364 c          350 g          645 t
ORIGIN      
        1 attctgaaaa aatgttttct aatttttcgg aaataaaaga aagagcctcc tgccattaga
       61 acgaaccaaa tccatttcat tgctcatgaa gtaaccacat agctaaaaag aggataaatc
      121 aattaaaaga tcaaaagaat gactgcacta ataaaataac taaaaatgta tcttcttact
      181 tcatatcttt tagctagtac ttagttaaaa atttgcttcc taagatcaag acaaactgtc
      241 tatcttcatt tgatgaaatg cggtaaaata tagaaaatga acactattgg agggaatttg
      301 taatggacag caaaactaga aaacaactgc aagaaaagtt aaagcaacgt acacacttgc
      361 tcccttccgt ctttcaacag tttttagaag aaaatcgttc ttccctttca ttaggcagtc
      421 gctatgaata cgcaaaagat tttcatctgt ttactgatta tttgagaaaa gtattttctg
      481 aagaagtgga tgatcagctg attatccatt tagaaaaaca agattatcag aatttcttag
      541 ctaaaatcta tcgacatact cgttcgttcc aaactacagc gaaccaagaa acggagcaat
      601 tgtttgaaaa tagctattac gggatttcaa ggaagcaata ttcattaaat cattttttac
      661 gatttttgta tcaaaaagga ctgattgaag aagagatttc tcttttgggt acaagtttac
      721 cggaaacaac caaagcagct ccgcgatata tcgatgcaga acttttcaaa gattttgagc
      781 atttgtttat cccgattgaa ggctttcaaa caagcagaga agctagcttt gaagaaaaga
      841 atagaccgag gaaccttgca attactgcca ttcttctgta ttgcggatta aagatccatg
      901 aagtagtaga acttaaacgt aaagatgtat ctttgaccaa gcagattctt aatctgaata
      961 gaaaagaaaa cagactgaat aaagtaccaa taccaaaaga agcgatgcca tttctggaga
     1021 actattttaa gttaacagca caaaatacgg acgaaaattc ttttgttttt cgttcctcac
     1081 atgatcagca aatttctcct cggactattc gccaaatttt gagcaaaatt acagtatata
     1141 aaaacgtatc gccagaacta tgcagaaaaa catatcgtta ttatgcagaa ctatatttag
     1201 atgatgctga cgcggtaaat tatttatgtg gtaatcgata cctctcatcc cattcttttc
     1261 aagatatctg ggaaaagatg gttgattttt cttatcacga attagcggca ttcgttcggg
     1321 tttcttccat ataaaacaaa tgtatgatct ttttttcaca ataaactatg ttattatata
     1381 aaggattatg ttacgattat cttgaatttg tatattactt aacaaaagat aatggagtgt
     1441 gaatgatgga gaccataacg tattcccctt tgaacttatt tacgaatttt caagaagctg
     1501 cagctaactt ttccgagacc ccgattatct ttgatgagcc actttcagct ttcccagaat
     1561 taggacttga aacaacttac aaaaaatgct gcgaaagtgt gttgaaacgg gcttatcaat
     1621 tggctcatct tggagtgaaa tccggagaca aagttattat ttataagagt tccgcttttg
     1681 acacatacct tctagctgtt tctgtgtctt accttggtgc agtgcctgtc atgacttctt
     1741 atcatctacc aacaacaaca atggaagtat ttatcgatcg tttagaagac cctttcattt
     1801 tatttgatga tgagacaaaa aagcgagtga gagaaatttc aaatggaaca aaatcaaaac
     1861 agattcctaa cccgtggtgc tagttaattt tttcctagag gaaaatgtct aaacatgttg
     1921 gtaaatcaac aaaaaaacgc atatagtagt ctcaaaactc ccaagaaatg aggactacat
     1981 atgcgttcat caaaatatac tgaacattat acagatactt ttataacatt caaggaaatt
     2041 atgagaactg tttctaacat ataccacaat tgtgtaccag ataagattaa aaaccgaaga
     2101 aatactgatc aactgaag
//
LOCUS       DACDIG010000161         2108 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.167, whole genome shotgun sequence.
ACCESSION   DACDIG010000161 DACDIG010000000
VERSION     DACDIG010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.167"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(557..922)
                     /locus_tag="IV247_002870"
     CDS             complement(557..922)
                     /locus_tag="IV247_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778863.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(998..1243)
                     /locus_tag="IV247_002871"
     CDS             complement(998..1243)
                     /locus_tag="IV247_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8778864.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1313..1567)
                     /locus_tag="IV247_002872"
     CDS             complement(1313..1567)
                     /locus_tag="IV247_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778865.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1731..2054)
                     /locus_tag="IV247_002873"
     CDS             complement(1731..2054)
                     /locus_tag="IV247_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778866.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
BASE COUNT          790 a          361 c          370 g          587 t
ORIGIN      
        1 tcgtctgaaa acaaaaaaag aaagcgttat tattttagtc tattttcagg gggggaacct
       61 tatgataaaa aaactatttt tactatcaat attaacgcta gcaatggcag gttgttctga
      121 ctctggctta gcaactgaac tgatcacaaa atcaactgaa gccacgacag aggagttaaa
      181 atgtggaaac gatcacagtg actacagagg cttaaatcga cgatgcatgg gaatattagg
      241 cagaagcaat tgtaagcatc gatgtagtaa tcgaagaagg tgtagaagaa gcaacagaaa
      301 aatcaaaaga aagatcaaaa gaagcaatct gtgctgtgca tcgtatacag acgtcgaaga
      361 tgcggtactt ttaaaccacg tcaggagatg ttcatttatg tctagggaag ttggcgatga
      421 accaaaacta gagtacgctt ttagttttgc acgtgaggat gaagataacc ctaaaactta
      481 cgacatttta gcttgatcta agatggttaa agctttttaa tgaagtaaga ggagatagaa
      541 ggcttttcta gttatattag gtaagataaa ttcctttttg ctccaaattt gctaaacatt
      601 ctgctagata aacttcatcg tgttccggat aaatcggcac gttgcgctct acagtaggta
      661 aatcaaaaaa tgcttcgcat gatttgccac gatacatagg atttttccac tctggtgctg
      721 ggctgtaacc acgtttttcc atttcatcca tgacaagctg atgatattgg aataatctat
      781 aaggagaata gtgaaaaaca taatccacag tagcatgttt ctttccccag ccatttcctc
      841 ttaatgcaca gcattcccga tgttgtccta aaagctgttg acgaggaagc tttgaaatca
      901 atttttcatg ccataatcgc atagaaatgt ctcctttata tatagattgt taaccttaga
      961 cgttaaatga aaaaaccagc accaaggctg gaaaaaatca cttcagtttg cttgctgttt
     1021 ctgagcgacc aattaggtaa tcaacggaaa tatcgtaaaa gtcagcgata tagattaatt
     1081 caatgatgct tggaatttct tctccttctt cgtacttacg atatttgaat aaaggaatac
     1141 ttaacgcgct agaaacagct acttgagata aagatcggcc agttcttaag aaattcagac
     1201 gacaacgtaa agtatccagt aaaatagctt cttcgatact catttaatca cctttcatta
     1261 tagtagaata gttataatga ctatagaaag ctacacatgc aatcaatagt actcaatgtt
     1321 ttttatttaa acgtatcaaa taaagaatga cgatcaataa agacaaaacc agtcctgata
     1381 attcttctat aaaaacacta tcgggttcaa agtagttgta attaaaaact ttccacataa
     1441 agtagatagc cggaagagtc aaaatagcta aataaaaccg ttctttgttc caattcataa
     1501 gataaacaat acttgcgacc agtgcgaccg caggcaccca aaacaaaact ctcaccaacg
     1561 taatcatgta acggactcct caacgattca tataataaaa gataacatgt tattgcatac
     1621 atcacaaatg aaatgctagg aaaataaaag aagaatagat caatgaaaaa tgaaaaagca
     1681 gaacagtttt ctgctgataa gcaggaactg ttccgctgaa tgtgtcttat ctatcgagaa
     1741 aaaatcaatg gattgatatc taagtaattc aatacatata aaaagaggac gatcgcattc
     1801 actaaaaagg aaagaatgat gatcccatag cgaatggaaa catatttaac aaatactttt
     1861 gtttgaatac gggcatatct ttttccttta tagctgagca tcatgacaag aagcaaaagg
     1921 gtaatcacac cttgaaaaat cattccaata aacagaattc ctgaggtttg ataatttaat
     1981 tggagaaaat aagctgctgc cgcaactaaa tcgaataaaa tcgcaataaa tggcaaaata
     2041 atcactccta acattgagaa taaataaaag aataacattt tttctaaaat aagcaaataa
     2101 aaattaaa
//
LOCUS       DACDIG010000162         2090 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.168, whole genome shotgun sequence.
ACCESSION   DACDIG010000162 DACDIG010000000
VERSION     DACDIG010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.168"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(116..373)
                     /locus_tag="IV247_002874"
     CDS             complement(116..373)
                     /locus_tag="IV247_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778867.1"
                     /translation="MINEDFRSENKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGLRSYTTSEKDK"
     gene            complement(376..600)
                     /locus_tag="IV247_002875"
     CDS             complement(376..600)
                     /locus_tag="IV247_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778868.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEYKENIPKNLQGSER
                     YEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            complement(773..1816)
                     /locus_tag="IV247_002876"
     CDS             complement(773..1816)
                     /locus_tag="IV247_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyhydrolase"
                     /protein_id="HAP8778869.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGTPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     VPNPTPAPSTEKVLNVPVVWQGNTMLCEGASLLEGLHYKGVATNQSLTSFVNYMPKAN
                     DNNPYHGYSGEWRHNVNGTYQGMMADPVVKWAKNVGGNAVNITGSGTNGIKNEIRNGN
                     PVVTWITYNYATPEFKQMPWGRAVWNGHVVLVDGFRNGAYHVVDPVFGAKWINASTYE
                     RAFNVTGMAVAVR"
BASE COUNT          564 a          454 c          296 g          776 t
ORIGIN      
        1 ttttcgctta tcatttaata tttcccttcc tttttttgca ttatatttag caaacattca
       61 ccatttgaat ttgacatagc tttaagacag atattttatc ttttcaaaca ttaagttatt
      121 tgtctttttc agaagtggta taggatcgaa gtccttcacg tttcattatg tcttctgctt
      181 ttccaatgct gatccctttc caatcacgcc atcctacgct tcccttattt tgacgatagg
      241 ctttaatagc tgattcggat atttgccatc ttgtagccag ctcttttgcg gtgtacttat
      301 ttgagaaaag taacttctct atcagtgaaa tatcaatcat tttattctca ctcctgaaat
      361 cttcattaat catctctagc tagctatatc ttcaatcaat tctaatatgt cggataaacc
      421 atcaacagct tcttcaagtt gatcaacgga ttcttctgct ttttcatatc tttctgatcc
      481 ctgtagattt ttaggtatat tttctttgta ctcttcttct tgaattactt ctaactcatc
      541 ctttagctcg gaaagtcgct cgcttatttc ttctaacctt tttcttcttc tttggttcat
      601 caaaatcacc tcttcgcata atctctacga ttttaatata ccacttttgt gtactacatt
      661 caatattttt tgttatagat tcgtattaca aaaatttcct ctaaaaaaat aagagcctat
      721 ttaccatttc agtaattaag ctcttatttc atttagttga taattctatc aattaacgta
      781 cagcaaccgc cattccagtc acattaaatg ctctctcata cgtgcttgca ttgatccatt
      841 ttgcgccaaa tacagggtca acaacatggt atgcaccatt tctgaagcca tccacgagaa
      901 ctacatgtcc attccatact gcacgacccc aaggcatttg tttgaactct ggcgtagcat
      961 agttataagt aatccaagtt acaaccggat taccattgcg aatttcattc ttgattccat
     1021 tcgttcctga accagtgata ttgacggcat taccgccaac gttttttgcc catttaacta
     1081 ctgggtcagc catcatgcct tgatacgttc cattaacgtt atgacgccac tctcctgagt
     1141 aaccatgata aggattattg tcattagctt ttggcatata gtttacaaaa ctggttaagc
     1201 tttgatttgt ggctacacct ttgtagtgta aaccttctaa taaagaagct ccttcgcata
     1261 acattgtatt tccttgccat acaacaggaa cgttcaacac tttttcagtg cttggagcag
     1321 gtgtaggatt tggtacactg tttggcattg cataccagcc tgtaccttca atcttccaac
     1381 cttgtttact aattacgttc ttttcatttt gatctaacgt gtagtgatgc caattcacgt
     1441 atttgttata taaacgtgtt acaggaactc tatttgctcc gttgtaggaa tagaacgcaa
     1501 caccttcgta cgtccatcct gtgtgtgaaa tcttatctct ttctccggca tcaactgtgt
     1561 aaaaatgttc accgttatac cgattataca aacgataaac aggagtacca cttgttggag
     1621 cttgccatgc gacaccttcg taatgccatc cataacgttt cagattgtct ctttctccaa
     1681 cgcttctagt gtatagatgc tctccgctat tgggattata catacgatac atggaaatag
     1741 agctttctgc tgaaactgaa ggtgataata atactagccc cattgtgaat agaattccta
     1801 ctaaaaattt tttcatttga ttttacccct tttcattttt tattgtcatt attgcatgaa
     1861 aattattcgt aagattgcca attacataaa cgattgtttt aagatcataa tcgttgttgt
     1921 cttaaattca ttcctccttt ccaattcaag agtacctatc aataatgaca catactcttg
     1981 aatctttcgc caaatcataa acttgaatat cttggtaaga attcatcacc catacgtttg
     2041 ttttctctat ctatatggtt ctgtatacct acaacatcat gagcgaaatt
//
LOCUS       DACDIG010000163         2026 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.169, whole genome shotgun sequence.
ACCESSION   DACDIG010000163 DACDIG010000000
VERSION     DACDIG010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.169"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            398..988
                     /locus_tag="IV247_002877"
     CDS             398..988
                     /locus_tag="IV247_002877"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778870.1"
                     /translation="MNITISINKEFGELTEKKINRIAQSFNDIVNVQNIDSQIAFFQF
                     SDHALVLNRPNNEIAVAFSSKGLVNVSFKKLTILLDLLDLDNYGRTNISIEDIIDQEF
                     SVAEKSLDRLGINDSEIDGVGLRILVDTFGDNPKEFKIEPRIDDFSKLYIFCNQSQTK
                     VAELTEEFIDDSVKYLLDKSDEYKTFYLEKGGISGQ"
     gene            978..1145
                     /locus_tag="IV247_002878"
     CDS             978..1145
                     /locus_tag="IV247_002878"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778871.1"
                     /translation="MANKELVLIKEKKKRERAKRLEVIKSTGRIISVTTLVPFFQDDI
                     NVKEGEKKYDI"
     gene            1135..1809
                     /locus_tag="IV247_002879"
     CDS             1135..1809
                     /locus_tag="IV247_002879"
                     /inference="COORDINATES: protein motif:HMM:NF013970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HAP8778872.1"
                     /translation="MTFNPSLPFDISNKNWSDSNLFPNEMYLDTNLALMLVLRKPNFY
                     KVENFLKEYAITRGNDVFWSVLTESELFECIHVDTLKSNAKKFGYQPAKWKDMENELD
                     QKSFSMINNEADDRFTSAINVLKQYGEVLDDVNPSNQPDSRIIIKENARKIYTTYGGG
                     IKDAEHIAIANEYGINNILTNDSNSGNGFFRYPQQNIYGISKFISNGYNPNVKPNEYK
                     DLIKEI"
BASE COUNT          760 a          236 c          334 g          696 t
ORIGIN      
        1 atgaggggta gtacatatag ttttcaatat agttgctaat attgttagct aatttgtaag
       61 caaaaatgtt tacgaattta gatagtaaaa aataggattg ttcttagcat acaggaattt
      121 ctaatattgc gttactatct aaatcttctg tccaattagc taataaaatt agctaataat
      181 ttagctcata tatattgact ttcctttgtg actgtcagat aattttagtg taaggagaat
      241 ataaacgttt ttaaggtaac tcctttttgt taggttttat ctaccatcac aataaaatag
      301 atggatatat gcataataaa ctcattatgt tttttagtat aagtatataa atccactaat
      361 ttttatatgc tcaattatag cctggaggaa tagtagcatg aatattacga ttagcataaa
      421 taaagaattt ggagagctga cagaaaaaaa aatcaataga attgctcaaa gttttaatga
      481 tattgtaaat gtgcaaaata ttgattctca aattgctttt tttcaattta gcgatcatgc
      541 tttagtattg aatcgtccta ataatgaaat tgctgtagcc ttttcttcga aaggtctagt
      601 aaatgtttcc tttaagaaat taactatttt gctagatttg ttagaccttg acaattatgg
      661 aagaaccaat atttctatag aagacattat agatcaagag ttttctgtag ctgaaaaaag
      721 cttagataga ttaggaataa acgacagcga aatagatgga gttggtttga gaatcttagt
      781 ggatactttt ggggataatc cgaaagaatt taaaattgaa ccccgcattg atgattttag
      841 taaattatat atattttgta accagtcaca aactaaagtt gcagaattaa cagaggaatt
      901 tatagatgat tcagtgaaat atttgttaga taaatctgat gaatacaaaa cattttattt
      961 agaaaaaggt ggtattagtg gccaataaag aacttgttct aattaaagaa aagaaaaaaa
     1021 gagaaagagc caaaagatta gaagtaatta aatcgactgg taggattata agcgtaacca
     1081 ctctagttcc tttttttcaa gatgatatta atgtaaaaga gggagagaaa aaatatgaca
     1141 tttaatcctt ctttgccctt tgacatatca aataaaaatt ggtccgatag caatttattt
     1201 ccgaatgaaa tgtatttaga tactaatctc gctttaatgt tagtgttgag aaagcctaat
     1261 ttttataaag ttgaaaactt tcttaaagag tacgcaatta caagaggaaa tgatgtattt
     1321 tggtctgtcc tgactgaaag tgaattattt gaatgtatac atgttgatac tttaaaaagt
     1381 aatgcgaaga agtttggata tcaaccggca aaatggaaag atatggaaaa tgaacttgat
     1441 caaaagagtt tttctatgat aaataatgaa gctgatgata gatttacttc tgctattaat
     1501 gtattaaaac aatatggtga agtactggac gatgtaaatc catctaatca acctgatagc
     1561 cgcattatta tcaaggaaaa tgctagaaaa atatatacaa cgtatggcgg tggaataaag
     1621 gatgctgaac acatagctat tgcgaatgaa tatggaatta ataatatatt aactaatgac
     1681 tctaatagtg ggaacggttt ttttagatat cctcaacaga atatttacgg aatatctaaa
     1741 ttcatatcta atggatataa tcctaacgtt aagcctaatg aatacaagga cttaattaaa
     1801 gaaatttaaa atattaattt catctagttt tttctgtaaa aattatttcc gattaatata
     1861 agtaaattga aaataaatta tcaactttta gtgtctttct agataacaaa cttatacatc
     1921 ataaccttag tgtgttatct tagtatcggg aatatgagtt attatgataa tagaatctat
     1981 tcaaggctac ctgaaaaggt agcctttttt aatctttata cctttc
//
LOCUS       DACDIG010000164         2024 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.170, whole genome shotgun sequence.
ACCESSION   DACDIG010000164 DACDIG010000000
VERSION     DACDIG010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.170"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..146)
                     /locus_tag="IV247_002880"
     CDS             complement(<1..146)
                     /locus_tag="IV247_002880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778873.1"
                     /translation="MNVGFIGAGKVGCSFGKYFQEHKIQVTGFYSKSEDSSLAASNFT
                     SSKQ"
     gene            399..1568
                     /locus_tag="IV247_002881"
     CDS             399..1568
                     /locus_tag="IV247_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8778874.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRGRKEICEDFKLV
                     YQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIYSTNLIEG
                     FNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTLESMI"
     gene            complement(1908..>2024)
                     /locus_tag="IV247_002882"
     CDS             complement(1908..>2024)
                     /locus_tag="IV247_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP8778875.1"
                     /translation="TIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT          711 a          340 c          322 g          651 t
ORIGIN      
        1 tattgtttac tagatgtaaa attcgaagct gctaatgacg agtcttcgct tttactataa
       61 aatcctgtta cttgtatttt atgttcctgg aaatacttcc cgaatgaaca acctaccttg
      121 ccggctccaa taaaaccaac gttcataatt atcacctcca taaagaagtg agactacaag
      181 tcccattctt acgatatgag cttaaaaata aaaattaaat caaatataca gtatgttttt
      241 ttcaaatatc atccatagtt aatttatcat aatatcccat attttaaaat aaaagatagc
      301 gtaaaatatt ttgtgtaaat agaaatttag ggtagaaaaa aaagtccatt ctgtaaaatt
      361 aaagttacca cacagaaatt ttataggagg actttctcat gactaaattt actatagaaa
      421 ttatgcaaac actactcaat aaaggggatt tagatgaatt atttcaggat catttagagc
      481 gagcgattaa ttcacttcta caagcagagc taactgcttt tttagattac gaaaaatatg
      541 atcgaaatgg attcaattca ggaaattccc gtaacgggaa ttattcacgt acatttaaaa
      601 cagaatatgg cgagttgaat ttaactattc ctagagatcg gaatggcgaa ttttctcaac
      661 aaacactacc agcatataag cgaaccaatg attcattaga aacgaccatt atccaattat
      721 ttcaaaaagg cattactatg gctgaaatct ccaaattaat tgaaaaaatg tatggtcacc
      781 actatacgcc acaaacaatc tccaacatga gtaaattggt ggctgaagat gttttagctt
      841 ttaaagaaag aacgttagaa gccaattatt ccgttatatt tatggatgcg acgcatatcc
      901 ctgtgaagcg acagactgtt tcgaaggaag cagtgtatat tacgattggt attcgtttgg
      961 atggaaccaa agaagttctt gggtttacta ttgccccaac tgagtctgct tatatttgga
     1021 aagaagttct tcaagatctt agaaaacgtg ggttagaaga agttttatta gtagtgacag
     1081 acggattaag ctgtattgaa gaaagtatcc atagtgtgta tccgaatgcc caatttcaac
     1141 aatgttgtgt gcatgtatct agaaatatcg ctcataaagt tcgtgttcga ggtcgaaaag
     1201 aaatttgtga ggatttcaaa ttggtttacc aagcgaattc aaaagaagag gcattggatc
     1261 acatcgactt tatgactagg aaatggaaaa agcagtatcc aagagtcgtc aatttactct
     1321 tgaatcctgc cctattaacc ttttataatt tccctcacgc catcagacga acaatttatt
     1381 cgacgaacct gattgaaggc tttaataagc agctaaaacg atatactcga agaaaagaac
     1441 aattccctaa tgaagaatct ctagagagat tcttcatttc tcaatttaat caatataacc
     1501 aaaaattttt aggtagaatt cacaagggat ttaaagaaat tcaggataca ttagagtcga
     1561 tgatttaact gtaattaaca gaatggattt tccatttaca tataattctt gacgctacca
     1621 taatctaaat aaccatcata ttcatctact acttatataa ttgagtttta ttaaactttt
     1681 taagtttcta ttctatcaaa tatttaaaca atctctgcat ccgaaaagac tacctcgtat
     1741 agttttacta acaatatttt tattagcgtg ttcaaatcct gatttcttgt atcaattatt
     1801 ttctgttcac tttcaataag aaaaaagtct ttacttccat ggataataat aagggttctg
     1861 ttgcaaagtt ttaaataaag aataaaatcc cttacggtat ctatgattta agctgggatt
     1921 cccaataata ccttgatttc agtacagacc gaaaacccga agagagtgcc ttcttttcgg
     1981 gttttcttat ataatcctcg gatggcttcc atgcctttaa tcgt
//
LOCUS       DACDIG010000165         1996 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.171, whole genome shotgun sequence.
ACCESSION   DACDIG010000165 DACDIG010000000
VERSION     DACDIG010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.171"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(248..469)
                     /locus_tag="IV247_002883"
     CDS             complement(248..469)
                     /locus_tag="IV247_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778876.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(567..812)
                     /locus_tag="IV247_002884"
     CDS             complement(567..812)
                     /locus_tag="IV247_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778877.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1052..1666
                     /locus_tag="IV247_002885"
                     /pseudo
     CDS             1052..1666
                     /locus_tag="IV247_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT          673 a          347 c          288 g          688 t
ORIGIN      
        1 tttttactat atacttcttc ccagaacttg tccgtctcct tcaaattatc tctacctctc
       61 gagttcattg ggtggtttgc ccatttatta aacatggaat cacctttctc atactcatat
      121 atttatgtcc tatttcaatt atatttatat atgcttaaat aaatttgtat cctgaaattt
      181 attgtaatat gaagctgtat tttacatata gtaaccaaag agttaataaa ataaaaagag
      241 cgaaccatta tagatcagct cttttttcat tgctgttttt aatcttatct agaatcactt
      301 caaatgtttc aaaggtattt acatctaact gacataacca aagcaagtac ttcttttctt
      361 tgtaagtcca atcaatatta cggtaagagg acattagatt atggtacctt gctctttgtt
      421 caactgaagc caattcccag cgtttttcaa atattttttc ttcttccatt attgatccct
      481 cgcaagacgc tacctcaaac ttaaataatg ggcagcgaaa gtctaattcc ttagatctaa
      541 gtatagcaaa caaatatgaa aaaaacttag aaggtagtaa aagttcttcc gtcttttgct
      601 ttgcttctat tcttaatgtg tttcgttatc ggtgccctac aaagattttc attaattaca
      661 cactcattta ttgttgtgta ttttctgaca tttctctctt gatcaattat tttaattcca
      721 tgggcatcct gatagttcat aggagaggag cggctatcag aaatagttgc acctatctct
      781 tgaacttctt ttacaacttc tggaaaatcc atccatcctg cttcagctgt caaaagtaat
      841 aatctataat atttatcttt ccttttcaat ctaagaaatc gtcaccccat tctttttctt
      901 ctattataaa ctatttaact tttagaattc aaaaaccgtt tgtctattta gagtttaccc
      961 taaatagaca aaaatataac attgaaaaat ttagagttct aacgtatgat tagggtgtat
     1021 tataaacgtt atagaaaagt agggaataaa tttgaaaatt gcttatatta gagtttcatc
     1081 catcgatcaa aatgaacaac gtcaaattga agaaatgaaa aagtttggcg ctgaaaaaat
     1141 ttttatcgaa aaacaatctg gagcgacgat tagtcatcgg ccgattttcc aggaagcttt
     1201 agattttgtc agagagcaag atatttttat tgtagaagcc attgatcgac ttggacgcaa
     1261 ttatgacgag attatcgatt cagtgaatta tctaaagaag aagaatgtgc gacttattat
     1321 caccagttta ccgatcatgg ctgaagcaat tggaaatcct ctcctagata aattcatcaa
     1381 agacttaatc atttaaatct cagccatgat tgctgaacaa gaacgtactg aatcgaaacg
     1441 acgccaggca caaggaatta aaatcgctaa agcaaacggc gtctataaag gtcgtcctaa
     1501 gttatacagt gcagacacca aagatcctca acgtcgcctt gtctatagga gtatcgttca
     1561 ggatcttgag aatggggttg ctatctcaaa gattgcaaca gactacaatg tgactaggca
     1621 aacaatttac cgaataaaaa aagaaatcga ccaactaatt gtatgatttc ttttttgaat
     1681 cacgtaatta ttaattagct ttgtcatcaa tacagacaat cattttagat tcatctagct
     1741 aagttgatta catttatgtt aaaaagaatt tagttaacat aaatataatt gatgcataaa
     1801 tcgtccttat ttaaaggcat ttaacattga caacttagta taatatatct tatgtaaact
     1861 aataagattt gaattttatt ttatacacac tcgataatag atcaatttta tggtcccccg
     1921 catttacgat tgcacaagag aaacataaag tcgacctatt agcatttctt ttcaattcaa
     1981 acattaaaaa agttta
//
LOCUS       DACDIG010000166         1948 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.172, whole genome shotgun sequence.
ACCESSION   DACDIG010000166 DACDIG010000000
VERSION     DACDIG010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.172"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            181..483
                     /locus_tag="IV247_002886"
     CDS             181..483
                     /locus_tag="IV247_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778878.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(999..1072)
                     /locus_tag="IV247_002887"
     tRNA            complement(999..1072)
                     /locus_tag="IV247_002887"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1036..1038),aa:Ile,seq:cat)
     gene            complement(1627..1941)
                     /locus_tag="IV247_002888"
     CDS             complement(1627..1941)
                     /locus_tag="IV247_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778879.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          708 a          289 c          301 g          650 t
ORIGIN      
        1 ttttataagg gtttgagcat tttttatttt cgcagatttt cgttaaatga atgaaaatgg
       61 cattcaaaat agcattcaaa aagaacactt tttaatatat atgttccatc catattatca
      121 atgatattcc tactttacaa aaagaacatt agttcgtata ctctttttga ggtgaacatt
      181 atgttgatgg aaggaaaaac acaattatgg tttaaatttg atccttcgaa tagatttgtc
      241 aaagattttt ataaggtgtg ggattcagaa gttttctttt tagcaatcga agatagctta
      301 ttaatcaatc tctattactc taataagaac tactttaaaa ttccggcaac aaaaaccaga
      361 atgaagaaag atgtgtattt tttgtttgat atcgtgactg atgtgccaga cgcacggagc
      421 gatcatcggc gttatgacta tttgaagtat actttcgttg atccagaaag gtataaagat
      481 taaagtaggc tacctaaaaa aggtagcccg gaacggattt tatcaccata cttataaaag
      541 ggatattttt taagttagta ttaagattgt gtaatatgat gatatctata ttttatagta
      601 tcagtgctat aaaatcaaaa ataagtcact aattaactac cactccaatt ataagtcttt
      661 ttcccattat tttttacagt tatatggtat gctttttaat ggcttcaaat ataaaagagt
      721 ttaaagcgta acacacttat ggggaagtgg tttttgggga acgctttaaa ctcttcttta
      781 ttattatctc acaatataac ccaaatgtct ttccatttaa aaatcaaagt aaaacttttc
      841 aaatatacag aagtataact atgtgaaaca tcctttcatt aatccataaa aggatacata
      901 aaaaagccac tcatttgagt ggcaatgaag aaaagcttta gcttgtataa tactcttcaa
      961 aaaattctaa cacagaacga ttcaattggc tacattaatg taccctgtag gactcgaacc
     1021 tacgaccgga cggttatgag ccgtctgctc tgaccaactg agctaagggt agtggttgtt
     1081 gccacataaa gccataaaca atcaaccagt agaatgcgtg gcaacaaacc tgttatcgca
     1141 tatcttggag tgtgactatt tatgagtgat agtgaagata tgcgtctcca tatatgtatg
     1201 gttgagacag ataaacatgt atgaaaaacg ttgttaaatc aacgtttttt tattttggtc
     1261 aaggagagaa aagtagagaa atacaaaatt ttttgcattg attttgcatt caaaaaaaca
     1321 ttcaaacatt tagaaatccc caatacgtat tcaggaaaat ctattttaag gtttacaaaa
     1381 agaacttttg ttcgtacact ctttttgagg tgaactttta tgctgatgga aggaaaaaca
     1441 caattatggt ttaagcatgt tccttcaaat aagtttttta attataaact ctagagaaat
     1501 taattcatcc taacataaaa taattgttca ctcaaaagtt tttctagaac aattaaaaca
     1561 gcaggcttca cattcgctat acatttgtaa gaagtaataa ttcctgtact aaaaggcata
     1621 attgctttaa ttattttttg acttctcttc aaacacaaga agtagttgtg taataattgc
     1681 agaaataagg attccaataa tagatataaa atttgacatg gaaacgtcta taacattaaa
     1741 agaataaagt gttgaagaaa gtaaactgat tgaaataacc cctccgtaaa aaatagtctg
     1801 tgaatatacc cacttttttt cagaagacgt acttcgtttg gttctaaaac ctatccctgc
     1861 atttctactc ttatcaaaaa accaaatatt tagaagtaat aatattgcta ttattaagat
     1921 aaaattactg taaaaatcca aaagctat
//
LOCUS       DACDIG010000167         1926 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.173, whole genome shotgun sequence.
ACCESSION   DACDIG010000167 DACDIG010000000
VERSION     DACDIG010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.173"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            252..839
                     /locus_tag="IV247_002889"
     CDS             252..839
                     /locus_tag="IV247_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778880.1"
                     /translation="MENNNDIRHFSIGERVTTVSELSKDYRDKEGTIISLNKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNNGSHSIEKAILVYPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITSKEAKIEDVLTVRELQSLIEYRNKQITNPEYLRKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDTFKKIYLKEIKKD"
     gene            839..1738
                     /locus_tag="IV247_002890"
     CDS             839..1738
                     /locus_tag="IV247_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAP8778881.1"
                     /translation="MWTVTLPFQPVSAPRPSARRNAADETNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQDLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            1738..>1926
                     /locus_tag="IV247_002891"
     CDS             1738..>1926
                     /locus_tag="IV247_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778882.1"
                     /translation="MNNNEKILHVLDSFEIIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQE"
BASE COUNT          769 a          244 c          382 g          531 t
ORIGIN      
        1 aaagtaacag tttcagagtt gttagaagaa ttgatcgaga atgatcaaac aataagacgt
       61 gcatttacta ataggtctat cagataacaa tataattaat aaaaaaagaa agagtgattt
      121 ggtcttagac taaatcactc tttttatata aagtttcttt acactcattc cgaataatgt
      181 taaaattctt tatacgatat atagttcctt gtcagaggaa gagataacca gcaatattgg
      241 agggataata agtggaaaac aataatgata ttagacattt tagcattggg gaaagagtta
      301 caacagtaag tgaattatca aaggattata gagataaaga gggaacgatt atttctctta
      361 ataagaatcg aagaggcgtt tggcaatatg ttgttgattt aggtgatggc gttacagttg
      421 agtgtaaaga tagtcaactg atcagaaata atggcagtca ttccattgaa aaagcaattc
      481 ttgtataccc aaaagtatca agtaagatca tgaatgactg gttgcttgga aaatttggtg
      541 agtacaataa aaaagaaatc gcagaagtga tcggcaaatc ttatccaaca gtattagggt
      601 atctaaaaaa gataacgagt aaagaagcaa aaattgagga tgttttaaca gtaagagagc
      661 ttcaatcatt aatcgagtat cggaataaac aaataacaaa tcctgagtac cttagaaaag
      721 aagatggtct attgaaacaa ttggaacaag taaataaaat ccggaagatg caaaaggtag
      781 ctcctttaag ccttgatacc tttaaaaaaa tctatttgaa agagatcaaa aaagactaat
      841 gtggactgta actttaccgt tccaacctgt atctgcacca agaccaagtg caagaaggaa
      901 tgctgctgat gaaactaatg acgtagcaga gaaaaggatt tcaacctact atgataaaaa
      961 gtatcttgat tatttgaaaa aaataaagag ctttatcgaa gatcaagatt tattagatga
     1021 tgaattttac tcgattgtaa atgatcctat gggcgcaatt atggaagtgg acttttacta
     1081 ccagatacca aaaagctata aaaaagtcta taacattatg aagacaacag cgccagattt
     1141 agataattta gtaaaaagtg ctatggatgg tatttttaac atctcagcta tacgagatag
     1201 ccatatcaca ggcttaattg cttttaaata taatgtggcg aacgaaggaa aaacagttgt
     1261 gaggatcaaa cgatattcag aatttgagtg ggatacttct gaaggattga acggagatat
     1321 atgggaagct acatttgatt ttaaaccagt agcaacgcca agaccaaagt ctaaatttag
     1381 aggaaatggg attatcactt actatccaaa agagtacaac gactatttgg aaaatatcaa
     1441 gaatacgctt aaagagaaag atttggtaaa tgatcaactg aaaaaggtaa tgaatgctcc
     1501 tatgggggtt atagctcaga tagattattt ttaccaagtg agaaaagata agaaaaagct
     1561 tgattcactg atgcgtactt cacagccgga tattgataat ttagtcaaag gaacattgga
     1621 cggaattttt aacaaggaaa taggcatcaa agatagcagg gttgtagggt taattgcttt
     1681 taaatacaat acttttgaaa agtcgaaaac aaacgtgaga ctacaggaga tgggctgatg
     1741 aataataatg aaaaaattct tcatgtactt gatagctttg aaataattca agaagaacta
     1801 aagaaatatc gggatgtttt ggaacaaaga tatgattttg tcaatcagca aaagagtaat
     1861 cacatggatt tcattctcaa tatgaatgac ttgaagaaga aattagttga acgaaaagaa
     1921 caagaa
//
LOCUS       DACDIG010000168         1898 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.174, whole genome shotgun sequence.
ACCESSION   DACDIG010000168 DACDIG010000000
VERSION     DACDIG010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.174"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            130..1454
                     /locus_tag="IV247_002892"
                     /pseudo
     CDS             130..1454
                     /locus_tag="IV247_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
     gene            1447..1794
                     /locus_tag="IV247_002893"
     CDS             1447..1794
                     /locus_tag="IV247_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778883.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFAELLKWSQI"
BASE COUNT          716 a          250 c          385 g          547 t
ORIGIN      
        1 aatcgtttaa ttaagaacag taatctcttt tagtcagact tttagtctga cttttttgta
       61 tttccaaaat ttattcttta caaaagcgaa cgtacgtttg tataattgct tgtgaatggg
      121 ggaataaaaa tgtttgatta cagtagagaa ccggtgaatg actatttttt aatcgacatg
      181 aagtctttct atgctagcgt ggaatgtatt gaacgaaatt tagacccaat gactacaccg
      241 ttaattgtca tgagtagatc ggataacact ggttcaggtc tgactctagc ttcttcacct
      301 actgcaaaga aaaaatatca tatcactaat gttagtagag ctaatgattt accttatcca
      361 ttaccaaaag aactttatat tgttccacct cgaatgaact tatatataaa aaaaatatgg
      421 aagttaatca gatttttaga aagtatgtcg cagatgaaga tttattgatt tattcgattg
      481 atgaatcaat tttaaaagtg acaaaatcct taaatttatt tactagttca gaaacaagaa
      541 gcgagaggag aagagaatta gctaaaataa tcaaagatga gattgagaaa gaaacaggac
      601 taatagcaac agtcggaata ggagacaacc cattattagc taaactcgca ctggacaatg
      661 aagcgaagag tagtaaagaa tttatagctg agtggactta tgagactgta cctgaaaaaa
      721 tatggaaaat aaacgagatg actgactttt ggggcattgg atcacgcatg aaaaaaagat
      781 tgaatcatat gggaattatg agtatagaag atttagcaaa ttggaatccc tacacaatta
      841 aaagtcagtt aggtgtaatc ggacttcagt tatatttcca tgccaatgga attgatcgta
      901 cggatattgc tattccacca gaaccaacca aagaaaagtc ttatgggaat tcacaagttc
      961 taccaaaaga ttatacgagg agagaagaag tagaactagt agtcaaggag atggcagagc
     1021 aagtagccat tcgaattaga caacataatt gcaaaactgg ttgtgtacgc ttagcaattg
     1081 gtacgtcaat tttggaagaa aaatcaggtt tctcccacca gatgaaaata ccaattacag
     1141 ataatacgaa agaattacaa aattattgtc tttttttatt caataagttt tatgaggggc
     1201 aggaagtaag acatatagga gtaacttata gcagattagt atacacagat tccctacaat
     1261 tagatttatt tagtgatcca agaaaaaaga ttgctgaaga aaatttggat aggattattg
     1321 ataagattag acaaaaatat ggatttacag ctattataca tgcttcatct atgttagaag
     1381 gtgcaagaag tattacgaga agcactttaa taggaggaca cgcaggagga ttaggaggtt
     1441 tgcataatga ttgaatataa cgatagagga cgtttaaaat ggtcgggatt ttatttgtct
     1501 gatcatactg aaagaatcaa tgcagacaat gtacaacgtg ctaatcataa tttggcaaaa
     1561 gaacaaatga cgactgaaga gattgcgaaa atactgagaa ctgctgtcat aaaaaataga
     1621 cccgtctgca ttcaaaaaga ggaacgaaac gctgaaggtt actatccaga tgatgtagta
     1681 ggaaaaatta ttggatatga cgagcttggt gtatttgtgg aagaagaaaa agttcactat
     1741 gatgaaatta ggaatgtttc tttcgcggaa ttattaaaat ggagtcagat atgaacatta
     1801 ttaaaaaata cgaattcggt tatattacgt atgaacagct aatagaggaa ttatggggat
     1861 atggtcaaca attaattaat gaagtgggga ttgattgc
//
LOCUS       DACDIG010000169         1883 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.175, whole genome shotgun sequence.
ACCESSION   DACDIG010000169 DACDIG010000000
VERSION     DACDIG010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.175"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1590)
                     /locus_tag="IV247_002894"
     CDS             complement(<1..1590)
                     /locus_tag="IV247_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP8778884.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKSEDKENEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQL
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFK
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKS"
     gene            complement(1632..>1883)
                     /locus_tag="IV247_002895"
     CDS             complement(1632..>1883)
                     /locus_tag="IV247_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HAP8778885.1"
                     /translation="LNASNTNIKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMET
                     EIKAINDTQKLIDQYKNADFNKDSFGDMKTTFKYLTLTD"
BASE COUNT          513 a          390 c          295 g          685 t
ORIGIN      
        1 actcttcaac atttcattat aagcaatttc cagaagagtt tgttcggata aagcccaaaa
       61 tggaactatt cgtccaaatc ggttaataac ttcaggcttt agaccttcac caatcaaggc
      121 ttgtttgatc cctgatgcga cttttcggta ttctcttttt agttcagtgc tatattcatt
      181 agaattttcc ttgtttgtaa cacgagatag tgagctatat ctgtttaacg aatcgaacat
      241 ctccgcacct gcatttgttg tcgcaaataa aattgtattt gacaatgaaa cagtgtcgtg
      301 aacttctcga tcatcccatc ctctttcagt aaaacttatc ctaccttcat ctaaaattga
      361 taaaaaggca tcgagtgttt ctacaccagc tttctcatat tcatcacaca caataatcgc
      421 agaaggatca tgccttacat gagtacctat gagtcgtcta aaacgcattg catcatcttt
      481 tgttttgtag tccggcatat taactaagag aactttattt tcatcgccat aaagtcctct
      541 tgccacggct ttagccattg cagttttacc gacccctgtt gatcctgtaa acagagcagt
      601 tgcttttggt tgctttcgac ctttaaaacg atcaaatcca agcttcatac gtttaaacaa
      661 cattgttata gtataaactg ctaacggttg cccttttatg gactttaatt cctcttgaag
      721 tttttccata tcaaaatcag aatctagctg aatattatac ttaccaaata tttgcaatag
      781 actttctcga ttataagcat ttccgtctac tcgatctttt gcaatcaaat cctccattac
      841 ggcaattgat ttagccggtt cggacaaatc ctcacgataa agcttgtttg cttgtacgat
      901 ttcttgaagt aattcatcag gaatcctttg cgataattta aaagaagcct gaggatcatc
      961 tttaacagag gatgtattta gccattgtct caaaatatcg acgacaacat cgctttttag
     1021 ttcattcaat tctaacggtt gtaaccgtct ccctaaggca tcatctcttt tgatatagtt
     1081 ttcatattct tttcttgtag tagcggtaat caattgaagt gattcatttg ctaacggttc
     1141 ttttagcgaa tcacccccaa ttttattgcc actaccaaaa gagctaacaa ctttatgaaa
     1201 ctcgtcaatg aagaggacaa attcattttc tttgtcctca gattttaaaa ttcgctcata
     1261 ttctttcaat cttggaatta atgtttctaa acgtgtttgg aggacatcat ctcccccaac
     1321 acgcaattgt ccgattgata atttgtatac ttgtactttc tttccatctt gtcgctgtct
     1381 ttcagcccat tcacttgcaa gtgctgtttt acctgttccg gcttcaccta acaagatagc
     1441 ttgcggtgtt tccggtctat tcaagatcag tgacaactca tggagttcgt catcacgtcc
     1501 cactaactta tgtttcggtg gtttcattgg tgtgagagca tgattaaatt cagtttgaat
     1561 attcttaaaa aactcggtac tattttccat ctgtttgtgt tcacttcctc taaaataaga
     1621 ataatttact tctagtcagt cagagttagg tacttaaatg tcgtcttcat gtctccaaaa
     1681 gaatctttgt taaagtcagc atttttgtat tgatcaatca atttttgtgt atcattgatt
     1741 gctttaattt cggtctccat tcgactgtct agttgacgaa tactttcttg cgaatcttta
     1801 tcgtcaggat tttgttgtaa acgttccaga tactcagctt ttgtagcttc aattttcttg
     1861 atgttagtat ttgaagcatt taa
//
LOCUS       DACDIG010000170         1883 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.176, whole genome shotgun sequence.
ACCESSION   DACDIG010000170 DACDIG010000000
VERSION     DACDIG010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.176"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..153
                     /locus_tag="IV247_002896"
     CDS             <1..153
                     /locus_tag="IV247_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8778886.1"
                     /translation="VGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWY
                     LNIPKR"
     gene            268..1476
                     /locus_tag="IV247_002897"
     CDS             268..1476
                     /locus_tag="IV247_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778887.1"
                     /translation="MTAEIGIMNKSGIVLASDSASTIGDSKVYNTAKKLFTLDSMHSV
                     GIMIYGNAEFNGIPWEIIITQYKKSIGSSVFNTLEEYADNFIEFVKTASFIRSEQTEQ
                     EQMIGVFQKIISGLFESIEVDINFLISQGTQIDKDVLVKLLQTKMNTNLSQQSQTFIL
                     DIEKALFLSNYGEILKDILNSISTMEGVSEAISEEIQSYVYEIIIRDDVYSSPTGIVI
                     AGYGRMDIFPKLYSYNMFGFVMNILKYSEYESAQIGNDNGSLRSTILPFAQSDVVNTV
                     VQGVDPQITNYLSSQVDSFDDNGKNTYINIIKNISEFQQNQFIFPLLNMIALLPVEET
                     AIIAETLLNLTSFKRKYTTSVETVGGPIDVLAITPNDGPIWIKRKHYFDIDNNIGYRL
                     RKERANDYNN"
BASE COUNT          708 a          244 c          316 g          615 t
ORIGIN      
        1 gttggctatc atgtatcttt taacatgcgc aatttcccta ataagaaagt atacgtcatg
       61 atgcgggcaa cgaatgatcc agaaggaaac actaaaggcg gtgcgcaaga tttccatgac
      121 aaacgttggt atttaaatat tcctaaacga taaaaatagc tcctcgttga ggagcagtac
      181 atagaattga aaattaacgt taaatcaaaa aaatatttac aagatgttta tttaagtaga
      241 taataaaaga aaaaagcagg tgaaattgtg acagcagaaa ttggtattat gaataaaagt
      301 ggaatcgtgt tagcttcaga ttcagcatct acgattggag atagcaaagt atataatact
      361 gcaaaaaaat tatttacttt ggattccatg cattctgtag gtatcatgat atatggtaat
      421 gctgaattta atggtattcc ttgggaaata atcattactc aatataagaa aagcattggt
      481 agttctgttt ttaatacttt ggaagaatac gcggataatt ttatagaatt tgtcaaaaca
      541 gcatctttta ttagaagtga acagaccgag caagaacaaa tgattggggt ttttcaaaaa
      601 ataatttcag gtttatttga gagtatagaa gtagatatta actttttaat aagccaaggt
      661 acacaaatag acaaagatgt cctagtgaag ttattacaaa caaaaatgaa taccaatttg
      721 tctcaacagt cccagacatt cattttagat attgaaaagg cgttatttct tagtaactat
      781 ggagaaatat taaaagatat tcttaatagt atttctacaa tggaaggtgt ttcagaagct
      841 atatcagagg aaattcaaag ttacgtttat gaaattataa ttagagacga tgtttattct
      901 tcccctacag gaatagttat agcaggatat ggaagaatgg atattttccc taaactatat
      961 tcttataata tgtttggatt tgttatgaat attctgaaat actcagaata tgaaagtgct
     1021 caaataggaa atgacaatgg ttcattaaga tctactatat taccatttgc tcagtctgat
     1081 gtggtaaata ctgtagttca aggggtagat ccacaaatta ctaattatct gtcgagtcaa
     1141 gtcgatagtt ttgacgataa cggaaaaaat acctatatta atattataaa gaatatttca
     1201 gagtttcaac aaaatcaatt catatttccg ttactaaaca tgattgcttt actcccagtt
     1261 gaagaaacag caattatcgc agagacttta ttaaatttaa ccagttttaa acgaaaatat
     1321 actacttctg tagaaacagt aggtggccca attgatgttt tagctattac acctaatgat
     1381 ggccctattt ggattaaaag aaaacattat tttgatattg ataataatat tggatacaga
     1441 ttgcgaaagg agagagcgaa tgattacaat aattaaacct caggaaaaat cccctgtttt
     1501 gaaatttaat gagagcaatc tagcccataa taaaatgagt ataactgata cagtatttga
     1561 tgtaatggtt gagcaacaaa aaaaaattaa taataacaat agacaaagaa aagaaattaa
     1621 ccaatattaa tgttttttct gttgcgtttt ttgcagtcat aatttaaaac ctcgctcaat
     1681 cttgggcgag gtcttttttt atttttctga aaagttaaag taaataatag cataaaataa
     1741 tatattttac aaagaataag tacaatctag ttttttgcta ttaaatgtgt aataataaat
     1801 atgccatcac aacatgaaga atgaaatcca ttattatcta gtctagtcca ttctttttgt
     1861 ttgcagtatt tgtgatggct ttc
//
LOCUS       DACDIG010000171         1845 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.177, whole genome shotgun sequence.
ACCESSION   DACDIG010000171 DACDIG010000000
VERSION     DACDIG010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.177"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..237
                     /locus_tag="IV247_002898"
     CDS             <1..237
                     /locus_tag="IV247_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP8778888.1"
                     /translation="VRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTK
                     IKAKKGNKIAKLEFTFSSLTSDLPKISLRNWLEE"
     gene            complement(374..790)
                     /locus_tag="IV247_002899"
     CDS             complement(374..790)
                     /locus_tag="IV247_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778889.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(793..1077)
                     /locus_tag="IV247_002900"
     CDS             complement(793..1077)
                     /locus_tag="IV247_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778890.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            complement(1777..>1845)
                     /locus_tag="IV247_002901"
     CDS             complement(1777..>1845)
                     /locus_tag="IV247_002901"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP8778891.1"
                     /translation="CSLLVGLLSALFSYWLNNRNKK"
BASE COUNT          599 a          291 c          331 g          624 t
ORIGIN      
        1 gttcgtattg aagagtttag gagcttaatg gatatcccag aatactatca aatgggtaac
       61 attgatcaaa aagtattaaa tcctgcatta cgagaactaa aaaaatactt caatgatctt
      121 aaagtcacaa aaataaaagc aaaaaagggc aataaaatcg ccaaattaga atttactttt
      181 tcaagcctaa cgtcagattt gccaaagatt tctctgcgta actggttaga agaataaata
      241 aaaaagaact cgcccccctt acattaagca agtagacaag tcccttacaa ccgactaaga
      301 ttgctagtac attctagcat attgtatggg gcttctaaat aaaaaacaga taggaataac
      361 tcctatctgt tttttatcgt tttagaaaat ttgttgggtc gaaatttcta gaagtgactg
      421 tttgactggt ttctttttga tgattctttg gtcgatttcg atttgatgga tttagtttag
      481 cttcgatttt ttctttcact tcttgatata ctttagaatt tgtgccaata agaacgcctg
      541 tatcttttag atataaatca gcaaattctg cgatgatttc agtcatgatg gaaatttctt
      601 ttcccatagc atttagtttt gcctgattgc tggtagtggt cgtgttggta tcgggttcat
      661 aactcataca taaaaagcga atggcttccg agtagttatt gatatctgga tttttttcga
      721 tgatggaatc taaaacgtca acatgactag ggtacaaagt aacatgtttt tggacagaag
      781 tttttctcat ggttatatca ttcctccgat aagcatttca atgatttgat tatttgtgta
      841 gacatactcg cttaatattt gagtattttc tgtttggata gcaataaatt tatcgagcaa
      901 taaatcgaat tggtcttttt tgtaagtcgt aagttgacgg tcaaaatcac tttcgattaa
      961 aacttttaga tatttatttc gactccattt ttctcctgtt ttattcgtga gttcttggca
     1021 acgtttgtct attgttgcaa caaactttgg atcaatattc cgaactaaaa tgtccataaa
     1081 aaatctcctt ttgcaattat gtataattta tgtataccat acaaatacta tatttgtata
     1141 gctgtttttt gccaaaatgc tatacataag ctacccacga tggcgaaaaa gtgatatcaa
     1201 atttttttct gagaaacgtg ataggcacag cctatctacc cacttttttt agaaagtggg
     1261 tacacgtttc cttatgctct ttaaaattcg ggggggagtg ctgcacgtgt aatcgataac
     1321 caaatttaac agacgaaaaa ttgttggaat ataaaaatat gacacccgaa agaaaacgat
     1381 cgttggaaaa attaatgcaa ctagtgaacg aagacggcgg aatttcagac gcagcttttg
     1441 acgcatggag cgaatttcat ggagaatata attaagtggg tttaaatggc tctaaaatcg
     1501 cttctaagag ttttttagct tggaccaatg aaatacacca aaaactaaat aaaacgtctt
     1561 aaatcgaaaa atacgagctc tcagagcgac tttatttgta aatgttgcat ataagaaata
     1621 attacggatt ttagactttt gtaggatatt tttgaaataa atatttattt ctgttataat
     1681 ttgattgcaa ataagtgaac acgaaacaca cgaaatcccc agagaggtcg gattctctgg
     1741 ggcttttttt gtgggctgag tcgccttaat taagttttac ttcttgtttc ggttattcaa
     1801 ccagtaactg aacaaagcgc ttaaaagccc aacaagaagg gagca
//
LOCUS       DACDIG010000172         1781 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.178, whole genome shotgun sequence.
ACCESSION   DACDIG010000172 DACDIG010000000
VERSION     DACDIG010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.178"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            15..1778
                     /locus_tag="IV247_002902"
     CDS             15..1778
                     /locus_tag="IV247_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778892.1"
                     /translation="MLFLTGIAYFFYFIFSPADSNQMKEIKDSWVFYTKDDPDFKFDS
                     KYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEES
                     NPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQV
                     LTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALGFEAVSEDILSGILFEP
                     FVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAEL
                     PELMPIANIASVISTLVTSLACIGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGI
                     YQTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQH
                     IQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLI
                     LARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHSFRETHAIENPLYRKI
                     YLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQ
                     EKDTFIDRITVQLSRNYPNPV"
BASE COUNT          576 a          335 c          301 g          569 t
ORIGIN      
        1 tcattatatt atcattgtta tttttaacag gaattgctta tttcttttat tttatttttt
       61 ctcctgcgga cagcaatcaa atgaaagaaa taaaagattc gtgggttttc tatacaaaag
      121 atgatcctga ttttaagttt gacagcaagt acactcgtac gattcctacc gtggataaag
      181 acgaaacatt cattatggaa acgacacttg aaaaaccgtt ggacgaagca aatttactaa
      241 tcaaaggaaa ccatcaatgg attactgtat atctaggcga aaagatttta taccaacgga
      301 aagattatca ggaagaaagc aatcccggac tctcattggc tgtgatagat ttaccagcaa
      361 attatatcgg aaaaaaattg acccttacag tctcatctcc ttatcagaac tatgctggac
      421 tgccaccgaa agtttatcta ggaacaactg gaccattgat tagttttatt tattcacaat
      481 cagtgccgca agtattgacg atgataatcg ctgtttttat cgctataggg acctttttct
      541 atacgatcta cttgatgtat aaacaaaaac gtcttgaata ctcacttatt ctcttaagtt
      601 gctttgcttt ggcattagga tttgaagctg tatcagaaga tattttaagt ggtattcttt
      661 ttgaaccatt cgttcattct tttttatctc atttatttat tatcttaacg tcagctttca
      721 tcatctgcta ctattggagc cgaatgtttt actataaaaa atggtacgga attttttgta
      781 cgttccaact tttgattttt atcggcgttc tgctttatgc aacttttact cctgcagaac
      841 ttcctgaatt aatgccgatt gctaatattg ctagtgttat aagcacttta gtcacttcac
      901 ttgcatgcat tggcgaagca tataagaata accgttttta tgtagcttgt acaccttgga
      961 tcgtcttggt agccattatc cattgcttga tttacatcca aacggcatta ggtatctatc
     1021 aaacaacgat caattggtct acgattttat atattatcat tctaatcgtt attatcagct
     1081 acaatctaat cgatcacatc ttaaataccg acaaagatcg gagacaagta gatttcttaa
     1141 aaatcaaaaa tgatttgttg gagcaacatt atgagcagct tcgacagcat atccaagaag
     1201 taaatacact tcgcctggaa tttgtccaag agatggagaa tctgcaagat ttaatgcata
     1261 cggaccaaac gaaagctaaa aactatgtgg aaaagatatt agaagaagca aaaaatttcg
     1321 aaatgctgtg ttctttttgc aaccaccagg tgacaaatct tatcttagcg cgttaccaga
     1381 agttagctgc caaacggaat attcagataa ccttccaagc agatcttccc gaagaacttc
     1441 ctatcaacga tgacgatctg gctcaacttt tgatccacgc tttagagcat tcctttagag
     1501 aaactcatgc catcgaaaat ccactgtatc gaaaaattta tttgtcgata cacgaacaag
     1561 aggaacattt cgtcatttgt tgcgaacata gtgcacatta cgatacaaac ctatttagtc
     1621 gaggcatcac agaagaatta gatgatcagg aacgttttga cttaatgatg atgaaagacg
     1681 tagcggatcg ttacacagga acactaacgc aggaaaaaga tactttcatt gatcggatca
     1741 ctgttcaact ctctcgaaat tacccgaatc ctgtataaaa a
//
LOCUS       DACDIG010000173         1771 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.179, whole genome shotgun sequence.
ACCESSION   DACDIG010000173 DACDIG010000000
VERSION     DACDIG010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.179"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(128..346)
                     /locus_tag="IV247_002903"
                     /pseudo
     CDS             complement(128..346)
                     /locus_tag="IV247_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(612..806)
                     /locus_tag="IV247_002904"
     CDS             complement(612..806)
                     /locus_tag="IV247_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778893.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
BASE COUNT          615 a          266 c          242 g          648 t
ORIGIN      
        1 tcccaagaag ctcccacttc aagcgttaag aaccgtcagg tttagctaag tggtgagtag
       61 ttcacgtagg tatttttatt atatatagat ttaacatatg tcggtcggtt gattgatata
      121 gctaaagtta taactcaacc gaagccgatt catctacacg aataaagttg cgagtgttct
      181 cggctaattc ataaaaattg ttatgtgttc catatagcaa tactggaata actttatttt
      241 cttgaatctc ataagcaact ctatacacgg tgaccgcata cttaaatcca ttagaccaat
      301 tacctgataa acctccagtt ttttgctctt acttaaatgg atcaatggca atttcatcac
      361 aaacctatag aggatgagtg acttctgcct aattacgtta aaaaatattg aaagtaactc
      421 tatgtagcaa aaaatatcta gaatttcatt tcctcaaaac tgatcgatat tgtatctaaa
      481 aatatatact acctaaaata ggtagtatat attaaaatat gtgcttttgt tagaaaaatc
      541 aatacataaa ttatcatatc tgtatatgtc atttaggtat ttcagttatg ttataatagt
      601 tttgctgtct gctaccccgg cctaatctgg caaggggagg aggagatata atgaaatacc
      661 tttttgattt tgtttttgca atcaagccac ttgcagatgt agtgtgcagt tacctctgca
      721 aatggcttga ttgcaaaatt gccaaaaaag acagtaagcc tacccgttaa ttctataata
      781 tagaaacgta aaaaaaaccc catcagcctg cacgctgatg gggtttggtg atataatgaa
      841 atacctcttt gttactcata attataccat aattagtcac gaatgcaacc aatagaaaaa
      901 actacttaga acggtagatc atcgctaata acaatgcatt gtccaccaaa tggattttgt
      961 gaagcattaa atacattgtt attaattgtc gctgctttta actaggcctc tccccttcta
     1021 ttataaaaga agatatatat catttcttaa ttacctacta ccttaaacca aaatttaagt
     1081 actacctaat ttaagtagta cttaaacaat ctaaaaaata gttatcccaa ttgttatgct
     1141 tttataatac gattatttgt aacaaaatga atattatata attaaacact accatattta
     1201 ggtagtgttt aattattagc aaaacaaaaa atatttattc tattctctta acttcaacta
     1261 agttatgctg cacaccttta tataaatcat atgcaactca ttagctagac aaactacaag
     1321 catgtagttt tatctgttaa ttatgtacta cctaaataaa gtagtttata attagcaagt
     1381 atatatattt aaaaatttaa gattaatttt tttatgactt gtacaaattt atctgatatt
     1441 ctcttattaa gaactaccta atttgggtag ttcttatttt ttacatttta agtagtattt
     1501 aattgcaatt tggtagtatt tttttattat cttattctta gaacggtagt acttaatata
     1561 aatataggta gtatctaatt taagtattaa tggtagtatc taaattaaca ggtagtatgt
     1621 aactacttta aagtaatttt tttcagatta acacagtagt atcttattaa ataagttaat
     1681 tttaatggta gtacataatt attatatata tctgacattg gtagtactta ttgtattttt
     1741 tagtagtata taatctattg ttttctagcc t
//
LOCUS       DACDIG010000174         1750 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.180, whole genome shotgun sequence.
ACCESSION   DACDIG010000174 DACDIG010000000
VERSION     DACDIG010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.180"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..412
                     /locus_tag="IV247_002905"
     CDS             <1..412
                     /locus_tag="IV247_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP8778894.1"
                     /translation="PDWSTSPFNGIYQGQCTELTWAYMSQLYDGNQPTTGNGGYVWES
                     YKSAGAKITNKPIVGYGFSASDGWLWAVGDAGHTGVVIAVFEDGSFLCANFNVPPTQA
                     PTRGVSVTLIDGVDGSDNVHFFSGVGKAKFEIEK"
     gene            435..962
                     /locus_tag="IV247_002906"
     CDS             435..962
                     /locus_tag="IV247_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778895.1"
                     /translation="MNSKVKDTIIISLLSGMLLALLVGILFFNKQNQDIEAEKHSVHI
                     EDKLTFFKEVKPNEVDGLRKDLENDIQEMLNGSVQNKFADQSGEEFLRKLFIVTGKPP
                     ITDKSSEKEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            949..1512
                     /locus_tag="IV247_002907"
     CDS             949..1512
                     /locus_tag="IV247_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778896.1"
                     /translation="MQNHEITTAKKAIGILSIIVILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLEDLDSYVRAE"
BASE COUNT          671 a          238 c          358 g          483 t
ORIGIN      
        1 accagattgg tcaacttcac cttttaacgg aatatatcaa ggtcagtgta cagagttgac
       61 gtgggcttat atgagccaat tatacgatgg gaatcaaccg actactggaa atggtggtta
      121 tgtatgggaa agctacaaat cggcaggagc aaaaataacg aacaaaccaa tcgtaggtta
      181 tgggtttagt gcttcggatg gatggttatg ggctgttgga gatgcagggc atactggtgt
      241 agttatagcg gtgtttgaag atggatcatt tctatgtgct aactttaatg ttccaccaac
      301 ccaagctcca acaaggggag tttcggtaac gttgattgat ggagtggacg gatcagataa
      361 tgtgcatttc tttagcggtg taggaaaagc taaatttgaa atcgaaaaat aaattggaat
      421 gaaggacgat atagatgaat agtaaagtga aagatacaat tatcatttct ttgctttctg
      481 gtatgttatt agcactgctt gttggaatcc tattcttcaa taagcagaat caggacatag
      541 aagctgaaaa acacagtgtg catattgaag ataagctgac attctttaaa gaggttaaac
      601 caaatgaggt ggatggatta cggaaagatt tagaaaatga tattcaagaa atgttgaatg
      661 gatcagttca aaataagttt gcagatcaat caggtgaaga atttttgaga aaactattta
      721 ttgtaacagg taaaccacca attactgata agagtagtga gaaagaaaaa atcgagtact
      781 ataagccttt tacttttaag ctaacaaact ttggaattaa gacatctagt agtgggttgt
      841 atgaagcatt gaccgatttt gaagttcaat acaatggaaa aaaagtgtat gataccacat
      901 taaccatcac actaaataat gataagaaag tgactggggg tgctttttat gcaaaatcat
      961 gaaatcacta ctgctaaaaa ggcgatcgga atactttcca taattgtgat cctttgttgt
     1021 gtggtactat ttcaaaagaa aaatgcttat gataataatg tcaaagaatt ggaagacctt
     1081 aaaagtaaac aaatcgaagt agtcaagaat aaccagaaag tgaaagctat gcaggaagct
     1141 caatacaaag aattgggaaa tgctgaaatt aaggaacaaa gtaggaaatt ctttaattta
     1201 ttttacaact ggaattcatg ggaaaaatat acgaagaata tgaaggaaat acaaaaagaa
     1261 tttcctcaga tagtaaagtc tagtcctgtt gatatttctg gcacgatggt aggcactggt
     1321 aatagtccaa tatctagcta tactgctgat tactatacaa caaatcaaaa aggtaaaatg
     1381 gttgaattga ttacacagac aagaaatact ccaacaacag caacaacaac aatttggcgt
     1441 ggagtaacaa gcataggaga aaatcaattg ttttatttgg aggatttaga tagctatgta
     1501 agagcagagt aaaataaaaa aagagctaac ggtaccaacg ttagctcttt gtgctgacga
     1561 tgataacaaa acctaccgaa aaaggattgt ttcatcacaa acacaaacag aaaagaattg
     1621 ataaaaaacc cgaacctcag aatttttatt aatcaattca tttagattat aataaattga
     1681 gctgaacatt acaatacgta tatgttgtaa aacgtaaaaa aagtcaaaag taaatacttt
     1741 cttaaaaagt
//
LOCUS       DACDIG010000175         1676 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.181, whole genome shotgun sequence.
ACCESSION   DACDIG010000175 DACDIG010000000
VERSION     DACDIG010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.181"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(1..355)
                     /locus_tag="IV247_002908"
                     /pseudo
     CDS             complement(1..355)
                     /locus_tag="IV247_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            630..1172
                     /locus_tag="IV247_002909"
     CDS             630..1172
                     /locus_tag="IV247_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778897.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            1262..>1676
                     /locus_tag="IV247_002910"
     CDS             1262..>1676
                     /locus_tag="IV247_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAP8778898.1"
                     /translation="MDELKRTEIQIIDKKERPKNGFVLSEKTDLENTEAFNKESVKDL
                     RKLPTKDINGKDFTSKDFEKYDLTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIV
                     GVVTDPVDDNGENKEAIEKSKLIHEKTKASYPFLMP"
BASE COUNT          612 a          221 c          319 g          524 t
ORIGIN      
        1 tgacgagaaa tatttagaat tttgcacatc gctgatatgg aatatttatg cttgttggca
       61 tcaattactt gtcttttcgt ccgaatatca gcgccgcttg ctttaaaata tcattctcca
      121 tttcgagttg cttatttttc tttcttagtg cgatcaattc tgcttgttct ggtgttaagt
      181 tgtctctttc tttgaatgag cccgttgatt gtgcttgctt catccattta tcgaaagaag
      241 aaggcgtaag ctcatactct cgaataattt ctgcgcgagg cttaccagcg agatagagat
      301 cgacgatttg ttgtttgaat tcttttgaat aggttcttcg ttgacgtctt gacatgaaaa
      361 atcctccagt gtgtttttta ttattctaca caccttattt tttctgtcta gtctagtgta
      421 gccgattcaa attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg
      481 tccgtacact tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac
      541 aactttaagc ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt
      601 tcatcaacgt ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt
      661 gtgtaaaaaa taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat
      721 tacttgcatt atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata
      781 aaataaaaga aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg
      841 gcaaaaatga aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt
      901 atgaatattt agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata
      961 aaaagatagc gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt
     1021 tgacatttga gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg
     1081 gatataaaaa ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa
     1141 atacatcaga agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt
     1201 ttcaagtgat ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct
     1261 tctggatgaa ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa
     1321 tggttttgtg ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc
     1381 ggttaaagat ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa
     1441 agattttgag aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg
     1501 tgtaaaggaa attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa
     1561 tatcgttgga gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga
     1621 aaagtcaaag ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DACDIG010000176         1651 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.182, whole genome shotgun sequence.
ACCESSION   DACDIG010000176 DACDIG010000000
VERSION     DACDIG010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.182"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            65..865
                     /locus_tag="IV247_002911"
     CDS             65..865
                     /locus_tag="IV247_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778899.1"
                     /translation="MVGISKTALDSLAKLFSSRFDSAEFVEYLSQNESGKTLIETMKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLIGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYANDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            953..>1651
                     /locus_tag="IV247_002912"
     CDS             953..>1651
                     /locus_tag="IV247_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778900.1"
                     /translation="MRGDQTMSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWIS
                     AVGEENIEFEIKNMDDLKAKFPFIASKLSQFAEDKSWNELVSFSEKIYDITQEDECVS
                     WEMNNQYKKIDAFVKSRIEGTAFEFEGIDTAENWLNKESFIKSIADDLEKQWSKEDYA
                     EYLKTERDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYIVEEALS
                     QYSSDEEVDEETVRT"
BASE COUNT          647 a          201 c          351 g          452 t
ORIGIN      
        1 tgtagaaaat cttatttgtg aatgaaaaac aagtcatcgt aaaatattaa taggaggtgt
       61 agtaatggtg ggaatatcaa aaacagcttt agactcatta gctaaactat ttagtagcag
      121 gtttgatagt gcagaatttg ttgaatatct aagccaaaac gaaagtggaa aaactctgat
      181 cgaaacaatg aaaaaagact atttgaatga aaatgacttt ccgacatgga cgaatattga
      241 agatgctaca tgggatgtag tgtgtacgat atattctcct tgggtaacga ttgctgattt
      301 tgttttagat aaaattggag gggatacata ccctgatgcc aaatctcttg ctacaagtga
      361 ctttaaaacg ttctatggtt atctaatagg tctaataggc aatggaatag atttgaaagt
      421 tacgccaaaa caaacacctg aagaaattga aaaagaaaaa ggagaggttc aaatggctta
      481 tgcaaatgac agagaaaaag taaaattatt ggaaggtaaa ttatctgaag aagaacttgg
      541 aaaaatggtg caagaaatca atgaagacag caactatttt cctgaattga aggtatatca
      601 aaatgacgaa gatttcttta atgaaatgtt tagtaatagt ccatgggaag cagtgagtaa
      661 aacaaattta ggagaatgga actttcaaga tgatctagtt gcatttagca atgatgggta
      721 tttacgatca tattcagaaa gtgagtatag agctgaatta aaaacgtatg aaacggatat
      781 tctgatgagc tatttaaatc tattagagag tggtcaggga gagttaagag ataaagttga
      841 agaactatta aaagaagagc tatagggatt atttcaacat aaacacaagc agaaaactac
      901 caaacaaaac gtaccatgca aaaagtcggt taagccggct ttttgctcta ttatgagagg
      961 agatcaaacc atgagtgcaa caaccgatat ggctttagaa gcagtaataa aagataatac
     1021 aggaaatgtc gaaaatgtag aagactttag cgatattgca atcaatcttt cagcatggat
     1081 ttcagctgta ggagaagaaa atatcgaatt tgagatcaag aatatggatg acttaaaagc
     1141 aaaatttcct tttatcgctt cgaaactaag tcaatttgcc gaggataaat cgtggaatga
     1201 attagttagt ttttccgaaa aaatttatga tattacgcaa gaagatgagt gtgtttcatg
     1261 ggaaatgaat aatcaataca agaaaattga tgctttcgtc aaatcacgta tagaaggaac
     1321 agcttttgag tttgaaggta tagataccgc agaaaattgg ttaaataaag aatcctttat
     1381 taaaagtata gcggatgatc ttgaaaaaca atggtctaag gaagactatg cagagtacct
     1441 aaaaacagaa agagatacag aggtgaaaga aaaactcact gaggtatgtt tggatgaaca
     1501 tattgatccc aatatcgtaa aacaagaaga ctatgaagtg gttattgata aagttcctac
     1561 tgattttgag gaagttgtaa agtatatagt tgaagaagca ttatcgcaat actcttcaga
     1621 tgaggaagtg gacgaagaaa ctgttcgtac c
//
LOCUS       DACDIG010000177         1648 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.183, whole genome shotgun sequence.
ACCESSION   DACDIG010000177 DACDIG010000000
VERSION     DACDIG010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.183"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..820)
                     /locus_tag="IV247_002913"
     CDS             complement(<1..820)
                     /locus_tag="IV247_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HAP8778901.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKE"
     gene            complement(979..1245)
                     /locus_tag="IV247_002914"
     CDS             complement(979..1245)
                     /locus_tag="IV247_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8778902.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1235..1591)
                     /locus_tag="IV247_002915"
     CDS             complement(1235..1591)
                     /locus_tag="IV247_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP8778903.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          469 a          318 c          197 g          664 t
ORIGIN      
        1 cttcctttaa aattcccgat ttacttaatt tttctagtac atcaaatact ctatcactca
       61 agtaactcct taaattgtta tcactttccc attgaactac ttttctataa gttgaattat
      121 cagttgaaaa gatagaattt tcatccataa acgcatatat tagcagcaat ctttcatcat
      181 tgtcaaattc tattggatca aaaatatcta agttgtcatc aaaatcttta tcagttccgg
      241 taactttttt gttaattact gaaggtgtct tgtaagaaat agaatctaaa tcaaagcgtt
      301 ttgattctaa attattttgt gctttctcaa ttaaataatt cacttttttt gtgagaaacc
      361 ttctgatatt gtctttgcta tatgttccat aaaaaccctt atcttctaaa gagtccctgt
      421 acttcttaac ttctatatac tcttcagcca aaacttgtgg aactggttca tctaagaaaa
      481 aaacccctac ctcgcctttg ctatttttat aaaagtaatc aatttctgct atagttcccg
      541 atctataatt atcaactgga gaacccaact tatggccaaa aatcgctaat aatatatcac
      601 tattttctac aatttgttta ttaataacac tttgcccaga gctattattt atataagtag
      661 gaatcgcatt cgtttcccat cgaattggca tcaaaattac ttttgtatta tctgtatttt
      721 ctatattcca tctatatatg gtttcttcta cttcattcct ctttgctgct atatcacctg
      781 gtgatgctat aaatactttt attagattta tggttttcat ttattgtacc tttctcttta
      841 tgtctcttta aagtaatttt tgtataaaat ttattgactt ttacactata ggacaacttt
      901 tatctattta gtacaaaaag gcccactaaa ctaattgatt agttaggagc ctattacagg
      961 tatattttca actcgctttt aagcaaattc tactaccagc tttttgccta atgccctagc
     1021 gatatcatca agtttatcga aagatacgtt atcccctctc tcaattcgag caatagtaga
     1081 ttgagttgta tgtgcttttt gtgcaagctc cgcttgagtt aggcctgctt cctcccgtgc
     1141 atgataaaga gcaaccgcag tttcgagttt tcttccttca cggtcatatg cttctgcaaa
     1201 atctttgtct ttttgctttt cttcaatcaa ttttttaatc atcatttgtg aaccactcct
     1261 ctctaagttc ttttgcatgt ttaagttcac ttattggtgt tttctccgtt tttttagtaa
     1321 atccatgggt aatgacatac cttccattga ccacatggaa atagatagct ctttggatgt
     1381 ttgatcctac cttacttctt aattcgtaca aattagtgtc taattttttc acccatttca
     1441 accgttgagc tactagcatc ccattctttt ctgtttcttc tattgtacgt aaaagcttcg
     1501 cactatcttt ttttggtaat gtttccaacc attctaaaaa ttcatcatga ccattaggtc
     1561 ttttaaatgt ttcaaaagat attttttcca tacccaatta tatcatatat tgattttata
     1621 ttatagtata taacataaaa attcgaat
//
LOCUS       DACDIG010000178         1564 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.184, whole genome shotgun sequence.
ACCESSION   DACDIG010000178 DACDIG010000000
VERSION     DACDIG010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.184"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..453
                     /locus_tag="IV247_002916"
     CDS             <1..453
                     /locus_tag="IV247_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HAP8778904.1"
                     /translation="KNLVTAARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYS
                     GLVERFNENVNDYNELLEENKSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNV
                     FKGFVDKVKDKTAQFQEKHDLEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            698..>1564
                     /locus_tag="IV247_002917"
     CDS             698..>1564
                     /locus_tag="IV247_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAP8778905.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLVGFMR
                     ATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPNVKV
                     QMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLISYG
                     GLLKEIHKKLNLD"
BASE COUNT          617 a          184 c          317 g          446 t
ORIGIN      
        1 aaaaacttag tgactgctgc gagagataac gataggttaa aacagcatgt tagaaatctc
       61 atgagtactg atatggcgag agaatataaa aaattaagta aagaacatgg gcaagttaaa
      121 gaaaaatata gtggtcttgt agagcgattt aatgaaaatg taaatgatta taatgagttg
      181 cttgaagaaa acaagtcttt aaagtctaaa ataagcgatt taaagcgtga tgtgagttta
      241 atctatgaaa gcactaagga attccttaag gaacgtacag acggcttaaa agcctttaaa
      301 aacgttttta aggggtttgt agacaaggta aaggataaaa cagcacaatt ccaagaaaaa
      361 cacgatttag aacctaaaaa gaacgaattt gaactaactc ataaccgaga ggtaaaaaaa
      421 gaacgaagtc gagatcaggg aatgagttta taaaataaaa aaagcacctg aaaaggtgtc
      481 tttttttgat ggttttgaac ttgttctttc ttatcttgat acatatagaa ataacgtcat
      541 ttttatttta gttgctgaaa ggtgcgttga agtgttggta tgtatgtgtt ttaaagtatt
      601 gaaaaccctt aaaattggtt gcacagaaaa accccatctg ttaaagttat aagtgaccaa
      661 acaaataact aaatagatgg gggtttcttt taatattatg tgtcctaata gtagcattta
      721 ttcagatgaa aaatcaaggg ttttagtgga caagacaaaa agtggaaaag tgagaccatg
      781 gagagaaaag aaaatcgcta atgttgatta ctttgaactt ctgcatattc ttgaatttaa
      841 aaaggctgaa agagtaaaag attgtgctga aatattagag tataaacaaa atcgtgaaac
      901 aggcgaaaga aagttgtatc gagtgtggtt ttgtaaatcc aggctttgtc caatgtgcaa
      961 ctggaggaga gcaatgaaac atggcattca gtcacaaaag gttgttgctg aagttattaa
     1021 acaaaagcca acagttcgtt ggttgtttct cacattaaca gttaaaaatg tttatgatgg
     1081 cgaagaatta aataagagtt tgtcagatat ggctcaagga tttcgccgaa tgatgcaata
     1141 taaaaaaatt aataaaaatc ttgttggttt tatgcgtgca acggaagtga caataaataa
     1201 taaagataat tcttataatc agcacatgca tgtattggta tgtgtggaac caacttattt
     1261 taagaataca gaaaactacg tgaatcaaaa acaatggatt caattttgga aaaaggcaat
     1321 gaaattagac tatgatccaa atgtaaaagt tcaaatgatt cgaccgaaaa ataaatataa
     1381 atcggatata caatcggcaa ttgacgaaac tgcaaaatat cctgtaaagg atacggattt
     1441 tatgaccgat gatgaagaaa agaatttgaa acgtttgtct gatttggagg aaggtttaca
     1501 ccgtaaaagg ttaatctcct atggtggttt gttaaaagaa atacataaaa aattaaacct
     1561 tgat
//
LOCUS       DACDIG010000179         1561 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.185, whole genome shotgun sequence.
ACCESSION   DACDIG010000179 DACDIG010000000
VERSION     DACDIG010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.185"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            96..1151
                     /locus_tag="IV247_002918"
     CDS             96..1151
                     /locus_tag="IV247_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HAP8778906.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYQDI
                     LSFSLANLARLEDQSDDVLTVNLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDESLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            1171..1524
                     /locus_tag="IV247_002919"
     CDS             1171..1524
                     /locus_tag="IV247_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778907.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKNKPTDHNPIQQAHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
BASE COUNT          565 a          272 c          280 g          444 t
ORIGIN      
        1 atttttttct tataaacagt tgcaactatt agtaaatagg tgtaaactat tttcgtaccg
       61 aaataccata aacacaaaca gaaagaggtc ataccatgaa agaaccatta ttgttttccg
      121 ttgacttagg gaataaatca gtaaaagaaa tgagtacaca cgtttctcca ctctcgcttc
      181 cctctcgtat tttgaacaaa aatatcatta attcaggacg tttttctaat ataggttcct
      241 cattaaaacg atcactaaac gtttctgaat atagcctagt aaacactact gaaagctatt
      301 attttgggaa ggatatacac gagctaggaa aagatgacta cgtgatagaa tcactaggtt
      361 ttgggatcag cagatacaag caggaaacct accaagatat cctatctttc tctttagcta
      421 acctagcacg tttagaagat caatctgatg acgttttaac cgttaatcta gttttaggtc
      481 tgccttcatc cgactatacc gaaacaatcg tcaaatacgt gatgcaacaa gccacacgac
      541 aacattcagt tgaagttgac ggtaaagctt ataatataaa cgtagatacc gtttgggtac
      601 tgccacagcc gattggaaca ctatataacc tcctccttgc tgatgatggt agtgtacaag
      661 atgaatcatt gatcagtcag aagatcggtt ttgttgatat tggtggtggc acgaaacttt
      721 ttgatgttgt ccaaaatttt gaaattgacg aaaacaaccg tgaacaaaag gataccggta
      781 tttttacttt atataatcgt atcgtaaata tgcttgacct tccgatcagt cctaatgctt
      841 tccaacttga aaagattatc cgagaaggca aagaaaaagg cgtatatcta tttaaaccaa
      901 gcggaaatga aagttttgat atttcagaag tagttgaaag agaaataaaa acatacacca
      961 atatggttgt tgcagaagtt ttagctacat tcaaaaactt cacaacctta gataaagttg
     1021 tctttactgg tggtggggct tctattatcg acaaacaact tgtaaaagat aaaattccgc
     1081 ataccttgtt tgttgaagac agtgaatttt ctaatgtaaa aggcttttac aagtttggtt
     1141 taactcaata aaacagcgaa ggagatcaac atgaataagg gaaaatcaga gcgttatgta
     1201 gttataatca atagagaaaa ggttagcgag ttacctttag cagagtttct tgaatctgaa
     1261 tatgaaacac taggcgttaa atactctcag acgatcaaaa aagctttgaa tctatatcgg
     1321 cgtgtaaaga acaatgaagt tacaattatt gaagcaggca acgatagaaa cgatagcaaa
     1381 gtagctgaag taaagaataa accaactgat cataacccta ttcaacaagc gcatccttca
     1441 acggttacat ccgatcattc agcaaagaaa gaaaagccaa tgctaaaagg gttcaatgca
     1501 ccaagtaaca aagacttact ttaaaaataa aaaggcgagg ttacaatacc tagccttttt
     1561 a
//
LOCUS       DACDIG010000180         1534 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.186, whole genome shotgun sequence.
ACCESSION   DACDIG010000180 DACDIG010000000
VERSION     DACDIG010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.186"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(178..1197)
                     /locus_tag="IV247_002920"
     CDS             complement(178..1197)
                     /locus_tag="IV247_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8778908.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1208..1414)
                     /locus_tag="IV247_002921"
     CDS             complement(1208..1414)
                     /locus_tag="IV247_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP8778909.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          432 a          318 c          261 g          523 t
ORIGIN      
        1 acataaagcc ataaacaatc aaccagtaga atgtgtggca acaaacctgt tatcgcatag
       61 cttggagtgt gactattatg ggtgatagtg aagatatgcg ataacatcac tattttatcg
      121 aatgattttt atagttgtca atatagttat gtaatgctcc tcaacgagga gctattttta
      181 tcgtttagga atatttaaat accaacgttt gtcatggaaa tcttgcgcac cgcctttagt
      241 gtttccctct ggatcattcg ttgcccgcat catgacatag actttcttat tagggaagtt
      301 acgcatgtta aaagatacat gatagccaac atttccagaa gtattataag cttgatttac
      361 atctgatcta taaattccat cagctcttac tcgagctaat tctttcccag tattgtaatc
      421 cataatgaag atatactcgt atttatagtt agcaatgtgc catccagcta catgcaaatt
      481 tgcattttcg atttctccaa actgatcaat gtgggcgtaa ttcgttccat ctgtcagtgt
      541 aggattagct gcaccagctc gtgttggatc aatgacaggc ttgttttcag aagttgttgg
      601 attttcatcg gtaaatccat gagctaaatc atatgctaat ttttctttac ttacgcccat
      661 ttcagaaaga taaccgtaag gatctgtatg atcgccccag atgttttgtg ttacccataa
      721 atgcgatttg attcccggtt ggttataagg agtgtccaac gttaatggaa taccatattt
      781 cattgctgaa tctctagcca attcaacgta tgccttgtag tttttctcaa acgttgcttt
      841 atcatgtgtg tgttgtaact caatctgcac aggactgttg gcattagcat acgaaccagc
      901 accgtactgt acataaccag gttgaccgac ttgataaaca attccgccgt ctcccacaat
      961 ataagcagta taagcgctag tccatgaacg ttgcatatac tgcgcttcat tgcgtcctgt
     1021 tgctgtttca ttagccgttt catgcagtaa aatatactga ttatttgcta cttgtgagct
     1081 accttcattt gcgcccaaat taaattcatt gttgatagta taggcaaatc cgttaattgg
     1141 caataaaaaa agagccgtta acaggctcat cgcagtaata gtaattttct ttttcatatc
     1201 tatcctccta tttatctgat gaaattttct tatacgttcg atttgataca cctaaaatac
     1261 tccctagaaa tgctccaaaa cctgtaataa tcatcaccgt tatatctgtg taccgccagt
     1321 taacaccttt tccgacaaga ccgacaaaag tcgctaaagc ggggataact actatagcga
     1381 tccatttcaa aatatcaaag gttctattgt tcatactatc cacctccctt cattggttgg
     1441 gctactttct ttttcaatcg cttgttttct tctatcaata tttctctttc tgaaagcaag
     1501 gcatcaattt gcccgtccag ttgttcaatt ttat
//
LOCUS       DACDIG010000181         1530 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.187, whole genome shotgun sequence.
ACCESSION   DACDIG010000181 DACDIG010000000
VERSION     DACDIG010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.187"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            288..695
                     /locus_tag="IV247_002922"
     CDS             288..695
                     /locus_tag="IV247_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778910.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            709..1110
                     /locus_tag="IV247_002923"
     CDS             709..1110
                     /locus_tag="IV247_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778911.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1112..1483
                     /locus_tag="IV247_002924"
     CDS             1112..1483
                     /locus_tag="IV247_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778912.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          579 a          186 c          265 g          500 t
ORIGIN      
        1 ttgaggggaa gtacatatag ttgttgatat agttgctaat attgttagct aatttgttag
       61 taaaactgta tacgaattta gatagtaaaa aaacagtttg ttccttatat agaggcactt
      121 ttactactga ttaaatgtca gatatgctta caaatatact tataaatatg cttacaaaat
      181 tagctaatat ttccgttgac aaagctttgt tactattgtt aaactaaatt gactagcaaa
      241 agtgaaacat aaatttatta aataaatgta aaaattagga gaaatcaatg tcttatagag
      301 tacaattaat aatttcagaa gatgtagaaa gtcagcaatt tggcactaat gttattaata
      361 aagtaattaa tccagctctt tctattaatg cgcctttaat tcctactgct ttatcatttt
      421 ctgtaacagc tgtggtgtct gagatagagg atacagaaaa gataaaaata gtagaaattg
      481 aagttttaaa caagaatgaa aaacaaattt tttctacggg tgaagtatcg gtaagcttgc
      541 ctcctcaagt taacgatata aactttaata taaacgccag aaatgtcttg gttgaagaag
      601 caggagagca ttatgctgtt gttaaattta atggaactga gattggaagg caaatctttg
      661 atatcaaggt caacaaacca gtggaaaaaa attaaggaga cgatacggat gcttgatata
      721 gtaccatcaa atacacatcg gagtggtagc ttaataaatt ttgctgcgat ttttgcttgc
      781 gcatcggtaa ttctagcatc tccagttgtt gtggatgcaa gttctactcc gaaaactaaa
      841 aatgataatc aacttgtaat tactacaaaa aatgagatta acacaatttc aagtaatgat
      901 ggcaaaattt ttgatgtgat aaatacggtt ttaaaaagaa aagctgaaat caatataaaa
      961 tatgatgaag atttaaactt gtatttcttt gttatcaaaa caacatctga attgttttcc
     1021 tcagattacg atgttttgga tactctcgat aatgtattat ctgactataa atatatgggg
     1081 aaatctgtag tagcaacttt gggggaataa aatgtttgac tttaatgaat atttatctat
     1141 ttccaaacaa attgaatcag atgataaata taattctaaa gaatcttata gaagaactgc
     1201 tgtttcaaga gcatactaca gtgcttttaa aaaatctgat gagtatttga aagaaaatta
     1261 tgatattatt tataatggaa gtagcggaaa aggaagccac caaaccgttt ggaatttgtt
     1321 ttcaacagtt aaggaattga ataccttagg aatacataat agtgggtata gaatgttaga
     1381 gaaaagaaaa tgtgctgact atattcctaa tgaaaccatt acgaaaacag atatggcgct
     1441 gatgaatcga gaagcagaaa aaataataaa caaactcacc taatgaattt caagcgagtt
     1501 tgtttgttat ttttttattt aatctttgta
//
LOCUS       DACDIG010000182         1508 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.188, whole genome shotgun sequence.
ACCESSION   DACDIG010000182 DACDIG010000000
VERSION     DACDIG010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.188"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..737
                     /locus_tag="IV247_002925"
     CDS             <1..737
                     /locus_tag="IV247_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAP8778913.1"
                     /translation="SKTIEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLEN
                     MVFNLSNGIIPSVSGDTIRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENE
                     LAVSLPEVLKVRDSGIVYYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANY
                     FSVSETKLRESFKNEMHITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFS
                     RVFKKYAGVSPKQYQLGLVDNFNNIS"
     gene            754..1188
                     /locus_tag="IV247_002926"
     CDS             754..1188
                     /locus_tag="IV247_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778914.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
BASE COUNT          563 a          173 c          261 g          511 t
ORIGIN      
        1 taagtaaaac tatcgagcaa atcaaaatag ataatctgtt atcacaaaat tacgatccag
       61 aaatatatct gtttctttat gaaaataaaa tactggaata tgttaaaaat ggagatattc
      121 gaaatttaga aaacatggtt tttaatctga gtaatggaat aattccaagt gttagtggag
      181 atacgattcg gtctgaaaaa aattattcta tcattgtttt tgaaaagtta gcacaaacgt
      241 ctataacatt aggaatggat ataatagaag cgtatcaaag tagagatgct ttgatacaag
      301 aaaacgaatt agctgtatcg ttacctgagg tcttaaaagt gagggattct gggattgttt
      361 attatactaa ggaaatcgga aaaactaaga tagagcatct ttctccctta atttcttctg
      421 tcgttcaatt catcggctta aatatctata aaagaattac tgtaaaggaa atagctaatt
      481 atttttctgt aagtgaaact aaattaagag aaagctttaa gaatgaaatg catattacga
      541 tttataatta tatctctaag cgaaaaattt ctacggctaa aataatgttg aaatcaaacc
      601 atactatcag tgaagtttct ttaggtttag gattttcaga ttcttcacac ttttcgagag
      661 tattcaaaaa gtacgcaggt gtatctccta agcaatatca attaggttta gtggacaatt
      721 tcaacaacat tagctgagag gttttatttt attatgagac tatatcattt acgtagtttg
      781 gaggagttga ggaaattgga ttttaacaaa aaattttttt gggaaatttc tgatttgagt
      841 tgtttcgtag aatctgagcg tctaagtaat ggtaataata gtgtgttaaa tgaatatgac
      901 aaagtagtaa ttaataaagt aaaaagttta tgtgaagaag ctagagaatc tatcagtcat
      961 attaatggta gcaattttaa acaaacatta tttaatctga ttaagctaga tgctaaaata
     1021 tcaagttatt tattcttttt gatacatgat gaatttatgg attatcaaaa attggatcag
     1081 ttaattgaaa gagaaagctg ggaagattat tacgtgggtc taactttttc agaaaagtta
     1141 aacaactaca agttaataga ttataagtat ttgagtgaaa gcaattaatt attcatgtag
     1201 agtactagac actttaaata tggacaattc aattataaag aaaaagaatc aataatttaa
     1261 tgaggctgtg acaaatgtca ttttttgttt cgctaaaaac gagtgaaaaa atgttgataa
     1321 ctccaatcat tcgtttaaga ttctaggaat cattcagaaa tgctgtgaaa ccgatacttt
     1381 ataattataa agaaggtgat ttcaaattcc gaagaaggaa tttgaagtca cctttttact
     1441 tatgtcacag tctcattcag taatgcggga taaggctcct tattccgtta tttgaatact
     1501 ctgaagtt
//
LOCUS       DACDIG010000183         1500 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.189, whole genome shotgun sequence.
ACCESSION   DACDIG010000183 DACDIG010000000
VERSION     DACDIG010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.189"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1457
                     /locus_tag="IV247_002927"
     CDS             <1..1457
                     /locus_tag="IV247_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAP8778915.1"
                     /translation="QDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDTVANG
                     GLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIVLALP
                     FGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGALLAP
                     LIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEERKGFI
                     RNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVTAEEK
                     PRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNPIFIF
                     ILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPLWLLG
                     SFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFYTPGT
                     ESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          408 a          288 c          317 g          487 t
ORIGIN      
        1 agcaagacaa gggcttcttc gggcagccga agggattgtc aactttgttc ttcactgaga
       61 tgtgggaacg atttagttat tacggtatgc gtgcattact tatctattat atgtacgata
      121 cagttgccaa cgggggactt ggattaccac gagcaacagc attagctatc atgtcgatct
      181 acggatcttt gatctacatg tctagtatcg tcggcggatg ggtatctgac cgagtgttag
      241 gagcaaaacg cactgtattc ttcggtggag ttttcattat gcttggccac atcgtcttgg
      301 ctttaccatt tggtgtaagt acattgtttg tttcgatggc attgattatt atcggaaccg
      361 cctttttgaa accaaatgtt tctggcatgg tcggacatct ttattcaaag actgacttgc
      421 gaagagatgc aggattttcg attttttaca tgggtgtcaa cttaggggca ttattagcac
      481 cactaatcgt tggtacaatc ggtcaagaat ataactatca tttagggttc tctttagctg
      541 ctattgggat gttctttgga ttattacaat acatcatcca aggacgcaag acattagacg
      601 gagtgggtac aacaccgccg aatcctttga caactgaaga acgtaaagga ttcatccgta
      661 acttgatcat cgcacttgtt gttatcgctg cagtattcgg cggggcacaa ctaactggtc
      721 atctgacaat tgactttttt gtcaatacaa tcagtgtatt ggggattatc ttaccgttgt
      781 attactttat caaaatgctg acttcaaaag atgtaactgc tgaagaaaaa ccaagagtat
      841 gggcttatat cccattattc cttgcagcaa tcattttctg gtcattacaa gaacaaggtt
      901 catctatctt agctttattt gctagtgaac gtacacaatc tacgctattt ggtgtaacga
      961 tccaagcaag ctggtatcaa tcattgaatc caattttcat ctttattctg acaccagtat
     1021 tcgtaacgat gtggacgaaa cttggtgaac gtcaaccatc tacagtagtg aaattctctc
     1081 ttggactttt atttgcagga ttatcgtact tgctattgat gttgccgggt atgctttatg
     1141 gtacaagtgg aaaagtaagt ccattgtggt tgttgggaag tttcttcatc gtggttattg
     1201 ctgaattgtg tctttcacca gttggtttgt ctgtgacaac aaaattagca ccacgagcat
     1261 ttgaatcaca aacaatcgct atttggttct tagctgatgc ttcttcacaa gcaatcaatg
     1321 cacaaattgc acgcttctac acaccgggaa cagaatctgc ttacttcggt attatcggtg
     1381 cagtagcaat cgtcggtggt atcttgctat ttactgtgaa agaaccaatc aaaaaactaa
     1441 tgggctctat tcattaatca atgaacataa aaacaggaat tgcttatttg cagcagttcc
//
LOCUS       DACDIG010000184         1494 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.190, whole genome shotgun sequence.
ACCESSION   DACDIG010000184 DACDIG010000000
VERSION     DACDIG010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.190"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            187..699
                     /locus_tag="IV247_002928"
     CDS             187..699
                     /locus_tag="IV247_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778916.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
     gene            970..>1494
                     /locus_tag="IV247_002929"
     CDS             970..>1494
                     /locus_tag="IV247_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8778917.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIV"
BASE COUNT          582 a          182 c          247 g          483 t
ORIGIN      
        1 aaattaaata ggtttcaaga aatagttgaa gatagtgatt ttaacacttt taatagccat
       61 aaaccgatga gaggtactta tataaaattt ctcgaaaatt ataatgattt aaaatctacc
      121 aataattatc aatttggaaa cttgtcttat gagatgataa gtaaagttct aagtttgtga
      181 tgataaatga aattaaatag tataaatgat ttatttgcta ttataggtgt tattgctaac
      241 atactttttt cgtgtttgaa ttttagattt actcaaaaga acaaaaagaa caaactcagt
      301 atagaacaaa tgaaaaaaga cattgaaact gatttaaaaa atgaccataa agaaagaaga
      361 aaaattgaag aatatacttc tttaatggaa aagtgtaaag caactgatgc aatttttagc
      421 tttttaagaa aaagtgatga gataatcaca cgtgaaaata tagatttaga aaatgagatt
      481 agtaaacttg aatctatctt ttccgatata aattctctta agtatttaat cgtcgaagaa
      541 aattcggata gctttttaga atttgaaaaa tatctaacag atcttataga taatagaaat
      601 aatgaattaa tgagacaaaa cattgcaaga ttccttacta aattagtttc tgaatggaaa
      661 agtattattt ctcaaaatat ggataaatta agatattaag gatgttagtt ttaaaataaa
      721 ctaatgtcct tatttttgtg ttcacatttc tgttacctaa gaaatactta aaggaggtga
      781 tgtagccagc aacgactaca acttgaaccc gctatctaca gtttatttgt tgtttctttt
      841 gggtatactc taatggtaca aatccatatt tgcttaaagc gttgattttt cagcggtttt
      901 tctttattgc aaagtgtacc ctaagacctg tatactaata gtaaatagat tggataggaa
      961 tgatggcaaa tgtatttagc ttatttaaga gtttcttccg acgatcaaag tttagctcgg
     1021 caacataagc taattgaaaa ttggtcagtc aaaaataata ttgctgaaga cgatttaaaa
     1081 attttcgagg aaaaagtctc tggaaaaaat attgaaaaca gaccagagct gcaagagctt
     1141 atttctttca tacgtgaacg agatacgatt attattccta gtcttgatcg attagcaaga
     1201 aatagtaaag atacaaaaga cttacttcaa ttgtttcgta gtaaaggtgc ttctattgaa
     1261 atattagacc taccgtcgtt taatggggta actgaccctg cattaagaga cttgctaaca
     1321 aatttagtca tagaagtgtt tagctatgtt gctgaaaacg aacggaaaaa aatcaaagag
     1381 cgtcaaaaac aaggcattga aattgcaaag aaaaatggtc gttatactgg taaaaagatt
     1441 caataccatg ccaatgcaac aggaaaaaac aaattgattt acgatgaaat tgtt
//
LOCUS       DACDIG010000185         1458 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.191, whole genome shotgun sequence.
ACCESSION   DACDIG010000185 DACDIG010000000
VERSION     DACDIG010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.191"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..321)
                     /locus_tag="IV247_002930"
     CDS             complement(<1..321)
                     /locus_tag="IV247_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778918.1"
                     /translation="MKEISANEAHIPQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKATSIRELRSEVEKIRLGLTSEEIAKLKGNNSFVIEKEKEKHLLYRELKIEEYYEI
                     DLPEK"
     gene            complement(452..1174)
                     /locus_tag="IV247_002931"
     CDS             complement(452..1174)
                     /locus_tag="IV247_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778919.1"
                     /translation="MKSTKLKVVLLSTAALITLTACGTTNSANNNKDTKSSSTMVTKN
                     STTSASTTMSSMTENNAEKYQKYEDINKGQKPDEVYNNFINTVKNDENYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIKEGFSYAPTLMHDITINKPAYEYYAVAVDITDGV
                     PEWVQNIPGLADENNQYHTPTVAVVTPKKVNGTLYWDVMGMTTNTTDEMIESTTKYYM
                     DLGAYKLYQQSEPVMDDQSKVK"
     gene            complement(1180..1425)
                     /locus_tag="IV247_002932"
     CDS             complement(1180..1425)
                     /locus_tag="IV247_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778920.1"
                     /translation="MKKIINQPTNHLIIEGVIFVTMYFTVGNGISNTIIQKNPQYLLF
                     SLYAWIVQMVWVSILMLLFYWIVRVILLLLNKRKKDR"
BASE COUNT          409 a          258 c          200 g          591 t
ORIGIN      
        1 tttttcaggt aaatcaattt catagtattc ttcaattttc agctcacgat aaagtagatg
       61 tttttccttt tctttttcaa ttacaaaaga attatttccc ttcagtttag ctatttcttc
      121 acttgtgaga ccaagcctaa ttttttctac ttcacttcta agttcacgaa tacttgttgc
      181 ctttaaccac ttcccatttg caccattatt gtaaccggta agtaactgta ttttataaat
      241 tccttcacca tgaagcacct tatatatacc aacctgattc ttttgcggaa tatgagcttc
      301 atttgcactg atttctttca ttacgcattc tcctttataa tctagttaat tagcttgtgt
      361 gtttcgagta gttgagtaag aacgtttgtt tcagaaatca tttatttgtt atcacttaat
      421 tttaaaaaaa tagggcttac gccctaaaga tctatttaac tttggattga tcgtccataa
      481 caggctctga ttgttgatat aatttatatg ctcccaaatc catataatat ttagttgtac
      541 tttcaatcat ttcatcagtt gtatttgttg tcattcccat tacatcccag tatagtgtgc
      601 cgttaacttt ctttggtgtt actactgcaa ctgttggcgt atgatattga ttgttctcat
      661 cagccagacc aggaatgttt tgtacccact caggtacacc atcagtaata tctacagcaa
      721 ctgcgtaata ctcgtaagct ggtttattta tggtaatatc atgcattaat gttggagcat
      781 aactaaatcc ttcttttatt tcttgcatga tttttgattc tgctttttga caatctggac
      841 aatcaggttt gtaaagaacg atagtcagat ttttattttg caatggatca ttcccataat
      901 tttcatcatt ttttactgta ttaataaagt tgttataaac ttcatccggc ttttgacctt
      961 tattaatgtc ttcgtatttt tgatactttt cagcattatt ttcagtcatt gaagacattg
     1021 ttgttgatgc cgatgtagtg ctgtttttag taaccatcgt actactagac tttgtgtctt
     1081 tgttattatt agcactatta gttgtaccac atgctgtaag ggttattaat gctgctgtcg
     1141 ataacaatac aaccttcaat ttagttgatt tcattttatt cacctatcct tttttctttt
     1201 gtttaacaat aaaagaataa cccgtactat ccaataaaac aacagcatca atatggaaac
     1261 ccaaaccatt tgtacaatcc aagcataaag actaaataat aggtattgtg gatttttttg
     1321 aatgattgta tttgagatgc catttcctac agtaaaatac attgttacga aaataacacc
     1381 ttcaattatc aagtgatttg ttggttgatt tataattttt ttcatatttt tactcctaaa
     1441 ctaaatttat tcttcctg
//
LOCUS       DACDIG010000186         1410 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.193, whole genome shotgun sequence.
ACCESSION   DACDIG010000186 DACDIG010000000
VERSION     DACDIG010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1410
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.193"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            73..528
                     /locus_tag="IV247_002933"
     CDS             73..528
                     /locus_tag="IV247_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778921.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            647..>1410
                     /locus_tag="IV247_002934"
     CDS             647..>1410
                     /locus_tag="IV247_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HAP8778922.1"
                     /translation="MKKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLDERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVA"
BASE COUNT          541 a          199 c          268 g          402 t
ORIGIN      
        1 ttattatgat attataataa cataaacgtg ttacgcccga acctcatttt aaaaaaatga
       61 aggggtgaga caatgaatgc tattaattgg catgaatttg ttaactatat ggagagctta
      121 ctagatgatc taaaaagttt tagtattgct gatgcagtaa agcatgggtt accagttagt
      181 acaacgatga aattacgagg attgaaaatt ccagatgaaa ttattaagct gagaactaaa
      241 acaattattg aaaccgcaaa agtcgttgac aatatgaacc atgaactaaa aggcaagaaa
      301 acaacttacg agaaagattt tgaacgtttt ttaaaatctg ttattggaaa atatctaggt
      361 aaaaaatctt tcagtgaatt gagagatgaa gagattgtac ctactccaat ggttagcaaa
      421 atccgaaaaa acaaagccga ggggaattac tatcttggta caatagcaaa aatcattgat
      481 tatttggaaa ataaagagca aaagaaagga acgaagaaag gaagataatt tttgtgttaa
      541 atattaatgg ggaagaatat accgaaatga ttcaaaatcc cgaacctaga attaccgaaa
      601 aaacgtctga ttgtttgctc ttttatgaaa gggggcagac aaactaatga aaaaaaacgt
      661 caattcaaca tctaccaaag atcaatacag tattattgga gggttgcttg gtgataagga
      721 taagagaaca cgtcacggat tacgcattat gcttgcaact tttctaggag tgcttttttt
      781 attaacaggc ttgggtagct acatttatca attaggaaac tataattatt atctcgtgaa
      841 caatacaaca cctataggct cttcattgga gtttgctcga tcaggagcag agatcactat
      901 ttcagatgtg tggacagatc aaaacagaga tgtaacagta gtaaaactcg gttactcgca
      961 aaacgctaga aagttgttat ctacgcaagg aaaaaactac aacttatata tggcaacgaa
     1021 agaaaaagat cgtcctaaaa agcttgaaat gagctatggg atgttaagta ctgatgggga
     1081 tgctttctta ttcctaaaag gtaaattaga tgaaagagct tatcaagttt ttattgcaaa
     1141 ccaagtatca ctaatttctg gtgatgatac agctgacgat gataccaaag tcaatgtgaa
     1201 tgaagcagat tcagttacaa aagcactttc tcaatattct atgaatgatg ttaataatga
     1261 aggagtaatt tcttttggga aaaaagcaaa acctgttaat actcctgata atatcaattt
     1321 cagaataaat ccatattcag attcaactaa tgtctatcaa ggaagctttc ttaccaaaga
     1381 tggcgatatt gattattcaa aagttgtagc
//
LOCUS       DACDIG010000187         1405 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.194, whole genome shotgun sequence.
ACCESSION   DACDIG010000187 DACDIG010000000
VERSION     DACDIG010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.194"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1090
                     /locus_tag="IV247_002935"
     CDS             <1..1090
                     /locus_tag="IV247_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP8778923.1"
                     /translation="STGTILGWQFYNNEHSQIVAHAQSAQKKLILTTNEQQLVEKIDQ
                     LLRKNDYIGSIYVKKNNRIIMEKGYGYANKLTQQPNDPSLYYQIGSVQKALTTLLILK
                     QVERGHLSLDTKLAQFYPQIAGSQQITIRDLLYMRSGLHRTASPTIPMTDEEVVQFAI
                     QHLKFTDYQTSHYDSLNFTILTGILIKLTNQPYEKLLTDDIIEPLGLQHTKFYDQVKN
                     SSNHALSYQMSKEDDYFKPLTESETEIHNELGTGNISMSVYDLNTLFTQVLSGKLVSK
                     DLLFSLWRENANGHPYSGGVYSGDNYILSQGNIDSFDTSAIFSKDSQDAIIMESNIKA
                     DKTIKLPTADLRNQIYDLMEGTTKLANS"
BASE COUNT          499 a          259 c          225 g          422 t
ORIGIN      
        1 ctctacagga actattcttg gatggcaatt ttacaataat gaacactctc agatagttgc
       61 ccatgctcaa tcagctcaaa aaaagttgat actgactacc aatgaacaac agctcgttga
      121 aaaaatcgat caactattaa gaaaaaatga ttatattggc tcaatttacg taaagaaaaa
      181 taatcgcatc atcatggaaa aaggctatgg ctatgcgaat aaactcacgc aacaaccaaa
      241 tgatccttct ctttattatc agattggttc ggttcaaaaa gcattgacta ctcttttgat
      301 tttaaaacaa gtcgagcgtg ggcacctttc tttagatact aagctggctc aattctatcc
      361 acaaattgct ggcagccagc aaattactat ccgtgacttg ctgtatatgc gctcaggctt
      421 gcaccgaact gcttctccaa ccataccgat gactgatgaa gaagtcgttc aatttgcgat
      481 tcagcactta aaatttacag attatcagac ctctcattat gattcattga actttaccat
      541 attgactgga attttgatca aattaacaaa tcaaccgtat gaaaaattat tgaccgatga
      601 tatcatcgaa cctttaggat tacagcatac aaaattttat gaccaagtca aaaattcatc
      661 caatcatgca ctatcctatc aaatgtctaa agaggatgat tactttaaac cactgacaga
      721 atctgaaaca gagatccata acgaattagg gactggtaat atcagtatgt ccgtctatga
      781 tttaaatacg ctgtttacac aagttttatc tggaaaacta gtttccaaag acttactctt
      841 ctcattatgg cgtgaaaatg cgaatggaca tccatatagt ggtggggtat atagcggaga
      901 taattatatc ctgtcgcaag gaaatatcga ctcttttgat acatctgcta tatttagcaa
      961 agattcacag gatgctatca tcatggaaag caatataaaa gctgacaaaa ctatcaaact
     1021 gcctactgct gatctaagaa accaaatcta tgacctgatg gaaggaacta ctaagttggc
     1081 aaatagctga tatgatgaaa agatagcaaa tactacttct ttgaatagca tttgcttaaa
     1141 aaaagtactc gactaaacgt aggaaagagt gtgggacaaa tctaaaaatc aatttttgct
     1201 acagacttta attccaaata aacagaacag aaagaggtgt tcttctttct gttctgtcca
     1261 aattattcta agatttctct ttaccaacat tatttgacaa agggcctatt aaatctaaaa
     1321 tagtatcttt agattgctaa aatatgtgct agcaaatcat tcatggcttg ttcttatttt
     1381 taattttatg caataaaaaa gaaga
//
LOCUS       DACDIG010000188         1368 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.195, whole genome shotgun sequence.
ACCESSION   DACDIG010000188 DACDIG010000000
VERSION     DACDIG010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.195"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            82..>1368
                     /locus_tag="IV247_002936"
     CDS             82..>1368
                     /locus_tag="IV247_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAP8778924.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLIN"
BASE COUNT          467 a          263 c          257 g          381 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taatcaat
//
LOCUS       DACDIG010000189         1320 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.197, whole genome shotgun sequence.
ACCESSION   DACDIG010000189 DACDIG010000000
VERSION     DACDIG010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.197"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            298..1101
                     /locus_tag="IV247_002937"
     CDS             298..1101
                     /locus_tag="IV247_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP8778925.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKDLSDKLGNLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
BASE COUNT          503 a          165 c          260 g          392 t
ORIGIN      
        1 aaaggtaaca cttttggaca gtaaaggtaa caaaagtgga cagtaagggt aacagttttg
       61 gacagcaaag gtaacaaaag tggacatttg aggtatcaca ctaggataag acgttacctt
      121 cggttttctt gatttggaag gcgaaaaaat agaaaattag attagtaaca aatctttgtt
      181 gactgttacc tttagttgtg atttaattta ggtaacaagc agaaaaaatt gtttccttta
      241 attagtacca gattaacatg attaaaggag cgcaaaaaga agattggaga gtaatcaatg
      301 tctcaagaag ttaccgaaaa gattattgat agtgatgtag atttagtatc agaacaaaca
      361 aatgcaattg tgcgttataa caatggtctg aatacaacag ctttgcgagg atttaagcct
      421 gttgaaatgg atgtgttttg ggctatttta tcaaaaatga aacgtcaagg aacagcagaa
      481 gtaacttttg atttcaacct ctttcgttat ttggtcaaat acgacagaag agaaaaggac
      541 agtttttatt ctgtgttaaa agatttgagt gacaaactag gtaatttaac ctaccgtttt
      601 gaagattcag acgtctatga acagctgtgg ctttttcaaa aattccggat agataaacag
      661 aaggaaacag ttacaattaa ggcaaatgag gaatttgaat ttatcttaaa ctcaattggc
      721 aaaaacttta ctcgttttga acttgataat tttacaaaac taagaagtag ctacacaaaa
      781 gaattgtatc gtcatctcat gcaatacaga aataaagagt ggcgaaatgg ttattgggta
      841 gtcaaagttg aagattttag aagagcatta agtatacctt caagttatag aatgacaaat
      901 attgataaaa gaatactaga gcaggcgaaa gaagaatttt tagctcctga tgaaaacgga
      961 atacaggtat ttgaaacttt tgattatgaa aaaatctatg caaagaaagg aagaagagtt
     1021 gatcggctca gattcacttt tagtgagcca gaatcaaact tgccaaccat ttcaatgcat
     1081 aattggttag aagaagattg agaagaagca ggttgtaatt ttaatacaac ctgcttcttt
     1141 aaaaaagtat gggtaacact tttggacaat aaatgcaaga agtttttttg taagcaagtt
     1201 tcagtctaaa taatatttga aagaatggct aatctataca aattttaaca actatgggta
     1261 acacttttgg acaagtcaag aaaaaaaaca atttttctta atttgttgat cgactaaagg
//
LOCUS       DACDIG010000190         1294 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.198, whole genome shotgun sequence.
ACCESSION   DACDIG010000190 DACDIG010000000
VERSION     DACDIG010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.198"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1244
                     /locus_tag="IV247_002938"
     CDS             <1..1244
                     /locus_tag="IV247_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP8778926.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DACDIG010000191         1292 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.199, whole genome shotgun sequence.
ACCESSION   DACDIG010000191 DACDIG010000000
VERSION     DACDIG010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.199"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            37..258
                     /locus_tag="IV247_002939"
     CDS             37..258
                     /locus_tag="IV247_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAP8778927.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKQLMKEHEENDQSISHEDMLKELGL"
     gene            260..526
                     /locus_tag="IV247_002940"
     CDS             260..526
                     /locus_tag="IV247_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP8778928.1"
                     /translation="MTYQVRYEREAQKALKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            639..1178
                     /locus_tag="IV247_002941"
     CDS             639..1178
                     /locus_tag="IV247_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778929.1"
                     /translation="MYNISVNKLINFQKERFFMGWYGDYSSANEVIEEKKGYAKNGLE
                     IVGTKVTKTYGIILFKDAEGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFF
                     VGDQEKMVKLQLDRIKEESNKPKLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQ
                     DGSLTRFTRLSAGDVKEVT"
BASE COUNT          478 a          198 c          247 g          369 t
ORIGIN      
        1 cgtagtacaa tgtaattgaa aaggagttga taaactatgc cagtaataag tattcgtgta
       61 tctaaagaag aaaaagattt tcttgaaaaa gttgctgaat tcaacggaga taacatttct
      121 gaatttatgc gtagtaattc cctgcaaaca gcagaaacgt tagtagactt tgccacctac
      181 aagcaactaa tgaaagagca tgaggaaaac gatcaaagta tttcccacga agatatgcta
      241 aaagagttag gcttataaga tgacttacca agtaagatat gaacgtgaag ctcaaaaagc
      301 gctaaaaaaa atggataagt ttcaggcaaa ggttatccta aactggatcg agaaaaatct
      361 tgtaggcact gatgatccaa gaaagcatgg taaaggtcta acagctaata aatcaggcta
      421 ctggcgatat agggtaggtg cataccgtat aatagctgat attagcgagc atgaagtgac
      481 aattcttata ttgtctattg gtcacagaag tgaagtttat aagtaacaga caagctgata
      541 aggcttgtct gtttttttgt taacccaaca aaatcactgt ttacgatccc actttaccta
      601 aaaaagaaca atatcctctt gatattatac acaccagtgt gtataatata agtgtaaaca
      661 agctaattaa ctttcaaaag gagcgatttt ttatgggatg gtatggtgat tattcatcag
      721 caaatgaagt gatcgaagaa aagaaagggt acgccaaaaa tggtttggaa atcgtaggta
      781 ccaaagtaac gaagacctat ggaattatcc ttttcaaaga tgcagaagga acctacgata
      841 ttgacagctt catctttcga gatgaaatgt acaaaccaat ctacttctta aatgacagtc
      901 ctgcttactt taagaaaatt ccgaaaaaat ggttgaaatt ctttgtcggt gatcaagaaa
      961 aaatggttaa gctacaacta gatagaatta aagaagaaag caataaacca aaacttgatg
     1021 acttattagt tgcaggtaaa acttactcaa tctgggatgg atcgtatgtc gctgtctatc
     1081 agtataaact caaacgtagc tttatttttc gactacaaga tggatcactc actcggttca
     1141 ctcgactatc agcaggagat gttaaggaag taacctaaaa cttaaataca taaacataaa
     1201 cgtgtatacg tttatgttta tgtatatgta tatgaatata aacgtattag gtacgaacac
     1261 ttttaaattc ttttctgggc tttgttttta gg
//
LOCUS       DACDIG010000192         1231 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.200, whole genome shotgun sequence.
ACCESSION   DACDIG010000192 DACDIG010000000
VERSION     DACDIG010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.200"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>1231
                     /locus_tag="IV247_002942"
     rRNA            <1..>1231
                     /locus_tag="IV247_002942"
                     /product="23S ribosomal RNA"
BASE COUNT          323 a          280 c          375 g          253 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
      901 atctccccca agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
      961 tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag
     1021 ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt tggaaatttg
     1081 agaggagctg tccttagtac gagaggaccg ggatggactt accgctggtg taccagttgt
     1141 tctgccaagg gcattgctgg gtagctatgt agggaaggga taaacgctga aagcatctaa
     1201 gtgtgaagcc cacctcaaga tgagatttcc c
//
LOCUS       DACDIG010000193         1228 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.201, whole genome shotgun sequence.
ACCESSION   DACDIG010000193 DACDIG010000000
VERSION     DACDIG010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.201"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(20..361)
                     /locus_tag="IV247_002943"
     CDS             complement(20..361)
                     /locus_tag="IV247_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP8778930.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     KKSDQIKRVYFPIGEWEKAGLKKQSWIDVGSLKSIPKSRENISFKKVGMLSASDTKKL
                     NKFIERLQRKV"
     gene            complement(351..632)
                     /locus_tag="IV247_002944"
     CDS             complement(351..632)
                     /locus_tag="IV247_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAP8778931.1"
                     /translation="MKVKNPTEKSRIQVGIDKNLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTKDMPVDVLDTDEKLEEWFDEA"
     gene            complement(1003..>1228)
                     /locus_tag="IV247_002945"
     CDS             complement(1003..>1228)
                     /locus_tag="IV247_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778932.1"
                     /translation="SKGMQWCSYYYVGNDEDLLRKTQRKGYPDKILKQIGYYMPEERI
                     EKEFCEVLFDSEQLLVLLTQNKLICQFENN"
BASE COUNT          359 a          260 c          140 g          469 t
ORIGIN      
        1 caaaaaaaga aaagccttgt tagacttttc tttgtaatct ctcgataaac ttatttagct
       61 ttttcgtatc actagctgat agcataccaa cctttttaaa tgatatgttc tctcggcttt
      121 tgggaataga ctttaaagac cctacgtcaa tccatgattg ctttttaaga cctgcttttt
      181 cccattctcc aataggaaaa tacactctct ttatttggtc tgatttcttc tcatattgac
      241 ttgtgattcc aaaaaattct actctatctt ctttatcact aaccaccaaa ataggtcttc
      301 ttttaccacc atttttatct gtaaatgcaa catagaccgt tactatctca ttaggcttca
      361 tcgaaccact cttctaactt ttcatctgta tctagaacat ctactggcat gtccttagtt
      421 agctgttgca atctgattcc ttgcttctcc tcttcactta gctttatctc tactggaaac
      481 tcacctctag cgactacttg tttgtaaaga attgttattg ctgttgtagg atttaaacct
      541 agctcttcca atatcatttc tgcgttctct tttaaattct tgtctattcc gacttggatt
      601 ctgctttttt cagttggatt tttgaccttc atttcatcca ctccttttga gtataaaaca
      661 ctcattattt ctcaaattat ccacatttat gattatatta tactcttaat aaactacatt
      721 gcaagtaatc taatcaaatt aagatgctat atcatacggt aattgaacaa attgtcgcta
      781 acccacacat acggaatcgt aatgaatgaa aaaaagacaa gcctattcgc agtaggcttg
      841 tctttgtgct tctcttgtac aaaatcttca catttgtaaa ttaattttag cacctttgta
      901 aggaaagaac aacttaaatg agatgctaaa gaattaatag ttctcaatct agcatattca
      961 cttaatacca tcttatcttc attcatggtt caccacctca tcttaattat tttcaaattg
     1021 gcaaatcaat ttattttggg ttaacaaaac taatagctgt tcactatcaa aaagaacctc
     1081 acaaaattct ttttctatcc tctcttctgg catgtaataa cctatttgtt tcaatatctt
     1141 atctggataa ccttttcgtt gggtttttct caataaatct tcatcatttc caacataata
     1201 ataagaacac cattgcatac ccttactt
//
LOCUS       DACDIG010000194         1207 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.202, whole genome shotgun sequence.
ACCESSION   DACDIG010000194 DACDIG010000000
VERSION     DACDIG010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.202"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(79..375)
                     /locus_tag="IV247_002946"
     CDS             complement(79..375)
                     /locus_tag="IV247_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778933.1"
                     /translation="MIEVKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAK
                     LKSDSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(379..1197)
                     /locus_tag="IV247_002947"
     CDS             complement(379..1197)
                     /locus_tag="IV247_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8778934.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAINENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWIVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
BASE COUNT          348 a          245 c          166 g          448 t
ORIGIN      
        1 ataaacacgt atacgaaatt tagttaagct atacctaacc gcttattcca ataaaaaaag
       61 tgatcagctt taaactgatc actcaccaaa aacttcatca tacaagttat ctatcatttc
      121 accaagtaaa gctgaatctg atcgaattcc taacttttcg ctgattttag atagtttctt
      181 tctttgtgat ggttgaagcg tgaattgata atttttcttc ttttcagaac tatcactttt
      241 tagtttagct accaaagaac ttgttgtttc tttaggtgtt tgtttaggtt cttcaactgc
      301 ttcaaccact tcaggaactt cttctacttc agcttcagcg aattttttaa atctattttc
      361 tttcacttca atcactcctt attcttgtgc cgtatcaatt attttgttgt atagcgtaaa
      421 cgtgctttca aagaaatcct tattcttacg atagatattc ttatccttag cgaactcagc
      481 aatattctta ccttctaagc tagttgcatt aagcaattcc ttctcttgta catatccaag
      541 ataattagat aacgttggta ccaattctag gaactcacga cttgaattag tattatgttt
      601 gatcctgcta ccgatcgtag cgtatttagt ttccatcagt gtttcaccag tcttaatatc
      661 aatcaactgt tttgctagtg catcaatatc tttaccaagc ttaagcaaag cttcaaaacc
      721 atctttagat ggatcagaaa tgcctacgac aagatcagat acagcccaag cattttgagc
      781 tagtagacct gcatcattat gagtatcaat cagaattaga tcatacttct ctttaagctg
      841 ttcatagtta acaacgatcc atctaaacag tagggttctg ttgttaggca tatcacgcat
      901 ttgcatctca acttgtttta aatcttcacc tgctactaaa atatccatgt tctcatttat
      961 agctaaaggt tcagctgatc catgaagata taaatgattg attgtattct ctcttttcgc
     1021 tttactgaaa gcatcaggaa atcgttttga taggttttgc gacttatctt catcaatata
     1081 cagcaccttc atacctaaat tttcgacagc atatccacct agattgtaga ttgttgttgt
     1141 tttacctgtt ccaccttttt caatgtttcc agtaatgatt gacaacttct tttccatgat
     1201 taaacct
//
LOCUS       DACDIG010000195         1199 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.203, whole genome shotgun sequence.
ACCESSION   DACDIG010000195 DACDIG010000000
VERSION     DACDIG010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.203"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..454
                     /locus_tag="IV247_002948"
     CDS             <1..454
                     /locus_tag="IV247_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778935.1"
                     /translation="GRYMLMDVLNQGIYLINKAYDLRDKSREELAKGIFDRSTGVINF
                     ATYESGMSAYDETGFDGRYEPYQETIEDVMKFSDNNDGYAHLEMVFDENGYSLDDKTR
                     LRLFDIEGKQIGLNKFCKNGKEFATKDFIKGYRLLLENYGINLEESVK"
     gene            455..1021
                     /locus_tag="IV247_002949"
     CDS             455..1021
                     /locus_tag="IV247_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778936.1"
                     /translation="MFQKRKDKFPNLEVKSQKVIDGEMIELFQKNYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTINGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMEKENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGADDCVAYYTTAE"
BASE COUNT          441 a          132 c          288 g          338 t
ORIGIN      
        1 tggtcgatat atgctgatgg acgtattgaa tcaaggaatt tatttaatca ataaagcata
       61 cgacttacga gataaatcaa gagaagaatt agcaaaagga atctttgata gaagtacagg
      121 ggttatcaat tttgccacct atgaatcagg tatgagtgct tatgatgaaa caggatttga
      181 tgggcgctat gaaccttatc aagaaacgat tgaagatgtg atgaagtttt ctgataacaa
      241 tgatggctat gctcatttag agatggtgtt cgatgaaaat ggatatagtc tagatgataa
      301 aaccagattg cgattgtttg atattgaggg taaacagata ggactaaaca agttttgtaa
      361 gaatggaaaa gagtttgcta caaaagattt tattaaggga tacagattgt tgctagaaaa
      421 ttatggaata aacctagaag agagcgtgaa ataaatgttt caaaaaagaa aagacaaatt
      481 ccctaattta gaagtgaaaa gtcaaaaagt gattgatggt gaaatgattg aactattcca
      541 aaaaaattat ggaacgatag aaactaccgc aagttgcaaa tactactttc aaggaaagag
      601 aaacacgttt aaaggctttg ccgattctga tgaagaattg gcaaagaaaa tcattgattg
      661 gcttgtcagc agtgaacacg tagcaagaga agataccgtt agaacaacga ttaatgggga
      721 agtcctaaca ggtactagat gtgtttgttg taatcatgaa tttgtagaag atgaaggttg
      781 ctaccgaatc aatgaatatg gtgattttat ttgccagagt tgccacgaga tagaatttcc
      841 aaccgaaaga agttggtttg aatacatgct acaagattat gagatggaaa aagaaaatgg
      901 tgaagtagtt ttttgtacca aagaggaatt gagtaagaga agcgatgatg aaattgaaga
      961 ttggtgtgtg tggttagtag gggcagatga ttgcgttgca tattacacaa cagcagaata
     1021 ggagtgtgaa tcagcatgga aggaatggat tttctagatc atgaagattt ggttgatttt
     1081 ggttatacat ggaaaggaat ggtaggtatt agcagaagtc ttgctaatgc cttttacgaa
     1141 agaaattatg cagtatatgt tctttatgat gacgatacag aaaaccttgt tgacgagga
//
LOCUS       DACDIG010000196         1191 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.204, whole genome shotgun sequence.
ACCESSION   DACDIG010000196 DACDIG010000000
VERSION     DACDIG010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.204"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..521
                     /locus_tag="IV247_002950"
     CDS             <1..521
                     /locus_tag="IV247_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8778937.1"
                     /translation="LKEINRDSEIFSLSKDDKIVMGKRIREVRKNREETLEKFGKNFT
                     IYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDYKNILPKDERVSDFKK
                     INSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENGRDIPNTNRIIQYAYI
                     GNLSLEFLIYGI"
BASE COUNT          435 a          135 c          253 g          368 t
ORIGIN      
        1 ttttgaagga gattaatcgt gatagtgaga tatttagttt atcaaaagac gataaaattg
       61 ttatgggaaa aagaatacga gaagtccgaa agaaccggga agagacgctt gaaaaatttg
      121 gtaaaaactt taccatctat acaggcaaaa atgttataag tagatgggaa aaaggtatta
      181 atattccaga tattgaaaaa ctaatgaata ttgcctatct tggtaaggtc actgttcctt
      241 accttatgta cggagaagat tataagaata tacttccaaa agatgaaaga gttagcgatt
      301 ttaaaaagat aaattctttt agtatgggat taaggatgag gaaaattagg aaagattatt
      361 atctagaaag ggaggagttt ggaaagcttt tttctccttc aatttcaaaa tggtccatag
      421 ataggtatga gaatggtaga gatataccca atactaatcg aattatccaa tatgcttata
      481 taggaaatct ttctttagaa tttttgattt acgggatata aaaatagttt acgtcgatac
      541 ttattaagtt atcgacgttt tttattatag aaaaaattta taaaaggagg tttcttgata
      601 tgataagaaa caaaaagggt ttgttgaaag ttgctttact ggcagctttt ttctaagtaa
      661 ctttttgaca agctcaatgg caacacaagc ggaagaagta atacaagaac taactgttat
      721 gaataacagc tagataaaat gggctatgta tgttatccaa caatttagta atcactagaa
      781 gcctagcgaa agttcgcttg ataagttata gatgagtatt gatttgtagt gtttccaaat
      841 caaaagggta cagagccaca tgaaatatgt tgtcctctgc agcgtcagga tgggaatcca
      901 taccatggag attcggagat ctgtgaggga atacttacgt ggttaatatg tcagttgtgt
      961 tggacgtagt aatggtcata gtgggtagat tgactgaatt cttatatgag aaaatgaaac
     1021 caacaagaag atagggttat ttatacagaa ttggggatct tttctggaaa agtaatcgga
     1081 taatgcgaga ttcgtacggt cttttttaaa tttctctaaa aaaaacggca tcgaatgggt
     1141 tgggtgattg ataagcaatg aatcatagac tgatatagaa taaaaaatga t
//
LOCUS       DACDIG010000197         1182 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.205, whole genome shotgun sequence.
ACCESSION   DACDIG010000197 DACDIG010000000
VERSION     DACDIG010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.205"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..113)
                     /locus_tag="IV247_002951"
     CDS             complement(<1..113)
                     /locus_tag="IV247_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8778938.1"
                     /translation="MKKKIFVGAIIALFLLPINAFAYSINNEFNLGANEGSS"
     gene            complement(110..499)
                     /locus_tag="IV247_002952"
     CDS             complement(110..499)
                     /locus_tag="IV247_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAP8778939.1"
                     /translation="MTGVIGGTIVGLLGGMDNILHVLIFLVGVDFLTGLAKAWKLKEI
                     SSEIGFEGLLKKVLIFVVIAVAVDVQKIVGNSIPLREIVIMFYVANEGISFLENISVF
                     LPLPDKLKEVFQQIRNDTENKDRGGTK"
     gene            complement(599..739)
                     /locus_tag="IV247_002953"
     CDS             complement(599..739)
                     /locus_tag="IV247_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP8778940.1"
                     /translation="MNSFPGFENIKQFYDWGCYTDQDLLDYVNMNCLTKDQYKQITGN
                     EI"
     gene            complement(732..1079)
                     /locus_tag="IV247_002954"
     CDS             complement(732..1079)
                     /locus_tag="IV247_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778941.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKSGTAVDDIEDVDQLKQLNALLTKQLAISVQEQEKMQQMLAQLTM
                     EVASIKNGGKSNE"
BASE COUNT          340 a          253 c          161 g          428 t
ORIGIN      
        1 gaactacctt cgtttgcgcc caaattgaat tcattgttga tcgagtaagc aaaggcgttt
       61 attggcaata aaaaaagagc tataatagct cctacaaaga ttttcttttt catttggttc
      121 ctcctcggtc tttattttca gtatcatttc gtatttgttg gaatacctct tttaacttat
      181 ctggcaaagg aaggaatacc gaaatgtttt ctaaaaaact aatcccttca tttgcaacat
      241 agaacataat aactatttct cttagaggta tagaattccc aacgattttc tgtacgtcaa
      301 ccgcaacggc aatcacaacg aagattaaga ctttcttcaa caatccttcg aaacctatct
      361 cactacttat ttctttcaat ttccatgcct tagcaagtcc agttaagaag tccacaccca
      421 caagaaaaat taaaacatgt agtatattgt ccatccctcc caataaacca acaattgtcc
      481 cgcctatcac ccctgtcatt actgagagtg tatttaaata tttttccaat atctatttct
      541 cctttatgca aagaagcgca ctcgtttgag tacgcttttt atttaacttt gtgcttaatt
      601 aaatttcatt tccagtaatc tgcttatatt ggtcttttgt taaacagttc atgtttacat
      661 agtcaagtaa atcttggtcc gtataacatc cccaatcata aaattgtttt atattttcaa
      721 agcccggaaa actattcatt acttttccct ccattcttaa tacttgcgac ttccatcgtc
      781 aattgagcta acatttgttg cattttttct tgttcctgta ccgatatcgc caattgtttt
      841 gtcagtagag catttaattg cttcaactga tcgacatctt caatatcgtc aaccgctgtg
      901 ccactcttct cttcttcagt agctaactca acccactgtt ttttatcaaa atcaaatctt
      961 ggtctccagt taggaactgg tggtttaatc tctgtacagt tttcagggat attttcttga
     1021 ttgttcaaaa tgatttgctc gaatccgtaa ggcttgattg atttgtaaac tactttcatt
     1081 agatttcctc ccttaaattg aataagtgat aacaaatgaa taagctgatc cataacttga
     1141 atttcttctc catttaatgg ctccgtctgc gccaatagat ag
//
LOCUS       DACDIG010000198         1158 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.206, whole genome shotgun sequence.
ACCESSION   DACDIG010000198 DACDIG010000000
VERSION     DACDIG010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.206"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..119
                     /locus_tag="IV247_002955"
     CDS             <1..119
                     /locus_tag="IV247_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAP8778942.1"
                     /translation="LILDIDSEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            124..1071
                     /locus_tag="IV247_002956"
     CDS             124..1071
                     /locus_tag="IV247_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP8778943.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          373 a          171 c          245 g          369 t
ORIGIN      
        1 atctaatttt agatattgat agtgaagaag attttaaatg gttagaattt ttgtttccta
       61 aatttttaga agattcttcg gagcttcaag atatctataa ttatttatat cggaggtaaa
      121 aggatgagct gttatcttat caccggtggg gctggcttca ttggttctac cttagccaat
      181 catttaggag aagaacatgt ggtaatcgtc gtcgatgact tatcaatggg aaaagtagaa
      241 aatcttgata tgacgaaaaa tatcactttt attgagggag atgtcgctga gaaatccttg
      301 atggaagaga tcatgaaagc taataaattt gattatatct ttcatttagc agcagttgct
      361 agtgtggcag attcagttgc ccgaccagta gaaacacatc gtgtgaattt tgaaagtgta
      421 ttaatgattt tagaattgat ccgcaagtat caacctgatt taaaacggat cgtgtttagt
      481 tcttcagcgg ctgtttatgg ggatgaaccg actttaccaa aaaaagaaga gtccgtgatt
      541 cgcccattga cgccttatgc ggttgataaa ttcgctgcag aacaatatgt gctagattat
      601 tgtcatttat atgatgtacc cggtagcgca gttcgttttt tcaatgtcta tggtccaaat
      661 caaaatccga attctccata cagtggtgtg atttctattc tcgtagatcg ctataagaaa
      721 caaatcgtag gagaagcaac ttcatttaca ttatttggtg atggaagcca gtctagagac
      781 tttgtttata tagatgatgt aatccaagca ttacttttag ttgcgaatga ggaagctgca
      841 ctggggaaac aattcaatgt tggaacgggt aaagcaacca cacttttgga attgattcat
      901 gcaatcaatg aaattctcgg tgtagaattg acacttgaat acaaagaaga gcgttcgggt
      961 gatatccatg actctctagc agatattact aagatccagt caattggcta tcaaccgaaa
     1021 tatggtattt taagtggaat gactaattat ttgaaaactg aaatcaatta aaacatacta
     1081 agataagatt agagggtaaa ttcaggctct ttgtcaataa ggactgatga gttttgtaaa
     1141 atcaaatctg ggatagaa
//
LOCUS       DACDIG010000199         1158 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.207, whole genome shotgun sequence.
ACCESSION   DACDIG010000199 DACDIG010000000
VERSION     DACDIG010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.207"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            191..445
                     /locus_tag="IV247_002957"
     CDS             191..445
                     /locus_tag="IV247_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP8778944.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="IV247_002958"
     CDS             446..763
                     /locus_tag="IV247_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP8778945.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          409 a          176 c          231 g          342 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaaggcaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accgtaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1141 gaggaagtcc aatatatc
//
LOCUS       DACDIG010000200         1143 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.208, whole genome shotgun sequence.
ACCESSION   DACDIG010000200 DACDIG010000000
VERSION     DACDIG010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.208"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..888
                     /locus_tag="IV247_002959"
     CDS             <1..888
                     /locus_tag="IV247_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAP8778946.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
     gene            complement(928..>1143)
                     /locus_tag="IV247_002960"
     CDS             complement(928..>1143)
                     /locus_tag="IV247_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8778947.1"
                     /translation="RVRADGIYRPDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRA
                     TNDPEGNTKGGAQDFHDKRWYLNIPQR"
BASE COUNT          449 a          169 c          192 g          333 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt caacttttgg
      901 ggttcagttc acatcgaggg gcttttttta tcgttgcgga atatttaaat accaacgttt
      961 gtcatggaaa tcttgcgcac cgcctttagt gtttccttct ggatcattcg ttgcccgcat
     1021 catgacgtat actttcttat tagggaaatt gcgcatgtta aaagatacat gatagccaac
     1081 gtttccagaa gtattataag cttgatttac atccggtcta taaattccat cagctcttac
     1141 tcg
//
LOCUS       DACDIG010000201         1142 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.209, whole genome shotgun sequence.
ACCESSION   DACDIG010000201 DACDIG010000000
VERSION     DACDIG010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.209"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            9..422
                     /locus_tag="IV247_002961"
     CDS             9..422
                     /locus_tag="IV247_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAP8778948.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            559..819
                     /locus_tag="IV247_002962"
     CDS             559..819
                     /locus_tag="IV247_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778949.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            937..>1142
                     /locus_tag="IV247_002963"
     CDS             937..>1142
                     /locus_tag="IV247_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778950.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDS"
BASE COUNT          363 a          173 c          200 g          406 t
ORIGIN      
        1 gatcaaaaat ggcaaatgta cctagcattc gcgacatgtt ccctgatttc aatgacgtgt
       61 ttagtccagc attcaatgat tttttaggtg tttcaagtta tccaaaagta gatttagtcg
      121 aaaatgaaaa agaatacaaa ctaactgctg atatgcctgg ctgtgacaaa gaagacacga
      181 cagttgaata ttcagataat actttgacga tctccgccaa tcatgagtcc catacagaag
      241 ataaagaaga tggcaactat gtacggaaag aaagacattc ggtctcttac aaacgttcat
      301 tctatcttcc taatgttgat gaagaaaaaa tcactggaac ctttaaaaac ggtgtgctga
      361 aattggtatt acctaaaaca gcttatcagc caaaagaaac aaaaaaaatt gagttaaatt
      421 agaacttatc gcctctcgat gaatgagggg cgatttttat atatttggat aagaacgagt
      481 atgtttaaat tttcaaaatg agcaaaaaaa tattttgttt ttacgataaa gatgttatga
      541 tctctcttgg taaaaaatat gggagttgat catttgattg tcttaagtaa acttttgttt
      601 tggataccat ttatcataat gaccagtgct ttcttttact tattcaaaag gcctaacaaa
      661 tacttactct tatctgcttg tccgatgatc tattttatcc ataagattag aggattttat
      721 tattacgact ctggtatctc attgttttgc tacgaaatcg gcttattagc ttcggccatc
      781 tttttcatat ttgtgttaga atatttcaga aaaaaataaa cttccagtca gttttggctg
      841 gaagtttttt atttggtgca gatcttttca gctgttattc atacaagttg atccattttt
      901 ctattattaa gtacatacca ttgttactct ttgaatgtgg gggagaaaaa aataaaagga
      961 gctggtattt tgttagttat gagtaaagct ttgtttttac ttccctgcac gttcatgttt
     1021 attgttttat gttatttcaa aagaagaaaa aaatggtgct ggatcgatgt tctactattc
     1081 cttccctttt tcccgcttat ttattttctt gaagaaatat gggatgatat ggcatgtgat
     1141 tc
//
LOCUS       DACDIG010000202         1140 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.210, whole genome shotgun sequence.
ACCESSION   DACDIG010000202 DACDIG010000000
VERSION     DACDIG010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.210"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..572
                     /locus_tag="IV247_002964"
     CDS             <1..572
                     /locus_tag="IV247_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP8778951.1"
                     /translation="HAWEIMKKNDPQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMD
                     ETGTYVSVSVTVKGHTETELFPVLDYRNQPVVKPNAMQINNSLKRCFVKALALHGLGL
                     YVFQGEDIPTPPRIDTKKLNMLETILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADN
                     VETIEQLSYEQCALMERAIAAKRKELDKK"
     gene            578..>1140
                     /locus_tag="IV247_002965"
     CDS             578..>1140
                     /locus_tag="IV247_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778952.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIGATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAA"
BASE COUNT          399 a          199 c          236 g          306 t
ORIGIN      
        1 ctcacgcatg ggagattatg aaaaagaatg atccacaggc aacggtaact attaatgagt
       61 ataaacacta cagagttgtt tctggaactc atcaagactt tcttgttgag gaatataaac
      121 cttttcttat ggatgaaact gggacttatg tatctgtctc agtaacggtt aaaggacaca
      181 cggaaaccga attatttcct gttttagatt atcgaaacca accagttgtt aaaccaaatg
      241 caatgcaaat caataactca ttgaagcgat gctttgtgaa agcattggct ctacacggac
      301 tgggattata tgtatttcaa ggggaagata ttccaacacc acctagaatc gatacgaaga
      361 aattaaacat gctagagacg attctagaag ctttcaatga gcagatgggt aaagatatga
      421 ccaaaacctt aatcgaatat gttaatgagc agacagataa attagggctc ttagctgata
      481 acgttgaaac tattgaacag ttaagctatg agcaatgtgc cttgatggag cgagcaatag
      541 cagctaagag aaaagaatta gataagaagt gatatgagtg tttaaaccat taatcgattc
      601 atattcagcg gttctgaaaa agttcaaagg aaaagacata ggtgcaacca tcaatgagga
      661 agtgaacatt gatcgactaa agacgatgta tgacggctac gatggtgatc gagttattga
      721 aattcgtttt attgatccta gacgtttcac agtacagcaa cgaaacttca tctatgcact
      781 cataggcgat attttcatcg atacaggcat gccaacggac ttctggaagg aattctttta
      841 cttccgcttt gaaggtgtca cagggcgcaa aataagcctc aaagatgaat cgaatacgac
      901 tgtgagtgat gctaacgtct tagcaaatat catcttagat ttcatctttg aacatcatat
      961 tcctttcaaa gaaggctatg agattttacc agcgaatcaa gaatattact tctacaaatg
     1021 catcacaaaa agagtctgtt gcatctgtgg caaaacagga gctgatatcg atcactttga
     1081 caaagcgcta ggaagacgaa agcgcaaaga agttgatcat tcagagtaca catttgcagc
//
LOCUS       DACDIG010000203         1124 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.211, whole genome shotgun sequence.
ACCESSION   DACDIG010000203 DACDIG010000000
VERSION     DACDIG010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.211"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>1124
                     /locus_tag="IV247_002966"
     CDS             <1..>1124
                     /locus_tag="IV247_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HAP8778953.1"
                     /translation="IKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHTCRNC
                     GSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQSYFV
                     QPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSKRILP
                     RVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEEVKFL
                     VTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKSQAEK
                     LKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAYPYFH
                     ELVEAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYE"
BASE COUNT          363 a          217 c          222 g          322 t
ORIGIN      
        1 tatcaaaaaa atgctgcgat taatagagaa agatttgatg attacagagg tctcttacga
       61 gacctttcag aagaaaaaga cgttgatcgt cgatgctgtt ttctcacctg ctccccatac
      121 ctgtagaaat tgtggttcta ctgtggtaga tggaaacggg aaagtaattg tagtgaaaaa
      181 tgggaagaag gaaacgattg tccgctttga acaatacaat cacatgcctt tggttatgcg
      241 cttaaaaaag cagcgctata cctgtaaaaa ctgtcgaacc cattggacgg ctcaaagtta
      301 ttttgttcaa cccagacatt caatcgcgaa tcatgtcaga tataaaattg cttctttact
      361 gactgaaaaa gtatctttat cttttattgc gaaaaattgt caggtatctt tgaccaccgt
      421 tattcgtacg ttgaaagagt ttaaaagcta cttaccaaag caatctaaga ggattctccc
      481 cagagtattg atggttgatg aatttcgttc gcacgcttcc atagaagata aaatgagctt
      541 tatttgcgca gatggcgaaa caggacaatt aattgatgtc ttgcctacgc gtaaattgcc
      601 tcgattaaca agctatttct taggctgcgc taatcctgaa gaagtaaaat ttttagtcac
      661 agacatgaac gccgcctact tccagcttac caaacgtatt ctgccaaatg cgaaagtagt
      721 gattgatcgg tttcatatta tcaaacacat gaatcaagcc tttaatgagt tgcgtatccg
      781 tgaaatgaat gaacttcgta aagtaggaca gaaaagtcag gcagaaaaac tgaaaaagaa
      841 ctggcgcttt ttgctaaaaa atcgtgcaaa catcaaccat tatgaataca aaacatggaa
      901 aagttttcga gcaccaaaat acccatttct tacggaagcg atgatgattg atcgattgct
      961 tggcttttct acgtccctga aggaggcgta tccctatttt catgaattag ttgaagcctt
     1021 tcgagacaaa gaccctgact tatttttctc cttattggca gaacttcccg aaacgttaga
     1081 tgacggcttt cgggaaaagc tccaaaacct tctgacctat gaag
//
LOCUS       DACDIG010000204         1120 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.212, whole genome shotgun sequence.
ACCESSION   DACDIG010000204 DACDIG010000000
VERSION     DACDIG010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.212"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..704
                     /locus_tag="IV247_002967"
     CDS             <1..704
                     /locus_tag="IV247_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP8778954.1"
                     /translation="LRKGLLEFKLQELELHYSYLVKITEEKEQQKAIKEQMIEEEKVR
                     REIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKELYANKIQELEEKLKELEVIR
                     ENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEPMDRIKELSSASVPFEFDVH
                     AMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLDEIEKVVLENYNGTVTFTKL
                     AKAEQYRRSLELSKD"
     gene            776..>1120
                     /locus_tag="IV247_002968"
     CDS             776..>1120
                     /locus_tag="IV247_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778955.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQD"
BASE COUNT          491 a          130 c          187 g          312 t
ORIGIN      
        1 aattaaggaa gggtctttta gagtttaaac ttcaagaact agaacttcat tactcttatt
       61 tagtgaaaat taccgaagaa aaagaacaac aaaaagcaat aaaagaacag atgatagaag
      121 aggaaaaagt tcgtagagaa attgatcgtg aaaagaaacg tattgataaa gaacaacgtc
      181 aattcaattc tgaaatttcc aaattaatcg catatatgca aaaagctaat gcagatgtgg
      241 aaaaagaatt atatgcaaat aaaattcaag aactggaaga aaaattaaaa gaattagaag
      301 ttatacgtga aaatgtatta caaagagaat taaatactag agccggttat gtgtatgtaa
      361 ttagtaatat tgggtctttt ggtgaagaca tatataaaat tggtatgact agaagattag
      421 aacctatgga tagaataaaa gaattgagta gtgcatcggt tccatttgaa tttgacgttc
      481 atgcgatgat tttttcagag gacgctccgt ctttggaaac taaattgcat aatcatttta
      541 gaaaacaaga agttaataaa attaatcaaa gaaaagaatt tttcaaagtt tcactagacg
      601 aaatagagaa agttgtttta gaaaattata atggaacagt aacatttaca aaattagcta
      661 aagctgaaca atatagacga tctttagaat tgtcaaaaga ttaaatatat aaaaagcctt
      721 cgggcttttt cttttaacaa ttaataggaa cgtatattcg aaaggaatag aaataatgtg
      781 gttagaaaaa ataaatgaca aaaaatttaa gtacaccgaa cgttatacgg atcccttaac
      841 tgaaaaaaag agaaaagtgt ccgttacttt atctagcaat tcacgccaag cttggaatca
      901 agctaactta cttttgaatg aaaaaatcgc agaaaaaata aaacgaaatg aagaattacc
      961 actaactttt ggagaattaa aaactaaatg ggatgaaaaa tacaagccaa ctgtaaaaga
     1021 aagttcctat cgaacaactc aggtttatct atcattaatt tcaaaatata taaaagatga
     1081 tgtacttgta aaaaacgtta atagtaatct tatacaagat
//
LOCUS       DACDIG010000205         1106 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.213, whole genome shotgun sequence.
ACCESSION   DACDIG010000205 DACDIG010000000
VERSION     DACDIG010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.213"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            1..999
                     /locus_tag="IV247_002969"
                     /pseudo
     CDS             1..999
                     /locus_tag="IV247_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          391 a          199 c          222 g          294 t
ORIGIN      
        1 agaaaaagat aatcttacgc ctgaacaaaa agaattgtta gaactacgta aaagaaacca
       61 gcaattagaa atggaaaatg atattttaaa gcaagcagcg ctgatattcg gacgaagaga
      121 caagtaatcg atgcgaataa gcatctttac cctatatcag cgatgtgcag aatattaggt
      181 ctatcacgtc agtcctatta ttatcaatca aaaccaaaga aagacgaatc agaacttgaa
      241 gaagtagtcg ctgaagaatt tatccgcagc cgaaaggcct acggctcaag aaaaataaaa
      301 aaagccttat caaaacgagg cattcagatc agccgacgaa aaattagtag aatcatgaaa
      361 aatagaggat taaaatcgag ctatactgtt gcttatttta aagtacatca ttctacttgc
      421 aatgaagcca aaacgacaaa cgtattgaat cgtaaattct taagagacaa cccattagaa
      481 gcgatcgtaa cagacttgac ttatgtacga gtcgggaaaa aatggaatta tgtctgtttc
      541 attttggatc tgttcaatcg agaaattctc ggctattctt gtggagaaca taaagatgcc
      601 gttctagtaa aaaaagcatt tagccgtatc aaacaacctc tgacagaggt tgagattttt
      661 catactgatc gtggaaaaga gtttgataac caagctattg atgaattatt aacaactttt
      721 gacatcaatc gatcattgag tcataaaggc tgtccttttg ataatgccgt agctgaatca
      781 acttataagt cgttgaaagt agaatttgtc tatcaataca catttgaaac cttacaacaa
      841 ttggatttgg agttatttga ctatgtcaat tggtggaacc accttcggtt gcacggtaca
      901 cttggctacg agacaccggt tggttaccgt aaccagagat tggcgcagcg aatccttgat
      961 aatgagctcg gatgtgctaa cgctagcgag gcagtctaac tttaactgtt agctcctgcc
     1021 gaagatcgtc acatccgagg aggctcattg tcaaggacaa tcggaatagc atacggaaag
     1081 aagttctgca ccttataaaa tttgtc
//
LOCUS       DACDIG010000206         1105 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.214, whole genome shotgun sequence.
ACCESSION   DACDIG010000206 DACDIG010000000
VERSION     DACDIG010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.214"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            177..971
                     /locus_tag="IV247_002970"
     CDS             177..971
                     /locus_tag="IV247_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4428 domain-containing protein"
                     /protein_id="HAP8778956.1"
                     /translation="MSKQCAKCGGKIGLTSYKIKDKQIVCGNCMKKAGYGMTTPYKII
                     RSLNLEDLDKGPSEKRKLNISDYTTQNNHDIGNDRMRTTEEMVEFCLKYGYGKGMTKK
                     WTTHHFNLISDQLNSDEYVIFCFVGLHNYISSTKHDNNYAYALTNKRLIVAQQKMIGN
                     NVQSIILDNLNNISKKREILLGTLTIDTLGKVLNVAVDKDTVDRISDSLNEIIYNLKR
                     RNISAINSNTSTISSASEIRKYKELLDDGIITEEEFNKKKQELLDL"
BASE COUNT          444 a          132 c          170 g          359 t
ORIGIN      
        1 aattatactg tttcctgata agctctcaat gcttctgaac ccatgatatt agtgcttttc
       61 ttctattatt aaatatcttg taattttcta ttctttctaa aaagtatggt caaagtatgg
      121 tcactattga tagaatatta atagtagtag aaagctgata tctaggagga actaatatga
      181 gtaaacaatg tgcaaagtgc ggaggtaaaa ttggcttaac ttcatataaa atcaaagata
      241 aacaaattgt ttgcggcaat tgtatgaaaa aagctggtta cggtatgact actccttata
      301 aaataataag atctttgaat ttagaagatt tagataaggg tccatcagaa aaaagaaaat
      361 taaacatatc tgattatact acacaaaaca atcatgatat aggtaatgat agaatgagaa
      421 ctactgaaga gatggtagaa ttttgtctta aatacggcta tggtaaagga atgactaaaa
      481 aatggacaac tcatcatttt aacttaatat cagatcaact taacagtgac gaatacgtaa
      541 tcttctgctt tgtaggattg cataactata tctcgtcaac taagcatgac aataattatg
      601 catacgcatt aactaataaa cgtttaattg tagctcaaca aaaaatgata ggcaataatg
      661 tacaaagtat tatattagac aatttaaata atattagtaa aaaaagagaa atactgttgg
      721 gtacattaac tattgatact cttggtaaag tattgaatgt tgcagttgat aaagatactg
      781 ttgataggat aagtgattct ttaaacgaaa ttatttataa tttaaaaaga agaaatattt
      841 ctgctataaa ttccaataca tcaacaattt cctcagcttc agaaataaga aaatataaag
      901 aactattgga tgacggaatc attacagaag aagaatttaa taaaaagaaa caagaattat
      961 tagatttata atatctatca aaaaagatga gcatttaatt tagctcatct ttttctattt
     1021 acaatacaaa catttgttcg tatactcttt ttgaggtgaa cattatgttg atggaaggaa
     1081 aaacacaatt atggtttaaa tttga
//
LOCUS       DACDIG010000207         1084 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.215, whole genome shotgun sequence.
ACCESSION   DACDIG010000207 DACDIG010000000
VERSION     DACDIG010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.215"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..626
                     /locus_tag="IV247_002971"
     CDS             <1..626
                     /locus_tag="IV247_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP8778957.1"
                     /translation="LYEANRDNLTWSTKSLDRLKNPVFSIADLRKKFSIEKKYKAFRD
                     FERRVIKEPIAQLNKYTDFKIDYELIKKGHTYTNIKFLLTRTMSVPVPYKQEQRDPAY
                     LKGAEDKTEKMNQSYLKAMNSKYTKLLIKNFLISSADLCDLKLMSRLQNKVYPLYDRL
                     SKDRGFESVVNHLSYVATHKEDYTIKNTVKYLEKSITSYLPYVNLYD"
     gene            661..>1084
                     /locus_tag="IV247_002972"
     CDS             661..>1084
                     /locus_tag="IV247_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP8778958.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASF"
BASE COUNT          411 a          169 c          177 g          327 t
ORIGIN      
        1 atctgtatga agcaaataga gataacttaa catggagtac aaaatcactt gatagattaa
       61 aaaatcctgt tttctccatt gcagatttac gaaagaaatt tagtattgag aaaaaatata
      121 aagcttttcg agactttgaa agacgtgtaa ttaaagaacc gattgcccaa ctgaataaat
      181 acactgattt taaaattgac tatgagctta tcaagaaggg tcatacttat acaaacataa
      241 agttcttgct tacacgtacc atgtcagttc cggtacctta taagcaagaa caaagagacc
      301 ctgcttattt aaaaggcgca gaagataaaa cagagaaaat gaatcaatcg tacttgaaag
      361 ctatgaatag caaatacact aaattactaa tcaaaaactt tttgatcagt tctgccgact
      421 tatgcgatct taaacttatg agccgacttc agaataaagt ttatccgctt tatgatcgtt
      481 tatccaaaga tagaggattt gaatcagttg tcaatcacct atcttacgtt gctactcata
      541 aggaggatta cacaataaaa aatactgtta aatatctcga aaaatcaatt acgagttatt
      601 tgccttatgt gaacctttat gattaacaac tgaaaaaatg cgtgaaggaa ggaaaaactt
      661 ttgaaaaaag atgaactgac aaagtacaat gaggagttct tagcaacaat tgacatgaaa
      721 atggatttaa agtcgtatga actttatcaa ttgctgttca gttatatctc acccacaaag
      781 ctaaagaaaa caggcgtagt agatttacct gttaaagcga ttattaatta catggatgtt
      841 gatcaaaatc ttgaaacgga actaaaaacc atagttaaca ctttgcaaag acaagcagtt
      901 tatgaaattt cgaatctaga caaaaaatat aagacgtata ctactgtatc tgctattgat
      961 cggattcatt gggatttaga agagcatagt gtatctttca gtttttcaga agatgtaatt
     1021 cccttaattg tctcaatcaa aaagaagtat ccgttctttg tttttgaaga cttagcatct
     1081 tttc
//
LOCUS       DACDIG010000208         1062 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.216, whole genome shotgun sequence.
ACCESSION   DACDIG010000208 DACDIG010000000
VERSION     DACDIG010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.216"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(36..992)
                     /locus_tag="IV247_002973"
     CDS             complement(36..992)
                     /locus_tag="IV247_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778959.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          178 c          160 g          378 t
ORIGIN      
        1 tagaaaaagc agtcggactt tctaaaagaa gttcactatt cgatcctgtc gatgatttct
       61 tcaggaaaat cgtctatttg cttgtcaatt ttgctgtctt tgtatttctc ttcttggata
      121 gtcaacaaaa tctttgtaag attcccccag tctactgctc ttggcggatc gagccaaacc
      181 actacttgag atgcactttc ttcatataga ttattcctat tcgtgattat aacatccggg
      241 aactcaggct ttcctgcctc tatcacttcc gtgttcagat taatgtacaa gcttaaattt
      301 ttcttgataa attgattata ggcagcacca taagaaaaat cgatgatgat ctttactggt
      361 tcagaaatca gttgaatggg tatgtgattg atcaataata ataaataact gaaaaaaaga
      421 aaaaatttat cttgaagcaa ctctttgcgt tttgacgtta gatcatttat atactctatc
      481 aaatagcaat aaaatgctgg atagttttct ctaaaaaaag aaacatcaag tggaaattca
      541 tcagctttat taatagtgta atgcaaaagt ttaaaatgta ttttggttaa ctctcttcgg
      601 atcaattggc tcgtctcttc tcgcaacaga ttaaactgat tttcaaaact ttcaatgaat
      661 cgttcattca accattcgat tgtttcatct tcattttttc tcaagtattt ttgatccaat
      721 ttatcatgaa gataaagcca agagagtgtc ttattgataa aaataacgga tttttctcct
      781 aatactcctt ttaagtggta aaatcctata tccgaaaaaa atagcgactg attgctagca
      841 atatctaaag aatcagcttg ttcagccaac aggttgattg ctaaaaagtg gaataatttc
      901 tttttttcat aagcagacag aggataatac tgttttaaat cttttaacat tttttctgtt
      961 tttataatta taggtagcgt atacaattcc acaaaactta tctctgttga gttaaaaaga
     1021 ttatataaaa aaagccgaat gtttttagga ttccctgtca ac
//
LOCUS       DACDIG010000209         1059 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.217, whole genome shotgun sequence.
ACCESSION   DACDIG010000209 DACDIG010000000
VERSION     DACDIG010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.217"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            111..1046
                     /locus_tag="IV247_002974"
     CDS             111..1046
                     /locus_tag="IV247_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778960.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          395 a          123 c          191 g          350 t
ORIGIN      
        1 agactattta caacatttta ctttgtacgt attcttgttt taaaaggaat gggataaaat
       61 ataggtacaa taactatttg atgctaaata taattaatgg aggagagata gtgaacatac
      121 ttaatgaaaa aagatatgta tggtttgctg gtaaaaaata tattactact ttcaaaagat
      181 tatccgagtt aatgcagcag actgttaaaa gtgctatgcc tgaagggtac aagtttaatt
      241 ttgttcaatc ttcagtatct actgaaagta tctatttttc aattctttct gagagtaggc
      301 tttttcattt tagaataagt tctcatgtaa agaaaactga tgttgatata tatacgttcc
      361 gtacaaataa ttataaaaat ttaaatgatt tgagtagaag tatcagtgaa tatctcaagg
      421 ggaatagcta tctcaagatt cgattttcac atatattaat tttactacca tatttgaatg
      481 agaatttcac cctaattcaa gtagataagt ctttcattgc ctcaggtaat caaggaaata
      541 tgaatattac aagtaacaaa acgtcacgtg tgctattggc gttgatgggt tacggaattc
      601 tttatagaga tgaccaaaat ggccagattc taataaatcg ggctgggcgt tcattattaa
      661 tgaaatatag aaaatttata ccggaagatt ttcaaaactt acagttagaa aacatgcgtt
      721 tagaagattt atttagatta gcagaagatg ataagtatag atttgaagtt ttgaaaattc
      781 aaaagaatga actattgaat aagttatatc ctgagaatgc gataaaagca cattttggtg
      841 atgctaatca tatgtatagt tggttcattc ctaaaagatt tagagggaaa atcaatgctg
      901 gagatcaagt gaaagtactc aatcgaaata gtacagatgt cgtaatgata acagatatct
      961 atgtagaaga tgaagatata atcaaatata tgaagcctgt gataaaactt gttaataaaa
     1021 ataaaggaac ccgtggtgct agttaatttt ttcctagag
//
LOCUS       DACDIG010000210         1050 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.218, whole genome shotgun sequence.
ACCESSION   DACDIG010000210 DACDIG010000000
VERSION     DACDIG010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.218"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            4..978
                     /locus_tag="IV247_002975"
     CDS             4..978
                     /locus_tag="IV247_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP8778961.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTITPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          376 a          133 c          180 g          361 t
ORIGIN      
        1 actatgtgta cgtctattac ttatgtaaca agtgatcatt attttggaag gaattttgat
       61 tatgaaatat cttacaatga agtagtcact attactccaa gaaattataa gttgaatttt
      121 cgaaaggtaa atgatttgga tactcattat gcaatgattg gtattgccgc tggtatagct
      181 gactaccctc tttattacga tgcgacaaat gaaaaaggat tgagtatggc tggactaaat
      241 ttttctgggt atgctgatta taaagaaata caagaaggga aagacaatgt atctcctttt
      301 gaatttattc cttggatttt aggacaatgc tcaacagtag gagaagctaa aaaattgtta
      361 aaaaatatca atttagcaaa tataaattat agtgacgaac ttcctttatc ccctttacat
      421 tggctattag ctgataaaga aaaatcaatt gtcattgaaa gtatgaaaga tggacttcat
      481 atatatgata accctgtggg cgttcttacc aataatcctt catttgacta tcaattattt
      541 aatttaaaca attatcgtgt cttatcgagt gaaactccta aaaataattt ttcaaatcaa
      601 ataagtttga atgcctatag ccgcggtatg ggagggatag gcttgcctgg agatttatcc
      661 tcagtatctc gttttgttaa agcgactttt acgaagctga attctgtatc tggagattca
      721 gagtcagaaa gtattagtca atttttccat atcttaggtt cagtagaaca acaaaaaggt
      781 ttgtgtgatg ttggtgatgg aaaatatgaa tatacaattt attcttcttg ttgcaatgtt
      841 gacaaaggaa tctattatta tcgaacatat gaagacagtc aaattactgc aattgatatg
      901 aataaagaag acttagatag tcataagtta attagttatc caattataga aaaacaacaa
      961 attaaatata taaattagtt aatgtgttgt gattgattat ttaatataga tataatacaa
     1021 aaaggcaaag atattttcaa aacatttttt
//
LOCUS       DACDIG010000211         1035 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.219, whole genome shotgun sequence.
ACCESSION   DACDIG010000211 DACDIG010000000
VERSION     DACDIG010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.219"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            42..521
                     /locus_tag="IV247_002976"
     CDS             42..521
                     /locus_tag="IV247_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778962.1"
                     /translation="MTDKTSDLPKLAPKTSRVKSKKDGKTKNAVKKKSVKETSSLLLD
                     IPMSSFDDNTDETSVKIATHVGMNNQNDSFYNLLNSLRELEDKKNQVIASKAPDLTTD
                     LDAEIIQIKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            complement(725..955)
                     /locus_tag="IV247_002977"
     CDS             complement(725..955)
                     /locus_tag="IV247_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778963.1"
                     /translation="MWNNRLKTGLLLIVISCAMMIGMRIQREQSYFEVSANNVIEKCY
                     YGQHYWSEEVRENIDREYVQRIVRLPVSELKK"
BASE COUNT          392 a          202 c          126 g          315 t
ORIGIN      
        1 taatataata acataaatta atagaaagga aaagctaata aatgactgat aaaacaagtg
       61 atttacctaa actagctcca aaaacatctc gagtaaagag caaaaaagat ggaaaaacaa
      121 aaaatgctgt aaagaaaaag tcagtaaaag aaacatcctc gttactactt gatattccaa
      181 tgagttcttt tgatgacaat acggacgaaa cttctgttaa aattgcaact cacgttggaa
      241 tgaacaatca aaatgattct ttttacaact tgttaaacag cctaagagag ttggaagata
      301 aaaaaaatca ggtaatcgca tcaaaagctc ctgatctcac aaccgatttg gatgctgaaa
      361 ttatccaaat caaacaagat gagcgatcag ttaaatcgct actgaaaacc tactttgatg
      421 gttttgataa agaactagct ccttatagcc aagcaatcct aactaaacca atacaagctc
      481 cacatatttc aatttcccta caaaaaaaga gtgatgatta aacaatcatt actctttttt
      541 agatttctgg taagcgctga tcgcaaaaac catcaacaat attgattcaa aaaatatgtt
      601 cattcgtgtc tcttttttcc attatagatt tcccactatt ccattaaatc gaaagtcaca
      661 ccgattacta caaagattaa cgtgaccaca ttcaaaaaca ttaaaagata tgctgataat
      721 ctcatcattt ttttagttca cttactggta atctaacaat tctttgaaca tattcacgat
      781 ctatattttc tctgacttcc tcactccaat aatgttgacc gtaatagcat ttttctatta
      841 cgttgttagc agaaacttca aaataggatt gttccctttg tatcctcata ccaatcatca
      901 tagcgcagga aataactatt aataacaatc ctgtttttaa tctattattc cacatgcagt
      961 ttccacatcc ttatcttgat aaaaatcctc ttgaccctct ccaattactg ttttaaatat
     1021 aaccaccaaa acaag
//
LOCUS       DACDIG010000212         1033 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.220, whole genome shotgun sequence.
ACCESSION   DACDIG010000212 DACDIG010000000
VERSION     DACDIG010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.220"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..358)
                     /locus_tag="IV247_002978"
     CDS             complement(<1..358)
                     /locus_tag="IV247_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778964.1"
                     /translation="MSQNISAVKQLIEEDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAKEKSSILELSQ
                     NDKVIQVKCVDPTGKII"
     gene            complement(374..739)
                     /locus_tag="IV247_002979"
     CDS             complement(374..739)
                     /locus_tag="IV247_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778965.1"
                     /translation="MSSLKDDYIQAKQEYEKIQMLKSEKVELFLTQKLAEDHIANLDA
                     KTLEEYDRQFNQIHHNIILLVEQAKRRFEKAEYDLLDQGIVCLDKGLRRYYGAELKKQ
                     NVDRNALKHEILELIMQRI"
     gene            complement(745..>1033)
                     /locus_tag="IV247_002980"
     CDS             complement(745..>1033)
                     /locus_tag="IV247_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778966.1"
                     /translation="FRKLKGIKQAEIALYEMKKVDRLKLIQDLVQNTWNQAIVELEKE
                     NGKILTNKYLIVNEIEELIGLFSCEKGEVFLKSSPTYSILDSPYQFIQARR"
BASE COUNT          281 a          205 c          131 g          416 t
ORIGIN      
        1 caataatctt tcctgttgga tcaacgcatt tcacttgaat aactttgtca ttttgcgata
       61 gctctaatat tgatgatttt tcttttgcct cttccaaaag atgttctaac acttcgttag
      121 gtgttccatg atagtaataa atcatggtct tctcgaaaat ttttatcgga tcaacaccat
      181 attcttttcc gattcgataa aaaaatttca ttggaatttc ttccggattt ctgttcctgc
      241 ttgaccagtt agagatggtt tgataatttg caaaatggat tcgttccgct aatctctgaa
      301 ctgtggtctc taaatcttct tctattaatt gttttacagc actaatgttt tgtgacattt
      361 gctttctatc ccttcaaatt cgttgcatta tcaattctaa gatttcatgt tttagtgcat
      421 ttctatctac attttgcttt tttaactctg caccataata tcttcgcaat cccttatcca
      481 agcatacaat tccttgatct aatagatcat attccgcttt ttcaaacctt cgctttgcct
      541 gctcaacaag caaaatgata ttgtgatgta tctgattaaa ctgccgatca tattcctcta
      601 aagttttagc atctaagtta gcaatatgat cctctgctaa tttttgtgtt aaaaataatt
      661 cgactttctc agatttcaac atctgtattt tttcatactc ttgttttgct tggatatagt
      721 catctttcaa gctagacatc taactcatct ccttgcctgt ataaactgat acggactgtc
      781 caaaattgaa taggttggtg atgacttcaa gaagacttca cctttttcac atgaaaataa
      841 accaattaat tcttcaattt catttacgat cagatattta ttagttaaaa ttttcccatt
      901 ttctttttct agctcgacaa tcgcttgatt ccatgtattt tgcactaaat cttgaatcaa
      961 tttcaatcta tccacttttt tcatctcata cagagcaatt tcagcttgtt taatgccttt
     1021 taatttgcga aag
//
LOCUS       DACDIG010000213         1017 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.221, whole genome shotgun sequence.
ACCESSION   DACDIG010000213 DACDIG010000000
VERSION     DACDIG010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.221"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(602..675)
                     /locus_tag="IV247_002981"
     tRNA            complement(602..675)
                     /locus_tag="IV247_002981"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(639..641),aa:Ile,seq:cat)
     gene            complement(865..>1017)
                     /locus_tag="IV247_002982"
     CDS             complement(865..>1017)
                     /locus_tag="IV247_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8778967.1"
                     /translation="VGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWY
                     LNIPKR"
BASE COUNT          339 a          178 c          175 g          325 t
ORIGIN      
        1 gactgacgtg ccggacgcac gtagcgatca tcggcgttat gactatataa agtatacttt
       61 cgttgatcca gaaaggtata aagattaaag taggctacct aaaaaggtag cccggaacga
      121 attttatcac catacttgtg aaaggagata ttttttaagt tagtattaag attgtgtaat
      181 atgatgatat ctatatttta tagtatcagt gctataaaat caaaaataag tcactaatta
      241 actaccactc caattgtaag ccttttttct cacttttttt caaaaatatg gtatgctttt
      301 taatggcttc aaatataaaa gagtttaaag cgtaacacac ttatggggaa gtggtttggg
      361 gtgcgcttta aactcttctt tattattatc tcacaattta acccaaatgt ctttctattt
      421 aaaaatcaaa gtaaaacttt tcaaatatac agaagtataa ctatgtgaaa catcctttca
      481 ttaatccata aaaggataca taaaaaagcc actcatttga gtggcaatga agaaaagctt
      541 tagcttgtat aatactcttc aaaaaaattc taacacagaa cgattcaaat ggctacgtta
      601 atgtaccctg taggactcga acctacgacc ggacggttat gagccgtctg ctctgaccaa
      661 ctgagctaag ggtactggtt gttgccacat aaagccataa acaatcaacc agtagaatgt
      721 gtggcaacaa acctgttatc gcatagcttg gagtgtgact atttatgggt gatagtgaag
      781 atatgcgata acatcactat tttatcgaat aatttttata gttgtcaata tagttatgta
      841 ctgctcctca acgaggagct atttttatcg tttaggaata tttaaatacc aacgtttgtc
      901 atggaaatct tgcgcaccgc ctttagtgtt tccctctgga tcattcgttg cccgcatcat
      961 tacatagact ttcttactag gaaaattacg catattgaaa gatacatgat aaccaac
//
LOCUS       DACDIG010000214         1002 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.222, whole genome shotgun sequence.
ACCESSION   DACDIG010000214 DACDIG010000000
VERSION     DACDIG010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.222"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            369..1002
                     /locus_tag="IV247_002983"
                     /pseudo
     CDS             369..1002
                     /locus_tag="IV247_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          182 c          183 g          295 t
ORIGIN      
        1 tttcaaagtt agtagagaaa atgtagttat atatttaaat ttttaccaga aagaaatgaa
       61 caccttcaat tatctaaatc tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt
      121 aaacgaatta ctattcatgg tcttagacac acacatgcta gcttgctttt agcaagctag
      181 tgcaaatatt aaagatgtgc aagagcgttt aggccactct tctattcaaa tcattatgga
      241 tctatatatt catattacag acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaata
      301 taggctctat aatttatggg actgatgaat caaaattgat tcttcagtcc cattgttatt
      361 ttaggtatat gattcgtaca ttgaaagagt tgaaaaacta tcttccgaag ccgtcaaaaa
      421 ggaccctgcc aaaagtattg atggtggatg aatttcgttc gcacgcttca attgtggata
      481 aaatgagctt tatttgtgcg gatggcgaga ctggaaaact cattgatatt ttgcctacac
      541 gaaaattgtc tcgattaaca cactatttta gtcgctgtgc aaatcctgaa gcggtggaat
      601 atttagtgac agatatgaac gccgcctact tccaactgac aaaacgtgtc ttaccaaatg
      661 cgaaagtcgt cattgattgt tttcatatca tcaaacattt gaatcaggcg ttcaatgatt
      721 ttcgtgtaca agaaatgaaa agattacacc agttagggca acgacgggaa gccgagaaag
      781 tgaaaaagaa ctggcgcttt cttctaaaaa accgtcaaaa aatcaatcac tatgattaca
      841 aaacatggcg cagttttcgt gcacctaaat acccgctact cactgaagca atgatgattg
      901 atcgattgct ggcattttca acgcctttaa aggacgccta tacttttttc catgagctga
      961 ccgaagcctt tagagacaag gaatcagact tattttttac ct
//
LOCUS       DACDIG010000215          976 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.223, whole genome shotgun sequence.
ACCESSION   DACDIG010000215 DACDIG010000000
VERSION     DACDIG010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.223"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            188..955
                     /locus_tag="IV247_002984"
     CDS             188..955
                     /locus_tag="IV247_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAP8778968.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIREKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
BASE COUNT          412 a          136 c          180 g          248 t
ORIGIN      
        1 taagaacgca ttctaatcac tcccgacaag gcaagacagg cgaaaaagat aaataaatga
       61 acgaaaggaa gaaacataaa atgcaaaacc aaatagctat gaagaaaaaa acacctccct
      121 ctaaaacaat ccaaacaaaa aagaattttg aaaaaccaaa aatcaaaaac agaaagaagc
      181 gttcaacatg agagatagaa ttttagaagt taatcaagaa atggtaaaca agttaacaaa
      241 tatcagtggg aactatgaaa agtacgatgg tactttactt gataactata tttttcacga
      301 tacacaaaat atcagaattg gcagaagtaa accgagagcg ttcattctca tcagagaaaa
      361 atatttgaat gaatggaata gcacctatga aatggttatg actgataact ttgaaaaggt
      421 attggatttc agaaatgctt tgttgggttc tgatatggga aatagcagtc ttgaatctat
      481 tgaagttgaa aacaagaccg agaatgaagg tgaaggattg attaagaatg tcttgaaaat
      541 cgaacaaaaa tatatcagtg aagatagaag tttttataga gtcacaagct cggttggtct
      601 agcaaacttt gctactaact taattggaaa caatgctagt gaatctttgc tagtgatgtg
      661 tctgaataca aagaatgagg tcattgctta tagcgaagtt ttcaaaggtg gaattaatca
      721 atcagtagcg ataccaagag agatttttca actagctttg ctaaacaatt cagcaaaaat
      781 cgcagtatca cataatcatc cgagcggata tgaatttcca agtgaaaatg acaaaagttt
      841 tactacgaat ttgaaaaacg caggaaaaac aattggtatt gaattgttag accatatcat
      901 cgtaacaagt aaaaacacaa gctattactc atttcaagaa aatggttctt tataaaaatt
      961 agagaaaaga ggaaaa
//
LOCUS       DACDIG010000216          956 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.224, whole genome shotgun sequence.
ACCESSION   DACDIG010000216 DACDIG010000000
VERSION     DACDIG010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.224"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..932
                     /locus_tag="IV247_002985"
     CDS             <1..932
                     /locus_tag="IV247_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8778969.1"
                     /translation="RNGKFSQQTLPAYKRTNDSLETTIIQLFQKGITMSEISELIEKM
                     YGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVIFMDATHIPLKRQTVSKEAVYIV
                     IGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRGLKEVLLVVTDGLSGINDSIHSV
                     YPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLVYQASSKEEANNQIRFMIDKWKK
                     QYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEGFNKQLKKYTKRKEQFPNEESLE
                     RFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT          348 a          148 c          153 g          307 t
ORIGIN      
        1 atagaaatgg taagttttct caacaaactt tacctgctta taaaagaacc aatgactctt
       61 tagaaaccac tattattcag ctatttcaaa aaggaattac tatgtctgaa atctctgaat
      121 tgattgaaaa aatgtacggg catcactata cgccacaaac catttcaaat atgactaaaa
      181 tagtttctga agatattatt gcctttaaag aaaggtcctt agaatccaga tactctgtca
      241 tttttatgga tgctactcac attcctttaa aaagacaaac tgtttcaaaa gaagcagttt
      301 atattgtaat tggcattcga ttagatggaa ccaaagaggt tcttgggttt agtcttgctc
      361 caactgaatc tgcttatgtt tggaaagaga ttcttcaaga cctaaaagac cgtggtttaa
      421 aagaggtttt attagtcgta actgatggtt taagtggcat taacgatagt attcatagtg
      481 tttatccaaa tgctcaattt caacaatgtt gtgtccatat atctagaaat atcgctcata
      541 aggttcgtgt tagtgatcga caagaaatct gtagcgattt caaattagtt tatcaagcaa
      601 gttcaaaaga agaagcaaat aatcaaataa ggtttatgat agataaatgg aaaaagcagt
      661 atccacgagt ggttaaattg ctcatgaatc ctgctatatt aaccttttat aatttccctc
      721 cgtcaatcag aagaactatt tattcaacta atttgattga aggttttaac aaacaattaa
      781 agaaatatac caaaagaaaa gagcaatttc ctaatgaaga atctctggag agatttctag
      841 tatctcaatt caacaactat aatcaaaagt ttctatgccg agttcataaa ggttttaaag
      901 aaatacatga tacattagaa tcaatgtttt aacttgatca acagaatggg cttttc
//
LOCUS       DACDIG010000217          943 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.225, whole genome shotgun sequence.
ACCESSION   DACDIG010000217 DACDIG010000000
VERSION     DACDIG010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.225"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            2..>943
                     /locus_tag="IV247_002986"
     rRNA            2..>943
                     /locus_tag="IV247_002986"
                     /product="23S ribosomal RNA"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DACDIG010000218          942 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.226, whole genome shotgun sequence.
ACCESSION   DACDIG010000218 DACDIG010000000
VERSION     DACDIG010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.226"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>942
                     /locus_tag="IV247_002987"
     CDS             <1..>942
                     /locus_tag="IV247_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778970.1"
                     /translation="GKSLEALELEQEEIRRFQNCSLYPVYHGSAKSNIGIEQLIEVIT
                     NKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILHLRDSVRISEKEKIKITE
                     MYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLPQRERIENPLPLLQTTVE
                     PSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLGKVQMEVISALLQEKYHV
                     EIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLPLGSGMQYESSVSLG
                     YLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYG"
BASE COUNT          322 a          150 c          201 g          269 t
ORIGIN      
        1 cggtaaatca ttagaagcat tagaactcga acaagaggaa atcagaagat ttcagaattg
       61 ctccttgtac cctgtttatc atggaagcgc aaaaagcaac atagggattg agcagcttat
      121 agaagtgata acgaataaat tttattcatc aacatacaga aagaagtctg aactttgcgg
      181 aaatgtcttc aaaattgaat attcggaaga aagacaacgt cttgcatatg tacgccttta
      241 tggcggaatc ctgcatttgc gggattcggt tagaatatcg gaaaaggaaa aaataaaaat
      301 tacagaaatg tatacttcaa taaatggtga attatgtaaa attgataagg cttattccgg
      361 ggaaattgtt attttgcaaa atgagttttt gaagctaaat agtgttcttg gagatacaaa
      421 gctattgcca cagagagaga gaattgaaaa tccgctccct ctgctgcaaa caactgttga
      481 accgagcaaa cctcaacaaa gggaaatgtt acttgatgca cttttagaaa tctccgacag
      541 tgacccgctt ctacaatatt atgtggattc tacgacacat gaaattatac tttctttctt
      601 agggaaagta caaatggaag tgattagtgc actgttgcaa gaaaagtatc atgtggagat
      661 agaactaaaa gagcctacag tcatttatat ggagagaccg ttaaaaaatg cagaatatac
      721 cattcacatc gaagtgccgc caaatccttt ctgggcttcc attggtttat ctgtatcacc
      781 gcttccgttg ggaagtggaa tgcagtatga gagctcggtt tctcttggat acttaaatca
      841 atcatttcaa aatgcagtta tggaagggat acgctatggt tgcgaacaag gattatatgg
      901 ttggaatgtg acggattgta aaatctgttt taagtatggc tt
//
LOCUS       DACDIG010000219          935 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.227, whole genome shotgun sequence.
ACCESSION   DACDIG010000219 DACDIG010000000
VERSION     DACDIG010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..935
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.227"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..818
                     /locus_tag="IV247_002988"
     CDS             <1..818
                     /locus_tag="IV247_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP8778971.1"
                     /translation="TRYQLIKQLIRTKQHFLENLTYKCNTLAREMRDESTSLFSATLI
                     SLMTEDFTLDELAELPLEAFCDLLQEKGKGRFKQPEKIAKAIQRAITMSYRLGALAQE
                     SINVVLSVLVREIRALEKNIKELDKAIEQVIVVIPEYQCLTSIPGVGKVYAAGLIAEI
                     GQIERFEDQTKLAKYAGLSWKVNQSGNYQSQNTPLTKQGNRYFRYYLVEAANSVKNYL
                     PEYKAFYQSKYKEVPKHQHKRALVLTARKFTRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          334 a          182 c          163 g          256 t
ORIGIN      
        1 gtactcgcta tcaacttatt aagcaattga ttcgtacaaa gcaacatttt cttgaaaatc
       61 taacgtacaa atgtaatacg cttgctcgag aaatgcgaga cgaatcgacg agtcttttta
      121 gtgcaaccct catttcactc atgacggaag atttcacatt ggatgaactt gctgagcttc
      181 ctttagaagc tttctgtgat ttacttcaag aaaaaggaaa aggccgtttc aagcaaccag
      241 aaaaaatcgc taaagcaatt caacgtgcaa ttaccatgag ttaccgccta ggtgctcttg
      301 cacaagaatc aattaatgtt gttttaagtg ttctcgtacg agaaatacga gcacttgaaa
      361 aaaatatcaa agaattagat aaagccatcg aacaagtaat tgttgtcatt ccagaatatc
      421 aatgcttaac cagtattcca ggcgttggta aagtctatgc tgccggttta atcgctgaaa
      481 ttggtcaaat cgaaagattt gaagatcaga caaaattggc aaaatacgct ggattaagtt
      541 ggaaagtgaa ccaatctgga aactatcagt cgcaaaatac acctctgaca aaacaaggaa
      601 atcgatattt tagatactac ttagttgaag ctgccaactc tgtaaaaaat tatcttcctg
      661 aatacaaagc tttttatcaa agtaaatata aggaggttcc aaagcatcaa cacaaacgtg
      721 cactcgtcct aaccgcaaga aaatttacgc gtctggtgga tacgctacta cgtaaacacc
      781 aactctatac gccaccaagg agtgtgatag agaaataaca atccgttatt gacgcaaatc
      841 cttgaatcaa cccgaaaaaa atttgatttt gatcgggtct agtttcgtgc gcttcaaaac
      901 atattactca ataaagaaaa tcttaaattt catct
//
LOCUS       DACDIG010000220          932 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.228, whole genome shotgun sequence.
ACCESSION   DACDIG010000220 DACDIG010000000
VERSION     DACDIG010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.228"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(1..347)
                     /locus_tag="IV247_002989"
                     /pseudo
     CDS             complement(1..347)
                     /locus_tag="IV247_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            423..578
                     /locus_tag="IV247_002990"
                     /pseudo
     CDS             423..578
                     /locus_tag="IV247_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
     gene            597..>932
                     /locus_tag="IV247_002991"
     CDS             597..>932
                     /locus_tag="IV247_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8778972.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDE"
BASE COUNT          319 a          119 c          172 g          322 t
ORIGIN      
        1 aatatttaga attttgcaca tcgctgatat ggaatattta tgcttgttgg catcaattac
       61 ttgtcttttc gtccgaatat cagcgccgct tgctttaaaa tatcattctc catttcgagt
      121 tgcttatttt tctttcttag tgcgatcaat tctgcttgtt ctggtgttaa gttgtctctt
      181 tctttgaatg agcccgttga ttgtgcttgc ttcatccatt tatcgaaaga agaaggcgta
      241 agctcatact ctcgaataat ttctgcgcga ggcttaccag cgagatagag atcgacgatt
      301 tgttgtttga attcttttga ataggttctt cgttgacgtc ttggcatgaa aaatcctcca
      361 gtgtgttttt tattattcta cacaccttat tttttctgtc tagtctagtg tagccgattc
      421 aggataagga taagtgtatt aattgtggaa aatgtaaaaa agtttgccaa atgaataaag
      481 attcagttga gaattgcaat catctggagt gtatcagatg tggacgatgt aaaaatgctt
      541 gtccagtgga tgcaattagt tatggagtaa gaaaatagag aggagtagtt aggaaagtga
      601 taaaaaatgc tatagcatat attacaagaa agaaaaatag aactttaata atttttatca
      661 tattaactat agttctttct tgcttgtatt cttgtttaac aataatgaaa tcaagcaatg
      721 aaatagaaaa ggctttatat gaaagttcta attcttcaat atcaataaca aaaaaagatg
      781 gtaaatattt taatgttaat caatttaaag atattgaaaa attaaaagaa attgaagaaa
      841 aaataattca atatgatgga ttagcaaaac taaaagatgc taaagtagtt agcggagaac
      901 aaagaataaa tagagaagat ttatctgacg aa
//
LOCUS       DACDIG010000221          926 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.229, whole genome shotgun sequence.
ACCESSION   DACDIG010000221 DACDIG010000000
VERSION     DACDIG010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.229"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..76
                     /locus_tag="IV247_002992"
     CDS             <1..76
                     /locus_tag="IV247_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP8778973.1"
                     /translation="LKNCTLNQLQALGFARKFLQIKVV"
     gene            199..837
                     /locus_tag="IV247_002993"
     CDS             199..837
                     /locus_tag="IV247_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP8778974.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT          307 a          145 c          141 g          333 t
ORIGIN      
        1 tctaaaaaat tgtacgctta atcagttgca agcacttggt tttgctagaa aatttttaca
       61 aataaaagta gtataatcaa aatgaatata taagataaat ttttagcaat acaaatatcc
      121 ataaaaaaac attgcctatt ttaactcttc atgtatactg actctatctt caaaaactga
      181 atagtaggag ttattatcat gaataacatt ttaggaaaaa aaatagaact taaaaaaggg
      241 atattgggca ctattgtcgt tgtactcggt ttgttagggt tgctatttgc gccatccatt
      301 attagcattt tttctttaac tatcactgga tttatagcat ttacacttgt ttttggtaaa
      361 aaagatacga aaaaaatgtt ctcgaaacct gtcgctcctg tcaaaaatat agccaaatat
      421 ttttttatca atgtagtaat cagtgccgca gtctcggtat ttttgcaaca aattttgaaa
      481 tggggtttaa caggcaatcc aataaatgag gctttcagcc cgttattatt tattattttg
      541 cctatcatga tacttggaga agaattgttc tcagtgtatt ttctcgctat tttttctagt
      601 aagttcagca ttcctgtcgc tagtttcttg tcagcaatca tctttggatt tatccattac
      661 agtacttatg ataatggaaa tattttacac actgtagcac acatcctttt gatacaaggg
      721 gtggcaagat tgttattcaa ccaagctgcc attaaaagca actcgatcat tacaagttgg
      781 gcagttcatg tgatatttga tttcacagct atattgctag tcgttttatt ctcctaaatt
      841 tttatagaaa taatacatgc tctattgcta agaaagcata ctttatttcc tttaaacaaa
      901 aagacagatg tatcaatgga ttaaaa
//
LOCUS       DACDIG010000222          918 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.230, whole genome shotgun sequence.
ACCESSION   DACDIG010000222 DACDIG010000000
VERSION     DACDIG010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.230"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            25..>918
                     /locus_tag="IV247_002994"
     CDS             25..>918
                     /locus_tag="IV247_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAP8778975.1"
                     /translation="MLMVQFTGIAMSVITINPQVIHADGIAIYDAQFNCTTEGSGSGT
                     DSDGNDTGSGAVTGDVAKNIEDIYKYMNGEYGLSGASIAGILGNWKQESGIDPRAVEG
                     YYGDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVDWSKKNNGGSWWTTEAQLDFMF
                     KGDGGFVGILKDYALNASDDVVENAVTFHKNWEISADSESAVIAVRGENAKEIWKYMK
                     SKGMDGAKDESKINKISGGSSTGDGSNNSSSSASGTVEDACATTDDANSGSGSIGKEG
                     KINGKKGQVIANMTYEEALKKY"
BASE COUNT          325 a          120 c          233 g          240 t
ORIGIN      
        1 aagagaaaga ttgccacatt gcttatgtta atggtgcaat ttacagggat tgcaatgagt
       61 gtaatcacaa taaatccgca agtgatacat gctgatggga tagctattta tgatgcacaa
      121 tttaactgta ctactgaagg tagtgggtca ggaacagata gcgatggaaa cgatactgga
      181 agtggcgctg taacaggtga tgtagccaaa aatattgaag atatttacaa atacatgaat
      241 ggagaatatg gcttatcagg tgctagtatt gcaggtattt taggtaactg gaaacaagaa
      301 tcaggtattg atcctcgagc agtagaaggg tattacggag atggacagag agaagttgac
      361 ttcatcaaac aatttactga tggtctgatg ggtattggct atggtcaatg gacagctggg
      421 cgacatacag cattagttga ttggtctaag aaaaataatg gcggttcttg gtggactaca
      481 gaagcccaat tggactttat gttcaaaggt gatgggggat tcgtaggaat attaaaagat
      541 tatgctttaa atgcttctga tgatgtcgta gaaaatgcag taacttttca taaaaactgg
      601 gaaatttcag ctgattccga atcagctgta atagctgtac gtggtgaaaa tgcaaaagaa
      661 atctggaagt acatgaaaag caagggtatg gacggtgcaa aagatgaaag taagatcaat
      721 aaaatatccg gaggttcaag taccggagat ggatcgaata attcatcaag ttcagcatca
      781 ggaacagtag aagatgcctg tgcaactacg gatgacgcta attctggttc tggatcaatt
      841 gggaaagaag gaaaaattaa cggtaaaaaa ggtcaagtaa tagcgaatat gacttatgaa
      901 gaagctttga aaaaatat
//
LOCUS       DACDIG010000223          914 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.231, whole genome shotgun sequence.
ACCESSION   DACDIG010000223 DACDIG010000000
VERSION     DACDIG010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..914
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.231"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(354..848)
                     /locus_tag="IV247_002995"
     CDS             complement(354..848)
                     /locus_tag="IV247_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778976.1"
                     /translation="MNKLPEKNRVFRLKGDLSQFKIDENGQFFVWMRHEHCWCNPFVL
                     KKENEIRTKIANGEWDYLFRWEDESFIANDMDHAFVSFLDNTWGTWYDCYLEKLGFQN
                     IDIDAIECLKEVDQKYEEEPYYLLQQKLANNSVFQVEIWENEDGTYEGLAQEIPFSDR
                     KSSI"
BASE COUNT          271 a          203 c          102 g          338 t
ORIGIN      
        1 tacactccaa tagttggata ctcgactact aaaaattggt ctggatcaaa ctgttctttt
       61 ttcaacattt ctcgtatttg aaacaaatga tccttctcta agcaatcagg taattccttt
      121 attctttgcg ttactaatga cccctctatc gctttccatt catttaaata ttgtttcata
      181 acaattcatt ccttttatgt taactatttt tcctaggaaa ttgttttaaa acgccactct
      241 cccagaaaac gtaagatttg gttagctgga atataccttc cagctaacca caattttcca
      301 ttactatatt gtaagcttgc tttcacttca taaaaattgc tgatatactc ttttcaaatc
      361 gaactcttcc tatctgagaa gggtatttct tgcgctagcc cctcataagt tccatcttca
      421 ttttcccaaa tttctacctg aaacactgaa ttattggcta atttttgttg caagagataa
      481 tagggttctt cttcatactt ttgatcgact tctttaagac attcaatagc atcaatatca
      541 atattttgaa agcccaattt ttctaggtaa caatcgtacc aagtacccca tgtattatcc
      601 agaaatgaga caaatgcatg atccatatca tttgctataa aagattcatc ttcccatctg
      661 aagaggtaat cccactcacc atttgcaatt ttagttctga tttcattttc tttttttaac
      721 acaaaaggat tacaccaaca atgctcatgt cgcatccaaa caaaaaattg tccattctca
      781 tctattttaa attgactgag atcacctttc aatctaaata cacggttctt ttctggtaac
      841 ttattcatgc ttcaaccacc tcatactgtt ctctcatacc tttgacttca ttaaaaaaaa
      901 ctctcgcttt ttct
//
LOCUS       DACDIG010000224          886 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.232, whole genome shotgun sequence.
ACCESSION   DACDIG010000224 DACDIG010000000
VERSION     DACDIG010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.232"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..309
                     /locus_tag="IV247_002996"
     CDS             <1..309
                     /locus_tag="IV247_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778977.1"
                     /translation="NAKDGIVVENTSNSKETGTTTKKDKSAEITTKNYLIKTVYFDER
                     KTAILKDEEGSELKLPITVSDKFNGTFSLQTAEGSPDNLKVGQTIILGKNDELYSFVK
                     "
     gene            complement(326..>886)
                     /locus_tag="IV247_002997"
     CDS             complement(326..>886)
                     /locus_tag="IV247_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778978.1"
                     /translation="ENKGTWDFWATLANKELSDKSDTGIRNFFESNRDDYMYANNQNK
                     LNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISELGGQYEPLVEAVREE
                     LRLNKFPDVQNIDVSQIEINVKITNKLDYGSWADIALEKYIYSTLKEFIENRMDIMYL
                     QHPQYLNFGVEIATPLEEWKMEQGLD"
BASE COUNT          289 a          170 c          128 g          299 t
ORIGIN      
        1 aatgccaaag atggaatagt cgtagaaaat acctctaatt ctaaagaaac tggtactact
       61 acaaaaaaag acaaatctgc tgagatcact acgaaaaatt atttaataaa gactgtttat
      121 tttgatgaac gaaaaacagc aattctcaaa gatgaggaag gatcagaatt aaagctacct
      181 ataacggtat ccgataagtt caatggaacg ttctccttac aaaccgctga aggatcacct
      241 gacaatctca aagttgggca aacaatcatt cttggtaaaa acgacgaact gtattcattt
      301 gtaaaataaa aaaatggagc aaaggctagt caagcccttg ctccattttc cattcttcaa
      361 gaggtgtagc tatttcaaca ccaaagttta aatactgtgg atgctgtaga tacatgatat
      421 ccattctatt ttcgataaac tcttttaacg ttgagtagat gtatttttct aatgctatat
      481 ctgcccaact accataatca agtttattcg tgatcttgac gtttatttca atttgagaaa
      541 catcaatatt ttgaacatca ggaaacttat tcagtcttag ctcttctcta accgcttcta
      601 ctagcggttc atattgtccg cctagttcgc taattgttga aactatttca tctgaattcc
      661 aacgatcttc caataccttt gctacatttt ctttaggcgg ttgtctttct ataaattctt
      721 caggtgtttc atcaaactct actctaaagt tcaatttatt ttgattgtta gcatacatat
      781 aatcatctct gtttgattca aaaaagttac gaattcctgt gtctgattta tcacttaact
      841 ccttatttgc taaagtagcc caaaaatccc aagttccttt attttc
//
LOCUS       DACDIG010000225          880 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.233, whole genome shotgun sequence.
ACCESSION   DACDIG010000225 DACDIG010000000
VERSION     DACDIG010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..880
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.233"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            147..848
                     /locus_tag="IV247_002998"
     CDS             147..848
                     /locus_tag="IV247_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP8778979.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT          318 a          154 c          146 g          262 t
ORIGIN      
        1 caataccatt gtatattttt tacataataa caaattatat ttgtaaagta ttaaaacttg
       61 tttctaatgt tggtatgtta aaactttaca aattctttaa aaaaagtgac aataaatata
      121 gtgaacaata aggagaggac tttttaatgc gtgttaataa aatcgatttt cacttttcaa
      181 aactcagata tcaacctgat tttgaacatt tctcggatga agagttccaa cagatcaagg
      241 aacatactgt tcttcgttct tataaaaaag gacagatcct tttcgacgat ggtgatccca
      301 gacaacggtt gtatatcttg accaaaggac tcgttcgttt agaacgttat gatgaaagtg
      361 caacttatta ctactatgac tatgtgaata caaataacct ttttcctatg tcaggaatgt
      421 ttggagaaga gacctacact tatacagctc aagcgatgac tgatattgaa acattctacc
      481 ttcctgtttc ctactatgaa aaattagcac gaaaaaatcc cgaacagttg atctatatga
      541 tacaaaaaat ttctaaagta gttgaaatac atgaattgcg tatccaaatc ggattgacct
      601 ctagcgcctt tgaccgggta aaacaaaatt tattcatttt gaaagaagaa ttgggcgtac
      661 caacaaaaga tggtcatatc accattcctt atccgatcac cttaaaagaa ttagcagtga
      721 atagcggcac gacacgtgaa actgctggac aagtaatcaa acaactacgt gattccgggc
      781 tgttagatta tgacaaaaag cgttttactt tctgtaagac agctactact ccagctatga
      841 gagaataacg cgaacaaaaa taagcagtga ccctgataaa
//
LOCUS       DACDIG010000226          875 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.234, whole genome shotgun sequence.
ACCESSION   DACDIG010000226 DACDIG010000000
VERSION     DACDIG010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.234"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(28..>875)
                     /locus_tag="IV247_002999"
     CDS             complement(28..>875)
                     /locus_tag="IV247_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAP8778980.1"
                     /translation="ISVLISKITKTIRVFYMMNFIVQIFYITLSSSRGAQVVICSFLV
                     LFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFSFGTNYAKDFLATIPVSIDRK
                     VEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIWKAALKVIRPTLLFGYGETDF
                     YGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLVSSGIIAFICIYAFYVLNIFS
                     LLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFNKRDVISIVFWYYLGSISYLY
                     LKKEN"
BASE COUNT          336 a          151 c          104 g          284 t
ORIGIN      
        1 gtccaagcaa tgaaagctgt cttcatatta attctccttt tttaaataca agtaagaaat
       61 gcttcccaaa taataccaga aaacaattga aatgacatcg cgcttattga aaagtacatg
      121 agcctccacc atttcatcta taaaaataga tagtataaaa attaaaacaa taccaagaaa
      181 cgcataattc actttacttc taaaaaatgt tttgagtaaa ctaaagatat ttaacacata
      241 gaaagcatag atacatataa aagcaattat accacttgat acaagtactt gaagaaatcc
      301 attatgcatg tttccatgtg ctctttttag ttcattcaca tctgtagtat caagatctac
      361 ttttttcaaa aattttgcat cgctattccc atagaaatcg gtctctccat aaccaaataa
      421 caaagttggt cttataactt taagcgcagc tttccaaatc gtaaaacgac cagctgaaat
      481 ttcggagtta tcactagaat gctgtaatat tacttctttt tctggaagtg gagttttatc
      541 tgattcattt gactctataa cttctacttt tctatcaatc gatacaggaa ttgttgctaa
      601 aaaatcctta gcataattag taccaaaaga aaaatatacc aatagaacta ataatatgct
      661 taccatccat ttgattttta caggtagtac ttctctaatg ctctttctaa attttgaatt
      721 ggcaaacaac aaaagaaata atactaaaaa agaacaaatt actacctggg cacctctaga
      781 cgaagaaagt gtaatataaa atatttgtac gataaaattc atcatataaa aaacacgaat
      841 cgtttttgtt atttttgaaa ttaatacact aatta
//
LOCUS       DACDIG010000227          864 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.235, whole genome shotgun sequence.
ACCESSION   DACDIG010000227 DACDIG010000000
VERSION     DACDIG010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.235"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(7..243)
                     /locus_tag="IV247_003000"
     CDS             complement(7..243)
                     /locus_tag="IV247_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778981.1"
                     /translation="MEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTSLEC
                     VWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            complement(259..543)
                     /locus_tag="IV247_003001"
     CDS             complement(259..543)
                     /locus_tag="IV247_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778982.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            complement(646..828)
                     /locus_tag="IV247_003002"
     CDS             complement(646..828)
                     /locus_tag="IV247_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HAP8778983.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKAIGLAVSS
                     FLTLLTLFVTLSFSFV"
BASE COUNT          285 a          166 c          146 g          267 t
ORIGIN      
        1 cttatattaa gaaaaatgga tatttccaat tgcatcccac tcggcaccat tgtggtataa
       61 gacgtgaccg agcgttacgc ctttgaaatg ctcttctaaa tacatccaaa cgcattcaag
      121 gctagtacct gctttgagat tgtagaagtc tgcatccaat accttttcag aagttatcgg
      181 atcaatgcga gttggtacct caataaattc agtccaaatt tctgcaaaag tcttaccttc
      241 caaagttcac actccctatc aattaattcc aaaagcaaat agagaaatca agcgaccgtc
      301 aatagtcact tcaacaactg aaaatgccgt tgaaacagtt ttaacatcta cactttccaa
      361 gttcccgatg aaggaatctt tgtgctttag aatagctttc tttagctgtc tgttatacgc
      421 ttgccgactg ttaaactttt tgaattctcg atccttgatc agctgtttaa cgatccctgc
      481 attaattgta taactttctg caatagcaac gtgagtaggt tgctgtaaag tagctgttag
      541 catcttgtca ctccttccta aaagttaatt agcttgttta caaatacatt atacacaaca
      601 gtgtgtataa tgtaaagaga aaaaggtgct aatggatcag ctgttctaaa caaaagaaaa
      661 cgataaagta acaaataaag tcagcaaagt taaaaaggat gaaacggcta gtccgattgc
      721 tttgcttgtg tgattttcgg ctttcttata gatcaaatag gaaattatca gtgatattcc
      781 agtaaagaat aatgtactag ctgtcactaa aagaaatagg ttaatcatac gatacactcc
      841 ttctgtaata ttggtatcag atac
//
LOCUS       DACDIG010000228          857 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.236, whole genome shotgun sequence.
ACCESSION   DACDIG010000228 DACDIG010000000
VERSION     DACDIG010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.236"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..807)
                     /locus_tag="IV247_003003"
     CDS             complement(<1..807)
                     /locus_tag="IV247_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778984.1"
                     /translation="MLLTLLGYLCLILAFFGAIAIIIGQFQTNNPIMRNIGIGIVIVT
                     FIVFNATKIFGSNDKQFGISGVNNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDGK
                     KASDDVLATIKPSQSQFEVFYTVKENSPVHKLTLVFNVDGEEYYKDVKINKTSTKKII
                     ESAKKEVATEESTTESSSTVEAELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQMINVTLFNSVESMDQDKKVTVARELGKRVIGLYRGTAKPDSD"
BASE COUNT          255 a          168 c          116 g          318 t
ORIGIN      
        1 gtctgaatct ggtttagcag taccacgata caaaccaatt actctttttc ctagttctct
       61 tgcaaccgta acttttttat cctgatccat agattcaact gaattgaata aggtaacatt
      121 aatcatttgc ttttcagtat ccaatttaac agttttgata gctttgaaga cttcagtgtt
      181 aaaccgatca gccatagaac cagaaacaga ttcaaacgat ttgacttttt caggatcaaa
      241 atcaatttta tctggtagtt ccgcttcgac agtagaactt gattcagttg tactttcttc
      301 tgtagctact tcttttttcg ctgattcgat aatctttttc gttgatgttt tatttatttt
      361 tacatcttta tagtattctt ctccatcaac gttaaaaacc aatgtcagct tgtgtacagg
      421 gctattctct ttaactgtat aaaacacttc aaattgtgat tgagacggct taatagtagc
      481 gagaacgtcg tctgatgctt tcttgccatc ttccttcact gtcagcgaaa catttttata
      541 actcgttaca ttttcaaatg ttccatctat tactaccctt cccaaagaat ctggagcata
      601 actattatta actcccgata taccaaattg tttatcgtta cttccaaaaa tcttagtggc
      661 attaaacaca ataaaggtta caatcacaat tccaatgcct atgttacgca taatcggatt
      721 atttgtttga aattgaccaa taattatagc gattgcccca aaaaaagcaa gaattaaaca
      781 taaataacct aacagggtta atagcatgat tcatcacaac tttctattct ttatttttct
      841 ttttggtttt cgtttgt
//
LOCUS       DACDIG010000229          813 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.237, whole genome shotgun sequence.
ACCESSION   DACDIG010000229 DACDIG010000000
VERSION     DACDIG010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..813
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.237"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            22..777
                     /locus_tag="IV247_003004"
     CDS             22..777
                     /locus_tag="IV247_003004"
                     /inference="COORDINATES: protein motif:HMM:NF012744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP8778985.1"
                     /translation="MKSQFEKYGISTLKYDALLKKNNFEINSADFFIGASSITERSLL
                     NMGIEKKQIKIFPYGVNIVKENDKLMNLRTRKKRLKFLFVGRVTLEKGIGVLDSFFEK
                     FGIEDAWTFAGDYSDSKKFYEKYEDKVQFVGHVSKIKMFELYRDNDVLVFPSLADGFG
                     LAVIEALSNGMYVICSDSTGAKDIIMEGKNGDVFLTGDENDLNNKIQYYKKNINKIDR
                     LKIKETVNDLTWERYNKNIIKSLEYFTVGMNNE"
BASE COUNT          328 a           69 c          143 g          273 t
ORIGIN      
        1 tctgcaccta gtgttgtttt tatgaaaagt caatttgaaa aatatggaat atcaacattg
       61 aaatatgacg cactcttaaa aaagaataat ttcgaaatca atagtgcaga ttttttcatt
      121 ggggcttctt caataacaga aagatcctta ttaaatatgg gaattgaaaa aaaacaaata
      181 aaaattttcc cttatggagt gaatatagtt aaggaaaatg ataaattaat gaatctaaga
      241 acaagaaaaa aaagattaaa atttttattt gtgggaagag taaccttaga aaaaggaatt
      301 ggggttttag atagtttttt tgaaaaattt ggaattgagg atgcatggac atttgctggc
      361 gattattcag attctaaaaa attttatgaa aaatatgaag ataaggtaca atttgtggga
      421 catgtatcaa aaataaaaat gtttgaactg tacagggata atgatgtttt agtatttcct
      481 tctttagcgg atggttttgg tttagctgtt attgaagcat taagcaatgg aatgtatgtt
      541 atatgttcag attctacagg tgctaaagat attattatgg aaggcaaaaa tggagatgtg
      601 tttcttacag gagatgaaaa tgatttaaat aataaaatac aatattataa gaaaaatata
      661 aataagattg atcgtttaaa aattaaagaa actgtaaatg atttaacatg ggaacgttat
      721 aacaaaaaca ttataaaatc attagaatat tttaccgtag gtatgaacaa tgaataaatt
      781 attaatgatg ttaaactttt tttatctcta cgt
//
LOCUS       DACDIG010000230          791 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.238, whole genome shotgun sequence.
ACCESSION   DACDIG010000230 DACDIG010000000
VERSION     DACDIG010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.238"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..205
                     /locus_tag="IV247_003005"
     CDS             <1..205
                     /locus_tag="IV247_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP8778986.1"
                     /translation="TLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFFENV
                     VTGKENWNDLYHANEVLKIAEEK"
     gene            206..>791
                     /locus_tag="IV247_003006"
     CDS             206..>791
                     /locus_tag="IV247_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAP8778987.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKK"
BASE COUNT          309 a           94 c          138 g          250 t
ORIGIN      
        1 tactttagat gaatgtatca atgttgactt attaaataat aaaataagtt ttgaagaaaa
       61 cattgttaaa gaaatgcctg aatctgctaa tgatatgtat gagaaggagt tgaaatattt
      121 ctttgaaaac gtagtaacag gcaaagaaaa ctggaatgat ttatatcatg caaacgaagt
      181 attgaaaata gctgaggaga aataaatgga aattttattt actatttgtg gtagagctgg
      241 gtctaagggg tttaaaaata aaaatttgaa atatttggca ggaaagcccc tagtccattt
      301 cgctttagca atgatagata tctatcaaca aaaacatcct gaacataatt gcacgacttg
      361 tttgaatacg gacagtaatg agttagtaga attgacgact aagttaaatc cgtctgtgaa
      421 aattgtagaa cgtaaacctg aattagctac agatactgta gggaaaattg aagtgatacg
      481 tgattcattc ttacaactag aaagagaaga taatatattt gatttaatta tagatttaga
      541 tttgacttca cctttacgtg aagtttctaa tcttgaagaa ttaatagatg aacatctaaa
      601 aaatttagat aaagacgtct acttttcagt tgtttctgca agacgtaatc cctattttaa
      661 tatggtaaaa agaattgaaa atgaagttac tatagttaat aaatcaagat acacatctag
      721 gcaacagaca cctgaagttt ttgaaatgaa tgcctcaatg tattctttta aacctgaatt
      781 tctaaaaaaa a
//
LOCUS       DACDIG010000231          770 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.239, whole genome shotgun sequence.
ACCESSION   DACDIG010000231 DACDIG010000000
VERSION     DACDIG010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.239"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..505
                     /locus_tag="IV247_003007"
     CDS             <1..505
                     /locus_tag="IV247_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778988.1"
                     /translation="IQSIFGIEEVFVGDKCIFFDQPLLSDGYGIDETDIFAKVNSIFS
                     GKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTLLISPISTISFSPSMM
                     FEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLSEEKILLPENWAELRK
                     LVEYGII"
     gene            492..>770
                     /locus_tag="IV247_003008"
     CDS             492..>770
                     /locus_tag="IV247_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP8778989.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLA"
BASE COUNT          264 a          108 c          116 g          282 t
ORIGIN      
        1 catacaatca atctttggga tagaagaagt ttttgtcgga gataaatgta tcttttttga
       61 tcaaccttta ttatctgatg gttatggcat agatgaaaca gatattttcg ctaaagtaaa
      121 tagtatattt tctggaaaaa aagaaacttt aattaagctt catcctagag caaggacaaa
      181 tcgttatggt gaaaaaaaca tattgaattc cagtttaccg tttgaagtct tgctacttga
      241 aaatgacttc tcttcaacac ttttgattag tcctatatct acaatttctt tttcaccatc
      301 tatgatgttt gaaaagacga tcaactctat tatgttggct aaattaattt taaatgaata
      361 tacagcatta tctgaagata aaaaagaaac attgacaaat attgttcttt tctgtgaaca
      421 ttataaccgt ctatcagaag aaaaaatttt actcccagaa aactgggcag aattaagaaa
      481 gttggtagag tatgggatta tttaacacac tttccaagtc aaaattagtt aagtccagtt
      541 tctggtatac tatagggaat ttttttatta agggaatttc attcataact attcctattt
      601 ttgtaaatat tactactcaa gcagaatatg gtttgattaa taatttcact tcatttgtat
      661 caattttttc gattcttgtt gggctaagcc taaattcggc aattaataat gcaagttttg
      721 actataaaga tgacataaat gaatttatgt catcggtact ttctctagct
//
LOCUS       DACDIG010000232          758 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.240, whole genome shotgun sequence.
ACCESSION   DACDIG010000232 DACDIG010000000
VERSION     DACDIG010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..758
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.240"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>758
                     /locus_tag="IV247_003009"
     CDS             <1..>758
                     /locus_tag="IV247_003009"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778990.1"
                     /translation="KELGNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNA
                     ETKAKKSGTEKEVDRIIVLKDTLRTALDSFSRMFNQRTTLKEIGKSDKLQFYYNLGDL
                     RAETNVMEAQFLNTFDFITSQAKKDDIVMIHGFDKLSIETVGLIKKRLTMLEEDGIRL
                     AFLFDTIGSGEDNEYKEDIETADIFNTDGKIYQSFESDFNYTIIGKMTSGELMKYEKK
                     VAPARKGEDDQKLSVDLRQYLTTGRSNKFQIRRP"
BASE COUNT          300 a           97 c          143 g          218 t
ORIGIN      
        1 aaaaagaatt aggtaacttt tatataagtc agaaactttg ggatattaat tatgaagaac
       61 gttcaaaact tttggtgaac agtagtgata caatgccagt tcttggggat tttattagct
      121 atttgactaa tgctgaaaca aaagccaaaa aatctggtac tgaaaaagaa gtcgatcgta
      181 ttattgtatt aaaagacaca ttgagaacag cattagacag cttctctagg atgtttaatc
      241 aaagaacgac actgaaagaa atcggtaaaa gtgacaaatt acagttttac tataacttag
      301 gtgatctaag agcagaaacc aatgttatgg aagctcaatt tttaaatacc tttgatttca
      361 tcacatcaca agcaaaaaaa gatgatattg ttatgattca cggcttcgat aagttatcta
      421 ttgaaaccgt tgggctaatt aaaaaaagat taacaatgct tgaagaagat ggaattcgtc
      481 ttgcctttct atttgataca atcggttcag gagaagacaa tgaatacaaa gaagatattg
      541 aaacagctga tatattcaat acagatggaa aaatttacca aagttttgaa agtgatttta
      601 attacactat cattggaaag atgaccagtg gagagttaat gaagtatgaa aaaaaagttg
      661 ctcctgctag aaaaggagaa gatgatcaaa aactttctgt tgatttgaga cagtatttga
      721 caacaggaag aagtaataaa tttcagatca ggagacca
//
LOCUS       DACDIG010000233          756 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.241, whole genome shotgun sequence.
ACCESSION   DACDIG010000233 DACDIG010000000
VERSION     DACDIG010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.241"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>756
                     /locus_tag="IV247_003010"
     CDS             <1..>756
                     /locus_tag="IV247_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP8778991.1"
                     /translation="VISYIQSYFTINFNHFKYLILSFISTFLNIAISVALMQTLYFDD
                     KYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYALKISLPLIPHSLANIVLA
                     QFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWVPWFYGEMSKNNEKKVKK
                     VSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVPITLGYFFQFLYSLPVNA
                     EFYEKKTSFIAIGTVASAIVNILLNMIFIPRLG"
BASE COUNT          235 a           94 c          134 g          293 t
ORIGIN      
        1 tgtaattagt tatatccagt cttattttac gattaacttt aaccatttta aatatttgat
       61 tttatctttt atatctactt ttttgaatat tgcaatctct gtagctttga tgcagacatt
      121 atattttgat gataaatatg ttggcagagt agtcggcgga acactgtcat ttgtgttgat
      181 aggtttagta atttatttaa gaataatgat aaagggtgct catttttttg ataaaaaaca
      241 gtggagatac gctttaaaaa tctctttgcc gctaatccca cattccttag caaatatagt
      301 tttagcacaa ttcgatagaa ttatgattaa ttcttattta ggtcctgctg cggggggaat
      361 atattcctac attagtaaca taggtattat tctaagcgta atatggatgt ctactaataa
      421 cgcctgggtt ccatggttct atggtgagat gagtaaaaat aatgaaaaaa aagtgaagaa
      481 agttagtaac tattatttag tagtctttac tttgattacg atgattgtaa tggtagtttc
      541 gattgattta gcaaaattta tggctccgaa agactattat tctgggttat ctttagtggt
      601 tccgataacc ttaggctatt tctttcagtt tttatatagt ttaccagtta atgctgaatt
      661 ttatgagaaa aaaacatcgt ttattgcgat tggtacagta gcaagtgcta tagttaatat
      721 actattaaac atgattttca tacctaggtt aggaat
//
LOCUS       DACDIG010000234          754 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.242, whole genome shotgun sequence.
ACCESSION   DACDIG010000234 DACDIG010000000
VERSION     DACDIG010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.242"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(91..>754)
                     /locus_tag="IV247_003011"
     CDS             complement(91..>754)
                     /locus_tag="IV247_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HAP8778992.1"
                     /translation="RRIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQE
                     QPLEAIVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSL
                     TDVKLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQ
                     YQFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGE
                     AA"
BASE COUNT          204 a          177 c          145 g          228 t
ORIGIN      
        1 ttgtgaatgc tgcggtgtta tggctacgat tgcccttgac aatgagcatc ctcggatctg
       61 attttctccg gcagaagcac aatcattcaa ttacgctgcc tctccagagg tatcagatcc
      121 gcgctcatta tcaaggattc gcttcgccaa tctctgttgt cgaatttgga tcggtgtttc
      181 atacgccaac gtgccatgta agcgtagata gttccaccaa tgcacataat caaacaattc
      241 tagacgtagc tgtgccagtg tctcaaattg gtattgatgc acgaattcta ctttgacaga
      301 tttataggtt gattccacaa cggcattatc atacggacaa cccttcctac tcaatgaacg
      361 agtaattcca aaaccattca gaatctcatg aatggtttgg ttatcaaatt cctttccccg
      421 gtctgtatga aaaagcttga cgtctgttaa agaatacggt atccgtccaa aggcttcttt
      481 taccaatgag gcatctttct tctcaccaca ggaataacca ataatttctc gattaaacaa
      541 atcaagtatt aagcagatat aatgccactt tttccccacg cgaacataag taagatccgt
      601 aacgatggct tccaatggct gttcttgtgt aaaggtccga tctaatacat tcgctgtttt
      661 cgcttcatta caagctgttc gttgcccttt aaaatgagcg atcgtatagg tagatgtcaa
      721 tccgcggcgt ttcatgattc gaccgattcg gcgc
//
LOCUS       DACDIG010000235          751 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.243, whole genome shotgun sequence.
ACCESSION   DACDIG010000235 DACDIG010000000
VERSION     DACDIG010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.243"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(593..736)
                     /locus_tag="IV247_003012"
     CDS             complement(593..736)
                     /locus_tag="IV247_003012"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778993.1"
                     /translation="MEKEAFDLHVLGTPPAFVLSQDQTLIKSSNNLAIVKLNLFASIL
                     LAC"
     gene            655..>751
                     /locus_tag="IV247_003013"
     rRNA            655..>751
                     /locus_tag="IV247_003013"
                     /product="16S ribosomal RNA"
BASE COUNT          307 a          123 c          131 g          190 t
ORIGIN      
        1 ccttaggcag cttttgctgt gtagaaagga ataaataata acgaaagttc aaaagctaat
       61 gccgactatt ctagacatta aagaagacga aaacgaacga taaagaaaga aaagttatga
      121 ataatcgttt attgaaaaaa acttgaaaaa atacaaaaaa ttcgttgacc aagctaaatt
      181 tagttggtat attattactt gtccaaaaga cagaagaaaa tatttattca aaaacataac
      241 aaaaaagtta ttgacatgtt actaacgaca tgataagata actgagtcgt taaaaaagaa
      301 ccacttgcga agcttgattc tatcgaaaaa aacttcaaaa aagttcttga caaataacaa
      361 acgataagtt atgataaatg agttgccgaa agatttcgaa aaacgaaatc gaaaaaaact
      421 tttaaaaaag agttgacaat cgcttgtctc tctgatatga tataaaagtt gctacggcag
      481 ccaaaaatga atcgggaaac actctcgatg aagtagacct ttgaaaactg aacaaagtaa
      541 gacaaaccaa atgtgtaggg cgtcttgatt caattcaaga caacaaacat ttttaacaag
      601 caagcaatat gctagcaaac aaattgagct taacaatcgc aagattgttc gaacttttta
      661 tgagagtttg atcctggctc aggacgaacg ctggcggcgt gcctaataca tgcaagtcga
      721 acgcttcttt ttccaccgga gcttgctcca c
//
LOCUS       DACDIG010000236          723 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.244, whole genome shotgun sequence.
ACCESSION   DACDIG010000236 DACDIG010000000
VERSION     DACDIG010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.244"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            78..193
                     /gene="rrf"
                     /locus_tag="IV247_003014"
     rRNA            78..193
                     /gene="rrf"
                     /locus_tag="IV247_003014"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            207..279
                     /locus_tag="IV247_003015"
     tRNA            207..279
                     /locus_tag="IV247_003015"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:240..242,aa:Val,seq:tac)
     gene            283..355
                     /locus_tag="IV247_003016"
     tRNA            283..355
                     /locus_tag="IV247_003016"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:316..318,aa:Lys,seq:ttt)
     gene            363..444
                     /locus_tag="IV247_003017"
     tRNA            363..444
                     /locus_tag="IV247_003017"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:397..399,aa:Leu,seq:tag)
     gene            462..534
                     /locus_tag="IV247_003018"
     tRNA            462..534
                     /locus_tag="IV247_003018"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:495..497,aa:Thr,seq:tgt)
     gene            546..617
                     /locus_tag="IV247_003019"
     tRNA            546..617
                     /locus_tag="IV247_003019"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:578..580,aa:Gly,seq:gcc)
     gene            634..719
                     /locus_tag="IV247_003020"
     tRNA            634..719
                     /locus_tag="IV247_003020"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:668..670,aa:Leu,seq:taa)
BASE COUNT          175 a          168 c          199 g          181 t
ORIGIN      
        1 actcggaaaa aaacttatgt gacttcaaat ccagttttga gtgaacaaaa tttactcaat
       61 aaaagataac accacagtgt ggtggcgata gcgagaagga tacacctgta accatgccga
      121 acacagaagt taagcttctt agcgccgatt gtagtgaggg gttgcccctt gtgagagtag
      181 gacgtcgcca cgcttaagtt atccatggag gtttagctca gctgggagag catctgcctt
      241 acaagcagag ggtcagcggt tcgatcccgt taacctccat atgagccgtt agctcagttg
      301 gtagagcatc tgacttttaa tcagagggtc acaggttcga gccctgtacg gctcatattt
      361 ttgcgggtgt ggcggaattg gcagacgcac tagatttagg atctagcgcc ttacggcgtg
      421 ggggttcaag tcccttcacc cgcataattt aagttttaca tgccggctta gctcagttgg
      481 tagagcatct gatttgtaat cagagggtcg agggttcaag tcctttagcc ggcatcttta
      541 aacacgcgga aatagctcag tggtagagca ccaccttgcc aaggtggggg tcgcgggttc
      601 gaaccccgtt ttccgctttg ccaaagcaat cgcgccgggg tggcggaact ggcagacgca
      661 caggacttaa aatcctgcgg tgagtgatca ccgtaccggt tcgattccgg tcctcggcat
      721 ttc
//
LOCUS       DACDIG010000237          692 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.245, whole genome shotgun sequence.
ACCESSION   DACDIG010000237 DACDIG010000000
VERSION     DACDIG010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.245"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            134..346
                     /locus_tag="IV247_003021"
     CDS             134..346
                     /locus_tag="IV247_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778994.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            508..>692
                     /locus_tag="IV247_003022"
     CDS             508..>692
                     /locus_tag="IV247_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778995.1"
                     /translation="MNKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVL"
BASE COUNT          247 a          103 c          120 g          222 t
ORIGIN      
        1 taaaaacact atatattccg aaaagtgagt atctccattc ataaaataat caacatattt
       61 aaaaaatcta attagctttt ttagtcaatc taaagttctt tattctatga tataatgtta
      121 ctgtagaaag tttgtggcgg tttggcaaat ccaattgaag gagcgtggtg cctatgggct
      181 tattagctta tcggacgtac tctgtagaaa ggaaatgcct gatttgttct cattggatca
      241 aatcattgct attatcggag cagcagcagt agttattact gccgtagcga aagctagcga
      301 ttcgatttta gcttggttaa agttcaaaaa agaacaaaaa aaatagagcc gtctcaactt
      361 ttggcgaggt agacgagctc taaagcttta tttacatgcc atccgctttg aacggactac
      421 actgggcatg tgacagcatg ccctttcatt tatattatat ctcattcttt tatataatga
      481 aagtaaataa atcatggagg ataaaatatg aataaattta atagattaac attaataaat
      541 cagtttgaaa ttttaaaagc tttaaatcct aacgaagaaa aatactatgc tgaaaaaata
      601 gaaatactaa cagaaggtta cgaatatcac tatagtgaaa tatttgaaaa tatttctgat
      661 ccattgccag aagaagattc gcggtttgtt ct
//
LOCUS       DACDIG010000238          679 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.246, whole genome shotgun sequence.
ACCESSION   DACDIG010000238 DACDIG010000000
VERSION     DACDIG010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.246"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..>679)
                     /locus_tag="IV247_003023"
     CDS             complement(<1..>679)
                     /locus_tag="IV247_003023"
                     /inference="COORDINATES: protein motif:HMM:NF024832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP8778996.1"
                     /translation="IISLGSLVGNFIFFFSLKKYLDFDKIVGLDLSNLRPFINSIVIL
                     LVPQVAIQLYQYIIKFSIENSTTTTELSYFDQSQKIARIIIQLVSSISIVLMPVIANL
                     DKNKSRNALNKIIQLSVNYSLSIAVIFTILLVIISPTFVPIFFGPEFKEMIPHMIISS
                     FIIIPICVGGVFSNQLILGMGLYKYYATPYLIGGSIGILICLIYKDKMSGLIGCIVLV
                     IVESLVCF"
BASE COUNT          276 a          100 c           87 g          216 t
ORIGIN      
        1 aaagcaaaca agtgattcta ctataactag tactatacat ccaattaatc cactcatttt
       61 gtctttataa attaagcaaa ttaaaattcc tatacttcca cctataagat atggtgtagc
      121 ataatattta tacaacccca tccctaaaat gagctggtta gaaaagactc cacctacaca
      181 aattggaatt ataataaaag atgagattat catatgaggt atcatttctt taaattcagg
      241 accaaaaaaa attggaacga aagtgggtga aattattaca agcaaaattg taaatatcac
      301 cgctatactt aatgaataat ttaccgacaa ctgaataatt ttattcagag catttctgga
      361 tttattttta tctagatttg caatgactgg cattaataca atactaattg aactaactaa
      421 ttgaatgatt attctagcaa ttttttgaga ttgatcaaaa taagataact cagtagtcgt
      481 agtagaattt tcaatactaa attttattat atactgatat agctgaatag ccacttgagg
      541 aacaagcaat attacaattg aattaataaa tggccttaag ttagataaat ctaaaccgac
      601 aatcttgtcg aaatcgagat atttttttaa ggagaagaaa aatataaaat taccaactaa
      661 cgatcccaaa gaaataata
//
LOCUS       DACDIG010000239          662 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.247, whole genome shotgun sequence.
ACCESSION   DACDIG010000239 DACDIG010000000
VERSION     DACDIG010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.247"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            7..624
                     /locus_tag="IV247_003024"
     CDS             7..624
                     /locus_tag="IV247_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8778997.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
BASE COUNT          242 a           86 c          123 g          211 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 ca
//
LOCUS       DACDIG010000240          656 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.248, whole genome shotgun sequence.
ACCESSION   DACDIG010000240 DACDIG010000000
VERSION     DACDIG010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.248"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(138..491)
                     /gene="tnpB"
                     /locus_tag="IV247_003025"
     CDS             complement(138..491)
                     /gene="tnpB"
                     /locus_tag="IV247_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAP8778998.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          198 a          143 c          111 g          204 t
ORIGIN      
        1 ttgaagcaac tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa
       61 ctgattttgt tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt
      121 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
      181 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      241 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      301 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      361 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      421 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      481 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      541 gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc
      601 cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaa
//
LOCUS       DACDIG010000241          645 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.249, whole genome shotgun sequence.
ACCESSION   DACDIG010000241 DACDIG010000000
VERSION     DACDIG010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.249"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>645
                     /locus_tag="IV247_003026"
     CDS             <1..>645
                     /locus_tag="IV247_003026"
                     /inference="COORDINATES: protein motif:HMM:NF012745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8778999.1"
                     /translation="PKVSIIIPAYNAEYTISRALESALKQTYSNKEIIVIDDGSKDRT
                     LAICKEYQLNNHEIFIFSQKNSGVSVARNLGIRKADGEFIQFLDADDAMEPNMTEKLI
                     EVQRNNDSDYVICGLTIIKDNNIVRTPHLKERNINFINFEDYKYIYPLLAAPCNKLFK
                     KELIVLFEKNFSNGEDLLFNTEYLTKIKKISIIEDCLYKVYLDNENSLNKKMSIS"
BASE COUNT          263 a           76 c           98 g          208 t
ORIGIN      
        1 gccaaaagta tctataatta ttcctgcata taatgctgag tatactattt cgagagctct
       61 cgaaagtgca cttaaacaaa cttattctaa taaagaaata atagtcattg atgatgggtc
      121 taaagataga acattagcaa tctgtaaaga gtatcaatta aataatcatg aaatatttat
      181 attctcacaa aaaaattcag gtgtatcagt agctagaaat ttgggaataa gaaaagctga
      241 tggagaattt atacaatttt tagatgctga cgatgctatg gaacctaata tgactgagaa
      301 acttattgaa gttcagcgca ataatgattc tgactatgta atttgtgggt taactattat
      361 aaaagataat aatatagtga gaaccccaca tctaaaggaa agaaatatca attttattaa
      421 ttttgaagat tataaatata tctatccttt gcttgctgca ccatgcaata aactttttaa
      481 aaaggagctt attgtactat ttgaaaaaaa ttttagcaat ggagaagact tgttgttcaa
      541 tacggaatat ttaacaaaaa taaaaaaaat ttcaatcatt gaagactgtt tatataaggt
      601 atacttagat aatgaaaatt ctttaaacaa aaaaatgagt atatc
//
LOCUS       DACDIG010000242          631 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.251, whole genome shotgun sequence.
ACCESSION   DACDIG010000242 DACDIG010000000
VERSION     DACDIG010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.251"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>631
                     /locus_tag="IV247_003027"
     CDS             <1..>631
                     /locus_tag="IV247_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAP8779000.1"
                     /translation="YDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTL
                     PAYKRTNDTLETTIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAF
                     KSRALNDKYVAIFMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYV
                     WNELLQDINSRGVQEVLLFITDGLKGMKDTIHQIYPKAKYQHCCIHVSR"
BASE COUNT          224 a          117 c          110 g          180 t
ORIGIN      
        1 aatatgatcg cactggtttt aattcaggta attcgagaaa cggttcttac tttcgatcaa
       61 tcaaaaccga atatggtgaa ttaacattgg aaatacctag agatcgtaat ggtgagttta
      121 aacaacaaac tttaccagcc tacaaaagaa caaacgatac attggaaacc actattatcc
      181 atttattcga aaaaggtgtt acgatgtctg aaattgctga tttgatcgaa aaaatgtacg
      241 gtcatcacta tactccacaa accatgtcca acatgactaa agttctgact gaagaagtaa
      301 atgcctttaa atccagagcc ttaaatgata agtatgtcgc tatttttatg gacgctactt
      361 acattccact aaaacgtcaa accgtatcca aagaagcgat ttatattgcc attggtatac
      421 gagaagacgg cactaaagaa gtactgagtt atgcgattgc tccaactgaa tcaacatacg
      481 tttggaatga gctgctacag gatattaact ccagaggagt tcaagaagtc ttgcttttta
      541 ttacggacgg cttaaaaggc atgaaagata ctatccatca aatttatcct aaagcaaaat
      601 atcagcattg ttgtatccat gtatctcgta a
//
LOCUS       DACDIG010000243          605 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.252, whole genome shotgun sequence.
ACCESSION   DACDIG010000243 DACDIG010000000
VERSION     DACDIG010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.252"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>605
                     /locus_tag="IV247_003028"
     CDS             <1..>605
                     /locus_tag="IV247_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8779001.1"
                     /translation="ILLHETANETATGRNEAQYMKRSWYNAYTAYIVGDGGIVYQVGQ
                     PGYVQYGAGSYANANSPVQIELQHTHDKVTFEKNYKAYVELARDSANKYGIPLTLDTP
                     YNQPGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPV
                     IDPTRAGAANPTLSDGTNHSHIDQFGEIENANLHVAGWHIA"
BASE COUNT          206 a          124 c          128 g          147 t
ORIGIN      
        1 atcctattgc atgaaacagc aaacgaaact gcgacaggac gaaatgaagc gcagtacatg
       61 aaacgttctt ggtacaatgc ctatacagcg tatattgttg gcgatggtgg gatcgtttac
      121 caagttggac aacctggtta tgtacaatat ggcgctggtt catatgctaa tgcaaatagt
      181 cctgtgcaaa tcgaactgca acatacgcac gataaagtaa cgtttgaaaa gaactacaag
      241 gcatatgttg aattagcgag agattccgct aataaatacg gtattccact tacattagac
      301 acaccgtata atcaaccagg aatcaaatca catttatggg taactcaaaa catttggggc
      361 gatcacactg atccgtatgg atacttatca gaaatgggtg taagtaagga aaaactggct
      421 tacgatttag ctcatggatt tacagacgaa aacccaacga cttctgagaa caagcctgtc
      481 attgatccaa cccgagcagg tgcagcaaat ccaactttat cagatggaac gaaccattct
      541 cacattgatc agtttggaga aatcgaaaat gcgaacttac acgtcgctgg atggcacatc
      601 gctaa
//
LOCUS       DACDIG010000244          603 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.253, whole genome shotgun sequence.
ACCESSION   DACDIG010000244 DACDIG010000000
VERSION     DACDIG010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.253"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..214
                     /locus_tag="IV247_003029"
     CDS             <1..214
                     /locus_tag="IV247_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase APH(3')"
                     /protein_id="HAP8779002.1"
                     /translation="LEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPIETI
                     VYGIKNIKQEFIENGRKEIYKRTYKD"
     gene            complement(344..>603)
                     /locus_tag="IV247_003030"
     CDS             complement(344..>603)
                     /locus_tag="IV247_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8779003.1"
                     /translation="KYSKACASLDDGFEDAFQYTVQGNSHNRLKSTNLIERLNQEVRR
                     REKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          229 a           66 c          108 g          200 t
ORIGIN      
        1 acttgaagat agtgaagaag aaataggaac aaattttgga gaagatatat taagaatgta
       61 tggaaatata gatattgaga aagcaaaaga atatcaagat atagttgaag aatattatcc
      121 tattgaaact attgtttatg gaattaaaaa tattaaacag gaatttatcg aaaatggtag
      181 aaaagaaatt tataaaagga cttataaaga ttgattatat aatatatgaa aagctattat
      241 aaaagacatt agtattaaat agtttaaaaa aatgaaaaat aataaaggaa gtgagtcaat
      301 tccagactcc tgtgtaaaat gctatacaat gtttttacca tttctactta tcaaaattga
      361 tgtattttct tgaagaataa atccattcat catgtaggtc cataagaacg gctccaatta
      421 agcgattggc tgatgtttga ttggggaaga tgcgaataat cttttctctt ctgcgtactt
      481 cttgattcag tcgttcaatt agattggtac tctttagtcg attgtgggaa tttccttgta
      541 cggtatattg aaaggcgtct tcgaatccat catccaatga tgcgcaagct tttgaatatt
      601 ttg
//
LOCUS       DACDIG010000245          596 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.254, whole genome shotgun sequence.
ACCESSION   DACDIG010000245 DACDIG010000000
VERSION     DACDIG010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.254"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            61..>596
                     /locus_tag="IV247_003031"
     CDS             61..>596
                     /locus_tag="IV247_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP8779004.1"
                     /translation="MEIFGFYDTAFHINRALSLESIFSSPINFETFRSYGMQVNNFYP
                     WLTLYPLFLLIKFTNLAIGYNLFLYIVTLITLFICHYVMYEITKKHVTSSFFAIIYTT
                     SSFRSVEIFLRGAMGELLAMSILPLILLGFIKLYDSKKESWVMLAISMTLLIYTHVLS
                     VAMTMVMIIIALIIQFYR"
BASE COUNT          178 a           86 c           83 g          249 t
ORIGIN      
        1 aaaatattta tagtttatgc atttgttagt ttattatatt tatttccagc atttaaaaac
       61 atggaaatat ttggctttta tgatacggca tttcacatta atcgtgcgtt aagtttagaa
      121 tctattttta gctcccctat taattttgaa acgtttagat catatggcat gcaagtaaat
      181 aatttttacc catggttaac tctatatcct ttatttttat tgatcaagtt tactaattta
      241 gcaataggct ataacctttt tctttatatt gttacgctca ttactctatt tatatgtcat
      301 tatgtaatgt atgaaattac caaaaaacat gttactagct cctttttcgc tattatatat
      361 acaacatctt cttttaggag tgtagaaata tttcttcgag gggcaatggg ggaattactc
      421 gcaatgtcaa tacttcctct tattttactc gggtttataa agctttacga ctcaaaaaaa
      481 gaatcttggg ttatgttggc gattagcatg actttattaa tttacactca tgttctttct
      541 gtagctatga ctatggtaat gattataatt gctttaatta ttcaatttta tcggaa
//
LOCUS       DACDIG010000246          591 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.255, whole genome shotgun sequence.
ACCESSION   DACDIG010000246 DACDIG010000000
VERSION     DACDIG010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.255"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(316..579)
                     /locus_tag="IV247_003032"
     CDS             complement(316..579)
                     /locus_tag="IV247_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circular bacteriocin, circularin A/uberolysin
                     family"
                     /protein_id="HAP8779005.1"
                     /translation="MKKNLLLVLPILGFAGFFVGVPMLSANIGISSYAAKKVIDIINT
                     GSTVATIISIVAAVVGGGLITAGIVATAKSLIKKYGAKYAAAW"
BASE COUNT          224 a          103 c           78 g          186 t
ORIGIN      
        1 cttccttttt tgtatggatt ttttcattta cacaaaatat tttatactct cctgaatttt
       61 ttcatgttga aaacatcact aatcaaaggc aaattataat ttagaaatag aatgaaaaca
      121 ctaaatgaaa atagaacatt agaaaaaata gaatcaaaaa aacccaagac tagtgccgaa
      181 ataagggaaa caatcgaagc tacaaaaaat aatgatttcg ttgaacagga ccaatttatt
      241 tgtttccgat atttctcaaa aagaactttc tttgggaaag ttaaaggcga ctgtaaagtc
      301 gcctttagtc cttttttacc aagctgctgc atatttagct ccatattttt taattaatga
      361 tttggctgta gcaacaatac cagctgtaat taatccgcct ccaacaactg cagcaacaat
      421 agaaatgatt gtagcaaccg tgctaccagt attaattatg tcaataactt ttttagctgc
      481 ataagaagaa attccaatgt tagcacttag cattggtaca ccaacaaaga atcccgcaaa
      541 acctaaaatt ggtaaaacta ataataaatt ctttttcata ataattcctc t
//
LOCUS       DACDIG010000247          572 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.256, whole genome shotgun sequence.
ACCESSION   DACDIG010000247 DACDIG010000000
VERSION     DACDIG010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.256"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..441)
                     /locus_tag="IV247_003033"
     CDS             complement(<1..441)
                     /locus_tag="IV247_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP8779006.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSL"
BASE COUNT          195 a           96 c           84 g          197 t
ORIGIN      
        1 gagggaacag tctacaaaat ctgcgcttga taagacagag tgctcaaagg tactttcttt
       61 tatgtttgaa ccagataaat ttacatagtc cattttacaa ttctcgaata acgtgttccc
      121 aatataactt tccatcaatt tagtacctgt taatttacaa ttgacaaatt tcacgcgatg
      181 caacatgctt tttgtcaata tacagcctga aaaatcacag ttttcgaata tacagtcggt
      241 aatctccatt ctttgaaaat cattggaagc aaaaaatgca tacttgaaaa tagatttgta
      301 aataattgga tggcagatta tttggttttc gatgacctct ttttgtgaaa ggatattata
      361 cagttcaaaa tcttcgtttt catagatatg aaaaaaggat cctttattca agcttgcttc
      421 attaacttta ggaaaaatca tatttaatta cctccatttt cataaagtca tattaacaaa
      481 agctaaacca acttacatta gcagaaaatg agataggttt tcgccttttt cgataacgat
      541 agtgtcgcta tctcttgaaa cgttcttaga at
//
LOCUS       DACDIG010000248          536 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.258, whole genome shotgun sequence.
ACCESSION   DACDIG010000248 DACDIG010000000
VERSION     DACDIG010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.258"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            51..>536
                     /locus_tag="IV247_003034"
     CDS             51..>536
                     /locus_tag="IV247_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HAP8779007.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YL"
BASE COUNT          194 a           84 c          108 g          150 t
ORIGIN      
        1 tactatcaaa taaggtagaa taatagaaaa agatagcagg aggaatgacg atgaatcatt
       61 ttaaaggaaa gcaatttcag caggatgtga ttattgtagc cgtgggctac tatcttcgtt
      121 ataaccttag ctatcgtgaa gttcaagaaa tcttatatga tcgtggcatt aacgtttctc
      181 atacgacgat ttatcgttgg gtgcaagaat atggcaaact actctatcaa atttggaaaa
      241 agaaaaataa aaaatccttt tattcatgga aaatggatga aacgtacatc aaaattaaag
      301 gaaaatggca ttatttgtat cgagccatcg atgcagatgg tttaaccttg gatatttggt
      361 tacgtaaaaa acgggacaca caagcagcct atgcttttct taagcggtta gtgaagcagt
      421 ttgatgaacc gaaggttgta gtcacagata aagccccctc tattacaagt gcctttaaga
      481 aactaaaaga atacggcttt tatcaaggga cagaacatcg taccattaaa tacctg
//
LOCUS       DACDIG010000249          529 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.259, whole genome shotgun sequence.
ACCESSION   DACDIG010000249 DACDIG010000000
VERSION     DACDIG010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.259"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>529
                     /locus_tag="IV247_003035"
     CDS             <1..>529
                     /locus_tag="IV247_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8779008.1"
                     /translation="RPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPISCSARTIYRM
                     FKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEFGHLEGDTIV
                     GLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFKSITFDCGKE
                     FSNWKQISNVNDIAI"
BASE COUNT          175 a           91 c          107 g          156 t
ORIGIN      
        1 gacgtccgct tgttttaccc gaggaacaat cagaatatat tcaaagaaag gttgttcaag
       61 gatggacacc cgatgtgatt gttggtcgtg cagcgtttcc tattagttgt tctgctcgta
      121 ccatttatcg tatgttcaaa aaggggctat ttgattcttc tgacttaccg atgaaaggca
      181 agcgtaaacc gaatggacat caagaaagac gtggaaaaca aactttccgc cgctctattc
      241 atgaacgtga aaaggattat agccaattct caaatgagtt tggtcacctt gaaggtgaca
      301 ctatcgtagg tctgaaacat aaaagtgctg taattacctt agttgaacga ttatcaaaag
      361 ttatcatcac attgaaaccg tgtggtagac aagcgattga tattgaaaaa aaattaaatc
      421 aatggtttga atctgtaccg aaaaacctat tcaaatccat cacttttgat tgtggaaagg
      481 aattttcaaa ttggaaacag atcagtaatg tcaatgatat tgccattta
//
LOCUS       DACDIG010000250          524 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.260, whole genome shotgun sequence.
ACCESSION   DACDIG010000250 DACDIG010000000
VERSION     DACDIG010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.260"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>524
                     /locus_tag="IV247_003036"
     CDS             <1..>524
                     /locus_tag="IV247_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8779009.1"
                     /translation="EINLLNEYYDEIIEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTH
                     NKKISYQYHVGQYLPDWHPWEDYRNFFVSNKKTNGCREIFAIELPWIIDTFGKVKSFT
                     VMTQKLSNLDIDYSDSFTLIIEHETGIVGTLSVDVVSRVAKRNLTIIGEKILIEWEGN
                     PDSLFSWNEETSSM"
BASE COUNT          196 a           61 c           91 g          176 t
ORIGIN      
        1 ggaaatcaat ttattaaatg aatattatga tgaaataatc gaagtagcta aaaagaaaga
       61 tagattactt tatctatctt ctacattttt gaagagaaaa gaaattcaat atattaagtc
      121 taaagtgacg cataataaaa aaatttctta tcaataccat gtaggacagt atttacccga
      181 ctggcatcct tgggaagatt atcgcaattt ttttgtttca aacaaaaaaa ctaatgggtg
      241 tagagaaata ttcgctattg aattaccttg gattatagat acatttggga aagttaaatc
      301 gtttactgta atgacccaga aattatccaa tcttgatatt gattattcgg acagtttcac
      361 tcttattatt gagcatgaaa ctggtatagt tggaacttta agtgtagatg ttgtttcaag
      421 agtagctaaa agaaatttaa ctataattgg tgaaaagatt ttaatagaat gggaaggtaa
      481 cccggattct ctattttcat ggaatgaaga aacttctagt atga
//
LOCUS       DACDIG010000251          520 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.261, whole genome shotgun sequence.
ACCESSION   DACDIG010000251 DACDIG010000000
VERSION     DACDIG010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.261"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(56..298)
                     /locus_tag="IV247_003037"
     CDS             complement(56..298)
                     /locus_tag="IV247_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779010.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            419..491
                     /locus_tag="IV247_003038"
     tRNA            419..491
                     /locus_tag="IV247_003038"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:452..454,aa:Thr,seq:cgt)
BASE COUNT          161 a          102 c           84 g          173 t
ORIGIN      
        1 acagctttcg tttgcatata aaaaaaccat cccaaaaatg cagagatggt caaattcaaa
       61 tcttttttag ttctttgtag ttaacgatta ctacaccctt actttgcgtc atcaagttcg
      121 cattttctgc agtttcatca atctcaacaa cagcggaatt tttcaattgc tttgaaatcg
      181 ttccagtttg cgacttgccg tgaattgtga acattacgtg agtattgatc ttgaattttt
      241 caccaatttc cgatggtacg atagttgtat caaattttcc aaaagttgcc attttcataa
      301 aacctgcctt tcacatcaat tataccacgt tttatgaaaa atatcatgaa attaaactaa
      361 ttttccatcg gactctttac aatcagccaa atagagtata taatttcttt tggataaggc
      421 cagcttagct cagttggtag agcaacgcac tcgtaacgcg taggtcgtag gttcgatccc
      481 tatagctggc attaaaagag aagacagata tatcaaggat
//
LOCUS       DACDIG010000252          514 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.262, whole genome shotgun sequence.
ACCESSION   DACDIG010000252 DACDIG010000000
VERSION     DACDIG010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.262"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..132)
                     /locus_tag="IV247_003039"
     CDS             complement(<1..132)
                     /locus_tag="IV247_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP8779011.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          186 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaa
//
LOCUS       DACDIG010000253          512 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.263, whole genome shotgun sequence.
ACCESSION   DACDIG010000253 DACDIG010000000
VERSION     DACDIG010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.263"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
BASE COUNT          174 a           98 c           59 g          181 t
ORIGIN      
        1 aacacagtta tttttaaaaa taagaatgct aaatcactct acaataacgg cgaaagcaca
       61 aaaaataaaa aaattattac gttattaaaa aaacagatga aagatcaggt tttattagat
      121 ctcattcttt catcacttga agggatttaa tgaactactt tttcctcttt gtcgttttta
      181 tatattcgta ttccttcaaa cagtaggata gcctctttca atgcttgata tttgaactgt
      241 ccgttactta aaaatttaga tgtgccgccc tcttcctaga acatattctt ttttactttt
      301 tcattaattg acagaccaat cgaacgaaac tgtaaatttc gataggataa tgattggtca
      361 aactcattca ttttcaagac atctcgattc atattcaaga taggaatctt tttatcaaca
      421 tcactttatt cgtattttcc tctaccataa aagcttcacg ctccacaaaa ttgttttata
      481 cactactatt cagcccattc aacgaccaat ga
//
LOCUS       DACDIG010000254          487 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.264, whole genome shotgun sequence.
ACCESSION   DACDIG010000254 DACDIG010000000
VERSION     DACDIG010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.264"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..413)
                     /locus_tag="IV247_003040"
     CDS             complement(<1..413)
                     /locus_tag="IV247_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779012.1"
                     /translation="MGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEINRKIYWVS
                     IPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFSLTTPGN
                     ALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPF"
BASE COUNT          184 a           78 c           62 g          163 t
ORIGIN      
        1 aaaaacggct ttccaaatga tgaaatacct aaatctaact tattaagaaa gttgaaattt
       61 ttatattcag gaaaccattt attaatttca tgtgcagcac gtaatgcatt ccctggagtt
      121 gtcaaagaga aaattaattc tgctaagctt attattgaaa atactgataa cttataatta
      181 tatcgtccat tgacaatcaa atataaacta actatacttg ttagaacaaa aaaacaggcg
      241 ttgacttgtt cttgattagt taaaaatatc aataaaggaa tacttaccca atatattttt
      301 ctatttattt cttctcctct taagagttga aaaaacggat aaaaagttag taaactaaat
      361 gctactggcc actgatagtt caaggtggtt gctctccaac cggtttcccc cattactgtc
      421 aatggtatta aacagtatat agagcaaacc aataataaat ttttaacatt tattttctca
      481 aaaacat
//
LOCUS       DACDIG010000255          482 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.265, whole genome shotgun sequence.
ACCESSION   DACDIG010000255 DACDIG010000000
VERSION     DACDIG010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.265"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            7..>482
                     /locus_tag="IV247_003041"
     CDS             7..>482
                     /locus_tag="IV247_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP8779013.1"
                     /translation="MESYQFQVFKLVPSLERRGNRMKITEKNVNPKFETTESNLKKFD
                     LTVTLSNQLVDSIVEMDAMPQKIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSS
                     SSKHSRLKQVIEKLHQQSVIKIQRTKPNGKTEYKVISPLEITEFNDFSDAVSIKFS"
BASE COUNT          182 a           82 c           70 g          148 t
ORIGIN      
        1 tattcaatgg aatcgtacca gttccaagtt tttaagctcg taccaagctt agaaagaaga
       61 ggcaatcgta tgaaaataac agaaaagaat gtaaacccga aatttgaaac taccgaaagc
      121 aatctaaaaa agtttgattt aactgttact ttatcgaatc aacttgtaga tagcattgtt
      181 gaaatggatg ctatgccaca aaaaatttat ctttacgcta taggacatct tgaccctaaa
      241 aatccgcctt ttaataatac cgtcaaagta tctaaaaagg ctttatttgc gttgatgtct
      301 tctagttcat cttcttcaaa acattcaaga ttaaaacaag ttattgaaaa attgcatcag
      361 caatctgtca ttaaaattca aagaactaaa cctaacggta agactgaata caaagtcatc
      421 tcaccgctag aaattactga attcaatgat ttttctgatg cagtatctat taaatttagt
      481 ag
//
LOCUS       DACDIG010000256          473 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.266, whole genome shotgun sequence.
ACCESSION   DACDIG010000256 DACDIG010000000
VERSION     DACDIG010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.266"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(114..413)
                     /locus_tag="IV247_003042"
     CDS             complement(114..413)
                     /locus_tag="IV247_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP8779014.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          144 a           94 c           57 g          178 t
ORIGIN      
        1 atctgtttct ttatttagaa taaaaaaata cagactctgt tcaaataaat gaacctagcc
       61 tgcattatga gattgactat actttttaat tcattgagat gaataactcg tttttataaa
      121 tgcccaaaag taatcggtgc cgaagccatt cctcgaccta attctttttc aaattttcct
      181 attgttgata tctccatata aaacggtgat actggtttag acaattctcc cctgattgct
      241 aacggacgta aactacgttc caaattattt atgactttag gataatactg acctttttct
      301 aagcctgtct ttgcgtctat caaaagtata cgttcctttt ctttgatctc tggatttaaa
      361 acgagatcat ataattttag taccatgttt tctatttctt cacttttaga catcacgatt
      421 tcctcccaaa aatatatttc ttcccactac attctataaa caaagtatac gct
//
LOCUS       DACDIG010000257          469 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.267, whole genome shotgun sequence.
ACCESSION   DACDIG010000257 DACDIG010000000
VERSION     DACDIG010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.267"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..445)
                     /locus_tag="IV247_003043"
     CDS             complement(<1..445)
                     /locus_tag="IV247_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP8779015.1"
                     /translation="MNLVPLKNFNAKQMDLFFALCARMKDRGLKKVRFSFEELKELSD
                     YKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEINKTKKFVEVSVNPD
                     LEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYYVV"
BASE COUNT          170 a           86 c           65 g          148 t
ORIGIN      
        1 gaactacata ataaccagta gaacggtact gcataagcag tcggaataga gtttttgcat
       61 acgtagaacg caacgacgtg aaagcagata attcaaatct actaaattca gctgttaaac
      121 cattaataat atattctagg tccggattaa cactgacttc aacaaacttt tttgttttat
      181 tgatttcaaa accagtaaac aaaacaaagt agcgaataga atcctcatct tcagtacggt
      241 aagtaagatt aagcatatct ttatagagct tttctaaatc accgacaaag gcttttgtag
      301 cagtcatctt ataatcactt aactctttta attcctcgaa agaaaatcta acttttttca
      361 aaccacgatc tttcatacga gcgcataatg caaaaaataa atccatctgc ttagcattga
      421 aatttttcag cggtactaaa ttcaattcat tttgatattt gactgttaa
//
LOCUS       DACDIG010000258          463 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.268, whole genome shotgun sequence.
ACCESSION   DACDIG010000258 DACDIG010000000
VERSION     DACDIG010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.268"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
BASE COUNT          146 a           90 c           58 g          169 t
ORIGIN      
        1 aattcgcttg tggcgaaacc cgacctggag tttgtctctg tattttgaac cctaaaaatt
       61 aatcaaacag actgatattc tcataatcag cgcgaaataa gccattaagt acagttttcc
      121 tacctatttt tgataacccg ctggttagtt aatattcctt ttccgagaga gataaatgat
      181 tggttaaatc tatatacatc tcaaccttta aaaatgtttt cgaaatactt catcagtaca
      241 tgtcatacaa attaccagtg cattctcaaa ttctagttct ttaattgatt tcagttgagg
      301 agcagttact tttggcggaa atttccattt ttgtccaaac ctcttccccc gatgaaattt
      361 ctttgttgca attattgcaa tgaaaaattt ttgtttcaaa aatatacata attacttcct
      421 ccaataaaaa ctatctacct ctcaaattat ttttctctaa taa
//
LOCUS       DACDIG010000259          463 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.269, whole genome shotgun sequence.
ACCESSION   DACDIG010000259 DACDIG010000000
VERSION     DACDIG010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.269"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..364
                     /locus_tag="IV247_003044"
     CDS             <1..364
                     /locus_tag="IV247_003044"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779016.1"
                     /translation="YITYSVDNTVRCYEIKVTLSDLKSKAKQTFIGDYNYLVVTSDLW
                     EKMLQDDTLMRKYYKQGVYVFNEKYPSMISVKKARKQKVSLGTRSTVLESMVRSMNRE
                     VKKLYKVKPFWEIVEIPE"
BASE COUNT          181 a           55 c           95 g          132 t
ORIGIN      
        1 ttatataact tattcagtag ataacacagt tcgatgttat gagatcaaag ttactttatc
       61 cgatttgaaa agcaaggcga aacagacatt tatcggcgat tataactatt tagtcgttac
      121 tagtgatttg tgggagaaaa tgctacaaga cgacacactc atgcgtaaat attataaaca
      181 aggcgtatac gtgtttaatg aaaaatatcc tagcatgata agtgttaaga aagcaaggaa
      241 acaaaaagtt tcgctaggta caagatcaac tgtattagaa agcatggtta gatcgatgaa
      301 tcgtgaagtt aagaaattat ataaagtaaa accattttgg gagatagtag agataccgga
      361 atgatgagta aggaatatta tatctattca tatatagtat taactgatta tgaggaggat
      421 acagcaaatg aaaaattatg atccgaatat gcgatggggt aca
//
LOCUS       DACDIG010000260          462 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.270, whole genome shotgun sequence.
ACCESSION   DACDIG010000260 DACDIG010000000
VERSION     DACDIG010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.270"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..430)
                     /locus_tag="IV247_003045"
     CDS             complement(<1..430)
                     /locus_tag="IV247_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8779017.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          132 a           92 c           59 g          179 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tc
//
LOCUS       DACDIG010000261          455 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.271, whole genome shotgun sequence.
ACCESSION   DACDIG010000261 DACDIG010000000
VERSION     DACDIG010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.271"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            65..406
                     /locus_tag="IV247_003046"
     CDS             65..406
                     /locus_tag="IV247_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779018.1"
                     /translation="MEKELSTLDQYLTDPSWGKSNIKETNNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWDHVYLIHLSRKSKKPEYIYVIEDGLIDALEEYDRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
BASE COUNT          171 a           67 c           96 g          121 t
ORIGIN      
        1 aaaagtgact ccgccggcaa gcaaagagtc acaaagaaaa cacatcataa ggagatttta
       61 gcatatggaa aaagaacttt ccactctaga tcaatatttg actgatccta gttggggcaa
      121 atcgaatatc aaggaaacaa ataatcgaaa aatcagacga aatcttttga cggatgaaga
      181 actagcatgt gatcaagacg atttgggaaa ttttgtgagt atttgggatc atgtctatct
      241 tatccattta tcgaggaagt ccaaaaaacc tgaatatatc tatgtcatcg aagatggctt
      301 gattgatgcg ctagaagagt atgacagaga taacttgatt gatatctctt attacggatc
      361 aggtaagaaa tacattgctg aaatggaggc agaatttgat gagtgaaaac aaagggacaa
      421 cgacttttga aaaacttttt agtcgcaagt taaat
//
LOCUS       DACDIG010000262          452 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.272, whole genome shotgun sequence.
ACCESSION   DACDIG010000262 DACDIG010000000
VERSION     DACDIG010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.272"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>452
                     /locus_tag="IV247_003047"
     CDS             <1..>452
                     /locus_tag="IV247_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779019.1"
                     /translation="PEDKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDK
                     SDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVV
                     VTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEGEKVEVGGTVDLTDNVT"
BASE COUNT          187 a           66 c          104 g           95 t
ORIGIN      
        1 ccagaagata aacttccagt gttgaaagca aaagatggtt atacagatgc gaaatggcca
       61 gaggaagcaa ctcaaccaat tacagcagat gatacagaat ttgtatcaag tgcaacaaaa
      121 ctggatgata aatctgacgc tgacaaatat aatcctgaag gtcaaaaagt gactacagaa
      181 ttgaataagg aaccagacgc atttgaggga attaagaaca aagaagattt accaaaagat
      241 actaagtata cttggaaaga aaaagtagat gttagcgcag ctggaaataa aaaagggact
      301 gttgtagtga catatccaga tggatcatct gatgaagttg aagtagatgt tacagtgaca
      361 gacaatcgtt ctgacgccga taaatatgag ccaacagtag aaggcgaaaa agtagaagtt
      421 ggaggcacag tagatctaac agataatgta ac
//
LOCUS       DACDIG010000263          451 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.273, whole genome shotgun sequence.
ACCESSION   DACDIG010000263 DACDIG010000000
VERSION     DACDIG010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.273"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(36..272)
                     /locus_tag="IV247_003048"
                     /pseudo
     CDS             complement(36..272)
                     /locus_tag="IV247_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          148 a           75 c           77 g          151 t
ORIGIN      
        1 atagaaggac tgaccttctg tctcagattt ttttgttatt tcacttgaat caattgattg
       61 atcaaaaaaa tcctgatttt catgttctca aatgatttaa atccataaga taatcgtttc
      121 agtgtcttga tatggggaat cttggcctct attttgccat tagaataagg gtaaatcata
      181 gcattgtaaa taccttcttc atacgttaaa aggttttgaa gtttcgtgcg aagttgtgca
      241 tcaagtgttt caggaagatt ttttaataac gccgtttgta taattaagcg agacaaataa
      301 aaaagccatt tatgttacac tccaattgta atacccgcta aagcatacaa ggagagtgaa
      361 cataaatgac ttatacccat cttacaccaa acgagcttgt aatgatagaa gcatattttc
      421 atcaagaaac tccggttgct atcgttgcga a
//
LOCUS       DACDIG010000264          426 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.275, whole genome shotgun sequence.
ACCESSION   DACDIG010000264 DACDIG010000000
VERSION     DACDIG010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.275"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>426
                     /locus_tag="IV247_003049"
     rRNA            <1..>426
                     /locus_tag="IV247_003049"
                     /product="16S ribosomal RNA"
BASE COUNT          111 a           96 c          133 g           86 t
ORIGIN      
        1 gaaagccccc ggctcaaccg gggagggtca ttggaaactg ggagacttga gtgcagaaga
       61 ggagagtgga attccatgtg tagcggtgaa atgcgtagat atatggagga acaccagtgg
      121 cgaaggcggc tctctggtct gtaactgacg ctgaggctcg aaagcgtggg gagcaaacag
      181 gattagatac cctggtagtc cacgccgtaa acgatgagtg ctaagtgttg gagggtttcc
      241 gcccttcagt gctgcagcta acgcattaag cactccgcct ggggagtacg accgcaaggt
      301 tgaaactcaa aggaattgac gggggcccgc acaagcggtg gagcatgtgg tttaattcga
      361 agcaacgcga agaaccttac caggtcttga catcctttga ccactctaga gatagagctt
      421 cccctt
//
LOCUS       DACDIG010000265          408 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.276, whole genome shotgun sequence.
ACCESSION   DACDIG010000265 DACDIG010000000
VERSION     DACDIG010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.276"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..390
                     /locus_tag="IV247_003050"
     CDS             <1..390
                     /locus_tag="IV247_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8779020.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          158 a           73 c           64 g          113 t
ORIGIN      
        1 aaagaccgaa aagaaatctg tgatgacttt aaggctgttt atcaagctaa ctcaaaagaa
       61 gaagcgaata ccttcttatc cggcatgatt gagaaatgga agaaaaacta tcctaaagtg
      121 acgcagtcac tcatagaaaa ccaagactta ttaacttttt atgattttcc acctagcatt
      181 cgtagaacca tttactcaac caatctaatc gagtctttca ataagcaaat taaaagatac
      241 agccgtagaa aagagcagtt tcaaaatgaa gaatcactag aacgctttct agtcagcatt
      301 tttgatacat acaatcaaaa atttctaaac agaagccata aaggttttca acaggtaacc
      361 gatacattag tttcaatgtt tactgagtaa ctaattattt tgcaggag
//
LOCUS       DACDIG010000266          400 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.277, whole genome shotgun sequence.
ACCESSION   DACDIG010000266 DACDIG010000000
VERSION     DACDIG010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.277"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            126..400
                     /locus_tag="IV247_003051"
                     /pseudo
     CDS             126..400
                     /locus_tag="IV247_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT          155 a           49 c           70 g          126 t
ORIGIN      
        1 aaagaaggca ctctcttcgg gttttcggtc tgtactgaaa tcaaggtatt attgggaatc
       61 ccagcttaaa tcatagatac cgtaagggat tttattcttt atttaaaact ttgcaacaga
      121 accgtacaag atatattaaa ttgatttttg aatttttgaa aaaagaaaga aatgctaatg
      181 cagttatttt agaccctcat aaaaataatc caagagcaat aagggcatac caaaaatctg
      241 gttttagaat tattgaagat ttgccagaac atgaattaca cgagggcaaa aaagaagatt
      301 gttatttaat ggaatataga tatgatgata atgccacaaa tgttaaggca atgaaatatt
      361 taattgagca ttactttgat aatttcaaag tagatagtat
//
LOCUS       DACDIG010000267          387 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.278, whole genome shotgun sequence.
ACCESSION   DACDIG010000267 DACDIG010000000
VERSION     DACDIG010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.278"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..>387)
                     /locus_tag="IV247_003052"
     CDS             complement(<1..>387)
                     /locus_tag="IV247_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HAP8779021.1"
                     /translation="VYQKHDYPRINRNNTTAFKIFRAIINRCLRMIKLPPKIRHHKFD
                     LYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGMFAPDEKFFHTLFFNSSFKNKNV
                     IGGPDLPLELKNIEETTLQTSKLANI"
BASE COUNT          126 a           52 c           58 g          151 t
ORIGIN      
        1 tgaatatttg ctaatttcga agtttgtaaa gtagtttctt caatattttt caattcaagt
       61 ggtaaatctg gtcctccaat aacattttta tttttgaacg aggaattaaa aaaaagtgta
      121 tgaaaaaatt tttcatctgg ggcaaacata cctattttaa aattcaggta gtcttgttga
      181 ttttgttcat acatttgtat taattctgtt aggcattctt tcgacaaagc ccaccattga
      241 gaaccatgat acaaatcaaa tttatggtgt ctaatttttg ggggaagttt aatcatcctc
      301 agacaacgat taattatagc tctaaaaatt ttaaatgcag ttgtgttatt cctattaatt
      361 ctaggataat catgtttttg atataca
//
LOCUS       DACDIG010000268          384 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.denovo.279, whole genome shotgun sequence.
ACCESSION   DACDIG010000268 DACDIG010000000
VERSION     DACDIG010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.denovo.279"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>384
                     /locus_tag="IV247_003053"
     CDS             <1..>384
                     /locus_tag="IV247_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779022.1"
                     /translation="EKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMHEQREAL
                     RERIHYFDDIHTKIYEARTNHEDESKLGLAQQRILEWLNTNTDQEPLRVISNLFEFGT
                     KHWFNAVEKDADLQEAFDTLSKDSKS"
BASE COUNT          152 a           56 c           78 g           98 t
ORIGIN      
        1 gaaaagaaaa ttccagtgtt tttgtctgtc tcagaaatgt tatcacttca aatactgtta
       61 agaaaaaaga ttgaaacaat agacgaagaa gttagaaata aaagggctat gcacgaacaa
      121 cgtgaagcgc taagagaacg aatccactat tttgatgata tacacactaa aatctatgaa
      181 gcacgtacaa atcacgaaga cgaaagtaaa ttaggattag ctcaacaacg gatacttgaa
      241 tggcttaata cgaatactga tcaagaaccg ttaagagtga ttagtaatct ttttgaattt
      301 ggaacgaagc attggtttaa tgctgtagag aaggatgctg acttacaaga agcgtttgat
      361 acgctaagta aggatagcaa atcc
//
LOCUS       DACDIG010000269         5361 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.guided.114, whole genome shotgun sequence.
ACCESSION   DACDIG010000269 DACDIG010000000
VERSION     DACDIG010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.guided.114"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..765
                     /locus_tag="IV247_003054"
     CDS             <1..765
                     /locus_tag="IV247_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP8779023.1"
                     /translation="LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEA
                     MLKQAQDEPAIDEIYHFDFLEAKELGLKHNSSFYSKHMDFSSSEPKTDLFFGATNNGR
                     KERAEKYKQEFTKRGVTTNYFILPLRGNEQPGYLTYEEYLKKTGESRGILELLREGQQ
                     GVTLRTFESMYFQKKLVTDNQAIAHYQFYHSENIFLLQERDLDELPEFLHTPFQPIPS
                     ELLEFFDAENWAKRFFTTDSAIYEQYEYDLSSVKNK"
     gene            complement(908..1849)
                     /locus_tag="IV247_003055"
     CDS             complement(908..1849)
                     /locus_tag="IV247_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP8779024.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVVLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNKHVDLIVALTKANQLDDMFRSIQSIITENTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAEGM
                     VRTIVSEFASIAAKEGITLDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            1975..3096
                     /locus_tag="IV247_003056"
     CDS             1975..3096
                     /locus_tag="IV247_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP8779025.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTNWQGKNTAVIESAQVLGLDLLDKP
                     TDQTIRLASSYALGELIKDTLLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTRKLHLDRRNVLVSKVKVIIAADVTNPYTGQQGAAWQFGRQKGGTDTTLAFLD
                     KQAEKIAQQIKDVWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFELVSQITGLEKQI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDRELEGLFDFLPIV
                     LSIQLGPIDLEEAMEHRQTLEKTEILGQTLSRFFNILPK"
     gene            3354..4856
                     /gene="lsa"
                     /locus_tag="IV247_003057"
     CDS             3354..4856
                     /gene="lsa"
                     /locus_tag="IV247_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsa family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP8779026.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQAFVYFPQQTKDKERLTYYVLNEIMDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEIAKNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKKVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDALTMNSQVSHHKRLLSVENLQLGYENLLFEPVHF
                     TIEPHQRMAISGPNGAGKSSIIHYLLGTFNGKVVGEKSQPKHLTISYVRQNYEDNRGT
                     LAEFAKKNRVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYI
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLEKI"
     gene            4923..>5361
                     /locus_tag="IV247_003058"
     CDS             4923..>5361
                     /locus_tag="IV247_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP8779027.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQALAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVVDRLNLPMTADERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECMEDEENVYLLVCHNALI"
BASE COUNT         1751 a          964 c         1151 g         1495 t
ORIGIN      
        1 ttggcaaatt atcagctgat cgttttttta gattatggat ttgatatgga gatggctcga
       61 cgcgtgcgtc catttaccaa tgcaaaaatc gtgttatttt tttggaacca ctttaaagca
      121 gaacacgagg cgatgctgaa gcaggcacaa gatgaaccgg caatcgatga gatttatcat
      181 tttgattttt tagaagcgaa agaacttgga ctaaaacata atagttcctt ttattcgaaa
      241 catatggatt tttcttctag cgaaccaaag acggatctat tttttggtgc aacaaataac
      301 ggcagaaaag aacgggcaga aaaatataag caggaattta caaaaagagg tgttacgacc
      361 aattacttta ttcttccgct tcgaggaaat gaacaacctg gataccttac ttatgaagaa
      421 tatctgaaaa aaaccgggga atcgaggggg attctagaac tgttgcggga agggcagcaa
      481 ggagtgactt tgcgaacgtt tgaatctatg tattttcaaa agaaactagt gacagataat
      541 caagccatag ctcattatca gttttatcat tctgaaaata tttttttgct tcaagaacgt
      601 gatttagatg aattaccgga atttttgcat acgccatttc aaccaatccc atcagaactg
      661 ttggaatttt ttgatgcaga aaattgggca aaacgttttt tcactactga ctccgctatt
      721 tatgaacaat atgaatacga tctttcctct gttaaaaata aataaaagtg ccaaccaatc
      781 atagatgaaa ataaacaagc gcaattctta tggcaacatg agaattgtgt ttttcttttt
      841 gaactttcat gggggtaaaa aaatgagaac cagctgctga tccagctgat tctcatcaga
      901 gagcctttta ttctgcgcca aggattcctt cttttgcatg gactaattgg gtcaaaaagg
      961 cacaatatgg tgtagcaata tcatattttt gtccttttcg ccaaacggca ccattgatgt
     1021 agtcgatttc agtcaaacga tggtttttga tcaggtcttg gtacatagat gggtaatgca
     1081 acccgatgtt gtcaggatca taagtagaag caatatgttg gtacacttct tcttgatcta
     1141 atgtgattcc ttcttttgct gcgatagaag caaactcaga tacaatcgtt cgaaccatgc
     1201 cctctgccgc tttttgcgca cctaattcag caatattaca atccaatatc gtgcataatc
     1261 catttaatgt tccgtttaca caagcttttc gccaaatgga ataacgaaca ttatgactat
     1321 agattggatt caattttgct tcttgaaaga cttctacaac tttttttgta aatgcttcgc
     1381 cttctggttc caagttctct agttcgattt ctccatcacc taataatgtg acttttccgg
     1441 gaccagctag accagctgtc cacatcgtga tcccaaataa gatatttttc tttggtacat
     1501 atttttccag tacatcttca tgacctaatc cattcagtaa acacaaaaca taagtattct
     1561 cagtaataat ggattggatc gaacgaaaca tatcatctaa ttgattcgct tttgtcaagg
     1621 ctacgatcaa atccacgtgt ttgttgcttt cgatgatttc ttctggtgaa tagattggca
     1681 gttttgcaac tacttctttg ccattaaagt cagcaatcaa tccatttgta cgaatggctt
     1741 caatgtgagc aggccagcga tcgatcaaga cgacctcatt tccgctttga tgcaacatta
     1801 atccgattcg gctgcccatt gctccggctc cagctattgc gattttcatg ttttttctct
     1861 cctttattca atgagttccc ttttagtata tagaaaaaca gaagaaaata gcattgtttt
     1921 gtttgaaaaa agtgaaaaaa agtgtcttac ttaagaagaa agtgagggat atagatggat
     1981 attttggcgg caatcgattc atttaaagga agtgcttctt catttgaatt aaatcaggca
     2041 gttctaaatg gttgggaaga ccgtaaagga agaaaaatca atgtgccgat tgcagatggc
     2101 ggagaaaata ctgcagaagc aatctattac gcgctcgggg gaacctggcg tacgattgat
     2161 gggatggatc tcttatttcg tcagcgaaaa atcagttatt tattgacgaa ctggcaagga
     2221 aaaaataccg cagtcattga atctgcacaa gtattaggat tagatttact tgataaacca
     2281 acggatcaaa ccatccgact tgcttctagt tatgccttgg gagagcttat caaagataca
     2341 ctgcttcaag aagtggaaca aatcattgtc actttaggag gaagcggatg tagcgatggt
     2401 ggtctaggat tactccaatc tttgggtgct tctttagaag ggtacacaga aggaaatcct
     2461 ttgttatcta ctagaaaact tcatttggat cgaaggaacg ttcttgtttc taaagtaaaa
     2521 gtgatcatcg ctgcagacgt gaccaaccca tatacaggtc aacaaggagc tgcttggcaa
     2581 tttggaagac aaaaaggagg aacggatacg acgttagctt tcttggataa acaagctgaa
     2641 aagattgctc agcagataaa agatgtttgg gaggtggatt tagatgacat cccaggaagc
     2701 ggagcagcag gcggtattgg cggagccctt tttttgttag gagcgaagat ggtctctggt
     2761 tttgagttgg tcagccaaat cactggatta gaaaaacaga tcgaacaagc ggatgtcatc
     2821 attacaggag aagggcggat tgatcagcaa acaatccacg gaaaagttcc atatggcgta
     2881 gcagtgcttg caaaaaaata tcgaaaacca ataatcgcta tttgcggaag tagagaccga
     2941 gagctggaag gactctttga ttttctcccc attgtcttaa gcattcaatt aggaccaatc
     3001 gatttagagg aagcaatgga acacaggcaa acattagaaa aaacagaaat cttaggacag
     3061 acactcagca gatttttcaa cattctcccg aagtagttga caaaaacaat ttctttatgc
     3121 tatcgtaatt tcaaatatag gacaaagtga gggatccgtg cattgaaaaa ttaagtcgat
     3181 tgatgaaagc tgcattcaga ctgtaccttg tggagacaag ggagcagtgt gcagattttc
     3241 gggattggct tggttctttc gatgtatggg tctttttgtg tgccccaacc cttgcatcct
     3301 ctttagccag ttctgaaaat aggactggtc tttttttacg aggtgatgga ataatgtcta
     3361 aaatcgaaat aaaaaatctg acattcggct atgacagcca aggcacatta ttatttgaac
     3421 aagccaatct aaactttgac acacaatgga aactaggact tatcggacga aacggtcgag
     3481 gaaagacaac tttactgaat atcctgcaaa acaaactccc ttatcaaggg caagtgatcc
     3541 atcagcaagc gtttgtctat ttcccgcagc aaacaaaaga taaagagcgt ttgacctatt
     3601 atgtgttaaa tgagattatg gattttgaga tatgggaaat tgaaagagag ctccaactga
     3661 tgcaaacaga tcctgaaatc ttatggagag aattcagcac actatcagga ggagagaaga
     3721 caaaagtcct actggcactc ttatttgtgg atgacactca tttcccgtta atcgatgaac
     3781 caacgaatca tttggatatc tctggcagaa aacaagtagc ggcttacttg aaaaagaaaa
     3841 aacaaggatt catcgtggtg agtcatgatc ggggatttat cgatgaagta gtggaccatg
     3901 ttttagcaat cgaaaagagc caactggaac tttatcaagg gaatttctct atctatgaag
     3961 aacagaaaaa acttcgtgat gaatttgaaa tagctaaaaa tgaaaaattg aaaaaagaag
     4021 tcagcaggct aaagaaaaca gcagctgaaa aagcagaatg gtctcgttcc cgagaagggg
     4081 acaaaacaaa gaaaaaagtc ggattcatcg atacggaatc taggcgagtg aataaaggag
     4141 cagtaggcgc tgatgctgca cggacgatga aacgatctaa agcaatcgtg aatcggatgg
     4201 aaacccagat cagtgagaaa gaaaaattat taaaagatat cgagtatatc gatgctttga
     4261 cgatgaatag ccaagtgtct catcataaac ggctgttaag cgtagaaaat cttcaattag
     4321 ggtatgaaaa tctgttattc gagccagttc attttacgat cgaacctcat cagcggatgg
     4381 caatttcagg tcctaacggc gcgggaaagt catccattat ccattatctt ctggggacat
     4441 tcaacggcaa ggttgtagga gaaaaatccc agcccaaaca tttgaccatt agttacgtac
     4501 gccaaaatta tgaagacaat cgagggacat tggcggaatt cgcgaagaaa aaccgagtag
     4561 actatcaagc atttttgaat aatctccgaa agcttgggat ggaaagagat gtttttcata
     4621 ataaaatcga acagatgagt atgggacaac ggaaaaaagt ggaattggct aaatctttat
     4681 cccaaccagc tgagctctat atatgggatg aaccattgaa ttatttggat gtcttcaatc
     4741 aagaacaatt agaacaactg attttgaacg tgaaacctgc tatgttacta gtggaacatg
     4801 atcaaacctt tctagataaa gtatctactg agattatttc tcttgagaaa atctaaacct
     4861 tgtaatctca aagacaacaa ggtatggtta aggagaactt tgtcaacgaa ggagagaatc
     4921 acatgcttta tgtaacgaga cacggagaaa caacttggaa cgcacaagga ttagtttgcg
     4981 ggcgagcaga tgtgccgctg actgaaaaag gacagatgca ggcacaggca ctagcagaaa
     5041 aggtagtaga tttgcctgtt ccaatcacga aaatcatcca ttcccctctt caacgagcaa
     5101 gagatacagc acaagcagta gttgatcgtc tcaatttgcc gatgaccgct gatgagcgat
     5161 tggtagaaat ggattttggg gattatgacg gactgccaag caaagatgaa aactttcaaa
     5221 aagcgcgttt agcgtttgct gtacgtttcc caaatggaga gtcggtctta gatgtgtacg
     5281 cacggatcgt tcctttattg aaagaatgca tggaagacga agaaaatgtc tacttgttag
     5341 tctgtcataa tgccttgata a
//
LOCUS       DACDIG010000270         4104 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.guided.134, whole genome shotgun sequence.
ACCESSION   DACDIG010000270 DACDIG010000000
VERSION     DACDIG010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.guided.134"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            42..608
                     /locus_tag="IV247_003059"
     CDS             42..608
                     /locus_tag="IV247_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008790726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8779028.1"
                     /translation="MKQQELADKIGINMQSLSKIERGLNYPAYETLEKIMEVLDVTPN
                     ELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGHYDNFFDSEEEWLEYELEKLREYI
                     TDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDDLYSMDMFGESIEGHKYKTPYQVV
                     KMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            762..839
                     /locus_tag="IV247_003060"
                     /pseudo
     CDS             762..839
                     /locus_tag="IV247_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646598.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            1084..1200
                     /locus_tag="IV247_003061"
     CDS             1084..1200
                     /locus_tag="IV247_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAP8779029.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            1216..3135
                     /gene="tet(M)"
                     /locus_tag="IV247_003062"
     CDS             1216..3135
                     /gene="tet(M)"
                     /locus_tag="IV247_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAP8779030.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            3254..3421
                     /locus_tag="IV247_003063"
     CDS             3254..3421
                     /locus_tag="IV247_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8779031.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(3481..3834)
                     /locus_tag="IV247_003064"
     CDS             complement(3481..3834)
                     /locus_tag="IV247_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8779032.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
BASE COUNT         1413 a          624 c          820 g         1247 t
ORIGIN      
        1 atttaggaaa taatatacag accataagaa aatttagggg aatgaaacaa caggaacttg
       61 cggacaaaat cggtatcaat atgcagagcc tttccaagat tgaaagaggg ttgaactatc
      121 ctgcctatga aacgctggaa aagataatgg aagtgctgga cgttacgccc aatgaattgt
      181 tgtcgggaga atggaagtat gtcaatcaat ctgaaaagga agtctgtcag tttttaagag
      241 ccgaagaacg tctaaatgca gaactgaaac atggacacta tgataacttc tttgacagcg
      301 aggaagaatg gctggaatat gagttagaaa agttacggga atatattaca gactatatca
      361 acgggaaaag cattgaagcg tccgaccttt acccaatcaa agaatttatc cagcatttga
      421 agtttcaaaa attattagac cgctatgatg atttatacag tatggatatg tttggggaaa
      481 gcatagaggg acataagtat aagacacctt atcaagtcgt gaaaatgata aacccgaact
      541 caaaagaaga tatggaactg ttacgagaag tattaaggaa taacaatttt gatgatgagg
      601 acgagtaact tttttcctct tgcgagaaga tacagcaaac aaaaagcccg atcaagagtg
      661 caaagcacca ccaatggtgg caaagctctt gacagggctt tttcttttct gttgtgggca
      721 atcaagagga agaaagggat aaaatctgat ttgtggtaaa tcaggcaaag gcaacgcagg
      781 tatctcaatt tgatttggta cttgaaaaga acgggagtaa ttggaagatt atagaataac
      841 aaatattggt acattattac agctattttg taatcacgta ctctctttga taaaaaattg
      901 gagattcctt tacaaatatg ctcttacgtg ctattattta agtatctatt taaaaggagt
      961 taataaatat gcggcaaggt attcttaaat aaactgtcaa tttgatagtg ggaacaaata
     1021 attggatgtc cttttttagg agggcttagt tttttgtacc cagtttaaga atacctttat
     1081 catgtgattc taaagtatcc ggagaatatc tgtatgcttt gtatgcctat ggttatgcat
     1141 aaaaatccca gtgataaaag tatttatcac tgggattttt atgccctttt gggtttttga
     1201 atggaggaaa atcacatgaa aattattaat attggagttt tagctcatgt tgatgcagga
     1261 aaaactacct taacagaaag cttattatat aacagtggag cgattacaga attaggaagc
     1321 gtggacaaag gtacaacgag gacggataat acgcttttag aacgtcagag aggaattaca
     1381 attcagacag gaataacctc ttttcagtgg gaaaatacga aggtgaacat catagacacg
     1441 ccaggacata tggatttctt agcagaagta tatcgttcat tatcagtttt agatggggca
     1501 attctactga tttctgcaaa agatggcgta caagcacaaa ctcgtatatt atttcatgca
     1561 cttaggaaaa tggggattcc cacaatcttt tttatcaata agattgacca aaatggaatt
     1621 gatttatcaa cggtttatca ggatattaaa gagaaacttt ctgccgaaat tgtaatcaaa
     1681 cagaaggtag aactgtatcc taatatgtgt gtgacgaact ttaccgaatc tgaacaatgg
     1741 gatacggtaa tagagggaaa cgatgacctt ttagagaaat atatgtccgg taaatcatta
     1801 gaagcattgg aactcgaaca agaggaaagc ataagatttc agaattgttc tctgttccct
     1861 ctttatcatg gaagtgcaaa aagtaatata gggattgata accttataga agttattact
     1921 aataaatttt attcatcaac acatcgaggt ccgtctgaac tttgcggaaa tgttttcaaa
     1981 attgaatata caaaaaaaag acaacgtctt gcatatatac gcctttatag tggagtacta
     2041 catttacgag attcggttag agtatcagaa aaagaaaaaa taaaagttac agaaatgtat
     2101 acttcaataa atggtgaatt atgtaagatt gatagagctt attctggaga aattgttatt
     2161 ttgcaaaatg agtttttgaa gttaaatagt gttcttggag atacaaaact attgccacag
     2221 agaaaaaaga ttgaaaatcc gcaccctcta ctacaaacaa ctgttgaacc gagtaaacct
     2281 gaacagagag aaatgttgct tgatgccctt ttggaaatct cagatagtga tccgcttcta
     2341 cgatattacg tggattctac gacacatgaa attatacttt ctttcttagg gaaagtacaa
     2401 atggaagtga ttagtgcact gttgcaagaa aagtatcatg tggagataga actaaaagag
     2461 cctacagtca tttatatgga gagaccgtta aaaaatgcag aatataccat tcacatcgaa
     2521 gtgccgccaa atcctttctg ggcttccatt ggtttatctg tatcaccgct tccgttggga
     2581 agtggaatgc agtatgagag ctcggtttct cttggatact taaatcaatc gtttcaaaat
     2641 gcagttatgg aggggatacg ctatggctgt gaacaaggat tgtatggttg gaatgtgacg
     2701 gactgtaaaa tctgttttaa gtatggctta tactatagcc ctgttagtac cccagcagat
     2761 tttcggatgc ttgctcctat tgtattggaa caagtcttaa aaaaagctgg aacagaattg
     2821 ttagagccat atcttagttt taaaatttat gcgccacagg aatatctttc acgagcatac
     2881 aacgatgctc ctaaatattg tgcgaacatc gtagacactc aattgaaaaa taatgaggtc
     2941 attcttagtg gagaaatccc tgctcggtgt attcaagaat atcgtagtga tttaactttc
     3001 tttacaaatg gacgtagtgt ttgtttaaca gagttaaaag ggtaccatgt tactaccggt
     3061 gaacctgttt gccagccccg tcgtccaaat agtcggatag ataaagtacg atatatgttc
     3121 aataaaataa cttagtgtat tttatgttgt tatataaata tggtttcttg ttaaataaga
     3181 tgaaatattt tttaataaag atttgaatta aagtgtaaag gaggagatag ttattataaa
     3241 ctacaagtgg atattgtgtc ctgtatgtgg aaataaaaca cgattaaaga taagggaaga
     3301 tactgaatta aaaaaattcc ccctctattg tccgaaatgc agacaagaaa atttaattga
     3361 aataaagcag ttcaaagtaa ctgtgattac agagccagac gcaaagacgc agagccgata
     3421 aaatgagatt aatacaatct cattttatcg gctctttccg ttatgtatgg attcttttaa
     3481 ttagtcttcg atgtttcttg cttcgttgat accgctggct aaagattcca ttaaggatag
     3541 ttctttgtct gtaaagctat ccatgtattt ctctatctgt aatcgtcggg tgctttttac
     3601 caagttatta gcaggtaaga aaaattcatc aacggaaaca tgaagtaacg atacaaggtc
     3661 ataaagaact tgtatgctgg ggtgttgccc tttattttca atattagtta agtaccgtgg
     3721 gtcaatttca atcaatgctc ccacttgttc acgagttaaa cctcgtttca atcgagcttc
     3781 tttaatggct aaaccaaagg ctctaaaatc atatttatct tcttttttac gcatagtaga
     3841 ccacctctat acattttatt gttcctactg aattaaaaac aggtatagaa aaacgtgtta
     3901 tatggtttat aggtttatat ttaataaaaa gcactactaa acgccaataa aaaaaaccgt
     3961 tatatggtag tgctatttac gctgttaaaa tattgtatat tacttccaaa tggcggtttg
     4021 ttggaggtca acgtcgccat gaagtacatc atatacaata aatttcctta cattgggttc
     4081 ttgtcaaaaa aagtcgtcta tctg
//
LOCUS       DACDIG010000271         3208 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.guided.143, whole genome shotgun sequence.
ACCESSION   DACDIG010000271 DACDIG010000000
VERSION     DACDIG010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.guided.143"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            289..753
                     /locus_tag="IV247_003065"
     CDS             289..753
                     /locus_tag="IV247_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8779033.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            737..1366
                     /locus_tag="IV247_003066"
     CDS             737..1366
                     /locus_tag="IV247_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP8779034.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            1720..3198
                     /gene="msr"
                     /locus_tag="IV247_003067"
     CDS             1720..3198
                     /gene="msr"
                     /locus_tag="IV247_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP8779035.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
BASE COUNT         1109 a          528 c          661 g          910 t
ORIGIN      
        1 tcgacgggat tcttgcaaaa ggcgatttcg gaaatttttg gcggtgtaaa attatctact
       61 gcagaagagc tatcttctat agtttgataa gaatcaataa tcttggtttt agcgttgtgc
      121 tttctttatc attttttccg ttcattctac aaaaaatttc ttttctctat acttttctat
      181 tgagagcgtt ttttttatta cacagctgat tctttgactt ttttcttttt ttattctatt
      241 attgacaagt caatcgttga cgagtcaatt attagaaagg aggaaattat gtctttagaa
      301 gagattagtc accttttata tcaaatcaaa ttagccgatc aaaaaatcgt ccagatcttt
      361 gaagaaaata tcggtataag tcttactaga tacgagatca tgatgagact gaaagaagaa
      421 aatccattgc tacaaagcag tttgcaagaa aagttgcaga ttgatcaagg agcagttact
      481 cgtcatctga aggtattgga agcaaatggc tatgtttcgc gtcagagaaa tcctcagaac
      541 aaccgggaag tgtatgtagc gctaacagaa aaagcccaga agaaaatcaa gcattgtgaa
      601 atggaccata aagatacagg ttccgtgcta tccccggctt tcagcgatgt agaaatgaaa
      661 caatttctaa cacttttaga taaattgaat acccaacttt gcaatataac aacagaaacg
      721 aaagaggtta ataataatgg aacaaaaaaa tgatttttca acaattatga ctggtagaag
      781 atccgttcgc gtatatgatg aaacatacaa aatcccacac gaagaaatgc tggaaatgat
      841 caaagaagcg acgactgcac cttcttctgt caatatgaaa ccatggcgct ttgtggtcgc
      901 tgaaagcgag gaagcaaaag aaatcttgcg tcctttgatc cgtttcaata cgagacaaaa
      961 tgatacttct tctgcaatgg tcatgatttt tggcgatatg caatgttacg aatatggcga
     1021 agaaatctac aaccaagctt acgaatcagg gaagatgcca aaagaagtca gagatcaaca
     1081 attagctgcg atcattcctt attacaagag tttttcaaga gaagaaatga acgatgtagt
     1141 caaagtcgat tctagcttag cggctatgca attcatgtta gttgcgcgtg atcatggcta
     1201 tgaaacaaat ccaattggtg gattcgaagc agatcagttg gcagaggcat ttggtttgga
     1261 taaagaccgt tatgttccag ttattatttt atcaatagga aaagcggtag aagaaggtta
     1321 cgagtctgtt cgtttagcac cagaaaagat cactatgttc gaataaaagg ttttcagtta
     1381 ctattcagta gctattgctt tttttgaatc gtgtgtggta acctaataaa aaaggaggtc
     1441 ggaaaaatga ctgcatcgat gaaattacgt ttcgaacttt tgaataacaa ctaaataaat
     1501 tcaaaagaca tgtataaaca acaatttcat ggatggagta tgtccatttt ttcgaacgac
     1561 agtagaatag gtgaaagcta tgacagattt ggtgatttag tcatagttat ttcgttcaga
     1621 gtaggaaaag atgtgatgca gtttttttgc aaacagattt ttctgctctt ttttagtatc
     1681 gataattgtt gttttacatg aaataggagt gtaaaacata tggaaaattt agcagtaaat
     1741 ataacaaatc tacaagtcag ctttggcaac cagctagaac tatctattga ttctcttcgt
     1801 gtctatcagc aagatcgtat agggatcata ggagaaaatg gtgtcggtaa atcgactttg
     1861 ctcaaactaa tagccggtga actttttccc gatcatggga aaatccaaac agagatcacc
     1921 ttcaactacc tgcctcaatt aacctatctc gctgaagcaa aggacctaaa tttggaatta
     1981 gccagtcatt tccagttaag actggaagaa acttcagagc ggaaatggag cggaggggaa
     2041 gaacgaaaga tcgagttgat acgtcttctt tcttcttatg aacaagggat gcttctagac
     2101 gagccaacaa cccatctaga tagaaaaagt attgatcgac tgattgaaga gcttcgttat
     2161 tattatggta cgctggtttt tgttagtcat gatcgctatt ttctagatga gctagcatcg
     2221 aaaatctggg aagtaaaaga cggagaaatc cgagagtttt cggggaatta tagtgcctat
     2281 ctcactcaaa aggaattgga gaaaaagact cagctacgag aagcagagtc gatcatgaaa
     2341 gagaaaaaac gattggaaaa atcgatccaa gaaaagaaaa aacaagcgga aaagttggaa
     2401 aaagtgtcca gtaaaaagaa aaagcaacaa atcagactgg atcggttgtc ttcctctaaa
     2461 caaaaagaca gtgtacaaaa agccatccaa aagaatgcga aaacattaga gagaagactc
     2521 caaaaaatag gagaaacaac caagccgcag cagatgaaac aaatccgttt tccagtacca
     2581 aaatctcttg aactccacag ccgctatccc atcatgggac aaaatgtcca attggaaaga
     2641 agtgggagaa cattactggt aaatggtgat tttcagtttt ctttaggtaa aaaaatcgcg
     2701 attgtcggtg aaaatggttc aggtaagaca accttattag aacatatccg caaacaagga
     2761 gaaggaatcc ttctctctcc gaaagtaagc tttcaagtat atcagcaaaa gggttatcaa
     2821 atgacatctg aagaatccat cattcgtttt gtcatgagac aaacagagtt ttcagaatcg
     2881 cttgtccgta gtttgctgaa tcacttaggg tttgctcagg aaactttgac gaaaccgtta
     2941 tgtacattaa gtgggggaga agcgacccgt ctgacgattg ctttgctttt tactaagcca
     3001 agtaatgtgt tgctgttaga tgaaccgact aattttattg atatggcaac gatcgaagct
     3061 ttagagaagc taatgcaaat atatccggga acgattttgt ttacttctca tgattcttac
     3121 tttgtcgagc gtacggctga tgaagtttat gaaataaaag ggcagaaaat aaaaaaagta
     3181 cttacgagaa atttttaaat gtgctacg
//
LOCUS       DACDIG010000272         3208 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.guided.144, whole genome shotgun sequence.
ACCESSION   DACDIG010000272 DACDIG010000000
VERSION     DACDIG010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.guided.144"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            289..753
                     /locus_tag="IV247_003068"
     CDS             289..753
                     /locus_tag="IV247_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8779036.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            737..1366
                     /locus_tag="IV247_003069"
     CDS             737..1366
                     /locus_tag="IV247_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP8779037.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            1720..3198
                     /gene="msr"
                     /locus_tag="IV247_003070"
     CDS             1720..3198
                     /gene="msr"
                     /locus_tag="IV247_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP8779038.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
BASE COUNT         1108 a          528 c          662 g          910 t
ORIGIN      
        1 tcgacgggat tcttgcaaaa ggcgatttcg gaaatttttg gcggtgtaaa attatctact
       61 gcagaagagc tatcttctat agtttgataa gaatcaataa tcttggtttt agcgttgtgc
      121 tttctttatc attttttccg ttcattctac aaaaaatttc ttttctctat acttttctat
      181 tgagagcgtt ttttttatta cacagctgat tctttgactt ttttcttttt ttattctatt
      241 attgacaagt caatcgttga cgagtcaatt attagaaagg aggaaattat gtctttagaa
      301 gagattagtc accttttata tcaaatcaaa ttagccgatc aaaaaatcgt ccagatcttt
      361 gaagaaaata tcggtataag tcttactaga tacgagatca tgatgagact gaaagaagaa
      421 aatccattgc tacaaagcag tttgcaagaa aagttgcaga ttgatcaagg agcagttact
      481 cgtcatctga aggtattgga agcaaatggc tatgtttcgc gtcagagaaa tcctcagaac
      541 aaccgggaag tgtatgtagc gctaacagaa aaagcccaga agaaaatcaa gcattgtgaa
      601 atggaccata aagatacagg ttccgtgcta tccccggctt tcagcgatgt agaaatgaaa
      661 caatttctaa cacttttaga taaattgaat acccaacttt gcaatataac aacagaaacg
      721 aaagaggtta ataataatgg aacaaaaaaa tgatttttca acaattatga ctggtagaag
      781 atccgttcgc gtatatgatg aaacatacaa aatcccacac gaagaaatgc tggaaatgat
      841 caaagaagcg acgactgcac cttcttctgt caatatgaaa ccatggcgct ttgtggtcgc
      901 tgaaagcgag gaagcaaaag aaatcttgcg tcctttgatc cgtttcaata cgagacaaaa
      961 tgatacttct tctgcaatgg tcatgatttt tggcgatatg caatgttacg aatatggcga
     1021 agaaatctac aaccaagctt acgaatcagg gaagatgcca aaagaagtca gagatcaaca
     1081 attagctgcg atcattcctt attacaagag tttttcaaga gaagaaatga acgatgtagt
     1141 caaagtcgat tctagcttag cggctatgca attcatgtta gttgcgcgtg atcatggcta
     1201 tgaaacaaat ccaattggtg gattcgaagc agatcagttg gcagaggcat ttggtttgga
     1261 taaagaccgt tatgttccag ttattatttt atcaatagga aaagcggtag aagaaggtta
     1321 cgagtctgtt cgtttagcac cagaaaagat cactatgttc gaataaaagg ttttcagtta
     1381 ctattcagta gctattgctt tttttgaatc gtgtgtggta acctaataaa aaaggaggtc
     1441 ggaaaaatga ctgcatcgat gaaattacgt ttcgaacttt tgaataacaa ctaaataaat
     1501 tcaaaagaca tgtataaaca acaatttcat ggatggagta tgtccatttt ttcgaacgac
     1561 agtagaatag gtgaaagcta tgacagattt ggtgatttag tcatagttat ttcgttcaga
     1621 gtaggaaaag atgtgatgca gtttttttgc aaacagattt ttctgctctt ttttagtatc
     1681 gataattgtt gttttacatg aaataggagt gtaaaacata tggaaaattt agcagtaaat
     1741 ataacaaatc tacaagtcag ctttggcaac cagctagaac tatctattga ttctcttcgt
     1801 gtctatcagc aagatcgtat agggatcata ggagaaaatg gtgtcggtaa atcgactttg
     1861 ctcaaactaa tagccggtga actttttccc gatcatggga aaatccaaac agagatcacc
     1921 ttcaactacc tgcctcaatt aacctatctc gctgaagcaa aggacctaaa tttggaatta
     1981 gccagtcatt tccagttaag actggaagaa acttcagagc ggaaatggag cggaggggaa
     2041 gaacgaaaga tcgagttgat acgtcttctt tcttcttatg aacaagggat gcttctagac
     2101 gagccaacaa cccatctaga tagaaaaagt attgatcgac tgattgaaga gcttcgttat
     2161 tattatggta cgctggtttt tgttagtcat gatcgctatt ttctagatga gctagcatcg
     2221 aaaatctggg aagtaaaaga cggagaaatc cgagagtttt cggggaatta tagtgcctat
     2281 ctcactcaaa aggaattgga gaaaaagact cagctacgag aagcagagtc gatcatgaaa
     2341 gagaaaaaac gattggaaaa atcgatccaa gaaaagaaaa aacaagcgga aaagttggaa
     2401 aaagtgtcca gtaaaaagaa aaagcaacaa atcagactgg atcggttgtc ttcctctaaa
     2461 caaaaagaca gtgtacaaaa agccatccaa aagaatgcga aaacattaga gagaagactc
     2521 caaaaaatag gagaaacaac caagccgcag cagatgaaac aaatccgttt tccagtacca
     2581 aaatctcttg aactccacag ccgctatccc atcatgggac aaaatgtcca attggaaaga
     2641 agtgggagaa cattactggt aaatggtgat tttcagtttt ctttaggtaa aaaaatcgcg
     2701 attgtcggtg aaaatggttc aggtaagaca accttattag aacatatccg caaacaagga
     2761 gaaggaatcc ttctctctcc gaaagtaagc tttcaagtat atcagcaaaa gggttatcaa
     2821 atgacatctg aagaatccat cattcgtttt gtcatgagac aaacagagtt ttcagaatcg
     2881 cttgtccgta gtttgctgaa tcacttaggg tttgctcagg aaactttgac gaaaccgtta
     2941 tgtacattaa gtgggggaga agcgacccgt ctgacgattg ctttgctttt tactaagcca
     3001 agtaatgtgt tgctgttaga tgaaccgact aattttattg atatggcaac gatcgaagct
     3061 ttagagaagc taatgcaaat atatccggga acgattttgt ttacttctca tgattcttac
     3121 tttgtcgagc gtacggctga tgaagtttat gaaataaaag ggcagaagat aaaaaaagta
     3181 cttacgagaa atttttaaat gtgctacg
//
LOCUS       DACDIG010000273         2674 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.guided.150, whole genome shotgun sequence.
ACCESSION   DACDIG010000273 DACDIG010000000
VERSION     DACDIG010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.guided.150"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..94
                     /locus_tag="IV247_003071"
     CDS             <1..94
                     /locus_tag="IV247_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP8779039.1"
                     /translation="EKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            114..641
                     /locus_tag="IV247_003072"
     CDS             114..641
                     /locus_tag="IV247_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8779040.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(704..1357)
                     /locus_tag="IV247_003073"
     CDS             complement(704..1357)
                     /locus_tag="IV247_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP8779041.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            1489..2331
                     /locus_tag="IV247_003074"
     CDS             1489..2331
                     /locus_tag="IV247_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8779042.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            2489..>2674
                     /locus_tag="IV247_003075"
     CDS             2489..>2674
                     /locus_tag="IV247_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HAP8779043.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLT"
BASE COUNT          967 a          483 c          528 g          696 t
ORIGIN      
        1 ggaaaagcag tcagtatttg attttgagat ggaagatatc caattagaag gatatgaccc
       61 acatccggcg atcaaagctc ctatcgctgt ttagaatcag aaaggatgag aaaatgttag
      121 ctgcaatttg ggcacaggat gaaaacggat tgattggaaa agaagaccgt cttccttggc
      181 gtttgccaaa tgatctgaaa ttcttcaaac aaatgacaga agcaaataca ctagtgatgg
      241 gacgaaaaac attcgaaggg atgggtagcc gtccgttgcc gaatcgtcag acgattgttt
      301 tgacgagaga ttcttcctat aaagcagaag gcgtgcatgt catgcatagt gtggaagacg
      361 tgcttgctct ggaaaaagaa acagacggta tctttttcat cgctggaggg tcagctgttt
      421 atcaagaatt tctgccattt tgtacgatat tataccgtac agtcatccat catgtattcg
      481 atggagatgc ttattttcca cctgttgact ggtctgattg gtcactgatc aatactagtc
      541 aaggagaaat ggatgaaaaa aatcgttatc cgcatcaatt tgaaacgtat cagcgtaatg
      601 agaatcctgt aaaaagcggg acaggcgaat tgacgatata aaaaagctat ggatagacta
      661 gctgttattt agagctgtcc catccatagc tttatttttt ttattaagta aagaataaaa
      721 cagagaagta cataaaacct gcacctaaca taacaaacaa atgccaaaca acatgcatat
      781 agcggataga attcaagcta taaaagaatg cgcctatcgt gtaagaaagc ccgcctgcta
      841 ccatcaaaat aacaccagtc attccaagag agtgataaag aggaacagca gccacgacac
      901 agagccaccc cataaaaata tagatgatcg tagatacttt tgatactctc tcccgtttat
      961 gcaacgtcaa cgctttatat ataatgccaa gaatcgcaaa caaccagatt aatacaaata
     1021 aagtccaacc tagccatcct cggatactca gcaaacaaaa aggagtataa cttcctgcaa
     1081 tcaaaagaaa aatcgaacta tgatcgaata cttggaacac cttttttgct tttgtaaaaa
     1141 tcaatgaatg aaacaaagta gacgttaaaa ataaaagaat catcatcgat ccatagatag
     1201 cataagagac aatatggagg gaggaatgca agtgggcacc ttttactaaa agaaccacta
     1261 gtcccgcgat actcaatcca gtcccaattc catgggtgac tgcattaaac acttcattta
     1321 atatttcata cgttcgatta aatttcgttt tttccatgaa aacggctcct ttactaggtg
     1381 taattaatga aaaatcatta tttgtctgtt atactaaaaa tagaaattgt cttacatgta
     1441 gtttaacatg aataaggcat cttgttaaag aaagagagag agaaaataat gacaaatgta
     1501 aaaatcgtaa cagattcttc atgtacgatg gaaaaaaatg tacgcgatgc tttaggcatt
     1561 cgtgtcatgc cgctatccat catgatcgac ggaacagtat atcccgatga tgaccaacta
     1621 gacggcgaaa gatttatgga aatgatggcg aaagcaaaaa atctgcccaa aacaagccaa
     1681 ccaccaattg gagaattcat ctcgatgtat gatgagctag gagaagacgg cagtgaagtg
     1741 atttcgatcc atatgacaaa aggcttgagt ggaacagtag aagcagcaag acaagccagt
     1801 aatctaacaa aaacgaaagt aacggttatc gacagtgata ccactgacca aggattatct
     1861 tttcaggtaa ttcgagcagc tgaattagca aaagaaggta aaacagtaga gaaaatcctt
     1921 gaagaagtca aaaaagtaca agacaataca aaattataca tcggaatatc cacactagat
     1981 aacttggtaa aaggtggaag aatcagccga acaactggtt tgctgtctag tgttttgaac
     2041 atacgcgtgg tgatgaattt cgcccattct gaattaatcc ctgtaactaa ggggcgagga
     2101 aagaaaacgt tcgataaatg gtttaataat ttgaaaaatg aactgaaaga attgccaaat
     2161 gtgcgacaaa tcggtatttc acatgcggat gcaaaagaat tggcgcaaga attcaaagag
     2221 ggtttgcaag aattattccc ggacatgcat atccctgtac tgcatacgaa tccggttatc
     2281 gctacgcata ctggtaaaga cgcgtttgca atcatgtatt atgtagatta gtcgaaaaga
     2341 ggataagccc tcagcctgaa aggcttgcag cttattctct ttttctttat aaaattagga
     2401 aaataattgt tttctttttt cataaagact acctttttga taaaattttt gataacatta
     2461 gaaccaatcg aatacaagag gtgaaaaaat gaaattaacg aaacgacatt tgatcctcgt
     2521 tttgttccca ttattaattg cgatcatcat ctatgcaggt ttagctgctt ctataccgaa
     2581 agctggccca ctaatgaaac cagtaactac tgtgagagaa gatccaaata aaaaagaaaa
     2641 acttcactac atagctatcg gagattcgct gact
//
LOCUS       DACDIG010000274         2292 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.guided.164, whole genome shotgun sequence.
ACCESSION   DACDIG010000274 DACDIG010000000
VERSION     DACDIG010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.guided.164"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..774
                     /locus_tag="IV247_003076"
     CDS             <1..774
                     /locus_tag="IV247_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAP8779044.1"
                     /translation="VCHWIWGGGFLDQMGFRDFAGGAVIHTTAGFGSLACILTLGQRK
                     IPLGERSQHSSLMAAAIGTGLLWFGWFGFNSGGALRADEQAVNAFGSTFIALAFAMIT
                     WLIIAKVKGNGFDFVDVLTGSVAGLAAITPCAGYVEAKSAMLIGIIAGIVCHAAVDFR
                     KKKQWDDALDVWGVHGMGGFTGTILIGIFASNSHLLTNPSSWYFLGIQVIGVIITAVY
                     AYVLTTVILKSAMHFTTITTTKEEQEEGLDFFFFGNKLN"
     gene            805..1023
                     /locus_tag="IV247_003077"
                     /pseudo
     CDS             805..1023
                     /locus_tag="IV247_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1442..1915
                     /gene="dfrF"
                     /locus_tag="IV247_003078"
     CDS             1442..1915
                     /gene="dfrF"
                     /locus_tag="IV247_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HAP8779045.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            2042..>2292
                     /locus_tag="IV247_003079"
     CDS             2042..>2292
                     /locus_tag="IV247_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAP8779046.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVV"
BASE COUNT          738 a          337 c          516 g          701 t
ORIGIN      
        1 gtttgccact ggatatgggg tggcggattt ttggatcaga tgggatttag agatttcgct
       61 ggtggagcag tcatccacac gacagctgga ttcggctctt tagcatgtat cctcacactt
      121 ggtcaaagaa agattccttt aggagaaaga agtcagcata gcagtcttat ggcagctgct
      181 atcggtacag gactgctttg gtttggctgg ttcggtttta attccggtgg tgcacttcga
      241 gcagatgaac aagcagtcaa tgcatttggt tcaacattca tcgctcttgc atttgcgatg
      301 attacttggc tgatcattgc aaaagtaaaa ggaaatggct ttgattttgt cgacgtacta
      361 acaggatccg ttgctggatt ggcagccatt acgccttgtg ccggttatgt agaagcaaaa
      421 agtgccatgc tgattgggat cattgcgggt atcgtctgtc atgctgctgt tgattttcgt
      481 aaaaagaaac aatgggatga tgcacttgat gtgtggggtg ttcatggcat gggcggattc
      541 acaggtacga ttctgatagg gatctttgca tctaacagcc atttactaac gaatccaagc
      601 agctggtact tcttagggat tcaagtgatt ggtgtcatca ttactgccgt gtatgcctat
      661 gtgctgacaa cagtgatttt aaaatcggcg atgcacttta caacgatcac tactacaaaa
      721 gaagaacaag aagaaggatt ggactttttc ttctttggca acaaactcaa ttgaaacaaa
      781 ttcaaataga aaagaggaag attcatgcaa caaagcaaaa cgaaaattcc tgcaaaaaaa
      841 ttaacgttct ttggcttttt ggctatgacc atctcaatgg tcgtttcatt atatgaatat
      901 cccacattcg cgacctcagg cttcagctta gtatttttct tgttattagg cggtttgcta
      961 tggtttatcc ctgtggcatt gtgcgcagca gagatggcaa cggtgaaagg atgggaaaaa
     1021 ggtgtttccc aaacaaaaag atattaagaa acagcccatg tttacctgct tgcaaacaaa
     1081 agaatattgc tactagacta agagatacta cataagtgtg ttggtatctc ttttttgttg
     1141 ccgtgaaaca gcagactatt tcatttctgc ttgattacct taaaacttat tagtctatgg
     1201 tttgtcaagg gcttgttgcg tgagaaatgc tgaatatcac cctttacaaa acataggctg
     1261 ataagtaact tttcaaaagc agaaaagaaa tggcaatgct tataacaata tgatataatg
     1321 cagatgaaga caaaaaagga atttgtcgag ctggttagtt cgagataatt ttgagtttgt
     1381 taagatgtgg tgcacttgat ggaatcacag gtacgacatc atattatgga ggatgaaata
     1441 gatgataggt ttgattgttg cgaggtcaaa gaataatgtt ataggcaaga atggtaatat
     1501 accatggaaa ataaagggag aacaaaagca atttagagag ttaacaacgg gtaatgtggt
     1561 tattatgggg cgaaagtctt atgaagaaat cggtcatccg ttgcctaata gaatgaatat
     1621 tgttgtttcc accacaacag agtatcaagg agataattta gtttcagtta aatcattaga
     1681 agatgcatta ttattggcta aaggacgaga tgtatacata tctggtggat atggactatt
     1741 taaggaagct ttgcaaatag tagataaaat gtatatcaca gaagtagatt taaatattga
     1801 agatggagat acattctttc cagaatttga tatcaatgat tttgaagttt tgatagggga
     1861 aacacttggt gaggaagtga aatatacgag aacattttat gtaaggaaaa aatgaattga
     1921 gtaaattttg gatttagggt gttttcataa atatattgct ttttatgcat atataaattg
     1981 ttgtaagaca aattccagtt tgtatggggc gataaaatca gaatttgaga gaggattgaa
     2041 tatgaataag gaatggtctg aacttaataa aacaatgcag gcacaaataa aaaagaaaga
     2101 tacttataag aggggtattg atactttact tactttacga agtcagttaa tacaaacctt
     2161 agtatctttc aaagaagaat tatgcagaga agattttaac tcaatcccct tcataaacgc
     2221 tgatggttat catagtaaga cgattgctta ttctatttgg catattttcc gcattgaaga
     2281 tatagttgtg ca
//
LOCUS       DACDIG010000275         1435 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.guided.192, whole genome shotgun sequence.
ACCESSION   DACDIG010000275 DACDIG010000000
VERSION     DACDIG010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.guided.192"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            262..373
                     /locus_tag="IV247_003080"
                     /pseudo
     CDS             262..373
                     /locus_tag="IV247_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787626.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            394..477
                     /locus_tag="IV247_003081"
     CDS             394..477
                     /locus_tag="IV247_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAP8779047.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            602..1339
                     /gene="erm(B)"
                     /locus_tag="IV247_003082"
     CDS             602..1339
                     /gene="erm(B)"
                     /locus_tag="IV247_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP8779048.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          519 a          214 c          240 g          462 t
ORIGIN      
        1 atttgtatcg ataagaaata gatttaaaaa tttcgctgtt attttgtaca tttaacttga
       61 cggtgacatc tctctattgt gagttattag tggtacagtt ttcaaccgtt ttaattataa
      121 aaaagtggtg catttttaaa ttggcacaaa caggtaacgg ttattgcagg tgtatttctt
      181 atctatgggt ttaacatgga ttttatcatt aaaatcatga gtattgtccg agagtgattg
      241 gtcttgcgta tggttaaccc taaagttatg gaaataagac ttagaagcaa acttaagagt
      301 gtgttgatag tgcattatct taaaattttg tataatagga attgaagtta aattagatgc
      361 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgttatgta
      421 gataaaacat ctactgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      481 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      541 acaaacaaat cgtttaactt ctgtatttgt ttatagatgt aatcacttca ggagtgataa
      601 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      661 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      721 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      781 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat
      841 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      901 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccgtgcgtc
      961 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
     1021 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
     1081 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
     1141 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
     1201 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
     1261 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
     1321 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
     1381 ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta ccaat
//
LOCUS       DACDIG010000276         1349 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.guided.196, whole genome shotgun sequence.
ACCESSION   DACDIG010000276 DACDIG010000000
VERSION     DACDIG010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.guided.196"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            46..297
                     /locus_tag="IV247_003083"
                     /pseudo
     CDS             46..297
                     /locus_tag="IV247_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            390..1184
                     /locus_tag="IV247_003084"
                     /note="aph(3')-IIIa"
     CDS             390..1184
                     /locus_tag="IV247_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP8779049.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          424 a          234 c          322 g          369 t
ORIGIN      
        1 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
       61 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      121 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      181 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      241 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      301 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      361 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      421 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      481 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      541 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      601 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      661 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      721 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      781 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      841 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      901 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
      961 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1021 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1081 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1141 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1201 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1261 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1321 cgcctacgag gaatttgtat cgataagaa
//
LOCUS       DACDIG010000277          645 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63702_2016
            SAMN12640246-rid9892033.guided.250, whole genome shotgun sequence.
ACCESSION   DACDIG010000277 DACDIG010000000
VERSION     DACDIG010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640246
            Sequence Read Archive: SRR10040963
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742907.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742907.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:51:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,017
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 28 of 69
            Pseudo Genes (incomplete)         :: 34 of 69
            Pseudo Genes (internal stop)      :: 16 of 69
            Pseudo Genes (multiple problems)  :: 8 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640246-rid9892033.guided.250"
                     /isolate="E63702_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            32..580
                     /gene="aac(6')"
                     /locus_tag="IV247_003085"
     CDS             32..580
                     /gene="aac(6')"
                     /locus_tag="IV247_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP8779050.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          235 a          103 c          147 g          160 t
ORIGIN      
        1 ttagaataag caagaacaag ggggaagacg tatgataatc agtgaatttg accgtaataa
       61 tccagtattg aaagatcagc tttctgattt actgagactg acttggccgg aagaatatgg
      121 agacagctcg gcagaagaag tagaagaaat gatgaatcca gaacgaatcg cggtagcagc
      181 ggtagaccaa gatgagttag taggatttat tggtgcaatc cctcaatacg gtatcacagg
      241 ttgggaattg catccattag ttgtagaaag ctcccgacga aagaaccaaa taggtactcg
      301 attagtcaat tacttagaaa aagaagtagc ttccagagga ggaatcacga tttatttagg
      361 tacggatgat ttagaccatg gaacaacgtt aagtcaaacg gacctgtatg agcatacatt
      421 tgataaagtg gcttccatcc agaaccttcg tgaacatccg tatgaattct atgaaaaatt
      481 aggttataaa atcgtaggtg tcttaccaaa tgcaaatggc tgggacaaac cggatatttg
      541 gatggcaaaa acgattattc ctcgaccaaa ttctcaataa taaaaagagg ttgtgacaga
      601 agtaatcagc ttcaagaaat aagaaggaat ttacgaaaat tactt
//
